LOCUS       DAJTRQ010000001       141661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000001 DAJTRQ010000000
VERSION     DAJTRQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 141661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO332_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO332_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5940634.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO332_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO332_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5940635.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO332_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO332_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO332_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO332_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5940636.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO332_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO332_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5940637.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO332_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO332_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5940638.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO332_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO332_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5940639.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO332_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO332_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5940640.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO332_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO332_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5940641.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO332_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO332_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5940642.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO332_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO332_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5940643.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO332_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO332_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5940644.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO332_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO332_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5940645.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO332_000014"
     CDS             18619..18894
                     /locus_tag="OO332_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940646.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO332_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO332_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5940647.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO332_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO332_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5940648.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO332_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO332_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5940649.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO332_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO332_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5940650.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /gene="ypdA"
                     /locus_tag="OO332_000019"
     CDS             22592..24289
                     /gene="ypdA"
                     /locus_tag="OO332_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR5940651.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..25037
                     /gene="ypdB"
                     /locus_tag="OO332_000020"
                     /pseudo
     CDS             24304..25037
                     /gene="ypdB"
                     /locus_tag="OO332_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            25050..25907
                     /locus_tag="OO332_000021"
     CDS             25050..25907
                     /locus_tag="OO332_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5940652.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="OO332_000022"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="OO332_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5940653.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="OO332_000023"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="OO332_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5940654.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="OO332_000024"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="OO332_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="OO332_000025"
     CDS             complement(30571..31818)
                     /locus_tag="OO332_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5940655.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="OO332_000026"
     CDS             complement(31840..32166)
                     /locus_tag="OO332_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5940656.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="OO332_000027"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="OO332_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5940657.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="OO332_000028"
     CDS             33554..34810
                     /locus_tag="OO332_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5940658.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="OO332_000029"
     CDS             34925..35251
                     /locus_tag="OO332_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5940659.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35392..36630)
                     /locus_tag="OO332_000030"
     CDS             complement(35392..36630)
                     /locus_tag="OO332_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5940660.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36966..38168
                     /gene="nupC"
                     /locus_tag="OO332_000031"
     CDS             36966..38168
                     /gene="nupC"
                     /locus_tag="OO332_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5940661.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38218..40407)
                     /locus_tag="OO332_000032"
                     /pseudo
     CDS             complement(38218..40407)
                     /locus_tag="OO332_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40615..40690)
                     /locus_tag="OO332_000033"
     tRNA            complement(40615..40690)
                     /locus_tag="OO332_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40655..40657),aa:Ala,seq:ggc)
     gene            complement(40730..40805)
                     /locus_tag="OO332_000034"
     tRNA            complement(40730..40805)
                     /locus_tag="OO332_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40770..40772),aa:Ala,seq:ggc)
     gene            41026..41385
                     /locus_tag="OO332_000035"
     CDS             41026..41385
                     /locus_tag="OO332_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5940662.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41387..41779
                     /locus_tag="OO332_000036"
     CDS             41387..41779
                     /locus_tag="OO332_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5940663.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="OO332_000037"
     CDS             complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="OO332_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5940664.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43505..43580
                     /locus_tag="OO332_000038"
     tRNA            43505..43580
                     /locus_tag="OO332_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43538..43540,aa:Val,seq:tac)
     gene            43625..43700
                     /locus_tag="OO332_000039"
     tRNA            43625..43700
                     /locus_tag="OO332_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43658..43660,aa:Val,seq:tac)
     gene            43705..43780
                     /locus_tag="OO332_000040"
     tRNA            43705..43780
                     /locus_tag="OO332_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43738..43740,aa:Lys,seq:ttt)
     gene            43811..43936
                     /locus_tag="OO332_000041"
     ncRNA           43811..43936
                     /ncRNA_class="other"
                     /locus_tag="OO332_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44056..44397
                     /locus_tag="OO332_000042"
     CDS             44056..44397
                     /locus_tag="OO332_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5940665.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44388..45314)
                     /locus_tag="OO332_000043"
     CDS             complement(44388..45314)
                     /locus_tag="OO332_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5940666.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45404..46402
                     /locus_tag="OO332_000044"
     CDS             45404..46402
                     /locus_tag="OO332_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5940667.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46399..46617)
                     /locus_tag="OO332_000045"
     CDS             complement(46399..46617)
                     /locus_tag="OO332_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5940668.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46619..48634)
                     /gene="ligA"
                     /locus_tag="OO332_000046"
     CDS             complement(46619..48634)
                     /gene="ligA"
                     /locus_tag="OO332_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5940669.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48705..49592)
                     /gene="zipA"
                     /locus_tag="OO332_000047"
     CDS             complement(48705..49592)
                     /gene="zipA"
                     /locus_tag="OO332_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5940670.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            49822..50583
                     /gene="cysZ"
                     /locus_tag="OO332_000048"
     CDS             49822..50583
                     /gene="cysZ"
                     /locus_tag="OO332_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5940671.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50768..51739
                     /gene="cysK"
                     /locus_tag="OO332_000049"
     CDS             50768..51739
                     /gene="cysK"
                     /locus_tag="OO332_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5940672.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52123..52380
                     /gene="ptsH"
                     /locus_tag="OO332_000050"
     CDS             52123..52380
                     /gene="ptsH"
                     /locus_tag="OO332_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5940673.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52425..54152
                     /gene="ptsI"
                     /locus_tag="OO332_000051"
     CDS             52425..54152
                     /gene="ptsI"
                     /locus_tag="OO332_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5940674.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54193..54702
                     /gene="crr"
                     /locus_tag="OO332_000052"
     CDS             54193..54702
                     /gene="crr"
                     /locus_tag="OO332_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5940675.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54744..55595)
                     /gene="pdxK"
                     /locus_tag="OO332_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54744..55595)
                     /gene="pdxK"
                     /locus_tag="OO332_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5940676.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55700..56074
                     /locus_tag="OO332_000054"
     CDS             55700..56074
                     /locus_tag="OO332_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5940677.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56107..56830
                     /locus_tag="OO332_000055"
                     /pseudo
     CDS             56107..56830
                     /locus_tag="OO332_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(57034..57945)
                     /gene="cysM"
                     /locus_tag="OO332_000056"
     CDS             complement(57034..57945)
                     /gene="cysM"
                     /locus_tag="OO332_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5940678.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58079..59176)
                     /gene="cysA"
                     /locus_tag="OO332_000057"
     CDS             complement(58079..59176)
                     /gene="cysA"
                     /locus_tag="OO332_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5940679.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59166..60041)
                     /gene="cysW"
                     /locus_tag="OO332_000058"
     CDS             complement(59166..60041)
                     /gene="cysW"
                     /locus_tag="OO332_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5940680.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(60041..60874)
                     /gene="cysT"
                     /locus_tag="OO332_000059"
     CDS             complement(60041..60874)
                     /gene="cysT"
                     /locus_tag="OO332_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5940681.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60874..61890)
                     /gene="cysP"
                     /locus_tag="OO332_000060"
     CDS             complement(60874..61890)
                     /gene="cysP"
                     /locus_tag="OO332_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5940682.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62194..62985)
                     /gene="ucpA"
                     /locus_tag="OO332_000061"
     CDS             complement(62194..62985)
                     /gene="ucpA"
                     /locus_tag="OO332_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5940683.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63172..63972)
                     /gene="murR"
                     /locus_tag="OO332_000062"
     CDS             complement(63172..63972)
                     /gene="murR"
                     /locus_tag="OO332_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5940684.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64136..65032
                     /gene="murQ"
                     /locus_tag="OO332_000063"
     CDS             64136..65032
                     /gene="murQ"
                     /locus_tag="OO332_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5940685.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            65036..66460
                     /gene="murP"
                     /locus_tag="OO332_000064"
     CDS             65036..66460
                     /gene="murP"
                     /locus_tag="OO332_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5940686.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66640..67539)
                     /gene="yfeX"
                     /locus_tag="OO332_000065"
     CDS             complement(66640..67539)
                     /gene="yfeX"
                     /locus_tag="OO332_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5940687.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67635..68210)
                     /locus_tag="OO332_000066"
     CDS             complement(67635..68210)
                     /locus_tag="OO332_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5940688.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68271..68720)
                     /locus_tag="OO332_000067"
     CDS             complement(68271..68720)
                     /locus_tag="OO332_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5940689.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68707..69132)
                     /locus_tag="OO332_000068"
     CDS             complement(68707..69132)
                     /locus_tag="OO332_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5940690.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69346..70215
                     /gene="amiA"
                     /locus_tag="OO332_000069"
     CDS             69346..70215
                     /gene="amiA"
                     /locus_tag="OO332_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5940691.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70219..71118
                     /gene="hemF"
                     /locus_tag="OO332_000070"
     CDS             70219..71118
                     /gene="hemF"
                     /locus_tag="OO332_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5940692.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71052..72176)
                     /gene="eutR"
                     /locus_tag="OO332_000071"
     CDS             complement(71052..72176)
                     /gene="eutR"
                     /locus_tag="OO332_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5940693.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(72261..72722)
                     /gene="eutK"
                     /locus_tag="OO332_000072"
     CDS             complement(72261..72722)
                     /gene="eutK"
                     /locus_tag="OO332_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5940694.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72735..73394)
                     /gene="eutL"
                     /locus_tag="OO332_000073"
     CDS             complement(72735..73394)
                     /gene="eutL"
                     /locus_tag="OO332_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5940695.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73404..74291)
                     /gene="eutC"
                     /locus_tag="OO332_000074"
     CDS             complement(73404..74291)
                     /gene="eutC"
                     /locus_tag="OO332_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR5940696.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74312..75673)
                     /gene="eutB"
                     /locus_tag="OO332_000075"
     CDS             complement(74312..75673)
                     /gene="eutB"
                     /locus_tag="OO332_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR5940697.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75862..77067
                     /locus_tag="OO332_000076"
     CDS             75862..77067
                     /locus_tag="OO332_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5940698.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77362..78309
                     /locus_tag="OO332_000077"
     CDS             77362..78309
                     /locus_tag="OO332_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5940699.1"
                     /translation="MYQSATAYVAKPGETLVKHKIATLQALQTLQALPEQVQAAFRDG
                     MNIIRFDGYRWEYKPATGFVRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQ
                     EQEQEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNE
                     EAEEDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDEL
                     KLYTWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQAL
                     RGKCGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            79360..80112
                     /locus_tag="OO332_000078"
     CDS             79360..80112
                     /locus_tag="OO332_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940700.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80872..81267
                     /locus_tag="OO332_000079"
     CDS             80872..81267
                     /locus_tag="OO332_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5940701.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81285..81506
                     /locus_tag="OO332_000080"
     CDS             81285..81506
                     /locus_tag="OO332_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940702.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            81571..82113
                     /locus_tag="OO332_000081"
     CDS             81571..82113
                     /locus_tag="OO332_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940703.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82578..82784
                     /locus_tag="OO332_000082"
     CDS             82578..82784
                     /locus_tag="OO332_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940704.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            83096..83581
                     /locus_tag="OO332_000083"
     CDS             83096..83581
                     /locus_tag="OO332_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5940705.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            83562..83840
                     /locus_tag="OO332_000084"
     CDS             83562..83840
                     /locus_tag="OO332_000084"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940706.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            83833..85293
                     /locus_tag="OO332_000085"
     CDS             83833..85293
                     /locus_tag="OO332_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5940707.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85327..86136
                     /gene="murI"
                     /locus_tag="OO332_000086"
     CDS             85327..86136
                     /gene="murI"
                     /locus_tag="OO332_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5940708.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86255..86946)
                     /locus_tag="OO332_000087"
     CDS             complement(join(86255..86700,86700..86946))
                     /locus_tag="OO332_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5940709.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(87470..88843)
                     /gene="eutA"
                     /locus_tag="OO332_000088"
     CDS             complement(87470..88843)
                     /gene="eutA"
                     /locus_tag="OO332_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5940710.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88818..89297)
                     /gene="eutP"
                     /locus_tag="OO332_000089"
     CDS             complement(88818..89297)
                     /gene="eutP"
                     /locus_tag="OO332_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5940711.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89310..89645)
                     /gene="eutS"
                     /locus_tag="OO332_000090"
     CDS             complement(89310..89645)
                     /gene="eutS"
                     /locus_tag="OO332_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5940712.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89938..92217)
                     /gene="maeB"
                     /locus_tag="OO332_000091"
     CDS             complement(89938..92217)
                     /gene="maeB"
                     /locus_tag="OO332_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5940713.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92506..93456
                     /gene="tal"
                     /locus_tag="OO332_000092"
     CDS             92506..93456
                     /gene="tal"
                     /locus_tag="OO332_000092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5940714.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93476..95479
                     /gene="tkt"
                     /locus_tag="OO332_000093"
     CDS             93476..95479
                     /gene="tkt"
                     /locus_tag="OO332_000093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5940715.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95593..96624)
                     /locus_tag="OO332_000094"
     CDS             complement(95593..96624)
                     /locus_tag="OO332_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5940716.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(96750..97325)
                     /gene="nudK"
                     /locus_tag="OO332_000095"
     CDS             complement(96750..97325)
                     /gene="nudK"
                     /locus_tag="OO332_000095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5940717.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97393..99372)
                     /gene="aegA"
                     /locus_tag="OO332_000096"
     CDS             complement(97393..99372)
                     /gene="aegA"
                     /locus_tag="OO332_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5940718.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            99578..101301
                     /gene="narQ"
                     /locus_tag="OO332_000097"
                     /pseudo
     CDS             99578..101301
                     /gene="narQ"
                     /locus_tag="OO332_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101503..104616
                     /gene="acrD"
                     /locus_tag="OO332_000098"
     CDS             101503..104616
                     /gene="acrD"
                     /locus_tag="OO332_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5940719.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104715..104774)
                     /gene="ypfM"
                     /locus_tag="OO332_000099"
     CDS             complement(104715..104774)
                     /gene="ypfM"
                     /locus_tag="OO332_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5940720.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            105155..105511
                     /locus_tag="OO332_000100"
     CDS             105155..105511
                     /locus_tag="OO332_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5940721.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            105515..106642
                     /gene="dapE"
                     /locus_tag="OO332_000101"
     CDS             105515..106642
                     /gene="dapE"
                     /locus_tag="OO332_000101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5940722.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            106670..106870
                     /locus_tag="OO332_000102"
     CDS             106670..106870
                     /locus_tag="OO332_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5940723.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(106980..107678)
                     /gene="ypfH"
                     /locus_tag="OO332_000103"
     CDS             complement(106980..107678)
                     /gene="ypfH"
                     /locus_tag="OO332_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5940724.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(107752..109767)
                     /gene="tmcA"
                     /locus_tag="OO332_000104"
     CDS             complement(107752..109767)
                     /gene="tmcA"
                     /locus_tag="OO332_000104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5940725.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(109782..110645)
                     /locus_tag="OO332_000105"
     CDS             complement(109782..110645)
                     /locus_tag="OO332_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5940726.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(110813..111526)
                     /gene="purC"
                     /locus_tag="OO332_000106"
     CDS             complement(110813..111526)
                     /gene="purC"
                     /locus_tag="OO332_000106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5940727.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(111739..112773)
                     /gene="bamC"
                     /locus_tag="OO332_000107"
     CDS             complement(111739..112773)
                     /gene="bamC"
                     /locus_tag="OO332_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5940728.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(112790..113668)
                     /gene="dapA"
                     /locus_tag="OO332_000108"
     CDS             complement(112790..113668)
                     /gene="dapA"
                     /locus_tag="OO332_000108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5940729.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            113814..114386
                     /locus_tag="OO332_000109"
     CDS             113814..114386
                     /locus_tag="OO332_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5940730.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            114386..114856
                     /gene="bcp"
                     /locus_tag="OO332_000110"
     CDS             114386..114856
                     /gene="bcp"
                     /locus_tag="OO332_000110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5940731.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            115109..115726
                     /gene="hyfA"
                     /locus_tag="OO332_000111"
     CDS             115109..115726
                     /gene="hyfA"
                     /locus_tag="OO332_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5940732.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            115726..117743
                     /gene="hyfB"
                     /locus_tag="OO332_000112"
                     /pseudo
     CDS             115726..117743
                     /gene="hyfB"
                     /locus_tag="OO332_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            117754..118695
                     /gene="hyfC"
                     /locus_tag="OO332_000113"
     CDS             117754..118695
                     /gene="hyfC"
                     /locus_tag="OO332_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5940733.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            118723..120162
                     /gene="hyfD"
                     /locus_tag="OO332_000114"
     CDS             118723..120162
                     /gene="hyfD"
                     /locus_tag="OO332_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5940734.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            120174..120824
                     /gene="hyfE"
                     /locus_tag="OO332_000115"
     CDS             120174..120824
                     /gene="hyfE"
                     /locus_tag="OO332_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5940735.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            120829..122409
                     /gene="hyfF"
                     /locus_tag="OO332_000116"
     CDS             120829..122409
                     /gene="hyfF"
                     /locus_tag="OO332_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5940736.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            122399..124113
                     /gene="hyfG"
                     /locus_tag="OO332_000117"
                     /pseudo
     CDS             122399..124113
                     /gene="hyfG"
                     /locus_tag="OO332_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            124123..124668
                     /gene="hyfH"
                     /locus_tag="OO332_000118"
     CDS             124123..124668
                     /gene="hyfH"
                     /locus_tag="OO332_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5940737.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            124665..125423
                     /gene="hyfI"
                     /locus_tag="OO332_000119"
     CDS             124665..125423
                     /gene="hyfI"
                     /locus_tag="OO332_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5940738.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            125416..125829
                     /gene="hyfJ"
                     /locus_tag="OO332_000120"
     CDS             125416..125829
                     /gene="hyfJ"
                     /locus_tag="OO332_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5940739.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            125859..127871
                     /gene="hyfR"
                     /locus_tag="OO332_000121"
                     /pseudo
     CDS             125859..127871
                     /gene="hyfR"
                     /locus_tag="OO332_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            127893..128741
                     /locus_tag="OO332_000122"
                     /pseudo
     CDS             127893..128741
                     /locus_tag="OO332_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(128779..129840)
                     /locus_tag="OO332_000123"
     CDS             complement(128779..129840)
                     /locus_tag="OO332_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5940740.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            130053..131516
                     /gene="bepA"
                     /locus_tag="OO332_000124"
     CDS             130053..131516
                     /gene="bepA"
                     /locus_tag="OO332_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5940741.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            131537..131896
                     /gene="arsC"
                     /locus_tag="OO332_000125"
     CDS             131537..131896
                     /gene="arsC"
                     /locus_tag="OO332_000125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5940742.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(132029..132775)
                     /gene="hda"
                     /locus_tag="OO332_000126"
     CDS             complement(132029..132775)
                     /gene="hda"
                     /locus_tag="OO332_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5940743.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(132825..134114)
                     /gene="uraA"
                     /locus_tag="OO332_000127"
     CDS             complement(132825..134114)
                     /gene="uraA"
                     /locus_tag="OO332_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5940744.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(134200..134826)
                     /gene="upp"
                     /locus_tag="OO332_000128"
     CDS             complement(134200..134826)
                     /gene="upp"
                     /locus_tag="OO332_000128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5940745.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            135151..136188
                     /gene="purM"
                     /locus_tag="OO332_000129"
     CDS             135151..136188
                     /gene="purM"
                     /locus_tag="OO332_000129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5940746.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            136188..136826
                     /gene="purN"
                     /locus_tag="OO332_000130"
     CDS             136188..136826
                     /gene="purN"
                     /locus_tag="OO332_000130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5940747.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            136998..139064
                     /gene="ppk1"
                     /locus_tag="OO332_000131"
     CDS             136998..139064
                     /gene="ppk1"
                     /locus_tag="OO332_000131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5940748.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            139069..140610
                     /gene="ppx"
                     /locus_tag="OO332_000132"
     CDS             139069..140610
                     /gene="ppx"
                     /locus_tag="OO332_000132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5940749.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(140649..>141661)
                     /gene="pdeF"
                     /locus_tag="OO332_000133"
     CDS             complement(140649..>141661)
                     /gene="pdeF"
                     /locus_tag="OO332_000133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5940750.1"
                     /translation="ESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHI
                     YLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMA
                     QPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAE
                     NRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQA
                     QITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVA
                     SICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRE
                     SA"
BASE COUNT        35372 a        36506 c        34885 g        34898 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaagc cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgcccct gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacaga aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccat atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcgt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taagatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcaatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgga tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaatattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acttgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct agcgcgtctt atcaggccta caaaccggct ctttaacgcg tagggcggat
    35341 aaggcgttta cgccgcatcc gccaatagtg ccagatacga cgctcattca actacaatcc
    35401 cagcgccgtc cccaccaaca accagatatt cagcgccacg accagcacta caatcaccca
    35461 gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc atcaacttgc tgtcactggt
    35521 gaaaatcagc agtggaacca gcgccagggc gataccaaaa cttaacagca cctgactcat
    35581 aaccagaatc cgtgtcggat ctaatcccat cagaatgaca ataaatgacg gcaacatggt
    35641 gactgtacga cgcacccaca gcgggatatg gaagcgaatg aatccctgca tcaccacctg
    35701 ccccgccagt gtccccacca ccgttgagga cagtccggca gcaaccagac ttaacccaaa
    35761 gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc agataagcct catcaagatc
    35821 ggcaacacca gtatgaccag aaaagtggaa cgccgccgca gctgtagcca tcatcgccag
    35881 attgacaaaa ccggcaatcg tcatggcgat agccacatcc catttggtgg cggaataacg
    35941 ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg
    36001 cggcataatc gtcgccccta acacgcctgc tgccaggaag accgcctccg aagtaggtaa
    36061 actcgggatc accattcctt tacccagctg cgccaggtta ggctgggaga aaatcaactc
    36121 gacaatgtaa gccgcggcaa caaacaacag taacccgcca atcactttct ccagcggttt
    36181 ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc
    36241 ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc gccgcaccaa taaattccgc
    36301 caggtcggtt gccatcgcaa taatttctgc ctgaacccaa tagaaccaca ctacgggacg
    36361 cggatagtga tcacgaatct gctccgccag atttttaccg gtggcaatcc ctagtttggc
    36421 agagaggatc tgaattagca tcgccatcag gttggcccaa acgacaaccc acagtagctg
    36481 atagccgaag ctagcacccg cctgaatatt ggtcgcaaag ttaccgggat cgatataacc
    36541 aatcgccgca atgaacgcag gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc
    36601 gctgctactc tcaacgcgat agttcgtcat cttgtgcctc taaaacatag cctttgctat
    36661 gtttcatgct attctaaacg agaatgatta tcaaattcat ttaaatggat tgtggtgatt
    36721 tctctgatag accaagatta cgattacgaa aagattgatg acttgaatgt tatggttatg
    36781 tttaatgtta gcacatttac ataactttca gcttccatac acaacatagc agaaatgtat
    36841 gacagatcac tatttttgaa gcctgtcaca ggacgtcatt atagtgtgtg tcagatctcg
    36901 ttttccttaa ccatgttaca tagaatgtgc acggaaattt aacctgcctc atatttggag
    36961 caaatatgga ccgcgtcctt cattttgtac tggcacttgc cgttgttgcg attctcgcac
    37021 tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta
    37081 tcgaagtgtt actggcgtgg ttcttcctga actccgacgt tggtttaggc ttcgtgaaag
    37141 gcttctccga aatgttcgaa aaactactcg gatttgccaa cgaagggact aacttcgtct
    37201 ttggtagcat gaatgatcaa ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg
    37261 tctttatctc tgcactgatc ggtattctcc agcacattcg cgtgttgccg gtgattatcc
    37321 gcgcaattgg tttcctgctc tccaaagtca acggtatggg caaactggaa tcctttaacg
    37381 ccgtcagctc cctgattctg ggtcagtcag aaaactttat tgcctataaa gatatcctcg
    37441 gcaaaatctc ccgtaatcgt atgtacacca tggctgccac ggcaatgtcc accgtgtcga
    37501 tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg
    37561 tactgaacat gttcagcacc tttatcgtgc tgtcgctgat caatccttac cgtgttgatg
    37621 ccagtgaaga aaacatccag atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc
    37681 tgggtgaata cattctggca ggtttcaaag ttgccattat cgttgccgcg atgctgatcg
    37741 gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc caccgttact ggctggtttg
    37801 gctacagcat ctccttccag ggcatcctgg gctacatctt ctatccgatt gcatgggtga
    37861 tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat catggcgacc aaactggttt
    37921 ccaacgagtt cgttgcgatg atggatctgc agaaaattgc ttccacgctc tctccgcgtg
    37981 ctgaaggcat catctctgtg ttcctggttt ccttcgctaa cttctcttca atcgggatta
    38041 tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc
    38101 tgaaactggt ttacggctct accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg
    38161 tgctgtaaaa ccatacataa aaaagccggg gatcattccc cggctttttt acgcctgtta
    38221 atcagctaat ggctgcgggc gaccaatcaa atacccttgc agatattgca ccccgagctt
    38281 atgcaatagc gcctgctgct gttgcgtctc gacaaactcc gcgaccacac tcaatgactt
    38341 cgctttcgcc agatcggtaa ttgatctcac aatcatcgca tccagcgtat tcgtgacaat
    38401 atctttcaca aagacgccat caattttgat gatatcagcc tgcaaacgct ttaaccgttc
    38461 gtagttggca tatccggtgc caaagtcatc aatcgcaatc cggaaaccaa acttatgcag
    38521 ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac gcctgctcct cggtgatctc
    38581 aagaatgacc gcctgcgggg agatgtgata acgcttaaac agacgaataa tccgtccggc
    38641 aatattcttt tgcagcagcg tgagcggcat taaattgact gaaaagcgcg gtcctttttt
    38701 gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc agcacttgca aatcaaaacg
    38761 cgcgctaagg ttaaactgag caataagggg cagaaactta tccggggtca taatgccgcc
    38821 gtcatatttc agtcgcgcga ggatctcatc ataaccttca ccctctttgt tgcgaattgg
    38881 ctgggcgtag agcagcaaat caccctgatc taacgccgtg cgaatggtat tcagcaatag
    38941 cacctgttta gtggtctgcc cggaaaccat ctcctctctg ctatccagcg ccagcacatg
    39001 atgatgtgcg caggattgct ccgccagcca gcttaactgc cccaacaagg gttgcagggt
    39061 ttcctgattt ccatcaaaac gcccccaggc agcaccatag cccatatcca gcccggtatt
    39121 gttccagtga atttgccgac tattcaggat gttaaccata tgctggagtc gcccttccgt
    39181 ttccggcccg ctcagcacta acagcagttc actacccggc aattgataca acttttcgtt
    39241 ttcctgcatc aacggcagca gcgtacggca aattgagcgg atacagtgaa cgcgcatcat
    39301 taagccgtaa tgacgactca taaactcaag attatcaatg cgcaggcagc aaaaactctt
    39361 gcccgcctct tgctccggcg cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg
    39421 atctgttagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    39481 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcgagaataa atgccagcga
    39541 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    39601 aacagcctag gtgagattta aaaacggata gcgaagatta ccgaccccga gggtaaagat
    39661 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    39721 cagaagtagc agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    39781 gcccaacgac ggagcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    39841 catgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga aaaggcttag
    39901 taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    39961 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    40021 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    40081 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctgccgag
    40141 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    40201 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    40261 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    40321 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    40381 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    40441 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat
    40501 ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtaaact gtattacaaa
    40561 agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga aatttggtgg
    40621 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    40681 ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt ggtggagcta
    40741 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata
    40801 gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc cgcccgaatg
    40861 tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat
    40921 tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta aagtggtgtt
    40981 ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtc caaggagagg
    41041 atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat taataaatgg
    41101 gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg taaagcccga
    41161 ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg tccagaaggt
    41221 cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct ggtgacactg
    41281 gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg
    41341 attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga aaagattacg
    41401 caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac aaactgttaa
    41461 ccgttggatc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg
    41521 tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca cgccagcctt
    41581 ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg ctcctggagc
    41641 acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag acattgagca
    41701 ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg cccgtctcga
    41761 tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg tttatctttt
    41821 tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct ttgttgatac
    41881 gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct ggagactgcc
    41941 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    42001 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg
    42061 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    42121 ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    42181 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    42241 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    42301 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    42361 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    42421 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    42481 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    42541 taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg
    42601 ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt
    42661 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    42721 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    42781 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca acacacggtt
    42841 catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc ggcttctcac
    42901 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    42961 aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    43021 tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    43081 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    43141 caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    43201 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    43261 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    43321 ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt gttttcgttg
    43381 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    43441 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    43501 gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43561 gatcccgtca tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag
    43621 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43681 gatcccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt
    43741 aatcaattgg tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa
    43801 ggcgcttttt tactatctgc gatactcaaa gattcgaacc tgcagcaggt ttgagttgag
    43861 cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa gggcgagcga agcgagtcat
    43921 cctgcacgac ccaccaatgc aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt
    43981 tgtaagattg atttttcttc tcaagttcct tgttcttcta ccgatattac ctcgtacagt
    44041 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
    44101 agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg gataagtcag
    44161 caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg cagtgctgaa
    44221 gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
    44281 gcgcagttgc agaaacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
    44341 aatgtatccc ttctaaatcc tgttgaaaaa acgactcata tcgatatcta tatctgatac
    44401 aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac cacgtcccat
    44461 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
    44521 tcaaccaccg gcgtaatgcg cttaaccacc agtgggctac cttcaggcaa tggtagcgcc
    44581 agtgcaggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
    44641 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
    44701 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
    44761 cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
    44821 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcca
    44881 aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcatgcagg
    44941 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
    45001 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
    45061 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
    45121 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
    45181 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa gccgatacgc
    45241 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
    45301 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
    45361 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
    45421 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
    45481 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
    45541 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
    45601 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
    45661 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
    45721 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
    45781 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
    45841 gctggttggt ctggtgatga acgttcacgg tgcaaggggc agccttgagc aggtcggtaa
    45901 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
    45961 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
    46021 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
    46081 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
    46141 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
    46201 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
    46261 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
    46321 ctgtgcggtg ctagcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
    46381 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
    46441 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
    46501 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcaattaagc gacgcccttt
    46561 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
    46621 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
    46681 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
    46741 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
    46801 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
    46861 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
    46921 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
    46981 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga
    47041 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
    47101 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgcca
    47161 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatctg
    47221 ccggagtatg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
    47281 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
    47341 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
    47401 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
    47461 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
    47521 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
    47581 gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
    47641 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
    47701 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
    47761 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
    47821 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
    47881 gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgcccc
    47941 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
    48001 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
    48061 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
    48121 cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga atggcgcgaa
    48181 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
    48241 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
    48301 agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa
    48361 gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca tggcgtatct
    48421 ggctgaaagc tgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
    48481 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
    48541 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcaa agcgtcgttc
    48601 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
    48661 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
    48721 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catactgcgc
    48781 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
    48841 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
    48901 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
    48961 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
    49021 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
    49081 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
    49141 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
    49201 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt
    49261 ggcgaaactg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggttggtac
    49321 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
    49381 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
    49441 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
    49501 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
    49561 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
    49621 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
    49681 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
    49741 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
    49801 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
    49861 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
    49921 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
    49981 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
    50041 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
    50101 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
    50161 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
    50221 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
    50281 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
    50341 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
    50401 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
    50461 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
    50521 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
    50581 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
    50641 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
    50701 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
    50761 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
    50821 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
    50881 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
    50941 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
    51001 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
    51061 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
    51121 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
    51181 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
    51241 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
    51301 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
    51361 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
    51421 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
    51481 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
    51541 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
    51601 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
    51661 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
    51721 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
    51781 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
    51841 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
    51901 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
    51961 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
    52021 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
    52081 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
    52141 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
    52201 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
    52261 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
    52321 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
    52381 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
    52441 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
    52501 ttgaccggaa aaaaatttct gccgaccagg ttgctcagga agttgaacgt tttctgagcg
    52561 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
    52621 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
    52681 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
    52741 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
    52801 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
    52861 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
    52921 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
    52981 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
    53041 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
    53101 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
    53161 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
    53221 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
    53281 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
    53341 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
    53401 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
    53461 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
    53521 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
    53581 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    53641 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    53701 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    53761 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    53821 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    53881 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    53941 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    54001 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    54061 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    54121 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    54181 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    54241 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    54301 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    54361 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    54421 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    54481 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    54541 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    54601 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    54661 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    54721 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    54781 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    54841 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    54901 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    54961 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    55021 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    55081 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    55141 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    55201 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    55261 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    55321 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    55381 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    55441 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    55501 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    55561 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    55621 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    55681 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    55741 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    55801 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    55861 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    55921 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    55981 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    56041 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    56101 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    56161 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    56221 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    56281 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    56341 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    56401 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    56461 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    56521 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    56581 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    56641 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    56701 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    56761 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactt cttttccaca
    56821 cgaaaattaa aaactacatt tgtcggcagc aacaactgcc gacaaatcag attattgaca
    56881 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    56941 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    57001 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    57061 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    57121 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    57181 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    57241 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    57301 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    57361 aatggtcacc ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt
    57421 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    57481 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    57541 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    57601 tttggtgaca agaatcagtt ccgcaccata agcatgcatc gccgcacggc gttcctggct
    57661 catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag
    57721 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    57781 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    57841 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    57901 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac
    57961 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc
    58021 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    58081 aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat
    58141 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc
    58201 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    58261 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    58321 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccgga ccctgatacg
    58381 caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg
    58441 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    58501 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    58561 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    58621 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    58681 cgccaaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    58741 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    58801 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    58861 cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga
    58921 acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca
    58981 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc
    59041 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag
    59101 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg
    59161 caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc
    59221 cactgcaaca tactctttaa aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg
    59281 gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc
    59341 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca cagcaccaaa ctcgccaatc
    59401 gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg
    59461 acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg
    59521 ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag
    59581 atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc
    59641 cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc
    59701 ggcgatacgg caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta
    59761 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    59821 agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt
    59881 aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc
    59941 aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    60001 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    60061 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    60121 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    60181 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    60241 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    60301 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    60361 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa
    60421 tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa
    60481 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
    60541 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    60601 ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa
    60661 tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg
    60721 tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga
    60781 gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc
    60841 ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt
    60901 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt
    60961 gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc
    61021 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    61081 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    61141 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    61201 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    61261 caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    61321 gtttttcagg aactgggtca taaactgttc ggttttggct tcatcgccac cgtcagcttt
    61381 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    61441 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg
    61501 gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    61561 gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt
    61621 cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt
    61681 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    61741 ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    61801 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    61861 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    61921 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    61981 atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt
    62041 ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca
    62101 ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc
    62161 tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg
    62221 cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga
    62281 tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat
    62341 tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat
    62401 gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga
    62461 ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc
    62521 atacgccgtt tcgccaggat cggccaccat atcaccagtg actgaagaca tcatcacaat
    62581 gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc
    62641 tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc
    62701 cagacgacaa acgcctgcgt tattcactag gatatcaatg cgcccttctt tttccttcgc
    62761 gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca
    62821 gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag
    62881 caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg
    62941 caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt
    63001 gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct
    63061 gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc
    63121 aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg
    63181 ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat
    63241 cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg
    63301 ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc
    63361 cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac
    63421 atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc
    63481 cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc
    63541 gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag
    63601 ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag
    63661 tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc
    63721 cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc
    63781 agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc
    63841 gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag
    63901 aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt
    63961 caggtacaac atcttccact ctcctgggct ggcagcaaaa attccgctta agttcttatg
    64021 acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga
    64081 tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca
    64141 actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc
    64201 gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc ttgccgttga
    64261 tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg
    64321 cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    64381 cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg
    64441 cgaatatgcc attcagcacg cggtggaagg cgctgaagat agccgggaag gtggtgttaa
    64501 cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg
    64561 tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt
    64621 gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc
    64681 gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggca cagcgcagaa
    64741 actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    64801 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    64861 taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg
    64921 caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa
    64981 acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc
    65041 caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa
    65101 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact
    65161 ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca
    65221 ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct
    65281 gctgggcgag gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt
    65341 aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    65401 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    65461 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat
    65521 gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc
    65581 ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg
    65641 ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc
    65701 catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgagggcct gcgcccgcat
    65761 tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat
    65821 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt
    65881 attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt
    65941 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    66001 ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    66061 aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc
    66121 gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct
    66181 gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc
    66241 ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag
    66301 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct
    66361 tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt
    66421 caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc
    66481 tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga
    66541 cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta
    66601 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcacca tcaacttgtc
    66661 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    66721 acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    66781 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    66841 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga
    66901 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    66961 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    67021 cacaaacaca tagctgccgc cggcatccac gccgtctttg ataaccgcca cttcgcggcg
    67081 cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc
    67141 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    67201 cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    67261 atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    67321 tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc
    67381 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    67441 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    67501 gcaatgttct ggcaaaatac cactctgaac ctgagacatt gttcctcctg aaaataagaa
    67561 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt
    67621 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    67681 tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata
    67741 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    67801 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    67861 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    67921 atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc
    67981 gcggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt
    68041 tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac
    68101 gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    68161 ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    68221 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    68281 tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    68341 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    68401 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag
    68461 gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg
    68521 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    68581 ccctgctcgc gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    68641 agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga
    68701 tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    68761 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    68821 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    68881 ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    68941 cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa
    69001 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    69061 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    69121 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    69181 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    69241 caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc
    69301 gatacgatat aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa
    69361 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg
    69421 ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac
    69481 agcaagccga aagcaaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    69541 ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg
    69601 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    69661 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    69721 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    69781 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    69841 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg
    69901 cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc
    69961 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    70021 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    70081 atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    70141 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    70201 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    70261 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    70321 agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt
    70381 ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc
    70441 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta
    70501 gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc
    70561 gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc
    70621 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca
    70681 tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    70741 catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat
    70801 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    70861 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc
    70921 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc
    70981 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    71041 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    71101 gtcagggatt gggtgtaact ccctcccccc cattcccgca tccgctgatg caacgtcaac
    71161 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    71221 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    71281 cggcgtacgg cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata
    71341 gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc
    71401 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    71461 cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc
    71521 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    71581 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    71641 ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc
    71701 ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg
    71761 atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg cgttgctatt
    71821 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    71881 ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat
    71941 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    72001 ggatgcagct gatcgtaaat ctgctgccag gcggtaatcg tcaaagcatg ttcatagaca
    72061 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    72121 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    72181 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    72241 taacgactac tgcgtttacg ttacgtcccg gcagaaaaga gctgttcgag cgcatttctg
    72301 gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat
    72361 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    72421 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    72481 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    72541 caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    72601 cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    72661 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    72721 atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    72781 gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag
    72841 tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac
    72901 gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    72961 ccaagcgtga tcccggcggt tgatgaaaga taagaacctg tacgcgaaac tacatgcgcc
    73021 aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt
    73081 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    73141 agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca
    73201 tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    73261 tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc
    73321 gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    73381 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    73441 agcatacgtt tcgccaaatc cacaatgacg gctgcggctt caaccggcgg cgtgccgccc
    73501 tggtgaatgt tagagataca ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag
    73561 taaacggcgt agcaggagag gctttctgac tgccctaacc ccggacgttc ccccaccagc
    73621 aggatcacca ctttcgcgcc gaggatctca ccgatctgat cttcaatctt cacgcgacca
    73681 taacgaacaa agaatggcgt gccgactttc agcccggcct gtttcaggcc cgccatcagc
    73741 ggcggcagga tctcttcgta gttgacggtg atggcatcag ttgacaggcc atcagaaata
    73801 acgacctgta cgtccggatt agcaacacac tgtgctttca gcgcttcaac agcttctgca
    73861 cacaggcggc ggcccatatc cgggcgagtc aggtagagat ttttgtcgct aatttcagag
    73921 cgtacttcca gcaagccctg cgctttcacc cactcttccg gcacttcttt cagtacggta
    73981 tctttcgaac gagagtgatc ggccaagaaa cgcagcagcg cctgagtacg tggacgcgga
    74041 ccggcacgac cggtacaaac gcgggccacg gtgctgcggc gcagttctgt taatacgtct
    74101 gcgcgatgcg ggttttcaac accaatccac gcttttgctt cagcggaacc taaatccagc
    74161 gcacagcttt ccgaggtcac cggtgccgca cagttggtgg tggcgcactt tgcttctgac
    74221 ggcgcggggg ccgcttgtcc cattgacgcc atcacgctgc gtacaatttc ttcaatctgt
    74281 ttttgatcca tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc
    74341 gtttggtcag gcgaccgttt gccataatgc ccatgctttc cagccagcgt tcaaactctg
    74401 gtgacgggcg caggttgagt aactgacgca cagtggcggt atcgtggaat gcggtggtct
    74461 ggtagttgag catgatgtca tcacccagcg gcatccccat gatgtagttg cagcctgcgg
    74521 tggcgagcag gatcatcagg ttttcgttga ggttctggtc agagtcagcg tggttggtgt
    74581 agcagccgtc acagcccata gagatgccgc tcagcttgcc cataaagtga tcttctaagc
    74641 cagcacggat aatctggcgg tcgttgtaga gatactccgg cccaataaag ccaaccacgg
    74701 tgttgacgat aaacggatcg taatgacgcg ccagcccata gttacgtgct tccatcgtca
    74761 cctggtcagc gccgaagtta gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt
    74821 agaggcagtt ttccccggcg atacgattga actctgcgcc cactgcgcgc gcttcgtcga
    74881 gcatcgccag ttccacgcca aactctttca gccctttttc gctgccacag atactctgga
    74941 aaatcagtcc gccaggagcg ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca
    75001 gtacgcagcc ctgagttggg atgttgaatt tgtcgatcac gccataaatg gtatccagca
    75061 cgcggcttaa gttttccacg tcgtcagtta ccgggttaac gccgatcacc gcatcgccca
    75121 ccccgaagga aagcccttca tagatttgcg cagcgatact ttgcacgtcg tcacgggtat
    75181 cgttcggctg caaacgggcg ctaaaggtgc ccggaatacc gatggtggta ttggcctttt
    75241 tgattaccgg cattttcttc gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta
    75301 tcgccgcgac cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg
    75361 tttcatcgct cagcacatac tcacgcagtt cgctgatgct ccagttttta atctggttgt
    75421 aggccgtttc gttaacgtcg tcctgaatca gccgcgtcac gcagtcatct tcataggcaa
    75481 tcaccggatt attgcggatg tccgctacgg tcatttccga caacacctgc tttgccgcca
    75541 cgcgctcctg tgagcttgcc gctgcaacgc ccgccagcac atcccccgaa cgcagttcgt
    75601 tggctttagc cagcacctct tttacatcct taaactgata tacattgccg aacaatgtgg
    75661 tctttagttt cataagtcgt tccctcagga agaagtgatg agtaacattt ctcacagcct
    75721 gcaaacaatt ctatttcatc gttagccctt gtcatgtaag gtgatttcct taacacgccc
    75781 gttacttatc ctgccgaacg caaccgaact cgccggatac atgagcgggg ttatcgagtc
    75841 gttagatcga ggtatccggt tttgagcaaa acaccactca cagcaaaggc catagacgcc
    75901 gcacaaccaa aggacaagca atacaaactc accgcttcac tgacgcccgg cctgtttttg
    75961 ctggtgcatc ccaacggcag caaatactgg cgattccggt actggataga caagaaagag
    76021 cggttacagg ctattggggt atatccgctg attagcctca aggaagcccg caaacgcgcc
    76081 acagagagcc gtttactgat agcccaggga attgacccaa tggatttggc acgcaaggag
    76141 aaagccattg atgcactcaa catggcggca ggttttaaga cggtagcgga ggactggctg
    76201 gctaccaggg taggcggctg gagtgagagc tatacaaagc aggtcaaatc agcactggag
    76261 aaagacgttt atccggtact tggcaagcgt tcaatcgttg atataaccgc ccgtgacgtg
    76321 ctggcattac tccagaagaa agaacgtacc gcaccggagc aggcccgcaa gctacgccag
    76381 cgcatcggtg agatcttcaa gtttgccgtt atcaccgaac tggtaacgag gaatccggtt
    76441 gcagatcttg atacggcact gaaggcccgc cgacctggtc acaacgcgtg gataccgatt
    76501 agtgaaattc cggccttcta caaagccctt gagagggccg ggagcgtcca gattcaggcg
    76561 gcaatacgtt tgcttatatt aacggcatta aggaccgcag aactgcgttt gtgccgttgg
    76621 gaatggataa acctggagga cgcaacaatc acattgcccg ctgaagtcat gaaggcccgc
    76681 cgcccgcatg tggtcccgct atcacggcag gcggtcgagc tattgcagga ccagttcaca
    76741 cgcagcggat acagtgtttt cgtgttcccg ggccgattca tggataagcc attatcagcc
    76801 agcgcgatac tcaaggcgct tgagcgtatc ggatacaagt caatagccac aggccacggc
    76861 tggcgcacaa cgttcagcac cgcacttaac gaaagcggca ggtataaccc tgattggatt
    76921 gagatccaac tagcccacgt cccgaaggga attaggggag tttacaacca agcggcctat
    76981 ctcaaaatgc gccgtcaaat gcttcaggac tacgccgacg cgattgattc gatactggct
    77041 gatggtatca atccgcttga actgtaatga atgattaatc acgttttccg acgactccac
    77101 cggagtaggc cggataaaca ctggacacct tcaaaataac tattcacttt ttccggattc
    77161 gtccggcttt ttttgtcttt gccgatggga tcaattattc caattattcc agtatcgttg
    77221 gaataaatga ataaatatca atgacttaaa gccgtgtttg caacagggtg gatcggggat
    77281 cgaaaatagg atcaacacaa aaacaccctg gaggctacag cacactaggg ggacacaaaa
    77341 acaacacagg aaaaaaatac catgtaccaa tcagcaaccg cctacgtggc gaaaccaggc
    77401 gaaacactgg tcaaacacaa aattgcaaca ctccaagcac ttcaaacact ccaagccctt
    77461 ccggaacagg ttcaggccgc ctttcgtgat ggcatgaaca tcatccgctt cgatgggtat
    77521 cgctgggaat acaagccagc aactggattc gtgcgcatag atcaaatctt gtcgccagcc
    77581 aatgaagagc cagcgctagc caatgcagaa ccagcgcagg agcaggagca ggagcaggag
    77641 caggagcagg aacaggaaca ggaacaggaa caggaacagg aacaggaaca ggaacagaat
    77701 aaagcggcgg ctattgccga gttcaaggag ctgcaaagac aacaaatcac agcgcagcaa
    77761 gaagaatgcc gccacccgct ggcaatgccc aagccagtag cgaaagccaa cacagacaag
    77821 ccgacttcat ggtttaacga agaagcggaa gaagatttgc gttttgatag taaagaggag
    77881 cagttgcctg aagagcggga acaggtggaa acggaagacc agcaaaacga ttatcgcaac
    77941 cttccagccg tcaataacgc tatggcttac ctcttcaaaa gcgggctgtt tcacaagctg
    78001 aaaggcgatg agttaaagct atatacgtgg atggtatcca agtgcaggta tcgcggtaac
    78061 gacaaatatg agatttggta ttcccgcacc aaaatggcaa aagcgttagg taagagcgtt
    78121 gacacgatag atcgccacct ggaagcgttg agaaagctgg gacttatcca ggaaatgcgc
    78181 caggcactga ggggcaaatg tggaaaaacc tacagactgc gaatcccgtc aaaaggcaca
    78241 gcagaagagg cctcaatgac ggcacagcag cacgaaagag aagcaaaagt cagctacctg
    78301 gtacactagg cagcgggtca gcaaaggggg gcaaatgccc cttttttcat gtcagataat
    78361 cagatcatta cctcaataaa ctaataatca catcgcggtt acaaccagtt tgatagtgat
    78421 agctcaaacc cagacgtggc gctggctgca agcaaaataa gccgcaaaaa tgcggtccta
    78481 tttgccagaa ccttttatta ttcaattggt tagtgcaaga aaaagcgtga ttactgttca
    78541 agaagcatcc agaaaggtca tgattttgcc atgattttga cgctgaaaaa aaataggtgg
    78601 gcatttttgc ccactcacta gccgcaaatt tgcccactca ctagccgcat ttttgcggcc
    78661 caaagtaata aatacagaga aataataaat acagatcagc gccaagattt ttcgtctttt
    78721 cggcggaatc atgagactag tgcttacgtc ctgataacct ccagagcaga ccttaacttc
    78781 tgtgcagcag gactacgctc attgccgctc ggctaggtca aatcctcacg ataaagctgt
    78841 tcggattgtg accataatgt taaaggactc tttaaatcat tccttgacga attaactagc
    78901 tggcttaagc ggaatagcat gcaggataaa aacaaaacca gcttagcagt ggggagagct
    78961 tcgcttcgct cgtctcctgg ggccaatgct caatgaaacg acgtaaggca cggagcgctc
    79021 accataggta gacacataaa catctgagag cgcaccttct atcgcgctac gcgctcaata
    79081 aaaaccaacg acaccgccac actgcaagac caatggtttt tatcgcgtat acagaccaga
    79141 caggaaacag cagcaaagca gcgtatagcc aggtgcagac agcacactca cacgacgcac
    79201 agcaggctta agtgatcact caggaaactt tatccccgcc gccgatcacc gcgaactatc
    79261 atgaccgcgc caaattcgaa aggaatggcc catagaacgc gatgaacaca cataagcgct
    79321 aacttgctta cctgaaaact taacgtcgcc acagagcgat tgagagcgct taaaatcgat
    79381 gcgatttcat taaatttttc tcactcgcct gaatggtggc gatcaggact gatcgcaaca
    79441 ttgccccgcc tcgatattga gcgcataata gatacataca ttaagcagaa aagagataag
    79501 tttattgtga aaatcagggg gttacacatc atgcgcatat cagaacaaca acggcagatc
    79561 ctcgactatc tggcaaaaaa cgaatcaatc accgcacgac aagccgcaga tctcgtttat
    79621 ggtggcaatg ttacccgcgt ccaggttgac gccgcccgcc gctcactcat gacgatggtt
    79681 agtaatgggc taatcaggaa acaaggccgc gctttcgtag ctgaaagaca tatcaatcac
    79741 gcttatagcc aggacgttga agaattgcgc cgccagatcc tttgggtatt cagcaaacag
    79801 ggtcaggagt atttcctttc tgaacaccca aatttcgccg ccaaagagtt aacagtaagc
    79861 ggattgtcgt gcgcactgga ttatcttgtc ggtgaaggtt gcgttactta caagcagcta
    79921 tggcacgccc tgaatacgct tgtagaagat ggtttactca ttgccacgcg tgagaacatc
    79981 gaatactgga taatgccgcg ttcaggtcgc cgcaatcatg cacggtatat ttcaggcaac
    80041 agatacagaa cgccggactt taatccgaca caggaagatc gggaccgtgc cgaaatgatg
    80101 tgcagggaat aggagaaaag aattatggat aagttatttt cgatgcagtg aacacaagcg
    80161 ggcgcaatgc ccgtttttat tgccttattc cacgcctgac gaatcaattt gatcacacct
    80221 gagcaaatgc atagccagaa gttaaggccg ttatattgcg attgggagcg ttttaaacgg
    80281 tatcaattta attaattctg atgtggtgat ttgcaggcaa aaaaaacccg ccggagaagc
    80341 gggaaataga gacatatcgt tgtagttatt aacagatcac tttgaggtcg agggcggcta
    80401 accctcgcta atattatccc gccattacct tgcgggtgca aataatcttt tattgctttg
    80461 atgatttaat aaatagcact ttttgctaac aaaaaccgct gtcaatatat ggccaaaaat
    80521 gtcaacagaa aaaataaggt ttataatatt aagtatcaca tgggtaatgg gttcagggaa
    80581 tcggaatcca ccgcctcacg gaaattcaaa atttgcttct tctttcctcc gtggggcggc
    80641 ttctttttcg tatcgaggta tttatgaaag caataacatt agaacagttc gcagcaatgg
    80701 cgaatgtatc gaccagaacg ctgaggcgct ggctaaaaaa accatcaacc ggagtccctt
    80761 tgccatttac cccacgcggt actcgccaat tattctggga gcacgaatgc aaagaatggt
    80821 tgtacagaac agaacgataa taatcacgca tcacattttg aggtaaacat catgaccaat
    80881 atcgaaagat tagaacgctg tgccgatatt atgcggcgca gatggattta tgaccctgaa
    80941 gcgggtttat tgtttagccg tgaaacaaaa gacgttatca aaggcagcat gaccaataaa
    81001 gggcatttgg ttgtaacggt acatgaaggt aaattcaaag tgacactcac ctatcagaaa
    81061 gcctgctatg tatacacata cggcgcttat gacgagacat tatatgaagt gcaccacgtt
    81121 aatttggaca aacaggataa ccgcatcaag aatattcgcc tgatgccgaa agaaaagcac
    81181 cgccggatac ataaaaacgt ccgcaaactt attcggctgg gccacacaca atggcttcaa
    81241 ccgcaattaa ttgaacaatc actataaaca cagtagtaaa taccatgcat tcattagaca
    81301 gttattttca gcgcacgaca gcccctaaat cagccgccca ggaacgccgt gaggaattcc
    81361 atgagaaagt gatgcgttct gctgattata ttgcggacaa gttcgtggaa acggttcgtc
    81421 cacttgttga tgaagtagct gataaacttc agtctgaaat gccggaagat atggaaggca
    81481 ctgcaaagcg gcgactcatt tgctaattaa gtcgtcgttt tggcgtgtca ataagcgctt
    81541 ttaaataatc gaaaaaaggg gggaaagata atggacgcat ttgaggtact ggcaaataaa
    81601 ggcgctgaac tggtagcaca gcgtgataaa accgccaacg aaggggaacg cgcactgcta
    81661 aataaacaga ttaaagcgat ccgtatggcc cagtttaaat taatcagtaa tgaagttatc
    81721 gaaacaatta aaccgcagat cccacaaatc gttgctgacg ctattaaagc cgccggactg
    81781 gagaaacgca ttgccgggct aaaaacgaac gcagagaaaa tggagatgca caaacagagc
    81841 ggtgctccga atcccgatga gtacttctca ccggatgttg agtttatggc gcaggttgag
    81901 gaacgtctgg gtagctgtct caccgaggaa cagcgtcgct attttgatgg agtggatagt
    81961 tcggcaggta ttgacctgaa ctcgtatttc ggtcgtgaaa ttgagcattt tgatgccgcc
    82021 ccgtctatgc cggagccaga accagaaatg gccaccgatc ctgaagctga gcaacggaaa
    82081 cgcgtattca atgcgatagg gattcggtac tgatcggata aaagaaagcc gggaattacc
    82141 ccggctgact taacaatgtg accaatgaac aaatcggaca agacaaacag gataagcagt
    82201 ctcgaaacaa tgttcgggaa ctatattagc acaagtccgg agaaaatcat catgttggca
    82261 ctacgcatca tgcagggcat cgctaagacc ctcgcagaac atgtcttaga cctgaaacat
    82321 tcgccactga gcaaacaggc catgaagcgc taaacgcttc gtctgtgggc tgaatattcg
    82381 cttggaacta tcaataaaat catcgacatg aaatcggggc cgagcaacca gagcgcggag
    82441 gaaatggagt tcatcaagcg tttaattctc attcgcagag acattcatag ccagttacac
    82501 agcgtgggaa tcgatattaa tgatgggacg ggcgattaat tttcggacaa gtactatgcg
    82561 cgcgcgagga gctgaacatg aagcaagata ttgcagacag gctggagata ctcgaaggcc
    82621 agcgggcaga agcgaaacaa ctacgtaagc aggccagaag agcgcacagg aacaacgaag
    82681 cagagttact aaccaaatac atcagtttca ccaattactg catctacgag tgttacaagg
    82741 aagatgcaga ggactggctg gattcattgc cagaacagta ctgagcagaa taacaccacg
    82801 tcatgttgtt ctgaggtgag catatcacct ctgttcaggt ggccaaattt agtgtgccac
    82861 ttcagcatca ccgttgaaat cacaataacg atttttttgt aaatatgctc tcacatatgt
    82921 aggaatagac caggttggca ttgaaacaca tctaccatga ccataataaa gtaatttttt
    82981 gatatgttta tggttttata ttaattaaaa tatatctaaa attgatagtt ttaaaattgc
    83041 aaaatccaga ttgtgtttgt aatgtttaat ctgattgtat tgagtggcgt ttattatgaa
    83101 aaataaatca ggtaattctt gttatagaga agcagtcgat attcatagct cacttgagct
    83161 ttcatttata ttgcataaat atcatttgcg tgtagatgag aagaaatctc gaaatgtatt
    83221 cagcgccata cgtgatgagc tcatccttga tggggattca cgacaaaact tagctacttt
    83281 ctgtcaaaca tggcttgatg atgaaatccg tgagttgctt aatttagcta ttgataaaaa
    83341 catgatttat aaagatgaat atccacaaat ggcggaacct gaaggtcgct gtgtgcgtat
    83401 gctttccgat tcgtggagtt tccccgattc gcgacacacc ttaggttgct caaccattgg
    83461 ttcatctgaa gctgctatgc tcggccgctt ggcgttaaaa tggcagtggt gtaaaaaacg
    83521 cgaagtacaa ggtaaatcaa ccgaacctga tttgcgatcc tgtgcaaata tgctggcata
    83581 aattctgccg ttactgtgat gtgaaattgt gtgaaattcc acttgatgat catcacctga
    83641 taatgaatgc taaagaggtt ttgaagcatg tcgatgaaaa cactattggt gtcgttacga
    83701 ctttaggtat tacttatttt tgtaaagcag attgcgttag tgtttttaag ataagtgtta
    83761 aagttaatat tagtttttat atttacatcg taattatatg tttctatttt aaaaatattc
    83821 gagagtataa aaatgtctaa tgctaatatc gcacccaaaa gtaagaaaat gacagtgggg
    83881 acactcgcta tcatgaatat aactgctgta gtaagtctac gatttatacc ttctgaagca
    83941 gaatacggct taggtgccat tttttactat acctttgctg caattatgtt tttagtacct
    84001 gttgcctttg ttgcggctga gctagctaca acatatccac aaaaaggggg ggcatttcga
    84061 tggataggag aagcatttgg ggctcgctgg ggttttgtag gaatgcttat gacctggcta
    84121 caagtcatcc cctactttcc aacagtactg acatttggtg cagtctctgt tgcatttatt
    84181 gatgttgata tgggggtggc agaatcaatt gccagtaata aatggtatat tttatttttc
    84241 gtactatttg tttactgggg agctatttgt gttgcattac gtggcgttgg tattttctcg
    84301 caggtatcta agtggtgcgg tattataggg acaattattc cagctattat ccttgttatt
    84361 cttggtttct cctatctgtg gttcagcggt aaaccagcgg tgatcccatt aggttggggt
    84421 gatcttttcc ctaatatagc taacttccag aatattgttc ttgccgccag catatttctt
    84481 gcttatgcag ggatggaaat gaatgcggtc catgttaacg atttagataa tccaactaaa
    84541 aaatacccaa ttgcaattac catcgcttct ataggtacta ttgcaatatt cctgctcagt
    84601 actttagggg tcgcctttat tattcctaca gataaaatta gtctaactca aagtttgttg
    84661 gttgcctacg atatgctgtt cgaatgggct ggagtgccat ggcttggctc tgtgatggca
    84721 tttatgctag caattggcgt actgggtggg gtggttactt ggattgcagg gcctaataca
    84781 ggtgtattag cgatcgcgaa agctggttat ttacctaagt ttttccaaaa aaccaatcgt
    84841 catggaatgg ggcaccatct aatgtttgta caaggtataa ttgtctcagt tctttctgtt
    84901 acttttgtga taatgccttc agttcaagca gcttttcaga ttttatcaca gttaacagtt
    84961 atgctctatt tagttatgta tatgctcatg ttcgctagtg ctatctatct acgacattct
    85021 caacctgata caaaacgccc atatcgagcc cctgctttat ttttctgggc aacacttgga
    85081 ttcttagggt cttttctggc ttttgtatta agttttattc cgcccgagca aatcccaact
    85141 ggtagtccaa ccagctatgt tctttatttg ttagtattag ttgccctatt tttattaatt
    85201 ccaatattaa tttacgcctt ccgaaaatct gactggaaag atccaaactc tgatttcgaa
    85261 ccattttctt gggaaaaaag aactagtaat taaattttcc ataagctaca aaagatgagg
    85321 ttaaatatgt ttagtgctga atctccaatt ggtgtaatag actcaggtgt aggtggattg
    85381 acatcggtaa aggaaataat taatttgctt cctggtgaag atattatcta ttgtggagat
    85441 aatatgaatg caccttatgg caaccgttct gccgatgata tcatcagttt gactaaaaaa
    85501 atgctgactt ttttacagtc aaggaatgtc aaactagttg ctgtcgcatg taatactata
    85561 tcctcaactt tggaatccga aaaatacagc ggatatgcta agagctttcc gtttcctatc
    85621 ttatccatca ttgaacctgc agtagaagat gtgattcgcc agcaatataa aaatgttggt
    85681 attatcgcta ccgagtttac tattaaaacg gggtgtcaca aagagctaat aaaaaaattg
    85741 aattcaacaa taaatgtatt tggagaacca agcaaaaatc ttgcaatgtt aattgaggaa
    85801 ggtaatctta atgctcctgc aatcttgaat gacatcaaaa aacatgttaa ccatttaatt
    85861 tccctccatc cagtaaatga aattattctc ggctgtactc actatccaat tgttcagaat
    85921 ttttttgaag cagttgcacc ggatataaaa tttattaatc ctgcacatga tcaggctatc
    85981 tctattaaaa atcacttggg acagctaaat ttattaaact cctcaaatat aggaactttg
    86041 caaataaata ctagtggaag tatggagatc tacaaaacgg tactagggga attgagcatt
    86101 acgaaacctc atacattcag tatcaggcag ttttaaattt atccggtgca ccgtaaaatc
    86161 ccgtccttct gaactgcacc ccaaaagttg gacaccaact gagtaaggtg cattttatgg
    86221 acaaacccag ttacatactg ggtagtgcat ccaattagta gaacatgtgt ttttcgataa
    86281 acgctccgat cactttttcg tggatctcaa ctgagcgtga gaagcagatt gttttacgac
    86341 ccaaccgctt aatgcgggtg cgtagcgtca gattattacg ctcaatgcgt tgggtgaata
    86401 ttttgccggt cagatgctta ttcttcggca cctcccggcc atagctgccc cagtcatcgc
    86461 tggtcagcat gccgatgttg aagggtggca gtgccagtag ctcccggcac gtttgatcgg
    86521 ttcggggacc aaaagtgtag gccagtacac cgcctgtttt ggtgttgtac gcgtacccga
    86581 gccaatgttg ccgggcttta ctgccaacgt agctccattg ctcatcaagc tcgcagataa
    86641 acgccacatc agcatgggca acaggcgaag aagttattcg ctttggcgcg agttttttaa
    86701 agtccggatg acggtgttac taccaatttt cagtgtcctg gcggtatcgc gaaccccggc
    86761 accattaaag gccatttcag ttatcagctc tttcataccc ggcttacgtg cttgataagt
    86821 ataagtgaac tgaaacacac ggtggcagtc acggcagcga agtctgtcac ggcctttagg
    86881 gttctgacca tggcggtaaa cctgagctga ctgacaacgg ggacaatgaa tattaacgct
    86941 ggtcatgaga taacctcaaa agcccgtatt atacatcaga ttcaactaat tagaggcatc
    87001 acccatccag cattataaaa catactaaca ataactaaat tataataatc acaacttaca
    87061 atcaaataat aaatgcataa gattaacaat gattttttcg ggttatattt atctaaaaaa
    87121 gcagaatatt atgtaactta ttattttcta agcgaaaatt tattgtgcag tcagactagc
    87181 aaacttgcgt accacccgaa gaacatgcga agatttaagt attctttctt ttttggtttt
    87241 tatttttcat tttctctttt cttctctttc cggttctctt ctgttctggt tttggttttt
    87301 tcctttttat ttttattctt tcttcagggt tatgctaaaa caccaaaaca tttttcacca
    87361 caaaagcatc caatcatctt gccagaccaa tcaccttaac cggatacact gccggatata
    87421 atcccactaa cacccagcca ccaagcacca tacctgataa ctaccgtatt caggaaggaa
    87481 atgcgagtga tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt
    87541 cgatatagtc ccccgcgcgg acaatcactt cgtcaatgac tgccaacggg agttgttgta
    87601 gctgtgggcg caacaacatg cccagagctt taccaaagtc ctgcccggcc accaccagca
    87661 ggggatgcgg attcggaaaa cgcgcgacga aatcgaccag cgcgttgatg accgtcagta
    87721 ccgcggcgta acgcacaggc agcgaggcgg gaagcgccag cacgtacacg tcagttttgg
    87781 gatcaagatc cagctgaagc agcgcctgtt gccaggcact caccagatcc gtttcatcaa
    87841 tcgggatcgc caccggcaaa ttgcgcagcg gcagttgtac gccctccagc cagattgtgc
    87901 tgccagagag cgaaagggta tgtgcacccg cgccaatcac cgtggcgcat acggtttgcg
    87961 ccggaaactg cacattcatc tcacgcaggc gcggatggtc atgcagcgcc gttgccagaa
    88021 gtgggccaat atcggcaaaa cagaatgggt cggcgggctg gtggcgataa cattcgccca
    88081 cacctccaga aagcgtaatg atttcgggcg taacacctgc gggcagcaaa ccggtttgca
    88141 tcaatgcctg cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc agcgccgcca
    88201 tccgccgggt aacctgcacc agctgcgcgc cggtcagcga acgggcgtca gtgcctgcac
    88261 cgaagcactc atccacaatc atctgccccg gtttatgagc gtaaaccacg cgcccctggc
    88321 tgtcggtttc cagcaggcga ccaccgacgt tgagacaggc agtgccgctg atttttccgg
    88381 catcgaacag ggcgtagttc gcggtgccac cgccgatatc gatattcagt acccgacaca
    88441 gccgttgttc agaaagggtt tgcgccccgg ctccgtgacc ggcgatcacg gattcgaggt
    88501 gcggcccggc gctggcgacg acaaaatcgc ccagcgactg tttttccttc atcacttttt
    88561 ccatcagctg tgcgaccagg ctttcggtaa actggccttc cggcagctgg gcaatgatgg
    88621 tttcgcggat gcgctggctt tcggttttgt cggctggcac gctggcgggc acggacgccg
    88681 ttaccgggat cgattcatca cattcggtga tggtaaaacc cagtagatcc gacacttcac
    88741 gggcttccgg ggtaataatg ctggcgcgca gaaccacgga cattgcctgt tcgccgcgcg
    88801 cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt cacctgcttc ctcctgtttg
    88861 gtgagagatg ccagataatc caccagttgc tgcacgcttt gcgggtcgtg gctgtttagc
    88921 tcgaaaatcg gctcttcgaa acccgtctca agcaataact ttcgcgttgc ggcgacgtca
    88981 gcatctggca tgtccgtttt actgatgacg gcgatttgcc gtttgctgac gccaatatcc
    89041 agcaacccgg caggcaaccg actttccggg tcatttgcgc catggacata gatcagcatg
    89101 tctacatcct gtagcgtggt aattaaggcg tgataccagc ggggatggct aaaatattca
    89161 cccggcgtat caatatcgcc cttatcatta aattccacgg cctgtgtttt tctggcgagg
    89221 gtataattcc cctgcaacgc attaaataac gttgtttttc ctgccccgac gctacccaca
    89281 aacgcaattc gtttcatgaa tgcctcgtat taacttttgg tcatttcaca aagcgtataa
    89341 tttaataagc gacctaaacc gctgacggtt tgtgataacg cttcttctac tgcgcccacc
    89401 gagccataaa tcaccaatgc accgctgaag cggtcgagaa aaccgatatg cacatcagcg
    89461 gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc cgggagttag cgtcataatg
    89521 ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca gttcctcacc gggatgcgcg
    89581 atgagatgcg ccagtgtgac ctgtttgccg ggcacaaatt cctgaatgat gcgttcttta
    89641 tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc gtcagagtaa
    89701 ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcactaagaa tggagagata
    89761 tttactgtta tgaataaact ttcatatgcg acgcatgaat ataaataaaa taatatcgag
    89821 atattttata tactgatttt tttattattc gcaaactcag taataagctt tgctaatata
    89881 tttcaggaaa accgcttacc ctgaatactc agggtaagcg tgagagttaa gaatgaatta
    89941 cagcggttgg gtttgcgctt caaccacggc cagcgccacc atattgacga tacgacgcac
    90001 cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg cccatcagca ccggaccgac
    90061 agtcacacct tccgagctgg aaacgcgcag taagttgtaa ctaatgcggg cagcttccat
    90121 gttcggcatc accagaatat tggcagaacc tttcaaaggg ctgtccggca tacggtcgtt
    90181 gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca tcagatctgg
    90241 cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg ggcagtcaga
    90301 agaaccaaag ttggagtgcg acaacagagc aacgcgcggc tcaataccaa aacgacggac
    90361 agtttctgcc gccatcaagg tgatctccgc cagctcttct gcgtctggtt cgtcattgac
    90421 gtaggtatcg gcaacaaagg tgttaccact cggcagcagc agcgcgttca ttgcacctgc
    90481 ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acgctaaaat gttcatgata
    90541 atcgcccacc gtaccgcaga tcattgcatc ggcttcccca cgctgaacca tgatcgcgcc
    90601 gatcactgtc gggttactga tcagcgcacg ctgcgcctgt tcctgagtga caccgcgacg
    90661 cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg attcgttatt
    90721 gacgatttca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa tgcgcatttc
    90781 gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta ccagttcctg
    90841 agtcgcatgc agaacgcgcg cctcttcccc ttccggcaga actacgcgct tcggcgcttt
    90901 gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga actcagtcag
    90961 cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc gccacgcccg actccatcgc
    91021 cgcttttgcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg gttttggaat
    91081 gatgtattcc ggaccaaagc tcagatcctg aacgccatac gctgaagcca ccacttcact
    91141 ctgttctgca tgggccagtt ctgcaatcgc gcgtaccgcc gccagtttca tctcttcgtt
    91201 gatggcggtt gcgccaacgt caagcgcgcc acggaagatg aacgggaagc aaagaacgtt
    91261 gttcacctgg ttcgggtagt cggaacggcc ggtacagatg atggcatccg gacgcacttc
    91321 tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga tcattggtgc
    91381 ccgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac agcccaggaa
    91441 aatatccgca ccttcaatca catcatcgag ggtacgtttg ccgtcatcca ccaccgcata
    91501 tgcggctttg gtttccgcca tatttggctc acgcccctga tagataacgc cttttgaatc
    91561 gcaaaccacg atgttatgtt tttgcagccc cagcgctacc agcaggttca tacaggcgat
    91621 tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt tctccaccac
    91681 gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc gtgccgtgct gatcgtcgtg
    91741 gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac attctggcgc
    91801 tttaatgtct tcgaggttga tgccgccgaa ggtcggttcg agcgcggcga cgacttcaat
    91861 aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat acatcaatcc cagcgaattt
    91921 cttaaataga acgcccttgc cttccatcac cggtttgcct gccagcgcgc caatgttgcc
    91981 taaccccagc accgccgtac cgttagagat caccgcaacc aggttaccgc gcgcggtata
    92041 tttgtaggct tttagcgggt ctttttcgat ttcaagacaa ggtgcggcaa cgcctggtga
    92101 gtaggccagc gccagatcgc gctgcgttgc cagaggcttg gttggagaaa cctggatttt
    92161 ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt catccatttg
    92221 gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc ctgcccacaa
    92281 cgtggcgcac attattaccc tgccggagtc tacagacttt gagcaagtcc aaactctcac
    92341 cattaatata atgttttggt aacaatccta taacactgat attacctgct taatccagca
    92401 ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc agaagtgtga
    92461 attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa cgagttagac
    92521 ggcatcaaac agttcaccac tgtcgtggca gacagcggcg atattgagtc cattcgccat
    92581 tatcatcccc tggatgccac caccaatcct tcgctgttac tcaaggctgc cggattatca
    92641 caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg caagacccag
    92701 gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca atttcggtgc tgaaattctc
    92761 aaaatcgtac ccggttgcgt gtcaacagaa gttgatgcac gcctctcttt tgataaagaa
    92821 aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg cgttgagaaa
    92881 tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa ttcgcgcggc cgaagagctg
    92941 gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttgccca ggcgcgggcc
    93001 tgtgcggaag caggcgtttt tctgatttcg ccgtttgtag ggcgtattta tgactggtat
    93061 caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt taaatcggtg
    93121 cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat gggcgcgagc
    93181 ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac tatcgcaccg
    93241 aatttgctga aggagctgca ggaaaaagtt tctccagtgg tacgtaaatt aatcccgcct
    93301 tctcagacgt tcccacgccc agcacccatg agcgaagcgg agttccgttg ggagcacaat
    93361 caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc tgttgatcaa
    93421 cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt gttatatgtc
    93481 ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgcggtac aaaaagccaa
    93541 ttctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc tgtggaacga
    93601 ttttcttaaa cataatccta ccgacccaac ctggtatgat cgcgaccgct ttattctttc
    93661 caacggtcac gcgtcgatgc tgctctacag tttgctacat ctgaccggtt acgacctgcc
    93721 gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa accccaggcc acccggagat
    93781 tggctatacg ccaggcgttg aaaccaccac cggcccgctt ggacaaggtt tggcgaacgc
    93841 cgtcgggctg gcgatagcag agcgtacact ggcggcgcag tttaaccagc cagaccatga
    93901 gatcgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg aaggtatttc
    93961 ccacgaagtc tgttcgctgg caggcacgct gggactaggc aagctgattg gtttttacga
    94021 tcacaacggt atttccatcg acggtgaaac agaaggctgg tttaccgacg atacggcaaa
    94081 acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg atccgcaggc
    94141 ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc tgattatctg
    94201 ccgtacggtg attggctttg gttcgccgaa taaagcaggt aaggaagagg cgcacggcgc
    94261 accactgggg gaagaagaag tggcgctggc acggcaaaaa ctgggctggc accatccgcc
    94321 atttgagatc cctaaagaga tttatcacgc ctgggatgcc cgtgaaaaag gcgaaaaagc
    94381 gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc aactggcaga
    94441 agagtttacc cgacggatga gcggtggttt accgaaggac tgggagaaaa cgactcagaa
    94501 atatatcaat gagttacagg caaatccggc gaaaatcgct acccgtaagg cttcgcaaaa
    94561 tacgcttaac gcttacgggc cgatgctgcc tgagttgctc ggcggttcgg cggatctggc
    94621 tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg aaggaagatc cggcgggcaa
    94681 ctacattcac tacggggtgc gtgaatttgg catgaccgct atcgccaacg gcatcgcgca
    94741 ccacggcggc tttgtgccgt ataccgcgac gttcctgatg tttgttgaat acgcccgtaa
    94801 cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt atggtttata cccacgactc
    94861 aattggcctg ggcgaagatg gtccgacgca ccaggctgtt gagcaactgg ccagcctgcg
    94921 cttaacgcca aatttcagca cctggcgacc gtgcgatcag gtggaagcgg cggtgggctg
    94981 gaagctggcg gttgagcgcc acaacggacc gacggcactg atcctctcaa ggcagaatct
    95041 ggcccaggtg gaacgtacgc cggatcaggt taaagagatt gctcgtggcg gttatgtgct
    95101 gaaagacagc ggcggtaagc cagatattat tctgattgcc accggttcag agatggaaat
    95161 taccctgcaa gcggcagaga aattagcagg agaaggtcgc aatgtacgcg tagtttccct
    95221 gccctcgacc gatattttcg acgcccagga tgaggaatat cgggagtcgg tgttgccttc
    95281 taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc gattactggt acaagtatgt
    95341 tggtctgaaa ggggcaattg tcgggatgac gggttacggg gaatctgctc cggcggataa
    95401 gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct
    95461 gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgta tgttcccctc accctaaccc
    95521 tctccccaga agggcgaggg gacggtccgt gcgcttttta gggccgcatc tgcgattttg
    95581 tttaacgggt gatcacaacg tgggcctggc gtctggccca tgccagttat cgcaggtagg
    95641 ttctgcggcg taacgcacca ggcgaaaact ctgaccgtca aagcgccagc gcgcctgaat
    95701 gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc
    95761 aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgttgttga acggcaaacg
    95821 caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg ccaaatcaat
    95881 ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat cggtcagcgc
    95941 agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat tcatccgcca
    96001 gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt
    96061 aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg gttcgtcccc
    96121 tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa
    96181 cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt
    96241 cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa
    96301 ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat ctaatagcag
    96361 ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc gctcaatacg
    96421 taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca gtccattatg
    96481 atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct gccagtcgga
    96541 aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa
    96601 gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac
    96661 aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat
    96721 aataaacaac gaaatattat tgctattttt cagtccatta aatgtgacat ttgcaaatag
    96781 ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc
    96841 tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc accggcgtta
    96901 gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcca
    96961 ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca
    97021 atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt
    97081 tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc
    97141 agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg
    97201 taaacttcac gtttatggcg gataacttca ccatctttgc gcgtgagatc gtaagtaatg
    97261 ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc
    97321 gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag tcgggtttgt
    97381 ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg
    97441 tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc
    97501 gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc
    97561 gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg
    97621 tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt cactgccctc
    97681 aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa
    97741 acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct ggacgttaaa
    97801 ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt
    97861 agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg
    97921 cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt
    97981 gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcaatgat
    98041 gaacggcagc gcgtcataaa cgcccggcgt atcttcattg ggtaaacccg ctttcatgga
    98101 acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga
    98161 gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc
    98221 ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa
    98281 gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac
    98341 atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct tgtccacttt
    98401 ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatatagcg
    98461 ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg caccttcaca
    98521 caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga
    98581 aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggaatatg
    98641 gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa ggcagcgcga
    98701 ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa aaccggtttt
    98761 gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc
    98821 gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg gttgatgctc
    98881 ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga cgtcagtgac
    98941 cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt cgcgccctgc
    99001 acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac
    99061 gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg
    99121 ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc
    99181 gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat
    99241 aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc
    99301 catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat
    99361 aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt
    99421 tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat aagtaactat
    99481 ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc gtttcagcga
    99541 actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gccccgtctc
    99601 ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat
    99661 cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc
    99721 gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa
    99781 tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca acctcaacgt
    99841 ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga
    99901 gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta
    99961 tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct
   100021 gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac
   100081 tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca
   100141 gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc cgaatgagct
   100201 tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat tgtacctttc
   100261 gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgtctgga
   100321 ggtgttgtat cagtgcagtg ttcccaggcg ctgaacacca gccagattga tgtgcattgt
   100381 ttccgccata ttttgcagat tgttcgcgac aatgaagcag ctgaatatct ggagctgaac
   100441 gttggggata actggcggat tagcgaaggg aagccaaacc ctgagcagcc aatgcagatt
   100501 ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt
   100561 tcatcatcag aaccgctgct taacagtgtt tcatcgatgc tgggacgcgg tttgtacttt
   100621 aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc
   100681 cgcgaattgc acgactcgct ggctcaggtg ctttcttact tacgtatcca gttgacgtta
   100741 ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc
   100801 caggcgttga atgatgctta tcggcagtta cgcgagctgt tgactacttt ccgcctgacg
   100861 ctgcagcagg cggatctccc ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa
   100921 accagcgcta aactgaccct cgactgccgt ctgccaaccc tggcactgga tgcgcaaatg
   100981 caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac
   101041 gccagcgaaa tcgccatcag ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc
   101101 cgtgataacg ggattggtat cggtgaaccg aaagaacccg aaggtcatta tggactgaat
   101161 atcatgcgcg aacgcgcgga acggctgggc gggacgctga ctttttcaca accttccggc
   101221 ggcggcacgt tagtgagtat tagctttcgc tccgcagagg gtgaggggaa ggggaagggg
   101281 aacacgccgg ctgaggtgta aataattacc ctcacaccgg ccctctccct ttaaaggcga
   101341 gagggaaaaa cgtgcccata ttgacctgta agcgagtgtg tcagcaatct catgataatt
   101401 tacattaact cctttttttc tccacgattg gctcgtacct tgccgctaca gtgaagcaag
   101461 tcaagcctac aacaatacgc agaaacacga ggtcctcttt taatggcgaa tttctttatt
   101521 gatcgcccca tttttgcctg ggtgctggca atcctgttgt gtctgacagg taccctggcg
   101581 attttttcat tgcccgttga acaatacccc gatctcgcgc caccgaatgt gcgagtgacc
   101641 gctaactatc ccggcgcatc ggcccagacg ctggaaaaca ccgtgaccca ggttatcgag
   101701 caaaatatga ccggcctcga taatctgatg tatatgtcat ctcagagcag tggcaccggt
   101761 caggcatctg tcactttaag ttttaaagca ggcaccgatc cggacgaagc cgtgcagcaa
   101821 gtacaaaacc agctgcaatc agccatgcga aagttaccgc aggcggtgca aaatcagggc
   101881 gtgacggtgc gtaaaaccgg cgataccaac attctgacca ttgccttcgt ctctaccgat
   101941 ggctcgatgg ataaacagga tattgctgat tatgttgcca gtaatattca ggacccgtta
   102001 agccgcgtga atggcgtcgg ggatatcgat gcctatggtt cgcaatattc catgcgtatc
   102061 tggctggacc cggcgaaact caacagtttc cagatgacgg ctaaagatgt cactgatgcc
   102121 attgagtcac agaacgcgca gattgcggtt gggcaacttg gtggtacacc ttccgtcgat
   102181 aagcaggcgc tcaacgccgc cattaacgcc cagtcactgc tgcaaacacc agaacagttc
   102241 cgcgatatca ccttgcgggt gaatcaggac ggctcagagg taaggctggg cgatgtcgcc
   102301 accgtcgaaa tgggggcgga gaaatacgat tatcttagcc gcttcaatgg taagccagcc
   102361 tccgggttgg gggtaaaact ggcctccggc gcaaacgaaa tggcgacagc ggagctggtg
   102421 ctcaatcgtc tcgacgagct ggcgcagtat ttcccgcacg gactggaata caaggtggcg
   102481 tatgaaacca cctcgtttgt taaagcctcc attgaagacg tggtgaaaac gctgctggaa
   102541 gctatcgctc tggttttcct cgttatgtat ctgttcctgc aaaacttccg cgccacgctg
   102601 atacccacta tcgccgtgcc ggtggtgttg atgggaacct tctccgtact ttacgccttc
   102661 ggttacagcg tcaacacctt aaccatgttc gcgatggtgc tggcgatcgg tctgctggtg
   102721 gatgacgcca tcgtggtggt ggaaaacgtc gaacgtatta tgagtgagga aggactcact
   102781 cctcgcgaag ccacacgtaa atcgatgggg cagatccagg gggcactggt cgggattgcg
   102841 atggtgcttt cggcggtatt tgtaccaatg gccttcttcg gcggcaccac cggtgccatc
   102901 tatcgccagt tctctattac cattgttgcg gcgatggtgc tgtcagtact ggtagcgatg
   102961 atcctcactc cggctctgtg tgccacgcta cttaagccac tgaaaaaagc tgagcatcac
   103021 gggcaaaaag gtttttttgc ctggtttaac cagatgttta accgcaacgc cgaacgctac
   103081 gaaaaagggg tggcgaaaat tctccaccgt agcctgcgct ggattgtgat ttatgtcctg
   103141 ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga cgtcgttctt accgctggaa
   103201 gaccggggca tgtttactac ctcggtacaa ttgcccagcg gttcaaccca acaacagacc
   103261 ctgaaagtcg ttgagcaaat cgagaaatat tacttcatcc atgaaaaaga caacatcatg
   103321 tcggtgtttg ccaccgttgg ttctggccct gggggtaacg ggcaaaacgt ggcgagaatg
   103381 tttatccgcc tgaaagactg gagcgaacgc gacagtaaga ccggcacctc gtttgccatt
   103441 atcgagcgtg caacgaaggc gtttaaccaa attaaagaag ctcgcgttat cgccagcagc
   103501 ccaccagcaa ttagcggtct tggtagttct gcagattttg atatggagtt gcaggaccac
   103561 gctggagcgg gtcacgatgc gctgatggca gcacgtaatc agttgctggc gctggcggcg
   103621 gaaaacccgg agctaacccg tgtgcgccat aacggcctcg atgacagtcc gcagttgcag
   103681 attgatatcg accagcgtaa agctcaggcg ctgggcgttg atatcgacga tattaacgac
   103741 acactgcaaa ccgcctgggg ttcgagctat gtgaatgact ttatggatcg cggtcgcgtg
   103801 aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc tgccagatga catcaatctc
   103861 tggtatgtcc gaaataaaga tggcggcatg gtgcccttct ctgctttcgc gacctcacgc
   103921 tgggaaacag gctcgccgcg tctggaacgc tataacagtt attctgcggt tgagattgtt
   103981 ggggaagccg caccgggggt cagtaccggt acggcgatgg atattatgga atcgttagtg
   104041 aagcagctgc caaacggctt tggtctggag tggacggcga tgtcgtatca ggagcggctt
   104101 tccggcacgc aggctccggc gctgtacgcc atttccttgc tggtggtatt cctgtgtctg
   104161 gcggcattgt atgaaagctg gtcggtaccg ttctcggtaa tgctggtcgt gccgctgggg
   104221 gtaatcggcg cgctgctggc aacctggatg cgcgggctgg aaaacgacgt ttacttccag
   104281 gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg ccatcctgat cgtcgagttt
   104341 gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag cgacgctcca cgcctgccgt
   104401 cagcgtttac gcccgattct gatgacctcg ctggcattta tcttcggcgt attgccaatg
   104461 gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg tgggtactgg cgtaatgggc
   104521 gggatgattt cagctactat tctggctatt tacttcgtgc cgctgttctt tgtgctggtg
   104581 cgccgccgct tcccgctgaa gccgcgcccg gaataagcaa taaaaaaggc gacatgccaa
   104641 tgtgtcgcct ttttcaactt tccgattaag aacctgctca gcgggttctt gctgtttata
   104701 ctttgtctca ggaattactt acgaagcata acttcgataa agtctttcca gttccccagt
   104761 tcacgttcaa tcataacaac ctctcttata attatgggta ttctacggaa aaaatatacc
   104821 gtggtgaagc taatttactc gattgctgcg atgactacct ccgggggaca aaccttatgt
   104881 aaatactatg gtcctgcggt gataatttgt atgtgataca cagcaacatt tcgagatatt
   104941 catacggcat ctaatactta tttaattctg gttaaaatac agacagataa caagatgaat
   105001 attcttaatg tttacgttaa aaatgtttaa tattatttaa tagttgttaa tttgaatact
   105061 tcgataatgt tatattttct gataatcatt tgcaggcaaa atgttttcac ccttaaatga
   105121 gtatttattc tcataaatcg aaaaaggatt cattatggtt acactttacg gcatcaaaaa
   105181 ttgtgacacc attaaaaagg ctcgccgttg gctggaagcc aataacatcg actatcgttt
   105241 tcatgattac cgcgtcgatg ggctggacag cgaattattg aacggtttta tcaacgaatt
   105301 aggctgggaa gcgttactca acacccgtgg tacaacctgg cgtaaactgg acgaaactac
   105361 ccgcaataaa atcaccgatg cggcctctgc ggcggcatta atgactgaaa tgcctgcaat
   105421 tatcaaacgt ccattgctct acgcgcctgg taagcctatg ctgctgggtt tcagtgattc
   105481 cagttatcag caatttttcc atgaggtgta gtctatgtcg tgcccggtta ttgagctgac
   105541 acaacagctt attcgccgcc cttccctgag tcctgatgac gcaggatgtc aggctttgtt
   105601 gattgaacgt ttgcaggcga tcggttttac cgttgaacgc atggattttg ccgatacgca
   105661 gaatttttgg gcatggcgtg ggcaaggtga aacgttagcc tttgccgggc ataccgacgt
   105721 ggtgccgcct ggcgacgccg atcgttggat caatccgccg tttgaaccca ccattcgtga
   105781 cggcatgtta ttcgggcgcg gtgcggcaga tatgaaaggc tcgctggcgg cgatggtggt
   105841 agcagcagaa cgttttgtcg cacaacatcc caaccatacg gggcgactgg catttctgat
   105901 cacttctgat gaagaagcca gtgcccacaa cggtacggta aaagtcgtcg aggcgttaat
   105961 ggcacgtaat gagcgtctcg attactgcct ggtcggcgaa ccgtcgagta tcgaagtggt
   106021 aggtgatgtg gtgaaaaatg gtcgtcgcgg atcgttaacc tgcaacctca ccattcatgg
   106081 cgttcagggg catgttgcct acccacatct ggctgacaat ccggtacatc gcgcagcacc
   106141 tttccttaat gaattagtgg ctattgagtg ggatcagggc aatgaattct tcccggcgac
   106201 cagtatgcag attgccaata ttcaggcggg aacgggcagt aacaatgtta ttccgggtga
   106261 actgtttgtg cagtttaact tccgcttcag caccgaactg actgatgaga tgatcaaagc
   106321 gcaggtactt gccctgcttg aaaaacatca actgcgctat acggtggatt ggtggctttc
   106381 cgggcagcca tttttgaccg cgcgcggtaa actggtggat gcggtcgtta acgcggttga
   106441 gcactataat gaaattaaac cgcagctact gaccacaggc ggaacgtccg acgggcgctt
   106501 tattgctcgc atgggggcgc aggtggtgga actcgggccg gtcaatgcca ctattcataa
   106561 aattaatgaa tgtgtgaacg ctgccgacct gcagctactt gcccgtatgt atcaacgtat
   106621 catagaacag ctcgtcgcgt gatgagtggt tctgcaagag gaaataagca tggactggct
   106681 ggctaaatat tggtggattc tggtgattgt ctttttggtc ggcgtcctgc tgaacgtgat
   106741 taaagatctc aagcgcgtcg accataagaa atttctcgcc aacaagccgg agcttccccc
   106801 gcatcgtgat ttcaacgata agtgggacga tgacgacgac tggccgaaaa aggatcaacc
   106861 gaagaagtaa gctgagtgct caaccgtagg ccggataagg cgttaacgcc gcatccggca
   106921 atggtgaacg atgcctgatg cgacgctcac gcgtcttatc aggccaggta atctgtcttt
   106981 tacatcattt caatcacatc atcatcgcca ggcttaccgc cacttagcgc ttggtcgaaa
   107041 taatgtttcg gaatggtata acgcaagtga tccagggcaa attgcatact gcgattgtcg
   107101 atcgcatggc ctaaatcctc cacgatatcc agcgtcacgt caccaccggc actgattaac
   107161 gcttcctgcg ccgcaactgc atgcgccaga tcgataaccg gatcttcacc accatgaatc
   107221 aagtgaattg tcgtggctgt agacgcggtc tccggcaggc tcgcatagcg tccgttaaag
   107281 gcaatgacgc gcgaagcaag gcccggttcg gctttaatgc tctccagcgc cataatcgcg
   107341 ccttgtgaga aaccaattag cgccgtggcg ttagccccta ctccgctctg tttctgccag
   107401 tagcgtaccg tttcaataaa cgtcggcatg atggcatcca cacgcgcctg gcgattatct
   107461 tccgtaatac cctgcactga aaaccactga cgccccgccg gattaccgct cggctccgcg
   107521 ccaccgacac tcaccaccag cgcatcagga aacagcggtg caaaccagct gcctatttcc
   107581 cccattgcca ccgggttatc cccgacaccg tggaaaagca gtaacaactg ttgtgcaggt
   107641 ttatccgggc tttgaacaac aaaatggtca tgtttcatgg cgaactcctt aactgatgtc
   107701 attaatttta cgccgctgac tcacaatgac catgggagaa aattgaagga gtcagtgaaa
   107761 aaattgccat tgcgttatgc gatcgcgcag acgctcagtg cgaacctcat ttagtgcgaa
   107821 cagcgcctgc gcggcctctt cccgctggcg aaccagtaac atcttgcggc ctgaaagttt
   107881 aagtgtggta cataactgcg cgtcactggc gtttttctgt aaacacccac gcagcgccgg
   107941 taatgccagt tcactggttt gtagcagacg caataagcaa cctaacgatg ttaatagcgg
   108001 acgatgagcg aaagcaaaac cggccagttc aagccagtcg tcgtcagaaa ggatggcatc
   108061 gtttagtgga tcaacaggaa gcatttcgcc attccactgc gcgagagctt gcgcatcgcg
   108121 acgtaaacgg taatgctcac gttcagccag ctgtttaccc gcatcactca tcggtaacag
   108181 cgccatcgcc gtatagcaac cgctgctggc ttcccgatga ttacccatcc gcaccagcac
   108241 aaaaccgcag cgttgccaga aacgccataa ctccccagtg taaccaaaac tcaccgaaag
   108301 atagtcgagg tcatgcgtat attgcaaagc tccagcaata agttgccgcc ctgtgccttc
   108361 ccgctgacga gccggatgaa ctgctatccg gctaacccgc cttccgcgca atgtcgccgc
   108421 cagtggattg ctgccgtgcg ccgccagcga ctgggccacc agattacccc gcgggcgacg
   108481 aaaacctgcc cataccgcct gactgagttc ttgagataat ccgccctcat ccaccagcca
   108541 caacgccccg gcaatctcgt tttcgccagc cgcctgtaaa aaatgttgcc ctagtgcatc
   108601 catcatccgg cgtaaatcca gcggcgaggt ccggtagtgc gcaccagaca ataactgata
   108661 aacctttaac ggcgtttctg gctcgcttcg ccataacgtt tgttcaaatg cggaaatgac
   108721 gatattgcct tgtggtgtat gggtgaagtt ttcatcgtta aaaaccagtg cctcactaac
   108781 cattttttcc agcgggcatc cctgtgccca gcggatcggc tgttgcagtt caaaacggtg
   108841 taaatgcgga aagcgagcgc aaaatttcag caaaaaacca cgtccggtac cttcgtagcc
   108901 ctgcaccgta gtggttaaca acgttcgagg aaaacgcgat accagttgat gcaacaacgg
   108961 cgcaggtatg gctgcggctt cgtcgaccac cagccagtcg gcttgctcat cgctggctaa
   109021 caaggcatcc ggtgcaataa agcgaaactt ctcgcccgca aattgtgcca gtacatccgt
   109081 tgccgctttt gcgggcgcgg tgacaatcgc actgcccgca atacgagaaa tgagttgccc
   109141 tgccaacgcc gacttaccgc gcccacgcgc agccgttacc gctgccacgc ccggcggcat
   109201 ggtcagtagc tgctgtaaga gttgctgttg ttctggttgt ggtgcgccgg tcgcggggtg
   109261 ccagtcagta cggggagtaa aatgcgccaa cgagaacggc tggttttgcc gccagaggat
   109321 agcgtcgtta tccgccgtaa gtacgcgttt gagatgctgg acaaaatgcg gcgtcgcaat
   109381 agggtcaggg caatcactcc agcgcagcga gtcggcatca ggttggtttt cccactcttc
   109441 ccatacaggg agtaacaaaa ccagccagct tcccgctttc aacgttccgc taagtgctgc
   109501 aaaggcagcg gcatcaaagc cgtggcgggc gtcgaatacc gcatgccgga actcgcgccc
   109561 aagtaaagtt tgtagcgccg agggtgaaca gtggttttca gcatctagct gcggcgaaat
   109621 ccacagccag tcgccaggta aggcatcacg caacttaaga gcatgatcaa aacaccaacc
   109681 ctcttctccg ctcaacacca gcaagcggca gatcccttca cgtttcattt gcgctgttaa
   109741 tgtgtaaagc gcagtcagtt cagccatccc tgccccggaa attaaatgct tttaccaaaa
   109801 gtattgcatt gtgccggatc gccgctgtcg aaaccacgtt taaaccagct gcagcgttgc
   109861 tgagaagtgc catgtgtgaa actgtctggt actactcgcc cctgactttg ctgttgtaaa
   109921 cggtcatcgc cgatggcctg cgccgcattc agcgcctctt ccagatcgcc agtttccaga
   109981 acgccttgct gctgcatact atgcccccag acaccggcaa aacagtcggc ctggagttcc
   110041 atacgcacag ataagcggtt tacttccgcc tgcgtcgcgt tttgttgcag ttgacgaact
   110101 ttcggctcga tgcctaacag tttctgcaca tgatgaccga cttcatgggc gataacgtac
   110161 ccctgggcaa aatcgccatc cgcgccaagt ttgtctttca tgtcatcata gaaggagaga
   110221 tcgatataaa ctgtgccatc cgccgggcaa tagaacggcc ccataatgga ctggcccgcc
   110281 ccgcagccgg tgcgcgtcat tccacggtac atgaccagct tcggttgctg ataggtctta
   110341 cccatctttt cgaactgttg tccccaggtg tcttccgtgg ttgccagaat caccgaggtg
   110401 aattttgcgg cttcgtcttc attagggcta attgaccgcg ttgattgttg ttgagaaacc
   110461 ggctgcccgg tcatcaaccc ggttaaatca acgccatagt agcctgcaac cagcacgact
   110521 atcagtaaaa tcagcccgcc tttaccgctc ggcagacgga aaccgggacc gcccattgac
   110581 ggaccgccag agctgttgcg cctgtcttca acattgtcac tttcacgtcg cccttgccaa
   110641 cgcatagata cctcaacaat atattcatta tagaaatgat cgtaggtggt tcagaggaag
   110701 attaccacag gaaagcggga tgaaatggcg gaggaatgag cgttcaatgc atatattccg
   110761 agagcggttg tcttgccgtg ccagctgcac ggcaagatga tgaacagaaa aatcagtcca
   110821 gctgtacacc caggcggcgg gcgacggctt cataggcttc gatcaggcca ccgaggctct
   110881 ggcggaaacg gtctttgtcc attttctcca gtgtttcttt gtcccacagg cggctgccgt
   110941 ccggggagaa ctcatcgccc agtaccactt cacctttgta cagaccaaac tccagcttga
   111001 agtcgaccag aatcagacca gcatcatcga acagtttttt cagcacgtcg ttcgctttgt
   111061 aggtcagctc tttcatacgt gccaggttct ctttgctcac ccagccaaag gtttcgcagt
   111121 aagattcgtt gaccatcgga tcgtgcatgg catcgttttt caggaacaga tcgaacagcg
   111181 gcgggttcag ctcaatacct tcttcgattc caagacgttt caccagagag ccagcagcac
   111241 ggttacgcac gacacactca accggcacca tatccagctt tttcaccaga cattcggtat
   111301 cggagagcag acgctccatt tgagtcggga tacccgcttc agccagtttg ctcataatga
   111361 agtagttgaa cttgttgttc accatacctt tgcgatcaaa ctgctcaatg cgcgcgccat
   111421 cccctgctga cgtatcattg cggaattcga gcaccaacag gtccgggttt tccgtgctgt
   111481 atacggtttt cgctttacca cgatacaact cagcttgctt ttgcatcttt atcactcctg
   111541 ggtgtgaatt aacgttttaa aatcttttgc tgtctggtgt gccggatgtt ttttcggatg
   111601 cggcgtgaac gccttatccg aactacgtcc gacaaacaaa tttcgtgcga attaccgcta
   111661 aaatcgcgtt ttcctgccga cgcacacgtt tgcgtatcat atcagaaaaa agggccggat
   111721 gattccagcc ctgtattttt acttgctaaa cgcagcctgg aagacagcta ccagcgcgtc
   111781 gttctgactc tgagtcagag tatgaccttt cggatcgatg aactgtaggc tgctgcggtt
   111841 atctaaatcg ccaacctgca gtttatagtc accggatgcc aggcctggat cgctcgcgcc
   111901 cagttcctgc cagtcgctgt cagacagcgg cttataagtt acggccatgt tgccctgcga
   111961 acgggtgctg tcggtcactt tcatgcccac tttttccagc gacgctggca gacgttgcca
   112021 aaccacattg aacggcccgc gtacgaccag cattggtaaa ccggtgtcat cagctgcact
   112081 ttgtacgtcc atagtggtgg aggcacgatt ttgcgcagcg ttcgcggcgt cagtggcaga
   112141 tttatccaga ccggcggaaa taacgttcat catctccgtg ctgtaacgct gcatggaagc
   112201 cgcgtctgca accggtttgc ccgcctgttc caggttcagc agtttaaccg taaccgcctg
   112261 ctgataaccc tgcggcttaa cagagatttg ataacgacca cgatactgct cgtcttcgtc
   112321 cagacggttc cattgtaccc aatcggtggt cagtgtctga ccagcatcat cacgttgggt
   112381 gatggtgtag tttttcgcct gcagcacgct aaccacctgc ggccacagag tattgccacg
   112441 accattttcc accagcaatg aagcggtatc gcccgtgaac tgggtacgcg cgccagaaac
   112501 cagtgccagc ggctgggctg gtggacgaat gtccagcgcc ttaccgacag caccactacc
   112561 gttggtcacc gggattgcat aatcaccgga ggtcaccggc aaaatcattc cagccggggc
   112621 atgaagctcc gcaagcggtg ccgcttccag gtaggcttca tcaccactga cctgacgctt
   112681 atagcgtgag tcagaactac aggcagcgag taataaaaca agcgaaacac ccgcaacctt
   112741 tgccaggcgc gacttttgaa cagagtaagc catcaaatct ccctaaactt tacagcaaac
   112801 cggcatgctt aagcgccgct ctgaccgtct cacgaccact gtcggtgatt ggtgtcattg
   112861 gcaggcgcag cgtatcggtc gccacaagac ccagttcctt acatgcccat ttcaccggga
   112921 ttggattggg ttcgacaaat agtttgttgt gtaatggcat cagacgctga ttaataacgc
   112981 gtgcctcggc aaaatgccct tctgctgcca gtttgcacat ctgggccata tcacgcgctg
   113041 cgacgttagc cgtaacggaa ataaccccat gaccgcccaa ttgcatgaag tccagcgcgc
   113101 tcgcatcatc gccgctcagc agaacaaaat catctgaaac cagctctttg atctggttta
   113161 cacgcgttaa gttccctgtt gcctctttga ttccgataat attttttact ttcgccagac
   113221 ggcccaccgt ttccgggagc agatcgcagc cagtacggga cggcacatta tacagaattt
   113281 gcggcaggtc agtatgctca gcgatggctt tgaaatgctg atacaaacct tcttgcgacg
   113341 gacgattgta gtaaggggtt accgtcaggc agccgacgat accactgtca ttgaagcgct
   113401 gcgtcaggct aatggcttcc gcagtagcgt tagcgccggt cccggcaatt accggaatgc
   113461 gcccatcagc cagatccagc gtcatcatca ccacatcagc atgttcgtca tgatttaagg
   113521 tagcggactc gccagtggtg ccaacagaaa cgatcgccga agtaccgctg gcgacatgat
   113581 aatcaatcag ttttttcaag ctagcccgac agacattacc tttttcatcc atcggagtaa
   113641 caatcgcgac aatacttccc gtgaacatgg gccatcctct gtgcaaacaa gtgcctcaat
   113701 ggtacgtttg gtatggcatt aaaagcaagc agacagaacc gttctgattg ttgtatgcat
   113761 gattttttta tgctttcctt aagaacaact caccccttaa aggaataacc agtttgacac
   113821 tgtcatcgca acattatctg gtgatcactg cgttgggtgc cgatcgccct ggaattgtga
   113881 acaccatcac ccgtcatgtc agtagttgcg gctgtaatat tgaagacagt cgcctggcga
   113941 tgctgggaga agagttcacg tttatcatgc tgctttccgg ttcatggaat gccatcactc
   114001 tgattgaatc aacgttaccg ttgaaaggtg ccgaactgga tcttttaatc gtgatgaagc
   114061 gcacgacggc gcgtccgcgt ccgccaatgc cagcatctgt ctgggttcag gtcgatgttg
   114121 cagactcccc gcatttaatt gaacgcttca cagcactttt cgacgcgcat catatgaaca
   114181 ttgcggagct ggtgtcgcgc acacaacctg ctgaaaatga acgggctgcg cagttgcata
   114241 ttcagataac cgcccacagc cccgcatctg cggacgcagc aaatattgag caagcgttca
   114301 aagccctatg tacagaactc aatgcacaag gcagtattaa cgtcgtcaat tattcccaac
   114361 atgatgaaca ggatggagtt aagtaatgaa tccactgaaa gccggtgata tcgcaccgaa
   114421 atttagcttg ccggatcaag acggagaaca agttaatttg accgacttcc agggacagcg
   114481 tgttctggtt tatttctacc cgaaagccat gacccccggc tgtaccgtac aggcctgcgg
   114541 cttacgcgat aacatggatg agttgaaaaa agcgggcgtt gatgtgctgg gtatcagcac
   114601 cgataaaccc gaaaaactct cccgttttgc ggaaaaagag ctgcttaact ttacgctcct
   114661 gtctgatgag gaccaccagg tgtgcgaaca attcggcgtc tggggtgaaa agtccttcat
   114721 gggcaaaacc tacgatggta ttcatcgcat cagcttcctg attgacgctg atggcaaaat
   114781 cgaacatgtc tttgacgatt tcaaaaccag caatcaccac gacgttgtgc tgaactggct
   114841 gaaagaacac gcctgattac tttgctccat tccgtgctgg ctgcgcttgc ggccagcata
   114901 cctcacttct cgtgatcaag atcacattct cgctttcccc tgcgacacgg gtgtcgaatc
   114961 cattttttgc tgaacgttaa tgaccatcat ttttgtaccg ttcagaatcc agttaataca
   115021 taacttattg aatagattga gttaatcaga atggcaccct ttatgcaata tgaaatgcaa
   115081 tgtttcatat cattttcaag gagccgacat gaaccgcttt gtggtggccg aaccactgtg
   115141 gtgtacagga tgtaatacct gtctcgctgc ctgttcggac gtgcataaaa cgcaaggttt
   115201 acagcaacac ccgcgcctgg ccctggcgaa gacgtcaaca atcactgccc ctgtcgtgtg
   115261 tcatcactgt gaggaagccc cttgcctgca ggtctgcccg gtcaatgcca tctctcagag
   115321 ggatgatgcg atccaactca acgaaagcct ctgtattggc tgcaagcttt gcgccgtggt
   115381 ctgcccattt ggcgcaatca gcgcttcagg aagccgtccg gtgaatgccc atgcgcaata
   115441 tgtttttcag gctgaaggct cactcaaaga cggcgaagaa aacgtgccgc cccaacatgc
   115501 gttgctgcgc tgggaaccgg gtgtccagac cgtcgcggtg aaatgcgacc tgtgtgattt
   115561 cttgccagaa ggtccggcct gcgttcgcgc ttgcccgaat caggcgttac ggctgatcac
   115621 cgacgatagc ctgcaacgtc agatgaaaga aaaacagcgc cttgccgcaa gctggtttgc
   115681 caatggcggg gaggatcccc tttccctcac tcaggagcaa cactaatgga tgccctgcaa
   115741 ttattaacct ggtcgctgat cctctatctg tttgccagtc tggcttcgct gtttttactc
   115801 ggtctggaca gactggctat taagctttcc ggcatcacat cgctggtggg cggcgtgatt
   115861 ggcatcatca gcggaattac gcaattacat gctggtgtaa ctttagtcgc ccattttgcc
   115921 accccttttg actttgccga tttaaccctg cgaatggata gcctctcggc atttatggtg
   115981 ctggttatct ccttgctggt ggtggtttgt tcgctctatt cattgactta tatgcgcgaa
   116041 tacgagggca aaggcgcggc ggcgatgggc ttctttatga atcttttcat cgcatcgatg
   116101 gttgccctgc tggtgatgga caacgctttt tggttcatcg tgctgtttga aatgatgtcg
   116161 ctgtcttcct ggtttctggt cattgccagg caggataaaa cgtcgatcaa cgctggcatg
   116221 ctctactttt ttatcgccca cgccggatcg gtgctgatta tgatcgcctt cttgctgatg
   116281 gggcgcgaaa gcggcagcct cgattttgcc agtttcgcac gctttcactt tctccggggc
   116341 tggcgtcggc ggtgtttctg ctggcctttt tcggttttgg cgcgaaagcc gggatgatgc
   116401 cgttgcacag ctggttgccg cgcgctcacc ctgccgcacc atcgcacgct tcggcgttga
   116461 tgtctggcgt aatggtcaaa atcggtattt tcggcatcct gaaagtagcg atggatctgc
   116521 tggcgcaaac gggtttgcct ctgtggtggg gcattctggt gatggcgatc ggcgcaatct
   116581 ccgcgctcct gggcgtgcta tatgcgctgg cggaacagga tatcaaacgg ctgctggcct
   116641 ggagtaccgt cgaaaacgtc ggcattattt tgctggcagt cggtgtggcg atggacggtc
   116701 tgtcactgca cgacccgctg ctcaccgtgg ttggactgct cggcgcactg tttcatctgc
   116761 tcaaccatgc gctgttcaaa gggctgctat ttctcggcgc ggacgcgatt atttcgcgtt
   116821 tgcataccca cgacatggaa aaaatggggg cactggcgaa acggatgccg tggacagccg
   116881 cagcatgcct gattggttgc ctcgcgatat cagccattcc tccgctgaat ggttttatca
   116941 gcgaatggta cacctggcag tcgctgttct cactaagtcg tgtggaagcc gtagcgctac
   117001 aacttgcggg tcctattgct atggtaatgc tggcagtcac tggtgggctg gcagtaatgt
   117061 gcttcgtcaa aatgtacggt attactttct gtggtgcgcc gcgcagtaca cacgctgaag
   117121 aggcacagga agtgccaaat acgatgatcg tcgccatgct actgctcgcg gcactctgcg
   117181 tattaattgc gcttagtgcc agttggctgg caccgaagat aatgcatatt gcccatgcgt
   117241 ttaccaatac ccctcccgtc actgtcgcca gcggaatagc acttgtaccc ggcacgtttc
   117301 atacacgggt cactccttca ttactgttgc tgttactact ggcgatgcct ttgctgccag
   117361 gcctttactg gctgtggtgt cgttcgcgcc gcgcagcgtt tcgtcgcaca ggagatgcct
   117421 gggcatgcgg ctacggctgg gaaaacgcga tggctccgtc aggcaatggc gtgatgcagc
   117481 cgctgcgtgt ggtcttctct gcgttatttc gtctacgaca acagctcgac cctacgctga
   117541 ggctaaataa aggtcttgcg cacgtcaccg ccagggctca gagcacagaa cccttctggg
   117601 atgagcgggt gatccgcccc atcgtgagcg ccacccaacg gctggtcaaa gaaatacagc
   117661 atctgcaaag cggcgacttt cgtctctatt gcctgtatgt ggtcgccgca ctggttgtgc
   117721 tgctaatcgc tattgccgtc taaggaaatc accatgagac aaactctttg cgacggatat
   117781 ctggtcattt ttgcgttagc acaggccgtg attctgctga tgctaacccc actttttacg
   117841 ggtatttccc ggcagatacg cgcgcgtatg cactcccgcc gcgggccggg gatctggcag
   117901 gattatcgcg atatccacaa actgtttaaa cgccaggaag ttgcgccgac atcttcaggt
   117961 ctgatgttcc gcctgatgcc gtgggtatta atcagcagca tgctggtgct ggcgatggcc
   118021 ttaccactat ttattaccgt ttcccctttt gcgggcggcg gcgatctgat cacccttatc
   118081 tatcttcttg ccctgtttcg ttttttcttt gctctttccg ggctggatac cggaagtccg
   118141 tttgcgggag tcggtgccag tcgcgagttg acgctcggca ttctggtcga accaatgctt
   118201 attctctcac tgctggtatt ggcgctgata gcaggttcca cgcatatcga gatgatcagc
   118261 aatacgctgg cgatgggctg gaactcgccg ctaaccaccg tactggcgtt actggcctgt
   118321 ggttttgcct gcttcattga gatgggaaaa attccctttg atgttgctga agcagaacag
   118381 gaattacagg aaggcccgct gaccgaatat tccggtgccg ggctggcgct agcgaaatgg
   118441 gggcaggggc tgaaacaggt cgtgatggca gcactgtttg tggccctgtt tctgcccttt
   118501 gggcgcgcgc aagaactttc tctcgcctgc ctgctgactt cacttgtcgt tacgctgctc
   118561 aaggttttgc tgatttttgt actggcctca atcgcagaaa acacgctggc acgcgggcgt
   118621 tttttactca ttcaccatgt gacctggctt ggcttggctt cagccttgct gcgcttgcat
   118681 gggtcttctg gttaaccggt ctgtaaggag cactgacgga atatggaaaa tcttgctctg
   118741 acgacgttaa tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc acaacgtcgg
   118801 gccgccgaat ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt gtcgctgatc
   118861 tccgcgtttt atcaggccga taaagttgcc gtcacgttga cgttggtcaa cgtgggggat
   118921 gtggcattgt ttggcctggt cattgatcgc gtgagtacgc tgattctgtt tgtggtggtg
   118981 tttctcggtt tgctggtcac gatctactcc acgggttatc tgacggataa aaatcgcgaa
   119041 cacccgcata acggcacgaa tcgttattac gcatttttgc tggtgtttat cggcgcgatg
   119101 gcgggactgg tactctcctc gacgctgctc ggtcagttgt tgttttttga aattacgggc
   119161 ggctgctcct gggcgttgat cagttattac cagagcgata aagcgcagcg ttcagcacta
   119221 aaagcgttgc ttatcaccca tatcggttcg ttggggttgt atcttgccgc cgccacgctg
   119281 tttttgcaga ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt
   119341 tatctggttt atggcggcat cctgtttgcc gcgtggggga aatcggccca gctaccgatg
   119401 caagcgtggc taccggacgc aatggaagcg ccaacaccga tcagcgacta tctccacgcc
   119461 gcatcgatgg tgaaagtggg cgtttacatt tttgcccgcg ccattatcga cggcggcaat
   119521 atcccgcatg tgattggcgg cgttggcatg gtcatggcac tggtcaccat tctttatggc
   119581 tttctgatgt atttgccaca gcaggatatg aagcggttgc tggcctggtc gaccatcact
   119641 caacttggct ggatgttctt cggcttgtcg ctctccatct tcggctcgcg gctggcgctg
   119701 gagggtagca tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt tttccttgta
   119761 gcaggtgcgc tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg tggcgtattg
   119821 cacaccctgc cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat taccggtgtg
   119881 ccgccgttca acggcttctt cagtaaattc ccgctgtttg ccgccggttt tgcgttgtca
   119941 gtggagtact ggatcctgct gcccgccatg attctgctga tgattgaatc ggtcgccagt
   120001 ttcgcctggt ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag cgaggccgtc
   120061 gccgatgccg caccgctgcc agggtcaatg cgcctggtgt tgattgtact gattgtgatg
   120121 tcgctgattt ccagcgtaat cgccgcgacc tggttgcagt aaggagatga tgaatgaccg
   120181 gttctatgat cgtaaataat ctggcgggac tgatgatgct gacatcgctg tttgtgatta
   120241 gcgtcaaaag ctatcgcctg tcatgcggat tttacgcctg ccagtcgctg gtgctggtgt
   120301 ctattttcgc cactctctcg tgcctgttcg ccgcagagca actgctgatc tggtccgcca
   120361 gcgcctttat caccaaagtg ctgctggtac cgttaatcat gacttacgct gcacgaaata
   120421 ttccccagaa catcccggaa aaagcgttat tcggtccggc aatgatggca ctgctcgcgg
   120481 cgttaattgt cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg atggctaccg
   120541 ggctgaaacc ggcgctggcg gtagcgttag gtcattttct gcttggcctg ctgtgcattg
   120601 tcagccagcg caatatcctg cggcaaattt ttggttactg cctgatggaa aacggctccc
   120661 atctggtgct ggcgcttctt gcctggcgag caccggaact ggtggaaata ggtatcgcta
   120721 ccgacgccat cttcgccgtc attgtgatgg tgttactggc aagaaaaata tggcgtaccc
   120781 acggcacgct ggacgtgaac aacttgaccg cgctgaaggg ataatgagat gagttattct
   120841 gtgatgttcg ctttactcct gctcacgccg ctgctttttt cgctgctctg ttttgcctgc
   120901 cggaaacggg ggctctctgc gacttgtacg gtgactgtat tacatagctt agggatcaca
   120961 ctgctgctga ttctggcact ctgggtggtc caaactgccg ctgatgcagg agaaatattc
   121021 gctgcgggac tgtggcttca tattgatggt ctgggcggtt tgttcctcgc cattcttggt
   121081 gtgattggct ttctcaccgg tgtttactcg attggctaca tgcgtcatga agtggcacac
   121141 ggcgagcttt cacccgttac actgtgcgat tactacggtt tcttccatct gttttttttc
   121201 accatgctgc tggttgttac cagcaataac ctgattgtga tgtgggcggc gatcgaagcc
   121261 accaccttaa gctcggcgtt tctggtaggc atttacggtc agcgttcatc gctggaatca
   121321 gcatggaagt acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt cggtaccgtg
   121381 ctggtatacg ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat attctggagc
   121441 gaggttctta agcaatcgtc cttgcttgac ccaacattaa tgctgttggc ctttgtgttt
   121501 gtgctaattg gctttggtac caaaaccggg ctatttccca tgcacgcctg gctgccggat
   121561 gctcacagtg aagcgccgag tccggtcagc gccctgctct ccgccgtatt gctgaactgc
   121621 gcgctgttgg tgctgattcg ctattacatc attatttgcc aggccatcgg cagcgatttc
   121681 cccaaccggt tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc atttttcatt
   121741 ctggtacagc gggacattaa gcgtctgctg gcgtactcca gcgtggagaa catggggctg
   121801 gtcgcggtgg cgctaggcat tggcgggccg ctgggaattt ttgccgcgct gctgcacacc
   121861 ttaaaccaca gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt actgctcaag
   121921 tacggcacgc gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc atttaccgcc
   121981 gtgctgtttg gcggcggtgc gctggcgctg gcagggatgc cgcccttcaa catttttctt
   122041 agcgaattta tgaccgttac cgcaggactg gcacgtaatc acctgctgat tatcgtcctg
   122101 ctgttattgc tgttaacgct ggtgctcgcg ggcctggtac ggatggctgc gcgggtgtta
   122161 atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg gctggttgac cacctcgcca
   122221 atggtgattc tgctagtcat gatgctggcg atgggaacgc atattccaca acctgtcatc
   122281 aggatcctgg cgggcgcttc cactatagtc ctctcaggga cgcacgacct gcctgcacaa
   122341 cgtagcacct ggcatgattt tttgccttca ggcaccgcat ctgtttcgga gaaacacagt
   122401 gaacgttaat tcatcgtcaa atcgtggcga agcgattctc gccgccctga aaacgcagtt
   122461 ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa caggtcacca ttacggtgaa
   122521 aatcaatctg ctgcctgacg ttgtacatta tctttattat caacatgatg gctggcttcc
   122581 agtcctgttt ggcaacgacg agcggacact taacggtcat tacgcggttt attatgccct
   122641 ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag gcactggtcg atgccgacag
   122701 tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg gtctggggcg agcgagaaat
   122761 tcgcgatatg tacgggctga ttccggttgg cctgccggat cagcgtcgcc tggtgttgcc
   122821 cgatgactgg ccggaagata tgcatccgct gcgcaaagat gcgatggatt atcgactgcg
   122881 ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc aatgagggca acagcgatgc
   122941 gcgggtgatc cctgtcggcc cgctgcatat cacctcctat gaaccgggtc acttccgctt
   123001 gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc ctgttttatg tccatcgcgg
   123061 catggagaaa ctggcagaaa cgcggatggg ctacaacgaa gtgaccttct tatcggaccg
   123121 cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat accaactcgg ttgaaaatgc
   123181 actggggatt gaggtgccgc aacgagcaca tactattcgc tcgattctgc tggaagtcga
   123241 gcggctacac agtcatttgc tcaaccttgg cctctcctgc cattttgttg gttttgatac
   123301 cggctttatg caatttttcc gcgtgcggga aaagtcgatg acgatggcgg aattgctgac
   123361 cgggtcgcgt aaaacctacg gtctgaatct gattggtggt gttcgccgcg atattctcaa
   123421 agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg cgctccgacg tgtcggagct
   123481 ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc actcagggca ttggcattct
   123541 cgaccgacaa atcgcccgtg attatagccc tgtagggccg ctgatccgcg cagtggtttt
   123601 gcccgtgatt tgcgctttga tcacccctac gccgactacg gcaatattcc aaaaacactg
   123661 tttaccttta ccggcggcga tgttttctcc cgcgtgatgg tccgtgtcaa agagacgttt
   123721 gattcgctgg caatgctgga atttgccctc gacaacatgc cggatacccc actgctgacc
   123781 gaaggcttta gctataaacc tcacgcattc gcgctgggct ttgttgaagc gccacgcggt
   123841 gaagacgtgc actggagcat gctcggtgat aaccaaaaat tgttccgctg gcgctgtcgt
   123901 gccgccacct acgccaactg gccggtgttg cgttacatgc tgcgcggcaa taccgtttct
   123961 gacgcaccgc tgattatcgg tagccttgat ccctgctact cctgtaccga ccgtgtgacg
   124021 ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt ataaagagat cgaacgctac
   124081 ggcattgatc gtaaccgttc gccgctgaag taaggacaga agatgctgaa gttactgaaa
   124141 actattatgc gcgccggaac cccgacggtg aaatatccct tcgcgccact ggaggtcagc
   124201 ccaggctttc gcgggaaacc ggacctgatg cccagccaat gtattgcctg cggtgcctgc
   124261 gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca aaattcgcgc
   124321 acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga agtgtgcccg
   124381 accagagcca tccagcttac caataacttt gaactgaccg tcacccataa agccgatctc
   124441 tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc gtttgccccg
   124501 caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc tccacaaaac
   124561 cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg cgcgacgctg
   124621 atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt ccagtgctta
   124681 cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg aagcggcatt
   124741 tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc ggctgcaacg
   124801 cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa cgttttggca
   124861 ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc gcagtcaccc
   124921 gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat aaaatctgtg
   124981 tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac agcgtctggg
   125041 gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg ccaacaccgg
   125101 ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag attcacgcgg
   125161 tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag atcccgccat
   125221 cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt caggggcgag
   125281 aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat cgggttaaca
   125341 cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa ctctttcgcg
   125401 tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac gctgcgaaaa
   125461 aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat gtattactcg
   125521 ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt ccgctgccca
   125581 ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg acgtaagatg
   125641 ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc cctgttgacc
   125701 cgggcattcg cgccactggc agatgacgcg acgtctgtgt ggcaagcgcg tagcattcaa
   125761 ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat ggccagaaaa
   125821 attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca gacgaagcga
   125881 tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc gcggagcggt
   125941 tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg ccgccgccgt
   126001 tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat taccgctacc
   126061 atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg gtcgattatt
   126121 cgcgtcacgc cgtccttctc ggcagtagtt gaatggcgga gctacgcttt attcgtaccc
   126181 acggcagccg ttttactccc caggattgca cactgtttaa ctggctggca cgcattatca
   126241 ccccggttct gcaatcatgg ctcaatgatg aagaacagca ggtggcgctg catttgctgg
   126301 agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg ctgtcacatc
   126361 ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt ttcggtctgg
   126421 cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc ctgtggtgca
   126481 gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa tatgcctggc gacagtgtat
   126541 tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca gatgatctgt
   126601 ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc gaatctgcgc
   126661 tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg gcgcatacct
   126721 cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc gatcgcatcg
   126781 ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac cgatttgcag gaaagtttgc
   126841 agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc ggtgacatta
   126901 tctatcaaag ccaggcaatg gaagacctgc tccagcaggt agatattgtg gcgaagagcg
   126961 acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc gccagagcga
   127021 tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc gctgccatcc
   127081 ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt actggtgcga
   127141 ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt ctcgatgaaa
   127201 ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag gaacgggaga
   127261 ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt gccgccacca
   127321 accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc ttttatcgcc
   127381 tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat atccctcttt
   127441 tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt gacgccatcc
   127501 cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg cgcgagctgg
   127561 aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat ttacatctta
   127621 atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt cgcagttcaa
   127681 tggcgcagtt gctgcacccg acgacgccag agaatgacga agaagaacgt cagcgcattg
   127741 ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg gcgacacgat
   127801 tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc tcggttcgcg
   127861 aggtgttgta atctgctttt gcaggagtat gcatgagaaa caaactctct ttcgacttgc
   127921 agttgagcgc cagaaaagcg gcaatcgctg aacggattgc cgcccataaa attgcccgca
   127981 gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt gtttatggcg atcggattta
   128041 ctttttacct ttccgttatc gccgatgccc cgtcttcata ggcattaacc catctggtgg
   128101 gcggcctttg ctttacactt ggctttattt tgctggcggt ttgcggcacc agcctgttca
   128161 cctcgtcggt aatgacggta atggcaaaaa gtcggggcgt tattagttgg cgaacttggc
   128221 tgattaacgc acttctggtg gcctgcggta atctggcagg tattgcctgt ttcagtttgt
   128281 taatctggtt ttccgggctg gtgatgagtg aaaacgcgat gtggggagtc gcgattttac
   128341 actgcgccga gggcaaaatg catcatacat ttactgaatc tgtcagcctc ggcattatgt
   128401 gcaatctgat ggtttgcctg gcgctgtgga tgagttattg cggacgttcg ttatgcgaca
   128461 aaatcgtcgc catgattttg tccatcaccc tgtttgtcgc cagtggcttt gagcactgta
   128521 tcgccaattt gtttgtgatt ccgttcgcca ttgccattcg ccatttcgcc cctacctcct
   128581 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggtc agccatttta
   128641 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
   128701 gtatttgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
   128761 agttcgtcgg gctgaagatt attcttgcgc gatttgccca tcgggccagg cgtggatcac
   128821 ggctttgatc agcgtcgcca atggaatggc gaagaatacg ccccagaatc cccacaaacc
   128881 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
   128941 caacaccggt actagcaggt tgccgtccag cgcctgaata atcagataca ccgcgaagca
   129001 gctccagaat tcagtacctg cgccaaactg gaatagcgcc acgccaacca ccggaatggt
   129061 caccacaaat gcgccaatgt acggaataag aaccgagaag ccgaccagca ctgccagcag
   129121 cagcgaatag ttcagcccga agagcaagaa ccccagccag gtggcgatcc ccaccacgat
   129181 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
   129241 ctgtcctgcc agcccacggt tacgcggcag cacccggcga acggcgttca gcatctgctc
   129301 tttgtctttc agcaggaaga agaccatcaa tggcaccagc accagataga cggctatggt
   129361 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
   129421 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta acaccggata
   129481 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
   129541 gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
   129601 tataccgaca aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
   129661 ttgcaggcgc acggttggcc attccagcaa ataagccagc acaatagcca ccagcaacgg
   129721 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
   129781 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
   129841 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
   129901 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
   129961 atcttcgccg ttacactcaa aggcggcgag gtgggaacga tatttcacag tatcggtcaa
   130021 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
   130081 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
   130141 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
   130201 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
   130261 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
   130321 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
   130381 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
   130441 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
   130501 tggaagatca acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
   130561 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
   130621 gccaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
   130681 aggtgctgca acgctcggga ttcgatccac aggcgatgcc gaccttcctg gaaaaattgc
   130741 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
   130801 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
   130861 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
   130921 accagctcac cagtaatttg ctggatgaat gggcgaaagg aaacgttctt cagcaacgag
   130981 cggcgcaata tggtcgtgct ttacaggcga tgaaagccaa taaatacgac gaggcgcgta
   131041 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
   131101 ccgatatcga tcttgagcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
   131161 gcgatttacg caccaatccg gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg
   131221 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
   131281 gcaacggctg ggatttgctg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
   131341 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
   131401 tgagtagcgc cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc
   131461 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
   131521 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
   131581 gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtgctcta
   131641 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
   131701 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactaa acctggctga
   131761 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
   131821 gcgcccgatt atggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
   131881 ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag cacattgccg gatgcgacgc
   131941 ttgccacgtc ttatccggcc tacgaatact atggattaac ttcgcgtagg tcaggcgttc
   132001 gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc acaaacggaa
   132061 tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac gtcataaata
   132121 gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct tccggcagtt
   132181 caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca tcagaaagtg
   132241 gctgcaattt gtagatctgc ccccagtcga gacgcgacgc gagatccggt aatcccagat
   132301 tcaactgccg cggtggacga tcgccggtga tcaacaaccg tgttttgccc gattccagaa
   132361 ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca atacactcaa
   132421 tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc ggaacaaacc
   132481 aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac aattccgcgc
   132541 aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga tagatgtaac
   132601 cgctatgttc ctgacgcagc acgttttgca gcgcggccag taaagaggag ttatcccccg
   132661 gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag agctgtgccg
   132721 gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt accacagaat
   132781 cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt gtctgttata
   132841 tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactgat cagtttgaaa
   132901 atcaaactta agccgatacc gacgatggtc gccagcgcca taccttttaa ctccgccgcg
   132961 ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa tcacggaagt caggatcagg
   133021 ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa cacgaatacc ggaagcacca
   133081 atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat catctggata
   133141 gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc cccgccgata
   133201 acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc gtaagtggta
   133261 tttggcgtag agccaaagaa gccggagatc acggtcgaca ggccgttagc aaacatcgaa
   133321 cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt tactaccagg
   133381 tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag aatggcaaac
   133441 cactcgaagc gcggcgtata gagggttggc agtgcaaacc agtgagcatt aataatcggc
   133501 gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac gccaattaaa
   133561 atcgggataa ttgccaggaa accacgaaac agcacggaac ctaaaaccgt gaccgccagg
   133621 gtggtaatag agatgatgat ggttttggag tttggcgttt gcccttcagc cgggagtaaa
   133681 cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac gattgcgccc
   133741 attgccgcag gtggaaacag cacgtccaac cagccggtcc ccgctttctt cacgataaaa
   133801 gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac ttcataccct
   133861 aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag ataagccgga
   133921 attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa taacagtaca
   133981 gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa catggcgaac
   134041 aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact caccccgata
   134101 gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt actcaaaaaa
   134161 aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac caaagatttt
   134221 gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc cctggtcaat
   134281 cgatgcagta tacagttcga cgtccgggtg cgctttttcc agcgcagcga taccttctgg
   134341 cgcagcgacc agcaccagaa ctttgatgct gctgcagccc gcttttttca gcaggtcgat
   134401 ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg ccatacgctc
   134461 atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt cttcattacg
   134521 gtacataccg acaacgctga tgcgcgcgct cggaacgttt tccagcacac cgtccatcat
   134581 accaagaccc gcacgcagaa ttggcacaac ggtaattttc ttacctttga tctggtcgat
   134641 ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt cggcggtcgc
   134701 ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct tggtgctgat
   134761 atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga cttccacgat
   134821 cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgaaggat tatacctcct
   134881 ttcttcaagg cggcaatatt attttcgttg actttagtca aaatgataac agtttgagat
   134941 aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa aactaacctt
   135001 tgccctggca caagtcttct ttcgccgcgc gcccggggaa aagacgtgca aaaaggttgt
   135061 gtaaaacagt ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat tttatttttc
   135121 taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct tagctacaaa
   135181 gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa aggcgtagtg
   135241 aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc gctgtgtgca
   135301 ttgccgcaaa aatatcgtga acccgtgctg gtttccggca ctgacggcgt aggtaccaag
   135361 ctgcgtctgg caatggactt aaaacgtcac gacaccattg gtattgatct ggtcgccatg
   135421 tgcgttaatg acctggtggt gcaaggtgcg gaaccgctgt ttttcctcga ctattacgca
   135481 accggaaaac tggatgttga taccgcttca gcagtgatca gcggcatcgc ggaaggttgt
   135541 ctgcaatcgg gctgttctct ggtgggtggc gaaacggcag aaatgccggg gatgtatcac
   135601 ggtgaagatt acgatgtcgc gggtttctgc gtgggcgtgg tagaaaaatc agaaatcatc
   135661 gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag cggtccgcac
   135721 tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc
   135781 accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg catttacgtg
   135841 aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc
   135901 ggcggcttct gggaaaacat tccgcgcgta ttgccagata atacccaggc agtgattgat
   135961 gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggcagg taacgttgag
   136021 cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc cctgcctgct
   136081 ccggaagtgg acaaagccct cgccctgcta aatgccaacg gtgaaaacgc gtggaaaatc
   136141 ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg aatattgtgg
   136201 tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt aaaaccaaca
   136261 aaattaaagg caccgtacgg gcagttttca gcaataaggc cgacgcgttc ggccttgaac
   136321 gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt gacagtcgtg
   136381 aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat gtggtcgtgc
   136441 tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc gggcgtttgc
   136501 tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat cgtcaggcgc
   136561 tggaaaatgg cgatgaagag cacggtacat cggtgcattt cgtcaccgat gaactggacg
   136621 gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg gaagatgaca
   136681 tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt agctggtttg
   136741 ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa cgtctgccgc
   136801 cgcagagcta cgctgccgac gagtaatgcc cccgtagtta aagcgccagc tctgccgctg
   136861 gcgtttttca attcacctgt aaatcgcaag ctccagcagt ttttttcccc cttttctggc
   136921 atagttggac atctgccaat attgctcgcc ataatatcca ggcagtgtcc cgtgaataaa
   136981 acggagtaaa agtggtaatg ggtcaggaaa agctatacat cgaaaaagag ctcagttggt
   137041 tatcgttcaa tgaacgcgtg cttcaggaag cggcggacaa atctaacccg ctgattgaaa
   137101 ggatgcgttt cctggggatc tattccaata accttgatga gttctataaa gtccgcttcg
   137161 ctgaactgaa gcgacgcatc attattagcg aagaacaagg ctccaacgct cattcccgcc
   137221 atttactggg caaaattcag tcccgggtgc tgaaagccga tcaggaattc gacggcctct
   137281 acaacgagct attgctggag atggcgcgca accagatctt cctgattaat gaacgccagc
   137341 tctccgtcaa tcaacaaaac tggctgcgtc attattttaa gcagtatctg cgtcagcaca
   137401 ttacgccgat tttaatcaat cctgacactg acttagtgca gttcctgaaa gatgattaca
   137461 cctatctggc ggtggaaatt atccgtagcg atagcatccg ttacgcgctg ctggagatcc
   137521 catcagataa agtgccgcgc tttgtgaatt taccgccaga agcgccgcgt cgacgcaagc
   137581 cgatgattct tctggataac attctgcgtt actgccttga tgatattttc aaaggcttct
   137641 ttgattatga cgcgctgaat gcctattcaa tgaagatgac ccgcgatgcc gaatacgatt
   137701 tagtgcatga gatggaagcc agcctgatgg agttgatgtc ttccagtctc aagcagcgtt
   137761 taactgctga gccggtgcgt tttgtttatc agcgcgatat gcccaatgcg ctggttgaag
   137821 tgttacgcga aaaactgact atttcccgct acgactccat cgtccccggc ggtcgttatc
   137881 ataattttaa agactttatt aattttccca atgtcggcaa agccaatctg gtgaacaaac
   137941 cactgccgcg tttacgccat atttggtttg ataaagccca gttccgcaat ggttttgatg
   138001 ccattcgcga acgcgatgtg ttgctctatt atccttatca cacctttggg catgtgctgg
   138061 aactgctgcg tcaggcttcg ttcgacccga gcgtactggc gattaaaatt aacatttacc
   138121 gcgtggcgaa agattcacgc atcatcgact cgatgatcca cgccgcacat aacggtaaga
   138181 aagtcactgt ggtggttgag ttacaggcgc gtttcgacga agaagccaac attcactggg
   138241 cgaagcgcct gaccgaagca ggcgtgcacg ttatcttctc tgcgccgggg ctgaaaattc
   138301 acgccaaact gttcctgatt tcacgtaaag aaaacggtga agtggtccgt tacgcacaca
   138361 tcgggaccgg gaactttaac gaaaaaaccg cgcgtcttta tactgactat tcgttgctga
   138421 ccgcagatgc gcgtatcacc aacgaagtac ggcgggtatt taactttatt gaaaacccat
   138481 accgcccggt gacatttgat tatttaatgg tgtcaccgca aaactctcgc cgtctgttat
   138541 atgaaatggt agaccgcgaa atcgccaacg cgcagcaagg gctgcccagt ggtatcaccc
   138601 tgaagctaaa taaccttgtc gataaaggcc tggttgatcg tctgtatgcg gcctccagct
   138661 ccggcgtacc ggttaatctg ctggttcgcg gaatgtgttc gctgatcccc aatctggaag
   138721 gcattagcga caacattcgt gccatcagta ttgttgaccg ttaccttgaa catgaccggg
   138781 tttatatttt tgaaaatggc ggcgataaaa aggtctacct ttcttccgcc gactggatga
   138841 cgcgcaatat tgattatcgt attgaagtgg cgacaccgct gctcgatccg cgcctgaagc
   138901 agcgggtgct ggacatcatc gacatattgt tcagcgatac ggtcaaagca cgttatatcg
   138961 ataaagaact cagtaatcgc tacgttcccc gcggcaatcg ccgcaaagta cgggcgcagt
   139021 tggcgattta cgactacatc aaatcactcg aacaacctga ataaccctat gccaatacac
   139081 gataaatccc ctcgtccgca ggagtttgct gcggtcgatc ttggttcaaa cagttttcac
   139141 atggtcatag cccgtgtggt agatggtgcc atgcagatta ttggccgcct gaaacagcgg
   139201 gtgcatctgg cggacggcct ggggccagat aatatgttga gtgaagaggc aatgacgcgc
   139261 ggtttgaact gtctgtcgct gtttgccgaa cggctacaag ggttttctcc tgccagcgtc
   139321 tgtatagtcg gcacccatac gctgcgtcag gcgctgaacg ccactgactt tctgaaacgc
   139381 gcggaaaagg tcattcccta cccgattgaa attatttccg gtaatgaaga agcccgtctg
   139441 atttttatgg gcgtggaaca tacccaaccg gaaaaaggtc gcaaactggt tattgatatt
   139501 ggcggcggat ctacggaact ggtgattggt gaaaatttcg aacctattct cgttgaaagc
   139561 cgccggatgg gttgtgtcag ctttgcccag ctttattttc ctggcggggt catcaataaa
   139621 gagaattttc agcgcgctcg catggcggca gcacaaaaac tggaaacttt aacctggcaa
   139681 ttccgtattc agggctggaa cgttgcaatg ggcgcttccg gtaccataaa agccgcccat
   139741 gaagtgttaa tggaaatggg cgagaaagac gggataatta ccccggaacg tctggaaaaa
   139801 ctggtaaaag aaattttacg tcaccgtaat ttcgcatcgc tgagtttacc gggtctttcc
   139861 gaagagcgga aaacagtctt cgttcctgga ctggcgattt tatgtggtgt gtttgatgct
   139921 ttagccatcc gtgaactgcg cctttctgac ggggcgttac gcgaaggcgt actgtatgaa
   139981 atggaaggac gtttccgtca tcaggatgtg cgtagtcgca ccgccagcag cctcgccaac
   140041 cagtatcaca tagacagcga acaggcccga cgagtgctgg ataccactat gcaaatgtac
   140101 gaacagtggc gggaacagca accgaagctg gcgcatccgc aactggaggc gctactgcga
   140161 tgggccgcca tgctgcatga ggtcgggttg aatatcaacc acagcggttt gcatcgccac
   140221 tccgcttata ttctgcaaaa cagtgacttg ccgggtttta atcaggaaca gcagctgatg
   140281 atggcgacac tgctgcgcta tcaccgtaaa gcgattaagc tcgacgatct gccgcgcttt
   140341 accttgttta agaagaaaca gttcctgcca ctgatacagc tattgcgcct tggcgtatta
   140401 ctaaacaatc aacgtcaggc aaccaccaca ccgtcaacat tgacactgat tactgatgac
   140461 agtcactgga cactgcgttt cccgcatgac tggtttagtc agaatgcgct ggtactgctt
   140521 gatctggaaa aggagcaaga atactgggaa ggcgtggctg gctggcggtt gaaaattgaa
   140581 gaagaaagta ccccagaaat cgccgcttaa tgctttgcgg gccgacgaga aatgtcggcc
   140641 cgcattattc aggcactttc gcgaatgggt tcgatttcat tcagcgtatc aattaacggt
   140701 tgcggcttac caataagata accctgcata taatcgatcc ccaaagagag caccgcctcg
   140761 cggatctctt cgttttcaac gtactctgcc actacccgca ttttcttcat tcgcgccaga
   140821 tggcaaatcg acgccacaat ctgataatcc agactatttg acacaatatt gcggataaag
   140881 ctgccgtcaa ttttaagcag atcggcattc acatttttaa gccgtgcata gctggcgtag
   140941 ccggtgccga aatcatcaat cgcaatctgg cagcctaatt cctgaagatg ctgcaaggtg
   141001 atttgcgcct gcttaacatt ggtcagagca ttactttcgg tgacttcaaa aataagttgc
   141061 cacgcttcaa tctgatattt agccagcaac tgactgactt caacaggaaa acgagcctga
   141121 cataccgagg ttggagacag attaatagca aaacggtgag cgggcatctt cgctctgttt
   141181 tcagccataa attgcagcgt atgctcaatg acccacatgt cgatactcga cgataaacca
   141241 aattcgtgcg cgaccggcaa gaagctatcg gggccgatca gttcatcatt ctcacctttc
   141301 atgcgcagaa gaatttcatg gtaaacatca ccacgcatac cggtaatcgg ctgggccatc
   141361 aggaaaaaat ggttgtgctc cagcgcctgc tgtagtcgat tcatcatcgc gactttatct
   141421 ttcaattcgc gttgcaaata cattgccccg caacgctgca tattttccgg ggcgttggtc
   141481 acgatggaaa gttcggcgac cgtatttagc tctcccagca gcaggtagat atgattcact
   141541 ggcgagcgca catagcagta actgacgcca atctgcggtt gcatcggcat cccatcccaa
   141601 aagaaacgaa attgcttgag atgactatcc agtgcggtaa tgcgctcctg gtgcgattct
   141661 g
//
LOCUS       DAJTRQ010000002       110949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000002 DAJTRQ010000000
VERSION     DAJTRQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 110949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            359..2644
                     /gene="nrdA"
                     /locus_tag="OO332_000134"
     CDS             359..2644
                     /gene="nrdA"
                     /locus_tag="OO332_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5940751.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2878..4008
                     /gene="nrdB"
                     /locus_tag="OO332_000135"
     CDS             2878..4008
                     /gene="nrdB"
                     /locus_tag="OO332_000135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5940752.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4008..4262
                     /gene="yfaE"
                     /locus_tag="OO332_000136"
     CDS             4008..4262
                     /gene="yfaE"
                     /locus_tag="OO332_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5940753.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4316..4966)
                     /gene="inaA"
                     /locus_tag="OO332_000137"
     CDS             complement(4316..4966)
                     /gene="inaA"
                     /locus_tag="OO332_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5940754.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5130..5372
                     /locus_tag="OO332_000138"
                     /pseudo
     CDS             5130..5372
                     /locus_tag="OO332_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5414..6490)
                     /gene="glpQ"
                     /locus_tag="OO332_000139"
     CDS             complement(5414..6490)
                     /gene="glpQ"
                     /locus_tag="OO332_000139"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5940755.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6495..7853)
                     /gene="glpT"
                     /locus_tag="OO332_000140"
     CDS             complement(6495..7853)
                     /gene="glpT"
                     /locus_tag="OO332_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5940756.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8126..9754
                     /gene="glpA"
                     /locus_tag="OO332_000141"
     CDS             8126..9754
                     /gene="glpA"
                     /locus_tag="OO332_000141"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5940757.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9744..11003
                     /gene="glpB"
                     /locus_tag="OO332_000142"
     CDS             9744..11003
                     /gene="glpB"
                     /locus_tag="OO332_000142"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5940758.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11000..12190
                     /gene="glpC"
                     /locus_tag="OO332_000143"
     CDS             11000..12190
                     /gene="glpC"
                     /locus_tag="OO332_000143"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5940759.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12383..13282
                     /locus_tag="OO332_000144"
     CDS             12383..13282
                     /locus_tag="OO332_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5940760.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13286..13522
                     /locus_tag="OO332_000145"
                     /pseudo
     CDS             13286..13522
                     /locus_tag="OO332_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13563..14033)
                     /locus_tag="OO332_000146"
     CDS             complement(13563..14033)
                     /locus_tag="OO332_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5940761.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14088..15290)
                     /locus_tag="OO332_000147"
     CDS             complement(14088..15290)
                     /locus_tag="OO332_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5940762.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15377..15919)
                     /locus_tag="OO332_000148"
     CDS             complement(15377..15919)
                     /locus_tag="OO332_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940763.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16198..16623
                     /gene="nudI"
                     /locus_tag="OO332_000149"
     CDS             16198..16623
                     /gene="nudI"
                     /locus_tag="OO332_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5940764.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16662..17264)
                     /locus_tag="OO332_000150"
     CDS             complement(16662..17264)
                     /locus_tag="OO332_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5940765.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17572..18711
                     /gene="arnB"
                     /locus_tag="OO332_000151"
     CDS             17572..18711
                     /gene="arnB"
                     /locus_tag="OO332_000151"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5940766.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18715..19683
                     /gene="arnC"
                     /locus_tag="OO332_000152"
     CDS             18715..19683
                     /gene="arnC"
                     /locus_tag="OO332_000152"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5940767.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19683..21665
                     /gene="arnA"
                     /locus_tag="OO332_000153"
     CDS             19683..21665
                     /gene="arnA"
                     /locus_tag="OO332_000153"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5940768.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21662..22552
                     /locus_tag="OO332_000154"
     CDS             21662..22552
                     /locus_tag="OO332_000154"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5940769.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22552..24204
                     /gene="arnT"
                     /locus_tag="OO332_000155"
     CDS             22552..24204
                     /gene="arnT"
                     /locus_tag="OO332_000155"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5940770.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24201..24536
                     /gene="arnE"
                     /locus_tag="OO332_000156"
     CDS             24201..24536
                     /gene="arnE"
                     /locus_tag="OO332_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5940771.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24536..24922
                     /gene="arnF"
                     /locus_tag="OO332_000157"
     CDS             24536..24922
                     /gene="arnF"
                     /locus_tag="OO332_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5940772.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24916..25188)
                     /gene="pmrD"
                     /locus_tag="OO332_000158"
     CDS             complement(24916..25188)
                     /gene="pmrD"
                     /locus_tag="OO332_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5940773.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25293..26648)
                     /gene="menE"
                     /locus_tag="OO332_000159"
     CDS             complement(25293..26648)
                     /gene="menE"
                     /locus_tag="OO332_000159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5940774.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26645..27607)
                     /gene="menC"
                     /locus_tag="OO332_000160"
     CDS             complement(26645..27607)
                     /gene="menC"
                     /locus_tag="OO332_000160"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5940775.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27607..28464)
                     /gene="menB"
                     /locus_tag="OO332_000161"
     CDS             complement(27607..28464)
                     /gene="menB"
                     /locus_tag="OO332_000161"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5940776.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28479..29237)
                     /gene="menH"
                     /locus_tag="OO332_000162"
     CDS             complement(28479..29237)
                     /gene="menH"
                     /locus_tag="OO332_000162"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5940777.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29234..30904)
                     /gene="menD"
                     /locus_tag="OO332_000163"
     CDS             complement(29234..30904)
                     /gene="menD"
                     /locus_tag="OO332_000163"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5940778.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30993..32288)
                     /gene="menF"
                     /locus_tag="OO332_000164"
     CDS             complement(30993..32288)
                     /gene="menF"
                     /locus_tag="OO332_000164"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5940779.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32367..32672)
                     /gene="elaB"
                     /locus_tag="OO332_000165"
     CDS             complement(32367..32672)
                     /gene="elaB"
                     /locus_tag="OO332_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5940780.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32727..33188)
                     /locus_tag="OO332_000166"
     CDS             complement(32727..33188)
                     /locus_tag="OO332_000166"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5940781.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33253..34170
                     /gene="rbn"
                     /locus_tag="OO332_000167"
     CDS             33253..34170
                     /gene="rbn"
                     /locus_tag="OO332_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5940782.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34358..35579
                     /gene="elaD"
                     /locus_tag="OO332_000168"
                     /pseudo
     CDS             34358..35579
                     /gene="elaD"
                     /locus_tag="OO332_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            35999..36614
                     /locus_tag="OO332_000169"
                     /pseudo
     CDS             35999..36614
                     /locus_tag="OO332_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36717..37220
                     /locus_tag="OO332_000170"
     CDS             36717..37220
                     /locus_tag="OO332_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR5940783.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37287..38744)
                     /gene="nuoN"
                     /locus_tag="OO332_000171"
     CDS             complement(37287..38744)
                     /gene="nuoN"
                     /locus_tag="OO332_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5940784.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38751..40280)
                     /gene="nuoM"
                     /locus_tag="OO332_000172"
     CDS             complement(38751..40280)
                     /gene="nuoM"
                     /locus_tag="OO332_000172"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5940785.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40444..42285)
                     /gene="nuoL"
                     /locus_tag="OO332_000173"
     CDS             complement(40444..42285)
                     /gene="nuoL"
                     /locus_tag="OO332_000173"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5940786.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42282..42584)
                     /gene="nuoK"
                     /locus_tag="OO332_000174"
     CDS             complement(42282..42584)
                     /gene="nuoK"
                     /locus_tag="OO332_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5940787.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42581..43135)
                     /gene="nuoJ"
                     /locus_tag="OO332_000175"
     CDS             complement(42581..43135)
                     /gene="nuoJ"
                     /locus_tag="OO332_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5940788.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(43147..43689)
                     /gene="nuoI"
                     /locus_tag="OO332_000176"
     CDS             complement(43147..43689)
                     /gene="nuoI"
                     /locus_tag="OO332_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5940789.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43704..44681)
                     /gene="nuoH"
                     /locus_tag="OO332_000177"
     CDS             complement(43704..44681)
                     /gene="nuoH"
                     /locus_tag="OO332_000177"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5940790.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44678..47404)
                     /gene="nuoG"
                     /locus_tag="OO332_000178"
     CDS             complement(44678..47404)
                     /gene="nuoG"
                     /locus_tag="OO332_000178"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5940791.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47457..48794)
                     /gene="nuoF"
                     /locus_tag="OO332_000179"
     CDS             complement(47457..48794)
                     /gene="nuoF"
                     /locus_tag="OO332_000179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5940792.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(48791..49291)
                     /gene="nuoE"
                     /locus_tag="OO332_000180"
     CDS             complement(48791..49291)
                     /gene="nuoE"
                     /locus_tag="OO332_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5940793.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49294..51096)
                     /gene="nuoC"
                     /locus_tag="OO332_000181"
     CDS             complement(49294..51096)
                     /gene="nuoC"
                     /locus_tag="OO332_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5940794.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51190..51852)
                     /gene="nuoB"
                     /locus_tag="OO332_000182"
     CDS             complement(51190..51852)
                     /gene="nuoB"
                     /locus_tag="OO332_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5940795.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(51868..52311)
                     /gene="nuoA"
                     /locus_tag="OO332_000183"
     CDS             complement(51868..52311)
                     /gene="nuoA"
                     /locus_tag="OO332_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5940796.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(52942..53880)
                     /gene="lrhA"
                     /locus_tag="OO332_000184"
     CDS             complement(52942..53880)
                     /gene="lrhA"
                     /locus_tag="OO332_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5940797.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            54839..56056
                     /gene="alaA"
                     /locus_tag="OO332_000185"
     CDS             54839..56056
                     /gene="alaA"
                     /locus_tag="OO332_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5940798.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            56140..56739
                     /gene="yfbR"
                     /locus_tag="OO332_000186"
     CDS             56140..56739
                     /gene="yfbR"
                     /locus_tag="OO332_000186"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5940799.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(56798..58630)
                     /locus_tag="OO332_000187"
     CDS             complement(56798..58630)
                     /locus_tag="OO332_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5940800.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58717..59367)
                     /gene="hxpA"
                     /locus_tag="OO332_000188"
     CDS             complement(58717..59367)
                     /gene="hxpA"
                     /locus_tag="OO332_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5940801.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59378..59872)
                     /locus_tag="OO332_000189"
     CDS             complement(59378..59872)
                     /locus_tag="OO332_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5940802.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(59955..60410)
                     /locus_tag="OO332_000190"
     CDS             complement(59955..60410)
                     /locus_tag="OO332_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5940803.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            60748..61950
                     /gene="ackA"
                     /locus_tag="OO332_000191"
     CDS             60748..61950
                     /gene="ackA"
                     /locus_tag="OO332_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5940804.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62025..64169
                     /gene="pta"
                     /locus_tag="OO332_000192"
     CDS             62025..64169
                     /gene="pta"
                     /locus_tag="OO332_000192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5940805.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64359..65879
                     /gene="yfcC"
                     /locus_tag="OO332_000193"
     CDS             64359..65879
                     /gene="yfcC"
                     /locus_tag="OO332_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5940806.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(65912..66454)
                     /gene="yfcD"
                     /locus_tag="OO332_000194"
     CDS             complement(65912..66454)
                     /gene="yfcD"
                     /locus_tag="OO332_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5940807.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66512..67063)
                     /gene="yfcE"
                     /locus_tag="OO332_000195"
     CDS             complement(66512..67063)
                     /gene="yfcE"
                     /locus_tag="OO332_000195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5940808.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67119..67763)
                     /gene="yfcF"
                     /locus_tag="OO332_000196"
     CDS             complement(67119..67763)
                     /gene="yfcF"
                     /locus_tag="OO332_000196"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5940809.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            67899..68546
                     /gene="yfcG"
                     /locus_tag="OO332_000197"
     CDS             67899..68546
                     /gene="yfcG"
                     /locus_tag="OO332_000197"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5940810.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68603..68965
                     /gene="folX"
                     /locus_tag="OO332_000198"
     CDS             68603..68965
                     /gene="folX"
                     /locus_tag="OO332_000198"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5940811.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            68986..69879
                     /locus_tag="OO332_000199"
     CDS             68986..69879
                     /locus_tag="OO332_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5940812.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(69927..70817)
                     /gene="rpnB"
                     /locus_tag="OO332_000200"
     CDS             complement(69927..70817)
                     /gene="rpnB"
                     /locus_tag="OO332_000200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5940813.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(71014..71787)
                     /gene="hisP"
                     /locus_tag="OO332_000201"
     CDS             complement(71014..71787)
                     /gene="hisP"
                     /locus_tag="OO332_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5940814.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(71795..72511)
                     /locus_tag="OO332_000202"
     CDS             complement(71795..72511)
                     /locus_tag="OO332_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5940815.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72508..73194)
                     /gene="hisQ"
                     /locus_tag="OO332_000203"
     CDS             complement(72508..73194)
                     /gene="hisQ"
                     /locus_tag="OO332_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5940816.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73284..74066)
                     /gene="hisJ"
                     /locus_tag="OO332_000204"
     CDS             complement(73284..74066)
                     /gene="hisJ"
                     /locus_tag="OO332_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5940817.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74287..75069)
                     /gene="argT"
                     /locus_tag="OO332_000205"
     CDS             complement(74287..75069)
                     /gene="argT"
                     /locus_tag="OO332_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5940818.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75335..75904)
                     /gene="ubiX"
                     /locus_tag="OO332_000206"
     CDS             complement(75335..75904)
                     /gene="ubiX"
                     /locus_tag="OO332_000206"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5940819.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(75999..77516)
                     /gene="purF"
                     /locus_tag="OO332_000207"
     CDS             complement(75999..77516)
                     /gene="purF"
                     /locus_tag="OO332_000207"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5940820.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77553..78041)
                     /gene="cvpA"
                     /locus_tag="OO332_000208"
     CDS             complement(77553..78041)
                     /gene="cvpA"
                     /locus_tag="OO332_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5940821.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78300..78962)
                     /gene="dedD"
                     /locus_tag="OO332_000209"
     CDS             complement(78300..78962)
                     /gene="dedD"
                     /locus_tag="OO332_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5940822.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(78952..80220)
                     /gene="folC"
                     /locus_tag="OO332_000210"
     CDS             complement(78952..80220)
                     /gene="folC"
                     /locus_tag="OO332_000210"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5940823.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80290..81204)
                     /gene="accD"
                     /locus_tag="OO332_000211"
     CDS             complement(80290..81204)
                     /gene="accD"
                     /locus_tag="OO332_000211"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5940824.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81360..82019)
                     /locus_tag="OO332_000212"
     CDS             complement(81360..82019)
                     /locus_tag="OO332_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5940825.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(82102..82914)
                     /gene="truA"
                     /locus_tag="OO332_000213"
     CDS             complement(82102..82914)
                     /gene="truA"
                     /locus_tag="OO332_000213"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5940826.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(82914..83927)
                     /locus_tag="OO332_000214"
     CDS             complement(82914..83927)
                     /locus_tag="OO332_000214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5940827.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(83993..85129)
                     /gene="pdxB"
                     /locus_tag="OO332_000215"
     CDS             complement(83993..85129)
                     /gene="pdxB"
                     /locus_tag="OO332_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5940828.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85228..86223
                     /gene="flk"
                     /locus_tag="OO332_000216"
     CDS             85228..86223
                     /gene="flk"
                     /locus_tag="OO332_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5940829.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86220..87398)
                     /locus_tag="OO332_000217"
     CDS             complement(86220..87398)
                     /locus_tag="OO332_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5940830.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87673..88893)
                     /gene="fabB"
                     /locus_tag="OO332_000218"
     CDS             complement(87673..88893)
                     /gene="fabB"
                     /locus_tag="OO332_000218"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5940831.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            89052..91058
                     /gene="mnmC"
                     /locus_tag="OO332_000219"
     CDS             89052..91058
                     /gene="mnmC"
                     /locus_tag="OO332_000219"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5940832.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91179..91457)
                     /locus_tag="OO332_000220"
     CDS             complement(91179..91457)
                     /locus_tag="OO332_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5940833.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91491..92039)
                     /gene="epmC"
                     /locus_tag="OO332_000221"
     CDS             complement(91491..92039)
                     /gene="epmC"
                     /locus_tag="OO332_000221"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5940834.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(92039..92848)
                     /locus_tag="OO332_000222"
     CDS             complement(92039..92848)
                     /locus_tag="OO332_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5940835.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(92848..93672)
                     /gene="mepA"
                     /locus_tag="OO332_000223"
     CDS             complement(92848..93672)
                     /gene="mepA"
                     /locus_tag="OO332_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5940836.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93676..94761)
                     /gene="aroC"
                     /locus_tag="OO332_000224"
     CDS             complement(93676..94761)
                     /gene="aroC"
                     /locus_tag="OO332_000224"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5940837.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(94796..95728)
                     /gene="prmB"
                     /locus_tag="OO332_000225"
     CDS             complement(94796..95728)
                     /gene="prmB"
                     /locus_tag="OO332_000225"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5940838.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            95894..96445
                     /gene="smrB"
                     /locus_tag="OO332_000226"
     CDS             95894..96445
                     /gene="smrB"
                     /locus_tag="OO332_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5940839.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96549..97439)
                     /locus_tag="OO332_000227"
     CDS             complement(96549..97439)
                     /locus_tag="OO332_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5940840.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97504..97950)
                     /locus_tag="OO332_000228"
     CDS             complement(97504..97950)
                     /locus_tag="OO332_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5940841.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(97947..98417)
                     /locus_tag="OO332_000229"
     CDS             complement(97947..98417)
                     /locus_tag="OO332_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5940842.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98414..98962)
                     /locus_tag="OO332_000230"
     CDS             complement(98414..98962)
                     /locus_tag="OO332_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5940843.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(98967..99689)
                     /locus_tag="OO332_000231"
     CDS             complement(98967..99689)
                     /locus_tag="OO332_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5940844.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99709..100431)
                     /locus_tag="OO332_000232"
     CDS             complement(99709..100431)
                     /locus_tag="OO332_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5940845.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATFTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(100491..102350)
                     /locus_tag="OO332_000233"
     CDS             complement(100491..102350)
                     /locus_tag="OO332_000233"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR5940846.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(102432..102995)
                     /locus_tag="OO332_000234"
     CDS             complement(102432..102995)
                     /locus_tag="OO332_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5940847.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103679..104164)
                     /gene="sixA"
                     /locus_tag="OO332_000235"
     CDS             complement(103679..104164)
                     /gene="sixA"
                     /locus_tag="OO332_000235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5940848.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104367..106511)
                     /gene="fadJ"
                     /locus_tag="OO332_000236"
     CDS             complement(104367..106511)
                     /gene="fadJ"
                     /locus_tag="OO332_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5940849.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106511..107821)
                     /gene="fadI"
                     /locus_tag="OO332_000237"
     CDS             complement(106511..107821)
                     /gene="fadI"
                     /locus_tag="OO332_000237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5940850.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(108001..108285)
                     /locus_tag="OO332_000238"
     CDS             complement(108001..108285)
                     /locus_tag="OO332_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5940851.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108657..109997
                     /gene="fadL"
                     /locus_tag="OO332_000239"
     CDS             108657..109997
                     /gene="fadL"
                     /locus_tag="OO332_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5940852.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110363..>110949
                     /locus_tag="OO332_000240"
     CDS             110363..>110949
                     /locus_tag="OO332_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940853.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGND"
BASE COUNT        27714 a        29750 c        27334 g        26151 t
ORIGIN      
        1 atgactcgtg cttagatcaa tttttgcaat cattagcaaa aagattaata aaccatctat
       61 atcaatttat ccaacctatt ctgccgttca agaaatcgcc gaacagttat ttttaacaaa
      121 tttttctctt cccattgact ttcccggaca ccttgtctga cctaaggtgc gcgaaagcca
      181 ctttttcctt cctgagttat ccacaaagtt atgcacttgc aagagggtca ttttcacact
      241 atcttgcagt gaatcccaaa cataccccct atatatagtg ttctaagcag cttcccttac
      301 tacaggtagt ctgcatgaaa ctattgcgga aagaattcca aaaacaggta cgacatacat
      361 gaatcagaat ctgctggtga caaagcgcga cggtagcaca gagcgcatca atctcgacaa
      421 aatccatcgc gttctggatt gggcggcaga aggactgcat aacgtttcga tttcccaggt
      481 cgagctgcgc tcccacattc agttttatga cggtatcaag acctctgaca tccacgaaac
      541 tattatcaag gctgccgcag acctgatctc ccgtgatgcg ccggattatc agtatctcgc
      601 cgcgcgcctg gcgatcttcc acctgcgtaa aaaagcctac ggccagtttg agccgcctgc
      661 gctgtacgac cacgtggtga aaatggtcga gatgggcaaa tacgataatc atctgctgga
      721 agactacacg gaagaagagt tcaagcagat ggacacgttt atcgatcacg accgtgatat
      781 gaccttctct tatgctgccg ttaagcagct ggaaggcaaa tatctggtac agaaccgcgt
      841 gaccggcgaa atctacgaaa gcgcccagtt cctttatatt cttgttgccg cgtgcctgtt
      901 ctcgaactac ccgcgtgaaa cgcgcctgca atacgtgaag cgtttttacg acgcggtttc
      961 cacatttaaa atttcgctgc cgacgccaat catgtccggc gtgcgtaccc cgactcgtca
     1021 gttcagctcc tgcgtactga tcgagtgcgg tgacagcctg gattccatca acgccacctc
     1081 cagcgcgatt gtgaaatatg tttcccagcg tgccgggatc ggcatcaacg ccggacgtat
     1141 tcgtgcgctg ggcagcccga ttcgcggtgg cgaagcgttc cataccggct gtatcccgtt
     1201 ctacaaacat ttccagacag cggtgaaatc ctgctctcag ggcggtgtgc gcggcggtgc
     1261 ggcaacgctg ttctacccga tgtggcatct ggaagtggaa agcctgctgg tgttgaaaaa
     1321 caaccgtggg gttgaaggca accgcgtgcg tcatatggac tacggggtac aaatcaacaa
     1381 actgatgtat acccgcctgc tgaaaggtga agatatcacc ctgttcagcc cgtccgacgt
     1441 accggggctg tacgacgcgt tcttcgccga tcaggaagag tttgaacgcc tgtataccaa
     1501 atatgagaaa gacgacagca tccgcaagca gcgtgtgaaa gccgttgagc tgttctcgct
     1561 gatgatgcag gaacgtgcgt ctaccggtcg tatctatatt cagaacgttg accactgtaa
     1621 tacccatagt ccgtttgatc cgaccatcgc gccagttcgc cagtctaacc tgtgcctgga
     1681 aattgccctg ccgaccaaac cgctgaacga cgtcaacgac gagaacggtg aaatcgcgct
     1741 gtgtacgctg tctgctttca acctgggcgc aattaatagc ctggatgaac tggaagagct
     1801 ggcgattctg gcggttcgtg cacttgacgc gctactggat tatcaggatt acccgatccc
     1861 ggccgccaaa cgtggagcga tggggcgtcg tacgctgggt attggtgtga ttaacttcgc
     1921 ttactatctg gcgaagcacg gtaaacgcta ctccgacggc agcgccaaca acctgacgca
     1981 taaaaccttc gaagcgattc agtattacct gctgaaagcg tctaatgagc tggctaaaga
     2041 gcaaggcgcg tgcccgtggt ttaacgaaac cacttacgcg aaaggtatcc tgccgatcga
     2101 tacctataag aaagatctgg atgccatcgc taatgagccg ctgcattacg actgggaagc
     2161 attgcgtgaa tcaatcaaaa cgcacggcct gcgtaactcc acgctttctg ccctgatgcc
     2221 gtccgagacc tcttcgcaga tctctaacgc cactaacggt attgaaccgc cgcgcggtta
     2281 cgtcagcatc aaagcgtcga aagacggtat tttgcgccag gtggtgccgg actacgagca
     2341 cctgcacgac gcctatgagc tgctgtggga aatgccgggt aacgatggct acctgcaact
     2401 ggtaggtatc atgcagaaat ttatcgatca gtcgatctct gctaacacca actacgatcc
     2461 gtcacgtttc ccgtcaggaa aagtgccgat gcagcagttg ctgaaagacc tgctcaccgc
     2521 ctacaaattc ggggtcaaaa cgctgtatta tcagaacacc cgtgacggcg ctgaagacgc
     2581 gcaggacgat ctggtaccgt caatccagga cgatggctgc gaaagcggcg catgtaagat
     2641 ctgatattga gatgccggat gcggcgtaaa cgccttatcc ggcctacggc tcggtttgta
     2701 ggcctgataa gacgcgccag cgtcgcatca ggctccgggt gccggatgca gcgtgaacgc
     2761 cttatccggc ctacggctcg gatttgtagg cctgataaga cgcgccagcg tcgcatcagg
     2821 cacaggatgc ggcgtaaaat gccttatccg gcattaaact cccaacagga cacactcatg
     2881 gcatatacca ccttttcaca gacgaaaaat gatcagctca aagaaccgat gttctttggt
     2941 cagccggtca acgtggctcg ctacgatcag caaaaatatg acatcttcga aaagctgatc
     3001 gaaaagcagc tctctttctt ctggcgtccg gaagaagttg acgtctcccg cgaccgtata
     3061 gattaccagg cgctgccgga gcacgaaaaa cacatcttta tcagcaacct gaaatatcag
     3121 acgctgctgg attccattca gggtcgtagc ccgaacgtgg cgctattgcc gcttatttct
     3181 attccggaac tggaaacctg ggtcgaaacc tgggcgttct cagaaacgat tcattcccgt
     3241 tcctatactc atatcattcg taatatcgtt aacgatccgt ctgttgtgtt tgacgatatc
     3301 gtcaccaacg agcagatcca gaaacgtgcg gaagggatct ccagctatta cgatgaactg
     3361 atcgaaatga ccagctactg gcaccttctg ggcgaaggca cccacaccgt taacggtaaa
     3421 accgtaaccg ttagcctgcg cgagctgaag aaaaaactgt atctctgcct gatgagcgtt
     3481 aacgcgctgg aagcgatccg cttctacgtc agctttgctt gttcttttgc ctttgcagaa
     3541 cgcgaactga tggaaggtaa cgccaagatt attcgcctga ttgcccgcga tgaagccctg
     3601 cacctgaccg gtactcagca tatgctgaat ctgctgcgca gcggcgcgga tgatccggag
     3661 atggcggaaa ttgccgaaga atgtaagcag gagtgctatg acctgtttgt ccaggcggca
     3721 cagcaagaga aagactgggc ggattatctg ttccgtgacg gttcgatgat tggtctgaat
     3781 aaagatattc tctgccagta cgttgaatac atcaccaata tccgcatgca ggcagtcggt
     3841 ttggatctgc cgttccagac gcgctccaac ccgatcccgt ggatcaacac ctggctggtg
     3901 tctgataacg tgcaggttgc tccgcaggaa gtggaagtca gttcttatct ggtcgggcag
     3961 attgacgcgg aagtggacac cgacgatttg agtaacttcc agctctgatg gcccgcgtta
     4021 ccctgcgcat cactggcaca caactgctgt gccaggatga acacccttcc cttctggcgg
     4081 cgctggaatc ccacaatgtg gcggttgagt accagtgccg cgaaggttac tgcggctcct
     4141 gtcgcacccg actggtcgca ggtcaggtgg actggattgc cgaaccgtta gcctttattc
     4201 agccggggga aattttgccc tgttgttgcc gggcaaaagg cgatattgaa atcgagttgt
     4261 gatcgggtac tagcgccaga tacgacgctt acgcgtatta tctggcctac aacgattaca
     4321 tggcgtagta atacgctttc acctgctccc agtccgcttt agggatcggc tccagatatt
     4381 tttccagctg gcggaagtca tgattaatcg ctttatcgcg acgcaagcgg cgacgacttt
     4441 tctccagatc aagaaaacca gcttctgcct taccttctgt tttcacatag atatggcgaa
     4501 catagcaaca gccatgctga cgattaacgc tatgcatttt cttaaacgcc agtgccactg
     4561 ctttcaacat ggcttgccgt acttcgtcag aataaggcga tactgcatgc cgggcatacc
     4621 agtcagcaat gctgatgaac cccgccatat cttcagtcac cagcaacgcg cgccattcac
     4681 cctcaatttt caccgcttca ccaaaaacga tcttcggcac gatgacaccg gcccgttcca
     4741 gttctttaat taccgcaacc tcacggacaa tcgttggtcg gccgaacgga taacgtactg
     4801 aatgaaacag atgatgcgtc atgcgcttta catacagctt tttgccgttg cgctcgacgc
     4861 attgcaccct gctcatacca ttacggcgat agttaggctc ttcaacccag tcgccctctg
     4921 ttgcccacca gtgattaaat tcgtcgtact ttgctgaaac tgccataccc tatcgcctgt
     4981 catttttatt aacgacaatg actataggtg gttacctgag caaaatctta atgaaacgtg
     5041 tcgtattccg ctacaggtgt gacaccgctt ttgcccttca tttgcagcgc atagattcca
     5101 ccagccacta ccactaatag tgcgcccagc agataaaatg tgaatttaat cagtcggata
     5161 cagcgatgaa ttttattcga cagggattag gcatcgcctt acaaccagag ttaacgctga
     5221 aaagcattgc aggtgaattg tgttccgttc ctcacgaacc aaccttctat cgacagattt
     5281 cgttgctgac taaagaaaag ccggtagaag gcagtccact gtttttacta caaatgtgca
     5341 tggaacaatt agtggcgatt ggaaaaattt gatatgagac aggatggcgc atgaacgcca
     5401 tcctgcagta acattactct ttattaagaa attttactgc cttatcaggg aaatcagtaa
     5461 acagcccatt tacacccgct ttgttataca gagcatcata taactgattc acatcaggag
     5521 tgtattcagg cagtttatct gaccgcacgg tataaggatg cactaccagt ttattctgct
     5581 gagcatcttg caccatgcca gtgagtttga tattacccgg ctgcgatgtc tcctcaatca
     5641 acatatggta atccggacca ataccatctg catattccgc cacctgtttc atggcacccg
     5701 gcttaaacat ccagtcgtag ttgtaattaa cccagcttcc atccggctgt ttctgctgcg
     5761 tttcattcca gtcggtatag gcaatcagct gtaccagatt gagctccatg cccattttgg
     5821 gttccagctc attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt
     5881 tatcgtcttt accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat
     5941 ccttcccttc ctgatgatgg aaccacggcg ctttgatttc tggatagata ccgatatttt
     6001 tcccggtaga gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgcaccc
     6061 ggaagtcgga cttacccatt gggaaacgcc ccggataagt ctgtactttt ttaccgtttt
     6121 caatatcgaa accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg
     6181 cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac
     6241 gatcgagata atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct
     6301 gctccagata atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg
     6361 gcaaatatcc actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca
     6421 ttgcactgct tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagct
     6481 tcaatttcat gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt
     6541 tcgccaatca tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc
     6601 ataaagccgc catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc
     6661 accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt
     6721 ttcggtgcca gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag
     6781 ccgataacaa tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa
     6841 acgatagtcg cgatggtcac cagtgtcata aagaagacgc cggttgcccc acggttacca
     6901 cggaagactt tgtccgacat ccagccgcac agcagggtgc ccggaatacc cgcatactca
     6961 tagaagaagt aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa
     7021 gtcggtgacc agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg
     7081 atgtaccaca gcagtttgtt cggcagcacg tactgcatga agatttgctt cgcggtcagc
     7141 tcctgttccg ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc
     7201 ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga
     7261 atggcgcaga aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc
     7321 agcaggaaca gcagcggcgg aataccacca ccgacgttgt gcgcacagtt ccacactgac
     7381 acaatgccgc cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc
     7441 caccccatcc cctggaacca accgcagagg aacaacagta caaacatcac cgcaatgctc
     7501 gacgtcgccc atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct
     7561 gcgggcagga aaacgcgcgg attcgagcga tccgataccg aacccatgat gaatttcgaa
     7621 aatccataag caatcgagat ccccgaaagg gcaaaaccta aatcaccgcg tgagaatccc
     7681 tgctcaacca gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca
     7741 tagccaaaga atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc
     7801 tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc
     7861 ctccgtggcc cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt
     7921 ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac
     7981 attattaaat aagatttaca aaatgttcaa aatgacgcat gaaatcacgt ttcactttcg
     8041 aattatgatc gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta
     8101 aaaaacgaac ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat
     8161 tatcattggc ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct
     8221 gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg
     8281 cctgctgcac agcggtgcgc gctatgcagt aaccgatgcg gaatcggccc gcgaatgcat
     8341 tagtgaaaac cagatcctga aacgcattgc acatcactgc gttgaaccaa ccaacggcct
     8401 gtttatcacc ctgccggaag atgacctctc attccaggcc acttttattc gcgcctgcga
     8461 agaagcaggg atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc
     8521 cgttaacccg gcactgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg
     8581 tctgaccgca gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca
     8641 tgaagtcacg gggctgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca
     8701 tctcaccggc gaaactcagg cccttcatgc acctgtcgtg gttaatgccg ctgggatctg
     8761 ggggcaacac attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc
     8821 gctgctgatc atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc
     8881 cgacgccgat attctggtgc ctggcgatac catttcgctg attggtacca cctctttacg
     8941 tattgattac aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct
     9001 gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc
     9061 tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg
     9121 catcgtgttg ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg
     9181 tggcaaactg atgacctatc ggctgatggc tgaatgggct accgacgcgg tatgccgcaa
     9241 actgggcaac acgcgcccct gtacgactgc cgatctggca ctgcctggtt cacaagaacc
     9301 cgctgaagtt accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt
     9361 ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa ggccgtctac accgtagcct
     9421 ggtatgtgag tgcgaagcgg taactgcggg tgaagtgcag tacgcggtag aaaatttaaa
     9481 cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg gggatgggca cctgccaggg
     9541 cgaactctgc gcctgccgcg ctgccggact gctgcaacgg tttaacgtca cgacgtccgc
     9601 gcaatctatc gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat
     9661 cgcctgggga gacgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg
     9721 tggtctggag aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg
     9781 ccggattact ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc
     9841 gtggtcaaag cgcgttgcat ttctcatccg gttcgctgga tttgctgagc catctgccag
     9901 atggtcaacc cgtggcagac attcacagtg gactggaatc tttgcgtcag caggcacctg
     9961 cacatcctta ctctcttctc gggccacaac gcgtgctcga tctcgcttgc caggcgcagg
    10021 cattaatcgc tgaaagcggt gcgcaattgc agggcagcgt agaactggct caccagcgga
    10081 ttacgccgct cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc
    10141 cgctacccgc gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc
    10201 atcttgcggc ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc
    10261 tgccggaact ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc
    10321 gtttccttga taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca
    10381 atacctgcga aatgatcctg atgcccgcct gcttcggcct agccgatgac aaactgtggc
    10441 gttggttgaa tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg
    10501 tgctgggcat tcgtctgcaa aaccagttac agcgccagtt tgtgcgccag ggtggagtgt
    10561 ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct
    10621 ggacccgcaa tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca
    10681 gtttctttag tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc
    10741 ttgatgtgct acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc
    10801 aaccgtggca gcagttcggt gtaaccactg atgagacgct acgcccgtcg caggcagggc
    10861 aaaccattga gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc
    10921 agggatgcgg cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc
    10981 aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt
    11041 ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc
    11101 cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa
    11161 atattgcatc aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga
    11221 tattatccag cgcgcgcggg cgaaatatga caccacgcgc ccgtcgctgc gtaattttgt
    11281 gttgagtcat accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac
    11341 cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg
    11401 tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc
    11461 tcagcaagca caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa
    11521 ccatccgcag ttaggtaaag atttaattaa agtgctcaac gcaatgggta ccggtgtaca
    11581 actgctcagc aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa
    11641 agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg
    11701 cattccggtg attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga
    11761 agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct
    11821 gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt
    11881 ttatcacact ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt
    11941 gcgtaacatc ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg
    12001 tacttacggt tttaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt
    12061 ccgccagata gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg
    12121 gcagattgag atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc
    12181 gctggcttaa actcccttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta
    12241 cacgattccg tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc
    12301 gcaaaggctg aaactggcag atgtcaaagg cctgggataa ccgtaatgtc gcgtcatcat
    12361 aaatatcagg tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt
    12421 atttaaaacc tttatattca cacccgaaac cgcacgggat tttctcgaaa tacatttacc
    12481 agaaccactg cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat
    12541 tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg
    12601 tgacggctat atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt
    12661 tcggccaatg cgctatgcca ctgccgccat gcagcgtcac ctagataaag gctatgacag
    12721 agttccgctg gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc
    12781 acttaactgg ctggatgagt ctgacgatcc gcaacttgcc cggcagttgt acaccgaagc
    12841 ttttccgttg gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat
    12901 agctctgctg gaactgattc aaaagcatat tcgcgaccac gatttaatcg gcatggtcga
    12961 caggatcacc acgcttttgg ttagaggctt cactaatgac agccagctac aaacactgtt
    13021 taattatctg ctgcaatgcg gcgatacctc ccgtttcacc cgttttattc aggagattgc
    13081 cgaacgttca ccactacaaa aggagagatt aatgactatt gctgaacggc tacggcagga
    13141 agggcatcaa attggctggc aggaaggtat gcatgaacaa gccattaaaa ttgccttgcg
    13201 catgctggag cagggctttg aacgtgagat tgtgctggcg gcaacccaac tcactgatgc
    13261 tgatattccg aactgttatt aaaaggagag ggagagattg atgactattg ctgaacggct
    13321 acggcaggaa ggacatcaaa ttggctggca agaaggtaaa ttagtaggtt tgcagcaagg
    13381 taaattagaa ggtttgcagg aaggtatgca tgaacaagcc attaaaattg ctttgcgcat
    13441 gctggaacag ggcattgatc gtgaccaagt gctcgcggcc acccagctaa gcgaagccga
    13501 tctggcagcg aataaccact aattaacaca ggcccacagc cgatccccat gggcctttga
    13561 tatcaataac taccttttat gcgtggccca tttttgcctg atttaaacat cgccagtcgt
    13621 tgatccaggg catcgctgta gagcatcgtg tcaacgccaa cagcgacaaa gttcgctccc
    13681 cacgccaggc attgctgcgc catatcagga gccacagcca gaaaaccagc cgctttaccc
    13741 gcagcacgga tccgccgaat gctggtttca ataattcgct gcacttccgg gtgcccggcg
    13801 ttatccgggt agcccaacga cgcagaaaga tccgctggtc caataaacac gccatcaatc
    13861 ccttcgacgt cgaggatctc gtccaggtta tccagtgccg ttttactttc cacctgcact
    13921 aacagacaaa gcgaatcgtt gacttgcgcc atgtaattct caatgcgtcc ccagcgcgca
    13981 gcccgtgcca cactggcccc aacaccacgc tcaccgtagg gagggttgga caaattttgc
    14041 cggaggggga atgtgccggt gcagtaaacc ggcacagagt ggcgaaatca gacggataaa
    14101 gtcatggact caacaacctc aatccagcca tgctcactag cgatgtcctg gccgtttaac
    14161 caacggcgta gcatattcag tgccatcatt gcgcacactt cctgacggat agccaggctg
    14221 tagcgtgtag tgctgaaacg cacacgcaga gcgaaagtgc cgtctggcgt ggctagcgca
    14281 aagttgagat gctcgttctc gaaacccgaa acagccagtg ccagcccggc aaaatggttg
    14341 gcccgccgtt ctgtaatcca gtgcgcagtt tgcgccaggg tttcttcctg tgaaggaatc
    14401 acttcacacg ccagcaatgg agcacctgcg cgagaaagtt gcaaagccaa taaaccaccg
    14461 gtgaattgct cgctcaacgt caggctgaac tggcggtttt gcaattcgcg actgatctgc
    14521 gcgggcagtc cttcggtgcc ttcgaaaatc acgctctgtc cggcaacacg tttaacgtcc
    14581 agccacagtt tttccatcgc ctgttgctcg cttgccggtc cggtgagttt cagttcgatg
    14641 atcggcattg aggagcgata gcccattgtt acgcccggcg gcagttgtag agtgtccagg
    14701 ctttgtgcca gatcgctttc cgaacgacca aaggtagtta aacgcagaca aaccggcggc
    14761 tgtggtaaag aaaaacgctc gcgcaggcgc ggcaggattt cgtgctcgac catcacctta
    14821 aattctgacg gtacgccggg agtaaagaac atcaggcaac gattaagctg cacggcaaaa
    14881 ccacaggcgg tgcctaccgg gttattgata aattcagcac tggcaggcag ctccgcttgt
    14941 ttacggctgc tcggtgccat tactcgtcca cgttcgtgga aatagcgttc catctctttg
    15001 agccaggctt catgcagcac aaggccttca ccttttgctg tcgcagcggc gagtgcgctt
    15061 aaatcatcgc tggtcggtcc cagcccgccg ttaacgatca gcacatcggc gtgctgacta
    15121 cgttcgcgaa gaatggtgac taagtcatca aggttatccc ccaccgtatt gcggcgagat
    15181 aatggcagcc cctgatgaaa gaaaaaatcg gccagccagg cagcgttagt gtcaacgatt
    15241 tgcccgtata acacttcatc cccggtggat agcatttcca cttttaacat ggttatctcc
    15301 tgcttttgtt atcgaatcac tataacgcaa agggagataa ggcgtagtaa gaaactgaat
    15361 gccgcgccgg aagggatcag aaactggcgt taaccccaac atacaggccg tcagccacag
    15421 cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc gcctcaatac
    15481 tgactggacg cataatggtg taacgcgcgc cagcattcgc ttcctcataa ctttgaatac
    15541 cgctggagag cgaatccgga gagtagtaat actcgccaaa caaacggaag ctgttgccaa
    15601 ttttccactg caaaccacct cctaccgctg cggcataacc ttcatcgccg taattcgggt
    15661 tggtgtacac gccttttccg ccaacggtcg ccattaacgg cccgagaggc agattcaacc
    15721 ccagccccac gcccgcgacg tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaaag
    15781 ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct ttacccgcct
    15841 ggccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg gccagacctg
    15901 ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc
    15961 tgcgggattg tactgcctgg cacagaggat tcaatgagcg aaaaaaggga tctagtcact
    16021 tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac actttaccga
    16081 tacacacaaa aataataata agtgcagtta tcattgcaaa agagattact gtcactttca
    16141 catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa tacccctgtg
    16201 cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa
    16261 atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg cggcgtggag
    16321 tctggcgaac gaattgaaga ggcactacgc cgcgaaattc gcgaagaact gggagaacag
    16381 ctgcttttga cagaaatcac gccgtggacc ttcagcgatg atattcgcac caagacatat
    16441 gcagatggtc gcaaggaaga gatttatatg atttacctga tttttgactg cgtttctgcc
    16501 aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa acctgaagat
    16561 ctggtgcatt atgatttaaa tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt
    16621 taacaatgat gacagccatc actggctgtc aggttgaaaa tttagtggtt aacgaattcc
    16681 ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag ataatcaggt
    16741 ttaaatatcg catcacgctt atttttagca atatatgtca ggcaatgatt atgggtgaaa
    16801 ataaccatat ttttatcagg cgctttgctt tgtaagtcct taattgcact ataaatctca
    16861 ttaccgcact gaagaagtcg tttatctacc gtcagctttt tacccgcgga aaaccaggtc
    16921 gccgactgaa tggtccggac ggtattactg gaataaagat cgaaatcagg gatgtcagcg
    16981 ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat acctgtttta
    17041 tctgacaagc attgattggt tgaacgatcg caacgttcag catggcgaaa caaaacgaca
    17101 accgggtgct gctgtgccag tctggccaat gttttattgt cgatacgtgg caaaccattg
    17161 ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag taaaatgatg
    17221 aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac tccacctgta
    17281 gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga taattagcag
    17341 ttggtgtaat attaaaaatc ctacgatgtc ggcaaaatgc ctcaaatttt tgccaaatgc
    17401 aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat tagtttctta
    17461 aggttaagtt aatattctat ccttaaaatt tcgctccaaa tggcaaaata tacacaacac
    17521 tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa
    17581 tttttgcctt tttcgcgacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt
    17641 ctcgaatccg gttggatcac aaccggtccg aagaatcagg cgcttgagca agccttttgc
    17701 cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc
    17761 acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg
    17821 gtttcaaccc tcaatatgat ttccttgctg ggtgcaacgc cggtaatggt ggatgtcgac
    17881 cgcgatacgc tgatggtcac gcctgaagcg atcgaatcag ccattacgcc acgcactaaa
    17941 gccatcattc cggtgcatta tgccggtgcg ccagcagata ttgacaccat tcgcgccatt
    18001 ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtcgg tacgtattac
    18061 aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat
    18121 attacctgtg ctgaaggtgg cctgattgta actgataatg aaaaccttgc ccgccagcta
    18181 cggatgctga aatttcacgg tctgggtgtc gatgcctatg acagacaaac ctggggccgt
    18241 gcaccgcagg ctgaagtctt aacaccgggc tataagtaca atctgaccga tattaacgcc
    18301 gcgattgccc tgacacagtt agtcaaatta gagcacctca acacccgtcg gcgcgaaatt
    18361 gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc
    18421 tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt
    18481 atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gcattggtac cgggttacat
    18541 ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg
    18601 aataccgaat ggaatagcga acgcatctgt tcgttgccgc tgttcccgga tatgactacc
    18661 gccgatgccg accgcgtcat cacagccctt cagcaactcg caggacaata agccatgttt
    18721 gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc
    18781 ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag
    18841 atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatgctggt cgaagcctca
    18901 caagcggaga acagccatat tgtgtctatt ttgcttaacc gcaattacgg gcaacattca
    18961 gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat
    19021 ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac
    19081 gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag
    19141 atgattaacc ggcttattca gcgcaccact ggcaaagcga tgggtgacta cggttgtatg
    19201 ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga acgcagcacc
    19261 tttatcccga ttctggcgaa tatcttcgcc cgccgtgcaa ttgaaattcc agtacatcat
    19321 gccgagcgtg agtttggtga atccaaatac agttttatgc gcctgattaa tttgatgtac
    19381 gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagtctgct cggcagcatt
    19441 attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga
    19501 ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc
    19561 gctcagttta tcggcatggg attactcggt gaatatatcg gcaggatcta caccgatgtc
    19621 cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa
    19681 taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagctctgc
    19741 tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc ggtgaaaaag
    19801 ccttttatgg ttcggtggct catctggcgg cggaaagaga cattccggtt tatgcgccgg
    19861 ataacgttaa tcatccgctg tgggtggaac gcattgccca actgtcacca gaggtgattt
    19921 tctcttttta ttatcgccat cttatttgcg acgagatttt tcagctcgct cccgcaggtg
    19981 catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg
    20041 tgctggtgaa cggtgaaacg gaaacgggcg ttacattgca ccgaatggta aaacgtgccg
    20101 atgctggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat atcgctatta
    20161 cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca ttacccgcca
    20221 ttaaacacgg taatattctg gaaatcgccc agcgcgaaaa cgaagccacc tgttttggtc
    20281 gcagaacgcc ggatgacagt ttccttgaat ggcataaacc ggcatccgta ctgcacaaca
    20341 tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc aatcagaaat
    20401 tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg gggagcgtga
    20461 tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc gtcaccggac
    20521 aggcgggcga cggcattact atgcagggct cgcaattagc gcagatgctg ggcctggtgc
    20581 aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc cgggtactca
    20641 tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg cgcgaagatc
    20701 attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt ctgaatcatc
    20761 cacattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt gagtatcacg
    20821 tcaaaaaatg tgatttcgtc ttgccgctgg tggcgatagc cacgccgatt gaatataccc
    20881 gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt atccgctact
    20941 gcgttaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat gggatgtgta
    21001 gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg aataaaccac
    21061 gctggattta ttcggtgtca aaacaattac ttgatcgggt gatctgggcc tatggcgaaa
    21121 aagagggttt acagttcacc ctcttccgcc cgtttaactg gatgggacca cgactggata
    21181 accttaatgc agcgcgaatt ggcagctccc gcgctattac gcaactcatt ctcaatctgg
    21241 tagaaggttc accaattaag ctaattgatg gcggaaaaca aaaacgctgc tttactgata
    21301 tccgggacgg tatcgaggcg ttgtaccgca ttatcgaaaa tgcgggaaat cgctgcgacg
    21361 gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa ctgggcgaga
    21421 tgctgctggc gagcttcgaa aaacatccgc tgcgccatca tttcccaccg tttgcgggct
    21481 ttcgcgttgt cgaaagtagc agctactacg gcaaaggata tcaggacgta gagcatcgta
    21541 aaccgagcat ccgcaatgcc caccgctgcc tggactggga gccgaaaatt gatatgcagg
    21601 aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg gataaaccat
    21661 catgaccaaa gtaggcttac gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt
    21721 gccgcgtctg ctggaaatct tgagtaagca taatattcag gccagcattt ttttcagcgt
    21781 cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt ttttgtggaa
    21841 gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg caggtacggc
    21901 ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag cggctaaaca
    21961 tcacgaagtc ggtctgcacg cctgggatca ccatgcctgg caagcccgta gcggtaactg
    22021 ggatcggcaa acaatgatcg acgatattgc acgcggtctt cgcactctgg aagagattat
    22081 cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccaga aggtgatcga
    22141 agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca tgccgttccg
    22201 tccactgctc gaatcaggaa accctggcac tgcgcaaatt ccggtgacct tacccacctg
    22261 ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt tactcaaccg
    22321 catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg aaggctgcgc
    22381 ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg tgacattttg
    22441 ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc gcggaaatat
    22501 tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg
    22561 gtacgttacc ttatcggcat cttcgcgttt attgcctgct attacctgtt accgatcagc
    22621 acgcgtctgc tctggcaacc cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg
    22681 gcatccggcg actggattgt gccccatctg ttagggctac gttatttcga aaaacccatt
    22741 gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa ctttggtgtg
    22801 cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg gtttactctg
    22861 cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct ctcattgttt
    22921 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    22981 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    23041 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    23101 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    23161 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    23221 ctggcgatag cgcagcggga gcctgacttg tggcactatt ttttctgggt tgagcatatt
    23281 caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    23341 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    23401 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    23461 tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt tgcacctctg
    23521 gcaatgctgt tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    23581 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtgatc
    23641 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    23701 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    23761 gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    23821 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    23881 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    23941 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    24001 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    24061 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    24121 gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg
    24181 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    24241 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    24301 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    24361 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    24421 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    24481 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    24541 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    24601 tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    24661 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    24721 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    24781 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    24841 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    24901 cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt cccactcatc
    24961 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    25021 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    25081 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    25141 atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta tcctgtttgc
    25201 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
    25261 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
    25321 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
    25381 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
    25441 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggaa
    25501 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
    25561 tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg
    25621 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
    25681 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
    25741 ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc caggacgtcg
    25801 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
    25861 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
    25921 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
    25981 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
    26041 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
    26101 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
    26161 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc
    26221 gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg tacgccttca
    26281 accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc
    26341 gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg
    26401 ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
    26461 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
    26521 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
    26581 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
    26641 aagatcataa caactgctcc agtacatcag cgtccactaa cggcagtggg ctccccggcc
    26701 agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg
    26761 gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac tcaatggaag
    26821 aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg cgtacttttt
    26881 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    26941 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    27001 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    27061 gataatccgg gttaacatat ttggcaaact gctgaccttt cagcggtgtc caggcgcgat
    27121 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc
    27181 cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg cccggcatat
    27241 cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt
    27301 tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca
    27361 cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    27421 gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg gagatctccc
    27481 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    27541 gcctgtcgcg cagaagcacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    27601 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    27661 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    27721 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag
    27781 cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc
    27841 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg cgtcgtactg
    27901 acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag ccatgtagga
    27961 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    28021 atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc cgatggagta
    28081 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    28141 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    28201 ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga tcacgccgat
    28261 attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    28321 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt
    28381 ttcataacga atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg
    28441 cattgcttca tcaggataaa tcatattgtg tcctttagtc aaaaacgcaa gatctgcgcc
    28501 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    28561 acatggcagt cggcagccag ttccgccgct agggcgcgga atttgctgtc acgttcacca
    28621 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    28681 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    28741 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    28801 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    28861 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgataata
    28921 accccacaaa gccccgccag cccctggcaa gccgccatca tcgccacccg tccgccaagc
    28981 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    29041 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    29101 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    29161 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    29221 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    29281 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    29341 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    29401 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    29461 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    29521 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    29581 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    29641 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    29701 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt
    29761 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg
    29821 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    29881 gtttttctgc cggatgcagc tccagccagt cggcaatatt gccaattaag cgacgtccgc
    29941 gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt
    30001 cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca
    30061 caatttgtgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    30121 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    30181 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    30241 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt
    30301 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    30361 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    30421 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    30481 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    30541 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    30601 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    30661 ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    30721 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    30781 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    30841 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    30901 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    30961 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc
    31021 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    31081 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    31141 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    31201 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    31261 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    31321 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    31381 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    31441 cgctggtttt tatcatccgc catcagccac tctcctaact gctgcgcctg cttatcatca
    31501 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    31561 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    31621 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    31681 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc accttcggcg
    31741 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    31801 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    31861 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcaaccgc
    31921 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    31981 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    32041 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    32101 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    32161 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    32221 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    32281 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    32341 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    32401 ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca
    32461 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt
    32521 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg
    32581 ggctcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca
    32641 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    32701 cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    32761 tgtgggatac catcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    32821 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta
    32881 catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg caacgcttca
    32941 ctgacaatca cccgacctat aacgacaggc tcaagatcat catcactttt cagaatcctc
    33001 gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    33061 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    33121 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    33181 tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa
    33241 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    33301 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    33361 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    33421 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    33481 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    33541 atccgtgaat ttgtggaaac cgcgcagcgg attagcggct catggaccga ttatccgctg
    33601 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    33661 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    33721 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    33781 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    33841 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    33901 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    33961 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    34021 ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc gcgctatgat
    34081 gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg
    34141 gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggtgaaac
    34201 ttgccctgtt atagcaaccc gcgctacata tatccaccca cacaggcata ttgagcgatt
    34261 tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc ctcttttcat
    34321 cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag ttgtcagcaa
    34381 ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac
    34441 cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag aaagtattga
    34501 gttactgcat aatattgcgt taggttatga tgaatttgga aaaaaagctg aagatattct
    34561 ttaccatatt gttagaaacc caacaaatga gaccctatcg attatccgac ttattaaaaa
    34621 tgcctgttta aaattatata atttggcaca taccgcaacc aactcccacc ttaaaccaac
    34681 tggtccggat aattcagatg ttctcctgtt caaaaagcta ttctcccctt cgaaattaat
    34741 gacaattatc ggtaatgaaa ttcctcttat atctgaaaaa cagtcgcttt caaaggtgct
    34801 tttaaatgat gagaataatg aactgagtga tggtacaaac ttctgggata aaaatcgtca
    34861 attaaccaca gatgaaatag cttgctatct tcagaagatc gccgctaatg caaaaaatac
    34921 tcaagtcaat tatcctactg gtctctacgt cccctactcc accagaactc acctggaaga
    34981 cgctctcaat gaaaatatta agagcgatcc atcatggccg aatgaagtcc agttattccc
    35041 cataaatact ggcggacact ggatattagt ttcgctacag aaaatagtaa ataaaaaaaa
    35101 ataataaact acaaataaaa tgcgtcatat tcaactcatt gcgtgcacta ggccatgata
    35161 aagaaaattc acttaagcgt gtcattaata gttttaattc tgaactcatg ggaaaaatgt
    35221 cgaataataa tataaaagtt catttaaatg aaccagagat aatattttta catgccgatc
    35281 ttcagcaata cttaagccaa agttgcggtg cgtttgtgtg catggcagcc caggaagtga
    35341 ttgaacaaag ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg
    35401 acagatttaa aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg
    35461 taaacagaaa ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac
    35521 aattagaaat aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac
    35581 atcattgtta atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct
    35641 aaaaagtcat cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt
    35701 ccagcgtatc gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact
    35761 tttcttaccc tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat
    35821 tatattttta ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca
    35881 ctttttgtat acctctacca agcgtaataa aataaacgct cataattcat tataaataat
    35941 aatgaaataa cgccttttat tgataattta catatgaaaa aaattttcct tttgttatat
    36001 tatttctgtt ttcactccct ctgattattt ttaccaaccc tgggtcactg cattatcacc
    36061 aacgccccga cacgtaagtc ctgagcaatt agaaaaaaca gttcgttatc ttacacaaac
    36121 tgtgcatcca cgtaatgccg gcaatattga taatctgaat aggtccgcag agtatataaa
    36181 agagttcttt gtcagtagcg gtgccagagt taccgcgcag ggcgtccccg attggctatt
    36241 acgactctgc gcctggaagc cagaattacc cttacccggc aatgtcctgg ctttatcccg
    36301 atcagggaga ctttattgct gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg
    36361 taaaagcggc attgttgtca tcccgggatt tatctgttta ttctatgaat gccccagggt
    36421 ttattcccgg tattgatttt tctgatcacc ttaattattg gcaacacgat attcccgccg
    36481 taatgattac tgacaccgct ttttatcgta ataaacaata ccacttgccc ggtgataccg
    36541 cagacagatt gaattatcag aaaatggctc aggtggtaga tggtgttata accttgttat
    36601 acaacagtaa ataataaaaa tttctcgagg cgggtccaga aacagcgaca tgtacatttt
    36661 attttccctc gtctgtttta agatgcgaaa cataaaattc aatggaggaa aaataaatgg
    36721 gaatgattgg ctattttgca gaaattgact cagaaaagat taaccaactg cttgagtcta
    36781 ctgaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc cgactggata
    36841 ttgataaacg ctgggatttt ttacactttg ggctgacagg cacctctgct tttgatcccg
    36901 cgaagaatga tccgctgagt cgtgccgtgc ttgatgaaca cagcctcgaa gatggcatag
    36961 atggcttcct gggattgacc tggaatcagg aattagccgc gacaatcgac cgactggaat
    37021 cactggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat gaaatggaga
    37081 tttatccggg agtgaccttt tcagaagagt tagaggggca actctttgcc tcgatcatgc
    37141 ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc
    37201 tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa aaaccgccga acatgtcggc
    37261 ggctttctga cttacaaact aacagtttac atcagcggca ttgctaaccg cacaatacta
    37321 atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagaaat cagcaccacg
    37381 ataccgcccg cgctgtactg ccagtttgac ggtgcatcgc gacccggttg ttccggtgcg
    37441 tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca
    37501 accacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac
    37561 ttaccgataa agcccagcgt catcgggata ccggccagag acagcatcat caccgtcatc
    37621 actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag ggaatcagca
    37681 tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg
    37741 aacagataac cggccaggta aacccctacc gcttccatcg acatctcgcc ggtttgcagc
    37801 gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc gagcagacgt
    37861 ttgatgttgg tctggctcaa cgccatcagg ttaccgaaga tgatggaggc aaaggcgata
    37921 atcgccagta ctacgcgaat cgcttcgctg tcacccaccg gtgcgtagag gaacagacgc
    37981 atcaccacac cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca
    38041 ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag
    38101 ccgaggccaa caatcatcat gccgaaacct gccagcaaca gcggctcgtt gagcataccg
    38161 tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc cagactgcgc atacaccagc
    38221 gccataccga acagcaggaa agaagacgct gcggcagaaa ggatggtgta tttgatactg
    38281 gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag
    38341 atcagttcaa taccgaggaa cagagacgcc agatggtttg cattcgccag caggatcccg
    38401 cccagcgcgg caattaacac caacaggtag aactcatcct tgttgtcgtt atagccttca
    38461 agccacgggt agtcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa
    38521 agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggcca
    38581 acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg
    38641 aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa gccgacgatc
    38701 agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt
    38761 gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta
    38821 tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag caacaggatc
    38881 ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg gcttttcgct
    38941 ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa cagatgcaaa gaccagccca
    39001 aaggtagaga taacggtaat caccgggaca acctggaagc tgccgaacag aatcataaat
    39061 tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac
    39121 agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta
    39181 tggatacgtt cataaagctg accgcagaga ataaacagac ccgccgccga caagccgtgc
    39241 gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta gattgcaatc
    39301 agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctga
    39361 gcgaaggcca tccacgcacc gtagaagatg ccgataacac ccagccacat agcgattggc
    39421 gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga aacgcaacaa accgtaagcg
    39481 gcggttttca gcaagatccc cgcgaggtcc acggaaccgg cggtcggagc ctgggagtgc
    39541 gcatccggca gccagccatg cagcggaacc accggcattt tgactgcgaa ggcgatgaag
    39601 aagcccagca tcaacagata ttccacacca ctggacattg gcgtattcag cagctcttca
    39661 tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggcg
    39721 atcaacatca ccagaccact cgcctgggtg taaatgaaga acttggttgc caccgtgata
    39781 cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc
    39841 agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata
    39901 acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct
    39961 ttccacgaac atagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc
    40021 gacagaccgt caatggcgag atgaatagag ataccaaaac gcgggatcca cggcatgtcg
    40081 aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac
    40141 cacagttgca gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc
    40201 accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccaataaa gggaattaat
    40261 attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga
    40321 ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcgc
    40381 cgcgaaggca gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt
    40441 aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc
    40501 cacataccag cgcagataac cgttctcgct taacagcaga cctttacctg caaagcggga
    40561 aagaaccgcc gggatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat
    40621 acccaggaac ggcttgacga acactttgtc gtacagccag tcaaagcccc aggcgttgta
    40681 ccaccaggta ctcagcaggc ggcccggcgc actgttggcg atggaggtca ccagagtacg
    40741 tttacccagc cacagccagg ctgccagcag aatgccgacc accgcgacca cgccagaggt
    40801 aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg
    40861 cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg
    40921 caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac
    40981 gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac
    41041 catcagattg atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa
    41101 gcccgcagtg accagcggca gtgccgacag tgctgcgccg cccaccagga agcagagata
    41161 aaccagcgga atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca
    41221 ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat
    41281 caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat
    41341 ctggctcatg gtagagtaag cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa
    41401 accggccagc agcagcgtaa ccgccccgac aatacccacc agatgcagaa cttccggcgt
    41461 catcaggaac aggccgtggg tacgggcgat caggtagaca cccgcggtta ccatcgttgc
    41521 ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg
    41581 caacggcaac tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat
    41641 cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt
    41701 cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac
    41761 acgggtcacg acgaacgctt tcattgctgc tgcgccattc ttcggatcgg tgtaatagaa
    41821 accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca tcagcagcag
    41881 gttatcggcg agaaccagaa ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa
    41941 gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc
    42001 cacaccagtg accaccgaga gcatggtcag cgacaggccg tccagcacca ggttaaaacc
    42061 gatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg cctgctcgcc
    42121 gttagcgaag aaatcaacgc cgataaaggc ggttacctgc gccgccaggc ccacagagcc
    42181 aacgccgacg atcgccgaaa cgttttcaga ccagcgccca cgggagaacg ccagcaggac
    42241 gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt
    42301 actgaatcga tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg
    42361 atactcgctt ctgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc
    42421 tgaccccagt agcttccggc gaccacaaag gccagcgcgg aggcgttaat catgatttcc
    42481 agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa gccaagaacg
    42541 aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt
    42601 ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac
    42661 cgacgtggaa ggccacaacc agacccgcga gcagcagcat agaagccagt tccaccgcca
    42721 gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga
    42781 taccctgatc gttaacaccg aggatggcgt aaacaatcac caccagcatg atggccgaca
    42841 aaattgccgg gccaatccac acctgcggtt tcagccactg gcgttcctgt tcgatttctg
    42901 aaccgcccag gttgagcatc atcaccacga acacgaacag caccataatg gcacccgcgt
    42961 agacgataat ttccagcgca ccggcgaagt aagcgcctag tgagaagaac accccggaaa
    43021 tcgccagcag cgaaataatc aggtacagca gtgcgtgtac cggattggta tgggtgatca
    43081 ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc
    43141 ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc
    43201 cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt
    43261 gcccggaccg gagatcagca gatcctcttt ctcgtaaacc agatcctggc gcttgtattc
    43321 ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag
    43381 accacagaaa atgcagcgtg agaagttaat gcggaaaaat tccggatacc agcgaccgtc
    43441 tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg cacagaggtt
    43501 acaggctacg caacgctctt cgccgtccgg gtcgcgggtc agaacgatac gaccacgata
    43561 acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc
    43621 gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt
    43681 taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc
    43741 agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat tacctggtca
    43801 taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt
    43861 ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc
    43921 gtcaccatca atgcagagat ggtcacaatc ccgatgtact cacccacgaa gaacagaccg
    43981 aacttcatac cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc
    44041 tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga tggcaaaggt aataaaacca
    44101 aagaattgcg ggatgacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg
    44161 aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc
    44221 agggtctgcg cagaagcacg catcgcaccc agcagcgagt atttgttgtt acttgaccag
    44281 cccgcaaaca gcaccgcgta aaccgccaga cctgccatca tcaggaagaa caaaatcccg
    44341 atgttcaggt cggcaaccac ccaacccgga ctgactggca caattgcaaa ggccagcagc
    44401 agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg
    44461 atccagtctt ctttaaagaa cattttgatc atgtccgcaa ccagctggag cgaaccgccc
    44521 cagccaacac ggttaggtcc ataacggttc tggaacagac ccagcaggcg acgttcgcca
    44581 aagctcatga atgccccaca ggtgacaacc accagcagga tccccaccgc tttgaggatg
    44641 gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat
    44701 cctcaagatg cgcgccagcc agcaccggag caatgccgga catacccatc ggcaagccca
    44761 cctgccctgc cgtcagtcct tcggcgattt caaccggcag cgtgaccgta ttgccatcgt
    44821 aactaaagga gacgcgtgta cctgcgttca cacccaactt cgcggcatcc gctgggttga
    44881 gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat
    44941 cgctgccaaa caggtggtag tacggcgcga tacgccattt cccgtcctgc ggctggaagc
    45001 gtgccggtac gctggtgaag taatccagac cgttttcgct ggtttcaaac agacgcacgc
    45061 ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg
    45121 gggagttcca gcccggcgcc caggcaaacg gcacttgcga acggtgcgcg gtcggctggt
    45181 tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat
    45241 gaacgctgat attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca
    45301 gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata cccgccagtt
    45361 ccgggatttt cgccacaaca gcgtcaatta catggtcgag ctgcgtccag tccacttcac
    45421 ggctcagcaa ggtgctgtgc agcgagtgca gccagcgcca gctttccagc atgacagttt
    45481 tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg ttgggcgcga ccttcattat
    45541 taattaccgt accgtcgctt tcagcaaagc tggcagcgga aagcaccaga tgggcgtttt
    45601 ccatgatcgc tgtgcgttga tgatcaacca ccatcaccag cggcgcttta gccagcgcag
    45661 cattcacgcg ggtagcagaa gcgtaacgat gcagatcgtt ttccaacacc accaccgcgt
    45721 cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag cgaaccgcca cccataatgc
    45781 ccagccccat gctgttgacg gaacgggcaa tcatggtgat accaacgtca gcgccgcgac
    45841 ctttcagggc tttcgcgacg ttagccgcag cctgaatcac ctctatgcta ccggcgttcg
    45901 tcccggagat aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg acgtcgattt
    45961 tgttttgcag ctcaggttcg ataccgtcaa ccgctggtgc ggagttatcc agcgcatggg
    46021 cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga
    46081 tatcatccag acgagtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttgac
    46141 cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgca gccatttcgc
    46201 gcgctttacc tttcaccgcc tgacgcactg ccagtgcgac gcgcgcgccg gtctgggtaa
    46261 cgtcttcgcc cagcaccagt accgcatcgt aagattcgat ttcgcgcaga gccggagtat
    46321 aaatgccgcc ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag
    46381 cgataccggt gtagaagttt tcttcgccca ccagttcacg cagcgcaaag ttgctttcca
    46441 cgctggcacg cggagaacca ataccgatca ctttcttcga ctgacgcaga atatctgccg
    46501 cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg
    46561 gctgacgcgg acgatccttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga
    46621 ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac
    46681 gttcaccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact
    46741 gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg
    46801 ggcaaatttc gaccaggtta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac
    46861 cgaagtagac gttgtcgtgc gcgccataaa cgcccagatc tgtaccgtca gcgtaatctt
    46921 tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg
    46981 gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc
    47041 cggtcatcac ggtcatatcc tgaagatggc agttaccgcc ttcttcacat accggacagt
    47101 cgtgcgggtg gttggtcatt aaccactcga ccacactttc acggaactgt ttcgcttctt
    47161 cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc
    47221 gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga
    47281 cgcttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag
    47341 cttccagcag gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag
    47401 ccattagcat gcttccagtt ttctcggtca gggaccgagc gttaatcgaa attcggttac
    47461 cagcgctctt tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc
    47521 tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg taacggctcc
    47581 actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc
    47641 tcaagtgttt cgatatcgcc cggctggcct tcgccacgct ccagcgcacg cagaattttc
    47701 acgctccacg gcagaccgtc gcggcacggc gtacaccagc cgcaggactc acgggcgaaa
    47761 aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc
    47821 agcgccgtac ccagacggct gcccgcttta ccgatacttt cgaattccat cggcagatca
    47881 aggtgcgctt cggtcaggaa gtcagtcccc gcgccgcctg gctgccaggc tttaaatttc
    47941 agaccatcac gcataccacc ggcgtaatct tcgaggatct cgcgtgcggt ggtgccgaac
    48001 ggcagttccc acagtcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca
    48061 gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga
    48121 acgttacaca gggtttcgac gttgttgaca caggtcggtt tgccccatac gccggaggtt
    48181 gccgggaagg ggggtttcga gcgtgggtta gcacgacgcc cttccaggga gttgattaac
    48241 gctgtttctt ccccgcagat gtagcgccct gccccggtat ggacgaacag ttcgaagtcg
    48301 aaacctgttc ccataatgtt tttgccaagc aagcccgctt cagtggcttc ggcaatagcg
    48361 cggcgcagat ttactgccgc ttcgatatat tcgccacgca ggaagatgta gccacggtaa
    48421 gctttcagcg caaacgcgga gatgagcata ccttccacca gcaggtgcgg cagttgctcc
    48481 atcaacaggc ggtctttata ggtgcccggc tccatttcat cggcgttaca cagcaggtaa
    48541 cggatgttca tggattcgtc tttcggcatc aggctccact tcaggccagt cgaaaagccc
    48601 gcgccgccgc gccctttcag accagcgtct tttacctgat taacgatttc gtccggagac
    48661 agcccggtca gcgccttacg cgccccttcg taaccgtttt tgctgcggta ttcgtccagc
    48721 cacactggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg
    48781 ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg
    48841 cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc acagttcccc aggcagcaag
    48901 ttggcagcag cgtaaagcgg ccatcaaatg tcgtttgccc tggtttgatg ttcagctttt
    48961 tctcgagcgc cgcctgaata ccctgatacc cgttgatatg acagaccacg ctgtcgcaat
    49021 aacggatcac atggcgacca accggctggc ggaagatctg actgtagaac gttgccacac
    49081 cttcgacgtc gcttgccgga atacccagca catcggcgat cgcgtggatc gcaccatccg
    49141 gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt
    49201 cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag
    49261 cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa
    49321 atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg
    49381 aatttgctgc aaatgcgcat aactcggcgt gcggatacga gtgcgataac tcatggtgct
    49441 accgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga
    49501 ttcattggca ggcatcaccg gcccccacga cacttgcagg aagtgggtga tcagggtttc
    49561 gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa
    49621 cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc
    49681 ttccactttc agcattacgc gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc
    49741 aaagtcgaag ttttcatagc cagaataagg acgcgcctta cgcacgtcga agtcgatccc
    49801 ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc
    49861 ggcaacgccc tgggtacgac ctttcagaat ggtattttgc agcgccgctt tctcgtaaga
    49921 cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc agacgatccc agccgcgcgg
    49981 caggtcgtgc gctacgccgc caatacggaa ccacgccggg tgcatacgga aacccgtgat
    50041 cgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat
    50101 tgcgccgacg tcctgaataa aggtcgagat gtacagcagg tggctgttga tacggaacag
    50161 ttcggagagc ataacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt
    50221 ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt caatacggtc
    50281 ggtatacgga atgtagctgt gccaggactg gcgctcgccc attttctccg caccacggtg
    50341 gtggtagccg atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat
    50401 acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc
    50461 gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc
    50521 cagatcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta
    50581 atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg
    50641 gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt
    50701 cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag
    50761 catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc
    50821 aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat
    50881 gacgtaaggt ttcggcagtt tctttaagaa atcgccaact tccagtaatt gttcacgctt
    50941 gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa
    51001 acggttgcgc agttcgccaa ttaccggatc atcaagatga tcgcgggtct gccaggcggg
    51061 ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc
    51121 ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaaatcc ctttgccgac
    51181 aggcgcaaat taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg
    51241 cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca
    51301 ggagagcgga cgacgttctt tgccgataga ttcctgcaac agcatcagtg cctgcatgta
    51361 cgcttcagga cgcggcgggc agcccgggat atacacatca accgggatga atttatcgac
    51421 gccctgcaca acggaataaa tatcgtacat accaccagag ttggcacagg cccccattga
    51481 gataacccat tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt
    51541 ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag cacgcaatac
    51601 ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta
    51661 acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat
    51721 gtcattgagc ttgcccataa acacgttttt gttaacttct tgctccagag ggtcggttac
    51781 gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag
    51841 cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc
    51901 cgggttcata cactcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac
    51961 cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac
    52021 ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa
    52081 gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc
    52141 gaccgaatcg ataccggatt caaacggcac gtttttcgac ctcgcgcgtg cgcgaccgcc
    52201 taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat
    52261 agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc
    52321 atcaaaagta gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg
    52381 gggaggaata aaaaaaacct tacaatcact gtagaaattc ttttatacag ctaattgatg
    52441 tggtctttta ctcctttcta taaccttttg tcaactttaa caaaagtttc ttcacattag
    52501 tttacataat atcaacacca ttagcattta atgccctttc accccagatc cttgacgact
    52561 ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt
    52621 ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg
    52681 tgacacgctt ttgttattcc ataacaaaaa cgcagcaaca aatttacgta ttttttaaca
    52741 tcattgtagc aggtgatttt tttcaggcga ttatttgtgc gttcgggacg tgaatctctg
    52801 gtgattgaaa aataaacagt tttgtacgtt ctgcactatg cgaggaagga tttttactaa
    52861 aaaaaagccg ctggggttta aaacaccccc agcggctcgt tttttacact attgtctcag
    52921 gaattatcta tcgtccgtcg attactcgat atccctttca atcaacaatg aatcatcccc
    52981 ttccggagca gacattgggc tgtattgcca cggattatgg tagctctcca tcgcctgata
    53041 gatcacctgg gccagctcat tattactgga cgggtcatag cacagcaaat attcggtatc
    53101 aggtagcggc ggtaagccat ctactccact cagcacacgc aggtccgggc tcatcatctc
    53161 aaccggcctt gccgtcacgc caagaccggc tttcactgcc gcacgaacgg ccggaagcgt
    53221 cgaggcgaca taagccagtc gccatggaat atctgcttta ttaagcgtcg ccagcaccat
    53281 atcgcgaaac ggacttggat catccagcaa tacaagaggg atcggctcgc ctttttgcaa
    53341 tacgtattcc gctgcgcagt accagtgtgt tggcgaggta cgcaggttca atgccttaaa
    53401 cgctgaagga cgatgggtgg ttaccatcaa atccacttcc tgggattcca gcatttctgc
    53461 catataggca ttacgtttaa cgcggacatc cagcgctaat ttcggataaa ccgaactcac
    53521 gcgatttaac aggaaaggta agatcgtatc ggcagattca tctgaagcac cgatagttaa
    53581 cacgccctga agattactga acattaatga tgagcaggcc tcatcattaa aacgcaggat
    53641 tttcctggcg taaccaagaa gttgaatgcc atgttcagtt aacagtttgt tgcgaccgtg
    53701 acgagcgaac agttctttcc caacgagttg ttccagacgc tgcatttgct gacttacggc
    53761 ggactgagta cgacacacag cggcagctgc ggcagcaaaa gtgttcagat cggcaacagc
    53821 aacaaatgtt ctcagcagat cgaggtcgag gttaattatc ggacgatttg cacttatcat
    53881 atattatcac ttactggcgg ctcatactga gctggttaat gctgtgcaca cacaaacaag
    53941 caattccatt tgtaatgtgc ctccctggca gtttcatccg gaacccggac gaaagtaaaa
    54001 atgcatatga gttgcactaa aaaagcgact cacattgttc cgttataatg cctgaagtag
    54061 atcacagaat atatcttcag ggatcgcata tctattaagt tactcactct tttctattta
    54121 tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt gttccactct cgtgcagcat
    54181 cgctggtcat acgcgaacac gtacaaacag caatggtgaa gcggcatcaa gagcagggat
    54241 ccgtcattta tgcgagcatt ttaccccaaa accttttatt tataagggtc attgcgaatt
    54301 atctgatgca aagttatgtt atgttatgtt atgttatgtt atgttatgtt atgttatgtt
    54361 atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa
    54421 ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct
    54481 tatccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat
    54541 atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac
    54601 agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata acattacatg
    54661 taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac aatcgagcct
    54721 tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg ggagtacatt
    54781 gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt caagattcat
    54841 gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc gtggtccggt
    54901 gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac tgaacatcgg
    54961 caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg tgatacgcaa
    55021 cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc gtaaagccat
    55081 catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata tttacatcgg
    55141 caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca gcggggacga
    55201 aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc tttccagcgg
    55261 taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc tcgatgatat
    55321 tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa ataacccaac
    55381 cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac gtcagcataa
    55441 tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg ctgagcatca
    55501 ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac tgtcgaaaac
    55561 gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga aaaaacacgc
    55621 caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc
    55681 tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg aatttattac
    55741 ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca acgatattcc
    55801 gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa tcgacgccaa
    55861 acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt
    55921 tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc gcattgtcac
    55981 gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt tcctttctgg
    56041 ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag tgttatcccg
    56101 ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag ccattttttt
    56161 gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa cgtgcggacg
    56221 gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct ggcagctatc
    56281 aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact ggcgatgtac
    56341 cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata cttcaatgcg
    56401 caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact ggtcgatatg
    56461 gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc atatagcgat
    56521 gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa atgtctggaa
    56581 gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt
    56641 gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc cagcttccat
    56701 ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt ctgcgtcgca
    56761 tcaggcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac agcatcggga
    56821 tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac ttcacaaagt
    56881 cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa gacaccggtg
    56941 tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa ggcgacactc
    57001 ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc gcggtattag
    57061 agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc atatgtggcc
    57121 cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct cccgttttct
    57181 gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg
    57241 atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg caggcgatga
    57301 tagcggcaac aggattagga atttcatttg tcagcattaa cgccaccatt agcaccagac
    57361 agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc tcttccggta
    57421 agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt ttccagttac
    57481 ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa ccttccagcg
    57541 ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga aaaccaattt
    57601 cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct gccatgccca
    57661 catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc agcaattgct
    57721 cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc aaaacgtcac
    57781 gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa cgccgccagc
    57841 gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca tccagccgtt
    57901 gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat
    57961 attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg
    58021 tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc accagcacga
    58081 ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca cgcagcaatt
    58141 cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg ctaatcagcc
    58201 cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc atggcaacgc
    58261 ttaacaccac gggaataaag atagcgacaa cgctggttga actcataaac gcgccaagcc
    58321 ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc gccactttga
    58381 ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg ccaataataa
    58441 acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact tcggggaccg
    58501 tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca
    58561 ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt
    58621 caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc cataaaaaag
    58681 ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt
    58741 tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga cctcattcag
    58801 gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg ctaggccaga
    58861 aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag
    58921 ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt
    58981 tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg ctaccggcat
    59041 ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat tgagtaaggc
    59101 gattgcccca gaaagcgcgg taataccttc ggtttccgtg gcctcgattt gctccagacg
    59161 cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc gcaaagaggt
    59221 gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc catgacgtct
    59281 ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg ttccatcaag
    59341 atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata atttgattaa
    59401 tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta agcatgcgct
    59461 gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg tcaaaatggg
    59521 tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt gcttcagtgg
    59581 cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg tcctgcaaat
    59641 tagaccagga aacatgcaac gcatgataca tctccataat gtcgatgata gtgcggcagg
    59701 tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat ccgtaaccac
    59761 gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg agcatagtca
    59821 tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc attgttgact
    59881 cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg
    59941 taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt gaaggcgcgc
    60001 ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc caacacctgt
    60061 ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc agggggtaat
    60121 ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact taaggcgaac
    60181 agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca gcagagagta
    60241 aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat aacgcgattt
    60301 tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta atgctgtccg
    60361 cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat gcttcacctc
    60421 aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg gacaacgttc
    60481 aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga aggcctaaat
    60541 agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg aaaattacgc
    60601 aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat
    60661 gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg acgttttttt
    60721 agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt tctgaactgc
    60781 ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga gtacctttct
    60841 ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat ggacggcaat
    60901 aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt
    60961 aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat cggtcaccgt
    61021 atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc tgttattcag
    61081 ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct gatcggtatc
    61141 gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt atttgacacc
    61201 gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta caacctgtac
    61261 aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag
    61321 gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg
    61381 ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggt
    61441 ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc
    61501 atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc
    61561 aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac
    61621 aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg
    61681 aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgtattcact
    61741 ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg
    61801 ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc
    61861 atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc
    61921 gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct
    61981 gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta
    62041 tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg
    62101 caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg
    62161 gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg
    62221 ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg
    62281 tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct gaagtcgttc
    62341 tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa
    62401 tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact gacaccccgg
    62461 aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc aaaaacacca
    62521 acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag ggtcgtactc
    62581 gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac aatgttgatc
    62641 cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg tggagctttg
    62701 acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg
    62761 aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc
    62821 acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac cgtcctgacg
    62881 tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc ctgctgctga
    62941 ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg
    63001 gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc ctgcagagct
    63061 tcaacctgga agttccggtt gacgatcacg aacgtatcga gaaagttcag gaatacgttg
    63121 ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag cgcagccgtc
    63181 gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa gcgggcaaac
    63241 gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg
    63301 aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac cgtgttgcag
    63361 cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg
    63421 aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc gaaaccgttg
    63481 cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa caggatgaag
    63541 ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc
    63601 agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc atgctgctgc
    63661 cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc gctgaacagc
    63721 tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg
    63781 ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta gaaaaagttc
    63841 gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac ggtccgctgc
    63901 agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac tctccggttg
    63961 caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag
    64021 cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt atgcgcaaac
    64081 cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc atcgcgctga
    64141 ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt tgcgctgcgg
    64201 atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt tgcattaatc
    64261 ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct cactttgcat
    64321 acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcca
    64381 aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt gttgctattt
    64441 taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag gaagtgcagt
    64501 atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc
    64561 tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acgggcgatg
    64621 aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg aaatacggga
    64681 cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc
    64741 gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc gggaatgaaa
    64801 ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta tttggtatgg
    64861 gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg
    64921 acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt gccagttcgt
    64981 ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg gtgctttctg
    65041 gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca
    65101 tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc
    65161 gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt ggtgactggc
    65221 tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg
    65281 cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca
    65341 tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg
    65401 aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc
    65461 tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc ctcaacagca
    65521 ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg ttgctcttcc
    65581 aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcatta actatgccgt
    65641 tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc
    65701 agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta atggcaacgc
    65761 tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc ctgcttggac
    65821 tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc taccactaaa
    65881 aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc tctacggctt
    65941 catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct ggagtgaact
    66001 catcgcagcg tgcggtgatt tcttccggcg tcagccagca aacttcactg acttcatctt
    66061 cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag acacggcaat
    66121 ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca atgcccaact
    66181 cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga actacaccgc
    66241 ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga cgctggacca
    66301 gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc agacactgtg
    66361 cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca
    66421 cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct ttttctggcg
    66481 ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga
    66541 tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg ccataactcg
    66601 ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa atctcccccc
    66661 gctgttctgc cactggcaga tgggtatgac cgtacaccag cacatcgttc tggtttaaag
    66721 caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt tgtttttcca
    66781 gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga tccacttcgc
    66841 tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc cgttcagcga
    66901 ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc aacacgtcgc
    66961 caaggatcac cagccactgg gcaccgcttt ggacaaacaa ctccacaaca cgttccgtcg
    67021 ccggtaacga cccatgaatg tccgatgcaa acatcagttt catcactcac tcctcgtcga
    67081 aaaaacgtaa gctatgatac tggattctgt cgttgctatc agccagattg cttcgccgaa
    67141 agtgcaataa aacgctggac agacgctcgc tgccactgga atgtcgcata atccaccagg
    67201 cgttctggca cctcatcgcc atgtagtacc aggcggttaa tcattagcgc cagatcagta
    67261 tcagcaatgc accattcacc aaataaattc ggctgaccca gtgctaacaa atgttctgcc
    67321 atcgcgaaca gtttctctgc actggctttt ccctcggccg ttagtggcgc ttttttcgcc
    67381 cccgcaaaga caacatccgt cggacgctct tcgcggatgg gcatcagatc gctgcgcagc
    67441 caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat caagcggata aatacgttcc
    67501 caggtcggtg gcgcaaatcg atcttccaga tactccgcaa tggcagaaga ttcactcaac
    67561 tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc ttgccacgtc
    67621 ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga tatgaaacga caggcctttt
    67681 tcctgcaacg ccacccaggc ggataacaca taaggggaga aaaagtgggc atctgaccaa
    67741 agcgtgatag cgggtttact cattatgtcc tcggcaataa agggggctgg ctatcaaact
    67801 atagagcctt tttgtcactg tcacgtgacg aatactcacg taagtcgccg cgctttctat
    67861 acttgtggtg ttcagatgat taaacaaccg gagctgcaat gatcgatctc tatttcgccc
    67921 cgacacccaa tggtcacaaa attacgctgt ttctcgaaga agcagagctt gattatcgct
    67981 tgattaaggt agacctgggg aaaggcggtc agtttcgtcc ggaatttttg cgcatttcgc
    68041 ccaacaacaa aattccggca attgttgatc attctcctgc cgatggcggc gaaccgttaa
    68101 gcctctttga gtctggtgcc attttgttgt atctggctga gaaaacagga ctctttttga
    68161 gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt attctggcag gtaggcggac
    68221 tggggccgat gcttgggcaa aatcatcatt ttaatcacgc agccccccaa accattcctt
    68281 acgctattga acgttatcag gttgaaactc agcgtcttta ccatgtacta aacaagcggc
    68341 tggaaaactc gccctggctg ggaggcgaga actacagcat tgcggatatt gcctgctggc
    68401 cgtgggttaa tgcctggact cgcctgcgaa ttgacctcgc aatgtatccg gcagtcaaga
    68461 actggcatga gcggatccgt tcgcgccctg ccaccgggca ggcactgcta aaagcacaac
    68521 tcggtgatga gcgttcggat agttaacaga aacaggttct cgtgtattat ttcatcctaa
    68581 gtaaaacaac ggagaacctg caatggcaca acctgccgct attattcgta taaagaacct
    68641 tcgtttgcgt acgtttatcg gaattaagga agaagaaatt aacaaccgtc aggatattgt
    68701 tatcaatgtg acgatccact accccgccga taaagcgcgc actagcgaag atatcaacga
    68761 tgcgctgaat tatcgcaccg taacgaaaaa cattattcag catgtagaga ataaccgttt
    68821 ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac atcactgggt
    68881 gacgtatgct gaagtggaga tcgataaact gcacgcgctg cgctacgccg attcggtatc
    68941 catgacctta agctggcagc gttaatcgcc atatcgggag gctgcatgaa catagtgatc
    69001 accggaggga cgggattaat tggtcgccat ttgattccgc gtttgctgga gctgggccat
    69061 caaattacgg tagtgacgcg taacccgcag aaagccagtt ccgttctcgg ccctcgggtg
    69121 acactatggc aagggcttgc cgatcaaagc aacctcaacg gcgttgatgc ggtaatcaac
    69181 ctggccggag aaccgattgc tgataaacgc tggactcaca agcaaaaaga gcgtctctgc
    69241 caaagccact ggaatatcac gcaaaaactg gtcgatttga ttaatgccag cgacacgccg
    69301 ccgtcggtac tcatttccgg ctcggcaacg ggctattatg gcgacttagg tgaagtggtg
    69361 gttaccgaag aggaaccgcc gcataacgaa tttactcata aactctgcgc ccgctgggaa
    69421 gagattgcct gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg aaccggcgtg
    69481 gtactggcac cggacggcgg tattctcggt aaaatgctgc caccgtttcg tcttggcctg
    69541 ggcgggccga ttggttccgg tcggcagtat ctggcctgga ttcatattga tgatatggtc
    69601 aacggcattc tctggctgct ggataacgag ctgcgcgggc catttaatat ggtttcgccc
    69661 tacccggtac gcaatgaaca atttgcccat gcgctcggtc atgcgctgca tcgcccggct
    69721 attttgcgcg tccccacaac cgccattcgg ctgttaatgg gcgaatcttc agtactggta
    69781 ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc gtttcgctgg
    69841 tacgatttag aagaggcgct ggcggatgtc gttcgctgat gtggtttaca gcaaacatcc
    69901 gccagttaac tccctgtgtt acaggattag tggctttgcg cgataagatc gtctggtgaa
    69961 agtcgggtca ccatcataac taactctctg tctaaacctc tatccagcat ctcctgagca
    70021 atacgcaggg cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa tctgtcagca
    70081 atggtcatca gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc aataaatgca
    70141 cggaaacgct gggcatcccc tgtttgtaat acgtaattaa acagggcttt tagctgtctg
    70201 tcattagtgt tccctgtaac tagcagcgaa acaatttggt cgactaatcc caacagatcg
    70261 cgctgacgaa tatgtttctg aattaactcc aacagcgcca ttttgcggtg ttgcataatc
    70321 tcgtcatccg gcaccacggt aatatccacc aacggaaacg ctgatgaata tattttgcgg
    70381 gctatagcag gctcggcaaa ttcatcaagc cagcagagtg aataaggata aggacttctg
    70441 caaccatgat aaaacagcat cgggatcacc aatggaagct ctttatagcc cgcatcaaga
    70501 tggttttgca ttgccgcaat ggaataacgc atcatgcgaa aagccattaa ttcttccggc
    70561 ttactttggt gctctatcac tacataaata taacccactc cctcctgcgt tttcacagac
    70621 cacaagaggt cggaataata ttgccgcagg tcttcatcaa taaaactgtt tggttctagt
    70681 ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat attaataaaa
    70741 ttccgcgcgg tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg cggcgtggaa
    70801 gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt taagatgacg cgacaataac
    70861 cacgattaac acccaatgca gcaggcagtt tttatctttg gacagcgaat tcagaagtat
    70921 ttttagcgga ctgcaacttt tacatttcag cggaagctga ctcgcaaatt ccccttgaaa
    70981 atactccggc agcgttagcc accggagagg aaattatttc agcgatccct taaggaactg
    71041 ttgcagacga gggctttgcg ggttgccaaa taactgctcc ggcgcgccct cttcttctat
    71101 tttcccctga tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc ccatttcgtg
    71161 agtcactacc accatggttt tcccctcttc tgccagttgc tgcataatac gtaacacttc
    71221 gcctaccagt tcaggatcga gcgccgaggt aggttcatca aacagtaaaa cttctggttc
    71281 catcgccagc gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa gatgcaccgg
    71341 atatttcccc tgcgcacgtt cgtctatccc gactttcgcc agatacttca ccgcccgctc
    71401 gcacgcttcc tgcttgctca ggcccaacac ctgaatcggc gcttccatga cgttttccaa
    71461 caccgtcata tggctccaga gattgaagtg ctggaatacc atcgtcaggc gtgtgcgcag
    71521 taagcgcagt tgatttttat cggcgacttt gagttgaccg tctttgtcgc gcaccagatt
    71581 gatcgtctgg ccattgacca cgatcgatcc ttcactcggt ttttcgagga agttaatgca
    71641 gcgcagaaag gtacttttcc ccgatcccga cgatccgatg atgcttatta catctccggc
    71701 attcgcttgc agtgagaccc ctttcagcac ttcgtgttcg ccatagcgtt tgtgcaaatc
    71761 gataacgttt aatttattct cggacatcgt attctcagtg cgttgaagaa ggtttcacat
    71821 gctgcaacca gcgtttttcc gctctgcgaa agaggctgat caggacataa gagatgatta
    71881 aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg gcgttaatat
    71941 cgcgggctat tttcagcaga tccggcacag tggccgtaaa cgccagcgca gtagagtgca
    72001 gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca gaaggcaaaa
    72061 taatgcagcg atacatttta aaggtcgaga agccataggc tctggcggct tcaatttccc
    72121 catgcggaac cgaacggatt gccccagcaa aaatctcggt agtgtaagcg caggtgttaa
    72181 gcgtcagcgc cagcacggta cagttcaggc cactgcggaa gaaagcgtta aggaattcgg
    72241 ttcccttaac aatctcaagc gtgtacatgc cggaatagaa caccagcaac tgaacataca
    72301 gcggcgtacc gcgaaaaata taggtaaata accagattgg aaactggatg tatttattac
    72361 tggagacacg accaatcgcc agaaacagcg ccaggactcc gcctatcact accgacaaaa
    72421 taagcagcca cagagtgatc gccacaccag taaagcgata accgtcggtc cacagcagcg
    72481 gtttccagta ttcatgtaag atttcgatca caggtcagcc ctcttcacac ccacggagta
    72541 gcggcgctca aggaacagca gcacaccatt ggaaacggtg gtgaaaacca ggtaaatcac
    72601 gccacagacg atggcgaaat agaacggttc ccaggtactt ttgcctgcca gctgcgtggc
    72661 tttgaccaca tcttccaggc cgagtaacga aaccagtgcg gtagatttga ggatcacctg
    72721 ccagttgttg ccaatgcctg gcagggcgta acgcatcatc gccggaaaca tgatcctccg
    72781 aaacacttgc ccgcgagtaa aaccgaacgc cgtcgccgcc tctatatgcc ctttcggcac
    72841 tgccataaac gcgccacgga acgtttcggt aaaataagca ccgtagataa aaccgagagt
    72901 gataatacca gcgaccatcg gatcgatatc aatctgcccg acgcccatcg cctccgtcac
    72961 catgtttagc gcaatctgca aaccatagaa aatcagcagc atcaacacca agtccggcac
    73021 gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc aaaagccgat tttgcgagag
    73081 cttaccgcca gcgccaatta aaccgatgat tacagcgagc actacagagc tgatagccag
    73141 ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa aacccataca acatgctgcc
    73201 tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc tccggttacc cgaagggagc
    73261 gggctttctt tcaccgatgg cgattaacca ccataaacat caaaatcgaa gtactttttc
    73321 gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt cagtgcctcg
    73381 cgcagttcgt tatcttcttt acgcaggccc atgccggtcc ctacgccaaa cagtttttca
    73441 tctttaacag acggaccacc gaatttgtaa tctttaccga cgggttgttt gaggaaacct
    73501 tcgctggcag cgacctcatc ctggaacgcg gcatcaatac gtccggcagt caggtcagaa
    73561 taaatgttgt cctgcccctg atacgagacg atttcaatgc cttttggtgc ccagtgttca
    73621 ttaccgaacg tctcctgggt ggtgccctgc aatacgccta cccgtttgcc tttcagcgac
    73681 tcgactgtcg gctgaatgtc agaatttttc gccaccacca gacgagaatc ggcagcgtac
    73741 agtttgtcgg tgaaggcaat ttcttgctga cgtttttccg taatggaaag cgatgacatg
    73801 atggcgtcaa tcttcttcgc ttttaaggac gggattaacg catccagcgg gttttcgaca
    73861 aacgtacatt gcgtattgat gcgcttgcac aactccttcg ccagctcgat atcgaaacca
    73921 accagttcgc cttgtgaatt ttttgattca aatggcgcat aggtcgggtc ggtaccgatg
    73981 cggatgtttt gcggaattgc agcaagtgcc gcagttgcgc tggagaaggc cagaaccaga
    74041 gagagcgata gcaccagttt tttcataatg tcctcaactg acagtctttt tatagggaat
    74101 atttacaggt ttgttacact tatcgtgcca taaaattgac catcaaggca aaatatcccg
    74161 ccccgttaga cgtattattt ttcattaaaa atgcttaagt atgcatttca atgcaccacg
    74221 atagtgcaac aaacctggcg tgcaccatga tgatcattcc atcaggtaca gcttcccagc
    74281 gacgtatcag tcaccgtaga cattaaagtc gaaatacttt ttcgccatct tgtcgtaggt
    74341 gccgtcctga cgcagctcgc caagcgcctt attgaaggca gccgtcagtt cagcatcatc
    74401 tttacgtagc cctacaccgg tgccatcacc gaagtatttt ttgtctttta ctgatgagcc
    74461 agcaaaggcg aaatctttac cagcaggttg cttgaggaat ccttcgctgg cagcaacttc
    74521 atcttgtaac gcagcatcca gacgtcctgc agccagatcg gaatagacca aatcctggtt
    74581 ggcataggcc accacatcca cgcctttact acgccaggtc tcgttagcgt aagcttcctg
    74641 ggttgatccc tgcagcacac caacatgttt acctttcagt gaatccagcg ttggctgaat
    74701 cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt cggagaaggc
    74761 aatctcctgc tgacgtttat cggtaatgga aagcgacgaa ataatagcgt cgattttttt
    74821 cgctttcagt gaggggagca gcgcgtcaaa gtcactggca acccaggtac atttcacctg
    74881 catccgtttg cacatctcgt taccgagatc gatatcaaag ccaacaaaat cacctttagc
    74941 atctttcgac gagaacggcg cataggtggt atcggttccg atacgtaccg tctccggtag
    75001 cgccgcatag ctggaagccg ctgcggagag accgactaat aaagacagag cgagaatcga
    75061 cttcttcata cataaccctc aagtgaaaat ggctttttta tgttgtgtat tgtgttgtgt
    75121 gtttgcaggc tctttcatgc aggtcttatg ccatcttgac gacaacagta acattcaacg
    75181 ttaaatatgt taataagacg ttgcattatt gtcctgaagt tgaagatagc aggtatagcg
    75241 gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt tgatcgcggt
    75301 tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc gccctgccag cgggcaaaga
    75361 gatcttcagg aagggttatt gcaaactggt caagaacacg attaaccgtc tgatttatca
    75421 catcatcaag ggattgcggg cgatgataaa acgccggaac gggaggcata atcaccgcac
    75481 cgatttctgc cgcctgagtc attaaacgca gatggcctaa gtgcaatggt gtttcacgca
    75541 cgcagagcac caacgggcga cgctctttca gcaccacatc tgccgcacgg gtcagtaagc
    75601 catcagtata gctatggaca atgccggaca gggttttgat tgaacagggt aaaatcacca
    75661 tccccagcgt ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc gcatcgagcg
    75721 tgacatcagc taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg gataaggtct
    75781 ggcgcgccgc ctggctcatc accagatgcg tttcgatatc tgtgacatcg cgcagaacct
    75841 gtaataagcg cacgccataa atcgcgccgc tggcaccgct gatgcctaca atgagtcgtt
    75901 tcataaatag ttgccctgtc agacttgcag gcagactttg cagtatttcg gcgggagttg
    75961 caagtcaggg tgccagaccg gcaccctcag cgaaggcatc atccttcgtt atgcatttcg
    76021 agattttcca cttcgttctg acgttgcact gctttggcgt catcattacg taacgtatcg
    76081 aggaaatcga ggtagccctg atcaacatct ttggtgacgt agacgccgtt gaacaccgag
    76141 cattcaaact gctggatatc cggattttca gcgcgaacgg cgtcgatcag atcgttcaga
    76201 tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa tttcatcaac ttcgcgaccg
    76261 tgagcgatca gttccgtggc gctcggcata tcaataccat aaacgttcgg gaagcgaatt
    76321 tccggtgccg cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc catctcgata
    76381 atctgctcag aagtggtgcc acggacgatg gagtcgtcga ccagcaggac gtttttatcg
    76441 cggaactcgg cgcggttggc attcagttta cggcgcacgg acttacgacg cagctgctgg
    76501 cccggcatga taaaggtgcg gccaacatag cggtttttaa cgaagccctg gcggtacggt
    76561 ttgcccagaa tacgagcaat ttccagcgcg atatcacacg aggtttctgg gatcgggatc
    76621 accacgtcga tatccagatc ttcccattcg cgggcaattt tctcgcccag tttcgtgccc
    76681 atattcacac gcgcgctgta aacggaaatt ttgtcgataa acgagtccgg gcgggcaaag
    76741 tatacatact caaacaggca cggattgctg accggattgt cagcacattg acgggtaaac
    76801 aactgccctt cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg caggaaatca
    76861 aagcccagcg tatcgagcgc tacgctttcg gaagcgacca tatattctgt acggttctcg
    76921 tcaatatcac gttttcccag taccagcgga cgaatcccgt ttggatcgcg gaaagcaacc
    76981 ataccgtggc cgataatcat cgccacacag gcatacgcgc cgcggattaa gcggtttgtg
    77041 gcagcaatgg cagcgaaaat attgtcggct tccagcgggt agtggcggaa gttgtccagc
    77101 tcgctggcga agatattaag cagaatttcc gagtcggaag tggtgttgat gtggcggcgt
    77161 ttttcttcaa acagtttttt acgcaactcg tgagcgttgg tcagattgcc gttgtgggca
    77221 agcgtaatgc catacgggga gttaacgtaa aacggctgcg cttcagaggc gctggagctg
    77281 ccagccgtag ggtaacgcac atgaccaatg cccatattgc cctgcaaacg ctgcatatgg
    77341 cgagcttcaa atacatcgct caccagcccg ttcgctttac gcaaacggaa gcagttattg
    77401 gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac cgttaaggca
    77461 tcataaatcg actggttaac cggcataaca ccggcgatac cgacaatacc gcacatacgt
    77521 ctttttcctc gttaagccac atctcagagc acttacgctc tgggcaagaa acttgacgag
    77581 ctttgcagat aatcaaaaaa ccatctgatg ataaaactga attgcgggat cagctgtgat
    77641 ttgctccagt cttcactttt cgacaccccg gtaaaggagt cgagaaagaa gagaatggcg
    77701 gcaacaatca acacaccgcg caacgcaccg aaacagacgc ccagcacccg atcggtgcct
    77761 gacaaccccg ttttctccac caactggcct atcacgaagt tcacgatagc accaacgatc
    77821 agggtagcga taaacagtac cgcgatggca atcccatttc gaaccagttc gtcttcaaag
    77881 cccgtaaacc agactgacag gtaagtgtag taatgactgg caacaaagaa agcacaaccc
    77941 catgtcacca gcgataacgc ttcacgaaca aagccgcgga tcaggctaac cagagaggaa
    78001 aaagcaatca ccgcgattat ggcgtaatca atccagacca tatgtgtccc acgattttac
    78061 gccctgtcat cctgttcggg gcgcattcta acagaaaaag aaaacgtttg cgtagggatt
    78121 tccttcccgc gcatcaataa aaatggcgct gaaaaaatat tcaacgccat cgacttttta
    78181 tgcctttgcg gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta tccgacctac
    78241 ggttctaaca ctgcccaagc ctgataagac gcgccagcgt cgcatcaggc aagactgtgt
    78301 taattcggcg tatagcccat taccacgcca cttaagccag aaagttgctt caactcaccc
    78361 agcgaacctt tcagcttatc tttcgaggca tccggcccaa ccagaatacg ggtaatttta
    78421 ccctgcactg gcgtggatgg cgacgtataa acccgataac cggcaccgcg cagcttaccg
    78481 acaatctcat tcactttatc ggcatttttc agcgcaccca gttgcacaac ataggcttta
    78541 cccgttggcg ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg cgcaggtggt
    78601 gcttcaacct ttggtttagg cggctccacc ggtttcggct tcggtggggc gaccggtgca
    78661 ggttccggtt caaactcggt gttattggct gcaatagtgg ccggatcgag cgacggtgct
    78721 gcggcatcac ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt cggcaacgcc
    78781 tgggtggcgg ctggcatcat atcaggctca tcacgatcgc ccgctttcgg caccagcggg
    78841 atagccgcga actcatcctg ataatgtttt ttctgcccgt ccagcagccc tggaagtaca
    78901 atcaccccca gcgccaccag cacgatcgtg cccactaacc gattctgaaa cttacttgcc
    78961 accgcttctc ctcgcgtcaa tcacttccat gacatgtgcg accgtgtgga aagaaccaca
    79021 caccagcacg gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg cctgcgcaac
    79081 gctatcaaat gatttgccgt tacccaaatg ctcaagcagt tgttctgccg tggcaccgcg
    79141 cggcccttcc agtggcgcac aataccagtc atcaaccacg cttttcaacc aggccagagt
    79201 tccggcaata tctttatcat gtagcatacc gataaccgcc agcacgcgcc cgtttttcgg
    79261 tagcgctttc atacgcccgg tgagatattc cgccgcatgt ggattatgcg cgacatcaaa
    79321 aataacgcgt ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg cgctggcaat
    79381 cccgtcgcga atggcatttt cactgacttc cagcccgctg gcacgcagtg ccgccagcgc
    79441 cgttgcggca ttcggttgcg ggacaagcgg caacggcaga ttttccagcg tgccgtgagc
    79501 atcgctaaac gcccagtcat gatcggtgac ggaatagttc cactcaacgc cccgacgttg
    79561 taacagtgca cctttttcct gcgccacatc agcaatggta gaaggcattt ccggctcacc
    79621 gacaattgcc ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa tactttcgcg
    79681 atctggaccc agccagtcgg tatgatccag cgcaatactg gttactaccg cggcatcggc
    79741 atcgacaata ttggttgcgt ccagacgacc gcccagccct acttccagaa tcaccacgtc
    79801 aagttgtgcc tgcttgaaca gccacaacgc cgacagcgta ccgtactcga aataggtcag
    79861 ggaaatatca ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg ccgattccgg
    79921 caattcccgg ccctgcacac gtacgcgctc ggtataacgc accagatgag gcgaactgta
    79981 gacgcccact ttgtaccctg ccgccatcag aatcgactcc agcgtacggc aggtggtgcc
    80041 tttgccattc gtacccgcaa cggtaaacac aaatggcgct ggtttcagga cgccaagacg
    80101 cgccgcgacc tggctcacgc gctcaaggcc gagctcgata gttttactgt gcaggttttc
    80161 cagataagaa agccacgaag ccagaggcga cgcggcttga ggagtgcgtt tgataatcat
    80221 ggtatccgct gattcgttac ggtgagaata gcaaaagggc agagccagtg gccctgccct
    80281 tatcagttat caggcctcag gttcctgatc cggtaccggg ggtaccacta cgccttcacg
    80341 cggcgcttca ggattcggcg ctggcagatt catcaacttc gccagaatgc tcgccagttt
    80401 caggcgcatt tccggacgac ggacgatcat gtcgatcgcg cctttctcga tcaggaattc
    80461 actgcgctgg aatctaggcg gcagtttttc gcgaacggtc tgttcgataa cacgcggacc
    80521 ggcaaagccg attaacgctt taggttcagc gatgttgaga tcgcccagca tggcgaaact
    80581 tgcagaaaca ccgcccatcg tcgggtcggt cagcacggag atgtacggca agccgcgctc
    80641 ctgcattttt gccagtgccg cagaggtttt cgccatctgc atcagcgaca tcagtgcttc
    80701 ctgcatacgt gcgccaccag aggcggagaa gcagatcagc gggcagttat cttcctgcgc
    80761 ctgctcaacg gcacgcacga aacgtgcacc cacaacagac cccattgaac cgcccataaa
    80821 ggcgaactcg aatgccgcag cgacaaccgg cattccatac agagtgcctt tcatcaccac
    80881 cagcgcatct ttttcgccgg tttctttctg cgcagatgcc agacggtctt tatacttctt
    80941 ggagtcacga aacttcagct catctttcgg ctcaagctcg ctacccagct ccacaaggct
    81001 tccttcatct aacaggctat gcaggcgatt acgcgctgtc atacgcatgt gatggtcaca
    81061 cttcggacag acctcaagat tacgttccag ctcagcgcgg tataaaacct gaccgcagct
    81121 atcacactta gtccacaccc cttcaggaat gctcgccttg cgggtgggag taatgttgct
    81181 tttaattcgt tcaatccagc tcattaggga cctttctgtc tgaacctggt tcgatgccag
    81241 ttttatcttt ggggacgcat aatgccattt ttgcccccaa cagaccatga atgttgcaca
    81301 ttaaaacata acagcccgaa actttggata aaaaagtggt cgaaccgcgg agttactttt
    81361 tattttgcgg cgcgtgccgc agcgcgtttg tgacggatta tttcgatgac gcccggcaaa
    81421 atagaaacca caataatccc gacgatcagc agcttaaggt tatcctgaac catcgggatt
    81481 gtaccgaaga aatagcctgc gtaggtaaaa agcagtaccc acaacagtgc gccgatcacg
    81541 ttataagcag cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa cggggcgaac
    81601 gttctgacga tcggcacaaa acgggcgaga ataatcgttt tgccgccatg tttctcataa
    81661 aactgatggg ttttgtcgag ataactgcga cggaaaattt tcgaattggg attactgaat
    81721 aacttttcac cgaacaaccg cccgatagtg taattgaccg cgtcacccac aatcgcggcg
    81781 atcagcatca gcactaccat catatggacg ttgagatcgt tggtttccag tgatgccaac
    81841 gctccggcaa caaacagcaa tgaatcaccc ggtaaaaacg gcattaccac cagaccggtt
    81901 tcacagaaca aaattaagaa caaaatggca taaacccaga cgccgtactc cgcgactagc
    81961 tctgccagat gcacgtcaat gtgcaggata aaatcgatga ggaaataaat caggtccata
    82021 tttgcctatg ccttgtactc gtcatctttc aggctgtaac tacgttggct acagcctgaa
    82081 atgttccgag cattattctc gttagtccgc cagaaatagc gggcccattg gcggttttgg
    82141 aagatcatac cggtcagggt aatccaccgc gaccagatac agcccttccg cttttgccgt
    82201 tgctgccgcc agcgttctgt cctttgccgc cagcaactct gctatccagc tctccggctg
    82261 gttgtgggca ccgacttcca tcaggctgcc gacaatattc ctgaccatat gatgtacaaa
    82321 ggcattcgct ttgatatcaa ccaccacata aggaccgtga cgcgtgacat taatatgcat
    82381 aacgttgcgc cacggggttc gggactggca ctgcaccgca cggaacgagg tgaaatcatt
    82441 ctcgcccagc aagcattgcg cagcccgatg catccgttca gcgtccagcg gttcgtaaaa
    82501 atgggttacc cctttactca gtaccgccgg gcgcagccga tgattgtaga tgatgtagcg
    82561 ataacggcga gccgtggcgc taaatcgggc atgaaaatca tcaggtacag ctttaaccca
    82621 acgcacggcg atgtcaccag gtaaattcgc atttacgccc aaggtccacg ccgcgtcttt
    82681 gcgctgcgcg gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc ctgcgtcagt
    82741 acgcccggcg cagaagacgg tgatgggttc gttcgccacc tgggagagcg ccttttccag
    82801 cttctcctgc acactgcgga cttcattctg ccgttgccag ccgtaatact tactgccgtc
    82861 gtactcaatg cccagcgcaa ttttataaac tggcagttgt tgctggtcgg acattagtac
    82921 agatactcct gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc gccaaagcga
    82981 acgttatcgg ccaccgacca gagctggact tgctccggca taccgtagtc attacgcacg
    83041 cagccaacag aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg gaattcgttc
    83101 tcttcagaga gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc ttctgccgcc
    83161 agcggacgca gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac cggtgcctgg
    83221 acgacgcttg ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac ttcgtcaacg
    83281 atacgacgtt cttcacgcac gctaccttcg ctatccggca acaacggcag catattgaac
    83341 gccagttgac gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag caatttcgca
    83401 ctctgccccg ctaacgcatc gaccgctttt ttgccctggg cggaggctga aatcaggctg
    83461 gtaacactga tacgcgacaa accgccctga tcgattaacg gtttcagtgc cgccagcagc
    83521 tggctggtca gactgtctgg tacggcgatg acattccggt tgcggtaatc ggtcagtaca
    83581 aacgggttta cttccggcac caccagcggt acgtcgggtt cgagagcaaa cagtccgctg
    83641 ctgtcgatca ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc ggtagcttct
    83701 ttgcctgcga caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc ggcatcctgc
    83761 acggtgattg ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt acgtgccagt
    83821 gcataaattt ccccaaccgg gaactgacgt tcagccagcg tttcaagcag ggcttcgccc
    83881 atagcgccag ttgcgcccag gacggcaatg ttccagcctt cagacatggt ggtttactcc
    83941 agaaatagca aagctccctg ccaatgttac agcagagagc atgaagaaga gattaacgtg
    84001 ccggatgatg aacggcgtta aaacccagtt tacacagcaa tgatgccgca ctggcgtcat
    84061 cacaaattac atacagagat gaccattcac ggcgctcaag atagtttttg cgcagtttat
    84121 cgaactcacc cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg cgcacatcat
    84181 acaccaaatg caccagcctt ttcagcgtcg gttgatcgag cggaccatgc agcgtaatgc
    84241 ggccaaactc aggcgcgggc agcaacgtat ccagcgcaac gtgttgttca tgcccaataa
    84301 acttgctata ggcttcaaac acttgcgtcg tgccacgcgc tttaccctcc aaggtatagc
    84361 ctgcgatatg cggcgtgccg atatccactt ttgtcagcaa ttccacatta agttccggct
    84421 cgccttccca gacatccagc accacgctta acttctggcc ttcattcagg caggtcagca
    84481 gcgcggtatt atcgacgact gcgccacggc aggcgttaat cagaatcgct ccgggcttca
    84541 ggctacggat cagtttttca tccgccagat gtagcgtttt gtacggacca tctttaaaga
    84601 gtggcgtatg gaaagtcaga atatccgcgc gctggactaa ctcatccagc gagcggaaat
    84661 caccctcatc cccacggtcg gcgcgaggcg gatcgcaaag taaggtttta atccctaacg
    84721 cttccagtcg cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc cccaccgtac
    84781 ggtcgtgcag tgaaaatcca tcgcgttcgg caagcatcag cagggaggag aaaacatatt
    84841 ccaccaccgc aatcgcatta cagccaggtg cagcggaaaa accaattccc gcctgcttca
    84901 accatgcttc atcgacatgg tcggtccccg cagtggcagt tccaacaaat ttaatgggtt
    84961 ttcctgccag caaagattca ttcactttcg tgaccgaacg caccatcagc gcatccgcgt
    85021 ctgccagttg agcgacgggg attggacgcc cgggaaccgc gatcacctca cccaaacggc
    85081 taaataagtc gcgggcataa ggcatatttt catcaacaag gattttcacg tttgtgttac
    85141 ctgtatgaga cgagagttaa ccggacaagt gtgccataat ctcgcggcca ggcatacttg
    85201 cgaagatttc aggtataagg atacgtaatg atacaaccta tttccggccc tcctcttggg
    85261 cagccaccag gtcagggaga taacctaccg tctggcgcgg gcaatcagcc tttatccagt
    85321 cagcaacgta cttcgctgga aagcttaatg acgaaagtga cctcactgac gcaacaacaa
    85381 agagcagaac tgtgggcggg tatcaggcac gatattggtc tgtcgggaga ttcaccgctg
    85441 ctttcgcgtc acttccctgc cgctgagcat aatctggcgc aacgtctgct ggccgcgcaa
    85501 aaaagccatt ctgcccgcca gcttttagcg caattagggg agtatttacg tctggggaat
    85561 aatcgtcagg cggtcacgga ttatatccgt cataactttg gtcagacgcc gctgaatcag
    85621 ctctcaccgg agcaattaaa aaccattctc accctgttgc aggaagggaa gatggttatt
    85681 ccgcaaccac agcagcgcga ggcgaccgac cgtcctttat taccggcgga gcacaatgcg
    85741 ctaaaacagc tggtgaccaa acttgcggcg gcaacggggg aacccagcaa acagatctgg
    85801 caatcgatgc tggaactttc cggggtgaaa gatggcgagt taattccagc gaaactgttt
    85861 aaccatctgg tgacctggct acaggcgcgt cagacgctaa gccagcaaaa tacgccgacg
    85921 ctggaatcac tacagatggc gctaaaacaa cctttagatg ccagtgaact ggcggcgtta
    85981 tcggcatata tccagcaaaa atatggcctt tctgcgcaat catcgctttc ttctgcccag
    86041 gccgaggata ttcttaatca gctttatcaa cggcgggtta aagggattga tccgcgtgat
    86101 atgcaaccgc tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa tatggcaacg
    86161 cgtcccgcgc tgtggatact gttagtcgcg attatcctga tgctggtctg gctggttcgt
    86221 taaccccgac gaaatgacag tatcgtgaca ataataccca gcaccgcaga gatcgccccg
    86281 gcaagaaata ccgaagagta accaaacgtg gtcgccagca ttcccgccag cggcccggag
    86341 acgccgaggg cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc gcgaacttgt
    86401 gaggggacgc gtttaaccac ctccacgccc agcgcaggaa agataagcga acatccggct
    86461 ccggttaacg ccgcgcccgc taatgcgacc catgcacctg gggcttgcca gagcagcaac
    86521 aagcccaccg tttctacaag cagagagaca atcgccactt tcacgccgcc aaaacggtcc
    86581 ggcatccagc caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc ggtaagagta
    86641 aagcccgcca tcgcccatcc tttgctggca aagcagagcg aaacgaaagt cccgataacc
    86701 gcaaaaccaa cgccttgtag tgccagccct aaccctggtt tccagataag cccgacaacg
    86761 ctccacagcg atggacgttc tcccgccagg gccggtactt tgcgcactgt gccgttacag
    86821 gcccacgcca gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa accgtaatgg
    86881 ctatgaatca acaggccaag cggcgcacca acagcgaggg caccgtaaat cgccattccg
    86941 ttccatgaca tcactttgcc agagtgtttt ggccctacga tgcctaaccc ccaggtcaga
    87001 gcgcctgtca gtaactggct ttcaccaaac ccaagaatca aacgcccgat gaccaacagg
    87061 gcaaatttga acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc gccgccagcc
    87121 agaccacaag ctaacatccc ctgaagcgcc gaacgttttg cgccatattg atcggccagt
    87181 cgcccggcgt aaccacgcgt cagcaccgta gccagaaact gaatcccgac ggcaatgccg
    87241 accatggtat tgccatagcc cagatcatga tgaacaaaca gcgggataac cggcaacggc
    87301 aaccctacgg tcatgtaggt gagaaaaacc gcaaaagcga tgcggaagag cgaaaaactg
    87361 gcagaagatc gtgtttcggt ttggcttaca gcagtcatgc attactccag aatgcagcgc
    87421 aaggcgagga gtatccccgt ctcatctctc tggtttcagg gttacggtgc gttggcagga
    87481 tttaacgcgt acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc ctagaactgg
    87541 tggcgattgt caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg ttagtgccgg
    87601 aggccgcatt ggcgcgtaac gtcggatgcg acgctggcgc gtcttatccg acctactccg
    87661 gcctactgcg aattaatctt tcagcttgcg cattaccagc gtggcgttgg tgccgccgaa
    87721 gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg tcacgatgtt
    87781 cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga taaagccgtg
    87841 ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca gtgagtgacc
    87901 ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt cacggatagc
    87961 tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca ggtaatcgat
    88021 tggagtatca acgccatgca tcgccatctg catacaacgt actgcgcctt cgccagacgg
    88081 agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag catagatgta
    88141 agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc cgccgccagc
    88201 gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg gggtgtcgtt
    88261 gtatttagta gacagcgcac ccatcgcgtc gaattcgcag gccatttccc agcacagctc
    88321 ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct ctactgcgtt
    88381 accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa cgccatgaat
    88441 tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag tgaccacata
    88501 cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga acacctggaa
    88561 acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat tctggtaagc
    88621 ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg cataaatgga
    88681 tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat ccagttttat
    88741 gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga aagtgatccc
    88801 tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga tgctggaaac
    88861 aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt cgcacataga
    88921 gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga tcagccattt
    88981 aatagagaaa tttgcgcagc cttacacaca tcgctaagat cgagccaccg cctgtaagac
    89041 gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt aatgctgagg
    89101 gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac gggctggaag
    89161 agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct gagcatccac
    89221 atcctctgtt tgtggtagca gagagcggct tcggcaccgg attaaacttc ctgacgctat
    89281 ggcaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa cgcttacatt
    89341 tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg catcaacact
    89401 ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg cccttgcccg
    89461 gttgccatcg tttattgctc gatgaaggcc gcgtgacgct ggatttatgg tttggcgata
    89521 ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat gcctggtttc
    89581 tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg tttaacgcca
    89641 tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc ggttttgtcc
    89701 gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt gggcgcaaac
    89761 gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc acgccgtggt
    89821 ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt attgccagcg
    89881 cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat tgcgcggatg
    89941 aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg ttattaagca
    90001 aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct cgtcggtttt
    90061 acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag ttaggctggg
    90121 atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc gcagaactgg
    90181 ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca aattgcagcg
    90241 gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt aatgtgctgg
    90301 aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag aatttatccc
    90361 gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca cacagcgtag
    90421 tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact ctcccggtgt
    90481 attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca gagctgaagc
    90541 aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa catcattgta
    90601 ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac gatcagcagc
    90661 agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa gaggttgatg
    90721 tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat ctgccaatgg
    90781 taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg gcggagcaaa
    90841 aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg gctttaggtt
    90901 ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag atgagcgacg
    90961 aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg ttatgggtgc
    91021 ggaaattgtt gaagggtaaa gcggttaagg cgcggtaatc tgctctcgca gtgtttgccg
    91081 gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat aagacgcgat
    91141 aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga cgcctgctga
    91201 aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa ttcaccggca
    91261 ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc ggagtggtca
    91321 tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata cccactggca
    91381 aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag aatgcgtgat
    91441 tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct tcagttgagc
    91501 gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata attttgtagt
    91561 gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc catcacctgc
    91621 gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc cagattgtcg
    91681 caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa cgcctgcggc
    91741 ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc cgggcaatac
    91801 cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca gtgcgaaatc
    91861 tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata cggtacttcc
    91921 gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt attaaattca
    91981 tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt actgttcatt
    92041 agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt aatttggcac
    92101 tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct ttgctcaaca
    92161 ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca aagcccgtcg
    92221 cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt gacgttgcgt
    92281 tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc ataacgaagg
    92341 ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa ccgacgcagc
    92401 caccggcaat taacgcaaag ggtaaaccgt acatccggcg ctggcgatct tcttcaccca
    92461 gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc aaaatctgcc
    92521 gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag ccgacaaagg
    92581 tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga ataaagtaga
    92641 tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc gcgttagcgg
    92701 gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca ccggcaatcg
    92761 agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac agcggagaaa
    92821 ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta gcgcctggca
    92881 ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg gttttggagg
    92941 ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta aaggttgatc
    93001 ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg cgcgatgctg
    93061 gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa ggcaaagttg
    93121 ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg cggcgagttt
    93181 gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac cgtcgcggga
    93241 aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag ttttcggcag
    93301 ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc cgttgaaacg
    93361 cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca gattgctcac
    93421 ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat agcgacgctg
    93481 atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag cgccgacaat
    93541 acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt gggttatttt
    93601 ttgccacggc gtcgctgcca ggctagcgct actggcaagc agagccagca gcgcaatcgc
    93661 ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat tttgcgcccg
    93721 ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga tcggcactgc
    93781 acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt cttcgccaaa
    93841 gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg ccatatgagc
    93901 aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt tgctctggaa
    93961 accctctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta cgtcaaaacc
    94021 gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg cgatgtcggc
    94081 atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg caacaacggt
    94141 gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt catctaacgc
    94201 gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg accagtcttt
    94261 gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa taccaaattt
    94321 ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg tttcgcgggc
    94381 ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt aggtgtaatc
    94441 agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac gctggtcggt
    94501 attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa caccggagag
    94561 tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc cagggcgacg
    94621 acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg gaacaccatc
    94681 gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc gaaagagttg
    94741 tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg tttacttaat
    94801 ctttataaat cgcgaaatgt tctcgtgcgg caagaagctg ctctttagtg agcataaaca
    94861 caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga tattgttcca
    94921 taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca gcaaggtaat
    94981 ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca gatgccaggc
    95041 ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc gcgtcgacat
    95101 acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg aacagatcgg
    95161 aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt tcggcaaccg
    95221 ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag gcataagcac
    95281 aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc ggttgcttgc
    95341 tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc ggcaccagca
    95401 cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc aggtaagcca
    95461 ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt ttttcgctgg
    95521 aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag agcgatggta
    95581 acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac cagatatttg
    95641 ccgcgctgaa gcggcttacc gaccagcgca acatgtcctg aatggtttgc agctcattta
    95701 ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg caataatttc
    95761 ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt gagtgaggaa
    95821 aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga aaattagaaa
    95881 tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg ctctgtttcg
    95941 ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc gaccgcagcg
    96001 taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg atgccagcca
    96061 ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga aatatgttcg
    96121 cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt caccggaatt
    96181 gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg gcgcgttgat
    96241 tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc acgggaagca
    96301 tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga tggcatttca
    96361 ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg aagtggaaga
    96421 gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag cgtaaacgcc
    96481 ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg taggcctgat
    96541 aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg aaaacgacac
    96601 gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc gtgtagtact
    96661 ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc acaaccgtaa
    96721 gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc atcattatca
    96781 gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga aagcgtggtg
    96841 gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac ataattttca
    96901 ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt atgcactgta
    96961 ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga tgcgatccaa
    97021 gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc acaacgaaaa
    97081 attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc agtcagtacc
    97141 aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc gcctttatac
    97201 gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt aacattgaaa
    97261 acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct cacactgtca
    97321 aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac aacactcaga
    97381 acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga aatactcaaa
    97441 ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc tctttgcctg
    97501 taactaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg tgacgcctgt
    97561 tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc cagcgccgga
    97621 tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt ctgttcctgt
    97681 caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt gaaacaccaa
    97741 tggcactcgc tctgactgcc caaggttcat taaatcgctg gcggcaatgg caggaaaatg
    97801 tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga ttaaattgcc
    97861 ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca tcaggctact
    97921 aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt ggcccaggcc
    97981 tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc cgttaacacc
    98041 ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg ccctgcctgt
    98101 ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc aaaatcgctt
    98161 ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc cgtatcgcag
    98221 gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc aataagcaca
    98281 tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg cggctgcact
    98341 aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca cagcaaaata
    98401 agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta aattcagctg
    98461 ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga actcccgagg
    98521 aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga tcactacttt
    98581 tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca ccgttaatca
    98641 cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg ttacaaacaa
    98701 gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg aatacattac
    98761 caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca agcacccgta
    98821 aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca caacatatca
    98881 gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc ccggcctcag
    98941 cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt gaaaaagagc
    99001 ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc catcttcaca
    99061 ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc tgccggattg
    99121 ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg gttattgacg
    99181 gtaaattcct gaccgttgcg ggttaaagta accttatatt gataggggtt tttcatgctg
    99241 acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag tgctatttgc
    99301 agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta gaacaggctt
    99361 tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac ctgcccgttc
    99421 atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact gatcttgtta
    99481 cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc gttattgcgc
    99541 aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg atctggcgtt
    99601 acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt cgtagcaccc
    99661 aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt attggttagc
    99721 tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct gaatgacagg
    99781 ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg gcagtaacaa
    99841 gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat gcgcaacacc
    99901 actccagaac acactcatgt gttcgccagg tttcacccca gccagataaa cattgccgtc
    99961 atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc caaacggagg
   100021 atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga tgacggcaaa
   100081 tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact gggttgcttc
   100141 ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat agttattgac
   100201 gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt taccttctac
   100261 cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca tattctgggt
   100321 acggtgaagt gcgccaccgt gggcagtaaa cgttgcgccg ccctgtaacg acaagcccgc
   100381 agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca gcgaaccatc
   100441 atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt tcaactgata
   100501 gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg tcccactgcc
   100561 atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag cgaaatgtac
   100621 atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga gatgctgaca
   100681 ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt tgtctgctcc
   100741 tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc cgcatcgcgg
   100801 aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg taccattccg
   100861 ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga gaagcgatat
   100921 ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact cacacggaag
   100981 gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt ggtgtgcgag
   101041 tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac accaagcgcc
   101101 gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc gttagcgatc
   101161 ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc ccactcttgc
   101221 ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta tggcatcgag
   101281 gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg aatatgcaac
   101341 gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg aacctgcgtt
   101401 tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg tgccacgccg
   101461 gaaatgtctg gcgcgtagcc gcgcagattg ggaggtaaca tttgatcgtc agtactgaca
   101521 ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc ctcgcccagc
   101581 gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact ccactcccat
   101641 tttttttgag tttcatcgcc gctgaattca tcgtcatcat tactgcgagt atgttgatag
   101701 ttagtctgcc agtcagcacg catacgccac ggccctaggt taaccccaac cgtcccgttg
   101761 ccgctgatct cgttactatc atcaccgcca ttttcttcgt gccgggtttg tgcgttgatg
   101821 ctgtagtccg cgacgatccc ggagatgccg tcatcccaac gtgaaggcgg atcccagtcg
   101881 ggataagtat attcgaggta agcctgtggc agtgaaatga ctaatgcgga ctggcttaaa
   101941 tcagccttaa tttccatgcc ttccagttga ccggatttca ggcatttagc atcgtggctc
   102001 cattgcagat ttttcgccac gtcttctttt aaaccaaact gcgctaccag ttccggtgtc
   102061 agacaagcat agcttttgct cgcgtcatct tcaccagcat accagtaaat atcgtactct
   102121 tccgccaatg gctgtttatt tagttgaacc tgtaaattgt attttccggg ctcaacatat
   102181 ccctggctgg aaaaacgctt cagatcaatt tttgtgtcgc cttttaattc cagaaaacgg
   102241 gaatcaaact gaatgtcgtc ttcagcccag acactgctat aactccctga cattgccagc
   102301 gcaatgcacc acggaagaat ccgtaatcga aaaagagaat ggtcaggcat acacgtttct
   102361 atccgttttt attcttgttc ggtcaaaatc catttaaatc ccccatagca gtgtcgctat
   102421 gggggtatgc cttacaggta agtaatctgg aaggtggtat ttgcttcaaa attacccaga
   102481 tctggcgcat cagctgcgcc gaccagccag gctttaaagt tcagtgtctg cttcgctttg
   102541 cctttgttgg tagaagtgtc tttcactatt ttctggtcaa tacccatgcc gcttttgtaa
   102601 gaggtaccca gagagtcacc gatcgccagg ctaacattgt taaatgccgc accggtatcg
   102661 gtattgaaaa tggtgtaata attgccgcta tttgcagaag aaacggtacc agtaaaggta
   102721 gtggtcattg tttcctgagt atcaaatacg caatcttgca gacgaatctg gaaacttttc
   102781 ggcgtggtgg tgccgttatt tttcagtttt tcggcaccga tatcacccat atcaacttcc
   102841 agtttgtgat cgtccggaac gatagaacac actgagtctt caatggtgcc gtaaaaacgt
   102901 gcagtaccgt tgttacccgc agcgattgtt gccgtagagg tgaacacgcc catcaccatt
   102961 gccgcagcaa tagctgtttt aacaaactta ctcattattt gtccttaaac tataaataat
   103021 taaaaatata aacattgcaa tacattgatt cagtcaatag ccaatgttta tacccagcca
   103081 ttgaatacgg catgcgaccc aaaaagaaca ggtcgtagaa aattgtattc attggtgtgg
   103141 ggccgcatag tgatgtttcc acacccctga tccaatttaa ttttactaaa gagtaaatgt
   103201 agatttaatt aatatattga cagaagaaag gttatcttaa ggacatcctt aaaaaatacg
   103261 gaataagtat ttagaaaaaa tagaaagtat atcaataatg aatatcaaca gcttaaaaaa
   103321 caaacaatga acatataatg cgattggcat ttatccactt ttgacaatat aatatataaa
   103381 cactatagcc agaatttaat caagtttcaa ttacatcctg cctgattatt caactctatt
   103441 ttctctatta tctgtattaa taaatgacac tgagtgtcaa acagacatta ttcatttcaa
   103501 catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa tgaatgtaga
   103561 aagtgtttta tttatagata aggagagtct tcgatagtag tgaggttgac agatatccgc
   103621 tgcctaatga gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaacacatc
   103681 agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat gtaccgtttc
   103741 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
   103801 ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg
   103861 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
   103921 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa tcccctactt
   103981 cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
   104041 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag
   104101 gacgaaccga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
   104161 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
   104221 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
   104281 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag
   104341 cgcggattca tatagctttg accttcttat tgcaggtcag ttgcagttgt tttccaaaaa
   104401 ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac
   104461 tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
   104521 cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcacc aatatccccg
   104581 tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac
   104641 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
   104701 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
   104761 tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
   104821 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
   104881 ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa accaaatttc
   104941 actagcgcgg catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca
   105001 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
   105061 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgccgatgta
   105121 cccgcatgag gaataatctc taccagcggc attttttcca ccggactgaa gaaatgcagg
   105181 ccaataactt gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg taaagatgac
   105241 gtgttcgaag caaaaatggt atgagcggcg caattttgct ccacttccgc caccatctgt
   105301 tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
   105361 cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
   105421 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
   105481 ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc agtgacataa
   105541 gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa tggcgcaggc
   105601 ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat actacgcaaa
   105661 gcttgcgatt gcggcgtcat cgccagttcg ccaaaggcgc gagcttcagc gtcatagccg
   105721 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
   105781 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
   105841 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
   105901 ttcttcgcca gctcaacagc ggcttccagc aaaatggagt gcggaacaac gtcatccacc
   105961 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgagaat catctctaat
   106021 gctgtactga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
   106081 ccaagttgta cttcaggcaa accgagcacc gttttgggat cgtcagtaca aacgcgaccg
   106141 tggcacgcca gcgccagctc cagcccaccg cccaggcaag cgccatgaat agccgcgata
   106201 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagtgct
   106261 tccgcttctt gcgccgtttt gcagttgcca atcatgttga tgtctgctcc ggcgataaaa
   106321 ttgtccggtt tcgcggagat aaacaccacg ccgcgcaact ctttgttttc acggagttgc
   106381 ttaataatgg cgcgcacctg cgaggcaaat tccgccttca gggtattcat tttctcatcc
   106441 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacgctgat
   106501 gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
   106561 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
   106621 tccgcgcgcc ggtcgccgcg aagggatgtc cgtaagcaat cgaaccgcca agcacgttaa
   106681 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca cgagcaaaac
   106741 gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat
   106801 cgatcaatgt cagttcgctc atcgtcaaac cggcacgttc cagcgccagc ggtgttgacc
   106861 aggctggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca
   106921 gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
   106981 ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt taccgttccg tgtttgcgat
   107041 caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt
   107101 cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
   107161 ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
   107221 gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc
   107281 cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag
   107341 agaagagttt caggcgctgg ctcatggttc gagctttgtt gacatcaacc agcacgtgcg
   107401 ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc
   107461 ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc tggaaactgg
   107521 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg gtaccgagaa
   107581 caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagct
   107641 gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
   107701 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
   107761 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagtggtaaa acctgaccca
   107821 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt attaaccatt
   107881 tttttacatt tagccaagcg gagaaaaagg aaagtgggag ctatgacaca gtgaagacag
   107941 aagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgtacgtac ccgagaggaa
   108001 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
   108061 atccaggcgc acaccgtcag actcttcagt gaaagtcggg gagattttgc acggttcgga
   108121 ttccacgcta cgggattttt cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
   108181 tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc
   108241 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
   108301 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
   108361 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
   108421 aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca gctggatcgc
   108481 gtttcttaga tcatatttga aaaaagatgg aaacatactt gcaacattct agctggtccg
   108541 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
   108601 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
   108661 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
   108721 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
   108781 attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga
   108841 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtga
   108901 atatcagcgg aacgtctccg tctggtcgta gcctgcaagc cgataacatc gcgcctacgg
   108961 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
   109021 ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca ggcggctctg
   109081 tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
   109141 ataatgcatg gagctttggt cttggtttca acgccatcta cgctcgcgcg aaaattgaac
   109201 gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc
   109261 aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
   109321 tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac
   109381 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
   109441 gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg
   109501 cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
   109561 tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacacca
   109621 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
   109681 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
   109741 ataactggac tttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc
   109801 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat
   109861 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
   109921 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
   109981 ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgctat gcaggtgact
   110041 ttaactcccc cacttcaccg aaagtagttt cctcattgct tgcctcctga gttttgcaaa
   110101 cagcctgatg gcagcttgcc ctttcaaatc agtaagtggt atccacagac aaacctgaag
   110161 ggaaatgcat attttcaagc gttctgctcg tccttctcaa agagtttact tttctgcatt
   110221 tccaggatac tcaacccccc tggctattgt gcgcttatac actcaaatta aagataggtt
   110281 ctaaataaat gagcgttttt tgatagtcta tttcattagg taatatatat ttgtaacaaa
   110341 tcaatcaaca tagaataaaa tcatgctacc atctatttca atcaacaata ccagcgcagc
   110401 ttacccagaa tccatcaatg aaaataacaa tgatgaaatt aatggattag tacaagagtt
   110461 caaaaacctt tttaatggta aggaaggaat aagcacctgt attaaacatc tacttgagct
   110521 tataaaaaac gccatacgag taaacgacga tccttataga tttaatatta ataattcctc
   110581 agttacttat attgatattg gctccaatga tacagaccat attactattg gtatcgacaa
   110641 ccaagaacca atagaattac ctgcgaacta taaagacaaa gaactcgtcc gtactatcat
   110701 taatgacaac atagttgaga agactcatga tatcaataac aaggaaatga tcttcagcgc
   110761 attaaaagaa atatatgatg gagatcctgg ttttatcttc gataagatat cacacaaact
   110821 cagacatacg gtaacggaat ttgatgagag cgggaaaagc gaaccaacgg acttatttac
   110881 ctggtacggt aaagataaaa aaggcgactc tctcgctatt gtaattaaaa ataaaaacgg
   110941 aaatgatta
//
LOCUS       DAJTRQ010000003        86016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000003 DAJTRQ010000000
VERSION     DAJTRQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..1593)
                     /locus_tag="OO332_000241"
     CDS             complement(<1..1593)
                     /locus_tag="OO332_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5940854.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAE"
     gene            complement(1577..1933)
                     /locus_tag="OO332_000242"
     CDS             complement(1577..1933)
                     /locus_tag="OO332_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5940855.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2056..2238)
                     /locus_tag="OO332_000243"
     CDS             complement(2056..2238)
                     /locus_tag="OO332_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940856.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2222..2662)
                     /locus_tag="OO332_000244"
     CDS             complement(2222..2662)
                     /locus_tag="OO332_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5940857.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2662..2958)
                     /locus_tag="OO332_000245"
     CDS             complement(2662..2958)
                     /locus_tag="OO332_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5940858.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2955..3293)
                     /locus_tag="OO332_000246"
     CDS             complement(2955..3293)
                     /locus_tag="OO332_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5940859.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3290..4501)
                     /locus_tag="OO332_000247"
     CDS             complement(3290..4501)
                     /locus_tag="OO332_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5940860.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4503..5075)
                     /locus_tag="OO332_000248"
     CDS             complement(4503..5075)
                     /locus_tag="OO332_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5940861.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5115..6272)
                     /locus_tag="OO332_000249"
     CDS             complement(5115..6272)
                     /locus_tag="OO332_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5940862.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6572..6796)
                     /locus_tag="OO332_000250"
     CDS             complement(6572..6796)
                     /locus_tag="OO332_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940863.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6938..7099)
                     /locus_tag="OO332_000251"
     CDS             complement(6938..7099)
                     /locus_tag="OO332_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5940864.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7109..7414)
                     /locus_tag="OO332_000252"
     CDS             complement(7109..7414)
                     /locus_tag="OO332_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940865.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7411..7689)
                     /locus_tag="OO332_000253"
     CDS             complement(7411..7689)
                     /locus_tag="OO332_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940866.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7969..9717)
                     /locus_tag="OO332_000254"
     CDS             complement(7969..9717)
                     /locus_tag="OO332_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5940867.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9714..10013)
                     /locus_tag="OO332_000255"
     CDS             complement(9714..10013)
                     /locus_tag="OO332_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5940868.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10019..10252)
                     /locus_tag="OO332_000256"
     CDS             complement(10019..10252)
                     /locus_tag="OO332_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940869.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10254..10474)
                     /locus_tag="OO332_000257"
                     /pseudo
     CDS             complement(10254..10474)
                     /locus_tag="OO332_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10467..10688)
                     /locus_tag="OO332_000258"
     CDS             complement(10467..10688)
                     /locus_tag="OO332_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940870.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10678..10878)
                     /locus_tag="OO332_000259"
     CDS             complement(10678..10878)
                     /locus_tag="OO332_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5940871.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10887..11633)
                     /locus_tag="OO332_000260"
     CDS             complement(10887..11633)
                     /locus_tag="OO332_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940872.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11691..11879)
                     /locus_tag="OO332_000261"
     CDS             complement(11691..11879)
                     /locus_tag="OO332_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5940873.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12284..13513)
                     /locus_tag="OO332_000262"
     CDS             complement(12284..13513)
                     /locus_tag="OO332_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5940874.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13762..14883
                     /gene="roxA"
                     /locus_tag="OO332_000263"
                     /gene_synonym="ycfD"
     CDS             13762..14883
                     /gene="roxA"
                     /locus_tag="OO332_000263"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5940875.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14932..16158)
                     /gene="pepT"
                     /locus_tag="OO332_000264"
     CDS             complement(14932..16158)
                     /gene="pepT"
                     /locus_tag="OO332_000264"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5940876.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16408..17544
                     /gene="potA"
                     /locus_tag="OO332_000265"
     CDS             16408..17544
                     /gene="potA"
                     /locus_tag="OO332_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5940877.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17528..18385
                     /gene="potB"
                     /locus_tag="OO332_000266"
     CDS             17528..18385
                     /gene="potB"
                     /locus_tag="OO332_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5940878.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18382..19176
                     /gene="potC"
                     /locus_tag="OO332_000267"
     CDS             18382..19176
                     /gene="potC"
                     /locus_tag="OO332_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5940879.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19173..20219
                     /gene="potD"
                     /locus_tag="OO332_000268"
     CDS             19173..20219
                     /gene="potD"
                     /locus_tag="OO332_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5940880.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20375..21196)
                     /gene="cobB"
                     /locus_tag="OO332_000269"
     CDS             complement(20375..21196)
                     /gene="cobB"
                     /locus_tag="OO332_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5940881.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21212..22123)
                     /gene="nagK"
                     /locus_tag="OO332_000270"
     CDS             complement(21212..22123)
                     /gene="nagK"
                     /locus_tag="OO332_000270"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5940882.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22152..23396)
                     /gene="lolE"
                     /locus_tag="OO332_000271"
     CDS             complement(22152..23396)
                     /gene="lolE"
                     /locus_tag="OO332_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5940883.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23396..24097)
                     /gene="lolD"
                     /locus_tag="OO332_000272"
     CDS             complement(23396..24097)
                     /gene="lolD"
                     /locus_tag="OO332_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5940884.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24090..25289)
                     /gene="lolC"
                     /locus_tag="OO332_000273"
     CDS             complement(24090..25289)
                     /gene="lolC"
                     /locus_tag="OO332_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5940885.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25551..26597
                     /locus_tag="OO332_000274"
     CDS             25551..26597
                     /locus_tag="OO332_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5940886.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26725..30171
                     /gene="mfd"
                     /locus_tag="OO332_000275"
     CDS             26725..30171
                     /gene="mfd"
                     /locus_tag="OO332_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5940887.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30314..31276
                     /gene="ldtC"
                     /locus_tag="OO332_000276"
                     /pseudo
     CDS             30314..31276
                     /gene="ldtC"
                     /locus_tag="OO332_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31359..31616)
                     /gene="bhsA"
                     /locus_tag="OO332_000277"
     CDS             complement(31359..31616)
                     /gene="bhsA"
                     /locus_tag="OO332_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5940888.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31857..32489
                     /gene="comR"
                     /locus_tag="OO332_000278"
     CDS             31857..32489
                     /gene="comR"
                     /locus_tag="OO332_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5940889.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32551..33090)
                     /locus_tag="OO332_000279"
     CDS             complement(32551..33090)
                     /locus_tag="OO332_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5940890.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33317..34621)
                     /gene="ndh"
                     /locus_tag="OO332_000280"
     CDS             complement(33317..34621)
                     /gene="ndh"
                     /locus_tag="OO332_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5940891.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35022..35564)
                     /locus_tag="OO332_000281"
     CDS             complement(35022..35564)
                     /locus_tag="OO332_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5940892.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35587..36612)
                     /gene="nagZ"
                     /locus_tag="OO332_000282"
     CDS             complement(35587..36612)
                     /gene="nagZ"
                     /locus_tag="OO332_000282"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5940893.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36623..37447)
                     /gene="thiK"
                     /locus_tag="OO332_000283"
     CDS             complement(36623..37447)
                     /gene="thiK"
                     /locus_tag="OO332_000283"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5940894.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37428..38069)
                     /gene="lpoB"
                     /locus_tag="OO332_000284"
     CDS             complement(37428..38069)
                     /gene="lpoB"
                     /locus_tag="OO332_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5940895.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38084..38461)
                     /locus_tag="OO332_000285"
     CDS             complement(38084..38461)
                     /locus_tag="OO332_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5940896.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38464..38823)
                     /gene="hinT"
                     /locus_tag="OO332_000286"
     CDS             complement(38464..38823)
                     /gene="hinT"
                     /locus_tag="OO332_000286"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5940897.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39157..39981
                     /locus_tag="OO332_000287"
     CDS             39157..39981
                     /locus_tag="OO332_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5940898.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39985..41346
                     /gene="fhuE"
                     /locus_tag="OO332_000288"
                     /pseudo
     CDS             39985..41346
                     /gene="fhuE"
                     /locus_tag="OO332_000288"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41406..42839)
                     /gene="ptsG"
                     /locus_tag="OO332_000289"
     CDS             complement(41406..42839)
                     /gene="ptsG"
                     /locus_tag="OO332_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5940899.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43134..43931)
                     /locus_tag="OO332_000290"
     CDS             complement(43134..43931)
                     /locus_tag="OO332_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5940900.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43942..44946)
                     /gene="holB"
                     /locus_tag="OO332_000291"
     CDS             complement(43942..44946)
                     /gene="holB"
                     /locus_tag="OO332_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5940901.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44943..45584)
                     /locus_tag="OO332_000292"
     CDS             complement(44943..45584)
                     /locus_tag="OO332_000292"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5940902.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45574..46596)
                     /gene="yceG"
                     /locus_tag="OO332_000293"
     CDS             complement(45574..46596)
                     /gene="yceG"
                     /locus_tag="OO332_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5940903.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46599..47408)
                     /gene="pabC"
                     /locus_tag="OO332_000294"
     CDS             complement(46599..47408)
                     /gene="pabC"
                     /locus_tag="OO332_000294"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5940904.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47528..48769)
                     /gene="fabF"
                     /locus_tag="OO332_000295"
     CDS             complement(47528..48769)
                     /gene="fabF"
                     /locus_tag="OO332_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5940905.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48856..49092)
                     /gene="acpP"
                     /locus_tag="OO332_000296"
     CDS             complement(48856..49092)
                     /gene="acpP"
                     /locus_tag="OO332_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5940906.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49303..50037)
                     /gene="fabG"
                     /locus_tag="OO332_000297"
     CDS             complement(49303..50037)
                     /gene="fabG"
                     /locus_tag="OO332_000297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5940907.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50050..50979)
                     /gene="fabD"
                     /locus_tag="OO332_000298"
     CDS             complement(50050..50979)
                     /gene="fabD"
                     /locus_tag="OO332_000298"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5940908.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50995..51948)
                     /gene="fabH"
                     /locus_tag="OO332_000299"
     CDS             complement(50995..51948)
                     /gene="fabH"
                     /locus_tag="OO332_000299"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5940909.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(52016..53086)
                     /gene="plsX"
                     /locus_tag="OO332_000300"
     CDS             complement(52016..53086)
                     /gene="plsX"
                     /locus_tag="OO332_000300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5940910.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53167..53340)
                     /gene="rpmF"
                     /locus_tag="OO332_000301"
     CDS             complement(53167..53340)
                     /gene="rpmF"
                     /locus_tag="OO332_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5940911.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53392..53913)
                     /gene="yceD"
                     /locus_tag="OO332_000302"
     CDS             complement(53392..53913)
                     /gene="yceD"
                     /locus_tag="OO332_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5940912.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54112..54696
                     /gene="yceF"
                     /locus_tag="OO332_000303"
     CDS             54112..54696
                     /gene="yceF"
                     /locus_tag="OO332_000303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5940913.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54808..55767)
                     /gene="rluC"
                     /locus_tag="OO332_000304"
     CDS             complement(54808..55767)
                     /gene="rluC"
                     /locus_tag="OO332_000304"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5940914.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56340..59525
                     /gene="rne"
                     /locus_tag="OO332_000305"
     CDS             56340..59525
                     /gene="rne"
                     /locus_tag="OO332_000305"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5940915.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59722..60675)
                     /gene="flgL"
                     /locus_tag="OO332_000306"
     CDS             complement(59722..60675)
                     /gene="flgL"
                     /locus_tag="OO332_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5940916.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60687..62329)
                     /gene="flgK"
                     /locus_tag="OO332_000307"
                     /pseudo
     CDS             complement(60687..62329)
                     /gene="flgK"
                     /locus_tag="OO332_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62395..63336)
                     /gene="flgJ"
                     /locus_tag="OO332_000308"
     CDS             complement(62395..63336)
                     /gene="flgJ"
                     /locus_tag="OO332_000308"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5940917.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63336..64433)
                     /gene="flgI"
                     /locus_tag="OO332_000309"
     CDS             complement(63336..64433)
                     /gene="flgI"
                     /locus_tag="OO332_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5940918.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64445..65143)
                     /gene="flgH"
                     /locus_tag="OO332_000310"
     CDS             complement(64445..65143)
                     /gene="flgH"
                     /locus_tag="OO332_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5940919.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65196..65978)
                     /gene="flgG"
                     /locus_tag="OO332_000311"
     CDS             complement(65196..65978)
                     /gene="flgG"
                     /locus_tag="OO332_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5940920.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66116..66867)
                     /gene="flgF"
                     /locus_tag="OO332_000312"
                     /pseudo
     CDS             complement(66116..66867)
                     /gene="flgF"
                     /locus_tag="OO332_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66887..68086)
                     /gene="flgE"
                     /locus_tag="OO332_000313"
     CDS             complement(66887..68086)
                     /gene="flgE"
                     /locus_tag="OO332_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5940921.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTCAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIVATT
                     QNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQ
                     SSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQIL
                     NTRVNLR"
     gene            complement(68111..68806)
                     /gene="flgD"
                     /locus_tag="OO332_000314"
                     /pseudo
     CDS             complement(68111..68806)
                     /gene="flgD"
                     /locus_tag="OO332_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68818..69221)
                     /gene="flgC"
                     /locus_tag="OO332_000315"
                     /pseudo
     CDS             complement(68818..69221)
                     /gene="flgC"
                     /locus_tag="OO332_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69225..69398)
                     /locus_tag="OO332_000316"
     CDS             complement(69225..69398)
                     /locus_tag="OO332_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCR5940922.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69553..70212
                     /gene="flgA"
                     /locus_tag="OO332_000317"
     CDS             69553..70212
                     /gene="flgA"
                     /locus_tag="OO332_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5940923.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70288..70564
                     /gene="flgM"
                     /locus_tag="OO332_000318"
                     /pseudo
     CDS             70288..70564
                     /gene="flgM"
                     /locus_tag="OO332_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70569..70985
                     /gene="flgN"
                     /locus_tag="OO332_000319"
     CDS             70569..70985
                     /gene="flgN"
                     /locus_tag="OO332_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5940924.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71025..72560)
                     /gene="murJ"
                     /locus_tag="OO332_000320"
     CDS             complement(71025..72560)
                     /gene="murJ"
                     /locus_tag="OO332_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5940925.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72727..73593)
                     /locus_tag="OO332_000321"
     CDS             complement(72727..73593)
                     /locus_tag="OO332_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5940926.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73595..74242)
                     /locus_tag="OO332_000322"
     CDS             complement(73595..74242)
                     /locus_tag="OO332_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5940927.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74253..74837)
                     /gene="rimJ"
                     /locus_tag="OO332_000323"
     CDS             complement(74253..74837)
                     /gene="rimJ"
                     /locus_tag="OO332_000323"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5940928.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75073..76281
                     /gene="mdtH"
                     /locus_tag="OO332_000324"
     CDS             75073..76281
                     /gene="mdtH"
                     /locus_tag="OO332_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5940929.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLICPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76345..76992
                     /gene="grxB"
                     /locus_tag="OO332_000325"
     CDS             76345..76992
                     /gene="grxB"
                     /locus_tag="OO332_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5940930.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77126..77686
                     /locus_tag="OO332_000326"
     CDS             77126..77686
                     /locus_tag="OO332_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5940931.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77792..78838
                     /gene="pyrC"
                     /locus_tag="OO332_000327"
     CDS             77792..78838
                     /gene="pyrC"
                     /locus_tag="OO332_000327"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5940932.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78912..79157
                     /gene="dinI"
                     /locus_tag="OO332_000328"
     CDS             78912..79157
                     /gene="dinI"
                     /locus_tag="OO332_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5940933.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            79447..79701
                     /gene="bssS"
                     /locus_tag="OO332_000329"
     CDS             79447..79701
                     /gene="bssS"
                     /locus_tag="OO332_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5940934.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79816..80934
                     /gene="solA"
                     /locus_tag="OO332_000330"
     CDS             79816..80934
                     /gene="solA"
                     /locus_tag="OO332_000330"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5940935.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80982..81095
                     /locus_tag="OO332_000331"
     CDS             80982..81095
                     /locus_tag="OO332_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5940936.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81356..81922
                     /locus_tag="OO332_000332"
     CDS             81356..81922
                     /locus_tag="OO332_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5940937.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81926..82501
                     /locus_tag="OO332_000333"
     CDS             81926..82501
                     /locus_tag="OO332_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5940938.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82543..83595)
                     /locus_tag="OO332_000334"
     CDS             complement(82543..83595)
                     /locus_tag="OO332_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5940939.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83820..84740
                     /gene="lpxL"
                     /locus_tag="OO332_000335"
     CDS             83820..84740
                     /gene="lpxL"
                     /locus_tag="OO332_000335"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5940940.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84912..85109
                     /locus_tag="OO332_000336"
     CDS             84912..85109
                     /locus_tag="OO332_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5940941.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            85133..>86016
                     /locus_tag="OO332_000337"
     CDS             85133..>86016
                     /locus_tag="OO332_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5940942.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLS"
BASE COUNT        20629 a        22880 c        21180 g        21327 t
ORIGIN      
        1 ctctgcctgg aatgtaccaa acgccatcag cgccgccacg ctcgggtcta tcttgttcgg
       61 tgattttttc ttgttcggct tgatattggc gttcgcgtca ctctgcatca cgacgttact
      121 catcgaccag gacaacaccg gatcgccacg atgcacaatc accctgcggt taacaaaaac
      181 ttcgaacgat ttcgccgccg gactgaatct gaggtaggtt tgcgggaacg gctccacctc
      241 aaaaccagcc ccctgtaatt gcgtcctgag atgcgtggcg ttccacgtat caaagcccac
      301 cagcctgata ttaaatttct ccgcatcctg catgatgtca tctctgatcc ggtcgtaatc
      361 aatgcagtca cctggcgttg tgcgtatcca gcccgcttta gcccactggc gatagacagc
      421 gcggttttta ttggcggggt tctgtagctg gaactccggc agataatgac gggaaatcag
      481 cataatggtt ttaccgaccg gaaaggcata gcatacgctg gaaatatcgc tggttgatga
      541 taagtccagc cccgcgtaac actcctgacc gtgtaaatct tcctccgtga acgttccggc
      601 acactcagcc catgcaccat tacccatcca cggggtagtc ccctgacacc agatattaaa
      661 tcgctttgtc atcatctcca cccactgcga cggaataccc cgcgctttct ggatggttga
      721 ggccagtttt tcacgatcca cggaaacatc aatattaggg ttcgccttta tccacatcgc
      781 cggatcatca acctcgtttt tgtcgtccag ttcgtaaatc agtacaaaaa ttgaatcgtt
      841 gacctcttcg ccgtccagga tctggcagca ataatcatag tgctgtttac aggctgaaac
      901 gacgttactg cccgatgtgg taatggcaaa taacagccct tcgggacgtg cgcccatccc
      961 cagctcaagc gcggaataaa cgccgttatc ggggtgtagg tggtattcgt ccacgatgga
     1021 aagactgggg tttgtcccct cgatggttgc cgcttttgct gccagcggct ttaacaggct
     1081 gttgcttttc gggtgtatca ccttatgcgc ctgaatattc acccgcctgc gtaacggtcg
     1141 ggataaaagg cacatctgac gcgcatcatc aaacacgatc cgcgcctggt cacggctcac
     1201 cgctgcggtg tagatatcct gctgaccgtt ctccataatc agaaaccagt tagccagaat
     1261 cgcggcggtc gtggatttcg catttttgcg cggcacttcg ataaaggcgc tcgtgtattt
     1321 gcgccgtccg gtggccttaa ccttaaagcc gaggatgcac gcaaaggcga actgctgcca
     1381 cggctccagc tcaatgggtc tgccacgcat cggtcctttt acgtgcgggc acaccctgga
     1441 aaaggcaata aaccgctcca caacctcacg atcgaacatg taaagggggc ttttaaggtc
     1501 cgaaaagtac cgtttaacag cctgttttaa ccgtttacag gccggaattt tgcccgtttt
     1561 tacgtcttct gcgtacttat tccaggcggt caagctcgtc ctcttcttct gtttccggtg
     1621 gatttttacg gcggcttatc gggtcaaaac cgagcaagga ggccattttt atcatcactc
     1681 tttcagcgtc ggattttgcg cttaatgcgg ggtttctgct ctcgccgccc tgactgttaa
     1741 caatgctgaa cccgcgcgcc gcaaggtctg caacggcttt ccggtaaatg gagtagttga
     1801 cgcaatacag ttccagattg ctccagtcgg cgggggtcag gtctccccgt tccgcaagct
     1861 gccgcgattt ttcccgccac tgcttcacgg cgatatcatc caggtaggcg ggggctttcg
     1921 gtggtcttgc catgcttatt tttcgtcaga ttatttttca aaaaattccc gtgcataaaa
     1981 atttggggag gcgttcggtg cccggcgggg tcgggtttgt cctgaaaacg ccccccaccc
     2041 cgtcatacag cctcatcagc gattgcggaa acattccata acctcgcggt cacggtcggt
     2101 taatcgcttc gctgtgatgc gttctgcgcg tcctgacgct ttatctttat gccctgtttc
     2161 ctgtgtcttc catgcgtcac gctgccttat aagtccacgg ataaggcggt tttgttcccg
     2221 ctcattcatc agcgtcatac atccagttat tgcggttagc agcccgttct tcctcttcac
     2281 gaaatccacc tgcggcacgc ttgctttttg ttgccggatc aagccatttc gttttctggt
     2341 tatgacacgc ctggcacaat ggttgatggt tccattcggg ccagaagaga acatcatcac
     2401 cgccattaat cgggataatg tgatccacca ccacggcggg cgtatatatc cccttctcaa
     2461 ggcatcgcac gcataacggg tttttactca gatacatggc gcggtatttg tcccactgtc
     2521 tggaatatcc acgcgcgcgg cggtgtcctc gtctggcatc ctctgcacgc catgccgccc
     2581 gcctgtgctc ctcacacttg ccggatttaa cgcgcctgtt acagcctggt tctgtgcatc
     2641 gtcttaatgg ttgccacggc atcagtacac ccccacatca cggtaagccg tccagagtgc
     2701 gcctatcgtc atgggtacgc gtgtttttgc gttatccgcg acaatctggc gattttcata
     2761 caggtgagcg ataaacatca tgcagccaat ctttatggct ggcgtgaacg ccagcccgtc
     2821 atcaaagcgc ctgcctatgt gtatctggca cgcctcaagc gatgcggcaa tgtatccgct
     2881 gattaactgg tcttcatcgt cgccatcgat gcggcaatgg agtttcactt cttccagggt
     2941 gataagttct tctgtcattt ttccgcgccc tcacgacaaa gaatttcaag gcgtgtcctg
     3001 gcggcatccg gcagcggctg cccgatgata ttcagtacac gccccgccag cggccccgta
     3061 ctgaccttta tccggctggt ggcgttgatg tccttccggt accgtatcca gatccttacg
     3121 gtaccaatcg ccagctctgc gcccgatgaa atggcctcct tgctgctgac catgttcacg
     3181 cttgcccaga gtgtgtgacc gtcctcccac gtttcgagta tttcgccggt tatatccctg
     3241 gtctgtttca gggtctgaat cgttatcctg tcacgcagtc gccctatgtt cattccggtt
     3301 tttctccctc gctgattttt acttcctgtt tccatgcctg gctgaactca tcaccaccat
     3361 cacgcggtga cagcccttca cgttcgcggg cttcgttcgg acacataacg ccggatttta
     3421 tgccgcgctc ataggtggca aagcgttcgc caggtgtggc ccgtaacagg tccgcgctgt
     3481 caaactccac ctgataccgg ataccaggta caggcgaggc caccagcaag gcatttttta
     3541 tctgctgctc aaagttcgcc agccacgggc gcatcgtcat ggtgagaaat gcgcggcttg
     3601 cctcgctgaa gttgctgtag gtgctgttgc tgtattcctg caaaaaaatc ggcgagacgt
     3661 tgaacattcg ggcgatgtct tcaatggtga agcgacggga ggccagccat tcggcatcct
     3721 gattactcat gcccagctgc tgatagctca tccccccttc aaggatgggc gtttttccgg
     3781 cgtttctggc ccctttgtag cgttccagtg cggctaatgc ctgtttgccc ttcacgccgt
     3841 ccagccattc gcctgacgtg ataacccctg ccgccatcat gccatcacgc atcacgctcg
     3901 cgccgtggcg ttgttgggcc agcccaagcc ccagcgattc gcggcagatg gttacaggtg
     3961 agcgccccat aaaaccgtca tccgtggagt aacgaaggtg gagaacctcc cacggtaaat
     4021 agttgcgggt gtttccggtg taggcgtcag tgatgcagta gcgccagtta tgatcacctg
     4081 tctgctccac gttcaccgac tgcggcgggt aaggatgtaa agccgccgga aaaccatcac
     4141 gcccccactg aataaccgca taagcattac cacttaacag gcagtggcgg atcatcattc
     4201 gcttaaactg gtagggtgtc tgccacgcgt tcgggcgctc gttgaggatg tgatcgaccg
     4261 gatgagaatc aagccattcg cgggcctcct tccccttctc attgcgtacc aggtacaggt
     4321 aacacggcat ggtagccacc gcctcagaga tgaccgtgac ggcgttcatg acggcggaga
     4381 gtgattccgc tgtcccagat gatacgtact cacccgcccc cgtgttcgat gtgccggcca
     4441 gcgccataaa ttcatcaagc gtcatgctgc gctgctcttt ttttcgtctg aaaggccaca
     4501 tatcacaccc ccgctaaatc cgcccaccag cggcgattat cctcacgcgg cattttttcg
     4561 gggtgctgct catacaggga acggcgggcc agctccacgc cggaatcggg gtaagccggt
     4621 accgatgtaa cggtaatttc gtacagttcc gccaccagca cggtgcgcac gcatggatct
     4681 gttgtggtat cccatacatc cttacgggag cgaaagctaa agctcatccc cgagatgtcc
     4741 ccacgcttaa ccagttcgat aacgtcgcgc cctgtactgg tatccggtgg ggtcagttca
     4801 aagcgtaacc ctgtttcgtc ctcttccagt ttcagcgtgc cggaacgggt gcgccccagt
     4861 aacatgctgt ggtcatgctc atacaggccg cgaacgtcat tacccgccgc aagccattca
     4921 gtaaacgccc cccgctggaa tttttcgtaa aactcacccc ataacagttc tgaaagctta
     4981 tcccagcgaa caacgtagcc cgtcagcgta ccggctccgc tggtggtgat ttccgatgac
     5041 cggatttcca tactcttcat aattttttca cccataaaac actaaagggg ctttttagcc
     5101 ccttctgtat gctgttaatc gtcgtcttcc ggcagttcca gaatcttgat cgcgttcgaa
     5161 tccaccacgc caccgcctaa atatttttgc gtgaaaattt tgatgaatcc tggttctgtc
     5221 aggttgtccg gtctggtgcg aacacctgtt tcgtgatcaa caatgtagta accgcgtttg
     5281 aagtcaccca gggcaataac gttatcaggc ataaactcca gatattcgac cggaaggccc
     5341 agcaacgtat caggatcacc cgcctgtaaa cggtcgcgcc agatataatc accgttcgcg
     5401 ttcttcacct tctgaagttt tgccgccgtc gtggagttaa ccacccagac cgcgtttttg
     5461 cggtattttt tacgtaatgc aaatttcagg tcgatcagcg ggtccgcaga tgtccacgcc
     5521 agagattcgg aaggtttaat tacctgcaac gtaccaaaat cacgctcttt gtcgttcttc
     5581 tctgcacggg gtacggataa aaaacctttt gcttttttgt caccgtcgcc cacaaccaga
     5641 tcgctttctt cggtttccgt gaaggtgtcg ccaatctcac ccgtcagcca tgaaaggatg
     5701 tccacatcgg aaaaatccac gatttcctgt gtggtgcgcg ggtacgcata gaccggatac
     5761 agcttaatgc tcacctcgtt aatctgcggg gtgctggtct gttcgcgtgc cttaccctct
     5821 tcaccgtggt taacggtcgc accgccagcg gaaacaagct gcttaaactc gttgctgctg
     5881 attttcttca cggtacagat gcggcgcata gtggattcat ccgccagcat tcgcatgatt
     5941 tcggtgttca gttcagggat aacggtataa ccaccatcag cgggaacgcc tgtacttaat
     6001 gcgcgggttt caccggtcag aatgtagttg cgtagttctg cggggtcagt ggtctggctg
     6061 cttttacctg gcttgctgcg ctcttcgtct gcaatggctt caaggcggga aatgtcttta
     6121 tcgagggatt cagctttagc gcgtaattcg tcaaattttg cgccctcagc atcgttaaga
     6181 ctgcggtttt ctttttcagc gttctccagc atgtcgcgca tctgattttt aatggcggtt
     6241 ttctgctggc gtaattcgat tattctctgc ataaaaaagt cctggggtta agtaaggaac
     6301 tccaggacgc ggcaaacact caaccgtttt tcataaggaa atcagtaatc gcaccgatcg
     6361 ttttcccgcc tggtaatgaa tattggcgag cacattaaca ggcgggaaag tggccccagc
     6421 gtcctggcac cacgggcgag aataatcatg attcagttcg ggtaaaatat gccgatccgc
     6481 tcagtgaaca acgtggaaca accacgaaca aataatttac aaaaaataac aaaaagccgg
     6541 attgctccgg ctgtatgtgc tgtatgggtg ctcagtctct gatccctttc agaaattcta
     6601 tcaatgcatc tgtctgttca ggatttactg ccagcatttc accggatagc gcgcaacgaa
     6661 caaaaccatg atgatccttt tcaatcagcg cccccgtttc cagaaatgcg cggtaatcag
     6721 tgatattcat cgtctccatg ttgtcagcat ggtatttatc acgctgttta agtatctcat
     6781 caaatttcat cggcattgcg ttttcctctg ttgtgtctgt ttgtttcaga tagtaactat
     6841 gccggaccgt gacgaaaagc cagtagtgct ccataccgtt tcaaatgacg caaaaaaagc
     6901 cggattgctc cggcttcgtg gtgtggttct gatattccta ctctgctaat ttgctcagcc
     6961 ccatgtctgc gtaagtgcga tttactgcat ttctcaggtc tgcgtagttc tccgttggcg
     7021 gctccggtgt gcccttgctg ctctgtttta ccagcgggtc gcccaccagc cgcccgtaag
     7081 ctgcaatttg tgctgtcata ttgccacctc agtgaagcga tccggcattg ttctttttca
     7141 tctcttccat cattttttca gtgagcatta cgcactgaaa ccccatgttt ttgatgtcta
     7201 cgggtttcat gcctttgtca gtggatatca caggcagaat atcctgatac acatcgcggt
     7261 cagggtaact aagcacacaa agcgcccatg atgccacggg tgaggatcct tcgtctgttt
     7321 caacccacca gcctggtgta gctgcgctta tttgcagaat cgtgatccgt ccgttgtgct
     7381 tgtaaaattt ttcaccaaat tttggttcgc tcatgattcc acctctccgg ttttaacgtt
     7441 gattgttgtt acctgttccg cttcggcaat ctcccgctct gtcagcgtgg caaaatttgc
     7501 cgccgtcgtg gtcatgaatg cgcttatcag ttcgggatgt gctttcgcgt atccttcccc
     7561 cgcgtggcgg tctatcgttc tgattgccac ctttaaagcg tgctctgtca tgtctaacgc
     7621 gcgatatttc ggttctgttc tgtctctgcg ttttttgagt gatttattaa atttccctga
     7681 agtgtgcata tttattttta ccccctcgtt taaaaagttt tgagttgtgc ctccccttgt
     7741 ctaccttagt ggccctcatg ccagtaatgg cgctgctttc agcggggtag agtgctttta
     7801 tccactatct accccgtgtc tacctccctg tctgattcag gtaaaatcag gtagagaggg
     7861 tagatagtgg gtagacagta aaaaaaggct acctacctaa cttaatgcac tgaattaaat
     7921 gtatttttct ttactcaggt agatagggta gacagcaatt acaaaaaatt ataaaaacgc
     7981 gtcgcactcg tctgttgtta ttgcgttagt ctgcgttact cccttaactt tccgcgtaat
     8041 atattcatgt ccgtaaactt tcgcggctgg cttcatagcc ttgccaaagt catttacgtt
     8101 tagcggtttg ctcctgcctg cgtacgccat aaacgccaga tagacgcggt aaaggctgtt
     8161 tctggtcgtg tacttcactg aatcgccacc gccgcccatc atcaggccgc gtgcttcctc
     8221 cagaaaattc aggaactggc aaaactcaat aaccggatcc gtctgttgct ttattgccag
     8281 agcttcatca ccgtcacgct gttccagtag taaagcccgt gctttctcag agtcggtaaa
     8341 gttcgccagc aatcggcgga taatgacagg gatttcagcc gcaatctttt ccggtagctc
     8401 cctgtctttt tcggcctcac taacgatatt gtcgaaacgg aaaatcacgc gacgacgtgc
     8461 cacacctccg gcccgttcgg tgaatatcat cgggttattg ttggtcgcca gcaccaccgc
     8521 cctgattaca gccgtgaaac gcttttcgta tttcgggtta atttccacgg ggtcaccgcc
     8581 cgtgattttc ttgatgcccg ttccttcgcc tgtatatttc ggctggtcag ccaggacgat
     8641 aagacgactc ccgacaacct gcggacgtcc accagcatca tcaagcgatg tcatttcagc
     8701 gcttaccgtg ttctgtttcc ctgccagaag gctggctatg tgtgtgaatg tacttttacc
     8761 gctcccgccg tctccggtgg cctcaataaa catctgccag tcgtaccggt tcgccataat
     8821 catgtacagc gcggcacata tacgcatcat cttgcgctgg tcttttccgg ctgcgtgctc
     8881 aagccattta tgaaagttta gcgcgttatc gcggatgttc tcccctggtg ctggtggcgt
     8941 gtactcaatg ccgttgtgcg tggtgatcca gttctccggc gtgtgcgggg aaaattcccc
     9001 cgttttcagg tcaagcgcac cattggcgaa cggcagcaaa tcgccggacg gctcgcccat
     9061 tggttcggca ataactttta acgcttccac ggcgttattg attacgcgct tgctgaaagt
     9121 ggccctgtgc tctgaataga tcgccaccat ttcgcggcta agttccattg tgctgaccgg
     9181 acaccatacc ccgccgcgcc atacgtgaac gatttcactt tcctgatgca cacaaacgcc
     9241 atcaaagcgc tcggcaagca gctgcgcgcg ctcactgtct gccatttgtg ccagttgcgt
     9301 atttttctgc tccggcatgg taagccctgc ggcaatattt tcacgctcag cattcagata
     9361 gcgccgccag ttttcacact tctggacgtg cattccttcg gggtaaaaat ttgcgtcctg
     9421 aatatctgcc gccgccagct tctgaccaat ctttttggtc tccaccaaat ccagttctcc
     9481 ggcctggtac agccttacgc gtttttttcc atccggaaca attttcagtg catcaagttc
     9541 ggcaagctga tttggcccaa gccacacagg cggcacatta tcgccggatg cggggccatc
     9601 ctgctcctgc cattgtttcg cgtgcgacca ggcatcacta cccgcaaaga taattacctc
     9661 tgtgtctttg tgttttattc cgcgtggctg tttttttacg ttcggtgcca gtttcatttt
     9721 ttacccctga atacgttaag catctttttt atttcctgaa tattggcgcg tgctttctcc
     9781 ctgctggttg gtttactgcg gggcgctgcc tgtaccagcg tgaaatcacg ccggaattga
     9841 taaacaggca tcacgcaatc atattcgtaa ccttcacggc ggtaggtgat gcgccgttct
     9901 gccacgcctt taatcgttac catgccgccg tactggtcgc ggtaaatatc accgcgcgta
     9961 aatttagggt gagtgttgcc actggcagtt aagccagaat atttaagttt cattattttt
    10021 attctccggt gtgctgttct ttatatctgt cgtgcaatag atctatttct tgcagttcca
    10081 ttattacagg ctcaagaagc gttattaatg ccgtggcaat tcttgatttt tgtttgtcgc
    10141 gttcattgtc gccaagtgtt tcaagccata tgcgcaatat ttccagcatg ttttcactgt
    10201 gagaaagtgc aagaaatgcg cggtctattg tttcgtggta aatatcacgc atattaatcc
    10261 ccgtccgtcg tttttcttaa aataacctct gtcacgaaat cagcataatc ggcggcgata
    10321 ttcagtacat caagcccgtt gatttatatt ctctcgtgga aagtaagaaa aaagccgctc
    10381 taataagttc tggcattgac gaaagcgcat cagccgcatc atcaggaacg ccggaaaatt
    10441 cctgtttcag ggaattaaaa cgatcatcac gcataacccc ccccattttc acaatcggca
    10501 acaagaatat ttttagcgtc attcagtgac cgcgttgcga tgtatcggat acattccagg
    10561 gcgaactgtg tccggtcttt ctgttcttct ccctgcgcaa gctctgccgt gcattcaata
    10621 tcaataagcg cgtgcatcag cgtagtgagt gcggcggctg ctgcgttcgg tgtggtttta
    10681 ttgtgcatag gtttccccct gacgaatacg ggcggcgaat acagcaacac aaccggacgg
    10741 gcaacggcta cgcgcttcgc gttccgtcca agcggttacg tggatgattt gggattctcc
    10801 ggtactcagt gccagaaaac gccacacaaa ggccgtttgt gtgtgtacaa ggtgtggtat
    10861 atgatttacg gcaaccataa cggctcctag tttacgttgt tggttagaag ccccgttact
    10921 gctcctacag tgcggggctt tgctttttgt gttgattgtg tgtaatgtgt cattacacat
    10981 aaatacatta catgaggtgt aattaacgtg tcaacacaca aaaacgaaag gcgtggtaat
    11041 cctccgttcc agttccgttt agatcctgac ttacgggaaa agatggaaaa ggcacaacaa
    11101 caggacggcg atgattctct tgctgcctgg ataaagcgaa taatccgcaa agaattacag
    11161 caacgcggca tagaacccaa aggataaaaa ctatcagcgc cgtggtgtga ggtactacgg
    11221 cgcattgctt tacaggcgca cacaatgacc aacgaagaat caaccgaaaa aacatcacct
    11281 gcacgaaaaa gacgacgcag aaagaaagag catgaatcag aacgattcgc gccgtgttct
    11341 tttgcccttg agaagtacct taaagaatac tcatcaaagg agagaacttc gcagttatgg
    11401 caacgcactg aaccagactg attgcattgc ccaccagcct gatagcggct atcatccccg
    11461 tgcttatgtt tgggatcaca cacacaaacg gcgcagcggg ttatctgttc agaaaggcgg
    11521 ctccatttcg gggccgcttt ttttatgcct gaaaaacccc aattttgtgg ttttccagtt
    11581 tcaccagggc gaacgaatcc ccgcccacgt tctggcgtat attcaatctt catggttata
    11641 gctctgtgtt cagatgattg atgtgtggcg gctgcgtgcc gccagcgtga ttaatgaact
    11701 gccttgcagc tatccttcca ggccagaacc tcggataaag accagccaac ggaacgaccg
    11761 ccaagtttac gacgtgatgg gaattgtccg gccttttcca ggcggtagcg gcatgagcgg
    11821 ctaaggcctg ttagcttttc gcattctttt tcacgtataa accgatcagt gcttaacact
    11881 attgccccct ttcgtttctt aaagagttat ttcgtgttct attgcgttgg gatgtgtctg
    11941 attgtgtcag gatgattcag agttggcaaa tgttgaggcc gtatggttta cagaaagagg
    12001 aataatcagg ataaaatcat ttaaattcat gttaatacaa aggcataaaa tattgtttca
    12061 tgcctttttt ctcgctattt aaagagtgat tcgctagtgt ataaaaaacc agtaagacgt
    12121 taaaaatcag ctacttataa atctgtacac tttttcacct cttgttcgta gttgttccgc
    12181 attgttgctc attgttgctc attgttgctc attgttgctc attgttgcac gttgtatctg
    12241 ttcgcatatc cagtatgcgc atactgaaaa aacacgaaaa aaattatttt cttctggcta
    12301 ctggtagcgt ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc tccgtccaca
    12361 tatccagcgc attgcgttta gcatccagat aacgggaatg attataaact cgctgcattc
    12421 ctggcatctg gtggcctgta agctgctcca cgacgtgagg atccacgcct aaatcgttca
    12481 gcatcgttgt aaaggtgcgc cggatgtcat gtagtgacca gtgagggtga tttagcctcc
    12541 tgtgcgctaa tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc cccagcaata
    12601 agcccgtgtg cctgttctgc tccaccagct gcgtgacgaa cggcagtatt gcttccggta
    12661 tgggccggaa tattgccacc ttcgttttgc tgtgttcttt cggcacggtc cagagcattt
    12721 cggtaaaatc ccactcgctg atctccgata acctcagttc taccgtccgg catccgaaca
    12781 caatcaggag gcggattaac gcgatgtagt agggggagaa tatttttttg tccagtgcct
    12841 gcaataattc gcccagttct ttggtgctta agacacgctc gcttatatcc ggttttttcc
    12901 caacgtccgc cacactcata tcatcaagaa cgttgctgat tgcatagcgc cgcctccggc
    12961 agaacttaag cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca ggcgttcgct
    13021 ttgccacctg gtcaaaacag gccagccagt gccgtagctc gcatttatcc agcggcatag
    13081 caccaatgtg ctgtattacg tgattattaa ggcgcttttt cagggcggca taatccacgc
    13141 ggttttcctt tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc gttaccggct
    13201 ttaacgcttc ctgtacggtg taattaagct catgacgtgg atttttcccc tctgccagcc
    13261 atgcgcgaca ctgggcggct ttttccctgg ctgatttcag gctcagatca ggataatttc
    13321 ccagcttaat gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa gtgaaatacc
    13381 aggttaaaag gccactggtt gaatgcctga cgctcaggtt tccaccgtca ttaagaaagg
    13441 ctgttttttg ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc agtttgttaa
    13501 gtgctctgct cataattttg attccggtca tcacaattaa ctacaccatt aactacaccg
    13561 atcgttgcac aaagggctac aacgtgaaac aagctggaac aaggaaaatc acaaatctgt
    13621 ttataatcaa aaacataatg aacaatctga aacattatga aacggcaaaa aacactaatt
    13681 gataaaatat gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat
    13741 accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt
    13801 gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac
    13861 ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg
    13921 gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat
    13981 ctcggtgaaa ccaactggtc attactggta caggcagtaa accactggca tgagccgacc
    14041 gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt
    14101 tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc
    14161 attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac
    14221 tgcccacatc cagatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg
    14281 gagcctggtg atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa
    14341 aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt aattagcgga
    14401 tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga tccggatgtt
    14461 ccacctcgcg ctcatcctgc ggatgttctg ccgcaagaga tggataaact gcgtgagatg
    14521 atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc
    14581 cagtcacgtc atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc
    14641 tacgatgcac tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc
    14701 attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg
    14761 gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat
    14821 ccgtcattcc tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg
    14881 taagtttgga tttaagccgg atgcagcatc acatgccgca tcccttttcg cttacttccg
    14941 ttgcgccgtt aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag
    15001 aatcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaggt tcgggcaagg
    15061 taatcccata aacgacaact gcgcgccgtt ggtaccgccg cggatcggtt tcagttccgg
    15121 ttcaatatcg cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac
    15181 cactttctcg cgcatattgt agtaactgtc ttcaatcacc aattcaatgt agcaatcagg
    15241 atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa
    15301 ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt
    15361 catgctcgcc aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc
    15421 cggaacttcc gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc
    15481 cgtgcccgga tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt
    15541 ttcaaactcc agttcgccta cgccaccacc gtcaacagtg taagcccagc gggcatcgaa
    15601 ggcatcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac
    15661 gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc
    15721 tgcaatacct gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt
    15781 ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat
    15841 acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc
    15901 gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg
    15961 gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag
    16021 ccccatttct tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct
    16081 gggaacctgt ctcacccctg cttttgattg ggtatccaga gacacgtagt tcaaaaatcg
    16141 ctcaagtagt ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa
    16201 gaaaagacaa atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca
    16261 ttaccttaat gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg
    16321 gtttgccgta gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg
    16381 catcggtaag ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg
    16441 agttcgcttt caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag
    16501 gtcattcccc agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct
    16561 tctggctgcg gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc
    16621 ggacgcatca tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg
    16681 aacactgttt tccaaagcta tgcacttttc ccccacatga ccgtgttcga aaatgtggcc
    16741 tttgggttgc gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc
    16801 ctgcggatgg tgcagctgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt
    16861 caacagcaac gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg
    16921 gatgagtcgc tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa
    16981 gcgttacagc gtaagcttgg cattacattc gttttcgtga ctcacgacca ggaagaagca
    17041 ctcaccatgt cggacaggat tgtggtgatg cgcgatggtc gcattgagca agacggcacg
    17101 ccgcgtgaaa tctacgaaga gccgaaaaac ctgtttgttg ccgggttcat tggcgaaatt
    17161 aatatgttta acgccacggt gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt
    17221 gaaggccgcg aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat
    17281 gttctgctgc gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa
    17341 gggctgattg gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt
    17401 gaactggaaa atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgatcctgac
    17461 tttgaccact ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta
    17521 ctggctgatg aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg
    17581 gcttgtgttg tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg
    17641 cgacgacgcc agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga
    17701 tccgctctat tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc
    17761 ctgcctggtg ctgggctatc catttgcctg gtttctggcg aagctgccac acaaggtgcg
    17821 tccgctgctg ctgtttctgc tgattgttcc gttctggacc aactcattga ttcgtatcta
    17881 cgggctgaaa attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg
    17941 cgttatcgac acaccaatcc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt
    18001 ttacattctg ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa
    18061 gccgttgctt gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgcat
    18121 cattattcca ctgacgatgc cggggattat tgccggatgt ctgctggtga tgctgccagc
    18181 gatgggcctg ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa
    18241 cgtcatcaag gtacagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat
    18301 tacgctgact atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct
    18361 gaataagaag gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct
    18421 atctacgcgt acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg
    18481 cgctttggca tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac
    18541 gatagcctgt tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacgttt
    18601 gctacgctta tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgcggtaag
    18661 ccgttcgtta gcggaatgct gtttgtggta atgatgtcac cagatatcgt gatggcaatt
    18721 tcgctgctgg tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc
    18781 tcgcatatca ccttctgcct gccatttgtg gtggtgacgg tgtattcgcg cctgaaaggt
    18841 tttgacgtgc ggatgctgga agcggcgaaa gatctcggtg ccagcgaatt taccattctg
    18901 cgaaaaatca ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt
    18961 accctgtcga tggacgatgt ggtagtttct tcattcgtca ccggaccaag ttatgaaatt
    19021 ctgccgttaa aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg
    19081 gcaaccatat tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt
    19141 gataaaacga aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg
    19201 cggcgggtgc tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt
    19261 atttctacaa ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa
    19321 ccggtattaa ggttatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga
    19381 aaacgtacaa agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa
    19441 tgcgtaaaga ggggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc
    19501 tcgatccaga catgctcaac aagccttttg acccgaataa cgactactcc attccgtata
    19561 tctggggtgc gacggcgatt ggtgttaacg gtgatgcagt ggatccgaaa tctgtcacca
    19621 gctgggccga tctgtggaag ccagagtaca aaggcagcct gctgttgacc gacgatgccc
    19681 gtgaagtgtt ccagatggcg ctgcgtaagc tgggctactc cggtaacacc accgatccga
    19741 aagagattga agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta
    19801 actccgataa cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga
    19861 acggttctgc tttcgttgca cgccaggcgg gtacgcctat tgacgtggtg tggccgaaag
    19921 aaggcggcat attctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag
    19981 gcgcgctgaa attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgctgaaa
    20041 ctatcggtta tccaacgcca aaccttgcgg cgcgtaagct gttaagtcca gaagtggcaa
    20101 acgataagac gctctacccg gatgctgaaa ccattaaaaa tggcgaatgg cagaatgacg
    20161 ttggcgcagc cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat
    20221 tcaacaagcg gacggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga
    20281 gtcaggacag taaagtttta tcccgctctt tcgtcggtat tgtggtccgc ttgtaggcct
    20341 gataagcgta gcgcatcagg caatgcttcc tgctttataa tccctccagc aacttttcaa
    20401 caaattctgg caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccaa
    20461 cctgactcgg ttcaagattc agttcaacgg tgtgagcacc atgtagtttg gcttcgtgta
    20521 caaacccagc agccggatag acatgcccgg aagtaccaat ggcaataaag acatccgcca
    20581 tcgacaacgc catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt
    20641 gcggacgcaa gggtgccggg aagtggcaac aatggcattt atcttctggc gtaacgtctc
    20701 cggtccagtc gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca
    20761 tatgaatgac attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca
    20821 gcaaaaagcg atcgccgaga gcgtcttgca gtttagccag cgcaagatgc gcggcgttag
    20881 gctgaatttc tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt
    20941 caggatcgcg atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca
    21001 ggccatctgc ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca
    21061 gtacgagtac tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac
    21121 gctgacgcaa acgctcgcgc aggcgacgtt tatttttacg aaaacgactt aaccgatgac
    21181 cccgacgcga cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac
    21241 cacgcattcc tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag
    21301 gtaagagatg acaaggcaac ctgtccgcca gttgcgttgt gattgccggg aaattcgata
    21361 agccaccacc aatgacgacc aggtcagggt caacaatggt caggatattt cccagagaaa
    21421 ccgctaataa atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat
    21481 aaagcgcaat aatttcggga gcctgcaacg gttgatgata atagtgttga tacagccacg
    21541 caaaaccgcg accagacaga taattttcaa tgcagccatg ctgaccacag ccgcagcggc
    21601 gtaacgggaa atccaacccc atcatggtta acgcatcaac cggcagacgc atatggccaa
    21661 actcgccggt aatgtagctt ttaccagtaa tcggtttgcc gttgaaaacc agcccgccgc
    21721 caacgccggt gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcctccc
    21781 aggcttctga aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac
    21841 gtgcgctcag gtcggcacgc agcggtttgc cgctggcagc ggggacatta gcggcataca
    21901 gcttaccatc ttctgtttcc ggcattcccg gaataccgat gccgacagaa cctttacagc
    21961 caaaacgttg gtcagcttca gctaccagct cacacactgc atctaaaaat gcgtcatagc
    22021 tgtcacgcgg tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc
    22081 caagcgcaat ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta
    22141 atgtactgcc tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc
    22201 gccggatacc aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg
    22261 tccagccagt gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac
    22321 tgatgaccga tcagcttttc aatccactca ataatcgggg taagttgcag tgaaacaact
    22381 acgccgataa ccaccccaca caggctgccg aacagccctg ccagcaatcc ataccagaca
    22441 aagatggcgc gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca
    22501 ctcttgtctt tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc
    22561 accagtacca tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag
    22621 ccgtaagtac caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg
    22681 cgtaccagct tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg
    22741 gaacccatat caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg
    22801 agttgaccac tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc
    22861 agtttatgct cgggattcga gttggggatc atgatcgaca cccaatcgcc ctgcttcact
    22921 ttcagcgcat ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg
    22981 cgccacgcat ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgtggg
    23041 ttaacgccct tcacctggat tgcgcgcaga ttcgctccac tttccaccag accggtgaaa
    23101 ttgatatacg gcgcagcggc ggcaataccc ggcactttct gcacgttatc cagtgcttcc
    23161 tgccagttag tccatggctg atttaccgct tcgatttcgc catgcggcac caccgccaga
    23221 atgcggttat tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc
    23281 gccacgccga gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg
    23341 cgtcgccgtc cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact
    23401 ccgcccccat caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc
    23461 tcatacgttt cgccagttgc aggtcgtgag tgaccaccag gaaagcggtg ccctgcaagc
    23521 gattcaactc tccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg
    23581 taggttcatc cgccagtacc agacgcggat tattgaccag cgcacgggca atcgctacac
    23641 gctggcgttc gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca
    23701 ccgcttttaa catctccagt gcacggctgt tgatttcagc gggctttttc ttgccaatca
    23761 gcagtggcat agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat
    23821 aaataaagcc cagcttctgg ttgcgcagtt ctgctttcgc cgctgaagac agtttgctca
    23881 tcggctgacc gttaaagatc acatcgccgg aggttggcgt atccagcccg cctaacaggt
    23941 gcagcaaggt acttttaccg gaaccagagc taccgacaat tgccatcatt tcaccttcgc
    24001 ccacgctgaa actgacattg tgcagcacat cggtttgcac actgccttcc tgatagcgtt
    24061 tgcacaggtt gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttgt
    24121 gtggcggcag cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc
    24181 agcgcaataa caatgacctg taaaggttcg atagccaccg gcagcgccgc gccatcaagc
    24241 aggacgccga ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc
    24301 gctccgagga ttgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg
    24361 atttgtcgcg gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatc
    24421 accatcagcc ccagcgaggt aataatgtta aacgccgcaa cagcgacaat caggctcagc
    24481 agtaagccca tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca
    24541 cgccagtcct gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc
    24601 agcggctcat ccagccacaa acgccagccg gtaatattgg ctgccggata acgcatcaga
    24661 cgcgaggcat cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg
    24721 aaagtgccaa tcacattgaa caggcgctga cttggaatac gccccatcgg cgtgaactgg
    24781 ctggcagatg gcaccatcac gcggatttga tcaccgcgat tgacgcccaa ctgtgaggca
    24841 agttgttcgc cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg
    24901 accagatacg gcgttagtgg atctttttgc gccgggtcga taccgagcat caccccgacc
    24961 gccacgctgc gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta
    25021 acgccgtcca gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca
    25081 gaagagagaa ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag
    25141 ccgttcatca ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta
    25201 gaaagccagg agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcaag
    25261 ccaataaata gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac
    25321 gagtatataa gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta
    25381 atctgaaaag cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc
    25441 gcctgatctt aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca
    25501 taaccagcag gatcaaccgc atgtaaatta aaaaagagcg tgttgttctg atgaaacaaa
    25561 aagagctatg gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact
    25621 cggtcattac cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga
    25681 gcaaatgctg gatctatttc aatctttacc ttgccccctt tcgtatgccg gtttttttct
    25741 ttatatccgg ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg
    25801 ataaacggat ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg
    25861 cacctgagcg cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt
    25921 tcctgcacgg gatgatcacc gccagcacca gcttgtggta tctgtatgcg ttaattgtct
    25981 atttcgtggt atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac
    26041 tgctgagtgt ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatac
    26101 gcaatttgcc ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta
    26161 aagaggtgcc gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg
    26221 gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga
    26281 agctgtttta tcagtacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga
    26341 ttggctccaa caccattgct atctacacca cccatcgcat tctggttgaa atattcagct
    26401 taactctgct tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc
    26461 tgctggttta tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa
    26521 aactttcaca gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg
    26581 tcagttactc tcgttaaacc atcaccccag ccggggcgat tcaatttgct aaaccatgtc
    26641 gattacggat aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat
    26701 cctaacgaga atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa
    26761 gcgggtgagc agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg
    26821 gaaattgccg aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct
    26881 ctgcgtttgc atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac
    26941 tgggaaactc ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt
    27001 tccacccttt accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg
    27061 cttatgcagc gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa
    27121 ggtcagcgcc tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgctat
    27181 gttgaccagg tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc
    27241 ccgatgggga gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg
    27301 cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg
    27361 cccgcacacg aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc
    27421 gataccttcg aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca
    27481 ttacctgccg ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg
    27541 ttcagttatt tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc
    27601 gaacgtttcc aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc
    27661 ccgctgttgc caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa
    27721 aactggcccc gagtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat
    27781 ttaggtttcc agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat
    27841 gcgctgcgta agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa
    27901 ggtcgccgtg aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt
    27961 atgcgtcttg atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat
    28021 ggttttgtcg ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa
    28081 cgtgttgccc gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt
    28141 aaccttgcgg agctgcatat tggtcagccg gtggtccatc tggagcacgg cgtcggtcgc
    28201 tatgccggaa tgaccacgct ggaagctggc ggcattactg gcgagtacct gatgctcacc
    28261 tatgccaacg acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac
    28321 gcaggtggcg cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc
    28381 gcgcggcaga aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac
    28441 gcgcaacgcg ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg
    28501 ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta
    28561 cttaacgaca tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt
    28621 ggtaaaacag aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggta
    28681 gcggtgttgg tgcctaccac ccttcttgcg cagcagcatt acgacaactt ccgcgaccgt
    28741 ttcgccaact ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag
    28801 acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa
    28861 ctgctgcaaa gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac
    28921 cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg
    28981 acgcttactg caacgccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac
    29041 ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag
    29101 tatgacagcc tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt
    29161 tattatctct acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg
    29221 gtgcctgaag cacggattgc catcggtcac gggcagatgc gcgagcgcga actggaacgg
    29281 gtgatgaatg atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa
    29341 accgggatcg acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt
    29401 ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt cacatcatca ggcatatgca
    29461 tggctgctga cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg
    29521 attgcctcgc tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt
    29581 cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc
    29641 tcgctgtata tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg
    29701 tcgctggaag atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg
    29761 ccagatgatt tcatccctga cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc
    29821 gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg
    29881 ctgccagatc cggcgcgtac cctgctggat attgcccgtc tgcgccagca agcgcagaaa
    29941 ctggggatca ggaagctgga aggtaatgag aaaggcggga tgatcgaatt tgccgagaag
    30001 aatcacgtta atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc
    30061 cttgatggtc cgacgcgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc
    30121 gaatgggtac gccagtttat gcgtgaactg gaagagaacg cgatcgctta atgtaaatcg
    30181 ggggcgtttc tgcgccccca tctgcaacat ttacaaattc tttgcacttt cctgcactat
    30241 ccgacactgt caccatccat aattcaggct tatctgttta ttacaataac cttatattta
    30301 ttatggattt ttggtgatga tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt
    30361 cgccgctgcc gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc
    30421 gagcagtcgt ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct
    30481 ggaagccatc gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg
    30541 cgttgatcct tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc caaccctact
    30601 tccagacgcg ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta
    30661 cccgccgggt aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga
    30721 cacgctgaca ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac
    30781 gccaacggca aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt
    30841 gccggctgga ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg
    30901 cgtttatttg cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg
    30961 ctgtattcgt ctgcgggatg acgatatcaa aacactcttt agctaggtca ccccaggcac
    31021 caaagtgaat atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct
    31081 ggttgaagta catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat
    31141 tacgctgaat agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat
    31201 gcaacatgtg atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag
    31261 cccacaaact ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga
    31321 aaaaatcagc agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt
    31381 ccatggaggg tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca
    31441 tccgcttttt gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg
    31501 atagtaccga ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg
    31561 gcaaatgaca tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata
    31621 gtggccttat gcagatgaat gacgtcgcaa gaaattactt aacgatcgat aactaaatga
    31681 tagatgtgat ctggatcaca tacaagatat tttttataac aatcattaat taatttaaaa
    31741 atctttaaat ttcagtcaaa tagaaagcaa ttatttttat gggatctgga ctggtgaacg
    31801 gaggtgatta tttttataat aacctttcat tcacagtcac caggtacaac ggcatcatgg
    31861 caactgactc aacacaatgt gtaaaaaaaa gccgtggccg cccaaaagtg ttcgacaggg
    31921 atgccgcgct tgataaggcc atgaaattgt tctggcaaca cggttatgaa gcgacttctc
    31981 ttgcggacct cgtcgaagcg accggagcca aagcgcccac gctatacgcg gaatttacca
    32041 acaaagaggg gttatttcgt gccgttctcg accgctatat cgatcgtttt gccgctaagc
    32101 atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc tgcgctggct gactattttg
    32161 ctgccatcgc caactgcttt accagcaaag acaccccggc aggctgcttc atgatcaaca
    32221 actgcaccac cctctcccca gattcaggag atatcgccaa tacgttgaaa tcacgccatg
    32281 cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg acaagcgcgc ggggaaatcc
    32341 cgacccactg tgacgtgaca catctggcag aattccttaa ttgtattatt caggggatgt
    32401 cgatcagcgc acgcgaaggt gcatcgctgg aaaaactgat gcagattgcc ggaacgactt
    32461 tgcgtttatg gcccgaactg gtgaaataag tcgcaaaaaa agcccctcag cctgagcgaa
    32521 tgaggggcca aattgcagag tacagccttg ttatactttg ttattcaaaa tcagttgccc
    32581 gttactatct agcgggatct gcgtacccgg atcgcggtcc atgcggattt tgccctgctg
    32641 atcgccaatc ttataggtca catcataacc gagcattttt tctgacttgt catacaccgt
    32701 tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc tggagagagc cctggatctg
    32761 gttaccggca tatccaccac ccagcgcccc cacaacagtg gcgacatctt taccgcgacc
    32821 accaccaaac tgatgcccta tcacgccacc agcaacagcg ccgagcaccg acccggtaat
    32881 gcgattttca tcctgcaccg gtcgacgatg ggtcactgtg acgttgcgac actcctgacg
    32941 cggtgtttta accgtttcct tgattggggt tgcagaaaca acctgagcgt attgcgggcc
    33001 ccgttcaaac acgttcagac tggccactgc cgctacgccc aacgcagctg cgacaccaat
    33061 cccgataccc gccaacattg atttattcac gggaacacct ccttctttgc agttatgcca
    33121 atcatgcaag cgaaggagaa aagcagctat cagacagaag atgaaaagcg cgggaatgac
    33181 tggaaatact taaggattcg gaggagtctg aaggaatgcc tgatgcgacg cttaacgcgt
    33241 cttatcaggc ctaccgctcg taccatttgt ccagggcgga taaggcgttt atgccacatc
    33301 agccagtgta cgtcgattaa tgcaacttca aacgcggacg gataacgcgg ttaatactcc
    33361 ccaccagcat cattaatccg gttttaaagt aaccatgcag cgcaatctga tgcattcggt
    33421 atagcgagat atatacaaag cgcgcaattc gtccttcaat catcattgag ccgcgcgtca
    33481 ggttacccat caggctacca acggtggaga agttcgacag cgataccagc gaaccatgat
    33541 ctttatactg ataatttttc agcggcttac cgttcatctg cgccagaatg ttgttcattg
    33601 cgcaagtcgc catctggtgt gcagcctgag cacgcggcgg aacaaagccc ccttccggac
    33661 gcgggcatga cgcgcagtcg ccaatagcgt aaatgtctgg atcgcgagtg gtttgcagcg
    33721 tcggttccac caccagctgg ttgatacggt tagtttcaag accaccgata tctttcagga
    33781 agtctggcgc tttgatcccg gctgcccata ccatcagatc agcctcaata tattcgccat
    33841 ctttagtgtg caggccgcct tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa
    33901 cgccaagttt cgttagctcg ttgtgggccg cagcagagat acgtggcggt aatgcaggca
    33961 aaatacgttc tcccgcttct accagcgtta cgttcagggc ttcgttggtc aggcctttgt
    34021 aaccgtagct gtgcagttgc ttgatcgcgt tgtgcaattc agcggagagt tctacacccg
    34081 tcgcgccgcc gccgacaatc gcaatgttca ctttgccatt tgcgcccagg ttggcagagt
    34141 atttcaggaa cagattcagc atctcctggt ggaagcgacg cgcctggtgc gggttatcga
    34201 ggaaaatgca gttctctttg acgcctggcg tattgaaatc gttagaggtg ctacccagcg
    34261 ccatgaccag ggtgtcatag gcgattttac gttccggaac caacagttca cctttctcgt
    34321 cgcgtagttc tgcaatagtg attgttttcg cttcacgatc aatatcaatg acggaaccca
    34381 actggaactg gaaaccatga ttgcgcgcat gggccagata gctcaacgca tcgacgcctt
    34441 catcaagcga gccagtcgcc acttcgtgca gcagtggttt ccacaggtgg ctgtggttac
    34501 gatcgaccag cgtaattttg gcttttttct tacgtcccag cttatgcccc agctgtgttg
    34561 ccatttccag cccaccagca ccgccgccga caatcacaat ctttttcaat ggcgtagtca
    34621 acgtgacccc cttaaattta ttaaccaatt gttaattaat ggttattaac atagctctta
    34681 attaacaaca ggttacagca atgaaaatgt tcttcgctat tgagaataac atgaatggtg
    34741 cattggtcat accaaaattg atgttaatca agttttgttg cttaaaagat aggcaacttt
    34801 accgggggag atcaaaaaat agcgtttatt atttgagcaa attcggaaga gtaatatagg
    34861 gtgaagcgta tatcgagggc gtttggtcgt cctgataaga tgcgcagcat cgcatcaggc
    34921 attgatgctc gactgtcgga tgcggcgtgg acgccttatc cgacttacag aattcggagc
    34981 gcatcaggta ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac
    35041 gctttaatgc gctgtaaatg cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc
    35101 cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt aagtgcttca
    35161 tcattacgcg acaaaataac cagacaacga tcgcgattct tctcgcggaa gttggtcaca
    35221 cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttgccttc catgttctca
    35281 taagggaaca aattagggtt gaagatcacc tggcggatat cgcaaagaaa accaatccgt
    35341 tccgcccagt atccgcccag gccaacgccg caaattagcg gacgctcgtc aacgttcagt
    35401 tgcaacattt tgtccacttc tttaagcaga tgctgcatat catgtttcgg atgccgcgta
    35461 ctgtagctta tcaagcgtac atccgggtca ataaactgca attgtaagac tttctcgtgg
    35521 ttacccggac tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc ctcacataag
    35581 ccagggttag tgacctgctt tctcttcctg ccagcgttca tgtaactgat tcagacgggt
    35641 gctgatcgct ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg
    35701 atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc
    35761 ccctttacga ttattgcaga ccaggatcat atcgcaaccc gcatccagtg atgcctgccc
    35821 gcgttcggca taactgccca taatcgcggc accttccatc gataaatcgt cagagaaaat
    35881 cacgccgtca aaacccagtt cctgacgcaa aacggttttc agccagtagg gagaaccgct
    35941 cgccggacgc ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt
    36001 attttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgtgg
    36061 acgtggatcg cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa
    36121 gtgtttcccg gtcgttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat
    36181 tgccagggct ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg
    36241 cccgacatcc agcactggcg caaagctgat atcaatatcc atagcgatca tttcgctggc
    36301 catcagccaa cccgcctctt gcgccagttt gccgccctct tccattccca acagcgcagc
    36361 aaaggattgt gctgccggta agcgggtaaa accttcgcga aaacgctgca cgcgtccacc
    36421 ttcctgatct accgccacca ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag
    36481 ttcacgtaac tgggcaggat catgatagtt acgcgtaaag agaatcagcc ctcccaccag
    36541 cggatgcgcc agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat
    36601 tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg
    36661 ccagcctgat aaattgttga tcgccggttt gtcgccagcg gtactcaaac caccctcctt
    36721 tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa
    36781 tcttcgcgcg agtggcatag tcattgacca attgccggtg ctgttcagta ttttccaccc
    36841 acaccgccgc cagttccagc gcgatatcac catctccagc atactcccag tcgatgagtt
    36901 ttaaccctga tgcgctatgc actaaatttc cggcgtggac atccatatgc aatggactta
    36961 agcgtaaagg tcgtggttcc cgcgctttgc gcagacgttt taacattcgc agccaaccca
    37021 cagtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaagggc aacagcgtta
    37081 ttcgccagcc aaaacgtggt tgttgatgta gataatacag caagcctgcc agttcgttgg
    37141 tatccggcaa atacgttttt accgcgccgg gcagatagtc gactaccatc cagtcacgga
    37201 gatataaatg cggcttcggt gcaatgcttg cgggtagttg tgataaagcc cgatactggc
    37261 gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg acgcacaaca aaacgctggc
    37321 cctgatgttc aatgagaaaa ctaccgccac taagcccact attaaacgtc gtgacgggat
    37381 gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa
    37441 acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt
    37501 agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccgaca
    37561 ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac tgtcctgcgg cgacaaacct
    37621 aactgttgct tagccatcga cagttgctgg gcggaaacca gggtaaattt cccgttatta
    37681 gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg
    37741 ttgttaacgc tatcaaccag caggacgcta cccgcagtca ccccgtcagc cccaagcatc
    37801 ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc
    37861 ggtgccgttc gatcttcgtg ctcaattggg cctggctgct gcgggatcgt cggcaccgag
    37921 ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg tttcacttct
    37981 tctaccggtg caggttcacg ttgccccaca cacccggcga gaaacatcgc cagcgcggta
    38041 atcaaggcgt agcgactcat ttttgtcatc aagattcacc cctttacaaa tatagataaa
    38101 gtctgacttt gtgcgccccc agaaaattgg cgctgccgta cagcgttacc gccgaatgtg
    38161 cgggaatggt aacgctgcgt ggcctttcca gaggatgcat ctccagccct ctggcgtcat
    38221 accagtaaaa acgataatga acggtaacgg gttcttgcct ttcgttataa agcgttgagg
    38281 atgctgaagg ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag
    38341 ccaataaaga tgactccatc accagcgatt gctcatcatt caccggaatt tccggatgtg
    38401 aacgacagcc caccagcagt aacaacacca gagacaccag cccaaagcat ccttttctca
    38461 tcgttacaga cctttatgcg ccagcattgg tcccagcgga cggccaccca acaagtgcat
    38521 atggatgtgg taaacctctt gtccgccatg gcggttggta ttcatgatca gacgatagcc
    38581 atcttcggca ataccttctt gctcagcaat ttttgccgct acggtgatca tgcgtcccag
    38641 cgcctgctca tgctcagctg acacgtcgtt cacagtcgga atgaggatat tcggaatgat
    38701 cagaatatgc gttggcgctt gcggcgaaat atcgcgaaac gccgttacca gatcatcctg
    38761 gtagacgata tcggagggga tctcacgacg aataattttg ctgaatatag tttcttctgc
    38821 cacgacattt tcctttttca aatagccatg cgttgcgcca tgctacgccg cgccacactg
    38881 cgagtataga gtatggtcga gttaaacctg ctctttcaac cttaactcac gatttcttaa
    38941 gcaaaaaatc gttttgctgc cgtatatatc gccattattc ccatttctgc ctgcgaatgc
    39001 gctgaaaaga cattctctgt ttggtttaca cgacaaatat gaatgcgtat atttctcatt
    39061 tgcatttgca agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc
    39121 atgagcctga cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg
    39181 gataatcaat atcaagccat caccaaaccg tcactacttg ccggttgcat agcactggca
    39241 ctattacctt ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt
    39301 tcagccacag ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt
    39361 accaaaatgc agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag
    39421 cggatggaag atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc
    39481 agcaaaagtc aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat
    39541 aactatatgg ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca
    39601 ctttctgata tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg
    39661 accgggacgg gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt
    39721 gaatttaaag gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg
    39781 gatttacaaa gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac
    39841 cagaataacg actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt
    39901 gtcgatgctg atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt
    39961 gatgttaata gcccaaccta gggcggttta ccgcgctgga atactgatgg cagcagcaac
    40021 agttacgatc gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac
    40081 aaggtcttta tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc
    40141 acccactctg aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa
    40201 gcggatggta tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc
    40261 ggcaccggtt ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt
    40321 agttatgaat tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa
    40381 aacaatcgtt acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac
    40441 aactttaatg gcaatttccc acaaaccgac tggtcaccac agagcctggc gcaggacgat
    40501 accacacata tgaaatcgtt atatgctgcc actcgtgtca cccttgccga tccgctgcat
    40561 ctgatcctcg gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag
    40621 aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc
    40681 tacgccagct atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat
    40741 ctggctccaa tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc
    40801 cgtctgacca ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc
    40861 ggtacaccta tccccggcag caacggcgaa accgcctata aagcggtgga tgggacagtc
    40921 agtaaagggg tggaatttga actcaacggc gcaattaccg acaactggca gctgacattt
    40981 ggcgcaacgc gctatcttgc agaggataac gaaggaaacg ccgttaatcc taatctgcca
    41041 cgcaccacgg ttaaaatgtt caccagctat cggttgcctg tcatgccaga gttgacagtc
    41101 ggcggtggtg ttaactggca aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc
    41161 ttccgcgccg agcaaggtag ctacgcgctg gtggatctct tcacccgcta ccaggtgacg
    41221 aaaaacttct cgttacaggg gaacgtcaat aacctgttcg acaaaaccta cgataccaac
    41281 atggaaggtt ctatcgtcta cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa
    41341 ttctgaataa cacctgtaaa aaaggcagcc atctggctgc cttagtctcc ccaacgtctt
    41401 acggattagt ggttacggat gtactcatcc atctcggttt tcaggttatc ggatttagta
    41461 ccgaaaatcg cctgaacacc agaaccagca accactacgc ccgctgcgcc cagtttcttc
    41521 aggccagcct gatccacttt agacacatca gcaacgctga cgcgcagacg ggtaatacat
    41581 gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag caaccagagc cggtgccatt
    41641 tcgctggtac ctgttgcttt tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga
    41701 tccagtgctt taatcagcac gcggaagatg gtgtagtaaa caatcgcata accgataccg
    41761 acgatcggga acagccacag tttgctgctg ttaccagaca gaacgatgaa gtcgatcaga
    41821 ccgtgcgaga acgacgtacc gtcacgcatt cccagaagaa tacagattgg gaatgccagg
    41881 cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa cgaacatgaa gaagaactcg
    41941 atcggctcgg tgataccggt caggaacgag gtcagcgccg cggagatcat aataccgccc
    42001 actttcgcgc ggttttctgg tttagcagag tgccagatag caattgcggc agctggcaga
    42061 ccgtacattt tgaacaggaa gccaccagac agtttacccg cagtcgggtc acccgccata
    42121 taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt tggtgtattc accaatctgc
    42181 atctggaaag gtacgttcca gatgtggtgc agaccaaacg gtaccagaca acgttcgatg
    42241 aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag cagcccactg agagaaggtc
    42301 tggattgcag aaccaatcgg cggccaaatg aaggacagca caacgccagt aaagatggca
    42361 gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa agaagccaag atactcaggc
    42421 agcttaatac ggtagaaacg gttaaacatg tacgctgcaa tcgcaccgga gataatccct
    42481 ccgagtacgc cagtatccgc caagtgttta gaggcgattt cttcagcagg taaatgcagt
    42541 accagtggcg caaccacggc catggtttta accatgatgc cataggcaac aactgcggcc
    42601 agcgcggata cgccatcgtt attggtaaag ccgagggcga caccgatcgc aaaaatcagt
    42661 ggcatgtttg caaagacgga accgcctgct tctgccataa catgcgatac aacggcgggc
    42721 agccagctga aattcgcgga accgacgccc agcagaatac ctgcgatagg tagtacggat
    42781 accggcagca tcagcgattt accgaccttt tgcaggttag caaatgcatt cttaaacata
    42841 attgagagtg ctcctgagta taggtgcttt ttttacgttc tcacgcgtgg caagggggga
    42901 gagcctcgcc gtgtacaggg catctaagcg ccctttattt attacacaga gtaaaataat
    42961 tcagtgccaa tttgtttgac ggctatcacg tttcaacttc agatcattgg agaagttgca
    43021 cattttgaca aaacatatgt cacaatattt caaacgcggt tattcaccgc ccaattttcg
    43081 gcggtgaact ttactcgttt tagccattaa ttacgagctt taaaaaaact cattcaacgg
    43141 atggattgaa ggcgggaagc gtcgatgtga aacagacggg cgaagttatc ggtggttacc
    43201 tgcgccagtt cttcaacggc aacacctttc aacacagcca tgtattctgc aacgtcacga
    43261 accatcgcag gttgattctc ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca
    43321 gtttccacca gtaatcgatc cagggggaca taacgcgcag catcgcacag ttgctccgca
    43381 ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc cgagatccag taatttaccc
    43441 gccgtttctc tgtcctctgt aaaacagtgt agtacgccac cgcaatccgt cactttttct
    43501 tcgcgcagaa ttgccagcgt atcggcacgg gcgtcacggg tatggacgat aaccggcttg
    43561 ttcagttcac gaccaatctg gatatgatgg atgaaggact cttgctgacg tactttagtt
    43621 tccggtgtgt aataataatc cagcccggtt tcacccagcg ctacaacacc ctcttctgcc
    43681 gccagacggc gtaaatcttc tacatcgtag ggatcattct ggttgagtgg atgcacgcca
    43741 caagaaaata cgacgttgtc acgttcgcct accagatccc gcatatgtaa ataacccggt
    43801 aatgttgtgg cgactgccag acaaaatttc acatcgcgtg cggcggcttt cgccagaacg
    43861 tcatccacgt ccttatgcaa agattcataa tccagaccat cgagatggca gtgtgagtcg
    43921 actaaaaaca tgatgtctct cttaaagatg aggaaccggt agcacaacgc ccggttgcag
    43981 gtaatgctca atacgcagta aaagatcggt gatgagaagc tcgcggttga tgcctgtgac
    44041 agacattaac tgttcacgaa tgtggcaaac atcccccagt atagcctgca ggcgcgaggg
    44101 agaaagatgg tttgccagtt cggcgaccag gcccggcaca tcaacattgg tcaccagcgc
    44161 agcgccatga tggcgtttta gcgcatccat cagcaacgtt gccacccagt gtaaacgcgc
    44221 cggagcttgt tcatgattaa gggccgccag cagcgaatac cagtcgcccg atggcacgct
    44281 atatgccaac gcctgacaca atgtttcacg agcctgccag ttatctccct gaaacaacgc
    44341 cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga
    44401 cattgtcact tcgcgtgaaa gccaagtcac ggcgtactgt tctggaggcg gcgcaaggta
    44461 atgtaaccga caacgactac gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag
    44521 gaaaaaccag gtttctgctg gtggctcttc aagcgttttc agcaatgcgt tagccgcggc
    44581 gtcggtcagt aaggcagcat cggttaccca gacaactttc gcaccaccta ggcgtgcgtg
    44641 ctcattcagc ttttcggtga cctcacgtac cgcatcaatg cccagcgtat ttttcccttt
    44701 ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc tgcatcaact gacatccacg
    44761 acagtgaccg caacttttgt ggccctgcgg ttgttggcag agtaaataac ggctcagggc
    44821 gtagattaaa gcatcatcgc ccatgcccgg taacgcctga atgagtagcg catggtgacc
    44881 tcttccggcc tgatagctgg ctaccagttt ttcgaaatca ggtcgtaacc atggatacca
    44941 tctcatgcgt ccaactcctt cacccagtgg gtcacggtag tgcggattgc atccatcacg
    45001 gcctccagcg gctgggtggc atcaatggta tgaatacttt tatcttgtgc agccagttcc
    45061 agatagcggg cgcgggtgcg attaaagaaa tcgaaagact cttgctcaat acgatccagc
    45121 tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg taacatcgag atagagcgtt
    45181 aagtcggggc gaaaatcccc gagaacagca tcacgcagtg ttgccagcat atgttggtca
    45241 ataccacgtc ctccgccctg atacgcctga gtggagagat cgtggcgatc gccaatcacc
    45301 caggtgccgt tagccagcgc aggtttgatg accgtttcta ccagttgaac gcgcgcggca
    45361 taaaacatca gaacttcggc tttatcggta atgacttcat cgcctaccga tttgatatcc
    45421 agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc
    45481 atgtcgcgga tacccagttg ctcgagcgtc tcaaccacca cattacgcgc ggtagttttg
    45541 cctgcgcctt ccagcccctc aatgacgata tacttactgc gcattttttt ccttaagcac
    45601 tttcagataa tcctgcacag acttgttatg actggcaaga ttggtattaa acgtgtgacc
    45661 acctttacca tcggccacaa aatagagata cggcgttttt gccggatgcg cagcagcctt
    45721 cagcgaatcc gcccccggcg tagctatcgc acccggcggc aaaccggtaa tggtataggt
    45781 gttatacgct gtcggcgttt ccaggtctgc acgagaaagt ttgccattat aacgctctcc
    45841 catcccgtaa atcacggttg ggtcggtctg caggcgcata ccaatgcgta aacggttgat
    45901 aaataccgag gcaacctgat cgcgttcact ggcaacggcg gtttcttttt cgataattga
    45961 tgccatcgtc accagctgat ttttatcttt ataaggcaga ccgtccgcac gcccttccca
    46021 ggcgctatcg accgctttca ccattttctt gtgagctcgc ttgagtaacg cgacatcggt
    46081 ggtattggcg gtatacatca aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt
    46141 ttccagttca agtgcctgcg ctacggtggc gtacttatcg tcactcagcg tgtgcttgat
    46201 atacggagcc tcacgcaatt gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg
    46261 cagcgggaac tgtgcttctt taccgctttc caacaatttc agcatctcgc gcacggtcat
    46321 ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa agatccggtt cgatacgcag
    46381 cagccattga aaaacccgtg ggcgattgat gatcttatca gcataaagct gttcaccgag
    46441 cgccagacgt ccggtccctg gcttcagggt aaatatcgtc tcttctttga taagcaattt
    46501 gctgtcggca agatggcgaa ccttccagac gcccacacca gcggcgatac ccagtaccac
    46561 cagcaataac aagataatca ataacacttt tttcatgact aattcgggcg ctcacaaagt
    46621 ggggctaaat attcatataa cgttgctgac gaaaaggtga catcgccaca ggcacgtacg
    46681 ggcatcactg gcattaacgc attacaaata accatctcat ctgcctgcaa cgcctcctcc
    46741 agcgaggctt gcacttcgac aagctgataa gaggattgtg ccagtaaacg gatacagaat
    46801 tgtcgcataa tgccgttaac acctgcctga tccagtcgcg gcgtataaac tacattgccc
    46861 ttccgccaga acaaattagc cgcacagcat tccgtaaccc acccttcgct gtcaaggacc
    46921 agcgcctcat cagcgtttgt ctgctcaaga tgagagcgaa tcaatacttg ctcaagccga
    46981 ttaagatgtt taatacctgc aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc
    47041 aacgtcatcc cttcgttacg caaacggtcg taatgtgcag gataagccgt aacggagaga
    47101 atccgcgttg ctggtccgct gttcaatgtg ctgtaccctc gcccgccact accgcgactg
    47161 atcacgactt tcagcacacc attttgctgt tctgctgcca gcgttttcat ctcttgttca
    47221 agctgaggcc agaagtcaca ggaaatcatc aaccgctgac aagcatcctg tagtcgctgg
    47281 atatgcgccg ataacaaact gactttaccg tcgataactc tggcggtggt aaaacaacca
    47341 tcaccaaact gcgttgcccg atcgcttact gccagcgatt cctgcttata accgttaatt
    47401 aagaacatac cggctcctta tcagagaatt gttagtgtgg cagcatgttc actacggaac
    47461 aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga ccttttatat gggtggaaaa
    47521 tgacaactta gatcttttta aagatcaaag aaccattagt gccaccgaag ccgaaggagt
    47581 tacacagcgt gtattccatt ccgctgacct gacgtgcttc atgcggtacg aaatccagat
    47641 cgcaaccttc atccgggtta tccaggttga tggttggcgg aacagcctga tcgcgcagcg
    47701 ccaggatgga gtagatagat tctactgcac ccgccgcacc taacaggtga ccggtcatag
    47761 atttcgtgga gcttaccaac acacggcttg cagcttcacc gaagatggtt ttcaccgcct
    47821 gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg cgcgttaacg tagccaatct
    47881 gactcgcttc aatgcctgca tcacgcagag catttgccat tgccagagct gcgcctgcgc
    47941 cattttctgg cggtgacgtc atatgataag catcgctgct cataccaaag ccgacgagtt
    48001 cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta ctcttcaagt accagcatac
    48061 cggcaccatc gcccagtacg aagccatcac gctctttatc ccacgggcgg ctcgccgctt
    48121 gcgggttatc attgcgggta gataatgcac gtgccgcgcc aaaaccacca acgcccagcg
    48181 gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa
    48241 tacgcgcagc atggccaatg ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc
    48301 ttgggccacg caggccatac atgatagtca gatgacctgc caccatgttc acaatcgttg
    48361 acggaacgaa gaatgggctg atcttacgtg gaccaccgtt catcagagat gtgtggtttt
    48421 cttcgatcag tccgaggccg ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt
    48481 tctcttccgt tatttcaagg ccagaatcct gcatggcctg aacgccagcg acaattccat
    48541 attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa
    48601 aatcctttac taagccagca aatttcgttg cataggcgct agtatcgaaa tggtcgatta
    48661 ggctgatgcc actctgaccg gcaagcagag ctttccaggt agactctacg gtattgccga
    48721 caggagacaa catgcccagt ccggtcacaa ctacacgacg cttagacacg tttgtcctcc
    48781 aaggagggaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    48841 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    48901 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    48961 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    49021 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    49081 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    49141 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    49201 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    49261 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    49321 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    49381 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    49441 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    49501 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    49561 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    49621 cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc
    49681 ttttgacaga cggaaaacag atgaaaggtt agtttcgata atatcgttcc actcttcatc
    49741 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    49801 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    49861 attcaacatc agacctttgc cgttggcacc taaataatca ctgatcgcct gagagccatt
    49921 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    49981 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat
    50041 tttcctcttt taaagctcga gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    50101 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc accttgcccg ggccgacttc
    50161 atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg
    50221 gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac
    50281 atcaacgtta ttcacaacag aaactgttgg tgcgttaaag gtgattttcg ctaattctac
    50341 tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa
    50401 cggcagcgcg cgttttgcgc cagcagcttt acaggcagcg ccagcacgct caaccgcttc
    50461 tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    50521 accttctgca gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc
    50581 cgccatagcg cccgtgcctt ccggtacggc ttcttgcatg aacttaccgc gcatctcaac
    50641 caaacgcacc gcatcagcga aatcaatcac gccagcgcaa accagcgcgg agtattcccc
    50701 caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcgata
    50761 cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    50821 agctggcccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc
    50881 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    50941 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    51001 acgaaccagc gcggagcccc aagtgaatcc accgccaaag gcttcaagca gaaccaactg
    51061 ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc
    51121 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatcgaca taccgagttt
    51181 tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    51241 agaacggtca agattattcg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac
    51301 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    51361 gcggtcggcg tttggcagcg tcagcaattc accataacta ccgtcggcat gcagatgggt
    51421 ggaaatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    51481 aataataata gtcccacgat ctgttggatc gcaggtgcgc gccagtacat cggaaccgac
    51541 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    51601 ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccaacat
    51661 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    51721 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    51781 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    51841 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    51901 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    51961 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    52021 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    52081 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    52141 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    52201 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    52261 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    52321 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aacgtgacat
    52381 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    52441 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    52501 tactgtcaag accctttact tcttcttcac caatattgag caacgccacg cgaggattgg
    52561 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    52621 tgctgtcaca atcgacgttg gcccctaaat cgaggaccac cgttttgccc ttttgctgat
    52681 gtggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    52741 ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    52801 ctttcaccag ctccagggcc atacgcattg aactcccacg actggcgcgg atagcttgcg
    52861 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    52921 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    52981 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    53041 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    53101 ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac
    53161 gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg
    53221 acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc
    53281 gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat
    53341 ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg
    53401 ctttaagctg gctaatacgg caaatgggtt tggcttttgc acttcttcag gcagttcacc
    53461 aaagaccata tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg
    53521 caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt
    53581 aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg
    53641 actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc
    53701 gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc
    53761 gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc
    53821 aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg
    53881 atcgagagtc agggggaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt
    53941 catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg
    54001 cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc
    54061 gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa
    54121 cttattttag cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct
    54181 tttgaatgtg cagcacctga ggtcgacgaa accccacgca gcgacgaatc accgcgacag
    54241 ttggtgcttc gactggcaca agaaaaagcg caatctctgg cgtcacgtta tccggatcat
    54301 ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta
    54361 acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat
    54421 actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt
    54481 gacgtccatt tccgtcacct aagcgaggcg gagattgata attacgtgcg taaggagcat
    54541 cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt
    54601 ttagagggac gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg
    54661 ttacgtcggg aagggaaaaa cccgctgatg ggatagttgt ttgcgccgga tgcggttacg
    54721 tcgcatccgg caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc
    54781 gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagttttt gcaaacaacg
    54841 cttcaaacct tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    54901 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag
    54961 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    55021 ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt
    55081 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    55141 gacacgcacg atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct
    55201 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt
    55261 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta ccagcaacac
    55321 acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    55381 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    55441 ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc
    55501 cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    55561 tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    55621 cacccgcact tcacctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    55681 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat
    55741 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    55801 gccactcatt tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca
    55861 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    55921 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    55981 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    56041 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    56101 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    56161 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    56221 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    56281 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    56341 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    56401 ggcagcgtct gtatgaccta gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    56461 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    56521 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    56581 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    56641 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    56701 gtctggcggg tagctatctg gttctgatgc caaataaccc gcgcgcaggt ggcatttctc
    56761 gccgtatcga aggcgatgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    56821 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc
    56881 aatgggattt aagcttccgc ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    56941 gcccggctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgcc ttccgcgatt
    57001 atttacgtca ggatatcggc gaaatcctta tcgataaccc gaaagtcctc gaactggcac
    57061 gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg
    57121 gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc gccttccagc
    57181 gtgaagtccg tctgccatcc ggtggttcca ttgttatcga cagcaccgaa gcgttaacag
    57241 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa actgcgttta
    57301 acaccaacct cgaagccgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    57361 gcctgattgt tatcgacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa
    57421 accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc
    57481 gctttggcct gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagcc
    57541 atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc
    57601 tctctattct gcgtttgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg
    57661 ccattgttcc tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg
    57721 cgattgaaac gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa
    57781 ccccgcacta ccacgtgttg cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca
    57841 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    57901 gtaagcgtcc tgaacaacct gcactggcaa cctttgccat gccggatgtg ccgccagcgc
    57961 caaccccagc tgaacctgcc gcgactgtcg tagccccagc accgaaagct gcaacggcaa
    58021 caccagcagc tcctgcacaa cctgggctgt taagccgctt cttcggcgca ctgaaagcgc
    58081 tgttcagcgg tggtgaagaa gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga
    58141 aaccggaacg tcaacaggat cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg
    58201 agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc
    58261 gtaatcgtcg ccaggcacag cagcaaactg ccgagacgcg tgagagccgt cagcaggctg
    58321 aggtaacgga aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc
    58381 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag
    58441 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    58501 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    58561 tcgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc
    58621 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    58681 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    58741 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    58801 caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    58861 aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg
    58921 aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc gccgctgtcg
    58981 aacctgttgt tagcgcgcca gttgttgaag aaatggctga agtcgtagaa gcccccgtac
    59041 cggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg
    59101 cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagtcg
    59161 cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg
    59221 ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    59281 tcgtcgctga agtcgcagca gaagttgaaa cggtagccgc ggttgaacca gagatcaccg
    59341 ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg
    59401 aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg
    59461 caggtggtca tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    59521 agtaataatt agctcaaagt aatcaagccc tggtaactgc cagggctttt ttatttcatg
    59581 tttgaatcaa catattaaaa ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata
    59641 ggcaaaaaaa atgcccgccc cggcgatccc agagcgggca gacaaaacat acccagtttc
    59701 atgatatgtt ttaaagcgag cttatttgtt gagctggaag agcgacaatc cctgcatatc
    59761 ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt
    59821 tgcattccag tccacatcaa ccagatcgct catctgctgc gtttgcccta aagcgcgatc
    59881 gctacctaag gaatccagcg actccagttt gttcagctgc gtgcctaatt ccgtgcgaac
    59941 ggtaaggaca ttattcagcg agtttttcag tccacggttg gttttatcca atgccgcagc
    60001 ggcgatttct ttatccgctt cgctatccgc gaccggcgtt ttcagggctg cgatggcgct
    60061 atccagcatg gcaaaaagat tggtttcaga agcgctaccg tctggttccg ctaccgcgtt
    60121 actggtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc
    60181 atcgacctgt tgcttaatac tttctgttcc gccgacataa tcaccgttag cctcactaaa
    60241 cggcgcagtc tctgttttat aaccggcaaa aatgtagcgc ccattaccgt cagtggtgtt
    60301 cgccagattc agcaactggt cacgaagccc ctgaatatcc gtagccagcg aggcccggtc
    60361 atcgtcactc aaggtgccat tgctggcgta gacaattttt tcctgagcat tctggatagc
    60421 agtggtgacc tggctaagta cactctcttc cagtgacact ttttgagtgg cgaaagtacg
    60481 tgccagcgtg tactggctgt tttgcgcctg tgcctgggag agaacaacag cttgtgatgc
    60541 agcaatggga tcgtctgaag ggttaacgac tcgcttaccc gtcgacatct gctcgccgta
    60601 cttcatccat tctgcctgag aattggtgat gccgcgcatg ttttgctggt acatcatctg
    60661 ggtactgaaa cgcattttat ctccccttag cgaatgttaa tcagcgcatc aaaaatcgcg
    60721 tttgccgtct gcagaacctg cgcattcgcc aggtaatact gctgaaaacg ttgcagattt
    60781 ccgtattcct catcgagatt gacaccggaa atcgactgct gctgattgga aagctgcgtc
    60841 accacattac cttgcgtggt gctactggtt ttcaacgtcg cggttttatt accgatatca
    60901 ctcactaacg aggcataagc gtcgttaaag gatttcgcac cgcccaccgt tttactgttg
    60961 ctttgcagat ccagcagggc ctgaccgttg cggttgtcgc tatcacccgc atcttcttcg
    61021 ctcgccatcg cgattttcgc ttcgtcggtg attaatacat ccatgttgac gatggcgtca
    61081 cttactggtt tcagcgtgaa gctgtcgtta acggcaggcg ttcctgtaaa cgtcaactcc
    61141 agaccatcaa atgccacttt accgttggca tctggtgtcg ccgtaaaagt ggtattgctg
    61201 gcaaggcggg tgacctgcca ctgattatta tcgaacgaga ttttgtaatc tgtcgccagt
    61261 accacggagg catcagttac cgtggcaccg atcgcaacgt cacctttgtt tttcgtgttt
    61321 tgctgaaccg cgggattacc gatagtaaag aaatcttcac cagcatcgcc gttagcatca
    61381 aatccggctt tgtgttggct gttgaaagcc tcggcaaatg ccagcgccag ttgtccaagc
    61441 gtattacgcg tctggtccag atcctgagaa cggaatgtca gaatgccgcc cagcgacccg
    61501 gtattcagta atttctccgg gatctcaata ttgcctgccg tcccatcaac gtaagcgaca
    61561 gtcgtacgag aagggtcggc gctggaagga actgccgcca gttgccgcgc cgtacttccc
    61621 tgaaccagtg agtaaccatt ggcatcgtga tgttataagt accgccatcc tgaacgctga
    61681 cttctacgcc aacaatctgg tttaattcgc tcaccagttg atcgcgttga tccagcagat
    61741 tgttaggtga cgcccctgcc cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg
    61801 caatttgttt agcgtaattg ttgatctgat caacgctggc acctatcgcg atattgacct
    61861 gtttgtcctg gtcgcgcaga tattgatcgg tggttttaaa ctgattcacc aacccttctg
    61921 atttcccaat cagcgcctgg cgcgccgccg ggtcttccgc gttactcacc agcgtttgca
    61981 ggctggtgaa gaaatcctgc atctgtgttg ccagcgaaga ggtactggtg gagagcatat
    62041 tgtcgatttt cgacatctgc tcatagcggg cggtcaggcc gctactttgc gtctgcacag
    62101 cacgtaactg gttggtaata aacgtatcat actcacgctg cacaccagaa acgtagacgc
    62161 cattgccaac ccagccgcca gcgcccaacg tgctattggc ctgcgccata atagtggttt
    62221 ggcgggtata tccggcaacg ttatagctgg agatattatt acttgccgta tttaacgccg
    62281 cctgggccgc gttcagtccg ctcatggcgt tattgatcaa gctggacatg gaggttcctt
    62341 ataagccttc aattacagag tattatcggc agcgacccgc cggacttgag ttattcagaa
    62401 cagattatca atgttcatgc tgtaggtttt gctcacctgg tcgcttatcg atttcatctg
    62461 ctggatcatg ttggtgagtt tacgggcata gtgaggatcg gtagcatagc ccgcgtcctg
    62521 taggacctgc gccccctgtt ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt
    62581 acgtgttaac agcccaacgt aatccgacaa tgcttccaga tacgagctgt agacccgaaa
    62641 cttcgctttt actttcttcg cttcgccgtt ttcatattca gtcgtggtga tttcagtgac
    62701 ttgccctttc cagttgccag aggctttgac accaaacagg ttatagcttg gctcgccgtt
    62761 ttcgcggcgg atttgccgtt gcccccaacc agattcaagc gcagcctgag cgaggatcaa
    62821 atgatgtggc acaccgcttt gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag
    62881 gaatgctcta ctgtcacccg gcagcgaatc atcgtagtta cgtggtacgg ccttttgcac
    62941 cagctgcgaa agcgtctggt tttgataacg caccacggtt tcgagcggga atttcatcgg
    63001 tgctgctggc gtggactcct ctggcaatgg ttgttctggc gtcatctgtt taaccatcat
    63061 ctctgcaagc cccagacctt tgcccgccgt catctgttgg gcaatttgct ggtcatacat
    63121 actggtatac agacgagtgt gctcgctgct gaacaggcca tcttttggta aagcgtcgcg
    63181 catgcttttc aacatcatct gcacgaacat cccttccacc tgacgggcca ccggacggat
    63241 atttgccgcc ggatcttcgc tcgctttcgc ctttagttcg ttgagtgatt gcgcatccca
    63301 ggccgcactt gccagtagtt tgctgtcgct gatcatcaga tgatttccag ttttgcccgc
    63361 agacatcccg cactttgcat tgattgcaaa atagacatca gatccatcgg cgtagcgccc
    63421 agcgcattga gcgcacgcac cacgttattg aggctggcgc tggaacgtac gctttgcagc
    63481 gaaccgccgc tctggcgtaa atcgatctgc gtttgtggag taacaacagt ctgtccacca
    63541 ccaaacggtg tatctggctg gctgacattg gcctgacgat taactgttac tgagagattt
    63601 ccctgcgcta ccgcgcagct gtcgagggtc acttctcgat tcatcaccac cgaaccggtg
    63661 cgcgagttaa tcactacttt agcgtcctgc ggggtgacat taacctgcat attctggata
    63721 tcggcaagga aacggacctg ggaactgttg ccacttggta cgcgcacctg aatagtccgc
    63781 gcatctaacg cggtggcgct gccatatcca cgcacgcggt tgatggtgtc agcgatttgc
    63841 tgcgccatgc tgaaatcttc gtcgttaagt tgcaaattaa gggtattccc gacgccaaac
    63901 tggctgggca attcacgttc aataaccgca ccattggtga tccgtccacc gttcagttgg
    63961 ttaacctgaa cactgctacc gcccgcggag gctcctgcgc cgccaaccag aatattgccc
    64021 tgcgccagcg catacacctg actgtcaacg cccttaagcg gtgtcatcaa caacgtacct
    64081 ccacgcaagc ttttggcatt acccatggaa gaaaccacca catcgatggt ttgcccctga
    64141 cgtccaaacg gaggaagtga cactgtcacc attaccacag cgacgttttt tagctgcata
    64201 ttggtgcccg tcggaacggt aattcccagc tgtgagagca tgttattaag cgtttgtgtg
    64261 gtaaacggcg tctgggttgt ctggtcacca gtgccatcca gccccactac caggccatag
    64321 ccaatcagtg agttttgcct taccccctga acactggtga gatcgcgaat acgctcagcc
    64381 tgagccgccg tcgtaaccag tagaagaatt aatgcagaga gaaatttaat caccacagcc
    64441 tcacttacat tggcgacagg ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt
    64501 cgttaatgta gccattgcct acgtattcaa tacgcgcatc cgccacctga gtagacggta
    64561 cggtattgct gccgctgata gtgcgtggat taaccacgcc cgagaagcga ataaattcgg
    64621 taccctgatt aatggcaatc tgtttttcac ccaccacatg caggttgccg ttgaccagta
    64681 cctggtcaac cgtcaccgtc aacgtgccgc taaaggtatt gctggcattg gccccgccct
    64741 ttccgttgaa cgtgttacca ccggaggctt cgacatcggc acgagcgtta ccaaacagcc
    64801 cctgcaaata gcgcggcaca gtatcaaagc caaaattagt tttaccgtca cggctggcat
    64861 tcgcagagga gcttttgctg gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc
    64921 caatattgcg tggtcgacga tcttcaaaca gcggttgata gccatagtta atcggctgag
    64981 cagactggaa aatagaaccg ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg
    65041 tcgccccctg caccagcggc gtggagggta tccaggcgca gccggttagt gaaagcacca
    65101 acaagctgga aatggcataa gtatgcgcag cgtttttttg catggctatc atcttcaaaa
    65161 atcagtaaac cggtgaacct gccaccggtt atgccttaga gttgcgtcag tttttgcagc
    65221 atctgatcgg tggtggacac cgctttactg ttgatttcgt aagcgcgttg cacctgaatc
    65281 atattgacca gttcttccgc cacgttgacg ttagacgttt caacataccc ttgatacagc
    65341 agtcccgcgc cgttcaggcc cggcgtgctt tcgttcggtg caccggagga ttgcgtttcg
    65401 gtgtagaggt tttcgccaat gctctccagt ccggtatcat tcataaaggt ggtgagattg
    65461 agctgcccaa cctgaaccgg agctgcctgg ccttgttggg ttacgctgac cacgccatca
    65521 cgaccgatgg tgatacttaa cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa
    65581 ccaccagccg tcaccagctg cccgttctga tccacctgga aagagccgtc acgggtatag
    65641 gctgatgagc catctggcaa cattacctgg aaaaagccct gccctttaat cgcgacatct
    65701 ttgctgttat tggtctgcga cagattgccc tggctgtgta agcgttcagt ggcgaccggg
    65761 cgtacccccg tgccgatttg taatccggag ggtaaggtgg tttgttcgga agactgtgcc
    65821 cccggctggc gaatggtttg ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa
    65881 ccgttagtac tgacgtttgc caggttgttg gcaatgacgt ccatattggt ttgctgggcg
    65941 tcaaggcccg ttttggcgat ccataatgaa ctgatcatat aatgtcctgt agtggattgc
    66001 tgacaagcag agcagaagca cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc
    66061 aaaggggcga gggaatagtc cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac
    66121 tcatcgacag cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca
    66181 tctgcatttc aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga
    66241 cgttactgcc ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtaccg
    66301 gccctcgcgt ggcctgggtt tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca
    66361 cttcgctgcc cgtggctttc accagtttca gacgccctac tggcgcaact gtatttgccg
    66421 gatcgcccgg attgagcgcc gagattgtgc catcggcagc aatcgtgatt tccgcccctt
    66481 ccggcacagc aattggcccc gcctcgccta tcaccggatg cccctgaatt gtcagttgcc
    66541 cggtgggatc aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggtct
    66601 gcacggccag ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt
    66661 gccgggcgtc atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga
    66721 aagcccttcc accggcactg cgcgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga
    66781 ggcattggcc agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc
    66841 catcgcagta taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa
    66901 cccgcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac
    66961 gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg
    67021 caccgttggt cagggtgcca aagtttcccg tcccggctgt ccccaacagc gccacgccag
    67081 aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa
    67141 agttcgccag tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa
    67201 ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg
    67261 tggttgccac aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcagac
    67321 taaacgtggc gggttctgca ccgttaattg cgccggttgc gatattattc gccatcgcac
    67381 atgtgccatt agcattaaat tccagtgttg tcgctgtctt cgcaatgctg tttggatcac
    67441 tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaaa tagacgctca
    67501 tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat
    67561 ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga
    67621 tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat
    67681 tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca
    67741 gctgtaaacc ttgcatattc aacaggttac ggttttcatc cagcttaaat tgtccgttac
    67801 ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag
    67861 caacgtccag acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac
    67921 cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg
    67981 tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt
    68041 tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa
    68101 atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga
    68161 gatccagcgt attaccgctg ttgccgcgga tcacaccctg caccagagca aactgcagcg
    68221 gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg taagaaccgt
    68281 tcggcgcagt gctgccatca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg
    68341 tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg
    68401 taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc
    68461 cttcttcact gccggttccc gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga
    68521 tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagcg
    68581 tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct
    68641 cgttgttttc cattggattg gtcgggtcct agtttttcag ctgcgccacc agcaaagtca
    68701 gaaaactgct ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac
    68761 tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta
    68821 ttgaccgagc gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt
    68881 ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc tccccgacga catcaacgtt
    68941 cggcattttt acgtagccct ttgcatctgc cagcggatta cctggttcat aaaccagttt
    69001 gtccggggcc tgactttctt aacatcggca acctttacgc cgcctgtcgc agcacctggt
    69061 gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc aggaccggtc
    69121 acgctatcag catttgccag attactggcc gccacgttca ggcgctggga ctgggcagtt
    69181 aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt
    69241 aaaacgttca tcatgccttt gatttgcccg ctcaacgcgc taaggctcat ctggtattgc
    69301 aggctgttat cggcaaactg ggtgacttcc tgacgctggg cgcgcagatt gagcgcctct
    69361 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    69421 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    69481 gtcgctattt atgccgttga tggtcattgc ggacaggtac aactcacgtt gtagaaatgg
    69541 ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    69601 ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc
    69661 gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca
    69721 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    69781 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    69841 tatgtggttg ccgcgatgcc tattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    69901 ctgaaacgtg gacgcctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    69961 gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    70021 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    70081 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    70141 gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    70201 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    70261 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct
    70321 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag ccgcctccac
    70381 cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg atgcaacccg gcagcagtga
    70441 tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt cgtaacggtg aactaaaaat
    70501 ggacaccggc aaaattgccg atgcgctgat caacgaagcg cagcaagact tgcagagtaa
    70561 ctgatcgtat gacacgtctt gcagagatcc tcgaccagat gtccgctgtg cttaacgatc
    70621 tcaaaacggt aatggatcat gagcagcaac atctctctat ggggcagatc aacggcagcc
    70681 agttgcaatg gattacagaa caaaaaagct cactgctggc gacgctggat tacctcgaac
    70741 agttacgcaa gaaagaaccc aatacagcaa atagcgttga tattagtcaa cgctggcagg
    70801 aaattactgt gaaaacgcag caactacgcc aaatgaatca acataacggc tggttactgg
    70861 aaggacagat tgagcgcaat caacaggcgc tggaaatgtt gaaaccgcat caggaaccga
    70921 cgctatatgg ggcgaacggt cagacctcaa caccccatcg cggcagtaaa aagatttcga
    70981 tctgaatatt atctccggcc tgcactgcag gccggaatgc attgttacac cgtccggcgg
    71041 gcaaattctt taactttgaa gcccagtacc gccagtgcag cgaagtacgc ggcaatcccc
    71101 gccagcacga ccgccattaa acgcagtaag cgccagggca tggtacccaa tgaccactcc
    71161 ggcatgatat gtaacatacc taaaagcacg ccagacatca ccagtaccgc caccaccaga
    71221 cgcaacagaa acgccatcca gccgggttgc ggagtaaaga ttttctgctt acgcaactgc
    71281 cagtaaagca gcgaagcatt cagacacgcc gccagaccaa tagaaagtga cagcccggca
    71341 tgtttcaacg gaccaataaa cgccaggttc atcaattgcg ttaaaatcag cgtaacgatg
    71401 gcaattttca ctggcgtttt aatgtcttgg cgggaataaa agccaggagc caacactttc
    71461 actacaatca ggccaatcaa acccaccgag taggcaatta acgcccgctg ggtcatcagc
    71521 gcatcaaacg cggtaaattt accgtactgg aacagcgaaa cggttaacgg acctgaaaga
    71581 atgcccaacg caaccgcact cggcagtgcc aacaggaaac aaagacgcaa cccccagtcc
    71641 atcaaacggt tgtattcatc atgattgcca ctggcaaaac ttttcgacag cgacggcagc
    71701 aaaatggtgc caagcgccac gcccagcaca ccggagggaa actccattaa gcggtcggcg
    71761 taatacatcc aagacaccga accggaagca agaaacgagg caaaaatggt gttgatgatt
    71821 aaggagatct ggctcacaga gacgccaagg atcgccggtc ccatctgttt caccacgcgc
    71881 attgctccag catcgtggaa gttaatgcgc ggcaggacca gcatgccgat cttcttcagg
    71941 tgcggtagct gatacaccag ctgcaggatg ccgccgaccg taacagccca cgccagcgcc
    72001 agcaccggtg ggttaaagta cggtgcggca aacagcgcga aaccaatcat gctgatgtta
    72061 agcagtgttg gcgcaaacgc cggaatcgag aagcggttcc acgtattcag aatcgctccc
    72121 accagcgacg ccagggagat cagcaagata tagggaaagg taatctttag taggtggctg
    72181 gtcagggcaa atttgtcagc tgtgtcagcg aagcctggcg cggtcaccat gatcacccac
    72241 ggtgcggcga gcatgccagc gaccgtcaca accgccagcg caagtgtcag caggccagaa
    72301 acataagaga caaagacccg cgtggcgtct tcaccctgct tacttttata ttccgccaga
    72361 atcggtacaa atgcctggga aaatgcccct tcggcaaaga tacggcgtaa caagttagga
    72421 agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg cgccaaagat tctggcgaca
    72481 attgcgtctc gtgcgaagcc aagcacacgc gaaaacatgg tcatcgagct gacggcggcc
    72541 agcgatttta ataaattcat cggtgttcta atccggaccc acgggcaaaa cgcctgcaag
    72601 gcaggcgctg aaagaggcga atagtctacc tagattatgg tgaattgcta ccgccagatg
    72661 ttacagggtt tattcactca tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag
    72721 cactgcctat tcgccggcgg tttgcggaac tgtctgattt tgcacgcatt caatgaagtg
    72781 ccgcgcacag ccgacaaatc cacgttgctc aagagtgctc tgccaaccag gaatcggttt
    72841 attcactaca ccctgcccgc gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag
    72901 cgcaccgtca gtcacggcct gcacggtttc acgctgactt ccggcacggc gatgcataca
    72961 agtggtgatc tgcaaaggac cggccgaaaa atggtgctcg gcaaacagca tttcgccagc
    73021 gtcgttagtc agtagcgtac cgccatccag agaggctttg ccgcccgaca accacagcgc
    73081 ggtatccacc acatgcagat aatcatccag caacgtgaaa taaagatcgt gtggcccgac
    73141 gctattgcta cgatgtttat ccattcttag cgaggctgcg gtggcgagtt gcgtttttaa
    73201 ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc atcaacgtca gttttttccg
    73261 cgccgccagt tccaccagcc tttcagcatc gcgcagattt tctgccagcg gtttatcgac
    73321 acagacatgt acccccgcat tgagtaacgt actgaccacg tcaaagtggc tggcggtgct
    73381 ggagtgcaca aaaaccgcat cacaactggc ggcaaggctg gataacgaat cggcataagg
    73441 aatgcgccag ctgtcacaaa ttggcagggc tttggcgcgc gtaggcgacc aggctccttg
    73501 taacgtccag tcagacgctg ccgccagcac cggtaaccac gctttttgcg caatgccgcc
    73561 taatcccact acgccgatac gtaatttttt cactttaatc tcccagatgg gccagcaacg
    73621 aatcaagacg ttgtttcagt tctgccactt cgatttccag ggcttcgacg cgggtctgta
    73681 aatcaccgtc aaccatgttc gacatagccg ttaccgccgg ctgatcttca acctcaccac
    73741 tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa
    73801 aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga ctccacttcc gccatatcgc
    73861 tgaattcata cattcgcgcg gcgcggctgc gcagttcacc cagcgtctgg gcaccacgca
    73921 ataacaacgt ggtgattaac gccacttctg cggcgctcag tttcagatcg ccaaattctg
    73981 aattacaaaa acgttgctca tatttggtga cccgattacc aaaaccgctc actgtgcgta
    74041 gataatgacg tttgaccaga ttatccagct gttcctgcac ttcggattca ctcagattca
    74101 tgaccggttc acggttcgtt ttctgattac aggccgtgac tacaccattg actgagagcg
    74161 ggtattgctc cggcgtggtc acctgctttt ccagcaagca accaattacg cgagcttcca
    74221 gggcagtaag ttgatatttc atgagattct ccttagcggc cgggcgtcca gtctggggta
    74281 gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa tcaacagata gtctttcgca
    74341 tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc
    74401 atataattag ccataatgcg atgaatatgt tgggtgcgct gcatataacg aatggctgcg
    74461 gtcagggctt caaacatgag tcctttgccc tgccattttt gcccaatcga ataaccgaga
    74521 tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat tggcaacgcc aataatctct
    74581 ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac cttgtttatg aaattcgtta
    74641 atcatcccta gcctggcctg ccagcctgat ggataacagt ggctttcgtc gcgcactggc
    74701 tcccagggct tgaggaaatg gcgattctct gcgtaataat ccgccagacg ccaggcatca
    74761 cgatcatgca ccagacgcac gaccagtcgg tctgtggtta agcgcacttt tggcacgtta
    74821 ctgcgatagc caaacattct atacctactc cttcccgtaa cgtctttaat acgctgtaag
    74881 tattactata cctgcgcaac cagaccctgt gaaaagtcca aaatgccatt ttctgcatta
    74941 ttcacatatt tgataaaaac tctctatcaa agcgacgttt tgataaaaaa atattgtccc
    75001 cgaagtgatg ggaaaagtct tagcggcaat gcagaataga aagtgctcat cttttatttc
    75061 ccgggagggg aaatgtcccg cgtatcgcag gcgaggaacc tgggtaaata tttcctgctc
    75121 atcgataata tgctggtcgt gctggggttc tttgttgtct tcccgctgat ctctatccgc
    75181 ttcgttgatc aaatgggctg ggccgccgtc atggtcggta ttgctctcgg tctacgccag
    75241 tttattcagc aaggtctggg tattttcggc ggcgcaattg ccgaccgctt tggtgccaaa
    75301 ccgatgattg ttaccggtat gctgatgcgc gccgccggat tcgccacaat gggtatcgcc
    75361 cacgaaccgt ggctattgtg gttttcatgc ctgctctcgg gactcggcgg cacgttgttt
    75421 gatccgccgc gttcggcgtt ggtggtgaaa ctaatctgtc cacaacaacg tggtcgtttt
    75481 ttctcgctgt tgatgatgca ggacagtgcc agtgcggtca ttggcgcatt gttggggagc
    75541 tggctgttgc aatacgactt tcgcctggtc tgcgccacag gggccgtttt gtttgtgcta
    75601 tgtgcggcgt tcaatgcgtg gttgttaccg gcatggaaac tctccaccgt acgcacgccc
    75661 gttcgcgaag gcatgacccg cgtgatgcgt gacaagcgtt ttgtcaccta tgtcctgacg
    75721 ctggcgggtt actacatgct ggctgtacaa gtgatgctga tgctgccaat tatggtcaac
    75781 gacgtggctg gcgcgccctc tgccgttaaa tggatgtatg ccattgaagc gtgtctgtcg
    75841 ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc attttcgtct ggaataccgg
    75901 ttgatggctg ggctgttgat aatgtcatta agcatgatgc cggtgggcat ggtcagcggc
    75961 ctacaacaac ttttcaccct gatttgtctg ttttatatcg ggtcgatcat tgccgagcct
    76021 gcgcgtgaaa ccttaagtgc ttcgctggcg gacgcaagag ctcgcggcag ctatatgggg
    76081 tttagccgtc tgggtctggc gattggcggc gctattggtt atatcggtgg tggctggctg
    76141 tttgacctgg gcaaatcgac gcaccagcca gagcttccgt ggatgatgct gggaattatt
    76201 ggcatcttca ctttccttgc gctgggttgg cagtttagtc agaaacgcgc cgcgcgtcgt
    76261 ttgctggaac gcgacgcctg atttgataca cccccctgac tcctccatac tgaaagtgaa
    76321 ctgacagcca aatggaggag tcacgtgaag ctatacattt acgatcactg cccttactgc
    76381 ctcaaagccc gcatgatttt cggcctgaaa aatatccccg tcgaattaca tgttctgctc
    76441 aacgacgacg cagaaacacc cacccggatg gtcggtcaaa aacaggttcc cattctgcaa
    76501 aaagatgaca gccgctatat gccagaaagc atggatatcg ttcactatgt cgataaactc
    76561 gacggcaaac cgttactgac cggcaaacgt tcccctgcca ttgaagagtg gctgcgcaag
    76621 gtcaatggct acgccaacaa actgctgttg ccgcgttttg ccaaatcggc atttgatgag
    76681 ttttctactc ccgccgcgcg caaatatttc gtcgacaaga aagaggccag cgcgggtaat
    76741 tttgccgacc tgctggccca ctctgacggt ctgattaaga atatcagcga tgatttgcgt
    76801 gcgctggaca aactgatcgt caaaccgaac gccgtgaatg gcgaactttc ggaagatgat
    76861 attcagctat ttccgctact gcgtaatctg acgctggtag ccggaatcaa ctggccaagc
    76921 cgcgttgctg attaccacga taatatggcg aaacagacac aaatcaattt gttatcatca
    76981 atggcgattt aatcctgacc cgccgcggca gcctgtattc gcgggttgcc gccgttttca
    77041 gttttttctg ctggcgagtt tatcgctggt gacacatgct gcgttgtgtc aggatattga
    77101 cgcagatttc atgtcgagga ctaccatgaa caagttttta tttgccgctg cattgatcgt
    77161 cagtggcctg ctcgttggct gtaatcaact cacccaatac accatcaccg aacaagaaat
    77221 taaccagtcg cttgcgaaac ataataattt ctcaaaagat atcggtttac ccggcgtggc
    77281 tgacgcccat attgttctga caaacttgac cagccaaatt ggtcgcgaag agccgaataa
    77341 ggttacccta accggagacg ccaatctgga catgaactcc ctgttcggta gtcagaaagc
    77401 gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat aaagaaaaag gtgcgatctt
    77461 cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg gaaaaaatgc aaacggtgat
    77521 gcaaacgttg cttccctatt tgaaccaggc attacgcaat tactttaacc agcaacctgc
    77581 ttacgtcctg cgcgaagatg gcagtcaggg cgaagcaatg gcgaaaaaac tggcgaaagg
    77641 cattgaagtg aagccaggtg aaattgtcat tccgtttacg gattaatgac gagggcgcat
    77701 tcgcgccctt tatttttcgt gcaaaggaaa acatttccgc ttatcctttg tgtccggcaa
    77761 aaacatccct tcagccggag catagagatt aatgactgca ccatcccagg tattaaagat
    77821 ccgccgtcca gacgactggc accttcacct ccgcgatggc gacatgttac aaactgtcgt
    77881 gccgtatacc agcgaaattt atggacgggc tatcgtaatg cccaatctgg ctccgcccgt
    77941 gaccacagtt gaggctgccg tggcgtatcg ccaacgtatt cttgacgccg tacctgccgg
    78001 gcacgatttc accccattga tgacctgtta tttaacagat tcgctggatc ctaatgagct
    78061 ggagcgcgga tttaatgaag gcgtgttcac cgctgcaaaa ctttacccgg caaacgcaac
    78121 cactaactcc agccacggcg tgacgtcaat tgacgcaatc atgccggtac ttgagcgcat
    78181 ggaaaaaatc ggtatgccgc tactggtgca tggtgaagtg acacatgcag atatcgacat
    78241 ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa cctctgcgcc agcgcctgac
    78301 tgcgctgaaa gtcgtttttg agcacatcac caccaaagat gctgccgact atgtccgtga
    78361 cggaaatgaa cggctggctg ccaccatcac tccgcagcat ctgatgttta accgcaacca
    78421 tatgctggtt ggtggcgtgc gtccgcacct gtattgttta cccatcctca aacgcaatat
    78481 tcaccaacag gcattgcgtg aactggtcgc cagcggtttt aatcgagtat tcctcggtac
    78541 ggattctgcg ccacatgcac gtcatcgcaa agagagcagt tgcggctgcg cgggctgctt
    78601 caacgcccca accgcgctgg gcagttacgc taccgtcttt gaagagatga atgctctgca
    78661 gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc tatggcttgc cggtcaacga
    78721 cacattcatc gaactggtac gtgaagagca acaggttgct gaaagcatcg cactgactga
    78781 tgacaccctg gtgccattcc tcgccgggga aacggtacgc tggtccgtta aacaataaaa
    78841 atcgtagctt cctgttgtca ttaggttatt ttacctgtat aaataaccag tatattcaac
    78901 agggggctat tatgcgaatt gaagtcacca tagcgaaaac ttctccattg ccagctgggg
    78961 ctattgacgc cctggctggc gaactttccc gccgtattca gtatgcgttt cctgataatg
    79021 aaggccacgt atcggtacgt tatgccgcag cgaataattt atcggttatt ggcgcaacaa
    79081 aagaagataa acagcgcatt agcgaaattc tccaggaaac gtgggaaagc gccgatgact
    79141 ggtttgtcag cgaataatat gcagtgattt ttgtttttgc cgggtcgccc cggctttttt
    79201 attttactta cgaattttga gtacaaaatt tctacacttc ctcaaaataa ttgcacaata
    79261 tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta aaagcagaaa
    79321 tttatctgtt ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt
    79381 tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg
    79441 gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc
    79501 agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag
    79561 tccgagcagg aagggactga agttggtcag acgctctggt taaccactga tgttgccaga
    79621 caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta
    79681 aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg
    79741 cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag
    79801 agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct
    79861 gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg
    79921 ccaccgcatc aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt
    79981 gaaggcgaaa agtatgtccc gttagtcctc cgcgcgcaaa cgctgtggga tgaactttcc
    80041 cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct
    80101 gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgttgaa
    80161 aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac
    80221 atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg
    80281 atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt caccgcaatt
    80341 cgtcatgacg atgatggcgt aactattgaa acggttgacg gtgagtatca ggcgaaaaaa
    80401 gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc
    80461 gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa gaataaattc
    80521 ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac
    80581 gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt
    80641 gttccgtttg cggaagtggc cagtgatggg tcagaagcct tcccgttctt gcgcaatgta
    80701 ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac
    80761 gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat taccggcctg
    80821 agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa
    80881 gaccaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca ataatcatca
    80941 ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact
    81001 atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg
    81061 caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca
    81121 aatatttaat ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac
    81181 agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat
    81241 taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa
    81301 ttcattctcc gacacgccat tttctttctc tcgcaaattc gtattggatg cacttatgtc
    81361 attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc
    81421 aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga
    81481 tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg
    81541 gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta
    81601 ttcgcccatg actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt
    81661 cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt
    81721 tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg
    81781 tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tctgttatgc acggatttat
    81841 ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc
    81901 gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc
    81961 cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca
    82021 gcacgccttt gttaacttcc gcatacagca tcttggctat agctggttat atggcacctt
    82081 taaagatttc gacggtactt ttacctttga tgaaaaaaat ccggctgccg ataaagtgaa
    82141 tgtgacaatt aacaccacca gcgtcgatac taaccacgcc gaacgcgata aacatcttcg
    82201 cagtgcggat tttctcaata ccacaaaata tccacaggca acattcacct ccaccagcgt
    82261 gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa
    82321 acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg gcggtaaacg
    82381 tgcaggcttt gaggccgaag gcaaaattaa gctcaaagac ttcaatatca agacagattt
    82441 aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta
    82501 agagtgaaat gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg
    82561 aatacccagt gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc
    82621 acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat
    82681 ctcactacaa caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg
    82741 gcagccatca tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg
    82801 cgcgataatc tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc
    82861 aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc
    82921 ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca
    82981 acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc
    83041 gactgctttt ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa
    83101 gtgccccact tcatactcat agtggttacg catgtcgata aatagtgcat cgggatcgtc
    83161 aagcatggcg ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg
    83221 aggatcgtca ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa
    83281 ggatttcccg tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa
    83341 ggcatagagc tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt
    83401 aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata
    83461 taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt
    83521 ggttcgcggt tcgctctcag ccaacatttt ggcttttaac gcgtcgttgg aaatgcggtt
    83581 gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg
    83641 atatagcaat tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa
    83701 ttatttgtta tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc
    83761 gggcgaaaaa atgcgacaat acatacaatt gcccgtatag gttgaaaaac aggattgata
    83821 tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt
    83881 tgggtattgg cgtactttgg ttggtcgtgc aattgcccta cccgattatc taccgcctcg
    83941 gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc
    84001 gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga
    84061 agaatttcga atccgttggc atgggcctga tggaaaccgg tatggcgtgg ttctggccgg
    84121 accgccgaat cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg
    84181 cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag ctgggcgcgc
    84241 ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga
    84301 ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag
    84361 atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccagatc
    84421 atgattacgg cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga
    84481 ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc
    84541 cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc
    84601 cgccactgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca
    84661 tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag
    84721 gcgttccttc acgctattaa atctcccatg ccggatgctt cagaatggca tccggcatta
    84781 ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc
    84841 gggcgcataa ttagtgtgtt tatcttttct tcttatgttc accgcgcctg gcgcaccaac
    84901 agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga
    84961 tcgtcgcctg gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct
    85021 tacccctcta cgttgagcag cttggtgtta ccggccactc cgccctgaat atgtggtccg
    85081 gtattgtctt cagtataatg ggttcctaaa cacatcattt tagatattga atatgagacg
    85141 ggcaatcagc tacatcagat tttcaagtga acgacaactg aaaggggact ctgtacgccg
    85201 acaatctaag ctggttactg actggcttga taagaaccct gagttttact tggattcatc
    85261 cttgagcttt aaagatttgg ggaaaagtgc ttttagtggt aagcacctga aaggtggtct
    85321 aggtgacttt ttaaccgcta tcgagaaagg gcttgttaaa gctggtgata cgctactaat
    85381 cgagagttta gaccgtctgt cacgtcagga tattgatatt gcttctgagc tattacgccg
    85441 tatcttaagg gctggtgtag atgtagtcac actgagcgat ggagaacatt acacacgaga
    85501 aagtttaaaa gacccgttag ccctgattaa aagcattctg ataatgcagc gggcgcatga
    85561 ggaaagtttg aggaagtctg agcgtgtcca agctgcatgg aaccgtaaaa aagaactcat
    85621 ctccgaaggt atcaaggtat ctaggcgttg tcctgcatgg ttaaggctga atgatgaccg
    85681 tcgaacattc acaatcattc ctgataaagt ggaagttgtt aaacgagcat ttgacctgag
    85741 attacagggt ttaagctttt gggctatcac cagaacttta aacgatgaag gtcacttgtc
    85801 attgaatcaa tacacaccca aacaaaaggg gtggtctgat actgctgtaa agaaactact
    85861 cagaaatcgt gctgtgattg gatgtttcac acctgctgga cgtgaagaag ttcagggtta
    85921 ctaccccgca ataatcagtg agtcactatt ctatcgagta cagcaactga acacagggca
    85981 atatggtagg gcttctgtat caagtaatcc gctatc
//
LOCUS       DAJTRQ010000004        82290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000004 DAJTRQ010000000
VERSION     DAJTRQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..429)
                     /locus_tag="OO332_000338"
     CDS             complement(<1..429)
                     /locus_tag="OO332_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR5940943.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDD"
     gene            complement(500..2614)
                     /gene="fusA"
                     /locus_tag="OO332_000339"
     CDS             complement(500..2614)
                     /gene="fusA"
                     /locus_tag="OO332_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5940944.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2711..3181)
                     /gene="rpsG"
                     /locus_tag="OO332_000340"
     CDS             complement(2711..3181)
                     /gene="rpsG"
                     /locus_tag="OO332_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5940945.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3278..3652)
                     /gene="rpsL"
                     /locus_tag="OO332_000341"
     CDS             complement(3278..3652)
                     /gene="rpsL"
                     /locus_tag="OO332_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5940946.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3778..4065)
                     /gene="tusB"
                     /locus_tag="OO332_000342"
     CDS             complement(3778..4065)
                     /gene="tusB"
                     /locus_tag="OO332_000342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5940947.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4073..4432)
                     /gene="tusC"
                     /locus_tag="OO332_000343"
     CDS             complement(4073..4432)
                     /gene="tusC"
                     /locus_tag="OO332_000343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5940948.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4432..4818)
                     /gene="tusD"
                     /locus_tag="OO332_000344"
     CDS             complement(4432..4818)
                     /gene="tusD"
                     /locus_tag="OO332_000344"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5940949.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4818..5540)
                     /locus_tag="OO332_000345"
     CDS             complement(4818..5540)
                     /locus_tag="OO332_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5940950.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5707..6519)
                     /gene="fkpA"
                     /locus_tag="OO332_000346"
     CDS             complement(5707..6519)
                     /gene="fkpA"
                     /locus_tag="OO332_000346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5940951.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6740..6958
                     /locus_tag="OO332_000347"
     CDS             6740..6958
                     /locus_tag="OO332_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5940952.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7007..7597)
                     /gene="slyD"
                     /locus_tag="OO332_000348"
     CDS             complement(7007..7597)
                     /gene="slyD"
                     /locus_tag="OO332_000348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5940953.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7692..7892)
                     /locus_tag="OO332_000349"
     CDS             complement(7692..7892)
                     /locus_tag="OO332_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5940954.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7902..9707)
                     /gene="kefB"
                     /locus_tag="OO332_000350"
     CDS             complement(7902..9707)
                     /gene="kefB"
                     /locus_tag="OO332_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5940955.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9707..10258)
                     /gene="kefG"
                     /locus_tag="OO332_000351"
     CDS             complement(9707..10258)
                     /gene="kefG"
                     /locus_tag="OO332_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5940956.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10389..12302
                     /locus_tag="OO332_000352"
     CDS             10389..12302
                     /locus_tag="OO332_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5940957.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12302..13324
                     /locus_tag="OO332_000353"
     CDS             12302..13324
                     /locus_tag="OO332_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5940958.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13318..13536
                     /locus_tag="OO332_000354"
     CDS             13318..13536
                     /locus_tag="OO332_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5940959.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13590..14459
                     /locus_tag="OO332_000355"
     CDS             13590..14459
                     /locus_tag="OO332_000355"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5940960.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14514..14918)
                     /locus_tag="OO332_000356"
     CDS             complement(14514..14918)
                     /locus_tag="OO332_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5940961.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15220..15852
                     /gene="crp"
                     /locus_tag="OO332_000357"
     CDS             15220..15852
                     /gene="crp"
                     /locus_tag="OO332_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5940962.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15903..17993
                     /locus_tag="OO332_000358"
     CDS             15903..17993
                     /locus_tag="OO332_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5940963.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18060..19280)
                     /gene="argD/dapC"
                     /locus_tag="OO332_000359"
     CDS             complement(18060..19280)
                     /gene="argD/dapC"
                     /locus_tag="OO332_000359"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5940964.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19366..19929)
                     /gene="pabA"
                     /locus_tag="OO332_000360"
     CDS             complement(19366..19929)
                     /gene="pabA"
                     /locus_tag="OO332_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5940965.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19961..20563)
                     /locus_tag="OO332_000361"
     CDS             complement(19961..20563)
                     /locus_tag="OO332_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5940966.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20553..20720)
                     /locus_tag="OO332_000362"
     CDS             complement(20553..20720)
                     /locus_tag="OO332_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5940967.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20825..21397)
                     /gene="ppiA"
                     /locus_tag="OO332_000363"
                     /gene_synonym="rotA"
     CDS             complement(20825..21397)
                     /gene="ppiA"
                     /locus_tag="OO332_000363"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5940968.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21668..22849
                     /gene="tsgA"
                     /locus_tag="OO332_000364"
     CDS             21668..22849
                     /gene="tsgA"
                     /locus_tag="OO332_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5940969.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23111..25654
                     /gene="nirB"
                     /locus_tag="OO332_000365"
     CDS             23111..25654
                     /gene="nirB"
                     /locus_tag="OO332_000365"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5940970.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25651..25977
                     /gene="nirD"
                     /locus_tag="OO332_000366"
     CDS             25651..25977
                     /gene="nirD"
                     /locus_tag="OO332_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5940971.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26103..26909
                     /gene="nirC"
                     /locus_tag="OO332_000367"
     CDS             26103..26909
                     /gene="nirC"
                     /locus_tag="OO332_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5940972.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26928..28301
                     /gene="cobA"
                     /locus_tag="OO332_000368"
     CDS             26928..28301
                     /gene="cobA"
                     /locus_tag="OO332_000368"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5940973.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28548..28715
                     /locus_tag="OO332_000369"
     CDS             28548..28715
                     /locus_tag="OO332_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5940974.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            29010..30347
                     /gene="frlA"
                     /locus_tag="OO332_000370"
                     /pseudo
     CDS             29010..30347
                     /gene="frlA"
                     /locus_tag="OO332_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30368..31390
                     /gene="frlB"
                     /locus_tag="OO332_000371"
     CDS             30368..31390
                     /gene="frlB"
                     /locus_tag="OO332_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5940975.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31440..32270
                     /gene="frlC"
                     /locus_tag="OO332_000372"
     CDS             31440..32270
                     /gene="frlC"
                     /locus_tag="OO332_000372"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5940976.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32267..33052
                     /gene="frlD"
                     /locus_tag="OO332_000373"
     CDS             32267..33052
                     /gene="frlD"
                     /locus_tag="OO332_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5940977.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33152..33883
                     /gene="phoB"
                     /locus_tag="OO332_000374"
     CDS             33152..33883
                     /gene="phoB"
                     /locus_tag="OO332_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5940978.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(34043..35128)
                     /locus_tag="OO332_000375"
     CDS             complement(34043..35128)
                     /locus_tag="OO332_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5940979.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35140..36444)
                     /locus_tag="OO332_000376"
     CDS             complement(35140..36444)
                     /locus_tag="OO332_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5940980.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36456..36747)
                     /locus_tag="OO332_000377"
                     /pseudo
     CDS             complement(36456..36747)
                     /locus_tag="OO332_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
     gene            complement(36758..37636)
                     /locus_tag="OO332_000378"
     CDS             complement(36758..37636)
                     /locus_tag="OO332_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5940981.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37633..38859)
                     /locus_tag="OO332_000379"
     CDS             complement(37633..38859)
                     /locus_tag="OO332_000379"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5940982.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38860..40023)
                     /locus_tag="OO332_000380"
     CDS             complement(38860..40023)
                     /locus_tag="OO332_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5940983.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40107..40469)
                     /locus_tag="OO332_000381"
     CDS             complement(40107..40469)
                     /locus_tag="OO332_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5940984.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40486..41391)
                     /locus_tag="OO332_000382"
     CDS             complement(40486..41391)
                     /locus_tag="OO332_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5940985.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41661..42665)
                     /gene="trpS"
                     /locus_tag="OO332_000383"
     CDS             complement(41661..42665)
                     /gene="trpS"
                     /locus_tag="OO332_000383"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5940986.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42658..43416)
                     /gene="gph"
                     /locus_tag="OO332_000384"
     CDS             complement(42658..43416)
                     /gene="gph"
                     /locus_tag="OO332_000384"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5940987.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43409..44086)
                     /gene="rpe"
                     /locus_tag="OO332_000385"
     CDS             complement(43409..44086)
                     /gene="rpe"
                     /locus_tag="OO332_000385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5940988.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44104..44940)
                     /gene="dam"
                     /locus_tag="OO332_000386"
     CDS             complement(44104..44940)
                     /gene="dam"
                     /locus_tag="OO332_000386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5940989.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45047..46333)
                     /gene="damX"
                     /locus_tag="OO332_000387"
     CDS             complement(45047..46333)
                     /gene="damX"
                     /locus_tag="OO332_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5940990.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46425..47513)
                     /gene="aroB"
                     /locus_tag="OO332_000388"
     CDS             complement(46425..47513)
                     /gene="aroB"
                     /locus_tag="OO332_000388"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5940991.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47570..48091)
                     /gene="aroK"
                     /locus_tag="OO332_000389"
     CDS             complement(47570..48091)
                     /gene="aroK"
                     /locus_tag="OO332_000389"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5940992.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48492..49730)
                     /gene="hofQ"
                     /locus_tag="OO332_000390"
     CDS             complement(48492..49730)
                     /gene="hofQ"
                     /locus_tag="OO332_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5940993.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49642..50046)
                     /gene="hofP"
                     /locus_tag="OO332_000391"
     CDS             complement(49642..50046)
                     /gene="hofP"
                     /locus_tag="OO332_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5940994.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(50036..50476)
                     /gene="hofO"
                     /locus_tag="OO332_000392"
     CDS             complement(50036..50476)
                     /gene="hofO"
                     /locus_tag="OO332_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5940995.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50460..50999)
                     /gene="hofN"
                     /locus_tag="OO332_000393"
     CDS             complement(50460..50999)
                     /gene="hofN"
                     /locus_tag="OO332_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5940996.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50999..51778)
                     /gene="hofM"
                     /locus_tag="OO332_000394"
     CDS             complement(50999..51778)
                     /gene="hofM"
                     /locus_tag="OO332_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5940997.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51898..54450
                     /gene="mrcA"
                     /locus_tag="OO332_000395"
     CDS             51898..54450
                     /gene="mrcA"
                     /locus_tag="OO332_000395"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5940998.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54615..55175)
                     /gene="nudE"
                     /locus_tag="OO332_000396"
     CDS             complement(54615..55175)
                     /gene="nudE"
                     /locus_tag="OO332_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5940999.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55495..57630
                     /gene="igaA"
                     /locus_tag="OO332_000397"
     CDS             55495..57630
                     /gene="igaA"
                     /locus_tag="OO332_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5941000.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57695..58363
                     /gene="yrfG"
                     /locus_tag="OO332_000398"
     CDS             57695..58363
                     /gene="yrfG"
                     /locus_tag="OO332_000398"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5941001.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58374..58775
                     /gene="hslR"
                     /locus_tag="OO332_000399"
     CDS             58374..58775
                     /gene="hslR"
                     /locus_tag="OO332_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5941002.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58800..59678
                     /gene="hslO"
                     /locus_tag="OO332_000400"
     CDS             58800..59678
                     /gene="hslO"
                     /locus_tag="OO332_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5941003.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59813..61537)
                     /locus_tag="OO332_000401"
     CDS             complement(59813..61537)
                     /locus_tag="OO332_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5941004.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61916..63538
                     /gene="pckA"
                     /locus_tag="OO332_000402"
     CDS             61916..63538
                     /gene="pckA"
                     /locus_tag="OO332_000402"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5941005.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63614..64966)
                     /gene="envZ"
                     /locus_tag="OO332_000403"
     CDS             complement(63614..64966)
                     /gene="envZ"
                     /locus_tag="OO332_000403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5941006.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64963..65682)
                     /gene="ompR"
                     /locus_tag="OO332_000404"
     CDS             complement(64963..65682)
                     /gene="ompR"
                     /locus_tag="OO332_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5941007.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65910..66386
                     /gene="greB"
                     /locus_tag="OO332_000405"
     CDS             65910..66386
                     /gene="greB"
                     /locus_tag="OO332_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5941008.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66483..68804
                     /locus_tag="OO332_000406"
     CDS             66483..68804
                     /locus_tag="OO332_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5941009.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69242..69469
                     /gene="feoA"
                     /locus_tag="OO332_000407"
     CDS             69242..69469
                     /gene="feoA"
                     /locus_tag="OO332_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5941010.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69486..71807
                     /gene="feoB"
                     /locus_tag="OO332_000408"
     CDS             69486..71807
                     /gene="feoB"
                     /locus_tag="OO332_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5941011.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71807..72043
                     /gene="feoC"
                     /locus_tag="OO332_000409"
     CDS             71807..72043
                     /gene="feoC"
                     /locus_tag="OO332_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5941012.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72246..73124
                     /gene="rpnA"
                     /locus_tag="OO332_000410"
     CDS             72246..73124
                     /gene="rpnA"
                     /locus_tag="OO332_000410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5941013.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73197..73466
                     /locus_tag="OO332_000411"
     CDS             73197..73466
                     /locus_tag="OO332_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5941014.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73460..73825
                     /locus_tag="OO332_000412"
     CDS             73460..73825
                     /locus_tag="OO332_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5941015.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73826..74614)
                     /gene="bioH"
                     /locus_tag="OO332_000413"
     CDS             complement(73826..74614)
                     /gene="bioH"
                     /locus_tag="OO332_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5941016.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74652..75335
                     /gene="gntX"
                     /locus_tag="OO332_000414"
     CDS             74652..75335
                     /gene="gntX"
                     /locus_tag="OO332_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5941017.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75394..75969
                     /gene="nfuA"
                     /locus_tag="OO332_000415"
     CDS             75394..75969
                     /gene="nfuA"
                     /locus_tag="OO332_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5941018.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76330..77634
                     /gene="gntT"
                     /locus_tag="OO332_000416"
     CDS             76330..77634
                     /gene="gntT"
                     /locus_tag="OO332_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5941019.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77679..79763)
                     /gene="malQ"
                     /locus_tag="OO332_000417"
     CDS             complement(77679..79763)
                     /gene="malQ"
                     /locus_tag="OO332_000417"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5941020.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79773..82166)
                     /gene="malP"
                     /locus_tag="OO332_000418"
     CDS             complement(79773..82166)
                     /gene="malP"
                     /locus_tag="OO332_000418"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5941021.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19699 a        22374 c        20787 g        19430 t
ORIGIN      
        1 gtcatcaacc atgtcgcatt tgttcaggaa cacgatgatg tacggaacgc ctacctgacg
       61 acccagcagg atgtgctcac gagtctgcgg catcgggccg tcagtcgcag caactaccag
      121 gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc
      181 cgggcagtct acgtgtgcgt agtgacgggt cggggtgtca tattcaacgt gagaagtgtt
      241 gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga atgcacgagc
      301 agcaccgccg taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc
      361 gtggtcaacg tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc
      421 tttagacacg gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact
      481 ttggtattaa cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac
      541 tcggcgcttc atcatacttc aggaattcca tagtgtatga tgcacgacct ttggtcagag
      601 aacgcagctg agttgcgtat ccgaacattt cagacagcgg tacttcagcg tggatcttaa
      661 cgccagtaac ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga
      721 taacgtcacc ggtgttctct tccggagttt ctacttcaac cttcatgatc ggctcaagca
      781 gaactggttt cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg
      841 ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta
      901 ctaccgggta gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg
      961 ccgggatgta ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt
     1021 tcgggtttga acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac
     1081 gaccaccaga ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt
     1141 cacggtaagc aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac
     1201 ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc tggttagatt
     1261 cttcgtcagt ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac
     1321 ccattttttc ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag
     1381 ggaattccat acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg
     1441 ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc gcgaacttct ttgatctctt
     1501 cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg
     1561 agttcagtac ggtatcacca gagttaacca caccggagta aacacggaag aaggtcaggt
     1621 taccaacaaa cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac
     1681 ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta
     1741 cgtcaaccgg ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt
     1801 tcttgaacgc agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca
     1861 gagcaccttt gatttctgct tcagtcagtt cttcaccacc caggtatttt tccatcagct
     1921 cttcagaagc ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa
     1981 ccatgtctgc cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt
     2041 tgatagcttt cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg
     2101 ccagctgcag cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca
     2161 ggaagttcgc acccatgcgg tccattttgt taacgaacgc aatgcgcgga actttatatt
     2221 tgtttgcctg acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa
     2281 ccattaccgc accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt
     2341 gccccggggt gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc
     2401 agaatgcagt agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc
     2461 agtccatggt tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga
     2521 acagaatacg ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatgt
     2581 tacggtagcg tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg
     2641 ttcaatttaa gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac
     2701 tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa
     2761 cgtcttcacg tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg
     2821 ccaggcgcag agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca
     2881 ttgccagagc attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac
     2941 caacgcggcg agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg
     3001 cttccagttc agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag
     3061 attcagcagt agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt
     3121 ctgatccgaa cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca
     3181 tggaaatact ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt
     3241 taagttaaaa cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc
     3301 catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc gcaccacgta
     3361 cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg
     3421 agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag
     3481 tcagacgaac acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat
     3541 atacacgagt acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt
     3601 tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc
     3661 tcctggtttt agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga
     3721 cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca
     3781 ccaggccatc tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg
     3841 aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca ataaggtctt cgttcagggc
     3901 atagacctta atgggggcat tacgcagact ttcaaggtag cggttaccgt caactgcggc
     3961 agttacgcca tcttgcaata atagcagttc gtctccttca ctgagcagac gcagcagcgc
     4021 agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc agcataaacg cctcaaaacc
     4081 tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc atctgcttcg agcggcgtgg
     4141 cttcgacaac aaagggtgtc tgcggatcta acccgcgttc gcgcagtgaa gccgcacaaa
     4201 cccagcactg ttcaatgtcg tacagactca acaatttaaa agtggcaatg taatcacgcg
     4261 ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa aacgccatca gcaataaaga
     4321 agacggccag atcgtcagtt aatgcggaag ttgccagtaa agcatctaaa ccttcccgac
     4381 cagcagctgt accatgaggt gcagtagaaa aaacaaacgc aattcgtttc atcagaactg
     4441 taccaccctg tcacaggtca gcgaggcttc cgccagcgcg ccaagtccgc ttaaggtaaa
     4501 tcccggctga aggtttgacg aagccagccc cagccttcct gcttcagttt catcgacgat
     4561 accgcggcgt aacgctgccg ctacgcagat attcagcgcc acaccatgtt gcgcattcaa
     4621 ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc ggtgaggtca gttggttagc
     4681 gttatagacc ccttcccgat agaaaaagac gctgcttaac ttatggcctt ctgctatcag
     4741 cgcctgcgca aactgaaaag cactactcgc ctgttgcgta ccgtatgctg gcccggtcac
     4801 cacgatggca aaacgcatta cttatcttgc ccctgaaaat caccgctctt gaactggcgg
     4861 atgtagagat agacagtgtg tttggagatg ttcaggcggt cagcaacctg gttgatcgca
     4921 tctttaatat cgaagatccc tttctcgtag agattcagca cgatctgacg atttttggcg
     4981 ttattagaaa cattgcgatc ggcattcact tcttcgatgg tgaactccag cgtttgggta
     5041 accagatctt caacagaaga ggcaaagttg acgcttgaac cgacatccgg ggtttctggc
     5101 ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa gattcatatt gatgcacagc
     5161 agaccaatta cacgctgttc gcggttacga atcgcgatag tcagggactt cattaatacg
     5221 ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc tatccgctcc cgtcatatcg
     5281 tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa tcttccggcc tgtatgttca
     5341 ccgttagcaa tgcgaatggc tgaacatttt agatcctgca aagagtgcaa aacgatttca
     5401 cagtgggagc caataagcat cgctaacccg tccaccaccg cttcgtagga tttcagaata
     5461 tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat ccaactcact ggtttcgttg
     5521 gttaaaagcg acctggacat gaaaaaaaac actccttttc aggagcctgt cgttaacttt
     5581 tcagggcagg ctcattaatg atgcgggtaa ctaaattaat acagcggagg ttccgctttc
     5641 cagcactaat tatatccggc ctgtaataaa aaaaccgccg cctggtcagg cggcggttct
     5701 taatgcttat tttttagcag aatctgcggc tttcgcatca gcttccggct ttgcatcagc
     5761 cttcggcgct ggtttcacat ccagcagctc tacgtcaaac actagggtag aattcggtgg
     5821 gatccccgga acacccgctt tgccgtaagc cagttctggt ggaataacca gtttgatctt
     5881 accgcctttc ttgatgttct tcagaccttc tgtccaaccc gggataacac cgtccagacg
     5941 gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac tctttaccgt cgatcagcgt
     6001 acctttgtag ttcactacaa cagtatcgct gtctttcggt gcttcgcctt taccggcttc
     6061 tactacctga taaaccagac cagttgaaga ggttttcaca cctttctctt tggcaaattt
     6121 ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg tctttttcca tcttcgcctg
     6181 agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct cttggtcgga
     6241 gagtttgctc ttatcagcaa atgcatcctg aacaccagcg atcagctgat ctttatccag
     6301 tttgatgccc agtttttctt gttctttaag agagttttcc atgtaacgac ccagcgaagc
     6361 acccagtgca taagctgatt tctgatcgtc atttttgaac gctgctttgc tgtcagcagt
     6421 tgtagcaggt tttgcagctt cagcagcaaa agtgattggt gcatgcaggg caacggccat
     6481 tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc catatctcca ggatcggggc
     6541 atctcacccc agggttaact attatcagaa gggtactata aagcgttgtc gaacaaatct
     6601 acatacagac acgccctatt atcatctatt ttcagactct ttttgtttaa attagtttcg
     6661 atgaccgcga aatgagtgct gtctcgggca gcaaagttaa gtagaatccg cggcgaccat
     6721 tcgacaaaag aggtgaatca tgcaggattt atcattggaa gcacgcctgg cagagctgga
     6781 gagccgactg gcttttcagg aaatcaccat tgaagaactg aacgtcacgg tgaccgctca
     6841 tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg ctgaccgaga agttaaaagc
     6901 cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg ccaccgccac attattgagg
     6961 cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt tcggtattag tggcaaccac
     7021 aaccgccgtt gcctttaccg ccacagcagc cttcgccacc gtgttcatga ccgtgatcgt
     7081 ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg gtgatgatcg tgcgcgccgt
     7141 gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg aatcgccaca acttcaacgt
     7201 tgaatttcag gttctgaccg gccagcatgt ggttaccatc aaccactacg tgatcgtctt
     7261 caaccgcagt gatttcaacc ggtaccggac cctgctcagt ttcagccagg aaacgcatac
     7321 ctacctgcag ttcatcaacg cccataaata cgtctttagg aacacgttgc actaggtttt
     7381 cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac atcaaatttg tcgccaactt
     7441 catgaccttc cagcgccgtt tccaggccag agatcaggga accgtgacca tgcaggtagt
     7501 ccagcggcgc actcaccgga gactcatcaa ccaacacacc gtcttctgta cgtacctgat
     7561 aggccaggct gaccaccagg tcttttgcta ctttcatgat atctcctgag catgggaaga
     7621 atagtggcgc agattgtagc ggaattctgc agccgtgtac tcactagctt aaaaaaactc
     7681 ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt gctcatcttt gtgaacgtga
     7741 tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc acttaacaca ttcaacaata
     7801 tcaatattat tttcgcgcca catcgccatt gaatcctgcg cctgacaggc cgggcatttt
     7861 gcgcccgcta taaaacgttt tcggattgcc atccttaccc tctactcaaa ttcatcccag
     7921 ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga aaatctcttc cagttcgcgt
     7981 cgggcttccc tggcgcgaga aatttgtacg gtatcagcat gcatcgggat taactcccgc
     8041 agcattcgca tatccaggcg gcgaaaatgc agttgcgccc gctgtgcctg atgcggatgc
     8101 atgccaagcg tgaccagcgt cttgcgcccc agctctaacg cactggagaa tgtttcacgg
     8161 gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc gcgcactcgc
     8221 gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt ccaccagctt catggtgtct
     8281 tccggctcgt tacaggtaat gacgatagac tcagcggcct ctgcacccgc agaacgtaaa
     8341 agatcgacct gcgtggcgtc gccgtaataa actttgtagc catatttacg catcaggtta
     8401 acggcgctga tatcccgctc cagcacggta atgcgcattt tatttgccat cagcaaacga
     8461 ccaatcacct gaccaaaacg cccgaagccc acgacaatga cctggggttt atcatcgttg
     8521 acccacggtt tttcgtcttc ttcttccggt ccgttaaact ggcgggatag ccatttatcc
     8581 accagcttca tcagcaacgg cgtggtcatc atggaaagcg tcaccgtcac cagcaacagc
     8641 gccatctggt cgccctggaa taaacgttgt gaagaagcgg tagaaaagag gacaaaggca
     8701 aactcaccgc cctgactcaa cacgccagca aactgcatcc gctctgagct acgcacgcca
     8761 tacaatcgcg ccagcagata cagcacgaga attttcaccg ccaccagcac aaccacgctt
     8821 atcactaccc acaacagatg ggtataaagc accccgaggt tgagcgacat gccgacagag
     8881 ataaagaaca aaccgagcag caagcctttg aagggatcga tagccgtttc cagttcatgg
     8941 cgatattcac tttccgccag cagcacgccc gcaataaatg taccgagcgc catcgacagc
     9001 cccagcgcat ccataaacaa tgcggaaccc aacaccagca gcagcgtcgc ggcggtgaac
     9061 acttcccgca cgccagaagc tgcaataaag cggaataccg gacgcagtaa atagcgccca
     9121 ccaatcagca tgccgacaaa cgccagcacc ttcatgccga tcttcatcca gtcgaaatgt
     9181 tcgtctgccg accccgccaa caacggcact aacgccagtg ctgggatcac tgccagatcc
     9241 tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc tgcgattcat ccctttctca
     9301 cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa ggccaatgcc accgaccacc
     9361 gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc cgcgcttaac
     9421 agcacctgtg ccacgcctac gccaaaaatc gaacgccgca gttgccaaag tttggaggga
     9481 ttcaactcaa ggccgatgat aaacatcagg aataccacgc cgagttccga aaagtggagg
     9541 atctcatcga cgtcgctaat aaaccccagc ccccacgggc caattgcaat ccctgccagc
     9601 aaatatccca acacagcgcc aatccccagc cgcgatgcca acggcaccgc agccaccgcc
     9661 gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac cttccatcag cggcctcctg
     9721 gagacagcgg atttgccaac cagtcaccgt aggctctggc gtggctcgcc agctcctttg
     9781 cgctttgccg tctcgcccag taaataatga tggaacttaa ccaatgcatc cggcacatgc
     9841 ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg gtagcgattc agcgcgtcat
     9901 aacggtaagc actttccggc tcgccggtgg taatcacgtt acgccagtac tttcccgcca
     9961 gttggtttcc tcccggcccg ctggcaaaac cacgacttaa tacccggtcc agccactctt
    10021 tcagtagcgc cgggcagcta taggtataaa gaggatgctg aaagacaatc acctcgtgct
    10081 cgcgcagtaa tgcctgctca cgggggatat caataaaaaa atcgggatag tgcgcgtaaa
    10141 ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt aagcagtacc cggtttgcca
    10201 ccgagtcctg agattccgga tgggcataca gcagcaaaac tttcgctggc tgagacatca
    10261 tccccctccc ggtatggttt ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt
    10321 gcggcagctc gcccatacat tacattatca taatgataag ttaacatagt ctgaacatac
    10381 ggcgccttat gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg
    10441 ataatgccac cgccaccatc aaccccgggc agaaagtcgg cctggtgggt aaaaacggct
    10501 gtggtaaatc taccctgctg gcattgctga aaaatgaaat cagcgccgac ggcggcagct
    10561 acacctttcc gggaagctgg caactggcgt gggtgaatca ggaaacgcca gcgttaccgc
    10621 aagcggcgct ggaatatgtc attgacggcg accgtgaata tcgtcaactg gaagcgcagc
    10681 tgcacgacgc caacgaacgt aacgacgggc acgccattgc gaccattcat agcaagctgg
    10741 atgctattga cgcatggagt attcgctccc gtgccgccag cctgctgcac ggcctcggtt
    10801 tcagcaatga acaactggag cgcccggtaa gtgatttctc cggtggctgg cgtatgcgtc
    10861 ttaaccttgc ccaggcgctg atttgccgtt cagacttgct gctgctcgac gaaccgacta
    10921 accacctcga tctcgatgcc gttatctggc tggaaaaatg gttgaagagc tatcagggca
    10981 cgctgatcct gatctctcac gaccgcgact tcctcgatcc gatcgttgat aaaattattc
    11041 agatcgaaca acaaagcatg ttcgagtaca ccggcaacta cagttcgttt gaagtacagc
    11101 gcgccacccg tctggcgcag caacaagcga tgtacgaaag ccagcaggaa cgcgtggcac
    11161 atctgcaaag ttatatcgat cgtttccgtg ccaaagccac caaagcgaag caggcccaga
    11221 gccgcattaa gatgctggaa cgtatggagc tgattgcccc cgcacacgtc gacaacccgt
    11281 tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag atggaaaagg
    11341 tcagcgcagg ctatggcgat cgcattattc tcgactcgat taaattgaat ctggtccccg
    11401 gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa atcgacatta atcaaactgt
    11461 tagccggtga acttgcgcca gtcagcggtg aaattggtct ggcaaaaggg atcaagctcg
    11521 gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga cgaatcacct attcaacatc
    11581 tggcacgttt agcgccgcag gagctggaac aaaaactgcg tgactacctc ggcggctttg
    11641 gtttcctggg cgataaagta accgaagaaa cgcgccgctt ctccggtggg gaaaaggccc
    11701 gcctggtgct ggcattaatt gtctggcagc gtccgaatct gctgctgctc gacgaaccga
    11761 ctaaccacct tgacctcgac atgcgtcagg cagtcaccga agcattaatc gagtttgaag
    11821 gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg ttccaccact gacgatctct
    11881 acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat tatcaacagt
    11941 ggttgagcga cgtacaaaag caggaaaacc agaccgacga agcgccaaaa gagaacgcga
    12001 acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc
    12061 agccgctgcg taaagagatt gcccgtctgg aaaaagagat ggagaagctg aacgcgcaac
    12121 tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta tgaccagagc cgtaaagcgg
    12181 agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa
    12241 tggcgtggct ggaagcccag gagcagcttg agcagatgct gctggaaggc caaagcaact
    12301 gatggcgcag ataacgacga ccgatgccaa tgaatttagc agcagtgctg aattcacccc
    12361 tatgcgcggc tttagcaatt gtcatctaca aactatgctg ccgcgtttgt ttcgtcgtca
    12421 ggtgaaattc accccgtact ggcagcggct ggagttgccc gacggcgatt ttgtcgatct
    12481 cgcgtggagt gaagaccccg cacaggcgaa acataaaccg cgtttagtgg tgtttcacgg
    12541 gctggaaggc agtctcaata gcccttacgc ccacggtctg gttgaggcgg cgcaaaagcg
    12601 cggctggctg ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa accgtatgca
    12661 ccgcatttac cattcgggcg aaaccgaaga cgccagttgg tttttacgct ggctgcaacg
    12721 cgaatttggt catgcgccaa cggctgccgt cggctattcg ctcggcggta atatgctggc
    12781 ctgtttgctg gcaaaggaag gcaatgatct cccggttgat gcggcggtga ttgtctctgc
    12841 gccgtttatg ctggaagcct gtagctatca tatggaaaag ggcttttccc gcgtttatca
    12901 gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc aagctggcag cctaccccgg
    12961 aacgctgccg attaatctcg cgcagttaaa atcggtacgt cgcatccgtg aatttgacga
    13021 tctaatcacc gccagaattc acggctacgc cgacgctatc gactattatc gtcagtgtag
    13081 cgccatgccg atgctgaacc ggatcgccaa accgacgctg attattcacg ccaaagacga
    13141 tccgtttatg gatcatcagg tgatcccaaa accggaaagt ctcccctcgc aggtggagta
    13201 tcaactgact gaacatggcg gtcatgttgg ctttattggc ggtacattac ttcatccgca
    13261 aatgtggctg gagtcacgca ttcctgactg gttaacaacg tatctggagg cgaaatcatg
    13321 ttgattccgt ggcaagacct ctcccccgaa acgctggaaa atttaattga aagctttgtg
    13381 ttacgtgaag gcaccgatta tggtgaacat gagcgtacac ttgaacagaa agtcgccgac
    13441 gtcaaacgcc agctacagtg cggagaagcg gtgctggtat ggtcggaact gcacgaaacg
    13501 gtcaatatta tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg cggacgagat
    13561 aatagtgata gctaagtcat ggagttacta tgtctgccaa acatccggtc attgcggtaa
    13621 caggatccag cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa atattcgcgc
    13681 agttaaatct gcatgcagct gaggtggaag gcgacagttt tcaccgttac acccgcccgg
    13741 aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc
    13801 ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat tgaatacggt cagagcggca
    13861 aagggaaatc tcgcaaatat ctgcatacct acgacgaagc cgtaccctgg aatcaggtac
    13921 cgggaacatt caccccctgg caacctttac cagaacccac tgatgtactg ttttatgaag
    13981 gtttacacgg cggcgtagtc acgccacagc ataacgttgc gcagcatgtg gacttactgg
    14041 tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa actgatccgc gacaccagcg
    14101 agcgcgggca ctcacgagaa gcagtgatgg actcggtagt gcgttcaatg gaagactata
    14161 tcaactacat cacaccgcag ttttcccgca cccatcttaa cttccagcgc gttcccaccg
    14221 tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc gctggatgaa agctttgtgg
    14281 tgatccattt tcgtaatctg gaagggatcg atttcccttg gctgctggcg atgttgcaag
    14341 gctcattcat ttcccacatc aatacgttag tggtacctgg cggcaaaatg gggctggcaa
    14401 tggaattaat tatgctgctg ctggtgcaac gattgatgga aggaaagaaa atcgagtaac
    14461 tttgctatta tgccggataa aatactatcc ggcttcacaa cgggatagtt aagtcacgcg
    14521 gcaaccactt catacgagtg agtaatattc accgcttttt ccagcatcaa cgccactgaa
    14581 caatatttct cggcagagag atcaaccgca cgcgcaaccg ctgcgtcttt caggtcgcga
    14641 ccggtgacga taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgt
    14701 tcagaggtca attttacttc acaatcgacc acatcctgac gccctttttg caggatcgaa
    14761 accacatcga tggcactgca accacccgcc gccatcaaca ccatttccat cggacttggt
    14821 gctttatcgc ctgagttgcc gtccattaaa atctggtgac cagaggcgga ttcgcccaga
    14881 aaagttaacc cttcgaccca cttcactcgc gcttgcattt ttgctactcc actgcgtcaa
    14941 ttttcctgac agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg
    15001 ctgaagcgag acaccaggag acacaaagcg aaagctatgc taaaacagtc aggatgctac
    15061 agtaatacat tgatgtactg catgtatgca aaggacgtca cattaccgtg cagtacagtt
    15121 gatagcccct tcccaggtag cgggaagcat atttcggcaa tccagagaca gcggcgttat
    15181 ctggctctgg agaaagctta taacagagga taaccgcgca tggtgcttgg caaaccgcaa
    15241 acagacccga ctctcgaatg gttcttgtct cattgccaca ttcataagta cccatccaag
    15301 agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt taaaggctct
    15361 gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga tcctctccta tctgaatcag
    15421 ggtgatttta ttggcgaact gggcctgttt gaagagggcc aggaacgtag cgcatgggta
    15481 cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg ccaattgatt
    15541 caggtaaacc cggacattct gatgcgtctg tctgcacaga tggcgcgtcg tctgcaagtc
    15601 acttcagaga aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat tgcacagact
    15661 ctgctgaacc tggcaaaaca accagatgct atgactcacc cggacggtat gcaaatcaaa
    15721 attacccgtc aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt gggacgcatt
    15781 ctgaagatgc tggaagatca gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac
    15841 ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta gcgcgccatt ttgtttcccc
    15901 cgatgtggcg cagactgatt tatcaccccg atatcaacta tgcacttcga caaacgctgg
    15961 tgctatgttt gcccgtggcc gttgggttaa tgcttggcga attacgattc ggtctgctct
    16021 tctccctcgt tcctgcctgt tgcaatattg cgggccttga tacgcctcat aaacgttttt
    16081 tcaaacactt aatcattggt gcgtcgctgt ttgccacctg tagcttactg acacagctgc
    16141 tactggtaaa agatgttccc ctgccctttt tgctgaccgg attaacgctg gtacttggcg
    16201 tcactgctga gctggggcca ttgcacgcaa aattgcttcc tgcatcgctg ctcgccgcca
    16261 tttttaccct cagtctggcg ggatacatgc cggtctggga accattgctc atctatgcgt
    16321 tgggcactct ctggtacgga ttgtttaact ggttttggtt ctggatctgg cgcgaacaac
    16381 cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc agattattgt gaagccaaat
    16441 acagcctgct tacccagcac accgatcctg aaaaagcgct gccaccgctg ctggtgcgcc
    16501 agcaaaaagc ggtcgattta attactcagt gctatcagca aatgcatatg ctttccgcgc
    16561 aaaacaatac cgattacaag cggatgctgc gtattttcca ggaggcgctg gatttgcagg
    16621 aacatatttc ggtcagtttg catcagccgg aagaggtgca aaaactggtc gagcgtagcc
    16681 atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg
    16741 ctgatgacat tctttaccat cgcctgccaa cgcgttttac gatggaaaag caaattggcg
    16801 cactggaaaa aatcgcccgc cagcatccgg ataatccggt tgggcaattc tgctactggc
    16861 atttcagccg catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc cgtgacttac
    16921 tggccgataa acagcggcga atgccgttgc ttccggcact gaaaagttat ctgtcactaa
    16981 agtctccggc gctacgcaat gccggacgac tcagtgtgat gttaagcgtt gccagcctga
    17041 tgggcaccgt gctgcatctg ccgaagtcgt actggatcct gatgacggta ttgctggtga
    17101 cacaaaatgg ctatggcgca acccgtctga ggattgtgaa tcgctccgtg ggaaccgtgg
    17161 tcgggttaat cattgcgggc gtagcgctgc actttaaaat tcccgaaggt tacaccctga
    17221 cgttgatgct gattaccacc ctcgccagct acctgatatt gcgcaaaaac tacggctggg
    17281 caacggtcgg ttttactatt accgcagtgt ataccctgca actgttgtgg ctgaacggtg
    17341 agcagtacat ccttccacgt cttatcgata ccattattgg ttgtttgatt gctttcggtg
    17401 gtaccgtctg gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac gcccatgatg
    17461 ctttagaagc ctatcaggaa gcgattcgct tgattctgag cgaggacccg caaccaacgc
    17521 cactggcctg gcagagaatg cgggtaaatc aggcgcataa cactctgtat aactcattga
    17581 atcaggcgat gcaggaaccg gcattcaaca gccattatct ggcagatatg aaactgtggg
    17641 taacgcacag ccagtttatt gttgagcata ttaatgccat gaccacgctg gcgcgggaac
    17701 accgggcatt gccacctgaa ctggcacaag agtatttaca gtcttgtgaa atcgccattc
    17761 agcgttgtca gcagcgactg gagtatgacg aaccgggtag ttctggcgat gccaatatca
    17821 tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg tacgctggag cagcatttac
    17881 agcgggttat tggccatctg aacaccatgc acaccatttc gtcgatggca tggcgtcagc
    17941 gaccgcatca cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg taatacaggc
    18001 taaaccatcg gataaggcat tcacgccgca tccgacatat tttgcctgat gcgtcttatt
    18061 tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccctc atcgatatcc
    18121 gcatcttcca ccaccagcga cggtgctaaa cgcatcacat ccggtccggc attcagcacc
    18181 attacgccaa cctctgcacc cgcatacagg aaatcacgcg cccgaccttt gtagtgtggt
    18241 ttcagctccg cgccaattaa cagccccata ccgcgaatat cgctaaacac atcgtactgc
    18301 tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc
    18361 acttcagggg tattgatgat atcaaacgcc gcgcccgcta ctgcacaggc cagaggatta
    18421 ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag aagcaatttc cgccgtggtc
    18481 agcatggcgc taatcgggaa gccgccgcct aacgctttcg cagaagtcag aatatccggc
    18541 gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc gccccatccc gcactgcact
    18601 tcatcaaaca ccaataatac ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac
    18661 tctggcgtcg ctgccgtcac accgccctcg ccctggatcg gctcaaccac caccgcacag
    18721 gtgtgatcat ccatcaccgc tttcactgca tggagatcgt taaagggaac gtggatgatg
    18781 tctgccggtt gcggcccaaa gccgtcggaa tattttggct gcccacccac cgaaacggta
    18841 aacagcgagc gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacgggcta
    18901 tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg
    18961 gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa tcagtttgcg cccaagacgc
    19021 agcgccggtt cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt
    19081 aacgcgttca ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg
    19141 aaatcgacat actccttgcc ttgctgatcc cagattcggc tgccctgacc ttttaccgga
    19201 ataaactctg ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt
    19261 gctgtttgtt caattgccat ctcatgatca ccctgttacg cataaataaa tatgaaatta
    19321 taatcacaaa atatgcataa aaaatcactg aatggcaatc ggaaatcaga gatgcaggaa
    19381 attagccagc agttgatgtc cttgttcgct aagaatactt tctggatgga actgcacacc
    19441 ttccagatcc cactggcgat ggcgaatccc cataatctcg cgggtttcgc tccaggccgt
    19501 cacgtcaaag cacgctggta atgagtcagg ttccaccacc agcgaatggt agcgtgtcac
    19561 ggtaagtgga tttgccagcc cccgaaatac gccctcaccg ctatgtgtaa tcggcgaggt
    19621 tttgccgtgc atgacctttg cggcgcgcac aactttgccg ccaaatgcct gcgccattgc
    19681 ctgatgaccg aggcagacgc caagaatcgg caagcgcccg gcatagtggc gaataacgtc
    19741 aagggagatc ccggcttcat ctggcgtaca ggggccaggt gagatgacaa ttttttgtgg
    19801 tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc
    19861 ccccagttca caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag
    19921 caggatcatg gcggctccgg tacgaaagaa ccgcactatt ttactcagat tccccggctt
    19981 cgcttaccac tttgctaaat atcatctgca gtgcggtgag atcccccatt gccccactct
    20041 gatttgcctg attccaggcc tctttttcga taccctgcca gctcagttga tatccggcat
    20101 gaatcgccag ttgctcgaag aagatccgct gtgccagacc acttcccacc cggaagggat
    20161 gcagcacgtc gatttcacaa tagtaatgcg ccagccgctc gacgaacttc gctttctcca
    20221 ggccaaccag ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga
    20281 tataagcaaa gtggcagaac ggcgtatcgc cctgataaat atcaacttca cgcagttgcc
    20341 ctgcccagtc gaaaatatcc tggtacagct ggcgatggat agtgcgcaaa tgcggtaaac
    20401 cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg
    20461 cctgttccag ccgctgctgc tggcggatgt tcagccggtt acgcatgata tcaaggccag
    20521 gataaagata cggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct
    20581 tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca
    20641 agacggcgac tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc
    20701 ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa
    20761 gcggcaaatc cgggttacgc cgggagagca gaagcgcggg caagattgcc cgcgcgagaa
    20821 atcattacgg caggacttta gcggaaagga taactaccgg ttttgacggc acattctggt
    20881 acggaccaac atcatgagtc ggcacctggg aaatcttatc ggcaacgtcc atgcctttca
    20941 ccactttacc aaataccgcg taaccgaaat cacgctgacc atggtcaagg aaggcgttat
    21001 cggcaacgtt gataaagaac tggctggtgg cgctgtcttt gtcagcggta cgcgccatcg
    21061 cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc atttttgatt ggcgggtttg
    21121 gttttttctg ctgcatctgc tcggtgaaac cgccgccctg aatcataaag ccaggaatga
    21181 cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac ataatcgaca aagttttgca
    21241 cagacactgg cgctttttgt ttatccagct ccagttcgat gttaccagct gaggttgtca
    21301 acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg agaaagagca gaaagagcga
    21361 aaacagcagc catcgccgcc agggtcgatt tgaacatgag atttccttaa caaacagagc
    21421 agaaaaaaag caagtgaaga gattctaaat agccttcaag aacaaggcca tccctttacc
    21481 taattttacg tatctgaaat atatgtaacc tgacgatcac tttttattcc gtaatcaaaa
    21541 tcacctctta aaatgcaatt tagcaaccga ttgcaataaa acatttaaac agatcacaaa
    21601 atcacctaaa atcgcccgct cgcggcatat cccgctatgc cattttattt ttgaacgcag
    21661 gccaatcatg actaacagca atcgcatcaa gctcacatgg attagctttc tctcctacgc
    21721 actgactggt gcgttggtta ttgtcaccgg gatggtgatg ggaaatatcg ccgattattt
    21781 caatctgcct gtttccagta tgagtaacac cttcaccttc ctcaacgccg gcattttaat
    21841 ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg ttgaaaacgc agttacgttt
    21901 tggctttctc ctgatggtgc tggcggttgc cggtttgatg ttcagccaca gcctggcgct
    21961 gttctcgacg gcgatgttca ttctcggggt ggtcagcggc attactatgt cgattggtac
    22021 attcctgata acacaaatgt atgaagggcg tcagcgcggt tcccgcctgt tatttactga
    22081 ctccttcttc agtatggctg ggatgatttt cccaatgatc gccgcgtttc tactggcgcg
    22141 cagcattgag tggtactggg tttatgcctg catcgggctg gtgtatgtcg ctatttttat
    22201 tctgaccttc agctgtgagt tcccggcact gggcaaacgt gcaccaaaaa cggatgctcc
    22261 ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc tctgttgcgg cgctgtgcta
    22321 catcctcggt cagttaggtt ttatctcctg ggtgcctgag tatgccaaag gcctgggcat
    22381 gagcctgaac gacgcgggca cgctggtgag taacttctgg atgtcataca tggtcggcat
    22441 gtgggcgttc agctttattc ttcgcttctt tgatttgcaa cgcattctga ccgtactggc
    22501 tggtctggct gcgattctga tgtacgtctt taacaccgga acaccagcac atatggcgtg
    22561 gtcaattctc gctctgggct tcttctccag cgcgatctat accaccatca tcaccctggg
    22621 ttcacagcag accaaagtac cgtcgccaaa actggttaac tttgtcctga cctgcggaac
    22681 catcggtact atgttgacct ttgtggttac cggcccgatt gttgaacata gcggtccgca
    22741 ggcggcactg cttacagcaa acggtctgta cgctgtcgtc tttgtgatgt gcttcctgtt
    22801 aggtttcgtc agccgtcacc gtcagcataa caccctgacc tctcattaat tgctaatgcc
    22861 ggacggcact atcgtcgtcc ggccttttcc tctcttcccc cgctacgtgc atctatttct
    22921 ataaacccgt tcattttgtc cattttttgc acaaacatga aatgtcagac aattccgtga
    22981 cttaagaaaa tttatacaaa tcagcaatat accccttaag gagtatataa aggtgaattt
    23041 gatttacatc aataagcggg gttgctgaat cgttaaggta ggcggtaata gaaaagaaat
    23101 cgaggcaaaa atgagcaaag tcagactcgc aattatcggt aacggtatgg tcggccatcg
    23161 ctttatcgaa gatcttcttg ataaatctga tgcggccaac tttgatatta ccgttttctg
    23221 tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg tcttacttct ctcaccacac
    23281 cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag aaacacggca tcaaagttct
    23341 ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag gtgattcact ccagcgccgg
    23401 acgtaccgtt ttttatgaca agctgatcat ggcaaccggt tcctacccgt ggatcccacc
    23461 aatcaaaggt tctgatactc aggactgctt tgtctatcgc accattgaag acctcaacgc
    23521 cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt gttggtggcg gcctgttagg
    23581 tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa acccacgtta tcgaatttgc
    23641 ccctatgctg atggcggaac agcttgatca gatgggtggc gagcagctgc gtcgcaaaat
    23701 cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc cttgagattg tgcaggaagg
    23761 tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc gaactggaag tcgactttat
    23821 cgtcttctct accggtatcc gtccgcgcga taagctggca acccagtgtg gtctggacgt
    23881 tgctccgcgt ggggggattg tcattaatga ttcctgccag acttccgatc cggatatcta
    23941 cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt ggtctggtag caccaggcta
    24001 caaaatggcg caggtcgccg ttgaccatat tctgggtagc gaaaacgcct ttgaaggtgc
    24061 tgaccttagc gccaagctga aactgctggg cgtagacgta ggtggtattg gtgatgcgca
    24121 cggtcgcacg cctggcgcac gtagctatgt ttacctcgac gaaagtaaag agatctacaa
    24181 acgcctgatt gtcagcgaag acaacaaaac cctgctcggt gcggtactgg tgggcgatac
    24241 cagcgactac ggcaacctgc tgcaactggt gctgaacgct atcgagctgc cggaaaaccc
    24301 ggattctctg attctgccgg cgcactcggg tagcggtaag ccgtctatcg gtgttgataa
    24361 actgccggac agcgcgcaaa tctgctcctg cttcgacgtc actaaaggtg atctgattgc
    24421 tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa gctgaaacca aagcgggtac
    24481 tggctgcggt ggctgtatcc cgctggtcac tcaggtactg aacgcggaac tggcgaaaca
    24541 gggcatcgaa gttaacaaca acctgtgcga acactttgct tattcgcgtc aggaactgtt
    24601 ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa ctgctggcga aacacggcaa
    24661 aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg ctgctggcct cctgctggaa
    24721 cgaatacatt ctgaagccgg aacatactcc gctgcaggat tctaacgaca acttcctcgc
    24781 taacatccag aaagacggca cctactcggt gatcccgcgt tctccgggcg gtgaaatcac
    24841 cccggaaggg ctgatggcag tgggtcgtat cgcgcgtaaa tttaatctct acaccaaaat
    24901 caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa ggcgatctgc cggaaatctg
    24961 gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc tatgcgaaag cactgcgtat
    25021 ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt gttggcgata gcgtcggcct
    25081 cggcgtggaa ctggaaaacc gctacaaagg catccgtacg ccgcacaaaa tgaagttcgg
    25141 tgtctccggc tgtacccgtg aatgttcaga agctcagggt aaagacgtgg gtattatcgc
    25201 cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc ggcatgaaac cgcgtcatgc
    25261 ggatctgctg gcagcggata tcgatcgcga aacgctgatc aaatatctcg accgcttcat
    25321 gatgttctac atccgtaccg ccgacaaact gacgcgtacc gcgccgtggt tagaaaacct
    25381 cgaaggcggc atcgattacc tgaaagcggt gatcattgac gacaaactgg ggctgaacgc
    25441 tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta gtgtgtgagt ggactgaaac
    25501 ggtcaataca ccgtccgcgc agactcgctt caaacacttc atcaacagcg acaagcgtga
    25561 cccgaacgtg cagatggtgc cagagcgcga acagcaccgt ccggcaacgc cgtatgaacg
    25621 tatcccggta actctggtgg aggacaacgc atgagccagt ggaaagacat ctgcaaaatc
    25681 gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag gtgacgagca ggtagcgatt
    25741 ttccgcccgt atcacagcga ccaggtgttt gcgatcagca acattgatcc gttcttcgag
    25801 tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg gcgagctgtg ggtcgccagc
    25861 ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt gcatggaaga cgaacagttt
    25921 tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg tgcagctgcg cggttaatgt
    25981 tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc tgtaatgacc ttcggtatat
    26041 tgcaggacat tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc
    26101 aaatgtttac agacactatt aataagtgtg cggctaatgc tgcgcgcatt gcacgcctgt
    26161 cggcaaataa cccgctcggc ttttgggtca gctccgccat ggcgggcgca tatgtgggtc
    26221 ttgggatcat cctgattttc acgctcggta atttgctcga cccgtccgta cgtccgctgg
    26281 tgatgggcgc gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt
    26341 tcaccggaca caccatgttc ctcacctttg gggtaaaagc gggcagcatc agccacgggc
    26401 aaatgtgggc aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtcttcgttg
    26461 ccatgctcta tagctggggc ggcggtagcc tgctgccggt agataccagc atcgttcact
    26521 ccgtcgcgct ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt
    26581 gtaactggct ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat
    26641 ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc cggctacgag cactctatcg
    26701 ctaacatgac gctgttcgcg ctctcctggt tcggcaacca cagcgaagcc tacacgctgg
    26761 cgggtattgg tcataacctg ctgtgggtga cgctgggtaa tactttatca ggtgccgtat
    26821 tcatgggatt gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat
    26881 ttaatcaaac tgaaacggct gccggttaat tactaagggg tttttacgtg gatcatttgc
    26941 ctatattttg ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg
    27001 aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt
    27061 ttattccaca gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat
    27121 ttgatgaaag ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacacgc
    27181 ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac gtagtcgatg
    27241 cgccgaaagc cgccagcttt attatgccgt cgattattga ccgctcaccg ctaatggtag
    27301 cggtctcctc tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat
    27361 cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc cgggcaatta cgcgggcgag
    27421 tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt ctgggagaaa ttgttcgtta
    27481 acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa agccattact gaaacgaccg
    27541 aacagttaat caacgaaccg ctcgaccatc gcggtgaagt ggtgctggtt ggtgcaggtc
    27601 cgggcgatgc cgggctgctg acgctgaaag gactgcaaca aattcagcag gcagatgtgg
    27661 tggtctacga ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc
    27721 gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc gcaggaagag attaaccaga
    27781 tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggt ggcgatccgt
    27841 ttatttttgg ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccgttct
    27901 cggtggttcc gggtattacc gcagcttctg gttgctctgc ctattcgggt attccactca
    27961 cgcatcgcga ttatgcccag agcgtacgct taattaccgg acacttaaaa accggtggcg
    28021 agctggactg ggaaaacctg gcggcagaaa aacagacgct ggtgttctat atggggttga
    28081 atcaggccgc gactattcag caaaagctga ttgaacacgg tatgcctggc gaaatgccgg
    28141 tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc
    28201 aactcggtga acttgctcag caaatgaaca gtccatcgct aattattatt ggtcgggttg
    28261 ttggcctgcg cgataaactg aactggttct ccaatcatta atttaacccg ggccagagaa
    28321 ttctggcctt cttaatgact ctttttatat attcagcaaa taaaacataa ccccttaata
    28381 aatattatct gctaatgggt tatattcacc gttacagcga cttacaaaat taagtcattc
    28441 gctcactgac gccaattaaa tattcctgta tcgttcttaa cgagcagtct gcttatattc
    28501 atataatcaa tgaatattaa ttaatatata atacataggg aatgtaaatg aacaaattta
    28561 ttaaagttgc actggtaggt gcagtactgg ctacgttaac tgcatgtact ggtcatattg
    28621 aaaaccgtga taagaactgc tcttacgact acctgctgca cccggcaatt tctatttcta
    28681 aaatcattgg cggttgcggt cctactgcac agtaagtctc tgacagaaat cggctaacac
    28741 cggtttaatt gcccgacgcc ctctggattt tccgagggcg tatttgtttc tatgtccttc
    28801 ttgcccccgc ccgtaaaaat attttcactt taaattcaat tttataacta catccattag
    28861 ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc acattacaca acatcatatg
    28921 ttgttatatt catcatgcat tgtcatgttg ccttttaaat gactgcaaac tctcccctac
    28981 aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca ggaactccaa cgcaagctcg
    29041 gattttgggc cgttcttgca atcgccgtcg ggacaaccgt cggctccggt atttttgtat
    29101 ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct tacggtgctc gcgtttgtca
    29161 ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc ggaactatcc accgcttatc
    29221 cggaaaatgg cgcagattat gtttatctga aaaatgccgg aagccgaccg ctggctttcc
    29281 tctccggctg ggccagcttc tgggccaacg atgcgccgtc attgtcgatt atggcgctgg
    29341 cgattgtcag caatcttggc tttttaacgc ctatcgatcc gttgctcggt aaatttatcg
    29401 ccgccggatt aattatcgcc tttatgttgc tacacctgcg ctccgttgaa ggcggcgcag
    29461 cgtttcagac gctaattacc atcgccaaaa ttatcccgtt cactatcgtc attggccttg
    29521 ggatcttctg gtttaaagcg gagaattttg ccgcccttac caccactgcg attggcgcaa
    29581 cgggcagctt tatggcgctg ctggcgggga tctctgccac cagttggtcg tataccggca
    29641 tggcctctat ctgttatatg accggcgaaa ttaaaaaccc cggaaaaacc atgccacgag
    29701 cgctgattgg ttcctgtctg ctggttctgg tgctctacac cctgctggcg ctggtgattt
    29761 ccggcctgat gcccttcgac aaactcgcca attctgaaac gccgatttcc gacgccctga
    29821 cctagatccc cgcactcggc agcaccgctg ggatctttgt tgccatcacg gcgatgatcg
    29881 tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc gcggctggaa tacgcgatgg
    29941 cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca tccgaaatac aacacgccgg
    30001 atgtctccat catcctgcaa ggggcgctgg ggatcttctt catcttcgtt tccgatctca
    30061 ccagcctgct gggttatttc accctggtga tgtgtttcaa aaataccctc accttcggct
    30121 ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct gtggcgtact ccggctttcg
    30181 ggctgatgac caccctcgcc attgcgtcaa gcctcattct ggtcgcctca acctttgtct
    30241 gggcaccgat tcccggcctt atctgcgccg tcatcgttat tgctactggt ctgcctgctt
    30301 acgccttctg ggcgaagcgt agccgccagc tcaacgcttt gtcgtaatgt tatctgaaga
    30361 aaataaaatg ttggatattg ataaaagcac cgtggacttt ctggtcaccg aaaatatggt
    30421 tcaggaagtg gaaaaagttc tcagccatga cgttccgctg gtgcacgcca tcgtggaaga
    30481 gatggtgaag cgcgacattg atcgtattta tttcgttgcc tgcggctcgc cactcaacgc
    30541 ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat cttcaggtct acgccatttc
    30601 cggctgggag ttctgcgata acaccccgta tcgcctcgac gatcgttgcg cggtgattgg
    30661 cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg ctggagctgg gccgggcctg
    30721 cggcgcactc actgcggcgt tcaccaaacg cgcggacagc ccgattacct cggcggcgga
    30781 atttagcatt gattatcagg ccgactgtat ctgggaaatt cacctgctgc tctgctacag
    30841 cgtggtgctg gagatgatca ccctcctcgc gccgaacgcg gaaatcggca agatcaaaaa
    30901 cgatctcaag cagttgccga atgcgctcgg tcatctggta cgcacctggg aagaaaaagg
    30961 ccgccagctt ggtgaactgg ccagccagtg gccgatgatt tataccgttg ctgcgggtcc
    31021 gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg atggaattta cctggacgca
    31081 cggctgcgtg attgagagcg gagagttccg ccatggcccg ctggagattg tcgaaccggg
    31141 cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc cacaccaccg aacgcgccat
    31201 taactttgtt aaacagcgta ctgacaacgt gatcgtcatc gattacgccg aaatttcgca
    31261 agggctgcac ccgtggctgg caccgttcct gatgttcgtg ccaatggagt ggctctgcta
    31321 ctacctgtct atttacaaag atcacaaccc ggatgaacgc cgctattacg gtggtctggt
    31381 ggaatattaa tccctctccc ggcccggcaa cgggccggat ttacgcaagg agttacccga
    31441 tgaaaacagg tatgtttacc tgcggccacc agcggctgcc tattgaacac gcatttcgtg
    31501 atgcaagcga gctgggttac gacggcatcg aaatttgggg cggtcgcccg cacgcgttcg
    31561 cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc gctggcgcag acgtatcaga
    31621 tgccgattat cggttacacg ccagaaacca acggctatcc gtataacatg atgctgggcg
    31681 atgaacatat gcgtcgtgaa agcctggaca tgatcaagct ggcgatatat atggcaaaag
    31741 agatgaacgc gggttatacg ctgatttctg cggcccacgc gggctatctc acgccgccta
    31801 acgttatctg ggggcggctg gcagagaact taagcgaact gtgtgagtac gcggaaaaca
    31861 tcggcatgga tctgatcctc gaaccgttaa cgccgtatga atcgaacgtc gtgtgtaatg
    31921 ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc gcgcctgttc agcatggtcg
    31981 acatttgcgc gccgtatgtc caggcggaac cggtgatgag ttatttcgac aaactgggcg
    32041 ataaattacg tcatctgcat attgtcgaca gcgacggggc cagcgacacg cattacattc
    32101 ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga tattattgat cggggctatg
    32161 agggttactg tacggtggag ctggtgacga tgtatatgaa cgagcccaga ctctatgccc
    32221 gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga tgagagatga aaaccctagc
    32281 gacaatcggc gataactgcg tcgatatcta cccgcaactg aataaagcgt tttctggcgg
    32341 taatgcggtc aatgtggcgg tgtactgcac tcgctacggc atacagccgg gatgcattac
    32401 ctgggtgggt gacgatgact acggcacaaa gctgaagcag gatctcgccc gcatgggcgt
    32461 cgatatcagc catgtccata cgaaacacgg cgttaccgca caaactcagg tggaactgca
    32521 cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg atggccgact ttgccctgag
    32581 tgaagaggat tacgcctggc tggcgcagta tgacattgtg cacgcggcaa tctggggaca
    32641 tgcggaagac gcattcccac agctgcacgc tgcgggcaaa cttaccgctt tcgacttctc
    32701 cgacaagtgg gacagcccgc tctggcagac actggtgccg catctcgatt ttgcctttgc
    32761 ctccgcaccg caagaagacg aagcgctgcg tctgaagatg aaagcgattg ttgcccgtgg
    32821 cgcaggaaca gtgattgtca cgctgggtga aaacggcagc attgcctggg atggcgcgca
    32881 gttctggcgt caggctcctg aaccggtgac ggttatcgac accatgggtg ccggagattc
    32941 gttcattgcc ggattccttt gcggctggtc tgcggggatg acattaccgc aggcgatggc
    33001 gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac ggtgcctggt aggtataacg
    33061 ttggcgtgag catcttcacg ccaacgtgct gttacttgcc ggaaaacgac cctataatcc
    33121 gagtaattca ttctttattt cagggtcgat tatgtcagct acggaccgct actctcatca
    33181 actcctctac gctaccgtcc gccagcggct gctggatgat atcgcgcagg gggtttacca
    33241 ggccgggcaa cagatcccta ccgaaaacga gctttgtaca caatataacg tcagccgcat
    33301 taccattcgc aaagccatca gcgacttagt ggcagacggc gtactgatcc gctggcaggg
    33361 aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc ctgcttactg tcagtggttt
    33421 taccgatttt ggcgtctcac aaggcaaggc gacgaaagag aaagtgatcg aacaggaacg
    33481 ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc ggaaacagcg aagtgttcca
    33541 tctctgccgg gtgatgtatc tcgataaaga gccgctgttt attgatagtt catggatccc
    33601 gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa ggaagctcca cctatcagtt
    33661 atttcaggag cgttttgaca cgcgagtggt cagcgacaaa aagaccatcg atatctttgc
    33721 cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg ggcgaaccgt tgtttcgcat
    33781 cagcaaaatc gcctttgatc agaatgacaa accggtgcac gtctccgaac tcttctgccg
    33841 cgccaatcgc atcaccttaa cgattgataa taaaagacat taaccgtagg ccggataaga
    33901 tgcgccagca tcgcatccgg cgatgctggc gcactgaatt ttacatcccg cacgttcccc
    33961 tcaccctaac cctctcccca aaggggcgag gggagcgttc agtgcggaat ttagcttgat
    34021 tgccgaacgg tgctatccag cattaaatac tcgcaatact ctcacgcaaa atccgcagca
    34081 ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc acaatgttcg agttgtggat
    34141 tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg cgggatttca tatttcgact
    34201 ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc ttcttccagc accctggcgg
    34261 caatcagctg atgaaactcg acaatcaaca ccttcgactg cgcattggca atgacggcgc
    34321 ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag caaccgttcg gatactcccg
    34381 cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg caagacttcc agcgcctgtg
    34441 agccctggat ctggctaccg ccggagtaaa gcgtggcgcg aatccgacta ataacatcgg
    34501 catcgccaac cactgcacca acgccctccg gcccaaacag cttgaagcag gagaatgtcg
    34561 agacattcgc gccgcattca cagccgattc gcgctacctt catcaccgca tagttatcat
    34621 cggttaacgc tggaacacct gccgcgcgca acgttgccag cacatctgcc agcacatagc
    34681 tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc atcaggttgt tgctcgtcga
    34741 cgacctgctt cagtgccaac aggtcattga aatcagcagt aataagcgtc agccccatct
    34801 gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc atgcaccaga agacgctgcc
    34861 ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc agtccccgcg ccctgcacca
    34921 gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg ctccacccgt tgggtaatac
    34981 gcggttgatt cagacctgga gttaacccta aatcaccgcc agcaagaaac tcgctgcctg
    35041 gaaaatggcg gcaaatagtg tccaccagcg caaactgctt ttgctgcgct tcagcaagag
    35101 tcaggctttg caaaggaaac gtcttcatag gagccttcct taagccggaa cgaacaggcc
    35161 aagccagtaa agaacattca gcagaatacc ggtgatcatt actgccacga ccggtgccgc
    35221 cattttctgt accggacggc ccagggattc gttgaggaag taaatagcaa ccgcgataga
    35281 gaatccggta taacccgcca tcttaattgc tgcgaaaatc gaaccgacca gcagcgccat
    35341 ttccatcagc atattcatgg cgttacggat gttatccgac gcattacgca ccgacgggta
    35401 gcgtcccagc catttgccga tcgaacgaag cagcaagact tccgccgaaa taaccactgc
    35461 gcctaatacc gctgcaacca tcggattcgg cgagagatag cccaccgcat aaacaaaggt
    35521 aaagcccgca actgcataca cacccgttgc taaggcggtg gtggcaatca acggcacaaa
    35581 ccccagtccg cgcataaatt ctgccagagc cgcctgatta atcagcgttt gcgattgttc
    35641 cggcgtcacg cctgcggaat acgctttctc cagtgtgaag atcgaaactt cgctaccagc
    35701 aaaaatcttc atactggcaa cggcggcaat caatgctccc acgatggcga tataaggtaa
    35761 gtttttgata atccgcgacg tgcgctcttc aaacaccgaa agcccgctgg catcgatgtc
    35821 attttcatca cgatgacgca ggtcgtgagt tatcgcgatc cccagcagca tcaccatgcc
    35881 aataaagatt tcgatggatt cagggttaag atgtgggaaa tagcgcacga ctaccacacg
    35941 ggtcatcaga acaaccacgg cggcgaccag actttgcttc cagccaaact ggtagaaaat
    36001 cgccaccagc gggaacagtg caaaagcaga aaccaccggc gagcttaatt ccccgaggct
    36061 acctaataca tccaccggca gcgcggtcag cagctggtta actggcaaca ggcaagtaag
    36121 gatcaacacg ccccagatag cgccaagacc aaacgccatc aggctgttta tcgccagtac
    36181 gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg agcagccagg ttttcagggt
    36241 aaaagagatc cccaccgagg ccacaaaacc aatgctcaga ccaaaagcga tactccccgc
    36301 ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg atcgggtgga tgccgtcatg
    36361 aaaaacagcc gccgagcgat gcgccagaag cgatgtcata cccgtcaggc acgccaccac
    36421 gataatctga atatacagat ccatagtcgt gccttttatt tcagatggtt aatcaacatc
    36481 ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg ccactttccc ttcagcgatc
    36541 attttggcga tatgctcgtc tttcgcttta tgccgcgccc gcaccggtat tacacgcgcc
    36601 aatgtaataa tctagttgcc cggatttcac cttcattgcc gcttccatgt cgttgtgaat
    36661 gaaaacttca aaacagccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg
    36721 taagcctgca acgccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggat
    36781 tttcacgtaa catcacatcc acatcggcct gactgaatcc tgactggcac aattgcggaa
    36841 taaaggtggt caataaatag tcatagccat aaccaccatt ggcttttaaa tgggagcggc
    36901 gcgtaatatc catcgacagc atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga
    36961 gcatcgcaat gcgcttttcg tccgggtagt aactgttctt accaatggtg tcgaactgca
    37021 cgtacgcgcc gagatcgatc atcttcaaaa tgttgtcgag gttgtctttc agatcgcagt
    37081 gaccaacggt gacgcgcgaa agatcaactc cgtgggcttg caacagagcc agttgttcca
    37141 gccccatggt gctgaacgac gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca
    37201 gcgcagcggc aataaatacc ttctcttcca gcggcgtaat ctttccttcg ctggagccga
    37261 tctccgcgat gatcccggct ttcagctccg tgccatcgat accctgttca atttcatcga
    37321 ccatctcctg cgccagttcc tgcacgctgc gggtcaccac atgttccggg aaaaacgcat
    37381 cctggtaata accagtacag gccaccacgt tgatcccggt ttcgcgcatt acatcgagca
    37441 taaattgcgc attgcgcccc atgtaacggt tggtcatctc aatcacatta cgtacacccc
    37501 gggtcatcag gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca
    37561 cgttgttttt aaagccggag agatcaatat gcagatgctc atgggccagg gtgtaacccg
    37621 tcggatcaaa actcataggg tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc
    37681 attttgtggc ggtggcgcac ggaaaaattc acacacggta gctcccacat cagaaagcgt
    37741 ggtgcgcaca ccgagctgcg tggcgaccag cccttgctga taaaccagca ctggcaccac
    37801 ttcacgggta tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac
    37861 caggcaatca tctggctgca tcgcctcaac aagccgggca aggttacggt caacgacctg
    37921 caaacgttcg gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat
    37981 attgatgcaa ataaacgccg tcggatgggt gttaaattcg tcgagggtga tatccataat
    38041 ccgctggcta tccaccaggt tttgccagct cacaccataa ggattgctga cgatatctgc
    38101 caccttaccc accagcacgg ttggcacgcc cgcttcatac agtttttgcg gcacctgcac
    38161 tttttcatca acaccgtagc ccatatgcac gacctggaag ccgttgtcat aagcgccaga
    38221 acgcggcgca ttaataccaa taaagcgccc ttctttgctt tctgcggcat cgagaatacg
    38281 ctgactgttg gttaacaggc cgccaaatgt aatgacccga ccgacctgta cctggtcacg
    38341 cacgatacga ccaattttga ttgcgtcgtc aaaagagatc acagagagat tggcggtaat
    38401 gttatagact tggcctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac
    38461 ccacagaaat tgcagatcat cgccacggcg ctccacctgc cagccagcgg aaactaatgc
    38521 ctgctcaaca cggtcaatca catcgcgaaa aggcatccgc agcggcggta acgggcgcgt
    38581 gcctaaaatt tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac
    38641 gccccaggtt gcggaatctg acggctgcat atcgcctggc gcataaccca atgtgttgat
    38701 tagccccagc ttctccaacg ttggtagctg caaatgcggc aactggctca ggatgtgacc
    38761 acaggtattc gccccggcat cttgcggacg caccagcgtg acatctttca ttgcccctac
    38821 gccaaagcta tcaatcacta acaccacaaa tcgcgccatt tatgccttca gagggttgcc
    38881 gaggctgtca taacgaccaa ctatttctgg ttcgccgcgc tgaataccgg acaccagcac
    38941 cacatcgcta cgggtgacaa aaatctgcgt gcgaaaacag agcaccactg cactgctcac
    39001 cggatactcg cctgccagcg gcagggtata gtcgatactg ctgtcatcca cggttttgag
    39061 attggtttca gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcatgacc
    39121 acgacggtag taaccgccgc cgtagcagta gctgtcgcca cggaaatgat gggagatttc
    39181 acttaaccag agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt
    39241 accggtcagc gcatgaccgg gttcggcatg agtcgcaccg tattccgcca gcaatggcag
    39301 cgaagtgcag ctggtcgctg aaggtacgtt cagttgctca atcgcaatac cagatttcgc
    39361 cagttgatcc cgtgcttgta ccagcgtgtg aagattcggt gttggcaaaa ctttcccggc
    39421 agcctcatcc caaagcaggc aagggaaatg ggttagtccg gctaaatgca gccccggcag
    39481 tttcttaatt tcagcaatca cctcgtgcag cgaatgctgt acaaaaccgc tctcctgccc
    39541 cggataaaga aaatcatcgt cgctataaac tttgagcagc acaggctgga cccgtccggc
    39601 cttaaccgct gccgcagaaa cttcccgtgc tttgtcgaga gtaaacacgg tgatgatgtc
    39661 ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg atttgtacca gatgcccctg
    39721 atgcgccact ggcaaaccag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac
    39781 aatgccgctg tagcctaatg ccagcaattt ttccgccagc cacggattac gaccaaattg
    39841 cttggtcatc agatacagtt caatgccgta aagccgcgcc gtctcaatga gccgcttacc
    39901 gttttccagt atctgatcca cgtcgatcac ccagctgtcc ggggcgattt tgccctgctg
    39961 ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa
    40021 catcttgttg cctcgtttct gttccttgaa ttaaatattc atttttttaa atatttaggt
    40081 tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt tagcagccat ccataatcca
    40141 taaagattgg ccagaaaata gccttcttca cacgggttaa cttccagagc gaattccttc
    40201 aacaacacct gatgcaattg cacaacggct tgccagtggc tggattgcgc cagttccgcc
    40261 agcaactcgt tatccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcactc
    40321 gccatatgtg tcatcgccat cgttccttgc tcactgcgca ccggcagatg gcactctttt
    40381 tccagtacgt tgacgacctg catcatgcct ttgcagacgt ccttatcaat aacgcctgcc
    40441 tcgcaaagca ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactgggttc
    40501 ctgttcccca ctcaccaccc gttgttgatg ggcaagcagg gcgtgtttat ctacaacggc
    40561 acgcagcaat tgttgcattg ggtaatcatc gactcgcgtc aggaccacgg cttcggtggc
    40621 cagtaaaaat cgcggatcgt ctgttagcgg cgtcgcctct aatcccagca tggtcagttc
    40681 gttttccgcc accacgttcc agatcaccgc atcgacatcg cctttaacaa tgcgttgtaa
    40741 actctcgtga taagagagat cgactcgttc cacatcacta tcgccaaaaa agacatcggt
    40801 catgattttc tgatccgccg aacgactatc gagacctact cgcttaacat ttccagactc
    40861 acctttgcgg catatcagtt gatgttcacc aacatacgta tgcgggccaa gctcgagggc
    40921 gatgcacaaa ttgttttggc tgagataact ttccgccgcc aggcgggaaa ctactgccat
    40981 gtcatagaca ccgttaagta aacactccac gcgaatatcc gcgccgcgca tatgcgcata
    41041 gtaaaaagga atgccatcaa actgggcttt caatccgctc gctaaacctt cgtacaagcg
    41101 ggtatagggc aaaggcatcg cacataccac gttgttgata tccacatgac tcagcaatgc
    41161 tttgttatcc atctcgacaa gataactgcc attgcgccca cggcgttcaa tccgtatcgc
    41221 tccgcttgat tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc
    41281 agttgccagc tcatctatgg ttttcagtcg attaccgcat ttttcaccca acaaataacg
    41341 ggccagtgtc gtgatgacga cgccttcttt tttgataaac gttcgacgca taatatattt
    41401 tcaataaaat gaatatttat atcttcatta aaatgaagat aagtggcgac gacgagatgt
    41461 ggaaacggcg aggcgcttca cattttttcg ggttgttgcg aaaagaggat aaaaaaaccg
    41521 ggtttccccg gtttcagagt gatgacgaag tgatttattc gacaatgcga gcaaagggta
    41581 attcccactg gcgcttgcct gctcccctca ccctaaccct ctccccaaag gggcgagggg
    41641 accgtccgag cgttttgata ttacggcttc gccacaaaac caatcgcttc gtacaccgct
    41701 ttcagcgtac gggaagcgtg tacactggct ttttctgcgc cgtctttcat cacctgttgc
    41761 aggaaggctt catcgttgcg gaaacggtga tagcgttcct gcaattcagt cagcataccg
    41821 gaaacggcat cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt
    41881 tccagttctg ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg
    41941 cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg
    42001 cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg
    42061 ttatcgtcag acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca
    42121 gatttcggaa taaacggctc cggcacctta aagatctcgc catacagcgc gttgaaacgc
    42181 tgggcgatat cgcgactcag ttccaggtgc tgtttctggt cttcacccac cggtaccaga
    42241 ttagtttgat acagcaggat gtccgcagcc atcagcaccg gatagtcaaa cagaccagcg
    42301 ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg actcagttcg
    42361 ccgaagtagg tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac
    42421 tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta
    42481 tccaacgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca
    42541 acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc
    42601 atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta
    42661 gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt
    42721 tatagactga taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc
    42781 gtaggttaag ccaactgatg ggcaacctgc cgcttttgcc gcctgaatat cattgcgtga
    42841 gtcgccgaca aacagcatct gttgtggggc aattcccatc cgctcagcca ccagtaacag
    42901 cgggtccgga tgcggttttt tgttttgcac atcatcgccg ccgataacca cgctaaagta
    42961 tttggcaata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac
    43021 caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa
    43081 caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcagaat
    43141 acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttcccca ttgttttacg
    43201 cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa catctgcgcc
    43261 gttaccaatc caggtaataa cgcgttcttc acctgcgacg ggcaactcca gcgcatacag
    43321 cgccatatct accgcagcag caagaccagg agcactgtcg accagcgtac catcaagatc
    43381 aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc
    43441 gcatttcatc aatgactttt ttatagtctg gctggtcgaa gattgccgaa ccggcgacga
    43501 acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca
    43561 cttccagacg aatgtcaaag ccagactcgt cgatacggcg acgtacttcg cgcagtttat
    43621 ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg gttgacggac atcagcagga
    43681 tcacatccag cttatccatc acgtaatcca gatagctcag aggtgtcgcc gggttaaata
    43741 ccagacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg
    43801 aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc
    43861 gatcgacggg tttcaccatc agatgtacgt cgataggggc ggtaatgccg tagttacgca
    43921 aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt
    43981 caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg
    44041 caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga
    44101 gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac
    44161 ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt
    44221 tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat
    44281 atggcgctca accagacctt cggcgatctc cgccagatgc gcttgttgtt caagcgtaaa
    44341 actgtttgtg tgatacgccg taaagttggc ggtcgcagac agcggtgcat aaggcggatc
    44401 gcaatagacg acggatgcat catctgcgcg ctccatgcta tcggcgtaag actcacaata
    44461 gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg
    44521 ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc
    44581 gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct
    44641 tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa
    44701 cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact
    44761 gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac
    44821 cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat
    44881 atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat
    44941 gccgactaac taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct
    45001 tcaggcagct cccgcaacga taaaccattg cgggagataa ccttgattac ttcagatcgg
    45061 cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctgaacatct gctggcaatg
    45121 tagataccgc ttttttcgcc tcttctttcg aagcgtacac gccagaaacc aggacatacc
    45181 acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg
    45241 cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg
    45301 acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg
    45361 ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg
    45421 ctggcgtaga agtcgcagcc ggtgccttcg tgctcgccac aggggcagct ggttcagtac
    45481 gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc ggttttttcg
    45541 gttcaatcac cgcctgctga cgaactgggc gcgtagtgga cggacgttca gcggtttgcg
    45601 ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt gcgggttcag
    45661 tcggcaacgt ggaattgacc gccacattgt tcagctgttg ctgattttgt ggctgggtca
    45721 gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt gccaccgggg
    45781 tttgcccttg agtcggcgta gaagagatcg gtggcagaga aacatcctgc tgagtatttt
    45841 ccgcagacgt ggttcccggc gctggctgca cgccattcgc ctgatcggtc gcattgccag
    45901 caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagtggtc gagggggctt
    45961 ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg acgcccatca
    46021 tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct
    46081 ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg
    46141 gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt
    46201 caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag
    46261 attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt
    46321 tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct
    46381 gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa
    46441 tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca
    46501 attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata
    46561 tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc
    46621 gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac
    46681 gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac
    46741 cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac
    46801 ccggtttcgc gctcgtcggc agcgacaact tctgccttca gttcacaaca acggcgaata
    46861 cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag
    46921 ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct
    46981 aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga agcaggctgg
    47041 tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg
    47101 gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac accgcgctga
    47161 taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccagc
    47221 gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa agacggtatc gagtacagct
    47281 aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt
    47341 tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgct ggtgaccaac
    47401 atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat
    47461 gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga
    47521 catccacctt aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt
    47581 ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg
    47641 tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt
    47701 cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa
    47761 gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac
    47821 gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg
    47881 tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga
    47941 aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt
    48001 ccatattgag tttttgagct aactggcgcc caatagtgct ttttccggca cccataggcc
    48061 caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta
    48121 atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata
    48181 ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat
    48241 cacccatccc tctgcatatc tggtcgctgc aagcgcactg ccttgctacc accgctctgg
    48301 cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa
    48361 atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta
    48421 tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa cattaccgct
    48481 tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt
    48541 cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc
    48601 aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac
    48661 gtttctccgc tttttacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact
    48721 tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc
    48781 cggatgcgac ctttttgtaa caccgtgggc gtgacctcca tccccaggac ggcctcttta
    48841 aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctcccc
    48901 tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc
    48961 agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc gttgatgcgc
    49021 ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct accaagcgtg
    49081 gtgacttgcc caacgccacc agcgtgttgc gcatcggcca gcgtccattt cacgcctaac
    49141 tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac ctgcccgacc
    49201 ggcagatcca tttgcgctac ccactgttca agcgcgctta acgccgtttt gttatctcgt
    49261 agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc
    49321 tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca gggttatact gcgattttcc
    49381 agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat attgtcattc
    49441 tgccaggcaa tggaatgcac tgagagaatg ttgccttcct gccgcgttat cagtccggcg
    49501 cttttcacta cagtttgtag tgcctgcttc cagggaacat ctgttagatg taacgacacc
    49561 gtaccgctga cgtctggcga cacgaccagg ttcaacttct cctgttcagc cagcgcctgc
    49621 aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc
    49681 tgtacgccgg atatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc
    49741 ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc ccggtaccca gcgttagtac
    49801 gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acatcgtttt gctgcacccg
    49861 tcgccatttc ttttgcccgt cttttattac accgatgatg cgctcgcctc gccctaccat
    49921 cccctgatag cgccactggc taagttcgct aatccggcat agatcttccg gcgatttaaa
    49981 agggtcacgc ataccggtta aaaggcacaa tgcaatacct gccaacaacc agcgtttaac
    50041 cctcatgcgg catctccagt tgtagcgtga acaaaagatc atcaccttcc acgcttaacg
    50101 aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt
    50161 cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga tggatgccag gaaaccagtt
    50221 gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttcc tcgctgaagg
    50281 gagtggtgtc taccaggcga tacagtgcgg cccactgctg gtgatgactt gcccgtagtc
    50341 gaattaatgc gtcgcgctct tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca
    50401 tcagcaacca gaatgcccag caaaactggc ggaggcgggg tgatgtggcg aaccaccagt
    50461 caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc
    50521 tgcgcatcct gctgcgtggc tccccgctga ttgagatgaa aagacgcatc ctggcggagt
    50581 gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtca gccccttgat ctccagcgtt
    50641 ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct
    50701 tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt
    50761 tcgcgtaact gctgttgctc cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt
    50821 tgttgttccg cctgaagcaa aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc
    50881 agtattagcg ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa
    50941 cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc
    51001 agtacgcctt cccaagcgcc agccccagcg cgatggcaaa gtctccacca ggcggcggta
    51061 gcggcggctg acgaacagaa acggcctccc aggggtcaaa tccgccttcg ccacatatcg
    51121 cgacgctgtc gggatcaacg gataacgctg ccgccagctc cttcgcgcta gtcatcccca
    51181 ccgccagttt gcgcccccag ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc
    51241 aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat
    51301 ccggggtgat cgcactaaca tgaacacgca acctttctgc cagcgtaagc agcgttgcca
    51361 gctctttgct ttgcgcggca gtcacgttat aagcggggct gagtgagtct tcgctgtaat
    51421 cgaagcgcag ggagtccgga tccatatcca gctcgcgggc catcgtccct gacagccagg
    51481 ccgtttgctc ccgctcacca agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc
    51541 gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcgcga ctccacggta
    51601 acaacgtttt agccagctgc tgcgcatcaa caatccgccc atctttgata atgtcgtgcg
    51661 ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt tgcgccccgc acgatcgcaa
    51721 ccgctgccac ttcttgctgt tgtaaatgca aaccaatttg ccagatctta aatgtcattg
    51781 tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt
    51841 atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg
    51901 aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg
    51961 atttatggcc tataccgcta catcgagcca caactgccgg atgtggtgac attaaaagat
    52021 gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac
    52081 ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc caccggagat ggtgaaagcc
    52141 tttatcgcga cggaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc
    52201 ttccgtgcgg caagtgtggc gctgttctcc ggccacgcgt cacaaggggc gagtaccatt
    52261 acccagcagc tggcgagaaa tttcttcctc agtccagaac gcacgctgat gcgtaagatt
    52321 aaggaagtct tcctcgcgat tcgcattgaa cagttgctga cgaaagacga aatcctcgag
    52381 ctttatctga acaagattta ccttggttac cgcgcctatg gtgtcggtgc tgcggcacaa
    52441 gtctatttcg gtaaaacggt cgaccaactg acgctgaacg aaatggcggt gatagccggg
    52501 ttgccgaaag cgccttccac ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg
    52561 cgtaacgtcg tactgtcgcg gatgctggat gaagggtata tcacccagca acagttcgat
    52621 cagacacgca ctgaggcgat taacgctaat tatcacgcgc cggagattgc tttctctgcg
    52681 ccgtacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat
    52741 gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcgcagcag
    52801 gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat
    52861 gtgctgtgga aagtgggcga gtcggcgtgg gataacaaca agattaccga tacgctgaag
    52921 gcgctgccga cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcaggga
    52981 gcgacggcga tgctggcgga cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg
    53041 gcgcgtcctt accgttcgga tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt
    53101 ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg atgcatggtg gctggcacaa
    53161 gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaatggtgc cgttatggcg
    53221 ctggtcggtg gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt
    53281 caggtgggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctgacg
    53341 ctggcaagta tgttgaacga tgtgccaatt tctcgctggg atgcaggtgc cggttctgac
    53401 tggcagccga agaactcacc accgcagtat gctggtccaa ttcgcttacg tcaggggctg
    53461 ggtcagtcga aaaacgtggt gatggtacgt gcaatgcggg cgatgggcgt cgactacgct
    53521 gcagaatatc tgcaacgctt cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg
    53581 gcgctgggtt cagcgtcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg
    53641 aacggcggct tcctagtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc
    53701 gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc agtgatttac
    53761 ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc
    53821 cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta
    53881 gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca tcaacactcc gctggcattc
    53941 ctgattaaga gtgctttgaa caccaatatc tttggtgaac caggctggca gggtaccggc
    54001 tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac
    54061 agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt
    54121 ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat
    54181 cagatctcag gttacgaagg cggtgccaag agtgcccagc ctgcatggga cgcttatatg
    54241 aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc cgcctccggg tattgtgacg
    54301 gtgaatatcg atcgcagcac cgggcagtta gctaatggtg gcaacagccg cgaagagtat
    54361 ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg aggtgggaac gaccattatc
    54421 gataatggtg aggcacagga attgttctga ttaaaaaggc gcttcggcgc cttttcagtt
    54481 ggctgacaaa gtgcatttgt ttatgccgga tgcgggtgaa cgcgttatcc ggctaacaaa
    54541 atcgtgaaaa atcaatatat tgcaggaacc ccataagcct gataagcgtt gtgcatcagg
    54601 caaacttcac gcatttacac tcgcccctgc cctttcaacc attcgcgcac gaggaacagc
    54661 gcgctgacat tacgcgcttc gttgaagtca gggtcttcca gcaaatccat catatgcgcc
    54721 agcggccagc gcacctgtgg tagcggctct ggctcatcgc cttccagcga ttccgggtag
    54781 agatcttgcg cgaccacgat attcatttta ctggaaaagt aagacggtgc catgctgagc
    54841 ttcttcaaaa aagtcagatc gttcgcacca aatccaacct cttcttttag ctcgcggtta
    54901 gcggcttcgt agacgctttc acccggatca attaatcctt tcgaaaaacc taattcgtag
    54961 gattcagttc ccactgcgta ttcgcggatc aggatcaggt gatcgtccac aatcggcaca
    55021 atcatcactg cttcccggtt ggttggacgc attcgttcat aaacacgccg cacgccattg
    55081 ctgaactcca gatccacact ctcgacggta aacagtcggg aacgggctac agtttcaaca
    55141 ttcagaatgg tgggtttttg taatgatttg ttcatcgtgg gatctatgct gtgaaatcag
    55201 cggttattgt gcgatatcgg acacgctttc ggcaatgtga attgcatgtt atttacattt
    55261 atgtaactta ataaataatc gtcctcaaat caaattaaaa gtcaataggt tgaaataact
    55321 ccaggaattt gctgatattc cgccttcaga gggtttgcta tgatcagcgg ttactgtgat
    55381 gtgcttaatg atgctcaagt taaactccac gcttgccgat agccaaccgc agaatcatgt
    55441 attgtgtccg gtgcgactga ccacgcctga cagactaagt aagatgggga aagcatgagc
    55501 accattgtga tttttttagc tgctttgctg gcctgctcac tacttgcggg atggctgata
    55561 aaagtgcgat ccagacggcg tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg
    55621 cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt atcttgagag cctgacgcag
    55681 gtattacagg tgcctggccc aacgggagct agcgcggcac cgatctctct ggcgctgaat
    55741 gccgaaagca acaacgtcat gatgctgaca cacgctatca cacgttacgg catctctacc
    55801 gacgatccga ataaatggcg ttactacctc gattcggtag aagtccacct gccccccttc
    55861 tgggaacagt acatcaacga tgagaatacc gttgaactga ttcataccga ttcgctgccg
    55921 ttggttattt cactcaacgg tcatacgctg caggagtaca tgcaggaaac gcgcggctat
    55981 gccttgcaac ctgttccgtc cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc
    56041 gagctactca atattcgcaa agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg
    56101 ctgcgtgaag cgttgctgat cgtcgcctcc ttcctgatgt tctttttctg cctgattacc
    56161 ccggatgtat ttgttccgtg gctggcaggc ggcgcattac tgctgctggg cgcaggtctg
    56221 tgggggctat tcgcgccccc ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt
    56281 acaccccgtc gttggggatt gtttggcgaa aacgatcagg aacagatcaa caatatttca
    56341 ctcggtatta tcgacctggt ctatcccgca cactggcagc catacattgc tcaggatctc
    56401 ggtcaacaaa ccgatattga tatctatcta gaccgccacg tagtgcgtca ggggcgatat
    56461 ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc actggctgcg cagtacgatt
    56521 atcgctgcgg gctcgctgct ggtgctgttt atgctgttat tctggatccc gctggatatg
    56581 ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga ccattgaagc caccagcgta
    56641 aaacaactgg ctgacgctgg cgtgcgggta ggcgatacat tgcgtattag cggtacggga
    56701 atgtgtaata ttcgcacttc cggaacctgg agcgcgaaaa ccaattcacc ttttttacca
    56761 tttgactgct cgcagatcat ctggaatgac gcccgctcat taccattacc agaatctgaa
    56821 ctggtcaaca aagcgacggc attgactgaa gcagttaatc gccagctgca ccctaaaccg
    56881 gaagatgaat ctcgcgtcag tgcctcatta cgttcagcga ttcaaaaatc cggcatggta
    56941 ttgcttgatg attttggcga cattgtactg aagacagcgg atttatgttc tgccaaagat
    57001 gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca acagtaaaga ctgggacgcg
    57061 ctggtaaaac gcgccaacgc tgggaagctc gatggcgtga atgtgttatt acgcccggtg
    57121 agtgcggaat cgctggataa cctggtggca acctccaccg cgccgtttat cacgcatgaa
    57181 acggcgcgag cggcacaatc actaaacagt ccggcccccg gcggattcct gattgtcagc
    57241 gacgaaggca gcgattttgt tgatcagccc tggccttcgg catcacttta cgactacccg
    57301 ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa tgctgatgca tacgccgttt
    57361 aacgccgaag gtatcgtcac aaaaatcttc actgacgcca atggtacgca gcatattggc
    57421 cttcatccga tcccggatcg ttccggcctg tggcgctatc tcagcaccac attgctgcta
    57481 ctgacgatgc tgggtagcgc catttacaat ggcgtacagg cctggcgtcg ttaccagcgt
    57541 catcgcactc gcatgatgga gattcaggcc tattatgaaa gctgcctgaa cccgcaactg
    57601 atcacccctt cagaaagcct tatcgaataa cacgtttgcg cggcaggtta tgctaccctg
    57661 tcgcgcaaat tgcttcactc tggagatttc cctcatgcat atcaacattg cctggcagga
    57721 cgtagatacc gttctgctgg atatggacgg cacgttgctc gacctcgcct tcgataacta
    57781 tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa aacggggtta cgccacagga
    57841 agcgatggaa tatatgcgcc agcaatatca cgacgtacag catacgctaa actggtactg
    57901 tcttgattac tggagtgagc aactgggtct ggatatctgt gcgatgacca ccgagatggg
    57961 accgcgcgcc gtactgcgtg aagataccat tccgtttctt gaggcactga aagccagcgg
    58021 taagcagcga attttgctca ccaatgctca tccgcacaac ctggcggtaa aacttgagca
    58081 tacgggtctg gacgcacacc ttgatttatt actttccacc cacacatttg gttatccgaa
    58141 agaggatcag cggttatggc atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac
    58201 gctgtttatt gatgacagcg aagcgattct cgatgctgcc gcgcaatttg gtattcgtta
    58261 ctgcctcggc gtgactaatc ctgattccgg gattgccgag aaacagtatc aacgccatcc
    58321 gtcactgaat gactaccgcc gcctgatccc ctcgctaatg tgaaggagac gccatgaaag
    58381 agaaacctgc tgttgaggtt cgactggata aatggctatg ggctgcccgt ttttataaaa
    58441 cccgcgcgct ggcccgtgaa atgattaaag gcggtaaggt gcattacaac gggcagcgca
    58501 gcaagccgag caaaatcgtc gagctgaatg ccacgctcac tctgcgccag ggaaatgacg
    58561 aacgcacggt gattgtaaag gcgattactg aacagcgtcg ccccgccagc gaggcagcct
    58621 tgctgtatga agagactgcg gaaagtgtag agaaacgcga aaaaatggcg atggcacgta
    58681 aacttaatgc cttaaccatg ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg
    58741 acctgttacg atttaaacac ggcgacagtg aataactgtc acctgcaaga gagatgatta
    58801 tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac
    58861 tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa ccacgattat ccgcagcccg
    58921 ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct gttaaccgct acgctgaagt
    58981 ttgatggtga tatcaccgta cagctgcagg gcgacggtcc gatgaatctg gcggttatta
    59041 acggtaacaa taaccagcaa atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa
    59101 atgccgacct gaaaacgctg gtcggcaatg gttacgtggt gatcaccatt accccgagcg
    59161 aaggcgaacg ctatcagggc gtagttggtc tggaaggtga taccctggcg gcctgcctag
    59221 aagattactt tatgcgttct gaacagctgc cgacgcgcct gtttattcgc accggcgacg
    59281 tagacggcaa accggctgca ggcggtatgt tgttgcaggt aatgcctgcg caaaataccc
    59341 agcaggacga ctttgaccac ctggcgacgc taaccgaaac catcaaaacc gaagaactgc
    59401 tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca cgaagaagag gtgacggttt
    59461 acgatccgca ggatgtggag ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc
    59521 tgaaaacgct gcctgatgaa gaagttgata gcatcctggc ggaagatggc gaaattgaca
    59581 tgcattgtga ttactgcggt aaccactatc tgttcaatgc gatggatatt gctgaaatcc
    59641 gcaacaacgc gtctccggca gatccgcaag ttcattaatg agtatgtccg gcagagaggg
    59701 ctctgccgga ttttacgact taatggcctg atgtgctgcg cttaccagcg cctatgaaat
    59761 aaatcgcaaa ttattttggt cagatacagc gtcgtatccg ataaaagcgt tgtcacttac
    59821 ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg
    59881 tccccagttt tccatccttc gcagctgtga gtaatttttc tttcgttatt tcgttcggaa
    59941 gtaaactcat atcaagcgcg tcccattctt ttctgtctga gtcaaagcca tagacaatga
    60001 ctctgtcatc attaaaagca aacaggatcc gctccgcctg accatcacta ttcagatctt
    60061 gctcgaccag tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgatcctgga
    60121 ttaaggccga ccagaatgtc gcatccggtt taacagaacc aggggcaatt aacacatttt
    60181 cggctaatac tttttccgat acctgttcct gaagatgttg ctctccatct aacgccatca
    60241 gcagatcccg cgcgcgtttc gggtctttca taaacccggc atcgcttttc agcgactcaa
    60301 gcgccgcacg tccatagcga ccgctctgtt cgagcatgta gatagttacc tggtctggcg
    60361 tgtttttgcc gctctgataa cgcgccatat gactgttcac gctaatacgc atactgtcca
    60421 gcaccggcga attaagaagc accagtatca ccaacaccaa taacgaaacc gcaaggttca
    60481 ctttgccctg aagaacaagg ggattttgcc cattacgcca gacgatactg acaaaatacc
    60541 ccagcgacca caccagtaac accagctccg ccagcgcgcc ctgcaagcgg tcgacagtcc
    60601 agccgtactg agcgacccgc agccataacc cccaggtggc gacgaacaca tacagcggag
    60661 caaccagcaa agcggtttta atcaggcaac gcaacggccc cgtccaggga agtgacgctt
    60721 tttgcggatc gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca
    60781 acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc cgtaaacggc agggtgatga
    60841 taaacatcag ggttagcaat gatactaacg gcagcaaccc cgtggcgatt agcgtgaaca
    60901 acttttgaat agagtcgatt aaacgtgact gtgtacgcgc gaggatcacc gccagtgcgg
    60961 taaccagacc aaatgtaaga tacataaacc agtcggttgc aaaaaagagc gtattgaaaa
    61021 acgtgatgcc aacaagttta aacaactcac cccacagtaa aagcaccagc cacgtcaggc
    61081 cattggagag aaaaatcacc agtaatataa gtacgttatg ccatactgac tggtaaaagt
    61141 agctatagcg gctactgcca ttgcggatac gaaggctttg ttgtatccac ggtagcaaca
    61201 acattgccat caacagcaga tagcagccaa aatcccaaat cgccttttca gctctccagg
    61261 ggttcatgcc atcagtttgc cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca
    61321 ccagcgccag ccatccccaa aggcgcttct gtttaaaaga gatcacggag aagagtaaaa
    61381 cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat ccaactgtga ttttttccgg
    61441 caagccaggc gataagcagg tagcaaacca gcccctgaag taatccggta gcaatcatcc
    61501 cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt ggttatccag aatcaaaagg
    61561 tgggttaatt atcgcatccg ggcagtagta ttttgctttt ttcagaaaat aatcaaaaaa
    61621 agttagcgtg gtgaatcgat actttaccgg ttgaatttgc atcaatttca ttcaggaatg
    61681 cgattccact cacaatattc ccgccatata aaccaagatt taaccttttg agaacatttt
    61741 ccacacctaa aatgctattt ctgcgataat agcaaccgtt tcgtgacagg aatcacggag
    61801 ttttttgtca aatatgaatt tctccagata cgtaaatcta tgagccttgt cgcggttaac
    61861 acccccaaaa agactttact attcaggcaa tacatattgg ctaaggagca gtgaaatgcg
    61921 cgttaacaat ggtttgaccc cgcaagaact cgaggcttat ggtatcagtg acgtacatga
    61981 tatcgtttac aacccaagct acgacctgct gtatcaggaa gagctcgatc cgagcctgac
    62041 aggttatgag cgcggggtgt taactaatct gggtgccgtt gccgtcgata ccgggatctt
    62101 caccggtcgt tcaccaaaag ataagtatat cgtccgtgac gataccactc gcgatacttt
    62161 ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa cctctctctc cggaaacctg
    62221 gcagcatctg aaaggcctgg tgaccaggca gctttccggc aaacgtctgt tcgttgtcga
    62281 cgctttctgt ggtgcgaacc cggatactcg tctttccgtc cgtttcatca ccgaagtggc
    62341 ctggcaggcg cattttgtca aaaacatgtt tattcgcccg agcgatgaag agctggcagg
    62401 tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga
    62461 acagggtctg aactccgaaa acttcgtggc gtttaacctg accgagcgca tgcagctgat
    62521 tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg ttctcgatga tgaactacct
    62581 gctgccgctg aaaggtatcg cttctatgca ctgctccgcc aacgttggcg agaaaggcga
    62641 tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc accctttcca ccgacccgaa
    62701 acgccgcctg attggcgatg acgaacacgg ctgggacgat gacggcgtgt ttaatttcga
    62761 aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa
    62821 cgctatccgt cgtgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg gcactatcga
    62881 ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct tatccaatct atcacatcga
    62941 taacatcgtt aagccggttt ccaaagcggg ccacgcgact aaggttatct tcctgactgc
    63001 tgatgctttc ggcgtgttgc cgccggtttc tcgcctgact gccgatcaaa cccagtatca
    63061 cttcctctct ggcttcaccg ccaaactggc cggtactgag cgtggcatca ccgaaccgac
    63121 gccaaccttc tccgcttgct tcggcgcggc gttcctgtcg ctgcacccga ctcagtacgc
    63181 agaagtgctg gtgaaacgta tgcaggcggc gggcgcgcag gcttatctgg ttaacactgg
    63241 ctggaacggc actggcaaac gtatctcgat taaagatacc cgcgccatta tcgacgccat
    63301 cctcaacggt tcactggata atgcagaaac cttcactctg ccgatgttta acctggcgat
    63361 cccaaccgaa ctgccgggcg tagacacgaa gattctcgat ccgcgtaaca cctacgcttc
    63421 tccggaacag tggcaggaaa aagccgaaac cctggcgaaa ctgtttatcg acaacttcga
    63481 taaatacacc gacacccctg cgggtgccgc gctggtagcg gctggtccga aactgtaatg
    63541 atttgaagct ggagaatatc tatccagtat cttatagaaa gcaaaacggg aggcaccttc
    63601 gcctcccgtt tatttaccct tcttttgtcg tgccctgcgc ccgcgttacc ggcactggca
    63661 gccaggcgcg aatggaaagc ccgccccgct cgctggtgcc aagctccagc atcccgttat
    63721 ggttatccac gatacgctgc acaattgcca gccctaatcc cgtgccgcta atggtgcgcg
    63781 cactgtcgcc gcggacaaac ggctggaaca ggtgcttacg ttgttccggc gcaattcccg
    63841 gaccgtcatc ttccacctgg aaccaggcgc gattcggctc cgttccgctg ctgactttga
    63901 tccagccatt gccataacgg gcggcgttga ccaccatatt cgccaccgcg cgtttgatcg
    63961 acagcgggtg cattttcact tcaatgctgc cggggtaaag cgcggtttca atttcccgct
    64021 catagccact ttcggcagca atcacctcac cgagcactgc attaagatcc gccatttcca
    64081 tcggcatctc ctgcccggtg cgcaggtagt cgataaactg ctcaatgatg gcgttgcact
    64141 cttcgatatc tttattgatc gattctgcca gatagccatc ctgctcgctc atcatctcag
    64201 tcgccaggcg aatacgcgtc agcggcgtgc gcaagtcgtg acttaccccc gccatcagca
    64261 gcgtgcggtc atccgccagt tgcttaacac cagccgccat atggttaaag gcacgggtaa
    64321 cggaacgcac ctccgaagcg ccatattcac gcagcggcgg cggaataatc cctttaccaa
    64381 cctgcaaggc tgcgtgttcg agatcgacca acggtcggtt ctggatacga ataaacagcc
    64441 acgccccgcc tatcgccaat agcataatcg ccagcgtata gcggaacagc ggagagaaat
    64501 cgccctgatg aatttcggtc agcggcacgc gtacccagat attgggcgac agccaggttt
    64561 tcagccagac gacaggcgaa cttttgttga cctcaacgcg cacttccgtc gggccgccca
    64621 gttgctgtgc catctgatgg cttaagaatt catagtgttg cgcccaacgc agacctgcct
    64681 cttcggcagc ctcgttggag tagagagaga tccccagctc acggtagatc tcccgacgga
    64741 aagcgggagg cacaaccaac tgcgtgccgt cctccagttg cagtttatcg gtcatcaaca
    64801 tacgcacttc gtacgcgagg actttattaa actgctggag gctcggcaaa atcgcgaagt
    64861 tcagcaccac cagataagtc gtcaccaggc tggcgaacag caaggtgacg atgagcaata
    64921 acgtacgggc aaatgaactt cgtggcgaga agcgcaatcg cctcatgctt tagagccgtc
    64981 cggtacaaag acgtagccca ggccccaaac ggtctgaatg taacgcggat gcgctggatc
    65041 ttcttctacc atgcggcgca gacgcgaaat ctgcacgtcg atggagcgtt ccattgcgga
    65101 atattcacga ccacgggcaa ggttcatcag cttatcgcgg gagagcggct cacgcggatg
    65161 gctgaccagt gccttcagta ccgcaaactc accgctggtg agcggcatcg gctcatcttc
    65221 gcggaacatt tcgcgcgtac cgaggttaag tttgaactta ccgaaagcaa ttaccgcctc
    65281 ttcctgtgac ggtgcgcctg gcagttcgtt cgcctgacga cgcagcaccg cacgaatacg
    65341 ggccagcagt tcacgcgggt taaacggttt tggaatgtag tcgtcagcgc caatctccag
    65401 gcctacgata cggtccactt cttccccttt cgccgtcacc ataatgatcg gcatcgggtt
    65461 gctctgacta cgaagacgtc ggcaaatcga caagccatct tcaccaggta acattaaatc
    65521 cagtaccata agatggaaag attcacgagt cagcaggcga tccatctgtt ctgcattagc
    65581 gacgcttcga acctggaagc cttgttcggt gagataacgt tccagcagcg cacgcaggcg
    65641 catgtcgtca tcgaccacca gaatcttgta gttctcttgc attgtttgta ctcccaaagg
    65701 ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca cgttcgtcac cagctaaatc
    65761 tggtatgaat ttcagcctaa attgttacaa agcatattaa acagcagctt aagtatacaa
    65821 tttattcggc gaaacattat tgattctgtt gatatgatca cgttataccc aatgtgcgca
    65881 ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc cctggttacc cgggaagggt
    65941 atgaaaaact caaacaagag cttaattatc tctggcgtga agaacgcccg gaggtcacaa
    66001 aaaaggtgac ctgggccgca agtctgggcg accgcagcga aaatgctgac tatcagtata
    66061 ataaaaagcg tctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa tgcctggaaa
    66121 atctcaaaat tgtcgattac tcccctcagc aggaaggcaa agtctttttt ggcgcgtggg
    66181 tggagattga aaacgacgat ggcgtgactc accgtttccg tattgtcggc tacgatgaaa
    66241 tttttggccg taaagattac atctctatcg attccccgat ggcccgcgca ttgctgaaaa
    66301 aagaagtcgg cgatctggcg gtggtgaata cccctgccgg ggaagcgaac tggtatgtta
    66361 atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac ctcctgccga tggctggcat
    66421 ttttgccagc cagtccgtat aactatcccc tgatttttga tccgaaaaga tgaactcaaa
    66481 ccatgatgaa tgattcgttc tgccgcatta ttgcgggtga aattcaggcg cgcccggaac
    66541 aggttgacgc tgccgttcgc ctgcttgatg aagggaatac cgtgccgttt atcgcacgtt
    66601 atcgtaagga aatcaccggc ggtctggatg acacgcagct gcgtaatctg gaaacgcgtc
    66661 tgagctatct gcgcgagctg gaagagagac gtcaggcgat cctcaaatcc atttccgagc
    66721 aaggcaaact caccgatgat ctggcgaagg ccatcaacgc caccctaagc aaaaccgaac
    66781 tcgaagacct ctacctgccc tacaaaccta aacgccgcac ccgcgggcaa atcgccattg
    66841 aagcagggct tgagccgttg gctgacctgc tgtggagcga tccgtcacac acgccagaag
    66901 tcgccgctgc acaatatgtt gatgccgata aaggcgtggc agataccaaa gccgcgctgg
    66961 atggcgcgcg ctatatcctg atggaacggt ttgccgaaga tgccgcgctg ctggcgaaag
    67021 tgcgtgatta tctgtggaag aacgcgcatt tggtttctac ggtggtgagc ggtaaagaag
    67081 aggaaggggc gaaattccgc gactatttcg atcatcacga accgttgtcc acggtgcctt
    67141 ctcaccgcgc gctggcgatg ttccgtgggc gtaacgaagg cgtactccag ctttcgctga
    67201 atgccgatcc gcagttcgat gagccgccca aagagagcta ttgcgagcaa atcatcatgg
    67261 atcaccttgg cctgcgcctg aacaatgccc cggcggatag ctggcgcaaa agcgtagtga
    67321 gctggacctg gcgcatcaag gtgctgatgc atctggaaac cgaactgatg ggtaccgtgc
    67381 gcgaacgcgc ggaagatgaa gcaatcaacg tctttgcccg taacctgcac gatctgctga
    67441 tggcggcccc tgccggactg cgtgcaacga tgggcctcga tccgggtctg cgtaccgggg
    67501 taaaagtggc ggtggtcgat gccactggca aactggtggc gaccgatacc atttacccgc
    67561 acaccggaca agccgcaaaa gcagcgatga ccgttgctgc cttgtgtgaa aaacataacg
    67621 ttgaactggt ggcgatcggc aacggtacag cttcccgcga aactgaacgt ttctatctcg
    67681 acgtgcagaa gcagttcccg aaagtgaccg cacagaaagt gatcgtcagc gaagctggcg
    67741 cgtcggttta ctcagcttcc gagctggcag cgcaggagtt cccggatctc gacgtttcgc
    67801 tgcgtggtgc ggtatctatc gcccgccgtt tgcaggatcc gctggcggag ctggtgaaaa
    67861 tcgatccgaa atctatcggc gtaggtcagt atcagcatga cgtcagccag acgcaactgg
    67921 cccgcaaact ggatgcagta gtagaagact gcgtaaacgg cgttggcgtc gatctcaaca
    67981 ccgcttctgt tccgctatta acccgcgtgg cgggcctgac gcgcatgatg gcgcaaaaca
    68041 tcgtggcctg gcgcgatgag aacggccagt tccagaaccg tcagcaactg ctgaaagtca
    68101 gccgtctggg gccgaaagcc ttcgagcagt gcgcgggctt cctgcgcatt aaccacggtg
    68161 ataacccgct ggacgcctct accgttcatc cggaagccta tccggtggtg gaacgcattc
    68221 tggcagcaac acagcaggca ctgaaagatc tgatgggtaa cagcagcgaa ctgcgtaacc
    68281 tgaaagcgtc tgactttact gatgaaaaat tcggtgtgcc gacagtaact gacatcatca
    68341 aagagctgga aaaaccgggt cgcgatccgc gtccggaatt taaaaccgct cagtttgccg
    68401 atggcgtcga gacaatgaac gacctgcaac cgggtatgat cctcgaaggc gcagtgacca
    68461 acgtcaccaa ctttggcgcg tttgtcgata ttggcgtgca tcaggacggc ctggttcaca
    68521 tctcttcatt gtcgaacaag tttgtggaag atccgcatac cgtggtgaaa gcgggcgaca
    68581 ttgtgaaggt gaaagtgctg gaagtggatc ttcagcgtaa acgtatcgcc ctgactatgc
    68641 gtctggacga gcagcctggc gaaaccaact ctcgtcgcgg cggcggtaat gaacgcccgc
    68701 aaaacaaccg cccggcagcc aaaccacgcg gtcgtgaagc gcagcctgcc ggtaatagcg
    68761 cgatgatgga tgcgctggcg gcagcgatgg gtaaaaaacg ttaaacgccc ggactggccc
    68821 ctaaacctgc gcgataaggc gttcacgccg catctggcaa cgaacgcttt gcctgacata
    68881 aaagtgccgg agaatatctc cggcactttt attccacagc caaactcaca atatactccg
    68941 ttaatattta tcacttcatt aacaactgaa accttaatta aacattagcc agtccgggta
    69001 actcactatt cgaattatat tttcgctgcg atataacctt gagccacatc aacattgagt
    69061 cagattatta ttcaaaccaa cattcgtaca cattttaagt attgctgata gaaaccattc
    69121 tcattatcat tgtgttgttg attatttaat ctctcctttg ttggcaaatc atctggtctc
    69181 atgtcgcggt caaacgcccc atgaggtagt tatccagtta atgagaaaca agtaggcacc
    69241 tatgcaatac actccagata ctgcgtggaa aatcactggc ttttcccgtg aaatcagccc
    69301 ggcatatcgc caaaaactgc tttctctagg catgttacct ggctcctctt ttgatgtggt
    69361 gcgcgtcgct ccactcggcg accccattca tatcgaaacc cgtcgtgtga gcctggtatt
    69421 acgcaaaaaa gatctggcct tattagaagt ggaagcggtt tcctgttaat acggtgataa
    69481 caacaatgaa aaaattaacc attggcttaa ttggtaatcc aaattctggc aagacaacct
    69541 tatttaacca gctcactggc gcacgtcagc gtgtaggtaa ctgggctggc gttaccgtcg
    69601 aacgtaaaga agggcaattc tccaccaccg atcatcaggt cacgctggtg gacctgcccg
    69661 gcacctattc tctgaccacc atctcatcgc agacctcgct cgatgagcaa atcgcctgtc
    69721 actacatttt gagtggcgac gccgacctgc tgattaacgt ggtggatgcg tctaaccttg
    69781 agcgtaacct gtacctgacg ctgcaactgc tggaactcgg cattccctgc atcgtggcgc
    69841 tgaacatgct cgacattgcc gagaagcaaa atattcgtat tgaaattgat gctctatcgg
    69901 cacgtcttgg ctgcccggtg atcccgctgg tttcaacccg tggtcgcggt attgaagcgc
    69961 tcaagctggc gattgatcgc tataaagcta acgagaatgt ggaactggtg cattacgcac
    70021 agccgctact caacgaagca gattcactgg caaaagtgat gccttccgac atcccgctga
    70081 aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga tatctacagc cgcgcctacg
    70141 ccggtgaagc gtcgcagcat ctggatgcca ccctcgcccg tctgcgtaat gaaatggatg
    70201 atccggcgct gcacattgcc gatgcgcgtt accagtgcat tgctgccatc tgtgatgtgg
    70261 taagcaacac cctgacggca gaacccagcc gtttcaccac tgcggtagat aaaatcgtgc
    70321 tcaaccgttt cctcggtctg ccgattttcc tctttgtgat gtacctgatg ttcctgctgg
    70381 ctatcaacat cggcggggcg ttacagccgc tgtttgacgt cggctccgtg gcgctatttg
    70441 tgcatggtat tcaatggatt ggctacacgc tccacttccc ggactggctg actatcttcc
    70501 tcgcccaggg catgggtggc ggtattaaca ccgtgctgcc actggtgccg cagatcggca
    70561 tgatgtacct gttcctctcc ttccttgagg actccggcta tatggcgcgt gcagcgtttg
    70621 tgatggaccg cctgatgcag gcgctgggct tgccggggaa atcctttgtg ccgctgatcg
    70681 tcggtttcgg ttgtaacgta ccgtcggtaa tgggcgcacg tacgcttgat gcaccgcgtg
    70741 aacgtctgat gaccatcatg atggcaccgt ttatgtcctg cggcgcgcgt ctggctatct
    70801 tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc cctggcggtt ttctcgctgt
    70861 atatgctcgg tatcgtgatg gcggtgctga ctggcctgat gctcaagtac accatcatgc
    70921 gcggtgaagc aacgccgttt gtcatggagc tgccggtata ccatgtacca cacgttaaaa
    70981 gcctgattat ccagacctgg cagcgtctga agggcttcgt tctgcgtgct ggtaaagtga
    71041 ttatcatcgt cagcattttc ctgagcgctt tcaacagctt ctcgttgagc gggaaaatcg
    71101 tcgataacat caacgactcg gcgctggctt ccgtcagccg ggtgataacc cctgtcttca
    71161 agccgattgg cgtacatgaa gataactggc aggcaacggt tggcctgttt acgggcgcaa
    71221 tggcaaaaga agtggtggtg ggtacgctca acaccctcta caccgcagaa aatattcagg
    71281 acgaagagtt caatccggcg gaattcaacc tcggtgaaga gctgttcagt gcggtagatg
    71341 aaacctggca gagcctgaaa gacaccttca gccttagcgt actgatgaac cccattgaag
    71401 ctagcaaagg cgacggtgaa atgggtaccg gggcgatggg cgtgatggat cagaaattcg
    71461 gtagcgcagc tgccgcatac agctacctga ttttcgtcct gctgtatgta ccatgtatct
    71521 cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg gatgggcttc tccatcctgt
    71581 gggggctgaa tatcgcttac tcactggcaa cattgttcta tcaggtcgcc agctacagtc
    71641 agcacccaac ttacagcctg gtgtgcattc tggcggtaat cctgtttaac atcgtagtta
    71701 tcggtctgct gcgccgcgcg cgtagccggg tggatatcga actgctggca acccgcaagt
    71761 cggtaagcag ttgctgcgca gccagcacca ccggtgattg ccattaatgg cttcacttat
    71821 tcaggtacgt gatttgctgg cgttacgggg ccgtatggaa gcgacccaga taagccagac
    71881 attgaacact ccgcagccaa tgattaacgc catgctgcaa cagctggaaa gcatgggcaa
    71941 agccgtgcgg attcaggaag aacctgacgg ctgcctctct ggcagttgta aaagctgccc
    72001 ggaaggcaaa gcctgtctgc gcgagtggtg ggcgctgcgt taaccatact ccatcgcctg
    72061 ttttgaaaag cccggtatgc gtttgcatcc gggctttttt gcgtgcggct ttccataaaa
    72121 atgcaattct tgcagcgcaa cgtaagttcc tctgaaagcg tctcgcaggg atgaaaactc
    72181 gctaatgcac agatgtggag tggcgcgtag agtcgcggca ttcaaacaac aggtgaagga
    72241 acgccatgag taaaaagcag agttccaccc cacatgatgc gctgttcaaa ctctttttac
    72301 gccaaccgga gacggcacgc gattttcttg cgtttcattt accggcaccc attcacgcgc
    72361 tctgtgatat gaaaaccctc aagctggagt cgagcagctt tattgatgac gatctgcgtg
    72421 aaagctattc cgatgtgctg tggtcggtga aaacggaaca aggaccagga tacatctatt
    72481 gtctgattga acatcaaagc acctcaaaca aactgatcgc atttcgcatg atgcgttacg
    72541 ctattgccgc aatgcaaaat caccttgatg ctggatacaa aacgttgccg atggtggtgc
    72601 cattgttgtt ttaccacggt attgaaagcc cctatcccta ttcgctgtgt tggctggatt
    72661 gtttcgccga tcccaaactg gcaaggcagc tttatgcctc cgcatttccg ctgattgatg
    72721 tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg tatggcgctg ctggagttaa
    72781 ttcaaaaaca tattcgtcaa cgcgatctga tggggctggt agagcaaatg gcctgcttat
    72841 taagtagtgg atacgctaat gacagacaaa tcaaagggct gtttaattac atactgcaaa
    72901 ctggcgacgc tgtacgtttt aacgatttta tcgacggcgt tgccgaacgt tcaccgaaac
    72961 acaaggagag tttaatgact attgcggaaa gattgcggca ggagggggaa caatccaaag
    73021 ccctgcatat agccaaaata atgcttgaat ccggagtccc tcttgcagac atcatgcgat
    73081 ttaccgggct gtcagaagaa gagttggctg cggcgagtca gtagatactc taccctgctt
    73141 aagtgctact ttgtgctatc ttgcatgtaa cacaaagtag cacttaggga actgatatgc
    73201 agacagtgaa attacggcaa caaggaggag ccatgatcgt tactatccca cgagatcttg
    73261 ccatcgatct tggctggtca ccgggcacag agcttaccgt ggagaaaaaa ggcgatagcg
    73321 ttaatttgcg cgccacagag cacaaaccgc gtgggcgtct taccgtcgcg cagttgttga
    73381 gccaaatcga tgaaagcgaa attacggagc taaatcagtc cacagaagga tgggctgaag
    73441 ggaaaaaagg taatgaagca tggtaaaggc acggacgcca catcgtggtg agatctggta
    73501 ttttaaccct gatccggttg ccgggcatga acttcagggg ccacattatt gcattgtggt
    73561 aacggacaaa aaactcaaca atgttttaaa agttgctatg tgctgcccga tttcaacagg
    73621 ggcaaatgca gcacgttcca caggggtgac ggtgaacgtc ctcccctgtg atacgcaaac
    73681 cggtaacctg catggcgttg tactttgtca ccagctaaaa gccgtcgatc ttattgcccg
    73741 tggcgctaaa tttcataccg ttgccgatga aaaattgatt agtgaagtta tcagtaaact
    73801 ggtgaattta atcgacccac aataattagc tctcacttac caccaacacc ctctgcttca
    73861 acgccaccag caggtgacaa aactcgaccg gatgcgaaat aaacggcgca tgggccgctt
    73921 tggcgaagat atatgattcg ctgtgaggcc aaagtttatc cagcatcggc accactttgc
    73981 gcggcaccag accgtcgaga tagccataca atcgcaaaaa cggcatggac acgttttgca
    74041 gcggctgacg gagatcgacc gttttcagga tttccagccc gccattaagc acgtcaacct
    74101 ccggcatcgg taacgccaga acggttttct tcaacgcccg cgcatcctgg cgcgccgttt
    74161 cagtccccat ggtttgtaac gccaggaacc gctccactgt acgctgaaaa tcatcactga
    74221 gttgctgctg aaatcccgcc agcacgtccg gttttatccc cggccactcg tcacgagcac
    74281 taaaataagg tgacgacgcc acggtgacca gcgcctgaac acgctcggga tgggttaacg
    74341 caatctggct tgccaccagc ccgcccagac tccagcctaa ccaaatggct ttatcaggtg
    74401 cctgttgcag cacggcttcg gccatatcag caagtgacag cgcaccaaat ccccggctac
    74461 gcccgaagcc gggcaggtca acaaggtgca gcgtaaaatg cgagctaagt tcctcgtcaa
    74521 tgcaacgcca cacttcggca ttcagtcccc atccgtgcag cagcacaaga tgaacattcc
    74581 cctgaccttt ggtctgccac cagatgttat tcatccgcta ttgttctctt ttgacttaca
    74641 aggatgaaca tatgctaaca gtaccgggat tatgctggct atgccgaatg ccactggcgt
    74701 taggtcattg ggggatttgt tcggtctgtt cacgcgccac ccgcacagat aaaacgttat
    74761 gcccacaatg tggattaccc gccacacact cccatcttcc ctgcggtcgc tgcctgcaaa
    74821 aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta tgcgccgccg ttaagtccgc
    74881 ttatccacca gcttaaattt tcccggcgca gtgaaatcgc cagcgccctg tcacgtctgt
    74941 tactattgga agtcttacac gctcgtcgca ccaccgggtt gcaattgccg gatcgcatca
    75001 tcagcgttcc gttatggcag cggcgtcact ggcgtcgggg atttaatcag agcgatttgc
    75061 tgtgtcagcc gttatcacgc tggttgcact gccaatggga tagcgaagcc gtcacacgta
    75121 cacgggccac tgcgacccag cattttctca gtgcccggct gcgcaagcgc aacctgaaaa
    75181 atgcctttcg tcttgaattg cccgtgcaag gtcgccatat ggtgattgtg gatgatgtcg
    75241 ttaccaccgg aagtaccgtc gcagagattg cgcagttgct tttacgcaat ggtgcggcga
    75301 ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct cgatgatggg cgtattataa
    75361 ccaactaaaa tagtcaacta ttaggccatt actatgatcc gtatttccga tgctgcacaa
    75421 gcgcactttg ccaaactgct ggcaaatcag gaagaaggga cacaaatccg cgtatttgtg
    75481 attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt attgtccgcc ggatgccgtg
    75541 gaagccaccg acactgccct gaaatttgac ctgctgaccg cgtatgttga tgagttaagc
    75601 gcaccatacc tggaagatgc agagatcgac tttgttaccg accagttggg ttctcagtta
    75661 acgctgaaag ccccgaacgc caaaatgcgt aaagtggcag acgatgcacc gctgatggag
    75721 cgcgtggagt atatgctgca gtcgcagatc aacccacagc ttgctggtca cggtggtcgc
    75781 gtttcgctga tggaaatcac cgaagacggt tacgccattc tgcaatttgg cggcggctgt
    75841 aacggttgtt ccatggtcga tgtgacgctg aaagaaggga tcgagaagca gctgctgaac
    75901 gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac accagcgcgg cgaacactcc
    75961 tactactaag ttatcccctc ttttgtagat tgcccgatgc gacgctaaag cgtcatttcg
    76021 ggcctacgtt gaacatgtgc cagacgttgg cacagcattg ctttggcaaa tccctccctt
    76081 cgtttccccg cgataatatg accaacctct cataatttaa atttaccccg ctctggtgat
    76141 tctcaaacgc cagatgttac ccgtatcatt cacatgggta ccaaacatac tcctgacatc
    76201 tgactacaat aattagtttt agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg
    76261 attgggataa ttagagtttg tcgtcagaaa attgacatta cccataacaa atgaaaggcc
    76321 aggtaaatca tgccattagt cattgttgct atcggtgtaa tcttgttgtt gctcctgatg
    76381 atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta
    76441 atgcaaggaa tgccgctgga taaagttatt ggctccatca aagccggtgt cggcgggacg
    76501 ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc tggcaaactg cggtggcgca
    76561 caacgtatcg ccaccacgct gattgccaaa tttggtaaaa agcacatcca gtgggcggtg
    76621 gtgttaaccg gttttaccgt tggttttgcc ctgttctatg aagtgggctt cgtgctgatg
    76681 ctgccgctgg tgtttaccat cgcggcttcc gcgaatattc cattgctgta tgttggtgta
    76741 ccaatggcgg ctgcactgtc tgtgacccac ggcttcctgc caccgcatcc gggcccgact
    76801 gcgattgcca ccattttcaa tgccgatatg ggtaaaaccc tgctgtacgg tactattctg
    76861 gcaatcccga ccgtgattct cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat
    76921 aagccaatcc cggaaggtct ctacagcgcg aaaaccttca gcgaagaaga gatgccgagc
    76981 tttggcgtca gcgtctggac ctctctggtg ccggttgtac tgatggcgat gcgtgcgatt
    77041 gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg tagcggagtt cctcggtgac
    77101 ccggtaatgg caacgctgat tgccgtgctg attgcgatgt tcacctttgg tctgaaccgt
    77161 ggtcgttcaa tggatcagat taacgacacg ctggtttctt ccatcaaaat cattgcgatg
    77221 atgctgttga tcatcggtgg tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg
    77281 gacaaataca ttgcttccat gatgcacgaa accaacattt ctccgctgct gatggcctgg
    77341 tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa ccgttgcggc aatcactgcg
    77401 ggtggtatcg cggcaccgct gattgcaacg acgggtgtta gcccagagct gatggttatt
    77461 gcggtgggtt ccggtagtgt gattttctct catgtgaacg atccgggctt ctggctgttc
    77521 aaagagtact ttaacctgac tatcggcgag accatcaaat cctggtcgat gctggaaacc
    77581 attatctcgg tgtgcggtct ggtaggctgt ctgctgctga atatggtgat ttgataacac
    77641 tgactgccgg atgcatcgca tccgacatca accgcactct acttcttctt cgctgcagct
    77701 ctgcgccgtc tgtccaaatc cttcagcaac ttgttcacgc catcatcggc aaacatcgac
    77761 tcaagcgttg cggaaagctt gcgtcgccag tttttatact ggtaactggt gccaggaata
    77821 ttcaccggtt cggccatatc cagccagtct tccggctgta gtcctaacag agcactgtta
    77881 ctgtcggcaa tgtagcgctg caaaccacgg ttcagcgtcg tcgtcatcga catcaacgat
    77941 gccttatgcc cggcacgttt tggcagacaa ccatatttat gcagtgcatc cagcagccct
    78001 tgcttcgcca gttcgcgatc ctgatacaga ccgcgcagta ccacttcatc cggatacagc
    78061 cccagggttt tgcccagcgt tagatccccg ctctcccagt aaccacgcag cgttggcagg
    78121 tcatgtgtcg ccgcaaccgc catcgactgc tccggatacg ctttcggtgc acggaacgtc
    78181 ttctcgtggt cgttttcgaa atagagcact ttgtaagagt acacaccgct gctgcgcagc
    78241 ttaccgacaa tctctaccgg tacggtaccg agatcttcac caatcaccat acagcgatga
    78301 cgtttacttt cgagtgccag aatcgagagc agatcatcca ccggatagtg aacatacgcg
    78361 ccctgatctg ccgtctcgcc atacggtatc caccacaaac gcagcatcga catcacatgg
    78421 tcaattcgta atgcgccgca gttttgcata ttggcacgca acagctcgat aaacggttca
    78481 taggcacgcg cggtgatgat atgcgggtcc attggcggta atccccagtt ctgccccaac
    78541 gggccgagga tatccggcgg cgcgccaacc gatgctttca ggcaatatag ttcacggtca
    78601 caccaggttt ccgccccacc ttccgctacg ccaaccgcca gatcacgata caagccaatc
    78661 ggcatttcat agccctggct tatctcccag caggcggcaa actggctgta agccagccac
    78721 tgcaaccaga gataaaaatc gacgtcatca cgatgttctt cgcagaactg acgcacttct
    78781 ggtgaatcca cgttcttata catctctggc catgcaggcc agccccagcg catttcgtcc
    78841 tctttcactt gctgggcatg tagcgcatca aaggctgcct gccagaacag gctgtcgccc
    78901 tgctctgcaa caaactggcg aaacgcggcc atctgctcat catcacgttg cgcgaaacct
    78961 ttccacgcca ttcgtaatgc tgtcattttt agggcggtaa ccgtggagta atcgacccag
    79021 tcggcatcgc gcgcctgttg cagcgtctgt tgcgtggtcg gcaactgcca ccaggcctga
    79081 gcctcttcgc taagatggaa atcttcaacg gcgttaacgt cgatataaat cacattcagc
    79141 caacggcgag aagacgggct gtatgggctg gcgctctccg gatttgccgg atagagcgca
    79201 tgaatcgggt tcaggccaat gaacgacccg ccacgttttg ccacatccac cagcatcgct
    79261 ttgagatcgc caaaatcccc aataccccag tttttttccg atcgcagcgt ataaagctga
    79321 acgcaggcac cccacagctt ttgtttattc agcaaagcct gcggttcgta acaacgtttc
    79381 ggggcgacaa tcacccggca atgcgcgcgc tggtcgtcct gggtgagtgt tagcgtgtga
    79441 taaccttccg gcagcttcgt cggtagattg aacgctttgc cccccgttac atggcctttg
    79501 tactgcgttc cttcttcggt ggtcagctgc cagctatatt cgccgctgcc ctccaccacc
    79561 atcggcattt ttttgccgct ggtataaacc atgacattcg ggactggcgt taccgccact
    79621 ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc gtttggtttc ggcgctaatc
    79681 gactgcggtt taccgtgggc attgatgtaa ttggggctaa tccccgccgc cagcgcggca
    79741 ttatccagac gtttgctttc catcgagctt ccttagcgtt ttgcctgcca gatacgagcc
    79801 tgataatcgc gaatagagcg atccgagcta aacataccgc agcgggcggt attgaggatc
    79861 gccgcgcgag tccaggcctc ctggtcgcgg tacagcacat ccacctgctt ttgtgcctca
    79921 acataggctg cgaaatccgc catcaccaga tacggatcgc cgccctgttt gccgatactg
    79981 tgcagcatct ggtcgaaggc atgcttatcg ccgtcgctgt atttaccgct ttccagctct
    80041 ttcaatactg cgtccagcac cttatctttc ttccgccatt tcaccgggtc gtagcctttg
    80101 gccagaatag ccttcacttg ttccacggta tgaccaaaaa taaagatatt ttcttcaccg
    80161 actttctcgg cgatttcaac gttcgcacca tccagcgtac cgacagtaag cgcaccattg
    80221 agcgccagtt tcatattgcc ggtaccggaa gcttctttac ctgcagtcga aatttgttcg
    80281 gagatatccg ccgccgggat cagtttttcc gccgccgaaa cgcaataatc cggcaggaac
    80341 accaccttca acttatcgcc aaccagtgga tcgttgttga tcacgtcagc cactttgttg
    80401 atcgcaaaga taatattctt cgccaggtag tagcccggtg ccgctttcgc gccgaagagg
    80461 aagacgcgcg gtacgcgatc agcctgcagg ttttcacgaa tttctttgta caacgccaga
    80521 atatgcagca gattcaggtg ctggcgtttg tactcatgca gacgtttgat ctgaatatcg
    80581 aaaatcgcct gtggattgat ctcaataccg gtacgaactt tcacaaactc cgccagacgg
    80641 actttattcg cctgcttgat ctcgcgatat tgctgacgga atttcgcatc atcagcgaat
    80701 ttttccagat tgatcagctg atcgagatcg ttagcccact ctttttgcag tgatttatcc
    80761 aacagagccg ccagtgcagg gttgcactgt ttgatccagc gacgtggggt aataccgttg
    80821 gtgacgttat ggaatttgtt cggccatagc tggtgatatt ccgggaacag atctttcacc
    80881 accagatccg agtgcagcgc cgcaacaccg ttcaccgcga aaccgccaac cacacacagg
    80941 ttcgccatat gcacttgttt atcgtgcacc accgccagtt tggcccacac tttttcatcg
    81001 cccggccagg ttttctccac cagcgtttta aagcgagtat taatttcgtt aataatctgc
    81061 atatggcgcg gcagtaagcc tttcaccagt ttcacatccc agcgttccag cgcttctggc
    81121 atcagggtat ggttggtgta agcgaaagtt ttgctggtga tggcccaggc gtcatcccag
    81181 ctcatctggt gctcatcggt cagcacgcgc agcagttctg gaatcgcgat agttggatgg
    81241 gtatcgttca gctgaataac ttcgtaatcc gccagttcgt gcagtttacg ccccgccaga
    81301 tgatggcgac gcaaaatatc cgctaccgaa caggcacact ggaagtattg ctgcatcagg
    81361 cgcagctttt taccggcagt atggttgtcg tttggataga gaactttggt cagtttttcc
    81421 gcattgatgc cttgctgttc ggcgcgcagg aaatcaccgt cgttaaattt agttagatca
    81481 aacggatgcg catgcgtcgc ctgccacaga cgcagcggct gcgccacgcc attacgatag
    81541 ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa actccggctc ccagcgtccg
    81601 tctttcatca ctttaccgcc aatccctacc tgcacatcca gtgcttcgtt gtggcggaac
    81661 cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa cctgtttgcc atcgacaaaa
    81721 gactggcgga acaaaccata ttgatagttc agaccgtaac ccgtcgcaga ctgaccgaca
    81781 gttgccattg agtcgaggaa gcacgccgcc agacgtccca gaccaccgtt acccagcgcc
    81841 gggtcgatct cttcttccag caggtcagtc aggttgatgt cataagcctt caacgaatcc
    81901 tgtacatcct gataccagcc gagattcaac aggttgttgc ccgtcagacg accaatcaaa
    81961 aactccattg agatgtagtt aacatgtcgc tgattcgcca ccggtttggc gaatggctga
    82021 gcacgcagca tttcggccag tgcttcactc actgccagcc accactggcg aggagtcatt
    82081 tcagccgcag aatttaagcc ataacgctgc cactgacgtg aaagcgcttc ctgaaattgc
    82141 ttatcgttaa aaataggttg tgacatagga gttccacttt tcttagattt tcaacacaac
    82201 gttatcgcta gtttgccagg ctcgatgttg accttcctca tcctgcgggg gattaggcag
    82261 ggaggagttg cggggatgag caaggaaatg
//
LOCUS       DAJTRQ010000005        68808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000005 DAJTRQ010000000
VERSION     DAJTRQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(35..895)
                     /gene="rsmI"
                     /locus_tag="OO332_000419"
     CDS             complement(35..895)
                     /gene="rsmI"
                     /locus_tag="OO332_000419"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5941022.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            960..2996
                     /gene="lpoA"
                     /locus_tag="OO332_000420"
     CDS             960..2996
                     /gene="lpoA"
                     /locus_tag="OO332_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5941023.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            2954..3349
                     /locus_tag="OO332_000421"
     CDS             2954..3349
                     /locus_tag="OO332_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5941024.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3369..3959
                     /gene="diaA"
                     /locus_tag="OO332_000422"
     CDS             3369..3959
                     /gene="diaA"
                     /locus_tag="OO332_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5941025.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            3969..4544
                     /gene="yraP"
                     /locus_tag="OO332_000423"
     CDS             3969..4544
                     /gene="yraP"
                     /locus_tag="OO332_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5941026.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4658..5698)
                     /locus_tag="OO332_000424"
     CDS             complement(4658..5698)
                     /locus_tag="OO332_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5941027.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5771..6406)
                     /locus_tag="OO332_000425"
     CDS             complement(5771..6406)
                     /locus_tag="OO332_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5941028.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6534..7052
                     /gene="yhbO"
                     /locus_tag="OO332_000426"
     CDS             6534..7052
                     /gene="yhbO"
                     /locus_tag="OO332_000426"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5941029.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7032..7475)
                     /locus_tag="OO332_000427"
     CDS             complement(7032..7475)
                     /locus_tag="OO332_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5941030.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7526..7828
                     /gene="yhbQ"
                     /locus_tag="OO332_000428"
     CDS             7526..7828
                     /gene="yhbQ"
                     /locus_tag="OO332_000428"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5941031.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(7815..8318)
                     /locus_tag="OO332_000429"
     CDS             complement(7815..8318)
                     /locus_tag="OO332_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5941032.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8312..8836)
                     /locus_tag="OO332_000430"
     CDS             complement(8312..8836)
                     /locus_tag="OO332_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5941033.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9045..10040
                     /locus_tag="OO332_000431"
     CDS             9045..10040
                     /locus_tag="OO332_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5941034.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10049..10927
                     /locus_tag="OO332_000432"
     CDS             10049..10927
                     /locus_tag="OO332_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5941035.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11008..12015
                     /locus_tag="OO332_000433"
     CDS             11008..12015
                     /locus_tag="OO332_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5941036.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12133..13377)
                     /gene="mtr"
                     /locus_tag="OO332_000434"
     CDS             complement(12133..13377)
                     /gene="mtr"
                     /locus_tag="OO332_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5941037.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13531..15420)
                     /gene="deaD"
                     /locus_tag="OO332_000435"
     CDS             complement(13531..15420)
                     /gene="deaD"
                     /locus_tag="OO332_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5941038.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15413..15493)
                     /gene="yrbN"
                     /locus_tag="OO332_000436"
     CDS             complement(15413..15493)
                     /gene="yrbN"
                     /locus_tag="OO332_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5941039.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15600..16484)
                     /gene="nlpI"
                     /locus_tag="OO332_000437"
     CDS             complement(15600..16484)
                     /gene="nlpI"
                     /locus_tag="OO332_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5941040.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16593..18728)
                     /gene="pnp"
                     /locus_tag="OO332_000438"
     CDS             complement(16593..18728)
                     /gene="pnp"
                     /locus_tag="OO332_000438"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5941041.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(18975..19244)
                     /gene="rpsO"
                     /locus_tag="OO332_000439"
     CDS             complement(18975..19244)
                     /gene="rpsO"
                     /locus_tag="OO332_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5941042.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19393..20337)
                     /gene="truB"
                     /locus_tag="OO332_000440"
     CDS             complement(19393..20337)
                     /gene="truB"
                     /locus_tag="OO332_000440"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5941043.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20337..20738)
                     /gene="rbfA"
                     /locus_tag="OO332_000441"
     CDS             complement(20337..20738)
                     /gene="rbfA"
                     /locus_tag="OO332_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5941044.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(20902..23550)
                     /gene="infB"
                     /locus_tag="OO332_000442"
     CDS             complement(20902..23550)
                     /gene="infB"
                     /locus_tag="OO332_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5941045.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23575..25062)
                     /gene="nusA"
                     /locus_tag="OO332_000443"
     CDS             complement(23575..25062)
                     /gene="nusA"
                     /locus_tag="OO332_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5941046.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25090..25542)
                     /gene="rimP"
                     /locus_tag="OO332_000444"
     CDS             complement(25090..25542)
                     /gene="rimP"
                     /locus_tag="OO332_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5941047.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25749..25825)
                     /locus_tag="OO332_000445"
     tRNA            complement(25749..25825)
                     /locus_tag="OO332_000445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25789..25791),aa:Met,seq:cat)
     gene            26173..27516
                     /gene="argG"
                     /locus_tag="OO332_000446"
     CDS             26173..27516
                     /gene="argG"
                     /locus_tag="OO332_000446"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5941048.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27524..29148)
                     /locus_tag="OO332_000447"
                     /pseudo
     CDS             complement(27524..29148)
                     /locus_tag="OO332_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29607..29693)
                     /locus_tag="OO332_000448"
     tRNA            complement(29607..29693)
                     /locus_tag="OO332_000448"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29657..29659),aa:Leu,seq:gag)
     gene            complement(29708..30040)
                     /gene="secG"
                     /locus_tag="OO332_000449"
     CDS             complement(29708..30040)
                     /gene="secG"
                     /locus_tag="OO332_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5941049.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30268..31605)
                     /gene="glmM"
                     /locus_tag="OO332_000450"
     CDS             complement(30268..31605)
                     /gene="glmM"
                     /locus_tag="OO332_000450"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5941050.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31598..32446)
                     /gene="folP"
                     /locus_tag="OO332_000451"
     CDS             complement(31598..32446)
                     /gene="folP"
                     /locus_tag="OO332_000451"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5941051.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32536..34470)
                     /gene="ftsH"
                     /locus_tag="OO332_000452"
     CDS             complement(32536..34470)
                     /gene="ftsH"
                     /locus_tag="OO332_000452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5941052.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34570..35199)
                     /gene="rlmE"
                     /locus_tag="OO332_000453"
     CDS             complement(34570..35199)
                     /gene="rlmE"
                     /locus_tag="OO332_000453"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5941053.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35325..35618
                     /gene="yhbY"
                     /locus_tag="OO332_000454"
     CDS             35325..35618
                     /gene="yhbY"
                     /locus_tag="OO332_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5941054.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(35774..36250)
                     /gene="greA"
                     /locus_tag="OO332_000455"
     CDS             complement(35774..36250)
                     /gene="greA"
                     /locus_tag="OO332_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5941055.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36498..37931
                     /gene="dacB"
                     /locus_tag="OO332_000456"
     CDS             36498..37931
                     /gene="dacB"
                     /locus_tag="OO332_000456"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5941056.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38117..39289)
                     /gene="cgtA"
                     /locus_tag="OO332_000457"
     CDS             complement(38117..39289)
                     /gene="cgtA"
                     /locus_tag="OO332_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5941057.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39305..40270)
                     /locus_tag="OO332_000458"
     CDS             complement(39305..40270)
                     /locus_tag="OO332_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5941058.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40397..40654)
                     /gene="rpmA"
                     /locus_tag="OO332_000459"
     CDS             complement(40397..40654)
                     /gene="rpmA"
                     /locus_tag="OO332_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5941059.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40675..40986)
                     /gene="rplU"
                     /locus_tag="OO332_000460"
     CDS             complement(40675..40986)
                     /gene="rplU"
                     /locus_tag="OO332_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5941060.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41245..42216
                     /gene="ispB"
                     /locus_tag="OO332_000461"
     CDS             41245..42216
                     /gene="ispB"
                     /locus_tag="OO332_000461"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5941061.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42444..42722
                     /gene="sfsB"
                     /locus_tag="OO332_000462"
     CDS             42444..42722
                     /gene="sfsB"
                     /locus_tag="OO332_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5941062.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42770..44029)
                     /gene="murA"
                     /locus_tag="OO332_000463"
     CDS             complement(42770..44029)
                     /gene="murA"
                     /locus_tag="OO332_000463"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5941063.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44084..44338)
                     /gene="ibaG"
                     /locus_tag="OO332_000464"
     CDS             complement(44084..44338)
                     /gene="ibaG"
                     /locus_tag="OO332_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5941064.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44498..44791)
                     /gene="mlaB"
                     /locus_tag="OO332_000465"
     CDS             complement(44498..44791)
                     /gene="mlaB"
                     /locus_tag="OO332_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5941065.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44791..45426)
                     /gene="mlaC"
                     /locus_tag="OO332_000466"
     CDS             complement(44791..45426)
                     /gene="mlaC"
                     /locus_tag="OO332_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5941066.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45445..45996)
                     /gene="mlaD"
                     /locus_tag="OO332_000467"
     CDS             complement(45445..45996)
                     /gene="mlaD"
                     /locus_tag="OO332_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5941067.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46001..46783)
                     /gene="mlaE"
                     /locus_tag="OO332_000468"
     CDS             complement(46001..46783)
                     /gene="mlaE"
                     /locus_tag="OO332_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5941068.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46791..47600)
                     /gene="mlaF"
                     /locus_tag="OO332_000469"
     CDS             complement(46791..47600)
                     /gene="mlaF"
                     /locus_tag="OO332_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5941069.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            47810..48775
                     /locus_tag="OO332_000470"
     CDS             47810..48775
                     /locus_tag="OO332_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5941070.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48789..49775
                     /gene="kdsD"
                     /locus_tag="OO332_000471"
     CDS             48789..49775
                     /gene="kdsD"
                     /locus_tag="OO332_000471"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5941071.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49796..50362
                     /gene="kdsC"
                     /locus_tag="OO332_000472"
     CDS             49796..50362
                     /gene="kdsC"
                     /locus_tag="OO332_000472"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5941072.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50359..50934
                     /gene="lptC"
                     /locus_tag="OO332_000473"
     CDS             50359..50934
                     /gene="lptC"
                     /locus_tag="OO332_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5941073.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            50903..51460
                     /gene="lptA"
                     /locus_tag="OO332_000474"
     CDS             50903..51460
                     /gene="lptA"
                     /locus_tag="OO332_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5941074.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51467..52192
                     /gene="lptB"
                     /locus_tag="OO332_000475"
     CDS             51467..52192
                     /gene="lptB"
                     /locus_tag="OO332_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5941075.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52240..53673
                     /gene="rpoN"
                     /locus_tag="OO332_000476"
     CDS             52240..53673
                     /gene="rpoN"
                     /locus_tag="OO332_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5941076.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53696..53983
                     /gene="hpf"
                     /locus_tag="OO332_000477"
     CDS             53696..53983
                     /gene="hpf"
                     /locus_tag="OO332_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5941077.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54101..54592
                     /gene="ptsN"
                     /locus_tag="OO332_000478"
     CDS             54101..54592
                     /gene="ptsN"
                     /locus_tag="OO332_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5941078.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54638..55492
                     /gene="rapZ"
                     /locus_tag="OO332_000479"
     CDS             54638..55492
                     /gene="rapZ"
                     /locus_tag="OO332_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5941079.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55489..55761
                     /gene="npr"
                     /locus_tag="OO332_000480"
     CDS             55489..55761
                     /gene="npr"
                     /locus_tag="OO332_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5941080.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            55974..56606
                     /gene="yrbL"
                     /locus_tag="OO332_000481"
     CDS             55974..56606
                     /gene="yrbL"
                     /locus_tag="OO332_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5941081.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56603..57331)
                     /gene="mtgA"
                     /locus_tag="OO332_000482"
     CDS             complement(56603..57331)
                     /gene="mtgA"
                     /locus_tag="OO332_000482"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5941082.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57328..57981)
                     /gene="elbB"
                     /locus_tag="OO332_000483"
     CDS             complement(57328..57981)
                     /gene="elbB"
                     /locus_tag="OO332_000483"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5941083.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58211..60547)
                     /gene="arcB"
                     /locus_tag="OO332_000484"
     CDS             complement(58211..60547)
                     /gene="arcB"
                     /locus_tag="OO332_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5941084.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60643..61572)
                     /locus_tag="OO332_000485"
     CDS             complement(60643..61572)
                     /locus_tag="OO332_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5941085.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62247..66707
                     /gene="gltB"
                     /locus_tag="OO332_000486"
     CDS             62247..66707
                     /gene="gltB"
                     /locus_tag="OO332_000486"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5941086.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66720..68138
                     /gene="gltD"
                     /locus_tag="OO332_000487"
     CDS             66720..68138
                     /gene="gltD"
                     /locus_tag="OO332_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5941087.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68322..68801
                     /locus_tag="OO332_000488"
     CDS             68322..68801
                     /locus_tag="OO332_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5941088.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16807 a        18705 c        17095 g        16201 t
ORIGIN      
        1 gttgcgtttt gattaacgca acgcttcggc actgttaccc ctgctgctcc agcgcatact
       61 tatacagcgc atttttcttc acgccatgaa tttctgcggc cagcgccgcc gcttttttca
      121 gcggcagttc tgcctgtagc agcgccagcg tgcgcagggc atcggcgggt aagtcttctt
      181 cctgtgcttt atgaccttcg acaatcagca ccatttcgcc tttgcgacgg ttttcatctt
      241 cctttaccca cgccagcagc tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg
      301 tcagctcacg cgccagaacc acgtagcggg attcgcctaa taccgcaacg atatcttcca
      361 ggctatctaa cagacggtgg gtagattcat aaaaaatcag cgtgcgcggc tccgcttcaa
      421 tggcttttag cgcatcacgg cggccttttg atttggcagg taaaaagcct tcgtaacaga
      481 aacggtcaga tggtaaaccc gcggcgctta acgcagtgat agcagcacac ggtccgggta
      541 gcggcaccac acgaatcccc gcttcacggc aggtacgcac cagatggtag ccaggatcgt
      601 taattagcgg cgttccggca tcggaaacca gcgcaatgtt ttgcccctct tgcagcttcg
      661 ccagcagcgt ttcggctttt tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat
      721 taatcccaaa atgttgcagc aataaaccgg tgtgacgagt atcctcggcg gcaatcagat
      781 caacggcctg taatacctct aacgtacgct gggtgatatc cgccagattg ccgattggcg
      841 tcggtacaat gtaaagctgg ccctgagaat tatccgccga ttggtgttgt ttcattgtgt
      901 cgtccgtatt gccgatttaa tattgagcat tgcgtaaaaa aaatatcact ggatacatta
      961 tggtaccctc aacattttct cgtttgaaag ccgcgcgttg tctgcctgtt gttctggcag
     1021 ccctgatttt cgccggttgt ggcacccata ctcccgatca gtccactgct tatatgcagg
     1081 gcacggcgca ggctgattct gccttttatc ttcagcagat gcagcaaagc tctgatgata
     1141 ccaggatcaa ctggcaatta ctcgccattc gtgcactggt gaaagaaggt aaaaccgggc
     1201 aggcggttga gttgtttaac caactaccgc aagaactgaa cgattctcag cgtcgcgaga
     1261 aaacactgct ggcggcagag attaaactgg cgcagaaaga ttttgctggc gcgcaaaact
     1321 tgctggcgaa aatcacacct gccgatttag aacaaaacca gcaagcgcgt tactggcagg
     1381 caaaaatcga tgccagccag gggcgtcctt ccattgattt actgcgcgcg ttaattgctc
     1441 aggaaccgct gcttggcgcg aaagaaaaac agcagaatat tgatgccacc tggcaggcgc
     1501 tctcctccat gactcaggaa caggcgaata cgctggtgat caacgccgac gaaaatattc
     1561 tgcaaggctg gctggctctg cagcgcgtct ggtttgataa ccgtaacgat cccgacatga
     1621 tgaaagccgg gatcgccgac tggcagaaac gttatccgaa caatccgggc gcgaaaatgc
     1681 tgccaacgca gttggttaac gtaaaagcgt ttaaaccagc ctcgaccaac aaaatcgccc
     1741 tgctgttgcc gctgaatggc caggcagcgg tatttggtcg cactattcag caaggctttg
     1801 aagccgcgaa aaatatcggc actcagccag tggcggctca ggtagctgcc gcacctgccg
     1861 cagacgtagc agaacaacct cagccgcaaa ctgcggatgg cgttgccagc ccggcacaag
     1921 cctcggttag cgatctgacc ggtgatcagc ctgcggccca gccggtgcct gtaagcgccc
     1981 cggcgacaag caccgcagcg gtaagcgcac ccgcaaatcc atccgcagag ctgaaaatct
     2041 acgatacctc atcacaacca cttagccaga tcttaagcca ggttcagcag gatggcgcga
     2101 gtattgtggt tggtccgttg ctgaaaaata acgttgaaga gttgctgaag agcaacaccc
     2161 cgctgagcgt actggcactg aaccagccgg agaatatcga aaaccgcgtc aatatttgtt
     2221 acttcgcgct ttcaccggaa gacgaagcgc gcgatgcagc gcgtcatatt cgtgaccagg
     2281 gtaaacaagc gccgctggtg ctgatcccac gcagtgcatt gggcgatcgc gtagccaatg
     2341 cgtttgcgca agagtggcag aaactgggcg gcggcaccgt tctgcaacaa aaatttggtt
     2401 ccaccagcga attacgcgcg ggtgttaacg gcggttctgg tattgcttta acgggtagcc
     2461 cgattactcc cagagcgaca accgactccg gcatgacgac caacaatcca acgctgcaaa
     2521 ccacgccaac cgatgaccag ttcaccaata atggcggtcg tgtcgatgcg gtctacattg
     2581 tggcaacgcc gggtgaaatc gcttttatca aaccgatgat cgccatgcgt aacggtagcc
     2641 agagcggtgc aacgctgtac gccagttccc gcagcgcaca aggcaccgca ggcccggatt
     2701 tccgactgga gatggaaggc ttgcagtaca gcgaaatccc gatgctggca ggcggtaatc
     2761 tgccgttaat gcagcaggcg ctcagcgcgg tgaacaacga ttattcgctg gctcgcatgt
     2821 atgcgatggg cgttgatgcc tggtcgttgg caaatcattt ctcacaaatg cgccaggttc
     2881 agggttttga aatcaacggt aataccggaa gtctgacggc taacccggat tgcgtgatta
     2941 acaggaagtt atcatggcta cagtaccaac aaggtcaggt agtccccgcc agttaaccac
     3001 caaacagacc ggcgatgcgt gggaagcaca agcgcgtcgc tggctggaag gcaaaggact
     3061 gcggtttatc gccgctaacg tgaacgagcg tggcggcgag atcgatctga taatgcgtga
     3121 aggcctgacc accgtttttg tcgaggtacg ctatcgccgc tctgcgcttt atggcggcgc
     3181 ggcagccagt gtgacccgca gcaaacaaca caaattatta cagactgccc gcttgtggct
     3241 cgcgcgtcat aatgggagtt ttgatactgt ggattgccgg ttcgatgtgg tagccttcac
     3301 cgggaatgag gttgagtgga ttaaggatgc ctttaatgac cactcataat taaggtttaa
     3361 ggattagcgt gcaagaaaga attaaagctt gcttcactga aagcattcaa actcaaattg
     3421 cggcggcaga ggcgcttccg gatgccatct cccgtgcagc catgacgctg gttcagtctc
     3481 tgctcaatgg caacaaaatc ctctgttgtg gtaatggaac ttccgctgcc aatgcacagc
     3541 attttgctgc cagcatgatc aaccgtttcg aaacggagcg gcccagctta cctgccattg
     3601 cactaaatac tgataatgtt gtcttaacgg cgattgccaa cgatcgctta catgatgaag
     3661 tgtatgcaaa acaggtgcgg gcgctgggtc atgcgggaga tgtattgtta gccatttcca
     3721 cccgtggcaa cagccgcgat attgttaaag cagttgaagc cgccgttacg cgtgatatga
     3781 ccattgtggc attgaccggc tatgacggcg gcgaacttgc aggtttgtta gggccacagg
     3841 atgtggagat ccgcattcct tcgcatcgta gtgctcgcat tcaggaaatg catatgctga
     3901 cggtaaattg cctgtgcgat ctgatcgata acacgctttt ccctcaccag gatgattaag
     3961 gagaatacat gaaggcatta tcgccaatcg cagtccttat ttccgcgctg ctgttgcaag
     4021 gttgtgttgc cgctgccgta gtgggtaccg ctgctgtggg taccaaagcc gcaactgacc
     4081 cacgcagtgt cggcacccag gtggacgatg gtaccctgga agtgcgcgtg aacagcgcat
     4141 tgtcgaaaga cgaacagatt aagaaagaaa cccgtattaa tgtcacggcc tatcagggca
     4201 aagtgctgct ggttgggcag tcaccaaatg ctgaactttc ggctcgcgcc aaacagattg
     4261 ctatgggcgt agacggtgcc aacgaagtgt ataacgagat tcgtcagggc cagccgattg
     4321 gtctgggcga agcatctaac gatacgtgga tcaccaccaa agtgcgttcg cagctcttaa
     4381 ccagcgacct ggtgaaatcg tccaacgtga aagtgaccac cgaaaacggt gaagtgttcc
     4441 tgatggggct ggtaactgaa cgtgaagcga aagcggcggc agatattgcc agccgggtga
     4501 gcggcgtaaa gcgggtaacc acagcgttta cgtttattaa atagcagccc cttgtaatgc
     4561 ctgatgcgac gcttgccgcg tcttatcagg cctacaacga tgcaaaccgt aggtcggata
     4621 aggcgtttac gccgcatccg acatgttttc cctcaaatca gaacaacagc gccagcccgc
     4681 cgacaatcac accagacact gccaccatcg cccctgtcag ccataaggct ttcgccggga
     4741 acgctttgcg cagcataatc agtgacggca aactcactgc cgggagcgtc atcaacaatg
     4801 ccagcgccgg agcggttccc atacctgcca gcatcatcgt ttgtacaatc ggaatttctg
     4861 ctgccgtggg aatgacaaac aagcatcctg ctaccgccat cgccaccacc cacatcaggc
     4921 tgttatcgac agcaccatcg gcatggggga ataaccagac gcgtgcggca cccaacacca
     4981 gtactgcaag gatgtaaacc gggatcgtac tccagaaaag tgtccatagc gccctgcccc
     5041 agcggctaaa aaaaccgctc tgtgcttccg gtatgtcaat ttcgaccggt gcctgcgttt
     5101 gcggtgtttc acgcacccat ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc
     5161 cggccaccag acgaatcgcc gcaaaacccc agccgaggac aaaccccata aacaccagcg
     5221 tcgccgggtt taacaccgga ttgcccatcc agaatgccag cgcaccgccc atcgatacct
     5281 gttggcgacg cattcccgcc gcgaccggag ccgcacagca ggtacacatc atgcccggca
     5341 gcgaaaacag cgttcccggc agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca
     5401 accagtcacg cgggatcagc acctgaatca acgaaccgag gatcaccccc agcaccgccg
     5461 ctttccatac tgcgaggaaa tagatcatcg cgtaatccaa cgccgcttgc cacggattag
     5521 catccgcctg cgcaaggata gatttaccga tactgtgggt ttcggctgca gtaaacgctt
     5581 tgccgtagta aggttgccat ttcacatacc agagaccggc aatgacaacg agaaagaaaa
     5641 gcgcgggttt ccaccactga atgggcgttg ccgcctgaga tgaagactga ccagtcatag
     5701 cattccccag gaataagtta tgataattga gcgcgtgaat attacgctca ctatcaattc
     5761 ttgggaataa ttattcagct cttttgcgta attctgaaga gcttaaaatc gtcacgcctt
     5821 catgctcagg ccgcatcgcc tctgccagca tgacccgcgc cacgtctctc gcatcaatgg
     5881 atttccagtt acctggtaac aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct
     5941 gtttgctacg atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc ggccagttct
     6001 gggcaattaa tgcttcttcc atctcccctt tgacgcggtt atagaaaaac ggcgagtggg
     6061 cattggcacc catagcactg accaccagca tatgctgcgc gcccagtcgc cgcccggtta
     6121 atgcggtatc cactaccagc gtgtaatcgg catgaataaa cgcctctttg ctccccgctt
     6181 ctcgccgcgt ggtgccgaga caacaaaata caatgtcgat gggttcggtg acctgcgcca
     6241 acgcgtcact cagttgcgga tcatggggat taaacacccc gggcatatcg cccaacggac
     6301 gtcgcgtcgg cgcagcaatg gcgttaactt tcggttcatt aatcaacatc cgcagcaggt
     6361 gaccgcccac cagccccgtt gcgcctgtaa tcagtacctg actcatcttc gctcctttac
     6421 agaattgtct gccttgcgct ccccggctca tgcaccagtc ttaatagacc atgaggtaat
     6481 tatccccgat tgtggaaaat tcgcctcatc caatgcaaca acgcggagga agcatgagta
     6541 agaaaattgc cgttttaatc actgatgaat ttgaagattc agaatttact tcacccgcag
     6601 acgagttccg taaagccgga cacgaagtga ttactattga aaaacaagcg ggtaaaacgg
     6661 tgaaaggcaa aaaaggagaa gccagcgtga ccatcgataa atccatcgat gaagtgacgc
     6721 cagcggagtt tgatgccctg ttgctaccgg gtggtcattc accggattat ctgcgtggcg
     6781 acaaccgttt tgtcaccttt acccgtgatt ttgtgaatag cggcaaacct gttttcgcca
     6841 tctgtcacgg tccgcagctg ctgatcagcg ccgatgtgat tcgggggcgc aaactgaccg
     6901 cagttaaacc gatcattatt gatgttaaaa atgcgggcgt ggaattttac gatcaggaag
     6961 tcgtggttga taaagatcag ctggttacca gccggacacc ggacgatctg ccagcgttta
     7021 accgcgaggc gttacgcctg ctcggtgcct gagtcgcgta gccaaatcat ttttttacca
     7081 aagcccagcg tgttgtcggt gaatttgatt tcatcgagcc ggatttccca caccggggcc
     7141 gacagcattc tggcaaccgg aaagcgacga ttgtacgcct tgcgcgcgag gtcgctttcc
     7201 tcaccttcca gcctgcggat ctcaccttta aactgcacac cgcgaattaa cgctaccgtt
     7261 ttcggctgac cgtttaccgt tccggcaacc gcagcctgcg gcccactcat ctgcgcgtgg
     7321 cgcgtttttt cttccgtcaa aatgtagaag gcgactttct gcgcatcaaa aagataaaag
     7381 gcattagcgc accagagttc cccttcctgc tgcacacacc aggtgacaac atgttgtttt
     7441 gccagccaac ggctgatggc gatgagtgtt tccattgctg ttctctttta tactgtgggc
     7501 ctgaccttaa catgcgatgc agacgatgac cccctggttt ctttacttga tccgtaccgc
     7561 cgacaataag ctttataccg ggatcaccac ggatgtcgaa cgccgctatc agcagcacca
     7621 aagcggcaaa ggggcgaaag cactgcgcgg gaaaggagaa ctgacgctgg cgttttctgc
     7681 gccagtgggc gaccgttcgc tggcactacg ggcggaatat cgcgttaagc aactgaccaa
     7741 acggcaaaaa gagcgtctgg tagcggaagg cgcagggttt gcagaactgt tgagcagcct
     7801 gcaaaccccg gagattaaaa gcgattgaaa tgctcgtgat actcaaccag accggttacg
     7861 ccattcagcg cgtcatccgc taaacgatgt acctggaagg cgctttcagt gcccggccag
     7921 cggcaacgca gatcgtgatg cgccgccagt tcaaagccga aacgactgta cagcgccgga
     7981 tcgcccagcg tcaccactgc ggcatagccg aactcattaa gcgaatcgag tccttcatag
     8041 accagttggc gtgccagccc ctgtccacgg tatttttcat caaccgccaa cggtgccatg
     8101 ccgacccatt gcaggtcttc gccctgcaca tcaaccggac taaatgccac atagccaatg
     8161 acctgacctt cgtcatctgt cgccaccagc cccagcgtca gaaagccatc ttcacgcaga
     8221 tcgtgaacca gcttcgcttc cgcatcactt tcgaatgagc gacgcagcag ggcatcaata
     8281 cccggcgcat caatgggaat ttctactcga attagcatgg ttcacctacc gatgtctgtt
     8341 tggtttcagg cgcggttttc attcccgcct caacaaaatc cgccagttgc agcagcatca
     8401 tgcgtaaggc tttcggcatt tgctccagtt caatggcgtc catcaggttt ttcacataca
     8461 cccccagctc cgtatcgcct tcaatcacca gccgacgctg gaagaagagc gtatccggat
     8521 cttgtttacg cgccgcaatc atcagcagat cgctggcgtc ggcactaaaa ctcacatcag
     8581 cttgcgcgtt ctggctgacc accagtttgc cattcaccac cgaggtaaac cattgcaggt
     8641 caatatcaca cacatgaata cttaaccagc ggccttcaag aaactccagc tcgccatcat
     8701 ccagcgcctg acggaattgc cagcttaaga cctgctcaag aacctggcgt tttagcgcaa
     8761 atggcgtcag ttttaccggt acactcaaca gagatggccc caaatgcaca atacgggaac
     8821 gcagtttatc caacacgagt tttactccct gtttcaacaa tcatcctatt ttgccatatc
     8881 agaaaaataa catagcggta taaatcaaca attccatatg aaattgctgc taccaccaat
     8941 acaactttaa ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa atcccagttc
     9001 gttaacattt ttgcgttttg atagcgcaac cttcaggaaa aattatggag ctgctctgcc
     9061 ctgccggaaa tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata
     9121 tcgggctaaa agatgatacc aatgcccgtc acttcgccgg ccttaacttt accgagaaaa
     9181 aattgcagga agcggtgagt tttgtccatc aacatcgccg caaacttcac atctcgatta
     9241 acacttttgc gcatccggac ggttacgccc gttggcagcg tgccgtggat atggcggcgc
     9301 agctgggtgc cgacgcgctg atcctcgccg acctcgccat gctggagtac gccgccgagc
     9361 gttacccgca tattgaacgt catgtgtcag tgcaggcttc ggcgaccaat gaagaggcga
     9421 ttaactttta tcatcgccat tttgacgttg cccgcgtggt gctgccgcgc gtgttgtcga
     9481 ttcatcaggt gaaacagctg gcacgggtta ctcctgtccc gctggaagtg tttgctttcg
     9541 gcagcctgtg cattatgtcg gaaggccgtt gctatctgtc gtcgtatctg acgggtgagt
     9601 cgcccaacac tgtgggtgcg tgttctccgg cccgtttcgt gcgctggcaa caaacgccgc
     9661 aggggctgga atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg
     9721 caggttatcc gacgctgtgt aaagggcgtt atctggtgga cggcgagcgc tatcacgcgc
     9781 tggaagaacc aaccagtctc aataccctgg aactgctgcc ggagttaatg gctgcgaata
     9841 ttgcttcggt gaaaattgaa ggccgccagc gtagcccggc gtatgtcagc caggtggcga
     9901 aagtctggcg tcaggctatc gaccgttgta aggccgatcc gcaaaacttc gtaccgcaaa
     9961 gcgcgtggat ggagacgctc gggtcgatgt ccgaaggcac gcaaaccacc cttggcgcgt
    10021 atcaccgtaa atggcagtga gaagagcaat gaaatattcc ttagggccag tgctgtggta
    10081 ctggccaaaa gagacgctgg aagattttta tcagcaagcc gccgccagca gcgccgacgt
    10141 gatttatctt ggtgaagcgg tatgcagcaa gcgccgggca accaaagttg gcgactgtct
    10201 ggagatggca aaatcgctcg ccggaagtgg caagcagatt gtgctctcca cgctggcgct
    10261 ggtgcaggca tcctctgaac tgggcgaact gaaacgctat gttgagaacg gtgagtttct
    10321 gattgaagcc agcgatctcg gcgtggtgaa tatgtgcgct gaacgcaaac tgccgttcgt
    10381 cgccgggcac gcgctgaact gctacaacgc ggtgacgctg aaaatattgc tcaaacaggg
    10441 catgatgcgc tggtgtatgc cggtggagct ttcccgcgac tggctggtga atctgcttaa
    10501 tcagtgcgat gagctgggca ttcgcaacca gtttgaagtg gaagtcctga gctacggtca
    10561 tctaccgctg gcctactccg cccgctgctt taccgcgcgt tcggaagacc gcccgaaaga
    10621 tgagtgtgaa acctgctgca ttaagtatcc gaacgggcgc aacgtgctgt cgcaggaaaa
    10681 ccaacaagtg tttgtactca atggcattca gaccatgagc ggctacgttt acaacctcgg
    10741 taacgagctg gcatccatgc agggattggt tgatgtagtc cgcctgtcac cgcagggcac
    10801 tgacactttc gcgatgctcg acgccttccg cgctaatgaa aatggcgctg tgccactgcc
    10861 gttgacggca aacagcgact gtaacggcta ctggcggcgg ctggcaggac tggagctgca
    10921 agcctaagta aatagctcac tttgttaaca actttaacta ttctttaatg cagtattaaa
    10981 gattaatcgg taacaaagtg agctgttatg actgataaaa ccattgcgtt ttcgctactc
    11041 gatctggccc ccatacccga aggctcttca gcgcgggaag cgttctccca ctctctcgat
    11101 ctcgcccgtc tggctgaaaa gcgcggctat catcgctact ggctggcaga acaccacaat
    11161 atgaccggca ttgccagtgc tgcaacatcg gtattgattg gctatctggc ggcgaatacc
    11221 accacgctgc atctggggtc tggcggcgtg atgttgccta accactcacc gttggtcatt
    11281 gccgaacagt tcggcacact caatacgctc tatccggggc gaatcgattt ggggctgggt
    11341 cgtgctccgg gtagtgacca acggacaatg atggcgctac gtcgtcatat gagcggcgat
    11401 attgataatt tcccccgcga tgtcgcggag ctggtggact ggtttgacgc ccgcgatccc
    11461 aatccgcatg tgcgcccggt accaggctat ggcgagaaaa tccccgtgtg gttgttaggc
    11521 tccagccttt acagtgcaca actggcggcg cagcttggtc tgccatttgc gtttgcctca
    11581 cacttcgcgc cggatatgtt gttccaggcg ctgcatcttt atcgcagtaa cttcaaaccg
    11641 tcggctcggc tggaaaaacc atacgcgatg gtgtgcatca atattatcgc cgccgacagc
    11701 aaccgcgacg ctgaatttct gtttacctca atgcagcaag cctttgtgaa gctgcgccgt
    11761 ggcgaaaccg ggcaactgcc gccgccgatt caaaatatgg atcagttctg gtcaccgtct
    11821 gagcagtatg acgtgcagca ggcgctgagt atgtcgttgg taggcgataa agcgaaagtg
    11881 cgtcatggtt tgcagtcgat cctgcgcgaa accgacgctg atgagattat ggttaacggg
    11941 cagattttcg accaccaggc gcgcctgcat tcgtttgagc tggcgatgga tgttaaggaa
    12001 gagttgttgg gatagtgtgt cttaacgcgg gaagccttat ccgagctggc aacgctgtcc
    12061 tacgtaggcc tgataagcga agcgcatcag gcattgtgta ggcagcagaa atgtcggata
    12121 aggcaccgct gattactgat acaccggcag taaattaaag ctcgataaaa tatgcaccag
    12181 tgcgttgccg acgccaaaca ccagaatcag cgcaatcatc ggcttgccac cccagacgcg
    12241 gaatttcggg ctgccaaagc gtttacgcga tgcgcgggcc agcagtgccg gaacaattgc
    12301 cgcccagatg gtagccgcta aaccagcata accaatggca tacaggaatc cgttcgggaa
    12361 cagcagacca cccacaacag gcggggcaaa ggtcagcaat gccgttttca agcggcccac
    12421 agccgagtcg tcgaaaccaa acagatctgc cagatagtca aacaaaccca gcgttacgcc
    12481 aaggaacgaa ctcgctaccg caaagtttga gaacacgacc agcagaaggt ccaggctacg
    12541 gctgttcagc acgccgctta atgcttgtac cagcacatca atattaccgc ccttctcggc
    12601 gatgccgata aactccggac gagggatgtt gcccattgtc gccagcaacc agatggtata
    12661 cagcgccagt gccatcagcg taccgtacac caggcatttc acgatggttt tcggatcttt
    12721 gccgtaatac ttcatcaggc ttggcacgtt accgtgataa ccaaacgacg ccaagcagaa
    12781 cggcagggtc atcagcaaat acggcgcata agatgcattg ctttcggcaa cgttgaacaa
    12841 tgtcgcaggc tgcacatgcc ccagcaggct accgaaggtg aggaagaagg taatgacttt
    12901 cgcccccagc acaatcgccg tcatgcggct gacggcttta gtactcaacc acaccacaaa
    12961 cgctaccagc agtgcaaaac caaaacccgc cgcccgtgcc gggacgttta gcgacatctc
    13021 tgcgaaggtg tgatgcagaa tcgaaccact ggcagaaata taggcatagg tcaggatata
    13081 gagcacaaag gcaatggaaa tgccgttgac cacgttccag cctttgccca gcaaatcttt
    13141 ggtgatggtg tcaaaactcg aaccgattcg gtaattcagg ttagcttcca gaatcatcaa
    13201 gccggaatgc agcatacaga accaggtaaa gatcagcgct gccattgacc agaaaaacca
    13261 cgccccggac atgaccactg gcagagaaaa catccctgcg ccaataatgg tgccgccgat
    13321 aatcaccacg ccgccaagca gcgacggtga cgtttgggtg gtggttagtg ttgccatgag
    13381 ggcttctctc cagtgaaaaa tagtgcgact gcgttgttat gcattgcact gtaccagtac
    13441 atgagtacaa aagacagaaa aaaagccccg atggttaaaa tcggggctgt atatattatt
    13501 ttacagattg tgttcgctgt tcagcgatga ttacgcatca ccaccgaaac gacgacgacc
    13561 ggtagagtca tcacgacgcg gtgcgcggcc ttcacgacgt tcgccgctaa aacgacgacc
    13621 atcaccacga ccaccttcac ggcgttcacc gctgaagtta cgaccgcctt cacgacgctc
    13681 gccaccgaaa ccacgaccac cgccacgaag ctcaccgcca gtatgcggct gtgcatcgcc
    13741 cagtaactgc atgttcatcg gcttgttgag aatgcgagtg cgcgtaaagt gttgcagcac
    13801 ttcacccggc atacctttcg gcagttcgat ggtggagtga gaagcaaaca gcttgatgtt
    13861 accgatgtaa cggctgctga tgtcgccttc gttagcaatc gcgccaacga tatgacgaac
    13921 ttcaacacca tcatcgcggc ccacttcaat gcggtacagc tgcatatcgc caacatcacg
    13981 acgttcacga cgcggacgat cttcacggtc gccacgcggg ccacggtcgt tacgatcgcg
    14041 cggaccacgg tcatcacggt cacggaattc acgtttcgga cgcatcggcg catctggcgg
    14101 aacaatcaga gtacgttcac cctgtgccat tttcagcagt gccgcagcca gagtttcgag
    14161 atccagctct tcaccttctg cagtcggctg aattttgctc agcagtgcgc ggtattgatc
    14221 cagatcgctg ctttccagct gctgctgtac tttagcggcg aatttttcca gacggcgttt
    14281 gcccagcagt tctgcgttcg gcagttctac ttccggaata gtcagcttca tagtacgttc
    14341 aatgttgcgc agcagacgac gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg
    14401 acccgcacga ccggtacgac cgatacggtg aacgtaagac tcagaatcca tcgggatatc
    14461 gtagttaacc accaggctga tacgctcaac gtccaggcca cgggccgcaa cgtcggtcgc
    14521 aatcaggatg tccagacgac cgtctttaag gcgttccagt gtctgctcac gcagcgcctg
    14581 gttcatgtca ccgttcagcg cggcgctatt gtagccgtta cgctcaagag cttcagccac
    14641 ttccagagtc gcgtttttgg tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc
    14701 caggaaacgt accagtgctt cgtttttacg cataccccac acagtccagt agctctggct
    14761 gatgtcagga cgggtagtca cgctggactg aatacgcact tcctgcggct ctttcataaa
    14821 gcggcgggta atgcgacgaa tcgcttccgg catggttgca gagaacagag cggtctgatg
    14881 accttccggg atctgcgcca taatggtttc aacgtcttcg atgaagccca tgcgcagcat
    14941 ttcgtcagct tcatccagaa ccagaccgct cagtttagag aggtccagag tgccacgttt
    15001 caggtggtcc agcagacggc ccggagtacc gacaacgatc tgcggcccct gacgcagggc
    15061 gcgtaattgc acgtcataac gctggccgcc gtacagggca accacattta cgccgcgcat
    15121 gtgtttagag aaatccgtca ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc
    15181 cagcaccaga atctgtggtg ctttcagctc aggatcaaga ttctgcagca gaggtaaaga
    15241 gaatgctgca gtttttccgc tccccgtctg ggccataccc agaacgtcgc ggccattcag
    15301 cagatgtgga atacactctg cctgaattgg agatggtttt tcgtaaccca gatcgttaag
    15361 ggcttcaagg ataggagcct tcaggcccag atctgcaaaa gtggtttcga attcagccat
    15421 gtagtacgtg tgcctcaaaa ttaatggcgg ccagtctaca taactcatca tgaaattgat
    15481 cagcaatttt cattgaaaag tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac
    15541 gccctcaccc gttaaggtga tggcaatcaa aaaagattac gggctgatgt gtacgtcagc
    15601 tattgctggt ccgattctgc caggtcatct tggtcctggc ccaggagcga taattccaac
    15661 aatgcgtatc ggtgctcaac aaagttatga acgttgttgg caaccgccag tttgaacagt
    15721 gccgtggcgc tgtccaaatc ccccagactt aggtagtact tacctaaata gaagttggtt
    15781 tcactgagat gctcagcgag cgaggtgtta tcggttgcgt ccgccttgag cctttccatt
    15841 aacgtgtgtt cgctaatgtt gcccaggtag aactcgacaa tgttccatcc ccactgttcc
    15901 ttatccgatt tttcgaagtg ctgtttcaac acttctttag cctgcttctc atcgagcttc
    15961 tgctcggcga gataaagcca cagactacgg aaaggatcat tgggatcgtc ttgataaaac
    16021 gccagcagat catcttgcgc taacttgtca cgaccgccgt aatataatgc aatcccgcga
    16081 ttcaagtgcg cgtagttgta agttggatca agctcaagta cagaatcaaa cgcttcatag
    16141 gcagcatcaa aattgcctgc ctgcgttaaa tatatgccta agtaattgaa tacttcaggc
    16201 atatccggtc ggattgccag cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg
    16261 agactatcat acaacactcc gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa
    16321 gcccgactgg caaggatttg ttccatacgt gccagaatca cttcctgctg taaagtcggt
    16381 tgcaatggta ccgcgaggac ttcactttta cgccaggaag tattactgca tcctgcaagc
    16441 gtaagtgctg tcgcaacgaa acaccagcgc aaaaaaggct tcatttccca ctcccgaaga
    16501 ccacggttga atgaacgtcc tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag
    16561 ccccccgccg cagcggaggg caaatggcaa ccttactcgc cctgttcagc agccggagct
    16621 tccggtgctg cagcaggttg agactgctca gtcgcttctt taatgctcag acggatacgg
    16681 ccctggcgat caacttccag aactttcacc ggtacttcct gacccatctg caggtaatcg
    16741 gtcactttct caacgcgttt gtcagcgatt tgagagatgt gtaccagacc ttctttaccg
    16801 ccgccgatgg caacaaatgc gccaaagtca acgatacggg tcactttacc agtgtagacg
    16861 cggcccactt cgatttctgc agtgatctct tcgatacgac gaatagcatg tttcgctttc
    16921 tcgccgtcgg tcgctgcaat cttcacagta ccgtcatctt cgatttcgat ggtggtgcca
    16981 gtttcttcgg tcagggcacg gataacagag ccgcctttac cgataacgtc tttgatcttg
    17041 tccgggttga tcttgatggt atggatacgc ggtgcgaact cagagatatc gccacgcggc
    17101 gcgttgatcg cctgttccat tacgcccagg atatgcagac gcgcaccttt agcctggttc
    17161 agcgcaacct gcatgatctc tttggtgata ccttcaattt tgatatccat ctgcaatgca
    17221 gagataccct cgcgggaacc cgcaactttg aagtccatat cgcccaggtg atcttcgtcg
    17281 cccaaaatgt cagacagtac aacgtagttg tcgccttctt tcaccagacc cattgcgata
    17341 cccgcaacgg cagctttgat cggcacacct gcgtccatca gcgccagaga cgcgccgcac
    17401 acggaagcca tagaagagga accgttggat tcagtgattt cagacacaac acgtacggtg
    17461 tacgggaatt tgtccatatc cggcatgact gccagcacgc cgcgcttcgc cagacgaccg
    17521 tgaccaattt cacgacgctt cggagaaccg accatgccgg tttcgcctac ggagtacgga
    17581 gggaagttgt agtggaacag gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc
    17641 tgcgcgtcac gagcagtacc cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg
    17701 aacagcgcag aaccgtgagt acgcggcagc acgccagtac gcacatccag accacggatc
    17761 atatcttttt cacgaccgtc gatacgcggt tcgcctgcca gtacgcggct acgaacaacg
    17821 tttttctcga tcgcgtgcag aatttcaccc agttcgtttt cgtccagggt ttcgtcttca
    17881 gcaagcagcg tcgcgatggt ttcagatttg atgacatcaa cctgcgcata acgctcttgt
    17941 ttgtcggtga tgcggtaagc atcgctcagg cgagcttcag ccagtgcagc aacgcgcgcg
    18001 ttcagcgctt cgtttaccgg ctccggctgc cagtcccaac gtggtttacc ggcttctttc
    18061 accagttcat tgatgttctg aataacaacc tgctgttgtt catgaccgaa cactactgcg
    18121 cccagcatct ggtcttcgct cagcagttca gcttcagatt caaccatcag tacagcggct
    18181 tcagtaccgg caacaaccag atccagtttg ctctctttca actcgtcctg agtcgggttc
    18241 agtacgtact ggtcattgat gtaacctacg cgagcagcac caatcggacc attgaacgga
    18301 ataccagaca gagacagtgc tgcggaagca ccaatcatcg cgacgatatc cgggttaact
    18361 tgcgggttaa cagaaaccac ggtggcgata acctgaactt cgttgacgaa gccttccggg
    18421 aacagcgggc gaatcgggcg gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttggg
    18481 cggccttcac gacggaagaa gctacccggg atacgaccag cagcgtaggt acgctcctga
    18541 tagttaacgg tcagtgggaa gaagtcctga cctggtttgg cttttttctg gccaacaacg
    18601 gtaacaaata ccgcggtgtc atccatgcta accataacag cggcagtagc ctgacgagcc
    18661 atcatgccgg tttccagagt cacggtgtgt tggccgtact ggaatttacg aacgatcgga
    18721 ttaagcaatg taatatcctt tctctttctt agacagtacc ttacggcact ggtgttaata
    18781 cccgatcttc tgcgcatcct cgcgactaat gacaacccta acccagctct atgtgggtaa
    18841 agcctctcat tagccgcgcg aacctctgca acggaagatc attcatagca acaatacatt
    18901 agtttccagt gaattgctgc cgtcagcttg aaaaaagggg ccactcaggc ccctttttct
    18961 gaaactcgca agaattagcg acgcagaccc aggcgctcga tgagctgggt gtaacgtgct
    19021 acgtctttac gtttcaggta gtcgagcagt ttacgacgct gagaaaccat gcgcagcaga
    19081 ccacgacggc tgtggtgatc ttttttgtgc tctgcaaagt ggccctgcag gtggttgatc
    19141 tgtgcagtca gcagtgctac ctgaacttca gtagaaccgg tgtcgtttgc gtcacgacca
    19201 aactcagaaa cgattttagc tgttgcttca gtacttagag acattttaaa actccaaagt
    19261 atatagaatg aaaggacgcc gatctctaat tcagcgatcc cagtgtacgt tacgcaaagt
    19321 gttaaacaat ttacgcgacg ttaagcggca gtattctact cgtagcgacc tgttatcgca
    19381 agacggttaa cattacgccg ggtattcaac caccaggcga cgaggcgcaa cgcggccttc
    19441 atcgtcaatt tcacccatac cgataaattt gccgttctca ccttctgtaa cgcgaaccag
    19501 tccttccagc ggcgcaccgg atgtacgaac tgggttacca tttttgaagt aaacagaaga
    19561 cgttaacgga agattcacca ccgggtagtc cgaagctgga ctgtccattg gcatcagtaa
    19621 tggatcaagt aactccgcag ccggaatatc ctgctgttcg gcttgttcga ccagttcacg
    19681 cagatgttcc agggtcacaa tccgttcaac cggatattta cttaccgcca gacgacgcag
    19741 gtaaataaca tgcgcgccac aaccgagttt ttcacccagg tcatcaatga tggtgcggat
    19801 ataagtgcct tttgagcagt gaatttccag ctccagctca ttgccttcgt ggcgaataaa
    19861 cagcaattca taaacggtaa tcgggcgcgc ttcacgcggt acttcaatgc cctgacgcgc
    19921 atattcgtac agttttttac cctgatattt cagtgctgaa tacatcgaag ggatctgttc
    19981 gatatcgcca cggaaagtat ccagcgccgc cgccagttgc tctgcgctaa aggttaccgg
    20041 acgttcttca acgatttgtc cgtcggcatc agaagtatcg gtacgctgtc caagacgcgc
    20101 aatgacccga tagcgtttgt cggagtccag cagatactgg gaaaacttcg tcgcttcccc
    20161 gaggcaaatt ggcaacatgc cagttgccag cgggtccagc gcaccggtat gcccggcacg
    20221 gttggcgtta taaatccgtt tcactttttg cagcgcatca ttgctggaca taccctgcgg
    20281 tttatccagc aacaaaacgc cgttaatgtc gcgaccgcga cgacgaggac gactcattag
    20341 tcctccttgc tgtcgtccgg gttaacacga cgttcttcgt catgtttgac cacgctggtc
    20401 accaggtttg acatgcgcat cccttcaacc agagagttgt cgtagaagaa ggtcagttcc
    20461 ggcacgatac gcaggcgcat cgctttcccc agcaggctgc ggatgaaacc agaagcttct
    20521 tgcaacgctt tgatgcccgc tttaaccgcg tcttcatctt tgtcgttgag gaacgtcaca
    20581 tatactttgg catacgccag gtcgcgagac atttcgacac cggaaacggt ggtcatcatg
    20641 cccaggcgag gatctttaat ttcacgctgc aggatgagag cgatctcttt ttgcatttcc
    20701 tgcgctacgc gctgcgggcg accaaattct ttcgccataa taaattctcc tgacaaaaaa
    20761 ggggctgtta gccccttttt aaaattaatt tcaggtggaa gggctgttca cgttggcctg
    20821 ataagacgcg ccagcgtcac atcaggcaat ccatgccgga tgcggcgtaa acgccttatc
    20881 ccgcatggaa ccctaaaaac cttaagcgat ggtacgttgg atctcgatga tttcgaatac
    20941 ttcgatcaca tcgccagtgc ggacgtcgtt gtagttctta acgccgatac cacattccat
    21001 accgttacgg acttcgttaa cgtcatcttt gaagcggcgc agggactcca gctcgccttc
    21061 gtagataacc acgttgtcac gcagaacgcg gatcgggttg tgacgtttaa ccacaccttc
    21121 ggtaaccata cagcctgcga tggcaccaaa tttcggcgat ttgaacacgt cacgaacttc
    21181 cgccagaccg ataatctgct gtttcagttc cggagacagc ataccgctca tcgccgcttt
    21241 cacttcgtca atcaggttat agatgacgga gtagtaacgc agatccaggc tttccgcttc
    21301 aatcacttta cgtgcagagg catcagcacg tacgttgaag ccaaccagga tggcgttgga
    21361 cgccgcagcc agggtggcgt cggtttcggt gataccacct acgccagaac cgatgatctt
    21421 cactttaact tcgtcagtag acagtttcag caaggagtcg gagatcgctt ccacagaacc
    21481 ctgtacgtct gccttcagga cgatgttcac ttcgtgaact tcgccttcgg tcatgttggc
    21541 gaacatgttc tcgagtttag atttctgctg acgcgccagt ttaacttcgc ggaatttacc
    21601 ctgacgatac agtgcaactt cacgcgcttt cttctcgtca cgtacaacgg taacttcatc
    21661 acccgcagcc ggtacgccgg acaggccgag gatttccacc ggaatggacg gacccgcttc
    21721 cagcacttcc tgacccagtt cgttacgcat cgcacgaaca cgaccgtatt cgaagccaca
    21781 cagaacgata tcgcccttgt gcagagtacc ttcacgtacc agaacggtag caaccggacc
    21841 acgaccttta tcgaggaagg attcgataac cgcaccgctc gccatacctt tacgtaccgc
    21901 tttcagctcc agaacttccg cctgcagcag gatagcgtcc agcagttcat cgataccagt
    21961 acccgctttc gcagacacat gtacgaactg gctttcaccg ccccactctt ccggcaggat
    22021 gccgtactgg gagagttcgt tcttaacgcg atccggatcg gcttctggtt tatcgatctt
    22081 gttcactgca acgaccaccg gaacctgcgc cgctttcgcg tgctggattg cttcgatcgt
    22141 ctgcggcatt acgccgtcgt cggcagcaac aaccaggact acgatgtccg ttgcctgcgc
    22201 accacgagca cgcattgaag taaacgcggc gtgccccggg gtgtccagga aggtgatcat
    22261 gccgttttca gtttcaacgt ggtatgcacc aatgtgctga gtaatgccgc ccgcttcgcc
    22321 agaggccact ttcgttgaac gaatgtagtc cagcagagag gttttaccgt ggtcaacgtg
    22381 acccatgatg gtcacaaccg gcgcgcgcgg ttcagccgca gcacccgtgt cacggtcgct
    22441 cattaccgcc tcttccagct cgttttcacg acgcaggata actttatggc ccatctcttc
    22501 agcaaccagc tgtgcggttt cctgatcgat aacctggttg atggttgcca ttgcgcccag
    22561 tttcatcatc gctttgatga cctgagagcc tttaaccgcc atcttgttcg ccagttcgcc
    22621 aacggtgata gtttcgccga tcacaacgtc acggttaacg gcctgagcag gcttctggaa
    22681 gccttgctgc agcgaagaac ctttacgttt tccgccttta ccgccacgta ctgctgcgcg
    22741 tgcttcttca cgatcagctt ttgattcagc gtgtttgttg cctttcttcg gacgcgctgc
    22801 tttcgcgtta cgaccacggc cacggccgcc ttcgacttca cgatcgcttt cgtcttctgc
    22861 ctggcgagca tgttgagaag tagtgacgtg ataatcgctg gaatcttcag tcggttccgc
    22921 gttatcagtc catttgtttt cttccgccat acgacgtgct tcttcagcaa cgcgacgtgc
    22981 ttcttcttcg agtttacgac gcgcttcttc ttcagcttta cgcttgagct ctgcagcttc
    23041 ctgctcacgg cgggcttttt cagcctgggc gtttttagtc atatcgtctt gttgattgct
    23101 cactttgtct ttttccgcag cttcacgttt cgcttgttca gcagcttcac gcttagcttg
    23161 ttctgcggcc tcacgttcag ctttttgttg cgcctcgcgt ttagccgatt cttctgcctc
    23221 acgacgggct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    23281 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    23341 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    23401 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    23461 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    23521 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    23581 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc
    23641 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    23701 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    23761 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    23821 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    23881 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga aaccttcttc
    23941 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    24001 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatta cgttgagttc
    24061 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    24121 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    24181 agcaacgtct gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag
    24241 gacgatatcg atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg
    24301 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    24361 cgcacgagaa cccgggtcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    24421 cggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    24481 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    24541 gcggaagttt tcacgcggca gcatatcttc gcgcaggatc acggcttcag cattgttgcc
    24601 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    24661 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    24721 gataacctgt tttgccgtct gggtggtgat acggtcaaag gtaacagact caatctgatc
    24781 ttcaacgtaa tcgcccaagt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    24841 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    24901 accgcttttg cgatcgatct gtacacggac gtcgatctct tgctcatatt ttttctttgt
    24961 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    25021 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    25081 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    25141 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    25201 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    25261 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    25321 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    25381 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    25441 ggatgtgcga ccgcgaataa attcgatgcc taccagttca aaacccaggg cctcaactgg
    25501 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    25561 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    25621 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    25681 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    25741 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    25801 agctaccaga ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    25861 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    25921 atagtgatcc atgccacatt ttgtcaacgt ttattgctaa tcacgtgaat gaatatccag
    25981 ttcactttca tttgttgaat acttttgcct tcccctgctc tcccttaagc gcattatttt
    26041 acaaaaaaca cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt
    26101 tattttgcat aaaaattcag tgagagcgga aatccaggct catcatcagt taattaagca
    26161 gggtgttatt ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc
    26221 gctttttccg gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagcg
    26281 gttccttatg catatactgc aaacctaggc cagccagacg aagaggatta tgatgcgatc
    26341 cctcgtcgtg ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa
    26401 ctggtggccg aaggtattgc cgctattcag tgtggcgcat ttcataacac caccggcggc
    26461 ctgacctatt tcaacacgac gccgctgggc cgcgccgtga ctggtaccat gctggttgct
    26521 gcgatgaaag aagatggcgt gaatatctgg ggtgacggta gcacctataa aggaaacgat
    26581 atcgaacgtt tctatcgtta tggtctgctg accaatgctg aactgcagat ttacaaaccg
    26641 tggcttgata ctgactttat tgatgaactg ggcggccgtc atgagatgtc tgaatttatg
    26701 attgcctgcg gtttcgacta caaaatgtct gtcgaaaaag cctactccac agactccaac
    26761 atgcttggtg caacgcatga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc
    26821 gtcaacccga ttatgggcgt gaaattctgg gatgagagcg tgaagatccc ggcagaagaa
    26881 gtcacagtac gctttgagca aggtcatccg gttgcgctga acggtaaaac ctttagcgac
    26941 gacgtagaaa tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc
    27001 gaccagattg agaaccgtat catcgaagcg aaaagccgtg gtatttacga agctccgggg
    27061 atggcactgc tgcacattgc gtatgaacgt ctgttgaccg gtattcacaa cgaagacacc
    27121 attgagcagt atcacgcgca tggtcgtcag ttgggccgtc tgctgtacca ggggcgttgg
    27181 tttgactccc aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc
    27241 actggtgaag ttaccctgga gctgcgccgt gggaacgatt attcaatcct gaataccgtc
    27301 tcagagaacc tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc
    27361 tcgccagatg atcgtattgg tcaattgacc atgcgtaatc tggatatcac tgatacccga
    27421 gagaaacttt ttggttatgc caaaactggc ctgctttcct cctctgccac ttcaggcctg
    27481 ccgcaggtgg agaatatgga aaacaaaggc cagtaattcg acatcaaccc tgcccttcag
    27541 gggtggggtt accttgctta tcctctgtaa actcctttct gagcatcaca taatcgaaaa
    27601 catcatggtt tgcatccaca acccgtgagt ctcctttata gtgtgcaatc acttcctcaa
    27661 gcgtttgcgg ctgttccggc tttgttaccc ccatccacgc attaataagg taattattgt
    27721 aagctgtcga aaagatattg ttttccgttg tgcgatccac gccatcccca agaacggggc
    27781 tataacagat aaacatcggg acatgatatg cctgctggct agcctccctg cctccatgaa
    27841 aatagacgtt cttcttcgta gggtcacgtt ccaggccgtg gtcggcaaaa tacatgaccg
    27901 aggcgcggcg atcttttaat aattcaaaaa actgacctag caaactatct gtgtaatgaa
    27961 tggagttgtc atagcaggca tcctgatcgt cctgcggttg aaacacgcgc tggattgcgg
    28021 ataggcgcta caagccggtt catggcttcc atttaaatga agaacaatca gtttcttttg
    28081 ctgcgtattt tgctgtaatg cttgcgataa atgcggcaac aacaattcat caaatcctct
    28141 gacataaact gtttccatgg cgcgcatggc gatactggta actgctgtac cattctgccg
    28201 aaaagcggat tgcgagctta gccagaaagt ctgaaatcct gcttgattag ccatattaat
    28261 aatgttgtcg gggtaattat gaatgtcatg actcaaaaca gaatcagcag ttaaagaaag
    28321 gggaaccgac agcgcagtgt aaggtgcgcc gcttattgct tgattaaaca gtttgatctg
    28381 ttttctttgt gcttcaactt gcggtgtcgt agagcgtgta tatccataca aagacatatt
    28441 gtcgacacgt acagactccc ccacaatcaa cacgtaggta tcaatacttg tatccctgac
    28501 tgataattga aaatacggca ccgtgtttgc aattgagagt aatctttgat gctctttcgc
    28561 tgctaaagca aaatagttga gattgaaaaa cggcgtatag gtagcaaatc acgacgctag
    28621 tatatatgga ctgaacgctt ttttattttt tgcatcttta taagcaaatt gacaagcgga
    28681 aaataaactg cccgaaatga caatcagcaa taatattcct gtcacttttt ttgtcggcaa
    28741 ggaaacatca tattttatta ttactgccaa aaaaaaaggg ataaaaaagc aaaagcacat
    28801 agataaggga tatacatccc cagcattttc acaacttcat ccggatcact ctgcagcaca
    28861 ctaatcgcaa atccatcatt gaaagttgta ccaaaggtac accatgaata gaggctaata
    28921 ctcatgtctg ccgcagtcag taacacaaat ggtgcggcaa caagcaggcg aagcggaagg
    28981 gatgatatac gtttaacaac caggatggtc aggacgaaga acagcaatcg ctgaaccata
    29041 tgcgctccgg aagaggcgac taagttcgta ataccaaaaa caataacaca aagatacaac
    29101 aaccaatgag atttaaaatc ttctagcaaa tttgttgaat actgtcatcc agaaatccaa
    29161 gttcacaacc aatataaaca attatcaaca atgttcaatt ttatatcaga catgtttttc
    29221 aactctgaac catatcagac cgaaacactg ttagtttttt ctttttaaat gatgcgacca
    29281 ttgcgttctc agtaattaaa taacattatc aatgcgtctc taaacattac gcagaacgta
    29341 gccatcagta gttatactga caggagcagg atggtttatc gagaagtaaa catatccgta
    29401 ttcgtaccgg aactggagtt gacacaataa agtgccaatt atgtcagcag tagggaaaag
    29461 tttataacta aggcgtaccg gcaccatcgt ttcaaggtac cagctacgag taaagcaact
    29521 ggacgagata cagatacctg acaaccattc atcagaccag gaccaaaacg aaaagagacg
    29581 cttttcagcg tctcttttct ggaatttggt accgaggacg ggacttgaac ccgtaagccc
    29641 tattgggcac taccacctca aggtagcgtg tctaccaatt ccaccacctc ggcacggata
    29701 ctacttttta gttcgggata tcgctggtcg gcttagccgg agcagctggc tgagtttgtt
    29761 cggttttcgc cggtgcactc agattttccc attcgctacc tctattggtt ttgttgctat
    29821 tgatgttacc cagcaccaga ctgatgatga agaataacgt tgccagcagc gccgtcatgc
    29881 gggtcatgaa gttaccagaa ccacttgaac caaacagcgt agcggaagcg cctgctccga
    29941 aggaggctcc catatcagcg cctttacctt gctgcagcat gatcagacca acaaggccaa
    30001 ttgccacaat aaggaaaact actaaaagag cttcgtacat aatcaacctg ttccttgcgg
    30061 agttgccgcg taccaatgct tcaaccaata aagcgggagt ttttaatctt tcccactgaa
    30121 gcgggtgtga atactaacca aagcgaatga ccttcgcaag ggcaatttta tcgcattgta
    30181 tcaactgcgg aaaaaaacag caaaaccgat gtgttcgccg agaaaaaagg cgacaatcgc
    30241 cgccttttta gccagttatc taacgcttta aacggctttt actgcatcgg cgatgcggtg
    30301 tgcaaattca gtcacctgcg cttcgtcttc gccttccacc atcacgcgaa ttaacggttc
    30361 ggtgccggat ttacgcagca acacgcgtcc acggttgccc agcgcagctt caacctctgc
    30421 ggtcacggct ttaactgact catgctcaag cggatcgccg ctacctgcgg tgtaacgtac
    30481 gttaaccaga atctgcggga acattttcat gccgctgcaa aggtcgtgca ggctcatatg
    30541 gttacgtgcc atcgccgcca gcacctgcaa gccagcaacg atgccgtcac cggtagtggt
    30601 tttatccagc aggatcacat gaccggaatt ctctgcaccg atacgccagc ctttctcctg
    30661 cattttttcc agtacgtagc ggtcacccac tttcgcgcgc gcaaatggaa ttcctaactg
    30721 tttcagcgcc agttcaagcc ccatgttgct catcaatgta cccacagcgc caccacgcag
    30781 ctggccctga cgaagacctt cacgcgcgat gatatacatg atctgatcgc catcgacttt
    30841 attgccttca tggtcaacca taatcacgcg atcgccgtcg ccgtcgaagg caataccgag
    30901 atccgctttt tcagccagca cacgagcctg gagcacgcga acgtcggtag cccccacttc
    30961 ggcattgatg tttacaccgt ttggctcaca accgatagcg ataacgttcg cccccagttc
    31021 gcgcagcacg ttcggcgcga tgtgataagt cgcaccgttt gcacaatcca ccacaatctt
    31081 cagttcactg aggctaagtt cgttcgggaa cgtggctttg caaaactcga tatagcgacc
    31141 cgcggcatca acgatacggc tggctttacc cagttctgcc gaatcaacgc agctgatctc
    31201 cttttccatt tccgcttcga tggcctcttc taccgcatcc ggcagtttag tgccgtcgat
    31261 agagaagaat ttaatcccat tatcgtagaa cgggttatgc gatgcagata tcacaattcc
    31321 ggcctctgcg cggaaggtac gcgtcagata agccacggcc ggtgttggca tcgggccagt
    31381 gaagagtgcg gaaaggcccg ctgccgccag acccgcttcc agtgctgact ccagcatata
    31441 gccagaaata cgcgtgtctt taccaataat aatcttacgg gagccgtggc gcgccagcac
    31501 tttacccgcg gcccaaccca gcttaagcac aaaatcaggt gtgatcggcg catcccctac
    31561 acgaccacga atcccatcgg taccgaaata tttacgatta ctcatagcgt ttgttttcct
    31621 ttgcagacag agtggcttcc accacccgca tcgcttctac ggtttctttg acgtcatgaa
    31681 cacgaatgat gtgcgcgcct tgcattgcgg caatgaccgc acaggccaga ctaccgctca
    31741 ggcgctcgga cggccccacg ttcagcagct gcccaatcat cgattttcgt gacataccca
    31801 ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg cgccagtaat gaatagttat
    31861 gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa caatttctct tttgcgatac
    31921 ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg attcacttct gcaaagacat
    31981 cgtcatactt cggagcttcc tgcatggttt ttggatttcc ctgcatatgc atcagacaaa
    32041 ccggtaaacc ggtttctgca gccgcctcca gagcgccagg ttcggaaagg gagcggatat
    32101 cattaataat gtgagcgcca actttcgctg actcacggat gacttctggt ttggatgtat
    32161 cgaccgagat ccagacttcg aagcgttgag caattgcctc aaccacagga ataacacgtt
    32221 gcaactcttc ttcaacgcta acttccgccg cccctgggcg cgtggactcg ccaccaacgt
    32281 caatgatcgt cgcgccagca ttgatcatca gattcgcatg tttcaccgca tctatcagcg
    32341 agttatgcgt gccaccatcc gaaaaggaat caggcgtgac gttgaggatc cccattacgt
    32401 gaggatggct aaggtccagt gaagtaccct gggcaaagag tttcatgatg ttatccctgg
    32461 tatgaattga taagaaaaaa ccccggagca cgccccgggg ttttcggtac aaatacagtc
    32521 atctgatgcg ggaacttact tgtcgcctaa ctgctctgac atggtgttac ccgggttcgg
    32581 cgtacgcggt tcatcaaccg gacgaggagc ctttggacta ccattgtcgc cagaattgtt
    32641 agaagcgcct ggttcttccc agcccgctgg cggacgtaca tcgcgacgtg ccatcaggtc
    32701 atcaatctgc ggtgcgtcga tagtctcata tttcatgaga gcatctttca tcgcatgcag
    32761 aatatccata ttgtcggtca gaagctgacg cgcacgatta tagttacgct caatcagtgc
    32821 tttcacttcc tggtcgatga tacgtgcagt ttcatcggac atatgtttcg ctttcgctac
    32881 gctacggccg aggaatactt caccttcttc ttccgcgtac agcagtggac ccaatttctc
    32941 agagaagccc cactgagtca ccatgttacg tgccaggttg gtcgcaactt taatatcgtt
    33001 ggacgcaccg gtagaaacat gttccggccc gtagatgatc tcttctgcca gacgaccacc
    33061 gtacagcgta gaaatctggc tttccagttt ctgacggctg gcgctgattg cgtcgccctc
    33121 aggcaagaag aaagtcacac ccagcgcacg accgcgtggg ataatcgtca ctttatgcac
    33181 cggatcgtgt tccggcacca ggcgaccgat aatcgcatga cccgcttcgt ggtaagccgt
    33241 tgattctttc tgcgcttccg tcatcaccat ggagcgacgt tccgcaccca tcatgatttt
    33301 gtctttcgct ttctcgaact caaccatcga cacaacgcgt ttgttgccac gagcagcgaa
    33361 cagtgccgct tcgttcacca ggttcgccag gtcagcaccg gagaaaccag gagtaccacg
    33421 ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg cgacgcatgt gaactttcag
    33481 gatctgctca cgaccgcgaa catctggcaa gccgaccaca acctggcggt cgaaacggcc
    33541 aggacgcaac agggccgggt cgagaacgtc cggacggtta gtcgcggcga taacgatgat
    33601 accttcgtta ccttcgaagc catccatctc aaccagcatc tggttcagag tctgttcacg
    33661 ttcatcgtga ccaccgccca gaccagcgcc acgctggcgg cctacggcgt cgatttcatc
    33721 gataaagatg atgcacggtg ccgctttctt cgcctgttcg aacatgtcac gaacacggga
    33781 tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg gagatagtaa agaacggaac
    33841 tttcgcttcg cctgcaatcg ctttcgccag caacgtttta ccggtacccg gaggaccgac
    33901 catcaggacg cctttcggga tcttaccgcc gagtttctgg aagcggctcg gctcgcgcag
    33961 atactctacc agttcagcaa cttcttcttt tgcttcgtcg cagcccgcaa cgtcagcaaa
    34021 ggtcgttttg atctgatctt ccgtcagcat gcgcgctttg ctcttaccaa acgacatggc
    34081 acctttgcca ccgccgccct gcatttgacg catgaagaag atccagacac caatcagcaa
    34141 cagcatcggg aaccaggaga tgaagataga agccagcagg cttggttctt caggcggttc
    34201 accgacaacc ttgacgttct tggtcaacag gttatccagt aatttcggat cctgaaccgg
    34261 aatgtaagtg gtataacggt tactatcttt cttggtaacg ttgatttcac gtccgttgat
    34321 acgcgcttca cgaacctggt cgttattgac ctcttgtagg aaggtagagt aatccacctt
    34381 acggccatta gactcgctgg gcccaaagct ctggaatact gacatcagca caacggcaat
    34441 gaccagccag agtattaggt ttttcgccat gtcactcaag ggattaacct cttattacaa
    34501 ctgtgttaaa aacagcgtca ggatactcta tatccagcat ctttcaaact ttcgtctgaa
    34561 atctcccggt tagggtttac gcccggtcgc tacaatatac acttctcgcg aacgtgcacg
    34621 agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg gagcgaattt cccttagata
    34681 ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa ctgccacctg gcgctaatac
    34741 atcacgacac atttctagcg ccagttccac cagatacatg gcacggggga tatccaccgc
    34801 cggtgttccg ctcatgtttg gtgccatatc ggacatgaca acctggactt tgctgtcgcc
    34861 aacgcgctcc agcagtgctt tcatcaccag ttcatcacga aaatcgccct gaagaaagtc
    34921 cacaccaacg ataggatcca taggtaaaag atcgcaagcg atgatgcggc ctttgccgcc
    34981 aatttgggtg accacatatt gtgaccaacc acccggagca gcaccaaggt cgacaaccgt
    35041 cattcccggt ttaaagagtt tgtcactttg ctgtatttca tcaagtttaa accaggcacg
    35101 ggaacgtaat ccctttttct gtgcctgttg aacatattta tcgctaaagt gttcctgaag
    35161 ccagcggctg gagctggcag aacgcttctt acctgtcatt taactttccc atggggataa
    35221 ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt ggtgatatat gggagatggc
    35281 ggtagaatga cccgttttca atcccaacgt aagcaaaaaa tacgatgaat ctgagtacta
    35341 aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa gccagttgtt ctgcttggca
    35401 gtaatggttt gaccgaaggg gtgctggccg agattgaaca agcgttagag caccatgaac
    35461 tcatcaaggt gaaaatcgcc accgaagatc gcgaaacaaa aaccttgatc gtggaagcta
    35521 tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg taaaacgctg gtgctttatc
    35581 gcccaactaa aaaacgtaaa atctcgctgc cacgctaaga ttatcctaaa gttacacaca
    35641 tttgctgtgt aaaacgaggg gttttccgca ggcaggagag caaaatgcca cgctctgttc
    35701 gttgataaaa ggccgcagag cggccttttt cctttcttta caatacatca acatcttgag
    35761 tattgggtaa ttcttacagg tattccacct taattacttc aaattctact tcgccgcccg
    35821 gcgttttgat gaccacaaca tcatcttctt ctttgccgat caggccacga gcaataggcg
    35881 agtttacaga aatcaggttt tgtttaaagt cagcttcgtc atcgccaacg atgcgataag
    35941 tctgttcttc gtcagaatcc agattcagca ccgttacggt agcaccaaaa ataacgcgcc
    36001 cattgttggg cattttggtg acatcaatca cctgcgcgtt tgacagcttg gcttcgatgt
    36061 ctttgatacg gccttcgcag aagccctgct gttcacgagc tgcgtggtat tcggcgtttt
    36121 ctttcaggtc gccatgctca cgcgcttccg cgatagcagc aatgatttca ggacggcgca
    36181 cagatttcag aaaatccagc tcttcgcgta atttttcagc gccgcgtaag gtcatcggaa
    36241 tagcttgcat ttgttatacc tcttgaatat tcctgatggg gcaagtcttc acccaccccg
    36301 gctgttcggc ctgcccggca taactcctca ccgggaccag aagcaaaaaa ataccgaccc
    36361 gggtacaagt cccaggtcag ctacaattca cattttgatg gtcattttac cctgaagttc
    36421 ccgaagggtc atcgtttact ttatagggcg ttgcgccgta gtatgacggc tcgattccag
    36481 gttgttagcg cgagattatg cgattttcca gatttatcat cggattgacc agctgtatag
    36541 cgttcagtgt tcaggccgcg aatgttgatg agtacgttac tcaactcccc gcaggtgcca
    36601 accttgccct gatggtgcaa aaagtcggtg cgtcggcccc cgctattgat taccacagtc
    36661 agcagatggc gctgcccgcc agtacccaga aagtgattac tgcgctggcg gcgttgattc
    36721 aactcggccc cgattttcgt ttcaccacga cgcttgaaac caaaggcaat gtggaaaacg
    36781 gcgtacttaa gggtgactta gtggcgcgat ttggtgccga tccgacgtta aaacgtcagg
    36841 atattcgcaa tatggtcgcg actttgaaaa aatctggcgt caaccaaatc gatggcaatg
    36901 tgttgataga tacctccatt ttcgccagcc acgataaagc ccccggctgg ccatggaatg
    36961 acatgacaca atgctttagc gctccgcctg ctgccgccat agttgaccgc aactgtttct
    37021 ccgtctcgct ctacagtgcc ccaaagcctg gtgatatggc ttttatacgc gtggcatctt
    37081 attaccccgt tacgatgttc agccaggtac gcaccctccc ccgtggttct gccgaagcgc
    37141 aatactgcga actggatgtg gtgccaggcg acctgaaccg ctttacgctg acgggatgcc
    37201 tgccacaacg ttctgagccg ctcccgttgg cttttggcgt gcaggatgga gccagctatg
    37261 ccggtgcaat tctgaaagat gagttaaaac aggcgggtat cacctggagc ggaacactgc
    37321 tgcgccagac tcaggttaac gaacctggaa cggtagttgc cagtaaacag tcggccccgc
    37381 tgcacgatct gcttaagatt atgctgaaaa agtcggacaa catgatcgcc gatacggttt
    37441 tccgcatgat cggccatgcg cgcttcaatg tgcctggaac atggcgggct gggtcggacg
    37501 ccgtgcgtca gatcctgcgc cagcaagccg gtgtcgatat tggtaacacc attattgccg
    37561 atggttcagg gctttcgcgg cataacctga ttgcccccgc caccatgatg caggtgctgc
    37621 aatacattgc ccaacacgac aatgaactta actttatctc catgctgccg cttgcgggct
    37681 atgacggctc tttgcagtac cgtgcaggtc tgcatcaggc gggcgtggat ggcaaagtct
    37741 cagcgaaaac cggttcgttg cagggggtat ataacctggc gggattcatt accacagcga
    37801 gcgggcaacg aatggcattt gtacaatatc tttctggcta tgcagtaaaa cccgcggatc
    37861 agcgtaatcg ccgtattccg ttagtgcgtt ttgaaagccg tttgtacaaa gatatttatc
    37921 agaacaatta gtcaaaaaga aaccccggcg catggccggg gtttcagatt attgacaaag
    37981 tgcgctttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa aatcgtgcaa
    38041 attcaatata ttgcaatcat cttgtaggcc tgataagtgt agcgcatcag gcttatttgg
    38101 cattggtcat tagtgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt
    38161 cccagtcgtc atcccagtct tcatcatcct cttcagcaat ctcttcaagc tgctgacggt
    38221 gataatcatc ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga
    38281 ccgggttttc aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccgc
    38341 tcgccgcaga gatcagataa tatttatctt cccagcccag cgcttcagcg atcgctttcg
    38401 ctttctcttc ggcttctgcc ttatccagca gatcgatctt gttgaagact aaccaacgcg
    38461 gtttcgccgc cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt
    38521 caaccggatc ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac
    38581 gttccaggtg cttcaggaag cgaatgccca gacctgcgcc ttccgcagcg ccttcaatca
    38641 gtcctggaat atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac
    38701 ttggcaccag agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcac
    38761 gaataaaggt cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca
    38821 tcagctccag cagcagctca cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg
    38881 tacggttaac tgacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa
    38941 ccagcagacg ctgaccgtgt ttagtcatat cacccatggt ttcaccagta ccctggtcga
    39001 ttacacgcgt acctaccggc actttaatcg tcacgtcttt accgcgctta ccggtacaat
    39061 cgcggcttgc gccgttctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga
    39121 taagcgtgtt caggttctcg tcggcttcca tccatacgtc accaccatca ccgccgtcgc
    39181 cgccatccgg gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac
    39241 cgccatcacc tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg
    39301 taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc
    39361 aaatacgatg accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat
    39421 aacctaaaag gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat
    39481 ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgagctgcct
    39541 gccagcgagc catcgcttcc gccaaggcgc catatcctac caaggtattc agtccgcaaa
    39601 aaattaaaca tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag
    39661 ggaagagcgc gattgtacat aaagtgtaca gtaaaaacag gatctgcggt gaggccagcc
    39721 gacgcaataa aaccttttgc gccacgccat aactcaccca aaccgtcgcc gcaccgaccc
    39781 caaagataac tccccaggtg taatcggtga gctttgtaaa tatctcgacc agactggtgt
    39841 taaaaaacat caccagaccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca
    39901 ttttctcttt caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa
    39961 tcacctgcga agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc
    40021 caaacagccc ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc
    40081 gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc aaggccaata ctcgccatca
    40141 agaaacggta aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcga
    40201 ttggcaacgc cccccagcaa attgctgtgg taagcgccaa aagaatgcca atgcctgcct
    40261 gctgcttcat accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca
    40321 tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta
    40381 ccgggacgcg aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt
    40441 taacttcgaa tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac
    40501 ctacgttagc gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca
    40561 gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt
    40621 tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc
    40681 gctgatgcca gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata
    40741 gtgtttacga cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac
    40801 ttcagctttg attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt
    40861 tgcgatcatc agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc
    40921 caggcgaacg gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc
    40981 gtacatataa aactccgctt ccgcgcacac cttttcaatg attcaaagtg cgctataaat
    41041 attcacaata gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa
    41101 tacacgaaga aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta
    41161 caattaccct atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc
    41221 ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa
    41281 gatatggcgg gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccaactgatc
    41341 aatcagttag gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct
    41401 gtactggctg cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg
    41461 atcgagttta tccacacggc gactctgcta cacgacgacg ttgtggatga atcagatatg
    41521 cgcaggggta aagctaccgc caacgccgca tttggcaatg ccgccagcgt gctggtaggc
    41581 gattttattt atacccgcgc gttccagatg atgaccagcc tcggttcact caaagtgctg
    41641 gaagtcatgt cagaagctgt aaacgtcatc gcagaaggtg aagttctgca actgatgaac
    41701 gttaacgatc cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg
    41761 cgtctgtttg aggctgccgc gcagtgttcc gggattctgg ctggctgtac gccggaggag
    41821 gagaaaggcc tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat
    41881 ttactcgatt acaatgccga tggcgaacag ttaggtaaaa acgtcggcga cgatctgaac
    41941 gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca
    42001 cagatgatcc gtaccgccat cgaacaaggt aacggtcgcc atcttctgga accggttctg
    42061 gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc agcgcgccga ggaagaagca
    42121 gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc
    42181 ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc ctcccttcat cccgcgcagt
    42241 gcgcgggatg attccagtaa attccataaa aacactaatt cagcgctata acacctgaaa
    42301 aaaagggcga tgaatcttta tatattctga atattcacac tctttacagg aactttttag
    42361 cgcaataggc catcaggagt atagtgatgc ccgacagaag aagtgttctg aatgaaagcg
    42421 actaacttaa ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca
    42481 tcattgcagg tttgcgcaag aaaggaacgt caatggcggc ggaatctcgc agaaatggtt
    42541 tgagttcctc aacgctggca aacgcattat cgcgcccatg gccgaaagga gagatgatta
    42601 ttgcgaaagc cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc
    42661 agacccatga gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat
    42721 gagtggtctg gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt
    42781 tcacacgctc aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac
    42841 gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca
    42901 ccaggcttgc tgatgcacgc agatcggttg ccataacctg tgcaccagaa agtttttcaa
    42961 caccatgaca aataacggta ttgctttcga tttcggcgtg cgcgcccata cggctcagct
    43021 ctggcacatg cataaagcgg ttttcaaaga ccgtttcggt gatgaacccg gtcccttcag
    43081 ccaccaggtt caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg
    43141 cggtacgtac gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt
    43201 cgccgacttc gatgtccgct ccagcatcac gcagtttcgc cagcacggcg tccagagtat
    43261 ctggctgcgc gttacggcag ataattttgc cacgagagat cgccgccgcc accaggaaag
    43321 taccggtttc gatacgatcc ggcagcacgc gatagacacc gccgcctaaa cgttccacac
    43381 cttcgatgac gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga
    43441 agttcgcggt atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtgcctt
    43501 ccgccagggt tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca
    43561 cgatatgtgc gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt
    43621 tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgagcaccga
    43681 tcgtacaacc gccaggtagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca
    43741 gcgcccagat agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa
    43801 cgtcgcgggc atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta
    43861 gcagtttcat tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt
    43921 cttccgccag tagtgcggca aaaagaatag gcagagcagc atttttagcg ccggaaattg
    43981 tgacttcgcc ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc
    44041 tcagttaaca attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt
    44101 tgcgatcgcg cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt
    44161 tatccgcaat atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca
    44221 taccgtcaaa caactcaccc acggcaataa cctgaaagtg actgccatcg ccggaaacgt
    44281 ggacttcctg gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg
    44341 gatcttcaat catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat
    44401 aaataagcaa aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc
    44461 ggacaggcgg ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag
    44521 gcaaattata caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt
    44581 tgttgccctg ctttttcgcc agatcgataa gatggagcag cagtgccagt ccccccgtat
    44641 ccacgcggga cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca
    44701 tttcccaaag cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac
    44761 ccgtctgcat ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg
    44821 ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt
    44881 tccccactcg ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc
    44941 ttgccagttt cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg
    45001 gccattcggg tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg
    45061 ttctggcgca atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg
    45121 cttcaggtac tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt
    45181 gtaatactgg cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc
    45241 ctgatcaaca atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt
    45301 caggcgatcg aacgttttct gcgccgcctc gtccatcagc ttataaggat tggtctggtc
    45361 tgccgcggtt gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt
    45421 aaacatgcgt cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt
    45481 attacctggc gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc
    45541 gtaaaggaac tgaccaatga gatcttccag caccatcgca gacttggtgt cctgaattgt
    45601 atcgccatcc ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc
    45661 cagatattgt tctcccagca ggccggaagt acgaatgctc agcgaactgg tatcaggaat
    45721 gtggttataa cgttgttcaa tttccagcgt tacgcgcggc agataggttt tcgggtccag
    45781 cgtaatatcc gccacccggc ccacaacaac accaccaatg ctgaccggag agcgcgcttt
    45841 caggccgcca atgttatcga acgtcgcata aagcgtgtag gtcgattcag tacgtatgga
    45901 cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa
    45961 gatacccacc caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg
    46021 cggtcagcac aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg
    46081 ttgcccggct aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc
    46141 acgtcaccgt gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc
    46201 agtcgacggc attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga
    46261 cgccgaccag cgatccgccc cagatcccca cggcgacgaa gataaccgtc aacagtggta
    46321 atgaaataac cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca
    46381 tcatctccat actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttaacg
    46441 ccgaaccagc acgcccggca aacaacagcg cggcaaccac cggccccagt tcacgcagta
    46501 gcgataacgc caccagcata cccagactgg tttccgcgct ataagtggtc agaaccagat
    46561 aaccttgcag ccccaacacc attccgatga acacgccaga aactacaata atcagcatcg
    46621 acaggacgcc gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg
    46681 gtttgccgac cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt
    46741 taatcccttt atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct
    46801 ggtaaaagat cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct
    46861 atcccgtcca gaaactgacg tacgcgcgga tcaggattcg tctgcaacgc ctgggcactg
    46921 ccatgagcga caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact
    46981 tccggcacat cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa
    47041 atcagcttca ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac
    47101 atgatgagat ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca
    47161 ccggaaagtt cagaaggcat tagtttagcc gctccacgca gccccacggc ctccagcttc
    47221 atcatcaccg tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag
    47281 gcgacgttgt caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc
    47341 atccgtttac gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg
    47401 aaaaggatct caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt
    47461 ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc ctcgcggcac ggtcagggaa
    47521 atattatcga agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa
    47581 ttcgccacag actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg
    47641 ctcaatttaa ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag
    47701 ctggcatttg ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt
    47761 ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa ggacgttaga tgcttttagc
    47821 tacggcactg ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt
    47881 tgccgcgtct attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt
    47941 ggtcagtatt ggtacatcgt taccagaaat catcgtctcg cttgccgcgt ctctgcacga
    48001 acaacgcgat ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat
    48061 cctcggtctg gctgcgctgg ttcgtccttt taccgtccat tccgatgttc tgcgccgtga
    48121 attaccctta atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact
    48181 tagtcgaagc gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt
    48241 taaacttgca cgtcagggga ctgacagcct gaccagagag cagcttgcag agctgccgcg
    48301 tgacggcgga ttgcccgtcg cgtttttatg gctcggcatt gcgcttatca tcatgccagt
    48361 ggccacgcgg atggtggttg ataacgccac ggtgctggcg aattactttg ccatcagcga
    48421 gttgacgatg ggtctgacgg caattgctat cggaaccagt ctgccggaac tggcaaccgc
    48481 aatagcgggg gttcgcaaag gtgaaaacga cattgctgtc ggaaatatca ttggcgcaaa
    48541 catttttaat attgtcatcg tgttgggttt acccgcgctg ataacgccag gagagattga
    48601 tccactggcg tacagtcgtg actacagcgt gatgttgctg gtgagcatta tttttgcgtt
    48661 gctgtgctgg cggcgctccc cgcaaccggg ccgtggtgta ggggtattat taactggcgg
    48721 atttatcata tggctggcga tgttgtactg gttatcgcca atactcgttg aataactgga
    48781 aacgcattat gtcgcacgta gagttacaac cgggttttga ctttcagcaa gcaggtaaag
    48841 aagtcctggc gattgaacgt gaatgcctgg cggagcttga tcaatacatc aatcagaatt
    48901 tcacgcttgc ctgtgaaaag atgttctggt gtaaagggaa agttgtcgtc atggggatgg
    48961 ggaaatcggg gcacatcggg cgcaaaatgg ccgcaacgtt tgccagcacc ggtacacctt
    49021 catttttcgt ccatcctagt gaagccgcgc atggtgattt aggcatggtc accccacagg
    49081 atgtggtgat tgctatctct aactcaggtg aatccagcga aatcacggcc ttaattccag
    49141 tgcttaagcg tcttcacgta ccgttaatct gcatcaccgg tcgcccggag agcagcatgg
    49201 cgcgcgccgc agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt ccgttagggc
    49261 tggcaccgac cagcagcacc accgccacgc tggttatggg cgatgccctc gctgtcgcgc
    49321 tgttaaaagc acgcggcttt actgctgaag attttgcgct ctcacaccca ggcggcgcac
    49381 tgggtcgtaa acttctgctg cgcgtaaacg atattatgca tacgggcgat gagatcccgc
    49441 atgttaagaa aacggccagt ctgcgtgacg cattgctgga agttacccgc aaaaatcttg
    49501 gtatgactgt catttgcgat gacaatatga tgattgaagg catctttacc gacggtgatt
    49561 tacgccgtgt cttcgatatg ggcgtggatg ttcgtcagtt aagtattgcc gatgtgatga
    49621 cgccgggggg aatacgtgtg cgccctggca ttctggccgt tgaggcactg aacttaatgc
    49681 agtcccgcca tatcacctcc gtgatggttg ccgatggcga ccatttactc ggtgtgttac
    49741 atatgcatga tttactgcgt gcaggcgtag tgtaaagatt caaggataaa caacaatgag
    49801 caaagcaggt gcgtcgcttg cgacctgtta cggccctgtc agcgccgacg ttatggcaaa
    49861 agcagagaac attcgtctgc tgatcctcga cgttgatggc gtactgtcag atggcctgat
    49921 ttatatgggc aataacggcg aagagctgaa agcgttcaat gttcgtgacg gttatggcat
    49981 tcgttgtgcg ctcacctctg atattgaagt cgctatcatt accgggcgaa aggctaaact
    50041 ggtagaagat cgttgtgcca cattggggat cactcacttg tatcaggggc agtcaaacaa
    50101 actgatcgcc tttagcgatt tgctggaaaa attggcgatt gccccggaaa atgtggctta
    50161 tgttggcgat gatctcatcg actgcccggt aatggaaaaa gtgggtttaa gcgtcgccgt
    50221 ggccgatgcc catccactgt tgatcccgcg cgccgattac gtgacgcgca ttgctggcgg
    50281 tcgtggcgca gtgcgcgaag tttgcgactt attactcctg gcgcagggca aactggatga
    50341 agccaaaggg caatcgatat gagtaaagcc agacgttggg ttatcattgt gctatcactg
    50401 gcggttctgg tgatgatcgg cattaatatg gccgaaaaag acgataccgc ccaggtggtc
    50461 gtcaacaaca atgatcccac ctataaaagc gagcatacgg acacgctcgt ctataaccca
    50521 gaaggggcac taagctatcg attgattgct caacacgttg aatattattc cgatcaggcc
    50581 gtttcgtggt ttacgcagcc ggtacttacc acgtttgata aggataaaat cccgacatgg
    50641 tccgtaaaag cagataaagc caagctgacc aatgaccgga tgctctattt atatggacac
    50701 gttgaagtca acgcacttgt gccagactct caacttcgca gaatcacgac ggataacgcg
    50761 cagatcaatc tggtgacgca ggatgttacc tctgaagacc tcgtcacgtt atacggaaca
    50821 acatttaact ccagcggtct gaaaatgcgc ggcaacttac gcagcaagaa cgccgagctg
    50881 attgaaaagg ttagaacatc ctatgaaatt caaaacaaac aaactcagcc ttaatcttgt
    50941 gcttgccagc tcacttctgg ccgccagcat tccggcattt gccgtaaccg gagacactga
    51001 tcagccgatc cacattgaat cggaccagca atctcttgat atgcaaggca acgtggttac
    51061 ctttaccggt aatgtcatcg tcacccaggg caccatcaaa attaatgccg acaaagtggt
    51121 cgttacccgt ccgggcggcg aacaaggtaa agaagtgatt gacggatacg gtaaaccggc
    51181 aacgttctac cagatgcagg acaacggtaa acccgttgaa ggtcacgctt cccagatgca
    51241 ctacgaactg gcaaaagatt ttgtcgttct gacgggtaat gcttatctgc agcaggtcga
    51301 tagcaacatt aagggcgata agatcactta cctggtgaaa gagcagaaaa tgcaggcttt
    51361 cagcgacaaa ggcaagcgcg taaccaccgt tctggtgccg tcgcagctgc aggacaaaaa
    51421 caacaaaggc cagaccccgg cacagaagaa gggtaattaa ttcgttatgg caacattaac
    51481 tgcaaagaac cttgcaaaag cctataaagg ccgtcgcgtg gtagaagacg tcagcctgac
    51541 cgtcaactcc ggggaaattg tcggtctgct ggggccaaac ggtgccggta agaccaccac
    51601 tttctacatg gttgtaggca ttgtgccgcg cgatgcgggc aacatcatta ttgatgatga
    51661 cgatatcagt ctgctgcctc tgcatgcacg cgcgcgccgc ggtatcggct atctgccaca
    51721 ggaagcctcc attttccgtc gcctcagcgt ttacgataac ctgatggcgg tactgcaaat
    51781 tcgtgacgac ttgtctgctg aacaacgtga agaccgcgcg aacgagctga tggaagagtt
    51841 tcacattgag cacctgcgtg acagcatggg gcagtcactc tccgggggtg aacgtcgccg
    51901 tgtagaaatt gcccgcgcac tggctgcgaa tccgaaattt attctgctcg acgaaccgtt
    51961 tgccggggtt gacccgatct cggttatcga tattaaacgc atcattgaac acctgcgcga
    52021 cagcggcctg ggcgtgctga tcactgacca caacgtgcgt gaaacactgg cggtttgtga
    52081 acgcgcttat atcgtcagtc aggggcattt gatcgcccac ggcacgccta cagaaatctt
    52141 acaagacgaa cacgttaagc gtgtatacct tggggaagac ttcagactct gatagggtag
    52201 aagtttgcga cgttttagca ggagagtacg attctgaaca tgaagcaagg tttgcaactc
    52261 aggcttagcc aacaactggc gatgacgcca cagctccaac aggcaattcg tctgttgcag
    52321 ttgtcgacgc tggaacttca gcaggagcta cagcaggcgc tggagagtaa tccgctgctt
    52381 gagcaaatcg acactcatga agaaatcgac acccgcgaaa cgcaagacag tgaaacgctg
    52441 gacaccgccg acgcgctcga acaaaaagag atgccggaag agctgccgct cgatgccagt
    52501 tgggacacca tttacaccgc tggtacacca tccggcacca gcggtgacta cattgacgac
    52561 gagctgccgg tctaccaggg cgaaacgacg cagaccttgc aggattacct gatgtggcag
    52621 gtcgagctga caccgttttc cgacactgac cgcgctattg ctacctctat cgtcgatgcc
    52681 gttgatgaaa ccggttatct gactgtcccg ctggaagata ttctcgaaag tatgggcgat
    52741 gaagagatcg acatcgacga ggttgaagcc gtccttaagc ggatccaacg gtttgatccg
    52801 gtcggtgtag cggcaaaaga tctgcgtgac tgcctgctaa tccaactctc ccaattcgat
    52861 aagaccacgc cgtggctgga agaggccaga ttgatcatta gcgatcatct cgatctgtta
    52921 gccaatcacg acttccgcac tttaatgcgc gtcacgcgtc tgaaagaaga tgtgctgaaa
    52981 gaagccgtca atctgatcca gtcgctcgat ccgcgccccg ggcagtcgat ccagactggc
    53041 gaacctgagt atgtcattcc agatgtgctg gtgcgtaagc ataacggtca ctggacggta
    53101 gaactcaaca gtgacagcat tccgcgtctg caaatcaacc agcactacgc ctcgatgtgc
    53161 aataacgcgc gcaacgatgg tgacagccag tttatccgca gcaatctgca ggatgccaaa
    53221 tggttgatca agagtctgga aagccgtaac gatacgctac tgcgcgtgag tcgctgtatc
    53281 gttgaacagc agcaagcctt ctttgagcaa ggtgaagaat atatgaaacc gatggtactg
    53341 gccgatatcg cccaggctgt cgaaatgcat gaatcgacga tatctcgcgt gaccacgcaa
    53401 aaatacctgc atagtccacg aggcattttt gaactgaagt atttcttttc cagtcacgtc
    53461 aataccgagg gcggcggcga agcttcctcc acggcgattc gtgcactggt gaagaaatta
    53521 atcgcggcgg aaaacccagc gaaaccgttg agcgacagca agttaacctc tttgctgtcg
    53581 gaacaaggta tcatggtggc acgccgcact gttgcgaagt accgagagtc tttatccatt
    53641 ccgccgtcaa accagcgtaa acagctcgtt tgacccaacc gataaggaag acactatgca
    53701 gctcaacatt accggaaata acgtcgagat caccgaggca ctgcgcgaat ttgttacagc
    53761 taaatttgcc aaacttgagc aatattttga ccgaatcaac caggtctatg ttgttctgaa
    53821 agtggagaaa gtcacccaca cctcagatgc aacactgcat gtaaacggtg gtgaaattca
    53881 tgccagcgcg gaaggtcagg acatgtacgc tgccattgat ggcttaattg ataagctggc
    53941 acgccagttg accaaacata aagataaact gaaacaacac taattgtccg ggcaattagc
    54001 atgtgcatgg cggcctgttg tgcggcacaa cgggccattt gtacggttaa tgctccgagc
    54061 ctgttccact gtttgagtgg gcaggttctt aggtgaaatt atgacaaata atgatacaac
    54121 tctacagctt agcagtgttc ttaacaggga atgtacgcga agccgcgtcc actgtcagag
    54181 caaaaaacgc gccctggaaa tcatcagcga gctggcggcg aaacaactta gcctaccgcc
    54241 tcaggtggtt tttgaagcta tcctgacgcg ggaaaaaatg ggcagtaccg gtatcggcaa
    54301 tggtattgcc attccgcatg gcaaacttga agaagatact ctgcgtgccg ttggcgtttt
    54361 cgttcagctc gaaacgccta tagctttcga tgccatcgac aaccaaccgg tggacttact
    54421 ttttgccctg ctggtgccgg cagaccaaac taaaacgcac ctgcatactc tgtcgctggt
    54481 ggcgaaacgt ctggcggaca aaaccatctg ccgccgttta cgcgcagccc agagcgatga
    54541 agagctgtat caaatcatta cggataccga aggtactccg gatgaagcgt agttattcgg
    54601 taatgtctct tttagacgtt gtgaggagaa acagtacatg gtactgatga tcgtcagcgg
    54661 acgttcaggt tcaggtaaat ctgtcgccct gcgtgcgctg gaagatatgg gtttttactg
    54721 cgtggataac cttcccgtag tgttgttacc cgatctggct cgaactctgg ccgatcgcga
    54781 gatttctgcc gccgtcagca ttgatgttcg taatatgccg gagtcaccag aaatattcga
    54841 acaggcgatg agtaacctgc ctgacgcttt ctcaccgcaa ctactgttcc tggatgccga
    54901 ccgtaatacc ttaattcgtc gttacagtga cacgcgccga ctgcatccgc tttccagcaa
    54961 aaacctgtcg ctggaaagtg ctatcgacaa agaaagcgat ttgctggagc ctctgcgttc
    55021 gcgagcggat ctgattgtcg atacctcaga aatgtccgtt cacgagctgg ctgaaatgct
    55081 gcgtacccgt ctgctgggta aacgtgaacg cgaactgacc atggtctttg agtctttcgg
    55141 cttcaaacac ggtatcccta tcgatgcaga ttacgtcttt gacgtgcgct tcttgccgaa
    55201 cccgcactgg gatccgaaac tgcgtccaat gacaggtctt gataaacctg tcgccgcgtt
    55261 cctcgaccgc cacacagaag tacacaattt tatctaccag acgcgaagct atcttgagct
    55321 atggttacct atgctggaaa ccaacaaccg tagctacctg acggtcgcca ttggttgtac
    55381 cggcgggaag caccgttcgg tgtatattgc agagcaactg gcagactact tccgctcgcg
    55441 cggtaaaaac gtccagtcac gccatcgtac gctggaaaaa cgtaaaccat gaccgtcaag
    55501 caaactgttg aaatcacaaa caagctgggc atgcatgccc ggcctgcaat gaagctgttt
    55561 gaattaatgc agggttttga cgctgaagtg ctgttacgta atgacgaagg caccgaggct
    55621 gaagccaaca gcgttattgc gctgctgatg ttggattctg ccaaaggacg gcagattgaa
    55681 gttgaagcga ccggtccaca ggaagaggaa gcactggccg ccgttatcgc cctctttaat
    55741 tctggttttg atgaagatta atcttcatca ctttgacata caactccctt caagctcccc
    55801 cgacaataag aaaatcacgt actgaaatcg ttctcaatca acgtcatttg tacattttgt
    55861 gcgcttttca ctttcagaag aaccttaaga aaaccttaag aggcattgtt taggttttgt
    55921 ttaagttaat cgaccatact ggagatcgtc agaaaatatt tccaggagat ggcatgattc
    55981 gcttatctga acaaagtccc ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg
    56041 aagatgccca acgctgtatc aagattgtct accatcgtgg cgatggtggc gataaagaga
    56101 tccgccgtga gttaaagtac tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa
    56161 taccgcgcta tcacggtacc gttgagacgg attgcggaac cggttatgtc tacgatgtga
    56221 ttgccgattt tgacggcaag ccttccatta cgctgaccga atttgctgaa caatgccgtt
    56281 acgaagaaga cattgcacaa ctgcgccagc tactgaaaca gctgaagcgt tatttgcagg
    56341 ataaccgtat cgtgacgatg tcgctgaagc cgcagaatat cctctgtcac cgcatcagtg
    56401 agtcagaggt tatcccggtc gtctgcgaca acattggtga aagcacattg attccactgg
    56461 cgacctggtc gaagtggtgt tgtctgcgta agcaagagcg gttatggaaa cgatttatcg
    56521 cccaaccggc gctggctatt gccctgcaga aagatctgca acctcgtgag agcaaaacgc
    56581 tggccctcac ttcgcgcgaa gcttaatcca gctggtgttg ttgcataaac ggctcaccgc
    56641 ctaactgata catctgccgt aaaatccacg cctgacggct acgcacgtag ccgtcaggcg
    56701 tggagacttt gaaacgaagt ggattaggta atacagctgc cagtaatgca gcttccgacc
    56761 gggtaagttt gctcgcgggt ttgtggaaat aacgttgtgc cgcagcttcg acgccaaaca
    56821 caccgtcgcc aaattcggcg atattcaggt aaaccgtcag gatacgcttt ttgctccaga
    56881 ctgtttctat ccccagcgtt aatccagctt ccagcccttt tcgtacccag ctacgcccat
    56941 cccataaaaa gagatttttg gccgtctgct gagaaatcgt tgaagcaccg cgaatacggt
    57001 tttcattgcg ctcgttgtgc gccagggctt tctcaatgga agcgacatca aagccccagt
    57061 gctcaggaaa tttctgatct tctgcggcaa tcaccgccag tcccatccac ggcgagattt
    57121 gatccatact gacccagtca gaatgtgcca cgtaacgaaa attgccatgc agccaggcgc
    57181 tgacctgtcg ctcgaccatt accgctgaga agggaacagg cgcaacgcta aacaacgcga
    57241 tgcccccgcc ccagaaaacg gcgaggacaa ccattaaccg caaaagaaaa cggcgaacga
    57301 aactaaacac cgttaagcgg cttttactca ttcagccaga accagcacgc gggaaaccag
    57361 cttatcaatg ccgctcgccg cttctgcaat gttctgcgcc agcatatatg ctggggtggt
    57421 gacaatctta ttgtcttcat caaccacgat atcatcgaca ggacacggca catgctccgc
    57481 gcccatctct tccagtactt ctgcggtatc gatatcagta ccgatggtca aacgcagcgg
    57541 gaaatcgaaa attttcggca gcatcgccgg ggcaatacac ataaaaccaa gcggttttcc
    57601 ggcctgatgc atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc attcgctacc
    57661 aagactggca aaattgctta aattcttcgc cgcgccaacc ccccccggca caatcaacgc
    57721 atccagttca gcggcatcgg cctgggccag aggacggatt tcaccgcgcg ttattcgtgc
    57781 cgcttcaatc agcacattgc gcgtttccgt catcgcttcg ccagttaaat ggttgataac
    57841 atcaacctgc tgcttatccg gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc
    57901 taacaacgtc aacaccgctt catgaatttc agaaccgtca tagacgccgc atccgctcag
    57961 aattacgcca attttcttca ttgtgatcat cctttcgcaa ttgactgaaa cacattaatt
    58021 tttttaataa aaatggtacg catcacacat ttaactgatt catgtaacaa atcatttaag
    58081 ttttgctatc ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt aactcatcat
    58141 aataatttac ggcgcagcca agatttccct ggtgttggcg cagtattcgc gcaccccggt
    58201 ctagccgggg tcatttttta gtggcttttg ccacccacgc tttcagcact tctacgtcgt
    58261 gacgccactc ttctttcatc tcttcaatcc attcaccgac gttatcttcc caggcgggaa
    58321 ggtcaggaga ctgaatttgc tgacccagct gttgcagatg gcgtaacccc actgaccccg
    58381 ccgcaccttt aattttatgt ccttcctcaa caatgccttt tttatcctgc gccgtcagat
    58441 tcgactccag cacgctgaca tagcccggca tcatcttctc aaacaccgct aacccgtcgg
    58501 tgatcagctt cggtcctaca agttcgagat actgttccag catgggaata tcgagcaatg
    58561 cttctgattt actgttctct tctgtcgtca ccgtactctt ctcatcatcc tgggtatccc
    58621 agaatttctt gatcatcgcg gttagcgccg gaaccgaaag cggcttactc agcacatcat
    58681 ccattccagc attgaggtac tcttgtttgt ctttcagcac gttagcggtt aaggccacca
    58741 gcggcggtaa atcctcgcgc ggataacgct tcgtcagttc acgagagata tccagcccgg
    58801 tcatatctgg caactgaata tccagcaaca ccaggtcgta ttcgcccggt ttaaacatct
    58861 ccagcgccgc cttgccggtc atggcgacat caacgctgtt acctaatttt tccagcacag
    58921 aacgcgcgac aatcacgttc agttcaatgt cttccaccag cagcacattc agcgccggta
    58981 aaggcatatc gtcttcatca aacgcatcat cgacctcttc tgccaccgac ggtgcgtgga
    59041 tcgtcaacgt aaaggttgaa cctttgccct gttcgctggt aaccgtaata tcgccgccca
    59101 tatttttcgc cagacgacga gaaacggcca gaccaatacc ggtgccggtg gcaggtttac
    59161 cgccatgact gtctttcacc tggtaataca tggcgaagat tttatccagc tcatcctgcg
    59221 gaatgccgat accagaatct tccacttcaa aatgcagcat atcgccttca tcgtagcgca
    59281 cgcgcacggt aacctggcct tgctgggtga atttgacggc gttactgatg aggttccaca
    59341 ggatctgccg taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc agcgtaggct
    59401 ccaggttaaa gcgcaatcct ttttgttgcg cctgcaatgc ggagagattt tccagatcgg
    59461 caaggaagct ggtgaaatca accggctgat tatcaagctg gaccttgcgc cgttccatct
    59521 tatccatgtc gataatatcg ttaaagatat tccccagcgt gacggccgaa acatggatag
    59581 ttttgagata tttttcctgc tcggcggtga gttcggtatc cagcagaatg cggctcaggc
    59641 cgacgatacc gttcagcggt gtacgcaatt cgtgactgat ggtggaaata aacgtcgttt
    59701 tgtcgcggct ggcgcgttca agcgcatcct gataccgctt acgctcggta atgtcgcgac
    59761 caaagcccat caaaccgtga cgtttaccca cgcggtcgta gtacggcact ttacggattt
    59821 caaagcaggc tttgcgcccg tccgggtaat ccagccactg ttcataggtc agtgacacat
    59881 tatgacggaa cactttttca tcggtttcaa tgacttttgc ggcggcttcc ggtgagtaaa
    59941 catcagcagg tttcagatga accagttgtt tttcgctttt tccggtcagc agctccatcg
    60001 cgcggttaca gccggaaaac tctttatctt cgttacgata aaaaaccagg tcgggtgaag
    60061 catcaaggaa ggaacgtaag aatgaggatt gctgctcgag ctgaatttgt gtctcttcgc
    60121 gctctttgat ttcaattttc agttggccga aggtttcctg cagttctgct tccgcttttt
    60181 cacgaacggc aatttcctga tttagctggg caatattatc ttttaactga acgttgaggc
    60241 tcaaatcgcg ctcgcgcatc tcctccagtt tttgcaccag ccgtgacaga cgttgtcgtg
    60301 actcctccag ttgctcgacg accaccgata gaaagtagac cgcccacggc gtaatcagca
    60361 aaccaaagaa gatagaacga ataacatcaa tgctttcgac ctgaccatgc agcaccatgg
    60421 ttaccgccat ttgtaccaca atggcaagaa cgacgagggc cagcgccagc aacattgaga
    60481 agcgcaccag acctaacttc atcatcaggt caacataata ctgcgccagc agacgaattt
    60541 gcttcattag ggaattcctt cacgacaacc tgccccaata atacccaatt ctgacagtta
    60601 cgttataaat tgtgtgagaa atgcggaatt cccctcaacg acctactccg ttggaggtag
    60661 ccacggacgt cccagcgccg atccctgtac gccatgttca ttcagatagc gatccagctc
    60721 gaccattccc gtccatcgat tttcgcacca cagcggagca agcagcgtcg ggcgacgggc
    60781 gctggcggaa atgcggtggt agatcacttc cggtggcgta tggcgaatca tttctccggc
    60841 agtgagcgta taatcctcca gttcaatacc gttcaaacgc cccgcttccc aggctttcgc
    60901 cataatgctg cctttcacaa tatgcagcgg atgcagcttt atgccgtcca cgctggtttc
    60961 aaccactcgc tccagcgttt gcaaacattc agcctgccct tcgccaggca ggccgacaat
    61021 taagtgggaa cacaccttca gcccgcgctg acgtgccagc tgggttgtac gctgataaca
    61081 ggcaaaatca tggccgcggt tgatgcgatg cagtgttttg tcgtgggcgg tttgtagccc
    61141 cagctccagc cacacttcgt aaccctggtc cttatattcg caaagcagat ccagcaccgc
    61201 atccggcacg cagtccgggc gggtaccaac acacaaaccg acaatattgg cctggctcac
    61261 cgcctgctga tacatagaac gcagcacctg aacttccgca aaggtgctgg tatacgcctg
    61321 aaagtaggcc agatagcgtt tagcgcggtt aactaaattc gcctggtgcg ccagttgctc
    61381 ggcaatggaa cgatgctgct gcgcttcatc ggcaaacgag gcaacattac agaatgtgca
    61441 gccaccacgc ccgatggtac cgtcacggtt agggcagcta aaaccgccgt gcagcgtcag
    61501 cttatgcacc ttttgcccat aacgacgggt gagatcacca ccaaacatat tgactaattt
    61561 ctgtaactgc ataatctgat agaccgcgcc ttgaaaagag gccaaagcct gccattttta
    61621 gctcaattcg gcgatgacct agatcaatcg tcccgccctg cttttatcaa ctgcataatc
    61681 aatcaaaatt accgaaattt catgcataat cacataaatc acttttgctt atcttgtgtc
    61741 agattttttt atctccttat ggattttagg caaaaacagt agcatgaaac gtcattacca
    61801 attaaggcag tataaaatgc tggttttctc gtcagttcaa ggcaggataa gggttaacac
    61861 gcctttatga cagtcaggaa ttgactgttt ctctaacgac tctcctttta gccttaaaga
    61921 taaaatctat tttaatttca gccatttaat aaagaatttt gcgctaaagc acatttctgt
    61981 accaataagc ttgccatttg acctgtatca gctttcccga taagttggaa atccgctgga
    62041 agctttctgg atgagcagcc tgctcatcat atttatgcag taattgagat cccctcttca
    62101 ccgtattaac cgatgcgaaa aggacaacaa gggggcgaat gcgaggcgcg cgtatgacac
    62161 gcaaaccccg tcgccacgct ctttctgtgc ccgtgcgcag cggttcggaa gtggggttcc
    62221 cgcagagcct gggggaggtt cacgatatgt tgtacgataa atcccttgag agggataact
    62281 gtggtttcgg cctgatcgcc cacatagaag gcgaacctag ccacaaggta gtgcgtactg
    62341 caatacacgc actggcccgc atgcagcacc gtggcgcgat tctcgccgat ggtaaaaccg
    62401 gcgacggttg cggcttgctg ttacaaaaac cggatcgctt ttttcgcatc gttgcgcagg
    62461 agcgcggctg gcgtttagca aaaaactacg ctgtcgggat gctcttcctg aataaagatc
    62521 ctgaactcgc cgctgccgca cgccgcatcg ttgaagaaga actgcaacgc gaaaccttgt
    62581 cgattgtggg ctggcgtgat gtccccacta acgaaggcgt gctgggtgaa atcgccctct
    62641 cctctctgcc acgcattgag caaatttttg tgaacgcccc ggcaggctgg cgtccacgcg
    62701 atatggagcg ccgtctgttt atcgcccgcc gccgcattga aaagcgtctc gaagccgaca
    62761 aagacttcta cgtctgtagc ctgtcgaatc tggtgaacat ctataaaggt ctgtgtatgc
    62821 cggcggatct gccgcgcttt tatctggatc ttgcggacct gcgtctggaa tcggccattt
    62881 gcctgttcca ccagcgcttc tccactaaca ccgtaccgcg ctggccgctg gcgcaaccgt
    62941 tccgctatct ggcgcataac ggtgaaatca acaccatcac cggtaaccgc caatgggcgc
    63001 gtgcgcgtac ctataaattc cagacgccgc tgatccctga cctgcacgac gccgcgccat
    63061 tcgtcaacga aaccggttct gactccagtt ccatggataa catgctggaa ctgctgctgg
    63121 caggcgggat ggatatcatc cgcgccatgc gcctgttagt gccacccgcc tggcagaaca
    63181 acccggatat ggacccggaa ctgcgtgcct tctttgactt taactccatg catatggagc
    63241 cgtgggatgg tccggcgggc atcgtgatgt ccgatggtcg cttcgctgcc tgtaacctcg
    63301 accgtaacgg tctgcgtccg gcacgctacg tcattaccaa agacaagctc atcacctgcg
    63361 cctctgaagt cggtatctgg gattaccagc ctgacgaagt ggtcgaaaaa ggccgcgtcg
    63421 ggccaggcga actgatggtg atcgacactc gcagtgggcg tattctgcac tcggcagaaa
    63481 ccgatgacga tctgaaaagc cgccaccctt ataaagagtg gatggagaaa aacgtccgcc
    63541 gactggtacc gtttgaagat ctgcccgatg aagaagtggg tagccgcgaa ctggacgacg
    63601 acacgcttgc cagctaccag aaacagttta actacagcgc ggaagagctg gactccgtaa
    63661 ttcgcgtact gggcgaaaac ggtcaggaag cggtcggttc gatgggcgat gataccccat
    63721 tcgccgtgct ctccagtcag ccgcgcatta tttacgacta cttccgccag cagtttgccc
    63781 aggtgactaa cccgccaatc gacccgctgc gtgaagcgca tgttatgtcg ctcgccacca
    63841 gtatcggtcg tgaaatgaac gtcttttgcg aagcagaggg ccaggcgcac cgtttaagct
    63901 ttaaatcgcc gattctgctc tactccgatt tcaaacagct cacgacgatg aaagaggagc
    63961 actaccgcgc agatacgctg gatatcacct ttgacgtcac taaaaccacg ctcgaagcga
    64021 cagtcaaaga gctatgcgac aaagccgaaa aaatggtacg tagcggcacc gtgttgctgg
    64081 tgctctccga ccggaatatc gctaaagatc gcctgccggt tccggctccg atggcggttg
    64141 gcgcgatcca gacccgtctg gtcgatcaaa gcctgcgttg cgatgccaac atcatcgtcg
    64201 aaaccgccag cgcccgcgat ccgcaccact tcgccgtgtt gctgggtttc ggcgcgacgg
    64261 ctatttatcc gtaccttgcc tatgaaacgc tgggccgcct ggtagacacc cacgcgattg
    64321 ccaaagatta tcgtaccgtg atgctcaact accgtaacgg catcaacaaa ggcttgtaca
    64381 aaatcatgtc caaaatgggc atctccacca tcgcctctta ccgctgctcg aaactgtttg
    64441 aagcggtcgg tctacacgat gatgtagtgg gcctgtgctt ccagggcgcg gtcagccgca
    64501 ttggtggggc aagctttgaa gacttccagc aggatctgct gaatctgtcg aaacgtgcct
    64561 ggctggcgcg caagccgatt agccagggcg gcctgctgaa atacgtccac ggcggcgaat
    64621 accacgccta caacccggac gtggtgcgca cgctgcaaca ggcggtacaa agcggcgagt
    64681 acagcgacta tcaggaatac gcgaagctgg ttaatgagcg tccgacaacc acgctgcgcg
    64741 atctgctggc aattacgccg ggtgaaaacg cggtcaacat tgctgatgtt gaaccggcaa
    64801 gcgaactgtt taaacgcttt gataccgccg cgatgtctat cggcgcgtta agcccggaag
    64861 cccacgaggc gctggcggaa gcgatgaata gcatcggcgg taactcgaac tccggtgaag
    64921 gcggcgaaga cccggcgcgc tatggcacta acaaagtgtc gcgcatcaag caggtagctt
    64981 ctggtcgctt cggcgttact ccggcgtatc tggtcaatgc cgacgtcatt cagattaaag
    65041 tcgcccaggg cgcgaagccg ggcgaaggcg gtcagttgcc gggcgacaaa gtcactcctt
    65101 acatcgccaa actgcgctat tcggtgcccg gagtgacgct gatctccccg ccgccgcacc
    65161 acgatatcta ctctatcgaa gacttagcgc agcttatttt cgacctcaag caggttaacc
    65221 cgaaagcgat gatctccgtt aagctggtgt ccgaaccagg cgttggcacc atcgcgactg
    65281 gcgtggcgaa agcttatgcg gacttgatca ccatcgcggg ctatgacggc ggcaccggcg
    65341 caagcccgct gtcgtcagtg aaatatgcag gctgcccgtg ggaactcggt ctggtggaaa
    65401 cccaacaggc gctggttgcc aacggcttgc gtcataaaat tcgtttgcag gttgatggcg
    65461 gcctgaaaac gggtgtcgat atcatcaaag cggcgattct cggcgcagaa agcttcggct
    65521 tcggcactgg cccaatggtg gcgctcggct gtaaatatct gcgtatttgc cacctgaaca
    65581 actgcgccac gggtgtagca actcaggatg acaaactgcg taagaatcac tatcacggcc
    65641 tgccgttcaa ggtgactaat tactttgagt ttatcgcccg tgaaacccgc gagctgatgg
    65701 cacagctggg agtaacgcgt ctggtggatc tgattggtcg caccgacctg ctgaaagagc
    65761 tggacggttt caccgccaaa cagcagaaac tggcgctgtc gaagctgctg gagactgccg
    65821 agccacatgc aggtaagtca ctctactgca ctgaaaacaa cccgccgttt gataacggcc
    65881 tactgaacgc gcagttgctg caacaggcga aaccgtttgt cgatgaacgc cagagcaaaa
    65941 ccttctggtt cgatatccgc aacaccgacc gttctgtcgg cgcgtcgctt tcaggctata
    66001 tcgcccagac gcacggcgat caggggctgg cagccgatcc tatcaaagcg tacttcaacg
    66061 gcaccgcagg ccagagtttc ggtgtgtgga acgcgggcgg cgtggaacta tacctgaccg
    66121 gggatgccaa cgactatgtc ggtaaaggca tggcgggcgg cttaatcgcc attcatcctc
    66181 cggttggttc cgccttccgc agccatgaag caagcattat cggcaacacc tgcctgtatg
    66241 gcgcgaccgg tggtcgtctg tatgccgcag gccgcgcggg tgaacgtttc ggcgtgcgga
    66301 actccggtgc tatcaccgtg gtagaaggca ttggcgacaa cggctgtgaa tatatgacgg
    66361 gcggtattgt ctgtattctg ggtaaaaccg gcgttaactt cggcgcgggc atgaccggcg
    66421 gcttcgctta cgttctcgat gaaagcggtg atttccgcaa acgcgttaac ccggaactgg
    66481 tcgaagtctt aagcgtcgac gatctggcga tccatgaaga gcatctgcgc ggtcttatca
    66541 ccgagcatgt tcagcatacc ggttctcagc gcggtgaaga gattctggcg aactggtcaa
    66601 ccttcgccac gaaattcgcg ctggttaaac cgaagtccag tgatgtaaaa gcactgctgg
    66661 gtcaccgtag tcgtagcgca gcagagttgc gcgtgcaggc gcagtaaggg gtagcaacaa
    66721 tgagtcagaa tgtttatcaa tttatcgacc tgcagcgcgt tgatccgcca aagaaaccgc
    66781 tgaagatccg taaaattgag tttgttgaaa tttacgagcc gttttccgaa ggccaggcca
    66841 aagcgcaggc tgaccgctgt ctgtcgtgcg gcaacccata ctgcgagtgg aaatgcccgg
    66901 tacacaacta catcccaaac tggctgaagc tcgccaacga ggggcgtatt tttgaagcgg
    66961 cagaactgtc gcatcagacc aacacactgc cggaagtatg cggacgtgtc tgcccgcaag
    67021 accgtctgtg cgaaggttcc tgcaccctga acgatgagtt cggtgcggtg accatcggca
    67081 acattgagcg ttatatcaat gataaagcgt tcgaaatggg ctggcgtccg gatatgtccg
    67141 gcgtgaagca aaccggtaaa aaagtggcga ttatcggtgc aggcccggca ggtctggcgt
    67201 gtgcggatgt cctgacgcgt aacggcgtaa aagccgttgt cttcgaccgt catccagaaa
    67261 ttggcgggct gctgaccttc ggtattccgg ccttcaagct ggaaaaagag gtaatgacgc
    67321 gtcgccgtga aatcttcacc ggcatgggta ttgaattcaa actcaatacc gaagtgggcc
    67381 gcgacgtaca gctggacgat ctgctgagtg attacgatgc cgtgttcctt ggcgttggga
    67441 cttatcagtc aatgcgcggc gggctggaaa acgaagacgc cgatggcgtg tacgcagcgc
    67501 tgccgttcct tatcgccaac accaaacagt taatgggctt tggtgaaacc cgcgacgaac
    67561 cgttcgtcag catggaaggc aaacgcgtgg tagtacttgg cggtggcgac actgcgatgg
    67621 actgcgtgcg cacgtctgta cgccaggggg caaagcacgt tacctgtgcc tatcgtcgtg
    67681 atgaagagaa catgccgggt tcccgccgcg aagtgaaaaa cgcgcgggaa gaaggcgtag
    67741 agttcaaatt caacgtccag ccactgggga ttgaagtgaa cggtaacggc aaagtcagcg
    67801 gcgtaaaaat ggtacgtacc gaaatgggcg aaccggatgc caaaggccgt cgccgcgcgg
    67861 agatcgttgc aggttccgaa catatcgttc cggcagatgc ggtgatcatg gcgtttggtt
    67921 tccgtccaca caacatggaa tggctggcaa aacacagcgt cgagctggat tcacaaggcc
    67981 gcatcatcgc tccggaaggc aacgacaacg ctttccagac cagcaacccg aaaatctttg
    68041 ctggcggcga tatcgtccgt ggttccgatc tggtcgttac cgccatcgcc gaaggtcgca
    68101 aagcggcaga cggcattatg aactggctgg aagtttaagc tctgtcccag cgccgcctcc
    68161 tcagaaaaga gtcggcgctg aaattctcct ttctttctgg aaatcgcctc gcataatctg
    68221 aagaaaacca atgtaatacg ctcatctgga ttgttaccgg tagatgcccg gcgtatagct
    68281 gattccatta ttcaacgcag cgccatataa agggacagat aatggaatcg ctctcagaga
    68341 gaacctcaac aggctaccag caaatccacg acggcattat tcatctggtc gatagcgccc
    68401 gaacggaaac ggtacgtagc gttaacgcgc tgatgaccgc gacgtactgg gaaattggcc
    68461 gacggattgt cgaatttgaa caaggtggtg aggccagagc agcttatggt gcacagttga
    68521 ttgatcggct atcgaaggat ttaagtcgac ggtataaacg tggggtctct gcaaaaaact
    68581 tacgccaaat gaggcttttt tacctctttt ttcaacatgt tgaaattcgc cagacagtgt
    68641 ctggcgaatt aacaccattg ggaattccgc agacaccatc tgcagaattc ccatcggcaa
    68701 agatttggca gacgctgtct gccaaattga gaggctgttt gatatttcca cagcttttgt
    68761 attgcatctc ctattttcgg tttgtatatt catgcaaata aacgaagg
//
LOCUS       DAJTRQ010000006        67993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000006 DAJTRQ010000000
VERSION     DAJTRQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..245
                     /locus_tag="OO332_000489"
     CDS             <1..245
                     /locus_tag="OO332_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5941089.1"
                     /translation="TIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIE
                     QRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO332_000490"
     CDS             complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO332_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5941090.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO332_000491"
     CDS             complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO332_000491"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5941091.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1989..3215
                     /gene="mdfA"
                     /locus_tag="OO332_000492"
     CDS             1989..3215
                     /gene="mdfA"
                     /locus_tag="OO332_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5941092.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3256..3541)
                     /locus_tag="OO332_000493"
                     /pseudo
     CDS             complement(3256..3541)
                     /locus_tag="OO332_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO332_000494"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO332_000494"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5941093.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OO332_000495"
     CDS             complement(4442..5650)
                     /locus_tag="OO332_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941094.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OO332_000496"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OO332_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5941095.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OO332_000497"
     CDS             complement(6445..8130)
                     /locus_tag="OO332_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5941096.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OO332_000498"
     CDS             8400..8777
                     /locus_tag="OO332_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5941097.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO332_000499"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO332_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5941098.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OO332_000500"
     CDS             9224..9511
                     /locus_tag="OO332_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5941099.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9495..10218
                     /gene="nfsA"
                     /locus_tag="OO332_000501"
                     /pseudo
     CDS             9495..10218
                     /gene="nfsA"
                     /locus_tag="OO332_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OO332_000502"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OO332_000502"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5941100.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OO332_000503"
     CDS             11269..11745
                     /locus_tag="OO332_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5941101.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OO332_000504"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OO332_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5941102.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="OO332_000505"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="OO332_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5941103.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OO332_000506"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OO332_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5941104.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OO332_000507"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OO332_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5941105.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OO332_000508"
     CDS             16302..16790
                     /locus_tag="OO332_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5941106.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OO332_000509"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OO332_000509"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5941107.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO332_000510"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO332_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5941108.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO332_000511"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO332_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5941109.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO332_000512"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO332_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5941110.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO332_000513"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO332_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5941111.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO332_000514"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO332_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5941112.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OO332_000515"
     CDS             complement(22242..22757)
                     /locus_tag="OO332_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5941113.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OO332_000516"
     CDS             23406..25175
                     /locus_tag="OO332_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5941114.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OO332_000517"
     CDS             25386..25709
                     /locus_tag="OO332_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5941115.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OO332_000518"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OO332_000518"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5941116.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OO332_000519"
     CDS             complement(26533..27546)
                     /locus_tag="OO332_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5941117.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OO332_000520"
     CDS             complement(27645..29075)
                     /locus_tag="OO332_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5941118.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO332_000521"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO332_000521"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5941119.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO332_000522"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO332_000522"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5941120.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO332_000523"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO332_000523"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5941121.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO332_000524"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO332_000524"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5941122.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO332_000525"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO332_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5941123.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO332_000526"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO332_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5941124.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="OO332_000527"
     CDS             37227..38885
                     /locus_tag="OO332_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5941125.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="OO332_000528"
     CDS             complement(38882..39838)
                     /locus_tag="OO332_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5941126.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="OO332_000529"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="OO332_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5941127.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="OO332_000530"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="OO332_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5941128.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OO332_000531"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OO332_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5941129.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="OO332_000532"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="OO332_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5941130.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="OO332_000533"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="OO332_000533"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5941131.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="OO332_000534"
     tRNA            complement(46697..46784)
                     /locus_tag="OO332_000534"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OO332_000535"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OO332_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5941132.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OO332_000536"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OO332_000536"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5941133.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OO332_000537"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OO332_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5941134.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OO332_000538"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OO332_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5941135.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OO332_000539"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OO332_000539"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5941136.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="OO332_000540"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="OO332_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5941137.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="OO332_000541"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="OO332_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5941138.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="OO332_000542"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="OO332_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5941139.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /gene="rarA"
                     /locus_tag="OO332_000543"
                     /gene_synonym="mgsA"
     CDS             58920..60263
                     /gene="rarA"
                     /locus_tag="OO332_000543"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5941140.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="OO332_000544"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="OO332_000544"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5941141.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="OO332_000545"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="OO332_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="OO332_000546"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="OO332_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5941142.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /gene="dmsC"
                     /locus_tag="OO332_000547"
     CDS             64960..65823
                     /gene="dmsC"
                     /locus_tag="OO332_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR5941143.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="OO332_000548"
     CDS             complement(65859..66485)
                     /locus_tag="OO332_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5941144.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..67948
                     /locus_tag="OO332_000549"
     CDS             66800..67948
                     /locus_tag="OO332_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941145.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16900 a        16820 c        17821 g        16452 t
ORIGIN      
        1 tgaccatacc gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc
       61 agcttaccgc accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg
      121 gtaaatccat tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct
      181 ttggtaggat gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct
      241 cttaatcttc tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat
      301 caacttaata cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact
      361 caccacaata tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt
      421 actgtggtcg acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg
      481 caactcttca agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata
      541 aaaagcgata tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat
      601 ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt
      661 cagcgccaga aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat
      721 cgcttcaata agccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc
      781 taccagtgtc gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg
      841 atcgcttaac aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa
      901 aacgacgggc gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag
      961 cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg
     1021 cccgatacgc tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc
     1081 gctcaatact acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa
     1141 tgcaaaacag acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg
     1201 ccagataatc tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc
     1261 cagcggccag tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat
     1321 taaaagtgag ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat
     1381 caccgtaccg tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc
     1441 gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa
     1501 caggctgacc gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc
     1561 ccacaaccaa agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa
     1621 aatcgccaac gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag
     1681 agagagattc aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat
     1741 acaggcaagt cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt
     1801 tccctcaagc ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta
     1861 aagaaagcgt agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac
     1921 aaaattcgct ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa
     1981 gaaattgcat gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt
     2041 tccctctctg tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac
     2101 ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga
     2161 tgaccgcgta tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc
     2221 gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg
     2281 caatattact ggcgcaaaac attgaacaat tcaccatgtt gcgcttcttg cagggcataa
     2341 gcctctgttt cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg
     2401 tttgtatcaa gatcaccgcg ctgatggtga acgtggcgct gattgctccg ctacttggtc
     2461 cgctggtggg cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg
     2521 ccgcattggc agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccacgc
     2581 gtataggcga gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga
     2641 acggccgctt tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
     2701 cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc
     2761 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
     2821 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
     2881 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
     2941 taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt
     3001 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
     3061 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
     3121 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
     3181 aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga
     3241 tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc
     3301 acctacgctg gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcacagg
     3361 agccacatag acggtttgcg gcacagtcgc gacgctgaca cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattggagc ctgcccggta ttttgccgct
     3721 gcgacgaccg cgctgccggc atttttcatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcactacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatactc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa cggtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgccctgg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaagcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggtgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atggtaattt acgataatgt gttttaccag aattcagggt gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt tttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggtaaaa cgtcttgccc cacagctgat gaaccgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcggct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcac agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgctgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag ttagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaatc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacgtg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggtgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc agaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgtttgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gcaggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgtgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actgacaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatagg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gctgcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aattgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccggcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 tcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccatt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tactgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaat
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt
    67741 tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag
    67801 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
    67861 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca
    67921 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt
    67981 cacactctgc ttt
//
LOCUS       DAJTRQ010000007        66600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000007 DAJTRQ010000000
VERSION     DAJTRQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO332_000550"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO332_000550"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5941146.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO332_000551"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO332_000551"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5941147.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO332_000552"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO332_000552"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5941148.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO332_000553"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO332_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5941149.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO332_000554"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO332_000554"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5941150.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO332_000555"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO332_000555"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5941151.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO332_000556"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO332_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5941152.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO332_000557"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO332_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5941153.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO332_000558"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO332_000558"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5941154.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO332_000559"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO332_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5941155.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO332_000560"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO332_000560"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5941156.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO332_000561"
     CDS             complement(15665..16702)
                     /locus_tag="OO332_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5941157.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO332_000562"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO332_000562"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5941158.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO332_000563"
                     /pseudo
     CDS             complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO332_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19424..19864
                     /locus_tag="OO332_000564"
     CDS             19424..19864
                     /locus_tag="OO332_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5941159.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO332_000565"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO332_000565"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5941160.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20591..21581)
                     /locus_tag="OO332_000566"
                     /pseudo
     CDS             complement(20591..21581)
                     /locus_tag="OO332_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO332_000567"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO332_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5941161.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO332_000568"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO332_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5941162.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO332_000569"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO332_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5941163.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO332_000570"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO332_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5941164.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO332_000571"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO332_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5941165.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO332_000572"
     CDS             complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO332_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5941166.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO332_000573"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO332_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5941167.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO332_000574"
     CDS             complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO332_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5941168.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="OO332_000575"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="OO332_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5941169.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO332_000576"
     CDS             complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO332_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5941170.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO332_000577"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO332_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5941171.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO332_000578"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO332_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5941172.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO332_000579"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO332_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5941173.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO332_000580"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO332_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5941174.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="OO332_000581"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="OO332_000581"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5941175.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="OO332_000582"
     CDS             37392..37661
                     /locus_tag="OO332_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5941176.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="OO332_000583"
     CDS             complement(37664..38023)
                     /locus_tag="OO332_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5941177.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="OO332_000584"
     CDS             38164..38790
                     /locus_tag="OO332_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5941178.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="OO332_000585"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="OO332_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5941179.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO332_000586"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO332_000586"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5941180.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="OO332_000587"
     CDS             41630..42295
                     /locus_tag="OO332_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5941181.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="OO332_000588"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="OO332_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5941182.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="OO332_000589"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="OO332_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="OO332_000590"
     CDS             44278..45327
                     /locus_tag="OO332_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5941183.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="OO332_000591"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="OO332_000591"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5941184.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="OO332_000592"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="OO332_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5941185.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="OO332_000593"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="OO332_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5941186.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="OO332_000594"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="OO332_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5941187.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="OO332_000595"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="OO332_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5941188.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="OO332_000596"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="OO332_000596"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5941189.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="OO332_000597"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="OO332_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5941190.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="OO332_000598"
     CDS             complement(51522..51881)
                     /locus_tag="OO332_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5941191.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="OO332_000599"
     CDS             complement(51878..52153)
                     /locus_tag="OO332_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5941192.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="OO332_000600"
     CDS             complement(52212..53336)
                     /locus_tag="OO332_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5941193.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO332_000601"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO332_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5941194.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="OO332_000602"
     CDS             complement(56068..57135)
                     /locus_tag="OO332_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5941195.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="OO332_000603"
     CDS             complement(57501..59123)
                     /locus_tag="OO332_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5941196.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="OO332_000604"
     CDS             complement(59385..60974)
                     /locus_tag="OO332_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5941197.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61358..62389
                     /locus_tag="OO332_000605"
                     /pseudo
     CDS             61358..62389
                     /locus_tag="OO332_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62923..63687)
                     /locus_tag="OO332_000606"
     CDS             complement(62923..63687)
                     /locus_tag="OO332_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5941198.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="OO332_000607"
     CDS             63934..64695
                     /locus_tag="OO332_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5941199.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64802..65302)
                     /locus_tag="OO332_000608"
     CDS             complement(64802..65302)
                     /locus_tag="OO332_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941200.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65453..>66600)
                     /locus_tag="OO332_000609"
     CDS             complement(65453..>66600)
                     /locus_tag="OO332_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5941201.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16176 a        17873 c        16342 g        16209 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacga
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc taggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggctg ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgatattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcgc
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcgcg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcgcggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaatctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acgcacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggtgt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaactg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcacttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacagg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcagttttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagcgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg cgtttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt tttattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgaacgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagctat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtgatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg gaaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaagattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattaaat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg actttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag ggggatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagt
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tgcttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    61201 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    61261 acatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    61321 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    61381 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    61441 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    61501 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
    61561 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    61621 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    61681 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    61741 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    61801 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    61861 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    61921 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    61981 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    62041 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    62101 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    62161 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    62221 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    62281 gctggactac tttattccat ctcgggttct ggttggatta agcgcagtat gttttacatt
    62341 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    62401 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    62461 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    62521 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    62581 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    62641 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    62701 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    62761 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    62821 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    62881 gagagcaaaa aaacacacct gtagcaaaaa tacattttct tattagtcat ataatagcac
    62941 ctctgaacaa attgcataag tttcgccatt agaggatttt atacatcttg ataaataccg
    63001 ctgcccttcc ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg
    63061 cacacagggt aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgaggcc
    63121 aatctcgtta agattcgctg aaagatataa atcacaggtg tcaatcttac ctgatttaac
    63181 caatggataa tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat
    63241 aacaattact accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc
    63301 tactttttca acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt
    63361 tatgttttca ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac
    63421 accaatattt ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga
    63481 cagatcaaat gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc
    63541 accattactt tccagtaaca ctgccaaaat acaccccgcc gtagctggca aatttatctc
    63601 atcctgggta tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt
    63661 gataatgaaa aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt
    63721 ttagcctatt cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa
    63781 gtcatataaa acttatatcc acagaataaa aaaacaaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcttcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttctgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
    66541 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagaca
//
LOCUS       DAJTRQ010000008        65603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000008 DAJTRQ010000000
VERSION     DAJTRQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(119..598)
                     /gene="folK"
                     /locus_tag="OO332_000610"
     CDS             complement(119..598)
                     /gene="folK"
                     /locus_tag="OO332_000610"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5941202.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(595..2013)
                     /gene="pcnB"
                     /locus_tag="OO332_000611"
     CDS             complement(595..2013)
                     /gene="pcnB"
                     /locus_tag="OO332_000611"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5941203.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2052..2978)
                     /gene="gluQRS"
                     /locus_tag="OO332_000612"
     CDS             complement(2052..2978)
                     /gene="gluQRS"
                     /locus_tag="OO332_000612"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5941204.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3015..3470)
                     /gene="dksA"
                     /locus_tag="OO332_000613"
     CDS             complement(3015..3470)
                     /gene="dksA"
                     /locus_tag="OO332_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5941205.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3648..4352)
                     /gene="sfsA"
                     /locus_tag="OO332_000614"
     CDS             complement(3648..4352)
                     /gene="sfsA"
                     /locus_tag="OO332_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5941206.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4367..4897)
                     /gene="thpR"
                     /locus_tag="OO332_000615"
     CDS             complement(4367..4897)
                     /gene="thpR"
                     /locus_tag="OO332_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5941207.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4971..7400
                     /gene="hrpB"
                     /locus_tag="OO332_000616"
     CDS             4971..7400
                     /gene="hrpB"
                     /locus_tag="OO332_000616"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5941208.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7602..10136
                     /gene="mrcB"
                     /locus_tag="OO332_000617"
     CDS             7602..10136
                     /gene="mrcB"
                     /locus_tag="OO332_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5941209.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10356..12599
                     /gene="fhuA"
                     /locus_tag="OO332_000618"
     CDS             10356..12599
                     /gene="fhuA"
                     /locus_tag="OO332_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5941210.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12650..13447
                     /gene="fhuC"
                     /locus_tag="OO332_000619"
     CDS             12650..13447
                     /gene="fhuC"
                     /locus_tag="OO332_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5941211.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13447..14337
                     /gene="fhuD"
                     /locus_tag="OO332_000620"
     CDS             13447..14337
                     /gene="fhuD"
                     /locus_tag="OO332_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5941212.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14334..16316
                     /gene="fhuB"
                     /locus_tag="OO332_000621"
     CDS             14334..16316
                     /gene="fhuB"
                     /locus_tag="OO332_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5941213.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16351..17631)
                     /gene="hemL"
                     /locus_tag="OO332_000622"
     CDS             complement(16351..17631)
                     /gene="hemL"
                     /locus_tag="OO332_000622"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5941214.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17856..19277
                     /gene="clcA"
                     /locus_tag="OO332_000623"
     CDS             17856..19277
                     /gene="clcA"
                     /locus_tag="OO332_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5941215.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19301..19366
                     /locus_tag="OO332_000624"
     CDS             19301..19366
                     /locus_tag="OO332_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941216.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19359..19703
                     /gene="erpA"
                     /locus_tag="OO332_000625"
     CDS             19359..19703
                     /gene="erpA"
                     /locus_tag="OO332_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5941217.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19750..20373)
                     /locus_tag="OO332_000626"
     CDS             complement(19750..20373)
                     /locus_tag="OO332_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5941218.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20411..21211)
                     /gene="btuF"
                     /locus_tag="OO332_000627"
     CDS             complement(20411..21211)
                     /gene="btuF"
                     /locus_tag="OO332_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5941219.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21204..21902)
                     /gene="mtnN"
                     /locus_tag="OO332_000628"
     CDS             complement(21204..21902)
                     /gene="mtnN"
                     /locus_tag="OO332_000628"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5941220.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21986..23503
                     /gene="dgt"
                     /locus_tag="OO332_000629"
     CDS             21986..23503
                     /gene="dgt"
                     /locus_tag="OO332_000629"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5941221.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23633..25057
                     /gene="degP"
                     /locus_tag="OO332_000630"
     CDS             23633..25057
                     /gene="degP"
                     /locus_tag="OO332_000630"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5941222.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25212..26368
                     /gene="cdaR"
                     /locus_tag="OO332_000631"
                     /pseudo
     CDS             25212..26368
                     /gene="cdaR"
                     /locus_tag="OO332_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26457..26843)
                     /locus_tag="OO332_000632"
     CDS             complement(26457..26843)
                     /locus_tag="OO332_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5941223.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27157..27981)
                     /gene="dapD"
                     /locus_tag="OO332_000633"
     CDS             complement(27157..27981)
                     /gene="dapD"
                     /locus_tag="OO332_000633"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5941224.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28012..30684)
                     /gene="glnD"
                     /locus_tag="OO332_000634"
     CDS             complement(28012..30684)
                     /gene="glnD"
                     /locus_tag="OO332_000634"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5941225.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30746..31540)
                     /gene="map"
                     /locus_tag="OO332_000635"
     CDS             complement(30746..31540)
                     /gene="map"
                     /locus_tag="OO332_000635"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5941226.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31908..32633
                     /gene="rpsB"
                     /locus_tag="OO332_000636"
     CDS             31908..32633
                     /gene="rpsB"
                     /locus_tag="OO332_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5941227.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32891..33742
                     /gene="tsf"
                     /locus_tag="OO332_000637"
     CDS             32891..33742
                     /gene="tsf"
                     /locus_tag="OO332_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5941228.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33889..34614
                     /gene="pyrH"
                     /locus_tag="OO332_000638"
     CDS             33889..34614
                     /gene="pyrH"
                     /locus_tag="OO332_000638"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5941229.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34906..35463
                     /gene="frr"
                     /locus_tag="OO332_000639"
     CDS             34906..35463
                     /gene="frr"
                     /locus_tag="OO332_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5941230.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35555..36751
                     /gene="ispC"
                     /locus_tag="OO332_000640"
     CDS             35555..36751
                     /gene="ispC"
                     /locus_tag="OO332_000640"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5941231.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36940..37698
                     /gene="ispU"
                     /locus_tag="OO332_000641"
                     /gene_synonym="uppS"
     CDS             36940..37698
                     /gene="ispU"
                     /locus_tag="OO332_000641"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5941232.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37711..38568
                     /gene="cdsA"
                     /locus_tag="OO332_000642"
     CDS             37711..38568
                     /gene="cdsA"
                     /locus_tag="OO332_000642"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5941233.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38580..39932
                     /gene="rseP"
                     /locus_tag="OO332_000643"
     CDS             38580..39932
                     /gene="rseP"
                     /locus_tag="OO332_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5941234.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39962..42394
                     /gene="bamA"
                     /locus_tag="OO332_000644"
     CDS             39962..42394
                     /gene="bamA"
                     /locus_tag="OO332_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5941235.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42516..43001
                     /gene="skp"
                     /locus_tag="OO332_000645"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42516..43001
                     /gene="skp"
                     /locus_tag="OO332_000645"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5941236.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43005..44030
                     /gene="lpxD"
                     /locus_tag="OO332_000646"
     CDS             43005..44030
                     /gene="lpxD"
                     /locus_tag="OO332_000646"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5941237.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44135..44590
                     /gene="fabZ"
                     /locus_tag="OO332_000647"
     CDS             44135..44590
                     /gene="fabZ"
                     /locus_tag="OO332_000647"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5941238.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44594..45382
                     /gene="lpxA"
                     /locus_tag="OO332_000648"
     CDS             44594..45382
                     /gene="lpxA"
                     /locus_tag="OO332_000648"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5941239.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45382..46530
                     /gene="lpxB"
                     /locus_tag="OO332_000649"
     CDS             45382..46530
                     /gene="lpxB"
                     /locus_tag="OO332_000649"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5941240.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46527..47123
                     /gene="rnhB"
                     /locus_tag="OO332_000650"
     CDS             46527..47123
                     /gene="rnhB"
                     /locus_tag="OO332_000650"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5941241.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47160..50642
                     /gene="dnaE"
                     /locus_tag="OO332_000651"
     CDS             47160..50642
                     /gene="dnaE"
                     /locus_tag="OO332_000651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5941242.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50655..51614
                     /gene="accA"
                     /locus_tag="OO332_000652"
     CDS             50655..51614
                     /gene="accA"
                     /locus_tag="OO332_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5941243.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51713..53854
                     /gene="ldcC"
                     /locus_tag="OO332_000653"
     CDS             51713..53854
                     /gene="ldcC"
                     /locus_tag="OO332_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5941244.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53911..54300
                     /locus_tag="OO332_000654"
     CDS             53911..54300
                     /locus_tag="OO332_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5941245.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54365..55663
                     /gene="tilS"
                     /locus_tag="OO332_000655"
     CDS             54365..55663
                     /gene="tilS"
                     /locus_tag="OO332_000655"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5941246.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55712..55972)
                     /gene="rof"
                     /locus_tag="OO332_000656"
     CDS             complement(55712..55972)
                     /gene="rof"
                     /locus_tag="OO332_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5941247.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55959..56159)
                     /locus_tag="OO332_000657"
     CDS             complement(55959..56159)
                     /locus_tag="OO332_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5941248.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56325..56858
                     /locus_tag="OO332_000658"
     CDS             56325..56858
                     /locus_tag="OO332_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5941249.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56867..57289
                     /gene="arfB"
                     /locus_tag="OO332_000659"
     CDS             56867..57289
                     /gene="arfB"
                     /locus_tag="OO332_000659"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5941250.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57303..58013
                     /gene="nlpE"
                     /locus_tag="OO332_000660"
                     /gene_synonym="cutF"
     CDS             57303..58013
                     /gene="nlpE"
                     /locus_tag="OO332_000660"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5941251.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58168..58992)
                     /locus_tag="OO332_000661"
     CDS             complement(58168..58992)
                     /locus_tag="OO332_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5941252.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59045..60763)
                     /gene="proS"
                     /locus_tag="OO332_000662"
     CDS             complement(59045..60763)
                     /gene="proS"
                     /locus_tag="OO332_000662"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5941253.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60874..61581)
                     /gene="tsaA"
                     /locus_tag="OO332_000663"
     CDS             complement(60874..61581)
                     /gene="tsaA"
                     /locus_tag="OO332_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5941254.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61578..61982)
                     /gene="rcsF"
                     /locus_tag="OO332_000664"
     CDS             complement(61578..61982)
                     /gene="rcsF"
                     /locus_tag="OO332_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5941255.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62100..62915)
                     /gene="metQ"
                     /locus_tag="OO332_000665"
     CDS             complement(62100..62915)
                     /gene="metQ"
                     /locus_tag="OO332_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5941256.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62955..63608)
                     /gene="metI"
                     /locus_tag="OO332_000666"
     CDS             complement(62955..63608)
                     /gene="metI"
                     /locus_tag="OO332_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5941257.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63601..64632)
                     /gene="metN"
                     /locus_tag="OO332_000667"
     CDS             complement(63601..64632)
                     /gene="metN"
                     /locus_tag="OO332_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5941258.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64820..65395
                     /gene="gmhB"
                     /locus_tag="OO332_000668"
     CDS             64820..65395
                     /gene="gmhB"
                     /locus_tag="OO332_000668"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5941259.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15508 a        16251 c        18077 g        15767 t
ORIGIN      
        1 tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta aaatttttat
       61 taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata taaataaatt
      121 accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac gtctccccat
      181 caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat ccacgattct
      241 tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta ccaaacagca
      301 tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca cgaccctgtt
      361 gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa gtttcaagcg
      421 ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa
      481 acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc agggcagcat
      541 tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc actgtcatgt
      601 ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac gcggtgacgg
      661 ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa cctggaactc
      721 accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag ctcgcaaggc
      781 caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg cgcgtttacc
      841 ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg tcgtcagacg
      901 tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa
      961 agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg
     1021 gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt tatggatacg
     1081 cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt cgccattttc
     1141 cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt cacacaacag
     1201 cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt caaacaggcg
     1261 tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg cggtttccgg
     1321 gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac gtaccggatc
     1381 ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct tcagatcctt
     1441 cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca ggctgttgat
     1501 agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa
     1561 catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt cgtggtgtcc
     1621 acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca gacggaaacg
     1681 gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg tggcgttagt
     1741 ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac
     1801 cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg cattttcact
     1861 gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct gtggacgggc
     1921 gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac
     1981 tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg
     2041 ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc cgactcgggc
     2101 acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac gctgaaatcc
     2161 tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag taccgggcgt
     2221 ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc gccttgtgga
     2281 ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag ctggtacagc
     2341 gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc tgtaacgccc
     2401 tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc acggcgatga
     2461 atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg cagcaggtca
     2521 gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc tggtccatga
     2581 tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgta
     2641 caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc acgataggcg
     2701 tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta atgttccagc
     2761 tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat atcttctatg
     2821 cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag cgcggcgatc
     2881 agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat atactgtgtg
     2941 tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg gtaaacatga
     3001 tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag cgttttgcag
     3061 tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat ttcaacaccg
     3121 caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt ctcgatcttt
     3181 ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc ttcttcctgg
     3241 gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg tgtaacggtg
     3301 cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg gaagtgcgcc
     3361 agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc caccccagcg
     3421 atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat gttgcttctc
     3481 cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta taaatagcag
     3541 atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg aggaaaagcg
     3601 tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta caatgtaacc
     3661 ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta agccagaatt
     3721 tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc gatgtggcgc
     3781 gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac cgcacgctgg
     3841 ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc agtgaccgca
     3901 tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc aatatagcag
     3961 tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga gccgtatttt
     4021 acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt aaggatagcc
     4081 tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc gctctggctt
     4141 tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt cgaataccag
     4201 acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca gtgtagcgtt
     4261 aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc
     4321 gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat tgcgttagcg
     4381 cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg
     4441 tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc accgcctcgc
     4501 tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga aaacaaccgc
     4561 tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt ggaggctgac
     4621 gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca agcgtgagtg
     4681 tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct ttctctgcgc
     4741 tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc ggacgtcccg
     4801 cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt tctgcaggta
     4861 agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg ttatgaatta
     4921 ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt
     4981 tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg ccgcaggtat
     5041 tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa ctgctggcgc
     5101 atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg gcgcgtaacg
     5161 tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt ggctaccgga
     5221 tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc gaaggcgtgc
     5281 tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg atccttgatg
     5341 agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc gatgtgcaac
     5401 aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac aatgaccgct
     5461 tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg tttccggttg
     5521 aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt gcggtagcca
     5581 ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct ggcgtcggag
     5641 aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg gtgctctgcc
     5701 cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag
     5761 ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc atcgaaggta
     5821 ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg cgcacggggc
     5881 ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt gccgggcgcg
     5941 ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa gcagaacgcg
     6001 ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg ctgatggaat
     6061 tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa ccgccaacgg
     6121 tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag ggtgaacggc
     6181 ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta gcggcaatgc
     6241 tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc gccattctcg
     6301 aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag
     6361 cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag
     6421 acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc
     6481 gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc gatgccgacg
     6541 acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc agcgcctcgc
     6601 cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta caacgctgtc
     6661 cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag
     6721 cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg gcgaaaccgt
     6781 cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt ttaagcgtgc
     6841 tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt
     6901 tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg ctggaaacgt
     6961 ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca ctcgacattt
     7021 atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat agtgaattgc
     7081 ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat gaagataacc
     7141 cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat ccgacgatcg
     7201 cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg ccgctgcaaa
     7261 tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa aaggagatga
     7321 aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca ccgacgcgac
     7381 ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga gggccggggt
     7441 gagggcatca gcgcgcacgt tcaccctcac cctaacccta accctctccc tcaagggaga
     7501 ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca
     7561 gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg
     7621 agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc
     7681 gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc
     7741 cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat
     7801 ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc
     7861 tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc gcggcagttt
     7921 atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa atggtgaagc
     7981 tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg
     8041 tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac agtaaagaag
     8101 ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcggccatc gtcaatatgg
     8161 agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc atgatctcct
     8221 cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat ttgctggtgg
     8281 atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc agtctctact
     8341 ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag ggtgcgagta
     8401 cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct tactggcgta
     8461 aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc
     8521 ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct
     8581 tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc ctcgaccagc
     8641 aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg cgtaacccga
     8701 aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa cagattattg
     8761 atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg cgtggtgggg
     8821 tgatctctcc tcagccagcc tttatgcagt tggtgcgtca ggagttgcag gcaaaactgg
     8881 gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc
     8941 aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt
     9001 tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga
     9061 tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt
     9121 cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag ccgaaaatct
     9181 atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg aatggccagg
     9241 tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg
     9301 atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc
     9361 ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg catccggttc
     9421 cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcattccaga
     9481 ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta atcgcggaag
     9541 atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg gcgcaggcgg
     9601 cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt cgtcagcttg
     9661 gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag
     9721 atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta ggccgtgata
     9781 acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat cagcgttatc
     9841 tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg
     9901 gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga
     9961 ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg tcaggcaatc
    10021 cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct cagcaagagc
    10081 agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt aattaacatc
    10141 taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc atctaagata
    10201 ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca gattgcgtac
    10261 cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat cattctcgtt
    10321 tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa actgctcagc
    10381 caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc ggcatgtctg
    10441 tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc gctgcacctg
    10501 cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag tcagctaccg
    10561 gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt gtgaccgccg
    10621 aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac acgccgggtg
    10681 tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt cgcggttttg
    10741 cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag ggcaacttct
    10801 ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg cgtggcccgg
    10861 tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc agcaagcgtc
    10921 cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc ctgttccaga
    10981 ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat cgcctgaccg
    11041 gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt tatgctattg
    11101 caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt tcttacttcc
    11161 agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc gttgagccgc
    11221 tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac aacacctatt
    11281 ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac acctttactg
    11341 tgcgtcagaa cctgcgcttt ggtgaaaaca aaacctcgca aaacagcgtt tatggttacg
    11401 gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta gcgccagcgg
    11461 ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg caaaacttct
    11521 ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac accctgctga
    11581 ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt tacgacgact
    11641 ctgtgccact gctcaatctg tacaatccgg tgaatcccga tttcgacttc aatgccaaag
    11701 atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg ggcgtttatg
    11761 ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt tatgactggg
    11821 cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat gacaaacagt
    11881 ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct tacttcagct
    11941 atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt ttcgcaccgt
    12001 ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt ccgattgtag
    12061 ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac cctgagggtt
    12121 ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc gaagcgaaag
    12181 cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat gcggaataca
    12241 ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac atggcttcgc
    12301 tgtgggctga ctataccttc tttgacggtc cgctttcagg tttgacgctg ggcaccggtg
    12361 gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg ggaagttata
    12421 cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg ggctccaacg
    12481 tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc tttaacactt
    12541 atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt cgtttctaat
    12601 ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta tgcaggaata
    12661 cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg tgcccgggcg
    12721 cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg gtctgattgg
    12781 tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc agcctccgtc
    12841 agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca aagcgtttgc
    12901 ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga ccgtgcgtga
    12961 actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg gtgcggcaga
    13021 tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg cgcatcggct
    13081 ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc tggtggcgca
    13141 ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg cccaccaggt
    13201 tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg tcattgccgt
    13261 gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc tgcgcagtgg
    13321 tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc tcgaaatgat
    13381 ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg tgagttttgt
    13441 ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc gatggcgctt
    13501 tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc caatcgtatt
    13561 gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt gccttacggc
    13621 gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc ggactcagtg
    13681 attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat gaaaccgtcg
    13741 tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg tattgcgccg
    13801 ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg taaatcgctg
    13861 acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt agcgcaatac
    13921 gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg cccgttactg
    13981 ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag cttgttccag
    14041 gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa cttctggggc
    14101 agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt gctctgtttt
    14161 gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg gcaggccatg
    14221 ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta tggtgcaacg
    14281 ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa agcgtgagta
    14341 aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc gctacggcgc
    14401 tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag gccgcctggt
    14461 cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg ccgcgtctgg
    14521 cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt cagcaagtac
    14581 tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg caactgggga
    14641 ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt gctgcgctgg
    14701 caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa cggctgtcgc
    14761 cggtaacgct gattctcgcg gggttggtag tgagccttta ttgcggcgca atcaatcagt
    14821 tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc actggaacgc
    14881 tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg ggcggtgtga
    14941 tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat ggcgtggcgc
    15001 gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg gcgattgtca
    15061 tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg ttcgcgccgc
    15121 tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg gcgtcgttga
    15181 ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact cgcgtgtgga
    15241 tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta ctgtggctgc
    15301 tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt gtcgcgactg
    15361 aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg atggctgtgg
    15421 tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc ggggcgttgc
    15481 tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt gcgggcgtca
    15541 tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg gcaagcccgg
    15601 aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg tttctggtac
    15661 cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg gtgatgctgt
    15721 tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg ttactggcgg
    15781 ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca agtggtgacc
    15841 cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg accgatgctc
    15901 aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg ctgtgccgcc
    15961 gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg atggcgctga
    16021 cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc gcgacgatga
    16081 ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg atgatgggct
    16141 ttcgacggac gatgccacat atcgtaattt cggcgctggt gggtggttta ctgctggtgt
    16201 tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg gggctgctgt
    16261 cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc cgttaataga
    16321 atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc gacgtgcagc
    16381 atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc ccgcttcaaa
    16441 cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac gcttaaaacg
    16501 ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa
    16561 gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt cttctgccgc
    16621 ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt gaatgcccgg
    16681 ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt taccggaaag
    16741 cgtacccgcc tgatagaccg gacctgtagg ggccagcgca tccattacat cacgacgacc
    16801 accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg ttaaatctgg
    16861 cactacgccg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg tcatcacttc
    16921 atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg gcaggaactc
    16981 tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa tacaggcaat
    17041 ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat aagtacaggt
    17101 taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc ctaacgtgag
    17161 tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac aaccttcaaa
    17221 tttaatgatt ttgtcgcgac cggtaaaacc acgggccaag cggatggcgc tcatcgtcgc
    17281 ctcggtgccg gagttcacca tgcgcaccat atctatggtc gggaccagtt cggtcatcag
    17341 ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac gctcggcggc
    17401 ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg gcccccagga
    17461 accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt
    17521 ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc
    17581 gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc
    17641 ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa gttaaatact
    17701 cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt
    17761 gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg
    17821 tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag
    17881 aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg
    17941 ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt atcgggctgg
    18001 ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggagtgctgg
    18061 tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt tcggcggtgc
    18121 tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg ggatcgggga
    18181 tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg cgtgtattgc
    18241 cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta gggcgcgaag
    18301 gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt ttccgcctga
    18361 aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg
    18421 cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc
    18481 gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg accattatgt
    18541 accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc
    18601 ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt
    18661 ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta
    18721 ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg gggtttgtgg
    18781 caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca
    18841 gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta ctctgcttct
    18901 cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa
    18961 ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag gcggggacgt
    19021 ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga
    19081 tcattctggt tctggagatg accgataact accagctcat tttgccaatg attattaccg
    19141 gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac tcggcgattc
    19201 ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag gccgcatcag
    19261 ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt
    19321 agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc
    19381 cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata
    19441 acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg
    19501 gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg
    19561 gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt gactacaccg
    19621 aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt
    19681 gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta tccggcacat
    19741 cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg
    19801 aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag
    19861 tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt
    19921 tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagtacat cacgaatgat
    19981 cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac cggcttccgc
    20041 attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg cgtccagcac
    20101 cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct
    20161 ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc
    20221 cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca gtaccagaac
    20281 accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg caaataccgc
    20341 tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga gcacctgaaa
    20401 aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct gcgaggataa
    20461 tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct
    20521 gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatcg
    20581 cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt
    20641 ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc gactcttttc
    20701 cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag
    20761 catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct tgttcggcct
    20821 tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt tgttcaatgc
    20881 ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct
    20941 cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca
    21001 gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg
    21061 aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg
    21121 gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga aaagacaggg
    21181 cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag tgactcaacc
    21241 atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt
    21301 tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag
    21361 acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg
    21421 attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt
    21481 acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca
    21541 gctttaaagc ctgtcggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca
    21601 tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt cagcgttggt
    21661 gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc
    21721 agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg
    21781 gttccattca gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt
    21841 tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc
    21901 atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc ttacggggaa
    21961 gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg
    22021 gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt
    22081 cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac
    22141 ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga
    22201 agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga aagagcttaa
    22261 attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca ttgttgagat
    22321 gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat
    22381 aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga
    22441 cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc ttaacgagct
    22501 gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct
    22561 ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta ttttaaaata
    22621 tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa
    22681 gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag aacttaattt
    22741 ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta
    22801 ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg agcagcttta
    22861 tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt cgctggtggt
    22921 tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt
    22981 ttttatgtat ttacgggtaa acaccctaaa caaactggta ccctacgcgg cacaacgatt
    23041 tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat tggaagatgc
    23101 cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac atgtatttag
    23161 ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg gattattaga
    23221 gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg tagaaaaaga
    23281 acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga cgcgccatcg
    23341 gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt ttccgctatg
    23401 ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga ccgacctcta
    23461 tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct tttgtaaaga
    23521 cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg ctataaaacg
    23581 aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat acatgaaaaa
    23641 aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat ctccgctctc
    23701 tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa gccttgcacc
    23761 gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta gcacaaccgt
    23821 taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt ctccgttctg
    23881 ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg gcggtaatgg
    23941 cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg atgccgataa
    24001 aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta aagtccagct
    24061 gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc
    24121 gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg attctgatgc
    24181 actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg gcgagacggt
    24241 aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa actacgaaaa
    24301 cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc tggttaacct
    24361 gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg
    24421 tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga tggtggaata
    24481 cgggcaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt ccgaactggc
    24541 gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc tgcctaattc
    24601 ctcggctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga acggtaagcc
    24661 gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg gcagcaaact
    24721 gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac tgcagcagag
    24781 cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg ctgagatgag
    24841 caacaaaggt aaagatcagg gcgtggtagt gaacaacgtg aagacgggca ctccggctgc
    24901 gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg cagtgaaaaa
    24961 catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac tgaacattca
    25021 gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc cttcctgaat
    25081 acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac atcttcatca
    25141 tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa gccgtgcata
    25201 acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat atcgtggcac
    25261 gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg cgaattatcg
    25321 gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg gtactttcac
    25381 agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt gtgcggcagg
    25441 ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg
    25501 aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa atgatgctgg
    25561 aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa gaactggtga
    25621 tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc aacggctggg
    25681 gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg
    25741 cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta cgcccgagcg
    25801 taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac cggcgttgaa
    25861 ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac tgattacccg
    25921 catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt ttaccggtcc
    25981 tagcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg gtaaacagcg
    26041 gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag
    26101 tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaagcgat
    26161 ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca atgtgcaacc
    26221 gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc ggcttaatcg
    26281 tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt
    26341 ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg gcgctggctc
    26401 attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat tcgggattat
    26461 ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt tacgactgac
    26521 tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct gcggtcacgc
    26581 tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat ttcctggact
    26641 tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg cggatattta
    26701 aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat tttcagaata
    26761 tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt ggtaataccc
    26821 agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa ttgtgcttat
    26881 tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct ttttcttcca
    26941 gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg atattccatg
    27001 ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga aacaagatga
    27061 aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg agccagaaag
    27121 catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac gcagcagttc
    27181 gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa ccgcacagta
    27241 gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag accccgccgg
    27301 aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc tctgaccaat
    27361 gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa ccacttcaga
    27421 gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca gcggctccag
    27481 cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct gcgcacaaga
    27541 accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc cgatgttaac
    27601 gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta ccgccgctgg
    27661 tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg cgaatttcat
    27721 cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat cattaatacg
    27781 gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac cgtcaatttt
    27841 ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta ccgcttcgcg
    27901 ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag cggtttcaat
    27961 aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac atcacccttt
    28021 atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca actcgttatt
    28081 aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct cgccaatggt
    28141 tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc cgactcgcgc
    28201 cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt cggtatgggt
    28261 cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg gttggcgacg
    28321 gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa accgaataac
    28381 ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa aggtatccat
    28441 cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat tgcggcggtc
    28501 taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga taaaaatctc
    28561 ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta aatcatgctg
    28621 taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa aatagttagc
    28681 acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca tgcgcagtag
    28741 tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg tgttttgcat
    28801 tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac tttgcttcca
    28861 gctattccac agcgtttcgt tggtggcgca aatgtcagcc actgtcaggc ataccagata
    28921 gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt cagggtcctg
    28981 aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca gccaggcaac
    29041 cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta catcctgagc
    29101 accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca gcgcggcgat
    29161 gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg gatggcgctg
    29221 gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag tatgttcatc
    29281 caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg accattgcgg
    29341 catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc gcaccgctcc
    29401 ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac acagcggttg
    29461 ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa taccggtgat
    29521 cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct ggcgcataaa
    29581 tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat cgtcgagtgg
    29641 acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt gcagcagcat
    29701 ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca tccgctcgac
    29761 cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt ggcgatcgaa
    29821 taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa tacgccacag
    29881 tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc caaacccgac
    29941 catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca gagtgtgaat
    30001 atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc tggtgccatg
    30061 gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact tgtcggaagg
    30121 ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa catcgccaat
    30181 taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc cttccaacat
    30241 gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc agagcagcgt
    30301 taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc ttaaaatcag
    30361 taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga cggcaaccaa
    30421 tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca ggagctggtc
    30481 tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt caaaggcatc
    30541 acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg ttaattcatc
    30601 acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag cggtgtttgc
    30661 gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta tcgcattaaa
    30721 aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc gggatggtgt
    30781 catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata gtatgctcat
    30841 attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc atggtgcgga
    30901 tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc ggtttcagca
    30961 cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg aagccgcgac
    31021 caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg acaaatttct
    31081 gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt agcgccaggt
    31141 acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc ttaccgacga
    31201 taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca tcaatgttaa
    31261 cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac accacttcat
    31321 taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca gaaaccgcgt
    31381 gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg ctgacgcccg
    31441 gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca gcgacgcgca
    31501 ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc catcagcgtc
    31561 ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg gcaaattgag
    31621 aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga aaattcgacg
    31681 tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg tggtataaag
    31741 cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac aatctcactt
    31801 tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg tcggtaatat
    31861 gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg gcaactgttt
    31921 ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt tactggaacc
    31981 cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc aaccttgaga
    32041 aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt tctcgcaaag
    32101 gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa gacgctgctc
    32161 tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg actaactgga
    32221 aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct caggacggta
    32281 ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg gagaaactgg
    32341 aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg tttgtaatcg
    32401 atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt ccggtatttg
    32461 ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg ggtaacgacg
    32521 acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta cgtgaaggcc
    32581 gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag taataaggct
    32641 tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag aattcccggg
    32701 agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg cggcgcgaaa
    32761 tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg ggcctgcata
    32821 tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc acatctccga
    32881 ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg
    32941 cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca tcgagctggc
    33001 aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag gcaacgttgc
    33061 tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc tggaagttaa
    33121 ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag acaaagttct
    33181 ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga
    33241 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc
    33301 gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc
    33361 tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg ctgcaagcaa
    33421 gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag aataccaggt
    33481 acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga aaatggttga
    33541 aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga
    33601 accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat
    33661 ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc
    33721 tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg cttctttttg
    33781 tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg
    33841 tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat ggctaccaat
    33901 gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct gcagggcact
    33961 gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat caaagaactg
    34021 gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct
    34081 ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat gctggcgacc
    34141 gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg
    34201 atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga agctatcagc
    34261 ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc gttctttacc
    34321 accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca
    34381 accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc aaccatgtac
    34441 gagcaattga cttacagcga agtgctggaa aaagagctga aagtcatgga cctggcggcc
    34501 ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa caaaccgggt
    34561 gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga ataattcccg
    34621 tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag tctggttata
    34681 aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg agcgtacaaa
    34741 agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat agcctaataa
    34801 tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact tccactgtgt
    34861 gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat tagcgatatc
    34921 agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac ccaaatcagc
    34981 aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt ggaatattac
    35041 ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc ccgtactctg
    35101 aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat tatggcctcc
    35161 gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct gccgccgctg
    35221 acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga acaagcgcgt
    35281 gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact gttgaaagat
    35341 aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa actgactgat
    35401 gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact gatgcagttc
    35461 tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc tggcggcgtt
    35521 ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca ttctgggctc
    35581 gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg aacacttccg
    35641 cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt gcctggaatt
    35701 ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta aaacgatgct
    35761 acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt gcgatatggc
    35821 agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg ggctgttacc
    35881 tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag aatcactggt
    35941 tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat tgttaccggt
    36001 cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc ataatctggg
    36061 atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt ctggtggccc
    36121 tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag cttgccgtca
    36181 tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga tgaacaaagg
    36241 tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga tggaagtgct
    36301 gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca gtgttctggc
    36361 gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat ggccgaatcg
    36421 cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga catttgccgc
    36481 accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg aacaaggcca
    36541 ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt ttcttgcgca
    36601 acaaatccgc tttacggata tcgctgcgtt gaatttatct gtactggaaa aaatggatat
    36661 gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga gtgaagtcgc
    36721 cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag agtcgcgcta
    36781 tttgttagcg tagggcttca atgatatagt ctgcgccatc tgatcgtaag tagttggctt
    36841 tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct tcagtattcc
    36901 tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc tactcaacca
    36961 cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat ggacggcaat
    37021 ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc cggggcaaaa
    37081 tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaagcgtt aacgctgtat
    37141 gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg
    37201 tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt gcgtctgcgt
    37261 attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg taaatctgaa
    37321 gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta cggtggacgt
    37381 tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa
    37441 ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga actggcccct
    37501 gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt gctttggcaa
    37561 attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga tgaacaagac
    37621 tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg caccgagccc
    37681 ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat atctgctttt
    37741 gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg gttcgccatt
    37801 gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag cggttttacc
    37861 actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc gctgatgctt
    37921 tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat ctcactttgg
    37981 gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc aggttccgca
    38041 gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac cattgttccc
    38101 ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca ttacagtggc
    38161 gcaatatggc tgctctatgt catgatcctg gtatggtgcg ctgactccgg cgcatatatg
    38221 tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg taaaacctgg
    38281 caaggcttta taggtggact cgctactgca gcggtaatct catggggtta tggcatgtgg
    38341 gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc agcgttagcc
    38401 tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat taaggacagc
    38461 ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct gacggctgcg
    38521 gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg gaaggtaata
    38581 tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta cttatcaccg
    38641 tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct
    38701 caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc gaatatgtta
    38761 tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca gaaccggtcg
    38821 ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga gtggcgatta
    38881 ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg ctggttttta
    38941 ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat tcgatagctg
    39001 cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc gaaacgcctg
    39061 attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc accaccatta
    39121 cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta cgtcactggg
    39181 cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct cgtgggccgc
    39241 aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag gcaggtttgc
    39301 aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg
    39361 tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa aggcagggga
    39421 gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa gcgattggtt
    39481 ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt gtacgccagt
    39541 atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg atgaagctga
    39601 cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc
    39661 cgatttctat cgccaagggg gctgggatga cagcggaact cggggtagtt tattacctgc
    39721 cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg ttgcccgtac
    39781 ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga ccggtatccg
    39841 agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg ttaatggggc
    39901 ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac gcataataac
    39961 gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg
    40021 tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg tcgccgttgg
    40081 tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg aagatatcag
    40141 taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg tccttcgtga
    40201 tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca ttactttctc
    40261 cggtaacaaa tcggtgaaag atgacatgct aaagcaaaac ctcgaggctt ctggtgtgcg
    40321 tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc tggaagactt
    40381 ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc cgctgccgcg
    40441 caaccgtgtt gacctaaaac tggtgttccg ggaaggtgtg tcagctgaaa tccagcaaat
    40501 taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt tccaactgcg
    40561 tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac agaaactggc
    40621 gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc gtttcaacat
    40681 cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca cggtgaacat
    40741 caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc ttgccgggca
    40801 ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata acggcactaa
    40861 agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt atgcctatcc
    40921 gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat tacgcgtgaa
    40981 cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta acgatacctc
    41041 gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt tggggagcga
    41101 tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac
    41161 tgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca aggtgaaaga
    41221 acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg gcgtgagctt
    41281 ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg gtatcaacgg
    41341 gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt acttcaccgt
    41401 agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag atgacgccga
    41461 cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct tcccgattaa
    41521 cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt ccaacatgca
    41581 gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga gcacttctga
    41641 tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga cctataacaa
    41701 gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac
    41761 cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga cttatgtgcc
    41821 gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt atggtgatgg
    41881 tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt ccagcaccgt
    41941 gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc atcaggccag
    42001 taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga aagacctgtg
    42061 taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg agttcatcac
    42121 cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat tcttctggga
    42181 tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat atccggacta
    42241 tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga tgtccccatt
    42301 ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag acaaggcaga
    42361 acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg
    42421 tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca aagaactgca
    42481 ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta ttagctgcag
    42541 gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca atcgtcaata
    42601 tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg
    42661 agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag gctaaaatga
    42721 aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa gacgtgatgg
    42781 ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt
    42841 ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa tccgttgcca
    42901 acagccagga tatcgatctg gttgttgatg caaacgctgt tgcttacaac agcagcgatg
    42961 taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct tcaattcgac
    43021 tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat atcgtcatca
    43081 ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg gttaacccaa
    43141 aataccgtga gcatttaggc ttgtgccaag cgtccgcggt tgtcatgacc caggacgatc
    43201 ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact tacgcgcgca
    43261 tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga
    43321 tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg gtgattgagt
    43381 ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca
    43441 gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat gagatccaga
    43501 tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc tttggttatg
    43561 ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt attggcgatc
    43621 gagtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac actgttattg
    43681 gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg attggcgaca
    43741 atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt cgttactgca
    43801 tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa gtgacggtta
    43861 cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc tcaggcattc
    43921 cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca
    43981 tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa cgttccatct
    44041 tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc cgtgttatta
    44101 ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc atactctgca
    44161 gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt
    44221 gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc
    44281 attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga ttctggaagc
    44341 aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga
    44401 gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga
    44461 tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg
    44521 ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg
    44581 ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga
    44641 gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg gaccccatgt
    44701 cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc atactaaaat
    44761 tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc aggatttgaa
    44821 atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt
    44881 caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca gcgacaactt
    44941 actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc
    45001 caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat
    45061 gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg gctgctccgg
    45121 tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa cgccgttcgg
    45181 tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta ccgctatccg
    45241 caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga aaccggaaat
    45301 tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct ttgcacgctc
    45361 aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc cctggtcgcc
    45421 ggagaaatct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg
    45481 cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg ctgcgaagcc
    45541 tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg tcgtctgcgt
    45601 cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa gccagatgtt
    45661 tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct caaaaagcag
    45721 ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca gaaacgcgtt
    45781 ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt
    45841 tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga tgccatgccg
    45901 ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga tgcccactgt
    45961 ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc cgatttcttg
    46021 aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt gccgctggtg
    46081 aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc agacctgtcg
    46141 gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc ggcactactg
    46201 gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt ggtgggatat
    46261 cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga ttatgtctcg
    46321 ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga agagtgtgag
    46381 ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac cagccacgcg
    46441 atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga tgagcaggcg
    46501 gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac acgcagctgg
    46561 ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg
    46621 tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag ctgagcgaaa
    46681 aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg agtctgggcc
    46741 gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg ctggcgatgc
    46801 agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat ggtaaccgct
    46861 gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc gtaccagaaa
    46921 tcagtgccgc gtctatccta gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata
    46981 ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct tttcatctgg
    47041 aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg cctgtcaaac
    47101 gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg aatctgaaga
    47161 tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg atcgatggct
    47221 tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga
    47281 tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga catggcgcag
    47341 ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg ggtgatgagt
    47401 taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg gcgttgctga
    47461 tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat cgcgactggc
    47521 ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc gacgtcggac
    47581 gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt tatgaagaac
    47641 acttcccgga tcgctatttt ctagagctga tccgcaccgg caggccggat gaagaaagct
    47701 atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg gcgactaacg
    47761 atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc gcgatccacg
    47821 acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc
    47881 gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt gccaacaccg
    47941 ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc ctgccgcagt
    48001 tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa gaaggcctgg
    48061 aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc cgcccggaat
    48121 atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggtttc ccgggctact
    48181 tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg gtaggaccag
    48241 gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc gacctcgatc
    48301 cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg
    48361 acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac gtggcggaca
    48421 tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg gcgaaagcgg
    48481 tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac cgtatctcga
    48541 aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc
    48601 tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg gcgcgcaaac
    48661 tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc gcgccgacca
    48721 aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg gtcacccagt
    48781 ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt ggtttgcgta
    48841 cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg
    48901 agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc gacatgctgc
    48961 aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag gacctgatca
    49021 agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg ttccgccctg
    49081 gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt cgtgaagaga
    49141 tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg gagccaacct
    49201 acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt tctggttata
    49261 ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg gaagagatgg
    49321 ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac gccgaactgg
    49381 cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac aaatcgcact
    49441 ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac tatccggcgg
    49501 agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg gtgggcctgg
    49561 tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac tccggtcttt
    49621 accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcc atcaaagggg
    49681 taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc tacttccgcg
    49741 aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg gtgctggaaa
    49801 aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg ctgatgaact
    49861 cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct atcggtcagg
    49921 ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc tacgccagct
    49981 gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta ggcctgtacc
    50041 tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc ggaggcgtaa
    50101 ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacagctgcg gggctcgttg
    50161 ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc acgctggatg
    50221 accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa taccagcaat
    50281 tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat gacttcagcg
    50341 gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg gaaaaatatg
    50401 ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt ttaaaccgac
    50461 tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc tactatcaga
    50521 gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc
    50581 gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg gagtttgact
    50641 aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt gcagagctgg
    50701 aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg gatattaaca
    50761 tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt aaaatcttcg
    50821 ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt ccttataccc
    50881 tagattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac cgtgcgtatg
    50941 cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg gtgatgatca
    51001 ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag
    51061 cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc tttaagatgc
    51121 ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca gaagagcgtg
    51181 gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc gtaccggtag
    51241 tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc gtgggcgata
    51301 aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt tgtgcgtcca
    51361 ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt atcatcgctc
    51421 cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg ggcggcgctc
    51481 accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg gatctggccg
    51541 atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct
    51601 acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc ccttttttat
    51661 cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac acatgaacat
    51721 cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca aagaactgga
    51781 gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg ttgatttgct
    51841 gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg atgagtacag
    51901 tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt atgccttcat
    51961 caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc tctggttttt
    52021 tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt acaccaacga
    52081 atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg tcaaagagcg
    52141 gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa aaagcccggt
    52201 tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg tctctatttc
    52261 ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag cggaagagta
    52321 catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggga catcgacgtc
    52381 gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga tcgaccgcaa
    52441 ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag tctggctgaa
    52501 accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat ttactcgcga
    52561 cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc atgcggtgat
    52621 caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac agacgctgga
    52681 tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc atccaatcta
    52741 ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct tcgaaacgca
    52801 atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca ttaaaggcga
    52861 gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct cgcccagtta
    52921 tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc cgggcaaacg
    52981 gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc agcggctgcg
    53041 ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga
    53101 atgctggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg atgccgatca
    53161 tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg aacagggcaa
    53221 tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg aacgtgggat
    53281 cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg gcatcgataa
    53341 aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt acgatctcaa
    53401 cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt tctaccgcaa
    53461 tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac acgatctttc
    53521 cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc cacatcaggc
    53581 atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac tggtcggtag
    53641 agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga tgccaggaga
    53701 aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt gttccgtagg
    53761 gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg aagacggcgt
    53821 ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt ctgggcgagt
    53881 aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt tcaccatatt
    53941 gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat tcttggtttc
    54001 acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt
    54061 aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg ccccagccga
    54121 ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga tgcggcagtg
    54181 gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa
    54241 aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta tgagcagtaa
    54301 ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt aaaatcgcac
    54361 catcatgaca ctcacgctca atagaccact tctcacctca cgccagattc tggtggcctt
    54421 tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc ggacggaaaa
    54481 tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca atgccgatgc
    54541 ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg tggtcgaacg
    54601 cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg cgcgttatca
    54661 ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac atctcgacga
    54721 tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg ggctttcggc
    54781 tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc ttgcccgcac
    54841 gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg aagacgaaag
    54901 taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc cgttattgca
    54961 gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt gtgctgaaca
    55021 agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa cgtcgcaggg
    55081 gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg cgattattcg
    55141 ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg tgcgcatctg
    55201 gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg gcgcctttga
    55261 aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc aaagcgaaac
    55321 cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc tgggaaccgt
    55381 acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg tcagcgtgcg
    55441 tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta agctgaagaa
    55501 aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac tgctgtttta
    55561 tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg tggctgaagg
    55621 tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag ccggataaga
    55681 cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc accgtaccga
    55741 tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt tcgccagcgg
    55801 cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct tttgcctgca
    55861 atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag gcgagctcaa
    55921 gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac ataatcgctc
    55981 accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga aagggcgtcg
    56041 tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg aacgtatcct
    56101 aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata tttttccaca
    56161 tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta cgaagataaa
    56221 ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc cgcaactgca
    56281 cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg cttaaagcga
    56341 caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt ctcgatgcct
    56401 ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg cgcttgctgg
    56461 cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt gccgatgatg
    56521 agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt gagctggggc
    56581 tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg gcgctgttta
    56641 cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa tgtgtgcagt
    56701 ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta agcgcctttg
    56761 ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg ttatcggatg
    56821 ataagaataa tctggaagtt aacttaaccg cctggtaaca accttcatga ttgtgatttc
    56881 ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg cgcagggcgc
    56941 tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg acattcgggc
    57001 gcccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc atttgatcag
    57061 cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac tgaaccgcga
    57121 agccgctctg gcccggttgg tggcagtgat taaagattta acaaaagaac aaaaagcccg
    57181 acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga aagcacaaaa
    57241 atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa aagaaggaat
    57301 ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt actctgatgg
    57361 gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc gaactgaaac
    57421 cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga atcgaaatct
    57481 ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc ggtgctcgtg
    57541 aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac aagctggtat
    57601 taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg ctggagatgc
    57661 tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa ccggcacaat
    57721 ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg gctgatgcgg
    57781 cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac gcagagctgg
    57841 agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg tcagtagaag
    57901 gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta ttagcgcccg
    57961 atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag taaacctgct
    58021 taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc tacagcatat
    58081 aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta cgccgcatcc
    58141 ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc gacccgcctt
    58201 gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg gcttaaacgc
    58261 agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc ccgattgcgc
    58321 cagtgtaagt ggatgcccgg ctttggcaaa tgcatcagtg acaaattccg aacaaaacca
    58381 cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt cgctgacgca
    58441 ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag tcaccataaa
    58501 gggaataaat tcgacaatgt tgcgatagtt ataaccgcta tctttgattt tattggcaaa
    58561 cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga cgaaaagctt
    58621 atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc ctgtcgcttc
    58681 tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag tgctgaagac
    58741 gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc cgggtttgag
    58801 atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat gctggaattt
    58861 gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg gctggctgat
    58921 atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc agtacgcctt
    58981 tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag gaccctttct
    59041 ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc ttaattaact
    59101 gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg ttacggtcgc
    59161 ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc acgcccggac
    59221 gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg ctgtacagtt
    59281 tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga atcgccacct
    59341 ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc tgctcaatcg
    59401 ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg atttggttac
    59461 ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta cccagctgga
    59521 agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca tccgggcttg
    59581 gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca tcgcgatccc
    59641 agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa tcactcatcg
    59701 ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc agtgaacccg
    59761 gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc gggcttgcaa
    59821 cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc accagcagcg
    59881 caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta accgttttct
    59941 caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc ggcgtatcaa
    60001 ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata gcttctgcca
    60061 gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct tcaccgctct
    60121 gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg tcggcctgta
    60181 cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca tacattgcat
    60241 cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct ttcatcagga
    60301 attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac ttggtctgga
    60361 tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga atcaggtcag
    60421 tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg tcaacaaaac
    60481 gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa tcggctggct
    60541 gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca cgcacgatgt
    60601 tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac ccggaggcca
    60661 gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg gcgtcggcag
    60721 gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta cggttccagt
    60781 tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta gtttaccagt
    60841 gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg caaagacttc
    60901 aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga cggcataggt
    60961 tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca gtacttcgcg
    61021 gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct tttcgacctc
    61081 tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc tggcactggc
    61141 atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg gcgtaccatc
    61201 gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa caacctcttt
    61261 cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg ttgcgaaaac
    61321 ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc agccgccttc
    61381 catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca ggccgcgaac
    61441 ggcgtcggcc tggttgtagg aagcaatgag atgcagttcg ccgttggcgc tttttaccag
    61501 acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa cgcctatttg
    61561 ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat acatacagcc
    61621 tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa tacagcattg
    61681 gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat gctcggcgga
    61741 gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc gcggaacggt
    61801 ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg cggcgctttc
    61861 ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc gacaggggat
    61921 ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg taaagcacgc
    61981 atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct tgtaaaaatg
    62041 acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca gcctaaaaat
    62101 taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta aacttcatca
    62161 gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt
    62221 acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc tttcgccgga
    62281 gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat ttgcgcgtcg
    62341 tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt ttttgggttc
    62401 tcaacaacat caagaactgt cggcagcagg ccaacgccat ctttcagttt gatcaagccc
    62461 actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg cacggcaacc
    62521 tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc agcaatcgga
    62581 taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag ttgctgatca
    62641 aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt
    62701 ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt gtctttcgca
    62761 actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc gactttaatg
    62821 tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc aatcagggct
    62881 cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt caattattta
    62941 tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc tgcgaactga
    63001 attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt cgcgttgtag
    63061 ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac tgcaccaccc
    63121 atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac cagacccggc
    63181 aacgcttccg gtaacagcac tttacggacg atctgcattg gcgtggcacc cattgcgcgg
    63241 gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat acgggcaata
    63301 aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat cgatgtaccg
    63361 acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg gatggaacgg
    63421 aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat aatttgcccc
    63481 ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc aaaaaatccg
    63541 gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca catcatcggc
    63601 tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg caatggcggc
    63661 ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt taacgccacc
    63721 ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac gcgcggtttc
    63781 agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca tcggcacgca
    63841 gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat ccagatgcag
    63901 ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca cttcacttac
    63961 cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac aaatacgctt
    64021 cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc ggttaatgtc
    64081 tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg tggcttcatc
    64141 acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac gttgtttctg
    64201 cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa ccaatgacag
    64261 caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct ccagcggcag
    64321 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
    64381 aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt cctggccatc
    64441 gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta taagcgtact
    64501 cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
    64561 gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg tgatattcga
    64621 aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc tcgaatgcct
    64681 gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
    64741 ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg tcccatgcct
    64801 tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg accgtgatgg
    64861 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
    64921 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg tagtaaccaa
    64981 ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc tgaccgagtg
    65041 gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt attgcccgca
    65101 tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca aaccacatcc
    65161 ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
    65221 gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa caaaagtgct
    65281 ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt gggtgttaaa
    65341 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc aatgattaaa
    65401 agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc gactccctat
    65461 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    65521 aagagaaaaa atcctgaaat tcaggggtga ctctgaaaga ggaaagtgtg atatacgcca
    65581 cctcgcgaca gtgagctgaa agc
//
LOCUS       DAJTRQ010000009        65507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000009 DAJTRQ010000000
VERSION     DAJTRQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            132..575
                     /locus_tag="OO332_000669"
     CDS             132..575
                     /locus_tag="OO332_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5941260.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="OO332_000670"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="OO332_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO332_000671"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO332_000671"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5941261.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO332_000672"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO332_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5941262.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO332_000673"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO332_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5941263.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO332_000674"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO332_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5941264.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OO332_000675"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OO332_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5941265.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OO332_000676"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OO332_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5941266.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OO332_000677"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OO332_000677"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5941267.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OO332_000678"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OO332_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5941268.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OO332_000679"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OO332_000679"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5941269.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OO332_000680"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OO332_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5941270.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OO332_000681"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OO332_000681"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5941271.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO332_000682"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO332_000682"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5941272.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="OO332_000683"
     CDS             13936..14316
                     /locus_tag="OO332_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5941273.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OO332_000684"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OO332_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5941274.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO332_000685"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO332_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5941275.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO332_000686"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO332_000686"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5941276.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(17417..17492)
                     /locus_tag="OO332_000687"
     tRNA            complement(17417..17492)
                     /locus_tag="OO332_000687"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OO332_000688"
     tRNA            complement(17496..17571)
                     /locus_tag="OO332_000688"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OO332_000689"
     tRNA            complement(17623..17698)
                     /locus_tag="OO332_000689"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OO332_000690"
     tRNA            complement(17701..17776)
                     /locus_tag="OO332_000690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="OO332_000691"
     tRNA            complement(17913..17988)
                     /locus_tag="OO332_000691"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO332_000692"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO332_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5941277.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO332_000693"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO332_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5941278.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO332_000694"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO332_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5941279.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO332_000695"
     CDS             complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO332_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5941280.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO332_000696"
     CDS             complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO332_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5941281.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO332_000697"
     CDS             complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO332_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5941282.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO332_000698"
     CDS             complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO332_000698"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5941283.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO332_000699"
     CDS             complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO332_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5941284.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO332_000700"
     CDS             complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO332_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5941285.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO332_000701"
     CDS             complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO332_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5941286.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO332_000702"
     CDS             complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO332_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5941287.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO332_000703"
     CDS             complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO332_000703"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5941288.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO332_000704"
     CDS             complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO332_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5941289.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAIDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            32661..33383
                     /gene="mngR"
                     /locus_tag="OO332_000705"
     CDS             32661..33383
                     /gene="mngR"
                     /locus_tag="OO332_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5941290.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO332_000706"
     CDS             complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO332_000706"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5941291.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO332_000707"
     CDS             complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO332_000707"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5941292.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO332_000708"
     CDS             complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO332_000708"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5941293.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO332_000709"
     CDS             complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO332_000709"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5941294.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO332_000710"
     CDS             complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO332_000710"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5941295.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO332_000711"
     CDS             complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO332_000711"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5941296.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO332_000712"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO332_000712"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5941297.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO332_000713"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO332_000713"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5941298.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43574..43681
                     /locus_tag="OO332_000714"
     CDS             43574..43681
                     /locus_tag="OO332_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941299.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43887..45170
                     /gene="gltA"
                     /locus_tag="OO332_000715"
     CDS             43887..45170
                     /gene="gltA"
                     /locus_tag="OO332_000715"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5941300.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45561..46127
                     /locus_tag="OO332_000716"
     CDS             45561..46127
                     /locus_tag="OO332_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5941301.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46178..48637
                     /locus_tag="OO332_000717"
     CDS             46178..48637
                     /locus_tag="OO332_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5941302.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48652..49383
                     /locus_tag="OO332_000718"
     CDS             48652..49383
                     /locus_tag="OO332_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5941303.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49380..50442
                     /locus_tag="OO332_000719"
                     /pseudo
     CDS             49380..50442
                     /locus_tag="OO332_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50594..51640
                     /locus_tag="OO332_000720"
     CDS             50594..51640
                     /locus_tag="OO332_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5941304.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO332_000721"
     CDS             complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO332_000721"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5941305.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO332_000722"
     CDS             complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO332_000722"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5941306.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO332_000723"
     CDS             complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO332_000723"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5941307.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO332_000724"
     CDS             complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO332_000724"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5941308.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO332_000725"
     CDS             complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO332_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5941309.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55807..57288
                     /gene="dtpD"
                     /locus_tag="OO332_000726"
     CDS             55807..57288
                     /gene="dtpD"
                     /locus_tag="OO332_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5941310.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO332_000727"
     CDS             complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO332_000727"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5941311.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(58853..59362)
                     /locus_tag="OO332_000728"
     CDS             complement(58853..59362)
                     /locus_tag="OO332_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5941312.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59491..59808
                     /locus_tag="OO332_000729"
     CDS             59491..59808
                     /locus_tag="OO332_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5941313.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(59863..60243)
                     /locus_tag="OO332_000730"
     CDS             complement(59863..60243)
                     /locus_tag="OO332_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941314.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(60246..64910)
                     /locus_tag="OO332_000731"
     CDS             complement(60246..64910)
                     /locus_tag="OO332_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5941315.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
     gene            complement(64929..65132)
                     /locus_tag="OO332_000732"
                     /pseudo
     CDS             complement(64929..65132)
                     /locus_tag="OO332_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15871 a        17746 c        15995 g        15895 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacatttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgttttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gctgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccag cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt atcagctgca gccttttcag cagccgcttt
    21541 cttctcagct tctgccttgg ctttttcagc agcttcagtt gccgctttct ttcttgcttc
    21601 agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg ctacctcggc
    21661 ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt tcttcgcgtc
    21721 tgcagccgct ttcttcgcag cttcttccgc agctttagca tctgccgccg ctttcgctgc
    21781 cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
    21841 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
    21901 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
    21961 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
    22021 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
    22081 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
    22141 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
    22201 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
    22261 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
    22321 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
    22381 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
    22441 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
    22501 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
    22561 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
    22621 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
    22681 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
    22741 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgagac
    22801 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc gcaatcaacg
    22861 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
    22921 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
    22981 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
    23041 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
    23101 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
    23161 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
    23221 cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc tggataataa
    23281 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
    23341 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
    23401 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
    23461 tgcttcattc agcaaagtat tctcggcgtt gacaatatgt tgcgtgaaaa ccaaagaggt
    23521 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
    23581 ataatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
    23641 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
    23701 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
    23761 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
    23821 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
    23881 aaaatttatg ggcctagagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
    23941 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
    24001 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
    24061 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
    24121 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
    24181 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
    24241 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
    24301 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
    24361 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
    24421 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
    24481 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
    24541 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
    24601 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
    24661 cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
    24721 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
    24781 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
    24841 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
    24901 gtgcagttcg cccccggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
    24961 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
    25021 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
    25081 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
    25141 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
    25201 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
    25261 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
    25321 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
    25381 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
    25441 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
    25501 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
    25561 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
    25621 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
    25681 tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
    25741 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
    25801 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
    25861 cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg caaagtacag
    25921 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
    25981 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    26041 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    26101 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    26161 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    26221 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa aggcagcagg
    26281 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    26341 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    26401 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    26461 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    26521 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    26581 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaat
    26641 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    26701 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    26761 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    26821 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    26881 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    26941 tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca taccgagcgt
    27001 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    27061 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    27121 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    27181 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    27241 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    27301 aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc agccaatttg
    27361 atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat tgataaatat
    27421 caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga catttatgtt
    27481 taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag ataaaaagag
    27541 acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc taatacgacg
    27601 cagagtttca ggattattaa taaaggcata aatctcctcc ccgccgatat taacatttaa
    27661 ttaacttata attatattat aattaactca cccggggtta ttattttgtt aaaaataaag
    27721 ccacatatac gtagcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
    27781 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
    27841 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
    27901 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
    27961 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
    28021 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
    28081 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
    28141 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
    28201 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
    28261 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
    28321 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
    28381 ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag taagcccacg
    28441 ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
    28501 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
    28561 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
    28621 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
    28681 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
    28741 atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc gacacctact
    28801 ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc catttcatag
    28861 cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg tttcgcattc
    28921 gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
    28981 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
    29041 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
    29101 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
    29161 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
    29221 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
    29281 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
    29341 cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa caggacgagt
    29401 ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa acgcagcaga
    29461 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
    29521 ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt ccatattttc
    29581 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc
    29641 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc catgcgcgtg
    29701 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
    29761 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
    29821 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
    29881 aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
    29941 agcacgtcaa aataactgtc gaggcgtttg cggaatccgt tttcgtccgc aggtaagtac
    30001 ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc gtcactgctt
    30061 tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg ccagaacatg
    30121 gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
    30181 tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
    30241 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
    30301 gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt gttttccggt
    30361 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
    30421 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
    30481 gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
    30541 cgagataccg ctttcatcgt cgtttccttt ttgtttattg cattacgcca tcagtcagat
    30601 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
    30661 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
    30721 catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg atattcatcg
    30781 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
    30841 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
    30901 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
    30961 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
    31021 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
    31081 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
    31141 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
    31201 acagcatcag actgcccgct cccaatgtac cgagcaccag gtagagataa aaagtcagga
    31261 agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc aagtaaccgg
    31321 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
    31381 cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg gtataggctg
    31441 ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg tacatccaca
    31501 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
    31561 cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggaaaaagag atcccgctca
    31621 acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct tgctgtacgg
    31681 tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct tctgcatggc
    31741 gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc tcactctcct
    31801 tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga tccgccgtta
    31861 aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc
    31921 gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
    31981 ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
    32041 tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
    32101 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg
    32161 cttcattggg gggaatcgcc agcaacacca ctaaatcaat cgcttccggg ccatccacgc
    32221 tttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
    32281 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
    32341 ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac
    32401 gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
    32461 catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt
    32521 cgcgccagtg tgcccgataa aacaatacca ttgcgtaatc aatttcactt gattgaaatt
    32581 atttgcattg tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg
    32641 tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt gctgatcgca
    32701 ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct accgaatcgg
    32761 cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag
    32821 tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa gaagagcggg
    32881 tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg
    32941 atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt cttcagcaac
    33001 agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc
    33061 aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct
    33121 ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag atggttatcg
    33181 atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc cgtctgctta
    33241 atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta gttgatggtc
    33301 gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt accctgatag
    33361 cgcagcgaaa atcatcgcga taagcacaaa aaaggccatc atacgatggc caaccatgtc
    33421 gaaaccgaca gcccttcgcc attcctgtag agggaagcgg cgagggctat ttcttattac
    33481 agatatttat ttcagaacag ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt
    33541 tttcacgcct gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc
    33601 aatgatcgca cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta
    33661 acccacaact ggcttggtaa catgctcttt gatgtacgct gccgcttctt cttcagcgct
    33721 accgccgatc tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc
    33781 gagaatgtcg ataaagttag agccagggat cgggtcaccg ccgataccga cacaggtcga
    33841 ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca taggtcagtg taccggaacg
    33901 ggaaacgata cccactttac ccggtttgtg aatgtgaccc ggctggatac cgattttgca
    33961 ttcacccggg gtgataacgc ctgggcagtt cgggccgatc atacgaacgc ctgcttcgtc
    34021 cagtttcact ttcacggtca gcatatccag cgtcgggatg ccttcagtga tggtgataat
    34081 cagtttgatg cctgcatcga tagcttccag aatggagtct ttgcagaacg gtgctggtac
    34141 gtaaataaca gacgcggaag cgccagtagc agcaacagct tcacgcacgg tgttgaacac
    34201 cggcaggccg aggtgggtag tgccgccttt acctggagtt acgccaccaa ccattttagt
    34261 gccgtatgca atggcctgtt ctgagtggaa agtcccctgg ctaccggtaa agccctggca
    34321 gataaccttg gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca
    34381 acctgctgag ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc
    34441 agtttcttcg cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta
    34501 acacccactt ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata
    34561 ccgccgaaga tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaac
    34621 gcttcggtta cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agccggttcg
    34681 ccgccgtgca gtttaactat gtccatcgta cccatcgcca gacctgcgcc gttaaccata
    34741 caaccgatgt taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca
    34801 cgcggatctt cctgcgactg gtcacgcatt tcgcgcagat caggctggcg gaacagggcg
    34861 ttgccatcag cgcccagttt gccgtcgagg caaatcagat cgccctgttt ggtgatgacc
    34921 agcgggttga tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg
    34981 aagattttgg tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg
    35041 cgtccctgat acggcatcgg gccagtcagc ggatcaagcg caactttatg gatcaggtgc
    35101 ggagtttctt ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc
    35161 acgcgacggg aactacggtc tacaacggca ccgagataca gctctttagc gatatcggtc
    35221 gccgcttcaa ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc
    35281 agacgcttgc ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttacaact
    35341 ttcacaccgc ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga
    35401 ccggcaccga tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc
    35461 accggtgctg gtaagccata gcgggcaaac agttgttttg cctgatattc atgtaagttc
    35521 atgtgttctg tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc
    35581 cacgtcggtc tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgcgtc
    35641 ggctcttcca gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc
    35701 agacggtgat cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc
    35761 accgccatcg gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggcgggttg
    35821 atgatcggcg tagacatcag ggaaccgaac acaccaccgt tggtgatggt gaagttacca
    35881 ccggtcagat cttcaaccgt cagcttgcca tcacggcctt tgactgccag ctctttgatt
    35941 ttcttctcga tgtctgccat gccgagggta tcgacatcac gcagaaccgg cgtcaccagg
    36001 ccgcgcggcg tagaaaccgc catgctgacg tcgaaatagt tgtggtaaac cacgtcatcg
    36061 ccgtcgatag acgcgttcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg
    36121 tagaaggaca taaagcccag acggatgccg tggcgttttt caaacgcttc accgtactgc
    36181 ttacgcagat ccataatcgg cttcatgttg acttcgttga acgtggtcag catggcggtg
    36241 gagtttttcg cttccagcag acgctctgcc acacgcttac gcaggcgagt catcgggaca
    36301 cgtttttcac tacgtgcagc cagagccggt tgcgccgccg gagcagccgc tgccggagca
    36361 gactctttcg ccggggcttt cgccagatgt ttttccacat cttcacgagt cagacgacca
    36421 cccacaccgg tgcctttaat ggcgctggcg tcgagattgt gttcagccag caggcgacgg
    36481 atcgccgggc ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc
    36541 ggagtggacg ctttctcttc agatttggcg ctggtttctt taccggcgct gttgccttca
    36601 cgcaggcgac caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca
    36661 tccagaatgc cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct
    36721 accagcactt catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg
    36781 gcatcggcta cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat
    36841 cctttattta ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa
    36901 cggacatata ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag
    36961 ccccaaacgg aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga
    37021 gcggctcttc ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct
    37081 gcatcgcttt atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt
    37141 gattgttctt acgacgctgt tccagcaggt cgtaataaac cttaccagaa cacatcacta
    37201 cgcgcttcac gcccttcgga tcaagttcgt cgatttcacc gatggctggc aggaaggtgc
    37261 cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg gatttcggcg
    37321 acatcacgac cagcggacga cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa
    37381 cctgtgccgg ggtagacggt acgcaaacct gcatgttttg ctcagcacaa agttgcagat
    37441 aacgttccag acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca
    37501 acatcaccag accacacatc cggccccatt tctgctcgcc agaggagatg aactggtcga
    37561 taaccacctg cgcaccgttg gcgaagtcac cgaactgcgc ttcccagatg gtcagggtgc
    37621 gtggttctgc ggtggcataa ccatattcaa acgccagcac ggcttcttct gacagtacgg
    37681 agtcccagac acggaacgcg ccctgaccgt tatggatgtg ttgcagcggc gtgtaagtgg
    37741 aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt
    37801 cttcacccga caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt
    37861 ccgcaccgcc ccagtcgaac agtttctcac cggctgccat cgcctggcga tcgccataaa
    37921 tcttggcaac gcgagactgc atttcaactg cttctggtac cgtactgata cgtttagcca
    37981 gttcctgcag gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga
    38041 ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg ccactctgcc actacgcaat
    38101 cgccagcatc cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg
    38161 ccactttttc ctgctccagc ttgtcagcgt agattttgcg cggtgtcgga tgttttttga
    38221 ttttctgata catcagtggc tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac
    38281 ggcggtagca taccaggtcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg
    38341 ccagacgggt cacaaaggca acggcttccg gatcatccgc gttaacgtgg aaaatcggtg
    38401 cctgaaccat cttaccgata tcagtacagt acggtgtaga acgggcatcc agcgggttag
    38461 aggtggtgaa accaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac
    38521 cacgcgcttt cgacatgttc agagtttcct gaaccacgcc ctgcccggtc actgcggcgt
    38581 caccgtggat ggtgattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac
    38641 gggcacgaac agagccgata acgaccgggc ttacaatctc aaggtgagac gggttaaacg
    38701 ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt
    38761 atttcacgtc gcctgtgccg aggtgttctt tatgtttacc ggcgaactcg tcgaacaagt
    38821 cttgcggttt tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc
    38881 cgagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg
    38941 ggattaacgc gtcaccgcct tccagcgaga agcgttttgc gccagggaat tttgcgccga
    39001 ggtaacgctc aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc
    39061 tattgaaagt cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcggtgc
    39121 tggtgatgtg catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcggaaa
    39181 taagctcgcc gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggttt
    39241 cctggaagtc tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt
    39301 gctgccacag tcccagcgga tctagattcg catgctggtg accacggaag cggtatgcgt
    39361 taatgagctg caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag
    39421 agtaacgtga agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt
    39481 gatccggttt gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa
    39541 ccgagtcagg atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc
    39601 cagagaggta agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt
    39661 aagcatcttt tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc
    39721 ggggttcact tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt
    39781 cttagtgtca ggggcagttt ttaaagattt cctgcatttc tttgttacgc ctgatgcgct
    39841 gcgcttatca ggcctgcggg taatgacttg taggccggat aaggcgttca cgccgcatcc
    39901 ggcactggtt gcctgatgca acgcttgcgc gtcttatcag gcctacagtt tacgcattac
    39961 gttgcaacaa catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac
    40021 atacactgac gcagttcatg atgctgtgac agcggaatac gctgaatgca tcgctcaaac
    40081 cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta
    40141 acaagcctgc cgggccgata aacttatcgg gattccacca gaaagacgga caagaggttg
    40201 aacaacatgc gcagagaata cattcataca gcccgtcgag tttttcgcgc tgctctggca
    40261 tctgtaaatg ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt
    40321 tctcatattg cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac
    40381 ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa
    40441 tacaggccag accattcttg ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac
    40501 ggcaggagcg gcggaacgac aggctgggat ctttctcttt cagctggata agcgcatcca
    40561 gcagcatcat gtcgcgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat
    40621 catcaacatc cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc
    40681 attagtaagt acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc
    40741 ttcggcgcgt catggattcc gactcaggca gatacaggga gtggcacagc cagttttcat
    40801 catcacgatc cgggaagtcg aagcggctat gtgcgccacg gctttcggta cggaagttgg
    40861 cagaaacagc cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc
    40921 gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc atttttcaga cgctcgcgga
    40981 tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt
    41041 tatgctgcat acattcttgc agcgctttac ggatcgccac cggatcttca ccgttacgat
    41101 tattgttcca ccggttcagg cgatccagag acgcttcaac atcagactcg ctggcatcgc
    41161 gcagtgcgcc ctgctcggcg atagactctt gcagatgcag acctgccgcg cgaccaaaga
    41221 ccaccaggtc cagcagcgag ttgccgccca gacggttagc gccgtgtacc gatacacaag
    41281 cgatttcacc aacggcaaac agtcccggaa caaccacatc ttcgcctttc tcattcacag
    41341 tcagcgcctg accggtaact ttggtcggaa taccgcccat catgtagtga caggttggga
    41401 taaccggaat cggctctttc accggatcga cgtgagcgaa ggtacgggaa agctccagga
    41461 tacccggcag acgggattcg agaacttctt tacccaggtg atcgagtttc agtttcgcgt
    41521 gtggccccca cggaccatca cagccgcgac cttcacggat ttcaatcatg atggaacgcg
    41581 ccaccacatc acggcccgcc aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt
    41641 cgccatgttt gttcagcaga taaccgcctt caccacggca accttcggtg accagtacgc
    41701 ccgcaccggc aatgccggtc gggtggaatt gccacatttc catatcctgc accggtacgc
    41761 ctgcgcggat agccatgccg acaccgtcgc cagtgttaat gtgggcgtta gtggtggact
    41821 gataaatacg ccctgccccg ccagtcgcca gcacggtagc gcgggctttg aaataaacca
    41881 cttcaccggt ttcgatgcac agtgcggtac aacccaccac cgcgccatcc tggtttttca
    41941 ccagatccag cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat
    42001 aaagcgtgtg caacagtgcg tgaccggtac ggtcagcagc cgccgcagtg cgtgccgcct
    42061 gctcgccgcc gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat
    42121 cgagacgcga gaacggcagg cccatgtgtt cgagttccag aatcgcttcc ggcccggttt
    42181 tacacatata ttcaatcgcg tcctggtcac cgatatagtc cgaccctttt acggtgtcgt
    42241 acatatgcca ttcccagtta tcttcatggg tattacccag cgcaacggta atgccgcctt
    42301 gcgcagaaac ggtatgggaa cgggtcggga agactttaga gagcagcgca caggtctggc
    42361 cgctctggga aatttgcagc gccgcgcgca tacctgcgcc accggcacca atcacaactg
    42421 catcaaattc tctgactggc aatttcatca caccccccac accacaacga atccataaat
    42481 cacgtaaacc accagtgcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg
    42541 tttaacgtag tcggtcaaca cctgccacat gccgatccag gcatgaatca agatagaaaa
    42601 cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg aagaaaccga tccagacttc
    42661 atatgtcagt tcgccactgg tggcgaaaaa accgaccata taaatgatgt agagcgtcag
    42721 gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga
    42781 ggcgttgctt accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag
    42841 gagattttgg cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc
    42901 atcatgtggc gaatacctac gacgacgtga tacgccagag cggtaaggat gccccacatg
    42961 ataaatttga cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa
    43021 gagaggctgg tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg
    43081 gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga accggatggt ctgtaggtcc
    43141 agattaacag gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc
    43201 ttattattcc ctggggacta cgggcacaga ggttaacttt ctgttacctg gagacgtcgg
    43261 gatttccttc ctccggtctg cttgcgggtc agacagcgtc ctttccataa ctgcgcgtca
    43321 tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt
    43381 gcagggtgtt ccggagacct ggcggcagta taggctgttc acaaaatcat tacaattaac
    43441 ctacatatag tttgtcgggt tttatcctga acagtgatcc aggtcacgat aacaacattt
    43501 atttaatttt taatcatcta atttgacaat cattcaacaa agttgttaca aacattacca
    43561 ggaaaagcat ataatgcgta aaagttatga agtcggtatt tcacctaaga ttaacttatg
    43621 taacagtgtg gaagtattga ccaattcatt cgggacagtt attagtggta gacaagttta
    43681 ataattcgga ttgctaagta cttgattcgc catttattcg tcatcaatgg attctttacc
    43741 tgcaagcgcc cagagctctg tacccaggtt ttcccctctt tcacagagcg gcgagccaaa
    43801 taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac
    43861 gcaataaggc gctaaggaga ccttaaatgg ctgatacaaa agcaaaactc accctcaacg
    43921 gggacacagc tgttgaactg gatgtgctga aaggcacgct gggtcaagat gttattgata
    43981 tccgtactct cggttcaaaa ggtgtgttca cctttgaccc aggcttcact tcaaccgcat
    44041 cctgcgaatc taaaattact tttattgatg gtgatgaagg tattttgctg caccgcggtt
    44101 tcccgatcga tcagctggcg accgattcta actacctaga agtttgttac atcctgctga
    44161 atggtgaaaa accgactcag gaacagtatg acgaatttaa aactacggtg acccgtcata
    44221 ccatgatcca cgagcagatt acccgtctgt tccacgcttt ccgtcgcgac tcacatccaa
    44281 tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt ctatcacgac tcgctggatg
    44341 ttaacaatcc tcgtcatcgt gaaattgccg cgttccgcct gctgtcgaaa atgccgacca
    44401 tggccgcgat gtgttacaag tattccattg gtcagccatt tgtttatccg cgcaacgatc
    44461 tctcctatgc cggtaacttc ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag
    44521 ttaatccgat tctggaacgt gctatggacc gtattctgat cctgcacgct gaccatgaac
    44581 agaacgcctc tacctccacc gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct
    44641 gtatcgcagc aggtattgct tcactgtggg gacctgcgca cggtggtgct aacgaagcgg
    44701 cgctgaaaat gctggaagaa attagctccg ttaaacacat tccggaattt gttcgtcgtg
    44761 cgaaagataa aaatgattct ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt
    44821 acgacccgcg cgccaccgta atgcgtgaaa cctgccatga agttctgaaa gagctgggca
    44881 ccaaagatga cctgctggaa gtggctatgg agctggaaaa catcgcgctg aacgacccgt
    44941 actttatcga gaagaaactg tacccgaacg tcgatttcta ctctggtatc atcctgaaag
    45001 cgatgggtat tccgtcttcc atgttcaccg tcattttcgc aatggcacgt accgttggct
    45061 ggatcgccca ctggagcgaa atgcacagtt acggtatgaa gattgcccgt ccgcgtcagc
    45121 tgtatacagg atatgaaaaa cgcgacttta aaagcgatat caagcgttaa tggttgattg
    45181 ctaagttgta aatattttaa cccgccgttc aaatggcggg ttaattttta tatgcctaaa
    45241 cacaaaaaat tgtaaaaata aaatccacta tcggacattc atatattcta aaaaaaaata
    45301 tatcggtata aattataaac aaatcgatga tttttaatca tctatcttcc tctcagttac
    45361 atgaataaac caccaaatat agcgcacccc tataatttcc aatactttat tcatcaaaca
    45421 tttaattcat accatcaatt aattactttt acatttaata aaattaataa tatttttaag
    45481 aaaaactaaa aacattcgac aatcaaataa gatattcttg caccgatata cttttttctt
    45541 ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac attggccgca ctggccgtat
    45601 ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa attcacttta
    45661 aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat aaagaggttg
    45721 aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat gccgttgctg
    45781 tcgacctgct cttagtcaac tgtgatctgg aaaactccag caacggttcc ggtggcaaga
    45841 tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca gatccgattc
    45901 tcaacaacac cagcacgggt gaagccacct gcgtcggcgt acgtttaatg aataaagatc
    45961 aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg acctccagcg
    46021 aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca cctgtaacgc
    46081 caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta ttactttggc
    46141 ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga atatttatcg
    46201 actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgc tcgaattcaa
    46261 tctgaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat taaaggaaaa
    46321 aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata accaaatcag
    46381 taacgagcaa aagattaact ggcacaaaaa tgacgataaa accattccgt gcatcaatga
    46441 tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcattac cattgataaa
    46501 tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc aagccaatca
    46561 gcaactgaat attagtattc cgcaagcctg gctggcgtgg tactcagaaa actggacccc
    46621 accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact tgtttgccag
    46681 cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg gaacagccgg
    46741 aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcata ctgatagcga
    46801 tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc cgttaccgca
    46861 attaggttca aaattaaccc tcggcgaaac ggattttagt tccaatattt tcgacggttt
    46921 ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg aactacgcgg
    46981 ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga tcagtcaatc
    47041 aggccgcgtc atttaccaga aaaaagtccc accaggccca tttatcattg acgaccttaa
    47101 tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa
    47161 tttccaggtt tcagcagcat cgacgccctt cctgactcgt cagggacagg ttcgctataa
    47221 actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg aaaccttttt
    47281 tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacagcg gcctgctgct
    47341 ttctggtgat gactaccatt ctgccgcaat gggtattggg caaaatatgc tgtggcttgg
    47401 tgcgctgtcg tttgatgtca cgtgggccag tagccatttt gatactcagc aggacgagcg
    47461 gggcttaagc taccgtttta attacagcaa acaagtggat gccactaaca gcacgatttc
    47521 cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact atctggatca
    47581 caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat ctgtgggcca
    47641 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
    47701 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
    47761 gagagatatc tctatttcta cgtcattcaa tacaacccat tacgaagata aagatcgcga
    47821 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcagg ttggctatga
    47881 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
    47941 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca atggagccca
    48001 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
    48061 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
    48121 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
    48181 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
    48241 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
    48301 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
    48361 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
    48421 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
    48481 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
    48541 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
    48601 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
    48661 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
    48721 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
    48781 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
    48841 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
    48901 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
    48961 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
    49021 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
    49081 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
    49141 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
    49201 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
    49261 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
    49321 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
    49381 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
    49441 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
    49501 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgcatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccttgagca gatcatcgcg atgggatggc
    49801 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
    49861 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
    49921 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
    49981 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
    50041 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
    50101 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
    50161 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
    50221 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
    50281 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac atgttggtct
    50341 acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc gaggctgggg
    50401 cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt
    50461 gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct
    50521 gttatttttt cctagcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat
    50581 gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct
    50641 ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc
    50701 cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc
    50761 cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac
    50821 gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc
    50881 catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg
    50941 ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat
    51001 tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc gtcgcgggtg ccgctgttct
    51061 ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt
    51121 cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc
    51181 gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt
    51241 gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta
    51301 tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact
    51361 acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat
    51421 ggcgtgggga ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag
    51481 tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct
    51541 tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacagggc cagccattgc
    51601 gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca
    51661 gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag
    51721 ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc
    51781 cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc
    51841 atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca
    51901 gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa
    51961 tcctgcaaac tgacggttac gaaagcgcgg cgacaataac cgttccttta ccacctccgg
    52021 cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag
    52081 ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc
    52141 cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac
    52201 gcgccagacg ccgtaaagct gattatggct gtagagcgtt aaaccgttgg gaaaatgagt
    52261 taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg
    52321 ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc
    52381 cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt
    52441 ttttcattct ttattaagcg gggttatgct gcgacaacaa tccccttttt ggcaaatgta
    52501 gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag gcagaccgtt
    52561 tgcgccgcga ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc
    52621 tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc
    52681 ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc
    52741 aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat
    52801 gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc
    52861 gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg
    52921 gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttccggcggc
    52981 agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc
    53041 gcgacaccat tttttattgc ttcacgcacg caagcctgca tggtttgcgc atcgcctgca
    53101 tgaaatccac aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg
    53161 cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc acgctaattg
    53221 ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac
    53281 aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat
    53341 acaggcaata cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca caatcggctg
    53401 cccgttatgc ggcacctgca ccacgcccgg taacaaaccg tgagataaca gttcgcgatc
    53461 ggtgatgcgt tttaaaattt gcccctgtaa acgatagccc attcggttac tttgcgagct
    53521 aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc
    53581 cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc
    53641 cataaaatca cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc
    53701 ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac
    53761 atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt
    53821 taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcgt tatcatccaa
    53881 ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca tcagtttcga actcaactgt
    53941 taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt
    54001 agcaatgcgc agcgcaggca tatccagtgc gccacagtgg ctgatacccg actggcgaaa
    54061 accgtgacga ccgccatcct gcacagtggt gtacatgccc gcacgaataa tcttcagcat
    54121 attccctcct tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa gatgggttcg
    54181 tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc
    54241 gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg
    54301 agcaagcgcg gttcagcgcg ccgtggcgtg tgttgttgtt ccggcaaact cccgagatac
    54361 gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca
    54421 acaacctgtt tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc cggtccgcct
    54481 gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc
    54541 tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc
    54601 aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc
    54661 agacgctgcg ccagtcgcca gatccgcttc tggctagcca gcgtcaccgg cggttccagt
    54721 tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc
    54781 tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt
    54841 ttcattcagc cactcgctca atgcgcgaat accaccgcgt tcagtggcat ggtgacctgc
    54901 agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc
    54961 agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc
    55021 cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt
    55081 acgtcccaga cgcgcttcaa tccaggaggc tagttccatt cccggcacag gcatggtcag
    55141 ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc
    55201 cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag
    55261 gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg
    55321 agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac
    55381 tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc
    55441 tttgccttcc acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt
    55501 ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata
    55561 gctacaccgt taacatagcg ttacctcgcc tgttcgtcga tagccaatct tttgacatat
    55621 taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat
    55681 gtatatttat gcaatcagac agcatattgc ataaataatc agttctgtat caaagtttta
    55741 actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga
    55801 attattatga ataaacacgc atcacaaccg cgcgctattt actatgtcgt tgcgctgcaa
    55861 atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat
    55921 caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg
    55981 tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg
    56041 gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc
    56101 catccgtcat tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa
    56161 tctaacgtaa gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc
    56221 ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc tatcgcctgt
    56281 ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg
    56341 atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt
    56401 aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt
    56461 ctgctggtgg caacgcctgc actgattacc gtgctgttct ggaaagagtg gtcggtatac
    56521 gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca
    56581 gaaaaccaga aacagcggaa ggagctgggg ctgattgtga cgctcacctt attcagtatg
    56641 ttgttctggg ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc
    56701 gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc
    56761 ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt
    56821 aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga
    56881 ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca
    56941 ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc
    57001 atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac
    57061 atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg
    57121 caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta
    57181 tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg
    57241 tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc
    57301 gatgcctgat gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg
    57361 aatttgcaca aatttgtagg ctggattagg cgttcacgcc gcatccggca tctgggctcg
    57421 attgcctgac gcgtctgtta tttccccttc cgcgccgtct catacgctgc caacgtttgt
    57481 actctcgcct ctttgtgctc gactatcggt tgcggataat ccagcttcac acctgctttc
    57541 tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac
    57601 catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgagttat aggattgaaa
    57661 atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta
    57721 ttagctgcca aatcaccatc aatcagctgc gacataaaat atcgctcgcc ttcgcgccag
    57781 tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc
    57841 cagccagtgc tgttaagctg acgcatagcg gcatcaataa tcgggtatcc cgttttgcct
    57901 ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc cgtccaggca
    57961 ataaatggac gatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg
    58021 cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt
    58081 tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc caagctggcg
    58141 gacaaacggc tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccagca
    58201 ccgttctcgc aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc
    58261 gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat
    58321 ccgctactac gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc
    58381 agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc ggtcatcacc
    58441 gcccccggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct
    58501 ctttcaacgt ccacatcccg cgctcgctca ttcacctcat actgatagtt ataaaaaaga
    58561 tgagtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca
    58621 tccacttcac ggaacaataa aggaatacct ttttccgcca gcgcgatttg tagcccattc
    58681 agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtctc ccactggcgt
    58741 ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt
    58801 gcgagattat cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt agtcataaaa
    58861 ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa
    58921 gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg
    58981 gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat
    59041 caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt
    59101 tggcgggatg agaaagcaaa acaattacgc gttggcggta ctggttgtaa aaatcatcca
    59161 gattttgcca ctgatgaata tcagccacaa acgggccaag tttgcggtac tcgggctgag
    59221 aatgggccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta
    59281 agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt
    59341 catcaaatcg ttgtagattc attactgctc ctgaacggta tacaggagta agtgtagacg
    59401 ttggtaaatg atgtttgtga agttaatccg ctagcagggc aagattacga aagcgcgctc
    59461 cccgcaagca actaatcaag gtagttaatc atggctgaac acgatattac gccagaagtc
    59521 accatttcca aaacacagcg ccgctataaa gtcggctatg tcagagcccg ccaccaggac
    59581 cgcaaaaccg gtatagcccg gtattatagc ctgcacctta agggcgactg gctgaaagag
    59641 gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc
    59701 atcgctgaca gtgacgaggt gcaggaactg cgggaggaac tttatcaggt taaacaggta
    59761 gttaagggga tgcgggatgg gatgtttagt gtgctgaacg aaagttaaat attaaaaaag
    59821 ctgggaagat ttaaaacctt cctagctttt tattctgtct tattataatg tatactcatc
    59881 atcttccgat gaatttataa attgctccaa gtttacatga agagtactta acctagtttt
    59941 taactcatta agtgaagcga taatttcata ttttccaaca taatcatatg atggatcgta
    60001 ttcattatct atgtcagaag ctaaaaatga tgatattcgt agtttaaatt tagcttcatc
    60061 tttattacat tcattggcat aaggtgaatg ccctatcata gcatattcct ctaatctaaa
    60121 aggataagat ggttttaatt catcgatcaa tgaaatttca atccatgttc tatatccagg
    60181 cattaatgga ctaatttctt tgagcaaaac ctttccactt aataatgtat tgagattgtt
    60241 cattattatg tctccctcgg gaaaaatata tgtctaacac ttccaccatg ttgatgaact
    60301 tgtaaatgtg gtaaatcacc atcaggattt cctggtccat gacctgacac tctatatgga
    60361 ggttttgtta cagaaggatc ttttatatca tcccaatgat atgaacctgc ttttgtttta
    60421 tttccagtaa gatattccat taagttctta gtgctggggc ctgactcacc tgtcatattt
    60481 ttataattat gacctaaata tctatccata aacaattgtt cagcgtcact tctagaccta
    60541 acagtaactt tggtttcact actatctaat aaacgattcg ctttttggtt actaacagca
    60601 catggagtta atccaagtgg atcagcatat tttagaggat taggtgcata actgtagaga
    60661 tttaaaccgc ctctcaaccc tatcgggtca gttaccatga accgcccgac atccggatca
    60721 tagtacctgt gcagattgta gtgcagcccc gtttccctgt cgaggtactg tccctggtag
    60781 cgcaggttct gctccacccc gccgtggtcc ttctcctgta tcgtgttacc ccagacctga
    60841 taccccgtct cccacacgat tttcccgtcg ctgtccgtca tctcttccgg cgcgccgttc
    60901 acatccgtgt gaaaatacag tatccggttc gggccctctt tgcccgcctt atccacccgc
    60961 gccagcggtt cataactccc ctggtccctg tagatgtaca ggctgcctgt tccctccggc
    61021 ctgctctcct gcgccagccg cattccgtcc cataaaaatt ttgtccggtt gtacggctta
    61081 ccgtcagggc tgatgtgctg tttttccgtc cgcctcccca gtgcatcgta cacataccgg
    61141 tatgtccccc gcgctgtcgt cacttccacc atccggtgtt ccgcatcata acggtaactc
    61201 tgcgttccgc tccggccctc cttttccacc gcacggccaa attcgtcata acggtagtgc
    61261 acaccacggt aagacatcag ccggttgaac ggcaccacgc tgttttctgc cgtggccctt
    61321 tcccccgcgc gcctccccag caggtttccc gccgcgtcgt actgccagct cgccagatac
    61381 ccctcatcct ccgaacgaca cgccgtgatg ctccccgtgg ggtcgtagaa gtaatccgtt
    61441 tccccccgcc ggctgtgccg cgtcttcagc agttcatcct cgccactgta gctgtactgc
    61501 cggtccgtga acgtctccgg taccaccccg cgcatcccgc tgtatgtctc cctgcgcttc
    61561 agccgcccgc cggggtcgta aagctgccgc gtgctcagtc gcccctggct tctgagcact
    61621 tcacggtgca ggtggtcgcg cgtgaactcg ctgatgacct cccggtccag atttatctgc
    61681 tggagatggc cgctgccgta gtacagatgg cgctgcgttc tgccgtccgg aaaggtggtg
    61741 gcagtccggt tgcccagttc atcgtactgg tgggtcagga tgccctgcgc cgtctcctcg
    61801 ctcagcatcc gccccagtct gtcgtagctg aagcggataa cttccggcgc gtcagtttcg
    61861 tgccgctggc gacggatttc cagtagctgg tctgccgcat catggacgta ttccgtacgg
    61921 ccatcggctg tctcttttgc cgtgagccgg cccgccgcat cacgcgccag ccggtggcgg
    61981 atttcaccgc cccggtcccc ggtgaacacc acttccgtga ccgcgttcag cgcgttgtag
    62041 cggtacgact ggcggcagcc gtccgggttt atctgctccg ccagacggtc cagcacatca
    62101 tggcggaacc agtagcgttc tccgttctcg ttcgtgagcg tgaccagacg cccgtaagcg
    62161 tcgtacccga atgccgtcct gcgccccatc gcgtccgtcc ggctgcgcac ccggccctgc
    62221 ccgtcccgct gccagcgcgt tatcctcccc atcgggctgg tgtactttac cagcctgccc
    62281 gccgcgtctg actggtaatt caccgtgcgg ccgtccgcca gtatcacctc cgtcagatga
    62341 ccgctgtcgt catacccgta gcgcgtgctg tggctctgcg catccgtcat tctgataagc
    62401 ctcgtgcgtt cgtcgtagaa ccacgccgtc tgcgagccgg aacagtccgt gtgccgcatg
    62461 agctgcccgt cttcattcca ctggaggtat ttattccccc cgcgcgcatc tgttatctgc
    62521 accacctggc cgtggtggtc atactgatac cgggtgttct gctgcagcgg gtcggtcacg
    62581 gcaagaaggt tgccgcgttc atcgtacaca cagcgccagg tgtttcccgc cgcgtccacc
    62641 tccgtttccg gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg
    62701 tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga
    62761 gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt
    62821 ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg
    62881 cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac
    62941 tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca
    63001 taccagcccc actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag
    63061 tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc
    63121 aggtggccgg cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa
    63181 tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg
    63241 cacggacgct gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga
    63301 ttcagacagt tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc
    63361 gggtcagcct caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc
    63421 tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg
    63481 gcttccatat ccaggcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg
    63541 ctacccggtg tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt
    63601 ccttccgtac gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc
    63661 ggcagactga aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg
    63721 accggatgcg gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca
    63781 gcaaaacagg cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt
    63841 gccaccaggg ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg
    63901 ccaatgcgca catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc
    63961 tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc
    64021 gcgccttccg cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg
    64081 atgctgccaa taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc
    64141 agcaggttaa gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga
    64201 aactgcgtca gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg
    64261 gagcccgggg ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg
    64321 ccatccacca tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac
    64381 atcagcacag gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga
    64441 tattccccgg ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt
    64501 tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct
    64561 gcccgcgcgg caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct
    64621 tcaatctgag gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct
    64681 gtcacgccgg tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg
    64741 ccccccatcg tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca
    64801 tcaataacga catttgcagc cacctccagc accgccccca gcacatctgc cataaacgac
    64861 gtatgcagca gggcatcccc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc
    64921 ctgtatgatt aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt
    64981 tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc
    65041 atcatgaccc gctcacccgg caatacaaat gccacctgac actggtacac ctgctcgtta
    65101 ccccggagaa actggctctg tatttccaaa ccgcctcgtt gagataatat tccgtcataa
    65161 tgtaaatagt ccgttttaat aaaggaatta tttattgaca ggttctgttt ttttaatcat
    65221 gaatgtaata cccatcaccg ctttacagca cactttatct ttccagccat aatcactaat
    65281 aatattaagc aattagcaat atttacaacg accagagcgg atacatagtt atgtatttaa
    65341 caatcccaat atattcacta tgcaaataaa accactagta acagacacgc taccgcctcc
    65401 atataaaaat ggacacctta taaaattgta tcttgcatag aaaaagctga aatatcggat
    65461 gcgttttaat tatcaactat aaatctataa gctttttatt aacaaca
//
LOCUS       DAJTRQ010000010        60673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000010 DAJTRQ010000000
VERSION     DAJTRQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..320
                     /locus_tag="OO332_000733"
     CDS             <1..320
                     /locus_tag="OO332_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5941316.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="OO332_000734"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="OO332_000734"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5941317.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="OO332_000735"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="OO332_000735"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5941318.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="OO332_000736"
     CDS             complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="OO332_000736"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5941319.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="OO332_000737"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="OO332_000737"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5941320.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="OO332_000738"
     CDS             complement(3724..4953)
                     /locus_tag="OO332_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5941321.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5017..5457)
                     /locus_tag="OO332_000739"
     CDS             complement(5017..5457)
                     /locus_tag="OO332_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5941322.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="OO332_000740"
     CDS             complement(5562..6332)
                     /locus_tag="OO332_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5941323.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="OO332_000741"
     CDS             complement(6329..7255)
                     /locus_tag="OO332_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5941324.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="OO332_000742"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="OO332_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5941325.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="OO332_000743"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="OO332_000743"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5941326.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /gene="gcd"
                     /locus_tag="OO332_000744"
     CDS             8809..11199
                     /gene="gcd"
                     /locus_tag="OO332_000744"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5941327.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="OO332_000745"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="OO332_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="OO332_000746"
     CDS             12961..13308
                     /locus_tag="OO332_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5941328.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="OO332_000747"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="OO332_000747"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5941329.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="OO332_000748"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="OO332_000748"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5941330.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="OO332_000749"
     CDS             complement(15128..15490)
                     /locus_tag="OO332_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5941331.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="OO332_000750"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="OO332_000750"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5941332.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="OO332_000751"
     CDS             18618..20471
                     /locus_tag="OO332_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR5941333.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="OO332_000752"
     CDS             complement(20542..21972)
                     /gene="lpdA"
                     /locus_tag="OO332_000752"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5941334.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="OO332_000753"
     CDS             complement(22297..24189)
                     /gene="aceF"
                     /locus_tag="OO332_000753"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5941335.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="OO332_000754"
     CDS             complement(24204..26867)
                     /gene="aceE"
                     /locus_tag="OO332_000754"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5941336.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="OO332_000755"
     CDS             complement(27028..27792)
                     /gene="pdhR"
                     /locus_tag="OO332_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5941337.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28333..29706
                     /gene="aroP"
                     /locus_tag="OO332_000756"
     CDS             28333..29706
                     /gene="aroP"
                     /locus_tag="OO332_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5941338.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="OO332_000757"
     CDS             complement(29749..30603)
                     /gene="ampE"
                     /locus_tag="OO332_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5941339.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="OO332_000758"
     CDS             complement(30600..31151)
                     /gene="ampD"
                     /locus_tag="OO332_000758"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5941340.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31239..32132
                     /gene="nadC"
                     /locus_tag="OO332_000759"
     CDS             31239..32132
                     /gene="nadC"
                     /locus_tag="OO332_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5941341.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32335..32775
                     /gene="ppdD"
                     /locus_tag="OO332_000760"
     CDS             32335..32775
                     /gene="ppdD"
                     /locus_tag="OO332_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5941342.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32785..34170
                     /gene="gspE"
                     /locus_tag="OO332_000761"
     CDS             32785..34170
                     /gene="gspE"
                     /locus_tag="OO332_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR5941343.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34160..35362
                     /gene="hofC"
                     /locus_tag="OO332_000762"
     CDS             34160..35362
                     /gene="hofC"
                     /locus_tag="OO332_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5941344.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="OO332_000763"
     CDS             complement(35397..36440)
                     /gene="guaC"
                     /locus_tag="OO332_000763"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5941345.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36602..36640)
                     /locus_tag="OO332_000764"
     CDS             complement(36602..36640)
                     /locus_tag="OO332_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941346.1"
                     /translation="MIIKTLKMSARK"
     gene            36665..37285
                     /gene="coaE"
                     /locus_tag="OO332_000765"
     CDS             36665..37285
                     /gene="coaE"
                     /locus_tag="OO332_000765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5941347.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37285..38028
                     /gene="zapD"
                     /locus_tag="OO332_000766"
     CDS             37285..38028
                     /gene="zapD"
                     /locus_tag="OO332_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5941348.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38038..38235
                     /gene="yacG"
                     /locus_tag="OO332_000767"
     CDS             38038..38235
                     /gene="yacG"
                     /locus_tag="OO332_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5941349.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="OO332_000768"
     CDS             complement(38335..38724)
                     /gene="mutT"
                     /locus_tag="OO332_000768"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5941350.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38784..41489)
                     /gene="secA"
                     /locus_tag="OO332_000769"
     CDS             complement(38784..41489)
                     /gene="secA"
                     /locus_tag="OO332_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5941351.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41551..42063)
                     /gene="secM"
                     /locus_tag="OO332_000770"
     CDS             complement(41551..42063)
                     /gene="secM"
                     /locus_tag="OO332_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5941352.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="OO332_000771"
     CDS             complement(42294..43211)
                     /gene="lpxC"
                     /locus_tag="OO332_000771"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5941353.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="OO332_000772"
     CDS             complement(43312..44463)
                     /gene="ftsZ"
                     /locus_tag="OO332_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5941354.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="OO332_000773"
     CDS             complement(44524..45786)
                     /gene="ftsA"
                     /locus_tag="OO332_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5941355.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="OO332_000774"
     CDS             complement(45783..46613)
                     /gene="ftsQ"
                     /locus_tag="OO332_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5941356.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46615..47535)
                     /gene="ddlB"
                     /locus_tag="OO332_000775"
     CDS             complement(46615..47535)
                     /gene="ddlB"
                     /locus_tag="OO332_000775"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5941357.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47528..49003)
                     /gene="murC"
                     /locus_tag="OO332_000776"
     CDS             complement(47528..49003)
                     /gene="murC"
                     /locus_tag="OO332_000776"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5941358.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49057..50124)
                     /gene="murG"
                     /locus_tag="OO332_000777"
     CDS             complement(49057..50124)
                     /gene="murG"
                     /locus_tag="OO332_000777"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5941359.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="OO332_000778"
     CDS             complement(50121..51365)
                     /gene="ftsW"
                     /locus_tag="OO332_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5941360.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51365..52681)
                     /gene="murD"
                     /locus_tag="OO332_000779"
     CDS             complement(51365..52681)
                     /gene="murD"
                     /locus_tag="OO332_000779"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5941361.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52684..53766)
                     /gene="mraY"
                     /locus_tag="OO332_000780"
     CDS             complement(52684..53766)
                     /gene="mraY"
                     /locus_tag="OO332_000780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5941362.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53760..55118)
                     /gene="murF"
                     /locus_tag="OO332_000781"
     CDS             complement(53760..55118)
                     /gene="murF"
                     /locus_tag="OO332_000781"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5941363.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55115..56602)
                     /gene="murE"
                     /locus_tag="OO332_000782"
     CDS             complement(55115..56602)
                     /gene="murE"
                     /locus_tag="OO332_000782"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5941364.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56589..58355)
                     /gene="ftsI"
                     /locus_tag="OO332_000783"
     CDS             complement(56589..58355)
                     /gene="ftsI"
                     /locus_tag="OO332_000783"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5941365.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58371..58736)
                     /gene="ftsL"
                     /locus_tag="OO332_000784"
     CDS             complement(58371..58736)
                     /gene="ftsL"
                     /locus_tag="OO332_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5941366.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58733..59674)
                     /gene="rsmH"
                     /locus_tag="OO332_000785"
     CDS             complement(58733..59674)
                     /gene="rsmH"
                     /locus_tag="OO332_000785"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5941367.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59676..60134)
                     /gene="mraZ"
                     /locus_tag="OO332_000786"
     CDS             complement(59676..60134)
                     /gene="mraZ"
                     /locus_tag="OO332_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5941368.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14533 a        16800 c        14918 g        14422 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaatttg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc caggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctctacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgagacac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac gcctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg caaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc caaggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgagctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaacagtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcgacagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcggcaggtc
    20581 ggtaatgcta ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg
    20641 cgcgtggatg gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccagcccgat
    20701 ttcacccagc agctcgccgc cgttggtacc aacaatcgca ccaccgatca cgcggtgaga
    20761 ttctttgtcg aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc
    20821 agaagcagcc cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt
    20881 ctcagtcaga cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt
    20941 cggatcgaag tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac
    21001 acctttgtgt gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac
    21061 gttggtacgc agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc
    21121 tttgcctgcg tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc
    21181 gtaacgctgc ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc
    21241 tttcgcttca acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg
    21301 cttggtgaag actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc
    21361 aaccacgtca atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat
    21421 accgccaccc attaccagca ggcgttctgg cacttctttc agttccagcg cgtcagtgga
    21481 gtcccagata cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc
    21541 tgcaatgatc gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag
    21601 ggtgttagcc ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc
    21661 cataccagcc agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt
    21721 gtcaatatcg gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc
    21781 ttcgataact tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca
    21841 aacaccgcca agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca
    21901 acggaaggca gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat
    21961 ttcagtactc atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca
    22021 ggccgcagcg gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg
    22081 ggattctggc gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac
    22141 cctaaccacc accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta
    22201 aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta
    22261 ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc
    22321 agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg
    22381 gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca
    22441 caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat
    22501 cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg
    22561 catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg
    22621 agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt
    22681 cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc
    22741 ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat
    22801 gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg
    22861 ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg
    22921 gatcatcacc cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc
    22981 cacttcttcg atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg
    23041 gataccaccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta
    23101 agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag
    23161 gttaacgcca aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc
    23221 gttttcagca aattcagatt tgccttccgc tttcgcagcc ggagctgctg ccggagcttc
    23281 agcttttgct gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc
    23341 tgcgccttca acttcgaaga tcataatcag cgaaccagtt ttcactttat cgccaacgtt
    23401 gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc
    23461 gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac
    23521 ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc
    23581 tgctggtgcg ggggtcgctg ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc
    23641 tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac
    23701 gttcactttg atctctttca cggtacctgc aaacggagcc ggaacttcca tagaagcctt
    23761 gtcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag
    23821 gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc
    23881 cgcagccgcg ggtgctgcta ccggagctgc ttctttcttc tcttctgcct gagcaggtgc
    23941 agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt
    24001 atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagatg gaacttccat
    24061 agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac
    24121 tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc
    24181 gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg
    24241 ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt
    24301 tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc
    24361 tcacggctgt cggaacgacc gaagccatca gtacccagta cgcgatagtc gtcagccggt
    24421 acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccggc
    24481 gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg
    24541 ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt cacgctataa
    24601 acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc
    24661 aggatggaac cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt
    24721 ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag
    24781 ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc
    24841 tccagaccgt catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata
    24901 cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca
    24961 ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtct
    25021 ccagccgccc agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag
    25081 aatgggatca tcggcagatt gttcgtgctg taagaggtcg ccgctgccag ccaggaacac
    25141 cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag
    25201 taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca
    25261 atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc
    25321 agacgatctt tgatcgactt gttcttcagc atcacgttca gagcacgaac gaaagcgata
    25381 gtggtagaga gctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc
    25441 agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga
    25501 cgctgagcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt
    25561 ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg
    25621 tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg
    25681 ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt ttcctgcgct
    25741 ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc
    25801 tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt
    25861 tcacgaacgt acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg
    25921 ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta
    25981 ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg
    26041 atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg
    26101 aaaaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt
    26161 tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg
    26221 ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga
    26281 cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa
    26341 gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga
    26401 ccttccagga aagcacgggc gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg
    26461 tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg
    26521 gttgcggaag actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca
    26581 cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc
    26641 agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata
    26701 cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc
    26761 agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag
    26821 tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta
    26881 tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc cagaaagctc
    26941 aataagacag gttctacgtt tagttaccgc gcttttatat gcgcttgatt tacaacatct
    27001 tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga
    27061 acgctcacgg cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag
    27121 atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat
    27181 gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa
    27241 gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac
    27301 cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc
    27361 gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac ggatgcgttc
    27421 cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg
    27481 tcgtgtttcg agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg
    27541 atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag
    27601 caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc
    27661 gaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc
    27721 gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt
    27781 gctgtaggcc atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt
    27841 tcatgtctgt gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg
    27901 aaatcatgaa actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac
    27961 tatgcacaaa tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa
    28021 aatccgaatt gaaccgattc acttaccaat tttttgattt ttaagtcaat aaaaacgaat
    28081 ttaaattcac tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt
    28141 aagcaactca tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc
    28201 ttgatctgac ggaagtcttt ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata
    28261 caacaaacgg aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc
    28321 gcacgaggtt tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa
    28381 aaccgccata ttcagcttat cgcgctgggt ggcgcgatag ggacagggct attcctgggt
    28441 agcgcctccg taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt
    28501 tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca
    28561 ggctccttta gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc
    28621 tggaactact gggtactgta cgttttagtt gccatggcag agctgactgc cgtgggtaaa
    28681 tacattcagt tctggtatcc ggaaatccca acctgggttt ctgccgccgt attctttgtg
    28741 gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga gttctggttt
    28801 gccattatca aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc
    28861 agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg
    28921 ccgcacggct tcaccggact ggtgatgatg atggcgatta tcatgttctc gttcggtggt
    28981 ctggaactgg tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa
    29041 gcaactaacc aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg
    29101 ctctcactga tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc
    29161 cacgagttag gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg
    29221 ctctccgtgt acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa
    29281 caggggaacg cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc
    29341 attctggtgt ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa
    29401 tccgcatttg gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg
    29461 atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc
    29521 ttccctgctc tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta
    29581 ctggtgatta tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg
    29641 ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg
    29701 cattaatctc tctacgccct cacccgtaca gggtgagggc aataatctct acaccaacgc
    29761 cccgtaaatc gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat
    29821 tgaaaccgct gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa
    29881 ctgcgccaga cgcgttaata cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa
    29941 ccaggccggt aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg
    30001 aaccggcacc caatccagta catgaagcac tgcatcaatg ccagactgta aacgatgatg
    30061 cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc aagaacgcat accccatcag
    30121 cgtaacgggt ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg
    30181 aaagttaatc cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc
    30241 tgccgggacg ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc
    30301 attacgtgaa gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat
    30361 acacagcaaa ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg
    30421 caatgcgcgt aacagtaaaa aagtcacacc catcgcaata atggtcatgc ctaacgtgcg
    30481 cccgagagaa aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca
    30541 gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt
    30601 catgttgtct ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga
    30661 tcggttttcc gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatcggga
    30721 tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac
    30781 gccagagtat cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcacgc
    30841 ccctgatact gagagactcc cgcatgccag gcacgtttat cgaaaggaac atactggact
    30901 atttcaccat cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca
    30961 aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg
    31021 ccaaactcgc ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca
    31081 tccaggcggc aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt
    31141 tctaacaaca tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt
    31201 ttctacctta tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctacaacc
    31261 ctgacacccg acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg
    31321 cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa
    31381 aacttttacc ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat
    31441 tttgtggcaa acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca
    31501 taatctggca tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg
    31561 aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc
    31621 tttcaggagt tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc
    31681 agttgttgga tacgcgcaaa accttacctg gcctgcgttc agctctgaaa tacgcggtac
    31741 tttgcggcgg cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa
    31801 accatattat tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc
    31861 cggatgcgcc agtagaagtc gaagtagaga atctggaaga acttgatgag gccctgaaag
    31921 ccggagccga tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca
    31981 aacgcaccaa cgacaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc
    32041 gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac
    32101 aagcactcga cctttcaatg cgttttcgct aattgctgac agatttatcc gctccatcgc
    32161 gggcggataa tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt
    32221 cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg tttcgttttt tgcccctcgc
    32281 ctgtgctgat ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac
    32341 aagcaacgcg gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta
    32401 agcgccattg gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg
    32461 ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga
    32521 ttagatacct gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt
    32581 tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg
    32641 ctaagcgtcg tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc
    32701 aactgcaata ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt
    32761 gatgacgcca actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt
    32821 tatcagggag tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc
    32881 ccctcacatg agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc
    32941 tgctggacgc gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta
    33001 gctgttcagg agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg
    33061 ctggaacaac gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc
    33121 ttgcgtatcg acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca
    33181 ttaacagcca gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag
    33241 gacgggcaat tcactgtcga actggcagga aacaccgtct catttcgtat tgcgacctta
    33301 ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg
    33361 gatgttaaca cgctgggaat gcagccgtta caactggcgg actttgctca tgccttgcaa
    33421 caaccacagg gactggtgct ggtaactggc cctacaggca gcggcaaaac ggtcacgctt
    33481 tatagtgccc tgcaaacgct gaataccgct gacattaata tttgtagcgt cgaagatccg
    33541 gttgagatcc ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cgggctcacc
    33601 tttcagggtg ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag
    33661 atccgcgatg gcgaaacggc agaaattgcc attaaagccg tgcaaaccgg tcacctggtg
    33721 ttgtccacgc tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg
    33781 gttgcccgct ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt
    33841 aaactttgcc cacattgtcg ccgacagcaa ggggagccca tccatattcc agacactgta
    33901 tggccgtcgc cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt
    33961 tatggtcgta ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt
    34021 tccgctaata ctgaagttga atcgctggaa acgcacgccc gacaggcggg tatgcgtacg
    34081 ctttttgaaa acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc
    34141 cgcgtactgg ggatgccgca tggcaagtaa gcaactctgg cgctggcatg gcataaccgg
    34201 cgacggcaat gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc
    34261 actacagcaa cagatggtta ccccactaag cctgaagcga atcgccatca attctgcgca
    34321 gtggcgagga gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg
    34381 gttaacgctt tctgaagggc tggctctgct ggcggaacag catcccagta aacaatggca
    34441 agcgttgctg caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt
    34501 attaccctgg tcagaggcat ttccgccgct ctatcaggcg atgatccgca cgggtgaact
    34561 gaccggtaag ctggatgaat gctgctttga actggcgcgt cagcaaaaat cccagcgtca
    34621 gttgaccgac aaagtgaaat cagcgttgcg ttatcccatc atcattttag cgatggcaat
    34681 catggtggtt gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac
    34741 cttcaacacc ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg
    34801 cgaatggggc tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct
    34861 gatgcgccgc ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat
    34921 gggttcactg atgaggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca
    34981 aagtgcaggc attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc
    35041 gtactgggtg caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg
    35101 gctggcgcta aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacagg
    35161 agaggcatcc ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa
    35221 cacaatggcg ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac
    35281 gggaggaatt atcggtacgc tggtggtggc aatgtatctg ccaattttcc atttaggcga
    35341 tgcgatgagt gggatgggat aacgctggcg tgttgctcca cgccagcgtt gggagattac
    35401 aggttgttga agatgcggtt ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc
    35461 agctctttca ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata
    35521 tcacgcgcgg tattttcaac cgggcctcgc agcggcagct tgacggtttt accttctgct
    35581 gcgcggtatt ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg
    35641 tagaacagca taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg
    35701 cccgccagca tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca
    35761 tcgcccggcg tggtacagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca
    35821 tcggcacatt cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt
    35881 gtacaaacag aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc
    35941 tcctcacaca tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct
    36001 tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata
    36061 caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca
    36121 tcagacgtgc cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata
    36181 aacgcttgcc actcttcgac agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa
    36241 gccagcgcag aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc
    36301 acgccggacc agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg
    36361 gaacggcttt taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc
    36421 agatcttctt caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg
    36481 caactccagt gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct
    36541 ttttttacgc tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat
    36601 ttcactttcg cgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata
    36661 aattatgggg tatatagttg ccttaacggg aggcattggc agtggtaaga gtaccgttgc
    36721 caatgcgttt gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt
    36781 ggttgaacca ggtgcacctg cgctacatgc cattgctgat cactttggcg aaaacatgat
    36841 tgctgctgat ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga
    36901 agagaaaaac tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca
    36961 gatccagcaa gcaacttccc cctatgtact gtgggttgtg ccattgctga tagaaaactc
    37021 actgtataaa aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa
    37081 gcgcaccatg cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca
    37141 ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc
    37201 ggatgctatc gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca
    37261 gtttgtctca caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat
    37321 gaaaaaatgc gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta
    37381 cccatcgttg accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat
    37441 gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt
    37501 aaactccaga cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt
    37561 cagcagttaa aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg
    37621 cgtgaagatc gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc
    37681 agctttgatt tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc
    37741 caggtagaaa cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg
    37801 gatttaattc gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag
    37861 gataacggtg gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat
    37921 ccgcaaattt ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa
    37981 aacggacagg taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg
    38041 tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc
    38101 agcccgtttc ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct
    38161 gctgaagaaa aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa
    38221 gagccaaagc agtgacattt gtctgatgcc tcacgtaggc ctgataagac gcggacagcg
    38281 tcgcatcagg catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag
    38341 acgcttaagc ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc
    38401 gaccagcgac atccactcac cgggttgccc ttctttaccc cacggcaccc cttcccaact
    38461 ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc
    38521 aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac gcaccaccgc
    38581 ctgttccggc gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat
    38641 gtgcgcatct gctgcgcgac gtgttataaa gatttcattg ttctcgttgc gaataatacc
    38701 taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg
    38761 ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta
    38821 ccagaaccgc acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc
    38881 agtgcagctg cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa
    38941 cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc
    39001 tgaactttgc tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac
    39061 atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg
    39121 tggataccct gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc
    39181 gtttgcagca tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct
    39241 ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc
    39301 agttctggtt ctttatccag ccactcggca attggtaaat cgaggtcgaa atcgttcttc
    39361 agacgttcct gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag
    39421 gcatcaatgg tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg
    39481 ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg
    39541 tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg
    39601 gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctag cttcataccc
    39661 agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc
    39721 atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac
    39781 tggttatcga tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct
    39841 tccagtaccg catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg
    39901 gtcggatttt ccagcgcggc aacttctgcc tgccagctac ctccgagcac aatatctgta
    39961 ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca
    40021 atcgccgctt cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg
    40081 gtcagttcgt ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc
    40141 tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca
    40201 gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg
    40261 acggtatcca gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc
    40321 atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt
    40381 tggttttcgt tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg
    40441 gaccagcgtc ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca
    40501 tctttaacga tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt
    40561 acgtggtgca tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg
    40621 ccctctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg
    40681 cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt
    40741 tcctgacgga tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct
    40801 tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact
    40861 tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccatg
    40921 ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg
    40981 taagcttcgc gctttgccgg tgctggcatg cccggcaggt tgataccaac agtcaggcca
    41041 aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg
    41101 gtaactacgt gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc
    41161 agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg
    41221 ccgagtaact gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc
    41281 acggcgaaag cttccgggat cagattttcc agcacttcgc ctttttccag acgcgcacga
    41341 aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc cggttccatg
    41401 gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg
    41461 aaaactttag ttaacaattt gattagcata ataaaatctc aaacgccccg cgttacggag
    41521 ttaataaaat gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat
    41581 gccttgcgcc tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc
    41641 ataatcaatg cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag
    41701 cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag
    41761 tgtttgcggt gccattgcga aagaaagatg acggattacc gtgcgaatag catgttgatg
    41821 ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa
    41881 ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc
    41941 gtttggttcg gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa
    42001 taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact
    42061 cacgttattg ccatcccgat cgaattcaaa cgtatttagc gcacaaatct tatcattaaa
    42121 gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta agaaaacagc
    42181 gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc
    42241 cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc
    42301 agtacagctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca
    42361 tattcccagg cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga
    42421 ccggatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg
    42481 atcgcatcga gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc
    42541 agtacgcgat aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca
    42601 cgggactgca gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg
    42661 cgcataaacg catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc
    42721 ggatggttaa aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc
    42781 cacttatcgc catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag
    42841 ttcaactcgt cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc
    42901 atgatcggga tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg
    42961 agagcagcat tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac
    43021 gtacagagca tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc
    43081 aagtcggtgc gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg
    43141 actttcttgc cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt
    43201 tgtttgatca tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc
    43261 ggcgggccag tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc
    43321 ttgcttacgt aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc
    43381 attgtcattc actactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg
    43441 ctgctggtag cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc
    43501 aggacgtttg tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat
    43561 atccgggtca agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat
    43621 ggtgttacct accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac
    43681 cagcacgccg cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat
    43741 ttcagcagct tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta
    43801 gcccatttca gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg
    43861 agtaatcagt tcggcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa
    43921 cgcatccagc agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt
    43981 gatcagagag tccacatgct tggacagttc agtgatcccc tgctcggcga atgccatacg
    44041 cttcttgcct tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc
    44101 ttttgccact tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc
    44161 agcaataaag accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc
    44221 agccgcattg cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct
    44281 accgatttga atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac
    44341 cgcgaagaat tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc
    44401 gccgccgccg acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa
    44461 catagtttct ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa
    44521 aaattaaaac tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc
    44581 tgtaacacgt ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag
    44641 caatcccacc gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt
    44701 cagcggcgcg ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc
    44761 ttcgatctgc gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc
    44821 ttgttggcga agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc
    44881 ggtatagcgc ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg
    44941 accgcctacg ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca
    45001 accgtggcga actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat
    45061 atcactggtc acgacattgc cagcataagg aattacctta gtgtggcgta atgccccacc
    45121 ggtataaacg gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc
    45181 acgttcatct tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc
    45241 aactttcagc ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca
    45301 tgtgatcagg tgcacttttg cctgcatccg cacacccgaa agtcctaccg gattcttgat
    45361 cccttcctga tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc
    45421 gcgcacacgc accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc
    45481 ttcttcagaa ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag
    45541 cgccagatat accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg
    45601 ttgtacgcac ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga
    45661 cgggcagctg cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa
    45721 agcggcaacc ttcgcggtac caatttccag tcctactacc agttttctgt ccgtcgcctt
    45781 gatcattgtt gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag
    45841 ggcgcccagc ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg
    45901 ccatcggttt gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt
    45961 ttcatcgtat cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag
    46021 gaacgccgcg cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc
    46081 cccatttcgc gatagccctg caacacttca ttagcgctgc cttccgggcc atacagcatt
    46141 ggaagcacct gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct
    46201 accatgtgtt gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat
    46261 tcatcaggcc actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt
    46321 tgcgtctgga tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg
    46381 atcgactgcc ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt
    46441 gagagcggca ggcgttgcgc atcttccatc cagctcaaca cgacccagcc gctcaccaac
    46501 actgtcgtta aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg
    46561 cgagaagaaa cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc
    46621 cgccagttcc agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc
    46681 catcggcacc aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa
    46741 ctgtccatca ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt
    46801 ccatgctttc agcactaatg cctgcaaatt ggcctcttgt gacgctttca gacctgcggg
    46861 gcagaaatac tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga
    46921 cggttgaata cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg
    46981 cccacttagc catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc
    47041 atcttgtaga gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct
    47101 cggcttaacg ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag
    47161 gcctttttca aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc
    47221 ttgccacaga agtttgctgc gtagtttatc tattgaaagc gcagatgcca tcactccgct
    47281 gccggtataa ggcaagccca tcagctcgag catgccctgc agcgtaccat cttcaccgcc
    47341 gcgaccgtgt agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc
    47401 gacttctttc gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac
    47461 cgctgcgcca gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc
    47521 gattttatca gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta
    47581 aagaacgggc aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg
    47641 ttaataccgg tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa
    47701 ttttcccacg tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg
    47761 cttcgccagc cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg
    47821 cgaaatcatc atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacca
    47881 ggtttttatc cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt
    47941 ggtggccgta gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg
    48001 ggaattcacc gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca
    48061 aaatagcctc gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca
    48121 gcgcgttatg acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctgacgca
    48181 gcagcgtaaa gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat
    48241 cttcgctgaa gccgtaagtt gtggtctgac gtcccacacg cggtaacaat tcgcggatca
    48301 ccggatcatc aacacacatc accgcacgac cgtaaaaagg caggttgtgc agaaaattaa
    48361 taaaggtctg ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga
    48421 tattggtgac aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg
    48481 cttcggcaat caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca
    48541 gcccgccgtt aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg
    48601 cggtagttgt cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta
    48661 actcagccag catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa
    48721 tttccgggtt gtcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt
    48781 tttccgggcg atggttgaaa taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg
    48841 gatttggcgc taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa
    48901 taccgcccat accggcacca ccaatgccga caaaatgtat gtgccgaacg cgacgcattt
    48961 cgggcacgat ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact
    49021 tcttaaattc atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac
    49081 ttcatttgcc actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat
    49141 ggttaataag gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag
    49201 ctgtggctgc tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta
    49261 ttgctggcgg tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat
    49321 ttcactcaca gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc
    49381 cgccatatca tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc
    49441 ctgttcaacg gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc
    49501 cagtttcgca gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc
    49561 cactaccaac acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag
    49621 cgccagcaca tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg
    49681 aaacgcctgc atcactttgg tagcaatctt cgccagccat ttattggtta agcccgcaat
    49741 accgttttgt tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg
    49801 acctgataca tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc
    49861 ccgcgcctga cgccagccgt tgaagatacg cagcggcgca gctatcagtg cttttatacc
    49921 ttttccacgc aaaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa
    49981 gtccgcttcc atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag
    50041 atagtgcgca accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat
    50101 taatcgcttt ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga
    50161 cgcgtttcat aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc
    50221 gaaccaccgt aactgattag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc
    50281 gcgcctacgt taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa
    50341 ccggaaaaac ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg
    50401 acgaagaata ccattaaaag tgctagtacc acaccgacat aacctaattc ttcgccgata
    50461 atggcgaaaa taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgaatta
    50521 cctaaacctt gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga
    50581 tagccgctgc caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg
    50641 tacggttcgg cgaggatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg
    50701 aactgccaca atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc
    50761 accaccgtac caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc
    50821 atcggtttca ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag
    50881 ttggcgatat agcaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa
    50941 ccgagatcga tccaacgcga tgccccttta acagcgctac ctactaccag cacgatcatc
    51001 agcaggatga tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc
    51061 agacgcagcg taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc
    51121 gcgaagaaga acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc
    51181 atgataaagc caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg
    51241 atcaggctgt cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag
    51301 atccagacca agatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa
    51361 cgcatcaacc taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct
    51421 tgaactgatc aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac
    51481 gcggagccag caagcgcata gcctgttcca tagtttcggt ttgttctgcc acttccgggc
    51541 gtagcgccgc cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat
    51601 tcaggtaacg cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat
    51661 gcagcgtgcc gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg
    51721 cttttgaatc gttaatccag cgcacaccgt tatgctccag caccacttca aagcgatgcg
    51781 gcagaccggt gaaggtggtt aacgctttca ggctgctggc gcgcggtaac cctgcggcat
    51841 ctgccagcgc cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct
    51901 ctttcacatt cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat
    51961 tcaggtgata gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa
    52021 tcggcattgt taaggcatca tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc
    52081 gcagttttgc tgcacgatac tgttgtaaac caaacgggta gcgatccata tgatcttcag
    52141 tcacgttcag aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc
    52201 tcgacagttc cagcacgtac agttcacact catcatccag tagcatcaac gcaggcaggc
    52261 caatattgcc acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg
    52321 tggtgaccgt gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc
    52381 gacaaaacag ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg
    52441 atggatgcgc cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcaaccatt
    52501 catcattcag actgcccgtg tggcgttcta cggcttcggg taatttatcc aggctaggcg
    52561 gtgtcatacg cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc
    52621 aggaaagccc ggtgaggccc aggccgataa tgacaacatt tttaccctgg taatcagcca
    52681 tgattaacgt accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca
    52741 gaaacgcaca atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg
    52801 tgccatgcgg aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga
    52861 aagcgtttct accacgaaca cgccccccat aatcaccagc aggaattcct gacgtagcag
    52921 tacggcgata atgcctaacg caccacctaa cgccagcgaa ccgacatcgc ccataaagac
    52981 ctgcgccgga taggtgttaa accacaggaa gcccagtcct gccccgacta tcgcggtaca
    53041 gacaataacc agttccccgg cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt
    53101 catgttgccg gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat
    53161 aattgccaga ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac
    53221 gaagtaagcc agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg
    53281 gaccaccagc tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc
    53341 cagcgcaatg accgacatcc agaaatattt ccaacgagcg atcaaccctt tggtatcttt
    53401 acgcaccact ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac
    53461 caacacgcac cagacgtacg gattggacgg gtaagcccac agcagcacgg agatcacaat
    53521 cgctgtcagg atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc
    53581 aggaccgtcg ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg
    53641 cgggcccatc cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt
    53701 cagataggaa aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac
    53761 taacatgtcc cattctcctg taaagcgcgt actacctctt ccatggcagc actacgtgaa
    53821 cccttaacta aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt
    53881 aacgcagttt tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt
    53941 tgtttaccca tgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt
    54001 acatggcagt cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg
    54061 tagcccggca tttcagccag tacctgaaca gctgcggtca ttgaaccgac attggcgttg
    54121 taggagtcgt cgagcagcaa ctggttttct gccagttgga tggggaacag acggccagga
    54181 acagctttca gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg
    54241 gagagcgcag cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga
    54301 acatcgacgc taccggttgg ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg
    54361 atattggtgg cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt
    54421 gagccaatta cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata
    54481 ccgttttccg gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag
    54541 ccaaaacctt ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc
    54601 agactcacag tcctggcaat ctcgccctga tggttcgcgc caagttcaat aactgcgtaa
    54661 tcgtattccg gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg
    54721 cctgccgtat aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg
    54781 gaggttttgc cggaggaccc cgtcagagca accacgcgcg ccggaacttg ctggcgaacc
    54841 catgcagcca gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg
    54901 atgtccagcg gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca
    54961 aaatcgtggg catcaaaacg ttcacctttc agggcaacaa acaggcagcc cggcgtcagt
    55021 tttcgggtat cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccgttg
    55081 agaatatcgg taagttggct aagggttacg ctaatcatgc aatccccccc agcagacgcg
    55141 ccaccgtgac gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcctcat
    55201 ggcctttgcc cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca
    55261 ccgcttcagc acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa
    55321 tatcgttgat gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca
    55381 cgtcagcaaa ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc
    55441 caccacagcc aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg
    55501 ctttttccag tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg
    55561 cagtgaacac ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat
    55621 cagccagtgg atagcccagc gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt
    55681 taaaagcgcc catcagatgg ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa
    55741 tggtcgcgcc gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt
    55801 tcggattgat atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc
    55861 ggcggcccac ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa
    55921 gcagccattt cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc
    55981 ttaagttggt aaagaccgac gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc
    56041 cgtgggagga aacttccatt gcgcaaaaag tcgcgccctg atccaccagc cccgccagct
    56101 catgctgaac atcaactgcc gaaccggtag tattttctgt cgggatcact ttccccagca
    56161 gaccgttacc aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg
    56221 ccagaagctg ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat
    56281 tgtcagaggg ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc
    56341 tgagatagat gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcatctt
    56401 tcgcctctgc aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc
    56461 cgtccgcctg atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt
    56521 cgagtgtcat ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt
    56581 cgcgcaaatt acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta
    56641 tcgcccgttg tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc gcccatgatg
    56701 gcaccaaaga ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg
    56761 ataacaacaa ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc ggtataagca
    56821 atatatttat tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg
    56881 gcgatacgat agcctttaat cgccgccttc acgccgccgc cgcctggtag cgccacgctt
    56941 tccatcatat gcaccacggt gcgaacaatg gattccggga agacacgttc accgggaacc
    57001 ggggggtcaa ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg
    57061 tagactcgcg ctaactgtaa cggtgttacc attagcccgt agccgaaaga gaaggtggcc
    57121 ctctctatgt cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac
    57181 cccaaattgg tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac
    57241 ggcatcgcta acgccagctt ggaaacaccg acgttactcg acttctgtaa taccccggtc
    57301 agggttaatt cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga
    57361 atggtattga gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc
    57421 atcggtttaa ccgttgagcc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc
    57481 tctttcggcg tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc
    57541 acttcacctg tgttgacatc caccagcacg gcgctaccag attcagcctt gttaaaggcc
    57601 accgcattgt tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc
    57661 aggttgtgcg ctgcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg
    57721 tctttacgca caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca
    57781 acgccctcaa tcccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg
    57841 ccggacggat agtaacggcg agactcttca cgcagatgaa tccccggcag tttcagtttt
    57901 ttgatgtagt ccgccatgtc agagtttacc tgacgcgcca gataaataaa gcgccctttc
    57961 gggttggcgt taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc
    58021 gccttccagc ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc
    58081 caaatcgctt ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg
    58141 ccgcgggagg tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc
    58201 atatccgggg agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg
    58261 agaatacagc cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga
    58321 cgttttggtt tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc
    58381 actacgatat tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc
    58441 ctttccaccc ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca
    58501 atgtctaaag catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta
    58561 tggtgcgccg tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt
    58621 ggcagcttcc caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg
    58681 tggcttccca tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt
    58741 tcgtcctctc tgcaatacgc agaactgaac tacgggcacg agggttctca gccacctctt
    58801 cttcgcccgg cattaacttg cctagtgctc gcagctgacg gccacccagt tttttgagct
    58861 gctcttcagt catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa
    58921 aacgtttcac aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg
    58981 gggccagcac gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta
    59041 cccaaatgcg caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg
    59101 gcgttgcagc agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt
    59161 tacgctcgac aatggcgcgg gcaatgcgtt tggcaaaacg ctcttcacca taggttttca
    59221 atacccaggc gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac
    59281 gggttgggtc catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag
    59341 catcatcaag ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg atcttgccaa
    59401 taagatcgcg ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg atgatggaga
    59461 agcgcggatc atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca
    59521 gcaaacgccc ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa
    59581 aagtcccatc aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca
    59641 gcagcaccgt agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc
    59701 tccgataagt ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc
    59761 caggttgttt catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt
    59821 gtcagcccgg catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc
    59881 tgacattcgc tggcatgacc taacagtagg cgctgcacac ggcgctcaac cgggttcatg
    59941 ctcgacagac gcgataattt ttgctcgata atttcccatt caggcagggg gtaaagcagc
    60001 aggcacgggt gatgaatgtc aatggtgcaa accatttgac cggcagcgtt ctcaagcagc
    60061 tgttcccgat aacgggtagg cactgataag cgccctttgc tgtcgagatt gactaacgtt
    60121 gctccccgga acatgccagc ctcacccctt attcccactt tgccccacaa tttcccactg
    60181 aaaggagttt acggagcgga ggaaaagctt gtcaagccag tcacaaggca taccgaggta
    60241 aaaaacccga tgtttacggc taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc
    60301 aaattaacgt tatgaatagc tgtaagaaaa aatccctcat cgtcatcact gttttaaaat
    60361 gactcactat tttccacagc aaatataaag tgtcagtttg cgacgcgagc ggcattttag
    60421 gacatatctt ccccggttaa cagtccttgt tacgtctgtg tggcgcttga tgccagcggg
    60481 tttgcgcaaa agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa
    60541 aataatgcaa aaacgcgctg tcgatatcac ttaattattg tgaatttcag ctggtgagtt
    60601 aattaacggg cgggtttaaa accatttatc aataatagtc aggaatagtc ttatttactt
    60661 taaggatatt gat
//
LOCUS       DAJTRQ010000011        57868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000011 DAJTRQ010000000
VERSION     DAJTRQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..141)
                     /locus_tag="OO332_000787"
     CDS             complement(<1..141)
                     /locus_tag="OO332_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5941369.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTS"
     gene            296..868
                     /gene="yeiP"
                     /locus_tag="OO332_000788"
     CDS             296..868
                     /gene="yeiP"
                     /locus_tag="OO332_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5941370.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1091..2557
                     /locus_tag="OO332_000789"
     CDS             1091..2557
                     /locus_tag="OO332_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5941371.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2675..3661
                     /gene="yeiR"
                     /locus_tag="OO332_000790"
     CDS             2675..3661
                     /gene="yeiR"
                     /locus_tag="OO332_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5941372.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3700..4413
                     /gene="lpxT"
                     /locus_tag="OO332_000791"
     CDS             3700..4413
                     /gene="lpxT"
                     /locus_tag="OO332_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5941373.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4825..5391
                     /gene="mepS"
                     /locus_tag="OO332_000792"
     CDS             4825..5391
                     /gene="mepS"
                     /locus_tag="OO332_000792"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5941374.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5572..7128
                     /locus_tag="OO332_000793"
     CDS             5572..7128
                     /locus_tag="OO332_000793"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5941375.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7210..9024
                     /locus_tag="OO332_000794"
     CDS             7210..9024
                     /locus_tag="OO332_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5941376.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9025..10119
                     /locus_tag="OO332_000795"
     CDS             9025..10119
                     /locus_tag="OO332_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5941377.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10119..11144
                     /locus_tag="OO332_000796"
     CDS             10119..11144
                     /locus_tag="OO332_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5941378.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11146..12735
                     /gene="yejF"
                     /locus_tag="OO332_000797"
     CDS             11146..12735
                     /gene="yejF"
                     /locus_tag="OO332_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5941379.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12739..13083)
                     /locus_tag="OO332_000798"
     CDS             complement(12739..13083)
                     /locus_tag="OO332_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941380.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13416..14606)
                     /gene="bcr"
                     /locus_tag="OO332_000799"
     CDS             complement(13416..14606)
                     /gene="bcr"
                     /locus_tag="OO332_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5941381.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14634..15329)
                     /gene="rsuA"
                     /locus_tag="OO332_000800"
     CDS             complement(14634..15329)
                     /gene="rsuA"
                     /locus_tag="OO332_000800"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5941382.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15479..17239
                     /locus_tag="OO332_000801"
     CDS             15479..17239
                     /locus_tag="OO332_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5941383.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17364..17648
                     /gene="rplY"
                     /locus_tag="OO332_000802"
     CDS             17364..17648
                     /gene="rplY"
                     /locus_tag="OO332_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5941384.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17787..18794)
                     /gene="yejK"
                     /locus_tag="OO332_000803"
     CDS             complement(17787..18794)
                     /gene="yejK"
                     /locus_tag="OO332_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5941385.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18976..19203
                     /locus_tag="OO332_000804"
     CDS             18976..19203
                     /locus_tag="OO332_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5941386.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19223..20983
                     /gene="pbgA"
                     /locus_tag="OO332_000805"
     CDS             19223..20983
                     /gene="pbgA"
                     /locus_tag="OO332_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5941387.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21058..21134
                     /locus_tag="OO332_000806"
     tRNA            21058..21134
                     /locus_tag="OO332_000806"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21092..21094,aa:Pro,seq:ggg)
     gene            complement(21237..23828)
                     /gene="yejO"
                     /locus_tag="OO332_000807"
     CDS             complement(21237..23828)
                     /gene="yejO"
                     /locus_tag="OO332_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5941388.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24148..24795
                     /gene="narP"
                     /locus_tag="OO332_000808"
     CDS             24148..24795
                     /gene="narP"
                     /locus_tag="OO332_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5941389.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25006..26058)
                     /gene="ccmH"
                     /locus_tag="OO332_000809"
     CDS             complement(25006..26058)
                     /gene="ccmH"
                     /locus_tag="OO332_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5941390.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26055..26612)
                     /gene="dsbE"
                     /locus_tag="OO332_000810"
     CDS             complement(26055..26612)
                     /gene="dsbE"
                     /locus_tag="OO332_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5941391.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26609..28552)
                     /gene="ccmF"
                     /locus_tag="OO332_000811"
     CDS             complement(26609..28552)
                     /gene="ccmF"
                     /locus_tag="OO332_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5941392.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28549..29028)
                     /gene="ccmE"
                     /locus_tag="OO332_000812"
     CDS             complement(28549..29028)
                     /gene="ccmE"
                     /locus_tag="OO332_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5941393.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29025..29234)
                     /gene="ccmD"
                     /locus_tag="OO332_000813"
     CDS             complement(29025..29234)
                     /gene="ccmD"
                     /locus_tag="OO332_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5941394.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29231..29968)
                     /gene="ccmC"
                     /locus_tag="OO332_000814"
     CDS             complement(29231..29968)
                     /gene="ccmC"
                     /locus_tag="OO332_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5941395.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30010..30672)
                     /gene="ccmB"
                     /locus_tag="OO332_000815"
     CDS             complement(30010..30672)
                     /gene="ccmB"
                     /locus_tag="OO332_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5941396.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30669..31286)
                     /gene="ccmA"
                     /locus_tag="OO332_000816"
     CDS             complement(30669..31286)
                     /gene="ccmA"
                     /locus_tag="OO332_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5941397.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31305..31907)
                     /gene="napC"
                     /locus_tag="OO332_000817"
     CDS             complement(31305..31907)
                     /gene="napC"
                     /locus_tag="OO332_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5941398.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31917..32366)
                     /gene="napB"
                     /locus_tag="OO332_000818"
     CDS             complement(31917..32366)
                     /gene="napB"
                     /locus_tag="OO332_000818"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5941399.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32363..33226)
                     /gene="napH"
                     /locus_tag="OO332_000819"
     CDS             complement(32363..33226)
                     /gene="napH"
                     /locus_tag="OO332_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5941400.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33213..33908)
                     /gene="napG"
                     /locus_tag="OO332_000820"
     CDS             complement(33213..33908)
                     /gene="napG"
                     /locus_tag="OO332_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5941401.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33915..36401)
                     /gene="napA"
                     /locus_tag="OO332_000821"
     CDS             complement(33915..36401)
                     /gene="napA"
                     /locus_tag="OO332_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5941402.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36398..36661)
                     /gene="napD"
                     /locus_tag="OO332_000822"
     CDS             complement(36398..36661)
                     /gene="napD"
                     /locus_tag="OO332_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5941403.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36651..37145)
                     /gene="napF"
                     /locus_tag="OO332_000823"
     CDS             complement(36651..37145)
                     /gene="napF"
                     /locus_tag="OO332_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5941404.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37553..38041
                     /gene="eco"
                     /locus_tag="OO332_000824"
     CDS             37553..38041
                     /gene="eco"
                     /locus_tag="OO332_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5941405.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38191..39222)
                     /locus_tag="OO332_000825"
     CDS             complement(38191..39222)
                     /locus_tag="OO332_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5941406.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39456..39815)
                     /locus_tag="OO332_000826"
                     /pseudo
     CDS             complement(39456..39815)
                     /locus_tag="OO332_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40033..41676)
                     /gene="yojI"
                     /locus_tag="OO332_000827"
     CDS             complement(40033..41676)
                     /gene="yojI"
                     /locus_tag="OO332_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5941407.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41752..42346)
                     /gene="alkB"
                     /locus_tag="OO332_000828"
                     /pseudo
     CDS             complement(41752..42346)
                     /gene="alkB"
                     /locus_tag="OO332_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42346..43410)
                     /gene="ada"
                     /locus_tag="OO332_000829"
     CDS             complement(42346..43410)
                     /gene="ada"
                     /locus_tag="OO332_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5941408.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43484..44539)
                     /gene="apbE"
                     /locus_tag="OO332_000830"
     CDS             complement(43484..44539)
                     /gene="apbE"
                     /locus_tag="OO332_000830"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5941409.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44651..45772)
                     /gene="ompC"
                     /locus_tag="OO332_000831"
     CDS             complement(44651..45772)
                     /gene="ompC"
                     /locus_tag="OO332_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5941410.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46510..49182
                     /gene="rcsD"
                     /locus_tag="OO332_000832"
     CDS             46510..49182
                     /gene="rcsD"
                     /locus_tag="OO332_000832"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5941411.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49199..49849
                     /gene="rcsB"
                     /locus_tag="OO332_000833"
     CDS             49199..49849
                     /gene="rcsB"
                     /locus_tag="OO332_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5941412.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50049..52898)
                     /gene="rcsC"
                     /locus_tag="OO332_000834"
     CDS             complement(50049..52898)
                     /gene="rcsC"
                     /locus_tag="OO332_000834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5941413.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(53173..53949)
                     /locus_tag="OO332_000835"
     CDS             complement(53173..53949)
                     /locus_tag="OO332_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5941414.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53954..55603)
                     /locus_tag="OO332_000836"
     CDS             complement(53954..55603)
                     /locus_tag="OO332_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5941415.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55604..57868)
                     /locus_tag="OO332_000837"
                     /pseudo
     CDS             complement(55604..57868)
                     /locus_tag="OO332_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14137 a        15626 c        14226 g        13879 t
ORIGIN      
        1 cgaggtgaaa attttacagc gttgctgttc atcaagctga atgcagggcg tattggcggg
       61 cttgccatct ggcataccgg gaatagggct ggaaattgaa ggggcagtgc aacacgcccc
      121 gcaacccgga cggcattcca tacgttgttc tcttaaggtt aaccaccgga ttgatgagcg
      181 cgcacagtac cacctttttt gcaccagcaa aagtgcgaat accacttgcc cgaaaggccc
      241 atcgcgagta ctttgtcgcg atatttttga cattttcgac tacaggaatt tttcgatgcc
      301 aagagcgaac gaaattaaaa aaggtatggt actgaattac aacggcaaac tgctgctggc
      361 taaggatatt gatattcagt cgcccactgc ccgcggtgcc gctacgctgt acaaaatgcg
      421 tttttctgat gtccgtaccg ggctgaaagt agaagagcgt ttcaaaggtg atgatatcgt
      481 tgacaccgtg acgctgaccc gccgttacgt tgacttctcc tatgtcgatg gcaacgaata
      541 tgtctttatg gataaagaag actatacccc gtataccttc accaaagatc agattgaaga
      601 agagttgctg tttatgccag agggcggcat gccggacatg caggtgctga cctgggacgg
      661 tcaactgctg gcgcttgagc tgccgcagac cgttgatctg gaaatcgttg aaaccgcacc
      721 aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg gcgacattga gcactggtct
      781 ggtgattcag gtaccagaat acttaagccc gggcgaaaaa attcgtatcc atatcgaaga
      841 acgccgttat atggggcgtg ctgactaact tcagccgcat gcagaaaagg gatagctcag
      901 gctgtccctt ttttaattta ttataccaat cttctatttt gcgcttcctg aacaccttac
      961 gccctcaatt ttcactcgtt gattgatcgc cctcacactt catcgcatta acaatccaga
     1021 ccaatttcaa ttgctgtcat ataactttac actgtcattg ttaatttatc gttactaaga
     1081 cgtgactcct atgaagacaa ttgcctccgt tacgctcccg catcatgtac acgctccacg
     1141 ctacgatcgc cagcagttgc aatcacgtat cgttcatttt ggctttggcg cctttcaccg
     1201 cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc cagggcggcg actgggggat
     1261 ctgtgaaatc agcttgttca gcggtgatca actgatgagc cagctccgcg cacagaacca
     1321 tttatatacc gtgctggaga aaggtgcgga cggcaatcag gtgataattg tcggtgccgt
     1381 tcacgaatgc cttaatgcaa aactggattc cttagcggca attattgaga aattttgcga
     1441 gccacaggtg gcaattgttt ccctgacgat taccgaaaaa ggctattgta ttgacccggc
     1501 caccggtgca ctcgacacca gtaatccgcg gattattcac gatctacaaa cccctgaaga
     1561 acctcactcc gcaccgggta ttctcgtcga agcactgaaa cgccgccgtg agcgcggcct
     1621 tacaccgttt accgtgctct cctgcgacaa tattcccgac aatggccatg tggtgaaaaa
     1681 cgcggtgctg agaatggcag aaaaacgttc gccagaactc gccgggtgga taaaagaaca
     1741 cgtcagtttt ccgggaacca tggtcgaccg cattgttccg gctgcaaccg acgaatcact
     1801 ggcagaaatc agccagcatc tgggggtaaa tgatccctgc gcgattagct gcgaaccgtt
     1861 tatccagtgg gtggttgaag ataacttcat cgctgggcgt cctgcctggg aagtcgcagg
     1921 tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg aaactgcgta tgcttaatgg
     1981 cagccactct tttctcgctt atctgggtta cctctcagga ttcgcctata tcagtgattg
     2041 catgcaggat cgcgtatttc gctatgctgc cagaacatta atgctggatg agcaagcgcc
     2101 gacactgcaa attaaagatg tcgatttaac acaatatgcg gataagttaa ttgcacgttt
     2161 tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg atggatggca gccagaaatt
     2221 accgcaacgt atgctggcag gtattcgcat acatctgggg cgtgaaacgg actggtcgtt
     2281 gctggcatta ggcgttgctg gctggatgcg ttacgtcagc ggcgttgatg atgccggaaa
     2341 tgccattgat gttcgcgatc cgcttagcga taaaattcgc gaacttgttg cgggcagcag
     2401 cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa gttttcggtg atgatctgcc
     2461 agataacccg cattttgtgc aggccatcga acaagcctgg caacaaatcg tacaattcgg
     2521 cgcacatcag gcgctattaa accccctcaa aatttaacga tttctgcggt taaagcggat
     2581 gaagctcacc ttcgtccgct ctccccttct cttttctgcc ttttttagcc aggattaacg
     2641 ctcagttaac ttaccagagt attgcggagc cgttgtgacc aggaccaacc tcatcaccgg
     2701 ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg ttagcccata aagatcccaa
     2761 cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc ggaattgatg gtgctttgct
     2821 cgccgatagc ggcgcattgc tgaaagagat ccccggcggc tgcatgtgct gcgttaatgg
     2881 tttacccatg caggtagggt tgaatacctt actgcgtcag ggaaaaccag accgcttgtt
     2941 gatagagccg accgggctgg gccatccgaa acagctcctc gatctgttaa ccgcaccagt
     3001 ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt ctcgatccac gcctgctgct
     3061 ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag ctggctgccg cagacatcat
     3121 tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag caagcgctac agcgttggtg
     3181 gcagcaaaat ggtggcgatc gacaattaat tcacagtgag catgggaaag ttgacggtca
     3241 tcttctggat ttgccgcgtc gcaatttagc cgagttgccc gccagcgccg cgcattctca
     3301 tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg ccagagcatc aacgctggcg
     3361 tcgcagtctg aacagcgggc aaggatatca ggcctgcggc tggatattcg acgctgatac
     3421 ggtattcgac accattggca ttctggaatg ggcgcgactt gcaccggtgg aacgcgtcaa
     3481 aggcgtgctg cgtattcccg aagggctggt acgaatcaac cgtcagggcg atgacctgca
     3541 cattgaaacg caaaacgttg cgccaccgga cagccgtatt gagctgattt ccagcagcga
     3601 agctgactgg aatgccttgc agagcgcgct gttgaagctt cgtttagcga ctaccgcgta
     3661 aggttgcctg cgtttttcag taagataatt agagaaaata tgattaaaaa tttgccgcaa
     3721 atagtgttgt tgaatattgt cggccccgcg ctgtttcttt cctggtatat tcccgttaat
     3781 catggattct ggttgccgat tgatgcggat attttttatt tctttaatca gaaactggtc
     3841 gaaagtaagg cctttttatg gctggttgca ttgactaaca atcgcgcctt cgacggttgt
     3901 tcactgctgg cgatgggtat gttgatgctg agtttctggc tgaaagaaaa cgcccctggc
     3961 agacgacgta tcgtgattat gggtctggtc atgctattaa ctgcagtggt attaaaccag
     4021 ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa cattgacttt taccgatatt
     4081 aaccgcgtca gcgaactgct ctctgttccc acgaaagatg cctcacgaga tagctttccc
     4141 ggcgatcacg gcatgatgct gcttattttt tcggcattca tgtggcgtta tttcggcaaa
     4201 gttgcaggcc ttatcgccct tattattttt gtggtttttg catttcccag agtaatgatt
     4261 ggcgcacact ggtttactga catcattgtc ggttcgatga ccgtgatatt gatcggtttg
     4321 ccctgggtgt tgctgacgcc attaagtgat cgattaatca ccttttttga caaatcacta
     4381 ccaggaaaaa acaaacattt ccaaaacaaa taactcacag taattaacat catcagggtt
     4441 atttttatag tgaggataat cctgatgatg cgcaccgtgc tttcatctat cgaacgcaaa
     4501 aatcattctc taagtaaatg aatggattgc atgcgtttca ctcaattgta ctttaattga
     4561 ccaaccccgc ttattaactt tctgtatcac tttttcttat aaaaaatcat gtaaaaccgc
     4621 tcgccaagac cgcaccaatc gggtaatctc gaactcgttt tgcctcggcg gtagattatc
     4681 ctcacagcat ataattttgt gcgttagtcc acagatttgg ccttaaggaa ttgtttcaac
     4741 atgcccaggt aattagtctc gtgtcgcctg gcattttttt ataacgatat ttgtcgttaa
     4801 ggacttcaag ggaaaacaaa caacatggtc aaatctcaac cgattttgag atatatcttg
     4861 cgcgggattc ccgcgattgc agtagcggtt ctgctttctg catgtagtgc aaataacacc
     4921 gcaaagaata tgcatcctga gacacgtgca atgggtagtg aaacatcatc actgcaagct
     4981 tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg taaaatcgcg aattatggat
     5041 cagtatgctg actggaaagg cgtacgttat cgtctgggcg gcagcactaa aaaaggtatc
     5101 gattgttctg gtttcgtaca gcgtacattc cgtgagcaat ttggcttaga acttccgcgt
     5161 tcgacttacg aacagcagga aatgggtaaa tctgtttccc gcagtaattt gcgtacgggt
     5221 gatttagttc tgttccgtgc cggttcaacg ggacgccatg tcggtattta tatcggcaac
     5281 aatcagtttg tccatgcttc caccagcagt ggtgttatta tttccagcat gaatgaaccg
     5341 tactggaaga agcgttacaa cgaagcacgc cgggttctca gccgcagcta ataaaccgtt
     5401 tggatgcaat cccttggcta tcctgacgag ttaactgaaa gcactgctta ggcagtgctt
     5461 ttttgttttc attcatcaga gaaaatgatg tttccgcgtc ttgatccagg ctatagtccg
     5521 gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa ttaacggaat catgttcata
     5581 cgcgctccca attctggacg taagctcctg cttacctgca ttgtagcagg cgtgatgatt
     5641 gcgatactgg ttagctgcct tcagttttta gtggccaggc ataagcacga agtcaaatac
     5701 gacacactga ttatcgacgt acaaaagtat ctcgatacct attttgccga cctgaaatcc
     5761 actactgacc ggcttcagcc gctgacctta gatacctgcc agcaggctaa ccccgaactg
     5821 accgctcgcg cggcgtttag catgaatgtc cgtacgtttg tgctggtgaa agataaaaaa
     5881 acattctgtt catctgcgac tggtgagatg gacattccac tcaatgaatt gattccggcg
     5941 ctcgacatta ataaaaacgt cgatatggcg atcttacccg gcacgccgat ggtgccgaac
     6001 aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga aaaatagcgg tgtgtttgcc
     6061 gctctgaatc tcaacctgac gccttctctc ttttatagtt cacggcagga agattacgat
     6121 ggcgtcgccc tcattattgg caatactgcg ctatctacct tttcttcacg tttgatgaac
     6181 gttaatgaat taaccgacat gccggtccgt gaaactaaaa ttgcgggcat tcctctgacc
     6241 gttcggcttt atgcggatga ctggacatgg aacgatgtgt ggtacgcatt tttactgggc
     6301 ggcatgagtg gaactgtcgt tggcctgctt tgctattacc tgatgagcgt acgtatgcgc
     6361 cccggcagag aaatcatgac cgccatcaag cgcgaacaat tttacgtggc gtatcaaccg
     6421 gtggtggata cacaagcttt gcgggtaacg ggcctggaag tactgctacg ctggcggcat
     6481 cctgtagcgg gagaaattcc cccggatgcc ttcattaact ttgccgaatc gcaaaagatg
     6541 attgtgccgc tgactcagca cctgtttgag ttaattgccc gcgatgccgc agaattagaa
     6601 aaagtactgc cggtaggcgt caaatttggc attaacattg cgccggacca tctgcacagc
     6661 gaaagcttta aagcagatat ccagaaactg ctcacttccc tgcccgcaca ccatttccag
     6721 attgtgctgg aaattaccga gcgcgatatg ctgaaagagc aagaagccac acaactcttc
     6781 gcctggctgc acttggtcgg cgtagaaatt gctattgatg acttcggcac cgggcacagc
     6841 gcgcttatct atcttgagcg ttttacgctc gattatctga aaattgaccg tggatttatc
     6901 aacgccatcg gtacggaaac gatcacttca cccgtacttg acgcggtgct gacgctggcg
     6961 aaacgtctca atatgctgac agttactgaa ggggtcgaaa cgccggaaca agcgcgatgg
     7021 ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga ttagtcgccc gttaccgctg
     7081 gacgattttg ttcgctggct gaagaaaccg tatacgccgc agtggtaagg tgtgcttacg
     7141 tcccttatta ttcatagtga aagcatgccg gattgcggct aatgatgagt aaaaggaaat
     7201 ccgttgcaga tgattgtgcg catactgctg ctgtttatcg ctctgttcac cttcggtgcg
     7261 caggcgcagg ctatcaagga aagctatacc tttgccgtgc tgggcgaacc ccggtacgcg
     7321 tttaatttca accattttga ttatgtgaac cccgccgcgc caaaaggtgg gcagataacg
     7381 ttgtcagccc tcggtacctt cgataatttc aaccgctatg cactgcgcgg caacccgggc
     7441 gcacgcaccg agcagctgta cgacacgtta tttacgactt ccgatgacga accaggcagt
     7501 tattacccgc tgattgctga aagtgcacgc tatgctgacg attattcctg ggtggaggtc
     7561 gctattaatc cgcgcgcccg ttttcatgat ggttctccca ttactgcccg cgatgtagag
     7621 tttacttttc aaaaatttct gaccgaaggc gtgccgcaat ttcgtctggt ctacaaaggc
     7681 accaccgtca aagccattgc gccgttaacc gtgcgcattg agttagctaa acccggcaaa
     7741 gaagatatgt tgagtctgtt ttcgctgccg gtatttccag aaaagtactg gaaggatcac
     7801 aaacttagcg acccgctcgc cacgcctccg cttgccagtg gtccgtacca cgttacgtcc
     7861 tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag attactgggc agcaaactta
     7921 ccggtaaacc gtggacgctg gcatttcgac accattcgct acgattatta cctcgatgat
     7981 aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt tgcgtatgga aaacgacgct
     8041 aaaaactggg ccacgcgtta taccggtaaa aatttcgata aaaaatacat catcaaagat
     8101 gagcaaaaga acgaatcagc ccaggatacg cgctggctgg cgtttaatat ccaacgtgcg
     8161 gtattcagcg atcgccgggt gcgggaagca atcactctcg cctttgactt tgaatggatg
     8221 aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca gttattttca aaataccgaa
     8281 tacgcggcca gaaattaccc cgacgccgcg gagctggtgc ttctggcacc aatgaaaaaa
     8341 gatctaccgc cagaagtctt cacacaaatc taccagccgc cggtatctaa aggcgatggc
     8401 tacgatcgtg acaacctgtt aaaagccgac aaacttctca acgaagcggg ctgggtgctg
     8461 aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc tcagctttga attattgctt
     8521 cccgcaagca gcaatagtca gtgggtattg ccgttccagc acagcctgca acggctgggt
     8581 atcaatatgg acattcgcaa ggtggataac tcgcaaatca ctaaccgcat gcgcaatcgc
     8641 gactatgaca tgatgccgcg cctatggcgg gcgatgccgt ggcccagttc cgatttacag
     8701 atttcctggt catcggaata tatcaattcc acttataatg cccccggcgt gcaaagcccg
     8761 gttatcgact cgctgatcaa ccaaattatt gccgcgcagg gaaataaaga aaaattactg
     8821 ccgttggggc gagcactgga tcgcgtatta acgtggaatt attacatgct gccaatgtgg
     8881 tacatggcgg aagaccgtct cgcctggtgg gataaattct cccaacccgc tgtacgccct
     8941 gtttacagcc taggtatcga tacctggtgg tatgacgtta ataaagcggc caaactgccg
     9001 tcagccagac aacagggaga gtagatgggc gcttacctga ttcgccgtct gttgctggtg
     9061 atcccaacgt tatgggctat tatcactatc aactttttca tcgtgcaaat tgcgcctggc
     9121 ggtccggtcg atcaggccat cgccgccatt gagtttggta atgccggagt attacccggc
     9181 gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg gtgtcggcaa tatcagcgac
     9241 agtaattacc gtggcggacg cggattagat ccagaagtga tcgctgagat cactcatcgc
     9301 tacggtttcg ataagccgat ccacgaacgt tacttcaaaa tgctctggga ctacatccgc
     9361 tttgattttg gtgatagcct gtttcgcagc gcgtcggtgc tgacgctgat taaagacagt
     9421 ctgccggttt ccatcaccct cggattgtgg agcacgctga ttatctatct ggtgtcgatt
     9481 ccgttaggca ttcgcaaagc tgtttataat gggagtcgct ttgacgtctg gagtagcgca
     9541 tttatcatca tcggctacgc cattccggcc tttttgtttg ccatcctgct gattgtcttc
     9601 ttcgcgggcg gcagctattt cgacctgttc cctctacgcg gcctggtttc cgctaacttt
     9661 gattcgctgc cgtggtatca gaaaatcacc gattatctgt ggcatatcac gctaccggtg
     9721 ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc tgaccaaaaa ctcattcctt
     9781 gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga aaggggtaag tgagaaaaac
     9841 attctctgga aacatatgtt ccgcaacgcc atgctgctgg tgattgccgg ttttccggcg
     9901 acgtttatca gcatgttttt taccggctcg ctgctgattg aggtgatgtt ttcactcaat
     9961 ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg attatcctgt aatgtttggt
    10021 accttgtata ttttcaccct gattggcctg ctgctgaata ttgtcagtga tatcagctat
    10081 acgctggttg atccgcgtat agattttgag ggacgttaat gtcgcgactc agccccgtca
    10141 atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg ctactggtcg ttatggattt
    10201 tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat cgctaacgat aaaccgttgc
    10261 tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa aaactacagc gaaagcgatt
    10321 ttggcggccc gctggcaagt caggctgatt atcaggaccc gtggctgaaa caacggctgg
    10381 aaaataacgg ctgggtactg tgggcaccga ttcgctttgg tgctaccagt atcaactttg
    10441 ctaccgataa gcccttccct tctccaccct cccggcaaaa ctggctggga acggatgcaa
    10501 acggcggcga tgtgctggca cgcattctct atggcacgcg gatctcggtt ctgtttggcc
    10561 tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc gggggcgcta caaggctatt
    10621 acggcggtaa agtcgatctc tgggggcaac gctttattga agtatggtcg gggatgccga
    10681 cgctgttttt gattatttta ctttccagcg tcgtacagcc taacttctgg tggctgctgg
    10741 caattactgt cttgtttggc tggatgagtc tggtcggcgt ggtgcgggcg gagtttttac
    10801 gtactcgtaa tttcgactac attcgtgcgg cacaggcgct tggcgtcagc gatcgcagta
    10861 tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac cctcaccttt ttaccgttta
    10921 ttttatgtag ttcgataacc accctgacct cgctcgattt cctcggcttc ggtctaccgc
    10981 tcggttcacc gtcactcggt gaactgctgt tacaagggaa aaataacctt caggccccgt
    11041 ggcttgggat caccgccttc ttgtcggtgg cgatattatt gtctttgctg atctttattg
    11101 gtgaagccgt ccgcgacgca tttgatccta ataaggcggt gtagcatgac gcaaactctg
    11161 ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc aaaccgtacg tacagtagtc
    11221 aatgatgttt cactacagat tgaggctggc gaaacgctgg cgctggtggg tgagtcaggt
    11281 tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc tcccttcccc gccggttgaa
    11341 tatctctccg gcgatattcg ttttcatggc gaatcgctgc ttcatgccag cgatcaaacg
    11401 ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc aggaaccgat gctgtcgtta
    11461 aatccattgc ataccctgga aaaacagctt tatgaagtgc tttcactcca ccgcgggatg
    11521 cgtcgggaag cggctcgtgg cgaaattctt aactgccttg atcgcgtcgg tatccgccag
    11581 gcggcaaaac ggctgacaga ttatccgcat cagctctccg gcggcgaacg ccagcgggtg
    11641 atgattgcga tggcgctgtt aacgcgaccg gaattattaa ttgccgatga accgaccacc
    11701 gcactggacg tctctgtcca ggcgcagatt ttacagctgt tgcgcgaact gcaaggcgag
    11761 ctgaatatgg gcatgctgtt tattactcat aacctcagca ttgtcagaaa actggcccac
    11821 cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa ataacgccgc tacgctattt
    11881 gcctcaccca ctcatcctta cacacaaaag ctactcaaca gtgaaccgtc aggcgatccg
    11941 gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg aacagcttca ggttgccttc
    12001 cccattcgca aagggatttt gaagcgcatt gtggatcata atgtggtggt gaaaaacatc
    12061 agttttacgc tacgggcggg tgaaacactg ggtttagtgg gcgagtctgg ttccgggaaa
    12121 agcacgacgg gactggcgct gctgcgactg attacctctc agggcagcat cgtctttgac
    12181 ggtcagccac tgcaagattt aaatcgccgc cagctgttac ctactcgtca tcgtattcag
    12241 gtggtatttc aggatccaaa ctcctcgctc aacccacgac tcaacgtttt gcagattatt
    12301 gaggaaggct tacgggttca ccagccgacg ctttctgccg cacaacgcga acaacaagtg
    12361 atagccgtga tgcatgaagt gggattagat cctgaaacac gccaccgtta tccggcggag
    12421 ttctctggtg gtcagcgaca acgtattgcg attgccaggg cattaattct taagccctcg
    12481 ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa cggtacaggc gcaaatattg
    12541 acgcttttga aatcattgca acaaaagcat caactggcct atttgtttat cagccacgat
    12601 ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac tgcgacaagg ggaagtagtg
    12661 gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc aggagtatac gcgtcagtta
    12721 ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa aagggttcgg caattgccac
    12781 accaaaattt ttcaatcggc atgttgcagc aaactcgtcc tggcgattta caaacaggca
    12841 cggctcacct tcacactcca acaccgaaca tggaacttcg atgtcgctta acgcctggct
    12901 tagtttatac atcaccgacc atgcattatc accatccggg ctaagcaatt taagcgccac
    12961 caggctgttg tcaccgcacg gtccattttg cggaatcggt tcaaccttcg aacctgcgaa
    13021 accagcagac cagcgatagt tctgcggcag tcgatggacg attgagagtt gtaatgaagt
    13081 cacttatttt ccccggaagt acattcgctt cacaatttgt ttacatcaat tttaacacat
    13141 catcaacaat ccgtctttat acagatcgta aagattgatg cctcgcctgc gtggcctcta
    13201 tggctctgat ttaagtaata tctgcgggcg tggtcacgtt tgcggggcta tatgtacccg
    13261 tttctgcgat ctaactcaac ctttttaact acaatgatgt gactttttac ataaattgat
    13321 tttacataaa ataaacatat atcggggaaa tgatagattt gtggttgaca tgcatcaaca
    13381 aaggaagcct tttaccttcc tcgttgtgca atagatcacc gttttttcgg ccgactggcg
    13441 tacagacaga agagaatgga gctggttgcg cagaatgcaa ttgaccaaat catcggccat
    13501 gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa ttgcccctat gccaaaacgg
    13561 aaggttcctg ccagcgaaga tgccgttccc gccatatggg gaaactcatc aagaatgacc
    13621 gccatcgcat tggatgacac catcgacacg cagcccacaa acaccgcaac gccaaccacc
    13681 agcgaccaga atcccagccc cagcagcgca ctgatgacca tccacgctgc cataataaat
    13741 tgtatccaca accccgagcg gaacatattt aacgcgccaa tgcggcggac gaagcggctg
    13801 ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg caaagtaata accaaagttt
    13861 tccggcgcga cgtggttaat ttcaatataa acaaacggtc cggcgcttaa gaatgagaac
    13921 atcccggcaa agctgaaacc actggcaagc atgtagctca ggacacgttt atggcggaac
    13981 agcgccgcaa agttaccaat agtggtacga atgtgaaatg gctgacgacg ctccggtggt
    14041 aaggtttctt taatcaggaa gaaaatcatt gccgaagcca gaatcgccgc taatgccagg
    14101 atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac tatcggtgcc
    14161 atcagcagtg caatggtcgt caccagcatg acaaacgaca tcatccgcga gaactcttct
    14221 ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc cgcagccagc
    14281 ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc acacgccacc
    14341 gcggcggcgg caaacaccag cgtcccgcca agaaccaccg gcttacgccc gaagctgtct
    14401 gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt actgagggtc
    14461 atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa ttaccggtag cgcgggcaga
    14521 tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa aacaatagca
    14581 aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa aagttagacg
    14641 acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa atcagcatcc
    14701 agcgtaatac cgccaatacg ttcacgatgc agctcaacca cgtggttacc cacggcggcg
    14761 aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac ctgcgttggg
    14821 gtaatcactt caagcaccgc cggcttagtg agatcttttt cgttatgcag ctgcacgcct
    14881 ttagcaaatt gctctgccgt atcgtcagct acaggtgatt ccagtgtcac cagataggtc
    14941 ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc atcagtcatc
    15001 agcaccagac cggtggtatc gatatccaga cgtcctgcgg cgtgcagttt ccacgctacc
    15061 ggttcatcca gaaaatagag caccgtcggg tgatccgggt cgtccgtgga gcaaacatag
    15121 ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag cgggttgcca
    15181 tcgtaagcga catcatgttc agggagcagt ttgaacgctg cattacgaac gatttcgcca
    15241 tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct aacgccgagt
    15301 tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa aaacggaggt
    15361 cgggtaatga cccgaaaatc tgaactgttg tctgcccagt atagcggtct aataacttcc
    15421 ctcaagggaa aaagaaccgt ggcatactat tagcagaata atctacctac gcgaaaccat
    15481 gatttttaca ctccgcccat atcagcaaga agccgtggat gccacgctca accattttcg
    15541 tcgtcataaa acccctgccg ttatcgtgct gcccaccggc gcaggtaaaa gcctggtgat
    15601 agcggaactg gcacggctgg cgcgtggtcg cgtactggtg ctggcacacg ttaaagaact
    15661 ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata tttttgccgc
    15721 cgggctaaag cgcaaagaga gccacggtaa agtggtattt ggcagcgttc agtcggtcgc
    15781 ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg aatgccaccg
    15841 tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga ctaaagtgaa
    15901 tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca aaggctggat
    15961 ctaccagttc cattatcacg gcatggtacg cggcgatgag aaagcccttt tccgtgactg
    16021 catttatgag ctgccgctgc gttatatgat taaacacggc tatctgacgc cgccagaacg
    16081 actggatatg ccagtagtgc aatacgattt cagccgcttg caggcacaga gtaacgggct
    16141 gttcagcgaa gctgatctca accgtgagct gaaaaaacag caacgcatta cgccacatat
    16201 catcagccag atcatggagt tcgccgaaaa gcgcaaaggg gtaatgattt tcgccgccac
    16261 cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgctg cactgattac
    16321 tggcgacccc cccggcgctg agcgcgatgt gttaattgaa gattttaaag cccagcgttt
    16381 tcgctatctg gtcaacgtcg cagtactgac caccggattt gacgccccgc acgtcgatct
    16441 tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg tcgggcgcgg
    16501 tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg gtaatcctca
    16561 cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg ttccggtaca
    16621 ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga ccgccgacgg
    16681 gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg acgacggtca
    16741 tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta acgcagaaaa
    16801 tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc cggatgatat
    16861 gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg gcatgtcttt
    16921 gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg atgaagacgg
    16981 cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc cagcgtaccg ccttcgagca
    17041 gctttttatc cgcccgcata cgcgcacacc gggcatccct ctgcgctgga tcaccgccgc
    17101 cgatatcctc gcccagcaag ccttactgcg acatccggat tttgtcgtcg cccgcatgaa
    17161 aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac gaaggtcgtt ttcgtcgggc
    17221 gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg agtatagaat
    17281 ctcgcccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc gcctgtagca
    17341 ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt aaagagcagg
    17401 gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc atctacggtg
    17461 gcaaagaagc accgctggct atcgagctgg atcacgacaa agtcatgaac atgcaagcta
    17521 aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa atcaaagtta
    17581 aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc gacttcgttc
    17641 gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt ttgtatctgc
    17701 agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg ttcacgccgc
    17761 atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg ctgcaattgg
    17821 tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat cggttgctgg atcccagaag
    17881 atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc gctaccggca
    17941 aacttcgtca actgacgcag tgtgctacgg tctgccggga agctctcttc cagttcgtag
    18001 cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag ctctttcgac
    18061 agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta gctataaacc
    18121 tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt gaaatcatca
    18181 accgcttgca acagcccacg gttttgcgct ttcgcgttca atccttcgct ggccccgagg
    18241 aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa agtgagatag
    18301 cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac aatatccgca
    18361 tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac gcgcatactg
    18421 ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg gtagtggcaa
    18481 aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg caggcgaccg
    18541 gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa gcgcagcgtc
    18601 tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc gctatagacc
    18661 cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa tgaatcgcgc
    18721 aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc aatctggttg
    18781 atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc accgccggtt
    18841 aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga gaatgattta
    18901 acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct ttcatcaact
    18961 aactgatttc gatttatgcc acaaatttcc cgctacagta atgaacaggt tgaacaattg
    19021 ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct ttccctgatg
    19081 gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc ccaacgccag
    19141 gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga caaagcgcac
    19201 taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg aaaaagtctc
    19261 ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt cgctcgtcat
    19321 tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc gcatttattc
    19381 ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact tgctgatcct
    19441 cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt ccgtcattct
    19501 ggcgacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc gtttccatct
    19561 ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg agatggcgcg
    19621 cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac tggtgtttgc
    19681 gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg cgcgcccgct
    19741 ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct gggccgatgc
    19801 caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt acccgatgac
    19861 ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg caggagtatc aacgccgtct
    19921 tattgagcaa ggtaatccag acaccgtttc cgtacagtat ccgttaagcg aactgcgcta
    19981 tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc tgaactactc
    20041 acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata tttcgttcac
    20101 gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt tctatggcat
    20161 ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat taattactgc
    20221 gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta ccagcccgct
    20281 gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc aatctgacga
    20341 tcagaccgcc acgcagtgga tcaactggct gggccgctac gcacaagaag ataaccgctg
    20401 gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc aggcatttgc
    20461 acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaacgcg tgctcaatgc
    20521 actgcgtgat tctggcaaac tggacaatac ggtggtgatt atcactgccg gtcggggtat
    20581 tccactgagc gaagaggaag aaacctttga ctggtcccac ggtcatctgc aggtgccatt
    20641 agtgattcac tggccaggca cgccggcgca gcgtattaat gctctgactg atcataccga
    20701 tctgatgacg acgctgatgc aacgcctgct gcatgtcagc acacctgcca gcgaatattc
    20761 gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg ctgcggataa
    20821 cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata acggtaaata
    20881 ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt taagtttgtt
    20941 attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta taaatcagtt
    21001 agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc cacgagtcgg
    21061 cacgtagcgc agcttggtag cgcaccgtca tggggtgtcg ggggtcggag gttcaaatcc
    21121 tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt acggttggtt tttttatatc
    21181 tgcaattaat tcgataaaca gaccgtgaca catcacagcc tgtttatttt ctgttatcag
    21241 aacgtccaga ccacacccgc ctgagtattc cacggcgatt ctacccctgc gccattgcca
    21301 tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat cccagcctga
    21361 taaacgccac tggttccagc cacatcatta ttaaatttgc catcgtcatt aactttcacc
    21421 tggttagaat cggcgtattc ctgacgcacg gcggctttca gccagggttc cagcgtcgta
    21481 ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg taatatccgg
    21541 gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc atctgtggta
    21601 aagccggtaa aggccagata gggtctaaca ctccacaatc cgtcaaccca acggaacccg
    21661 ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt tgccccatta
    21721 ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc atcaacatag
    21781 gcaccgttct gatgctccca accggcataa gcccccaggg tatagctatc gatattacct
    21841 tttccgccgc gatcaaaacc aatatcagaa tgagagtaac caaagaacaa gccgcgaatt
    21901 gtactgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc cgtcaatgtt
    21961 tgctcaaaac cagctcccgc atcagtggtc acgttgttgc gggtgttaat tgccgaactc
    22021 cacatcaccg tatcgtaact aacgcctttt acgctaccaa gacgctcacg cacggtgtcc
    22081 agttctgcat caaataccag cggttgtgcg gctgccatgt tcagcacatc ggtggttgaa
    22141 ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt atccagcaag
    22201 gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt aaatgcagca
    22261 tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg ctgccgggct ggcaccggtg
    22321 tccgtcacga atattttgaa atcacctgtt gcctgaccgg tgacgttgag ctgatcgctc
    22381 tgatgattgg ccatatcggt acgcatataa aaattgccgt ttcccgacag ggtattggtg
    22441 gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg aacgagtcac tggctcagcc
    22501 atcataatca catttgcgcc ggacatcgca aggttggcta agttgaggcg agtgttttgg
    22561 ttcgtcgtag tgatatcggc aatgatggtc catgcgctat tttcgagcga aacgtccgct
    22621 ttgctggtat ccacggcacc atgcgttcta aaagaagcgc cctcagaaac cactacctta
    22681 cccaccaaca tagcgggtgc ataatttatt tgcggcttca tgacctcaag aatgccatca
    22741 ttccctctga ctgacacgtt aaccattgtg ccagcccaga cattagcgcg gccattgttg
    22801 attaccatat tttcagagat ggcttttgag gaatacgtga tacttccatt ctggtctgat
    22861 cgaccgagat catataccgc atttgcctgc actttcgtac cagtatcctt tcccagcgat
    22921 tgcatagtgg catgctcatc aaggatagtg tcaatagcct gcgtgtcctc cataacaatg
    22981 agcccggaac catcatccag ttcataattt tttgacacac catcctttat actaaattgg
    23041 cctttactgt ttgtaccact cacttccagc gcattcgttg agacaattaa tttcccgcct
    23101 gcttttttat caacgccagt tgcagtcccg ccatccataa cctccagtaa gccgccacta
    23161 tccacagtag tattataagc gaagtcattt tcttcaactc gcaaacttcc gccgttttcc
    23221 aacaacatat tattagcaat accattcttg atatcgaact gaccgagacg gtttgttccg
    23281 aatacctcca tggcccgcgt ggttgtttta atagcaccgc ctgctttctg atctaccgca
    23341 agcgcaaatc ccccctcttt aacctccagt agtcctccct tttcgatagc ggcaaaaacg
    23401 gtccccccat ctttttcaac gattaagcgc ccgcctttaa tcgtggtatc attcgctggg
    23461 ccataaacct gtagcacacc attattgagg gtagtctggt ttgccgcgcc acccatttga
    23521 acaatttgat aaccatcatt taatactgag tattctgcgg taccattcat ttcaatgtat
    23581 tgataccctc cgttaatttc agtattacta cttacaccaa attcttttat atgctgttct
    23641 ccgccaggat tgattttcgt attattggcg gtcccataaa ccagctggat atctttttcc
    23701 aggataacac catcaactgt ctcaccatac tcaactgttg atgccaatgc tatgggtgaa
    23761 tataacgctg tagccaccag aacagatatt gcggaacgac aaagagaaac agaaccagat
    23821 tgatgcattg agctttcatc ctatgaaatt aattgcagtt aaaagcattg ggtacagaaa
    23881 atacccatag ctccataccc ggagtcagtt tttaaaaact gtttaaagaa atgcacaagt
    23941 attgtgattg attttttagt tgtttttctt gatgagaagc tgatgcaaaa ttccgtcttt
    24001 ataatgaaaa tgatgccaaa gcgaacgaca aggttgtagt tttcactaca tgtccataca
    24061 taaaatgggg taacattcac gcgcctggta gcgttaccaa cgctacgctc aaacataatg
    24121 attctaataa aacctcagga gactactatg cctgaagcaa cgccttttca ggtgatgatt
    24181 gtggatgatc atccacttat gcgacgcggt gttcgtcagt tactggagct tgatcctggc
    24241 tttgaagtgg tcgccgaagc gggcgacggc gcgagcgcga tcgatctggc gaatagactg
    24301 gatatcgacg tgatcttgct ggatctcaat atgaaaggta tgagtggcct ggataccctc
    24361 aatgccttgc gcagggatgg cgttaccgcg caaattatta tcctgaccgt atccgatgcc
    24421 tccagcgatg tctttgcgct gatagacgca ggcgcagacg gttatctgtt gaaagacagc
    24481 gacccggaag tattgctgga agcgattcgt accggagcga aaggcaacaa agtctttagc
    24541 gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg gcgcggaaga agatcccttc
    24601 agcgtgctga cggagcgcga gctggatgtt ctgcacgagc tggcacaggg gctgtcaaat
    24661 aaacagattg catcggtgtt gaatatttcc gagcagacag taaaagtaca tattcgcaat
    24721 ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca ccattctgtt cctgcaacaa
    24781 cgcggggcac aataaaaata gcccgatgga tttatcatcg ggctgagatt tatgacaaac
    24841 gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg gatcgatcaa tttattgaat
    24901 ttacacaatt ttgtaggccg gataaggcgt tcacgccgca tccggcataa actaagcgca
    24961 ctttgtcatc agtctagccc gatggcatca ccatcgggcc tctttttatt tactctcctg
    25021 cggcgacaaa tgttgcatcg cctgcgcgat actacgttca atcaccgcac ggcgagtatc
    25081 gttggcaggt aagagtttca acatcatctc ccacgcggca acggcttcgc caaatcgctg
    25141 ctgctcaaag gcattaaacg catacatgct tagcacacgg atattgctat ggtccgttct
    25201 caccagctga cgtagcagtt caccgccgag gcggttgtcg ttgggatcag atgaacgagt
    25261 caacgcttca gcgtatccca gtgcggcatc gctgttcttc ggatcgaggc gatacgcagt
    25321 agcgtatgca tcggtggcga tactggcgtt acccagcgcc atgccaacgc ggcccaacat
    25381 aatccagcct tctatatctc ccggattttt ttgcagttga gtacgcatcc ccagcgcaag
    25441 gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg tccagcgccc tgtccagcaa
    25501 cgccggagcc tgtgccgtgg cctgctgcca gattttcacc tgctgataat tgccagtctg
    25561 gtagtagctg acgccagcca caattaacgc caccacaata cccggcagat aaacaacata
    25621 tccggcacgc ttaccttccg gcacgctttg ttcaggaaac gcttccggca ccacgcgtac
    25681 ccgacgccgc gagcgggcgt atatgaccca accgccaatg ccaatagcca ctactggcag
    25741 cacccacagc agcacggtca gcggcgttaa cggcggatcg taagtgacga agttgccgta
    25801 acgcgccacc atataatcga caatctcttt tttactttta ccttcctgca tcagttcata
    25861 cactttctga cgcaggtcgg tggcaatcat cgagttggaa tcggcaatgc tgttgttctg
    25921 gcatttcggg cagcgcagtt cttcagtgag ctgacggaac tgttgttcct gtgcttcatc
    25981 tttaaactgc aacacgtcga tggtcgccag cgctgagccg gagatcatca gcatcagcac
    26041 gcccaataaa aacctcattg tgcggcctcc ttactgtatt tctcccacag cggcttgatc
    26101 tcttcttccc agacgcgagg attgagatcg cccgcatggc gatagcgaat gataccgttg
    26161 ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga gatccagccc taacatgccg
    26221 tcgccatcaa acaggcttag cgcgtaagga ttgcccagct ctttcagcca gctgattgcc
    26281 ttctggcgat cgtctttata gttcatgccg accacgcgaa tgccctgcgc tgaaagctga
    26341 ttcagatatt gatgttccgc acggcaggtc ggacaccaag tcgcccagac gttaagcagt
    26401 actggtttgc cctgagtcag cacatccgcc tgataaaact gccccggatt gtccagtgat
    26461 tcgagacgaa acttcggcac aggcttgcca atgagcgccg attccagatt ggtcggatca
    26521 tccccttcgg cattacgcgc cagctgccac agcagcgccg cggcaatcgc caggaagata
    26581 atcaacggaa ttaacaatac tttgcgcttc atacggcctc cggcgcagtt ttttgcggac
    26641 tcacgcgctt acgatagcga ggatcaaaca gacacagcag tccgcccaac gccatcatca
    26701 gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta aagacgcacg gcccacgcgc
    26761 cgttttccag ctcttcaccg agcgcggcgt acaggtcacg cgtgatgccg ccgtcaattg
    26821 ccgcttcggt catcatcgac ccggcagtgt tgtaataacg tttttccgca tacagcaccg
    26881 tttccggttt accctctcgc gttacgccaa tagtcgccac accgccgcgc cagttcgggc
    26941 cagtcacctc tttgacatca cggaaggtga agcgatattc atgaatatcg acgctatcgc
    27001 cggacttcat gcgcacatca cgctcaacgc tataattctg gctaaaggca atgccaacaa
    27061 ttgtcactgc cagccccagg tgagccgcca ccatccccca ataactgaag gtggttttcg
    27121 tgccgcgtga aatacgcagc gcagcttccg cagttgccag caccgcaatc cagcaggcca
    27181 ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc gaacagccac ggcaacagca
    27241 gagacagcac cagcgtagag atgaaggcga taatcaataa attgcggatt ttacgtgggc
    27301 gatcgcgccc ccagcgcacc agaggaccga caccaagcag tagcgcaaac ggcaccatca
    27361 gccaggtaaa catggtgttg aagaacggtt cgccaatcga aatactgccc agtccaagtt
    27421 gcttgtgcac cagcggcagc agcgtcccca gcaacaccac cagcatcgcg gcgaccagca
    27481 aaacgttgtt cgctaacagc aaagattccc gcgaccacag cgcattgttt acgcgtgagc
    27541 gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc gccaatcacc agcaccataa
    27601 aggcgaggat aaacataccg cgcgccggat cagacgcaaa cgcgtgtacc gataccagca
    27661 cgccggaacg cacgaggaaa gtccccaaca gacacaacga gaaggcactg attgccagca
    27721 gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac cgccagtgag tgcatcagtg
    27781 cagtccccac cagccacggc ataaacgagg cgttttctac cggatcccag aaccaccagc
    27841 caccccagcc gagttcgtaa taggcccatg cggaaccgag cacaatgccg agcgtcagga
    27901 aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc ataagtgctg tccagacgcc
    27961 cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga gaaacccacg taccccatat
    28021 aaagcagagg cggatggaag atcagccccg gatcctgtaa cagcgggtta agatcgcgcc
    28081 cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt agaggtaaag agaatgaaca
    28141 gcaaaaagcc gacgctgacc atccccatta tcgccagtac acgggccaca atatccagcg
    28201 gaatacgctg actaaaaatc gccaccgcaa aggtccagcc gctcatcagc agcacccaca
    28261 gcagaagcga accttcatgc gcgccccagg tagccgccac gcgataccac accggaagct
    28321 gggtattgga gttgctggca acataggtga cggtaaagtc gttgaccacg aaagcattga
    28381 ccagcaccag aaatgcgcca gccacagaca taaacagcag ccaggcaaac aagcgggaag
    28441 acgccatcat gcgcgcatct ccgcgcgcca cgccccatag cggatacacg gacagcagca
    28501 gcgcaatccc taacgccaag cacagcagcc cgtttccaat ttccggcatc atgatgctgg
    28561 gtccttataa acactcgccg ggcgacggtg gttagcttcc atcgctttct caacttctgg
    28621 cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc gcgaggatat gattgccttt
    28681 ttccagctcg ccctgcacca caacgccctg cccttcacgg aacagatccg gcaaaatgcc
    28741 ttcgtaagag acatccactg agccttcagc atcgtaaatg gtgaaggtca ctttcagcga
    28801 attgggatcg cgctgcacgc tacccggcat caccatcccg ccaacgcgca gacgctgacc
    28861 gacttccggc atctgctgag tttcacgctt gccgtagaga atttcccccg gcgtataaaa
    28921 gagatcgata ttcgagcgca gcgcatatag caccagaccg atagtcagcg ccagccctgc
    28981 caacacggca caggcaatcc acaagcggtt tttacggcga atattcatgc agcctcctgc
    29041 tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt gcgccacacc acgcagaatt
    29101 gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca gcggaataac ggtcatcacc
    29161 accgccagcc agacaaaaaa ggcgtaaccg cccattgcga aaaattcatt ccaggaagca
    29221 aatgcagggg tcatttacgg cctcttttca gtatcagttc actcacccac ggacggcgtt
    29281 tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt cagcgtggca gacaggagca
    29341 ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc cggatcgata ctttgctgca
    29401 tccgcgttga tccctgatgc agggtgttcc accactccac ggagtaatga ataatcggca
    29461 gattcaccac gccaatcagc accaggatac ctgccgcacg gcccgccaga cggcggtcgt
    29521 cgaaggcgtg ccacagggca atcacaccca catacaaaaa cagcagcacc agttcagaag
    29581 tcagacgtgc atcccatacc caccaggtgc cccacatcgg ttttccccat gcagagccgg
    29641 taaccagggc aataaaggta aacacggcac caatgggggc catcgccgcc accgccaggt
    29701 tggccatttt catctgccag acaaggccaa taaacgccgc caccgccatt gatgcataaa
    29761 tgcccatcga ccagatcgcc gcaggcacat gcaggtagat aatgcggtag ctatttccct
    29821 gctgataatc agccggagca aagccgaatc cccagatcca gccgacggta agcacgacca
    29881 cactggcaat tgccagccac ggtataaacc agccacagat ttgatacagc cgtggtggga
    29941 tcgccagttg atgcagtgtt ttccacatag tttcgatacc agactcgaac aaaaatcagt
    30001 aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc gtcgcaaaag gacttaatgt
    30061 cgcggtgcct gccagcaacg cgcctaaaat tgccagatac ccatcaacgg gcaaatgcat
    30121 agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat gggatagtca gcggtaacac
    30181 cagtatgctg agcagcacac caccgcgctt aagtcccact gtcagcgcca cgcccggtgc
    30241 accgagaaag ccaagcgtag gcgttcccag cagcagcgtc agcgccatca cttgccagcc
    30301 ataaacatcc attcccagta gcattgctac cagtggcgaa aggatgagta acggcagacc
    30361 ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg gcgggcaagg gtaacggcaa
    30421 caacatcaat tgttcaagac tgccgtcctg caaatcgtca cggaacagtc gttccagcgc
    30481 cagcaaggat gaaagcagcg cagcaaccca gataatgccc ggtgcaatac gcgccagcag
    30541 ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt acaatcagga agaaccacag
    30601 cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc agttcaagac ggaaaatacg
    30661 ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc ggcgaatttt actttcagca
    30721 acgttgagcg gctggtgggt agtcagaatc acaatccccc cctgctccgt atgctgcgcc
    30781 atacgctggg tcagacgatc gacgccgtta acgtcaatcg cggtaaaagg ctcgtcgagg
    30841 atccataatg tggcacgggt caaccacaga cgcgctaaag cgacgcggcg ttgttgcccg
    30901 gcagaaagtt gatttaccgg aatatcttcg aatccggcaa gcccggcctg cgccaaggct
    30961 tccagacatt gtgcggcatc gccatcgcga tgataaaagt gcagattttc taacgccgtc
    31021 agccgggttt tgatccccgg ctgatggcct atccataaca ggttttgatg gtagctgtcg
    31081 cgtacctgat gcaagggctg cccttgccag agaacctcgc cagcgtcagg gcgagacaac
    31141 cccgtcagca aacggagaag cgttgtcttc cccgcgccgt tgctaccggt gatttgtacc
    31201 cactctcctg cgttcagcgt aaatgacaag ccactaaata aggttcgttc atcccgctca
    31261 caaagtacct ctctggcttc aagcataccc acgcaataac cctgttaaaa acctggctcg
    31321 acttcacgca tatcgggcag cttgtgcgcg atccctttat ggcaatcaat acaggtttgc
    31381 ccatctttca ccgcctggtc atgcatcttc gcggcaaccg atttctgggc ggttgtatcc
    31441 atatactcga agttgtgaca gttacggcac tcctgcgagt tattgtcctt catgcgccgc
    31501 cactcattct gtgccatcgt cagacgatga gcttcaaatt tctgcggcgt gtcaataacg
    31561 ccaaaaattt taccatacag ctctttactt gctttgagct tgcgtatcat cttcggcaca
    31621 aactcgtgcg gaacgtgaca atccggacag gtcgcacgga cgccgctacg gttgttgtag
    31681 tgcacggaat ccatgtattc ctgatacacc gtgttgcgca tttcgtggca gctaatgcag
    31741 aactcttcgg tattggcttt ttccatcccg gtgttaaagc ccccccagaa gacgatgccg
    31801 ccaacaaaac cgatcaacag cagcgtcccc agcgccagat ggctgggggt acgccaccat
    31861 ttccacaggc gcttaatcag accaggctta cggtcagaat ttcccataat aacctcttat
    31921 ttcccgtaac cttttgatgg ggtaaaggta ttccccacga ttggcgcggt atcggcctgc
    31981 ggtacgtgac attgcagaca gaaataacga cgcggagcca cttccgcgcc cactttgccg
    32041 tcgctgtcca taaagtgagt aggactgata cgcggcgcgc cagtggtgcg atagctttcg
    32101 acaccgtggc actgcaagca gcgattggta ttggtcgtta cctgataacc ttcaacgcta
    32161 tgcgggatca ttggcggctg attcacatag ttcagcggca tccgatcctg ctcttttggc
    32221 atccgaatgg ccccttcctg cgtcccggat acttccggcg actggctaaa atcgacgcca
    32281 ttagccgccc aaaccgcgcc gcttaccacc agggccagca tcgccgtcca ttgacacagc
    32341 gctttcttca ggtcatggct tttcatgatt tcgctcccga actccatcgt gtagttattg
    32401 taaatacatc ctcagaacag acatccacgc agcgaccgca cgtcatgcaa tcgcggctgg
    32461 tgacctgcac cgggctttgt tcatccagca ccggggcacg tagcacatgc ggttccgggc
    32521 aaacatgaaa acaatccata cagcggttac atttctgccg atcgctggcc gcaacggtaa
    32581 tcacaccttt gctacccagc acgccataca gcgcgccaac cggacaaatg tgcccgcacc
    32641 agccgtgttc aacgaccagt agatcaaata aaaacagcgc gagaataagc agcgcgccgc
    32701 tgccgaagcc cattaccagg ctacgcccca tcagagaaac ggggttgatc cattcccaaa
    32761 tgagcgtgcc agttaaggct gatcccacca gaatgaccac caacagcacg tagcgaatgt
    32821 ggcgagggat cgtcgcagac tgattgaggt caaagcgcct gcgtaaccag ttcgctaagt
    32881 cggtaatcgg gttcagcggg cagacccagc tgcaaaataa tcgctttccc gccaaggcgt
    32941 agagcacggt gataatcacc gcaccggtca acgccaccgt ggcaggcaga tgaccactgg
    33001 caagactttg cagcgtcatc agcggatcgg tcagcggaac ggtgtcgaat aacaggctac
    33061 tgctgtagtt gccgtgcaag atccacacac caaaccatgg accgctcaaa aacatcccca
    33121 gcacgaagaa ctgacaaagg cgacgtaaca ccagccaacg gtgactgcgc caccagcctt
    33181 ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt tgccattgtt cccctccagc
    33241 cagccgaagc ggtaatggtg acctaactcc cctttcgcca gtgacagcgg taacaccttg
    33301 attgccggtt gttccagcac gcatactttt tcgcacttac cgcaaccggt acaggcgtcg
    33361 ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggtac gcgtgttgcg ctccagctcc
    33421 aaggtgatgg cctcatcaat tttcgggcat tcgcgataac aaacatcgca gcgcagcccc
    33481 tgaaagttga gacagttttc ctggtccacc agtaccgcca gccccatccg cgcgtcgtcg
    33541 atcgattcaa tctcacgatc cagcgcaccg cttgggcaca ctttggcgca cggaatgtcc
    33601 tcacacattt cgcaaggaat atcccgtgcg acaaaatatg gcgtgcccgc cgacagacca
    33661 gaggccagcg tcgccagttt taaggtgtcg taagggcaag cctgaacaca ctgaccgcaa
    33721 cgcacacagg cactggcaaa ggcgttctcg tttatggctc cgggcgggcg caaccgcacg
    33781 ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca cacccacggc agccagcccg
    33841 cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac cattttgagg tttcgctgac
    33901 cgggacataa tggcttacac cttctccagt ttgaccgcgc acttcttgaa gtccgtctct
    33961 ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa ccagctgtgc ggcgtcgaag
    34021 aacggcatgt acaccaggcc ctgtggcgga cggttacgac cgcgcgtttc aacaatcgag
    34081 atcacttcgc cacggcgaga aaccactttc actttgtcgc cacggcgcag atcgcgcgct
    34141 ttcgcatcca gcgggtgaat aaacaagacc gcttccggga aggcgcggtg cagttccggc
    34201 acacggcgag tcatactgcc ggtgtgccag tgctccagaa cgcgtccggt agagagccac
    34261 aggtcgtact cttcatccgg tgcttccgcc gccggttcga acggcagtgc gaagatcacc
    34321 gctttgccat ccggtttacc gtagaactta tagccttcgc ccgctttcac gtacgggtcg
    34381 ttaccttcgc tgtaacgcca ctgtgtttct ttaccattca ccaccggcca gcgcagaccg
    34441 cgcgctttgt ggtagtcatc gaacggtgcc agatcgtgac cgtgaccgcg accaaaccat
    34501 gcgtactctt cgaacagccc tttttgcaga tagaagccca gctcgcggga ttcatcgttc
    34561 agctgatctt ccgccagttc ggataccggg aatttgctca cttcgggggt ggcatacaga
    34621 acttcgtaca gcgttttgcc acgcagttcc ggtttcttcg ccagcagctc ttccggccat
    34681 acttcttcag ttttgaagcg gcgggagaac tggactaact gccagagatc cgatttcgct
    34741 tcgcctggcg cctgtacctg ctgacgccag aactgagtac ggcgttcggc gttaccgtaa
    34801 gcgccctctt tctctaccca cattgcggtc ggcaggatca agtcggcggc cagcgcactg
    34861 actgtcggat acggatcgga gacgatgatg aagttgcgcg gatcgcgcca gcccggcata
    34921 cgctcttcat tgatgttcgg cccggcctgc atgttgttgg tacacatggt ccagtaaaca
    34981 ttcagcttgc cgtctttcag cgcacggtct tgtgccaccg catgaagacc gattttcgcc
    35041 ggaatggtgc cgctcgggat attccacttc ttctcacaga tatcacgatg tttctcgtta
    35101 gttaccacca tgtccgcagg cagacggtga gcaaaggtgc ccacttcacg cgcagtacca
    35161 cacgcagaag gttgcccggt cagggagaac ggaccgcaac ccggctggga aattttgccg
    35221 gtcagcaggt gcaggttgta gaccaggttg ttggcccaca caccacgggt atgctggttg
    35281 aagcccatcg tccagtagga gatgactttc ttgttcggat cggcatacag ctgcgccagt
    35341 tgttctaact ggtctttcgg cacgccggtc atttcagcgg ttttttccag cgtatactca
    35401 gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg aggcgtcaga acccggattc
    35461 ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt agccgatgtc cgtcgcccct
    35521 ttgcgcaggt taacgtgctt gctgaagaag tcctgattta tcgcattgtt ttgaatgata
    35581 tagttggcga tgtagttcag gatcaccagg tcagattgcg gcgtaaagat gatgccgtta
    35641 tccgccagct cgaagctacg atgctggtag gtagaaagca ccgccacggt gacgttctgg
    35701 ttagagagac gacggttagt gatgcgtgac cagaggatcg ggtgcatctc cgccatgtta
    35761 gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt catagcagcc catcggctca
    35821 tccataccaa aggtacgcat aaagccaact actgccgacg ccatacagtg acgtgcgttc
    35881 gggtcgatgt tgttcgaacg gaagcccgct ttgaacagct tggacgcggc ataaccttcc
    35941 cagatagtcc actgaccaga accgaacata ccgatcgatt ccggcccttt ttctttcatg
    36001 gcggttttga atttctcttc catcacatcg aaggcctgat cccaggtgat tggggtaaat
    36061 tcgccttctt tgtcatattt accgtttttc atacgcagca gcggctgcgt caaacggtct
    36121 ttaccgtaca tgatcttggg caggaaatag cccttaatgc agttcaggcc acggttaacc
    36181 ggtgcgtccg ggtcgccctg acaggccacc acacgcccct gctgcgttcc gaccagaacg
    36241 ccgcaaccag taccgcagaa acggcacggc gctttatccc atttaatggc ttcctgctga
    36301 ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg cagccgccgc agcggccgca
    36361 acggcgttag ctttcataaa gctacgacga ctgagtttca tggtgtttcc tcaccttgct
    36421 cttcctgctg gtgataaacc agcgacaccg ccagcacgcc ctctacgttg cgtactgact
    36481 caatggtttg gatcagcgtt tcgctgtctt ctgcttccac caccacaatc aactgaccgc
    36541 tcggcgcgtc gctgacagca acttcacagc cgggaaaggc gttcagttgg gtgctgatgt
    36601 ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca aacttgccag ttagtgtgca
    36661 tggagatact ccgcagttat ggctgatacc ggacagctgg ctgcacatgc tccacatccg
    36721 ttgcaaagtt ggctattaag ttgcgcctgg tagatcccgg agagcgtcgg gcgaaagata
    36781 attgccattg gctcacagct atcctgacag cggcggcatt caactgactg atacgccagg
    36841 cacgcgtccc cgatggtgaa ctgcaaatcc cagaccctgg tgtggcgcgg agaaaatagc
    36901 gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc actcgttatt tttgaaatta
    36961 acgctcggat agccacccgt gccgcgttgc agaatgttgt tttcacaagc attaatacag
    37021 gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat caccggacca gggcggacgg
    37081 ataccgttac tggctttgcg ccagcgacca gtgagtatgc cccgacggga tgcatcaatc
    37141 ttcacattga ccttccatca ttaacgcgct ctgaaaattg agagcaacca aatagaccgc
    37201 ataattaata agccattttt atagccgcta agatattaaa ggatgtgtca aagatgcata
    37261 ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg gcgggaagcg tggcaaaaac
    37321 gggcttttgc tcacatttca aatggttata aacatattta tatagcgatt aattcaccag
    37381 agatatttct gcttgtttgc tccctcatca gaatttaaca ctaaaagagc aggtaaaatt
    37441 gtctgaatgt tctttaagtt attcataaag caaattaata aatctgatga atatgttaac
    37501 cttcagcgac atcatcggtg aaaacctata aatgaagaag gaaagcaaaa aaatgaagac
    37561 cattctacct gcagtattgt ttgccgcttt cgctaccact tccgcctggg cggcagaaag
    37621 cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct gaaacaggca tgaagcgtca
    37681 ggtgattcag ttaaccccgc aagaagatga atctaccctg aaagtagaac tgttaatcgg
    37741 tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc gggaagctgg aaagcaaaac
    37801 gctggaaggc tggggctatg attattatgt ctttgataaa gttagctcgc cggtttccac
    37861 gatgatggcc tgcccggatg gcaagaaaga gaagaaattt gtcaccgcgt atctgggcga
    37921 tgctggaatg ctgcgttaca acagcaagct gccgatcgtg gtgtatacgc cagacaatgt
    37981 agatgtgaaa taccgcgtct ggaaggcgga agagaaaatt gacaacgcgg aagttcgcta
    38041 aactgttgtg aagtacggca ccccgttggt tggataaggc ggttacgccg catccaacat
    38101 ctaacgcccg agccggttgc ctgatgcgac gctggcgcgt cttatcagta tacgccacat
    38161 ccggcatacc atgccggatg tggcacatca ttacaacgca atatccgcca cttctttttg
    38221 aacgggttgc ggtttcaact gcggtttcgg cgtactatct gctgcttgcg gtttgtcgta
    38281 tttcagcccc agcacttcgc tggtgtactg caactcgcgt tctgttgccg ctacatcacc
    38341 gttcagcttg cgtccataag acggaacgat cgctttcaac gtagactgcc attgcgggct
    38401 ggaaacgcga tcgccaaata ctttttccag cagattcaac ataatcggcg cggcggttga
    38461 cgcccctggc gatgccccca gcagcgccgc aatggtccct tgctggtcac tgaccacttc
    38521 ggtacccaga cgcagtacgc cacctttctc ggcatcacgc ttgataatct gcacacgctg
    38581 ccccgcttgc cacaaacgcc agtcctcttt tttcgcttgc ggatagtact ctttcaacgc
    38641 ttcaaaacga tcctcttcac tcaacatcac ctgactcacc agatatttca ccagatcgaa
    38701 attatccagc ccgacgtgca tcatcggcat cacgttagag gtggtggtgg aactcatcag
    38761 atcccacaat gaaccgtttt tgaggaattt ggttgagaag gtggcaaatg gcccaaacag
    38821 cactacgcgt ttaccgtcca gaacacgggt atcgatatgc ggaaccgaca tcggtggtgc
    38881 gccaacggat gctttaccgt aaactttcgc cagatggcga ttaaccacgt ctggattttc
    38941 cgaaacaagg aactgtccgc ccaccgggaa accggcgtag tctttcgctt ccggaatccc
    39001 cgattcctgt aacagcttca gcgccgcacc gcccgcgccg ataaagacaa atttggtgcg
    39061 gatgttctgt gcagtgccgt ttttcagatc ggcaacggta acggtccagg tgttatcgtc
    39121 attacgcttt agggcgcgga cttcgctgct gagttgcagc gagaagttag atttcttctg
    39181 taaggaagca attaactggc gggtgatctc accatagttc acatcagtgc caatttctgt
    39241 tgcggatcgc gcccttccat cactaacggt gcccactctt tgatctgcgc gtgatcttca
    39301 gagtaacgca taccgcgaaa cagcgagctt tgttgcaacg cggcgtaacg ggcgcgcagg
    39361 aaattaacgt tatcctcgcc ccagacaaag ctcatatgcg gaacggtatt gataaatgaa
    39421 cgcggagtac gcagcacgcc gcgctcgacc tggtgcgccc agaactggcg ggaaatctga
    39481 aatgcttcgt taatggcgac tgctttttca atactgatgc tgccatcggc gttttgcggg
    39541 gtgtagttca gttccatcag tgcagaatgc ccggttccgg cgttgttcca gccgttcgaa
    39601 ctctcctgcg cgcccccctc caggcgctcc accatggtca tcgaccattc aggctccagc
    39661 tcgcgtaaat aggtccccaa cgtggcgctc ataatgccgc cgccaatcaa cagtacatca
    39721 gtttcctgct cctcggacgc tttcgctttt gccgccatcg aaacggcatt aagccccacg
    39781 gccatcgaga agagcatggc agtcactttt ttcatcttgt taatgcctta cttttagtcg
    39841 ctttattgca ggtgagattt gcgcggcatc aacggtaaca cttaagtaac aacatttaaa
    39901 taatgtttat aaattatatt ctaattttag aaacattata atttcgttga ttaattatag
    39961 ggttattaga aatgaaggga tttttagcat attgacctga cggcagcagt ccgccaggtc
    40021 agcgtcgtaa tattatgccg tccgggcaac ggcatcacgc gaagcggcat cgcgctcttc
    40081 gcccgtcagc tcgctaagtt gcccattgcg catttccagc aggcggtcgg cgtggataaa
    40141 gtaatgatca tcatgactga tagcgaaaat agttttaccc atctcctgca tcagcggcag
    40201 caacacctga taaaactcac gacggaagtg tggatcctga tccgccgccc attcatccag
    40261 caggataata tcgcgttctt ctgccagcgc cagcaacagc ggcacgcgtt ttttctgccc
    40321 ttttgataac ttcaggttaa caatacgccc gttgcttaac tcaagcttat gagccatttt
    40381 cagctgcgcc agccacttct caaccagttg cgggttagcg ggttgcccct ccggccccag
    40441 cagttgatca aacagccaga catcggtaaa cactgccgaa aacagtttgc gataatcttc
    40501 cggttgttcg ccgctgacag gtttgccatc cagcaagatt tcgccgcttt gtggctgata
    40561 caagcccgtc aacaacatcg ccagcgtcga ttttccgcta ccgttgccgc caatcagaaa
    40621 cagcagctcg ccacgtttga tggtgagatt aatcggacca acggaaaacg cgttatcctg
    40681 ataagcaaac gtcacgttac gcagctccag cgtttgccag ttgggaaacg cctgcgggcg
    40741 cggaaactca gctttgaaag gcgcgagcgc gaatttgttc agcttgttaa acgccacctg
    40801 cgccgtcagc agcgtcggca atgcgccaac cgccgaaagc agcggcgtac gcaggaataa
    40861 cagcgtcaac gaataggtcg cagcaacgtt ggtatcagcc caaccgagac tgttcgccat
    40921 ccagaacacc aggccgatag cgcccagcat catgatgttt gaccagttca cggcactaag
    40981 atggaaggtg tctgcgcgaa taatatgatg gcgatactct tgcgcatcgg ggatgtagag
    41041 gttgttaaac acatactcgg cgcgttcccg gttcagagtc agctctttgc gcccttcaag
    41101 tacagtttga aaatccgtgt acagcttgtc ttcggtttca cgtagggtcg ccatatgttt
    41161 gtacacccgc gccaccagca caaaaccgcc ccagatggtg atcgccatcc agatagccgt
    41221 taccagcaac attttgcccg acagcatcca cagatacgcc gatgaaccga tagtgagaat
    41281 gatcccctgc cccagttccg gcagacgcac aaaagcaatg gtgatattgc gcacatcgct
    41341 ggttaatccc gccagcaacg aggcgctacc gagttgttca atgcgctcga cgtgggtatc
    41401 cagaatccgc ttgataaatt cactacgcag tcggtaaacg aagtgatgcc ccaaagtggt
    41461 gagcgccagt tgcgatccga gagtgactgc catcaacagc agcaataatc ccagaaactc
    41521 cggtaacacc agcagactgg tatccgccgt ttcgataagg cgctgattga taaaagcaat
    41581 taagccaatg cctaatgccg cactggcgag gcttagcgcc atcacactga taaatggcca
    41641 gcgatactgc cgccagacaa gtacaagaag ttccatgcag aaaacccgga caatgaatta
    41701 cagcccgcag tttaaacatc ttcgcgcgca cagcaataat aattcttatt tttattcttt
    41761 tttacctgcc tgacggaatg tcaggttgta gcggcagtcg gtggtgagtg gatgaaaccc
    41821 cgctttcaac ggttgaatac cgtgataaaa cagccgcgat tcaccgcccc aggccaccaa
    41881 attgaaaaat cgcgggtaag cccagagaaa cagaaacaat tggcgcgcgc agatccggtt
    41941 cgtctttatc ctgatgcagc gacagtttcg cgctaggagc gtagcggttg ataagacaag
    42001 catctggctg gaaatctgga tagcccgccg ccgtagccgc acgttgacat aaatcatgaa
    42061 agctttgtgg catggcgggc cacggtttat ttgtttgcgg atcaatgggc gaatagagat
    42121 aaccttgccg atgggtcgtc cagcccagat gcccacagtt ggtcatcgcc accgacatgg
    42181 tatatccgcc tggagcgacc atctggcgaa acggcgactg gctggcaacg tcattaatat
    42241 cgcggattag ttgctccgca gcattaaaag caaaacgccg taaaattacc gcaccagccg
    42301 ccagtggctc ttgccacggt tcagcatcgg caaacagatc caacattacc tctcctcatt
    42361 ttcagcttcg cggcgcagca gttgcgcttt acgcgacacg ccccagcggt aaccggaaag
    42421 tgtgccatca ccacggacca ctcgatgaca gggtattacg atagccagct tgttggcggc
    42481 acaggcgctg gcaacggccc gtaccgcttt cggtttgccg atggcgttag ccagttgctg
    42541 ataactgacg gtttcaccac aaggtatcgt gcgcagtgcc tgccagactt gttgctgaaa
    42601 agcagtgccg cgaatatcca gcggtaacgt cagcggcgta tcgcgttgat tgaggctggc
    42661 gatcacttca cgcacatgtt gctgaaacgt cagatcggca ggcgcgttgt cggcagcggg
    42721 aaacatctgc tgcaactcgc tgattaatgt cgcgtcatca tcgcccagca atatcgcgca
    42781 aatcccccgc tcgctttctg ccaccaggca acgacccagc tcacaatcag ccagcgcgta
    42841 acgcaccgcc agattttcgc caccgtgacg gaattgttta gccgtcatgc ccagcgtttc
    42901 atcggctttg cggtagtaac tgctgctgtc ggggaatccg gcgttaagaa tagacgtcgt
    42961 cacgctctcc cctttcgcca gcgattcgcg caaacggcga gcgcgccagg cctgttgcca
    43021 ggctttaggc gtcattccgg tagtagcttt aaacaaccga tgtagatgaa atggactcat
    43081 cgccacctgg tcggctaagg cttccagcgt tacaggcgtt tcctgttcca gcagtcgaca
    43141 cgcgtgggtg attttatcca accgatgttg ccggggattg gctttgtctg gctgacaacg
    43201 tttgcagggg cgaaatccgg cggcgagtgc ctcgctggca tttgcgtaga aggagacatt
    43261 ttcccgcaag gcatgtctgg cgcggcaaga cggacggcaa aagatacccg tggtgcgcac
    43321 ggcgaaaacg aattcgccgt cggcatccgg gtcgcgggct aagacagatt gccagcgttg
    43381 atcgtcagtt aagcatgtgg cgtttttcat aatcagctcc ctggttaagg atagccttta
    43441 gtctgcccgg tcaccatcac gcaaaaacca acaatcttgc gctttaattt ttttcgctga
    43501 caaggaagct tttaaactgt ggtgacatcc aggttttaaa gctatcgcct tctttggtga
    43561 tcatataaac cgccagccct tcccggcgaa caacctcttt ggctttctcc ggcccgagta
    43621 ccatcaagcc agtatcccag gcatcggctt ccagcgccgt cggagcaatc accgtcacgg
    43681 ataccagatt gtgttcgatg ggacgcccgg tttgcggatc gataacatgg gaaagacgtt
    43741 tgccgtccag ttcgtaatag ttacggtagc tgccagaagt gctaatccca tgaccgttga
    43801 tatccaccac agcctgaacc gcgttttctt tatcggttgg tttttgaatc gctacccgcc
    43861 acggctggcc ttcaccgttc ataccacggc tgttcagcgc gccgcccacc gacaccagat
    43921 agcgggaaat cccttcctgc tccatcaagc gtgccaggtg atccgccgca taaccttcac
    43981 cgacggtgga gagatcgaca tataaatccg gcaggtcttt ttgcagatat tgctgatgcg
    44041 actgattgat gaccgtcagg tgctgtaagc cggttttggc tttcatcgca tcgatctgtt
    44101 cctggctcgg aatttgaacc ggctgttgtt ccgggccaaa gccccacaga ttcaccagcg
    44161 gcccgacggt aatatccatc gcaccatcgg tccttgcccc aatgcgcagc gaggtggtga
    44221 cgatatcggc catcgcttca cttaccggcc acggcgacaa actttgcgag tcgttaaagc
    44281 gcatcagcgc ggaatctttt ttataggtcg aaagcagctg atcgtcggcg tccagctggg
    44341 tctgaatctt ttctttaagt tcggcactgc gtttggcatc aatgcccggg atgctggcac
    44401 gccagaaggt acccatggtt ttgccttcaa gaacggtaac ttcagtagcg tgggttttgg
    44461 cgggttgtgg tttttgatcg caaccaacaa agaagagcgc ggcaaccagc agtgccacgc
    44521 gggtaaagct tatttccata cgtgattatc ctcatgcgaa cggtcgcaag agtacaccaa
    44581 aaaactgtgt ttgtacgctg aaaacaatga aaaaagggcc cgcaggccct ttgttcgata
    44641 tccatcgaga ttagaactgg taaaccagac ccagagctac gatgttatca gtgttgatgc
    44701 cagcgtcacg agtgaactgg ttgtcgtcca gcaggttgat tttgtagtca acgtaggtgg
    44761 acatgttttt gttgaagtag taggtcgcac caacatcaac atatttcagg atatcttcgt
    44821 cgtcgtagtt acgaccattg atgacaccca ggtttttacc tttagactgc aggtaagcta
    44881 gggacggacg cagaccgaag tcgaactggt actgagcaac agcttcgaag ttctgtgctt
    44941 tgttcgccca acccaagttg cctacgcgag ttgcgttgta ggtctgggtg tactgagcag
    45001 ctaggtagat gttgttcgcg tcgtatttca gaccaccggt gtaggtttca gcgcggtcgc
    45061 cattaccgat gtaacgctca tcataacggt tcagaccgaa gttctggtca tcagtacgtt
    45121 tggagctgga aactgcagca ccgataccga agccttcgta atcataagta atagaaccgc
    45181 caacgccgtc accgttctga cgcagagctt cacgaccgtt gttggtcatg ccttcacctt
    45241 ccgggctacc gtttttgccc tgatactgaa cagcgaagtt cagaccgtca accagaccaa
    45301 agaagtcggt gctacggtag gttgcgaagc cgttaccacg ctgctgcatg aagttgtcag
    45361 aaccgtaagt gtcaccaccg aattctggca gtacgtcggt ccaggaagtt acgtcgtaaa
    45421 ctacgccgta gttacgaccg tagtcgaaag aacctacatc ctggaatttc agacctgcga
    45481 atgccacacg ggtccaggag ttgttttcgt tttcagcgct gttgccctgg atctgatatt
    45541 cccactggcc gtaaccggtc agctggtcag taacctgagt ttcacctttg aagccaagac
    45601 gcatgtaggt ctggtcgcca tctacagact tgtcgtcaga gaaatagtgc aggccgtcta
    45661 ctttaccgta cagatctaat ttgttgccgt ctttgttgta aacttcagca gcgtttgctg
    45721 ctcctgctac cagcagagct gggaccagga gggacagtac tttaactttc atgttattaa
    45781 ccctctgtta tatgccttta tttgcttttt tatgccactg catactgatt aaccctcatt
    45841 aatcagtcgg caagtccatt ctccccaaaa atgcagaata atccaacacg aatatgatac
    45901 taaaactttt aagatgtttc atttatcgct acagatgttt caaaatgtaa atacaaggga
    45961 actttttaag attattgcgg aatggcgaaa taagcaccta acatcaagca ataataattc
    46021 aaggttaaaa tcaataactt attcttaagt atttgacagc actgaatgtc aaaacaaaac
    46081 cttcactcgc aactagaata actcccgcta tcatcattaa ctttatttat taccgtcatt
    46141 catttctgaa tgtctgttta cccctatttc aaccggatgc ctcgcattcg gtttttttac
    46201 ccttctttac acacttttca ttattctgtg ctaccacaga aaaactataa cgcttgttaa
    46261 ctatttcaca aataattaac atcagcataa tttccagcaa tctttgttta tttacaatta
    46321 atttcgttgg gctttttgta ggttatttgt acagcaaaat ggcgcttgta catctatttc
    46381 cccccataca ggatgataaa tatcacggga gaatagagaa tcatcaatca ggtaagagtc
    46441 tggaatttca cactgtaccc tttatactgc cctatcactt cgcgaagttt taacaggtca
    46501 taaacacgaa tgcgtcagaa agagacaacg gccacgaccc gcttttcact cctaccgggg
    46561 agcattaccc gcttcttttt actgttgatc attgtgttac tggtgacgat gggggtaatg
    46621 gtacaaagtg ccgttaacgc ctggctgaaa gataaaagtt accagattgt cgacattacc
    46681 cacgcaatcc aaaagcgcgt cgatacctgg cgttacgtga ccaggcagat ctacgacaac
    46741 attgccgcga cgacctcccc ctcctccggc gaaggtttac aagagacgcg cctgaaacag
    46801 gatgtctact atctggagaa accacgccgc aaaacggaag cgttaatctt tggctctcac
    46861 gacaactcaa cgcttgagat gactcagcgg atgtccactt atctggatac attgtggggt
    46921 gcagaaaatg taccgtggtc gatgtattac ctgaatggtc aggataacag tctggtgctg
    46981 atctcaaccc taccccttaa agatctcacc tccggattta aagaatcgac cgtcagtgac
    47041 attgttgatt cacgtcgtgc agaaatgttg caacaggcca acgccctcga tgaacgcgaa
    47101 agtttttcta acatgcgccg cctggcctgg cagaacggtc attactttac cttgcgtacc
    47161 acgttcaacc agccgggaca tctggcaacg gtcgtggctt ttgatctgcc gattaatgat
    47221 ttgatcccac cgggtatgcc gctggacagt ttccgccttg agccagacgc gacggcaacg
    47281 ggaaacaatg ataatgagaa agaagggacg gatagcgtca gtatccactt taacagtacg
    47341 aagattgaaa tctcctcggc actcaactct accgatatgc gcctggtctg gcaggttcct
    47401 tatggcacct tattgctgga tacgttgcaa aacattctgc tgccactgct gctgaacatc
    47461 ggtttgctgg cgctggcgtt atttggctat accacattcc gccatttctc cagccgcagt
    47521 acagaaagcg tccccagcac ggcggtcaat aacgaattgc gcattttacg ggcaattaat
    47581 gaagagatag tctcactgtt gccgctcggc ctgctggttc acgatcagga atcgaaccgc
    47641 actgtcataa gtaacaaaat tgccgatcat ttgctgccgc atctgaatct gcaaaacatc
    47701 accaccatgg cggaacagca tcaggggatt attcaggcga cgatcaataa cgagctgtat
    47761 gagatccgca tgttccgcag ccaggtcgcg ccgcgcacac aaattctcat tattcgcgat
    47821 caggatcgcg aagtgctggt aaacaagaaa ctcaagcagg cgcagcgtct gtatgagaaa
    47881 aaccagcagg ggcggatgac ctttatgaaa aacattggcg atgcgctgaa agaacccgca
    47941 cagtccctgg cggagagtgc ggctaaactc aacgccccgg aaagcaaaca actggcgaat
    48001 caggcagatg tgctggtgcg gctggtcgaa gaaatacagt tagcgaacat gcttgcggat
    48061 gatagctgga aaagtgagac ggtgctgttc tccgtgcagg atttaattga tgaagttgtg
    48121 ccttcagtgt tgcctgccat caagcgtaaa ggtctgcaac tgctgattaa caatcatctg
    48181 aaagcacacg atatgcgccg cggcgatcgc gatgccttac gacgtatttt gctgctactg
    48241 atgcaatatg ccgtgacctc aacgcaattg ggaaaaatca cccttgaggt tgatcaggat
    48301 gagtcctctg aagaccgcct gacattccgc attctggaca cgggagaagg cgtaagtatt
    48361 catgaaatgg ataatttgca cttcccgttt atcaaccaga cccaaaacga tcgttatgga
    48421 aaggcggacc cgctggcatt ctggctgagc gatcaactgg cacgtaaact gggcggtcat
    48481 ttaaacatca aaacgcggga tgggcttggt acacgctact ctgtgcatat caaaatgctc
    48541 gccgctgacc cggaagttga agaggaagaa gagcgtttac tggatgatgt ctgcgtaatg
    48601 gtggatgtta cttcggcaga aattcggaat attgtcactc gcctgttaga aaattggggt
    48661 gcaacctgta tcacacccga tgaaagatta attagtcaag attatgatat ctttttaacg
    48721 gataatccgt ctaatcttac tgcctctggc ttgcttttaa gcgatgatga gtctggcgta
    48781 cgagaaattg ggcctggtca attgtgcgtc aacttcaata tgagcaacgc tatgcaggaa
    48841 gcggtcttac aattaattga agtgcaactg gcgcaggaag aggtgacaga atcgcctctg
    48901 ggcggagatg aaaatgcgca actccatgcc agcggctatt atgcgctctt tgtagacaca
    48961 gtaccggatg atgttaagag gctgtatact gaagcagcaa ccagtgactt tgctgcgtta
    49021 gcccaaacgg ctcatcgtct taaaggcgta tttgccatgc taaatctggt acccggcaag
    49081 cagttatgtg aaacgctgga acatctgatt cgtgagaagg atgttccaag aatagaaaaa
    49141 tacatcagcg acattgacag ttatgtcaag agcttgctgt agcaaggtag cctattacat
    49201 gaacaatatg aacgtaatta ttgccgatga ccatccgata gtcttgttcg gtattcgcaa
    49261 atcacttgag caaattgagt gggtgaatgt tgtcggcgaa tttgaagact ctacagcact
    49321 gatcaacaac ctgccgaaac tggatgcgca tgtgttgatt accgatctct ccatgcctgg
    49381 cgataagtac ggcgatggca ttaccttaat caagtacatc aagcgccatt tcccaagcct
    49441 gtcgatcatt gttctgacta tgaacaacaa cccggcgatt cttagtgcgg tattggatct
    49501 ggatatcgaa gggatcgtgc tgaaacaagg tgcaccgacc gatctgccga aagctctcgc
    49561 cgcgctgcag aaagggaaga aatttacccc ggaaagcgtt tctcgcctgt tggaaaaaat
    49621 cagtgctggt ggttacggtg acaagcgtct ctcgccaaaa gagagtgaag ttctgcgcct
    49681 gtttgcggaa ggcctcctag tgaccgagat cgctaaaaag ctgaaccgca gtattaaaac
    49741 catcagtagc cagaagaaat ctgcgatgat gaagctgggc gtcgagaacg atatcgccct
    49801 gctgaattat ctctcttctg tgaccttaag tccggcagat aaagactaat catctgtagg
    49861 ccagataaga cgcgttagtg tcttatctgg catttgcacc gattgccgga tgcggcgtaa
    49921 acgccttatc cggcctacga ttcccattat ttcaacagat tacattaaag taggccagat
    49981 aagacgcgtc agtgtcttat ctggcatttg cactgaatgc cggatgcggc gtaaacgcct
    50041 tatccgtcct acgaatcccg cgatttcctg accctctcgg catataccgt cagcgtcttt
    50101 tttatcacat ccagcgttac cggcttcgag aggcagctgt ccataccgga ctccagacac
    50161 cgctgcttct cttcagccaa cgcattagca gttactccga ttaccggcaa cgtcagtccc
    50221 aactgacgaa tgcgttgcgt caagcggtaa ccatccatat ttggcatgtt gacgtcgcta
    50281 agcacgatat caatatgatt cttactaagt acattaagcg catcgacgcc atcattcgcg
    50341 gttttacatt gatagcccaa cgatcccaac tgatctgcca gcagacgtcg gttaatcgga
    50401 tgatcatcca cgaccagaat catcatatcg tcgttatcgc tgaccgcttt gtccgtcgac
    50461 ggcagagcgt tagcaggatc gtcgctctcc atctcgatca aataaatacg cgccaacaat
    50521 gccggtagct catgcggagc agccacactg tgtacccact cccctggcgc tttctccagc
    50581 ggaataccaa tatggcgacg acagaaggtc actaccgctc tgccctgcca ttttttactc
    50641 actacctcgt cagtgattaa cacatcttcg ggagtcggtt cctgcccttc gtatgttgta
    50701 acgacgatgc cgctgcgctg caaactggtt tccaggaact gacagagcga cgcattgcgg
    50761 accgccagcc agcagcgttt accactcaac ccttccacgc cttttttctg cgggtactga
    50821 gcgccgtaca acggaatacg cacggtaaac tggctgccca ttcccggttc tgaatctacc
    50881 gagatatcgc cgtccatcat gctgatcagt ttttcacaaa tcgccagacc cagaccggtc
    50941 ccctggaagt ttcgctgtac gcccgttcca acctggaaga agggatcaaa caagcgcacc
    51001 acttctttcg ccggaatccc cacgccggta tcgcgaacac ggatagagag ataatcgcca
    51061 tcagcgcgaa catgcaaaac tatacagccg gtatcggtga attttatggc gttactcaac
    51121 aggttggaga tgacctgctg taaacgcatc gggtcgccat ttaaggccac tggcacatcc
    51181 ggttcaataa agcagtacaa gcctaactgc ttgcgtacca ccagcggtaa atagttggcg
    51241 gtgatgtggt tcatcacttc acgcggtgaa aactcacgcg gttcgatctt caactgttcc
    51301 gattcaatct tcgagaaatc gagaatatcg ctgataattt tcaacaacag gctggaagag
    51361 ttgttcattg ccgtcaccag acgatcgacg cctttcggta actctttggt ttgcaacaga
    51421 tccaggttac cgataatgcc atacagcggc gttcgcagct catgactgac ggtggcaagg
    51481 aacatcgatt ttgactggct cgcctgttcc gctgcttgtg ccatctcctg caacgactct
    51541 tccatcttca cgcgcgaaga aacatccacc agcacacaaa tggccacgtt ttcattacga
    51601 tagcgcgaat ggacgaagct gatttgcaga ttggtattgt tgctggtcag gacatcaaca
    51661 aaattgacct gctgcccaca gataatttgc gtcagtcgtt ggcggtcctc atgcgtaagc
    51721 atattgagat aggtatgcgc cagttcgtta cttaaaatat tgacgccatc agcggtacgc
    51781 aaaatgcaga tacccactgg cgcggaggcg acaatcttgc gattgaactg ctcatgttct
    51841 tccagtcgca gggcgtcgct ttccgccgga atgaaaatac gtcgctcgta catccgcgcg
    51901 agagtaaaca atgcagctcc ggcaagcaca ttcagcaaaa ttgcgttaag gatcaacatg
    51961 cgaatgcgtt ccagcacctt atcaaccggc accgaataca cgatgcttag cgatgagggt
    52021 ggcagatttt tcttcagcac cagctcccgg aacccttccg tatagccaaa ccaggagcgt
    52081 tcctgcatcc agcgaggatc gcccttaatt ttactttctg gtccggtaag cgaaatcagg
    52141 gtatgaccat tttcatcaag aatggtaacc cccatcggca acgtacccgg taagaaaaag
    52201 ttctccatcc ggatggtctg ctcgacaccc aaaagcgcct gcaaccggtt cgccagataa
    52261 actggcgtca acgcgtaaaa atacccgacg ccagggcgcg gaccttcgct gatccagtag
    52321 aggttactgc cgctatcatc ttgtggtgca tttcgatatt tattgatgcg ttcatgcaac
    52381 gctttcaacg cggtatcgcg ttccactggc atatcacgca gaccgaaatt ggccatgcag
    52441 aggttatcac tgccgattaa aaatacccgg ttgagatcgt aagccgcaga aaaattatcg
    52501 cgccagtagc gcataaacca cgccaatgac tccagagaac ctcgccaggt gttactcatt
    52561 gcggaacaat cggagtcggc aaacagcggt tcaaacgcag gcacatccgc ctgcgtttct
    52621 cgtccacgcg gggaaagcac accgttttct gccgataagc gattttcggc gatgtactta
    52681 agctctttca tcacatcaga ggtgcgttga ataaagcgct gagcctgatc ggaactcaga
    52741 ttaaattcct gacgaatttc cgattctcgc tgatgtaacg cattaacgat gtaaaaaacg
    52801 gatgaaaaag caatcaacag ccagagcact aacgccaatg ctctgaacat gtagcgcgag
    52861 gctttcaggg ttgtacgaaa agaagcaagg tatttcaaag gggcgaagct ccgcctcagg
    52921 tgaccgatgg agtgtggtta aggtagcggt aaaagcgtgt taccgcaatg ttctgtcacc
    52981 attgcaataa tgcgtatcgc ttagaaacca ttgccttaac caggaaagcc ggagtagatg
    53041 gacagttctc agtacgaaaa ataagaaatg accaatctgc cgacgcttgt taatggttat
    53101 atcctggcgt cggcagatcg cccagcagtc ttatctgaaa aaagagagag ccaggttgtg
    53161 tgaagggatg gatcaccagt caaaactttt caccagcgtc agctcgccag cattacgcat
    53221 cggtacaata aatgtttcct gtttctcatt gaccgatcct tcatcggtga tcagcgtcag
    53281 ttgggcggtc gtcaattccg tttcgctgcg cccaccatag tagttgatat acacctgata
    53341 gcgcccgtga actggtgctg gcatggcgaa aatctcgggt ccgtaccccg tcgtgacatc
    53401 catatccagt gcaccactgt ttttcagcac ggtgttaccg taccaggcgt gttcgccatc
    53461 gggcgtgaca acatggagat cgagatcggt attgtccata tcccacgaga gaaccagccg
    53521 taaacgtgca cgaatagttc ctgtacccgg cgttgagtaa aactgcattt tttgccggct
    53581 ttgcccatcc gggctgatga cctgcacgct gttgctgcct tcagtgaaaa tataagggcg
    53641 tgcaaatgaa ccatcggatt caattctctg cggcatactg gcaccgttga ccaccagccg
    53701 gccctgctga actttacccg ccgcagcata attctttatt ttgccgctga tctgcgctga
    53761 gatattttga tcatctgcca tgttgacgga cgacgccggg taattgatcg attgtgaaaa
    53821 actggcatct tcgccctctg ccggatgcca gccagagagc ggtgcgtcaa tctcgacttc
    53881 ctgcacacct tcactgtgag cgacaggcga aagtgccacc agtaacaacg gaagaaaaat
    53941 ctttcgcata gcattagtcc attaataatt gtcgggtcag cgtttcgata tagttctcat
    54001 caagcccggt gggataacca tcaaaggcca agtgcagata ctcatgggtt aaatccagcc
    54061 gatcctgaag cgtaaagaag ttacgaataa acagccgttt ctgctgccga tcggtatagg
    54121 ggaaacctga gactaaacga cagaccgcaa acacgtctgg ttcgttgtac cccgtctcgc
    54181 cttgtaatat acgccgccac tgcggcattt ttttcgccag ccaggctttc gctttgggta
    54241 ataactggca ggttgagcgt ggagctcccc agcggctaag gctgttgtcg ggataagcaa
    54301 acgcgagtat ctggtcgtaa cgctccccct gccccgcctg ggccgtggct tgccgccagg
    54361 aaagtgtccc ttcggtagcg cgactgccat gataatgaac cggatcgcct gcatagatga
    54421 gctcctgcgt ccaggcggtc atcgttcgcg cccccgtggt cgctggcgaa gcagaaacac
    54481 gctgcgtggc gctgctgccg gggatggtca ggcaatcacc ttcccgattg gcgttttgct
    54541 gtaaaaaagt acgtatcgcc acagtcatgg ctttcgctgc ctcaggtggc gtacttttcg
    54601 cctcgcgatc cagaactctg gcaacgtact cttcacgatc gagatgcgac tgcaatttca
    54661 gtttgccctt ttcgctcagt aaggtcgtct caccgtggct gacaaacgta atgtggttac
    54721 cattagtaaa cgtcacacga tagcgcccat taagtacgct gggttttact gccgtcgtac
    54781 tcttttccgc tgtgattttc ttcagcggat agcgggcaaa cagctccacc tctacacatt
    54841 gcccactggc aacctgcgtc ggcactggca aaacccgatt aagcaccgtc gcataacggg
    54901 ttagtaccgt tttgctggtg ccactgccag taacccacag cggtgtacca tcggttaacc
    54961 agccagcaaa accaccctga cggatttcct gtttatcatc ggcaaaccag ctccaggttt
    55021 tcacccgtac ccgactaccg agcatggatg agacaccgat tttcgcctcg tccagcacca
    55081 catccagtaa gacttcctga gctttgtttt gcgcaggtaa cgtcgctaat gaatcaagca
    55141 gcgatttaac cgttaccgag gtttctggct tcagcgttgt aagagacgct aaccacgcag
    55201 gtgcctgacg ctgttgccag tattgccccc acacatctgc gccaatatgc aaccgctgcg
    55261 gcgcaaaata gagcccacag gaacgtacca gcgcggtatc gcgcgttatg ctctccccag
    55321 cctgacaaca ataaacctct tccggggagt ttccgcgaca ctggtagggc tgctccggtt
    55381 gatgggtatc ctccagccag gcgtaaacat acagtttcca caaactccct aacggcgtgg
    55441 tgagcgagtc aggtaatgct gagactttgg tcacgcctga tgacgacagc tgatacagtt
    55501 gatcgtgctg cgcaccgcgc agcaccagtt gtaacggcgt ctcctccgca agcgttggca
    55561 gagtaaccaa cgccaatagc cagacgattc tgcgccagtt cacttatttc acctgcatcc
    55621 cggtccattc actccctgcc gcgacacttt gctgcgcagg tgcataggaa cgcacataac
    55681 gagcaggagg cagaacgaat tgcccttttt gcgagaagcg cagcaaatgg cggaaagtga
    55741 ccgttcccgt cagttctttc accggcacca tataggccag ttcgcccatt tcattacgcg
    55801 ctttttccag caattgcccc tgctgtttcg cggcgttggg tttattgact gaaatgcccc
    55861 atgttgtgcg ctcaacgtcg gctcccggcg ggagcggtac ttccacctga ccatagcgca
    55921 gaactgcatc ctgctcgcag gtaagcgtaa tttcatcgag atacagcgca tcgctgtcaa
    55981 tctcattgct ggtcaccggc tgcagagtaa agctcatctc ttcttcaccg gggataagcc
    56041 gatacaactg gcgttcaacg gtcaccggaa tgttactttg ttgagccgtt tttgccggtt
    56101 cacgccagcg gacctgcaca ttttgcggcg ataattcgtc accaaaagag agaatgtccg
    56161 gcacaccctg accaacccaa cgccagtctt cgccccctcc agttaactta tgtttagccc
    56221 atgcgcccgc aggcgcaggc aaaacaactg gaggcattgt cgccatatat ttcgccagcc
    56281 agttcatggc cagcgcgcgc tcaatagtgg attgctcagc ggtcaaacca ttaaaatagc
    56341 ggccgtctgg gtagcgtcac caccgctatt gagcagcaca accgtgtgcg ccagcggctg
    56401 attgctgttt gccgctttat cccacgcata ttgctgaacc tgtggcatca ccgtggactt
    56461 caactgcgct tttttcagca acgtcatggt taagacgttt gccaccgcat ctgccagtgg
    56521 agattccggt gtatcgagga tcaggctgtc attgatatcg cggatgtctt cctcagagaa
    56581 atcttcatct ttagttccgc gccgggcgat agcttcatcc ccccctttca acaacgtttt
    56641 gcacggcaga ttcatctcct gtgcccacgc cagcaccagc gcccgatgca ataacggcat
    56701 gttatccgcc tgctcggcat aactgtcgag catatgctgc cagtattccg gttgttgcgt
    56761 tacgccgagc gcctggctgg cctgccaatc ggcgtaccat gcccatgccg taaggaaggc
    56821 gtcaccattg ccatcttcac cccaccaggt aaagcgcgct ccgggccccg ccagttgcat
    56881 cagccgcaga cggttatcct gaatcatctg acgaatgtcg ttagcggcgg cactttgatg
    56941 atcggcaagc gaacgccagg cgagactgag cgggatcaga cggctaccgg tgttgatcac
    57001 cccaccccac ggttcatcga ctaacgcatc aagattgttg cggaaaatct cctgcggcgt
    57061 ttcactactt tgtagccgga tattactcgc ctgctcgggc aacatcagcg cgttctcgcc
    57121 accgccgagc atgacatttt tctgctgttc aacgggccag ctgttgtcca caaaagacag
    57181 ttttgtgcta atgctgtcct gcacttgccc attttgttgc agttctgcac ttaacaagcc
    57241 agattgctga atattctgcg tcagcgaaat ataattcgcc cctttgtgca gcgtcagcgt
    57301 ctggcgcatc tcagcgcctg caaatttagt caccagcgcc accggttcgt tatcctgctg
    57361 actgaagata aacagtcctg ccgccggttt gtcgcccacg cgatacaccg ttggcatact
    57421 ccacttcatg tagagatttt tttccgaacg cagataagca cgcccctgcc cgaccagccc
    57481 gtcgccgttc atcccacgcg cggtgatacg ccagcgggtt aacgaatcag gcatcaggaa
    57541 cgtgaaatac gctttgcctt gtttatcggt tgtgagtgac ggcatccatg ccgcggtatc
    57601 cacctcttca cgccgtggac gttcaggcat ttttactcgc cgctcgctgc ggttagtcgc
    57661 gccaggcgca accggctcgc tggagagcgc ctggtcatag ctgataaacg acagactgga
    57721 gctggtacgc acattgttac gccccagcgg atagaaaaat ttgccgatat tcggcgcgat
    57781 ttctggttgc agcgcgtaga tcatttcatc gaccacgcct accgttagct gcgcagaaac
    57841 aggtttacct ttcagcgacg aggttaat
//
LOCUS       DAJTRQ010000012        56575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000012 DAJTRQ010000000
VERSION     DAJTRQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..1017)
                     /gene="setA"
                     /locus_tag="OO332_000838"
     CDS             complement(<1..1017)
                     /gene="setA"
                     /locus_tag="OO332_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5941416.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGA"
     gene            complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="OO332_000839"
     CDS             complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="OO332_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5941417.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1339..2994
                     /gene="sgrR"
                     /locus_tag="OO332_000840"
     CDS             1339..2994
                     /gene="sgrR"
                     /locus_tag="OO332_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5941418.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3028..3127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3158..4141
                     /gene="thiB"
                     /locus_tag="OO332_000841"
     CDS             3158..4141
                     /gene="thiB"
                     /locus_tag="OO332_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5941419.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4117..5727
                     /gene="thiP"
                     /locus_tag="OO332_000842"
     CDS             4117..5727
                     /gene="thiP"
                     /locus_tag="OO332_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5941420.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5711..6409
                     /gene="thiQ"
                     /locus_tag="OO332_000843"
     CDS             5711..6409
                     /gene="thiQ"
                     /locus_tag="OO332_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5941421.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6523..7287)
                     /locus_tag="OO332_000844"
     CDS             complement(6523..7287)
                     /locus_tag="OO332_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5941422.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7373..8251)
                     /gene="araC"
                     /locus_tag="OO332_000845"
     CDS             complement(7373..8251)
                     /gene="araC"
                     /locus_tag="OO332_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5941423.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8590..10226
                     /gene="araB"
                     /locus_tag="OO332_000846"
                     /pseudo
     CDS             8590..10226
                     /gene="araB"
                     /locus_tag="OO332_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10237..11739
                     /gene="araA"
                     /locus_tag="OO332_000847"
     CDS             10237..11739
                     /gene="araA"
                     /locus_tag="OO332_000847"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5941424.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11939..12634
                     /gene="araD"
                     /locus_tag="OO332_000848"
     CDS             11939..12634
                     /gene="araD"
                     /locus_tag="OO332_000848"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5941425.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12709..15060
                     /gene="polB"
                     /locus_tag="OO332_000849"
     CDS             12709..15060
                     /gene="polB"
                     /locus_tag="OO332_000849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5941426.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15224..18130
                     /gene="rapA"
                     /locus_tag="OO332_000850"
     CDS             15224..18130
                     /gene="rapA"
                     /locus_tag="OO332_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5941427.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18142..18801
                     /gene="rluA"
                     /locus_tag="OO332_000851"
     CDS             18142..18801
                     /gene="rluA"
                     /locus_tag="OO332_000851"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5941428.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="OO332_000852"
     CDS             complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="OO332_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5941429.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19988..22342
                     /gene="lptD"
                     /locus_tag="OO332_000853"
     CDS             19988..22342
                     /gene="lptD"
                     /locus_tag="OO332_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5941430.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22395..23681
                     /gene="surA"
                     /locus_tag="OO332_000854"
     CDS             22395..23681
                     /gene="surA"
                     /locus_tag="OO332_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5941431.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23681..24670
                     /gene="pdxA"
                     /locus_tag="OO332_000855"
     CDS             23681..24670
                     /gene="pdxA"
                     /locus_tag="OO332_000855"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5941432.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24667..25488
                     /gene="rsmA"
                     /locus_tag="OO332_000856"
     CDS             24667..25488
                     /gene="rsmA"
                     /locus_tag="OO332_000856"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5941433.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25491..25868
                     /gene="apaG"
                     /locus_tag="OO332_000857"
     CDS             25491..25868
                     /gene="apaG"
                     /locus_tag="OO332_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5941434.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25875..26717
                     /gene="apaH"
                     /locus_tag="OO332_000858"
     CDS             25875..26717
                     /gene="apaH"
                     /locus_tag="OO332_000858"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5941435.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26795..27274)
                     /gene="folA"
                     /locus_tag="OO332_000859"
     CDS             complement(26795..27274)
                     /gene="folA"
                     /locus_tag="OO332_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5941436.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="OO332_000860"
     CDS             complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="OO332_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5941437.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="OO332_000861"
     CDS             complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="OO332_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5941438.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29898..31292)
                     /locus_tag="OO332_000862"
     CDS             complement(29898..31292)
                     /locus_tag="OO332_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941439.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="OO332_000863"
     CDS             complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="OO332_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5941440.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31635..32921)
                     /locus_tag="OO332_000864"
     CDS             complement(31635..32921)
                     /locus_tag="OO332_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5941441.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32972..33913)
                     /locus_tag="OO332_000865"
     CDS             complement(32972..33913)
                     /locus_tag="OO332_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5941442.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33928..34698)
                     /locus_tag="OO332_000866"
     CDS             complement(33928..34698)
                     /locus_tag="OO332_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5941443.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="OO332_000867"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="OO332_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5941444.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="OO332_000868"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="OO332_000868"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5941445.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39202
                     /gene="caiB"
                     /locus_tag="OO332_000869"
     CDS             37985..39202
                     /gene="caiB"
                     /locus_tag="OO332_000869"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5941446.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39276..40829
                     /gene="caiC"
                     /locus_tag="OO332_000870"
     CDS             39276..40829
                     /gene="caiC"
                     /locus_tag="OO332_000870"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5941447.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40938..41723
                     /gene="caiD"
                     /locus_tag="OO332_000871"
     CDS             40938..41723
                     /gene="caiD"
                     /locus_tag="OO332_000871"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5941448.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41729..42319
                     /gene="caiE"
                     /locus_tag="OO332_000872"
     CDS             41729..42319
                     /gene="caiE"
                     /locus_tag="OO332_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5941449.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="OO332_000873"
     CDS             complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="OO332_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5941450.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43062..46283)
                     /gene="carB"
                     /locus_tag="OO332_000874"
     CDS             complement(43062..46283)
                     /gene="carB"
                     /locus_tag="OO332_000874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5941451.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46301..47449)
                     /gene="carA"
                     /locus_tag="OO332_000875"
     CDS             complement(46301..47449)
                     /gene="carA"
                     /locus_tag="OO332_000875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5941452.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="OO332_000876"
     CDS             complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="OO332_000876"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5941453.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="OO332_000877"
     CDS             complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="OO332_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5941454.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="OO332_000878"
                     /gene_synonym="lytB"
     CDS             complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="OO332_000878"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5941455.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="OO332_000879"
     CDS             complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="OO332_000879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5941456.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="OO332_000880"
     CDS             complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="OO332_000880"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5941457.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="OO332_000881"
     CDS             complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="OO332_000881"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5941458.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="OO332_000882"
     CDS             complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="OO332_000882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5941459.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55662..55880)
                     /locus_tag="OO332_000883"
     CDS             complement(55662..55880)
                     /locus_tag="OO332_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5941460.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55983..56246
                     /gene="rpsT"
                     /locus_tag="OO332_000884"
     CDS             55983..56246
                     /gene="rpsT"
                     /locus_tag="OO332_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5941461.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13544 a        15497 c        14681 g        12853 t
ORIGIN      
        1 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
       61 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
      121 aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt
      181 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
      241 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa
      301 cggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa
      361 catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag
      421 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
      481 cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag
      541 cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
      601 cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc
      661 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
      721 aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg
      781 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
      841 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
      901 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
      961 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     1021 ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga
     1081 ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca
     1141 tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac
     1201 cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc
     1261 cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg
     1321 cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg
     1381 caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc
     1441 tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg
     1501 tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg
     1561 gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg
     1621 cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc
     1681 ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta
     1741 cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta
     1801 acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa
     1861 atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt
     1921 gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat
     1981 tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag
     2041 ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa
     2101 tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt
     2161 cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca
     2221 ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat
     2281 ctgatgctaa aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt
     2341 tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac
     2401 tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag
     2461 caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag
     2521 agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag
     2581 catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa
     2641 atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt
     2701 aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg
     2761 ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat
     2821 ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg
     2881 cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat
     2941 accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg
     3001 gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc
     3061 gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc
     3121 ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct
     3181 gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga
     3241 ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg
     3301 taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg
     3361 gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga
     3421 cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt
     3481 tcccagcggc tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt
     3541 ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga
     3601 tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct
     3661 gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa
     3721 gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga
     3781 aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa
     3841 agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc
     3901 ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc
     3961 tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac
     4021 gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc
     4081 tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta
     4141 attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc
     4201 gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag
     4261 gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta
     4321 ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt
     4381 cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt
     4441 ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc
     4501 ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt
     4561 tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa
     4621 caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa
     4681 tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc
     4741 gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg
     4801 gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg
     4861 ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt
     4921 gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc
     4981 atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg
     5041 gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg
     5101 caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg
     5161 accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt
     5221 caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc
     5281 ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg
     5341 attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg
     5401 cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg
     5461 cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt
     5521 gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc
     5581 cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg
     5641 gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta
     5701 ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg
     5761 ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg
     5821 taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac
     5881 tatcgatggc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt
     5941 tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa
     6001 tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat
     6061 ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg
     6121 cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt
     6181 ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca
     6241 gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc
     6301 cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt
     6361 gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc
     6421 agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga
     6481 cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt
     6541 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
     6601 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg
     6661 acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac
     6721 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc
     6781 tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag
     6841 caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc
     6901 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     6961 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     7021 tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc
     7081 cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc
     7141 aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc
     7201 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt
     7261 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     7321 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     7381 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     7441 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     7501 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     7561 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     7621 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     7681 ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga
     7741 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     7801 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     7861 tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg
     7921 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     7981 attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     8041 tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     8101 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     8161 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     8221 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     8281 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     8341 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     8401 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     8461 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     8521 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg
     8581 agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg
     8641 gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg
     8701 caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac
     8761 attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc
     8821 gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc
     8881 gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta
     8941 ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg
     9001 ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg
     9061 gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg
     9121 attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt
     9181 cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg
     9241 ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg
     9301 ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg
     9361 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
     9421 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
     9481 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
     9541 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
     9601 agcatgcgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
     9661 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
     9721 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
     9781 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
     9841 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
     9901 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
     9961 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    10021 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    10081 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    10141 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    10201 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    10261 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    10321 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    10381 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    10441 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    10501 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    10561 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    10621 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    10681 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    10741 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    10801 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    10861 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    10921 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    10981 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    11041 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    11101 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    11161 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    11221 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    11281 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    11341 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    11401 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    11461 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg
    11521 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    11581 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    11641 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    11701 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    11761 cgcctgatgc gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    11821 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    11881 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    11941 gttagaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    12001 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    12061 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    12121 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    12181 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    12241 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    12301 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    12361 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    12421 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    12481 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    12541 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    12601 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    12661 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    12721 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    12781 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    12841 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    12901 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    12961 gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac
    13021 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    13081 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    13141 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    13201 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    13261 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    13321 ctggaaaaac tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac
    13381 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    13441 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    13501 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    13561 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    13621 aaatctatcg ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat
    13681 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    13741 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    13801 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    13861 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    13921 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    13981 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    14041 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    14101 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    14161 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    14221 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    14281 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga
    14341 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    14401 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    14461 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    14521 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    14581 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    14641 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    14701 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    14761 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    14821 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    14881 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    14941 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    15001 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    15061 gcaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
    15121 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
    15181 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
    15241 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
    15301 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
    15361 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
    15421 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
    15481 tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
    15541 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
    15601 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
    15661 cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg
    15721 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
    15781 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
    15841 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    15901 ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    15961 tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct
    16021 ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt
    16081 gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    16141 gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc
    16201 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca
    16261 gaaaaattat cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag
    16321 caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt
    16381 gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    16441 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa
    16501 aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac
    16561 ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga
    16621 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    16681 cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    16741 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg
    16801 tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc
    16861 tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg
    16921 ttctgaagga cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa
    16981 cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat
    17041 tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca
    17101 cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt
    17161 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    17221 gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga
    17281 ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    17341 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    17401 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    17461 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga
    17521 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    17581 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa
    17641 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    17701 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    17761 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    17821 cgcggttaac cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc
    17881 tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    17941 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc
    18001 agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    18061 aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac
    18121 gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg
    18181 ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    18241 tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    18301 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    18361 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    18421 aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg
    18481 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa
    18541 cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg
    18601 ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat
    18661 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg
    18721 ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt
    18781 aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt
    18841 cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca
    18901 ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc
    18961 tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc
    19021 accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc
    19081 gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc
    19141 tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca
    19201 ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca
    19261 gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc
    19321 tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga
    19381 aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt
    19441 tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata
    19501 tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa
    19561 gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg
    19621 ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg
    19681 ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc
    19741 ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt
    19801 tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca
    19861 atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac
    19921 aagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa
    19981 tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta
    20041 tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta
    20101 tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca
    20161 cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg
    20221 taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc
    20281 ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat
    20341 cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg
    20401 tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg
    20461 cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga
    20521 cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat
    20581 ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt
    20641 gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac
    20701 caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac
    20761 catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta
    20821 cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta
    20881 tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc
    20941 cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag
    21001 ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa
    21061 attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca
    21121 ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta
    21181 ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt
    21241 taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt
    21301 gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta
    21361 tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt
    21421 taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga
    21481 aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc
    21541 gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc
    21601 tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt
    21661 gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc
    21721 cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga
    21781 gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga
    21841 gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag
    21901 taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta
    21961 cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata
    22021 taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc
    22081 cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg
    22141 tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg
    22201 ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact
    22261 tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct
    22321 gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt
    22381 gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga
    22441 ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg
    22501 tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg
    22561 caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca
    22621 tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc
    22681 tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca
    22741 gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga
    22801 tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg
    22861 aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc
    22921 tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg
    22981 aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg
    23041 cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta
    23101 ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg
    23161 ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg
    23221 aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc
    23281 cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga
    23341 gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta
    23401 accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg
    23461 ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc
    23521 atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc
    23581 gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg
    23641 aacaacgtgc caacgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt
    23701 tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca
    23761 gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc
    23821 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc
    23881 gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg
    23941 gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg
    24001 ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa
    24061 cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa
    24121 aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt
    24181 accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat
    24241 tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg
    24301 gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat
    24361 tccggtgctc gacgagctgc gggcgcaggg aatgaaactc aacgggccgc tgcctgccga
    24421 taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga
    24481 tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg
    24541 cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg
    24601 cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa
    24661 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac
    24721 tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc
    24781 caggcaatgg tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt
    24841 ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat
    24901 ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc
    24961 gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc
    25021 tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt
    25081 atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt
    25141 cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg
    25201 tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca
    25261 acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt
    25321 aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta
    25381 accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc
    25441 cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt
    25501 cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata
    25561 atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc
    25621 agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg
    25681 gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca
    25741 gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg
    25801 aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca
    25861 ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac
    25921 tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg
    25981 gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag
    26041 gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg
    26101 ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg
    26161 acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc
    26221 tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg
    26281 cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt
    26341 acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta
    26401 tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca
    26461 gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc
    26521 ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta
    26581 cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc
    26641 tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg
    26701 aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt
    26761 cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc
    26821 aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt
    26881 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    26941 gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca
    27001 tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta
    27061 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
    27121 gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    27181 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    27241 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc
    27301 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27361 tttatattct gctggcgagt ccacgctctc cccctggact cgccgcatta caatgaaaca
    27421 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc
    27481 gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca
    27541 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg
    27601 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct
    27661 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27721 cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca
    27781 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg
    27841 cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg
    27901 gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc
    27961 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt
    28021 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28081 cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca
    28141 tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc
    28201 accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28261 cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca
    28321 ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt
    28381 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28441 atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28501 aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat
    28561 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28621 tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc
    28681 cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat
    28741 accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28801 gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata
    28861 tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt
    28921 tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat
    28981 tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa
    29041 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29101 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29161 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29221 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc
    29281 gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg
    29341 ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca
    29401 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29461 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29521 ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc
    29581 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29641 aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc
    29701 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29761 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29821 atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt
    29881 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    29941 aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc
    30001 tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    30061 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc
    30121 ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg
    30181 gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc
    30241 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    30301 tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    30361 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    30421 aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa
    30481 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    30541 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    30601 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc
    30661 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg
    30721 aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa
    30781 ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    30841 cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    30901 atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc
    30961 accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg
    31021 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc
    31081 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg
    31141 tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    31201 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat
    31261 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    31321 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    31381 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    31441 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    31501 acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac
    31561 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    31621 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    31681 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    31741 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    31801 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg
    31861 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    31921 gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga
    31981 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    32041 ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    32101 accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc
    32161 atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc
    32221 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    32281 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    32341 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    32401 tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt
    32461 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    32521 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    32581 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    32641 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    32701 tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga
    32761 ctgtgggcat agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg
    32821 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    32881 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    32941 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    33001 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    33061 ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga
    33121 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    33181 cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca
    33241 actccgcacc tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa
    33301 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    33361 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    33421 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca
    33481 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    33541 gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg
    33601 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca
    33661 gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt
    33721 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    33781 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    33841 gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag
    33901 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    33961 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    34021 cgcggcaacc tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga
    34081 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    34141 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    34201 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    34261 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    34321 ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc
    34381 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    34441 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    34501 ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg
    34561 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    34621 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    34681 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    34741 agtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    34801 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    34861 aaaacatggc gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa
    34921 gtcagatgct attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa
    34981 cgaaaaacgt cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt
    35101 aaatgttaac aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat
    36241 ccacctggat cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag
    36481 tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
    37501 ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag
    37921 ccggataagg cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga
    38101 agttatttgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
    39181 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
    39241 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
    39301 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
    39361 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
    39421 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc
    39481 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
    39541 tggtgccgat taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc
    39601 aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg
    39661 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
    39721 gagtgagttc atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
    39781 cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
    39841 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
    39901 gtgcactgcg tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc
    39961 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
    40021 acagcgcccg cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta
    40081 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
    40141 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg
    40201 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
    40261 tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
    40321 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
    40381 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
    40441 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg
    40501 acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
    40561 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca
    40621 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
    40681 tgaatgaagg tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg
    40741 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
    40801 ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta
    40861 ataatcacgc cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc
    40921 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
    40981 aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
    41041 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
    41101 ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
    41161 cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca
    41221 aaccagttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg
    41281 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg
    41341 gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg
    41401 tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga
    41461 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc
    41521 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
    41581 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
    41641 catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg
    41701 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
    41761 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
    41821 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
    41881 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
    41941 caccatcgtt ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat
    42001 tggtcgcgat gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga
    42061 agagagcatt gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca
    42121 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
    42181 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
    42241 tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
    42301 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
    42361 catcgtatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
    42421 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    42481 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
    42541 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
    42601 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    42661 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    42721 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
    42781 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
    42841 cgttttgaag caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac
    42901 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    42961 aaagatttac ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc
    43021 gatcaaatta gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc
    43081 atttcctgca ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg
    43141 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
    43201 acgcgggagt cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg
    43261 ccattcttga tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc
    43321 ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
    43381 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
    43441 gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg
    43501 aacgcttcag cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc
    43561 cctaacagcg ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag
    43621 tagtacggcg gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
    43681 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta
    43741 cgcgccgcac gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc
    43801 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
    43861 acatcctgaa tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag
    43921 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
    43981 cagatggcat ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc
    44041 gcatcgttag acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
    44101 acgatttcca tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca
    44161 atctcttttg ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt
    44221 ttcagtttca gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata
    44281 gcatccgggc tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc
    44341 agcggggttt gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
    44401 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
    44461 gtagagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc
    44521 gccagcgagg cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga
    44581 ccgccgccaa gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
    44641 tcataagtgg agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg
    44701 cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt
    44761 acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
    44821 aattcagcgt tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt
    44881 tcttcaatct gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca
    44941 gacaggcccg cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc
    45001 agttcgcgac ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag
    45061 ccagttgcgc ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
    45121 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg
    45181 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
    45241 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc
    45301 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
    45361 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
    45421 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
    45481 gaggcgttac gcatgatttg atattctttg tcagtcagcg tttgggctgg cgcgacagtg
    45541 atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
    45601 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
    45661 tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca
    45721 aactcttcac ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
    45781 ataatgcacg ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg
    45841 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
    45901 tcttctgctt tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc
    45961 aacacgccct gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt
    46021 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc
    46081 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
    46141 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
    46201 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
    46261 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
    46321 actgctcaat taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc
    46381 tggcttcagg gtgaccctgg aagctgaacg ccggtttatc gatacgatga atgccctgta
    46441 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
    46501 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
    46561 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
    46621 gcgccagcag ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact
    46681 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
    46741 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
    46801 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
    46861 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
    46921 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
    46981 ccatgccgtt cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt
    47041 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
    47101 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
    47161 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
    47221 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
    47281 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
    47341 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
    47401 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acacctccat
    47461 agaatattca ctcactttat ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa
    47521 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
    47581 caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    47641 agcgacaaaa acatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat
    47701 gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
    47761 aaatagaaga cgaaacatgt aatttttggc agttatagaa aatttaaaag cataacacca
    47821 aaaataaaag ggctaaaaat taaagccctt tataattaat aaattaaatg tgttactttt
    47881 gcatcataat aaacattttg tggttacaaa ttattgagat caagcacatc tcgcatatca
    47941 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcacc gttagcaaat
    48001 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
    48061 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc
    48121 acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
    48181 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
    48241 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
    48301 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
    48361 gcaatatcgg cggcggcgtc acgaattgcc tgtttaccgg cttcgtcaaa ccccgtagtg
    48421 ccgatcacca tccctttgcc atgctgccga caaaaagcga gatggttcag cgtaccttcc
    48481 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta tcgcatcaag actgctttgc
    48541 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
    48601 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag tgccgcctga
    48661 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
    48721 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
    48781 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
    48841 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacggcac
    48901 cagagccagc acctcagcca cccactgttg gaagcctttt acattcagat ccaatgccac
    48961 ctgtacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
    49021 aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg
    49081 caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct
    49141 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggtaaagt
    49201 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacaat
    49261 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
    49321 ggcatttggt gtacagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
    49381 atacggttta cattccgggc attgtaaaag taacagcgca atattggtta acgggccgat
    49441 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
    49501 cggtatcccg agtggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
    49561 gccgtgcaca gatgctgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
    49621 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc
    49681 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat
    49741 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
    49801 acgcatgttt tcttcataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
    49861 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
    49921 acaatatttt cttcacggcc ttccagcgga atggcttcac caccacccaa ctgctgcaaa
    49981 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
    50041 catttaacct ctttcaccca ctcttcctgg atatctttcg catcgtcaat caaaaacgcg
    50101 tgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
    50161 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
    50221 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
    50281 agcgcgtcga tcacatcaga cgtatcatcc accgacagcg tggtttgggt cataaaggag
    50341 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ctggcgattc gaccagatac
    50401 attccccctt cggggttgct gtactgcccc atcgtccctt ccacttccgg gtgcccggcg
    50461 tggccgatga gaatagattc ttcgccacgg cgactggcgc gggcaacttc catatgcact
    50521 ttggtcacca gcggacaggt ggcatcaaac accgtcaaat cgcggctttt cgcttcgtta
    50581 cgaaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggcacttcg
    50641 ctaatctgct caataaagat agccccacgc tcgcgcaagc tatcgaccac gtagcggtta
    50701 tgcaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
    50761 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
    50821 atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
    50881 cccggccagc ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
    50941 accaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
    51001 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
    51061 aaacgccgca tctggctcca gcgagaaggt ggttttgtcg cccactttca gccccagcag
    51121 gtgttgctcc agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc
    51181 gttgttgcga gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
    51241 gacggcgcta ttgctctgta cagattcaga catgaaggtt tgctcttatt tttgaggatt
    51301 tttgccggat gcggcctacg atttcgtagc aatctgtagg ccgcatccgg cagggtttat
    51361 tattgttttt tcgctttgga aggtaaaaac ccttccagca cgatcagtgc cgcaccgaca
    51421 caaatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg
    51481 atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca
    51541 ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc
    51601 actgccagga gcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg
    51661 ccgctatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc
    51721 gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg
    51781 cccagatcga taatcagcac gactaccacc agccacagcc agcgtagccc tgttgaacag
    51841 atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac
    51901 agcggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc
    51961 agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagccct ttgagtactt
    52021 cgctctgctg agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca
    52081 acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt tccggttctg
    52141 catacaaggt tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct
    52201 caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca
    52261 ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt
    52321 tttcacgttc gcccggcaga tagccccaca cttcatcagc ggtgaaggag aggattggtg
    52381 ccatccagcg caccagcgct tcagcgatgt gatacagcgc agtctggcag ctacgacgcg
    52441 ccacgctatc cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac
    52501 ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt
    52561 cgtacgcctt gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca
    52621 gtaccaccat ctcttccggt ttcaccatat cttttgctgg atcaaaacca ttcaggtttg
    52681 ccagcaggaa gcgcgcggtg ttacggatac gacgatagct atcggcagca cgtttcagga
    52741 tctcgtcaga aacggccatt tcaccggtgt agtcggttga tgccacccac agacgcagaa
    52801 tatccgcgcc cagtttgttc atcacatcct gcggcgaaac ggtgttgccg atggatttag
    52861 acatcttgcg accctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg
    52921 ctttgccttt catcgcggtg gagatcatta gggaagacat gaaccaacca cggtgctggt
    52981 cagaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acgtcaacaa
    53041 cagaagagtg ggtagatccg gagtcaaacc atacgtcgag agtatccggc actttcacat
    53101 actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac gcctggatgc
    53161 cgtcaacttc aacgcgttta gccacttctt ccatcagttc gagggtacgc ggatgcagct
    53221 cttccgtgtc tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga
    53281 tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga
    53341 tccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc
    53401 tgacgaacca ctgcggtgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg
    53461 gatagctgtg ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa
    53521 cgacgatgtc gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca
    53581 gataagtgcc gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca
    53641 catagtcgtc cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg
    53701 taacgtgatc gccgaggatt gccggaacgt cgaagcccat aaacggatgg gtaaagcgca
    53761 gcagctcaag ctctgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac
    53821 gctgcattac gctttcaacc agatctttcg ccagaatcgc ggcctgaccg tcgatctgca
    53881 ccagcgcata gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc aaagtccacg
    53941 gcgtagtggt ccagatcacc agcgagattg ggccgttaac gttgcttacg ccaaatttcg
    54001 ctttcagcgc atcctgatcg accgcctgga aagcgacgtc aatggacgga gaagttttgt
    54061 cgtaatactc aacttccgct tctgccagtg cagaacggca gtctacgcac cagtgcaccg
    54121 gcttcgcgcc tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg cggatgatgt
    54181 tggcttcagt tttgaagtcc atagtcagat acgggtgcga ccagtcgccc agcacgccca
    54241 gacggataaa gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg cggcacttgg
    54301 cgcggaactc ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca
    54361 gctcgatcgg cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa
    54421 gccctttgga cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa
    54481 tgctgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag
    54541 ccgcacggat gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc
    54601 gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat
    54661 agtcactcat cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg
    54721 gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca
    54781 gttcgtccag cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta
    54841 tatggcgacc gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa
    54901 taccggcaac cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc ttttcaccga
    54961 ggcccagcac ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta
    55021 cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg
    55081 agatggcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca tcagccagcg
    55141 cctgacgcac ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt tgcgtactgg
    55201 tgatatcgaa gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac
    55261 cagcgccaaa gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca
    55321 aaagatcgct gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg aaacgcacgc
    55381 acagcacgta atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca
    55441 gtcttgccgg ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca
    55501 ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat
    55561 gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct
    55621 ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt
    55681 ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag
    55741 aagtggcttt tcattgccat ggcgcaaatc acgggaagaa actgaccgcc tgctgcaatt
    55801 tttatcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta
    55861 cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg
    55921 ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga
    55981 ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc
    56041 acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg
    56101 aagctggcga caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc
    56161 gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga
    56221 ctgcacagat caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg
    56281 cgcaagcggg tttttttatg cctgctgctt ttgtgatgcg tgagagaaca cactgccggt
    56341 tgccagatag tcaaaagaga catgccgttg tactatctga actcttcgtg gcttaagaat
    56401 cgtagaacca tcggctgata cattctgaac aagaatatct actcaatctc cagactcccc
    56461 cacaaagaat atggatattg tgatacacat tgaggtatta aacttaagac cactctattt
    56521 ttgaattaaa ttcctatata ctttaaccat tcataaatat ctcctcatta gacct
//
LOCUS       DAJTRQ010000013        53191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000013 DAJTRQ010000000
VERSION     DAJTRQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..303)
                     /gene="atl"
                     /locus_tag="OO332_000885"
     CDS             complement(<1..303)
                     /gene="atl"
                     /locus_tag="OO332_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein"
                     /protein_id="HCR5941462.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRW"
     gene            466..562
                     /gene="ffs"
                     /locus_tag="OO332_000886"
     ncRNA           466..562
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO332_000886"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            682..1035
                     /locus_tag="OO332_000887"
     CDS             682..1035
                     /locus_tag="OO332_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5941463.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1077..2627)
                     /gene="pdeB"
                     /locus_tag="OO332_000888"
     CDS             complement(1077..2627)
                     /gene="pdeB"
                     /locus_tag="OO332_000888"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5941464.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2791..3261)
                     /locus_tag="OO332_000889"
     CDS             complement(2791..3261)
                     /locus_tag="OO332_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5941465.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3377..3928)
                     /gene="maa"
                     /locus_tag="OO332_000890"
     CDS             complement(3377..3928)
                     /gene="maa"
                     /locus_tag="OO332_000890"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5941466.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4100..4318)
                     /gene="hha"
                     /locus_tag="OO332_000891"
     CDS             complement(4100..4318)
                     /gene="hha"
                     /locus_tag="OO332_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5941467.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4344..4718)
                     /gene="tomB"
                     /locus_tag="OO332_000892"
     CDS             complement(4344..4718)
                     /gene="tomB"
                     /locus_tag="OO332_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5941468.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5264..8413)
                     /locus_tag="OO332_000893"
     CDS             complement(5264..8413)
                     /locus_tag="OO332_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5941469.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8436..9629)
                     /gene="acrA"
                     /locus_tag="OO332_000894"
     CDS             complement(8436..9629)
                     /gene="acrA"
                     /locus_tag="OO332_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5941470.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9771..10418
                     /gene="acrR"
                     /locus_tag="OO332_000895"
     CDS             9771..10418
                     /gene="acrR"
                     /locus_tag="OO332_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5941471.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10546..13908
                     /gene="mscK"
                     /locus_tag="OO332_000896"
     CDS             10546..13908
                     /gene="mscK"
                     /locus_tag="OO332_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5941472.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14120..14281)
                     /gene="rsmS"
                     /locus_tag="OO332_000897"
     CDS             complement(14120..14281)
                     /gene="rsmS"
                     /locus_tag="OO332_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5941473.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14295..14822)
                     /gene="priC"
                     /locus_tag="OO332_000898"
     CDS             complement(14295..14822)
                     /gene="priC"
                     /locus_tag="OO332_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5941474.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14892..15269
                     /locus_tag="OO332_000899"
     CDS             14892..15269
                     /locus_tag="OO332_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5941475.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15422..15973
                     /gene="apt"
                     /locus_tag="OO332_000900"
     CDS             15422..15973
                     /gene="apt"
                     /locus_tag="OO332_000900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5941476.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16102..18027
                     /gene="dnaX"
                     /locus_tag="OO332_000901"
     CDS             16102..18027
                     /gene="dnaX"
                     /locus_tag="OO332_000901"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5941477.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17360..17424
                     /gene="dnaX"
                     /locus_tag="OO332_000901"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18080..18409
                     /locus_tag="OO332_000902"
     CDS             18080..18409
                     /locus_tag="OO332_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5941478.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18409..19014
                     /gene="recR"
                     /locus_tag="OO332_000903"
     CDS             18409..19014
                     /gene="recR"
                     /locus_tag="OO332_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5941479.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19124..20998
                     /gene="htpG"
                     /locus_tag="OO332_000904"
     CDS             19124..20998
                     /gene="htpG"
                     /locus_tag="OO332_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5941480.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21179..21823
                     /gene="adk"
                     /locus_tag="OO332_000905"
     CDS             21179..21823
                     /gene="adk"
                     /locus_tag="OO332_000905"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5941481.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21955..22917
                     /gene="hemH"
                     /locus_tag="OO332_000906"
     CDS             21955..22917
                     /gene="hemH"
                     /locus_tag="OO332_000906"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5941482.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22914..23874)
                     /gene="aes"
                     /locus_tag="OO332_000907"
                     /pseudo
     CDS             complement(22914..23874)
                     /gene="aes"
                     /locus_tag="OO332_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24026..25330
                     /gene="gsk"
                     /locus_tag="OO332_000908"
     CDS             24026..25330
                     /gene="gsk"
                     /locus_tag="OO332_000908"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5941483.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25463..27139)
                     /locus_tag="OO332_000909"
     CDS             complement(25463..27139)
                     /locus_tag="OO332_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5941484.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27377..28597)
                     /gene="fsr"
                     /locus_tag="OO332_000910"
     CDS             complement(27377..28597)
                     /gene="fsr"
                     /locus_tag="OO332_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5941485.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28815..30467
                     /gene="ushA"
                     /locus_tag="OO332_000911"
     CDS             28815..30467
                     /gene="ushA"
                     /locus_tag="OO332_000911"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5941486.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30504..30983)
                     /gene="ybaK"
                     /locus_tag="OO332_000912"
     CDS             complement(30504..30983)
                     /gene="ybaK"
                     /locus_tag="OO332_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5941487.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31187..31981)
                     /locus_tag="OO332_000913"
     CDS             complement(31187..31981)
                     /locus_tag="OO332_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5941488.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32119..32460
                     /locus_tag="OO332_000914"
     CDS             32119..32460
                     /locus_tag="OO332_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5941489.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32675..35179)
                     /gene="copA"
                     /locus_tag="OO332_000915"
     CDS             complement(32675..35179)
                     /gene="copA"
                     /locus_tag="OO332_000915"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5941490.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35441..36373
                     /gene="glsA"
                     /locus_tag="OO332_000916"
     CDS             35441..36373
                     /gene="glsA"
                     /locus_tag="OO332_000916"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5941491.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36376..37668
                     /locus_tag="OO332_000917"
     CDS             36376..37668
                     /locus_tag="OO332_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5941492.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37793..38200
                     /gene="cueR"
                     /locus_tag="OO332_000918"
     CDS             37793..38200
                     /gene="cueR"
                     /locus_tag="OO332_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5941493.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38201..38659)
                     /locus_tag="OO332_000919"
     CDS             complement(38201..38659)
                     /locus_tag="OO332_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5941494.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38656..39573)
                     /locus_tag="OO332_000920"
     CDS             complement(38656..39573)
                     /locus_tag="OO332_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5941495.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39719..40396
                     /gene="fetA"
                     /locus_tag="OO332_000921"
     CDS             39719..40396
                     /gene="fetA"
                     /locus_tag="OO332_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5941496.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40383..41162
                     /gene="fetB"
                     /locus_tag="OO332_000922"
     CDS             40383..41162
                     /gene="fetB"
                     /locus_tag="OO332_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5941497.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41225..42079)
                     /gene="cnoX"
                     /locus_tag="OO332_000923"
     CDS             complement(41225..42079)
                     /gene="cnoX"
                     /locus_tag="OO332_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5941498.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42140..42949)
                     /gene="ybbO"
                     /locus_tag="OO332_000924"
     CDS             complement(42140..42949)
                     /gene="ybbO"
                     /locus_tag="OO332_000924"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5941499.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42939..43561)
                     /gene="tesA"
                     /locus_tag="OO332_000925"
                     /pseudo
     CDS             complement(42939..43561)
                     /gene="tesA"
                     /locus_tag="OO332_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43532..44218
                     /locus_tag="OO332_000926"
     CDS             43532..44218
                     /locus_tag="OO332_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5941500.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44215..46629
                     /locus_tag="OO332_000927"
     CDS             44215..46629
                     /locus_tag="OO332_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5941501.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47341..48435)
                     /gene="mnmH"
                     /locus_tag="OO332_000928"
     CDS             complement(47341..48435)
                     /gene="mnmH"
                     /locus_tag="OO332_000928"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5941502.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48504..49430)
                     /gene="allS"
                     /locus_tag="OO332_000929"
     CDS             complement(48504..49430)
                     /gene="allS"
                     /locus_tag="OO332_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5941503.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49660..50142
                     /gene="allA"
                     /locus_tag="OO332_000930"
     CDS             49660..50142
                     /gene="allA"
                     /locus_tag="OO332_000930"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5941504.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50220..51035
                     /gene="allR"
                     /locus_tag="OO332_000931"
     CDS             50220..51035
                     /gene="allR"
                     /locus_tag="OO332_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5941505.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51125..52906
                     /gene="gcl"
                     /locus_tag="OO332_000932"
     CDS             51125..52906
                     /gene="gcl"
                     /locus_tag="OO332_000932"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5941506.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52919..>53191
                     /locus_tag="OO332_000933"
     CDS             52919..>53191
                     /locus_tag="OO332_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5941507.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12817 a        13703 c        14011 g        12660 t
ORIGIN      
        1 ccagcgataa cgctgcaagt cgatttgccc gcttcccgat accatcacac cttctgccag
       61 taatgcctgt cgctgacgct gtaaatccgg cccggttagc gaaattgtgc catggcgatt
      121 aaccacccgg tgccagggta aggtgctgcc ttcagggaga cgctttaaca caccgcccac
      181 ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca tcaccgtaag tggtgacata
      241 gccttcggga atagcggcga cgatttgcca gacgcgttgg ggaaatgaat cttctttttc
      301 catcttttct tcctgaggta atttttcagc ataatctgga aaaacgcccg agtgaagtcg
      361 cattgcgcaa gaaaccagca tctggcacgc aatgggttgc aattagccgg ggcagcagtg
      421 ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg ggctctgttg gttctcccgc
      481 aacgctactc tgtttaccag gtcaggtccg gaaggaagca gccaaggcag atgacgcgtg
      541 tgccgggatg tagctggcag ggcccccacc catttctgcc tcccaccgtt tcgtcaaaaa
      601 atcccagcat ggctaaactt taaccacaac tgacgtcgca agaattgtct ggctgcgcag
      661 tacgcttcgg aggtatgtct gatgaagtat gttgatggtt ttgtggttgc cgttcctgcc
      721 gataaaaagg atgcctatcg ggaaatggcc gctaaggccg cgccattgtt taaagagttt
      781 ggcgcgcttc gtattgtcga atgctgggcc agcgatgtac cggatggcaa agtgaccgat
      841 tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg tctttagctg gattgaatac
      901 ccttcaaaag aggtccgcga cgcagctaat caaaagatga tgtcggaccc acggatgaaa
      961 gagttcggcg agtccatgcc gtttgacggc aagcgaatga tctatggcgg attcgagtca
     1021 atcatcgacg aataggtagc gtgacgggct gtgcggcgtg ccgtcagccc aaaaattcac
     1081 aaatgttgct cggcccagcg taagaaatct tctttcggta atgccttgct gtagagccag
     1141 ccctgaccgt agtgcacgcc atgctggcgt agccactctt cttgtttact ggtttcgatt
     1201 ccctccgcta ccattttcag tttcagtgtt tttgccattt cgatgatatg cggcgtgaca
     1261 tttttatatt ccagcgcatc aacgaaagat ttatcgatct tcagaatgtc gacatccaga
     1321 tcctgtaaat aacttaaact tgaatacccc gtaccaaaat catcaagata aatttcatgg
     1381 cccgcctccc ggtagcgaga aattatcggg gcgctggttt tcggatcggc aaactcgcgt
     1441 tcagtgagtt caagcgcgat ctgtctggga ttaacctgat agtgattgat catttcacgc
     1501 agcaattgcg ggattttttc cgaggtgagc acggtggatt caagattgat cgaaatatgc
     1561 tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa agacgcttct tataatcagt
     1621 agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg gaataaaact atctggtgac
     1681 aaccaactac cgtctgtctg cggccagcgc gccagtgcct cagcaccgac aattttgcca
     1741 ttggctaagg agacaatcgg ctgatagtgc acgcaaatat cacgattttc gatagcatcc
     1801 tgcagccgat gatgcggtga ctggatacgg cgcaaaatac gcagcacaaa catcgctgcc
     1861 agcaggccaa tcaccaaccc gagcggtaac caaataaaga cttgccgatg ccagcctttc
     1921 tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat tcatctccgg gaagggcagg
     1981 atatcgtaaa tgatcccatt attttcgata tgctcaccgg gtgttttttg tagcctggta
     2041 ataattccct gagcaatttc atcgctgctg gttatgacaa cgttatgggc attgccaata
     2101 atggcggcat caatttgcca tgagctatag ggaatgacat caataaagga agcggggtcg
     2161 atcatgacga cataatgtgc cgttcccatg gcgaccatgt aacggataat gcctaaatcg
     2221 ttatgcgatg ttaaccagac acgataacca tctttcgaaa ttttacctgg ctcgggaaag
     2281 gtatcgggcg gactttcatg ctccagagac gaacactggg gaacgttgtt atcgatataa
     2341 accacttcct gaatatagcg ataactgtaa gatacccgac gcatttccat gagatgggct
     2401 tcgctacagg cagcgccttg ccatctttcc agctcctgca gcgcatcttt cccttgtgtc
     2461 gccaccttat tggctcgaat agcgacgcgg gaggaatagg tatccagctc ttcaataaac
     2521 gatgtttcta cctgctgatg ggccagccag atgcttaagc cgacaggcag caatactgaa
     2581 agaatcagta ctcccgaaat aaggccgacc agatgtcgtg ttctcaccag catatcctta
     2641 ttaacacagt gtagggaaag tatatctgat tgtggaaaaa ggctggcagg agataaggag
     2701 gtagagctgg tatttcatga agtgatgggt ggaaacgatc attgtcattt gaccaggcgc
     2761 agagctttac gcctggtatg taaccctaac ttaagatgtt gattcggcgc gagcgagggt
     2821 aaaaatatcg taaaaagaca gttcaccttt acggacgatc attttttgca gttgttcctt
     2881 atgaacatcc gcgacacgcg gagagttcaa gatggcttca accagcgcgg caggtacaaa
     2941 gcgcgtgttg taccattcgc cgttatagca aacgcggaaa tccagcacgt cgatatcttc
     3001 atagcggtag cgtgggtaga catcgaaaaa gaagaaacca ttaagcagga taaataatac
     3061 aaccagtagc cagacagagc ctgacagcgt ttcggactgc aacatcaccg ccagggtggc
     3121 aaaaaaagca acgtacatac cgataaacag ccccggatgt ttacggataa aactgatact
     3181 aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga gactcaatcg tttcggtcag
     3241 caggcgttgt atttcggtca tcgtttgcct tcgtggtatc tgcaactttc cagggaacac
     3301 cctattttaa cgtgggaata gtagagggga agtaacagat ctaccacaat tttgcataac
     3361 agttgcgaaa aaccgattac aattttttaa ttattctggc tggattaccg cccacgacaa
     3421 cgttgtccgg gacacctttt gtgacaactg cacctgaagc taccacgacg ttatcaccaa
     3481 tggtcacacc agggttaatg accgcgcgtc cgccaatcca gacgttatta ccgatggtga
     3541 cgggtttccc cagttcagca ccgctattac gtgctacagg gtcgatggga tgtgttgccg
     3601 tgtagatatg aacgcctggt gccaacatac agttatcacc gatgcgaata gggcagacat
     3661 caagcatcac gcaatcgaag ttggcgaaaa aattattgcc gagaaaaatg ttatagccat
     3721 aatcacagcg aaacgttggc tcaatataag cctctgtcac ctgaccgaat aaatcagcga
     3781 gaatttgctg gcgtaatgtg tgctcttccg ctagggaatg attgtatcgg tgaataagct
     3841 gacgagcgcg caggcgatcg cgagataacg tctcatctgc cgagcgatac aactcaccag
     3901 caatcatctt ttctttttct gtgctcatta ctgacctccg gttgctgaaa atgcggcaac
     3961 acagtaagac gaaaagtcag tgaagagaac gttctcatta catctgtgtg ataaagatca
     4021 catagggtat gtcttcatgg cgaaaaagta taaaattctt aataaacagc cggttatagc
     4081 tccgaaagcg aattatttat tagcgaataa atttccatac tgaggaaggg atcttgtcgt
     4141 acagtttatt catggtcaat tcggcgaggc ggtgatctgc ggctgagtaa aataccgcca
     4201 gttcattatc tgataattcg tatttatttt tctcgataac acgctccagc gtgtcaattg
     4261 tctggcaacg acgtaaacgc attaaataat cggtttttgt taaaggtttt tcggacatac
     4321 ttctacccat ggttgtaata attctaacaa gataaactcg caggattctc tttcgtcgca
     4381 ttgacaaaac aacggaatag tcgattacct gacttccgcc atttctgaag agcctgcata
     4441 ttaataccat aactgctgaa caacataaag gtgtcatcca gatattcgtc gatctgctca
     4501 atgagcttat tgtcttcatt atacttaatt ttgtaattaa gtgcgaaggt cgcaatatgc
     4561 tcaatcagtt cattcaactg gaggttgatc gccgaggttg ggtcgttaac ccagccatga
     4621 ttgctttctt caaggtttgc aagacagtca tgatacaggg tttcacagag aaacttaagc
     4681 tgtgcgatat catgtctttt gggtgagtat tcatccataa cgcgtcccct tcttagtggt
     4741 tgaactaacg gacacctttc gggatggaaa aaacttactg acctagactt gccctctcgt
     4801 ttgttagctt aactataagc cactctttgc aggttttcat cgcattatta cgaaacatta
     4861 caattaggaa ggaatcggga caggactcac tttttcatac tagcactaac ccgcagcagg
     4921 taaaagcagt ttgctcgaac tgttcataac atttcaggat tatcgccaga tgaaattggt
     4981 gacactaata ccaggattgc tctgaatatg acgtaataac cgaggaatga ataaagaatt
     5041 accgcaaata attaagaata gcctctaaat gattatggat tattaaatct attagcgcta
     5101 cttaatataa attaactaac aatcagtatt ttttatgaat tttatacgtg gttaatactg
     5161 gttttcgtat gagatcctga gttggtggtt caattactcc ttaatgttcg taggttatgc
     5221 ataaaaaagg ccgcttacgc ggccttagtg attacatgtt gtatcaatga tgatcgacag
     5281 tatggctgtg ctcgatatct tcattcttgc ggctaaagcg gcggcgaacc accacaaaga
     5341 ataccggaac gaagaagatt gccagtaccg ttgcggtcac catcccgccc attacaccgg
     5401 tacctactgc gttctgcgcg ccggaaccag caccagtact gataaccagc ggcataacgc
     5461 cgaggataaa cgccagcgag gtcatcagga tcggacgtaa acgcatccgc accgcatcaa
     5521 gcgtcgcttc aatcagacct ttaccttctt tatccatcaa gtctttggcg aattcgacga
     5581 taagtatcgc gttcttcgcc gacaacccaa tggttgtgag cagtcctact tggaagtaaa
     5641 cgtcattggt caggccacgg aaggtggcag ccagcaacgc accgataacc cccagcggaa
     5701 cgaccagcat aacggagaac ggagtcgacc agctctcgta cagcgccgcc agacacagga
     5761 atacgacaat caacgaaacc gcgtacagtg aaggtgcctg gttgccggag agacgttcct
     5821 gataggacat ccccgtccag tcatagccaa caccggtagg cagtttgctc gccagttgtt
     5881 ccatcagctc cattgcttca ccggtacttt tacccggtgc cgcctggcct aagatttcca
     5941 tggatggcag gccgttgtaa cgttccagac gcggcgaacc gtactcccaa cgagaagagg
     6001 agaacgccga gaatggcacc atctgaccat cagcagcacg aacataccag tcgccgatat
     6061 catccggcag catacggtat ttcgcttctg acatgacgta aactttcttc acacgaccgc
     6121 ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc gcccagagtg gtgttaatgt
     6181 cgttgataga aacacccagc gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg
     6241 gggtatcttc cagaccgttt ggacgtacgc tggtcagcat atcagggtgc ttcgctgctt
     6301 ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg accaaggcca gcctggtcaa
     6361 tcagctcaaa gtcaaagccg gttgcagtac ccagttccac gattgcgggc aggttaaagg
     6421 cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt tgcacgcatg gtaatcgctt
     6481 caactttgtt ttcttcgccc ggacgatcgg cccagtcctt caaggaaacg aacgcaatac
     6541 cggtattctg accacgtccc gcaaagccga agccgttaac ggcgaacacc gactcaacgt
     6601 tgtttttttc tttggtcaga tagtaatgcg ttacctcatt gagcactttc tgcgtacgtt
     6661 cctgcgttgc acctgctggc agctgaacca tggtcataaa tacgccctgg tcctcatctg
     6721 gcaagaagga gcttggcaga cgcacgaaca gataggccat gccgaccacg atgatcagat
     6781 acagcactag gtaacgcccc gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt
     6841 gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa gcctttttta ccttccccgt
     6901 gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag agccggagtc aggatcaatg
     6961 ctaccagtac cgacagcgcc attgctgaaa caatggtaat agagaactga cgatagatag
     7021 caccagtaga accgccaaag aaggccatcg gtacgaatac cgccgacagt accatcgcga
     7081 taccgaccag agcgccctga atctgcccca tcgacttacg ggtagcttct tttggcggca
     7141 aaccttcttc cgccataaca cgctccacgt tttctaccac aacgatagcg tcatccacca
     7201 acaagccgat ggcgagcacc atcccgaaca ttgttagcgt gtttatcgag aagccaaagg
     7261 cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg
     7321 tcgcgcggaa gttctgcagg aacagataca taaccaggaa cacgaggatg atcgcttcga
     7381 ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa cggcgtggtg tcgtacgggt
     7441 aaacaatttt cagacccgac gggaagaacg gttccatctt cgccagttca gcacggattg
     7501 ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt gatccccaga ccggaagccg
     7561 gttggccgtt aaactctgcg atgatgtcgt agttctcacc acccagctca atcttcgcca
     7621 catcacgcag cagtacgcgg gaaccatcct gattcacttt cagcaggatt ttgccgaact
     7681 cttcagtaga ggtcagacgc gtctgagcaa taatagaggc gttaagctgt tggcctttca
     7741 ccggcggcgt accaccgagc tgacccgccg caacctgggc gttctgcgct ttgatggcgg
     7801 taatgacatc aaccggcgtt agctggaatt tgttcagctc attcgggttc atccagatac
     7861 gcatcgcgta ctgtgaaccg aacaactgaa catcacccac gcccgacgta cggctgatgg
     7921 catctttcat attcgccgct acgtagtcag agatatcctc ctgcgtcatg gtgccatcgg
     7981 tgttgataac gccgacaacc atcaggaagc tgctggatga tttctcaacg ctcacccctt
     8041 gctgctgaac ttcttgcggc agcaacggca tcgccagctg cagtttgttc tgcacctgaa
     8101 cctgcgcgat atccgcatca gtaccagact caaaggtcag ggtgatctgc acggtacccg
     8161 tggagtcact gttagaggac atgtacatca ggttatcgat accgttcata ttctgttcga
     8221 taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc gccggggtag gaggcggaga
     8281 tcgttactgc cggcggtgca atcgtaggat attgtgccac cggcagtttg aggatcgcca
     8341 gcccccctgc caacatgatg ataatggcga tcacccacgc aaaaatcggg cgatcgataa
     8401 agaaattagg catgtcttaa cggctcctgt ttaagttaag acttggactg ttcaggctga
     8461 gcaccgcttg cggcttgctg gttattatca gcggtaactt cttgtgcttt tacctggaca
     8521 ccaggacgca ctttctgcag cccacttatt actacgcgat cgcctgcttt cagaccttct
     8581 gtcaccagcc acttatcgcc aatagcctgg cttgcaacga tcggacgggt ttccactttg
     8641 tcatccgcgc caactaccag tacggtggca tcaccacgcg gcgtacgggt tacgccctgt
     8701 tgcgggacta aaatagcgtt tggattaagc ccttcttcca gacgtgcgcg cacgaacata
     8761 cccggcagca gagtgtgatc cgggttcggg aagatagcgc gtagggtgat agacccagtg
     8821 gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt cctgcgggaa cttaatgccg
     8881 tcactggtga tcagcgacac tttggctttg ccgttctctt gtttcagcgt gccattcgcc
     8941 agttcctgtt tcaggcgcag gaagtcgttg ctggactggg tcacatcaac gtagatcgga
     9001 tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc
     9061 gtcacgttcg acttaccaat gcgaccgcta atcggagagg tgactttggt gtaagccaga
     9121 ttgatccgcg cagtttcaac ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc
     9181 gcatcagcca gagcctgatc gtacccttgc ttactgatgt actgagtacc gagcagtttc
     9241 tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag cctgggcttt cgccagatca
     9301 cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat caatctgata gagagagaca
     9361 ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca ggataatccc gctaacttga
     9421 ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc
     9481 agaggttcag ttttgactgt tactacgcca acggcgggca tctgctggcc accttgttgg
     9541 gcctgtttgt cgtcacatcc tgttagggct aagctgcctg agagcatcag aacgaccgcc
     9601 agaggcgtaa accctctgtt tttgttcata tgtaaacctc gagtgtccga tttcaaattg
     9661 gtcaatggtc aaaagttaat aaacccattg ctgcgtttat attatcgtcg tgctatggta
     9721 catacattca caaatgtatg taaatctaac gcctgtaaat tcacgaacat atggcacgaa
     9781 aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt
     9841 tctcacagca gggggtatca tccacctcgc tgggcgagat tgcaaaagca gctggcgtta
     9901 cgcgcggtgc aatctactgg cattttaaag acaagtcgga tttgttcagt gagatctggg
     9961 aactgtcaga atccaatatt ggtgaactag agcttgagta tcaggcaaaa ttccctggcg
    10021 atccactctc agtattaaga gagatattaa ttcatgttct tgaatccacg gtgacagaag
    10081 aacgacgtcg attattgatg gagattatat tccacaaatg cgaatttgtc ggagaaatgg
    10141 ctgttgtgca acaggcacaa cgtaatctct gtctggaaag ttatgaccgt atagaacaaa
    10201 cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga tttaatgacg cgtcgggcag
    10261 caattattat gcgcggctat atttccggcc tgatggaaaa ctggctcttt gccccgcaat
    10321 cttttgatct taaaaaagaa gcccgcgatt acgtcgccat cttactggag atgtatctcc
    10381 tgtgccccac gcttcgtaat cctgctacta acgaataacc ctgaatctga ctccaggatt
    10441 tttcctggac attttcgtcg ttgctattct ggttcactgc gtcgtgatat tcttgcggtt
    10501 tgactttttc aggtcgttct tcaggttcag aaaccttcat tcatcatgac tatgttccag
    10561 tattacaaac gatcacggca ttttgttttt tcagcattta ttgcttttgt ttttgtcttg
    10621 ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg gtgatctgcc gacaaaagcg
    10681 gacctgcagg cgcaacttga ctcactaaat aaacaaaaag atctttctgc tcaggacaaa
    10741 ctggtgcagc aggatctgac agatacatta gccaccctcg ataaaatcga tcgcataaaa
    10801 gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg
    10861 accgcggcgt taacagcact tagcgatgtc gataacgacg aagaaacgcg caaaattctg
    10921 agtacgcttt cgttgcgcca gctggaaact cgcgttgccc aggcgctgga cgatttgcaa
    10981 aacgcacaaa acgatctggc gtcttataac agccagctgg tttcgttaca gacgcagccc
    11041 gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt
    11101 ctggatggga ctgatgtcgg cgagactgcc ttacgtccca gccagaaagt gttaatgcag
    11161 gctcagcagg cgttgctgaa tgcggagatt gaccagcagc gtaaaagcct ggaagggaac
    11221 accgtcttgc aggatacctt gcaaaagcaa cgtgattacg tgacggcgaa cagcgcccgt
    11281 ctggagcatc agttgcagct gttgcaagaa gcggtaaaca gcaagcgcct gactttaact
    11341 gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg cgcgtattca ggctaatccg
    11401 ctggtgaaac aggaactgga aattaaccag cagttaagtc agcgtctgat taccgcgact
    11461 gaaaacggta atcagttgat gcagcaaaac attaaagtca aaaactggct ggagcgggcg
    11521 ctgcaatcgg aacgcaatat taaagagcag attgccgtcc tgaagggcag cctgctgttg
    11581 tctcgtatcc tttaccagca acaacaaacg ctgccctcgg cggatgaact ggaaaacatg
    11641 accaaccgca tcgcggattt gcgtctcgaa cagtttgaag ttaaccagca gcgtgatgca
    11701 ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag gtcacaccaa cgaagtcaac
    11761 agcgaagttc acgatgcgtt attgcaagtg gttgatatgc gtcgcgaatt gctggatcaa
    11821 ctcaacaaac agttgggtaa ccagctgatg atggccatta acctgcaaat caaccagcag
    11881 cagttaatga gcgtatcgaa aaacctgaaa tccatcctga ctcagcaaat cttttgggtg
    11941 aacagtaacc gtccaatgga ctgggactgg atcaaagcgt tcccgcaaag cctgaaagat
    12001 gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag cctggcctgc cgtttttatc
    12061 gctttcctcg ccggtttgcc gctgctgttg attgccgggc tgatccactg gcgtctgggc
    12121 tggctgaaag cgtatcaaca gaaactggct tccgctgtgg gttccctgcg taacgatagc
    12181 cagctcaaca caccaaaagc gatccttatc gacctgatcc gcgcgctgcc ggtgtgcctg
    12241 attattctcg cggttggcct gattctgttg accatgcagc tcaatatcag cgaactgcta
    12301 tggtcgttca gtaaaaaact ggcgattttc tggctggtgt ttggcctgtg ctggaaggta
    12361 ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc cggagcagca gaccagccac
    12421 tggcgtcggc aaattgtccg catcagtctc gcgttgctgc ctatccattt ctggtctgtg
    12481 gtggcagaac tttccccgct gcatctgatg gatgatgtgc tggggcaggc gatgattttc
    12541 ttcaacctgc tgctgattgc cttcctggta tggccaatgt gccgcgaaag ctggcgtgat
    12601 aaagagtcgc acaccatgcg actagtcact attaccgtgc tgtcgattat cccgattgca
    12661 ttgatggtgc tgactgccac gggttatttc tacaccacgc tgcgcctctc cgggcgttgg
    12721 attgaaaccg tttatctggt gatcatctgg aacttgctct accagacggt attgcgcggc
    12781 ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc tggcgcgtcg gcagaatctg
    12841 gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac ccaccattgc actggagcaa
    12901 gttaaccagc agacgctgcg tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg
    12961 ttctgggcaa tttggtccga tttgatcacc gtgttcagct atctcgacag catcacgctc
    13021 tggcattaca acggcactga agctggcgct gcggtggtga aaaacgtcac catgggcagt
    13081 ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt
    13141 ttactggaag tgctggtgct ctcgcgactg aatatgcgcc agggcgcgtc gtatgccatt
    13201 actaccatcc ttaactacat cattattgcc gttggtgcga tgacggtgtt cggatcgctg
    13261 ggcgtctctt gggataaact ccagtggctg gccgcagcat tatccgtagg tcttggtttt
    13321 ggtttacaag aaattttcgg taacttcgtc tccggtttga tcattctgtt cgaacgtccg
    13381 gtgcgtattg gcgatacggt aaccattggt agcttctcgg ggacggtaag taagatccgt
    13441 attcgtgcga caacgattac cgatttcgat cgcaaagaag tgatcatccc gaacaaagcg
    13501 tttgttaccg agcgtctgat caactggtcg ttgactgaca ctactacgcg tctggtgatc
    13561 cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc gtaaagtgtt actgaaggcg
    13621 gcgactgagc acccaagggt gatgcacgaa ccaatgccgg aagtcttctt tacggcattt
    13681 ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc gtgaactgcg tgaccgtagc
    13741 cgtactgtcg atgagctgaa ccgtactatc gatcagctgt gccgtgaaaa cgacatcaac
    13801 attgccttta accagcttga agtgcatctg cacaacgaga agggcgatga ggtgacggaa
    13861 gtaaaacgcg actacaaagg cgacgatccg acaccagcgg ttgggtaata accaaagggg
    13921 gcgacattta gttgccccga gattactgac aaagtgcgcg ttgttcatgc cggatgcggc
    13981 gtgaccgcct tatccggcct acgaaaccgc aagaattcaa tatattgcag gagcggtgta
    14041 ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc ccgcaatctt aggggacatt
    14101 tagcggcccc atttatttct cacttttccg cctcatcatc gtgcgttaat ttcttttcat
    14161 aatcacgctt tacaatatcc agcgcgcgca gcacggtact ggcagggatc tgattttcct
    14221 ccagcagcac aatcaaatcg acagccagtt tgacatcgtc aggggcattt tcaagtgaca
    14281 tactctttcc attgctagcg ggttaagcgc gctagcctgt tttcgatttt ttccagcgca
    14341 tggcggcagc gtgccaggcg cgcttcatag gcttccactt cacgatgcag cgtttgctgt
    14401 tccacgagat cggtcacccg cgccaggcgg gctctgcgtt cggcaaccat ctcacgtagc
    14461 cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc agcgggcaat tttcggtggc
    14521 gcactgtccc actcgcgcaa tgaccaggcg gcggcttcac gcgcgatggc ttccagttgt
    14581 gccgccagat gttccgccag ccaggccact tgcggcagct gttgctgctc aactgcatga
    14641 cgaagcgcag ccagattatc gcccgcctcg tcgagacaag cctgtagtgt tgtcgcacga
    14701 gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg cgaactgtga caccggggca
    14761 caacgctgac gcagcgtagc gagctgtcct tccagttttt ccagcagcag ggcggttttc
    14821 acgataagac ctcaatgaaa atgataattg ttatgctaaa gtagccactc tgtaagctga
    14881 caacactcaa tatgcaacga atcattttaa tcatcattgg ctggctggcg gtagtgctgg
    14941 gtacgctggg cgtggtatta ccggtattac cgacgacgcc gtttatcctg ctggcggcct
    15001 ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg
    15061 gcagctatct acgtttctgg cagaaacatc atgcgatgcc gcgcggcgtc aaaccgcggg
    15121 cgattttgct tattttgctc acgtttgcca tttctctgtg gttcgtccag atgccatggg
    15181 tgcgcatcat gttgctggta attctcgcct gtttgctttt ctatatgtgg cgaattccgg
    15241 tgattgatga aaagcaagaa aagcactgaa gcacaacaat cgcagttgca attattgcgt
    15301 acagccagta cattctggcg ttttcgagca caggcgcagg cggtcaaagg ttaaacaacg
    15361 gttacttttg atacgtttaa aacgcgccgt gagtaccacc gtaacaagca ggcatacact
    15421 tatgaccgcg actgcacagc agcttgagta tctcaaaaat agcatcaaaa gcattcagga
    15481 ctacccaaaa cccggcattc ttttccgcga tgtcaccagc ttactggaag acccgaaagc
    15541 ttacgctctc agcatcgact tgctggttga gcgttacaaa aatgcgggca ttaccaaagt
    15601 tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt
    15661 tggctttgta ccggtccgta aaccgggcaa actgccgcgt gaaaccatca gtgaaaatta
    15721 cgacctggaa tacggcaccg atcagctgga aatccacgtt gatgccatca aaccgggcga
    15781 caaagttctg gtggtggacg atctgctggc aaccggcggc actatcgaag cgaccgttaa
    15841 actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg ttcattatca acctgttcga
    15901 tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt
    15961 cccgggccat taattatcgc cagtcttgtg ctgcccacgc tacggacagc acaagatgtg
    16021 cattcagcct cgccgttctg acggggctgt gttagcatta ccccttcgtg aatccacctt
    16081 ccagcgtttc agagcctgcc aatgagttat caggttttag cccgaaaatg gcgcccacaa
    16141 acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg
    16201 ttagggcgta ttcatcatgc ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct
    16261 atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg gcattaccgc gacgccgtgc
    16321 ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct ttgtcgatct gattgaaatc
    16381 gacgccgcct cgcgcaccaa agttgaagat acccgcgacc tgctggataa cgtccagtac
    16441 gctccggcgc gtggtcgttt caaagtttat ctgatcgacg aagtgcatat gctgtcgcgc
    16501 cacagcttta acgcactgtt aaaaaccctt gaagagccgc cggagcacgt taagtttctg
    16561 ctggcgacga ccgatccaca gaaattgccg gtgacgattt tgtcacgctg tctgcaattt
    16621 catctcaagg cgctggatgt cgagcaaatt cgccatcagc ttgagcacat cctcaatgaa
    16681 gaacatatcg ctcacgagcc gcgggcgctg caattgttgg cgcgcgccgc tgaaggcagc
    16741 ctgcgagatg ccttaagtct gaccgaccag gcgattgcca gcggtgacgg ccaggtttca
    16801 acccaggcgg tcagtgcgat gctgggtacg cttgacgacg atcaggcgct gtcgctggtt
    16861 gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac tgattaatga agccgctgcc
    16921 cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg gcttgttgca tcgtattgcg
    16981 atggtacaac tttcgcctgc tgcacttggc aacgacatgg ccgccatcga gctgcggatg
    17041 cgtgaactgg cgcgcaccat accgccgacg gatattcagc tttactatca gacgctgttg
    17101 attggtcgca aagaattacc gtatgcgccg gaccgccgca tgggcgttga gatgacgctg
    17161 ctgcgcgcgc tggcgttcca tccgcgtatg ccgttgcctg agccagaagt gccacgccag
    17221 tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa ccctggtgcc gccgcaatca
    17281 gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca ccagccaggt gctggcggcg
    17341 cgccagcagt tgcagcgcgt gcagggagca accaaagcaa aaaagagtga accggcagcc
    17401 gctacccgcg cgcggccggt gaataacgct gcgctggaaa gactggcttc ggtcaccgat
    17461 cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag cgccagccaa aaaagaagcg
    17521 tatcgctgga aggcgaccac tccggtgatg cagcaaaaag aagtggtcgc cacgccgaag
    17581 gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac tggcggcgaa gctagcggca
    17641 gaagccattg agcgcgaccc gtgggcggca caggtgagcc aactttcgct accaaaactg
    17701 gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg acaacgcagt atgtctgcat
    17761 ttgcgctcct ctcagcggca tttgaacaac cgcggcgcac agcaaaaact ggctgaagcg
    17821 ttgagcatgt taaaaggttc aacggttgaa ctgactatcg ttgaagatga taatcccgcg
    17881 gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag aaaaacttgc gcaggcgcgc
    17941 gagtccatta ttgcggataa taatattcag accctgcgtc ggtttttcga tgcggagctg
    18001 gatgaagaaa gtatccgccc catttgatcg taagcacagc ttacgttcgt catccttaac
    18061 gtgattgaga gagaaaccta tgtttggtaa aggcggtctg ggtaacctga tgaagcaagc
    18121 ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag atcgcgcagc tggaagttac
    18181 cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac ggtgcacaca actgccgtcg
    18241 cgtagagatc gacccgagcc tgctggaaga cgacaaagag atgctggaag acctggtggc
    18301 tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg cagaaagaaa aaatggcctc
    18361 tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg ccgttctgat gcaaaccagc
    18421 ccgctgttaa cacagcttat ggaagcactg cgctgtctgc cgggcgttgg cccgaagtcg
    18481 gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta gcggcgggat gcgtctggcg
    18541 caggcgctca cccgggcgat gtcggaaatc ggccactgcg ccgattgccg cactttcacc
    18601 gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc aggaaaacgg tcaaatctgt
    18661 gtggtggaga gtccggcgga catctacgcc attgagcaga cggggcagtt ttctggtcgt
    18721 tattttgtgt tgatggggca tctgtcaccg ctggacggca tcggtccgga tgatatcggg
    18781 cttgatcgtc tggaacagcg tctggcagag gaaaaaatca ctgaagtgat cctcgccacc
    18841 aatcccacgg ttgaaggtga agctaccgct aactacattg ccgagctttg cgcgcaatat
    18901 gacgtggaag ccagccgaat cgctcatggc gtaccggttg gcggcgaact ggaaatggtc
    18961 gatggcacca cgctgtcaca ctccctcgcc gggcgtcata agattcgttt ttaagcaaac
    19021 gagagcagga gcacctgctc tcgcttgaaa ttattctccc ttgtccccat ctctccctca
    19081 tcctgttttt aaccttaaaa tggcattatt gaggtagacc tacatgaaag gacaagaaac
    19141 tcgtggtttt cagtcagaag tgaaacagct tctgcacctg atgatccatt ctctctattc
    19201 caataaagaa atcttcctgc gtgagcttat ctctaacgcc tccgatgcgg cggacaagct
    19261 gcgtttccgt gcgctatcta acccggacct gtacgaaggt gatggcgaac tgcgcgttcg
    19321 tgtctctttc gataaagaca agcgcacgct gaccatctcc gataacggcg tggggatgac
    19381 ccgcgacgaa gtgattgacc atctggggac tatcgctaaa tccggtacca aatcattcct
    19441 cgaatccctg ggttctgacc aggcgaaaga cagccagctg atcggtcagt ttggtgttgg
    19501 tttctactct gcgtttatcg tggccgacaa agtgaccgtg cgtactcgcg cggcaggcga
    19561 aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa ggtgaatata ccgttgccga
    19621 catcaccaaa gaagatcgtg gtactgaaat caccctgcat ctgcgtgaag gcgaagacga
    19681 gttcctcgat gactggcgcg tgcgttccat catcagcaaa tactccgacc atatcgcgct
    19741 gccggtagag atcgaaaaac gcgaagagaa agatggcgaa accgttatct cctgggcaaa
    19801 aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg gaaatcaccg atgaagagta
    19861 taaagagttc tacaaacaca tcgcccacga ctttaacgat ccgctgacct ggagccacaa
    19921 ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac atcccgtccc aggctccgtg
    19981 ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg tacgttcaac gcgtgtttat
    20041 catggacgac gctgaacagt tcatgccgaa ctatctgcgc ttcgtgcgtg gtctgattga
    20101 ctccagcgat ctgccgctga acgtttcccg tgaaatcctc caggacagca cggtaacgcg
    20161 taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg ctggaaaaac tggcgaaaga
    20221 cgacgcggaa aaataccaga ccttctggca acagtttggc ctggtactga aagaaggtcc
    20281 ggcggaagat ttcgctaacc aggaagcgat cgccaaactg ctgcgttttg cttctactta
    20341 taccgattct tctgcgcaga ccgtgtctct ggaagactac gtttcccgca tgaaagaagg
    20401 gcaggagaaa atctactaca tcactgcaga cagctatgcg gcagcgaaga gcagcccgca
    20461 cctggaactg ctgcgtaaga aaggcatcga agttctgctg ctttccgacc gcatcgatga
    20521 gtggatgatg aactatctga ctgagttcga cggtaaaccg ttccagtcgg tgtctaaagt
    20581 tgacgagtcg ctggaaaaac tggctgacga agttgatgag agcgcgaaag aagcggagaa
    20641 agcactgact ccgttcatcg accgcgtgaa agctctgctc ggcgagcgcg tgaaagatgt
    20701 ccgtctgact caccgtctga ccgatacgcc agcgatcgtt tcgaccgacg cggacgaaat
    20761 gagcactcag atggcgaaac tgttcgctgc ggcgggccag aaagtgccag aagtgaaata
    20821 catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt gcggcagata ctgaagatga
    20881 agccaagttc agcgagtggg tagaactgct gctggatcag gcgctgctgg cagaacgcgg
    20941 cacgctggaa gatccgaacc tgtttattcg tcgtatgaac cagctgctgg tttcctgatg
    21001 tcatgccgga tgacctttgt gtcatccggc atttttcttt tcatcatctg tactttccgc
    21061 aaattatctc gccattaacc gtttcagcct caggtgcctt tcttgaggca atcgcctgtt
    21121 ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat tttaagggga ttttcgcaat
    21181 gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg actcaggctc agttcatcat
    21241 ggagaaatat ggtattccgc aaatctccac tggcgatatg ctgcgtgctg cggtcaaatc
    21301 tggctccgag ctgggtaaac aagcaaaaga cattatggat gctggcaaac tggtcaccga
    21361 cgaactggtg atcgcgctgg ttaaagagcg cattgctcag gaagactgcc gtaatggttt
    21421 cctgttggac ggcttcccgc gtaccattcc gcaggcagac gcgatgaaag aagcgggcat
    21481 caatgttgat tacgttctgg aattcgacgt accggacgaa ctgattgttg atcgtatcgt
    21541 aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac gttaaattca atccgccgaa
    21601 agtagaaggc aaagacgacg ttaccggtga agaactgact acccgtaaag acgatcagga
    21661 agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg actgcaccgc tgatcggcta
    21721 ctactccaaa gaagcggaag cgggtaacac caaatacgcg aaagttgacg gtaccaagcc
    21781 ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc taatcaagcc gcagcagatc
    21841 tgctttcggg caggtctgct catccacctc aggcaatacc tctcacagca attagttctt
    21901 cttcctcact tttccgctac aattatcaac aagttgaatc gataagaggc ggtaatgcgt
    21961 cagactaaaa ccggtatcct gctggcaaac ctgggtacgc ccgatgcccc cacacctgaa
    22021 gcggtaaaac gctatctgaa acaattttta agcgacagac gcgtggttga tacctcacgg
    22081 ttgttatggt ggccgttgct gcgcggcgtg attttgccgc tgcgctcgcc gcgtgtggcg
    22141 aagctgtacg cctctgtctg gatggaagac ggctcgccgc tgatggttta cagccgccag
    22201 caacagcagg cgttggcaca acgtttaccg gatacgccag tggcgctggg catgagctac
    22261 ggctcgccat cactggaaag cgccgtcgat gaactcctgg cagagcatgt agatcatatt
    22321 gtggtgctgc cgctttatcc gcaattctcc tgttctacgg tcggtgcggt atgggatgaa
    22381 ctggcacgca ttctggcgcg caaacgtagc attccgggga tatcgtttat tcgtgattac
    22441 gccgataacc acgattacat taatgcactg gcgaacagcg tacgcgcttc ttttgccaaa
    22501 catggcgaac cggatctgct gctgctctct tatcatggca ttccccagcg ttatgcagat
    22561 gaaggcgatg attacccgca acgttgccgc acaacgactc gcgaactggc ttccgcactg
    22621 gggatggtac cggaaaaagt gatgatgacc tttcagtcgc gctttggccg ggaaccctgg
    22681 ctgatgcctt ataccgacga aacgctgaaa atgctcggag aaaaaggcgt aggtcatata
    22741 caggtgatgt gcccgggctt tgctgcggat tgtctggaga cgctggaaga gattgccgag
    22801 caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat atgaatatat tccagcgctt
    22861 aatgccacgc cggaacatat cgaaatgatg gctaatcttg ttgccgcgta tcgctaaagc
    22921 tgagcggtaa agaactgagc gccgtcgcga agagcctcgt cggcggtttt catcatccgt
    22981 gaataatgca aaaaggcatg cagcgtacct gggtagagtt tgaactcaca gggctgctga
    23041 tgcgccgcta acgtctggta aagcagacgg ctgtcatcca gcagcggatc gaactcctcc
    23101 ccggcaataa aacagggcgg aacttcgcga gtgagatcat tattaaacag acagtaatac
    23161 ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt catacatctg caaatcctgt
    23221 tgcgttaagc catcccagac accgcccaac agacgacgag tcacggaatc ccgtaatccg
    23281 taaagcccat accacagcaa aacacccgca attttaccgc aatcgatctg tttatcacgc
    23341 aaccacaacg cactggcgag cgccagcatg gcacctgcgg aatcaccggc aaagccaatg
    23401 cgggacatat tgatttgata atcctccgcc tgctggtgga aataacaaca agcagccaca
    23461 atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa gggtgtaatc aataccaatc
    23521 accgtacatt ggctgtagct tgccagcagg cgcatgatgc gatcgtgggt atcgagattg
    23581 ccgagaataa aaccgccttc catgcaaata aaatagcgtc gccgggctat ctggctgcgg
    23641 acaaaagaga cgtgtttcca cctgcccata ttttgttgga accatgtaag ctctggttgc
    23701 catttctgga gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc
    23761 agcaatcgtt cccgttgcgg gccaggacgg taaatccggc tgaagagtat tcacaacggt
    23821 cttcatttca gcagaaataa ggtccagaac aggtagtttg ttttccggct tcatacaaaa
    23881 actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc
    23941 tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct
    24001 cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaaaatta
    24061 cttccctgta aatgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag
    24121 cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat attgaagcga aagtggatga
    24181 tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt
    24241 agccgaagtg ctttatcagg agctaaaaca gaaaaacctg attacccatc agtttgcggg
    24301 tggcaccatt ggtaacacca tgcacaacta ctcggtgctc gcggacgacc gttcggtgct
    24361 gctgggcgtc atgtgcagca atattgaaat tggcagttat gcctatcgtt acctgtgtaa
    24421 cacttccagc cgtaccgatc ttaactatct acaaggcgtg gatggtccga ttggtcgttg
    24481 ctttacgctg attggcgagt ccggggaacg tacctttgct atcagtccag gccacatgaa
    24541 ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg cactggttct
    24601 cacctcatat ctggtgcgtt gcaagccggg tgaacccatg ccggaagcaa ccatgaaagc
    24661 cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt
    24721 cattgccgag aatccgcagt ggtggcagca attcctcaaa gaccacgttt ctatccttgc
    24781 gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga
    24841 caaggcgctg gactgggtag atctggtgct gtgcaccgcc gggccaatcg gcttgtatat
    24901 ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggcgc
    24961 tatagcggaa ttcaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa
    25021 tccgctgcgt gtctattcgc acattgcgcc gtacatgggc gggccggaaa aaatcatgaa
    25081 cactaatgga gcgggggatg gcgcattggc agcgttgctg catgacatta ccgccaacag
    25141 ctaccatcgt agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc
    25201 atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca
    25261 ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag agtcttactg
    25321 ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc gttcacgccg catctggcat
    25381 ttggctctcg atgcctgatg cgacgctggc gcgtcttatc atgcctacat atttttcata
    25441 ttttacatcc ggcaaccacc gtttaccccg tcaccacttc acccgccggt ggcgtttcca
    25501 gcagttccag catagtacgg gcaatttcac gttcacccat cactacctga ttcgcgccac
    25561 gttcggtgat atacgtcact tcatcgtcat aatgggcgcg ggcgataatc tcaatatccg
    25621 ggtttttcgc gcgggcagac gccacaatct cacccgcttc ataaccgttg gggatcgtca
    25681 ggatcagcca ttttgcacat tccagatgtg ccagttgcat aatttcttcg ttcgctgcat
    25741 tgcccaatac agcgcggacc ccacgctctc gcagctcatc aacacgggtt cgtgacgtct
    25801 caatcaccac cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac
    25861 gaccgtaacc caccaatagc gcatggttgc aaatatccac tgggatctgt ttctcttctt
    25921 cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca
    25981 gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat cgcccctgcc agtaccaggt
    26041 tttgtccggc ctgcggcagt aaattcaatg ccattcccag tcccgccagg ataaacgcga
    26101 actcaccaat ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa
    26161 acagacggac aaggaacagg gccgcgaccg atttaccaaa cagaataatt gccagcgtcg
    26221 ccagcactgc cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgactgaga
    26281 caaaaaacag caccgcaaac gcgtcgcgca atggcaacgt atcgtgggcg gcacggtgac
    26341 tcaattcaga ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat
    26401 caaacagctc taccgcacca aaggcaatcc ctaacgccag cgccagcact gatagggtaa
    26461 acagttcgcg agaaccggtt gccgcgctgc gtgccataat ccacggcacc agacggcgac
    26521 ctaccagcat cataatggcg ataaatgcga tcaccttgcc gatggtgatc cccatatcga
    26581 ctgcaagagt ggcaaagccc acatcgccct gttccatcat tcctgccact gcgggcagca
    26641 acaccagcgt cagaaccatc accaggtctt ccacaatcaa ccaaccgatg gcgattttcc
    26701 cacgctgact gtctattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg
    26761 cggtggaaag acataagccg aacacgatac cggtcattaa cgaccagccc agcacggcgg
    26821 agagcgccat acccagcagc gtcgccacgg ctatctgggc gatcgcgccg ggaatggcga
    26881 tggactttac cgccatcaaa tccttgagcg aaaaatgcaa accgacgccg aacatcagca
    26941 aaataacgcc cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag
    27001 tgaatggtcc tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt
    27061 tattggccag catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa
    27121 gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggccg
    27181 ggaaaaccaa aattacaggt aatagtttat gacaatttca ttgatgatgt ttatgaataa
    27241 ttgttgaatt ttgcagaaaa atggaattag ctgcaaaaaa agcacggatc ggaaaacaga
    27301 aggcggttta acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc
    27361 tttggctgca atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc
    27421 ccaatagtgg caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga
    27481 taagccccag aactgccgct cccagacctc ccatgccgaa agcaaaaccg aaaaagagtc
    27541 cagaaatcat accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga
    27601 atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggcggg
    27661 cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt
    27721 tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca acggcaaaca
    27781 ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt tgcatcagat
    27841 aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa atgaggatta
    27901 acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt atcgtcgctt
    27961 tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt tgcgccaaca
    28021 ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg ccataaggcg
    28081 cgataatcac cgccgccagc aaggggccct gggaactgcc aaagttgccg ccgacctgaa
    28141 agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg cgagaagatt
    28201 ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga actgcgccaa
    28261 aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg ccaattggca
    28321 acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc agtagcgaag
    28381 aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga gaaaattctg
    28441 actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc agcagatgtg
    28501 agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt gacgcagccg
    28561 cgcccgccac aggctgggtt tgttcactca ttgccatagg aaagtcactt tttcagggtt
    28621 gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct gtatgcgtcg
    28681 tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga tttatcttca
    28741 ccggacgcag acttgtctat gatgtcgcgt catactattt tttaacacgt tgaaatcagg
    28801 tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg ttaaccacat
    28861 ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac aaaattacag
    28921 ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc gaatatggtc
    28981 tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct gaaggcggta
    29041 gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct gacttacagg
    29101 atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta
    29161 atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg gccaagttcc
    29221 cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt aaaccgtggg
    29281 cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc gatgacacag
    29341 caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc gccgatgaag
    29401 cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt atcgcggcga
    29461 cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc gatgtggaga
    29521 tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc
    29581 cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt acgccatgca
    29641 aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc aaatacgtgg
    29701 gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac cagctgattc
    29761 cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc gtgctttaca
    29821 ctcctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcaccgttc cagaacaaag
    29881 gcaaagcgca gctggaagtg aaaataggca aaaccaatgg tcgtctggaa ggcgatcgtg
    29941 acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc caaatggatc
    30001 gcactggtgc cgactttgcg gtgatgagcg gaggcggaat tcgtgattct atcgaagcag
    30061 gcgatatcag ctataaaaac gtgctgaaag tgcagccatt cggcaatgtg gtggtgtatg
    30121 ccgacatgac cggtaaagag gtgattgatt acctgaccgc cgtcgcgcag atgaagccag
    30181 attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac ggcaaactga
    30241 acgaccttaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt atggcgacat
    30301 taaacttcaa tgccaccggc ggtgatggat atccgcgcct tgataacaaa ccgggctatg
    30361 tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc
    30421 tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg
    30481 gatgtttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa tttggcatcg
    30541 aggatctttg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt gccgccggaa
    30601 acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtgggcag acgttttttc
    30661 tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc gaccatcgga
    30721 tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag atccagttga
    30781 cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat caccgttcac tgccaccagc
    30841 agcgttttgt agacctgatc cggattcaaa cctaattttt tgacgacttc atcgccaaaa
    30901 ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt gtttttttcg
    30961 agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag
    31021 catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg
    31081 gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata ataataaaaa
    31141 aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag cgcaacattt
    31201 gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca tatcgccccg
    31261 gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc agcacatcgt
    31321 acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcggc ggtgcattca
    31381 gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg gtcagcgtat
    31441 cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggcaatc tggttttcag
    31501 ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat agctggtaat
    31561 cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga agaaccatcg
    31621 cgatttgcca cagcggttgg gtagaaaaga gtgagggaga aatgcccatc tcctggctaa
    31681 tgtgctgcag gttttggagt tgctcctcgc taatgcgctc ttccagcgcc tcgcaggcag
    31741 gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg
    31801 cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg ctacccatat
    31861 gaatactgcc aatcagatga aaatggcgat tgccggggag ggtgatatcg atggcaggcc
    31921 aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac aacagatcca
    31981 tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg caaatggagt
    32041 tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct attcacaggg
    32101 aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgactacacc cggagatatt
    32161 cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc agagttgctg
    32221 catgttcatc gtaatcgcgt cagtgcactg atcaataaca atcgtaaact caccactgag
    32281 atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct aaacctccag
    32341 gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga attgggacgg
    32401 attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa ggtcgcgtaa
    32461 ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca tccggcatcc
    32521 gagcaccatt gcctgatgcg acgcttacgc gtcttatcag gcctacaaac ctgtgcctga
    32581 accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgc
    32641 gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta aaccgcagca
    32701 accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct ccggcaacta
    32761 ccgggttaag cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga
    32821 tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg
    32881 aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg
    32941 tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc tgggccagcg
    33001 ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcagac
    33061 gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca tcaattccag
    33121 cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat
    33181 atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac ggatcgcgta
    33241 cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt gctccttgcg
    33301 atgcctgagc tgaaatatcg gcttcaatcg ctttagtatc gacttgctga tcatttaaca
    33361 acgcctgatt acccagcaat aacgcatgac cttctgcttc accgctcacg cccagcccgc
    33421 gcaatgtgcg gaaaccgttg acctgcggta gctgcatatc gcctgcttta tcaaggatcg
    33481 ctcgtgccaa tggatggctg gaaccttgct ccagcgccgc cgccagacgc agcgcctgcg
    33541 cttcgtcaac atcagcaaat gttttcactg cgacaacctg cggcttccct tcggtcagcg
    33601 tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt
    33661 cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg
    33721 tcgccagtcc cagcgcacac gggcaggcga taatcagcac cgtagtggca atcaccaggg
    33781 tatagacaat ctgcggtgcc ggaccaaaga aataccagat tgccgcactg acaagcgcaa
    33841 taaccactac taccggcaca aatacggctg agattttatc cgccagctga ccgatttctg
    33901 gcttgctgct ctgggcctgg cgcaccatgc gaatgatcct tgatagcgtg gtatggctgc
    33961 caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg gcatggacgc
    34021 tatcgccttc gcctttttgc tgcgggattg gttcgcccgt cagcatcgct tcatccagcc
    34081 atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca
    34141 gcaacatacc tggctgcact tctgccagag gcacgctttt ttcaccttcg tcagtaacca
    34201 ggcgcgccgt cggtggggtt aaatcgagta atttctccag tgccttagaa gaacgctgac
    34261 gtgcgcgtgc ttccagcata tggccgagat tgatcagacc gataatcatc gcactggctt
    34321 catagtaaag atgtcgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg
    34381 aatagagcca cgccacgccc gttcccagcg ccaccagcgt atccatcgtc gctgcaccgt
    34441 tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc atcaccgcca
    34501 gggttatcag gccgataacc aaccacaggc tgcggttgtc ggcggtgacc atcatgttat
    34561 cgccgatcat cccccagacc atcaccggga tacccaccgc cagtgcgaca attgcctgcc
    34621 agcggaagcg cttcatcgta gcgacggcgg tttcttgctg gcgttcgcgg cgtttcgcgt
    34681 catcttcaat cgcttccgcg ccgtagcccg ctttttccac cgcctgcact aaatcttgtg
    34741 gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga
    34801 cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc
    34861 cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag
    34921 aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt
    34981 ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg
    35041 cagtcccggt aacgtgcgct tcagtgatag acacatccgc ttgttccaca tccgggcgct
    35101 gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccaggttca
    35161 ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag
    35221 gttaaacctt ccagaaaggg gaaggtcaag aaattaataa accaggcggg taaaagtccg
    35281 taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc tttgttttcc
    35341 tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc acaggggtga
    35401 ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt
    35461 acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg gacaaaatgc
    35521 cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg ctatcgtgac
    35581 ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac tggaatccat
    35641 ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg cggtacagga
    35701 caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca
    35761 tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct attgccacca ccagcctgat
    35821 taacgctgaa aacgccgaac aacgctggca gcgaatttta catatccaac agcaactggc
    35881 tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca
    35941 taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga
    36001 agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg aactggcaac
    36061 gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac gcgttcttca
    36121 ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc
    36181 cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct
    36241 ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg
    36301 caatagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt
    36361 gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc cctcggtcta
    36421 tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt cgcgctgctg
    36481 gggcaggctg cgttgctaat ggaagcctcg acctggggcg cctttgcttt tggcggtatt
    36541 gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc cagcaatggc
    36601 ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct agcgctctcg
    36661 ttgctatacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc ttttggcgct
    36721 tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt gctctacgcg
    36781 ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc ggtagggcgg
    36841 ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat tattgccggt
    36901 gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc cggtgcgttc
    36961 ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg
    37021 gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt ggcgattggc
    37081 gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt atcggcatta
    37141 gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct gctcgggcat
    37201 gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagg cattaacgcg
    37261 aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga actgccgaag
    37321 ctaatgaata aatccctgtg gcggcagagt acctggggca acatcatcgt cgtggtgttg
    37381 ataatgctga tgacggcggc gctaaattta gactcactcg ccagcgttgc cagcgccacc
    37441 tttttgatct gctatctggc ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac
    37501 gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat cgttggcttt
    37561 atctacagcc tgtggtccca gggcagccgt gcgttgatat ggattattgg tgcaatctta
    37621 ctcagcctta ttgtggcaat ggtcatgaag cgtaataaaa ccgtataaca cctctctgtg
    37681 cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc
    37741 ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat
    37801 cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct atgaagagaa
    37861 ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca cgcagcagca
    37921 tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc tggaagagag
    37981 cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg
    38041 cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat ccatgcgcga
    38101 ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact gcccgattat
    38161 cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag acggctcgga
    38221 tatgtagggt tatcccttct atcgcaatga cttcaacata cgtacctgca ccgagatcct
    38281 cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca
    38341 gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac
    38401 tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac agccaggcgg
    38461 cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc ggcaccagcc
    38521 agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa ccatttccgc
    38581 ccagcatctc ggctgccagc agcaacccgc cgagactgag ccagaaaata tgtggatgaa
    38641 cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca ccagctcggc
    38701 aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca ctactttgct
    38761 gttactggag gaaccgatct gctgtaacgc ttcggtgtat ttttgcgcta cgaagtagtt
    38821 caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg cgcgggcttc
    38881 tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc
    38941 cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg cctgacggat
    39001 cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat
    39061 tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat
    39121 cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga tgctgtcgcg
    39181 ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt tagtcatggt
    39241 caggttgatg atcgccagct ccagattgct gacttcatag gcagcgcgtg gcgcgtcaat
    39301 cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg agataacttc
    39361 ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa tgcgatccat
    39421 aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac caaaacgttc
    39481 taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc
    39541 gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt gtaccgtcca
    39601 taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa agagagttcc
    39661 cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat
    39721 gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg gtgatacgaa
    39781 gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc
    39841 ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca gcccaaccag
    39901 cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aaaccagaaa tctaccgcca
    39961 gcaagtctct tactgcgccc agacgccgac gctgtttggc gatacggtat acgataatct
    40021 gatctttccc tggcagatcc gtgaccagca gcctgacccg gccatttttc tcgattttct
    40081 cgaacgcttc gctttgccgg acagcatttt gacgaagaat atcgccgagc tatctggtgg
    40141 tgaaaaacaa cgcatctcat tgattcgtaa cctacaattt atgccgaagg ttttattgct
    40201 ggatgaaata accagtgcgc tggatgaaag taataaacat aacgtcaatg agatgatcca
    40261 tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata aagacgaaat
    40321 taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc aggaagcacg
    40381 ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg ctggtggtgg
    40441 tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt ctctggagcg
    40501 tcgggcgagc gataattcag ctgattattg tcggctatgt gctgaagtat attttcagcg
    40561 tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat gcggcgtgga
    40621 acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt attgccatta
    40681 cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt gaatttatcc
    40741 cgatgcaggt gatccctgtc gccgggatga ttgccggtaa cgccatggta gcggtggggt
    40801 tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt caggagaaac
    40861 tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt gattcgcgac agtattcgcg
    40921 cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt ttaccaggaa
    40981 tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa tatcagatta
    41041 tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc tgctatttaa
    41101 cctatcgtaa gttttataat tcgcgccacc agttggtggt gactcaattg aagaagaaat
    41161 gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc cgaacaacgg
    41221 aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt gcatcacccg
    41281 tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga
    41341 gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc ccaacctgat
    41401 gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc gccacctgct
    41461 gttgcaactg ttgaatttcc ggcttatcag ccgcctgctt cagcagttcg atttgcgcta
    41521 ccagcccctg gtagcgggtg tcctgatcct gcaacggaat ggttttcagc accgcttccg
    41581 catcttcaga acggttcagc gcaatcagcg tttctgccag cagcaggccg atctccccgt
    41641 tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg taattgcctt
    41701 cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc agcactttat
    41761 ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct accggttgcc
    41821 cgttctggaa cagatacacg gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct
    41881 gctccgcgtc gcagtccagc ttcgccagaa taaattgccc gttgtactgc gccgcgaggc
    41941 tttccaggat cggggttaac tgcaaacagt gctggctacg ttcagaccaa aaatagaaca
    42001 gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg ttaatgttga
    42061 caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt gacatggggg
    42121 cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc ccaggcagca
    42181 ggcgctcgag caccattact gcccaggtca ccagcgttac cggatagcgc atcttcggct
    42241 tctcgctaat aaaagcatgg cgcactttgt cgaccaccgc ttccggtccc aacgtaaagc
    42301 gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg acgttgtcgg
    42361 tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg ctgtggcgta
    42421 gctccatgcg cagcgcgtct gaccacgcct ccagcgcata tttactggcc gcgtaagcgc
    42481 cacgacccgg cgtggagatt aatcccatca ccgatgatgt catcacaata cgcccttcac
    42541 cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg
    42601 cggaaaactg ctgttccatc tgcgcacggc tgatggtgga aagggggcca tacatgccga
    42661 atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc acctcgtctg
    42721 ctgcgcgatt aacactttct ggtgaatcca gatcgatcaa cacgccggta aatcccatgt
    42781 tgttcatacg ctcaacatca ttcggttttc ggcaacctgc cagcacatga aaaccctggc
    42841 gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta attaagaccg
    42901 atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg atttactaaa
    42961 ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg gttgggatga
    43021 ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa aaagggcagc
    43081 agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa
    43141 cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc gttggcggct
    43201 ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc
    43261 aaaccatcat tgcgcccagt tcaaccagca cccaacgcgg ctgatgctgt ttgagcagag
    43321 ccggaaggcg cgccagccct tgttgcgagg tgtcgccgct gatgctggca ttaactaccg
    43381 acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg gcagacattc
    43441 gatatccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg gcggcacgga
    43501 aggttaacag gaccaggaac aggaagggca aatgccagcg gaaaacattg ttgaagttca
    43561 tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca ccggagttga
    43621 gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat cgggtaagtc
    43681 aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag tgagtctggt
    43741 gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg cgaagcacgt
    43801 cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg aaaacgtcga
    43861 gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg cgaaagcgtt
    43921 gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc tttccggcgg
    43981 tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg tgctgtttgc
    44041 cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg acctgctgtt
    44101 ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc tgcaactggc
    44161 ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg aagcatgatt
    44221 gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg gctggcgcta
    44281 agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat ggagaagggc
    44341 ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag ttcacgcgaa
    44401 gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg caagcagctg
    44461 actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt caaagcggtg
    44521 gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct gaaaccgcag
    44581 gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa aacggacgac
    44641 accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca ggaaccggat
    44701 tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc ggatgtcgat
    44761 aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt cggcggcaac
    44821 gagaaccagc tcgacggcta tgagaaatgg ttgttacctc agcttaaacc cgaacaacgc
    44881 tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg ctcgcaacag
    44941 ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt ggcggtagcg
    45001 atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac gctgggggca
    45061 gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct gacgctttca
    45121 gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt gctgctcaag
    45181 cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg ggcgcttggc
    45241 accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt gctggcaacg
    45301 cagcctttac gcgtattacg taatgatgtg gtcgcgaacg tctggccgct gaagttttat
    45361 ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg tggcagcatg
    45421 ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg tgtgctgggc
    45481 tggatgctgc tgaatgtact tcgccgcatg acgctgaaat cgctgcctct gcgcctggcg
    45541 gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc attttcgctc
    45601 tcctttatgc tgctggcact gctgctggtg ctgcgtggcg atctgctcga ccgctggcag
    45661 cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac agaacaggta
    45721 gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt ttatccggta
    45781 gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga agatgaggcg
    45841 cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa tccgattgtc
    45901 gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct ggcgaaacgc
    45961 ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga gttccgcgct
    46021 aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt ctattttatt
    46081 ttccctgaag gggcattaga cgggcaaccg cagagctggc ttaccagttt ccgctgggag
    46141 aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag cctgttagac
    46201 attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg ggcgctggag
    46261 gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca ggtgcaggtg
    46321 ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca cactcggtgc ggggaaaaaa
    46381 ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg ggtttgtttc cggcctggtg
    46441 gccgcaattg gtgcggaaac ggcactggca gtgttgcagg cgaaagtgtt tgatttcccg
    46501 tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcgct gctgctgtcg
    46561 cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt caggcagttt
    46621 gtggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc ctacgaagag
    46681 cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta cgccgcatct
    46741 ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt cggttttttt
    46801 aaaggccgaa ggaggtaaac cttcggcctt agctacgttt cctcagttat tcaataaact
    46861 gacggatcag ttccagcagc gcgattagcg ccttaagaac gatgagaact ttatcgagaa
    46921 aagttttcat ccgttgctcc cttaagggag aaagacaatc acagcttacc cttacgttgc
    46981 ctgtaactat gattttcttc atgtgctaga tgaggactgc ctctctccaa attagagcac
    47041 cagaatcagc accacctgta tcctgaccag gacttaaaag tacttttccg cactgcggta
    47101 atcccttgag gcagtatcac tactagcgtc gggattgtat cgccaacata gctgtttgaa
    47161 tatgtaggca tcctgactta taattcactt gctagatgag gactgtttaa tttctgcccg
    47221 gaacaaccaa gtcttgggcg gaaaaaataa aaaaaccgac tgtcatgtcg gttttttata
    47281 tctgtattct ggtgcaacca ttttgttctc acgacaatcg ccgtttcaga ataccgccca
    47341 tcactgcgcc ttaacccata ccgccacttc cgcccactca ccgcgaaaga caactttttc
    47401 cgcttttttc tcaagctgat agcgatacat tgggtcgtaa tattctttaa gtaacggcac
    47461 cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt tgcgttgtca gcgccgtatc
    47521 cagctgggca gcgagttcgt tatagcgctg taaccccagc cgacgcttaa tcgccgaaag
    47581 tccgtgatgc aggtattcgc aatattcctg ccagccctgt tcgtcgccat aagcgtgggt
    47641 aaaatcatga tgcatacgca agaaatactc ttcattcagg cgttcaagac ggatctcaaa
    47701 cggatcttct accaccgcaa tcgccgcctg agtcattcgc tcgcgcagac attccggcag
    47761 gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc cacaggcgca aatcctgacg
    47821 ggcgtcggtt tttagcattt cggcagccag caggttttca aaactcgcct ggctaagttg
    47881 tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc gccagccctt ccagatcaac
    47941 accgtttggc tgttgttgca ctaatagcgt tttaccgctg ccggtacaac cgccaatcag
    48001 cactatcggt ttttgtgcca gttcaatggt cgcctgaatc gcggtctggc gcagtgcctt
    48061 ataaccgcct tccaccagcg gataatcaat ccccgcttca tgcagccagc gttgcacaat
    48121 atgtgaacgt tgaccgccac gggcgcagca gagaatacct tgcggatttt gcaggcacgc
    48181 tgcccgccag gcgtacatac gctgctgacg aatttcaccc gccaccagtt tatgccccag
    48241 cgccagcgct gcgtctgagc cttgctgttt atagcaggtg ccaacggcgg cgcgttcatc
    48301 gttattcatt aacggcagat tgatagcggc gggcattgcg ccttgctcaa actcgatagg
    48361 ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg gcacgatagt cctgttccgt
    48421 gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc ttgcgcgcta ttttacgcgc
    48481 caacgcgcaa ggaaacttga tttttaactg cgtgggttgc cgaaaatttc taaaaatccg
    48541 ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat cttctaccgc tttgccgctg
    48601 ccaaatttgc gccatgccag actcaatggc gaaggagggc gcatcgttgg gattacccgg
    48661 ctgaccagtt gttgattatc gatcattgac tggcaaagtg attttggcaa aaaaccaatg
    48721 ccaacacccg ccagatgggc ggcgattttc gtttccatat cgggaacaat aatctctttt
    48781 tgccccggca atcgccaggc gacgcgtttg gttaaggtgc gggcgctgtc ttcaatattg
    48841 accgccggaa agcgacgcaa ctgcgcttct gttagcggct cttcaacgtt cgccagcgga
    48901 tgatccgccg ccatgacaaa tcgccattgc accgatccta agggatcaag actaaaggta
    48961 tttgccagcg cctcagttcc cgtgaccccg atagccagcg aaaaaccttc gtacaatagc
    49021 gagtcccaga cgcccatata gatttgtcgg gagatgtgaa actgggtaaa ggggtaacgc
    49081 tcattcagcc acgccagcaa ctgggcgacg gcctgggggt tatagagcag gttgttgatg
    49141 acaatattca cctggcgttc cacgccatca ttcacctgtt gcagctcgct gggcatactt
    49201 tccagccagc tcagccagtc tctggcctgg gaaagtagat gctcgccagc cgctgtcaac
    49261 gttacgctac gagtcgtacg gaaaaacagc gctactccgg tattctcttc cagaagttta
    49321 atgcgataac tgatcgtcgc cgtggtttta cataatcgtt ctgccgcttt tgaaaaactt
    49381 cctgtttcag caaccgcaat gaaagtccgc aaggtttctg gatcgaacat cttcaggtat
    49441 ccccttttaa atccgcaagt tgcgtgattt tcttatcctc tgatttatca gtatttttac
    49501 atgataatcc tgttcaattt gtggaccaaa tctagttttg gaaaaacatt ccaacttttg
    49561 tattgatgtt gttctcttaa ggttttagat tgcctgttat tgaaaccaag ctgaccggtc
    49621 ggcggtggtt gaacggaatt atgttacaag gacaaaaaga tgaaacttca ggtattaccg
    49681 ttaagtcagg aagcctttag tgcttatggc gacgtaatcg aaacgcagca acgggatttt
    49741 ttccatatta acaatggcct ggtggagcgt taccacgatt tggcgctggt tgagattctt
    49801 gagcaagacc gtacgcttat cagcattaac cgcgcgcaac cggcgaatct gccgctgacc
    49861 attcacgaac tcgaacgtca tccgctgggc actcaggcct ttatcccaat gaaaggtgaa
    49921 gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag acctgtcaac gctgcgggcg
    49981 tttatcacca acggtgaaca gggggtgaat taccatcgta acgtctggca tcacccactt
    50041 ttcgcctggc agcgcgtcac cgattttctg accatcgatc gcggcggcag tgacaactgt
    50101 gatgttgaaa gtattcctga acaggaactc tgttttgcgt gacgcctgca accgacttgc
    50161 ataagataaa ctaattgttc attgtttatg ctcacttgta ggtcggagtt aacgtaggta
    50221 tgacggaagt tagacggcgc ggcaggccag gacaggcgga gcctgtggca cagaagggcg
    50281 cacaggcgtt agagcgggga attgcgattc tgcaatatct ggaaaaaagt gggggaagtt
    50341 cgtcggttag cgatatttct ctcaatctgg atttgccgct ctccacgacc tttcgcttgc
    50401 tgaaggtttt acaagcagcg gattttgtct atcaggacag tcaattaggc tggtggcata
    50461 taggattagg tgtctttaac gtcggtgcgg cgtacatcca taaccgcgat gtcctctccg
    50521 tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg cgaaacggtc aatgtcgcga
    50581 tccgtaacgg caatgaagcg gtattaattg gtcagttaga gtgtaaatcg atggtcagga
    50641 tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc cggtgcgggc aaagcgctgc
    50701 tttatccgct ggcggaagag gagttgatga gcatcattct gcaaaccggt ttgcagcagt
    50761 ttacgccaac tacgcttgtg gatatgccca ccttgctgaa ggacctggaa caagcgcgtg
    50821 aactgggcta taccgtagat aaagaagagc atgttgtagg tctgaattgc atagcttcag
    50881 caatttacga tgatgtcggt agcgttgttg ccgctatctc catctccggg ccttcatcaa
    50941 gactgacaga agatcgtttt gtcagtcagg gtgagctggt cagagacacc gcccgcgata
    51001 tcagcacggc gttgggactg aaagcacatc catagtgtct gtcgcatccc gctctgcgga
    51061 gcgggttttt ttgacaaaat ttaaaagttg gaaaattttt ccaattaata gaggtaggaa
    51121 taaaatggca aaaatgagag ccgttgacgc ggcaatgtat gtgctggaga aagaaggtat
    51181 caccaccgcc ttcggcgttc cgggagctgc aattaatccg ttctactcag cgatgcgtaa
    51241 gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa ggtgtttcgc acatggcgga
    51301 aggttatacc cgcgcaacgg cagggaatat cggcgtatgt ctggggactt ccggtcctgc
    51361 gggcacggac atgatcaccg cgctcaattc cgcttctgct gattccattc ctattctgtg
    51421 cattaccggc caggcaccgc gcgcccgtct gcataaagaa gattttcagg ccgtagatat
    51481 tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca gttcgtgaag cggcgctggt
    51541 gcctcgcgtg ctgcaacagg catttcacct gatgcgttct ggtcgtccgg gtccggtact
    51601 ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag tttgatcctg acatgtacga
    51661 accgctgccg gtctacaaac ctgctgccag ccgtatgcag atcgaaaaag ctgtagaaat
    51721 gttaatccag gccgaacgtc cggtgattgt tgccgggggc ggggtaatca atgctgatgc
    51781 agctgcactg ttacaacagt ttgctgaact gaccagcgtt ccggtgatcc caacgctgat
    51841 gggctggggc tgtatcccgg acgatcatga actgatggcc gggatggtcg gtctgcaaac
    51901 cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac atggtgtttg gtatcggtaa
    51961 ccgttttgct aaccgtcata ccggttcggt agagaaatac accgaagggc gcaaaatcgt
    52021 tcatattgat attgagccga cgcaaattgg tcgcgtgctg tgtccggatc tcggcattgt
    52081 ctctgatgct aaagcggcgc tgacactgct ggttgaagtg gcgcaggaga tgcaaaaagc
    52141 gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc cagcagcgta aacgcacttt
    52201 gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg cagcgcgtgt atgaagagat
    52261 gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact attggtctgt cacaaatcgc
    52321 tgcggcacaa atgctgcatg tctttaaaga ccgccactgg atcaactgtg gtcaggctgg
    52381 tccgttaggc tggacgattc cggcggcact gggggtttgt gccgctgatc cgaaacgcaa
    52441 tgtggtggcg atttctggcg actttgactt ccagttcctg attgaagagt tagccgttgg
    52501 cgcgcagttc aacattccgt acatccatgt gctggtgaat aacgcctatc tcggcctgat
    52561 tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg caactcgctt tcgagaatat
    52621 caactccagt gaagtgaatg gctacggtgt tgaccacgta aaagtagcgg aaggtttagg
    52681 ttgtaaagct attcgggtct tcaaaccgga agatattgcg ccagcctttg aacaggcgaa
    52741 agccttaatg gcgcaatatc gggtaccggt agtagtggaa gttattctcg agcgtgtgac
    52801 caatatttcg atgggcagcg aactggataa cgtcatggaa tttgaagata tcgccgataa
    52861 cgcagcggat gcaccgactg aaacctgctt catgcactat gaataaggga gataaatgat
    52921 gttacgtttc tctgctaatt tatcgatgtt atttggtgaa tatgattttc tcgcccgttt
    52981 tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt atgtttcctt atgactacga
    53041 cattgaagaa ttaaaacagg tactggcgag taataaactc gaacatacgc tgcacaattt
    53101 accggcaggt gactgggcgg cgggcgagcg cggtattgcc tgtattcctg gccgtgaaga
    53161 agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAJTRQ010000014        52251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000014 DAJTRQ010000000
VERSION     DAJTRQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(13..912)
                     /gene="nhaR"
                     /locus_tag="OO332_000934"
     CDS             complement(13..912)
                     /gene="nhaR"
                     /locus_tag="OO332_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5941508.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(978..2144)
                     /gene="nhaA"
                     /locus_tag="OO332_000935"
     CDS             complement(978..2144)
                     /gene="nhaA"
                     /locus_tag="OO332_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5941509.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2730..2882
                     /gene="mokC"
                     /locus_tag="OO332_000936"
     CDS             2730..2882
                     /gene="mokC"
                     /locus_tag="OO332_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5941510.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(2986..4116)
                     /gene="dnaJ"
                     /locus_tag="OO332_000937"
     CDS             complement(2986..4116)
                     /gene="dnaJ"
                     /locus_tag="OO332_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5941511.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4205..6121)
                     /gene="dnaK"
                     /locus_tag="OO332_000938"
     CDS             complement(4205..6121)
                     /gene="dnaK"
                     /locus_tag="OO332_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5941512.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6498..6902
                     /locus_tag="OO332_000939"
     CDS             6498..6902
                     /locus_tag="OO332_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5941513.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6928..7641
                     /gene="msyB"
                     /locus_tag="OO332_000940"
     CDS             6928..7641
                     /gene="msyB"
                     /locus_tag="OO332_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5941514.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            7790..8356
                     /gene="satP"
                     /locus_tag="OO332_000941"
     CDS             7790..8356
                     /gene="satP"
                     /locus_tag="OO332_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5941515.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8391..8978)
                     /gene="mog"
                     /locus_tag="OO332_000942"
     CDS             complement(8391..8978)
                     /gene="mog"
                     /locus_tag="OO332_000942"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5941516.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9093..10046)
                     /gene="tal"
                     /locus_tag="OO332_000943"
     CDS             complement(9093..10046)
                     /gene="tal"
                     /locus_tag="OO332_000943"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5941517.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10325..11767
                     /locus_tag="OO332_000944"
                     /pseudo
     CDS             10325..11767
                     /locus_tag="OO332_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            11837..12613
                     /gene="yaaA"
                     /locus_tag="OO332_000945"
     CDS             11837..12613
                     /gene="yaaA"
                     /locus_tag="OO332_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5941518.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(12766..13062)
                     /locus_tag="OO332_000946"
     CDS             complement(12766..13062)
                     /locus_tag="OO332_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5941519.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13276..14562)
                     /gene="thrC"
                     /locus_tag="OO332_000947"
     CDS             complement(13276..14562)
                     /gene="thrC"
                     /locus_tag="OO332_000947"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5941520.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14563..15495)
                     /gene="thrB"
                     /locus_tag="OO332_000948"
     CDS             complement(14563..15495)
                     /gene="thrB"
                     /locus_tag="OO332_000948"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5941521.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15497..17959)
                     /gene="thrA"
                     /locus_tag="OO332_000949"
     CDS             complement(15497..17959)
                     /gene="thrA"
                     /locus_tag="OO332_000949"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5941522.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(17985..18104)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18040..18105)
                     /gene="thrL"
                     /locus_tag="OO332_000950"
     CDS             complement(18040..18105)
                     /gene="thrL"
                     /locus_tag="OO332_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5941523.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18319..19005)
                     /locus_tag="OO332_000951"
     CDS             complement(18319..19005)
                     /locus_tag="OO332_000951"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5941524.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19405..19545)
                     /locus_tag="OO332_000952"
     CDS             complement(19405..19545)
                     /locus_tag="OO332_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941525.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19641..20357
                     /gene="arcA"
                     /locus_tag="OO332_000953"
     CDS             19641..20357
                     /gene="arcA"
                     /locus_tag="OO332_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5941526.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20416..21768)
                     /gene="creD"
                     /locus_tag="OO332_000954"
     CDS             complement(20416..21768)
                     /gene="creD"
                     /locus_tag="OO332_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5941527.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(21826..23250)
                     /gene="creC"
                     /locus_tag="OO332_000955"
     CDS             complement(21826..23250)
                     /gene="creC"
                     /locus_tag="OO332_000955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5941528.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23250..23939)
                     /gene="creB"
                     /locus_tag="OO332_000956"
     CDS             complement(23250..23939)
                     /gene="creB"
                     /locus_tag="OO332_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5941529.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(23952..24425)
                     /gene="creA"
                     /locus_tag="OO332_000957"
     CDS             complement(23952..24425)
                     /gene="creA"
                     /locus_tag="OO332_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5941530.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24636..25505
                     /gene="robA"
                     /locus_tag="OO332_000958"
     CDS             24636..25505
                     /gene="robA"
                     /locus_tag="OO332_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5941531.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25502..26149)
                     /gene="gpmB"
                     /locus_tag="OO332_000959"
     CDS             complement(25502..26149)
                     /gene="gpmB"
                     /locus_tag="OO332_000959"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5941532.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26201..26722
                     /gene="yjjX"
                     /locus_tag="OO332_000960"
     CDS             26201..26722
                     /gene="yjjX"
                     /locus_tag="OO332_000960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5941533.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(26807..27133)
                     /gene="trpR"
                     /locus_tag="OO332_000961"
     CDS             complement(26807..27133)
                     /gene="trpR"
                     /locus_tag="OO332_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5941534.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27223..29160)
                     /gene="sltY"
                     /locus_tag="OO332_000962"
     CDS             complement(27223..29160)
                     /gene="sltY"
                     /locus_tag="OO332_000962"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5941535.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29371..31038
                     /gene="ettA"
                     /locus_tag="OO332_000963"
     CDS             29371..31038
                     /gene="ettA"
                     /locus_tag="OO332_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5941536.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31145..31312)
                     /locus_tag="OO332_000964"
     CDS             complement(31145..31312)
                     /locus_tag="OO332_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941537.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31346..32578)
                     /gene="nadR"
                     /locus_tag="OO332_000965"
     CDS             complement(31346..32578)
                     /gene="nadR"
                     /locus_tag="OO332_000965"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5941538.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32599..33981)
                     /gene="radA"
                     /locus_tag="OO332_000966"
     CDS             complement(32599..33981)
                     /gene="radA"
                     /locus_tag="OO332_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5941539.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34030..34998)
                     /gene="serB"
                     /locus_tag="OO332_000967"
     CDS             complement(34030..34998)
                     /gene="serB"
                     /locus_tag="OO332_000967"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5941540.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35104..35748
                     /locus_tag="OO332_000968"
     CDS             35104..35748
                     /locus_tag="OO332_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5941541.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            35776..36792
                     /gene="lplA"
                     /locus_tag="OO332_000969"
                     /pseudo
     CDS             35776..36792
                     /gene="lplA"
                     /locus_tag="OO332_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            36824..37087
                     /locus_tag="OO332_000970"
     CDS             36824..37087
                     /locus_tag="OO332_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5941542.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37248..37967)
                     /gene="deoD"
                     /locus_tag="OO332_000971"
     CDS             complement(37248..37967)
                     /gene="deoD"
                     /locus_tag="OO332_000971"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5941543.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38024..39247)
                     /gene="deoB"
                     /locus_tag="OO332_000972"
     CDS             complement(38024..39247)
                     /gene="deoB"
                     /locus_tag="OO332_000972"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5941544.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39299..40621)
                     /gene="deoA"
                     /locus_tag="OO332_000973"
     CDS             complement(39299..40621)
                     /gene="deoA"
                     /locus_tag="OO332_000973"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5941545.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(40748..41527)
                     /gene="deoC"
                     /locus_tag="OO332_000974"
     CDS             complement(40748..41527)
                     /gene="deoC"
                     /locus_tag="OO332_000974"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5941546.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            41785..43326
                     /locus_tag="OO332_000975"
     CDS             41785..43326
                     /locus_tag="OO332_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5941547.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43298..44161
                     /locus_tag="OO332_000976"
     CDS             43298..44161
                     /locus_tag="OO332_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5941548.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44375..45157)
                     /locus_tag="OO332_000977"
     CDS             complement(44375..45157)
                     /locus_tag="OO332_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5941549.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45154..46227)
                     /locus_tag="OO332_000978"
     CDS             complement(45154..46227)
                     /locus_tag="OO332_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5941550.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46349..46510)
                     /locus_tag="OO332_000979"
     CDS             complement(46349..46510)
                     /locus_tag="OO332_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5941551.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46637..47242)
                     /gene="osmY"
                     /locus_tag="OO332_000980"
     CDS             complement(46637..47242)
                     /gene="osmY"
                     /locus_tag="OO332_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5941552.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47635..49224)
                     /gene="prfC"
                     /locus_tag="OO332_000981"
     CDS             complement(47635..49224)
                     /gene="prfC"
                     /locus_tag="OO332_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5941553.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49315..49992)
                     /gene="yjjG"
                     /locus_tag="OO332_000982"
     CDS             complement(49315..49992)
                     /gene="yjjG"
                     /locus_tag="OO332_000982"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5941554.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50007..50453)
                     /gene="rimI"
                     /locus_tag="OO332_000983"
     CDS             complement(50007..50453)
                     /gene="rimI"
                     /locus_tag="OO332_000983"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5941555.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50422..50835)
                     /gene="holD"
                     /locus_tag="OO332_000984"
     CDS             complement(50422..50835)
                     /gene="holD"
                     /locus_tag="OO332_000984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5941556.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            50938..51969
                     /gene="rsmC"
                     /locus_tag="OO332_000985"
     CDS             50938..51969
                     /gene="rsmC"
                     /locus_tag="OO332_000985"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5941557.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12413 a        14163 c        13118 g        12557 t
ORIGIN      
        1 gggagctgcc gattaacgca ccgctggact aaaaagcgca gaataatccg tattgcagat
       61 tcgctgtacc gccgggtgct gaatcatccg ctcagcaaaa atagcatggt actcttccat
      121 cacattctcg acgcgaccaa tttctacgac agttttatcg gcataaaaat catatgcata
      181 aagcgttggg gcaacgaaga ttgcattgtg catcgcacca aaagctttca tcaaagcggc
      241 atcatcaaac tcgccgagga tttctacgtt taatccctgg gagttaaacc agttaagcaa
      301 tttgcgccct aacattgaac gtcgcccagg aatcaaaagt cgccgttctt ccagacaagc
      361 cgggaacggt ttttctggtg gtggatttgt acaccagaaa ctcacgccac attcgccaat
      421 tctcacggag aacaggcctt cctgctgcgt agagtctatc ggacagtcag aaatgatcat
      481 atccagttta tgctgactta attgctccag cagcatttcg tgggtggatt cgaagcagcg
      541 aagatgaatg ggctcgcctt ctaccactgc ggcgttaagt acgctactga ccaggcgttt
      601 ggaaagtgca tcagccacgc caacgtcaaa caataaattg gattctttgc gatagttcac
      661 aatatccagc atttcctggc ttaaggtgaa cattttatcg gcatagcgat agaccagttc
      721 tcccagctcg ctgggttcga gaccacgtcc cttgcgttta aataatttgc cttgcaggcg
      781 ctcttccagc gctcgaatct gtccggtaat ggtttgtgga gttaaataaa gcgcctccgc
      841 tgcgccaacc acggaacctt ctttatagac atgccagaag taatacaagt ggttgtaatt
      901 gatatgagac atgctcattt ctctccctga taacaatgaa aagggagccg tttatggctc
      961 cccggtaaac cgtcctgtca aactgatgga cgcaaacgaa cgcgtaacca gctgtatcca
     1021 attaccgccg aagagataga accgaccagg ataccgagtt tcgcccagtt aatcagttct
     1081 ggatctacgc taccaaaggc caggctggca ataaagatag acatagtaaa accgataccg
     1141 cacaggatcc ccaccaccat aatttgctga taagtcgttc cctcaggcag atgcgccagt
     1201 ttcaaacgca gcgccaacca gcagaacaga ctaatcccca gcggtttgcc aatcagcaag
     1261 ccagcgatga tccccaatgg cagaatggag gtcaagccat ccagcgtgac gccttgcagt
     1321 gaaacgccag cattagaaaa tgcaaacagc ggcaaaatca gatacgccac ccacgggtgc
     1381 aacacatgct ccagtcgctt cgctggagaa cgcccatgct tctctttcaa aggaataaag
     1441 aagccgacaa ttacccccgc cagagttgcg tgaacccccg atttcaacac cgcagtccac
     1501 aacaccacgc caacaagaat atagacgccc gtgcggcgta caccacacag attcaatacc
     1561 gcgagtaccg caattgctac agccgcgacg ccaagagagg ccatcgataa gtcattagtg
     1621 tagaacaatg cgatgataat gatggcccca agatcgtcga taatagccag agccatcaaa
     1681 aagatcttca gcgctaacgg aacacgactt cccaacagcg ccagtacacc aagtgcaaaa
     1741 gcaatgtcag tagccgccgg gatcgcccac ccttcgcggg taatcggatc ggcatagtta
     1801 aaaggcagat agagtaatgc cggcacaatc atcccaccaa tagcggcgat aactggaaat
     1861 gcggcctggc gtaagctggc tagcgatcct tgcatcagtt cacgtttaac ttccagaccg
     1921 actaacagga aaaataccgc catcagcgcg tcatttatcc ataacagcat gtttttgttg
     1981 atttcgagtg aaccaacccg gagctgaacc ggcgtctcca gaaagtcgtg ataccatcca
     2041 ctggttgcgc cgctgttggc cataatcatc gccaggatag cggcaatgat aagaataatg
     2101 cctcccgagg catcactgct aaagaattga tgcagatgtt tcacttttta tttctctttc
     2161 aggtgaatag atcgttttta ctattttacc ccgcacgaat catcgttaaa atcagattat
     2221 agtgtaggaa taattcgttt tttacgagct attgaagaat ttgcgccatc agcggtgaga
     2281 tgcgtttttg acggctgtaa gcgctcacga gtgaccctaa gcccagataa tcatagctta
     2341 aggtgaggat tcctgaggga ttaattagga aaagtaataa ttaatttgtt gtcagtcggc
     2401 gatgcttgtt aggccaatat ttcaaaaata atccagcttg tcgtcaataa ttgcgatgaa
     2461 gagataaatt aaagttaaat aattaaatca ttaaaaaatt acgccacaaa aatgcgaggc
     2521 gtcttcagga ttagaatcga taaaacaaaa tgggttaaag agagcgatat aatagcggcg
     2581 ggtgcttgag gctgtctgtc tcaggcatta gctgaacggc agatagagaa aagccccgag
     2641 tgatatttta ccatcaaccc gaggcctcct atatgctgaa cacatgtaga gtgcctctta
     2701 ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg attgtcgccc
     2761 tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt gacgagaaaa gacctctgtg
     2821 aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac gaatccgagt
     2881 aagagcaacg gcggggagtg atccccgcca ctttacaggt gctcgcatat cttcaacgca
     2941 ccctattttt acccaggcct gcccacgggc aggcttttgg ggaggttagc gggtcaggtc
     3001 gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt tgtgctcgcc
     3061 ggttgggcca ccgaagcttt cttgcagctc ttgcagcagc tgtttctgct tttcgttcag
     3121 gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcac caccgcggac
     3181 agacttgacg cctttaccgc gcatacggaa tagcttaccg gtctgggttt cgccaggcac
     3241 tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca gcgccgccat
     3301 agcgaagttg atcgggactt cgcaatacag gttgttgcct tcacgctcga aaatcgggtg
     3361 ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct cgcccgcttc
     3421 accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga ttttaacgga
     3481 cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg gatctttgat
     3541 cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaga atccctggcg
     3601 catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg tacctggttt
     3661 tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga tttctttggt
     3721 cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat cagcaccgcg
     3781 cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt caccaaaaat
     3841 atcgctgaag tctgcgccgc cgccaaaacc accgccgccc atgcctcctt gctcaaacgc
     3901 agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa cttcataagc
     3961 ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac ggtccgggtg
     4021 gtatttcatg gccaggcgtt tgtaggcctt tctgatttca cgctcttccg ctgttttgga
     4081 aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc ccctagatga
     4141 atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc cgtttataag
     4201 gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg tcatctttcg
     4261 cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc tgctgggcga
     4321 tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg atagcggctt
     4381 tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata gcagttttgt
     4441 cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggagctg tgcagcagat
     4501 ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct tcggcgttag
     4561 cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa gaagccttga
     4621 tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc aggataccgt
     4681 cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc gggttgatac
     4741 catctaggtt gaactgaccc agagatttgt tatcagccgc acgtttacgt tcaccctgca
     4801 gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc tggctgtgct
     4861 tggtcgggat agtggtgttt ttcgcgatca gcgtcgtcat cacaccgccc atggtttcga
     4921 tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca ccagtcagaa
     4981 caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg tctttacgcg
     5041 gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga gtctgaccac
     5101 caacgaggat aacgtcgtcg atatcagata cggacaggcc agcatcctgc agtgcaactt
     5161 tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt ttcgcacgag
     5221 tcactttgat gttcatgtgt ttcggaccgg tcgcgtctgc agtgatgtat ggcaggttaa
     5281 cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct tctttcaggc
     5341 gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg aattcttcaa
     5401 ccagatagtt gatcagacgg ctgtcgaagt cttcaccacc caggtgggta tcaccgttgg
     5461 ttgccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata atagaaatat
     5521 cgaaagtacc accccccagg tcataaaccg cgatagtacg gttgccagtg cctttgtcca
     5581 gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact tccagaccag
     5641 cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat gccggtacgg
     5701 tgataacagc ttcagttacc ggttcaccca ggtaatcttc agcggttttc ttcatttttt
     5761 tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact tcgacccatg
     5821 cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca cgctgtactt
     5881 cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg ttttgcgggt
     5941 tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc tgggtatagg
     6001 caatgataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga ggaatggtgc
     6061 catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt attttaccca
     6121 tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta ataggggcga
     6181 aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact gcggttgact
     6241 actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt ttgtgctttt
     6301 gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat tttattcata
     6361 taaagaatga atcgcagcca aggcattcat caaaaaattg taataaaaag aaaagattac
     6421 gtgcctgaat cttctcttta tcagcagtaa actagtgggt attcatcccc ctacctcttc
     6481 ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg gcgattagcc
     6541 tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc attgcaatgc
     6601 cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt gtgaaacgca
     6661 tacaactctc tgccgaccat ggcgatttac agttaagcgg tgcatcggtt tatttcaaag
     6721 ccgcccgtag cgccagtcag agcctgaata ttccttcaga aataaaagaa gggcaaacca
     6781 ctgactggat caacattaac agcgataacg acaataaacg ctgcgtctca aaaatcacct
     6841 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
     6901 aacgccagaa tatagaagcc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
     6961 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
     7021 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
     7081 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgccgg agaattacag
     7141 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
     7201 attttgctcg atgtttcaaa gcgattgaag ctgaaagccg acaaagagat gtctacgttt
     7261 gaaattgagc aacagttact ggaacaattt ctgcgtaata cctggaagaa aatggacgag
     7321 gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct ggaagagctg
     7381 ctgccgctgc tgatgaaaga caaattattg gcaaaaggtg tgtcgcattt gctttccagc
     7441 caactgaccc gcattttacg cacccacgca gcaatgagcg tacttgggca tggtttgctg
     7501 cgcggagcgg ggctgggagg ccctgtgggc gcagcattaa atggggttaa agcggtcagc
     7561 ggcagcgcct atcgcgtgac gattccagcc gtactgcaaa tcgcctgcct gcgccggatg
     7621 gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa ttttcatttt cccctcaaaa
     7681 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actacagtta
     7741 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     7801 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     7861 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     7921 ctacggcggc atcgcgcaaa tttttgctgg tctgctggag tacaaaaaag gcaacacttt
     7981 cggtttaacc gcattcacct cttacggttc tttctggctg acgctggttg cgattctgct
     8041 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     8101 tctgtggggc gtatttacgc tgtttatgtt cttcggcact ctgaaaggcg cacgcgtcct
     8161 gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg ctggcgatcg gtaacattgc
     8221 cggtaatgcc gcgatcatcc actttgccgg ctggattggt ctgatctgcg gtgccagcgc
     8281 aatctatctg gcgatgggtg aagtgctgaa cgagcagttt ggtcgcaccg ttctgccgat
     8341 tggtgaatcc cactaattgt cttgagatcc ccccgctcgg ggggattttt ttattcgcta
     8401 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     8461 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc atgcaccaca
     8521 acgttaccct cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     8581 ggtaagttaa ggatcagcgc ctgtttgcga atcacgccca cctgacgcga aaggatcgca
     8641 gttggtacaa aatgcaggct gatctggcgc atctgttcac caaagccagg catctcgcgg
     8701 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     8761 gtggtgagca ccagatggca actcatttca tccaccagct cacacaacgt ttgctcgatg
     8821 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     8881 gtcagccatt cttccagcgc tggaatgcct ttatcctgat aaacgccgct ggatgcgcga
     8941 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     9001 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     9061 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     9121 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     9181 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     9241 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctccgc cagctctttc
     9301 agcagtgccg gtgcgatggt cagacggtca cagcctgcca gttccagaat ttcgccgatg
     9361 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     9421 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcggta
     9481 ttcgctttgt accagtcaag aatacggcca acaaacggcg agatcaggaa cacgcccgcc
     9541 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatacct
     9601 tctttttcca gctgttctgc agcacggata ccctgccagg tagaagccag tttgatcaga
     9661 atacgatcgt tgctaatacc tgcatcgttg tagagtttga tcaggcgttt tgctttcgca
     9721 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acggcccgga
     9781 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     9841 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     9901 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     9961 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
    10021 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
    10081 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
    10141 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
    10201 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
    10261 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
    10321 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
    10381 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
    10441 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
    10501 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
    10561 cgttgcgctg gcaattaccg ccggtggacc aggtgccgtc ttttggatgt gggttgctgc
    10621 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
    10681 tgacgtcaat gggcagtttc gtggcggacc ggcatggtat atggcgcgcg ggctggggat
    10741 gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
    10801 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt tcttttgatt ttcctccgct
    10861 ggtgacgggc attattctcg ctgtctttgc tctgctggca atcactcgcg gtcttcatgg
    10921 cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg ataatctggg tactgaccag
    10981 cctggtgatt tgcgtaataa atattgggca acttccccac gtcatttggt ctatttttga
    11041 gagtgctttt ggctggcagg aagcggcagg cggcgcggcg ggatatacct taagccaggc
    11101 gattactaac ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg gatcgacgcc
    11161 aaacgcggca gcggcagcgg cagcggcagc ggcatcctgg cctccgcatc cggcagcaca
    11221 agggattgtc cagatgattg gcatttttat cgacaccctg gtcatctgta cggcaagcgc
    11281 catgctgata ttactggcgg gtaacggcac aacctacatg ccgctggaag gtattcagct
    11341 tatccagaag gcgatgcggg tgctaatggg ttcctagggt gctgaatttg ttaccctcgt
    11401 ggttattctg tttgccttca gctccatcgt tgccaactac atttatgccg aaaacaatct
    11461 cttcttttta cgcctgaaca accctaaagc gatctggtgt ttgcggatct gcaccttcgc
    11521 aacggtcatc ggcggcacct tgctaagcct tccgctgatg tggcaactgg cagatatcat
    11581 aatggcctgc atggctatta ccaatttgac cgccatttta ctgctctcgc ctgtggttca
    11641 taccattgcc agtgattatc tacgccagcg taaactcggc gtgcgcccgg tgtttgatcc
    11701 gttgcgttat ccggatatcg gtcgccagct ttctccggac gcgtgggatg atgtttcgca
    11761 ggagtaatca caactatcga tcaactcatt ctcttttttt gctaaagtcg gcataaattt
    11821 cctgcaagga ctggatatgc tgattcttat ttcacctgcg aaaacgcttg attaccaaag
    11881 cccgttgacc accacgcgct atacgctgcc ggagctgtta gacaattccc agcagttgat
    11941 ccatgaagcg cggaaactga cgcctccgca gattagcacg ctgatgcgca tcagcgacaa
    12001 actggcgggt atcaacgccg ctcgctttca tgactggcag ccagatttca cgccggcgaa
    12061 tgcccgccag gcgattctgg cgtttaaagg tgatgtctac accggcttgc aggccgaaac
    12121 cttcagcgaa gacgatttcg attttgccca acagcatttg cgaatgcttt ccggcttgta
    12181 tggcgtactg cgcccgctcg atttaatgca gccttatcgt ctggaaatgg ggatccgtct
    12241 tgagaatgcc cgagggaaag atctgtatca attctgggga gatatcatca ccaacaagct
    12301 gaacgaggcg ctcgcagcac aaggcgataa tgtggtgatt aatctggcgt cagatgagta
    12361 ttttaaatcg gtgaagccga agaaattgaa tgccgagatt atcaagccgg tgttcctcga
    12421 tgagaagaac ggcaaattta agatcatcag cttctacgct aagaaagcac gcggtctgat
    12481 gagtcgtttc attattgaaa atcggctgac caaaccagag caactgactg gttttaatag
    12541 cgaaggttac ttctttgatg aagattcctc cagcaatggc gaactggtgt ttaaacgcta
    12601 cgagcagcgt taagtttgta gtcaatgccg gatgcggcgt gaacgcctta tccggcctac
    12661 aaaagcatgc agattcaata tattgcagaa ttaacgtagg cctgataaga cgcgccagcg
    12721 tcgcatcagg cgctgaatgc cggattgtta cccggcattt gtcatttagc ggtgatgttt
    12781 gcctggacca tgaccgccgt gatgatcatg aggagctttc ttatggtggc gcggcggtgg
    12841 cggcggtccg tgtgggtgcc agcgattgcc tcgccattca taatgttgtt tccaccagcc
    12901 gtggtcgcgc cagtgacctc catcccagta atagccacga ttatcacgat cgcctatctg
    12961 taattttact gacgggacta acgtaatttc cgcagcctgt gctgccatgg gagcgaccag
    13021 aaccagggaa agtgcgagta cgatagattg catctttttc acaggttact ccttttatca
    13081 ttgcccgtta tgggtcgata tccggagatt acgcgatgag aacgacggtt gttattctcc
    13141 gcaatcctaa tctctaagtt accgcattta ttgataattt ctcctttttc cctgtcgttt
    13201 ttctccataa tttcttcata aaaaagccgg gctgcataaa agcaaacccg gcctgattga
    13261 gataatgaat agattttact gatgattcat catcaattta cgcaacgcag aaaaatcggc
    13321 gggcagatta tgtgaaagca agggtaaatc agcacgttct gccagctctt ttggcagatc
    13381 caacgtttca ccgagaatcg cttccacgct ctctttaaat ttcgccggat gcgcggtgcc
    13441 gaggaacaag ccatattcgc ctggattcaa ctggtcacgc agcgcacgat acgctacggc
    13501 agcgtgcggc tccgaggtgt agcccagttc ttttaactca cgcattgtct gttgcgtggt
    13561 ttcatcatcc acggctgcat aacccagctc tttcagttgc cagattttgc ggcggaacaa
    13621 ctcttccaca cgcggccagt tgttcggctg actcacgtcc atcgcgttgg ataacgtcgc
    13681 ctgagtcgct ttgggtgacc actgaccgtc gtgcaggaaa cgtggcacgg tatcgttcac
    13741 gttggtcgca gcaataaaac gtttcaccgg cagaccgaat gacttcgcca gcagacccgc
    13801 cgtcaaatcg ccgaagtttc cgcttggcac cgagacaacc agctggttgc gcgcctcctg
    13861 cggcagctgc gcaacagctt caaagtagta gcaaatctgc gccagcaaac ggctgatgtt
    13921 aatcgagtta gccgagttta accccagcgc cactttcagt tcttcatcat caaacgcctg
    13981 cttcaccagc gcctgacagg catcgaaatc gccgtcgatg gcaaccgttt cgatattgcc
    14041 gcccaacgta cagaacagtt tttcttgcag tggactgatt ttgcctcgtg gatagaggat
    14101 aaccactttc acattcggca aaccgtagaa agcatgagcc actgcagctc cggtatcacc
    14161 ggaggtcgcg gtcagaatgg tcactggctt atcatccgca atatgggtca gcatttgtgc
    14221 cataaagcga ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca attccagaca
    14281 accgacatcg ctttcaacat tggcgaccgg agccgggaag gcaaacgccg cgcgcacgcg
    14341 ctcttccagg atttcctgtg ggatttcatc accaataaac gccgagagga tcttcgcact
    14401 gcgggtgaca aaatccagct tcagcatctc atcaatttca gtcaggctga attccggcag
    14461 gtcgtgcgga aaaaacagcc cctgattttt gcccaacccc tgggttacgg cttgcgcaaa
    14521 gctgacctgc tcgttgtgat ctttcagatt gtagagtttc atttagtttt ccagtactcg
    14581 tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct tcctgatttt gcaggtagtt
    14641 cttacccaac cagtcggcaa cgcgctgggc ggtatccggc ttgtcacata gagcgaacaa
    14701 ggtcgggccg gagccggaga taccgctcgc taccgcgccg atttccgcga ccgcctgccg
    14761 cgcctgccgg aagccaggca gtaaccgttc acggtagggt tcagcgataa catctttcat
    14821 cagcttcgcg gcaagctcag gctgacggga atagcaggcg tgaatgaagc cagccagatg
    14881 tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag cccgggcttc
    14941 tgccgtcgag actttaattc ccggatacgc cagcacccac agccactcat caaaccctgg
    15001 cacttgctgg ctgatgatgt cgttttcttc gatcatcaac tgcataccac caagaaaaca
    15061 cggtgccacg ttgtcgtaat gaatgctgcc ggagatacgt ccttccagct cgcccatcaa
    15121 agccagcaaa cgagtgtcat taagcggctt gccgcagtgt tcattcatcg ccatcagcgc
    15181 cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg atcggcatat tcttttccag
    15241 agtcatcgcc actggaattt gcttgccaag ctcctggcaa aaacgctccc agcactgata
    15301 aacgatattt tcccgtggtt ctgacggcag cttatcggca aagcgtccga ggttgttgag
    15361 actgaatgtc tctgccgcct caaccgtgac tacatctccg agcaatgcac catcaacggg
    15421 tgccaccgcc gccccgagca catcaaaccc gacgctcata ttggcactgg aagccggggc
    15481 ataaacttta accatgtcag actcctaact tccatgagag ggtacgtagc agatcggcaa
    15541 agacaccggc agcggtaacg tcattgcccg caccatatcc gcgcagcacc aacggcagcg
    15601 gctgataata gtggctataa aaggccaggg cgttttcgcc atttttcact ttgaacagcg
    15661 gatcattccc atccacttcg gcaatcttca cgcggcagac accatcttca tcaatattgc
    15721 caacatagcg caaaactttt ccttcatcac gggccttcgc cacgcgcgcg gcaaagagat
    15781 cgtcgagctg tgacagattc gccataaaag cggcaacatc accctcggcg ttaaactctg
    15841 cgggcagcac aggttcaatt tcaatatccg ccagctccag ttcacgtccc gtttcacgag
    15901 cgagaatcaa tagtttacgc gccacatcca taccagaaag atcatctcgc gggtccggtt
    15961 cggtataacc catttcccgc gccagcgtgg tcgcctcgga gaaactcatg ccttcgtcta
    16021 acttgccgaa gatataagaa agcgaacctg aaagaatgcc ggagaacttc accaattcat
    16081 cacctgcatt gagcagattt tgcaggttct caataaccgg taatccagcc ccaacgttgg
    16141 tgtcatagag gaatttacgc cgcgattttt ccgccgcata acgcaactga tgatagtaat
    16201 ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct tcgcgcagga
    16261 agtcggcata ttgatccgcc actgcctggc tggaggtgca gtcaacaatg accgggttca
    16321 gcagatgata ttctttcacg aggcgaatta agcgcccgag attaaacggc tctttggctt
    16381 gtgcaagttc ttcctgccag ttttccagat taaggccatg cacactagtg agcagagcct
    16441 tcgagttggc aacgccgcag acacgtaagt cgatatgttt attcttcagc cagctttgct
    16501 gacgcttcag ttgctccagc agcgcaccgc caacgccacc gacgccaatc acaaacactt
    16561 cgataacctg atcggtattg aacagcatct gatgagtaac gcgcacgcca gtggtcgcat
    16621 catcgttatt taccacgaca gagattgagc gttcagaaga tccctgagca atggcgacaa
    16681 tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca cgcaaggtgc
    16741 gcataccatc acctaccacc gagataatgg ccagccgttc cgtcaccgcc agcggctcca
    16801 gtaagccttc tttcagttcc aggtagaact cttcctgcat tgcccgttca gctcgcacac
    16861 agtcgctttg tggaacgcag aaactgatac tgtattcgga agatgattgc gtaatcagca
    16921 ccacggaaat acgggcgcgt gacattgcag caaagacgcg cgccgccatg ccaaccatcc
    16981 ctttcatccc cgggccggaa acgctgaaca ttgccatgtt attcagattg gaaatgccct
    17041 tgaccggtaa ttcgtcttca tcacggctgg caccaatgag cgtacctggt gcttgaggat
    17101 ttccggtatt tttaatcagg caagggatct ggaactgggc gatgggggta atggtgcggg
    17161 ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc ttcctggtag gacatcgact
    17221 tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt atagaccccg tcaacgtccg
    17281 tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg gagtagtcgg
    17341 aaccgttacg tccaagcacc accagttcgc ctttttcatt accggcggtg aaacctgcca
    17401 tcaacaccat gtgatcagcc ggaatgcggc ttgccgcaat acggcgggtg gactcagcaa
    17461 tatcgacggt agattcgagg taatgcccca ctgccagcag tttttcgacc ggatcgataa
    17521 cggtaacgtt gtgaccacgc gcttctaaca cgccggccat aatggcgatc gacattttct
    17581 cgccacggca aatcagcgca gcgttgatgc tatccgggca ctgccccaac aaactaatgc
    17641 catgcaggac atgttttatt tgggcaaatt cctgatcgac gaaagttttc aattgcgcca
    17701 gcgggaaccc cggctgggcg gcggcgagtc ccgtcaaaag ttcggcaaaa atacgttcgg
    17761 catcgctgat attgggtaaa gcatcctggc cgctaatggt tttttcaatc atcgccacca
    17821 ggtggttggt gattttggcg ggggcagaga ggacggtggc cacctgcccc tgcctggcat
    17881 tgctttccag aatatcggca acacgcagaa aacgttctgc atttgccact gatgtaccgc
    17941 cgaacttcaa cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa aaagcccgca
    18001 ctgtcaggtg cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac cgttacctgt
    18061 ggtaatggtg atggtggtgg taatggtggt gctaatgcgt ttcatggatg ttgtgtactc
    18121 tgtaattttt atctgtctgt gcgctatgcc tatattggtt aaagtattta gtgacctaag
    18181 tcaataaaat tttaatttac tcacggcagg taaccagttc agaagctgct atcagacact
    18241 ctttttttaa tccacacaga gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa
    18301 tacttactaa ggcgtttttt atttggtgat atttttttca atatcatgca gcaaacggtg
    18361 caacattgcc gtgtctcgtt gctctaaaag ccccaggcgt tgctgtaacc agtcgaccag
    18421 ttttatgtca tctgccactg ccagagtcgt cagcaatgcc atgactcgtt cgcgtaaagc
    18481 ttgcagttga tgttggtctg ccgttgtgtc acttttcgcc ggttgttgta ttaatgttgc
    18541 taattgatag caatagacca tcaccgcctg ccccagattg agcgaaggat aatccgccac
    18601 catcggcaca ccagtaagca cgtcagccaa cgctaactct tcgttagtca gcccggaatc
    18661 ttcgcgacca aacaccagcg cggcatggct catccatgaa gatttttcct ctaacagcgg
    18721 caccagttct actggcgtgg cgtagtaatg atatttcgcc cgactacgcg cagtggtggc
    18781 gacagtgaaa tcgacatcgt gtaacgattc agccagtgtc gggaaaactt taatattatc
    18841 aataatatca ctagatccat gtgcgaccca gcgggtggct ggctccaggt gtgcctgact
    18901 atcgacaatc cgcagttcgc taaaacccat cgttttcatt gcccgcgccg ctgccccaat
    18961 attttctgct ctggcgggtg cgaccaggat aatcgttata cgcatattgc cactcttctt
    19021 gaccaaataa ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc gaatttacaa
    19081 attcttaacg taagccgcag aaaaagccct ttacttagct taaaaaaggc taaactattt
    19141 tctgactgca ctaacggttt aattgttaaa aaatgctaca tatccttctg tttacttagg
    19201 ataattttat aaaaaataaa tctcgacaat tggattcacc acgtttatta gttgtatgat
    19261 gcaactagtt ggattattaa aataatgtga cgaaagctag catttagata cgatgatttc
    19321 atcaaactgt taacgtgcta caattgaact tgatatatgt caacgaagcg tagttttatt
    19381 gggtgtccgg cccctcttag cctgttatgt tgctgttaaa atggttagga tgacagccgt
    19441 ttttgacact gtcgggtcct gagggaaagt acccacgacc aagctaatga tgttgttgac
    19501 gttgatggaa agtgcatcaa gaacgcaatt acgtacttta gtcatgttac gccgatcatg
    19561 ttaatttgca gcatgcatca ggcaggtcag ggacttttgt acttcctgtt tcgatttagt
    19621 tggcaattta ggtagcaaac atgcagaccc cgcacattct tatcgttgaa gacgagttgg
    19681 taacacgcaa cacgttgaaa agtattttcg aagcggaagg ctatgatgtt ttcgaagcga
    19741 cagatggcgc ggaaatgcat cagatcctct ctgaatatga catcaacctg gtgatcatgg
    19801 atatcaatct gccgggtaag aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga
    19861 atgttgcgtt gatgttcctg actggccgtg acaacgaagt cgataaaatt ctcggcctcg
    19921 aaatcggtgc agatgactac atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg
    19981 cacgcaacct gctgtcccgt accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg
    20041 ttgaaagcta caagttcaat ggttgggaac tggacatcaa cagccgttcg ttgatcggcc
    20101 ctgatggcga gcagtacaag ctgccgcgca gcgagttccg cgccatgctt cacttctgtg
    20161 aaaacccagg caaaattcag tctcgtgctg aactgctgaa gaaaatgacc ggccgtgagc
    20221 tgaaaccgca cgaccgtact gtagacgtga cgatccgccg tattcgtaaa catttcgaat
    20281 ctacgccgga tacgccggaa atcatcgcca ccattcacgg tgaaggttat cgcttctgcg
    20341 gtgatctgga agattaatcg gctttaccac cgtcaaaaaa acggcgcttt ttagcgccgt
    20401 ttttattttt caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact
    20461 ggctttcatt ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat
    20521 gccggccagc gccaccacca gcacactggt tcccaacaac agcgcgctat cggcagagtt
    20581 gagcagtccc cacatcactc catccagcaa caacagcgcg agggtaaaca acatgctatt
    20641 gcgccagcct ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact
    20701 ggcgattatc catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata
    20761 aaacatcacc aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt
    20821 gagcgtttca aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt
    20881 agtcgcccgg tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc
    20941 ggggaagttt tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc
    21001 aaaccagctg ctttgccatt gcgcctgaaa acctgactcg ctagcttccc gtttggctgg
    21061 tagaaaatca cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt
    21121 acgcccggca ggcaccacag aaagatcgcc ggtaccgctt aaattcagtg ccatattcag
    21181 ctgcaggttc tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc
    21241 ttgctctaac ccggtaccgg gttcaatggt cagtgccgtt ccgttaactt caggcgcttt
    21301 caccacacca ataccacgcg catccccgac gctaatcaca ataaagggct tgcctaagat
    21361 gatatttggc gcgttgagct cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa
    21421 atcactgtgc cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt
    21481 gccatcaacc atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac
    21541 ggttttatcc tcttcctgca ccgtataaag ctcggtcaca ggaatagcga taagcggccc
    21601 aacgagtttt tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta
    21661 atcggcacgt tcgacaatca cttgccgaat cagcattatc ggaatcaaca acagcaatac
    21721 tgcaccaaac aaggtggtca ttttccagaa caggggggat ttcaacatag tcttctcctt
    21781 tgcaatggca gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagttacg
    21841 gtgaagtcgt agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc
    21901 gttaaacaaa cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc
    21961 atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat
    22021 accactaccg gtatccagca ccttaagcgt gacgtgttcc tgatccactt cggcgcttag
    22081 cgttatgcaa ccgctctcgg gagtaaaatc gatggtgtta tccagtaaat tccccagcgc
    22141 ctgatccagt aacgccggtt cagcagcaac attaacctcg gtgggcataa catgcaaagt
    22201 gatgtttttt tctgccaact gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc
    22261 cacatcaaca gcagtcagaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa
    22321 cgtttccacc agcgcctgca ttcgcgcatt ttgtgtcaga atgttgtcag taaaacgagc
    22381 cactacttca ggcggcggac cttcgcgtaa aatttccgcc acgccacgaa tcgccgccag
    22441 tgggcttttt agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc
    22501 cagcttcacg cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag
    22561 atcgggaaga ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc
    22621 aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc accagtgcaa tccccaacaa
    22681 aatggcgctg gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc
    22741 gttcggtttg cctacgctca aaacgccaat aagccgcgag ccgcccataa tcggcgcggc
    22801 aacatacatc accgaacttt cgggatcggc aggattttgc aacgtgctgc gcgcaccata
    22861 ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa taatcctgtc caacggcttt
    22921 atttgccgaa tcgaacaata ctttgccatg cgcatcggtc atatagacat gatattcatt
    22981 gcgcactttg ttaatgccac caatattggc gcgaaacggg cgatgttgta gctgattaaa
    23041 cgcctgcgcc agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc
    23101 cgcaagtaac gttgccgtgt cgatcaacgt cccctccgtt gctcttcgca cgcccggttt
    23161 aacttctttg acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata
    23221 gcccagcagc aaccgcatgc cgatacgcat tacaagcccc tcaggctata tcccatgccg
    23281 cgatgagtat taatcggtga aagatcgggg ttgatggcgc gcagcttggc acgcagcgtt
    23341 ttaatgtgga tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc
    23401 attagttgct ggcgggacca gacgcggcct agcgacttga gtaacgtctt cagcaataaa
    23461 aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca
    23521 ttcaattcaa aatgaccaat acggatgacg ggagacggcg tcgagaactt cttcacccga
    23581 cgcagtaagg tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag
    23641 tcgtcagcgc caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg
    23701 aacagtacag gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccactg
    23761 atatcaggca aaccaacgtc gagaatcatg acgtcgggaa cctgctggcg agctttatcc
    23821 agcaccggca aacctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag
    23881 accagcgtgt cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata
    23941 aatcgcctct gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc
    24001 gaaccttcta caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca
    24061 taaaagcgca cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc
    24121 tcgccctgag ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga
    24181 caagaaatgg ccgcatcgga ggtatcttct gccaggccca atcccccttt aataccaccg
    24241 gttttcgccc ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc
    24301 acaacaattt tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct
    24361 tctgcatgag ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat
    24421 ttcatattgt taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca
    24481 tattgattaa gaacaaaatc tcaattcttt tatttccgtc aggccctaaa acatactcta
    24541 ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg
    24601 tcaggtgctc gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg
    24661 accttttaat ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg
    24721 cgaaagcagg ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg
    24781 ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga
    24841 ctgcgcgtcc gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta
    24901 cccgcgcatt caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat
    24961 ggagcgcctt tggtattcgc ccgccgctac gtctgggtaa attcacaatg ccagagcaca
    25021 aatttgtcac cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc
    25081 tggagcaaat ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg
    25141 gcaacgcgcc gaccattccg ccggtgctct acggcctgaa tgaaacgcgt ccgagtcagg
    25201 ataaagacga cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg
    25261 gctatgtact gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct
    25321 atgaaggtct gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc
    25381 caatgctcaa tctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag
    25441 acgccaaagc gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc
    25501 gttaacgctg cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt
    25561 caacgaccca gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac
    25621 agttacgcag acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca
    25681 gacatcccag tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc
    25741 ggcaagattc cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag
    25801 gaatacgccc gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg
    25861 tcagagaatc gatatgtctt ttttccagca cacccatgtt taattcacgc aggcgagaat
    25921 caaagatgat gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc
    25981 ctaaatcgct actgataata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca
    26041 tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct
    26101 cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc
    26161 gtatactgct gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttgtctgtgc
    26221 gaccaccaat cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga
    26281 aggatcctgc catattgcat ccgttgccgt cgagagcggt gtaccggaac aaccctttgg
    26341 cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc
    26401 agaggctgat ttttgggtgg cgattgaagc tggcattgat ggcgacagca ctttcagttg
    26461 ggtggtgatt gaaaacgcca gccagcgcgg cgaggcgcgt tcggcaactt tgccgttacc
    26521 tgcggtgatt ctggaaaaag ttcgtgaagg cgaggcgctt ggtcccgtga tgtcgcgtta
    26581 taccggtatt gatgagattg gccgtaagga aggggcgatc ggcgtgttta ccgccgggaa
    26641 actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcacaa
    26701 tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg acattcggtg
    26761 cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa tcgcttttca
    26821 gcaacacctc ttccagccac tgacgcagct ccacgggcgc ggctttcagg ctgttagatc
    26881 cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc tgactcattt
    26941 cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg cgctcatctg
    27001 gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca ttcttaagca
    27061 ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct gaatagggtg
    27121 attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgac tcccccggtt
    27181 gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac gacgtcccca
    27241 ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa tagcggtagt aagcatcata
    27301 agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct cgacaaatgc
    27361 cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc caggtccggc
    27421 gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa catattgcag
    27481 gtaactggtg ccaatgttga tgttcgtttc cggatccagc aattgcccag ggctgctata
    27541 accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa tctgcatcag
    27601 gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct gacgggcaat
    27661 cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga aaagatcgtt
    27721 gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga tcgttgcctg
    27781 aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag cctgctctgt
    27841 ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtattat cgagattcca
    27901 gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggtgc tgtcaacatt
    27961 ctgcggcgct ttatcaatct tcagctcata ctcttcaccg atgcgttgtg ctgcaaccat
    28021 cgggtagaaa ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt cagcttcacg
    28081 tccgcgttcc agcaataaat ccgcctgcca gtaacgccat tcatctttct cttttgcttc
    28141 catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc caagcgccat
    28201 tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatggcgt catcgcgcca
    28261 tttcgcctgc tcatcggtga catcattgcc catcaaacgc caggcgacga tatcgcgcag
    28321 ctcctgaatc tcatcttcat taagctgctg cgcctgggca agcgacggga tcatcagccg
    28381 tgcattttcg gcatcctgcc gcgccacact ggcaaacgcc accgccgcca tttgacgggt
    28441 aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc gtattagggt tgttcgccag
    28501 tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg ccagcactgt
    28561 taccaggcct gtgttacccg ctttcatcgc cagacggata cgctctaaat acgccagcgg
    28621 atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt taggctggct
    28681 cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct gcccggtgtt
    28741 ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt ccgggctaaa
    28801 ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac gagattgcag
    28861 cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg tcaccgccgg
    28921 ttgattcatc aggtcatcgg tgatctggcg gtattccagg tagggataaa gcggataatc
    28981 cttcagtcca ggcatcattt gttcgaccac atccatctgt cgattatccc aggcctgctt
    29041 gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca cgctgctgac
    29101 cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg ctttttccac
    29161 aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca taccgcgtaa tagatttatg
    29221 ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt actgcgaagg
    29281 tgatcgacca actgggatta ggcggggaaa caggctacac ttcgcctttg aaaactcatc
    29341 atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt
    29401 gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc
    29461 cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtccac cctgctgcgc
    29521 attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag
    29581 attggttatc tgccgcagga accgcagctg aacccggaac acaccgtgcg tgagtccatt
    29641 gaagaagcgg tttcagaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg
    29701 tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag
    29761 atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc ggcggatgcg
    29821 ctgcgtctgc cagattggga cgcgaaaatc gctaacctct ccggtggtga gcgtcgtcgc
    29881 gtggcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc
    29941 aaccacctgg atgccgaatc cgtggcgtgg ctggaacgct tcctgcacga cttcgaaggc
    30001 accgtggtgg cgattactca cgaccgttac ttcctcgata acgttgcagg ctggatcctc
    30061 gaactggacc gcggtgaagg tattccgtgg gaaggtaact actcctcctg gctggagcag
    30121 aaagatcagc gtctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag
    30181 aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg taaagcacgt
    30241 ctggcacgct ttgaagagct gaacagcacc gaataccaga aacgtaacga aaccaacgaa
    30301 ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt
    30361 aaatcctacg gcgatcgcct gctgattgat gacctgagct tctcgatccc gaaaggggcg
    30421 atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg tatgatctcc
    30481 ggtcaggaac agccggacag cggcaccatc actttgggtg aaacggtgaa actggcatcg
    30541 gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc
    30601 gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc
    30661 tttaacttta aaggggttga tcagggtaaa cgtgttggcg aactttccgg tggtgagcgc
    30721 ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa
    30781 ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc
    30841 ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac
    30901 atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt tactgagtac
    30961 gaagagtaca aaaaacgcac gctgggcgca gacgcgctgg agccgaagcg tatcaagtac
    31021 aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gcgaacgcct tatccggcct
    31081 acaaaaccag caatttcaat acgttgtaaa actgtaggcc tgataagacg cggtaagcgt
    31141 cgcatcaggc attgagcacc gaatgccgga tgcggcgtga acaccttttc cggtctacaa
    31201 cagccttagc atcctcaatt tcgtggcggg tcacccgtgc aggttttttc aaaccttgtt
    31261 taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaaactc attttatacc
    31321 tcgcctttga gcagtttcgt cacggttatc cttgctcccc catcatctcc cgcaccagct
    31381 ccacgcagcg caggaaacgg ctgtcgtaat cgtcctcttc aacccgcacg aattcgatat
    31441 tgttctcttc cagcatctcc accagcaagt tctggaactc tttgcgatcc accgaactgc
    31501 cgaggctgcg taaaccatcc gccacccacg gcgtgttgtt ctccagcagg atcaccagat
    31561 cgaaacggta ttcatcaatc agcgcctgca cgaacggatg ctcacgccct tcgtactttt
    31621 tgcagaacgc ctgggtagtg acaaaatcgg tgtcaataaa cgccacttta ttggcgtatt
    31681 tcactgcaaa atcaatgtat tgtgcgtggc ccaacgcgat tttatcgtag tcggaatact
    31741 gcaatgcgat ctcatcaccg ccgaggtgtg aaaagacata atcgcggcca tattcccacg
    31801 cactggtggt gttgaagata ttggcaagtt tgtttaccag ggtggattta ccgctcgact
    31861 cgccgccaag gatcgccacg gtacgcacaa aaaacggctt cacttcggta ggaatatatt
    31921 cccagtagcg gaacgggttt tcgcggatct gcgcaccgct gatactcata aaggtacgtt
    31981 tcggatcgac cagcaccgtc tcgatcccca gatgttccat atactgtggc gcatcggctt
    32041 cttccgaggt gtagatcagg tccggctgga tccccttttc agccataaac tttttgatgc
    32101 cgttgctcca cacatcccag ccatgcggat acggctccat gccctcttcg ttgaaagcat
    32161 gaatacggat atttttctga tatttaaagg tttgcaatag ccagcgcaga cgatccggca
    32221 ccgtgggctg ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg gtatcgtcaa
    32281 aacccataat gatatgcagc tcgtcaacct ggctacaggc gcgctggata aggtagatat
    32341 gtccggtatg cagcgggtag aacttaccga atacgacacc aatggttttc ttctgccggg
    32401 gaaattcaag ccccaaaaaa cggtgcaatg cctccagctt ttgcgcgctg gggcttttaa
    32461 ttttggcatt cagtaactgg cttaaatacc ctttggtcat accgctggca tcagctacct
    32521 gctgtagcgt gcagccctgt tgcttgatgg cagttttcag gtaatcaaat gacgacatat
    32581 ctccctccgt atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg agagtttttt
    32641 aacgccaaaa atctgcatcc cttccggcgc ttttttcggt acgttagccg ccggaacaat
    32701 cgcccggcga aaaccgtgtt tcgccgcttc agaaatccgc tcctggccac tgggcaccgg
    32761 gcggatctcc cctgccagcc cgacttcacc aaacaccacc agatcctgtg gcagcggtct
    32821 gtcacgcagg ctggaaacca tcgccagcag taacgctaag tcggcgctgg tttcggttac
    32881 cttcacgccg ccgaccacgt tcacaaacac atcctgatcg gccatttgca gaccaccgtg
    32941 acggtgcaag acagccagca ggattgccag acggttttgt tccagcccca ctgccacgcg
    33001 gcgcggattc gccattatcg agtgatcgac cagcgcctga atctccacca gcaacggacg
    33061 cgttccttcc cacaccacca tcacggagct gccggaggtc acttcatctc cgcgacttaa
    33121 gaaaattgcc gaagggttgc tgacttcacg cagcccctgt tcggtcatcg cgaagacgcc
    33181 cagctcattc accgcgccga agcggttttt atggctgcgt aaggtgcgaa aacgagagtc
    33241 ggcatcgcca tccagaagca ccgaacagtc gatacagtgt tccagcactt tcgggccagc
    33301 cagcgaacca tctttggtta cgtgtccaac catgacaatc gccacaccgc gcgttttggc
    33361 gaaacgcgtc agataagccg ccgtttcacg cacctgcgcc acgctaccag gcgatgattg
    33421 tacatccgcc atatgcatca cctggatcga gtcaattacc atcagcttcg gttgctcttc
    33481 ttcggcaatc aggcagatct gctcgatgct ggtttccgac aacatattga gattgtcagt
    33541 cggcaggcca aggcgatgag cgcgcattgc cacctgttgc agcgactctt cgccggtgac
    33601 atacagcgtt ttcatctgct gggccagttt gcacagcgtt tgcagcaaca gcgtggattt
    33661 ccccgcacca gggttaccgc caatcagaat ggcacttcct ggcaccacgc cgccgcctag
    33721 tacgcggtcg aactctttaa atccggtgga aaaacgcggc agctcttcaa ggctgatatc
    33781 ggagagtttc tggacttttg ccaccccggc gctaccggca tagccgctga gacgctcgtt
    33841 acgcgccacc gttggcgacg cagcaagacg cacctcggtg atggtgttcc aggcatgaca
    33901 ggcactgcac tgcccctgcc agcgcggata atcggccccg cattcattac aaacaaaggc
    33961 gcgttttgga gcttttgcca cggtgtacct cgttaatgct gtgccgcccg caggatgacg
    34021 ggcaggcaat tacttctgat tcaggctgcc agagaggatg cagaataccc ccatcaggtc
    34081 agcgtgacgg atggtgactt ccgccttttc attcactttt ggcttggcat ggtaggcaat
    34141 ccccagccct gctgctttga tcatcggcag gtcattagct ccatcgccaa tcgccacggt
    34201 ctgcgccagc gggatttcat actcctgcgc gaggcgagtc agagttttcg ctttgtactg
    34261 tgcgtctacg atgtcgccga tcacattgcc ggtaaattta ccgtccatga tctccagttc
    34321 attggctacc acggctgtca ggcgcagctt gtcgcgcagg tattcagcaa agaaagtaaa
    34381 gccgccggag gcaatcgcca ctttccagcc cagcgtttcc agcttgagca ccagctgcgt
    34441 taagcctggc atcagcggca gattttcacg cacctgttgc agaatattgg cgtcagcgcc
    34501 tttcagcgtc gccacgcggc tgcgcaggct ggcggtaaaa tcgagttcgc cgcgcatcgc
    34561 ccgttcggtt acctccgcta ccatctcgcc cgttccggcc agcttggcaa tttcatcaat
    34621 acattcaatc tggatggcgg tggaatccat gtccatcacc agcaaacccg gcgtgcgcag
    34681 gtgcgggatt ttccccagcg gggcgacatc cagctgcgct tcgtgggcca ggcgtgtagc
    34741 ccgtgcggtg agtgaacctg ccagacgaat cacctgataa tcttccacgc accaggcggc
    34801 aacaatcacc atcgccgcac ccagtttgct ctggtattgg gtcagacgct gtttatccag
    34861 cccacgacca tacagcagcc agccgctacg agctgcgtgg taatccagtg gcatcacttc
    34921 atcaccactt aatgaaagag gcagacccgg ccataaagag acatcttcag gcaggtcgca
    34981 ccaggtaatg ttaggcatta aggctcctgt aaaatcgttc gaagcaggga aaataacgca
    35041 tgaggctacc ttgtatccat tgcttctggc aacattaagt ctcaaatttt caaagggtgg
    35101 aagatggctc gcacaaaact gaaattccgg ctacatcggg cagtgattgt cctgttctgt
    35161 cttgccttgt tagtggcact gatgcaggga gcgtcatggt ttagtcaaaa ccaccagcga
    35221 cagcgtaatc cacagctgga agaactggcc cgcaccctgg cgcgtcaggt gacgctgaac
    35281 gttgcaccgc tgatgcgtac cgactcaccg gatgaaaaac gcattcaggc gatcctcgat
    35341 cagttaacgg atgaaagccg tatcctcgac gcgggtgtgt atgacgaaca aggcgatctt
    35401 atcgcacgtt ctggcgaaag cgtcgaagtg cgcgatcggc tggcgctcga cggtaaaaaa
    35461 gcaggcggct attttaacca gcagattgtc gagccaattg cgggtaaaaa cggaccgctc
    35521 ggctatctgc gcctgacact cgacacccat acgctcgcca ccgaagccca acaggtggat
    35581 aacaccacta acattttacg cctgatgttg ctgctctcac tggcaatcgg cgtagtgctg
    35641 acccgcacgc tgctacaggg taaacgcacc cgctggcagc aatcgccctt cctgttaacc
    35701 gccagcaaac cggtgccgga agaggaagaa agcgagaaaa aagagtgacc cattactaca
    35761 agaaaggaaa tcgttatgtc cacattacgc ctgctcatct ctgactctta cgacccgtgg
    35821 tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca gcgcgttctg
    35881 tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc gtggaaagag
    35941 tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag tagcggtggc
    36001 ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg caagccggag
    36061 tacgataaaa ctatctccac gtcgattgtg ctcaatgcgc tgaacgcgct cggcgtcagc
    36121 gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga ccgcaaagtc
    36181 tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac cttgctactc
    36241 aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa actggcggcg
    36301 aaaggcatta cttcagtgcg ttcccgcgtg accaacctca ccgagctgct gccggggctc
    36361 acccatgagc aggtttgcga ggccataacc gaggcctttt tcgcccatta tggcgagcac
    36421 gtggaagcgg aaatcatctc cccggacaaa acgccagact tgccaaactt cgccgaaacc
    36481 tttgcccgtc agagtagctg agagtggaac ttcggtcagg ctccggcatt ctcgcatctg
    36541 ctggatgaac gctttagctg gggcggcgtg gaactgcatt tcgacgttga aaaaggccat
    36601 atcacccgcg cccaggtatt taccgacagc ctcaaccccg cgccgctgga agccctcgcc
    36661 gggcgactgc aaggctgcct gtaccgcgcg gatatgctgc aacaagagtg cgaagcgctg
    36721 ttggttgact tcccggacca ggaaaaagag ctacgggagt tgtcgacgtg gatagcgggg
    36781 gcggtaaggt aactcgctga ttaaaatatc caaggaagga aatatgatcg atctggaaaa
    36841 ccaggaacgg gagattatca atttaatgtt cagccagcgt atttcctggc tagccgccgt
    36901 gcgtatcagg cataaattat cgcttgctga agtaagtaaa atgttaggta tctcgattaa
    36961 ttctctaaaa cagatcgaaa agacggaacg gcttagctca aacatcaaaa gcaaaatggc
    37021 agaaatttat ggttgtccac cagagttgct gatatgccct tcctggatga cggctgaaca
    37081 taaatgaata gattcgtgaa tagatacttg aatgtatttt attttctatc catatgaaaa
    37141 ataatagtaa atctataaat cagttgctat tgatgaatag atactaaaaa gccggagcag
    37201 tcccccactc cggcttaaca cgacaatcgc ctttcagcga aacacaatta ctctttatcg
    37261 cccagcagaa cggattccag tgcgattttg atcatgtcgt tgaaggtggt ctgacgctca
    37321 gcggcagtgg tctgctcgtg agtgcggatg tggtcagaca cggtgcagat ggtcagggct
    37381 ttcgcgccaa actctgcagc cacgccgtag ataccagccg cttccatttc cacgccgagg
    37441 atgccgtatt tttccatcac gtcgaacatt tcgccgtccg gagagtagaa caggtcagcg
    37501 gagaacaggt tacccacgcg agcatcaacg cccagtgctt tagctgcatc tactgcgtta
    37561 cgcaccatgt caaagtcagc gatagcggca aagtcatggt ctttaaaacg gatgcggtta
    37621 actttggaat cggtgcaggc ccccataccg ataacgacgt cgcgcagttt tacgtgcggc
    37681 agaactgcgc cacaggaacc aacgcggata attttcttca cgccgaaatc ggtgatcagt
    37741 tctttggtgt agatggagca ggacgggata cccataccgt gacccattac ggaaattttg
    37801 cggcctttgt aagtaccagt gaagcccagc ataccgcgaa cgttgttcac ttcacgggca
    37861 tcttcaagga aagtttcagc aatatacttc gcacgcagcg ggtcgcctgg catcaaaact
    37921 acgtcagcga aatcgcccat ttctgcatta atgtgtgggg tagccattgt tttatccttt
    37981 ttgtgacata acaaaggcgg agtcaacgct ccgcccaaat ccatcagaac atggctttgc
    38041 catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata tccgcgaagg
    38101 tttcacggtg acccagtgag cccggtttta ctttcgggcc atataccagt accggaatgt
    38161 gttcacgcgt gtggtcagta ccggtccagg tcggatcgca accgtggtca gcggtgagga
    38221 tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg tcgaacagtt
    38281 ccagacccgc agcataaccg gcgacgtcgc gacggtggcc ccaggaagag tcgaagtcaa
    38341 cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg atggtggcgt
    38401 caaacagcgc gtccaagcca gtcgctttca cttttttggt gataccgcag ttggcgtaga
    38461 tgtctgcaat tttaccgaca gaaaccacct ggccgtgttt ttcatcaacc agtttctgca
    38521 gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta cgctggaagt
    38581 taccagcttt gtcaccgata aacggacgag cgataacacg accgatattg tagccgccgt
    38641 tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaga ccgaaagttt
    38701 cttcatggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc ggcttgccgg
    38761 ttttcatgtg ctcttcgccc agttgatcca gaatgaccgt accggaagag tggcagttac
    38821 cgaggtaacc cggcagatta gcgcgttcga ccagtttatc cagcagctct tgcgggaagc
    38881 tgttttcgtg atcggagaaa tatccccact caaacagaac cgggacaccg gcgatttccc
    38941 agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg tacgcgccga
    39001 taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct tcgtgtgctt
    39061 tcgccagccc cagacggatc agatttggca gattgagcgg ccctttacga ccattatcag
    39121 cttcgccttt ggcacaagct tctgcgatat gacccagggt gtcagccccg acgtcaccaa
    39181 agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata ataaatgcac
    39241 gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca gtataccgtt
    39301 attcgctgat acggcgatag acagttggtg tgctttccgg tgcgttatcg gcaagtttaa
    39361 ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc gcgaggataa
    39421 ccgccagcgg acgctgaccg tctacctggt cgcccagacg cgccatatca gtaaagccga
    39481 cgctgtaatc gatggtgtca gatgcctgac gacgaccgcc gcccattgca accactgcca
    39541 tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca tagactgctt
    39601 tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg gtcgggcctt
    39661 tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc agcaccgcct
    39721 gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc agcatctcca
    39781 cgcacagcac catcgtgaca tcaaacagac gcgggttacg atactcaccc gtcaggaact
    39841 gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc tgattcatgt
    39901 cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca atcgcttcgg
    39961 caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact ttcacgtcca
    40021 tcaccagcgc gtccagacct tccgccagtt tcttcgccag gatcgaggca gtgatcagcg
    40081 ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta tcagccggag
    40141 ccagtgaact ggtctgaccg ataatcgcca cgccgacgtc tttaataatt tcgcggaaac
    40201 ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg agcgtaccgc
    40261 cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag gctgcgacca
    40321 tcggccccaa catcagcgaa gtgacatcgc cgacgccgcc ggtcgagtgt ttatcaacaa
    40381 tcgggccatc cagatgcagg cttttccagt cgagaacggt tcctgaatct cgcatcgcca
    40441 tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc atcgcgaggg
    40501 cggcaatctg cccttcggaa atagtgttgt cgcgaatacc gttgataaag aaacgaattt
    40561 cttcatcgct cagcgcatga ccatcacgtt ttttacgaat aatttcttgt gcgagaaaca
    40621 aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc tacgccttat
    40681 ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca ggcgtaaagc
    40741 atcttactta gtagctgctg gcgctcttac cgtcgccgtg gcccagcgct ttcagcaggc
    40801 ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atcagcccag tcagcaccga
    40861 acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc acgccgcccg
    40921 ccggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc atcatgatgc
    40981 gagcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg atgaagtccg
    41041 cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct ttcagttcgc
    41101 cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct ttacaggctt
    41161 tcaccaggtc aaaaccaacc tgctcgtcac ccgccatcag cgcgcggtac gggaatacca
    41221 cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc gcgatttcga
    41281 tgtcgtcgtt accgtgtggg aagttggtta ccgtagcgat acggatttcc ggggtgccct
    41341 gctctttcag agttttgcga gcaatcggga taaaacgagg atagatacag atagcggcgg
    41401 tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg tcggtgtcgt
    41461 cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt gctttcagat
    41521 cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt ttgttagtat
    41581 tctaacatct actccgcaac acacttcgat acacatcaca attaaggaaa tctacttaca
    41641 agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg aatgcgtcag
    41701 tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa tggcgcatca
    41761 ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact gcaacaacgt
    41821 tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca ttttctggca
    41881 ctggaagcag aaaacaatct gccttaccca cagcttcctg ccgaagcccg ccgcgcgctg
    41941 gatgaaggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc gcgctatgtc
    42001 ttacccgatt acgctcgttt tctggcgaac ggttccgaat ggctggagct ggaaggcgcg
    42061 aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt accgtcggtc
    42121 acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta tgttagaatt
    42181 ctaacacaaa acgagatcga tgttcgaata aaacgtttct ggcgttacct cgacagaacc
    42241 ctgccagatg cctttatgca cgccaatatc ggcccgtctg attcgcccat tacccgcgcg
    42301 atcttacgtg cagacgcgca ggtttcaccg aacctgacct ttatctacga tcctgaaatc
    42361 acccctgatg acctgctgct ggaagtggcg aagaacatct gtgaatgtag caaaccgcac
    42421 atcgccaacg gtccggtgca tgataaaatt ttcacaaaag ggggctacgg gattgtgagc
    42481 tgttacaact cactgccgct ggcgggtggt ggcagcacgc tggtacgcct taacctgaaa
    42541 gccattgccg agcgcagcga atcgctggat gacttcttta cgcgcactct accgcactac
    42601 tgccagcagc agatcgccat catcgatacg cggtgtgaat tcctctatca acaatcacac
    42661 ttctttgaga atagcttcct ggtgaaagaa gggctgatta accctgaacg ttttgtgcca
    42721 atgtttggca tgtatgggct ggcggaagcg gttaacttgc tgtgtgagaa agaagggatt
    42781 gccgcgcgct acggtaaaga agccgccgca aatgaagtgg gttatcgcat cagcgcgcaa
    42841 ctggcggagt ttgtcgccaa tacccccgtg aaatatggct ggcaaaaacg cgccatgtta
    42901 cacgcacagt cggggatcag ttccgatatc ggcaccacgc cgggcgcgcg tttgccgtat
    42961 ggcgatgagc cagatccgat cacccatctg caaactgtcg ccccgcatca tgcttattat
    43021 tattccggca tcagcgacat tctgacgctc gacgaaacca tcaaacgtaa cccgcaggca
    43081 ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc gtgaatttac cgccaatgtc
    43141 agcggtaacg atctggttcg cgttaccggt tatatggtgc gtttgtcgga tttagaaaaa
    43201 tatcgcgccg aaggttcacg caccaacacc acctggctgg gcgaagaagc cgcacgcaac
    43261 actcgtattc tggaacgcca gccgcgcgtg ataagccatg aacagcagat gcgctttagt
    43321 cagtaagatt atccccttct cctgcgttga cgggccaggc agtcgtctgg ctctgttttt
    43381 gcagggctgc aatctgcgct gcaaaaactg tcacaatccg tggacgatgg gacgttgcaa
    43441 tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg ttgcagattg ttgacggcaa
    43501 agtggtgtgg aacgctgtgg tctgcgagca atgtgatacc tgcctgaaga tgtgtccgca
    43561 acatgccaca ccaatggcgc aatccatgag cgtggacgaa gtgcttagcc atgtgcgcaa
    43621 agcggtgctg tttattgaag ggataacggt aagcggcggc gaagtcacga cccaactgcc
    43681 gtttgtagtg gcgctgttta ctgctatcaa aaacgatccg caactgcgcc atctcacctg
    43741 tctggtggac agtaacggta tgttgagcga aaccggctgg gaaaaattac tcccggtgtg
    43801 tgacggcgca atgctcgatc tcaaagcatg gggtagcgaa tgtcatcaac aattaaccgg
    43861 acgcgataat caacagatta agcgcagcat ctatttgctg gcagagcgcg gcaagctggc
    43921 ggaactgcgt ttgctgatga tcccaggcca ggtggattat ttgcaacaca tcgaagaact
    43981 ggcggcgttt atcaagggac ttggagatgt tccggttcgc ctgaacgcgt ttcatgccca
    44041 cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg ccggaagacg ttgagccgtt
    44101 ggctgatgca ttaaaggtgc gcggggtgag ccggttgata tttccggcgc tctatttgtg
    44161 acatgccgga tacggcctac ggttcggcac agacttgtag gcctgataag acgcgtcaag
    44221 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    44281 cggttcggca caaacctgta ggcctgataa gacgcgttaa gcgtcgcatc aggcattatg
    44341 cgccgactgc cggatgctgc ctacgaaggg caaactaccc acgaacgtta aacaccgcat
    44401 acgtgttatt aagcaacact tcggcaatct catccgcggg ttccgggcgt aactcgcaaa
    44461 gcacggcaaa cacacgggca gcctgttccg ggcggttagg ctgcccctga aaaccgttga
    44521 gcggcatatc cggcgcatcg gtttccagca acaacgatgc cagcggtaat tttgcgatga
    44581 catcgcgggt tttactggcg cgtggatagg tgatagtacc gcctacgccg attttgtagc
    44641 ccagctgtac aaaccgttca gcctgttgct ggctgccaga aaatccgtgg acaacaccag
    44701 cacgcggcaa atcgtggcgt ttaagatgca tcgccagttt gtcgtgcgtg cgccgtgaat
    44761 gcaggatcac cggcagatcg tagcgtttcg ccagtttcag ttgttcgtcg agtaaccact
    44821 gctgcctctc aaattgcgga tcgtcgccga agagatccag accgatctcc cccaccgcca
    44881 ccaccttcgc cggacgcctt tccagcgcct gctgtagctg ctcaagagac acatcgctat
    44941 gtttttccaa cataccagga tgcaagccca atgcggcata cagcggttga taattttccg
    45001 ctaacgcctg cacgcgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta
    45061 cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc gccaataaaa ggcgggaaat
    45121 caaaatggca gtgagtgtcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt
    45181 cgggtcgttc gcctgcggag cgtcgctcac ttcggcaacc agcgtatcgt tggcgactgg
    45241 cgcaggcgga atgacaatcg attccggcgt caccactggc accagatggc gggtaagcgg
    45301 cgctttttca gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc
    45361 aagtttatag tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgtaatgg
    45421 cttcggcgga taaatttcgg atatccgcag cttgcccggt ggtttctcaa taaattgctg
    45481 aatgtcacga tagctggttt catgatgctg caccagattg accagcggct gcaggctact
    45541 gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg
    45601 cacagtgcgg atgacgacca acgttttagc gccctgcctt gccgcctctt taaccggaat
    45661 cgcatcactg atcccgccat ccaggtagtt aatgccttcc agcgacactc cgctacgata
    45721 aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa
    45781 aaagtaattc ggcgcgtagt catcctgacg acaggcgcac atataaaacg atttgccgct
    45841 gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcgg tggcctccac
    45901 cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat caaaaaattc
    45961 gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa
    46021 cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg
    46081 cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc
    46141 gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc
    46201 aagcgttaca ggtattctct gccccacttc tgccttcctg tttttgttgt gaaagaaaga
    46261 caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt
    46321 ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca
    46381 ctaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg
    46441 ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc
    46501 aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa
    46561 atcactcaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag
    46621 gacgaattac gacgaattac ttagttttca gatcattttt aacgcttttc acaccatcta
    46681 ccgctttggc gatactttca gcacggtcac tttgtgcctg agaatctacg gtaccggaga
    46741 gctgaaccac gccatcggtg gtttcaactt tcactttacg tgaagggacg atatcgtccg
    46801 ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag tccttcaccg
    46861 agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacg ccttcaacgc
    46921 ctttcgccac tttcactgcc tcttcggcct gggcctgact ttcaacgaaa ccgctcaggg
    46981 tcacgacttt ttgatcggtt ttgacagaga tatcggtgct cttgatgttg tcatgatcca
    47041 ccagcgccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat
    47101 tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgcgcg ttgttttccg
    47161 cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa
    47221 tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagccta
    47281 gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt
    47341 cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagtctctga atcagttatt
    47401 aatatcggta gaatgacaat cgacggcttt agatactgat atctacgcat tgaacggtat
    47461 ttaacgccat cagaaatgtc atcactttgt taaatataga tcacaatttt gaaaccgctc
    47521 gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta
    47581 ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc cctggaggca agaattaatg
    47641 ctcgcgggtc tggtggaact gaacgtccgg ataacgttcc tgtgccaggc gcaggttgac
    47701 catgctggta gcgatgtaag cgaggttatc gccgccatca agcgccagtt ggctttcgtt
    47761 cttacgcttg aactcttcga atttcttcgc gtctgcacat tctacccagc gggcagtggc
    47821 aacgttgact gactcataca ctgcttcaac gttgtattcg ctcttcaggc gcgctaccac
    47881 cacatcaaac tgcagcacac caaccgcacc aacgatcaga tcgttgttgg agattggacg
    47941 gaacacctgc accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg
    48001 cttcagcgga tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc
    48061 ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt
    48121 gtgcaggccg aggatatcgc ccggatacgc ttcttcaacg tgcgaacggt cacccgccat
    48181 aaaggtcagc gcgtcggaga tcaccacatc tttcgcagtg cgcacctggc gcagtttcat
    48241 gcctttttca tatttaccgg acaccacacg cataaacgcc acgcggtcgc ggtgtttcgg
    48301 gtccatgttg gcctgaattt taaatacgaa gccggtaaat ttatcttcgc tcgcttctac
    48361 ggtacgggta tcggtctgac gcggcatcgg cgcaggcgcc cactccacca ggccattcag
    48421 catatgatca acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc
    48481 cgcaaggaac agctctttgt cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc
    48541 acgcagctgc tgtgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc
    48601 tttaacaatg cggacttcct gaatggtgtg ccctttaccg ctctgataga gataggtttc
    48661 atctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaattg gccaggtgat
    48721 cggtgcgcag ccaattttca gctcgttctc aacttcatcg agcagttcca tcgggtcgcg
    48781 gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac
    48841 ttccatcagc ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca
    48901 gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcgt gccccggggt
    48961 gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga
    49021 gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg
    49081 gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat
    49141 ggtagtttta ccggcgtccg ggtgagaaat aatggcaaaa gtgcggcgct tcgccacctc
    49201 ttgcaaataa ggagacaacg tcataattct ttcttcttga gtaagcggca tcgcgccgcg
    49261 cttgttgaaa acgaaaaatt gcggctattt tacccatcaa cgggggggag gcaatcagtg
    49321 tttacacagg agctgctcca gttcgtgcaa cgaagaaacg gtccaggtgg gcgcgatgcc
    49381 ttctggttgc tcgcgattgt gcgcattcag ccagcaggtc gcaagcccgg cgttgatgcc
    49441 accgagaata tcggactcgg cagtgtcgcc aaccatcagc acgcgtgaac ggtcaggatt
    49501 gcccgcctgt tccagcgcat aatcgaaaat tttcttattc ggtttggcaa cgccaacttc
    49561 ttcggaaatc accagcaaat cgaagtaatc acgcaggccc gtccgttcca gacgcacttg
    49621 ctgcaaggcg ctaaaaccgt tggtgatgat gccgatttta gcgttgccac gaatggcgtt
    49681 aagcagagaa accgcgcccg gcaacggcgt acagatttcc gccatcgcat taataaaggc
    49741 atcgttaagc aagcctgctt caactttcag ccgttcggcc cagctctcga aacgcccgtg
    49801 ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc acccacagtg gcttgttaac
    49861 ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg ctgtaatcaa gaaacatccg
    49921 ctgcaggccg gtgaatgagt caaaggtaaa cagcgtttca tcggcatcaa agaaaatcca
    49981 gtcccacttc attattccac cttgtcttac atactgattg gcaacgccat gatgatggcg
    50041 tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa tcgtcgcctc gttaaagcct
    50101 aaactttcgt acagggcaat ggcggcagca tttgaggcac ggacttccag ccatagtgtc
    50161 gccacgccgc gtttttccag ttcgtcgatc agatgttcca gcagcgccct acccagcccc
    50221 tgacgctgat agtcgggatc gaccgcaata ttgaacaatg tagcttcatc cagcaccact
    50281 tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta actgaaagtt gagataacgc
    50341 tcgccctggt tgctggcaaa ggttttttca ctccacggaa aggcgtgggc gcgttgttca
    50401 atgtggtaag ccgctggtaa atcagtcgtt tcgagggaag aaatcgtgtt catatgtgca
    50461 aatttgttgc cataacgcgg cgcgtgccgt tgggtttgcc cgtaattcgg tgagcgccgg
    50521 tgatgccacc tgagcgcctt ccaatgatag cggttcgtca gtacccaacc gccagctgtt
    50581 gcagcgactg ccttgcggca gcatcgcgat tttttctggc gtcagttgca gcacttgatc
    50641 ggggctgacg gttaatgcgc gcagaacatc gctcactaaa ggatcagtca gggcgggaag
    50701 atcgtttgcc accatcacca gacggacgtg tgccgggctc gcaatggcaa tctccccctg
    50761 caacgcgcca gggcgacgca gcgaccactg ggtaatgccc agttgctgta actgccagtc
    50821 tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg cgcaaatata gcaaattcgt
    50881 cgataccgcg ccaacatatg gctataatcg ccgccagtat caattgagga gcattccatg
    50941 tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca gtgatgattt cgaacaaagc
    51001 cgtattctgt ttgccggaga cttacaggat gacctgcccg cgcgtttaga taccgcggcc
    51061 agccgtgctc atacccagca attccaccat tggcaggtgt taagctgcca gatgggggat
    51121 aacgcccgtt ttagtctggt cgccacggcg aatgatgtcg cagattgcga tacgctgatt
    51181 tactactggc cgaagaacaa accggaagcc cagttccagt tgatgaattt actttctctg
    51241 ctgccagtgg ggacagatat ttttgtcgtt ggcgagaacc gcagcggcgt gcgcagcgct
    51301 gagcagatgc tggcagatta tgcgccgttg aataaagtcg acagcgctcg tcgctgtggc
    51361 ctctattttg gtcgtctgga aaaacagcca gtatttgatg cggataaatt ctggggcgaa
    51421 tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg
    51481 gatgtcggta gccagttgct gctctcgacg ttaactccgc acacgaaagg taaagtgctg
    51541 gatgtcggct gtggcgcggg tgtactttca gttgcctttg cgcgccattc gccgaaaatt
    51601 cgtctcacct tgtgcgatgt ctctgccccg gcggtagaag ccagccgcgc aacacttgca
    51661 gccaacggtg ttgaaggtga agtctttgcc agcaacgtct tttccgaggt gaaaggttgt
    51721 tttgatatga tcatctccaa cccgccgttc cacgatggga tgcaaaccag cctggatgcg
    51781 gcgcaaacgc tgattcgtgg cgcggtgcgt catcttaata gcggcggcga gctgcgaatt
    51841 gtagcgaacg ccttcctgcc ttacccggac gtgctggatg agacatttgg cttccatgaa
    51901 gttatcacgc aaaccgggcg cttcaaggta tatcgcgcca ttatgacccg ccaggcgaag
    51961 aaaggttgag tatctgatat ctgtcgccgc ataccgtgtc ggatgcggcg tgaacgcctt
    52021 atccgaccta caaaacctgc acgttagccc ttcgtaggcc agataagacg cgccagcgtc
    52081 gcatctggca tctgcccccg catgcctacc aaacctgcac gctagtttcc tgatggacat
    52141 ttttccagca attacacatc aggcgataat taactattga cgaaaagctg aaaaccacta
    52201 gaatgcgcct ccgtggtagc aattcttttt aagaattgat ggtatgcgaa g
//
LOCUS       DAJTRQ010000015        50136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000015 DAJTRQ010000000
VERSION     DAJTRQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..181
                     /locus_tag="OO332_000986"
     CDS             <1..181
                     /locus_tag="OO332_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941558.1"
                     /translation="PEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIG
                     MLGIAMICLLFIKDI"
     gene            292..1215
                     /locus_tag="OO332_000987"
     CDS             292..1215
                     /locus_tag="OO332_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5941559.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1219..2037)
                     /gene="nlpA"
                     /locus_tag="OO332_000988"
     CDS             complement(1219..2037)
                     /gene="nlpA"
                     /locus_tag="OO332_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5941560.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2259..2552
                     /locus_tag="OO332_000989"
     CDS             2259..2552
                     /locus_tag="OO332_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941561.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2593..3783)
                     /gene="nepI"
                     /locus_tag="OO332_000990"
     CDS             complement(2593..3783)
                     /gene="nepI"
                     /locus_tag="OO332_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5941562.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3994..4446)
                     /locus_tag="OO332_000991"
     CDS             complement(3994..4446)
                     /locus_tag="OO332_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5941563.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4499..5833)
                     /gene="adeQ"
                     /locus_tag="OO332_000992"
                     /gene_synonym="yicO"
     CDS             complement(4499..5833)
                     /gene="adeQ"
                     /locus_tag="OO332_000992"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5941564.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6008..7774
                     /gene="adeD"
                     /locus_tag="OO332_000993"
     CDS             6008..7774
                     /gene="adeD"
                     /locus_tag="OO332_000993"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5941565.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7820..9211)
                     /gene="uhpT"
                     /locus_tag="OO332_000994"
     CDS             complement(7820..9211)
                     /gene="uhpT"
                     /locus_tag="OO332_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5941566.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9349..10668)
                     /gene="uhpC"
                     /locus_tag="OO332_000995"
     CDS             complement(9349..10668)
                     /gene="uhpC"
                     /locus_tag="OO332_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5941567.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10678..12180)
                     /gene="uhpB"
                     /locus_tag="OO332_000996"
     CDS             complement(10678..12180)
                     /gene="uhpB"
                     /locus_tag="OO332_000996"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5941568.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12180..12770)
                     /gene="uhpA"
                     /locus_tag="OO332_000997"
     CDS             complement(12180..12770)
                     /gene="uhpA"
                     /locus_tag="OO332_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5941569.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12845..13135)
                     /gene="ilvN"
                     /locus_tag="OO332_000998"
     CDS             complement(12845..13135)
                     /gene="ilvN"
                     /locus_tag="OO332_000998"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5941570.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13139..14827)
                     /gene="ilvB"
                     /locus_tag="OO332_000999"
     CDS             complement(13139..14827)
                     /gene="ilvB"
                     /locus_tag="OO332_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5941571.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(14933..15031)
                     /gene="ivbL"
                     /locus_tag="OO332_001000"
     CDS             complement(14933..15031)
                     /gene="ivbL"
                     /locus_tag="OO332_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5941572.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15596..15685
                     /gene="tisB"
                     /locus_tag="OO332_001001"
     CDS             15596..15685
                     /gene="tisB"
                     /locus_tag="OO332_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5941573.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            15965..17149
                     /gene="emrD"
                     /locus_tag="OO332_001002"
     CDS             15965..17149
                     /gene="emrD"
                     /locus_tag="OO332_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5941574.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17157..17654)
                     /locus_tag="OO332_001003"
     CDS             complement(17157..17654)
                     /locus_tag="OO332_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5941575.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17651..18013)
                     /locus_tag="OO332_001004"
     CDS             complement(17651..18013)
                     /locus_tag="OO332_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5941576.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18003..18350)
                     /locus_tag="OO332_001005"
     CDS             complement(18003..18350)
                     /locus_tag="OO332_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5941577.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18459..18872
                     /locus_tag="OO332_001006"
     CDS             18459..18872
                     /locus_tag="OO332_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941578.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(18919..20302)
                     /locus_tag="OO332_001007"
                     /pseudo
     CDS             complement(18919..20302)
                     /locus_tag="OO332_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20337..22052)
                     /locus_tag="OO332_001008"
                     /pseudo
     CDS             complement(20337..22052)
                     /locus_tag="OO332_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22219..23112
                     /locus_tag="OO332_001009"
     CDS             22219..23112
                     /locus_tag="OO332_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5941579.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23109..23923)
                     /locus_tag="OO332_001010"
                     /pseudo
     CDS             complement(23109..23923)
                     /locus_tag="OO332_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23923..25539)
                     /locus_tag="OO332_001011"
                     /pseudo
     CDS             complement(23923..25539)
                     /locus_tag="OO332_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            25834..26508
                     /locus_tag="OO332_001012"
     CDS             25834..26508
                     /locus_tag="OO332_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5941580.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26547..28208)
                     /locus_tag="OO332_001013"
     CDS             complement(26547..28208)
                     /locus_tag="OO332_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5941581.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28404..28832)
                     /gene="ibpB"
                     /locus_tag="OO332_001014"
     CDS             complement(28404..28832)
                     /gene="ibpB"
                     /locus_tag="OO332_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5941582.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(28944..29357)
                     /gene="ibpA"
                     /locus_tag="OO332_001015"
     CDS             complement(28944..29357)
                     /gene="ibpA"
                     /locus_tag="OO332_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5941583.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29351..29423)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29662..29994
                     /locus_tag="OO332_001016"
     CDS             29662..29994
                     /locus_tag="OO332_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5941584.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(29996..31210)
                     /locus_tag="OO332_001017"
     CDS             complement(29996..31210)
                     /locus_tag="OO332_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5941585.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            31311..32329
                     /locus_tag="OO332_001018"
                     /pseudo
     CDS             31311..32329
                     /locus_tag="OO332_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32290..33627)
                     /locus_tag="OO332_001019"
     CDS             complement(32290..33627)
                     /locus_tag="OO332_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941586.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33702..34850)
                     /gene="dgoD"
                     /locus_tag="OO332_001020"
                     /pseudo
     CDS             complement(33702..34850)
                     /gene="dgoD"
                     /locus_tag="OO332_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(34847..35464)
                     /gene="dgoA"
                     /locus_tag="OO332_001021"
     CDS             complement(34847..35464)
                     /gene="dgoA"
                     /locus_tag="OO332_001021"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5941587.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35448..36326)
                     /gene="dgoK"
                     /locus_tag="OO332_001022"
     CDS             complement(35448..36326)
                     /gene="dgoK"
                     /locus_tag="OO332_001022"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5941588.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36323..37012)
                     /gene="dgoR"
                     /locus_tag="OO332_001023"
     CDS             complement(36323..37012)
                     /gene="dgoR"
                     /locus_tag="OO332_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5941589.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37290..37946
                     /locus_tag="OO332_001024"
     CDS             37290..37946
                     /locus_tag="OO332_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941590.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(37992..38804)
                     /gene="yidA"
                     /locus_tag="OO332_001025"
     CDS             complement(37992..38804)
                     /gene="yidA"
                     /locus_tag="OO332_001025"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5941591.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(38918..39316)
                     /locus_tag="OO332_001026"
     CDS             complement(38918..39316)
                     /locus_tag="OO332_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5941592.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39556..41970)
                     /gene="gyrB"
                     /locus_tag="OO332_001027"
     CDS             complement(39556..41970)
                     /gene="gyrB"
                     /locus_tag="OO332_001027"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5941593.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(41999..43072)
                     /gene="recF"
                     /locus_tag="OO332_001028"
     CDS             complement(41999..43072)
                     /gene="recF"
                     /locus_tag="OO332_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5941594.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43072..44172)
                     /gene="dnaN"
                     /locus_tag="OO332_001029"
     CDS             complement(43072..44172)
                     /gene="dnaN"
                     /locus_tag="OO332_001029"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5941595.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44177..45580)
                     /gene="dnaA"
                     /locus_tag="OO332_001030"
     CDS             complement(44177..45580)
                     /gene="dnaA"
                     /locus_tag="OO332_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5941596.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            45877..45957
                     /locus_tag="OO332_001031"
     CDS             45877..45957
                     /locus_tag="OO332_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941597.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46187..46327
                     /gene="rpmH"
                     /locus_tag="OO332_001032"
     CDS             46187..46327
                     /gene="rpmH"
                     /locus_tag="OO332_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5941598.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46344..46703
                     /gene="rnpA"
                     /locus_tag="OO332_001033"
     CDS             46344..46703
                     /gene="rnpA"
                     /locus_tag="OO332_001033"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5941599.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46667..46924
                     /gene="yidD"
                     /locus_tag="OO332_001034"
     CDS             46667..46924
                     /gene="yidD"
                     /locus_tag="OO332_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5941600.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            46927..48573
                     /gene="yidC"
                     /locus_tag="OO332_001035"
     CDS             46927..48573
                     /gene="yidC"
                     /locus_tag="OO332_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5941601.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48679..50043
                     /gene="mnmE"
                     /locus_tag="OO332_001036"
     CDS             48679..50043
                     /gene="mnmE"
                     /locus_tag="OO332_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5941602.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12379 a        13495 c        12642 g        11620 t
ORIGIN      
        1 gcctgaaaaa ataggctctg cgacaacgtt atatgcaaat acttcacgcg tcggctggat
       61 tatcgccggc tctgttgacg gaattatggt tgaaatctgg agctaccatg cgttgttctg
      121 gctggcgata gggatgttgg gtattgcgat gatttgcctg ctgtttatta aagatattta
      181 gctcaccagt ctgggctaag aaaaatcggc gtggcgcaaa cagagccatc ctcgccattt
      241 ttgctatcat gcctgcatac ataaacgaca aaacagtatg cagagggaaa aatgggttcc
      301 accagaaagg ggatgctgaa cgttctgatt gccgccgtgt tgtggggaag ttcaggggtc
      361 tgtgcgcaat acatcatgga gcaaagccag atgtcgtcgc agtttttgac tatgacgcgt
      421 ttgatattcg ccggtttgat tctgctgacg ctgtcatttg ttcatggcga taaaatcttt
      481 tctattatta acaatcacaa agatgccatt agtctgctga ttttttccgt ggttggcgcg
      541 ctaaccgtac agctcacttt tttgctaacc atcgaaaaat cgaacgcagc cacggcaacg
      601 gtgctgcaat tcctctcacc gacgattatc gtcgcctggt tctcactggt acgtaaatcg
      661 cgcccgggca ttctggtttt ctgcgctatt ttgacatcgc tggtcgggac ttttttattg
      721 gtgacacacg gtaatccgac gtcattatcg atctctcctg ccgcgttatt ctggggcatt
      781 gcctcggcat ttgctgctgc attctatacc acctatccct caacgctgat tgcccgctat
      841 ggcacgttgc ctgtcgtcgg ctggagtatg ctgatcggcg gtctgattct attgcctttt
      901 tatgccagac aagggacaaa ctttgtcgtt aacggcagtt tgattttggc gtttttttat
      961 ttgatcgtca ttggtacgtc cctgacattt agtctgtacc tgaaaggagc acaattaatt
     1021 ggcggtccaa aagccagcat tttgagctgt gcagaaccat taagtagcgc gctactctct
     1081 ttgctgttgc tggggatcac gttcacatta ccggactggc tgggaacgct gctgattctg
     1141 tcatcggtaa ttttgatttc aatggattcc cgtcgccgcg ccagaaaaat aaatcgtccg
     1201 gcgcgggatg agtgagaatt accagccagg caccgcgcca ccgttaaaga tggtttctgc
     1261 cgctttagcg acttcggatg attgataaga ttgcagaaat tccttcacgt tttctgcatt
     1321 cttattatct tcccgtgcca ccaaaatatt cacatacggc gaatttttat cttcaataaa
     1381 tacactgtcg tgcaccggag aaagcccggt ctgctgaatg taagtggtgc tgataatcgc
     1441 cacatcaact ttgggatcat ccagtacgcg cgggagctgc gccccctcga gttccataat
     1501 ctgcaaatga cgaggattat cggttatatc cagcgcggta ggtaataacc cttttccctc
     1561 tttcagagta attagcttct ctttttgcaa cagtaaaagc gcacgaccaa ggttggtcgg
     1621 gtcgttgggg atcgccactg tcgcgccttc ttttatttgc gccacggttt tgattttttt
     1681 ggaataaccc gccatgggga agacaaacgt atttcccacc gctaccagtt tataaccatg
     1741 cgcctgatta tcttgttcaa gaaagggtcg atgctggaag acgttcgcat caagttcgcc
     1801 atgatttgtc gcatcgttcg gtagcaacga accgctaaat cctaccagtt gaacatcgag
     1861 gccatatttc tcttttgcca cctttttagc gacttccgcg acatcttgtt ctgcgccatt
     1921 tataacgcca actttaatgt gttttgcatc gctgctactc tggtcgcaac ctgccagcag
     1981 aattccggcc agcaataatg cggcccctgt ccgtagatga tgtgttgtca gtttcatttt
     2041 tttatccttt tattaatgat tacctgacta tagcggtaag gtcgctgcgg tttaaaaaac
     2101 gaaacgctat cgataagaat aaaaaggaat aaaagtggaa tataaagtct ggcacttctt
     2161 actgaccacg caagcgcgtt tcgtacagca tgatgagagc gatgaaagca aactacactt
     2221 atgcttcatt cgatacacat ttgtaaaagg ttgaatccat gaaaccaacg acactactgc
     2281 ttattttcac ttttttcgct atgccaggca tcgtatacgc cgagtcgcct tttagctcac
     2341 tgcagtccgc aaaagagaaa accacggtat tacaagattt acgtaaaatt tgcacgccac
     2401 aggcgtcatt atcagatgaa gcgtgggaaa agttaatgtt gtcagatgaa aacaataaac
     2461 aacatatacg cgaggccatc gtggcgatgg aacgtaataa tcagagcaat tattgggaag
     2521 cgttgggtaa ggtagaatgc ccggatatgt aagaacagca acaactactg accaacgctt
     2581 ctttacctga tatcaggatt tcttcatttt cacctttgca gtaaccaaca atgcagtcag
     2641 caacatcaat gtgccggaca acatcaacgg cgaagtcaga ccaatattat ccagcgcata
     2701 accgccgatt gccgcgccac aggtattagc aagctgaata accgccacct gaatagaccc
     2761 ggctttttct gcctgatcgg ccagcgagcg ggtgatccac gttgaccagc cgacgggaac
     2821 caatgcaaaa gttagccccc agataatcgc cacgccggta gcaacgattt tatcgcttcc
     2881 ccacaacgtc agtaccaacg cactcacagc cagtattaac ggcgcgcctg ctaaggccag
     2941 ttttaccgaa cgtttaagaa tgaacgacga aagcgacgta ccaataaagc tggcgatacc
     3001 aaaactcaac agcaccagcg ttaagccatc cacgccgaat cccgccaggt tcatatacac
     3061 cgggcgaata tacgtgaaga aagcaaactg cccggcgaaa gacatgaaga tggcgatcat
     3121 ccctgccatc acacccggac gttgtaataa gcggaaagta ttttgtttct gatgcgaggg
     3181 ttcgcctggc agtgaaggca atgatttgat aatccagaaa atacacagca cgcccatcac
     3241 cgccgccgca ttaaagacat tgcgccaacc gataagctcg cctaaaaaac tgcccaacgg
     3301 cgcggcaatc accagcgcaa tagaaaccgc gccgaagatc accgacagcg ccttcggcac
     3361 cgtacgcggc ggtaccagac gcatggtcag cgacgccgac atcgcccaga acccgcccag
     3421 cgccagcccc agacaggcac gaccgattaa aagcaaactg aatgagttag caaaggaaac
     3481 cagcaagcag gagagcgtca gcaaaacggc aaacaaaata acaacgtagc ggcggtcagt
     3541 ggcctgaatt gtctgggtaa taaacaaact ggcaaacatt gccacaaagg cggtcacggt
     3601 caccgattgc ccggcaaccc cttccgaaat gcctaaatcc tgggccattg gcgtcaacaa
     3661 actgacgggc aaaaactcaa cgataatcag acaggcgaca caaaacgcca ccgagaaaac
     3721 ggctgaccag ttcggtcggg tgatggcatc cgcgccgcgg ttttcggcaa taaattcact
     3781 catggtgtta cccgtggcat gtttcagggg aaaagccgac cactttaaca ttgaaatgtg
     3841 tgacgcattt aacgtttttg caacttttac ggtgttgctt gagctggagt taagatcgaa
     3901 acggaacagg gcaaagatta ccggatgcgg cgtgaacgtc ttatccaccc gacaggtcag
     3961 gcgcggtctg taggctgata agacgaaacg gtttcaatgc aaccagtgaa cgccctcggc
     4021 aggctgaaag aacgcgttat agcgcatctg aaaagcatta atatcctgca tttcaggttc
     4081 ggtttcacgc agaaaaccca gcgccagcct gacctgagca tcggtaatcg gcgcggaaag
     4141 gcgggctttt tgtaaaatcc ggtgccattg cagcaggttc tgttcgctat catcgacaat
     4201 gacaatttgc cagataagca acacctgcgc ggcgttttgc aggtgcgact catccgggcg
     4261 atgcaaataa cgtaaaaatt cgtcaccggc tgactttccc atttgatcga ccatcgactc
     4321 cacgggtacg acatcgatat tcagccgatc gctgagacgg cgaatcgctt ttctggcccc
     4381 ggatgtcagc acccgaaaac caaccacaaa cactaccgcc agcgtggcga gcattatcca
     4441 gatcatgagt tctcctgcgt aatcagaact catgatatct ggaaaccctc gccagttact
     4501 aatccaccag aataatcttc aatgcgaaca gagctgcgac caccaccaca cacaggttca
     4561 gatcgcgcca gcgcccggtg cacactttca tgatgcagta cgacataaag ccgagtgcaa
     4621 tcccttcggt gatcgagaaa gtaaagggca tcatcaccgt ggtaataaac gcaggcaccg
     4681 attcggtaaa atcatcccag ttaacgcgcg ccaggctcga agtcatcagc acgccaacaa
     4741 agattaacgc tccagcggtt gcgtaaggag gaaccatcgc caccagcggt gagaagaaca
     4801 taactaacag gaacataacg ccgaccacga cagccgttag ccccgtgcga ccaccgactg
     4861 ccacaccaga agtactttca atataggcag taacagacga ggtaccgata aatgcgcctg
     4921 ccaccgaact gacgctatca acatacagcg ccttattcat attggggaat ttaccgttgc
     4981 tatctattaa gcccgcttta tcagttacac caattaatgt tcctgatgaa tcaaataggt
     5041 tgatcagcat aaaggagaaa atgataccgg cgagttcaag tgttaacgcg ccgctcaaat
     5101 ctacttcacc aatgacgccg ctaatatcag gcggaatgga atagacgccg ctaaaatgaa
     5161 catcaccgaa aaataatcca cagcaagacg tcaccacaat agaaaccagc accgcggcat
     5221 gaaaatgacg tgatgacaac acggttataa taaaaaaccc taaaatacct aacaacacgc
     5281 cgtgagaact taaatcgcca atcatcacca gcgtgtcttt attggcgaca ataacaccag
     5341 tgttttttaa tcccattaag gcaataaata atccgattcc actggtgata ccaatacgta
     5401 aacttaatgg aatgttggag atcatccagt accggatacg aaacagcgtg agcaaaaata
     5461 gcccaattgc gccccagaat atcgcgccca tcccggtctg ccaggagatc cccatcgccc
     5521 ccacgaccac gaacgcaaag aaggcgttca gccccattgc cggagccagc accacgggta
     5581 agttagcaaa tatccccatc gcaatactgc cgataccggc aatcaaacag gtggtaacaa
     5641 acaccacttt cgggtccatt tgtgccgcgc cgaggatttg cgggttcaca aaaacaatgt
     5701 acaccatggt taaaaaagtg gtcatccccg caatcaattc tgtgcggacg gtggtcccat
     5761 gctgaggtag tttaaataat cgcgaaagcg tccctgattc attactcacg taatcggtat
     5821 tgtcattatt catttttttg tccattggag gagatttaat cccttgcttt tgtaaaacaa
     5881 atgacaacaa cggttcagtg ataattatca catttcaatt gcacattaat agatattctt
     5941 taataatctc gcgacgtttc tttatgataa ataataatca aattgataaa atcaaaatga
     6001 gaaaaatatg aataattcta ttaaccataa atttcatcac attagccggg ctgaatacca
     6061 ggaattgtta gccgtttccc gtggcgacgc tgttgccgat tatattattg ataatgtctc
     6121 tattctcgac ctgatcaatg ccggagaaat ttccggccca attgtgatta aaggacgtta
     6181 cattgccggt gttggcgcag aatacgctga tactccggct ttgcagcgga ttgatgctcg
     6241 cggcgcaacg gcggtgccag ggtttattga tgctcacctg catattgaat ccagcatgat
     6301 gacgccggtc acttttgaaa ccgctaccct gccgcgcggc ctgacgaccg ttatttgcga
     6361 ccctcatgaa atcgtcaacg tgatgggaga agccggattc gcctggtttg cccgctgtgc
     6421 cgaacaggca aggcaaaacc agtacttaca ggtcagctct tgcgtgcccg ctctggaagg
     6481 ctgcgatgtt aacggtgcca gttttaccct tgaacagatg ctcgcctggc gggaccatcc
     6541 gcaggttacc ggccttgcag aaatgatgga ctaccctgcc gtaattaacg ggcagaatgc
     6601 gctgctcgat aaactcgatg catttcgcta cctgacgctg gacggtcact gtccgggttt
     6661 gggtggcaaa gaacttaacg cctatattgc tgcgggtatt gaaaactgcc acgaaagtta
     6721 tcagctggaa gaaggacgcc ggaaattaca actcggcatg tcgttgatga tccgcgaagg
     6781 gtccgctgcc cgcaatctca acgcactggc accgttgatc aacgaattta acagcccgca
     6841 atgcatgctc tgtaccgatg accgtaaccc gtgggagatc gcccatgaag ggcatatcga
     6901 tgccttaatt cgccgcctga tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt
     6961 cgccagctgg tcgacggcgc gccactttgg tctgaatcac ctcggcttac tggcacccgg
     7021 caagcaggcc gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact
     7081 ggtgaaaggc gaaccgattg atgcgcaaac cttacaggcg gaagagtcgg cgagactggc
     7141 gctgtctgcc ccgccatatg gcaataccat tgcccgccag ccagtttccg ccagcgactt
     7201 tgccctgcaa ttcacgcccg gaaaacgcta tcgggtcatt gacgtcatcc ataacgaatt
     7261 gattacgcac tcccgctcca gcgtctacag cgaaaatggt tttgatcgcg atgatgtgtg
     7321 ctttattgcc gtacttgagc gttacgggca acggctggct ccggcctgtg gtttgctcgg
     7381 cggctttggc ctgaatgaag gcgcgctggc tgcgacagtc agccatgaca gccataatat
     7441 tgtggtgatc ggtcgcagtg ccgaagagat ggcgctggcg gtcaatcagg tgattcagga
     7501 tggcggcggg ctgtgcgtgg tacgtaacgg ccaagtacaa agtcatctgc cgttacccat
     7561 tgccgggctg atgagcaccg acacggcgca gtcgctggcg gaacaaattg acgccttgaa
     7621 agccgccgcc cgtgaatgcg gtccgttacc cgatgagccg tttattcaga tggcgtttct
     7681 ttctctgcca gtgatccccg cgctaaaact aaccagtcag gggctatttg atggcgagaa
     7741 gtttgccttc actacgctgg aagtcacgga ataataaaaa aagcccggcg tcatgccggg
     7801 caaaagtcac cagttacgtt tatgccactg tcaactgctg aatttttttc tcgcggcgga
     7861 ttttgcgttc ttccattacc gccactatcg ccatcaggca gatacaacca atcgcggcga
     7921 tatccagcgc ggcgaaggtg cctgcccagc cggtaaggcc gaataccggc gtcccatcgg
     7981 caatcattcc cagacctaac ttggcaaagc tgtcaccaat caggtaagca aaggtgcctt
     8041 taataccatc ggcagcgcca atcgcttttt taggtacaaa gccaacagca gccacaccaa
     8101 tcaacaattg cgggccaaag accaggaaac ccaacgcaaa gagagaagcc agatagatat
     8161 attcgttact ggcatgttga tacacaccga gcgtggcgat aatcagcgcc agcgcgatgc
     8221 aggccaccag gccacggcga ccgttcgcca ggtcagagag ccagccccac agcagcgtac
     8281 cgaccagcgc accagcttca aacagcgtaa agccctgaat cgccaccgct ttagagagtt
     8341 tcagttcctg gaacgcgtat acggttgacc actggtcgat accaatacgt accacataga
     8401 ggaaaatgtt ggcgaagcac agcagccaga tcactttgtt tttcagcaca tactcaacaa
     8461 agatctgcca cttggtcata tcggtagatt ctgtctcttt gtcctcttcg ctgacctcct
     8521 cgccgaacag ttcttcagct ttgccgaggc cataagattc cggggagtcg ctaccgtaac
     8581 gcaggccgat aaaaccgaca atcagcgcga taatcgacgg gaagataaac atgccgatga
     8641 catggccatc gaacaggtaa tttgccccga acagcgccac acctgctgcg cctgcaccgc
     8701 caaggttgtg agaaatattc cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc
     8761 atttggtgat ggtggagtaa ctgcacgaac cgccggtact ctggaaaaag ccgcttaagg
     8821 cgtagaaggc aatcatcagg aacaggctaa ccgagccgct gcccatactg gcactgaagc
     8881 ccagcataca aatggcagag aggataagca tgaacggcag gaattgtttg gtgtttttgc
     8941 cgtcggcgta gtatgaaacc aacgttttac ccacgccata agtgatggag aaacccaggc
     9001 cgatcatccc cagctgcgtc atgctcaacc cgtaggtcga aatcatatcg ttctgcgcga
     9061 tgttaaagtt cttgcgaatc aggtacatcg tcaggtagcc gataaagacc accaggtagg
     9121 attgcatgaa cggtttgaac cacattttgc gccgcacttc gagcggaagg tccagggtcg
     9181 gcttgcgaac ctggtttagg aaagccagca tgggttactc ctgaaatgaa tacctgcgtg
     9241 aggcatgcat tgtaaaaatc agcagcaaat caggcctgag atagcgtcca agaaaaacct
     9301 ggaaaatttc ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc acgcttcacg
     9361 cggtgtctgg gcgttcaaaa agggcagtaa cagcagtgcg gaaatcccgg cggcgatagc
     9421 gataaccaca aaaaatccgc tccagtgcca ggtatcgagc actttcgcca gcggccaacc
     9481 agcaagcgac gcccccagat aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc
     9541 ctctttgtgg gaacactctg ccgccgccat accaattaac atctgcgggc caaagacaaa
     9601 aaaaccaatg gtgaagaagc aggttgcctg catcacgtag ctggcaaatg gcatcagcca
     9661 cagcgagccg actgaaagca aaattccggc ggcgaaaatc aaattcatcg gcccacggtt
     9721 gccgttaaac aatttgtccg agccccaacc ggctaccagc gcaccgataa atccgcccag
     9781 ttcaaacatc gtcactgccg tattcgccgt gaccagatcg acgcccagcg tctcggacat
     9841 atacaaattg ccccagtcgt tgatcgccgc ccggaccaca tagaccagca catagcaaaa
     9901 cgaaagcagc cagatatacg gattcagcaa cacatatttg gtgaggatct ctttacgcgt
     9961 caaccctgcc ccttcttgtt gttgagcaat ttccagcgcg tcgtgccgcc attcaccgac
    10021 cgccggtaaa cctaacgcct gcgggcgatc gcgtagccgc cagcagagaa aaatccccac
    10081 gactatcgcc atacaaccag caatcatcat cccggcacgc cagccgtaat gcagcgcgct
    10141 cgctgccatc acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc
    10201 ccaccaaccg ccgcgctcgg tacgtgaata ccaggccgtt aacagacgcg cacacaccgg
    10261 tgaaccccag ccctggaaaa aggcgttcag cacccagagc acggcaaacg cccatagcga
    10321 cgtcgagaag ccaaacagaa tgttcatgat gcccgtggcg ataagcccta tccccataaa
    10381 ataacgggca tttgagcgat cgctgacaat gccggagaca aacttcgaca cgccgtaagt
    10441 aatgtaaaac agggtcgcta acaggccgat atcgctacgg ctgagcacgc cgttagcaag
    10501 gatttctggt accgcggcgt taaaactttt ccgcgtgaag taaaacagcg cgtaacccag
    10561 ccagatggtc agcagaatat gccgacgcca gtagcgataa cgggcatcaa tttcatgttt
    10621 atcagtcatt aatggcgcat cggcaggcgc tttcagaaac ggcaacatca tcgaacctta
    10681 gacataacgt tgaggtagag aaacgctgac acgcgtgccg tgcagacagg aaatgtgtaa
    10741 tgtgccgccc agcgccgtta cgcgctcgcg cattccggtg aggccaaaac cttgttgccc
    10801 ggaacccggc ggcaaaccgc tgccatcgtc ttcaatcacc agcatcaacc gttcatcctg
    10861 ctgccagcct tgcagcgtaa ccgcgctggc atcggcatgt ttcacaatgt tgttcagccc
    10921 ttcctggcag acacgaaaca gcgtcacgcg ctggttttcg cttaatgctg attcatcgat
    10981 acgccattcg agatggctga caatgccgcg cccttccagc tccatttccc gcatcagtga
    11041 gcggatggcc tgctccaggg tgagatcatc cagctggcgc ggacgtaacc gtcctaacaa
    11101 acggcgcacc gcgtcgtaaa cgcccagcga cagttgttcg atgagctgcc cgctctgctt
    11161 cacactggcg ttatctgccg ccagccgctg aacaatgccc gcctgagtac gaatagcagt
    11221 gatggtctga ccgatatcgt cgtgcagctc acgcgccacg tcgcggcgca cgctctcttc
    11281 ggtttctaac aaacgttcag ccagatgctg attgcgcgcc agttcctttt gtagcgactg
    11341 gttaagttca cgcaaccgct ggatgcctgc gcccagtaac aaccctgtca gactttgcac
    11401 cagcaacgag agtaataaat ccaccagatg atcgcgccag gtttgactgg cgatcagcgc
    11461 gatggcgttc atcaacgtcg caatcagcgc tccttgccag ccatagtgcc aggccagcgc
    11521 gataatcggc agcgccagac agaatggcgt aaagcgcgac agttcgtccg gcaatcccaa
    11581 ctggagccag agactgataa caaacagcag caagtaccag accagatgcc gcccgcgcca
    11641 gttgatgggc tgagaaacca gtgacgggcc gagcggcagc caggtgttat tggcgagata
    11701 gtgccagaac accagacata tcggggccag cgtcagaccg ccagttaaag tcagcaacag
    11761 cgcattccac gactctttgc cgtgccaaag ccagggcagc gactgcaaca acgccgccgc
    11821 cgttaacgcc gcaccctgta acagcaaggt gcgccagtca cgctgatggc gatagcgcga
    11881 gatcagcgct acgggcagta acgtcagtaa actaccgatc atcaataacg gaaaatgggt
    11941 taaaccgacc gcctgcgtta gccagtaaat cagcagccac tccgcaccca gcaatacggg
    12001 ccagtatccg cgcgggcatt gcagcattag ccccagacgc agaccaaacg gaaataacag
    12061 caccgccata tcagggcgct caaccagatg caggctgata ctccataggc aaaaccatgc
    12121 ggcagagaag ataaaaaagc aggcaataac ggtaattaag cgggagaaca acgtcttcat
    12181 caccagccat caaacatgcg gcgcgccagc tctacgtcgt tactgacgcc cagtttttcc
    12241 atcagattgg cgcgatggac gtgtaccgtt ttcggtgaca agcccagttc ggcggcaatc
    12301 tctttcaccg ccattccttg cgccagtttt tccgccacct ggcgttcacg tttggtcagc
    12361 gggtcctgac gaccggatgc cagtttaatg gcaatatccg gcgtcagata acagccgccc
    12421 gtggcaaccg tatgcaccgc agcaatgagt tcatccgggc tacagcgttt ggaaagaaag
    12481 ccgcgtgccc ccgcgttaag cgcctgctca accagcgcag gactgtcgtg aacggagagc
    12541 ataatcgtcg ccataccttt cggcagctgg cttagcagct ccagaccgga gatatcgggc
    12601 atggagatat cgcaaataca cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg
    12661 cgccccgaac caaactcggc aactacctgc aaatcaggtt ccagccccag cagctgcgca
    12721 aagccggagc ggacgatgag gtgatcgtct ataagggcaa cggtgatcat ggtcttgtcc
    12781 tggcgggtaa aaaaaaacgc gcttacctta acgataagcg cgatgttgtt caagccttga
    12841 gcggttactg aaaaaacacc gcgatcttgt taaacatcgt cggatcggac tgattacgct
    12901 gcactttcac gacatcttcc agcttatcga tttggcttat catctgctcc agacgctggt
    12961 cgtcattgac cagtagccag atatggcttt tgtcgctgtc ctgaatcggc agacaaagaa
    13021 tgccttcaac gttaaaagcg cggcgggcaa aaaggccaca aacgtgggtc attacgcccg
    13081 gatggttgcg aacggtgagc tccagaatta cgttgttatg agttgtgttt tgcatggctt
    13141 attcccccac catttcagta ttcgccgcac ctggcggcac catcgggtaa actttttctt
    13201 cggcatcaat gcgcacatgg atcagcgccg ggccagggcg attgattatt tcctgcaatg
    13261 cagcctgcgg atcggcttcg ttattcaaat cacaggtttc gaggccgaat ccggcggcaa
    13321 tctgcataaa gttgattttg cccggatagg tggcggcaaa aacgccttgc tcgtagaaca
    13381 gactctgttg ctgatgcacc agccccagcg cttcgttgtt catcagaatg attttgacat
    13441 ccagctgatt ttcactggcg gtcgccatct cctgaatatt catcatcagg ctgccgtcgc
    13501 cggagaaaca caacactttg cgatccgggt tcgccagcgc cgcgccaatc gccgcaggca
    13561 ggccaaaacc catcgtgccc agcccaccgg aggtcagcca ctggcgtggg cgattgagcg
    13621 gataagcttg cgcggtccac atctgatgct gacccacatc ggtggtgata atcgcattgt
    13681 catcgacaca ggcggcaacg gcgttgatca ggccgtaatg gcttaatgga tcgcacgctt
    13741 tcgggattgg acacgggaat tcgcgctgca aatccgctac caactggtgc cactctgcac
    13801 gcagttgcgc ttccacctgc gggatcaatt gcgccagcac gtcatcaaca tccgcctgaa
    13861 tcgccacatg cggctgcttg attttaccca gctctgcgcg atcgatatcg acatgaataa
    13921 ttttggcatt cggacagaac tgctcggttt tgccaatcgc ccggtcatca aaacgcgcac
    13981 cgagcacaat caacaaatcc gcctcttgca aaatatagtt ggtactgcgc acgccgtgca
    14041 tccccagcat acccagcgac aacggatgcg cttttggcag catgcccagc gccattaaag
    14101 tcatggtggt aggcagttgc gctttctccg ccagttcacg cacccgcgca ggcgcattga
    14161 tcacaccacc acccagataa agcaccgggc gtttggcagc gttaatcatt gtagctgcgt
    14221 cacgaatgct ttcttcgcta aaggagggtg cagcggcttt ttctgccacc gcgggctgag
    14281 cttcaatctc aaaaaccgcc gtttgtacat ccttaggaat gtctatccac accgggctag
    14341 ggcggcctga ttgcgcaatg cggaaggcat cgctcatgac ctgtgggagc tcttcgatat
    14401 gtctgaccag atagttgtgt ttggtgatgg ggatagagat gccgtaggtg tccacttcct
    14461 ggaaggcgtc ggtgccgatc atcgaggcgg gaacctgacc agtgatgcaa atcagcggga
    14521 tggagtccag ccgcgcatca gcaatggcgg tcaccaggtt agtcgcaccc ggcccgctac
    14581 aggccataca gaccgccggt ttaccgtcgg tgcgcgccat tccctgagcg ataaaccccg
    14641 cgccctgttc atggcgagcc agaatatggc ggatttgcgt gctttggctt aaggcgtcgt
    14701 aaacaggcag gatagaaccg cccggaatgc ccgtcacaat cttaatgccc tgctgttcca
    14761 ggaaatgaac gataaattct gcgccggtaa agcgcttacg cgtcgatgtt gtgcccgaac
    14821 ttgccatgct ccagtccttt tcttctgggc cgactttccg ggaggtgctt aaacgaaaaa
    14881 ccccgcccgg tttgcgccgg cggggttttg gaatcgtgtg ttgttccagt ccctacggcg
    14941 cattgccgac gaccaccacc acacgcacga cgaccactgc ggcggatggc gcagttggta
    15001 gtagttttgc gttgaacatg gaagtagtca ttggggacct tgttggtttt gtgtttaaca
    15061 atatttatac aagcacagct ttacagggga gacaatggaa aatttttcag caagggaaaa
    15121 ttgaggggtt gatcacgttt tgtactgaat tgcagataac aaaaaacccc gccggagcga
    15181 ggtttcgtca gccgcctgcg gctggtaacc gcaaagcaca ctgtattatg tcaacactga
    15241 aagtatacgt gttccgcgca gaacgcgcaa tttcggcacg aattttgacg tatttagtgc
    15301 atagttgagt atcgttcaca gtttgcgttt tgtccaaata ttactgttta tttatacagt
    15361 aaacttctat aatatcactg ttcgcaatgt gttatgcggg ggccgcatcg ttacccggcg
    15421 cactaagtcc tggctgaaac gggtggtgcc gtcagcgcct taaccccgcg tgagcacact
    15481 gtgttatgtc aacaagcaca acgtttctcc ttgagatacc gcgtgcacaa cagctggcaa
    15541 caggcagcgg aaaggtacgt cagctggcag tgctcctgaa ccacaggaga cgcgtatgag
    15601 cctggtggat atcgccattc ttatcctcaa actcattgtt gcagcactgc aactgcttga
    15661 tgctgttctg aaatacctga agtaattcag attcaagtcg caccaaaggg gagcgggtaa
    15721 ccgctcccct tttatattta gcgtgcgggt tggagtcgga tgcgatgctg acgcatctta
    15781 tccgccctac catccctccc ggcaacattt attgccgctt ttgtttacat attctgccgc
    15841 taaacaattc cccattcctg gcgtatatct ggctaacatt catcaatgtg atagattcct
    15901 ctcccgcatt tatgggaatg cgtagtgact tattctaatt atttttataa aagcatccgt
    15961 gataatgaaa aggcaaagaa acgtcaattt gttattgatg ttggtattac tcgtggccgt
    16021 cggtcagatg gcgcaaacca tttatattcc agctattgcc gatatggcgc gcgatctcaa
    16081 cgtccgtgaa ggggcggtgc agagcgtaat gggcgcttat ctgctgactt acggtgtctc
    16141 acagctgttt tatggcccga tatccgaccg tgtcggtcgc cgaccagtga tcctcgtcgg
    16201 aatgtccatt tttatgctgg caacgctggt cgcggtcacg acctccagtt tgacggtatt
    16261 gattgccgcc agcgcgatgc aggggatggg caccggcgtt ggcggcgtaa tggcgcgtac
    16321 tttgccgcgt gatttatata aacggacaca gttgcgccat gctaacagcc tgttaaacat
    16381 ggggattctc gtcagtccgt tgctcgcacc gctaatcggc ggtctgctgg atacgatgtg
    16441 gaactggcgc gcctgttatc tctttttgtt ggttctttgt gctggtgtga ccttcagtat
    16501 ggcccgctgg atgccggaaa cgcgtccggt cgatgcaccg cgcacgcgcc tgcttaccag
    16561 ttataaaacg cttttcggta acagcggttt taactgttat ttgctgatgc tgattggcgg
    16621 tctggccggg attgctgcct ttgaagcctg ctccggcgtg ctgatgggcg cggtgttagg
    16681 gctgagcagt atgacggtca gtattttgtt tattctgccg attccggcgg cattttttgg
    16741 ggcatggttt gccgggcgtc ctaataaacg cttctcaacg ttgatgtggc agtcggttat
    16801 ctgctgcctg ctggctggct tgctgatgtg gatccccgac tggtttggcg tgatgaatgt
    16861 ctggacgctg ctcgttcccg ccgcgctgtt ctttttcggt gccgggatgc tgtttccgct
    16921 ggcgaccagc ggcgcgatgg agccgttccc cttcctggcg ggcacggctg gtgcgctggt
    16981 cggcggtctg caaaacattg gttccggcgt gctggcgtcg ctctctgcga tgttgccgca
    17041 aaccggtcag ggcagcctgg ggttgttgat gaccttaatg ggattgttga tcgtgctgtg
    17101 ctggctgccg ctggcgacgc ggatgtcgca tcaggggcag cccgtttaag cgcacgtcac
    17161 cgcagcatcg tcatcagctc catgggagaa cgatgctgct ttatcagatc acgcatcacc
    17221 cgcatatgcg gtgcggagta agaataaaac gcctgatagc ccgcacaaag cacgctgtct
    17281 ggcgtctcct gccgagcgtg cgggcacagg cgatgccagt cgcacgcctg gcagtcacgg
    17341 cgcggttgcg ggttttcgct gccacaccat ttttgtaagg tggcgtcaaa aagctcaatc
    17401 gagatacgac tgatatctgc ccgcacccaa cactcaaaca gggcaattaa aaatttcccc
    17461 cacagctctg ctgtcaccga agcggccgtc agatcgcccc ggtcatcaaa ttcaagatgg
    17521 ggaatgagct gtacggagcg gatgccccgc tgtttgaaat tgtgataaaa atcggcaggt
    17581 tgcagagcat ccttgtactc tacaaccagc ttatgcctgt cctcttcagc atcaatgacc
    17641 tgacttcctg tcatgcgact ctctcaataa atacgataag ttgatgtata tgcgttacag
    17701 caaacagtat tgcgagagat aacaccgcga gggagatcaa aaatttgtca cggactacgt
    17761 gaaattggga aaaatcgcta ttcgtgacat ccattaaatt gcgattacgg gtgtagtgcc
    17821 agaggatcag cgccacgatg gcgaggatgc caatcgaaat ccagaataac atgcccgcct
    17881 ggtgccagtt gtgtttgata gccaacgcca tcaacgcgcc gtagcccagc atggtacgaa
    17941 accacgccag tgatgtgcgc tccggctgca gccctgggtc ggcaatgcgt ctggctttac
    18001 ggctatccgg catacaacac cagtcccata acgatcaccg ccacaaccat caatattaag
    18061 ctgatgatta ataagctgtt ggtgtagggc aggtcctctt tcagacgcat cgctttttca
    18121 ttgcgtaacc agcgcaggta gccatacatt gctaacccgc cggaaaacag acacaacaac
    18181 aacgccagca gttcacgaat gacgggcgtg gcgaaatctg gcgcaagttg atcaagccca
    18241 acccccgccg ccagaaatcc tagtgcggta cggatccacg tcagaaaagt acgctcatta
    18301 gccagcgaga agcggtaatc gggcgcttct ccaaggcggg aaattttcat tgttgctcct
    18361 ttgcaatttc actaacgctc tatctttagc ggtttttttt aagaatttcg tccgcccgtt
    18421 tcctgatatg cttttgccat ccgctacaag gaggtcagat gggtatcata gcgcaaaata
    18481 aaatcagttc attgggcatg ctgttcggtg ccatcgcttt aatgatgggg attatccact
    18541 tttcattcgg ccctttttcc gcaccaccgc caacgcttga aagcatcgtg gcggacaaaa
    18601 cggctgaaat taaaagaggt cttctcgccg gaataaaagg cgaaaaaatt accactgtgg
    18661 agaaaaaaga ggacatggat atcgataaaa tcctcgatca aagcggtatc gcactggcca
    18721 ttgctgcgct actttgcgca tttattggcg gtatgcgtaa ggaaaatcgc tggggcattc
    18781 gcggcgcgct ggtttttggt atcggcatcg tgtgcagcat tttgctgata ttcctgattt
    18841 tttccttcct gaccggcggt tcgctggttt aagcagcctg aataatgccc gccgggtgat
    18901 catcagcggg cattatcgtc agaatttctg ttttttctcc tccaccttca ctccttgcgt
    18961 cggtaggccg gtgtcatagt cgcgtaccac tggcgaatag ccatcttgtg ggcgtgggcg
    19021 aaacgccccc atccagcgcg gtcgtgcatc tttacgccac ggacgcagac tccattggta
    19081 actgcggaac ggatcgcgaa ttttgtccat gtaatccaat aaggcgtcat gcattttgct
    19141 gcgaacgtct gcaaaacgga tatcatcgat caggttatgc atttcatttg ggtcattacg
    19201 gcgatcgtaa agttcatcac tggtgaagag gttgagcacc agtttaaagt catccgtcac
    19261 ccagcaacgc accggaataa aaccgccaaa gctgtcatgc tcaatctcgt agcggttaaa
    19321 ttccaccatc acgccgcgtg gctctttcac ggcaaggata ttttcccccg gcagaatctc
    19381 tggtttttca atatctgcca gcgccatcat tgtcggcagt aaatcaatat gactgactgg
    19441 cgtatcgacc tgtcgccgct cctttactga tcagcttatg tgcgcccatc atttcaccgt
    19501 gatcggaggt ataaatgacc cacgtatttt cacgttgctc tggcgttaag gcattgatga
    19561 cacgtccgat ttggtcatca acaaagtcat tgcaggcaaa atagagcgga tggtgataaa
    19621 gcccgtcatc accgactggc gatggcatcg cctgcgccca taagcggtga tgttccggtt
    19681 tgttcgccag gtcatcctgt gatttctcgc caagttcgta gtaaaaatca gcgtatttct
    19741 ctaaatactc caccggacag gtgaacgggt gattcggctc atcatacgaa accaccatca
    19801 ggaagggttc ctcggcgcgc acgggctgtt gcagaaaatc taccgctcga ttgctgatgc
    19861 ggtgcgccca ggtgaaggtt tcgtcaatat ggttcgcctg taaatcttcg acgctgttta
    19921 ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaagatag ttcgccccat
    19981 caaaccagta atcagcatcc cactccggcg gacactcgcc agtgccgaaa tagtcatgac
    20041 cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta aagtagcgcc
    20101 ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg gactggttag
    20161 cgtagatacc ggtgaatagt ccggcgcgtg caggcgtaca aaccggtgaa caagtgtagg
    20221 cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta ttcagcggtt
    20281 taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcaattgg ggcgtttcat
    20341 aggctatcct tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg ccgccagctg
    20401 tcaaaaatca ggtagacaat cactacggca gcgatgaaat aactgatcat cgctaaccag
    20461 cgcgtaccgt agccgccgaa ttcagccagc ccgacataga cgccaatcat cgcgaacagg
    20521 ataccaattg acgcgatctt gacgtttttc cacggtttca tgtccaccgc aaacgcatct
    20581 ttgaaggtga acggcgtggc gcgcggtttg ataaaaccga tcaccagcat cacgaccacg
    20641 ttgatgcaga acgtacaggc cagcacatag aggaaatgga agtcgaactt caccagatag
    20701 ttgatggtga tgtagctgat tatgccaatc cccatcgcca cttttgccgc cagcgccggg
    20761 atgcgcggga agaaaaagcc cataatgatg atggtaacca gcggcacgtt gtaaatgccg
    20821 ttgagctgtt tcatccagct atacagcccc tgcggcgcgt tggcgatcca cggcgctacc
    20881 aaaaccgaaa cgatagcgat aaagaaacca aatttgcgcc cgacggtgac cagctgctgc
    20941 ggctcggcat tctggttaat gatgcgacgg taaataccca tactgaataa tgtactggcg
    21001 ctattcagaa agccgttgaa ggtactgatc accgcaccaa ataacaccgc gccgaagaaa
    21061 cccaccagtg gcactggcag aacgttattg accagcgtcg ggtaggccat gtcggctttc
    21121 ggtagatcct gatacagatg aaacgcaatc aaccctggca gcaccagtac cagcgggtcg
    21181 agcattttca gcaccgccgt taacagcgcc cccttttgcc cttccgccag gctttttgac
    21241 gccagcgtgc gttgcacgat gccctgattt gtacaccagt aaaaggtgtt caccagaatt
    21301 aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac tgagtttaat
    21361 ttctcggcgt gaacggtggt aatttgctca atgccctgca taaagctgcc cttgcccatc
    21421 gcgatcaggc caaataccgg caccatcaac ccgccgataa ccagcccaat accgttgatg
    21481 gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg catacaaaat tcccgccaga
    21541 ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag aaatctgtag cgattccccg
    21601 acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg cagaaagcag
    21661 acgccggtgg caatgaggaa gcagaagtcg atgataatac gcgtcgtttt atcataacgt
    21721 tcctccagaa aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt
    21781 agcgcgagga agatcagcgt caccgctgaa gtcacttccc agcccatcac cgacatgccg
    21841 cttttgtagg cctgcccgga aagaccaacc agttgttccg tggaaaggtt ggttaacatt
    21901 aacgaagcgg caataaccgg cgcttttagt gaacgtccgg caagaaaata gccttgttgc
    21961 gatccggtat ctgttttgcg aaccttccac taggtgatca ccgccaccag cagcgtaaaa
    22021 ccgacaaaac tcaagatttg taacgaattc atctcatagc ccttatttat gtttatgtcc
    22081 cggacgcatg aatataggga gaggtaaaat gtgtcgggtc aggcagtcag attcttaaaa
    22141 tacaaacgtc gtatccctga acggattgtt ttctgttaag ttcaggttgt gagcataatt
    22201 cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa aaacgaaact
    22261 ccgtacaata ttcccttatt gattaacgaa aatgtgatct ccagcggaat ttctctgatc
    22321 tcgctctggc atacctacgc cgacgagcat taccgggtga tctggccgcg ggacaagaag
    22381 aaaccgctta tcgccaactc atgggttgcg gtgtataccg tacaaggatg tgggaaaatt
    22441 cttttaaaga atggcgaaca aataactctg catggcaact gtattatatt tttaaagcca
    22501 atggatattc actcttatca ctgtgaaggt ttagtcttgg aacagtactg gatggaattt
    22561 acccccacca gtatgatgga tattcccgtt ggtcagcaaa gcgttattta taatggcgaa
    22621 atttataatc aggaactcac cgaagttgct gagttaataa cttcaccaga agcaataaaa
    22681 aataatctgg cagtcgcttt tctgacgaaa attatttatc agtggatttg tcttatgtct
    22741 gcagatggta aaaaagatcc acaacggcgg caaattgaaa aattaattgc cactttacat
    22801 gccagtctgc aacaacgctg gagcgtagct gatatggctg ccacgatccc ctgtagcgaa
    22861 gcctggttgc gtcgtctgtt tttacgctat accggcaaga cgccgaaaga atattacctc
    22921 gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac aaggaaactc ggttggcgaa
    22981 gtcgctgata cgctcaactt cttcgactcc tttcatttca gcaaagcctt taaacataaa
    23041 tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac ggatgccagt
    23101 ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa ctcatccaga
    23161 atagctttgg cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc ctgccacagg
    23221 tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc cacttgctga
    23281 ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc aggccgtagt
    23341 agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc cggctttcaa
    23401 taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg
    23461 cttctgcgac aattgctgcc gggttggagt agttgagcat ctaggcgttt ggtgaatatt
    23521 tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg tacgctattc
    23581 cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg ccgtgggatt ttttcatcca
    23641 gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat aacaaaatca acgtcgctga
    23701 acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctttt tctttaagga
    23761 tgactttaca ggcttcggca atcacttcct gccgcgcgcc atcgttatca taaaatttca
    23821 gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg cctggcgtaa
    23881 aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg cctccgtaat
    23941 ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt cacgcaattg
    24001 ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc
    24061 tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca gtgcaatacg
    24121 tagacgcgtc gcgcaattgt taatgctgga gatattgccg accccgccca gggcttgcag
    24181 gataccggca gcctgatcca gctctttttt tggctctgca gcggtggttt agcctcgcga
    24241 ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc ccggcgtgca
    24301 catgttgaac tgcagaatca gggggcggaa aaccacgaag tacagcaggg taaagcacaa
    24361 cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt
    24421 ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac caaagagata
    24481 cattacggtc gacattgagg ccgccagcac ggcgtgtacc gcaaacagca acggtgaaat
    24541 gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg ttaaggttgc
    24601 gggaatcagc aagcccgcca cttttacccg attttccggt gcggcagtga agtacatcgc
    24661 taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa aaccgccttc
    24721 cgggaacaac gatttcagcg gctcggcgct cagactgaac tcttgcagat gctgcgccca
    24781 gtacatctga atgccgcctt caacagctgc cggaccaaag ataaacggtc cgtagatgaa
    24841 gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc acaccccaag
    24901 cgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta cttttggcca
    24961 gccgagcaat gtcagccagg cacaggggat catcaccagg aaggcgataa tcaccacata
    25021 agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca gacggttatg
    25081 cagcgccgtc acaatgccgg aaatgataat tgcgccgata atgctggtat cgagggtttt
    25141 aatcccggcc atcattgtca gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc
    25201 gaagtagctt ccccaggtca ttcccatcgc attgatgaaa tagttccagg tcaggaaact
    25261 caccatcacc gccagacaag cacgtccctg cgcttgctta gcaaggccaa tgggtaaacc
    25321 gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt caataatgtg
    25381 tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa acatcgggtt
    25441 ttgcagcaag atggcaagac ccaccacaat cccggcgaag ggaaacagca gcactggcgt
    25501 gaacatcgcg ccgccaaagc gttgaatttg actgagcatt tccacatcct cttttctcaa
    25561 ttctgtaagt aggaagcctg attggttatg cggcagacct ggagcgagga taggaagatg
    25621 cgatcggcag aacatgacga cccaatggga ttcgtgatcg ctttcatgct tttcgttggg
    25681 cggtagagat ctacgttttc gcgaaaaagt agacatctgt gagcacaggt acacagcatc
    25741 gaacacatct ttaaaaaaaa gatttttttc aatcgattaa gcagaacttg tgggcgcatt
    25801 acccgtgctt gcaggcaaaa aagagagcta gagatgatct acaaaagcat tgcggagcgg
    25861 ttaagaattc gacttaattc cgcggatttc acgctaaacg gccttcttcc cggtgaaaaa
    25921 aagctggcgg aagagtttgc ggtatcgcgg atgactatcc gtaaagccat tgacctgctg
    25981 gtagcgtggg ggctggtggt ccgccgccac ggcagcggca cttacctggt gcgcaaagat
    26041 gtgctgcatc aaaccgccag cctgaccgga ctggtggagg tgttaaaacg gcagggaaaa
    26101 acggtcacca gccaggtgct gatttttgaa atcatgcctg cgcctccggc cattgccagc
    26161 cagttacgga ttcaaatcaa cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa
    26221 gggaaaccgc taatgctgga agacagctat atgccggtaa aactgttccg taacctttca
    26281 ctgcaacatc tggaagggtc gaagtttgaa tatattgaac aagagtgcgg gattttgatt
    26341 ggcggtaatt atgaaagcct gatgccggtg ctcgccgata gactgctggc gcggcaaatg
    26401 aaggtagcgg aacacacgcc actgctgcgg atcacctcgt tgtcatatag cgagagcggg
    26461 gagtttttga attattcagt gatgttcaga aatgccagcg aatactaggt ggagtaccat
    26521 ttacggcgac tccacccgga aaagagttaa ccgatactcc agaagagcac cgccagtaat
    26581 tggggggtga taatgcgcag gaacatcact aacggataga cagtggcgta agagagcgcc
    26641 gccgcaccac tggttggatg aagattatta gcaaacgcca gcgccggagg atcggtcatg
    26701 gaacctgcca gcatcccgca catggtcagg taattcattt tggctaacat ccgcgccaga
    26761 atgccaacag taatcagcgg aacggcggtg atcagggcac cataaccaat ccagcttagc
    26821 ccttcgccat tgaccagggt attcacaaaa tccccaccag atttcagacc aacgaccgag
    26881 aggaacagca cgatccccag ctcccgcagc gcgaggttgg cacttggcgg cataaaccag
    26941 tacagcttgc cgatactgcc gatacgcccg aggatcaacg ccataatcag cggaccgccc
    27001 gccagcccca gtttcaacgc ggccgggaat cctggcacaa agacgggaat agaacctaac
    27061 aatacgccaa gcccgatgcc aataaacact ggcagcatct gaacctgttg cagtttttgc
    27121 tgcgcattcc ccagcacatt ggcaacggca tcaattgcgg acggacgccc caccaggttg
    27181 aggatatcgc cgaactgcag gctgatatcg ccactggcga ccagttcgac cccggcacgg
    27241 ttcaggcgcg agataacaac gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt
    27301 ccgagcacgt tttcattggt gaccaccaca cgctcgacgc gcaaatcagt gcctttcgta
    27361 gacagcgagg tatcgacctc ctgaccaatc accagttgcg cattatgtaa atccgctggc
    27421 tgccccacca gatgcagcaa atcgcccagt tggataatgg tatctggcga aggtactttt
    27481 agggtttctt ctcgtttcag acgcgagcag ataattttgt cgccgttgag aatcggtaca
    27541 tctttaatgg ctaaatcatg caggttaggg ttctcaacgc gaatattgat agtcttgatc
    27601 agcgcgccgc cattggtgcg tgaagactcg tgctgctgag cttctgtctc gacattgacg
    27661 cggaaaataa cccgcaacat ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac
    27721 gccatcgcgt aactcatccc catctgatcg accatttcca ttggtgtacc caggtcgcgc
    27781 aagatctgct gccctgcccc cagcgctggc gtattggtta ccgcaccgga gaaaatcccc
    27841 agcactaccg gcagtggaat atcaaacagt ttatgcagga tggcggtaac cagaccaccg
    27901 atgatgacga tcagaacagc aaacaggttg aggcgtaatc cggagacgcg caatgaggca
    27961 aagaagcccg gccctacctg aatcccgatg gtataaacga acaggatcag gccaaattcc
    28021 tgaataacat gcagcatatc gctacttaat gtcatccccg cctgagaaac aaaatggccg
    28081 acgatgatcc caccaaacag cacgccgcca atacctaatc ctatgccgcg aaatttgacg
    28141 ttgccgataa acaaaccgac gactgccacc aaagccagaa tactgaccgt taatgctata
    28201 tcactcatcg tccatttctc ttgagataac attttaattg ctacggatta tggctgaatc
    28261 ggtgaagcag atttgcctta tagcgcacat tatcacggga aagaacgtgc cgaaatatct
    28321 taaacagtcg aaatttaacg cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat
    28381 ggcggggcaa agagaatagc tagttagcta tttaacgcgg gacgttcgct gatagcgata
    28441 cgctgcgctg cgatgggttc tggctcatta cgaattaaat caatatgcag taaaccgttt
    28501 acgaaggttg cgccagagac ttccatattt tcagccagcg taaaactcag gctaaatggc
    28561 tgattcataa gcccttgatg cagccatttt ttctcttctt ttggctgctc cggcgtgcct
    28621 tttacgctca ggcgcgtacc ttccagttga atctctaaat cttcctgacg gaaacctgcc
    28681 agcgcaaggg taatgcggta gtggttatcg tcgcttttct caatgttgta cggcgggaag
    28741 ctctggcttt caccggcgtt ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga
    28801 cgcatcagtg gggataaatc gaagttacgc atagtcattt ctccttctaa gaagcgagta
    28861 agtacctgca aatccgaaga ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg
    28921 ccgcgccagg cggccttagg gaattagttg atttcgatac ggcgcggttt tttcgcttcc
    28981 ggaatcacgc gttcgagatc gatatacagc aaaccattta ccaggttagc cccacgaaca
    29041 tgaatgttct cagctaactg gaatttgcgt tcaaagttgc gttcagcgat gccctggtac
    29101 agataggtgc gctctttttg ttcgtcggcg tgagcacctt tcaccaccag cagattatcc
    29161 tgggcggtaa tttccagttc gctctcagca aaaccagcca cagcgatagc aatgcggtaa
    29221 tggttttcgt ctaccagttc aacgttatac ggagggtagc cgccattact ctggctctgg
    29281 ttgttttcta agtggttaaa caaacggtca aatccaatag cagaacggta aagcggggat
    29341 aaatcaaagt tacgcataat caatagctcc tgaaatcagc gagaatgtaa gaccttccac
    29401 aatggacagg tcaggtagcc agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa
    29461 aatgggtctg gaaatgaact tttcaagcct tatcagcgga cttttttttg cagtttatgg
    29521 tctattgcat agactgaggg ggcagcacga aacgttaaag tgcgatagcc gccacagtgc
    29581 gacgtaatga tgatgttatt ttcagcaagg gatcgctata actcatcagg ccaacaccca
    29641 cgatcaacag atgaatttat tatgataaga aatgtgttgt tagcgttcat gatatgcagc
    29701 ggaatgacat tactcggagg atgctccagc gtgatgtccc ataccggggg gaaagaagga
    29761 acgtatcccg gcacccgcgc cagcgccacc atgattggcg acgatgaaac caactggggc
    29821 tctaagtcgc tggcgattct cgatatgccg tttaccgctg tgatggacac gcttttactg
    29881 ccgtgggatg tgttccgcaa agacagctcg gtgcgctcgc gcgttgaaaa aagcgaagcc
    29941 aacgcgcaag ccaccaatgc ggttattcca ccggccagaa tgcctgataa ctgatttagc
    30001 gatcggtttc ggtgatccac agctggccac cttccatcca cgccagccat tgaccatccg
    30061 gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt aaatactcaa
    30121 cctcgccgct ttgcgcatgg gcacaaacaa ttcgattatc cagcacaaag cccaaccatt
    30181 ctcctgacgg atgccagtta aatgcagact gaatatccgt tttgttatgg gttaacttgc
    30241 gcggttcgcc gccctgtggc gagataagcc acagttgcac aatgccgtta tcatcacgca
    30301 ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac ccagtggcgc gggacgttga
    30361 ctaaccccgg ataagcccga tgatgggtaa aggttaaacg tcgctgcacg acgccacgcg
    30421 gtggcgcggg tagggttgtt tccgttccac ttaacggcgc atcacctgcc gatttccagc
    30481 tagcttcatc ttgcggtaac tcaacgataa atagctccgg cactttctcg ccctttggcg
    30541 aaagtgtgtc gccaataaac gccagcgcgt gatttcctac ccatccttct tcataagcac
    30601 gattgatttc atcactgcca ggctgcggcg tgggcgtggt tttactcacc agcacacacc
    30661 agtggctacc gctgtattca cgcggatgtt gcttttgtac gttgaccggg ccaaacggtg
    30721 cagcaacccc gacgtttcgc aaatccagcg ccggatcgag ttgatgcatt acatggtcgt
    30781 tataggtaaa gctccccctt tcaccgttcg ggctaaagac atgcacatgg ctgccaccgc
    30841 gcagcgctcc tggcgtatac ggcgcggtaa tatccattgc atcgagattg ctcaccttgc
    30901 cgccttcagc aatgactccg cgtcgatgat ggaagtcgta atgccatgtt tcatcaggat
    30961 tttcagggcc gtgaataaag acatatttct ctgacttcgg atgaacggtc accacgccga
    31021 cgtgtgcgcc ctgtgacgcg cgatagataa cctcgacctc gcctgtatgg atattcacac
    31081 gctcaatggt ctcgccggta aacgacgccc cagaaggacg cacgtcaaat accagccact
    31141 ggctgtcggg cgtccaggta ttggtattgg tgagcaggtg attacgggga gcaaaggtta
    31201 tctgtttcat ggcgcggtcc tgatacaaaa cggttcccgc catcatactt cacaaggact
    31261 tcactttcag acaatagtct tttgccatcg tgatgaatgt gagtgaaaag atggaacatt
    31321 tcgacgtggc gattattggc ctcggcccgg cagggtcggc gttggcacga aagttagccg
    31381 gcaaaatgca ggtgatcgcg ctggataaaa agcaccagtg cggtactgaa ggtttcagca
    31441 aaccttgcag attttcagcg tcaaaactgt cgacgtcgcc gcatcgctca cgcgtaacta
    31501 ccagcgaagc tatatcaata ttaatcgcca tgctttcgac ttgtggatga aatcgctgat
    31561 ccccgccagc gttgaggttt atcacgatag cctgtgccga aaaatctggc gtgaggatga
    31621 taaatggcat gtcatttttc gtgcagacgg ctgggagcaa catattaccg cccgctatct
    31681 ggtcggtgcc gatggcgcaa actcgatggt gcggcgacat ctctacccgg atcatcaaat
    31741 ccgtaaatat gtcgctatcc agcagtggtt cgcagagaaa catccggtgc cgttctactc
    31801 ctgcatcttt gataatgcga taactgactg ttactcatgg agtatcagca aagatggtta
    31861 ttttatcttt ggcggtgcct atccaatgaa agacggtcag acgcgtttca cgacgctgaa
    31921 agagaaaatg agcgcctttc agttccagtt tggcaaagcg gtgaaaagtg agaaatgcac
    31981 ggtgttgttt ccctcacgct ggcaggattt tgtctgtggt aaggacaacg cctttctgat
    32041 tggtgaagcg gcgggattta tcagcgccag ctcgctggag gggattagct atgcgctgga
    32101 tagcgcagag attctgcgtg cggtgttcct gaaacagccg gagaagagca acgccgctta
    32161 ctggcgcgcc acacgcaaac tgcgtttaaa actcttcggc aagatagtaa aaagccgatg
    32221 cctgaccgca ccggctttaa gaaagtggat tatgcgcagt ggtgtggcgc atattccaca
    32281 gttgaaagat tatccaacgc gcttcacatc acccaccagc aggatgtaag agagcgcgcc
    32341 aatcaacgcg acggcggaga tataaaccag tgcaggcgcg aaaccgtaac cctgcgccag
    32401 gtaccccacc accagcggaa cggtgatgcc gcccagacca ccggcgaagt taaacacgcc
    32461 gccggttaaa ccaatcaggc gcatcggtgc cagagaagag accagcgacc aggtaatcga
    32521 agcaaaaccg ttgccgaaga atgccagcgc catcaggcac ataatcatca tcggatcgtt
    32581 ggtgtagtta gcgcccataa tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt
    32641 acgcgcaaag cccagtgaaa agcccttacg taccagcaga tccgcgaccc agccagagag
    32701 caggacgccg acaaacgccg cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc
    32761 cgtgattcct ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa accacagtgc
    32821 agaagccacc gcaaattgcc caagataaac gccgatcagt ttacgatgga acaccagttt
    32881 ccagtctttg gctgttaacg gctgacgcgc ctctttcttc accggcgcat caccatccac
    32941 cagaccgccg ccatcacgaa tgtaatccag ttcagctttg ctgatacctt tggtcaggcg
    33001 cggcggctga taaaccttaa accaaatcag cgaccagata atgccgatac caccagtgac
    33061 aatgaacacc cagtgccagc tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa
    33121 cgccagaccg acaaactgac cagacgtata aaaaccaacg gcggaagcgc gttcatgttc
    33181 cggaaaccag ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc
    33241 ggttatcgca cgcagaccaa ttaatgacat taatcccgtg gcaaagccct ggaataaagt
    33301 cgccactgac caaccaaata tcgcaataaa ataagtcacg cgagaaccta cgcgatctaa
    33361 aaaccaaccg ccggggatct ggcatagcgt ataaagccag gcgaaggccg aaaatacata
    33421 gcccatttcc gctttcgtaa tgccgaactc ttcctgaata tgggcggaag ccacggccag
    33481 gttagcgcgg tcaacataac aaatgaccac cgtaataaag atcatcacca gcgtcagata
    33541 acgccgacgc cccggctttg ctgcattaac gggaatatcc atagcgagct ttctccagat
    33601 tttgggcata gcgaagccgc tcaccatgcc ctgtaattta cagagggtta tatttttgta
    33661 ttgctgtttt agtgcccgat gaggggctta cgtggcagga attaccactc tgctacgctg
    33721 ttatcttcat gacgccagag cggattacgc cagtccgggg catttttact gaactcaata
    33781 actttagctt cgtcgatttc cacacctaag cccggtttcg ttaacggttt aaagaagccg
    33841 ccaaccatgc tgaagtcttc tttgtttttc acaaagtcga gtaactccgc gcctttgttg
    33901 taatgaatgc ccatactttg ttactgaagt accgcgttat aggaaacaaa gtcgatatgc
    33961 aggcaagccg ccagtgcaat cggtccgaac ggacagtgcg gcgcaagggt cacatcatag
    34021 gcttctgcca ttccagcaat tttgtagcat tcggtaatac cgcctgcatg ggagagatcc
    34081 ggttgcagaa tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa acgtgagaac
    34141 atgcgctcac ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg gtagtattcg
    34201 gcttgttccg ccagcaccgg ctcttcaata aacagcgggc gatacggctc cagttcttta
    34261 atcagcactt tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc aaactcaatc
    34321 tgattgccaa aagcttcacg aatttgtgcc acggtgttga ctgccgcatc taccgcgcgg
    34381 gagttatcaa ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc gaagccgatt
    34441 tcgcgcaggg ttttaatgcc gtcgataaca tccgccggac gatcgccgcc gacccaactg
    34501 taggctttaa ttttgtcgcg aaccaggccg cccatcagtt gccagactgg cgcattcagc
    34561 actttgcctt tgatatccca taacgcctgg tcgatcccgg cgatggcgct catcaggatt
    34621 ggaccgccac gataaaatcc ggcgcgatac atcacttgcc ataagtcgtt gatgcgcgaa
    34681 ggatcctgac caatcaaata gtcacccagc tcgtgaactg ccgcttccac cgtacgggcg
    34741 cggccttcaa tcacgggctc gccccaaccg accacgcctt catcggtttc aattttcagg
    34801 aacatccagc ggggaggtaa acgatacgtg gtaattttgg tgattttcat tgcactgcct
    34861 ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt gcgttctacg gactgcccgg
    34921 cgcgataaag atcgctgcct aagcccgccc caacacaacc tgcgtctatc cactgcgcca
    34981 ggttttctgg cgtcacgccg ccaacggcaa agactgcgat gtccggtggt aataccactt
    35041 ttaacgcttt gatgtattgc ggaccaaaag ccgatgacgg aaatattttc agcgcctgcg
    35101 cgcccgcttc gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg caaacggtca
    35161 tgccgtagcc caccgcgcgg cggatcactt cactatggat attgggcgta acgatgagct
    35221 gacagcccat cctggcgagc gcatcgacct gttcaggttt cagtaccgta cctgcgccaa
    35281 tcaacgcctt gtcgccatac gcatcaacga tggcgggaat gctctgctcc cattgtgggg
    35341 aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca acatgcgcca
    35401 gcgcctcgtc gggcgtaatg ccgcgcaaaa tggcgatcag cgggagttta gtttgccact
    35461 gcatgagcga tgctccttat accagcctga aatgccgtgt cgcccgccac cgccgccacg
    35521 tcgcaaccca tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt tccggcgaca
    35581 agggtgatgg cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc tgcgccaatc
    35641 aacaaaccag agagaaattc gctgacctgt tcgcggagaa gtgttcccag cacatgcgag
    35701 gcgcgaactt caaaaagctg cggcaatatg gcgggcgcat taaggccgcg ctcaaggcca
    35761 gccgcgaagg catcggcaga gttttcctgc ggcggcaaac ctgcgccaat caatgagtga
    35821 tttaacagta aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt gatttgctgg
    35881 ctatcggcct gcacccattt gcaatgcgta ccgggcataa cataaagaga ggaaggagcc
    35941 agagctcgcg cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg gttatcgtca
    36001 tgagagacgc ataatccggg aataatccag atattgtcgc caacagacgt taattgttcg
    36061 ccaatagacg aaaaacgggc aggaacagat aaatacggag caactttcca gccgacattg
    36121 ctgccgacca ttcctgccat caccactggc gtattttctt cacgccagtc ggtcgtgact
    36181 tctgctaaca ccgcagccgg agattttccg ttcaggcgcg tgacgcctgc ttctgattgc
    36241 ctgctctcca ggcagtggtc gccctgataa agccaggcgc gcagattggt cgatccccag
    36301 tcaattgcga tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc gagctggcaa
    36361 tcatggtaag cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc gcatcgaaca
    36421 gcgccttatg ttcctggagc gtttgcggca tgttggcctc atcgcccatc caggttcgtt
    36481 caaaaaccgc ccgctgcagc gaactgatcg caatgctaag ttgctgtaac accgggttat
    36541 gcaccgactg cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt
    36601 cctgattgtt ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga tcgctggagg
    36661 tcgcgcgctc tgctgcccaa cgggcaatcg ccggttccac cagatttcgc acttcgctca
    36721 tggcactgat aagccgtggg tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt
    36781 cgaggtaatt ccactggtta cgcggtgcca caaacgcccc gcgataacgt ttcatttcaa
    36841 tcagccgctt cgccatcagc gaacggaaca cctcacggat gatgttgcgc gaggttgcaa
    36901 actcttcaca gagctccgcc tcagccggaa gtggcgagcc aggtacgtat ttgccgtgaa
    36961 cgatctgttt acccagcgta atgacaatgc gatcggtttt attgagagtc atggagagtc
    37021 cttgtgctct gtatgttctt ctctacttta ccccgatcga tgcataacgc ggcaactttg
    37081 tagtaccagc gtgatgacgt tcgcgtttgc cgtgcgtgta atgtagtaca aacttatatt
    37141 gttgtgctac aatttagatc aaaaaaagaa caatgtataa aaaaatgaca tgcgtcgggc
    37201 agaaatctga aaagggatat caggcgctaa acaggaggga aagaagagta tgctttcaac
    37261 ggcttagcta ctcgtttaaa agattaatca tgaagttgaa ttttaaggga ttttttaagg
    37321 ctgccggttt attcccactg gcgctgatgc tttcaggctg tatctcgtat gctctggttt
    37381 cccataccgc aaagggtagt tcaggaaagt atcaatcgca gtcagacacc atcactgggc
    37441 tatcgcaggc aaaagatagt aatggaacaa aaggctatgt ttttgtaggg gaatcgctgg
    37501 attaccttat cactgatggt gccgatgaca tcgttaagat gctcaatgat ccagcactta
    37561 accggcacaa tattcaggtt gccgatgacg caagatttgt tttaaatgcg gggaaaaaga
    37621 aatttaccgg cacaatatcg ctttactact actggaataa cgaagaagaa aaggcactgg
    37681 caacgcatta tggttttgcc tgtggtgttc aacactgtac caggtcactg gaaaacctaa
    37741 aaggcacaat ccatgagaaa aataaaaaca tggattactc aaaggtgatg gcgttctacc
    37801 atccatttaa agtgcgattt tatgaatact attcacccag aggcattccg gatggtgttt
    37861 ccgcagcatt actgccagtg actgttacgc tggacatcat tactgcaccg ctgcaatttc
    37921 tggttgtata tgcagtaaac caataatcag taagcgggca aacgcgttta tgctgtttgc
    37981 ccgcccacag attaattcag cacatacttc tcaatagcaa acgccacgcc atcttcaagg
    38041 ttggattttg tgacaaagtt cgccatttct ttcaccgaag gaatagcgtt atccatcgcc
    38101 acaccgacgc ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg
    38161 atttcttccg gtttaatacc taacacgtcg gccagtgatt tcacacccgt acctttgtta
    38221 acgcgtttat cgaggatttc gaggaagtac ggcgcacttt tcagcacggt atatttctct
    38281 ttcacttcct gcggaatacg cgcgatagcc tggtcgagga tggcgggttc atcaatcatc
    38341 atcactttca ggaactgggt attggggtcc attttctccg cttcgcagaa caccagcgga
    38401 attgtggcaa cgaaggattc atgcaccgtg tagtagctga tatcgcggtt agcggtgtac
    38461 agcgtggtgc ggtccagggc gtggaaatga gaaccgactt cgcgagagag tttttccagg
    38521 aaacgatagt cgtcatagct gagagcggtt tgcgccacgg tgctaccatc agcggccttc
    38581 tgtaccagcg cgccgttata agtaatgcag tagtcgcccg gctgttccat atgcagctct
    38641 ttcaggtagt tgtgcacacc tgcatacgga cgacccgtcg ttagcacgac attcacgcca
    38701 cgggcgcgag ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggcagc
    38761 agaagggtgc catccatatc gatagcaatg agtttgatag ccatgagttc ccaaggtaga
    38821 ttagttcctg acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa
    38881 aaggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt ttccctttca
    38941 gcagttccat gcctgcggaa agcagatcgt tattggcttg tgctgacact tcaccttgcg
    39001 gtgagagcgc atcaataatc ttcggcaatt gctctgccag taaactggaa gccgtactgg
    39061 tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgagc gccgattcca
    39121 gttgctcgcc gctaactgat tgatttctct gttgattact caaccaagtt gagagaatgg
    39181 cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccaccc tgctcctcaa
    39241 cccaacttaa aatcgcctga tatttccccg catcgccttt cagaaaggca ccgacaactt
    39301 catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca aaaagtatag
    39361 atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga tctccgccct
    39421 tttcttatag cttcttgccg gatgcggcgt gaacgcctta tccggcctac aaaatcatgc
    39481 aaaatcaata tattgcagga gtctcgtagg actaataagc gtagcgcatc aggcacgctc
    39541 gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct cttcaataaa
    39601 cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaactggt cagcggcaat
    39661 cgcatcctta acggtaacgc gcagcatacg acggctttcc gggtccatgg tggtttccca
    39721 cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga ggccacgacg
    39781 ggactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct gacgacgctc
    39841 gccacgttcg ataaacgcat cttcttccag caagccacgc agtttctcac ccagcgtgca
    39901 gatacgacga tattcgccac cggtgataaa ctcgtgatcc agcggatagt cagtatccac
    39961 accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat tggtgtggac
    40021 atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt
    40081 cacccagcgg gtaacggtct gctcatcaga aaggtcagct tccgtcaacg tcggctgata
    40141 gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga tcattttctg
    40201 cgtcgcgttg tactcagata ccagtttctc taacgcttcg ccagccaatg ccggtgcact
    40261 ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact gatccatcgc
    40321 ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca gcggcggctg
    40381 agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga agaaggtcaa
    40441 cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg
    40501 ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa gcgcggtgat
    40561 aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct cgacgttaag
    40621 gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga
    40681 gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat cgcgttcctg
    40741 gcagtctgcc agtttgcccg gcaggccagc taagtcgagc gcacctttac ggcgggtcat
    40801 ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt tgccaaccac
    40861 gattttcgca tcagttgggt tttccagcag gtattccgcc agcagttcgt tcatctgctg
    40921 ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg aggagaattt
    40981 cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt
    41041 ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcat tcagggtacg
    41101 ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt
    41161 ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcagcgcca cttcaacgcc
    41221 aataccgtct ttttcggtgg agaagtagaa gatatttggg tggatcggcg ttttgttctt
    41281 gttcagatat tcaacgaacg ctttgatgcc gccttcatag tggaagtggt cttctttgcc
    41341 gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca actcacgcag
    41401 acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt cgaggctggg
    41461 ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca gcggagcctg
    41521 cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgaa taaccagctc
    41581 cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac cgccggacac
    41641 tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga tcacttccgc
    41701 cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac
    41761 agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac ccgcgagcgc
    41821 ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc
    41881 cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt tcaggacttt
    41941 gatactggag gagtcataag aattcgacat caacgtttct cgctcattta tacttgggtt
    42001 aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc gacatgtcta
    42061 taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc
    42121 tggcaagcaa cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct atcaggtaga
    42181 gacaccgccg cccgctttca cgagtgagga actctccttg cgccagacgt aaggcgcaca
    42241 tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa
    42301 tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa
    42361 aattacgttc cagcacctca gcgtactctg tctctttctc ccagccgcgc tggaaagaga
    42421 aagtcagaga aaactcaggg agaaattgct tacaggtatc atccatatcg gccgcgatac
    42481 cggcgctata ctccgcgcgc caggtgctga tttgctccgc cagcgggatc agttctttat
    42541 cccacgggcg tagctgttcg taacgagtca cctggcgcag cgccgcattg cgctgcttga
    42601 gcaatcgctt gagattgctc caggcggtga aaaatctagg ttcgttgtga aagcatcccc
    42661 agtcgaggaa tgctcttctg tatttggggc cgccgttgag taaagtaaac ccttctggcg
    42721 ttatcaactg catcggcatc aggtgcgcca gttccgcgac tttatgcccg tccgtaccgt
    42781 caatacggac tttgctgtcg ccctgtttgt ctttggttaa gccaatcgcc gtctcgcgct
    42841 cttcgccctg taatcgcccg tggagaacaa atgcctcctg ctcatggcga atgacgcgac
    42901 caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct tccagcacgc
    42961 tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat aaagcgagat
    43021 ccgcggtttc aatattgcgg aaatcgcgga tcaacaagcg ggtgagggac attacagtct
    43081 cattggcatg acaacatagg cagccgactg tgatgcggca tcttcaatct gcacgctgga
    43141 aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca gaacatccag
    43201 cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa cgtcgaggat
    43261 ctcttccgct tcttcctgtt ccgggttgtt ggcagtaatt ttcagctggt tttcgctgac
    43321 atacaggcgc acaccgcgga atttctcgtt agagagaatc gccgcgcgag caaacgcctg
    43381 cttgagcaga tcgcagcctg cttccagatg tttgtccggg ttcttcggca gaacgcgacg
    43441 gtaatccggg aagcgaccat ccaccagttt cgaggtgaaa ataaagtcgc caacgtgggc
    43501 acgaatgtta ttgctgccaa tctgcacacg cagcagattg tcgccgccgt cgagcatacg
    43561 catcagttca atcacgcctt tacgcggtac gatcaccgaa tggcttggca aagattgacc
    43621 aattggcatt gaacagaccg ccagacggtg gccgtcggtt gccacggtgc gcagttcttc
    43681 accttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat gcgccatcga
    43741 aaactgggtt gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc
    43801 gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg
    43861 gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa tttccgcgcc
    43921 ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg
    43981 ctcgtgtggc tgaaccagcg caacgcgtgc caccatttcc atctcgagat cagtaccggt
    44041 cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg gtagcgtagg
    44101 acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct cacgttctac
    44161 ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa tcttctttga
    44221 tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc accgtcgtgt
    44281 ggtcacgccc accaaacgca tcgccaatct ctggcagact gtggttagtc agctctttcg
    44341 ctagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga
    44401 gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg tcgatggtga
    44461 ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag tcgatggtga
    44521 tcgcccgtcc ggtaaagttg gcgttagcaa taacgcggtt cagcgcccct tccagctcac
    44581 gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcacccggc aaacgaatgt
    44641 cattttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct ggcggttcga
    44701 tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg ccgttgatct
    44761 ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc agggcgttga
    44821 aggtgtggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga atatcgtcga
    44881 tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca
    44941 gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact ttggcattcg
    45001 gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta cccagacccg
    45061 tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc acctggcgag
    45121 ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac gtgtgtttga
    45181 cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc caacctgagc
    45241 gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt
    45301 tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca
    45361 gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta ttaaggtact
    45421 tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggacagc gtgttatcgc
    45481 tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt aactcatcct
    45541 gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc actcgaacaa
    45601 aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg aaaagaccct
    45661 gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag atcctgcaaa
    45721 acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt tctcacagat
    45781 tttccctatt tatccacagg actttccaga actcgctaag tgtaaacgat cctgccgcga
    45841 ggcgggcacg atttacgccg catattggaa aatttaatga ccatagacaa aaattggctt
    45901 aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc cgtataatcc
    45961 tccacccggc gcgccatgct ggtttccact ggtgtgaggc cgtacatttt ccctgcgaaa
    46021 aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggtgtgt acaattatta
    46081 caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt ttcggcggat
    46141 atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga aacgcacttt
    46201 tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta tggctactaa
    46261 aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc
    46321 taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag ttacgcttgt
    46381 taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc acgccgcaaa
    46441 ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt acagtcgcca
    46501 agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg cgtgaaagct
    46561 tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg
    46621 ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg cgccgccact
    46681 gtctcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc tgattagtcc
    46741 gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa ttgaggcatt
    46801 gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat taaaatgcca
    46861 ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata ccagagaaca
    46921 ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt gtctttcatg
    46981 atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca gaccacgcag
    47041 acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag tggccagggg
    47101 aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg tggtggtgat
    47161 gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca gccgttccag
    47221 ctgttggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac cggtcgtgat
    47281 ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa agacgcttat
    47341 gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga cgcggcaggc
    47401 aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac
    47461 aacgtgcaga acgctggcga gaaaccgctg gaaatctcct cgtttggtca gttgaagcaa
    47521 tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact gcacaccttc
    47581 cgtggcgcgg cgtactccac gcctgacgag aagtatgaga aatacaagtt cgataccatt
    47641 gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcgat gctgcaacag
    47701 tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta taccgctaat
    47761 ctgggtaacg gcatcgtcgc tatcggctat aaatctcagc cggtactggt tcagcctggt
    47821 cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga caaaatggca
    47881 gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt catctctcag
    47941 ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg cttctccatt
    48001 atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc
    48061 tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga gcgtctgggc
    48121 gatgacaaac agcgtatcag tcaggaaatg atggcgctgt acaaagctga gaaggttaac
    48181 ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc gttgtactac
    48241 atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat ccacgacctg
    48301 tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat gttcttcatt
    48361 cagaagatgt cgccgaccac tgtgaccgac ccgatgcagc agaagatcat gacctttatg
    48421 ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtact gtactatatc
    48481 gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct ggaaaaacgt
    48541 ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct aaaataaggg
    48601 cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc atccgccaca
    48661 caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc cacacctccg
    48721 ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga agttgccgaa
    48781 accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt taaagacgcc
    48841 gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc
    48901 ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga cctgctgtta
    48961 aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc
    49021 gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga tcttatcgac
    49081 gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc attctccgca
    49141 cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga agcggcaatt
    49201 gatttccctg atgaagagat cgatttcctc tccgacggca aaattgaagc ccagcttaat
    49261 gacgtgatgg ccgatctcga tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc
    49321 gaagggatga aagtagtgat tgccggacgt cctaatgccg gtaaatcgag cctgttaaac
    49381 gcgctggcgg gccgtgaagc ggcaatcgta accgatatcg ccggaaccac gcgtgacgtg
    49441 ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgacac tgccgggcta
    49501 cgtgaggcca gtgacgaagt ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa
    49561 caggccgacc gcgtgctgtt tatggtcgat ggcaccacga cagacgccgt tgatccggca
    49621 gagatctggc cggaatttat cgcccgtctg cccgcgaaac tgccgatcac cgtggtacgc
    49681 aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta
    49741 attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca tctcaaacag
    49801 agcatgggct ttgacaccaa tatggaaggc ggcttcctgg cgcgccgtcg ccacttacag
    49861 gcgctggaac aggcagcgga gcatttgcag caaggtaaag cgcaactgtt gggagcctgg
    49921 gcaggtgaac tgctggcgga agagttgcgc ctggcacagc agaacttaag cgaaatcacc
    49981 ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg tattggtaag
    50041 taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc attgttagca
    50101 ctcctgttat gcctcaaccc ttttttaaac attaaa
//
LOCUS       DAJTRQ010000016        43200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000016 DAJTRQ010000000
VERSION     DAJTRQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..177
                     /locus_tag="OO332_001037"
     CDS             <1..177
                     /locus_tag="OO332_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5941603.1"
                     /translation="YFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRTI
                     NDYGAETPQLNCKS"
     gene            202..2801
                     /locus_tag="OO332_001038"
                     /pseudo
     CDS             202..2801
                     /locus_tag="OO332_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            2792..3862
                     /locus_tag="OO332_001039"
     CDS             2792..3862
                     /locus_tag="OO332_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5941604.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3874..4416
                     /locus_tag="OO332_001040"
     CDS             3874..4416
                     /locus_tag="OO332_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5941605.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4424..4939
                     /locus_tag="OO332_001041"
     CDS             4424..4939
                     /locus_tag="OO332_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5941606.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4905..5642
                     /locus_tag="OO332_001042"
     CDS             4905..5642
                     /locus_tag="OO332_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5941607.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5753..6763
                     /gene="pyrD"
                     /locus_tag="OO332_001043"
     CDS             5753..6763
                     /gene="pyrD"
                     /locus_tag="OO332_001043"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5941608.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6937..7479
                     /gene="zapC"
                     /locus_tag="OO332_001044"
     CDS             6937..7479
                     /gene="zapC"
                     /locus_tag="OO332_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5941609.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7476..8585)
                     /gene="ycbX"
                     /locus_tag="OO332_001045"
     CDS             complement(7476..8585)
                     /gene="ycbX"
                     /locus_tag="OO332_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5941610.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8829..10937
                     /gene="rlmKL"
                     /locus_tag="OO332_001046"
     CDS             8829..10937
                     /gene="rlmKL"
                     /locus_tag="OO332_001046"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5941611.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10949..12856
                     /locus_tag="OO332_001047"
                     /pseudo
     CDS             10949..12856
                     /locus_tag="OO332_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12986..14239
                     /gene="pqiA"
                     /locus_tag="OO332_001048"
     CDS             12986..14239
                     /gene="pqiA"
                     /locus_tag="OO332_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5941612.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14244..15884
                     /gene="pqiB"
                     /locus_tag="OO332_001049"
     CDS             14244..15884
                     /gene="pqiB"
                     /locus_tag="OO332_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5941613.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15881..16444
                     /gene="pqiC"
                     /locus_tag="OO332_001050"
     CDS             15881..16444
                     /gene="pqiC"
                     /locus_tag="OO332_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5941614.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16700..16867
                     /gene="rmf"
                     /locus_tag="OO332_001051"
     CDS             16700..16867
                     /gene="rmf"
                     /locus_tag="OO332_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5941615.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16937..17455)
                     /gene="fabA"
                     /locus_tag="OO332_001052"
     CDS             complement(16937..17455)
                     /gene="fabA"
                     /locus_tag="OO332_001052"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5941616.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17524..19284)
                     /locus_tag="OO332_001053"
     CDS             complement(17524..19284)
                     /locus_tag="OO332_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5941617.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19469..19921
                     /gene="matP"
                     /locus_tag="OO332_001054"
     CDS             19469..19921
                     /gene="matP"
                     /locus_tag="OO332_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5941618.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19997..21043)
                     /gene="ompA"
                     /locus_tag="OO332_001055"
     CDS             complement(19997..21043)
                     /gene="ompA"
                     /locus_tag="OO332_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5941619.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21399..21908)
                     /gene="sulA"
                     /locus_tag="OO332_001056"
     CDS             complement(21399..21908)
                     /gene="sulA"
                     /locus_tag="OO332_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5941620.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22127..22756
                     /gene="sxy"
                     /locus_tag="OO332_001057"
     CDS             22127..22756
                     /gene="sxy"
                     /locus_tag="OO332_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5941621.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22719..24881)
                     /gene="yccS"
                     /locus_tag="OO332_001058"
     CDS             complement(22719..24881)
                     /gene="yccS"
                     /locus_tag="OO332_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5941622.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(24891..25337)
                     /locus_tag="OO332_001059"
     CDS             complement(24891..25337)
                     /locus_tag="OO332_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5941623.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25460..27514
                     /gene="helD"
                     /locus_tag="OO332_001060"
     CDS             25460..27514
                     /gene="helD"
                     /locus_tag="OO332_001060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5941624.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27546..28004)
                     /gene="mgsA"
                     /locus_tag="OO332_001061"
     CDS             complement(27546..28004)
                     /gene="mgsA"
                     /locus_tag="OO332_001061"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5941625.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28100..28762)
                     /locus_tag="OO332_001062"
     CDS             complement(28100..28762)
                     /locus_tag="OO332_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5941626.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28935..29348
                     /locus_tag="OO332_001063"
     CDS             28935..29348
                     /locus_tag="OO332_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5941627.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29393..29710)
                     /gene="hspQ"
                     /locus_tag="OO332_001064"
     CDS             complement(29393..29710)
                     /gene="hspQ"
                     /locus_tag="OO332_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5941628.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29768..30958)
                     /gene="rlmI"
                     /locus_tag="OO332_001065"
     CDS             complement(29768..30958)
                     /gene="rlmI"
                     /locus_tag="OO332_001065"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5941629.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31053..31331
                     /gene="yccX"
                     /locus_tag="OO332_001066"
     CDS             31053..31331
                     /gene="yccX"
                     /locus_tag="OO332_001066"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5941630.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31328..31657)
                     /gene="tusE"
                     /locus_tag="OO332_001067"
     CDS             complement(31328..31657)
                     /gene="tusE"
                     /locus_tag="OO332_001067"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5941631.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31748..32407)
                     /gene="yccA"
                     /locus_tag="OO332_001068"
     CDS             complement(31748..32407)
                     /gene="yccA"
                     /locus_tag="OO332_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5941632.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32614..32701)
                     /locus_tag="OO332_001069"
     tRNA            complement(32614..32701)
                     /locus_tag="OO332_001069"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32665..32667),aa:Ser,seq:tga)
     gene            33127..34245
                     /gene="hyaA"
                     /locus_tag="OO332_001070"
     CDS             33127..34245
                     /gene="hyaA"
                     /locus_tag="OO332_001070"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5941633.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34242..36000
                     /gene="hyaB"
                     /locus_tag="OO332_001071"
                     /pseudo
     CDS             34242..36000
                     /gene="hyaB"
                     /locus_tag="OO332_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36019..36726
                     /gene="hyaC"
                     /locus_tag="OO332_001072"
     CDS             36019..36726
                     /gene="hyaC"
                     /locus_tag="OO332_001072"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5941634.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36723..37310
                     /locus_tag="OO332_001073"
     CDS             36723..37310
                     /locus_tag="OO332_001073"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5941635.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37307..37705
                     /gene="hyaE"
                     /locus_tag="OO332_001074"
     CDS             37307..37705
                     /gene="hyaE"
                     /locus_tag="OO332_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5941636.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37702..38559
                     /locus_tag="OO332_001075"
     CDS             37702..38559
                     /locus_tag="OO332_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5941637.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38693..40237
                     /gene="appC"
                     /locus_tag="OO332_001076"
     CDS             38693..40237
                     /gene="appC"
                     /locus_tag="OO332_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5941638.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40249..41385
                     /gene="appB"
                     /locus_tag="OO332_001077"
     CDS             40249..41385
                     /gene="appB"
                     /locus_tag="OO332_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5941639.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41397..41489
                     /gene="cbdX"
                     /locus_tag="OO332_001078"
     CDS             41397..41489
                     /gene="cbdX"
                     /locus_tag="OO332_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5941640.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41569..42867
                     /gene="appA"
                     /locus_tag="OO332_001079"
     CDS             41569..42867
                     /gene="appA"
                     /locus_tag="OO332_001079"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5941641.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42982..>43200)
                     /locus_tag="OO332_001080"
     CDS             complement(42982..>43200)
                     /locus_tag="OO332_001080"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Wzc; catalyzes the autophosphorylation on tyrosine
                     residues which downregulates the biosynthesis of colonic
                     acid (an extracellular polysaccharide); Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5941642.1"
                     /translation="SDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIKGA
                     ILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10742 a        10517 c        11503 g        10438 t
ORIGIN      
        1 tatttcatta ccctgacaga cctgaaaata ggtggaaaag tagttaaaaa tcaaatgatt
       61 tccccctttg ataaatacca attctctctg ccaaaggggg ccaaaaatag cagcgtaacg
      121 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
      181 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      241 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gtccaatgca
      301 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      361 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      421 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      481 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      541 tttccggctt ttaaacaact ggacaaacaa gcgtgtgtgc cattggcgga gattattccg
      601 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      661 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      721 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      781 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      841 ttgcgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      901 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      961 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
     1021 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
     1081 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1141 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1201 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1261 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1321 agtaatgaac agcaggagag caaatttgcc caggccacgt tgcagtgggg cggaccgtgg
     1381 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1441 ctgggattta accttggtga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1501 acgctggcag agcaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1561 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1621 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1681 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1741 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1801 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1861 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1921 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1981 agtaatgata gctataccag gaaaaaaatt acgcctggat gacctctaac accagtatcg
     2041 ataacgaagg gcacattaca caaaacctgg gtttaacgga gacactactc gatgacggta
     2101 acctgagcta cagcgtgcaa cagggatata acagcgaggg gaaaacggct aatggtagcg
     2161 ccagcatgga ctacaaaggg gcgtttgcag atgcccgagt gggctacaac tacagcgata
     2221 acggcagtca acaacaactg aactacgctc tttcaggcag tttagttgcc cattcacagg
     2281 gcattaccct ggggcaatcg ctgggggaaa ctaacgtgct gattgtagca ccaggcgcag
     2341 agaatactcg tgtggcgaac agcaccgggc tgaaaactga ctggcgcgga tataccgttg
     2401 tcccctatgc cacttcttat cgggaaaatc gaatcgcact tgatgcggca tcgttaaaac
     2461 gtaacgtgga tcttgaaaat gcagtagtca acgtggttcc caccaaaggg gcgttggttc
     2521 tggcggagtt caatgcccat gcgggagcaa gggtattaat gaaaacatca aagcagggta
     2581 taccgctgcg ttttggcgcg atagcgacgc tggacggcat acagactaat agcggcatta
     2641 ttgatgatga tggctcgctc tatatgtccg gtttgcccgc tcagggagcg ataactgtac
     2701 gctggggcga agctcccgat caaatttgtc atatcagtta ccagcttacc gaacaacaaa
     2761 ttaactctgc gattacgcga atggatgcca tatgcagata atctttggag aaaaatgcgt
     2821 gttattacta cgactatttt ttgccgccgt cttaatgcta tggtgcgctc aaactgctgc
     2881 ttatatcggg cagtgtcata ccactcaggg gaatccgtat attggcgtca attttggcgt
     2941 taaaactctg gatgaagaag aaaatacggc tggagtagtt aaagacaaat tttatcagtg
     3001 gaacgaatcg agtgattatt atgtttcctg tgattgcgat aaagacaatg tcagaagtgg
     3061 ccgatgggca ttcgccgcgg attcaccgtt agtctattta ggcgacaact ggtacaaaat
     3121 taatgactat cttgccgcca aagttttatt gcaggttaaa ggcagttctc ctactgcggt
     3181 tcctttcgaa aacgtgggca caggggcaga tacacgatgg catatttgcg atcccggcgg
     3241 tcaacgttta ggtggccagg gggctagcgg taatagcggt agcttttccc tgaaaatatt
     3301 gcagccgttc gttggttcgg tcgtcattcc tcctatggcg ctggcgcgat tatttgaatg
     3361 ctacaacata cccgcaggtg attcctgcac gactactggt acaccggttt tagtgtatta
     3421 cctgtctggt acgatcaatt cacttggctc atgttccgtc aatgccggag aaacaatcga
     3481 ggttgatctg ggcgacgtat ttgcggctaa ctttcgtgtt gtagggcata agcctcttgg
     3541 ggccagaacg gcagaacttg caattccggt caggtgtaac acgggaaacg cagggttagt
     3601 taacgtcaat ctgagtctga cggcaacctc agaccccagc tatccccagg tgattaagac
     3661 gtcacgtcct ggcgtgggcg tggtggtgac cgatagccag aacaacatta tttcccctgc
     3721 tggtgggaca ctaccgctct ctattcctga tgatgcagac agtatcgcgc gaatgaatgt
     3781 ctatccagtc agcacgacag gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3841 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3901 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3961 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     4021 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     4081 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4141 ggcgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4201 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc aaattcttga tgcgcaaagt
     4261 cagcaagaaa tccagctcaa tcaggtccag cctcttacgc ccttaaaagc cggggataac
     4321 acactcaaat atcaacttcg ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg
     4381 acggcggttc tctattttga tctggtttac cagtgaagga gtgatgttga aacggataat
     4441 ctggattctg ttcttactgg gattaacggg gggctgtgag ctatttgccc atgatggcac
     4501 ggtcaacatt agcggatcgt ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca
     4561 aatcaatgtg cagttaggcg atgtctcgct gacacgtttt tttcatggga attatggccc
     4621 ggagaaatct ttcatcatta atttgcagga ttgcggaaca gatgtcagca cggttgatgt
     4681 caccttttca ggaaccctcg acggagtgca gagcgaaatg ctgtccattg aaagtggtac
     4741 ggatgcagcc agtgggctgg caattgcgat tctggatgat gcgaagatat tgattccgct
     4801 taaccaggcg agtaaagact acagcctaca tagcggtaag gtgccattaa ctttttatgc
     4861 gcaattgcga cctgtcaata gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt
     4921 tgtacttcat tatgactaat acgtggaaca gattagcgct cttgattttc gccgttttat
     4981 cgctgctggt ggcgggtgaa ttgcaggcag gagtggtggt cggcggaacg cgatttatct
     5041 ttccggcaga cagagaatcg atatctattt tactgactaa taccagtcag gaatcttggc
     5101 ttattaacag caaaatcaat cgcccaacgc gttgggccgg gggggaagcg tcgacagtgc
     5161 cagcactatt actggccgct ccgccactta ttctcctgaa gcccggtact acaggcacgt
     5221 tgcgcttgct gagaacggaa agcgacatct tgcctgtgga tcgcgaaacg ctatttgagt
     5281 taagcattgc cagcgtgcca tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatgc
     5341 gctcggtatt taaactgttc tggcgacacg aaggtttgcc gggcgacccg ctggaagctt
     5401 atcaacaatt acgctggaca cggaattcgc agggtgtaca actcactaac ccaacgcctt
     5461 actacattaa cctgattcag gtgagcgtaa atggtaaagc gttaagtaat gcgggagtcg
     5521 tgccgcctaa aagccagcgt cagacaaact ggtgtcaggt aatagcaccg tgtcatgtcg
     5581 cctggcggac gataaatgat tacggcgggt tgagtgcaaa gaaggagcaa aatctgccct
     5641 gaaacaggtt ctgaaaacgt ttgcgttttt tttgccgcag gtcaattccc ttttggtccg
     5701 aactcgcaca taatacgccc ccggtttgca caccgggaat ccaggagagt tcatgtacta
     5761 ccccttcgtt cgtaaagccc ttttccagct cgatccagag cgcgctcatg agtttacttt
     5821 tcagcaatta cgccgcatta ccggaacacc gtttgaagca ctggtgcggc agaaagtgcc
     5881 tgcgaaacct gttaactgca tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg
     5941 tcttgataaa gacggggagt gcattgatgc gttaggcgcg atgggatttg gatcgatcga
     6001 gatcggtacc gtcacgccac gtccacagcc tggtaatgac aagccgcgtc tctttcgtct
     6061 ggtagatgcc gaaggtttga tcaaccgtat gggctttaat aatcttggcg ttgataacct
     6121 cgtagagaac gtaaaaaagg cccattatga cggcgtcttg ggtattaata tcggcaaaaa
     6181 taaagatacg ccagtggagc agggcaaaga tgactatctg atttgtatgg aaaaaatcta
     6241 tgcctatgcg ggatatatcg ccatcaatat ttcatcgccg aataccccag gattacgcac
     6301 gctgcaatat ggtgaagcgc tggatgatct cttaaccgcg attaaaaata agcaaaatga
     6361 tttgcaagcg atgcaccata aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc
     6421 tgaagacgaa ttgatccagg ttgccgatag tttagttcgc cataatattg atggcgttat
     6481 tgcaaccaat accactctcg atcgttctct ggttcaggga atgaaaaatt gcgatcaaac
     6541 cggtggctta agtggtcgtc cgcttcagtt aaaaagcaca gaaattattc gccgcttgtc
     6601 actagaatta aacggtcgct taccgatcat cggtgttggc ggcatcgact cggttatcgc
     6661 tgcgcgtgaa aagattgctg cgggtgcctc actggtgcaa atttattctg gttttatttt
     6721 taaaggtccg ccgctgatta aagaaatcgt tacccatatc taattatttc ttcgaattcc
     6781 tctttataag ataatcaggg ctttattttc agccctggtt gttttatatt catcgctgtt
     6841 gcttatttag acattttgta cttttattgt tgaggttatt aagcgaagca acaatggatt
     6901 gtgttgctgc ggttttatag ggtaggggag aggcagatgc gaattaaacc agacgataac
     6961 tggcgttggt attacgatga agagcacgat cgaatgatgc tcgatttagc caatggtatg
     7021 ctatttcgct cacgttttgc gcgcaaaatg ttgactccag atgccttttc tcccgcaggc
     7081 ttttgcgttg atgatgccgc gctctatttc tcttttgaag aaaagtgccg cgattttaat
     7141 ttatctaaag accaaaaagc cgaactggtg ctgaatgcac tggtagcaat tcgttatctc
     7201 aaaccgcaaa tgccgaaaag ctggcatttt gtctcccatg gtgaaatgtg ggtgcccatg
     7261 ccgggcgatg cggcctgcgt ttggttgagc gatactcacg agcaggtcaa tttgctggtc
     7321 gttgaatctg gcgaaaatgc cgcgctatgc ctgctggcac aaccctgcgt tgttattgcg
     7381 ggtcgcgcga tgcagttggg cgacgccatt aaaatcatga acgacaggct gaaaccgcag
     7441 gtgaatgttg acagcttcag cctcgaacag gcagtctaac gcgtcagctt aagggcagtc
     7501 ttcggtacac agctacagca aagaatggtg ccatcatcgc ccattgctga ttttttcagc
     7561 ggcgtgactt cgccttctaa aagctgaaca cggcaacttc cacaaatgcc cgcgcggcaa
     7621 gaataaggga tacgaattcc ctgattttct aattgctcca gcaacacttg ttggttattt
     7681 ccacgaaatg cctgtccctg ccagtcaata tctacatttg cgtccggttg ttgcgtgatg
     7741 ttggcggtat catcagcggc agctgcgccg taaatttttg ccggagccgt tgccagaatt
     7801 tccacctcat cgccaacgcg aatcacgccg ctattacggg caattaaatt ctgaccaaaa
     7861 tcgacatcgc cgttatcctg ggcagtgcgg aaagattgca atgtttttaa tggttcgcct
     7921 gccggatgtt tttgcccttt ttctgggctg acggtggtga aaatacagcg gctacaaggt
     7981 ttaaccacat caaacaccac atcaccaatg cgaatcactt tccagctatc ttcttcccag
     8041 gctgacgcgc cggaaaccac cagattgggg cggaattgct ccatttttac actggccgga
     8101 caacgttgtt ggagatcacg taacgaggct tcgttagcaa gaaggtaagg atagccatca
     8161 gcaaatgaca gaggtacagt gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc
     8221 cagcgtaatt gcacttcgcg ggagaaaaat ccacttagcc atttgttgat cgcgtctggc
     8281 gcaattcgcg cggtaaaatg tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa
     8341 tcagcaaaac gaacatatgc gctactgcca tctggtgcgg tgagatgcaa gccatcatgc
     8401 acaggtgaag gagtaaaccg taccatctgg ggaaactggc gagcggtaat aaacgtaccg
     8461 tcaggttccg tgatcataaa gatgcgatcg aaggccagac cactgacatc agccagagca
     8521 tgtgtaagac caatgccgcg catcgattta acaggatgaa taaaaagccg ggttaatgtc
     8581 gccacggtgc ggtcctcaaa tgaaaataag ccctcaactt tatgacatga cgcgcttatt
     8641 agctataatg cgcaacaatt ttcttagcgc ctgtcccgtt aggctatttt atttgtcagt
     8701 ttggccctgg gttgtgctcg aaatcctcac gtacttgtgt acgctccggt ttctccgcgc
     8761 tctccatgtc cagactgact gcaacaatta cgcctactgc ggtaggctct tagagtaaaa
     8821 gtgacgatat gaattctctg tttgccagta cggcccgtgg gctggaagag ctgttaaaaa
     8881 ctgaactgga aaatctgggg gccgttgaat gccaggtggt tcagggtggg gtccatttca
     8941 agggcgacac acggcttgtt taccagagcc tgatgtggag ccgcctggcc tcgcgtatta
     9001 tgttgccgct gggcgagtgt aaggtttaca gcgatttaga cctctatctc ggtgttcagg
     9061 cgatcaactg gacagagatg tttaatcctg gcgcgacctt cgctgtccac ttcagtggtt
     9121 tgaatgacac catccgcaac agtcagtacg gcgcgatgaa agtgaaagac gcgatcgtcg
     9181 atgctttcac gcggaaaaat ctgccgcgtc caaatgttga tcgcgatgcg ccggatatcc
     9241 gcgttaacgt ctggctgcat aaagaaaccg ccagtatcgc tcttgatctg agtggtgatg
     9301 gtttacatct gcgtggctat cgcgatcgtg ctggtattgc gccgatcaaa gaaaccctgg
     9361 cagccgctat tgtgatgcga tccggctggc agtcaggaac accgctgctc gatccgatgt
     9421 gtggttccgg tacgttgctg attgaagcgg cgatgctggc gaccgatcgc gcaccaggct
     9481 tgcaccgtgg gcgttggggc tttagcggct gggcgcagca tgatgaagct atctggcagg
     9541 aagtgaaagc ggaagcgcaa actcgcgccc gtaaaggcct ggctgagtat agctctcatt
     9601 tttacggttc ggacagcgac gcacgggtga ttcaacgtgc gcgcactaac gcccgtcttg
     9661 cggggattgg tgaactgatc acctttgagg tgaaagatgt cgcgcaactg accaatccgc
     9721 tgccgaaagg gccgtacggt acagtgttga gcaacccgcc atacggtgaa cgtctggaca
     9781 gcgaaccggc gctgattgcg ctgcatagtc tgctgggccg aatcatgaaa aaccagttcg
     9841 gtggctggaa tctctctttg tttagtgcct cgccggatct gctaagctgc ctgcagctgc
     9901 gtgcagacaa acagtacaag gcgaaaaacg gcccgctgga ctgcgtacag aaaaattacc
     9961 acgttgccga aagcacacca gacagtaaac cggcaatggt agcggaagat tacgccaacc
    10021 gcctgcgtaa gaacctcaaa aaattcgaga agtgggcacg ccaggaaggg attgaatgtt
    10081 accgcctgta tgacgccgat ctgccggaat ataacgttgc cgttgaccgt tatgccgact
    10141 gggtggtggt gcaggagtat gcgccgccaa aaactattga tgcgcacaaa gcgcgtcagc
    10201 gtctgttcga tattatcgct gcaaccattt cggtactggg gattgcgcca aacaaactgg
    10261 tgctgaaaac ccgtgaacgc cagaagggca aaaaccagta ccagaaactg ggcgagaagg
    10321 gcgagtttct tgaagttacc gaatataacg ctcacttgtg ggtgaacctg acggattatc
    10381 tcgatactgg tctgttcctc gatcaccgta tcgcccgccg tatgctcggt cagatgagca
    10441 aaggcaaaga tttcctcaac ctgttttctt ataccggcag cgccaccgtg catgcgggat
    10501 taggcggtgc acgcagcacc accaccgtgg atatgtcgcg tacttatctg gaatgggcag
    10561 aacgcaacct gcgtctgaat ggcctgacta ggcgtgcgca tcgcctgatt caggccgatt
    10621 gcctggcgtg gctgcgtgag gcaaatgaac agttcgatct gatctttatc gatccgccaa
    10681 ccttctctaa ctcaaaacga atggaagatg cgtttgatgt tcagcgcgat catctggcgc
    10741 tgatgaaaga tttgaaacgt ctgctgcgtg caggtgggac gatcatgttc tcgaacaaca
    10801 aacgtggctt ccgtatggat ctcgacggcc tggcaaaact gggactgaaa gcacaagaaa
    10861 ttacgcaaaa aacgctctcc caggatttcg cccgtaaccg ccagatccac aactgctggc
    10921 tgattaccgc agcctgaaag gaatagtaat gtcattaatc agtatgcatg gcgcatggct
    10981 gtcgttcagc gacgcgccgc ttctcgataa cgcagaactg catatcgaag ataacgaacg
    11041 tgtttgtctg gtgggccgca acggcgcagg aaaatcgacg ttaatgaaaa tcctcaaccg
    11101 tgaacaaggg ctggatgacg gtcgcattat ttatgagcaa gatttgattg tagctcgtct
    11161 gcaacaggat ccgccgcgta acgttgaggg tagcgtttat gatttcgttg ccgaaggcat
    11221 tgaagaacaa gcggaatatc tgaaacgcta tcacgatatt tcgtgcctgg tgatgaacga
    11281 cccgagcgag aaaaatctca acgaactggc gaaggttcag gaacagctgg atcaccacaa
    11341 cctgtggcag ctggaaaacc gcatcaacga agtgctggcg caactggggt tagatcctaa
    11401 cgttgcgctg tcgtcgcttt ccggcggctg gttgcgtaaa gcggcattag gacgcgcgct
    11461 ggtgagcaat ccgcgcgtat tgctgctgga cgaaccgact aaccatctgg atatcgaaac
    11521 catcgactgg ctggaggggt tccttaaaac cttcaatggg acgattattt tcatctccca
    11581 cgaccgttcg tttatccgca atatggcgac gcgcattgtt gatctcgatc gcggcaagct
    11641 ggtgacctat ccagggaatt acgaccagta cctgcaggaa aaagaagaag ccctgcgcgt
    11701 ggaagaacta caaaatgccg agttcgatct caaactggcg caggaagaag tgtggattcg
    11761 ctaggggatc aaagcacgcc gtacccgtaa tgaaggccgc gtacgcgccc tgaaagcgat
    11821 gcgtcgcgaa cgtggtgaac gtcgcgaagt gatgggtacc gcaaagatgc aggtggaaga
    11881 ggccagccgc tccggtaaga tcgttttcga aatggaagac gtttgctacc aggttaacgg
    11941 taagcaactg gtgaaagatt tttctgccca ggttctacgt ggcgacaaaa ttgccctgat
    12001 tggtccgaat gggtgcggca aaaccacgct gctgaaactg atgctcggtc agcttcaagc
    12061 gggcagcggg cgtattcacg ttggcaccaa actggaagtg gcttatttcg atcagcaccg
    12121 cgcggaactg gatcccgata aaacggtgat ggataacctt gccgaaggta agcaagaggt
    12181 gatggttaat ggcaagccac gccacgtatt gggctatttg caggactttc tgttccatcc
    12241 gaaacgggcg atgacgccag tacgtgcgct ttctggcggt gagcggaacc gcttgctgct
    12301 ggcgcgtttg ttcctcaaac caagcaactt attgattctt gacgaaccga ccaacgatct
    12361 tgatgtcgaa acgctggaac tgctggaaga actgatcgac agctatcagg gcacggtatt
    12421 gctggttagc cacgatcgtc agtttgtcga taacaccgtt acagaatgct ggatcttcga
    12481 aggcggcggt aaaattggtc gttatgttgg cggttatcat gatgcccgtg gtcagcaaga
    12541 acaatatgtg gcactcaaac agcctgcggt gaaaaaaagc gaagaagccg ccgcgccaaa
    12601 agcagaaact gtaaaacgca gcagtagcaa actaagctat aaattgcagc gcgaactgga
    12661 gcagctaccg caattgctcg aagatctgga ggcgaagctg gaagccctac agacgcaagt
    12721 ggcggatgct tccttcttca gtcagccgca tgagcagacg caaaaagtgc ttgctgatat
    12781 ggctgctgca gagcaggagc tggagcaagc ctttgaacgc tgggagtatc ttgaagcgtt
    12841 aaaaaatggt ggctgatcgc aaaatagtcg atttaaacag agacagctga caacggtaaa
    12901 attgcctgat gaactgggtt tatcaggcat gaaaaccaac actttgttag tcatctgtaa
    12961 aatagcgcat cattaaggag taccaatgtg cgaacatcat catgccgcga agcacatcct
    13021 gtgctcgcag tgtgacatgc tggtggcgtt accgcgcctt gagcatggtc agaaagcggc
    13081 atgtccccgg tgtggcacaa cgttaaccgt ggcgtgggat gcccctcggc agcgtcccac
    13141 cgcctatgcg ttggctgcac tgttcatgct gttgctgtcc aacttgtttc cttttgtgaa
    13201 tatgaacgtt gcaggagtta ccagtgaaat tacattactg gaaattcccg gcgtgctttt
    13261 ttctgaggac tacgccagcc tcggcacctt tttcctgttg tttgtgcaac tggttcccgc
    13321 gttttgtctg ataaccattc tgttactggt gaatcgcgcg gaattaccgg tccgtttaaa
    13381 agagcaactg gcacgggtgc tttttcaact caaaacctgg ggaatggcgg agattttcct
    13441 cgcgggtgtg ctggtcagtt tcgttaaact gatggcttac ggcagcattg gggtaggcag
    13501 cagctttctc ccctggtgtt tattttgtgt cctgcaactg cgcgcttttc agtgcgttga
    13561 tcgtcgctgg ttatgggacg acatcgcccc gatgccagaa ctgcgccagc cgctaaaacc
    13621 aggcgtcacg gggatacgtc aggggctgcg ttcgtgctcc tgttgtacgg caatccttcc
    13681 tgctgatgaa cccgtgtgcc cgcgttgtag taccaaaggg tacgttcggc gtagaaacag
    13741 cctgcagtgg acactcgcgc tgcttgtaac gtccatcatg ctgtatcttc cggctaatat
    13801 tttgcccatc atggtgacgg atttattagg ctcgaagatg ccgtcgacga ttctcgctgg
    13861 ggtcattctg ttatggagcg aaggctctta tcccgtcgct gtggtgatct ttctggccag
    13921 tattatggtg ccaacgttaa agatgagcgc catcgcgtgg ctgtgttggg atgccaaagg
    13981 gcatggcaag cgcgacagtg aaagaatgca tttgatttat gaagttgttg agtttgtagg
    14041 ccgctggtcg atgattgacg ttttcgttat cgcggtgctc tcggcgctgg tgcgtatggg
    14101 aggtttaatg agtatttatc cggcaatggg tgcattaatg tttgctttag tcgtcataat
    14161 gacaatgttt tctgctatga cgtttgaccc gcgtttgtcg tgggatcgtc aacctgaatc
    14221 agagcatgag gagtcctgac agtatggaat ctaataatgg ggaagccaaa atccagaaag
    14281 tgaagaactg gtctcccgtg tggatatttc ctatcgtcac ggcgctcatt ggggcctggg
    14341 ttctttttta tcattacagc catcagggac cggaagtgac cctgatcacc gcgaatgcgg
    14401 aaggaattga aggtggcaaa accaccatta aaagccgtag cgttgacgtc ggcgtggttg
    14461 aaagcgccac actggctgat gatttgacgc acgttgaaat caaagcgcgg ctgaattccg
    14521 gtatggaaaa attgctgcat aaagacaccg tcttttgggt ggtgaaaccg cagattggtc
    14581 gcgaagggat tagcggcctg ggaacgctgc tgtctggagt ttatatcgaa ctgcagccag
    14641 gcgcgaaagg cagcaaaatg gataaatacg atttgctgga ctcaccaccg ttggccccgc
    14701 ctgatgcgaa aggtatccgt gtgattctcg atagcaaaaa agccgggcag ctctcgccag
    14761 gagatccggt gctgttccgt ggctatcggg taggttcggt tgaaaccagc accttcgata
    14821 cgcaaaaacg caatatcagt tatcaactgt tcatcaatgc accttatgac cgactggtga
    14881 ccagcaatgt tcgcttctgg aaagatagtg gcattgcggt tgatctgacg tcagcgggga
    14941 tgcgtgtgga gatgggctca ttgacaacgc tgcttagtgg cggtgttagc tttgatgtgc
    15001 cggaaggtct ggatttaggg cagccagtgg caccgaaaac agctttcgtt ttgtatgatg
    15061 atcagaagag cattcaggat tcgttgtaca ccgatcacat tgattatctg atgttcttta
    15121 aagattcggt acgcggtctg caaccgggag ctccggtaga attccggggt attcgcctgg
    15181 gtaccgtaag caaagtgcca ttctttgcgc cgaatatgcg tcagacattt aacgatgatt
    15241 accgtattcc ggtactgatt cgtatcgagc cagagcggct gaaaatgcag cttggcgaaa
    15301 atgcggatgt tgttgagcac cttggcgaat tgttgaaacg tggtttacgc ggatcgctga
    15361 aaaccggaaa cctggtcact ggtgcactgt atgttgatct cgatttctat ccaaatacgc
    15421 ctgcaataac cggtattcgt gaatttaatg gttatcagat tatcccgact gttagcggcg
    15481 gcctggcgca aatccagcaa cgactgatgg aagcgttgga taagatcaac aaactgccat
    15541 tgaatccgat gattgaacag gcaaccagta cgctttctga aagtcagcgc acaatgaaaa
    15601 acctgcaaac gacgctggat agcatgaaca agatcctcgc cagccagtcg atgcagcagt
    15661 tgccgacgga tatgcagtca acgttgcgtg aattgaatcg cagcatgcag ggcttccagc
    15721 ctggctccgc agcctacaac aagatggtgg cggatatgca gcgccttgat caggtgttgc
    15781 gagaactgca accggtgctg aaaacgctca atgagaagag taacgcgctg gtatttgaag
    15841 cgaaggacaa aaaagatcca gagccgaaga gggcgaaaca atgaaaaagt ggctagtgac
    15901 gattgcagca ctgtggctgg ccggatgcag ctccggcgaa attaataaaa actattacca
    15961 gttacctgtg gtgcagagcg gtacacaaag taccgccagc cagggcaatc gtctgttatg
    16021 ggtagagcag gtcactgttc ctgactatct ggcggggaat ggtgtggttt atcaaaccag
    16081 tgatgtgaag tatgtgattg ccaacaacaa cttgtgggcc agcccgttgg atcaacagtt
    16141 gcgcaacacc ctggttgcca acctgagtac gcaactgccc ggctgggtgg ttgcctccca
    16201 gcctctggga agcgcccagg acacgctcaa tgttaccgta acggagttta acggtcgcta
    16261 tgatggcaag gtcattgtca gtggtgagtg gctgttgaac caccagggac aactgatcaa
    16321 acgtccgttc cgtctggaag gagtgcaaac tcaggatggt tacgatgaga tggttaaagt
    16381 gctggccggt gtctggagtc aggaagccgc ttctattgca caagagataa agcgtctacc
    16441 ttaattataa agatttgtaa atataaccgt ctccggtatg ccgcctgagg cggttttttt
    16501 gtttctaacg tgcggaaaaa tttgttcctc ttcacatttt ttgtacaacc gacatgcccg
    16561 tgtagctcac aaatatgaca gtggcgtgaa ttttgcgcat tgacggcagt tatgattcgc
    16621 ggtattgctt aactgtgatt gcacatttag taatcactgt tttcttttcc accagaaacc
    16681 agtatgaggg aaacgaggca tgaagagaca aaaacgagat cgcctggaac gggcacatca
    16741 acgtggttat caggccggca tcgccggacg ctcaaaagaa atgtgtccct atcagacgct
    16801 gaatcaaagg tcacaatggc tgggaggctg gcgagaagcc atggcggaca gggtagtaat
    16861 ggcctgattc tgtctcttta aaaagaaacc tccgcattgc ggaggtttcg ccttttgata
    16921 ctctgtctga tgataatcag aaggcagacg tatcctggaa cagaccgact ttcaggtcgc
    16981 tggcggtata gatcagacga caatcaacca gcacttcgcc atctgccaga cccataatca
    17041 gacgacgatt aacaatgcgt ttaaagtgaa tacggtaggt cacttttttc gctgtcggca
    17101 gtacctgacc agtgaatttc acttcgccaa cgcccagcgc gcggccttta ccttcgccgc
    17161 ccagccagcc gaggtagaac cctaccagct gccacattgc gtccaggccc aggcatcccg
    17221 gcataaccgg atcgccaatg aagtggcatc cgaagaacca cagatccgga ttgatatcca
    17281 gttctgcttc aacataccct ttgtcgaagt taccacccgt ttcggttatt ttgaccacac
    17341 ggtccatcat cagcatgttc ggtgctggca attgcgggcc tttagcgcca aacagttcac
    17401 cgtgaccaga ggcaagaagg tcttcttttg tataggattc gcgtttatct accatgttct
    17461 ctgtaagcct tattttattg aagcacgcag gatagctaac acgtgtacgc tgaacaagtc
    17521 cgatcagttc ggaataaacc agttcagcca acgtaatggc catggaaaac ggtgacgtcc
    17581 ttcctgttgc gatgcttgcg cgatacgttc ctggatggtt tgcatcagcg tcgtttggcc
    17641 ttcgccatcc cacaccagat ttaataataa cggcagtgcg tcagtcacat cgtctactgc
    17701 ccagatggtg aatttgcctt cttctaccgc tttcaccagt tcactgtgaa gacttaaatg
    17761 gcgaacgtta gcagtgggga taatgacacc ttgtttcccc gttaactcac gttgctggca
    17821 aatagcaaag aagccttcga ttttctcatt taaaccaccg accggctgga cgcggccgaa
    17881 ctgatcgact gaacctgtga tagcgatact ctgattcact ggcacatcag cgagggcgct
    17941 tatcagggcg cagagttcag ccatcgaggc actatcgcca tccacttcac tgtatgactg
    18001 ctcaaatgtc agcgatgctg agaaggggat ctgttgctca agctgtagtt ccgacatcag
    18061 gaacgcctgc atgatcatca tccctttcgc atggatattg ccgccaagct ccgctttgcg
    18121 ttcgatgtcg gtgaattcac catcgccaat atgcacaacg cagctaatgc gagaaggttc
    18181 gccaaaagcg cgtggatgac ccggaaattc aatgaccgaa agggcgttaa tttgcccgat
    18241 gcgttcgcct tcggtttcaa tcaggatttg ctcctgaagg atctcatcct gcatacgttc
    18301 agcgaggaaa ccttcgcgcc attcacgctg ctgcagcatt aagtttagct gctcgccgga
    18361 gaaggtgtcg ccatcacaca gggaggcgac ctctttacac tggcggagga tccaatgcgg
    18421 gctaagcgga agtgtttctt gttcaccggt gtagcgtgct gcttcgcgga taagtaccgg
    18481 ccaagcatcc gctcccgggg caggtaagtg attatgtctg gcggtaaatg tcacccagcg
    18541 acaccactgg cttactgact ccgcatcgac aatctgcaga gtatcttcaa attcgctata
    18601 aatagcctgc tctgaaagct ctggctccat ctcctggaaa tcagccaatg attcgcgttc
    18661 gcctaccaga atgactttca gcttcaatgg catcgaaggc acagagacgg ggagagggcg
    18721 cgactcatca aacgcaaccc agtcaaaacg ctcgcggtta acgatatttt tcagccgcat
    18781 ccacagcaga ggttgcgcca gcagtgtacg caaagagatg atgagaatac cgccatttgc
    18841 ctgatgcacc agaccaggct gcagggtaat gtcgccatta aactggcgca ggcagccaaa
    18901 gagttgctcc gcttctaccc agtcggcagc gacaacttgc gttaaagtcg caaaattatc
    18961 atctgcactc actgcgtgac gtaagcggat ggagtggccg gaaacctcat agtgatcgcc
    19021 caccagttgg cctgcatcgc tttgtagcgt acgcgcggca tcggcaataa gattcagata
    19081 ctcagactct tccggggcct tagccagcat aaaagaggag gatgctcgcg tatgcagcaa
    19141 ctgctccagc gcaaattgca gtcgcggttg agtatcactg aataaaggat cgttttcgtc
    19201 aatcaaatgt ggctgagcaa atatttcctg atagctatcg gtatcaggaa ccaggtcacg
    19261 ccatgcaagt ttcgtaatgg tcaaagttga tgttttttag tctgttgtca aagccgcgat
    19321 tataccgtaa ccggcactac agcacacgta gaaagcaccg acaatactcc tggctgggcg
    19381 ttaaagctca caggatggag attcttttct tcactggcct aaaaagctga tattctgtaa
    19441 agagttacac ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag
    19501 cggttggaaa tggaagtatc tggtaaagaa gcatcgcgaa ggggagttaa ttacccgtta
    19561 catagaagcc agtgccgccc aggaagccgt cgatgtgttg ctctcgctcg aaaatgaacc
    19621 cgtgctggta aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa
    19681 gcagaccatt cgggccagac ggaagcgcca ttttaatgcg gaacaccagc atacgcgcaa
    19741 aaaatcgatc gatctggaat ttattgtctg gcaacgtctg gctggtcttg cacagcgtcg
    19801 cggtaaaacg ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa
    19861 atacgcgaat aaaatgtctt ctctgaagca ggatctgcag gcattgctgg gtaaggaata
    19921 actgacgaaa gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca gcggggtttt
    19981 tctaccagac gataacttaa gcctgcggct gagttacaac gtctttgata cctttaactt
    20041 cgatctctac gcgacgatcc ggagccaagc agtcgatcag tgcagcacgc tgtttcacgt
    20101 tgtcacaggt gttgccagta accgggttgg attcgcccat accacgtgcg gagatcttgt
    20161 ctgccgggat acctttggag atcaggtaat caacaacaga ctgagcacgg cgctcggaca
    20221 gaccctggtt gtaagcgtca gaaccgatgc ggtcagtgta acccagaaca actacggaac
    20281 cgtctttcgg atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt
    20341 ccggtttcag ggttgctttg ttgaagttga acagaacgtc agacttcaga gtgaagtgct
    20401 tggtctgtac ttccggtgcc ggagctggag caactaccgg agctgcttcg ccctgaccga
    20461 aacggtagga aacacccaag ctcagcagac cgttgtccgg acgagtaccg atggtgtttg
    20521 cgtcaccgat gttgttggtc cactggtatt ccagacgggt agcgatttca ggagtgatcg
    20581 cgtactcaac accacctgcg aaaaccggag aaacgccagt gtcgtggtct ttaaaggatg
    20641 cgccaccagg tacgttagcc ttggtgtctg cacgccatac cataccaccc agacgagtgt
    20701 agatgtccag atcgtcagtg attgggtaac ccagtttagc ggtcagctga acgccctgag
    20761 ctttgtatgc gccgttgatg ttgtcgcctt tgtacggcat acgacctaac cagtcgtaac
    20821 ccatttcaaa accaacatac gggttaacct ggtaaccacc aaaagcacct gcacccagtt
    20881 ggttttcgtg ggtcggacca ttgttaggaa taaaaccagt gtcatggtac tgggaccagc
    20941 ccagtttagc accagtgtac caggtgttat ctttcggagc ggcctgcgct acggtagcga
    21001 aaccagccag tgccactgca atcgcgatag ctgtcttttt cattttttgc gcctcgttat
    21061 catccaaaat acgccatgaa tatctccaac gagataacac ggttaaatcc ttcaccgggg
    21121 gatctgctca atattaactc taccgatatc ttcggcttat gccgagcacc cctggcgatg
    21181 taaagtctac aacgtagttg aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa
    21241 aagtcttgta taaggtatgt ttaatctttt tgtcagcgac aatttacaga aaaaaaacgc
    21301 ggaaaccgct tcagacaagc cttcgcaagg aaaattagtc acgactgaaa gcattggctg
    21361 ggcgacaaaa aaagttccag gattaatcct aaatttactt aatgatacaa attagagtga
    21421 atttttagcc cggaaagttg tctcgttgtg tgagaggatg cgcttaccgg acgcataata
    21481 aaccccatag cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc
    21541 aaatcatctg tcaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc
    21601 attgactcca ctgtgtggca aggggagagc tggctaatct gcattacttt cgttaagggt
    21661 agcccagatg cctgaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc
    21721 cagcgcgatt gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg
    21781 ggctgatctt cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag
    21841 acacgcgcaa ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa
    21901 gtgtacataa tcaatccagc ccctgtgagt tactgtatgg atgtgcagta catccagtga
    21961 caacaaagat caaccctatt ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga
    22021 aaacataaat taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc
    22081 gttaatgtga atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc
    22141 ctataagcgg atctataaat cacaagaata cctggcaacg ttgggcacaa ttgaataccg
    22201 atcattgttt ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga tggtttctga
    22261 tggtgagttg tatcttcggg cttgtgagca aagtgcacag tactgtgtaa aacatccgcc
    22321 tgtctggctg acatataaaa agtgtggccg atccgttacc ctcaattact atcgggttga
    22381 tgaaagtcta tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc
    22441 agcgctgaaa gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat
    22501 gtcttttcat ctggaagcga ttctcgggga ggtggggatt aaggatgtac gggcgttacg
    22561 tatacttggg gcaaaaatgt gttggttgcg actgcggcag caaaacagtc tggtgacaga
    22621 aaagattctg tttatgcttg aaggtgccat tatcggcatt catgaagctg cgctcccggt
    22681 ggcacgccgc caggagcttg cagaatgggc tgactctctt acgccgaaac aggagtttcc
    22741 tgcggagctt gagtaatctg gcgttgcaga cgaccaatct ctggcagtaa tgcaatcaat
    22801 aacccaactt gttgtacgac cagaggttct ttgctatctg cccgtggttc aagttgctgc
    22861 atccgctgtt tcaggccagc taatgcctga ttgacgcgtt cttcatcagc aggttgatga
    22921 tgtaacgcgt catcaacata gcaaactgcg tcatcaagaa acgccagaat ttcaggatta
    22981 gttaactgct cccggtgagc accgagggct gagatatagc tggtaaacgt atggttaagg
    23041 cacagcaagc gaaacgcggc ttcgcgaatt tgcggggtaa cgttcggttc gctggacata
    23101 tttgatacca cagacgccag ttcagcatca cggttgtgtg catcgcggcg ggcaatacga
    23161 tacgccagac ggttatcacg cccctgatgg tattgctcca gtatggcatc gagatagcga
    23221 cagttggcct ctgtggcgcg ttcgagcatt cgcggcagat tgcgaaactt ccagtcgggc
    23281 cagatgtagc tcactgccgc ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga
    23341 ggtaacgcta cttcaaaacc ttcacccagt aagttaaaac acagtagcac caaaagtgtg
    23401 atgaacatcg ttgcatgagc gtattgcacg ttacggaagg caaaaaagag cacgccggta
    23461 ataaccagca gcaccagctg cccttccagt gatggcacaa accacagcac aggaatgcca
    23521 atcgcgatac ctaccagcgt accaataatc cttaacttca ggcggtggcg cgtggcgtta
    23581 tagtttggct ggcagacaaa caaacttgtc agcaagatcc aatacccgtg atgcattccg
    23641 gttatttgaa tgatggcgta gccgaagcac aacaccagcg acattcttac cgcatgacgg
    23701 aagagggcgg attccggcgt gaagtgacgg ctaagacgca gccagatatc actcaatccg
    23761 tgcgggctgt catcagcgag ctcattttcg tcattattac ggggtagtgc ctgggcctgt
    23821 tctgattcaa ttgttgccag ttgggcatca atggcgcgta aattgttcag caaaaatccc
    23881 agtgttttga gtaaatcggc gggtgcgccg ttatcgcgta tccgctccag cgcagcatca
    23941 atatgcgtaa aagcgcgctc aaaatgcgga tcatgttgat aagactgacg caacaaaata
    24001 cagcgtgaca gttgctggca cgcctggccc tgcatcgaca tcagccgctg aaaacggaac
    24061 agcacgtcgc tgtggcgaaa atgttcacgc aatgtttgat actgaatatg agaagagctg
    24121 gcacgctcgt gaatatcctg tgcgacaaag taataatgca gcgtgcgacg cgttccccgt
    24181 tgaccacgat cgccacgtaa gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc
    24241 atcagctgac cgttggcgag agccaaatcg tacagcggtg cctggctttc atcttcaata
    24301 tcaggatcaa acatgcgcga cttgagctca agataacgcg ccagttgttc atagcaacgc
    24361 gccaggttgt cctgcagcgg gcggaccggg aacagcagat gaccaataag tgtcaggacg
    24421 ttgtaccaga cggcaccggc cagcagatac atcggctgct gataccagtg ctcatacagt
    24481 gatgttccca acatagtgta aatggcgatc agcaatgcac cgaaggcaat tgttgcatag
    24541 cgttgaccca gaccgccgag caaaatgaag ccgctggtag agagcgttaa gccaatcgca
    24601 aatagccagg gccagggaaa cagcaattct actgaggccg aggcgataaa aaagcagaac
    24661 agcgtaatga tgaggttacg caaacgtccc gccagtcggt catcgagatc ggtcagcgcc
    24721 gctgccacca tccccagcgt tagcggaatc gtcagtttta catcacccag ccaccacgga
    24781 aacgctgtgg ttccacaaag cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc
    24841 caagtatagc gtttgagcaa aggacttagc ataaaggcca tattagtgga ttattcaaaa
    24901 cgacgacgtg cattggcttc acgcgcagct tgcgctgttt ctaccgatac cacgcgacga
    24961 ccaaccggcc atagtgcaat agcggcaatt ttaaagttcg caatgccgac aggaatgcca
    25021 atgattgaaa tacattgtgc gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca
    25081 aagaaaatca gccagaaaat attcaatacc gtaccgccag tattcagcaa cacatttttg
    25141 ccagccgggt tcagttcatc gacatgaata gcttcattgc cataaggcac cagagacagt
    25201 ttagtgatct cccagcagga tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc
    25261 agagtcgcca acagccagcc cagagtggtg gcaaatccgc caagcacaaa gttcagaatg
    25321 ttcaaaacgg tacgcataaa acctcgcttt actgtggttt tcagtaatgg gcggcaattg
    25381 taacgttttt ttgggctgga gcacgttttc tctgacggtt acactgataa gaaataattt
    25441 cgtgtggatc tacagagtca tggaactgaa agcgacaacg cttggaaaac gtctggcaca
    25501 gcacccttac gatcgggcgg tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca
    25561 cgaatacctt attcctttca atcaattact ggcgattcac tgtaagcgcg gtctggtatg
    25621 gggcgagctg gaatttgtac tgccggacga aaaagtggtg cgtctgcacg gcacagaatg
    25681 gggcgagacg cagcgttttt accatcatct tgatgctcac tggcggcggt ggagtggcga
    25741 gatgagcgaa attgcgtctg gtgttttacg ccagcaactg gatttgattg ccacgcgcac
    25801 tggagaaaat aaatggctga cgcgtgagca aacctctgac gttcagcaac aaatccgcca
    25861 ggctttgtcg gcgttgccgt tgccggttaa ccgactggaa gaattcgata actgccgtga
    25921 ggcgtggcgt aaatgtcagg cttggttgaa agatattgaa agcgctcggt tgcagcataa
    25981 ccaggcgtat accgaagcca tgcttaccga gtatgcggat tttttccgcc aggtcgagtc
    26041 ttcaccgctg aatccggcgc aggcccgggc agtcgttaat ggcgagcatt ctctgttagt
    26101 gctggccggt gcaggaagcg gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct
    26161 ggcgcgtggt gaagcgtccc ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc
    26221 tgaagagatg gacgagcgga ttcgcgaacg gctacatacc gaagacatta ccgcacgcac
    26281 gtttcatgcg ctggcgctgc atattattca gcagggcagc aaaaaagttc cgatagtcag
    26341 caaactggaa aatgataccg ctgcccgtca tgaactcttt attgctgagt ggcgcaagca
    26401 atgcagcgaa aagaaagcgc aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca
    26461 gtggtcagtg ccagaaggta acttctggga tgatgaaaaa ttacagcgtc gccttgcctc
    26521 tcgcctcgat cgctgggtaa gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat
    26581 tgccagtgca cccgaagaga ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc
    26641 gttattaaaa gcctggaaag gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct
    26701 tattcatcag gcgattgtga ttctggagaa aggccgcttt atcagcccgt ggaagcatat
    26761 tctggttgat gaatttcagg atatctcgcc gcagcgcgca gcgttgttag cggcattacg
    26821 caagcaaaac agtcagacga cgttgttcgc cgttggtgat gactggcagg cgatttaccg
    26881 attcagcggt gcgcaaatgt cgctcaccac cgctttccat gaaaactttg gtgaaggcga
    26941 tcgctgtgat ttagacacga cttaccgttt taacagtcgt atcggtgagg tggcaaaccg
    27001 gtttattcag cagaacccag gccagctgaa aaagccgcta aacagcttaa ccaatggaga
    27061 caaaaaagct gtcacgttat tggatgagag tcaacttgac gctttgctgg ataagctctc
    27121 tggttatgcc aaaccggaag agcgcattct gatcctggcg cgttaccatc acatgaggcc
    27181 tgccagcctg gaaaaagcgg caacacgctg gccgaagttg caaatcgact ttatgaccat
    27241 tcatgccagc aaagggcaac aggcggatta cgtcatcatc gttggcttgc aggaggggag
    27301 tgatggtttt ccggctgcgg cgcgggagtc gattatggaa gaggcgctac tgccaccggt
    27361 tgaggatttc ccggacgctg aagaacggcg tttaatgtac gtggcgctga cccgggcacg
    27421 ccatcgggta tgggcactgt ttaacaaaga gaatccctct ccctttgtgg aaatactgaa
    27481 aaatctggat gtgccggtgg cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg
    27541 caatattact tcagacggtc agcgagataa cgctgataat cggggataag aatatcgacc
    27601 gcgtcgttga aatgcggcga ctggataatg aagtctgccg ttgccacgtt ggtggcgacc
    27661 ggaatgttcc ataccgtcgc cagacgcagc aaggctttca cgtcaggatc gtgcggcacg
    27721 gcatttagtg gatcccagaa gaaaatcaat acatcaattt tcccttctga gatcaatgcg
    27781 ccaacctgct ggtcaccccc cattgggcca ctcaacatcg cgttgacgtt catgccggtc
    27841 gcgcgggaaa tcaagttacc ggtagtgcct gttgcataca gtacgtgttg ttccagtaac
    27901 ggttgatgcc gttccaccca gctcatcagc atttgtttgc agtgatcgtg tgccaccagc
    27961 gcaatatgtt tccgcgcagg taaagtgcga gtcgtcagtt ccataatgta catccgtagt
    28021 taactttcct acagattact gtaagcactt atcgctgcaa gataaagacc gaaaaagcct
    28081 gcgcacaggc acaaaaatct caggaagatg gttgtttttc cgcccattgc aggaaagtat
    28141 ttcgcgtttg tgggtcagcc agtttaaacc aatacttcag ccgttgttct gtgagcacct
    28201 gagactgcgg tggaatagca tccagctcgg aaacacccga aagcagcgta ctgtcatctg
    28261 ccatcatcgt ggcaaattgc ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa
    28321 cctcgacctc ataatcaatc gtttttgccg ttgaggtaat ggcgagaata tccagcttta
    28381 ccggaatcgg cgtcgcatcg ccatccaaca attcaaggcg cggcgcggca tcaaaatggt
    28441 tagcttcccg ctcattctcc aggcgaggca ggcgaaaatt gacctggttg atgagctggg
    28501 tattaaaact gaccaccagc ggtggggaga tatacagccg ttcttcacta ttggaaagat
    28561 gaatcgtctt ctcaacgcga aacactaact gatgcggtcc attgtccagt tcaatgctgt
    28621 ccgcaccgcg cagcagagag ctggaaacct ttttgccgtc gagtaccaga agatcgacat
    28681 cggttgaaag ccgcagcgtg gtggcaaaaa cggataccgg caaacataat gcaatcaagg
    28741 tggtcacgat gccggttttc ataggtagct cctgtcaaaa gaccgcccga ataataatgt
    28801 gtcaaaattt ttctttaatc atacttacta acatatagac atatttcccg cttttgctct
    28861 cattcattcg tattagctgc atggttggca tgtcggcttt gtcgtacact ttgttaaaca
    28921 gtctggagga aataatgaaa gaaaccgata ttgcaggcat tttaacgtcc actcacacca
    28981 ttgcgctggt gggggcaagc gacaaacccg atcgccccag ctatcgcgtg atgaaatatc
    29041 tactcgacca ggggtatcac gtcattccgg tttcgccaaa agttgccggc aaaacgctgc
    29101 tgggccagca ggggtatggc acgctggcag acgttccgga aaaggtcgat atggtcgatg
    29161 ttttccgtaa ttcagaagcg gcgtggggcg tggcacagga agctattgcc attggcgcga
    29221 aaaccctctg gatgcaactg ggggtgatta atgagcaagc ggcagtactg gcacgggatg
    29281 ccgggttaaa tgtggtgatg gatcgctgcc cggctattga gatccctcgc ctgggtctgg
    29341 ccaaataaaa aatccccgga aggcaaaaac cttccgggga tttgttcagg gattagttac
    29401 gcagacgcgg ggcctggagt tgtttgcgga tggtctgcgc cagctcatcc atggatggct
    29461 gttcaggatg ttcatcctgc aattcgctac ttagctgggc ttcagccagg taggtatgaa
    29521 ccggtaggcc gttatcgtcc tccatcacca cgtgatacca cggagcggcg cgaagctcgt
    29581 cattcaccgc cagctcatca ggcgacggtt cagagagcga ataaaccggg tcgatatcca
    29641 cgaccactcc gagataacct aacagggaat ggcggacctg ctggccgata ccgaatttgc
    29701 tggcaatcat agtcacctcc cgggaaatct ttacctttga tataagggta aaattccaca
    29761 tttcaagtta catgacgcga caggcaaacc ctttcagata tagcccttcc gggtaggtag
    29821 cgatcaccgg atgatcggct gcctgacgga actgctctat aaattgtaca tcacgaccgg
    29881 catcaattgc ggcatccgcg atgattttct gaaataaatc gctggtcatc agaccggaac
    29941 aggagaaagt caggagaata ccgccttcat tcagcaactg aatcgccagc atgttgatgt
    30001 ctttataacc acgacacgcg cccatcaact ggcttttatt ctcaacaaac ttcggcgggt
    30061 ccatcacgat aacgtcaaat ttttcaccgc gatcgcgata agtacgcagc aatttaaaga
    30121 catcatcacg gacaaactca gccttgctca gatccagttt gttcagctca acgttctgcc
    30181 gtgcaatatc cagcgcttcc tgggaggtat caacgctgac aacctggctg caaccgccca
    30241 tcagtgccga tacggcgaaa ccaccggtat aggagaaaca gttcagcaca cgtttatttt
    30301 caacgtagcg gcgggtagcc aggcggctgt ctcgctggtc cagatagtag cccgttttgt
    30361 gtccgtgctg aatatccacc agcagcttca tgccgtgttc ttcaatcggc agcagggcag
    30421 gcggcaactc gccggtgacg aggccctggg tcagctccat cccttctttt ttacgtaccg
    30481 caacatcgct gcgatcgtaa atcgcacatt ccgggtacag cgtttgcagg gcactaatta
    30541 atgccgcgcg ctgatattct gcgccagcac tcagcagttg cagcaccaga aaattaccga
    30601 aacgatcgat agtaataccc ggcaggccat cagattctcc ggcgattaaa cgatagctgt
    30661 cgaggccatc tttttgcgcc agccagtcac gccatttttg tgcttgttgc aaacggcggg
    30721 aaaaaaaagc aatgtcgata gactcagacg ggtcaaacgt ccagacgcgc gcccggattt
    30781 gcgaagctgg cgaataagcg ccgcgtgcta accattttcc ctgatgatca acaatatcga
    30841 tggtttcacc gaggctggct ttaccttcca tgcgggcaac ggccccggaa aagacccacg
    30901 gatggcgacg aagtaatgat ttttcgcgcc ctttggctaa cactaaacgt acactcataa
    30961 tttacttttc tgtcgatgca aagaaatggg cgtcattgtc cggagttttc catggatttg
    31021 caacgcgtcc attaaggata aggagagcag caatgtcgaa agtctgcata attgcctgga
    31081 tttacgggcg agttcagggc gtaggatttc gctacaccac acagtacgaa gcgaaaagac
    31141 tgggcttaac cgggtacgcc aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg
    31201 gtgaagaagg gcaggtagaa aaattaatgc agtggttaaa gagtggcggc ccgcgttctg
    31261 cgcgtgtaga acgggtgctc agcgagccgc atcatccctc gggggaatta accgattttc
    31321 gtatccgtta aatacatttt accggttttg gcaggccagc aattttggtg gcttgcttag
    31381 ccgggccttt cgggaacagt cggtataaat agcggctgtt acctttttct tcaccaaatt
    31441 tattcgccat cgcttttacc agcatacgaa tcgccggaga cgtattaaat tccaggtaga
    31501 agtcacgtac aaaacgcacc acttcccagt gttctggcga cagcgaaatc ccttcgttct
    31561 ctgcaatcac cactgccagc ggttcactcc actggctgct ttctttgaga tagccttcgg
    31621 tatccgtttc tatctcttta ccttcgaaga tcagcataat tattactacc ttaatcagac
    31681 tggcggcagt gtaacaaaaa acaaagcccc gcataaagcg aggctatgaa agtgttagcg
    31741 ggtgagatta atcgcggctg gcgaagccca gaatgctcag caggctgacg aagatgttgt
    31801 acagcgaaac atacaggcta accgtggcac gaatatagtt cgtttcaccg ccatgaatga
    31861 tgttgctggt ttcaaacaag atagcgccag aggagatcag aatgaagacc gcgctgatcg
    31921 ccagatgcag agcaggcagt tgcaggaaga tattagcaac cataccaatc agtaccacca
    31981 caatacccgc catcagcata ccgccgagga acgacatatc tttgcgggtg gtcagcacat
    32041 atgcagagca gcagaagaac actaacgccg ttccgcccag tgccatagcg attacgtcac
    32101 ccattccggc agacagatag gtgttcagaa ttggtccgag gatataaccc agaaaaccgg
    32161 taaaggcgaa tgcggagata atcccggtcg gcttattcgc cgttttatag gtcaggaaca
    32221 tcaaaccata catacccacc agcgtcagaa tcagacccgg agatggcagc atcagcaccg
    32281 tactggcagt tgcggtaatc gccgaaaagg ccagcgtcag gctcagcaga aaataggtat
    32341 tacgcagcac cttatgggtg ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac
    32401 gatccattga gtcactctct atgacagatg taattaatta agcagcataa tgataatgcg
    32461 taagggcacc cagaagtttt acccatcttt acgcatttga tctggaacag gtttaacagc
    32521 ggattatcag gtcattaagc aaatataacg ccctgagaat tccgacaggc aaaagaaaaa
    32581 ggggttagca tttagctaac cccttatctt atttggcgga agcgcagaga ttcgaactct
    32641 ggaacccttt cgggtcgccg gttttcaaga ccggtgcctt caaccgctcg gccacacttc
    32701 cggaatgacg cgcactataa acatcccgat gcggcgtgta aacccctaat ttgtttgttt
    32761 gcctgaaaaa cagccaaaag tgcattgata gcgtgaaata acagcagatt gatcatttca
    32821 tcaccatgaa ttccttctct tttactcgtt tagcaaccgg ctaaacatcc ccatcgcccg
    32881 gctaaagaaa aataggtcca tttttatcgc taaaagataa atccacacag tttgtgttgt
    32941 tttgtgcaaa agtttcacta cgctttatta acaatacttt ctggcgacgt gtgccagtgc
    33001 agaaggatga gctttcgttt tcagcatctc acgtgaagcg atggtttgcc ttgctacagg
    33061 gacgtcgctt gccgaccata agcgcccggt gtcctgccgg tgttgctagg agaagagacg
    33121 tgcgatatga ataacgagga aacattttac caggccatgc ggcgtcaggg cgttacccgg
    33181 cgcagctttc tcaaatattg tagtctggct gccacgtcgc tgggattagg cgcgggaatg
    33241 gcaccaaaga ttgcctgggc gctggagaac aaaccgcgca ttccggtggt atggatccac
    33301 ggtctggaat gtacctgctg taccgaatct tttatccgct ccgctcaccc actgacgaag
    33361 gacgtcatcc tttccctgat ttccctctat tacgacgata ccctgatggc tgccgccgga
    33421 acccaggcgg aagaagtctt tgaagacatc atcacgcaat ataatggcaa atatatcctc
    33481 gcagtagaag gtaatccgcc gctgggcgag caggggatgt tctgtatcag cagcggtcga
    33541 ccgtttattg agaaactcaa acgtgccgct gccggagcca gcgcgattat cgcctgggga
    33601 acctgcgcgt cctggggctg cgtgcaggcc gcgcgaccta atccgacaca ggcaacgcct
    33661 atcgacaaag tcatcaccga caaacccatt atcaaagtac ctagctgccc gccgatcccg
    33721 gatgtgatga gcgccatcat tacttacatg gtgacctttg atcgcttgcc agatgtcgac
    33781 agaatgggtc gtccactgat gttttatggt cagcgtatcc acgataaatg ctatcgccgc
    33841 gctcacttcg acgccggaga gttcgtccag agttgggatg atgacgctgc ccgcaaaggt
    33901 tattgcctgt acaaaatggg ctgcaaaggg cctaccacct ataacgcctg ttcctccaca
    33961 cgctggaatg atggcgtttc tttcccaatc cagtctggtc acggctgcct gggctgtgcg
    34021 gaaaatggtt tctgggatcg cggttcgttc tacagccgcg tggtcgatat tccgcaaatg
    34081 ggtactcatt ccaccgccga taccgtcggt ttaaccgcgc ttggcgtggt ggcagcagct
    34141 gttggtgtgc acgcagttgc cagcgccgtt gaccaacgca gacgtcataa ccagcaacct
    34201 acagaaaccg aacatcagcc aggcaatgag gataaacagg catgagcact cagtacgaaa
    34261 ctcagggata caccatcaat aatgccggac gccgcctggt ggtcgacccg attacgcgca
    34321 tcgaaggcca catgcgctgc gaagtgaata ttaacgatca gaatgtgatc accaatgccg
    34381 tctcctgcgg caccatgttt cgcgggctgg agatcatcct gcaagggcgc gacccgcgcg
    34441 atgcgtgggc gttcgttgaa cgtatctgcg gcgtctgtac tggcgtacac gccctggctt
    34501 cggtttacgc catcgaagat gccatcggta ttaaagtgcc ggacaacgcc aatatcatcc
    34561 gcaacattat gctgacaacg ctctggtgcc acgatcatct ggtgcacttc tatcagcttg
    34621 ccgggatgga ctggatcgac gtgttagatg cgctgaaagc cgacccgcga aaaacctccg
    34681 aactggcgca aagtctctcc tcttggccga aatcatcccc tagctatttc ttcgacgtac
    34741 aaaatcgcct gaagaaattc gttgaaggcg ggcagttggg gatcttccgc aatggctact
    34801 gggggcaccc gcagtacaaa ttgccgctag aagccaacct gatgggcttt gcccactatc
    34861 tcgaagctct cgatttccaa cgtgaaattg tcaaaattca cgcggtcttt ggcggtaaaa
    34921 acccgcatcc aaactggatt gtcggcggga tgccttgcgc cattaacatt gacgaaagcg
    34981 gcgcggtcgg ggcggtcaat atggaacgcc tgaacctggt gcagtcgatt atcacccgca
    35041 cggcagactt cattaacaac gtgatgatcc ccgacgcctt agccatcggt cagttcaaca
    35101 aaccgtggag cgaaatcggc acgggtcttt ctgataaatg tgtcctcagc tacggcgcat
    35161 tcccggatat tgccaacgac tttggtgaga aaagtctgct gatgcctggc ggcgcggtga
    35221 ttaacggcga cttcaacaat gtgctgccag tggatttggt tgatccgcag caggtgcagg
    35281 agtttgtcga tcacgcctgg tatcgttatc ccaacgatca ggtcgggcgt catccattcg
    35341 atggcattac cgacccgtgg tacaaccccg gcgatgtcaa aggcagcgat accaacattc
    35401 agcagctgaa tgaacaggaa cgctactcgt ggatcaaagc gccacgctgg cgcggtaacg
    35461 cgatggaagt ggggccgctg gcacgcacgt taatcgctta tcacaaaggc gatgctgcga
    35521 ccgttgagtc ggtcgatcgc atgatgtcgg cgttgaacct gccgctttcc ggtatccagt
    35581 caacgttggg ccgcaggtaa gttgcagtat ttcttcgaca agctgatgac caacctgaaa
    35641 aatggcaatc tcgccactgc ttccactgaa aaatgggaac ctgcaacctg gccgacagag
    35701 tgccgtggcg tcggctttac cgaagcgccg cgcggagcgt taggccactg ggccgccatt
    35761 cgcgatggca agattgatct ctaccagtgc gtggtgccga ccacctggaa cgccagcccg
    35821 cgcgatccca aagggcagat tggcgcttat gaagcggcgc tgatgaacac caaaatggcg
    35881 atccccgagc aaccgctgga gatcctgcgt actctgcaca gctttgatcc gtgcctcgcc
    35941 tgttcaactc atgtgctggg cgacgacggt agcgagctga tctccgtgca ggtgcgttaa
    36001 cagcgaagga gaatcatcat gcaacagaaa agcgacaaca ttgtcagcca ctatgtcttt
    36061 gaagcaccag ttcgtatctg gcactggctg acggtgttat gcatggcggt gttgatggtc
    36121 accggttact ttatcggcaa gccgctacct tccgtcagtg gcgaggcgac gtatctgttc
    36181 tatatgggtt acatcaggtt aattcacttc agcgccggga tgatttttac cgtggtcttg
    36241 ctgatgcgga tctactgggc ttttgttggc aatcgttact cccgcgagct gtttatcgtg
    36301 ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg gtatctgttt
    36361 ctggcaaaac gtccgagtgc cgatataggc cataacccca tcgcccaggc ggcgatgttc
    36421 ggctatttcc tgatgtcggt ctttatgatc atcaccggtt ttgcgctgta cagcgaacac
    36481 agccagtacg ctatttttgc gccgttccgt tatgtggtgg aatttttcta ctggacgggt
    36541 ggcaactcaa tggacattca cagctggcat cggctgggga tgtggctgat tggggcgttt
    36601 gtgatcggtc atgtctacat ggcgctgcgt gaagacatca tgtccgacga cacggtgatc
    36661 tccaccatgg tcaacggcta ccgtagccac aaatttggca aaataagtaa caaggagcgt
    36721 tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa cctgctgtgg gccgatgaag
    36781 gcttcggcgt gcgggtggcg gaacggctgt atgcccatta ccactggccc gagtatgtgg
    36841 agattgtcga tggcggtact cagggactga acttgctggg gtatgtcgaa agcgccagcc
    36901 atctgttgat tctcgatgcc attgactacg ggctggaacc tggaaggctg cgaacctatg
    36961 ccggagaacg cattccggcc tatctcagcg cgaagaaaat gagcctgcat cagaacagtt
    37021 tctccgaagt gctggcgctg gcagatatcc gcggacatct gccagcacat attgccctcg
    37081 tcgggctgca acccgcgatg ctcgacgact acggcggcag cctgagcgaa ctggcacggg
    37141 agcaactgcc cgctgcggaa caatcggcgc tggcgcagtt ggctgcgtgg ggaagtgtgc
    37201 cgcaaccggc taatgaatcg cgttgtctca attatgactg tctgtcgatg gaaaattacg
    37261 aaggcgttcg cttgcgccag taccggatga cacaggagga gcagggatga gcaacgacac
    37321 gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc tggacgccgg tcagtgaatc
    37381 ccgtcttgac gactggctta cgcaagcgcc agacggcgtg gtgttattaa gcagtgaccc
    37441 gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt ggcgaattac tgcacgagtt
    37501 tcccgactat acatggcagg tggcgattgc tgaccttgag cagagcgaag ccatcggcga
    37561 tcgctttggc gcctttcgct ttcctgccac tttagtgttt accggcggaa actatcgcgg
    37621 cgtgctgaat ggtattcacc cgtgggcgga actgataaac ctgatgcgcg ggcttgtcga
    37681 accgcagcag gagcgtgcct catgagcgaa acttttttcc atctgctggg gccaggaacg
    37741 caaccgaacg atgacagttt cagcatgaat ccactgccga tcacctgtca ggtgaatgat
    37801 gaaccgagta tggcggcctt ggagcaatgt gctcacagcc cgcaggtgat tgcgctgtta
    37861 aacgagttac aacatcaact aagcgaacgc cagccgccgt tgggcgaggt gctggcagtc
    37921 gatctgttaa atctcaacgc cgacgatcgt cactttatca atacgcttct cggggaaggg
    37981 gaagtgtcag tgcgcattca gcaggctgac gacagtaaaa gtgaaataca ggaggcgatc
    38041 ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga ggacaaactg
    38101 gaggctggct gcgcgccgct ggcgttgtgg caggcggcaa cgcaaaatct cttgccgaca
    38161 gattcgctgt taccgccgcc cattgatggc ctgatgaatg gcctaccgtt ggcgcatgag
    38221 ttactggcac atgtacgtaa ccccgacgcg cagccgcaca gcattaatct gacgcaatta
    38281 cccatcagcg aggctgatcg gctttttctc tcacgtctct gtgggccggg aaatattcag
    38341 attcgtacca ttggctatgg cgagagctat atcaactcca cggggttacg ccatgtctgg
    38401 catttacgct gtacggacac cttaaaaggc ccgttactgg aaagttatga aatctgccca
    38461 ataccggaag tggtgctggc agcgccagaa gatttggtcg actctgcgca gcggcttagc
    38521 gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa aagacggtaa gtatcgcttt
    38581 cagtcttatg aatatcgcaa tcggcgaata cctctggtcg tagagtttca ggataaagag
    38641 ggagatctaa cattatcggg ttatttttct ctcttcgcct acaggagtgc gcatgtggga
    38701 tgtcattgat ttatcgcgct ggcagtttgc tctgaccgcg ctgtatcact ttttatttgt
    38761 accccttacc ctggggctga tttttttgct ggctatcatg gaaaccattt acgtggtcac
    38821 cggcaaaaca atctaccgcg atatgacgcg cttctggggt aagctcttcg gtatcaattt
    38881 tgctcttggc gtggctaccg gcctgaccat ggagtttcag tttggtacta actggtcatt
    38941 ctattccaac tatgtgggcg atatttttgg cgcaccactg gcgatggaag cattaatggc
    39001 cttcttcctc gaatccacct ttgtcgggct gttcttcttc ggctggcaac ggctgaataa
    39061 ataccagcac ctgctggtga cgtggctggt ggcgttcggt tcaaatctct ctgcattgtg
    39121 gatattgaat gccaacggtt ggatgcaata cccgaccggt gcgcattttg atatcgacac
    39181 cctgcgtatg gagatgacca gcttcagcga gctggtcttt aacccggtca gccaggtgaa
    39241 atttgtgcac accgtcatgg cgggctacgt gaccggggcc atgtttatta tggcgatcag
    39301 cgcctggtat ttactgcgcg gacgggagcg cgatgtcgca ttacgctcgt ttgccatcgg
    39361 ttccgtcttc ggtactctgg cgattatcgg taccctgcaa ctcggagaca gttctgcgta
    39421 tgaagtcgcg caagtacaac cggtaaaact ggcggcgatg gaaggggagt ggcaaacgga
    39481 acctgctcct gcaccgttcc atgtggttgc ctggccggaa caggatcaag agcgtaacgc
    39541 ctttgccatc aaaattcccg cgctgctagg gatcctcgcc actcactcat tagataaacc
    39601 cgtgccgggt ctgaagaatt tgatggctga aacctaccca cgcttgcaac gcggacgtat
    39661 ggcctggctg ttaatgcagg aaatatctca aggcaatcgt gagccgcatg tgttgcaggc
    39721 attccgggag ctggaaggcg accttggtta cggcatgttg ctctcccgct atgcgccgga
    39781 tatgaatcat gtcacagccg cacagtacca ggcggcgatg cgtggcgcga tacctcaggt
    39841 tgcgccggta ttctggagtt tccgcatcat ggtgggctgt ggttccctgc tgctactggt
    39901 gatgctgatt gcgcttgtcc agacgctgcg tggcaaaatc gaccagcatc gctgggtgct
    39961 gaaaatggcg ctctggagtt tgccgttgcc gtggattgcg attgaagccg ggtggtttat
    40021 gaccgagttt ggtcgtcagc cgtgggcgat acaggacatc ttaccgacat actccgcgca
    40081 ctccgcttta accacaggac aactggcttt ctcactgatc atgatcgtag gtctttacac
    40141 cctgttctta atcgccgaag tctacctgat gcagaaatat gcccgtctgg ggccgagcgc
    40201 gatgcagagt gaacaaccga cgcagcaaca ggggtaaagg agaaaatcat gtttgattat
    40261 gaaacattgc gcttcatctg gtggctgctg attggcgtga tcctggtggt ctttatgatc
    40321 tccgacggat ttgacatggg gatcggctgt ttgctgccgc tggtggcgcg taatgatgat
    40381 gaacgccgga tagtgattaa tagcgttggt gcgcactggg aaggcaatca ggtctggttg
    40441 atcctcgcag gtggggcatt atttgccgcc tggcccagag tgtatgcagc ggcgttttcc
    40501 ggcttttatg tggcgatgat cctggtgctg tgctcgctgt tcttccgtcc gctggccttt
    40561 gattatcgcg gaaaaattgc cgatgcacgc tggcgtaaaa tgtgggatgc cggtctggtc
    40621 atcggcagtc tggtgccgcc ggtagtcttc ggtatcgcct tcggcaactt gttgctcggc
    40681 gtgccgtttg ccttcacacc gcaattacgc gtggagtatc tcggcagctt ctggcaactg
    40741 ctgacgccat tccctttatt gtgtggtttg ctcagccttg ggatggtgat tttgcaaggt
    40801 ggcgtctggt tacaactgaa aactgttggt gtgattcatc tgcgttcaca gctggcgact
    40861 aaacgcgctg cactgttggt gatgctgtgc tttttgctgg caggttactg gctgtgggtc
    40921 ggtattgatg gctttgtact gctcgcccag gatgctaacg gtccttccaa tccgttaatg
    40981 aaactggtgg cagtgctacc tggtgcctgg atgaataatt tcgtcgaatc gcccgttttg
    41041 tggatcttcc cgctgctggg gttcttctgc ccattgctga cggtgatggc gatttatcgt
    41101 ggtcgcccgg gttggggatt tttgatggca tcattgatgc aatttggcgt gattttcacg
    41161 gcaggcatca cgctgttccc ctttgtcatg ccgtcaagtg tgagtccaat ctccagcctg
    41221 acgttgtggg acagcacttc cagtcagctg acgctgagca ttatgttggt aatcgtgctg
    41281 atatttttgc ccattgtgtt gctctacact ctctggagct actacaaaat gtggggacgc
    41341 atgacaacag aaactctccg ccgtaacgaa aacgagttgt actaaggagc aggaacatgt
    41401 ggtatttact ttggttcgtc ggcattttgt tgatgtgttc gctctccacc cttgtgttgg
    41461 tatggctgga cctgcgtctg aaaagttaac gaacgtaggc ctgatgcggc gcattagcat
    41521 cgcatcaggc aatcaataat gttagatatg aaaagcggaa acatatcgat gaaagcgatc
    41581 ttaatcccat ttttatctct tctgattccg ttaaccccgc aatctgcatt cgctcagagt
    41641 gagccggagc tgaagctgga aagtatggtg attgtcagtc gtcatggtgt gcgtgctcca
    41701 accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac ttggccggta
    41761 aaactgggtt ggctgacacc gcgcggtggt gagctaatcg cctatctcgg acattaccaa
    41821 cgccagcgtc tggtagccga cggattgctg gcgaaaaagg gctgcccgca gtctgctcag
    41881 gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc cttcgccgcc
    41941 gggctggcac ctgactgtgc aataaccgta catacccagg cagatacgtc cagtcccgat
    42001 ccgttattta atcctctaaa aactggcgtt tgccaactgg ataacgcgaa cgtgactgac
    42061 gcgatccttt gcagggcagg agggtcaatt gctgacttta ccgggcatcg gcaaacggcg
    42121 tttcgcgaac tggaacgggt gcttaatttc ccgcaatcaa acttgtgcct taaccgtgag
    42181 aaacaggacg aaagctgttc attaacgcag gcattaccat cggaactcaa ggtgagcgcc
    42241 gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga gatatttctc
    42301 ctgcaacagg cacaaggaat gccggagccg gggtggggaa ggatcaccga ttcacaccag
    42361 tggaacacct tgctaagttt gcataacgcg caattttatt tactacaacg cacgccggag
    42421 gttgcccgca gtcgcgccac cccgttattg gatttgatca tggcagcgtt gacgccccat
    42481 ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtgctgtt tattgccgga
    42541 cacgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac gcttccaggt
    42601 cagccggata acacgccgcc aggtggtgaa ctggtgtttg aacgctggcg tcggctaagc
    42661 gataacagcc agtggattca ggtttcgctg gtcttccaga ctttacagca gatgcgtgat
    42721 aaaacgccgc tgtcattaaa tacgccgccc ggagaggtga aactgaccct ggcaggatgt
    42781 gaagagcgaa atgcgcaggg catgtgttcg ttggcaggtt ttacgcaaat cgtgaatgaa
    42841 gcacgcatac cggcgtgcag tttgtaatgc ataaaaaaga gcattcagtt acctgaatgc
    42901 tctgaggctg atgacaaacg aagaactgtc taatgcgtag accggaaaag gcgttcacgc
    42961 cgcatccggt cactttcagt tttactcttt ctcggagtaa ctataaccgt aatagttata
    43021 gccgtaactg taagcggtgc tggcgcgttt aatcacacca ttgaggattg cgcctttaat
    43081 attaacgcca gcctgttcca gacgctgcat tgacaagctc acctctttgg cggtgttcaa
    43141 gccaaaacgc gcaaccagca ggctggtgcc gacagagcgt cccacgaccg cagcatcgct
//
LOCUS       DAJTRQ010000017        42790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000017 DAJTRQ010000000
VERSION     DAJTRQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..516)
                     /locus_tag="OO332_001081"
     CDS             complement(<1..516)
                     /locus_tag="OO332_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5941643.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFG"
     gene            complement(656..1843)
                     /gene="metC"
                     /locus_tag="OO332_001082"
     CDS             complement(656..1843)
                     /gene="metC"
                     /locus_tag="OO332_001082"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5941644.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2095..2829
                     /gene="exbB"
                     /locus_tag="OO332_001083"
     CDS             2095..2829
                     /gene="exbB"
                     /locus_tag="OO332_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5941645.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            2836..3261
                     /gene="exbD"
                     /locus_tag="OO332_001084"
     CDS             2836..3261
                     /gene="exbD"
                     /locus_tag="OO332_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5941646.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3533..4418)
                     /gene="yghA"
                     /locus_tag="OO332_001085"
                     /pseudo
     CDS             complement(3533..4418)
                     /gene="yghA"
                     /locus_tag="OO332_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            4609..5103
                     /locus_tag="OO332_001086"
     CDS             4609..5103
                     /locus_tag="OO332_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5941647.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(5143..6183)
                     /gene="gpr"
                     /locus_tag="OO332_001087"
     CDS             complement(5143..6183)
                     /gene="gpr"
                     /locus_tag="OO332_001087"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5941648.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            6340..7227
                     /locus_tag="OO332_001088"
     CDS             6340..7227
                     /locus_tag="OO332_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5941649.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7346..7633
                     /locus_tag="OO332_001089"
     CDS             7346..7633
                     /locus_tag="OO332_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5941650.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            7822..8940
                     /gene="hybO"
                     /locus_tag="OO332_001090"
     CDS             7822..8940
                     /gene="hybO"
                     /locus_tag="OO332_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5941651.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            8943..9929
                     /gene="hybA"
                     /locus_tag="OO332_001091"
     CDS             8943..9929
                     /gene="hybA"
                     /locus_tag="OO332_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5941652.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            9919..11097
                     /gene="hybB"
                     /locus_tag="OO332_001092"
     CDS             9919..11097
                     /gene="hybB"
                     /locus_tag="OO332_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5941653.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            11094..12797
                     /gene="hybC"
                     /locus_tag="OO332_001093"
     CDS             11094..12797
                     /gene="hybC"
                     /locus_tag="OO332_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5941654.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            12797..13291
                     /locus_tag="OO332_001094"
     CDS             12797..13291
                     /locus_tag="OO332_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5941655.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            13284..13772
                     /gene="hybE"
                     /locus_tag="OO332_001095"
     CDS             13284..13772
                     /gene="hybE"
                     /locus_tag="OO332_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5941656.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            13765..14106
                     /gene="hypA"
                     /locus_tag="OO332_001096"
     CDS             13765..14106
                     /gene="hypA"
                     /locus_tag="OO332_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5941657.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            14240..14488
                     /gene="hybG"
                     /locus_tag="OO332_001097"
     CDS             14240..14488
                     /gene="hybG"
                     /locus_tag="OO332_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5941658.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(14611..15477)
                     /gene="yghU"
                     /locus_tag="OO332_001098"
     CDS             complement(14611..15477)
                     /gene="yghU"
                     /locus_tag="OO332_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5941659.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            15682..17541
                     /gene="gss"
                     /locus_tag="OO332_001099"
     CDS             15682..17541
                     /gene="gss"
                     /locus_tag="OO332_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5941660.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            17833..19332
                     /gene="pitB"
                     /locus_tag="OO332_001100"
     CDS             17833..19332
                     /gene="pitB"
                     /locus_tag="OO332_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5941661.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(19381..20073)
                     /locus_tag="OO332_001101"
     CDS             complement(19381..20073)
                     /locus_tag="OO332_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5941662.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            20247..20960
                     /locus_tag="OO332_001102"
     CDS             20247..20960
                     /locus_tag="OO332_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5941663.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            20992..21750
                     /locus_tag="OO332_001103"
     CDS             20992..21750
                     /locus_tag="OO332_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941664.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            21796..23145
                     /locus_tag="OO332_001104"
     CDS             21796..23145
                     /locus_tag="OO332_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5941665.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            23145..23969
                     /locus_tag="OO332_001105"
     CDS             23145..23969
                     /locus_tag="OO332_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941666.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            23981..24541
                     /locus_tag="OO332_001106"
     CDS             23981..24541
                     /locus_tag="OO332_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5941667.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            24572..25642
                     /gene="lptF"
                     /locus_tag="OO332_001107"
     CDS             24572..25642
                     /gene="lptF"
                     /locus_tag="OO332_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5941668.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            25639..26718
                     /gene="lptG"
                     /locus_tag="OO332_001108"
     CDS             25639..26718
                     /gene="lptG"
                     /locus_tag="OO332_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5941669.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(26754..27926)
                     /locus_tag="OO332_001109"
     CDS             complement(26754..27926)
                     /locus_tag="OO332_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5941670.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(27926..28174)
                     /locus_tag="OO332_001110"
     CDS             complement(27926..28174)
                     /locus_tag="OO332_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5941671.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            complement(28207..29121)
                     /locus_tag="OO332_001111"
     CDS             complement(28207..29121)
                     /locus_tag="OO332_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5941672.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(29118..30806)
                     /locus_tag="OO332_001112"
                     /pseudo
     CDS             complement(29118..30806)
                     /locus_tag="OO332_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            31210..32352
                     /locus_tag="OO332_001113"
                     /pseudo
     CDS             31210..32352
                     /locus_tag="OO332_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(32476..33246)
                     /gene="glcC"
                     /locus_tag="OO332_001114"
     CDS             complement(32476..33246)
                     /gene="glcC"
                     /locus_tag="OO332_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5941673.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            33492..34991
                     /gene="glcD"
                     /locus_tag="OO332_001115"
     CDS             33492..34991
                     /gene="glcD"
                     /locus_tag="OO332_001115"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCR5941674.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            34991..36043
                     /gene="glcE"
                     /locus_tag="OO332_001116"
     CDS             34991..36043
                     /gene="glcE"
                     /locus_tag="OO332_001116"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5941675.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            36054..37277
                     /gene="glcF"
                     /locus_tag="OO332_001117"
     CDS             36054..37277
                     /gene="glcF"
                     /locus_tag="OO332_001117"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCR5941676.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            37282..37686
                     /locus_tag="OO332_001118"
     CDS             37282..37686
                     /locus_tag="OO332_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCR5941677.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            37708..39879
                     /gene="glcB"
                     /locus_tag="OO332_001119"
     CDS             37708..39879
                     /gene="glcB"
                     /locus_tag="OO332_001119"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5941678.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            40234..41916
                     /gene="glcA"
                     /locus_tag="OO332_001120"
     CDS             40234..41916
                     /gene="glcA"
                     /locus_tag="OO332_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCR5941679.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
BASE COUNT        10202 a        10563 c        11428 g        10597 t
ORIGIN      
        1 gccaaaactg gttaccacgc tgacccacag caatccactt aaccagttga aaaactggaa
       61 gcggcggttt ggcagaccgg aaattcccgc catggttggc agcagcgtac ggacaaatgc
      121 aagaaaacgt ccagccagca gcgccagcag accgtggcgg tcaaacatgc aggtggcgcg
      181 ctggtgatat ttagcaggaa gctgtgccag ccagcctttc accgttttgg tattccctaa
      241 ccagcgcccc tgaatataac ttagccagca gcccagactt gctgcggcgg tcagaatcgc
      301 aatcgtaggc agaaaatcca taaccccttg ggcaatcaat gcgcctgcca gtatcaacaa
      361 gctgtcgcct ggcaaaaatg aggcgggcag caggccgttt tctaaaaaca gcgtggcaaa
      421 catgacaaag taaacaacgc taacaatatg aggatccgcc agcgcggcaa agtcgtgttg
      481 ccagagcgca gcgatgatat cttgaataac agccatggac tttcctgtgg aacagcgttt
      541 aagcttttat ctgcctattg tactcctgaa tcgtccggga cgccttgatc ccggacgcaa
      601 caatttaaga cttttcacaa taaaatgtct gcaaaattgt ccaaaagtgg caatgttata
      661 caatccgtgc aaaaccggcg tccagatcgg caatcagatc gtcgacatct tccagaccaa
      721 tatgcaggcg aatcaaggtc ccgctaaaat cgatctcgcc ttgtgggcga atggcggcga
      781 tatgttctgg ttgatttgcc aggatcaacg attcataccc gccccacgag taggccatgc
      841 tgaataaact gaagttatcc agatagttcg ccagctcttc atcattgagt tttttcttaa
      901 gcacaaagga aaatagcccg ctgctgcctg taaagtctcg tttccagaat tcgtgtcctt
      961 tactgccagg cagggcaggg tggttaactc gcgcaacttg cggatgttct gccagccatt
     1021 cggccacttt cagactgctt tcatgatgtt gacgcaaacg cacacctaat gtgcgcaggc
     1081 cacggctggt tatataggcg gtatcggcat cgaccatctg gcccatcaga taggcatttt
     1141 cccgtagctg ctcccagcaa cgggcattgc acacggcagt gccaatcatc gcatctgaat
     1201 gcccaaccag atatttggtg gcggcctgaa tagaaacatc gatgccaaaa tccagcgcct
     1261 taaacagcac accggctgcc caggtgttgt cgatcataat gatggcatcc ggcaccacac
     1321 tgcgcacggc ggcaacaatc gccggaacgt cgtggacttc catagtgatg gaacccggcg
     1381 attccagaaa cacgatttta gtgtttggct gcagatgctt aacgatatcg gcaccaatca
     1441 gcggatcaaa ccacgatgtc gttacgccca gtttgctgag gattttgcta cagaaatcct
     1501 gactcggttc ataggcggtg ttggtcatca acacatgatc gccctgttcg acaaaagcaa
     1561 gaatcgaatt agcaaccgct gccgccccgc agggaaatag cgcgcagcct gcgccacctt
     1621 ccagttcaca catcgcttgt tgtaaggaaa aatgggttaa cgttccgcgc cgtccataga
     1681 acaactcgcc attggcgcga ttgcgcgtcg cgtgcttttt ggcttccata ctctcaaata
     1741 ccagcgaaga agcgcgctga atcacgctat ttaccgcgcc aagagtgtat tttttgctgc
     1801 gtcctgcatt caccagttga gtatcaagct ttttgtccgc catgtcggga ttcctgtttt
     1861 tatacgtctg gatgtctaaa ctagcatgaa tattcgcggg cgcatcctga agaattggca
     1921 taaagcagaa aattttcgca aatggcgtgc tggtgacttt tttactgcgt aaacgcaagg
     1981 aaaagaaagc aaaggtacgg cagtatgcaa atagtaatga gaacgactat caattcgacg
     2041 tcgttttgat attattatgc gcagattttg tgacttgcgt cctggagata cacagtgggt
     2101 aataatttaa tgcagacgga cctttccgtc tggggtatgt atcagcacgc cgatattgtc
     2161 gttaagtgcg tgatgattgg gcttattttg gcctccgtag tcacctgggc aatcttcttc
     2221 agtaagagcg tagagttctt caatcagaag cgtcgcctta agcgcgagca gcaactgctg
     2281 gctgaagcac gttccttaaa ccaggccaac gatatcgccg ctgattttgg tagcaaaagc
     2341 ttaagcctgc atttgctcaa tgaagcgcag aatgagctgg aactgtcaga aggcagcgac
     2401 gataacgaag gtattaaaga acgtaccagc ttccgtctgg agcgtcgggt cgccgcagtg
     2461 ggtcgtcaaa tgggccgcgg taacggctat ctggcaacca tcggcgcgat ttctccgttc
     2521 gttggtctgt ttggtacggt ctggggcatc atgaacagct ttattggtat cgcgcaaacg
     2581 cagaccacta acctggcagt cgttgcgccg ggtatcgcag aagctctgtt agcaacggca
     2641 atcggcctcg tggcagcgat tcctgcggtc gttatctata acgtatttgc acgccagatt
     2701 ggcggcttta aagcgatgct gggtgatgtt gcagcgcagg tattgttgct gcaaagccgt
     2761 gacctggatc tggaagccag cgccgctgcg catccggttc gtgtcgcaca aaaattacgc
     2821 gcaggataat gtccgatggc aatgcatctt aacgaaaacc tcgacgataa cggcgaaatg
     2881 catgatatca acgtgacgcc gtttatcgac gtgatgttgg ttctgctgat tatctttatg
     2941 gtggcggcac cgttagcgac ggtagatgtg aaggtgaact tgcctgcttc taccagcacg
     3001 ccgcagccgc gcccggaaaa accggtttat ctgtcggtga aggcagacaa ctcgatgttt
     3061 atcggtaacg atccggtcac cgatgaaaca atgattacgg cgttgaatgc gttaaccgaa
     3121 ggcaagaaag acaccaccat cttcttccga gcggacaaaa ccgtcgatta cgagacgttg
     3181 atgaaggtaa tggatacgct gcatcaggcg ggttacctga agataggtct ggtcggcgaa
     3241 gaaaccgcca aagcgaagta aagtagaatt gcctgatacg ctacgctcat caggcctaca
     3301 aaatctattg caacatgttg aatcttcgtg cgtttgtagg ccggataagg cgttcacgac
     3361 gcatccggca ttaggtgctc aatgcctgat gcgctacgct tatcaggcct acaaaatcta
     3421 ttgcaacatg ttgaatcttc atgcgtttgt aggcggataa ggcgttttcg ccacatcagg
     3481 taagagtgaa ttcacaatga tgcccggttg cttttcacaa ccgggcattt ttttaaccta
     3541 aatgctcgcc gccgcacacg ccgtgcactt ctgcggtgac gtagctcgac tcctgacttg
     3601 ccagataaac atatacaggg gccagttccg ccggttgccc cgcacgcttc atcggcgttt
     3661 tctgaccaaa ctgcgggatc ttatcctgcg tttgtccgcc ggaaatttgc agtgccgtcc
     3721 agatagggcc tggcgcgaca atattcaccc gaataccttt ctccgcgacc tgttttgcca
     3781 ggccacggct gtagttcaga atcgccgcct tcgtagccgc atagtccagt aaatgcggac
     3841 ttggctggta tgcctggatt gacgaagtgg tgatgatact tgcacctttc ggtagcaggg
     3901 ggatcgcttc ctgggttagc cagaacagcg cgaaaacgtt aatggcaaag gtcttttgaa
     3961 actgttcgct ggtgaggtct gcaatatccg gaatggcaac ctgtttcccg gcgaccagcg
     4021 ccataatatc cagcccgcct aacgccttgt gcgcttcgtg aaccagcgaa cgggcgaatt
     4081 tctcatcgct taaatcgcct ggcagcagaa cggctttgcg tccgcattct tcaatgatct
     4141 ttttcacatc ctgagcgtct tcttcttcca cgggaagata actgatcgcc acgtcagccc
     4201 cttctcgcgc gtaagcgatg gcggcagcgc gacctattcc ggaatcgccc cctgtcacca
     4261 gtgctttacg atctttcagg cgagcgctac caacataggt tttctcgccg caatccggta
     4321 tcggtgtcat cttcgcctgg atgcctggcg tcggttgttt ctgtttggga tattcaccag
     4381 tgtaatactg cgtggtcggg tcattttaaa tgagacatcg tttttctccc ttcaggttca
     4441 acgtccttta agggtagacg ctctcgatgc gttgataagg gaaccaggaa gatccctaaa
     4501 cctcagaatt atgcgacaaa ggtttaacgt atatgttgat ttgctgttgc gtgctgttta
     4561 ctcaattgcg atatactgtt gcccgtttta actacacgac aggaatgtat ggaacgtttt
     4621 tttgaaaatg caatgtatgc ttctcgctgg ctgcttgccc ccgtgtactt tggcctttcg
     4681 ctggcgttag ttgccctggc gctgaagttc ttccaggaga ttattcacgt actgccgaat
     4741 atcttctcga tggcggaatc agatttgatc ctcgtgttgc tgtcgctggt ggatatgacg
     4801 ctggttggcg gtttgctggt gatggtgatg ttttccggtt atgagaattt cgtctcacag
     4861 ctggatatct ccgagaacaa agagaagctg aactggctgg ggaaaatgga cgcaacgtcg
     4921 ctgaaaaaca aagtagcagc gtcgattgtg gcaatttctt ccattcactt actgcgcgtc
     4981 tttatggatg cgaaaaatgt cccggataac aaactgatgt ggtacgtcat tatccatctg
     5041 acgtttgtgc tttctgcatt tgtgatgggc tatcttgacc gactgactcg tcataatcac
     5101 tgatctgatt cgggcgcggt tcgcgcgccc gttattaaca ggtcatttat cggacgacgc
     5161 ctgccacaga ttcagttcgc catcggcgat atgctgatca atctgcgcca gctcctcggt
     5221 gctaaatgtc agattattca gcgcctgcac gttctcctct agttgctccg cgcggctggc
     5281 accaatcaat accgacgtca cgcgatcatc tttcagcaac cagcttaacg ccatttgcgc
     5341 cattgattgt ccacgctgct gtgccatttc attcaataag cgcaggctgt tgaggttggc
     5401 ttcggtaagc attttcggcg tcagaccacg aattttattc ccttcacgat gcatccgtga
     5461 atcttgcgga atgccgttga gatattttcc ggtcagcaat ccctgagcca gaggagtaaa
     5521 ggcaatacag cccacgccgt tattttgcag ggtatccagc aggccgcttt tatccaccca
     5581 gcggttcagt aaattgtacg aaggttgatg aattaaaagc ggaattttcc actcgtgcag
     5641 caactcgacc attttttgcg tccgctctgg cgagtaagag gagatcccga catacagcgc
     5701 cttaccgctt tgtaccgcat gagccagcgc agaggcggtt tcttccatcg gcgtattttc
     5761 atcgacgcga tgagagtaaa agatatcgac atactcaagc cccatacgct tcaggctttg
     5821 gtcgaggctg gcgagcaggt atttacgtga accgccagag ccgtacgggc cgggccacat
     5881 atcgtagcca gccttggtag agataatcag ttcatcgcga taagcagcaa aatcctcgcg
     5941 cagcaggcga ccaaagttct cttctgcgct tcctggaggc ggcccgtaat tgttggctaa
     6001 atcaaagtgc gtaatgccta aatcaaacgc tttacgcagg attgcacgct gtgattccag
     6061 cgcgttaacg tgaccgaaat tgtgccataa accgagcgat aacgcgggca ggcgtaaacc
     6121 actttttccg caatagcggt actgcatctg cccgtaacgt tcgggattcg ctaaccagac
     6181 catgacctct cctttccacc gttcaatttc gaaacaatgt ttctagttta gcgattcgcc
     6241 agcgcgtatc ccgtagtctg gctcacagag tgacgaaaaa ctgggcaaaa acacgcgctt
     6301 atgctttgct taaaaaaaca ccagttgagg aatgcaacga tgccgcgttt aaccgccaaa
     6361 gatttcccac aagagttgtt ggattactac gactattacg ttcacgggaa aatttcgaaa
     6421 cgtgagttcc tcaaccttgc ggcgaagtat gcggtgggcg ggatgacggc attagcgttg
     6481 tttgatttgc tcaagccaaa ttatgcgctg gcgactcagg tagagtttac cgacccggag
     6541 attgttgctg agtacatcac gtatccttcg ccaaatggtc acggcgaggt acggggttat
     6601 ctggtgaaac ccgcaaaaat gagcggcaaa acgccagccg tgggggtggt gcatgagaat
     6661 cgtggactga atccgtatat cgaagatgtg gcacggcgag tggcgaaggc ggggtatatc
     6721 gccctggcac ctgacggatt aagttccgtt ggaggttatc cgggaaatga tgataaaggt
     6781 cgtgagctgc aacagcaggt tgatccaacc aaactgatga atgatttctt tgccgcaatt
     6841 gagtttatgc aacgctatcc gcaagcgaca ggtaaagtgg gtattaccgg attttgctat
     6901 ggcggtggcg tatcgaacgc ggcggctgtc gcgtatccgg aactggcctg cgcggtgccg
     6961 ttttatggtc gtcaggcacc cactgccgat gtggcgaaga ttgaagcgcc tttactactc
     7021 cacttcgcgg aactggacac ccgaatcaac gagggctggc ctccttacga ggcggcgttg
     7081 aaagacaata ataaggttta tgaggcttat atctatccgg gggttaatca cggattccat
     7141 aatgattcca cgccccgtta tgacaaatct gccgccgatc ttgcctggca aaggacactg
     7201 aaatggttcg ataaatatct ctcctgatag gtttatctct tacgggatta cgtcttaaac
     7261 aagtatgaaa aaatagcgtg cgcaaaagtc gttctttgcc taaaatatcg ctatatatgg
     7321 cgatatataa cgaatggggt gaacgatgaa taaccatttt ggtaaaggct taatggcggg
     7381 attaaaagca acgcatgccg acagtgcggt taatgtgaca aaattctgtg ccgattataa
     7441 acgcggtttt gtattaggct actcacaccg gatgtacgaa aagaccggag atcgccagct
     7501 tagcgcctgg gaagcgggta ttctgacgcg ccgctatgga ctggataaag agatggtaat
     7561 ggatttcttt cgtgagaata attcctgttc tacgttgcgc ttttttatgg ccggttatcg
     7621 cctcgaaaat tgatcaaaca tacgtattat cttgctttaa ttaattacac taatgcttct
     7681 tcccttcgtt ttagcgcccc gccgcagtat catgatatcg ataaccataa taaatgtgtg
     7741 gtaaatggcg catcgatcgc attattgatt ttgcgattga ggcaaaatat atgccaggtc
     7801 ctcgcaacgg aataactata aatgactgga gataacaccc tcatccattc tcacggcatt
     7861 aaccgtcgtg atttcatgaa gctttgtgca gcattagccg ccaccatggg gttaagtagc
     7921 aaagccgctg cagagatggc cgaatcggtt actaacccgc agcgtccgcc agttatctgg
     7981 attggcgcgc aggagtgcac cggttgtacg gaatctctgc ttcgtgcaac gcatccaacg
     8041 gtagaaaacc tcgtgctgga gactatctct ctggaatatc acgaagtgct ttccgccgcc
     8101 ttcggtcatc aggtcgaaga gaacaaacat aacgctctcg agaagtacaa agggcagtat
     8161 gtgttagtgg tggatggttc catcccatta aaagataacg gtatttattg catggttgcc
     8221 ggtgagccga ttgtggatca catccgcaaa gcggcggaag gcgcagcagc cattatcgct
     8281 attggttcct gctccgcgtg gggcggtgtt gccgcagctg gagttaaccc aactggcgca
     8341 gtcagcttgc aagaagttct gccaggcaaa accgttatca atattccggg ctgcccgccg
     8401 aacccgcaca acttcctcgc gaccgttgcg cacatcatca cttacggcaa accgccgaaa
     8461 ctggatgaca aaaaccgtcc gaccttcgcc tatggccgtc tgattcacga acactgcgaa
     8521 cgtcgcccgc acttcgatgc tggtcgtttt gccaaagagt tcggtgatga aggccaccgc
     8581 gaaggctggt gcctgtacca cctcggctgt aaagggccag aaacttacgg caactgctca
     8641 acgctgcaat tctgcgatgt tggcggtgtg tggccggtgg cgattggtca cccgtgctat
     8701 ggctgtaacg aaaaaggtat cggcttccat aaaggcatcc atcagcttgc caacgtcgaa
     8761 aatcagactc cgcgttcaca gaaaccggat gttaacgcta aagagggcgg caacgtctct
     8821 gcaggcgcta ttggtttgct cggcggtgtg gttgggctgg ttgccggtgt cagcgtgatg
     8881 gcggtgcgtg aactgggtcg tcagcaaaag aaagataacg ctgactcacg gggagaataa
     8941 ccgtgaacag acgtaatttt attaaagcag cctcctgcgg ggcattgctg acgggcgcgc
     9001 tgccgtctgt cagtcatgcg gctgctgaaa accgcccgcc aattccggga tcgctgggga
     9061 tgttgtacga ctcgaccttg tgcgtaggct gccaggcttg cgtcaccaag tgtcaggata
     9121 tcaacttccc tgaacgtaac ccgcaagggg aacagacctg gtcgaacaac gacaaactgt
     9181 cgccgtatac caataacatc attcaggtgt ggaccagcgg cacaggggtc aacaaagacc
     9241 aggaggagaa cggctacgcg tacattaaga aacagtgtat gcactgcgtc gatccgaact
     9301 gtgtctctgt gtgcccggtc tctgcactga aaaaagatcc gaaaaccggc attgtccatt
     9361 acgacaaaga cgtgtgcacc ggttgccgtt actgcatggt cgcctgtccg tacaacgtgc
     9421 cgaagtacga ctacaacaac ccgtttggtg cgctgcataa gtgcgagctg tgcaaccaga
     9481 aaggtgtgga acgtctcgat aaaggcggtc tgcctggctg cgtagaagtg tgcccggcgg
     9541 gcgcggtgat tttcggtacg cgtgaagagc tgatggcgga ggcgaaaaaa cgtctggcgc
     9601 tgaagcctgg cagcgaatac cactatccgc gtcagacgct gaaatctggc gacacttacc
     9661 tgcatacggt gccgaaatat tatccgcatc tgtacggcga gaaagagggc ggcggtactc
     9721 aggttctggt actgacgggt gtgccttatg aaaatctcga cctgccgaaa ctggacgatc
     9781 tttctaccgg tgcgcgttcc gaaaatattc aacacaccct gtataaaggc atgatgctac
     9841 cactggctgt gctggcgggc ttaaccgtgc tggttcgtcg caacaccaaa aacgaccatc
     9901 acgacggagg agacgatcat gagtcatgat ccacaaccgc tgggtggcaa aatcatcagt
     9961 aaaccggtca tgatttttgg accgttaatc gtcatctgta tgctcctgat tgtgaagcgt
    10021 ctggtgttcg gtctgggctc tgtctctgac ctgaacggcg gcttcccgtg gggcgtgtgg
    10081 atcgcgtttg acctgctgat tggcaccggc tttgcctgtg gcggctgggc gctggcgtgg
    10141 gcggtatacg tctttaaccg tgggcaatac catccgctgg tgcgtccggc gctgttggcg
    10201 agtctgtttg gttactcact gggtggcttg tcgatcacta tcgacgtggg tcgctactgg
    10261 aacctgccgt acttctacat tccgggtcac ttcaacgtga actcggtact gttcgagacg
    10321 gcggtctgta tgaccatcta catcggcgtg atggctctgg agtttgctcc ggcactgttt
    10381 gaacgtctgg gctggaaggt gtcgctacaa cgtctgaaca aggtgatgtt cttcatcatc
    10441 gcgctcggtg cgctgctgcc gaccatgcac cagtcttcaa tggggtcgct gatgatctcg
    10501 gcgggctaca aggtgcatcc gctctggcag agctatgaaa tgttgccgct gttctcgctg
    10561 ctgacggcgt tcatcatggg cttctcgatt gtcatctttg aaggttcact ggtgcaggcg
    10621 ggtctgcgtg gcaacggtcc ggatgaaaag agcctgttcg tcaagctgac caacaccatc
    10681 agtgtgttgc tggcgatttt catcgggctg cgctttggcg agcttatcta tcgcgacaag
    10741 ctgtcgttag cgtttgccgg tgacttctac tccgtgatgt tctggattga agtcctgctg
    10801 atgctcttcc cgctggtcgt tctgcgtgtg gcgaagctgc gtaatgattc ccgcatgctg
    10861 ttcctgtcag cactgagcgc actgttaggt tgtgcaacct ggcgtctgac ctattcgttg
    10921 gtggcattca acccgggcgg cggttacgcc tacttcccga cctgggaaga actgttgatt
    10981 tctattggtt ttgtggctat tgagatttgc gcttacatcg tactcattcg tctactgccg
    11041 atacttcctc ctttaaaaca aaacgatcat aatcgtcatg aggcgagcaa agcatgagcc
    11101 agagaattac tattgatccg gtaacccgta ttgaggggca tttacgcatc gattgcgaaa
    11161 tcgaaaatgg cgtcgtttcg aaagcatggg cttccggtac catgtggcgc ggcatggaag
    11221 agatcgtgaa aaaccgcgat ccgcgcgatg catggatgat tgtgcaacgt atctgtggcg
    11281 tatgtactac cactcacgcg ctgtcttccg ttcgtgcggc agaaagtgcg ctgaatatcg
    11341 acgttccggt taacgcgcaa tacatccgta acatcattct ggctgcgcac accacgcatg
    11401 accatatcgt tcatttctat cagctttcgg cgctggactg ggtggacatc acttctgcac
    11461 tgcaagctga cccaaccaaa gcctccgaaa tgctgaaagg cgtttcgacc tggcatctga
    11521 acagtccgga agagttcact aaagttcaga acaagatcaa agatctggtt gccagcggtc
    11581 agttgggtat tttcgccaat ggctactggg gtcacccggc gatgaaactg ccgccggaag
    11641 tgaacctgat tgcggtagcg cactacctgc aggcgctgga gtgccagcgt gacgctaacc
    11701 gcgtcgtggc gctgctgggc ggtaaaacgc cgcacattca gaacctggca gtgagtggtg
    11761 tcgcgaaccc aatcaacctc gacgggttgg gcgtgttgaa ccttgagcgc ctgatgtaca
    11821 tcaagtcttt catcgacaaa ctgagcgact ttgttgagca ggtttataag gttgataccg
    11881 cggttatcgc cgcgttctac ccggaatggc tgacgcgcgg taaaggtgcg gtgaactacc
    11941 tgagcgtgcc ggaattcccg accgacagca aaaacggcag cttcctgttc ccgggcggct
    12001 acattgagaa tgcggatctg tcctcgtatc gtccgatcac ttctcattcc gatgaatacc
    12061 tgatcaaagg cattcaggaa agcgcgaagc actcctggta taaagacgaa gcgccacagg
    12121 cgccgtggga aggtaccacc attccggctt atgatggttg gtctgacgac gggaaatatt
    12181 cctgggtgaa atcaccgact ttctacggca aaacggtaga agtgggtccg ctggctaata
    12241 tgctggtgaa actggcggca ggtcgcgaat ctacccagaa caaactgaat gaaatcgttg
    12301 cgatttatca gaaactgact ggcaacacgc tggaagtggc acagctgcac tccacgctgg
    12361 gccgtattat tggtcgtacc gttcactgct gtgaattgca ggatatcctg caaaaccaat
    12421 acagtgcact gatcaccaat atcggcaaag gcgatcacac cacctttgtg aagccgaaca
    12481 ttccggcaac gggtgagttc aaaggtgttg gcttcctcga agcgccgcgc ggtatgctct
    12541 ctcactggat ggttattaaa gacggtatca tcagcaacta ccaggcggtt gttccatcaa
    12601 cctggaactc tggtccgcgt aacttcaatg atgacgtcgg tccttacgag cagtcgctgg
    12661 tgggtacacc ggttgccgat ccgaataaac cgctggaagt ggtgcgtacc attcactcct
    12721 tcgacccgtg catggcctgt gcggtacacg tagtggatgc cgacggcaac gaagtggttt
    12781 cagtgaaggt tctgtaatgc gtattttagt cttaggggtc ggcaatattt tgctgaccga
    12841 tgaagccatc ggcgtgcgga ttgtcgaagc gttagagcaa cgatacattc tgccggatta
    12901 tgttgagatc ctcgatggcg gcacggcggg aatggagctg cttagcgaca tggcaaatcg
    12961 cgatcatctg attattgcgg atgccattgt gtcgaaaaag aacacgccgg gaacgatgat
    13021 gatcctgcgg gatgaagaag ttccggcgtt gtttaccaac aaaatctctc cgcatcagct
    13081 tggcctggcc gacgtattgt cggccctgcg cttcaccggt gagtttccga aaaagctgac
    13141 cctggtcggc gtgatcccgg aatcgctgga gccacacatc ggcttaacgc cgacggttga
    13201 agcaatgatt gaacctgcgc ttgagctggt tctggctgcg ctgcgcgaat caggcgtgga
    13261 agccatccca cgggaggcga ttcatgactg aagagatagc aggtttccag acctccccga
    13321 aggcgcaagt acaggcagcg tttgaagaaa ttgcccggcg ttcgatgcac gatctctctt
    13381 ttctgcatcc ttcaatgccg gtgtatgttt ctgattttac gctgttcgaa ggtcagtgga
    13441 cggggtgtgt gatcaccccg tggatgctga gtgcagttat cttccccggc ccggatcaac
    13501 tctggccgct gcgcaaagtg agtgaaaaaa ttggtctgca actgccgtat ggcaccatga
    13561 cctttaccgt tggcgaactg gacggtgttt cgcaatacct ctcctgttcg ctgatgtcgc
    13621 cgctttcgca cagcatgtcg attgaagaag gccaacgcct gacggatgac tgcgcacgaa
    13681 tgatcctttc gttgccagtc accaatccgg atgtaccaca cgcagggcgt cgcgccctgc
    13741 tgtttggccg caggaggggc gaaaatgcat gagttgtcgc tttgccagag cgccgttgaa
    13801 attatccaac ggcaggcgga gcagcacgat gttaagcgcg tcaccgccgt gtggctggaa
    13861 attggcgcgc tctcctgcgt tgaggagagc gccgtccgtt ttagttttga aattgtctgc
    13921 cacggaacgg tggcgcaagg gtgcgattta catatcgtct ataaacccgc ccaggcctgg
    13981 tgctgggatt gcagccaggt ggtggagatt catcagcacg atgcgcagtg tccgctctgt
    14041 cacggcgagc ggttgcgtgt cgataccggc gattcgctga tcgtcaaaag tattgaagtt
    14101 gaataagact gaggacaacg cctaaaaagc gcacggacgg gcccctcgcc cctccgggga
    14161 gagggttagg gtgagaggga acacccgcta aaggttcttt ttcgtgtttg tccggcaatc
    14221 gaaataaccg gagttaacaa tgtgtattgg cgttccaggc caggtgctgg ctgtcggtga
    14281 agatattcac cagcttgcgc aggttgaagt atgtggtatc aagcgcgacg tgaatatcgc
    14341 cctgatttgc gaaggtaacc ctgccgatct gctgggccag tgggtgctgg tacacgtcgg
    14401 atttgccatg agcatcatcg acgaagatga agccaaagcc acattagacg cactgcgcca
    14461 aatggattac gacattacca gcgcgtgatg attagctttt gtaggcctga taagacgcgg
    14521 cagcgtcgca tcaggcattg tgcacgattg ccggatgcgg cgtgaacgcc ttatccggcc
    14581 tggatggctt gctgcgacga acaccaaccc ttacccctga cgcttatctt ctgtatttgt
    14641 ctcaaaatca ctggcgtcat ggcgctcatg caactgctcg ttcagcggcc cgttggtgcg
    14701 gttaacaata cgcccacgtt tcactgccgg acgttcgccc acttctttcg cccagcgttg
    14761 cacatgttta taacttcccg catccagaaa ctctgcggca tcatacacgc cgccgagcac
    14821 cacgttgcca aaccacggcc aaatcgccat atccgcaatg gtgtactcat cgcccgcaac
    14881 aaacttatgc tgcgccagtt gcttatccag cacgtcgagc aggcgcttgg cttccatggt
    14941 aaagcggttg atggcgtact caattttcac cggcgcgtaa tggtaaaagt gaccaaaacc
    15001 accgccgagg aacggcgcgg caccctgtaa ccagaacagc cagttcaacg tttcagtccg
    15061 ttttgccaga tcctgcggca ggaagtagcc aaatttctcc gccagataaa gcaggatcga
    15121 accagattca aacacgcgga tcggcggatt atgcgtatga tcgcgcagcg ccgggatctt
    15181 cgagtttggg ttcacttcga caaagccgct ggagaattga tcgccatcgc caatacgaat
    15241 cagccaggcg tcgtactctg caccagtaac gcccagcgcc agtagctcct caagcataat
    15301 cgttactttc tgaccgttcg gcgttcccag cgaataaagt tgcaatggat gtttgccaac
    15361 gggcagcgtt ttttcatgcg tcggaccaga aaacgggcga ttgatattgg cgaacgcgcc
    15421 gccagcggat ttatcccacg tccagacttt cgcgggctga taagtattgt ctgtcatagt
    15481 gagttgcctt ctgagtggta gtgttgaagc agtgtagcag gtcacttgct gtacagaatg
    15541 tgaatttttt aaccctttgc gccagccgct gcgatgaggt gtatgattag tctgacctct
    15601 gtccggttaa tgtcgctgag caggtaatca gagcacaaca attacgccaa ctgcttaggt
    15661 tgtattgtta aaggtaaagt gatgagcaaa ggaacgacca gccaggatgc cccgttcggg
    15721 acattattgg gctacgcccc aggcggggta gcaatctact cttcagatta cagttctctc
    15781 gatccgcagg aatacgaaga tgacgccgta ttccgtagct atatcgacga cgaatatatg
    15841 ggccacaagt ggcaatgcgt tgaatttgct cgccgttttc tctttctgaa ttacggtgtg
    15901 gtctttactg acgtgggtat ggcgtgggag attttctcgc tgcgcttcct gcgagaagtg
    15961 gttaatgaca acatcctgcc attgcaggca tttcctaacg gctcgccgcg tgcgccggtc
    16021 gcgggtgcgc ttcttatctg ggataaaggc ggtgaattta aagacactgg ccatgtcgcc
    16081 atcattaccc aattgcatgg caacaaagtc cgtattgcgg aacagaacgt gattcattcc
    16141 ccgttgccgc aagggcaaca gtggacgcgc gagctggaga tggtggtcga aaacggctgc
    16201 tataccctta aagacacttt tgatgacacc accattctgg gctggatgat ccagacggaa
    16261 gatactgaat acagcttacc gcagccggaa attgcaggcg agctgctgaa aatcagtgga
    16321 gcgcgtctgg aaaacaaagg ccagtttgac ggtaaatggc tggatgaaaa agatccgcta
    16381 caaaacgcct atgtgcaggc caacggtcag gtgatcaatc aggatccgta tcattactac
    16441 accattaccg agagtgccga gcaggagcta attaaagcca ccaacgagct gcatctgatg
    16501 tatcttcacg caaccgacaa ggtgctgaaa gatgacaacc tgctggcgct gttcgacatt
    16561 ccgaaaatcc tctggccacg tttgcgtctc tcctggcagc gtcgccgtca ccatatgatc
    16621 actggtcgta tggatttctg catggatgag cgtggcctga aggtttacga atacaacgcc
    16681 gactccgcct cctgtcatac cgaagcgggc ttgatcctcg aacgttgggc ggagcagggc
    16741 tataaaggca acggcttcaa tccggcggaa gggctgatta acgaactggc tggtgcctgg
    16801 aaacacagtc gtgcacgtcc gtttgtccat atcatgcagg acaaagatat cgaggaaaac
    16861 tatcacgcgc agtttatgga gcaggcgctg caccaggcgg gctttgaaac gcgtatcttg
    16921 cgcgggctgg atgaactggg ctgggatgct gccgggcaac tgattgatgg ggaagggcga
    16981 ctggttaact gcgtgtggaa aacctgggcg tgggaaaccg cgtttgatca gattcgtgaa
    17041 gttagcgacc gtgagtttgc tgcggtgcca atccgtaccg gtcatccgca aaacgaagtg
    17101 cgtcttatcg acgtattgct gcgcccggaa gtgctggtct ttgagccgct gtggacggtg
    17161 atccccggca acaaagcgat tctgccgatc ctctggtcgc tgttcccgca ccatcgttac
    17221 ctgctggata ccgatttcac tgttaatgat gaattggtga aaacaggtta cgcagtgaaa
    17281 ccgatcgccg gtcgctgtgg tagcaatatc gacctggtcg gccatcatga agaggtgctg
    17341 gacaaaacca gcggtaaatt tgccgagcag aaaaacatct atcagcaact gtggtgtttg
    17401 ccgaaagtgg acggtaaata cattcaggta tgtaccttca ccgttggcgg caactacggt
    17461 gggacgtgtt tgcgcggtga tgaatcactg gtcatcaaaa aagagagtga tattgaaccg
    17521 ttaattgtgg tgaaaaagta atacatgttg tagcaacccc gtactgatta tgaagaataa
    17581 ttagtacggg gattaatcaa aatattcaat atttatatat tcaaatattt attcatttca
    17641 tatgtgaata tattaaccag tggaatacct gtgttttgat tatttctaaa ggttttgaat
    17701 gaatgcttat tgtctaatac acaagaaata acactctttt tatcgttaaa aaatgatatt
    17761 tcactttgcc catgccgtta aagtcactga taatgcgtcc gttcgtaaat tcaaaatggc
    17821 gtaatctaat atatgctaaa tttatttgtt ggccttgata tatatacggg gcttttgtta
    17881 ttacttgctc tggcatttgt gttgttctac gaagcaatca atggttttca tgacacggcg
    17941 aatgcggtgg caaccgttat ttatactcgt gccatgcagc cacaacttgc tgtggtgatg
    18001 gcggcatttt ttaacttttt tggcgtgtta ttgggcggac ttagcgttgc ctatgccatt
    18061 gtccatatgt taccaaccga tttgttgctg aatatggggt caactcacgg tctggcgatg
    18121 gtcttttcca tgctgctggc ggcgattatc tggaacctgg gaacgtggtt ctttggttta
    18181 ccggcctcca gttcgcacac cttgattggt gcgattatcg gcatcggttt aaccaacgcg
    18241 ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc gtgaagtaac caaaattttc
    18301 tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg cgggtggtct gattttcctg
    18361 ctgcgccgtt tctggagtgg gacgaaaaag cgtgaccgta ttcaccgcat tccggaagat
    18421 cgcaaaaaga aaaagggcaa acgtaaaccg ccattctgga cgcgtattgc gctgattgtt
    18481 tccgctgcag gcgtggcgtt ttcgcacggc gcgaacgacg ggcagaaagg gatcggcctg
    18541 gtaatgctgg tactggtggg gattgcccct gctggcttcg tcgtcaatat gaatgcgtcc
    18601 ggctatgaaa ttacccgtac ccgcgatgcc gttaccaact tcgaacacta cctgcaacag
    18661 catcctgaac tgccgcagaa gttgattgcc atggaacctc cattgcctgc agcatcgact
    18721 gatggcacgc aagtaacaga gtttcactgt catccggcaa atacctttga tgcgattgcg
    18781 cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg agccgttaag cgtgagtcag
    18841 cgcagccagc tgcgccgcat tatgctgtgc atctctgata cctccgcgaa gctggcgaaa
    18901 ctgccaggcg tcagtaaaga agaccagaac ctgctgaaga aactgcgcag cgatatgtta
    18961 agcaccattg agtacgctcc ggtgtggatc atcatggcgg tagcactggc gctcggcatt
    19021 ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg gtgagaagat tggtaagcgc
    19081 ggcatgacgt atgcgcaagg catggcggca caaatgacgg cggcagtgtc tatcggcctt
    19141 gccagttata ttgggatgcc tgtctccaca acacacgtcc tctcgtctgc ggtagcaggg
    19201 acgatggtgg tggacggcgg tgggttacag cgtaaaacgg taaccagcat cctgatggcg
    19261 tgggtgttta ctttaccggc ggcaattttt ctttctggtg ggctgtactg gatagcattg
    19321 cagttgattt aaccttcctg aaaatgcccg gtcctggcga tcgggcattt ccatttttga
    19381 ctagtgataa ccacgcgcgg tcataaaatc cgtaatcgct ttttctgcat caaccaacac
    19441 ctgttccaga ggctgattgg catcgatatc aaccagttgt gcaccaccaa aggttaactg
    19501 tggcgttatg gcaatcttcc tcgccagcga ttcccgttta tggtcgggtt tacgtgcaca
    19561 ggcaacttca aggtcaacat tgagtttgat gaccagatca ggcttatggc tcgccatcca
    19621 gtgaaacgct ttacgttcct ggcttgccag ccatgagaca aaacgaccac cttcaacgtt
    19681 aggtgggaac accgtaccat cgtaagcgcc aggaatttgg tcctgaggat aacggtcggt
    19741 tagaacaatt aacccgcgac gacgacaggc aagcatatga cgaaagcgca gtaagcgacg
    19801 ggcgacaaac gctgtaatta ccagcgccgg aactggtcca ggcaattttt ttgctgtttt
    19861 cacctgattt cgttcaattg ttttatgtaa ggattttccc atcaaaggta attttgtcac
    19921 tgcacgaccg acatttccgg cctgttttcc taaatgaact ctttcggcag caccatattt
    19981 ttcgacaacg gtaataagat gttcacacac cgttgacttg cctgaaccat cgctaccaat
    20041 aacggcaatt aatggaggtg ttattgactg catgaatata tcctttaatt taaatatcca
    20101 ttaaaaatat ttatttggtt aatatgtttt tatgaaagcg taattcaggt caatgtcaca
    20161 attaaccatt gtcacaataa ggttgaacgg atattctgag cacggaccac gtaatattca
    20221 atacattatt tactgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca
    20281 cgccagtgcg tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg
    20341 caagcctggt aaatgaactg gcagcaagaa tgccaacaga acacatttat ctcgggcaat
    20401 cgtccgggcg aattggcgaa tggatttcac agctccctgt tattggcgca ccttgtgggt
    20461 gttatctgcg aagtaaagcg gcacatgtac acgaaaagcc ctcaacaccg cctggcaata
    20521 ttactgcgct ggttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt
    20581 tgtgtaaaag ccagcaaggc tatctgctca tcaccgaccg ctacccgcag gttgaagtgc
    20641 cgggttttcg ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa
    20701 aaatgttaag gcagcgcgag ttgaagctgt accaatggat ggcgtcttat ttacccgtat
    20761 tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc gcgtaaacct gaccaccaac
    20821 tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggcgccaaga
    20881 tccttgaact ggatggtcgg caaccagccg atgaaattat gcaagcgtca ctacgcgcaa
    20941 ttcacgccgc gctttcctga tcaccaaaat ggatagggga tgatttaacg aatggatgca
    21001 ctacaaacac aaactgttaa tagtacaacc gcgccgcagc ccaactacat tccggggctg
    21061 attgcggtgg tcgggtgtga tggcaccggt aaatccacgc tgaccaccga cctggtgaaa
    21121 tcgctgcaac aacactggca aaccgagcgc cgctatctgg ggctgctctc cggcgaagac
    21181 ggagacaaaa tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg actggcggcc
    21241 aaatcctcga aaacccaaag catgaaaacc aaatctccgg cgctatgggc ggcggtgatt
    21301 atgtactgct tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa
    21361 agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat
    21421 gacggaccgg ggatcggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag
    21481 cgcgaacgga gtttatacca aaaaatggcg caataccgtc ctgaattaat cattcgtttg
    21541 gggattgata ttgatactgc catctcccgc aagcctgacc atgagtatgc cgagctgcag
    21601 gacaaaatcg gcgtcatgtc gacgattggc tataacggca caaaaattct tgagattgat
    21661 tccagagcgc cctacagcga agtgctggaa caggtacaga aggcggtttc tctggttgcc
    21721 atcgtttctg accgccgaag tttaacttaa ggctggcggg atatttttta ctatcaacga
    21781 taaggtcagg ctgaattggc tggttttaac atcaaacatt ggtttgcaga tggcgcgttt
    21841 cgcaccatta ttcgcaatag cgcctggtta ggctccagta atgtcgagag cgccttgctg
    21901 gggctgttgg cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt tggtgtactg
    21961 gtgattgtgc aatcgtacgc caagtcgatc agcgatttta ttaagtttca gacatggcag
    22021 ctggtggttc agtacggaac gccagccttg accaacaata atccgcagca atttcgcaat
    22081 gtcgtctcat tttccttctc gctggatatc gtcagcggcg cggtggcgat tgtcggcggc
    22141 attgccttac tgccattcct ttcccattca ttaggtctgg atgaccaaag cttttggctg
    22201 gcggcgctgt attgcacgct cattccttca atggcttcct ccacgccgac cggcattctg
    22261 cgtgcggtag atcgctttga tttgattgct gtacagcagg cgacgaaacc ttttctgcgc
    22321 gcagcgggga gcgtcgcagc ctggtatttt gactttggtt ttgcgggttt tgttattgcc
    22381 tggtacgtgt cgaatctggt tggcggcacc atgtactggt ggtttgccgc gcgcgaatta
    22441 cgccgtagaa atatccataa cgccttcaaa ttgaatctgt ttgagtctgc ccgacacatt
    22501 aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc actccatctg gtcggcgcgt
    22561 aactcgtgca gcacagtgtt ggtggggatc gtgttaggac ccgctgccgc tgggttattt
    22621 aaaatcgcca tgacattctt cgacgccgcc ggaacgccag cgggtttgct gggtaaaagt
    22681 ttttacccag aggtgatgcg tttagatccg cgcaccacca gaccgtggtt gttaggtgtg
    22741 aagtccggtt tactggcggg cggaatcggt attctggtgg cgctcgcggt gttgatcgtc
    22801 ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta tgacctgatt
    22861 caggtgatgc tgggcgcaat tgtgatttcg atgctgggct tcccgcagga gtcattgcta
    22921 ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc aatcggctac
    22981 atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc tgggagcagc gtttgcttac
    23041 ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc caaccttaaa ggcatttttt
    23101 cagcgccatt ccttgcttta taacgcagcc ggagagaaat cctgatgatg agaaaatatt
    23161 tcccgcttga ggcgagcgag cgtctatttg ttgcgattga agaagatgat gtcgttgatg
    23221 cacaggttag tttgccgccg actattgcac taagttgtac caccgaaatc attcatgata
    23281 attacgcgtt atgcctgcaa ttctggctca atggcgttga tcgtcaggaa ttactgcgtt
    23341 tagttcgcaa acaagccaaa ggcgatgagc tgactgctga tgagcgcaaa caatttaaat
    23401 acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg gctgtattta aagaagcatc
    23461 aggccggatt tttattcggc aaaacgaccg tttttctggg gcgttttcag gacggctttc
    23521 gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct gttacggatt tatttaagct
    23581 ctccggtctg gtcgttggtg aactattcat tacgccatag ccagttagaa agcgtaagca
    23641 gttttattgc ctatcggcaa aagcaaatgc ataccctgaa agagattatt gccaaaccgc
    23701 gactaaccgg cagggaattt catgatgtgc gaaaaatcat cagccagcag gtttcttatt
    23761 acgacacctt aagatcgctt gatcctgaaa ataaagaggc gttacagatt tcccgtttcc
    23821 tcgccgccat taatggcctg atgggagata aacacgacga catggtggcg gatgatatgg
    23881 aaaaccgcca gtcgtattat gcaccgttgg cgctggatag cgatatttgt cagcgcttag
    23941 agctgctgat ttcgcgtttt ccgctatagc cggagcaatg atgtcatctc gtttgattat
    24001 tgcgttgatt atcatgctct tagccccggg tgtgcaggca cacaatttcg tcactggcaa
    24061 aacggttacc cctgtttata tccaggaagg cggcgagctg cttctgaata gcgacgacga
    24121 aattcactac caaaagtgga acagcacaca attagcggga aaagtccgca ttattcagta
    24181 tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg ttaattaaag ctgttgaagc
    24241 ggcaaacttt ccgcaggatc gttttcagcc caccaccatt gtgaacaccg atgatgccat
    24301 ctttggtact ggctattttg ttgttggcaa aatcgaaaaa aataaacgcc gctatccgtg
    24361 ggcgcaattc gttattgacg gcaatggaca gggacgcgtt gcctggcgat tgccggagca
    24421 gagttcaacc attctggtgc tgaataaagc cgggcaaatt cagtgggcaa aggacggcag
    24481 cctgacgccg gaagaggtag atcatgtcat tgcgttggcg cagaaactga gcaatgaata
    24541 aaaaactcaa aggattgagg gcgttagtga gatgaagctg gttgaacact acatcatgcg
    24601 tggtacgcgc cgtctggtgc tgattatcgt cggttttctg atctttattt tcgccagcta
    24661 ctccgcacag cgttatctga ccgaagcggc aaatgggaca ttagctcttg atgttgtgct
    24721 ggatatcgtt ttctacaaag tactgattgc actggagatg ttgttacctg ttggtctgta
    24781 tgtgtcagtt ggcgtgacgc tagggcagat gtataccgac tcggaaatta ccgctatctc
    24841 tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt ctttatctgg cgataccgct
    24901 aagtattttt gtcacccttc tgtcgatgta tggtcgtccg tgggcttatg cgcagattta
    24961 tcaactggag cagcagtcgc agtcggagct ggatgttcgc cagttgcggg caaagaaatt
    25021 taacactaac gataacggac gaatgatcct ttcgcagacg gttgatcagg ataataatcg
    25081 cctgactgac gcgctgattt atacttctac tgccaatcga acccgtattt tccgcgcccg
    25141 ttcggttgat gtggttgacc catcacccga gaaaccgacc gttatgttgc acaacggcac
    25201 tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa cagatctacc gtaatctgca
    25261 attacatctg aatccgctgg atcaaagccc gaacgtcaaa cgcaaagcga aatcggtcac
    25321 ggagctggcg cgctccgcct ttcctgccga tcatgccgaa ctgcaatggc gacaaagccg
    25381 tggcctgacc gcattgttga tggcgctgct ggccatttca ttaagtcggg taaaaccgtg
    25441 gcaagggcga ttttcaacgt tattgccact gacgttgctg tttattgcca ttttttatgg
    25501 cggcgacgtc tgccgtacac tggtggctaa cggcgcgatt cccctcattc ctggtttgtg
    25561 gttagtgccc ggactcatgc taatgggcct gctgatactg gtcgcacgtg acttctcttt
    25621 gctgcagaaa ttttcccgat gaatgttttc agtcgctatt taatccgtca tctctttctc
    25681 ggttttgccg ccgctgcagg gctattgctg ccgcttttta ccaccttcaa cctgattaac
    25741 gaactggatg gtgtcagccc gggcggttat cgctggactc aggcggtgct ggtggtgcta
    25801 atgaccttac cgcgcacact ggtcgaactt tcgccgttta tcgcgttatt gggtggtatt
    25861 gttggactgg ggcagttatc gaaaaacagt gagcttaccg ccattcgcag cacggggttt
    25921 tctatcttcc gtattgcact ggtggcgctg gttgccggga tattgtggac tgtttcgtta
    25981 ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac aggcgttgca aatcaaatcg
    26041 accgccaccg cgttggggga ggacgatgac attaccggca atatgctgtg ggccaggcgt
    26101 ggtaatgaat ttgtgacagt gaaatcgctg aacgagcagg gccagccggt gggcgtggag
    26161 atatttcatt atcgcgacga tctctcgctt gaatcctaca tttatgcacg cagtgccacc
    26221 attgaagacg acaaaacgtg ggtcctgcat ggtgtgaatc ataaaaaatg gctgaatggc
    26281 aaagaaacgc tggaaacatt agataatctt gcctggcaat cggccttcac cagtatgaat
    26341 cttgaagagt tatcaatgcc ggggaatact ttttctgtcc gtcagcttaa tcattacatc
    26401 cattatttgc aggaaaccgg gcaacccagc agcgaatacc gccttgctct gtgggaaaaa
    26461 ctggggcaac ccatcctgac cctggcgatg attttgctgg ctgtgccgtt tacctttagt
    26521 gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag gcgtcatcgt gggcttactc
    26581 acctggatca gctatcaaat catggttaat ctgggattgc tatttgcgtt aagcgcacct
    26641 gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg cgttgagcct ggtgtattgg
    26701 tatgacagac aacattaaac ccgccgtggc gcaaacacca cggcgggctc acattactgc
    26761 ttcagagtag caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcattt
    26821 acgctacagc gcagcagcgt gatgcctgca ggcgcggcag gcggcaggat aagattcaca
    26881 tacacgcccg gcgagataag ctcgcgccag atgcgtaaac cttcctcttt cgaaccgatt
    26941 attactggca ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg
    27001 tcatacagat ggttggcgtt atccatcagc ttttgccgca gctctggttg tgtggcaata
    27061 gttcttaacg acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata
    27121 tacgggcggc tgccgtagcg cagcacttcc atcgcttccg aaccgacagc aaaaccgcca
    27181 atcgaggcca gacttttact gaaagtacca acgataatat ccacatcatc ttcaacgccg
    27241 acagcttcag ccagtccgcg ccccgttgcg cctaacacga caaacgaatg cgcttcatcg
    27301 acaatcaaat aaccaccgag acgacgtttg atatcaacaa tttccgccag cggcgcaacg
    27361 tcaccgagca tgctgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg
    27421 aggcgaacca tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttcc
    27481 gcgccaccga gagaacaggc gtcataaata ctggcgtggc tgtcggcatc cagtaacacc
    27541 acggcgttat ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa
    27601 aaaacgatag cggtagggcg attgaaaaac tcggcaatct ctttctctaa cgccaggtgc
    27661 ggcgcataac tgccgtttgc catacgagaa ccggtggtgc cggtgccttg ggccgctaac
    27721 gcggcctgac cttcgctgat ggcatcatga ttaaaggtta aaccgagata attattggtc
    27781 ccggccagaa tcactttttt attgccgatc cggccttcgg tggcggaata cacttcatca
    27841 atacaggtgc caaacggtgt taacccgttg tcactaaatt gcagacgctc gccagcaagg
    27901 cgcgcatatt tatcgtatag ccccattatt tattctccag ccatggaagt agagtttcca
    27961 ttaattgcgc agggggtttg acattcagca gaatattaat aggaatggaa atatcgaatc
    28021 tgtcttccag catcattaag agatccatca ctttgatcga ctccaggcca agatcgttga
    28081 cgagatcgct gtcgggctta atttccacac cgttttcgac caaatcctgt aaacaggaaa
    28141 ggatgtaatc cattactatt tcacgattta ccatagaaac gttaacacac ctttaaaaca
    28201 agacaatcaa aatagcccgt tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt
    28261 gatatcttca gaaatacggt tattacttgc cgaccagtcg gcgtgggtta attcacgaat
    28321 tttcgagcgg gtcagcatag gttctttacc tgccaggcga ctcaacgcgg tactgatatc
    28381 cgcaaggcct gtgagtaccg gcagaggaat gtcaaccatt cgcacggaac cacaacgggc
    28441 tttggcggca agctgtcgta cgcgttgcca gtcatagcca ccggcgacgc catcgcataa
    28501 ttcataggtt tgcgtctgta cagtttcggc gcttaaccac tgacccactg cttgcgcaaa
    28561 atcggtgacg tgaagaaaag agagttgtgt atctggcgca ccaagtcgtg gcagcaggcc
    28621 gcgtagcatc cagtcaaaca gcggttttaa ctctttatcg ccgggaccat acacggctgt
    28681 cgggcgaaaa acgcccagcg taatttcgtc cgccattgct gccagtcgtt gctcggcgac
    28741 gtatttggat tttgcatacc aggagagctc gggatggcgc gccgccagcg aagagataaa
    28801 cagaaaacgt tggcaaaagc cgctctcttt tgctgcttgc atcaggcgca ggctgccgtc
    28861 aacgttacag cgggtgaaaa tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac
    28921 cactacgctg gctccggcaa ccagctcgct aagtgaatga gtatcttcca gcgaaccgcg
    28981 cacccaggtg agattatcat tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg
    29041 aaagccgcgg gcgagcaggt tatcgataat atatttaccg ataaacccgg tagcgcccgt
    29101 caccgcgaca gtttgattca tgccagggat tcctgcacat taagactggc agcataagcc
    29161 ttctgataac gttttttcgc ttccgcacgg gcaggcttgc cggaggacgt tcggggaata
    29221 ctgtggggcg gcaacagatc gatagccgcg gtcacgccaa attcgctttg gatccgtgcc
    29281 gccagcgcgt ggataagctg cccgcgacgt tcttcgtcgc tgatccgaca ctggatctgc
    29341 aaaatgattt tttcctgggc ggtaacaaaa gcaatcgcat cgccagaatg aatttccggt
    29401 tcctgttccg ctatatattc aatatcctgc ggccagatat tacggccacg aataataatc
    29461 agatctttaa tgcgtccggt gacatacaga taaccgtcca gcagataacc gaggtcgccg
    29521 gtgtctaacc agcccgtcgc ggcaatctcg tcttgcgaaa cctggtcgcc aaagtaaccg
    29581 ctcatcagac tgggaccgga gatgcaaata tggcctacca cacgttccgc gaccggcata
    29641 cctgcttcat tgcggatttc aataccatgt tccggcaacg ctttgccgca gttgacgaaa
    29701 gtcgatacgg cgcgtgtctc tgcacccggc gcgacggctt taccctgata ttcgaggatg
    29761 tcgcgatcca cttcgttaac cacaaccccg gaggcttcat cagagaagct gacagccagc
    29821 gcattttctg ccagtccgta gcacggcatg aaagttttat tgtcaaagtt aacctgacgg
    29881 aaacattcag cgaattgatg gagttgttct gcggagatcg gctcagcacc aataccagcg
    29941 acgcgccagt aggaaagatc cagttcagcg agatcttttt cattcacgcg gcgttggcac
    30001 aattcatagc caaacggcgg cgcaacggaa acggtgccgc gatttttact gatcaattta
    30061 agccattgca aaggacgcat ggcaaaatcc tgagtgcgca aataatctac tgaaagttgc
    30121 gtggcgacgg ggggcaggag aaagccgacc agtcccatat catggtagaa aggctgccag
    30181 gagacgcagc ggtcgctagg gcgtaattta atgccatcgt ggcttatagc acgtagatta
    30241 gccatcactt cgcgatgggt gataatgacg ccacggggaa aacgggtgct gccggaggtg
    30301 tactggaggt aggcgatatc gttcggaact ggacgctgga gcgcaacatc ggcttccggt
    30361 aatgccttaa accaagcgtg gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca
    30421 ttgaccagtg gcaactactc atcaccagtg ataatggctg cgggctggca acttgccagt
    30481 aaaccctaga gtttggcgct ccaggaatcc cgctgaccga cgcccattgg aatcgccaac
    30541 gggaaggcga ctaagccagc atactgacag gcaaaaaaag cctctacaaa cccgctactc
    30601 gtttcggcaa tcagcgccac gcgttcgcct tttttcaggt tcagcgataa caaccgcttc
    30661 gcaccagctt cggcacgcgt ttttaatgtc tgatattcca gttgatcttc aagttggcaa
    30721 cgtctgtcat aaaaattcat tccggctcta ctcagagcgg cgtagtccaa agcatcaacc
    30781 agcgttggaa aatcggcata gcgcataggt agggaatgcg taaagatttt attagacata
    30841 tacaccacga aaaaattgga tatcaaaaat aaaatgacaa taatgcggtg gattatatag
    30901 gcataaaaga taaatgagat agtattttca tcggtgtgaa aatgaacatt gatttaaata
    30961 atgagtttga gttttttatt tttattatga atgatattta atttatgaat atattaagaa
    31021 gaagccttgt ccggtcgcct taaatcgaca aaaggcattg aggcgtttct gtgtagcctc
    31081 tgaccttatc ataatcatta attaatacta tttattcgtc gtttgtattc attgagttga
    31141 ttattaagat gccgctcgtt gctgttgcaa cctgtttttc accattaatt atttgttatc
    31201 cacagagata tggaatgcga tctgctgatg attaaaattg aaaaagtaat taataaaaat
    31261 gaccttaagg catttatcgc ttttccgtca tcactttatc ccgacgatcc aaactggata
    31321 ccccctttat tcattgaacg caacgagcat ttgtctgcga aaaatccggg gacggaccat
    31381 atcatctggc aggcgtgggt ggcaaaaaaa gcggggcaga tagttgggcg tattaccgcg
    31441 caaatagata ccttgcaccg tgagcgttac ggtgaagata ccggtcattt cggcatgatt
    31501 gacgccattg atgatccgca ggattttgcc gcgttgtttg gcgcagcgga agcgtggttg
    31561 aagtcacaag gtgcaagtaa gatcagcggg cctttcagcc tgaatatcaa tcaggaaagc
    31621 ggattactga ttgaaggttt tgacacacca ccctgtgcga tgatgccaca cggcaaaccg
    31681 tggtatgccg cgcatattga acaactgggt tatcacaaag gcattgattt actggcgtgg
    31741 tggatgcagc gaaccgatct cactttctct ccggcgctaa aaaaactgat ggatcaggtg
    31801 cgcaaaaagg tgaccattcg ctgcatcaat cgtcagcggt ttgccgaaga gatgcagatc
    31861 ctgcgtgaga ttttcaactc tggctggcag cacaactggg gatttgtgcc gtttaccgaa
    31921 catgaattcg cgaccatggg cgaccaactt aaatatctgg tgccggacga tatgatctac
    31981 atcgctgaga ttgattctgc accctgcgcg tttattgtcg gcttgcctaa catcaacgag
    32041 gcgattgccg atctgaacgg atcgctcttt cccttcggct gggcaaaatt gctgtggcgt
    32101 ttgaaagtca gcggtgtgcg aactgcgcga gtaccgctga tgggcgtgcg cgacgagtat
    32161 cagttcagcc gcatcggccc ggtgattgcg ctgttattga ttgaagcctt acgcgatccg
    32221 tttgcccgcc ggaagatcga tgcgctggag atgtaatgga tcctcgaaac caatacaggc
    32281 atgaataaca tgctggaacg cattggggcg gagccgtaca agcgttaccg attgtatgaa
    32341 aagcaaattt aataccagat cctgtttagc gcaggggcgg tcattcggga gagggtatag
    32401 atgaacagat ttaacaccag atcctgttaa gtgcaggggc ggtcccctcg ccttggggat
    32461 agggtaaaac tctgctcaca ttcccacctt gttcatctcc agcggcaccg ccgagcgaat
    32521 caaatggtgg ccttccagct cgatatcgtg gagattcttt ttcacggtcc gtacatgatc
    32581 gcgcgctgcg cgctgagcga cgtgcggcag ccgctgcaac accgcgttgt agatccgcgc
    32641 atgctggcga tcgatctgct ttttttgctg tggtcgatgg tagagattat ttacgctggc
    32701 aaacactgaa ttaaacatca gatcggtcaa tgattgcagc gtaaacacca gcacctgatt
    32761 gtgagaagcc tgacaaatgg cgagatggaa agcgtgatcc aactgcgcat gttcgatcag
    32821 cgaaatctct ttgtggttct cactggcggc gagcattttt tcataacagc gggttatcac
    32881 aacaaaatca gcctgcgttc ccagcgttgc cgccagcctt gccgattcgc cctccagtaa
    32941 tgcgcgaacg tcgagcagat cgtacagcgt tcgcggctgc gtactgaaca gatggatcag
    33001 cgggctggtg tcctgcaccc gattaagccg tgcgacatga gaatcgcgac cctgcgccgt
    33061 ttcaataatc ccgcgcccgc gcagcacggt cagcccttca cgcagtgcgg agcgtgagaa
    33121 gccgagcttt tcacacagtc gacgttccga gggaagcggc tgaccgacct tcagtacgcc
    33181 gtcgataatt aaccgttcga tactctctgc aaccacttcg caaatagggc gacgttcatc
    33241 tttcattccc ggacctcgtg cacaggtagg accaattttt ctgttcttaa ctcgcaaaac
    33301 acgcacatca cgcaagtgat tatgttacat caatttaaca ttaagttaac caagacaagg
    33361 tcacagagct ggaaaaaaaa tggtctgacc ggtagctaaa gagatagacg aaaacgaaaa
    33421 gcccgcttaa taactgttca cagaagcagc gcgcaaaaat cagctgccac acaacaaagc
    33481 gaagcctact catgagcatc ttgtacgaag agcgtcttga tggcgcttta cccgatgtcg
    33541 accgcacatc ggtactgatg gcactgcgtg agcatgtccc tggacttgag atcctgcata
    33601 ccgatgagga gatcattcct tacgagtgtg acgggttgag cgcgtatcgc acgcgtccat
    33661 tactggttgt tctgcctaag caaatggaac aggtgacagc gattctggct gtctgccatc
    33721 gcctgcgtgt accggtggtg acccgtggtg caggcaccgg gctttctggt ggcgcgctgc
    33781 cgctggaaaa aggtgtgttg ttggtgatgg cgcgctttaa agagagcctc gacattaacc
    33841 ccgttggtcg ccgcgcgcgc gtgcagccgg gcgtatgtaa cctggcgatc tcccaggccg
    33901 ttgcgccgca taatctctat tacgcgccgg atccttcctc acaaatcgcc tgttcaattg
    33961 gcggcaatgt ggcggaaaat gccggtggcg tccactgcct gaaatatggt ctgaccgtac
    34021 ataacctgct gaaaattgaa gtgcaaacgc tggacggcga ggcgctgacg ctgggatcgg
    34081 acgcgctgga ttcacctggt tttgacctgc tggcgctgtt caccggatcg gaaggtatgc
    34141 tcggcgtgac caccgaagtg acggtaaaac tgctgccgaa gccgcccgtg gcgcgggttc
    34201 tgttagccag ctttgactcg gtagaaaaag ccggacttgc ggttggtgac atcatcgcca
    34261 atggcattat ccccggcggg ctggagatga tggataacct gtcgatccgc gcggcggaag
    34321 attttattca tgccggttat cccgtcgacg ccgaagcgat tttgttatgc gaactggacg
    34381 gcgtggagtc tgacgtacag gaagactgcg agcgggttaa ctacatcttg ttgaaagcgg
    34441 gcgcgactga cgtccgtttg gcacaggacg aagcagagcg cgtacgtttc tgggccggtc
    34501 gcaaaaatgc gttcccggcg gtaggacgta tctccccgga ttactactgc atggatggca
    34561 ccatcccgcg tcgcgccctg cctggcgtac tggaaggcat tgcccgttta tcgcagcaat
    34621 atgatttacg tgttgccaac gtctttcatg ccggagatgg caacatgcac ccgttaatcc
    34681 ttttcgatgc caacgaactt ggtgaatttg cccgcgcgga agagctgggc gggaagatcc
    34741 tcgaactctg cgttgaagtt ggcggcagca tcagtgggga acatggcatc gggcgagaaa
    34801 aaatcaatca aatgtgcgcc cagttcaaca gcgatgaaat cacgaccttc catgcggtca
    34861 aggcggcgtt tgaccccgat ggtttgctga accctgggaa aaacattccc acgctacacc
    34921 gctgtgctga atttggtgcc atgcatgtgc atcacggtca tttacctttt cctgaactgg
    34981 agcgcttttg atgctacgcg agtgtgatta cagtcaggcg ctgctggagc aggtgaatca
    35041 ggcaattagc gataaaacgc cgctggtgat tcagggcagc aatagcaaag cctttttagg
    35101 tcgcccagtc accgggcaaa cgctggatgt gcgttgtcat cgcggcattg ttaattacga
    35161 cccgaccgag ctggtgataa ccgctcgtgc cggaacgccg ctggtggcga ttgaagcggc
    35221 gctggagagc gccgggcaaa tgcttccctg tgagccgccg cattatggtg aagaagccac
    35281 ctggggcggg atggtcgcct gcgggctggc ggggccgcgt cgcccgtgga gcggttcggt
    35341 ccgcgatttt gtcctcggca cgcgcatcat taccggcact ggaaaacatc tgcgttttgg
    35401 tggcgaagtg atgaaaaacg ttgccggata cgatctctca cggttaatgg ccggaagcta
    35461 cggttgtctt ggcgtgctca ctgaaatctc aatgaaagtg ttaccgcgac cgcgtgcctc
    35521 tctgagcctg cgtcgggaaa tcagcctgca agaagccatg agtgaaatcg ccgagtggca
    35581 actccagcca ttacccatta gtggcttatg ttacttcgac aatgcgttgt ggatccgcct
    35641 tgagggcggc gaaggatcgg taaaagcagc gcgtgaactg ctgggtggcg aagaggttgc
    35701 gggtcagttc tggcagcaat tgcgtgaaca acaactgccg ttcttctcgt taccaggtac
    35761 cttatggcgc atttcattac ccagcgatgc gccgatgatg gatttacccg gcgagcaact
    35821 gatcgactgg ggcggggcgt tacgctggct gaaatcgaca gccgatgaca atcaaatcca
    35881 tcgcatcgcc cgcaacgctg gcggtcatgc gacccgcttt agtgccggag atggtggctt
    35941 tgccccgcta tcggctcctt tattctgcta tcaccagcag cttaaacagc agctcgaccc
    36001 ttgcggcgtg tttaaccccg gtcgcatgta cgcggaactt tgaggagcag gctatgcaaa
    36061 cccgattaac cgaagagatg cggcagaacg cgcgcgcgct ggaagccgac agcatcctgc
    36121 gcgcctgtgt ccactgcgga ttttgtaccg caacctgccc aacctatcag cttctgggcg
    36181 atgaactgga cgggccgcgc gggcgcatct atctgattaa acaggtgctg gaaggcaacg
    36241 aagtcacgct taaaacacag gagcatctcg atcgctgcct cacttgccgc aattgtgaaa
    36301 ccacctgtcc ttctggtgtg cgctatcaca atttgctgga tatcgggcgt gatattgtcg
    36361 agcagaaagt gaaacgccca ctgccggagc gaatgctgcg cgaaggattg cgccaggtag
    36421 tgccgcgtcc ggcggtcttc cgtgcgctga cgcaggtagg gctggtgctg cgaccgtttt
    36481 taccggaaca ggtcagagca aaactgcctg ctgaaacggt gaaagctaaa ccgcgtccgc
    36541 cgctgcgcca taagcgtcgg gttttaatgc tggaaggctg cgcccagcct acgctttcgc
    36601 ccaacaccaa cgcggcaact gcgcgagtgc tggatcgtct ggggatcagc gtcatgtcag
    36661 ctaacgaagc aggctgttgt ggcgcggtgg actatcatct taatgcgcag gagaaagggt
    36721 tggcacgggc gcgcaataat attgatgcct ggtggcccgc gattgaagca ggtgccgagg
    36781 caattttgca aaccgccagc ggttgcggcg cgttcgtcaa agagtatggg cagatgctga
    36841 aaaacgatgc gttatatgcc gataaagcgc gtcaggtcag tgaactggcg gtcgatttag
    36901 tcgaacttct gcgcgaggaa ccgctggaaa aactggcaat tcgcggcgat aaaaaactgg
    36961 ccttccactg cccgtgtacc ctacaacatg cgcaaaagct gaacggcgaa gtggaaaacg
    37021 tgttacttcg tcttggattt accttaacgg acgttcccga cagccatctg tgctgcggtt
    37081 cagcgggaac atatgcgtta acgcatcccg atctggcacg ccagctgcgg gataacaaaa
    37141 tgaatgcgct ggaaagcggc aaaccggaaa tgatcgtcac cgccaacatt ggttgccaga
    37201 cgcatctggc gagcgccggt cgtacctctg tgcgtcactg gattgaaatt gtagaacaag
    37261 cccttgaaaa ggaataaaaa aatgaaaact aaagtcattc ttagccagca aatggcgagt
    37321 gcaattattg ccgcaggtca ggaagaggcg cagaaaaata actggtctgt ttccattgct
    37381 gttgccgatg acggcggtca tctgctggcg ttaagtcgca tggacgattg cgcgccgatt
    37441 gcggcttaca tctcccagga gaaagcgcgt accgccgcgc tggggcgtcg tgaaactaag
    37501 ggctatgaag agatggtgaa caacggacgt accgcgttcg tgactgcgcc gttactgacg
    37561 tcgctggaag gcggcgtacc ggttgttgtt gatgggcaaa ttattggtgc cgtgggcgtt
    37621 tctggtttaa ccggagcaca ggatgcgcag gtcgcgaaag cggcagcagc ggtgttggcg
    37681 aaataagcga aaacgaggag agaaacaatg agtcaaacca taacccaggg ccgtttacgc
    37741 attgacgcca attttaaacg ttttgtggat ggagaagttt tgccgggcgt ggaactggac
    37801 gctgctgcat tctggcacaa tgttgatgag atcgttcacg atctggcacc agaaaatcgt
    37861 cagttgctgg cagaacgcga tcgcattcag gcggcgcttg atgagtggca tcgcagcaat
    37921 ccggggccgg taaaagataa agcggcctat aaatctttcc ttcgtgaact gggctacctg
    37981 gtgccgcaac cggagcgcgt gacggtggaa accacgggca ttgacagcga aatcaccagt
    38041 caggcggggc cacagctggt ggttccggca atgaacgccc gctacgcgct gaacgcggcg
    38101 aacgctcgct ggggctcact gtacgatgcg ttatacggca gcgacatcat cccgcaggaa
    38161 ggggcgatgg tcagtggcta cgatccgcag cgcggtgagc aggttatcgc ctgggttcga
    38221 cgtttcctcg atgaatcact accgctggaa aacggcagct atcaggatgt ggtggcgttt
    38281 aaggtggtcg ataaacaatt acgcatccag ttgaaaaatg gtaaagaaac cacgttacgt
    38341 actccagcac agtttgtcgg ttaccgtggc gatgccgctg cgccgacctg cattttgctg
    38401 aaaaataacg gcctgcatat tgaactgcaa attgatgcca acgggcggat tggcaaagac
    38461 gatccggcgc acatcaacga tgttatcgtc gaagctgcta tcagtaccat tctcgactgc
    38521 gaagattcgg tcgcggcggt tgatgcggaa gataaaatcc tgctgtaccg caatctgctg
    38581 ggcctgatgc aggggactct gcaagagaaa atggagaaaa acggtcggca aatcgtacgt
    38641 aaactgaatg acgatcgtca gtacaccgcc gccgatggct ctgaaatttc tctgcacgga
    38701 cgctcgttgc tgtttatccg caacgtgggt catttgatga ccattcctgt gatttgggac
    38761 agcgaaggca atgaaatccc ggaaggtatt cttgatggcg tcatgactgg cgcgattgcc
    38821 ctctatgatt taaaagtgca gaaaaactcg cgcactggca gcgtctatat tgtgaaaccg
    38881 aaaatgcacg gtccgcagga agtggcgttc gccaacaaac tgtttacccg cattgagaca
    38941 atgctcggta tagtaccgaa taccctgaaa atgggcatta tggatgaaga acgccggacc
    39001 tcgctgaact tgcggagctg tatcgctcag gcgcgcaacc gcgtggcgtt catcaatacc
    39061 ggtttcctcg accgtaccgg cgatgaaatg cattcggtga tggaagctgg cccgatgctg
    39121 cgtaaaaacc agatgaaatc gacgccgtgg atcaaagcct acgaacgtaa taacgtgctt
    39181 tccggtctgt tctgtgggct gcgcggtaaa gcgcaaatcg gtaaaggcat gtgggcaatg
    39241 ccggacctga tggcagacat gtacagccag aagggcgacc aactgcgtgc cggggcaaac
    39301 acagcctggg ttccgtcacc aaccgctgct acgctccatg cgctgcacta ccaccaaacc
    39361 aacgtacaga gcgtgcaagc caacattgcc cagaccgagt tcaatgctga atttgaaccg
    39421 ctgctggacg atctgctgac tattccggtg gctgaaaacg ctaactggtc ggcgcaagag
    39481 atccaacaag agctggataa caacgtgcag gggattctgg ggtacgtggt gcgttgggtg
    39541 gagcagggga ttggttgttc aaaagtgccg gatattcaca acgtggcgct gatggaagac
    39601 cgcgcaacgc tgcgtatctc cagccagcat atcgccaact ggttacgtca cggtattctg
    39661 accaaagaac aggtgcaggc gtcgctggag aatatggcga aagtggttga tcagcaaaat
    39721 gctggcgatc cggcttatcg tccgatggcg gggaatttcg ctaactcgtg tgcttttaaa
    39781 gctgccagcg atttaatctt cctcggcgtg aaacagccaa acggttatac cgaaccgtta
    39841 ttacacgcct ggcgtttacg cgaaaaagaa agtcattaat agcgtcaaaa aagccccggc
    39901 aagttttatt gtcggggcta tgatagtaac tgcggtagta catttatccc ggaaataacc
    39961 ggagcgatta tcagggttta acaatattaa atacccgtag cgcattacta gcgggcctaa
    40021 aacaacaata tggacatctt aacgatactc tgcgcctgcg tgtgcggaac agaaaccctt
    40081 acccgatata cttgttatgc cagaggtagc aattgcgtta gcggcgtact gttgctgtaa
    40141 agtataacgc ctatatttta cagttcagtc ctcttttgct tattacaacc ttaagtatat
    40201 caagcatata aagataataa gagactgaac aatatggtta cctggaccca aatgtatatg
    40261 ccgatgggag gactggggct atccgctctg gtcgccctga tcccgataat attcttcttc
    40321 gttgcactcg cggtattacg tctgaaagga catgtcgctg gagcaataac ccttatatta
    40381 tctatcctga ttgcgatatt cgcctttaaa atgccgattg atatggcatt tgctgctgcg
    40441 ggctatggct ttatttatgg attatggcca attgcgtgga ttattgtcgc ggcggtgttc
    40501 ctgtataaat taaccgttgc cagcgggcag ttcgatatta tccgcagctc ggttatctcc
    40561 atcaccgacg atcagcgttt gcaggtgtta ctgattggtt tctcctttgg tgcgttgctg
    40621 gaaggagcgg ctggctttgg tgcgccggtg gcgattaccg gtgcgctgct ggtgggcctg
    40681 ggcttcaaac cgttatacgc ggcggggctg tgtctgattg ccaatactgc gccggtggcg
    40741 tttggtgcgt tgggcgtgcc gattctggtc gccggtcagg taacgggaat cgatccgttc
    40801 cacattggcg caatggcggg acgtcagtta ccgttcctgt cggttcttgt gccgttctgg
    40861 ctggtagcaa tgatggacgg ctggaaaggg gtgaaagaga cgtggccagc ggcgctggtt
    40921 gctgggggaa gcttcgctgt cactcagttc tttacctcta actatattgg tccggaactg
    40981 ccggatatta cttcggcgct ggtgagtatc gtctcactcg ctttattcct taaagtctgg
    41041 cggccgaaaa ataccgaaac ggcaatcagc atggggcaat ccgcaggtgc gatggtggta
    41101 aataagccat cttctggcgg tcccgtgcct tcagaatata gtctggggca aatcattcgt
    41161 gcgtggtcac cgtttttaat cttaacggtg ctggtcacca tctggaccat gaagccgttt
    41221 aaagcgttat ttgctccggg cggcgcgttt tattcactgg tgattaattt ccagatccct
    41281 catttgcatc aacaagtgtt gaaagcggca cccattgtcg cccaaccaac gccaatggat
    41341 gcggtgttta aattcgaacc tctctcggct ggcggcaccg ctatttttat tgcggcgatt
    41401 atctctatct tcatcctcgg cgtgggcatc aagaaaggga ttggcgtctt tgccgaaacg
    41461 ctaattagtc tgaagtggcc gatattgtcg attggcatgg tgctggcatt cgccttcgtc
    41521 accaactatt ccggcatgtc caccacgctg gcgctggtac tggcaggtac aggcgtgatg
    41581 ttcccgtttt tctcaccgtt tctcggctgg ctgggcgtat tccttaccgg gtcggacact
    41641 tcttcaaacg ccctgtttgg ttcactgcaa tcgaccacgg cgcagcaaat caacgtctct
    41701 gacactctgc tggtggcagc aaataccagc ggcggcgtaa ctggcaagat gatctccccg
    41761 caatctatcg ccgtggcctg cgccgcgacg ggcatggtgg gccgagaatc tgaactgttc
    41821 cgctacaccg tgaagcacag tctgattttt gccggcgtta tcggcgttat caccctgctg
    41881 caggcgtatg tgtttaccgg gatgttagtc tcgtaaatca ataaagccgg atgaaggtaa
    41941 atatcacccg gctttccatt tgtctgccgt ttatctcgcc agggaaccgt ttttacgatt
    42001 ggcttcttta tacgtagtct ccgtttcacg atgcgaattg tttaatattt atacaacttg
    42061 gcttctgtgc tggttataaa gtacccactt tcatgagatc aattttagta tgttattaca
    42121 ttgcttcatc tgttgctttc atgacgaatg gcgttttttc tcttatttat atttttcact
    42181 agaacaatga gtagatcaaa attcccaaaa ctcccaaaaa cataagatta ttcgcaaagc
    42241 tgattttgca gaataatgag aacttttaag atttgtcgag gatcaatttt aagcaattta
    42301 tctccacttg ttacataaaa gatgccgtta ggttaataag ttaatcttgc tataacagtg
    42361 ttcgttactg cctattaata aagatatttg ggatagggta acgtggatga ggattcgaaa
    42421 tcatccgtct tgcggaaagt gaaatgttgg cagatgttaa tattatgtat gtattttaac
    42481 tccaacaaca caccaatcat tttgttagaa ataagaaaat ttatggttaa ataatttggt
    42541 gattatgaag cattaaatcg taatgccaaa tattgttcgc tttggagagt attattgcct
    42601 gtttgtgtct gttgagcaga aaaatcatct gaatttgaat acttttatta ggcgcgaaga
    42661 aatgaaaaaa tcattaaaat cattaattta tatgaatatt tcagagttgg agttcgaatg
    42721 ttgacacctg agtatttcct gctaaaaata gccctgcttt acggatggtt gtattgcaat
    42781 aacacgaaag
//
LOCUS       DAJTRQ010000018        41013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000018 DAJTRQ010000000
VERSION     DAJTRQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..213)
                     /locus_tag="OO332_001121"
     CDS             complement(<1..213)
                     /locus_tag="OO332_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCR5941680.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAA"
     gene            complement(253..1098)
                     /gene="glpF"
                     /locus_tag="OO332_001122"
                     /pseudo
     CDS             complement(253..1098)
                     /gene="glpF"
                     /locus_tag="OO332_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1524..1769
                     /gene="zapB"
                     /locus_tag="OO332_001123"
     CDS             1524..1769
                     /gene="zapB"
                     /locus_tag="OO332_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5941681.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1854..2339)
                     /gene="rraA"
                     /locus_tag="OO332_001124"
     CDS             complement(1854..2339)
                     /gene="rraA"
                     /locus_tag="OO332_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5941682.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2432..3358)
                     /gene="menA"
                     /locus_tag="OO332_001125"
     CDS             complement(2432..3358)
                     /gene="menA"
                     /locus_tag="OO332_001125"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5941683.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3425..4756)
                     /gene="hslU"
                     /locus_tag="OO332_001126"
     CDS             complement(3425..4756)
                     /gene="hslU"
                     /locus_tag="OO332_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5941684.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4766..5296)
                     /gene="hslV"
                     /locus_tag="OO332_001127"
     CDS             complement(4766..5296)
                     /gene="hslV"
                     /locus_tag="OO332_001127"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5941685.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5389..6348)
                     /gene="ftsN"
                     /locus_tag="OO332_001128"
     CDS             complement(5389..6348)
                     /gene="ftsN"
                     /locus_tag="OO332_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5941686.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6440..7465)
                     /gene="cytR"
                     /locus_tag="OO332_001129"
     CDS             complement(6440..7465)
                     /gene="cytR"
                     /locus_tag="OO332_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5941687.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7621..9819)
                     /gene="priA"
                     /locus_tag="OO332_001130"
     CDS             complement(7621..9819)
                     /gene="priA"
                     /locus_tag="OO332_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5941688.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10022..10234
                     /gene="rpmE"
                     /locus_tag="OO332_001131"
     CDS             10022..10234
                     /gene="rpmE"
                     /locus_tag="OO332_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5941689.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10295..10903)
                     /locus_tag="OO332_001132"
     CDS             complement(10295..10903)
                     /locus_tag="OO332_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5941690.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11087..11404)
                     /gene="metJ"
                     /locus_tag="OO332_001133"
     CDS             complement(11087..11404)
                     /gene="metJ"
                     /locus_tag="OO332_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5941691.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11681..12841
                     /gene="metB"
                     /locus_tag="OO332_001134"
     CDS             11681..12841
                     /gene="metB"
                     /locus_tag="OO332_001134"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5941692.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12844..15276
                     /gene="metL"
                     /locus_tag="OO332_001135"
     CDS             12844..15276
                     /gene="metL"
                     /locus_tag="OO332_001135"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5941693.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15625..16515
                     /gene="metF"
                     /locus_tag="OO332_001136"
     CDS             15625..16515
                     /gene="metF"
                     /locus_tag="OO332_001136"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5941694.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16844..19024
                     /gene="katG"
                     /locus_tag="OO332_001137"
     CDS             16844..19024
                     /gene="katG"
                     /locus_tag="OO332_001137"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5941695.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19118..20023
                     /gene="yijE"
                     /locus_tag="OO332_001138"
     CDS             19118..20023
                     /gene="yijE"
                     /locus_tag="OO332_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5941696.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20050..20667)
                     /locus_tag="OO332_001139"
     CDS             complement(20050..20667)
                     /locus_tag="OO332_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5941697.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20942..22034)
                     /gene="gldA"
                     /locus_tag="OO332_001140"
                     /pseudo
     CDS             complement(20942..22034)
                     /gene="gldA"
                     /locus_tag="OO332_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22045..22707)
                     /gene="fsa"
                     /locus_tag="OO332_001141"
     CDS             complement(22045..22707)
                     /gene="fsa"
                     /locus_tag="OO332_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5941698.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22719..25220)
                     /gene="ptsP"
                     /locus_tag="OO332_001142"
     CDS             complement(22719..25220)
                     /gene="ptsP"
                     /locus_tag="OO332_001142"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5941699.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25529..26608
                     /locus_tag="OO332_001143"
     CDS             25529..26608
                     /locus_tag="OO332_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5941700.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26623..26943
                     /locus_tag="OO332_001144"
     CDS             26623..26943
                     /locus_tag="OO332_001144"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5941701.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            26994..29291
                     /locus_tag="OO332_001145"
     CDS             26994..29291
                     /locus_tag="OO332_001145"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5941702.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29257..30135
                     /locus_tag="OO332_001146"
     CDS             29257..30135
                     /locus_tag="OO332_001146"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5941703.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30137..30478
                     /locus_tag="OO332_001147"
     CDS             30137..30478
                     /locus_tag="OO332_001147"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5941704.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30465..31316)
                     /locus_tag="OO332_001148"
     CDS             complement(30465..31316)
                     /locus_tag="OO332_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5941705.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31465..33198)
                     /locus_tag="OO332_001149"
     CDS             complement(31465..33198)
                     /locus_tag="OO332_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5941706.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33381..36032)
                     /gene="ppc"
                     /locus_tag="OO332_001150"
     CDS             complement(33381..36032)
                     /gene="ppc"
                     /locus_tag="OO332_001150"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5941707.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36384..37535)
                     /gene="argE"
                     /locus_tag="OO332_001151"
     CDS             complement(36384..37535)
                     /gene="argE"
                     /locus_tag="OO332_001151"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5941708.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37689..38693
                     /gene="argC"
                     /locus_tag="OO332_001152"
     CDS             37689..38693
                     /gene="argC"
                     /locus_tag="OO332_001152"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5941709.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38701..39477
                     /gene="argB"
                     /locus_tag="OO332_001153"
     CDS             38701..39477
                     /gene="argB"
                     /locus_tag="OO332_001153"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5941710.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39538..40911
                     /gene="argH"
                     /locus_tag="OO332_001154"
     CDS             39538..40911
                     /gene="argH"
                     /locus_tag="OO332_001154"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5941711.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9337 a        10526 c        11255 g         9895 t
ORIGIN      
        1 agctgcaatt tgatcggaac tgatatcggc tttcgccagc acttctacca gcgtggagct
       61 ttgggtggcc cagatttcca ttgggtcgtg ttctacccaa cctggttttg ggtagatttg
      121 ctcaaattcg cgctgcgaca cgctaatgat attggcatcg tgatccatta cgaccgcgcg
      181 ggagctggtg gtgccctggt cgagcgcaac gatatatttt tttcagtcat gtttaattgt
      241 cccgtagtca tattacagcg aagctttttg ttctgaagga gttgtggttt ccttttcttc
      301 cacaacacag atatcgcaag gcaaatggcg accaatcagt ttgcggtagg caaatgcacc
      361 tacaatcgcg ccaacgatag ggccgaaaag cggcaccagg aagtaaggaa tgtctctgcc
      421 gccggtaaag gcgacattgc cccagcccgc cagctaggca aagactttcg gaccgaagtc
      481 acgcgctggg ttcatggcaa aacctgtcaa tgggcccata gatgcgccaa tgaccgcaat
      541 cagtagacca atcagcaagg gagccaaagg gccgcgtggt acaccgttgc catcgtccgt
      601 taacgccagg ataagcccca tcagaatagc ggtaatcacc atctcaactg cgaaagcctg
      661 cacaaaattg atatgaggat tagggtaagt agagaaagtg ccagccagat caacactttc
      721 aacgctgccg cgaacaatgt gatgagtctg ctcgaagtcg aaaaataaat tgtagtaaag
      781 cccgtaaact aaagccgcag cgcagaaagc gccggcaact tgtgaaacga taaaaggaat
      841 aactttgcgc ttgtcgaaac aggcaaacag ccacaatgca atggtaacag cgggattaag
      901 atgcgcgccg gaaacccctg cggtcaggta gatggccatt gccaccccca gtccccaaat
      961 gacactgatt tcccactgac caaaagacgc accagcgact tttagtgctg caacgcaacc
     1021 cacaccgaaa aaaatcaaca acccggtacc gaggaattca gcaatgcact ggcctttcaa
     1081 ggttgatgtt tgactcataa tcggatcctg aagagttaat gtttgttgta tgtgtgaaag
     1141 tcacggacct ccacgatgct tgtaggcatg ctgttaactt atcgttaacg agcaaaaacg
     1201 agaaatatcg aacttaaaat gtgtgtgctt cgtcataaaa tgagcgttat cgcgccattt
     1261 attccatctg attgtacatg atgcatgttt gttagatcat tcgcgcaaca aatttattaa
     1321 cattttcgat tgatgtggat tatgtcacta ggagaaggat gaaaagtgtg gcaaaccgta
     1381 atctgcaagg cgtgccgctg gacagcgatg gcgcggcttc atacaatcgg agctaactaa
     1441 agtgcgctcg tatttattaa ggcgtcaccg gtaatcggga cgaggatttt ttatccatca
     1501 acgccttgca attcaggaga ggtatgacaa tgtcattaga agtgtttgag aaactggaag
     1561 caaaagtaca gcaggcgatt gataccatca ctctgttgca gatggaaatc gaagagctga
     1621 aagaaaaaaa caactcactg tcgcaggaag ttcaaaatgc ccagcatcag cgcgaagagc
     1681 tggagcgtga gaacaaccat ctgaaagaac agcagaacgg ctggcaggaa cgtctgcagg
     1741 ccctgctggg tcgcatggaa gaggtctgat tacgcttcat ctttcgcgta atttctttac
     1801 tgtgtaggcc aacaggtaac gcagaaaaaa ggcaccttgc ggtgcctttc ttatcattca
     1861 atatccagcg gatcttctga aagaataatc ccggtattgt cggcataaag atggtcgccg
     1921 gagaagaagg tgacaccgcc aaaattgacg cggacatcgc tttcgccaat gccttcgcca
     1981 gcggcaccaa ccggaattgc cgccatcgcc tggatgccga tatccaactc ttccaggtca
     2041 tctacctgac gcaccgcgcc gtaaatgacc agaccttccc attcattttg tactgccaga
     2101 cgcgccagtt cagcatcgac cagtgcgcga cgaacagaac caccgccatc gacgacaaga
     2161 acacgaccac ggccattctg ttcgagcaga tcgtacagca acccgttgtc ctcgaaacat
     2221 tttaccgtga ttatttgtcc gccaaacgac gcccgtccgc caaagttgga gaacagcggt
     2281 tccacgacgt taacatcttc ttgatagatg tcacaaagct cggaagtatc gtatttcata
     2341 ggcttaacat tcagttgctg cgagaatttt cagtatatcg cgctatgtgg gctgttggca
     2401 aagtcatcaa ttgttaattg atatttgtca gttatgctgc ccactggctt aggaatatcc
     2461 ctaaaacaaa cagcaggtta gtcagtaacg ctcccttgac agtacgttcc agcattggtc
     2521 gcatcgccac cgggtccatt tcccgcatca cataacgggc ttgcttcacc agtaatggtg
     2581 ccgccagcag gaacagccag ccccacaggc tatgcagcga aaagagatta aacagcgcca
     2641 gacacaccag cgagcccatc agcaggcagg catgataacg acgcgcgttc acttcaccta
     2701 agcgcaccac cagcgtgttt ttgccatttt cgcggtcgct attgatatca cgcaggttat
     2761 taatattcag tactgccgtt gccagcaggc cgcatgcggt cgccggaagg atcagtgccg
     2821 gaatcaatgt atgagcctgt aaataccagc tccccatgac actcaaccag ccaaagaaaa
     2881 ccagtacgga aatatcaccc agaccgatat aaccataagg acgattgccc acggtgtagg
     2941 tgatagcggc aatgatcgac aacccgccaa gaatcaggaa accgacaaaa tcggccagcg
     3001 tatggcatgc cactgcaacc agtgccagcc cggagagaca gatgagcacg acggtaataa
     3061 tgagcgcccg tttcatcttt tgctgggtaa tgaccccttt ttgcatgccg cgtagcggcc
     3121 caatgcggtc aggtttatcg ctgcctttta ccgcatcgcc gtaatcattg gcgaggttag
     3181 aaaggatctg taatagcccg gcggtaatta gtgccagcag ggcgaccagc ggatcgaagt
     3241 gaccttgcca ccatgccagc gccgtcccga cgataattgc agcaaaggcg agggggaggg
     3301 ttttaggtcg taaactttcc agccacgcct gagttcggct aatttgttgt tcagtcataa
     3361 tacgcgccaa taaaaatggg gcctttcagc cccatcaaac aatgatgaaa atgattgaac
     3421 gcgattatag gataaaacgg ctcagatctt catctgccac caacgcatcc agatgtttgc
     3481 tcacataatc tgcgtcaata gtgatatttt gaccgcttaa atcgctggcg tcgtaggaaa
     3541 tctcttccat taaacgctcc agaacagtgt gtaaacgacg agcaccgatg ttttcggtag
     3601 attcgttcac ctgccatgcc gcttccgcga tgcgtttaat accggagtcg gtaaactcga
     3661 tatttacgcc ttcagtcgcc atcagtgctt tgtactgcac ggtgatagag gcattcggct
     3721 cggtcagaat acgctcgaag tcgctggtgg tcagtgcctg cagttcaacg cggattggca
     3781 gccgaccttg cagttccggg atcaggtcag acggtttcgc aatctggaac gcgccagaag
     3841 cgataaacag aatgtggtca gttttgacca tcccgtgttt ggtggaaacg gtgcaacctt
     3901 ctaccagcgg cagcaggtca cgctgaacgc cttcacgaga aacatccgga ccggaagact
     3961 cgccgcgctt acagattttg tcgatttcgt cgataaacac gatcccgtgc tgctcaacag
     4021 cgtcgatagc gtcttgcttc agctcttccg ggttcaccag tttcgccgct tcttcttcaa
     4081 tcagcagctt catggcgtct ttgattttca gcttacgcgc tttttgcttc tggccgccca
     4141 ggttctggaa catggactgc agctggctgg tcatctcttc catgcccgga ggagccataa
     4201 tttcaacgcc catcggtgct gcggcaagat cgatctcgat ttctttgtca tcaagctggc
     4261 cttcacgcag ttttttgcgg aatgcctgac gagcagcgga aggttcctgc tgctgttcag
     4321 tctgtcccca gttgttttta gcaggtggga tcagcacgtc gagaatacgt tcttctgcca
     4381 gttcttcagc gcgataacgg tttttctcga tagcctggac gcgtaccatt ttcacggcgg
     4441 catcggtcag atcgcgaata atagaatcaa cttccttacc gacgtagccc acttcggtaa
     4501 atttggtcgc ttcaactttg atgaacggcg cattcgccag cttagccaga cgacgggcga
     4561 tttcagtttt accgacaccg gtcgggccga tcatcaggat atttttcggg gtcacttcat
     4621 ggcgcagctc ttcgttgagc tgcatgcgac gccagcggtt acgcagagca atcgccacag
     4681 aacgcttggc gttgtcctgg ccgatgatgt gcttatccag ttcgctgacg atttcgcgtg
     4741 gggtcatttc agacatggga gatccttacg ctttgtagct taattcttcg atggtgtgga
     4801 aatggttggt atagatgcaa atgtcgcctg caatatccaa cgccttttca gcaatttcac
     4861 gggcgctaag ttcagtgttt tctaacagcg cgcgcgccgc agcctgggcg taagggccgc
     4921 cggagccgat agcaataaga tcgttttctg gctgcaccac gtcaccgtta ccggtgatga
     4981 taagcgatgc agtttcatcc gcgactgcca gcagtgcttc aagtttgcgc agcatgcgat
     5041 cggtacgcca gtcttttgcc agctcaacgg cggctttaac cagatggccc tgatgcattt
     5101 ccagtttacg ttcaaacagt tcgaacagcg taaaagcatc cgcagtaccg cccgcaaagc
     5161 ccgcgatgac tttgtcgttg tacagacggc ggaccttttt cacgttgcct ttcattacgg
     5221 tattgcccaa cgtggcctga ccatcaccag cgatgaccac atggccgtta cggcgtacgc
     5281 ttactatagt tgtcacgagc tgaccccttg gttacgaata cagagtacaa accccgtaca
     5341 aaagtacggg gcataatgca attatagatg ggggggattt tgagggtttc aacccccggc
     5401 ggcgagccga atgcagtttg tatgacccgc catcttcaac cgattgaggg tgctgtctgc
     5461 gttctctttg cctttcaccg ggccaatgac cacacgattc cagccattgt tggtggtgat
     5521 tttcgagtca aatccttcga acgccagctg agcacgtacc gtctctgcct gttccgcgcc
     5581 tctgaacgaa ccgcactgca ccatccagcg gcgttcgtct tttttctccg ccgtcggttt
     5641 tggcgcgtca gcggcacgag caaccggcgc agcttgctgc ggcttcgact gcgcggtcgt
     5701 gtgcgcaggg gtttgcagca gatcctggta cggctgctga gtagaagctg gtttagactg
     5761 gcgcggttgc gcctgtaccg gcgcggcttg cgacgtacgc gtctgctgct gccagctttg
     5821 ttcagtcgta cgcgactgct gagccaggcg ttgctgctcc gctaattgct gcgcctgacg
     5881 ctggcgttgt agcgtttgct gacgctgttc cggcgtctgt tcattccacg gaacctcaac
     5941 cagctgcgtt ggctgctggc gcatatcagc ctgcatttgt tcaagaagct gacgttgttc
     6001 tggtgtcagt tgctccggcg ttttcacttc accaccggca gaaggttctg tgggcgcacg
     6061 cacacccggc tggcgacttt ccagctcttt aatgtagcgc cagcgttctt ctggttttgg
     6121 tggtagtccg tttccggtca ctttctggct ttgcagcgtc tcggactctt ctttcttgtg
     6181 atgcgtaatg aagtacagac caccgataaa ggtcacaaga acggcggcag caatagcgac
     6241 catagcggga gaaaccgcag gcagatttcg ttgctttttc cgtgaggtgc tcttttttcg
     6301 ccgcgaaggt gccggttggc tgcggcgtac ataatctcgt tgtgccacta tcgtttcgct
     6361 gtatttattc gttcgtcagc ccgccatgtt acttaagcgg cgggcctttg accagtcacg
     6421 gcagtcttaa aggtttactt taaggtaacg cgcgtgttga tccccggatg ataagttcgc
     6481 agtccattaa acgagagcca ctgccaacgt gttgcccctg catttgatca agcaataaca
     6541 gcatagcttc ccgaccgatt tcgtaacgcg gctgcgcgat ggttgtcagc ggcggatcac
     6601 aaaattgcgt caggtcgatg ttatcaaaac cgattatgga aaggtcttcc gggactttca
     6661 gcccctggcg ttttgcctga gaaagtgcgc cgagcgccat cacatcgcta tggcagaaga
     6721 ctgcagtagg cggttgtgga agatcaagca gctgttgcat tgctttgctt ccggcttcga
     6781 aggtgaagtc gccacgggcg atgtattgtg gatcaaccat aattccgcag cgacgcagcg
     6841 cctgaacata gccttgcagg cggtagtgac acagtggcat ctcttcggga ccggctatac
     6901 agccaatccg tttatgccct tgctcatata aataatttac tgcatcaaat gcggcggtca
     6961 gattgtcgat atgaacggta ggcagctcca gttccggtgc aaattcgttc gccatcacca
     7021 tcggcggcag attacgctgt tcctcaatgc tggcatcaaa cggcagcctt gaacccagca
     7081 gcaacatgcc atcaatttgc ttggtgatga tcaaatcgat aaaggttttt tcctgctgat
     7141 tttgatgcgc acagtcgcca atcagcacca gatatccgtg atttgccgcc gtaacttcga
     7201 taccgcgaat aatttcgcta aagaagggat cgcagatatc cgggacaatc accagaatgg
     7261 tgcgggattc attacgcttg acgttgcgcc ccataggctg cggtaaataa cccacttccc
     7321 gggccgcttt ttcaacccga ttacgggtgg cctgggagac tttatcggga ttcattaatg
     7381 ctcgggagac agtcgctgta gagacttttg ccttgagggc aacgtctttc atggtcgcgg
     7441 cagtttcctg cttcttcgct ttcacactca ctcctcgcct ggcacgtcag gcgtactaca
     7501 tccatgttta cttcacatcg gcaacatttt tagcagatag cgcgtgaaaa cggttacaga
     7561 attttcatga aaagtgtgat gaatattgaa tttttcgatc cgcctcgcat cgtaagcggt
     7621 ttaaccctca atcggatcaa catccagtac ccatttcacc ttgcgggaat ccggtattgt
     7681 attgatgagc gccagcgtac cactaatgat gtgttgcaag cgcacgcggg aagggtgctg
     7741 caacaatatc tgccagcgcc agcggccgcc acgtttaggt gccagtgccg gaaccggacc
     7801 gagaacccac agtttgtcgt ctgccagcgg actggagagg atcagattac gcagttgttg
     7861 caggaacagc ggcgcatgct gattgttatg atcttccgca cgcacaatca catggctggt
     7921 ccatggcggc agttgcatca tccgtcgctc agccagcgcc tgttcggcaa aggcgtcgta
     7981 gcctttatag agcaacgttt gcaacagagg atgttccgga tggtgcgttt gcagcaccac
     8041 ttcgccttgt ttacccgcac gcccggcacg accagcgacc tgggtgtaaa gctgagcgaa
     8101 acgctctgcc gagcgaaaat cggcagaaaa cagcgcgccg tccacatcca gtaacgccac
     8161 cagcgtcaca tccggaaagt ggtgaccttt cgccagcatt tgtgtgccga tcaaaatcct
     8221 cgcaccgccg cgatgtactt ccgccagttg ctgttccagc gcccctttgc ggctggtggt
     8281 atcgcggtcg atacgagaaa tgggcacatc agggaacagc ggcgcaagcg tctgttcaag
     8341 ctgttcggtg cccagcccca cgggtaccag gtgcgtggaa ccgcaggaag ggcactggcg
     8401 cggcaccgga cgctgactgt cacagtggtg acagcgcaaa tgctgttgcg cctgatgcag
     8461 cgtgtagtag tgatcgcaac gtgggcattc ggcaatccag ccgcagtcgt ggcacagtag
     8521 cgcaggcgca aagccacggc ggttaagaaa gaggataacc tgattgttgg cctgtaaatg
     8581 ctggcgcata cgggtgatta acgccggagc cagacctgcc tgcaccttct gaccttttaa
     8641 atccagcacg tgttgaattg ccggacgcgc attccccgcc cggcgcgtca ggcgcagcag
     8701 gcggtatttt ttctgctgga cgttgcacaa cgtttccagc gcgggcgttg cggagccaag
     8761 aataatcggg atttgctcgc tatgcgcacg atacaccgcc aggtcgcggg catgatagcg
     8821 ccagccttcc tgctgcttgt aggaactgtc gtgctcttcg tcaatgacaa tcacgccgag
     8881 atttttaaac ggcgtaaaca gcgcggagcg ggtgccgatc acaatcgccg cctcgccatt
     8941 tttcgctttc agccacgccg aaagacgctc gctgtcgttc agaccggaat gcagaacttc
     9001 cacgggcgca ttaaaccgtt cacgaaaacg ggcgatagtt tgcggtgtca ggccgatttc
     9061 cggcaccatc accagcgcct gtttgccctg agcgagcacg ttttccagta cactgagata
     9121 aacctccgtt ttaccggaac cggtaacgcc cgccagcagc caggcagaaa aagtatctgc
     9181 cgcgctatga attgcgccaa cggcggtggc ctgttcggta ttcaatcgca accgctcacc
     9241 agaaacggca tagttcgttc gccagtcgct aaactctggt gtttcacttg ctaaatcaca
     9301 cagacctttt ttgcgcagcg cctgcaacgc ggcatcatta aattcgagcg tggcaacctg
     9361 gtcgcgccag attttgcctt gccgtaacgc tgccagcgcc tgttgttgct tgggtgagcg
     9421 tttcaggctg ttcagatcca ccgcctgacc ttgttcagtg gcaaaccagt accacatcgg
     9481 cgcgttcgcc gcaggccgcc cctggcgtag taaaatcggc aaggcatgaa acagcacatc
     9541 gccaatcgga tgatgatagt aatctgccgc ccatagcagc aatcgccaga cggagtgagt
     9601 aaacaccggc tcaccatcca gcacttcgac taccgctttt agctcagtga gcggcagttc
     9661 gctggcatcg ctaactgata ccacaatccc gatgcgctcc tgctgtttgc caaacggcac
     9721 gcgcacgcga cacccagctt taaccgtcat gccttctggc agcagatagt caaaggtacg
     9781 aggaagcgga acgggcaagg caacgtgggc aacgggcata gcatcatcct gacttgaaat
     9841 tcggtgggtt agtatacaca ttgccgtaga agagtgcgga tcagtttgca tacgctggtt
     9901 aatttctgta tgatttcgcg ccttcgtacg aaatgatcgt attgaagcta tacttttaac
     9961 atcgcgtggt gtctggcgtt agggctggaa gagcgacgcg gccttaaacc gaggttttcc
    10021 catgaaaaaa gatattcacc cgaaatacga agaaattact gctagctgct cttgcggtaa
    10081 cgtaatgaaa atccgctcca ccgttggtca tgacctgaac ctcgacgtgt gcagcaagtg
    10141 ccacccgttc ttcactggca aacagcgtga tgttgctacc ggtggccgtg ttgaccgctt
    10201 caacaagcgt ttcaacatcc cgggcagcaa ataagaccgg atttccgaaa aaagcgccgc
    10261 acggcgcttt ttttgtgcct gaattttggt cgtatcacca cgaaaacagc ggccactctt
    10321 tggcaaccgt ggtgagttgt ggcgcgtcga aaacggcttg tggtgaaact accgtttcct
    10381 caagcggaat atttttaccg tagcgttctt tgagcttcgc ctggaccagc gggttgctga
    10441 gatcaaactc tttcaacttc tgctgttcgc ccacgcgcat tcccagcgcg ttctggtaaa
    10501 ccttccacac cacttccgaa cagtactggc gatcgtcgct ccatgagaag ctgaaatcat
    10561 acggtttacc gagataacgt tttgccgttt gcgtcagttt ttgctgttgt tcaacactca
    10621 gtccgccttc aacgcggcga acaacgtagt tgcccttttc accatgggca atccactgct
    10681 tgagcggggt gtatttcacc gggccgaccg cttcaaaaac gtagggcttt ttgttacgca
    10741 tcaccagcat accggtgtgg ctgtaatcgg aatgggttgc cagttggatc gctttacttt
    10801 gcgatgagcg agagatttga aagatgatgt cgccagtttg cggctgccag gcaaaggcag
    10861 gtacagaaac cagcaggctg aggatcagca gcctgttttt catagttaaa cgtccatgta
    10921 taaaaagcgg tgggtcgcag acaacgtgct cgttgtttat gccggatgcg gcgtgaacgc
    10981 cttatccggc ctacaagttc gtgcaaattc aataaattgc aatatgacgt aggcctgata
    11041 agcgtagtgc atcaggcgat tccactccgc gccgctcttt tccgctttag tattcccacg
    11101 tctccgggtt aatccccatc tcacgcatga tctcttttgc cgcttccggg atttcgtcgc
    11161 tgcgctcttt acgcagatcg gcatcatccg gcaaaggttg cccggtaaag gcatgcagaa
    11221 acgcttcgca cagcagctcg ctgttggtag cgtgacgcag gttgttcacc tgacgacgcg
    11281 tgcgttcatc ggtgaggatt tttaacacct taagaggaat ggaaaccgta atctttttga
    11341 cttgttcact cttcttgccg tgttcagcgt atgggctgat atattcgccg ctccattcag
    11401 ccatgagata cttaatcctc ttcgtcaata aattgagacc agaccccaca atttatgtgg
    11461 gtactgaccg taaacccgca tagtttaccg tacaggcgtt accgtgacat cgtgtaatgc
    11521 acctgtcggc gtgataatgc atataatttt aacggctatt tgggatttgc tcaatctata
    11581 cgcaaagaag tttagatgtc cagatgtatt gacgtccatt aacacaatgt ttactctggt
    11641 gcctgacatt tcaccgacaa agcccaggaa acttcatcac atgacgcgta aacaggccac
    11701 catcgcagtg cgtagcgggt taaatgacga cgaacagtat ggttgcgttg tccctcccat
    11761 ccatctttcc agcacctata actttaccgg atttaatgaa ccgcgcgcgc atgattactc
    11821 gcgtcgcggc aacccaacgc gcgatgtggt tcagcgtgcg ctggcagaac tggaaggcgg
    11881 tgctggcgca gtacttacta ataccggcat gtccgcgatt cacctggtaa cgaccgtctt
    11941 tttgaaacct ggcgatctgc tggttgcgcc gcacgactgc tacggcggta gctatcgcct
    12001 gttcgacagt ctggcgaaac gcggttgcta tcgcgtgttg tttgttgatc aaggcgatga
    12061 acaggcatta cgggcagcgc tggcagaaaa acccaaactg gtactggtag aaagcccaag
    12121 taatccattg ttacgcgtcg tggatattgc gaaaatctgc catctggcaa gggaagtcgg
    12181 ggcggtgagc gtggtggata acaccttctt aagcccggca ttacaaaatc cgctggcatt
    12241 gggtgccgat ctggtgttgc attcatgtac gaaatatctg aacggtcact ctgacgtggt
    12301 ggctggcgtg gtgattgcta aagacccgga cgttgtcact gaacttgcct ggtgggcaaa
    12361 caatattggc gtgacgggcg gcgcgtttga cagctatctg ctgctacgcg ggttgcgaac
    12421 gctggtgccg cgtatggagc tggcgcagcg caacgcgcag gcgattgtga aatatctgca
    12481 aacccagccg ttggtgaaaa aactgtatca cccgtcgctg ccggaaaatc aggggcatga
    12541 aattgccgcg cgtcagcaaa aaggctttgg cgcaatgttg agttttgaac tggatggcga
    12601 tgagcagacg ctgtgtcgtt tcctgggcgg gctgtcgttg tttacgctgg cggaatcatt
    12661 agggggagtg gaaagtttaa tctctcacgc cgcaaccatg acacatgcag gcatggcacc
    12721 agaagcgcgt gctgccgccg ggatctccga gacgctgctg cgtatctcca ccggtattga
    12781 agatggcgaa gatttaattg ccgacctgga aaatggcttc cgggctgcaa acaaggggta
    12841 aaaatgagtg tgattgcgca ggcaggggcg aaaggtcgtc agctgcataa atttggtggc
    12901 agtagtctgg ctgatgtgaa gtgttatttg cgtgtcgcgg gcattatggc ggagttctct
    12961 cagcctgacg atatgatggt ggtttccgcc gccggtagca ccactaacca gttgattaac
    13021 tggttgaaac taagccagac cgatcgtctc tctgcgcatc aggttcaaca aacgctgcgt
    13081 cgctatcagt gcgatctgat tagcggtctg ctacccgctg aagaagccga tagcctcatt
    13141 agcgcttttg tcagcgatct tgagcgcctg gcggcgttgc tcgacagtgg tattaacgat
    13201 gcggtgtatg cggaagtggt gggccacggt gaagtgtggt cggcgcgcct gatgtctgcg
    13261 gtacttaatc aacaagggct gccagcggcc tggcttgatg cccgtgagtt tttacgcgct
    13321 gaacgcgctg cgcaaccgca ggttgatgaa gggctttctt acccgttgct gcaacagcta
    13381 ctggtacaac atccgagcaa acgtctggtg gtgaccgggt ttatcagccg taacaacgcc
    13441 ggtgaaacgg tgttgctggg gcgtaacggt tccgactatt ccgcgacaca aatcggtgcg
    13501 ctggcgggtg tttctcgtgt aaccatctgg agcgacgtcg ccggggtata cagtgccgac
    13561 ccgcgtaaag tgaaagatgc ctgcctgctg ccgttgctgc gtctggatga ggccagcgaa
    13621 ctggcgcgtc tggcggctcc cgttcttcac gcccgtactt tacagccagt ttctggcagc
    13681 gaaatcgacc tgcagctgcg ctgtagctac acgccggatc aaggttccac gcgcattgaa
    13741 cgcgtgctgg cctccggtac tggtgcgcgt attgtcacca gccacgatga tgtctgtttg
    13801 attgagtttc aggtgcccgc cagtcaggat ttcaaactgg cgcataaaga gatcgaccag
    13861 atcctgaaac gtgcccaggt gcgtccactg gcggttggcg tgcataacga tcgccagttg
    13921 ctgcaatttt gctacacctc agaagtggcc gacagtgcgc tgaaaatcct cgacgaagcg
    13981 ggattacctg gagaactgcg cctgcgtcag gggctggcgc tggtggcgat ggtcggggcg
    14041 ggtgtcaccc gtaacccgct gcattgccac cgcttctggc agcaactgaa aggccagccg
    14101 gtcgagttta cgtggcagtc cgatgacggc atcagtctgg tggcagtact gcgcaccggc
    14161 ccgaccgaaa gcctgattca ggggctgcat cagtccgtct tccgcgcaga aaaacgcatc
    14221 ggcctggtat tgttcggcaa aggcaatatc ggctctcgct ggctggaact gttcgcccgc
    14281 gagcagagca cgctttcggc gcgtactggc tttgaatttg tgctggcagg cgtggtggac
    14341 agccgccgca gtctgttgag ttatgacggg ctggacgcca gccgcgcgtt agccttcttc
    14401 aacgatgaag cggtcgagca ggatgaagag tcgcttttcc tgtggatgcg cgcccatccg
    14461 tatgatgatt tagtggtgtt ggacgttacc gccagccagc agcttgctga tcagtatctc
    14521 gatttcgcca gccacggttt ccacgtcatc agtgccaaca aactggcggg agcaagcgac
    14581 agcaataaat atcgccagat ccacgacgcc ttcgaaaaaa ccgggcgtca ctggctatac
    14641 aacgccaccg ttggtgcggg cttgccgatc aaccataccg tgcgcgatct gatcgacagc
    14701 ggcgatacca ttttgtcgat cagtgggatc ttctccggca cgctctcctg gctgttcctg
    14761 caattcgacg gtagcgtgcc gtttaccgag ctggtggatc aggcgtggca gcagggctta
    14821 accgagcctg atccgcgtga cgacctctcc ggcaaagacg taatgcgcaa gctggtgatt
    14881 ctggcgcgtg aagcgggtta caacattgaa ccggaccagg tacgtgtgga atcgctggtg
    14941 cctgctcatt gcgaaggtgg cagcatcgac catttctttg aaaatggcga tgaacttaac
    15001 gagcagatgg tgcaacggct ggaagcggcc cgcgaaatgg ggctggtgct gcgctacgtg
    15061 gcgcgtttcg atgccaacgg caaagcgcgt gtgggcgtgg aggcggtgcg tgaagaccat
    15121 ccgctggcat cactgctgcc atgcgataac gtctttgcca tcgaaagccg ctggtaccgc
    15181 gataatccgc tggtgatccg tgggcctggc gctgggcgcg acgtcaccgc cggggcgatt
    15241 cagtcggata tcaaccggct ggcacagttg ttgtaatttc agaaatttaa taatgcccgg
    15301 tactcatgtt ttcgggtcta tggtttctaa tgaaatatat tgaattatca tagaattagg
    15361 ccggatgaag cgtttacaac gaatccggca agaagcaata agtacatggt tagtttatat
    15421 ttgcagtccg gtttgctttg catatcggat tttctttttc ttaccatcct gaagtttttt
    15481 catcttccct gatttttcct caccatcatt ggtcattttt cggttgacgc ccttcggctt
    15541 ttccttcatc tttacatctg gacgtctaaa tggatagatg tgcacaacac aacatataac
    15601 tacaagcgat tgatgaggta aggtatgagc ttttttcacg ccagccagcg ggatgccctg
    15661 aatcagagcc tggcagaagt ccaggggcag attaacgttt cgttcgagtt tttcccgccg
    15721 cgtaccagtg aaatggagca gaccctgtgg aactccatcg atcgccttag cagcctgaaa
    15781 ccgaagtttg tatcggtgac ctatggcgcg aactccggcg agcgcgaccg tacgcacagc
    15841 attattaaag gcattaaaga tcgcactggt ctggaagcgg caccgcatct tacttgcatt
    15901 gatgcgacgc ccgacgagct gcgcaccatt gcacgcgact actggaataa cggtatttgt
    15961 catatcgtgg cactgcgtgg cgatctgccg ccgggaagtg gtaagccaga aatgtatgct
    16021 tctgacctgg tgacgctgtt aaaagaagtg gcagatttcg atatatccgt ggcggcgtat
    16081 ccggaagttc acccggaagc aaaaagcgct caggcggatt tgcttaatct gaaacgcaaa
    16141 gtggatgccg gagccaaccg cgcgattact cagttcttct tcgatgtcga aagctacctg
    16201 cgttttcgtg accgctgtgt atcggcgggc attgatgtgg aaatcatccc gggcattttg
    16261 ccggtatcta actttaaaca ggcgaagaaa tttgccgaca tgaccaacgt gcgtattccg
    16321 gcgtggatgg cgcaaatgtt tgacgggctg gatgacgatg ccgaaacccg caaactggtg
    16381 ggcgcgaata ttgccatgga tatggtgaag attttaagcc gtgaaggagt gaaagatttc
    16441 cacttctata cgcttaaccg cgctgaaatg agttacgcga tttgccatac gctgggtgtt
    16501 cgacctggtt tataaatagt gtggcttttg tgaaaatcac acagtgatca caaattttaa
    16561 acagagcaca aaatgctgcc tcgaaatgag ggcgggaaaa taaggttgtc agccttgttt
    16621 tctccctcat tacttgaagg atatgaagct aaaacccttt tttataaagc atttgtccga
    16681 attcggacat aatcaaaaaa gcttaattac gatcaatttg atctacatct ctttaaccaa
    16741 caatatgtaa gatctcaact atcgcatccg tggattaatt caattataac ttctctctaa
    16801 cgctgtgtat cgtaacggta acactgtaga ggggagcaca ttgatgagca cgtcagacga
    16861 tatccataac accacagcca ctggcaaatg cccgttccat cagggcggtc acgaccagag
    16921 cgcgggggcg ggcacaacca ctcgcgactg gtggccaaat caactccgtg ttgacctgtt
    16981 aaaccaacat tctaatcgtt ctaacccgct gggtgaggac tttgactacc gcaaagaatt
    17041 cagcaaatta gattactacg gcctgaaaaa agatctgaaa gccctgctga cagaatctca
    17101 gccgtggtgg ccagccgact ggggcagcta cgccggtctg tttattcgta tggcttggca
    17161 cggcgcaggt acttaccgtt caatcgatgg acgcggcggc gcgggtcgtg gtcagcaacg
    17221 ttttgcaccg ctgaactcct ggccggataa cgtaagcctc gataaagcgc gtcgcctgtt
    17281 gtggccaatc aaacagaaat atggtcagaa aatctcctgg gccgacctgt ttatcctcgc
    17341 aggtaacgtg gcgctggaaa actccggctt ccgtaccttc ggttttggtg ccggtcgtga
    17401 agacgtctgg gaaccggatc tggatgttaa ctggggtgat gaaaaagcct ggctgactca
    17461 ccgtcatccg gaagcgctgg cgaaagcacc gctgggtgca accgagatgg gcctgattta
    17521 cgttaacccg gaaggcccgg atcacagcgg cgaaccgctt tctgcggcag cagctatccg
    17581 cgcgaccttc ggcaacatgg gcatgaacga cgaagaaacc gtggcgctga ttgcgggtgg
    17641 tcatacgctg ggtaaaaccc acggtgccgg tccgacatca aacgtaggtc ctgatccaga
    17701 agctgcaccg attgaagaac aaggtttagg ctgggcgagc acttacggca gcggcgttgg
    17761 cgcagatgcc attacctctg gtctggaagt agtctggacc cagacgccga cccagtggag
    17821 caactatttc ttcgagaatc tgttcaagta tgagtgggta cagacccgca gtccggctgg
    17881 agcaatccag ttcgaagcgg tagacgcacc ggaaattatc ccggatccgt tcgatccgtc
    17941 gaagaaacgt aaaccgacaa tgctggtgac cgacctgacg ctgcgttttg atcctgagtt
    18001 cgagaagatc tctcgtcgtt tcctcaacga tccgcaggcg ttcaacgaag cctttgcccg
    18061 tgcctggttc aaactgacgc acagggatat ggggccgaaa tctcgctaca tcgggccgga
    18121 agtgccgaaa gaagatctga tctggcaaga tccgctgccg cagccgatct acaacccgac
    18181 cgagcaggac attatcgatc tgaaattcgc gattgcggat tctggtctgt ctgttagtga
    18241 gctggtatcg gtggcctggg catctgcttc taccttccgt ggtggcgaca aacgcggtgg
    18301 tgtcaacggt gcgcgtctgg cattaatgcc gcagcgcgac tgggatgtga acgccgcagc
    18361 cgttcgtgct ctgcttgttc tggagaaaat ccagaaagag tctggtaaag cctcgctggc
    18421 ggatatcatc gtgctggctg gtgtggttgg tgttgagaaa gccgcaagcg ccgcaggttt
    18481 gagcattcat gtaccgtttg cgccgggtcg cgttgatgcg cgtcaggatc agactgacat
    18541 tgagatgttt gagctgctgg agccaattgc tgacggtttc cgtaactatc gcgctcgtct
    18601 ggacgtttcc accaccgagt cactgctgat cgacaaagca cagcaactga cgctgaccgc
    18661 gccggaaatg actgcgctgg tgggcgggat gcgtgtactg ggtgccaact tcgatggcag
    18721 caaaaacggc gtcttcactg accgcgttgg cgtattgagc aatgacttct tcgtgaactt
    18781 gctggatatg cgttacgagt ggaaagcgac tgacgaatcg aaagagctgt tcgaaggacg
    18841 tgaccgcgaa accggcgaag tgaaatacac agccagccgt gcggatctgg tatttggttc
    18901 taactctgtc ctgcgtgcag tagcggaagt ttacgccagc agcgatgccc acgagaagtt
    18961 tgttaaagac ttcgtagcgg catgggtgaa agtgatgaac ctcgaccgtt tcgacctgct
    19021 gtaatctgac cttcttcagc gactgtcttt caggcagtcg ctgaagtttc tttaccggcg
    19081 tatagtgtcc acaggaaaac tacacactgg atctctcatg tctgccgcag gaaagagcaa
    19141 cccactggca atcagtggcc tggttgtgct cacacttatc tggagttata gctggatttt
    19201 catgaagcaa gtcaccagtt acatcggtgc cttcgacttt accgccttac gctgcatttt
    19261 cggcgctctc gttttattca tcgtcctttt attacgtggt cgcggaatgc gcccgacgcc
    19321 gtttaaatac accttagcca ttgccctgtt acaaacctgc gggatggttg gtctggcgca
    19381 gtgggcgttg gtcagcggag gtgcggggaa ggtggcgatc ctgagctata ccatgccgtt
    19441 ctgggtggtg attttcgccg cgttgtttct cggtgaacgc ctgcgacgtg ggcaatattt
    19501 cgcgattctg attgccgctt tcggcttatt tttggtgttg cagccgtggc aactcgattt
    19561 ctcttcgatg aaaagtgcca tgctggcaat cctctccggt gttagttggg gggcgagcgc
    19621 gattgttgct aaacgcctat acgcccgtca tccgcgcgtg gatttattat cgttaacatc
    19681 ctggcagatg ctgtatgcgg cgctggtgat gagtgtggtc gctttactgg tgccgcaacg
    19741 tgaaattgac tggcagccca ccgtgttctg ggcgctggcc tacagtgcga ttctggcgac
    19801 ggcgctagcg tggagcttat ggctgtttgt gctgaaaaac ttgcctgcca gtattgccag
    19861 cttaagcaca ctggccgttc ccgtttgtgg cgtactcttt tcctggtggc tgctcggtga
    19921 gaatccgggg gccgttgaag gtagcggtat tgtgctgatt gtgctggcac tggcgctggt
    19981 gagccgtaag aaaaaagaag ctgtcagtgt aaaaagcatc tgaatttttt cttcatgtgg
    20041 ggcgatctct tatttaacaa aataacgata atgccccacc atccgccagc taaacagcac
    20101 atcttcttcc tgcgcgcctg cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc
    20161 gaagccatct gacgccaccc caatatgcgc cagcccgtta tctaatcgcc aggagacaat
    20221 atcgcccgct tgatagtcac cggcattttt actgatgggg cgtgttttgt tgtggcggct
    20281 aaaccaggtt tccagattag gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa
    20341 cttccatttt tgcgggtact cagcaaaatt cttcgccata tcttcgtgaa ccagtttctg
    20401 caaatcgacc ttctggctgc gcaatgcgcg gatcaccaca tcggaacata caccgcgttc
    20461 ttgcggaaca tcaccgccag gataggtaag ctgcatatac gccggatcgt aaaatagcgt
    20521 gctgccaatt tgctgtctgg caccgtctgc gatggcaagg ttggtattgg cctggatttg
    20581 taccacggtt ggtggaacgg cgggagattt taaggagtgg ctggtaaatg ccgttagcag
    20641 gctgagcagc gccagtgaag ctttcatctg gttcgtccgt gaaaataaga gtcacagatt
    20701 cgaccttccg ggctaacgat tgttctgtgg agaaaatgtc cagaagcgga taaaaaactc
    20761 ctgaacaacg tgggagtttc agattactgg cacagagtgc cggatgcgga gtgaacgcct
    20821 tatccggcct acaaaagcac gcaaattcaa tatattgcag agattgcgta ggcctgataa
    20881 gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag tttggagtag
    20941 gttattccca ctcttgcagg aaacgctgac cgtactggtc agctaccagc agtgcggcgt
    21001 aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacct tctgcacaag
    21061 atgcttttgc aacaatgcgc attttcgccg ggacatcttc tttaatatcc agttgagcga
    21121 gagttattgg caaacctacc gcatggctaa gtgcagctac ggtttcgatt tcctccaccg
    21181 gcgcgttttc cagtaccagc tgcgtcagcg taccaaatgc cactttttca ccgtgataat
    21241 agtgatgcgc gtccgggata gcggtcaggc cgttatgcac cgcgtgagcc gccgctttca
    21301 aaaccaacgc cgctcaaata ggtgttcgct tcaatcacgc gctccagcgc cggggttact
    21361 acatgctgtt cggcggcaag catcgctttt tcgccttctt ccagcagggt gttgtagcac
    21421 agttcagcca gtgccagcgc cgcctgggtg cacttgccgc ccgccatggt ggtcgcgccg
    21481 ctacgagagc aagcacgcgc ttcaaaccag gttgccagcg catcgccgat acccgctgct
    21541 aacagacgtg caggtgcgcc cgcgacgatt ttggtgtcga caatgaccat attcgggtta
    21601 tttggcaaca gcagatagcg gtcaaactca ccctcatcgg tgtagataac agacaatgcg
    21661 ctgcacggtg catcggtaga ggcgatagtc ggtgcgatcg ctaccggaac acccatgaaa
    21721 tgtgccagtg ctttggcagt atcgagggtt tttccgccac cgataccgag aattgcgcca
    21781 cactgcgcag tctccgcgat gccacgcaga cggtcgatct cattttgcga acattcaccg
    21841 ccaaacggcg taatttctac taccagtcca gcatctttaa agcttttctc gacagtggat
    21901 tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc agcgttctgc cagcggcttc
    21961 aggtattcgc ccagacgatt aatcacatca gcgccctgga tgtatttacc cggtgattga
    22021 ataatgcggt ccataattgc tcctttagag atgagtagtg ccaaatgcgg cattccagtc
    22081 gtgttcgaac ttctctatag ctgactctac tgcaggggtg ttgagcattt gttgcgctac
    22141 atctaagggc agggtgatgg attcacatcc tgccagtaaa cagtccagcg cctgacacgg
    22201 cgttttaaag ctggctgcca gcaccatgct ttctggcgcg tgcatttcta acagcgtttg
    22261 cagctcctga accgtacgaa tgccgtcccc gccctgggca tctacgcggt taacatacgg
    22321 agcaacgtat tttgcccctg ccagtgcggc taataaccct tctgcggcgc tatatacagc
    22381 ggtgccaagt gtagtaatac cctctttttt cagtatttta attgctgcca gaccttcgga
    22441 agtcaccggg attttcacca caatacccgg aatagcgtcg cgcaggcgct tcgcttcttc
    22501 caccatcccc tgcgcgtcgc ggctcatggt ctgagcaaac agaatgccct catcaccaat
    22561 cgctttttgc agacgcggca gcacttccca gatggactcc ttgctggcag cgataatgct
    22621 cgggttagtt gtcaccccgg caatcgggaa tatgcgtgcc agacgttcga cttctgcgac
    22681 gttagcggtg tccagataca gttccatgat gtttcctttt acagttccag ttcatgttgc
    22741 agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg
    22801 tgcatgatgc ggcgagcgag gcgcgagaaa atgcgcatgt gctgatcgcc cgcagcgtgt
    22861 ttgttcaggg ttaacataat gatgaattgc gcttcatcat cgccccagcg caccggcgct
    22921 tgcagacgcg ccacgctgat ggtggattgc tcaatgtgtt ctgatttgct gtgtggaatg
    22981 gcaaaactaa agcccagacc ggtagagaaa acggcctcgc gcgcccataa gtcggcttcc
    23041 agtttacgcg gatagcggca gcggcccgcc agcagcaggt tatcggtcat gcctttgagc
    23101 acttcttctt tgctgcgcca gtcactttcc agcgtgatgc actcggcggt gaccagcggt
    23161 gcgtcctgtt gggtcatgcg gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg
    23221 caggccattg cctggttgag caacttgcgg cactcacggc tatcaagttg cgccatccgc
    23281 gctttcgccg ccggaattga tggtgcgctc atgctgagtt catccagccc taagccgacc
    23341 agcaacggca gcacggaacc tttcgctccc agctcaccgc acagaccaat ccatttaccc
    23401 tggcgatgca ccgcctgcac ggcgtaatcg agcgcccgca agaatgccgg attcaggctg
    23461 ttgtagtgac gagtaacctt agcgttatcg cgatccactg ccagcagata ttgcgtcagg
    23521 tcattactac caatactaaa gaaatcaatc tcttcgcagc attgatcgat gatgaacatc
    23581 accgacggca cttccagcat aatgccgagc tggatcttct catcaaacgg aatgtgttcg
    23641 ttacgtagtt gctgtttggc ttctgccagt ttttctttca cccataagat ctcttccatt
    23701 gaggagatca tcgggatcat gattttcagg ctgccgtgag cggaggcgcg caggatcgag
    23761 cgtagttgtg tggtaaataa cgacgcgtat tcttcataaa tacgcacggc gcgataaccg
    23821 aggaacgggt ttgcctctgc gggaatgttt agataatcaa cgggtttgtc gccgccaatg
    23881 tccatagtgc gcacaataat gctgcgtccg ttggcagact ccagcgcctg acaaaaaatg
    23941 ttgtacagct cgctttcgcc cggtgcactg gtgcgatcca tatagagcat ttcagtgcgg
    24001 aacaaaccaa cgccttccgc accattgccg aatgcggcct gcgcttccac ggagtgagcg
    24061 atgttagcgg caatttcaat gcggataccg tcagcggtac gggcttgttg ggtcagccag
    24121 acacgctgtt gctcacgcag ggcgtcctgt acgcgggctt cttgctgata ataacgagct
    24181 acggcttctc ctggctcaac cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga
    24241 tgctgccacg gagtgagggc atcaatatcc acaccaacca gcgtcggaat gttgaacgaa
    24301 cgggcaagga tcaccgtatg tgaggtggtg ccgccgcttt tcagtaacaa tcctttgagg
    24361 tgatttttat ccagttcgag gaactggctg ggggtcagtt catcagccat acaaatggcg
    24421 gactgcgtca gtttgcccgg tgccgggaag cgttgctcac cgtagatttg ctggagtaac
    24481 tggaagcaga cgtcgcgtac gtccagggca cgttcttgca ggtatctgct gctggaacgg
    24541 gcgaactctt cgcaaaagtg attcgcactg gcgacaatcg cttcggcgca gcttaatccg
    24601 gcgctgactc ctgccagtaa atgttcgtgc agggaagtat cgccagccag ggatcggtgc
    24661 gcttccagaa tcgcgctggt agcaccgtcg ctatccagca gacgaaactc aatgtttttc
    24721 agtaccagcg tcaggccgtt ttccagtgcg gattgctcgg cgtcaacgtc tttggctgcg
    24781 ggaagattac tcagcgcatt gagatctaaa gaagagatcg gcgtcagaat gccgcctgca
    24841 ctaccgctgc acacggtgcg gacgcggata atttgcggat tcagattggt cagtgaaacc
    24901 ggcagtggtt ccagttcgtc agatttaact tccgccagcg gcgcatcgca gtgggggaat
    24961 tcatcgcgca gccattggct taaacgctgg tgcccttcct gttcgtcggc cccggaaatc
    25021 agtagctggc agttatcacc caccagcgta tcggtgccaa tcagcgccag agcgcttttg
    25081 gcgttgcctt tacggtcagt acgcaggtta tgccactcaa tttgagatga aaaagtatta
    25141 cacagcgttt caacgtggct tgccggacgc gcatgtacgc cgttaggtag ctcacaaata
    25201 aattccacaa tcagggccat tgcctttctc ccggtacggt tttctgttta caggataaga
    25261 gcgcacggta acggcctgcc atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa
    25321 ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg gcatgcttcc ggattatcgc
    25381 aagttatgag gcggatcgca tttttgtact gatattacaa aagtccagta aatggccttt
    25441 ttatccactg tttgctccgc ctgcgattgc ctattgtttc ctccatcaaa aatatggaca
    25501 tgggcttttg caagcccggg agcagcttat gaatgagttg gtgcagatcc tgaaaaatac
    25561 ccgtcagcat ttaatgacgg gcgtttcaca catgattccc ttcgtggtat cgggcggtat
    25621 tttgctggcg gtttccgtca tgttgtatgg caaaggcgca gtgccggatg ccgtagccga
    25681 tccgaatttg aaaaaactgt ttgatattgg cgttgcaggt ttgacgctga tggtgccttt
    25741 cctcgccgct tacattggtt actccattgc agagcgttct gcgctggctc cgtgcgctat
    25801 cggtgcctgg gttggtaaca gctttggtgc gggcttcttt ggtgcgctga tcgccgggat
    25861 tatcggcggc atcgtggtgc attacctgaa gaaaattccg gtgcataaag ttctgcgctc
    25921 ggtgatgccc atcttcatta ttcctatcat cggcacactg attaccgcag gcatcatgat
    25981 gtggggcttg ggcgagcctg taggggcgtt gaccaacagc ctgactcagt ggcttcaggg
    26041 gatgcagcag ggcagcattg ttatgctggc ggtgatcatg gggctgatgc tggcgttcga
    26101 tatgggcggt ccggttaaca aagtggccta tgccttcatg ctgatttgcg ttgctcaggg
    26161 tgtttatacc gtggtggcca ttgctgccgt gggtatttgt atcccgccgc tggggatggg
    26221 gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa gagcgcgaaa ccggcaaagc
    26281 ggcactggtg atgggctgtg ttggcgttac tgaaggggcg attcctttcg ccgctgccga
    26341 tccgctgcgt gtcattcctt ccattatggt cggttcagtt tgtggtgcag taactgcggc
    26401 gctggtcggt gcgcagtgct atgcaggctg gggtggtctg attgtgctgc cggtggttga
    26461 aggtaagttg ggttatatcg cagcagtggc tgtcggagca gtggtgacgg ctgtttgtgt
    26521 gaacgtgctg aaaagtctgg cgcgtaaaaa tgggtcttcg actgatgaaa aagaagacga
    26581 cctggatttg gattttgaaa ttaattaatt gaggaacgag ccatgacgaa aattattgca
    26641 gtaaccgcat gtccttcagg tgttgcccat acctatatgg tagcagaggc gctggaaagt
    26701 gccgcgaaag cgaaaggctg ggaagtgaaa gtagaaaccc aggggtcaat tggtctggaa
    26761 aatgaactga ctgcggaaga cgtggcgagc gctgatatgg tgattctgac caaagatatc
    26821 ggcatcaagt ttgaagagcg ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat
    26881 gcagtaaaac gtgctgacgc cattatgagc aaaattgaag ctcacctggc gcaaaccgct
    26941 taagttcttc tcccgctcgc aagggcgggt tcgctttctc acaggagttc ctcatgacga
    27001 atcgtatctc tcgcctcaaa actgcactgt ttgccaatac ccgtgaaatc tcgctggagc
    27061 gggcgctgct ttataccgcc agccatcggc aaaccgaagg cgaaccggtg atattgcgcc
    27121 gggcgaaagc aacagcgtat atccttgaac atgttgaaat ttcgattcgt gatgaagaac
    27181 tgattgccgg taaccgcacc gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc
    27241 cttactggct gctgaaagag ctggatcaat tcccgacgcg tccgcaggac cgctttgcta
    27301 tcagcgaaga agataaacgt atctaccgtg aagagttgtt cccgtactgg gaaaaacgtt
    27361 cgatgaaaga tttcatcaac gggcagatga cagatgaagt aaaagccgcg accaacacgc
    27421 agattttcag catcaaccag acggataaag gccaggggca cattattatt gattacccac
    27481 gcctgttgaa tcacggtctg ggtgagctgg tggcacagat gcagcaacat tgtcagcaac
    27541 agccggagaa tcacttttat caggccgcgt tgttactgct ggaagcctcg cagaaacaca
    27601 ttttgcgtta cgccgaactg gcggaaacga tggcggcaaa ctgcacagat gcccagcgtc
    27661 gcgaagagct gctgactatt gcggagatct cccgccataa cgcgcaacat aagccgcaga
    27721 cgttctggca ggcgtgccag ttattctggt acatgaacat cattctgcaa tacgaatcca
    27781 acgccagttc gctgtcttta gggcgcttcg accagtatat gttgccgttc tatcaggcat
    27841 ctttaaccca gggcgaagat ccggcgttcc tgaaagaact gctcgaatct ttatgggtga
    27901 aatgcaacga catcgtgctg ttgcgctcca ccagtagcgc gcgttatttc gccggtttcc
    27961 cgaccggcta taccgcactg ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg
    28021 tgctttcgtt cctttgcctt gacgcctatc aaagcgtgca attaccgcaa ccgaacctcg
    28081 gcgtgcgcac taacgccttg atcgacacgc cgttcctgat gaaaaccgcc gaaaccattc
    28141 gcctcggcac cggtattccg caaatcttta acgatgaagt ggtggtgcca gcgttcctca
    28201 accgtggcgt ttcgctggaa gatgcgcgcg actattccgt agtgggctgt gtggaattat
    28261 ctattcccgg cagaacctac ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga
    28321 tggaaatctg cctgcatgaa aatgaaggca atgctgcgct gacttatgaa ggtttactgg
    28381 agcagatccg cgccaagatc agccactaca tcaccctgat ggttgagggc agcaatattt
    28441 gtgatatcgg ccatcgcgac tgggcacctg taccgctgct ctcgtctttt atcagcgatt
    28501 gtctggaaaa aggtcgcgat attaccgatg gcggcgcgcg ttataacttc tccggcgtac
    28561 agggaattgg tatcgccaac ctgagcgatt ctctccatgc gttgaaaggg atggtttttg
    28621 agcaacagcg tttaagtttt gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc
    28681 cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt tgagaaatac ggtaacgata
    28741 tcgacgaggt ggataacatt agcgccgaac tgttgcgcca ctactgcaaa gaagtggaaa
    28801 aataccagaa cccgcgcggc ggttacttca cgccgggatc gtataccgtt tctgctcacg
    28861 ttccgttggg atcggtggtt ggcgcgacgc cagacggtcg ttttgccggg gaacaactgg
    28921 cagacggcgg cttgtcacct atgctgggtc aggacgcaca agggccaacg gcggtactga
    28981 agtcagtcag taagctcgat aatacgctgc tgtctaacgg tacgttgctg aacgtgaaat
    29041 tcactccggc gaccctggaa ggtgaagcgg gattacgcaa actggccgac ttcttacggg
    29101 tgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc gacacgttgc
    29161 gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt gctggataca
    29221 gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc cggacagcgc
    29281 atcagctgta acgttgtgga aacgcgccgc gatgatgtgg cgcgcatttt caacattcag
    29341 cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa aggctgtccg
    29401 catctttgcc cgtggtgtgc taatccggag tcgatctccg gcaaaatcca gacggtacgc
    29461 agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc
    29521 ggggcgtttg aacggattgg tcgcgatatc agccttgacg ctctggaacg ggaagtgatg
    29581 aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg
    29641 ttaatgcagg cggagtttgc tacccgtttt ttacagcgac tgcggctgtg gggtgtctca
    29701 tgcgccattg aaactgccgg agacgcgcca gcaagcaagc tgttaccgct ggcgaaattg
    29761 tgcgatgaag tgctgttcga tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg
    29821 aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga gggtgtcaac
    29881 gtgatcccgc gtttaccgtt gatccctggt ttcacgctca gccgggagaa tatgcagcag
    29941 gcgctggatg tactgatccc gctgaaaatc aggcagatcc atctgttacc gtttcatcag
    30001 tacggcgaac cgaaataccg cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg
    30061 ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg atttcaggtt
    30121 accgtgggag gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa gcggcgtggc
    30181 gcatacttat atggcggcgg aacggctgga aaagttgtgc cagttagaga agtggggcgt
    30241 cagcattgaa actcagggcg cgctgggaac ggagaatcgt ttagcggacg aggatatccg
    30301 tcgggcggat gttgctctgt tgattacgga tatcgagctt gccggtgccg agcgatttga
    30361 acattgccgc tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc agcgggtaat
    30421 gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc tggagtagtc
    30481 ggtttttctg tcagttggct gtggtactgc cggcgatatt ccgacggcga gtgttcggtg
    30541 tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact
    30601 tcttttacct tcaggtcata gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga
    30661 ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga gagatagttt
    30721 ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg
    30781 tcgatataat cgcgaatagc ttcaaatagt gcctggctgc gtgaggcggt ctggatttgg
    30841 ctgccgcgca aatcgcggca gtggctaagc aggctggcta cgataagcct tgccgtttgc
    30901 tgctcctgcg gctgcatttg catttcattg agcgtttgta gcagaaaaga acctatgcgt
    30961 gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc atcccattgc
    31021 acgacgctga agccgagttg ctgtttgcca aacagcacgc taaaggtggt agcgggtgtt
    31081 ttccattgtg ggaaattcca gccaccagcg gcaacgtaca gcacatcgcc gggaactaac
    31141 gttgctccag cgccggtatc aacaaactca ccttccagca caatttccag ccgtggaaaa
    31201 tcgacctgat acgcaagatc gggcaccggg ccgttactgc tggcaaagta aatctggcgc
    31261 agggacagcg ggccgttgat caggcgggag agcaaatagc tgacgtcgtg atacatgtta
    31321 ttcccttagg tatgtagacg ggaaaacagt aactggcatt attaaaaaag gccacctgaa
    31381 aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca gtatcgcatc cggcaaatag
    31441 cgccagacgc tatacaaagg tcatctactg attacccacc tgatcgccat acggcagagt
    31501 gtcgtaatcg atcagcgcgt ttttcttgta cgggttacca atccagcggg tagtttcttt
    31561 gaactgcgga ttcaccactg aacgggttgg atcgtaaccg tcgtaagata agcccgccaa
    31621 atctgaccag gtgtggatca gttccgccag gctgtattta cgatcgacat cctgtgagaa
    31681 atcacgggga tgagtcgctt gccatttttc cgacgtccac agcaggaacg gaatggtgta
    31741 catgtggcgc gtcgggttgt cttcgttacg cccctgagtt ttgtgcggcg gcgtgtcgta
    31801 aacctcttca ccgtggtcag agaagtaaac caggaatccg ttcgggtctg ccgctttaaa
    31861 gtctttaatc aggctggcaa ccacatgatc gttatacagg ttagcgttgt cataatcgtt
    31921 atatgattcc agctcttctg cgcttaatcc tggaggaaca tgatcggtat tgccatcaaa
    31981 cttgccctga ttttccgggt agcggtattt gtatttgata tgcgtaccca gcagatgaac
    32041 gatgatcagt ttcttcggcg cagggtcatt cagcacatcc tggaacggct tcagcacgtt
    32101 ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc atgtagtact gcttgtcggt
    32161 ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc atcgtctgct ggttggtgat
    32221 ccagaaggtt ttataacccg cctgtttcat catgttcatc agcgacggct gcgtcagata
    32281 cagatccggg ttcttttcat tggcaaaggt cagcgcctgt tgcaggattt caatggtgta
    32341 cggacgagac gtaactacgt tattaaacac ggtcagattc ggatcggttt tatgcagcgc
    32401 atccagctcc ggcgtggttt cacgcggata accgtacaga ctcatgcgtc cgcgctgggt
    32461 cgactcgcca atcaccagca ctaaagtgcg cggttcgtta cccgattcat ctttgaaatt
    32521 agccagtggc ggcaaggcat tattttcatt cagtaacttt gttagcgagt ttagttgctg
    32581 acgatactga taatagccgg tcaggaattg ccacggtgcg gcaggctcca tacgcgaggc
    32641 taggttatcc aacgttttct caaacggctt gtttttgata aacgtattca tggcgatcgg
    32701 atgcagaatc aagccataaa gcagggcaaa agagacaaca taacgccacg gctttggaat
    32761 atagaccggg cgcaggcgtg tccacagcag aactgccacc gccgtatagg ccagcgcgat
    32821 aagcacaatt ttcaggctga aatactggct taaatactcg ctggcttcgt tggtgttggt
    32881 ttcgaacatc acaaacagaa cgctctgcga gaactcctgc ccgtagatga cgtagtagca
    32941 cagcgccgcc agagaggccg tccatagcac cacgccgatc actgcggcaa taattttaat
    33001 ccgcttcgga aagaggaata ccgggatcaa ccacagcgag ctgaataaca gcgagtcgcg
    33061 aatgccgttg gtgccactat aaccactgat gtaaataatg gcctgtagca gagtagagaa
    33121 aaaccaaaag tagagcagtg cccaacccag ggctttccag ctagaaaggg gtttagcctg
    33181 gacttctgtg gaatgcatag taagaacctg tcttgaaaaa aatatcgccg aatgtaacga
    33241 caattcctta aggatatctg aaggtatatt cagaatttga ataaaatgca gacagaaata
    33301 tattgaaaac gagggtgtta gaacagaagt atttcagaaa accctcgcgc aaaagcacga
    33361 gggtttgcag aagaggaaga ttagccggta ttacgcatac ctgccgcaat cccggcaata
    33421 gtgaccatta acgcttgttc gacgcgagga tccggttcct ggccttcttt ttctgcctgg
    33481 cgggagcggt gcagcaactc ggcctgcaat acgttcagcg ggtcggtgta aatattccgt
    33541 agctgaatag actctgcaat ccacggcaga tcggccatca gatgggaatc gttggcaatc
    33601 gccagcacca ctttgatgtc ttcttcttgc aggttgcgta actctttacc taacggccac
    33661 agtgctttgt ctaccagacg ttgatcatag tattccgcca accacaggtc tgctttggcg
    33721 aagaccatct ccagcatgcc gagacgcgtc gagaagaatg gccaatcgcg gcacatggct
    33781 tccagctcgc tctgtttgcc atcttcgacc actttttgca gcgccgtacc tgcacccagc
    33841 caggcgggga gcatcaggcg gttttgcgtc caggcaaaaa tccacggtat ggcgcgcagc
    33901 gactcgacgc cgccggttgg gcgacgtttc gccggacgtg aacccaacgg cagtttgccc
    33961 aattcttgtt ccggcgtagc ggagcggaag taaggcacaa aatctttgtt ttcacgtacg
    34021 tagccacggt agagatcgca ggagatgact gacagttcat ccataatgcg acgccagctc
    34081 tctttcggct caggtggtgg cagcaggttg gcttccagaa tcgccccggt gtaaagcgac
    34141 aggctgctga cggtgatttc tggcagacca tatttaaagc ggatcatctc gccctgttca
    34201 gtcacgcgca ggccaccttt caggcttcct ggcggttgtg acagcagcgc cgcatgggca
    34261 ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga acaacgtcag ctcaataccc
    34321 gctttttcgc aggttttgat taatgcatcc tgtgcctgat attgcgccca ggaagctgcc
    34381 atcacgcccg catcttttgc tgagtcggaa tagccaatca tcaccatctg tttgccctga
    34441 atcaggccgc gataccagtc gatattgagc agctgggtca tgacatcgtt ggcgttgttc
    34501 aggtcatcga gggtttcaaa cagcggagca accggcatcg caaacccgat acccgcttct
    34561 ttcagcagca ggtggacagc cagtacgtcg gacggcgttt tcgccatcga gatcacgtag
    34621 gcggcaatgg agccttgcgg cgcttcggca atcacctggc aggtatcgag cacttcgcgc
    34681 gtttcggcgc ttggttgcca gttgcgcggc agaagcggac gtttggagtt cagttcgcgg
    34741 atcaggaacg cctgtttgtc ggcctctgac cagctttcgt agtcgccgat accgaggtag
    34801 cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct cctgacggat atcaatacgg
    34861 accagcggta cgccgaaaca tttcacgcgg cgcagggtgt cgagcagatc gccgttggcg
    34921 ataataccca tgccacacgc ctgaagtgac tggtagcaag cgtagagcgg ttcccacagt
    34981 tcttcgtttt gtgtcagcag cccttctggt tttggcagtt cttcgccttt caggcgcgct
    35041 tccagccatg cctgtgtcgc catcaggcga gaacgcaggt ttttcatcag atagcgatac
    35101 ggttctgcgg caccttcttc gccaaccagc gccagcagtt caggggtcgc ttcaaccatc
    35161 gacagttcag aaaccagaac ctgaatatct ttcaggaaca gatcggtggc tttccagcgg
    35221 ctgagcagca ggacgtggcg ggtgatatcg gcagtgacgt tcgggttgcc gtcgcggtca
    35281 ccgcccatcc acgaggtaaa acgtaccgga acaaattcga cgggcagttt gtagtcgaga
    35341 ttctcttcca gttgttcgtt cagttcgcgc aggtaattcg gtacgccttg ccacaggctg
    35401 ttttctacta cggcaaagcc ccatttggct tcatctaccg ggcttggacg cagcttacgg
    35461 atttcatcgg tatgccatga ctgggcgatc aactggtgca ggcgacgcat cagctggttg
    35521 tgttcgtagt cagcgatatc tttgttatcg agctgtttta aacaggcgtt cacttccacc
    35581 attttgtgga tcagtgtacg acgggtaatt tcggttgggt gagccgtgag gaccagttcc
    35641 agcgacagcg attccactgc ttttttgatg gtgtcttcgc tcagttccgg ctggtttttc
    35701 agtttacgca gggtgcgggc gatcacttcc gggttgctgg cagcttcgcc tttcggcgaa
    35761 atgctgtggt attgctcggc ggtgttggcc aggttcagga actgactaaa cgcacgcgca
    35821 acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg tgagcaactc ctggcggtta
    35881 gcatcattgc cagcgcgtga agatttcgac aacttacgga tagtttctac gcgttcaaga
    35941 atgtgttctc ccaacgcatc cttgatggtt tctcccagca ctttgccgag catactgaca
    36001 ttactacgca atgcggaata ttgttcgttc atattacccc agacacccca tcttatcgtt
    36061 tgatagccct gtatccttca cgtcgcattg gcgcgaatat gctcgggctt tgcttttcgt
    36121 cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg ctgcgaaatc gcttcagcaa
    36181 acgaataaat agcaggaatt tacgccatta aattcacgac gctttaaata agcgtaactt
    36241 atggaaatgt taaaaaatcg ccatacataa caccaaaggt gtaggtcgga taagatgcgc
    36301 aagcatcgca tccgacatta ttgcggcacc ggagtttggc aacggtgccg gatgcggcgc
    36361 gagcgcctta tccggcctac gttttaatgc cagcaaaaat ggtgaattac ctggataatc
    36421 aactcacggg tgggtttaat aaaccgcgtc tccagatatt catcaggctg atgagcttga
    36481 ttgatggaac ccggaccgag caccagcgtc gggcataacg tttgaataaa cggcgcttcg
    36541 gtacagtaat tcaccacttc ggtttttgct ccgagcaatt tctcaaccac ttcaaccagt
    36601 tgatgattcg gtgggcattc atagccaggg atcggcggat gcagctcgtc gaccgtcaga
    36661 cgacccggcc agcgttcgct caccggagcc aatgcatcgt tgagcaaacc attaagttca
    36721 ttgagtgtca tgccaggctg cggacgaata tccatatgca actcacagca agcgcaaata
    36781 cggttagaag cgtcgccacc gtgaatatgc ccgaggttga gcgtagggta tggcacggta
    36841 aatgcttcgt agtgataacg ttctttcagg ttatcgcgca attgcaaaat atgcccgatg
    36901 gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg gatcgctgga gtgccccgac
    36961 tggccctgaa tacggatggc gttagagata tgacctttat gtgcgcgtac cggttgtagt
    37021 gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg cggtagtttc ggcaaaataa
    37081 cgcgctccgg ccatactggt ttcttcatca gcagtcgcca gaatgtagag cggttttttc
    37141 agtttcgtga cgtcgacatc gcgtagcgca tcaaggataa acgcaaaaaa gcctttcatg
    37201 tcggcggtgc ctaagccgta aagcttgccg tcatgctccg tcagtgtaaa cggatcgcgc
    37261 gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc ccgccagcaa caagccgcca
    37321 gccccctgtc cgtaactggc cagcatatta aatttgttgc gagttcctgg aacaggctgc
    37381 acttccacat tgaagcccaa atctttaaac cagtccgcca gcagagtgat taaatctgca
    37441 ttgctttgat cgagtgcctc ttccgtagcg cttattgaag gtgtggcaat cagagcgcgg
    37501 taaatctcga taaatggcgg taatttgttt ttcattgttg acacacctct ggtcatgata
    37561 gtatcaatat tcatgcagta tttatgaata aaaatacact aacgttgagc gtaataaaac
    37621 ccaccagccg taaggtgaat gttttacgtt taacctggca accagacata agaaggtgaa
    37681 tagccccgat gttgaatacg ctgattgtgg gtgccagcgg ctacgctggc gcagagctag
    37741 tgacctatgt aaatcgccat ccgcatatga acataaccgc tttgactgtt tcagcgcaaa
    37801 gcaatgatgc gggaaagtta atctccgatt tgcatccgca gctaaaaggc atcgttgagc
    37861 tgccgttgca gccgatgtcg gatatcagcg agtttagccc aggggtggac gtagtgtttc
    37921 tcgccaccgc ccatgaagtt agccacgatt tagcgccgca gtttcttgaa gcgggctgcg
    37981 tggtgttcga cctttccggc gcgtttcgtg ttaacgacgc caccttctat gaaaaatatt
    38041 acggctttac ccatcaatac ccggaactgc tggaacaggc agcctacggt ctggcggagt
    38101 ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt gccaggctgt tatccgacgg
    38161 cggcacagct ggcgctgaaa ccgttgattg atgccgatct tcttgacctc aatcagtggc
    38221 cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg taaggcagcg atttcaaata
    38281 gtttttgtga agttagcctg caaccgtatg gcgtctttac tcatcgccat caaccagaga
    38341 tcgccacaca cctcggtgct gacgttatct tcaccccaca tctgggcaat ttcccgcgcg
    38401 gcattctcga aaccattacc tgccgcctga aatcgggtgt gacccaggcg caagtcgcgc
    38461 aagcgttaca gcaggcgtat gcccataaac cgctggtgcg gctgtatgac aaaggcgttc
    38521 cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat cgggtttgcc gttcagggcg
    38581 agcatctgat tattgtggcg accgaagaca acttactgaa aggcgcggcg gcacaagcgg
    38641 tacagtgcgc caatattcgt ttcggctatg cggaaacgca gtctcttatt taagggtgca
    38701 atgatgaatc cattaattat caaactgggc ggcgtactgc tggatagtga agaggcgctg
    38761 gaacgtctgt ttagcgcatt ggtgaattat cgtgagtcac atcagcgtcc gctggtgatt
    38821 gtgcacggcg gcggttgcgt ggtggatgag ctgatgaaag ggctgaatct gccggtgaaa
    38881 aagaaaaacg gcctgcgggt gacgcctgct gatcagatag acattatcac cggagcactg
    38941 gcgggaacgg caaataaaac cctgttggca tgggcgaaga aacatcagat tgcggccgta
    39001 ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc agcttgatga agagttaggt
    39061 catgttggac tggcgcagcc aggttcgcct aagcttatca actccttgct ggagaacggt
    39121 tatctgccgg tggtcagctc cattggcgta acagacgaag ggcaactgat gaacgtcaat
    39181 gccgaccagg cggcaacggc gctggcggca acgctgggcg cggatctgat tttgctctcc
    39241 gacgtcagcg gcattctcga cggcaaaggg caacgcattg ccgaaatgac cgccgcgaaa
    39301 gcagaacaac tgattgagca gggcattatt actgacggca tgatagtgaa agtgaacgcg
    39361 gcgctggatg cggcccgcac gctgggccgt ccggtagata tcgcttcttg gcgttatgcg
    39421 gagcagcttc cggcactgtt taacggtatg ccgatgggta cgcggatttt agcttaagtt
    39481 ttgttagccg gaggagcagc tttccggcat tgaatttcaa aataaggaaa cagagttatg
    39541 gcactttggg gcgggcgttt tacccaggca gcagatcaac ggttcaaaca attcaacgac
    39601 tcactgcgct ttgattaccg tctggcggag caggatattg ttggctctgt ggcctggtcc
    39661 aaagccttgg taacggtcgg cgtgttaacc gcagaagagc aggcgcaact ggaagaggcg
    39721 ctgaacgtgt tgctggaaga tgttcgcgcc aggccacaac aaatccttga aagcgacgcc
    39781 gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca aagtgggcca gttaggcaaa
    39841 aagctgcata ccgggcgtag ccgtaatgat caggtagcga ctgacctgaa actgtggtgc
    39901 aaagataccg ttagcgagtt actgacggct aaccggcagc tgcaatcggc gctggtggaa
    39961 accgcacaaa acaatcagga cgcggtaatg ccaggttaca ctcacctgca acgcgcccag
    40021 ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga tgctggcgcg tgatgaaagc
    40081 cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat taggctgtgg cgcgctggcg
    40141 ggaacggcct atgaaatcga ccgtgaacag ttagcaggct ggctgggctt tgcttcggcg
    40201 acccgtaaca gtctggacag tgtttctgac cgtgaccatg tgttggaact gctttctgct
    40261 gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag atctgatttt ctttaacacc
    40321 ggcgaagcgg ggtttgtgga gctttcggac cgcgtgactt ccggttcatc attaatgccg
    40381 cagaagaaaa acccggatgc gctggagctg attcgcggta aatgcggccg ggtgcagggt
    40441 gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc tggcttacaa caaagatatg
    40501 caggaagaca aagaaggtct gttcgacgcg ctcgatacct ggctggactg cctgcatatg
    40561 gcggcgctgg tactggacgg cattcaggtg aaacgtccgc gttgtcagga agcggcgcag
    40621 caggggtatg cgaacgccac tgaactggcg gattacctgg tggcgaaagg cgtacctttc
    40681 cgcgaggcgc accatattgt gggtgaagcg gtggtggaag ccattcgtca gggcaaaccg
    40741 ctggaagatc tgccactcag tgagttgcag aaattcagtc aggtgattgg cgaagatgtc
    40801 tatccgattc tgtcgctaca atcgtgcctc gaaaagcgtg cggcaaaagg cggcgtctca
    40861 ccgcagcagg tggcgcaggc gattgctttt gcgcaggctc ggttagagta atttgtttat
    40921 attgctcccc tgaaaatggg gagtttacca cgcctacaaa gtgcgacact tcgtaggcgg
    40981 ataaggcgtt tacgccgcat caggcagaaa ttt
//
LOCUS       DAJTRQ010000019        38755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000019 DAJTRQ010000000
VERSION     DAJTRQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO332_001155"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OO332_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5941712.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..579
                     /gene="hisL"
                     /locus_tag="OO332_001156"
     CDS             529..579
                     /gene="hisL"
                     /locus_tag="OO332_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5941713.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    555..678
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            725..1624
                     /gene="hisG"
                     /locus_tag="OO332_001157"
     CDS             725..1624
                     /gene="hisG"
                     /locus_tag="OO332_001157"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5941714.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1630..2934
                     /gene="hisD"
                     /locus_tag="OO332_001158"
     CDS             1630..2934
                     /gene="hisD"
                     /locus_tag="OO332_001158"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5941715.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2931..4001
                     /gene="hisC"
                     /locus_tag="OO332_001159"
     CDS             2931..4001
                     /gene="hisC"
                     /locus_tag="OO332_001159"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5941716.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4001..5068
                     /gene="hisB"
                     /locus_tag="OO332_001160"
     CDS             4001..5068
                     /gene="hisB"
                     /locus_tag="OO332_001160"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5941717.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5068..5658
                     /gene="hisH"
                     /locus_tag="OO332_001161"
     CDS             5068..5658
                     /gene="hisH"
                     /locus_tag="OO332_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5941718.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5658..6395
                     /gene="hisA"
                     /locus_tag="OO332_001162"
     CDS             5658..6395
                     /gene="hisA"
                     /locus_tag="OO332_001162"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5941719.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6377..7153
                     /gene="hisF"
                     /locus_tag="OO332_001163"
     CDS             6377..7153
                     /gene="hisF"
                     /locus_tag="OO332_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5941720.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7147..7758
                     /locus_tag="OO332_001164"
     CDS             7147..7758
                     /locus_tag="OO332_001164"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5941721.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="OO332_001165"
     CDS             complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="OO332_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5941722.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="OO332_001166"
     CDS             complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="OO332_001166"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5941723.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="OO332_001167"
     CDS             complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="OO332_001167"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5941724.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11998..12357)
                     /locus_tag="OO332_001168"
     CDS             complement(11998..12357)
                     /locus_tag="OO332_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941725.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(13007..13570)
                     /locus_tag="OO332_001169"
     CDS             complement(13007..13570)
                     /locus_tag="OO332_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941726.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="OO332_001170"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="OO332_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="OO332_001171"
     CDS             complement(14779..15153)
                     /locus_tag="OO332_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5941727.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="OO332_001172"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="OO332_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5941728.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17230)
                     /gene="rfbG"
                     /locus_tag="OO332_001173"
     CDS             complement(16325..17230)
                     /gene="rfbG"
                     /locus_tag="OO332_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5941729.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="OO332_001174"
     CDS             complement(17223..18113)
                     /locus_tag="OO332_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5941730.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="OO332_001175"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="OO332_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5941731.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="OO332_001176"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="OO332_001176"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5941732.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="OO332_001177"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="OO332_001177"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5941733.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="OO332_001178"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="OO332_001178"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5941734.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="OO332_001179"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="OO332_001179"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5941735.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="OO332_001180"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="OO332_001180"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5941736.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="OO332_001181"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="OO332_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5941737.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="OO332_001182"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="OO332_001182"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5941738.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="OO332_001183"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="OO332_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5941739.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="OO332_001184"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="OO332_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5941740.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="OO332_001185"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="OO332_001185"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5941741.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="OO332_001186"
                     /gene_synonym="manB"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="OO332_001186"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5941742.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="OO332_001187"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="OO332_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5941743.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="OO332_001188"
     CDS             complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="OO332_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5941744.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35472..35951)
                     /gene="gmm"
                     /locus_tag="OO332_001189"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35472..35951)
                     /gene="gmm"
                     /locus_tag="OO332_001189"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5941745.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="OO332_001190"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="OO332_001190"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5941746.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="OO332_001191"
     CDS             complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="OO332_001191"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5941747.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="OO332_001192"
     CDS             complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="OO332_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5941748.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38633..>38755)
                     /locus_tag="OO332_001193"
     CDS             complement(38633..>38755)
                     /locus_tag="OO332_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5941749.1"
                     /translation="CADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10350 a         9688 c         8721 g         9996 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
      601 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
      661 ttccgggggc ttttttattg cgcggttgtt aacggttcag acaggtttaa agaggaataa
      721 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
      781 tgactcacgc gaattactgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
      841 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg atgacattcc
      901 cggtctggta atggacggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga
      961 agagctgctt aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgtct
     1021 ggatttcggc ggctgccgcc tttcgctggc aacgccggtt gatgaagcct gggacggtcc
     1081 gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
     1141 cgaccagaaa ggtatctctt ttaaatcctg cttactgaac ggttctgtcg aagttgcgcc
     1201 gcgtgccggg ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
     1261 taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga
     1321 tggcgaaatg gaagaatcta aacagcaact gatcgacaag ctactgaccc gtattcaggg
     1381 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgcctaga
     1441 tgaagtcatc gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga
     1501 tcaacagcgc gtagcgatgc acatggtgag tagcgaaacc ctgttctggg aaacgatgga
     1561 aaaactgaaa gcactgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga
     1621 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
     1681 cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg caccgttaac
     1741 gatattctcg ataacgtgaa aacgcgtggc gatgaggccc tgcgtgaata cagcgcgaag
     1801 tttgataaaa ccacggttac cgcgctgaag gtgtctgctg atgagatcgc cgccgccagc
     1861 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagag tattgaaacc
     1921 ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc
     1981 cagcaggtca cgcgcccggt ggcttcagtg ggtttgtata ttccgggcgg ctccgccccg
     2041 ctcttctcaa cggtattaat gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg
     2101 gtgctgtgct caccgccgcc gattgccgat gaaattcttt atgcggcgca gctgtgcgat
     2161 gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg
     2221 gaatctgtgc cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagca
     2281 aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
     2341 gaagtgctgg tgattgctga cagcggcgct acgccggatt ttgtggcttc tgatttgctt
     2401 tctcaggctg aacacggccc tgactcacag gtaattttac tgacccccga tgctgatatg
     2461 gcgcgtcgcg ttgccgaggc cgtcgaacgc caactggcag aactgccgcg tgccgaaacc
     2521 acccgccagg cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgtg
     2581 gagatctcca accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa
     2641 ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg
     2701 gcaggcgact acgcctccgg caccaaccac gtgctgccga cttacggtta caccgccacc
     2761 tgttccagcc tcgggctggc agatttccag aaacgcatga ccgtacagga actgtcgaaa
     2821 gaaggtttct ccgcgctggc ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc
     2881 gcccacaaaa atgccgttac tttgcgtgtt aacgtcctta aggagcaagc atgagcaccg
     2941 tgactattac tgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc
     3001 gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatatccc acagccgtgg
     3061 agtttaagct tactcagcaa acgctcaacc gctacccgga atgccagcca aaagcggtga
     3121 ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggtg
     3181 cggacgaagg tattgaactg ctgatccgcg ctttttgcga accgggtaaa gacgccatcc
     3241 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt ggtgtcgaat
     3301 gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt tccgacaagc
     3361 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa ccccaccggg caactgatca
     3421 atccgcagga ttttcgcacc ctgctggagc taacccgcgg taaggcgatt gtggttgccg
     3481 atgaagctta tatcgagttt tgcccgcagg catcgttagc tggctggctg gcggaatatc
     3541 cgcacctggc gattttgcgt accctttcga aagccttcgc gttggcgggg cttcgttgcg
     3601 gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc cccccctacc
     3661 cgctctcgac gccggttgcc gacattgccg cacaggcgtt aagcccgcag ggaatcgtcg
     3721 ccatgcgcga acgggtggcg caaattatta cagaacgcga atacctgatt gccgcactga
     3781 aagagatccc ctgcgtggag caggttttcg actctgaaac caactacatt ctggcgcgct
     3841 ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
     3901 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
     3961 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
     4021 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
     4081 tgacaaactc gcctttgaac cgggcgtgat cccggaattg ctgaagctgc aaaaagaggg
     4141 ctacaagctg gtgatgatca ctaatcagga tggtctggga acacaaagtt tcccgcaagc
     4201 ggattttgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt
     4261 tgatgaagtg ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa
     4321 agtaaaactg gtggagggtt atctggctga gcaagcgatg gatcgtgcca acagttatgt
     4381 aattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg
     4441 ctacgaccgc gaaaccctga actggccaat gattggcgag caactcacta aacgagaccg
     4501 ttacgcccat gtagtgcgca acaccaaaga gacgcagatt gacgtccagg tgtggctgga
     4561 tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc tttgatcaca tgctggatca
     4621 gatcgccacc cacggcggtt tccgcatgga aatcaatgtc aaaggcgacc tctatatcga
     4681 cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaattgccct
     4741 cggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg aatgccttgc
     4801 ccgctgcgca ctggatatct ctggtcgccc gcacctggaa tataaagccg agtttaccta
     4861 ccagcgtgtg ggcgatctca gcactgaaat gatcgaacac ttcttccgtt cgctctctta
     4921 caccatgggc gtgacactac acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
     4981 aagcctgttc aaagcctttg gtcgtaccct gcgccaggcc atccgcgtcg aaggcgacac
     5041 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc
     5101 aacctgaact ctgtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt
     5161 gacccggaca ttgtgttgct ggccgataaa ctgtttttac ccggcgtcgg caccgcacaa
     5221 gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
     5281 ccggtgctgg gtatctgctt agggatgcaa ctcctgggac gacgcagcga agagagcaat
     5341 ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtctg
     5401 ccactgccac atatgggctg gaaccgcgtt tatccgcagg caggcaaccg cctgtttcag
     5461 gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg
     5521 tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac
     5581 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa
     5641 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc
     5701 gtctccatca gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccgcgct
     5761 tacaggatta cgctgcgcaa ggtgccgaag tgttgcacct ggtggatctg accggggcaa
     5821 aagatccggc taagcgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgtcc
     5881 cggtgcaggt tggcggcggt gttcgtagtg aagaagacgt ggcggcgtta ctggaagctg
     5941 gcgttgcgcg cgtggtggtc ggttccaccg cagtgaaatc accggacgta gtgaaaggct
     6001 ggtttgaacg tttcggtgcc gatgcgctgg tgctggcact ggatgtccgt attgacgagc
     6061 aaggcaacaa gcaagtggca gtcagcggct ggcaagagaa ctcgggcatt tcactggaac
     6121 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gttgtgtacc gatatctcgc
     6181 gcgacggcac gctggcggga tctaacgtct ctttatatga agaagtgtgt gccagatatc
     6241 cgcaagtggc gtttcagtcc tccggcggca ttggcgacat taacgatgtt gctgccctac
     6301 gtggcactgg cgtacgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg
     6361 tgaaggaggc catcgcatgc tggcaaaacg cataatcccg tgtctcgacg ttcgtgatgg
     6421 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccact
     6481 ggcaaaacgc tacgccgaag aaggtgcaga tgaactggtg ttctacgata tcaccgcttc
     6541 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
     6601 tccgttttgt gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt
     6661 tggcgcggat aaaatttcca tcaactcccc tgcgctggca gacccaacat taattactcg
     6721 cctggccgat cgctttggcg tgcagtgtat tgtggttggt attgatacct ggtacgacgc
     6781 cgaaaccggt aaatatcatg taaatcaata taccggcgat gaaagccgca cccgcgtcac
     6841 tcaatgggaa acgctcgact gggtagagga ggtgcaaaaa cgcggcgccg gagaaatcgt
     6901 cctcaacatg atgaatcagg acggcgtgtg taacggttac gacctcaagc agctgaaaaa
     6961 agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt ggcgcgggca ccatggaaca
     7021 cttcctggaa gccttccgcg atgccgacgt tgacggtgcg ctggcagctt ccgtattcca
     7081 caaacaaata atcaatattg gcgaattaaa agcgtacctg gcaacacagg gcgtggagat
     7141 caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggtctg
     7201 atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg ctacatgaac
     7261 ccggaagcct tagacaaaac catcgaaagc ggtaaagtca ccttcttctc gcgcactaaa
     7321 cagcgactgt ggaccaaagg cgaaacctcg ggcaattttc tcaatgtggt aagtattgcc
     7381 ccggactgcg acaacgacac attactggtg ctggcgaatc ccatcgggcc aacctgccac
     7441 aaaggcacca gtagctgctt cggcaacacc gctcaccagt ggctgttcct gtatcaactg
     7501 gaacaactgc tcgccgaacg taaatatgcc gacccggaaa cctcctacac cgccaaactg
     7561 tatgccagcg gcactaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca
     7621 ctggcagcaa cggtacatga tcgctttgag ctgaccaacg aggcatctga cttgatgtat
     7681 cacctgctgg tgctgttgca ggatcaggat ctggatttaa cgacagtaat tgagaacctg
     7741 cataaacggc atcagtgagt tacgggctga gcaggtgtga tgttgttgtc acacccggag
     7801 ccattgcccg atgcgcaaac ttatcgtgcc tacacattat ctcaacacac gtaggccgga
     7861 taagatgcgc cagcatcgca tccggcaaaa aaaacgggca tggtgtcacc accctgcccg
     7921 tttctcttaa atgcacaata atattacttc gcgttgtaat tacgcagagc gttacgcccc
     7981 aacacaatcc ccgcgccaac catgccgccc agaagcactg ccagaatcaa ggtgattgcc
     8041 tttttcggac tatcgcgacg aataggtaac gtcggtttca tcacatagcg gtaagcatga
     8101 atatcaagat catcaaactt taatttttca atatccaaca ggttttgacg tgtctgatag
     8161 tagtttggtg agaacaccaa cggacgagtc gcttcatgct taatcatcga ctccagcgct
     8221 tcgctcccta gaaggaacaa cgtatcttgc gtcacatctt cagtctgctg aacctgtggc
     8281 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
     8341 tctttctgct cctgcgcgac cacttcctgg gttcttaaag agtcctgcaa gtttttccgt
     8401 cccagagcaa tgttgtcctt gagatccttt tctagctctt gattcacttt atcatcaact
     8461 tgctgaatgt attgggccaa cttcatttgt gcgccctctg cagtttgccc aacataagaa
     8521 acagtcaatg gtaattgttg gttcttaaca gaaggttcga tggtaagttt ttctggctct
     8581 tcctgattat ccagcgtttc tgctaatgca gagaaggcag aactgaagcg accaattaac
     8641 gtctcctgca aatccgatac tttcggtgca gcctgaccat agataacatt catggcattg
     8701 ttatagccag caatttgccc cacgtcgggc tgagtgataa ttgctgttga cgtccatttc
     8761 tccttcgcta ctgccaaata accaatagcc agggcaatag ccacaatgac ggaaatgata
     8821 attgtcatct tgccacgcca caactgcact agtaaatcaa tcaaatcaat ctgttccggg
     8881 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aactgacgaa
     8941 aatttccaag tccgcatagt ttagcgataa tccccgtgaa tgctatagga taagtgataa
     9001 aaataatgaa ttattgcgga ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac
     9061 aagatgttag tcgctgccaa agagatcgcg ggtgtagacc ttatctgcca catccttaag
     9121 ctcttctgcc atacggttgg agataatgac gtcggcttgt tgtttgaagg tggcgagatc
     9181 acgttccagg cgagagttga agaatgagtc ttctttcatc accggctcgt agatgatcac
     9241 ttcaacgcct ttcgccttga tacgcttcat aatcccctga atagaagacg tacggaagtt
     9301 atctgacccg ctcttcataa tcagacgata aatacccacc acttgcggtt tgcgtgacaa
     9361 aatggcatcg gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgagatcag
     9421 gttattcggc acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata
     9481 accaccataa ccaaacgacg gattgttgta atggttgcca atacgcggat cgagacaaac
     9541 gccttcgata atctggcgag tattcagacc taaactttct gcataactat ccagttcatt
     9601 aaagtatgct acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc
     9661 agtggagtcg gtaaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
     9721 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgacg gatggagatt
     9781 atcgtaaagg gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg
     9841 atatttctta tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac
     9901 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
     9961 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
    10021 gtagtcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
    10081 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
    10141 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
    10201 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
    10261 tgaatcatga tgtttttagc atcgtgataa gatgctaaaa gttttaacgc tacgttgtca
    10321 gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct gatgtttatt
    10381 aagttaatat taagcagtga aatttagtct ataagatatt tggcaaaaaa aagcccggtg
    10441 caatatacgc cgggcctcaa ttttattgtt ggttaaatca gattaatcca gccattcagt
    10501 atggaacaca ccttctttat caatgcgctt ataagtatgc gccccgaaat agtcacgctg
    10561 tgcctggatc aggttcgcag gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc
    10621 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcgacga catcgcgcag
    10681 cgcctgctgg tagtcatcgg caatttgctt gaagtacggt gccagcagca ggttagcgat
    10741 ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac ggatgatgca
    10801 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt attcttcaga
    10861 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    10921 actgcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    10981 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    11041 agactcggta atcagcgaca gcggttcgcc gagatccagc gcgctctggc tggtccattt
    11101 accggtgcct ttgtttgctg cttcatccag aatcacatca accaggtagt taccgtcttc
    11161 atcttttttg gtgaagatat ctttagtaat gtcgatcagg tagctgctaa gttcaccgtt
    11221 attccactcg gtgaaagtct gggccagttc ttcattagat agattcaggc cacctttgag
    11281 cagagaatac gcttcagcaa tgagctgcat atctccgtat tcaataccat tgtgaaccat
    11341 cttcacataa tggcctgcgc cgtcggcacc aatataagta acgcatggct cgccgtcttc
    11401 tgccacggca gcgattttag tcaggatagg cgcaaccagc tcataagcct ctttctgccc
    11461 gccaggcata atagaaggac ctttcagcgc gccctcttca ccgccggaaa caccggtacc
    11521 gatgaagtta aagccctctg ctgaaagctc acgattacga cgaatggtgt cctggaagaa
    11581 ggtattacca ccatcaatga tgatgtcacc tttatcgagg tatggtttca gggaatcaat
    11641 cgcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    11701 agattcaaca aactctttca ccgtatagta aggagccagt ttcttgcctg gattttcagc
    11761 aatcacttct tccgtctttt cgcgggagcg gttgaaaata gagacggtat aaccacgact
    11821 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    11881 ctttgacatt gtttactcct gtcttctata ttattaaccg tctattaacc gttatattat
    11941 taaccgtcgt gattttcatc ttctaatcaa ggaatacata tggttacagc atgcttctta
    12001 ctttatagcg ataagtatgc cattgggtgt atccataaga actttgaatt ttggatactt
    12061 ttctggatta aaattagcat agcttcgttt agatataaca ataaacgaac tattaaaaga
    12121 tttcatcttc tctattaaat catcctgtgg gattacctca cactcgggat tactaatagt
    12181 ccaatcacta tatcttgcta atccaaagac gtcaccaatt gccatccctt tatagaagta
    12241 aataccatcc tccaagccta attggataag attatctcca tataactcac tatattcact
    12301 tgctttcttc atcagttcat agctatagcg actttctaag cgtgaattaa aattcaagta
    12361 ttcagaataa aaatatttga attcaaaatg caacgatcgc cccgaaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatc atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagttcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag ttcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt aaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg tttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatagctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcctgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caatcatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gctgcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaaatt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgaca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgtgtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattac gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg tgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 tgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    35281 ggcggcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca cctgccattg
    35341 cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg ccaaccattc
    35401 caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta
    35461 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    35521 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    35581 tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg aaaaccgagc
    35641 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa atgctgccag
    35701 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    35761 cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    35821 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg actgttctcg
    35881 acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga
    35941 cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    36001 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    36061 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    36121 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    36181 tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact tcatgcgcca
    36241 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    36301 gtgtaccgct gccccatacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc
    36361 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    36421 acaggttggt cggcatgaca gagcggtaat cgcgtccata ctggcggttg taagattcgc
    36481 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    36541 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    36601 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    36661 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    36721 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    36781 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    36841 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    36901 aaatgcgttg tttacttatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    36961 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    37021 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    37081 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    37141 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    37201 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    37261 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    37321 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    37381 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    37441 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    37501 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    37561 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    37621 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    37681 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    37741 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    37801 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    37861 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    37921 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    37981 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    38041 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    38101 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    38161 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    38221 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    38281 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    38341 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    38401 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    38461 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    38521 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    38581 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    38641 tgttatataa ggctttagtc ttacctgtcg tacgttggcg taaatgccag gataattctg
    38701 cccagaagcc aggtacatgt aatatctgcc gttggacttt tttggcgtca gcaca
//
LOCUS       DAJTRQ010000020        37308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000020 DAJTRQ010000000
VERSION     DAJTRQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(130..1014)
                     /gene="yhdJ"
                     /locus_tag="OO332_001194"
     CDS             complement(130..1014)
                     /gene="yhdJ"
                     /locus_tag="OO332_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5941750.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1100..1396)
                     /gene="fis"
                     /locus_tag="OO332_001195"
     CDS             complement(1100..1396)
                     /gene="fis"
                     /locus_tag="OO332_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5941751.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1422..2387)
                     /gene="dusB"
                     /locus_tag="OO332_001196"
     CDS             complement(1422..2387)
                     /gene="dusB"
                     /locus_tag="OO332_001196"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5941752.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2716..3597)
                     /gene="prmA"
                     /locus_tag="OO332_001197"
     CDS             complement(2716..3597)
                     /gene="prmA"
                     /locus_tag="OO332_001197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5941753.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3609..5060)
                     /gene="panF"
                     /locus_tag="OO332_001198"
     CDS             complement(3609..5060)
                     /gene="panF"
                     /locus_tag="OO332_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5941754.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5050..5292)
                     /locus_tag="OO332_001199"
     CDS             complement(5050..5292)
                     /locus_tag="OO332_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5941755.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5401..6750)
                     /gene="accC"
                     /locus_tag="OO332_001200"
     CDS             complement(5401..6750)
                     /gene="accC"
                     /locus_tag="OO332_001200"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5941756.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6761..7231)
                     /gene="accB"
                     /locus_tag="OO332_001201"
                     /gene_synonym="fabE"
     CDS             complement(6761..7231)
                     /gene="accB"
                     /locus_tag="OO332_001201"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5941757.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8209..9183)
                     /locus_tag="OO332_001202"
     CDS             complement(8209..9183)
                     /locus_tag="OO332_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5941758.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9335..11275
                     /gene="csrD"
                     /locus_tag="OO332_001203"
     CDS             9335..11275
                     /gene="csrD"
                     /locus_tag="OO332_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5941759.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11580..12623
                     /gene="mreB"
                     /locus_tag="OO332_001204"
     CDS             11580..12623
                     /gene="mreB"
                     /locus_tag="OO332_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5941760.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12689..13792
                     /gene="mreC"
                     /locus_tag="OO332_001205"
     CDS             12689..13792
                     /gene="mreC"
                     /locus_tag="OO332_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5941761.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13792..14280
                     /gene="mreD"
                     /locus_tag="OO332_001206"
     CDS             13792..14280
                     /gene="mreD"
                     /locus_tag="OO332_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5941762.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14289..14882
                     /gene="yhdE"
                     /locus_tag="OO332_001207"
     CDS             14289..14882
                     /gene="yhdE"
                     /locus_tag="OO332_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5941763.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14872..16341
                     /gene="rng"
                     /locus_tag="OO332_001208"
     CDS             14872..16341
                     /gene="rng"
                     /locus_tag="OO332_001208"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5941764.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16409..20209
                     /gene="yhdP"
                     /locus_tag="OO332_001209"
     CDS             16409..20209
                     /gene="yhdP"
                     /locus_tag="OO332_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5941765.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20365..21810
                     /gene="tldD"
                     /locus_tag="OO332_001210"
     CDS             20365..21810
                     /gene="tldD"
                     /locus_tag="OO332_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5941766.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21938..22867)
                     /gene="aaeR"
                     /locus_tag="OO332_001211"
     CDS             complement(21938..22867)
                     /gene="aaeR"
                     /locus_tag="OO332_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5941767.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23050..23253
                     /locus_tag="OO332_001212"
     CDS             23050..23253
                     /locus_tag="OO332_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5941768.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23261..24193
                     /gene="aaeA"
                     /locus_tag="OO332_001213"
     CDS             23261..24193
                     /gene="aaeA"
                     /locus_tag="OO332_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5941769.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24199..26166
                     /gene="aaeB"
                     /locus_tag="OO332_001214"
     CDS             24199..26166
                     /gene="aaeB"
                     /locus_tag="OO332_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5941770.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26258..26530
                     /locus_tag="OO332_001215"
     CDS             26258..26530
                     /locus_tag="OO332_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5941771.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26586..26849)
                     /gene="yhcN"
                     /locus_tag="OO332_001216"
     CDS             complement(26586..26849)
                     /gene="yhcN"
                     /locus_tag="OO332_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5941772.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27214..27684)
                     /gene="argR"
                     /locus_tag="OO332_001217"
     CDS             complement(27214..27684)
                     /gene="argR"
                     /locus_tag="OO332_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5941773.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28119..29057
                     /gene="mdh"
                     /locus_tag="OO332_001218"
     CDS             28119..29057
                     /gene="mdh"
                     /locus_tag="OO332_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5941774.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29119..30186)
                     /gene="degS"
                     /locus_tag="OO332_001219"
     CDS             complement(29119..30186)
                     /gene="degS"
                     /locus_tag="OO332_001219"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5941775.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30276..31643)
                     /gene="degQ"
                     /locus_tag="OO332_001220"
     CDS             complement(30276..31643)
                     /gene="degQ"
                     /locus_tag="OO332_001220"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5941776.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31797..32195)
                     /locus_tag="OO332_001221"
     CDS             complement(31797..32195)
                     /locus_tag="OO332_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5941777.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32389..33516
                     /gene="zapE"
                     /locus_tag="OO332_001222"
     CDS             32389..33516
                     /gene="zapE"
                     /locus_tag="OO332_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5941778.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33735..34163
                     /gene="rplM"
                     /locus_tag="OO332_001223"
     CDS             33735..34163
                     /gene="rplM"
                     /locus_tag="OO332_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5941779.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34179..34571
                     /gene="rpsI"
                     /locus_tag="OO332_001224"
     CDS             34179..34571
                     /gene="rpsI"
                     /locus_tag="OO332_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5941780.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34966..35604
                     /gene="sspA"
                     /locus_tag="OO332_001225"
     CDS             34966..35604
                     /gene="sspA"
                     /locus_tag="OO332_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5941781.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35610..36107
                     /gene="sspB"
                     /locus_tag="OO332_001226"
     CDS             35610..36107
                     /gene="sspB"
                     /locus_tag="OO332_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5941782.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36150..>37308)
                     /gene="dcuC"
                     /locus_tag="OO332_001227"
     CDS             complement(36150..>37308)
                     /gene="dcuC"
                     /locus_tag="OO332_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5941783.1"
                     /translation="NRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPY
                     IILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWG
                     ILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQ
                     AEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVE
                     FFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHS
                     GLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFAT
                     TLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9016 a         9336 c         9836 g         9120 t
ORIGIN      
        1 aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc tgagcctgtg
       61 catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa aaccaggctt
      121 acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc ttcgttttga
      181 caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt aatgcgacgc
      241 gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa taccaatgaa
      301 ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag
      361 aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg ctttcggttt
      421 ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgtggaa aatcccagac
      481 gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat aatagatcaa
      541 cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga atgtgtagtt
      601 ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt gttttttcgc
      661 ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa aaagcttgcg
      721 gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca tgctgccctg
      781 ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga taaacagatc
      841 ttctttccag gcttcgatca aaccatcaaa atttttaccg atgttatacg gtgggtcggc
      901 aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaggg catcaccgtg
      961 aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc tcatggtcac
     1021 tccctttgtg acacctataa aaaaggcgct tccctatgcc gagtagcgcc tttttaatca
     1081 agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta cgcagcgtac
     1141 cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg tattgcatca
     1201 ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg tcattcacat
     1261 cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag tcacgcaggg
     1321 gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg tcagaattta
     1381 cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa attttcgaag
     1441 tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga
     1501 aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg
     1561 tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc
     1621 ttaacctctg ccaaaggcag cgggggcagc aactccccag tgtccagata atgctggatt
     1681 tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc atccgcccct
     1741 gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc
     1801 ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc tccattgaac
     1861 aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc ttcagccagt
     1921 tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat cttcagggta
     1981 acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac atccgggtac
     2041 tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca acccatattg
     2101 atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc catttctttc
     2161 ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg caccatccgt
     2221 aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc ggatactgtc
     2281 aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg
     2341 ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag ttaagaaatg
     2401 accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca
     2461 aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag cgtggtgcga
     2521 taattactca taaaaatcat catattagaa aatagtgact aaaatttaca ctcaaagaaa
     2581 tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa atttgcgtaa
     2641 ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct
     2701 acggcgatgc gaaggttaat tcttacgacc ggtaatacgg caccactctt ctttttccac
     2761 gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg cctggcttgc
     2821 cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc
     2881 cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt tcatttcttc
     2941 tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac gttcggcgtt
     3001 atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt ttgctgcacc
     3061 cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga tgactgtttt
     3121 accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg gatgggtacc
     3181 cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt ccggcacatc
     3241 acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga aattatccat
     3301 ccattcgcgc tcccagtctt tatcttctag ttgttcggtt ttatgcgcga agcctgcgcc
     3361 gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa
     3421 cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg gctcaaatac
     3481 tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca tcagcgcatc
     3541 gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc aaggcatggc
     3601 aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg
     3661 tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg gtggaagccc
     3721 aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac gatcatcgca
     3781 cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag cggccacagg
     3841 aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat ttctggcggc
     3901 ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc cgacatccgc
     3961 ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag atctttaatg
     4021 atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc agccataggt
     4081 gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa cgtcgggatc
     4141 accagatccg gtacggtgag atcggggatc actgcacgac ctaacgctcc ggctaggtgc
     4201 ataccgaaca tcaggattgc gactacaatc gtaccgatga tgatcccccg gtgtactgct
     4261 ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat cacgccaaag
     4321 cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt
     4381 ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct taagccgcca
     4441 gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac aagcccttgc
     4501 atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa cgcaatgctg
     4561 ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc
     4621 gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa actcaaactc
     4681 gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt cagcgtcact
     4741 gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag tgaaagccag
     4801 acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt ataagcagct
     4861 cctggcccgc cgataaacga actggcactg atataggtcg cggtgagcgt catcgccagc
     4921 acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt gccggtgctc
     4981 cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc gaccagcggt
     5041 agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga taaatttcac
     5101 catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca tctcaaacca
     5161 gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcatcta ctaaccaaac
     5221 tgccagatac aaaagggtca gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa
     5281 actagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac cttatggggg
     5341 ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc
     5401 ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc caccatgctg
     5461 gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga taccgtcgat
     5521 gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta
     5581 gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc ccgcgtagat
     5641 atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg
     5701 gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg catggccgcg
     5761 aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac gcagctgttc
     5821 tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct gaatacgggt
     5881 gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag tacctgcacc
     5941 gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc gacgcagttc
     6001 cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc gctgcatgga
     6061 gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta cctgaatctc
     6121 gatgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc tgaaagcagc
     6181 tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac
     6241 gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat aaccaatgcg
     6301 tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag aaccaggtac
     6361 gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc ccatcaggcg
     6421 aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct gctcggcaaa
     6481 gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc cggtgatttc
     6541 agcggcgctg atgattgccg ggatgttcag ataactttgt actgacggag cagggccaat
     6601 acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac
     6661 agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg caatctcgcc
     6721 gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg acgaccagcg
     6781 gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg gtaccggatt
     6841 tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg gtatcgccca
     6901 cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga
     6961 aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct gctggcgctt
     7021 ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc tgcatcattg
     7081 gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca cggctaatgc
     7141 gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct tcaaccagct
     7201 cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt
     7261 gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata
     7321 gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt caatgttaga
     7381 cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa aatatacctt
     7441 cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc
     7501 acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag ggaagataat
     7561 caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc
     7621 acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa gctgaaaagc
     7681 cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca aataatgtag
     7741 ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg gattacccaa
     7801 ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca accaactgac
     7861 aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa tcagattatt
     7921 caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc
     7981 ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag tatcaatgcg
     8041 catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt gtacaacttc
     8101 ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta accgattgaa
     8161 taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt agttaacctt
     8221 caccagcgtg cgaccttgga tctggttatt aatgatggcc tcggcaaagt tcggtgcctc
     8281 tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat cggcgaccag
     8341 tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa
     8401 acggacatta cgcagaataa atggcatgac cgtgcttggc agagtaaaac cacccgccag
     8461 accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg ccaggacttt
     8521 gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac gggattcggc
     8581 aaactcatca cgagggagaa tacggctggc acctaaactt ttcagatatt catgggtact
     8641 ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg ccacggcggt
     8701 actgccgacg ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc
     8761 ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac cgataatcat
     8821 tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc
     8881 ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga gtaacacctc
     8941 ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc
     9001 aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt
     9061 atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca ggcgactttc
     9121 gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg
     9181 cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac tatactcgct
     9241 aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga tttgctagta
     9301 tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga aattttcggc
     9361 ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct gttcgctaag
     9421 tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg cgacggcgat
     9481 tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta ccgaattaat
     9541 ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg tttataccct
     9601 ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg aactgagcgt
     9661 tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc cgatgggcaa
     9721 ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct ttatcattct
     9781 tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag aattgctgga
     9841 aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg gaaccatcta
     9901 tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa ttcagaacgc
     9961 acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac aggacgtgaa
    10021 aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac tggaagatca
    10081 ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt
    10141 gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga cgaatctgct
    10201 gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc gcagtgattt
    10261 tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca gtcagttaat
    10321 caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata tgatccacat
    10381 tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac atgcagagtc
    10441 tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg atgactcgtt
    10501 gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg
    10561 cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg
    10621 cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc
    10681 gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat
    10741 tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg ttgagtcgct
    10801 gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca
    10861 acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata tcagtcgttt
    10921 acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca accaggctgg
    10981 tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac tcaagctcca
    11041 tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg ttcaaagcct
    11101 ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag
    11161 cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc
    11221 ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg tttaacctgc
    11281 cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct
    11341 gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt gcaaacaggc
    11401 gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg taacaaagga
    11461 aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg ctgctgcgtg
    11521 tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta tcccttagta
    11581 tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg ggtactgcga
    11641 ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc gtggtggcca
    11701 ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc
    11761 agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa gacggcgtta
    11821 tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa gtgcacagca
    11881 acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc gcgacccagg
    11941 ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga
    12001 ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt
    12061 ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg
    12121 tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct atcatcaact
    12181 atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt atcaagcacg
    12241 aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc
    12301 tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc gaagcactgc
    12361 aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag tgcccgccgg
    12421 aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc gcactgctgc
    12481 gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt gctgaagacc
    12541 cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac atgcacggcg
    12601 gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt ttaccctgcc
    12661 tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg gcccgtcgct
    12721 acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg ccgacagccg
    12781 cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc ctttctactt
    12841 tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg cctcgcgtga
    12901 ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa acagtgaact
    12961 gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc
    13021 gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta acgatcctta
    13081 tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag gccagccggt
    13141 catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt
    13201 gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca acgatatccg
    13261 cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc tgccagcgaa
    13321 tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc gtttcccgga
    13381 aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg cttatactgt
    13441 gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc tgctgtgggg
    13501 cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg ttgctaatga
    13561 acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt
    13621 acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga caggaaatgc
    13681 agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac aaagggctac
    13741 gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc
    13801 tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa
    13861 atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt acttatcttg
    13921 ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt tgtgatgggt
    13981 gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc gatgagcatc
    14041 attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt atggcagcag
    14101 gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg ggcagagttt
    14161 ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt caatggggtg
    14221 ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc cgtgcaataa
    14281 aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag gagttacttg
    14341 cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag cgtcagccgc
    14401 aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag gcgggtgtcg
    14461 cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc ctgaacggag
    14521 aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc aaattatcgg
    14581 gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcat attctcgatt
    14641 gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc gcgggctatg
    14701 tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg ctgggtggct
    14761 gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg ctggttgaaa
    14821 cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa catgacggct
    14881 gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga tggcggtatt
    14941 ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag
    15001 ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat tgggctggat
    15061 aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt ggcgggtgaa
    15121 gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg
    15181 atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac caccgatatc
    15241 acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg ggtttcccaa
    15301 cgaattgaaa gcgaatcaga acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac
    15361 gagcagggcg ggtttatcat ccgcactgca gcggaagggg ttggcgaggc tgaactggcc
    15421 tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg
    15481 cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct gcgtgatttt
    15541 gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga agcattgctg
    15601 gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta caccggacgc
    15661 cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt ggaacgcaaa
    15721 gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat gaccaccgtg
    15781 gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat tttcaatacc
    15841 aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct gggcgggatt
    15901 atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt gctgcattcg
    15961 ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg
    16021 gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac
    16081 gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa
    16141 atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt cctggtctat
    16201 gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct ggcggaagtg
    16261 gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa ccaggagcag
    16321 tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg gtttttgagt
    16381 cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt tactgcttac
    16441 tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta ttgctttacc
    16501 gcatcttgac gcctggcgtc cggaaatcct caacaaaata gaatccgcga ctggcatgcc
    16561 ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc ttgaagcaca
    16621 cgatatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg ttactctggc
    16681 gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc tcactttctg
    16741 gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg acagtctgga
    16801 agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc tccgcgacag
    16861 tgaagtcagt tttctgacgc catccggtca gagcgccgag ctggcgatcc cacaactcac
    16921 ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct ccagccttac
    16981 cgggcagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag
    17041 caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc tcggtaaatg
    17101 gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct ggatgacgat
    17161 cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt
    17221 gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata ttacgcgtga
    17281 aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg gcaaaccctg
    17341 gccgagcgga gcattgacgc tggcctggat accggaacag gacgttgacg gcaaagacaa
    17401 taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag gcctggaggg
    17461 catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc gctccacaca
    17521 accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg cagacaagac
    17581 ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat taccgggtgc
    17641 ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat
    17701 gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa tcgccgacgg
    17761 ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg ggcgtaatat
    17821 tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc aacctgctaa
    17881 cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt cacaagcctg
    17941 gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa gtggtgcgat
    18001 tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc aactcttccc
    18061 ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc
    18121 cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact ttattaacga
    18181 cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac
    18241 cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca ttaaaggtcc
    18301 gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc tgggtgcgac
    18361 cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg atatcccgct
    18421 gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca gtctgtttat
    18481 caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga
    18541 tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg tggatttttc
    18601 caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta actggcaacc
    18661 ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg
    18721 ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata acgtagagct
    18781 gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca aacctgcggg
    18841 tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg aattaaccgg
    18901 acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa agctgacgct
    18961 cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg
    19021 cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg
    19081 cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacat tacgtacgcc
    19141 tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc aaccgacggc
    19201 aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa
    19261 taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg tggcgaaagc
    19321 tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta acttccgtgg
    19381 ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa aattcggtcg
    19441 cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg gactgattga
    19501 tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg
    19561 tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag aattttttgg
    19621 tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac actggcgcaa
    19681 agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc aactgggtaa
    19741 aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct tattgagcgt
    19801 agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg aagggttcta
    19861 ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca ccgacgacac
    19921 gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg
    19981 agacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc
    20041 ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta aagtgctggg
    20101 gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc tggatgatcc
    20161 gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg
    20221 cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag ccggaacata
    20281 actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat cctctactgc
    20341 agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca attgctagcg
    20401 gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc
    20461 cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt tttagaagac
    20521 cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc
    20581 ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct ggaacagagt
    20641 gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc
    20701 gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa
    20761 gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta
    20821 caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc ggccaccgac
    20881 ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa
    20941 gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttag ttatgagttc
    21001 ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt
    21061 atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt
    21121 ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct ggaaggcgac
    21181 ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt ggcttcagaa
    21241 ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat
    21301 gacgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct gaaaggctac
    21361 atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt
    21421 gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct gccgggtaaa
    21481 tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc gaactttggt
    21541 ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga agcatatctg
    21601 attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc
    21661 gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa cggcgtgggt
    21721 gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac gttgaaagtc
    21781 gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa ggataattaa
    21841 tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag gggaacccgt
    21901 tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc ccgcgcccgt
    21961 gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag ttgatcacga
    22021 cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc gggcgtggat
    22081 ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg ttgatcaccc
    22141 acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc agcgtcatcg
    22201 gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct tccggtgcaa
    22261 tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat
    22321 cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg cacaccacca
    22381 ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga
    22441 tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta accagattgg
    22501 cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga acattttgtg
    22561 ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg aaggcataca
    22621 gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg tagtaaattc
    22681 taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt acctgcaact
    22741 catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt agctgtctgg
    22801 cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg cgttttagtc
    22861 gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga taacagcata
    22921 acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc ccggctttcc
    22981 atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct ctatacaacc
    23041 aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt cccaccgata
    23101 ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt acttgtgcca
    23161 acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct ctattgctgc
    23221 ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac taataagaaa
    23281 attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg caatttttaa
    23341 tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt
    23401 cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc atgataacca
    23461 gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc aaaaggcgct
    23521 tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga aacgccagga
    23581 ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc
    23641 taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct
    23701 ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct gggtgaccaa
    23761 cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg cgctggtgaa
    23821 acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag gggtgcgtcc
    23881 ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga
    23941 tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag ggatggcgac
    24001 catcgactct aaccttgaat gggtgcgttt ggcgcaacgt gttccggttc gtattcgtct
    24061 cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg tggtcactgg
    24121 caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc atcgcctgcg
    24181 tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat tcgctttgcg
    24241 gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt ccagctggaa
    24301 acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc ctttgctgcg
    24361 ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat catcggcaca
    24421 tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc accattattg
    24481 atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc gctggtacga
    24541 atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat tgtgatcacc
    24601 attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt
    24661 atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc gatcaaacaa
    24721 gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat gcaactctgt
    24781 atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt gcgacgcacc
    24841 acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg ggcgcgggcc
    24901 aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca atcctgcgaa
    24961 acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg cgaatttttt
    25021 gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt
    25081 atcgcctgga ccggggaacg ggaaacgcct gtcaccattt atagctgggt cgcggcggca
    25141 acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa
    25201 gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg tcatcatgca
    25261 atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt ctggctgtgg
    25321 acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac gtcactggca
    25381 atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac gctggccgcg
    25441 ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca gagcatgttg
    25501 ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt acagaaacgg
    25561 cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct ggataacccg
    25621 atgactttcc atttcagtca gtttctcgac agcgcattag ggcaaatcgt cggctgtgtg
    25681 ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac tggacgtgta
    25741 ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt
    25801 aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa taagttccca
    25861 ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt
    25921 gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat gcgccgcaca
    25981 gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt tggccagttg
    26041 ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg
    26101 acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc gttgaccgat
    26161 agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact tagcgatgca
    26221 cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg cctttgattt
    26281 taatgagatt gagagtcagg aggattttta tcgtgacttt agccaaaccc ttggtctggc
    26341 taaagataag gtacgcgatc ttgactcact atgggatgtg ttaatgaacg atgtcctgcc
    26401 gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc
    26461 gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc gttttaatgt
    26521 tcgtcattag cgcaaaaaaa agcccccgaa ccgggggcaa tatcgtcgga caaaacgatg
    26581 agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg agcttcagta
    26641 atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc acgtatatcc
    26701 attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg attttgtgct
    26761 tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga aagtacgctt
    26821 aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc ttttagctgg
    26881 gcctttgttt cgtgacccat atcacaaaat caagcataca ctaattattg aactaattaa
    26941 taccacgcta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt aataacaaaa
    27001 aagtataaaa aacacggtta tttattatta attatagtat tttcaattag ataatgaatt
    27061 ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg tcgcggtaaa
    27121 acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg ataagcaaca
    27181 ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga acagctctaa
    27241 aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa agatggtgtc
    27301 atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca gcaggcgagc
    27361 aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt tgtagtcgat
    27421 atccagcacc agattcttca atggactgga ggtggttggt acacccagtt cagctggcag
    27481 gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact tggtcaacat
    27541 ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg cggcgacgat
    27601 ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt taactagttc
    27661 ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac aacattattg
    27721 tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa ataatgttat
    27781 gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca
    27841 aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc tgaacggtag
    27901 ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg ttaattacgt
    27961 aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg tgactacaca
    28021 ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg gcagcggagc
    28081 aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca gtcctcggcg
    28141 ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg ccttcaggtt
    28201 cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc gatctgagcc
    28261 atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact ccggcgctgg
    28321 aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt atggatcgtt
    28381 ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa
    28441 actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca gttgcaattg
    28501 ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc ggcgttacca
    28561 cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa cagccaggcg
    28621 aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg ctgctgtcac
    28681 aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa cgtatccaga
    28741 acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg
    28801 gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg
    28861 ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc tctcaaccgc
    28921 tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg agcgcatttg
    28981 aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc ctgggcgaag
    29041 agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag actccggttt
    29101 ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga cctgcagcgt
    29161 taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc gaatttccgc
    29221 cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg aaataatcag
    29281 atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt cattaaccac
    29341 gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga tctcgcgtcc
    29401 gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat ccataatttt
    29461 ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc catcgttact
    29521 cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca ccagcgcgcc
    29581 gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc gcccggtcgg
    29641 gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct gcccgaggtt
    29701 gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac gtgcattaat
    29761 tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat cggttagaga
    29821 gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga cgatgatctg
    29881 atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat ccatgattac
    29941 accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca aaccacggtt
    30001 gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc tggcaggcgt
    30061 ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca gcgaaggcat
    30121 ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta agagcttcac
    30181 aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg agttaaccgg
    30241 acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag atagatgctt
    30301 tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag cactttacgc
    30361 atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc gtctttttgc
    30421 aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat acctttgccg
    30481 ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggcgt gatcatttca
    30541 gcgctggccg acgaagaggt gttggtatca agcgtcactt ctacttccag tggtttgccg
    30601 ttacgcagca ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc
    30661 aactcagcaa agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg
    30721 acgcccgctt tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc
    30781 acgtcaaggt tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac
    30841 aaaccgcgtt tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta
    30901 ctggggatgg caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg
    30961 atgccaatta actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg
    31021 gaagcatctg tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat
    31081 gcggaaacaa tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg
    31141 gcaaaatcac cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc
    31201 gggttttgaa tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagtttttca
    31261 tcaaactcgc gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg
    31321 ttgttggtca gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg
    31381 aagggttgtg caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg
    31441 attttctgtc cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact
    31501 ttttccagca ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc
    31561 gacgcgacgg cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc
    31621 aacagctggg tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga
    31681 ttcaaaacag tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa
    31741 tgatagtcgt ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag
    31801 tcgcgcttcg cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcatc
    31861 tgcaccggtg cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt
    31921 gcttcagcag acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc
    31981 tggcgatagt cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc
    32041 agctcttcgc gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc
    32101 tgttgctggc gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg
    32161 acgactaacc caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt
    32221 ccgtagggtg ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa
    32281 ttccacagaa gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc
    32341 aaccgtgaac aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt
    32401 accccaacat cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt
    32461 caaaaagagg ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca
    32521 ccagccccca ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa
    32581 gacacaaagc atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa
    32641 acctggctga tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac
    32701 tttcaccgtt ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat
    32761 ccgctggaaa ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa
    32821 ttttttgttt ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc
    32881 acccgcggta ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat
    32941 ggcctgcaac gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta
    33001 atgaacgtgg acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg
    33061 ctttcgccac tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg
    33121 ggggtgaaac gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg
    33181 ggcgtcgaga accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt
    33241 cagcatgact atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca
    33301 gtcatgacgc ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt
    33361 tacgagcgcc atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag
    33421 ggtgagcggc tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc
    33481 gaagagtatc tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg
    33541 ggtcgatctt tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt
    33601 tttcccaaaa ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt
    33661 tgtcgtgtga acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat
    33721 tgggtaagct tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt
    33781 atgttgttga cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc
    33841 tgcgtggtaa gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg
    33901 ttctgaacgc tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc
    33961 accacaccgg ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc
    34021 gtcctgagcg tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc
    34081 gtgctatgtt ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc
    34141 aaccgcaagt tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg
    34201 gcactggtcg ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa
    34261 tcgtaatcaa ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag
    34321 ttcgtcagcc gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta
    34381 aaggtggtgg tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga
    34441 tggaatacga cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg
    34501 ctcgtcaggt tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct
    34561 ccaaacgtta attggcttct gctccggcag aaaacaattt gcgaaaaaac ccgcttcggc
    34621 gggttttttt atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc
    34681 cgtctgaaag acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt
    34741 cagactaaag cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa
    34801 aatctggtaa actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt
    34861 tgattttcga acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct
    34921 ggtggctggt agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc
    34981 aacaaacgtt cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc
    35041 cgcattgtgc tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat
    35101 ccgcctcagg atctgattga ccttaacccg aatcagagcg ttccgaccct ggtggatcgt
    35161 gagctgaccc tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat
    35221 ccgccactga tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc
    35281 atcgaaaaag actggtacac gctgataaac actatcatca acggttcagc ttctgaagca
    35341 gatgccgcac gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag
    35401 aagccgtact tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg
    35461 tggcgtctgc cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc
    35521 tatatgaccc gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt
    35581 gaaatgcgtc tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc
    35641 cctatctgct gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacttgg
    35701 tggtggatgt gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa
    35761 tcgtactcaa cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc
    35821 gctttaacgc gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc
    35881 tggctatcta cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg
    35941 atgaagatac cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt
    36001 cggttattga tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc
    36061 cgcagccacc acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc
    36121 caggcggcct gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata
    36181 ttcaccacga aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa
    36241 acgcctgcaa ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct
    36301 agcgtcgtgg caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct
    36361 gcgatattcg gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca
    36421 atggcacaaa tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca
    36481 gaatgctccg cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca
    36541 aatatttcac ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca
    36601 aaaaacgcct gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca
    36661 aacatcgtca ccgtcaaact tagtaacatc accaccacca gatgcagttc tgacttcatc
    36721 agcccgacgt gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa
    36781 atggcgtaat agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg
    36841 atatttttat cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag
    36901 ataatgacgc aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt
    36961 cccgcgacct gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc
    37021 gtggttgcga taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga
    37081 aataaggtga ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg
    37141 atttggccga tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac
    37201 gggcggctta gcaagctcac catcgcgcga ctggctccta tgcgctccat gtagcgggca
    37261 taaccgccca ccgccataat cgacagcccc aatccggcaa tgcggttg
//
LOCUS       DAJTRQ010000021        36634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000021 DAJTRQ010000000
VERSION     DAJTRQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(156..1391)
                     /locus_tag="OO332_001228"
     CDS             complement(156..1391)
                     /locus_tag="OO332_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5941784.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO332_001229"
     tRNA            complement(1538..1614)
                     /locus_tag="OO332_001229"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO332_001230"
     tRNA            complement(1657..1743)
                     /locus_tag="OO332_001230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO332_001231"
     tRNA            complement(1764..1839)
                     /locus_tag="OO332_001231"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO332_001232"
     tRNA            complement(1898..1974)
                     /locus_tag="OO332_001232"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO332_001233"
     CDS             complement(2077..3462)
                     /locus_tag="OO332_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5941785.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO332_001234"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO332_001234"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5941786.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO332_001235"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO332_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5941787.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO332_001236"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO332_001236"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5941788.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO332_001237"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO332_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5941789.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO332_001238"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO332_001238"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5941790.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO332_001239"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO332_001239"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5941791.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO332_001240"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO332_001240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5941792.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO332_001241"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO332_001241"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5941793.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO332_001242"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO332_001242"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5941794.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO332_001243"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO332_001243"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5941795.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO332_001244"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO332_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5941796.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO332_001245"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO332_001245"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5941797.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO332_001246"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO332_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5941798.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO332_001247"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO332_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5941799.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO332_001248"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO332_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5941800.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO332_001249"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO332_001249"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5941801.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO332_001250"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO332_001250"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5941802.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO332_001251"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO332_001251"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5941803.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO332_001252"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO332_001252"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5941804.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO332_001253"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO332_001253"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5941805.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO332_001254"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO332_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5941806.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO332_001255"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO332_001255"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5941807.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO332_001256"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO332_001256"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5941808.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO332_001257"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO332_001257"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5941809.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO332_001258"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO332_001258"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5941810.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO332_001259"
     CDS             complement(31267..32913)
                     /locus_tag="OO332_001259"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5941811.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO332_001260"
     CDS             complement(32916..32966)
                     /locus_tag="OO332_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941812.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO332_001261"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO332_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5941813.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="OO332_001262"
     CDS             33502..35022
                     /locus_tag="OO332_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5941814.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO332_001263"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO332_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5941815.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="OO332_001264"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="OO332_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5941816.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="OO332_001265"
     tRNA            complement(36439..36514)
                     /locus_tag="OO332_001265"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
     gene            complement(36523..36599)
                     /locus_tag="OO332_001266"
     tRNA            complement(36523..36599)
                     /locus_tag="OO332_001266"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36563..36565),aa:Asp,seq:gtc)
BASE COUNT         8946 a        10030 c         9229 g         8429 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccaacccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgccaaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcaac tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag tcaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattca gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaattta ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctctcga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttaccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcatgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgggc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatggttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca cgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattct ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtggtgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggagcacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggct attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgct ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggag tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcata gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgttcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgctat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa cggacgggac
    36541 tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctcca
    36601 ccgaattctt ttacaaccac cggttttatg accg
//
LOCUS       DAJTRQ010000022        36495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000022 DAJTRQ010000000
VERSION     DAJTRQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="OO332_001267"
     CDS             complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="OO332_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5941817.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
     gene            complement(308..1021)
                     /locus_tag="OO332_001268"
     CDS             complement(308..1021)
                     /locus_tag="OO332_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5941818.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="OO332_001269"
     CDS             complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="OO332_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5941819.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2464..2994
                     /gene="rppH"
                     /locus_tag="OO332_001270"
     CDS             2464..2994
                     /gene="rppH"
                     /locus_tag="OO332_001270"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5941820.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            3007..5253
                     /gene="ptsP"
                     /locus_tag="OO332_001271"
     CDS             3007..5253
                     /gene="ptsP"
                     /locus_tag="OO332_001271"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5941821.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5404..6279
                     /gene="lgt"
                     /locus_tag="OO332_001272"
     CDS             5404..6279
                     /gene="lgt"
                     /locus_tag="OO332_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5941822.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6286..7080
                     /gene="thyA"
                     /locus_tag="OO332_001273"
     CDS             6286..7080
                     /gene="thyA"
                     /locus_tag="OO332_001273"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5941823.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7264..7734
                     /locus_tag="OO332_001274"
                     /pseudo
     CDS             7264..7734
                     /locus_tag="OO332_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7725..8288
                     /locus_tag="OO332_001275"
     CDS             7725..8288
                     /locus_tag="OO332_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5941824.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8285..8692
                     /locus_tag="OO332_001276"
     CDS             8285..8692
                     /locus_tag="OO332_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5941825.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8677..9000
                     /locus_tag="OO332_001277"
     CDS             8677..9000
                     /locus_tag="OO332_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5941826.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            9013..12381
                     /gene="recC"
                     /locus_tag="OO332_001278"
     CDS             9013..12381
                     /gene="recC"
                     /locus_tag="OO332_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5941827.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12557..15445
                     /gene="ptrA"
                     /locus_tag="OO332_001279"
     CDS             12557..15445
                     /gene="ptrA"
                     /locus_tag="OO332_001279"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5941828.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15438..18980
                     /gene="recB"
                     /locus_tag="OO332_001280"
     CDS             15438..18980
                     /gene="recB"
                     /locus_tag="OO332_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5941829.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18980..20806
                     /gene="recD"
                     /locus_tag="OO332_001281"
     CDS             18980..20806
                     /gene="recD"
                     /locus_tag="OO332_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5941830.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20868..22199)
                     /gene="argA"
                     /locus_tag="OO332_001282"
     CDS             complement(20868..22199)
                     /gene="argA"
                     /locus_tag="OO332_001282"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5941831.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22431..23684
                     /gene="amiC"
                     /locus_tag="OO332_001283"
     CDS             22431..23684
                     /gene="amiC"
                     /locus_tag="OO332_001283"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5941832.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23759..23835)
                     /locus_tag="OO332_001284"
     tRNA            complement(23759..23835)
                     /locus_tag="OO332_001284"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23799..23801),aa:Met,seq:cat)
     gene            24044..25141
                     /gene="mltA"
                     /locus_tag="OO332_001285"
     CDS             24044..25141
                     /gene="mltA"
                     /locus_tag="OO332_001285"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5941833.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25380..26186
                     /gene="tcdA"
                     /locus_tag="OO332_001286"
     CDS             25380..26186
                     /gene="tcdA"
                     /locus_tag="OO332_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5941834.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="OO332_001287"
     CDS             complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="OO332_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5941835.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="OO332_001288"
     CDS             complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="OO332_001288"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5941836.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28077..28304
                     /locus_tag="OO332_001289"
     CDS             28077..28304
                     /locus_tag="OO332_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5941837.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28655..29572
                     /gene="gcvA"
                     /locus_tag="OO332_001290"
     CDS             28655..29572
                     /gene="gcvA"
                     /locus_tag="OO332_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5941838.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29591..29986
                     /locus_tag="OO332_001291"
     CDS             29591..29986
                     /locus_tag="OO332_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5941839.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            29979..31079
                     /gene="rlmM"
                     /locus_tag="OO332_001292"
     CDS             29979..31079
                     /gene="rlmM"
                     /locus_tag="OO332_001292"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5941840.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="OO332_001293"
     CDS             complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="OO332_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5941841.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="OO332_001294"
     CDS             complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="OO332_001294"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5941842.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="OO332_001295"
                     /pseudo
     CDS             complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="OO332_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="OO332_001296"
     CDS             complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="OO332_001296"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5941843.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35672..>36495)
                     /gene="fucP"
                     /locus_tag="OO332_001297"
     CDS             complement(35672..>36495)
                     /gene="fucP"
                     /locus_tag="OO332_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5941844.1"
                     /translation="AIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQ
                     TPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQ
                     FCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPH
                     KVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYG
                     SSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8682 a         9104 c         9894 g         8815 t
ORIGIN      
        1 ttaccttgct gatcgtgata actcaccagc ccggtatcat catcaatttc aggttttcca
       61 tcggtcaaaa tcatacggcc atctttggtc gccatgacgt aatcactgga acacccggaa
      121 acagcaaacg ccagtccgac tgcagaaatt attactgccc attttttcat ccggttatcc
      181 tcatttggcc tacctatgat aatagtctgt ttataaatcg acgttatata tcttgaaagc
      241 aggaaaatct taaactgtga gggtaaaagg agtgagtacg ccctcccgca ggagagcgta
      301 cggagtatca gagcggattc tttttgttgc gaatcaagtt gaggctttca accgcaatag
      361 agaagaacat cgcgaagtag atgtaacctt tcggtacgtg gatgtcgaaa ctttccagaa
      421 tcagggtaaa gcccaccaga atcaggaaag agagcgccag catttttacc gaaggatggc
      481 gttcgacaaa atcaccaatc gagcgcgcgg cgaacatcat cacgcctacg gcaatcacca
      541 cggctgccat cataataaac aggtgatctg acagacccac agcggtaatc accgagtcga
      601 ggctgaagat aatatccagc agcataatct gcacgatagc gccgaggaat gatgaaacgc
      661 gtgttttcag cccttcttct tcaccttcaa tggattcgtg gatttccttg ctggctttcc
      721 agataaggaa caagccaccc agaagcagaa tcaaatcacg ggcagaaatt tcctgactga
      781 atattgtaaa aagcggattt gtcaggcgcg taacccaaga gattgatgcc agcagagcca
      841 gacgcataac catggctccc gccaacccca gacggcgcgc atgagcacgt tgtgctgtgg
      901 gaagctttgc caccaccaga gaaaggaaaa taatattgtc gatcccaaga acgatctcca
      961 gcagcgtcag cgtaccgagc gcaagccagg cgttaggatc ggttatccat gcaaataaca
     1021 tctaaaaagc cctgccaaaa aaatgaagcg gtaattatat gcccggaaag cggcaggtca
     1081 aagcgatggc tactggatca gaaaatgacg ggccagtagt gcactggtga aattcttctt
     1141 caaataaaag ccgcgtggca gcgtcagaat ccgttcgccc cgggcaccaa tggcttcggt
     1201 aagcgctttc gcattcgctg ctttcggtct tatctgtaaa tattccccgt gacgagcggt
     1261 gatccgctca acctgaccga gaacaatcat atccattaat tcttcccagt cttcgcgtag
     1321 ctgtcggtct tcctcttcat tcgggctcca cagcaacggt gatcctacgc gacgctgcgc
     1381 cagcgggatg ctgcgctcgc cttcaaccgg tatccacagt acgcgtttga gcttgtggcg
     1441 cacatggctg gtttcccagg tcaccccgct attacccgtt aacggggcaa cacaaacgaa
     1501 tgttgtttcc agcggacgac caagactatc cacagggata gttttaagtt ccacgcccag
     1561 agcagcaaaa tcttgctcag gtttactccc tgcgctggca cctagccaga tctccagtaa
     1621 cacgccaatc cagcccttat cgcgttttaa attctctggc gtaaccagcc cggcaagtgc
     1681 cgccagttct cccaatgtat aaccagaaag ttgctgtgct tgcgctaaca actgttcttc
     1741 agtttcggga ggagagagca gtgggcgagg ttgggacatg tcatgatgcc ttggttaaaa
     1801 aacaaacagc aattatgtgc cgaatattcg cagtgtaact ttttccacat gtgttgttca
     1861 attctctgat ttatattgct ttttaggctg ccggtaatcg agttgcgcaa cactgcaata
     1921 ggttttccaa atctggtcac tgaaaataaa caggatctta caccatgtta tccacagaaa
     1981 gctgggataa ctgtgaaaaa cctccactac tgttttcatt tacagccttg acgtgcgacg
     2041 aaactccagt ttttagtggt tcagtgctta acttagtggc acattctgtg gataaatacg
     2101 gcattggttg atctttcgcc aacccgtaaa tgagaggtgt gacctgggtc acgaattact
     2161 ctttttcatt actcaaatta catgtaatgc attgactaat aatgaatatt ttaaaatgtc
     2221 tatatcgggc ttattcagaa tgctccgggt tttcccgtag taattccgta gttcttcaca
     2281 actctatcca cagaaaaggt gaataaaaac ggctatccac cccttcctct gtttataact
     2341 ctgcacataa ctgtgagtta ttcaattgtt attagtgtgt aatgccctgc agagagtggt
     2401 ttaccgtctt cctggagtat gaaacaatca ttcgtatata aagctttatt ttgaggtagt
     2461 ccggtgattg atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag
     2521 gggcaggtaa tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga
     2581 atcaaccccg gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga
     2641 ttaagccgca aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta
     2701 ccgaaacgtt tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg
     2761 tttctcttgc agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca
     2821 gagtttgacg gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt
     2881 aaacgtgatg tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag
     2941 gaaaatacgc caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg
     3001 ccaattatgc tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg
     3061 aatgaggcgt taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt
     3121 tcggtctacc tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg
     3181 aaaaaaccac gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt
     3241 ggcaggctgg cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac
     3301 atcccctccg taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt
     3361 cgccagttgc ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt
     3421 gaagaatcct tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag
     3481 ttgactgccc tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct
     3541 ggtgtggcga ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat
     3601 caggcatcaa cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa
     3661 gcggcaaacg agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg
     3721 gcggctattt tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg
     3781 tttgccgagg ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa
     3841 tttgccgaac agtttgccgc gctaagcgat aactatctca aagagcgggc tggcgattta
     3901 cgtgcgctgg gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc
     3961 tggccggaac gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg
     4021 ccccaggatc gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg
     4081 atcatggtac gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg
     4141 ctgcatcgta ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag
     4201 ccggtactgc tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg
     4261 gcggaagatg acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg
     4321 ctcaatgctg gtttaagccc ggaacatgaa gaaaaactgg gcagccgtat tgatggcatc
     4381 ggtctttatc gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa
     4441 caggtggcgc agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt
     4501 acgctggatg tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca
     4561 tgcctgggtt ggcgtggtat tcgcattacg ctcgatcagc cggagatctt cttgatccag
     4621 gtgcgggcga tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg
     4681 gtcaccagcc tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa
     4741 gtcgaggaga tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg
     4801 ccgtcaatgg tatttatgct gccgcatctg gcaaagcggg tcgatttcat ctctgttggc
     4861 accaacgatc tgactcaata cattctggcc gttgatcgca acaatacccg ggtggctaac
     4921 atttacgaca gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg
     4981 gaaatacatg gtatcgatct ccgtttgtgc ggtgaaatgg cgggcgatcc catgtgtgtg
     5041 gcaatcctca ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg
     5101 gtaaaatacc tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt
     5161 ctggaagcgc aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc
     5221 ggcatgggcg ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg
     5281 gtatccggca accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa
     5341 cctgcggcgc gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga
     5401 cagatgacca gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga
     5461 cccgtggcgc ttcactggta cggcctgatg tatctggtgg gtttcatttt tgcaatgtgg
     5521 ctggcaacac gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac
     5581 ttactctatg cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc
     5641 tacaatttcc cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc
     5701 atgtctttcc acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt
     5761 actaaacgtt ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt
     5821 ggtgccgggc gtctgggcaa ctttattaac agtgaattgt ggggccgcgt tgacccgaac
     5881 ttcccgtttg ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc
     5941 aacccgcagt ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag
     6001 ctttacgaac tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt
     6061 aaaccgcgcc caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc
     6121 atcattgttg agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac
     6181 atcagcatgg ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc
     6241 tgggcatatc gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta
     6301 gaactgatgc aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga
     6361 acgctttcca tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg
     6421 acaaccaaac gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc
     6481 gatactaaca ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat
     6541 gaaaatggcg acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat
     6601 ggtcgtcata ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg
     6661 cgccgcatta ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg
     6721 tgccatgcat tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag
     6781 cgctcctgtg acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg
     6841 catatgatgg cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat
     6901 acgcacctgt acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt
     6961 ccgctgccga agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa
     7021 gactttgaga ttgaaggcta cgatccacat ccgggcatta aagcgccggt ggctatctaa
     7081 ttacgaaaca tactgccaga gccgacgcca gtgtgcgtcg gtttttttac tctccgttaa
     7141 attcttcgag acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga
     7201 agatgcgccg aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc
     7261 ggcatgaaaa cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg
     7321 gtcatgctaa gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt
     7381 tggcaaaccg ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg
     7441 cataaccgcg accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt
     7501 tccgctgctg gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg
     7561 cccgaagtcg aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc
     7621 gcatgggccg ggcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg
     7681 ccgtggggaa gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc
     7741 aaggtttttc tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg
     7801 gggctgcacg ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg
     7861 cactggaaga tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg
     7921 gttattgtca tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta
     7981 tcattgtgca gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt
     8041 ccgaccagat tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat
     8101 cctgtgaagg taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt
     8161 ttcaggtcgt acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg
     8221 ctgccagtaa gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca
     8281 acctgtgaac cgcgaaaaag gtgtttcgtc acaggctctg gtcctgatgc tgctggtttt
     8341 gggtagcttg ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag
     8401 catggaaagt cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa
     8461 aatgcactcc tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc
     8521 ccaggtttgt ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg
     8581 cgtttcgttg tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg
     8641 ctggagcgat ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa
     8701 caaggcttta gcctgccgga ggtaatggtt gcgatggtgc tgatggtgat gattgtcact
     8761 gcgttatcgg gtatccagcg aacattaatg aacagtctcg ccagcagaaa ccagtaccaa
     8821 cagctctggc ggcatggttg gcagcaaacg caactgcgcg cgatttcgcc acctgccaac
     8881 tggcaggtca accgaatgca gacatcgcag gcgggatgtg tcagcatcag cgttacgcta
     8941 gtttcacccg ggggcagaga aggcgagatg acccgcctgc attgcccgaa tcgtcagtag
     9001 tcaggagccg ctatgttaag ggtctaccat tccaatcgtc tggacgtgct ggaagcgttg
     9061 atggagttta ttgtcgaacg cgaacggctg gacgatcctt tcgaaccaga gatgattctg
     9121 gtgcaaagta ccggtatggc gcagtggctg caaatgaccc tgtcgcaaaa gtttggtatt
     9181 gcggcaaaca ttgattttcc gctgccagcg agctttatct gggatatgtt cgtccgggtg
     9241 ttaccggaaa tccccaaaga gagcgccttt aacaaacaga gcatgagctg gaaactgatg
     9301 actctgctgc cgcaattgct ggagcgcgaa gactttaccc tgttgcggca ttatctgact
     9361 gacgatagcg acaagcgaaa actgttccag ctttcctcaa aagcggcgga cctgtttgac
     9421 cagtatctgg tctatcgtcc ggactggctg gcacagtggg aaacaggaca tttggttgaa
     9481 gggctgggag aagcacaggc ctggcaagcc ccgttgtgga aggcgctggt ggaatatacc
     9541 catgaactcg gacaaccgcg ctggcaccgc gcgaatctct atcagcgctt tatcgaaacg
     9601 ctggagtccg cgacgacctg cccgccgggg ttaccttcgc gcgtctttat atgcggtatt
     9661 tccgcgttac cgcctgttta tctccaggcg ctccaggcgc tgggtaaaca tattgaaatc
     9721 catctcctgt ttaccaaccc ctgccgttat tactggggcg atattaaaga tcctgcttat
     9781 ctggcgaaac tgctgactcg tcagcgtcga cacagttttg aagatcgcga attgccgcta
     9841 tttcgcgata gcgaaaatgc cggacagctc tttaacagcg atggtgaaca ggatgtcggc
     9901 aacccgctgc tggcctcatg ggggaagctt gggcgcgact acatttatct cctttctgac
     9961 ctggagagca gccaggagct ggacgctttt gtcgatgtga cgccagataa cctgctgcat
    10021 aacattcagt ctgacattct ggaactggaa aaccgcgccg ttgcgggtgt gaacatcgaa
    10081 gagttttccc gtagcgataa caaacgcccg cttgatccac tggatagcag tatcacattc
    10141 cacgtttgcc atagcccgca gcgtgaagtt gaagttttac acgatcgctt gctggcgatg
    10201 ctggaggaag ccccgacact tactccgcgc gacatcatcg tgatggtggc tgatatcgac
    10261 agctacagtc cgtttattca ggcagtgttt ggtagcgcac ctgcggatcg ttacctgcct
    10321 tacgccattt ccgaccgtcg tgcgcggcag tcacatccgg tactggaagc gtttatcagc
    10381 ctgttatcac tgcctgacag tcgctttgtg tcagaggatg tgctggcgct gctggatgtg
    10441 ccggtgctgg cggcgcggtt tgacatcacc gaagaagggc tgcgttattt acgccagtgg
    10501 gtcaacgaat ccggcattcg ttgggggata gatgacgaca acgttcgcga gctggaactc
    10561 cccgccaccg gacaacacac ctggcgattt ggcctgacgc gtatgttgct gggctacgcg
    10621 atggagagcg cgcagggcga gtggcaatcg gttctacctt atgatgaatc gagcggctta
    10681 attgcagaac tggtggggca tctggcttca ctgctaatgc agctaaacat ctggcgtcgc
    10741 gggctggcac aggagcgtcc gctagaagag tggttgccgg tttgtcgcga tatgctcaac
    10801 gccttcttcc tgccggatgc ggaaaccgaa gcggcgatga cgctgatcga acaacaatgg
    10861 caggcgatta tcgccgaagg tttaggtgcg cagtatggcg acgcggtgcc gctgtcacta
    10921 ttgcgtgatg aactggcaca gcgtctggat caagaacgta tcagccagcg ttttctcgcc
    10981 ggaccggtta atatttgtac tctgatgcca atgcgttcaa ttccgttcaa agtggtttgc
    11041 ctgctgggaa tgaacgacgg cgtttatcca cgtcagcttg cgccattggg ctttgacctg
    11101 atgagccaga aaccgaagcg tggcgaccgt agccgtcgcg atgacgaccg ctacctgttc
    11161 ctggaagcgt taatttccgc gcagcaaaaa ctctatatca gctatatcgg gcgttccatt
    11221 caggataaca gtgaacgttt cccgtcggta ctggtgcagg aactgatcga ctacatcggg
    11281 caaagtcatt atctaccggg cgatgaagcg ctcaactgtg atgaaagcga ggcaagggta
    11341 aaagcgcatc ttacttgcct ccatacccgg atgccgtttg atccgcaaaa ctaccagcca
    11401 ggcgaacgac aaagctatgc tcgtgaatgg ctacctgcgg ccagccaggc tggtaaagca
    11461 cattctgaat ttgttcagcc gctgccgttt accttaccgg aaaccgtgcc gctggaaacg
    11521 ctacaacgat tctgggcaca tccggtgcgg gcgttcttcc agatgcgttt gcaggtgaac
    11581 ttccgtactg aagacagcga aatcccagac accgagccat ttattctgga aggacttagc
    11641 cgttatcaaa tcaatcagca gttattgaat gcactggttg agcaggatga tgccgaacgc
    11701 ttgttccgcc gcttccgggc ggcaggggat ttaccgtatg gcgcttttgg tgaaattttc
    11761 tgggaaacac agtgccagga gatgcagcag cttgccgaca gagtcattgc ctgtcgccag
    11821 ccggggcaga gtatggaaat tgatctcgcc tgcaacggtg tgcagataac tggctggttg
    11881 ccgcaggtgc agccggatgg cctgttgcgc tggcgtccct ctttattaag tgtggcgcag
    11941 ggaatgcaac tttggctgga acaccttgtc tactgtgcca gcggtggtaa tggcgaaagt
    12001 cgcctttttc tacgcaaaga cggtgagtgg cgttttccgc cgcttgcagc cgaacaggct
    12061 ttgcattacc tctcacaact gattgagggg tatcgtgaag gaatgtccgc gccattgctg
    12121 gtgttacctg aaagtggcgg cgcgtggcta aaaacctgtt atgacgcgca aaacgatgcc
    12181 atgctggatg acgattccac gttgcaaaaa gcccgtacga aattccttca ggcttacgaa
    12241 ggcaacatga tggtgcgtgg cgaaggtgat gatatctggt atcaacggct ctggcggcaa
    12301 ttaacaccag agacaatgga ggccatcgtt gaacagtcgc aacgtttcct gttaccgctg
    12361 tttcgcttta atcagtcatg agtgttgtat aaaaattgcg caatctatcc gcttacttta
    12421 tgatgcgcac cagtcacgga ctgatggtta tataaacata ggctgactcg tgcagcacaa
    12481 gattaaattc tggcagatga tttgcgttaa cctgttgaat ctgtccagaa aataaagttg
    12541 attatgaggt ccgtgaatgc cccgcagcac ctggttcaaa gcattattgt tgtttgttgc
    12601 cctctgggca ccattaagtc aggcagaaac gggatggcag ccgattcagg aaaccatccg
    12661 taaaagtgat aaagataacc gccagtatca ggctatacgt ctggataacg gtatggtggt
    12721 cttgctggtt tctgatccgc aggcagttaa atcgctctcg gcgctggtag tgcccgttgg
    12781 gtcgctggaa gatcccgagg cgtaccaggg gctggcacat taccttgaac atatgagtct
    12841 gatggggtcg aaaaagtacc cgcaggctga cagtctggcc gaatatctca aaatgcacgg
    12901 cggtagtcac aatgccagca ctgcgccgta tcgcacggct ttctatctgg aagttgagaa
    12961 cgacgccttg cccggtgcgg tagaccgcat ggccgatgct attgcagaac ccttgctcga
    13021 caagaaatac gccgaacgtg agcgtaatgc agtgaacgct gaattaacca tggcgcgtac
    13081 gcgtgacggg atgcgcatgg cacaggtcag cgcagaaacc attaacccgg cacaccccgg
    13141 ttcaaagttt tctggtggta acctcgaaac tttaagcgac aaacctggta atccggtgca
    13201 gcaggcgctg aaagatttcc acgagaagta ctattccgcc aatttgatga aggcggttat
    13261 ttacagtaat aaaccgctgc cggagttggc aaaaatggcg gcggacacct ttggtcgcgt
    13321 gccgaacaaa gagagcaaaa aaacggaaat caccgtgccg gtagtcaccg acgcgcaaaa
    13381 gggcattatc attcattacg tccctgcgct gccgcgtaaa gtgttgcgcg ttgagtttcg
    13441 catcgataac aactcagcga agttccgtag taaaaccgat gaattgatta cctatctgat
    13501 tggcaatcgc agcccaggta cactttctga ctggctgcaa aagcagggat tagttgaggg
    13561 cattagcgcc aactccgatc ctatcgtcaa cggcaacagc ggcgtattag cgatctctgc
    13621 gtctttaact gataaaggcc tggctaatcg cgatcaggtt gtggcggcaa tttttagcta
    13681 tctcaatctg ttacgtgaaa aaggcattga taaacaatac ttcgatgaac tggcgaatgt
    13741 gctggatatc gacttccgtt atccgtcgat cacccgtgat atggattacg tcgaatggct
    13801 ggcagatacc atgattcgcg ttcctgttga gcatacgctg gatgcagtca atattgccga
    13861 tcggtacgat gctaaagcag taaaggaacg tctggcgatg atgacgccgc agaatgcgcg
    13921 tatctggtat atcagcccga aagagccgca caacaaaacg gcttactttg tcgatgcgcc
    13981 gtatcaggtc gataaaatca gcgcacaaac tttcgccgac tggcagaaaa aagccgccga
    14041 cattgcgctc tctttgccag agcttaaccc ttatattcct gatgatttct cgttgattaa
    14101 gtcagagaag aaatacgacc atccagagct gattgttgat gagtcgaatc tgcgcgtggt
    14161 gtatgcgcca agccgttatt ttgccagcga gcccaaagct gatgtcagcc tgattttgcg
    14221 taatccgaaa gccatggaca gcgcccgcaa tcaggtgatg tttgcgctca atgattatct
    14281 cgcagggctg gcgcttgatc agttaagcaa ccaggcgtcg gttggtggca taagtttttc
    14341 caccaacgct aacaacggcc ttatggttaa tgctaatggt tacacccagc gtctgccgca
    14401 gctgttccag gcattgctcg aggggtactt tagctatacc gctacggaag agcagcttga
    14461 gcaggcgaag tcctggtata accagatgat ggattccgca gaaaagggta aagcgtttga
    14521 gcaggcgatt atgcccgcgc agatgctctc gcaagtgccg tacttctcgc gagatgaacg
    14581 gcgtaaaatt ttgccctcca ttacgttgaa agaggtgctg gcctatcgcg acgccttaaa
    14641 atcaggggct cgaccagagt ttatggttat cggcaacatg accgaggccc aggcaacaac
    14701 gctggcacgc gatgtgcaaa aacagttggg cgctgatggt tcagagtggt gtcgaaacaa
    14761 agatgtagtg gtcgataaaa aacaatccgt catctttgaa aaagccggta acagcaccga
    14821 ctccgcactg gcagcggtat ttgtaccgac tggctacgat gaatacacca gctcagccta
    14881 tagctctctg ttggggcaga tcgtacagcc gtggttctac aatcagttgc gtaccgaaga
    14941 acaattgggc tatgccgtgt ttgcgtttcc aatgagcgtg gggcgtcagt ggggcatggg
    15001 cttccttttg caaagcaatg ataaacagcc ttcattcttg tgggagcgtt acaaggcgtt
    15061 tttcccaacc gcagaggcaa aattgcgagc gatgaagcca gatgagtttg cgcaaatcca
    15121 gcaggcggta attacccaga tgctgcaggc accgcaaacg ctcggcgaag aagcatcgaa
    15181 gttaagtaaa gatttcgatc gcggcaatat gcgcttcgat tcgcgtgata aaatcgtggc
    15241 ccagataaaa ctgctgacgc cgcaaaaact tgctgatttc ttccatcagg cggtggtcga
    15301 gccgcaaggc atggctattc tgtcgcagat ttccggcagc cagaacggga aagccgaata
    15361 tgtgcatcct gaaggctgga aagtgtggga gaacgtcagc gcgttgcagc aaacaatgcc
    15421 cctgatgagt gaaaagaatg agtgatgtcg ccgagacact agatcctttg cgcttgccct
    15481 tacagggcga gcgcctgatt gaagcctctg ccggcacagg caaaaccttt acgattgcgg
    15541 cgctctattt gcgcctgtta cttggactag gcggttccgc cgcctttccc cgcccgctga
    15601 tcgttgaaga actgctggtg gtcaccttta ccgaggctgc cacggcagaa ttgcgcggtc
    15661 gtatccgtag caatatccac gagttgcgca tcgcctgtct gcgtgaaacc accgacaatc
    15721 cactgtacga acgcctgctg gaagagatcg acgataaagc gcaagccgcg cagtggttgt
    15781 tgttagccga acggcagatg gatgaagcgg cagtctttac tattcacggc ttttgccagc
    15841 gcatgctcaa cctgaatgcc tttgaatccg gcatgctgtt tgagcagcag ctgattgaag
    15901 atgagtctct gctacgctac caggcctgcg ccgatttctg gcgtcgccac tgctacccgc
    15961 tgccgcgtga aatagcccag gtcgtctttg aaacctggaa agggccgcag gcgttgctgc
    16021 gcgatattaa tcgttatctg caaggcgaag cgccggttat caaagcaccg ccgcccgatg
    16081 atgaaacgct ggcttcccgc cacgcgcaaa ttgtggcgcg tattgatacg gtaaaacagc
    16141 agtggcgcga cgcagtgggt gaactggatg cgctgatcga atcttctggt attgatcgac
    16201 gcaagtttaa ccgtagcaat caggctaaat ggatcgaaaa gatcagcgct tgggcagaag
    16261 aagagacaaa cagttatcag ttgccggagt cgctggaaaa attctcccag cgtttcttag
    16321 aagatcgcac gaaggccggg ggggaaaccc cgcgacatcc actgtttgag gtgatcgatc
    16381 aactgcttgc agaaccattg tcgatccgcg atctggtgat cacccgagca ttggctgaga
    16441 tccgcgaaac agtagcgcgt gaaaaacgcc gccgtggcga attgggtttt gatgacatgt
    16501 taagtcggct cgattccgcg ctgcgtagcg aaagcggcga ggtgttggca gcggcgatcc
    16561 gtacgcgatt cccggtggca atgatcgatg aatttcagga taccgacccc cagcagtacc
    16621 gaatttttcg ccgtatctgg caccatcagc cggaaaccgc attgttgctg attggcgacc
    16681 cgaagcaggc catatatgca ttccggggtg cggatatctt cacttatatg aaggcgcgta
    16741 gtgaagttca cgcccactac actttagaca ccaactggcg ctccgcacct ggaatggtga
    16801 acagtgtgaa caagcttttc agccagactg atgacgcgtt catgtttcgc gaaataccgt
    16861 ttattccagt gaaatcagcc gggaaaaatc aggcgttacg ttttgtattt aaaggtgaaa
    16921 cgcagcctgc gatgaaaatg tggctgatgg aaggcgaaag ctgcggcgtt ggcgactatc
    16981 aaagtaccat ggcgcaggta tgtgctgcgc aaatccgcga ctggctacaa gccggacagc
    17041 ggggcgaagc gttgctgatg aacgacgacg acgcgcgtcc ggtgcgtgct tcggacatca
    17101 gcgtgctggt gcgcagccgc caggaggccg cccaggtgcg cgatgcctta acgttgctgg
    17161 aaatcccttc cgtttacctt tcgaaccgcg acagtgtttt tgaaactctg gaagcgcagg
    17221 aaatgctttg gttgttgcag gcggtgatga cgcccgaacg tgagaacacc ctgcgcagtg
    17281 cgctggcaac gtcaatgatg gggctgaatg cgctggatat tgaaacgctg aataatgacg
    17341 aacatgcgtg ggatgcggta gtcgaagagt tcgatggtta tcggcaaatc tggcgcaaac
    17401 gtggcgttat gccgatgctg cgggcgctga tgtcggcgcg taacattgca gaaaacttgc
    17461 tggcaacggc aggcggtgag cggcgtctta ccgatatctt gcatatcagc gaactgcttc
    17521 aagaagccgg aacgcagctg gaaagtgaac atgcgctggt acgctggtta tcgcaacata
    17581 tcctcgagcc agacagtaat gcctccagcc aacaaatgcg cctcgaaagt gataaacatc
    17641 tggtgcagat tgtcacgatc cataaatcga aagggctgga atatccgctg gtctggttgc
    17701 catttattac caatttccgc gtccaggatc aggcgtttta tcacgatcgc cactcgtttg
    17761 aggcagtttt ggatcttaat gctgcgccag aaagcgtcga cctcgcggag gtcgaacgtc
    17821 tggcggaaga tctgcgtttg ctttacgtgg cgctgacgcg ttcggtttgg cattgcagtc
    17881 tcggcgttgc accgctggtg cgccgtcgtg gcgataaaaa aggtgacacc gacgtccacc
    17941 aaagtgcgct cgggcgtttg ctgcaaaaag gggaaccgca agatgcggca gggcttcgca
    18001 cctgtattga agcgttatgc gatgatgata ttgcctggca aacggcacaa actggtgata
    18061 accagccctg gcaggttaat gatgctttaa ctgcagaact gaatgcgagg acactacaac
    18121 gattgcccgg cgataactgg cgtgtcacca gctactccgg cttgcagcag cgtggtcacg
    18181 gtatcgctca ggatctgatg ccacggctgg atgttgatgc cgcaggcgtg gtcagcgtcg
    18241 ttgaagaacc gacgttaaca ccgcatcagt tcccgcgcgg tgcgtcaccg gggacattct
    18301 tgcacagttt gtttgaagac ctcgatttta cccagccggt tgacccgaac tgggtacagg
    18361 aaaaactgga actcggcggc tttgaatcgc agtgggaacc ggtgttgacc gagtggatca
    18421 cggctgtcct ccaggcacct ctcaatgaaa cgggtgttag cctgagtcag ctttccgacc
    18481 gcgataaaca ggtggagatg gagttttacc tgccgattag cgaaccgctc atcgccagcc
    18541 agcttgatgc actaattcgc cagtttgacc cgctatccgc tggctgcccg ccgctggagt
    18601 tcatgcaggt acgcggcatg ttaaaaggct ttatcgacct ggtattccgc cacgaagggc
    18661 gttattacct gctcgactat aaatccaact ggctgggtga agacagttcg gcttacaccc
    18721 aacaggctat ggcagcagcg atgcaggcac accgctatga tctgcaatat cagctttata
    18781 ccctggcact gcatcgttat ctgcgccatc gcattgctga ttacgactat gatcttcact
    18841 ttggcggcgt tatctatctg ttcctgcgtg gcgttgataa agaacatccg caacaaggga
    18901 tttacacaac ccgacccaac gccgggttga ttgccctgat ggatgagatg tttgccggta
    18961 tgaccctgga ggaggcgtaa tgaaattgca aaagcaatta ctggaagctg tggagcacaa
    19021 acagctacgc ccgctggatg tgcaatttgc cctgaccgtg gcgggagatg aacatcctgc
    19081 tgtcaccctc gcggcggcac tgttaagtca tgatgccgga gagggacacg tctgtttgcc
    19141 gctttcacga ctggaaaata acgaggcgtc gcatccgctg ttggcgacct gtgtcagtga
    19201 aatcggtgag ctacaaaatt gggaagaatg cttgctggct tctcaagcgg tcagcagggg
    19261 agatgaaccc acgccgatga tcctctgtgg cgatcgtctt tatttgaatc gcatgtggtg
    19321 taacgagcgg acggtagcgc gcttcttcaa tgaggtgaat cataccattg aggttgatga
    19381 agctctactg gcgcaaaccc tggacaaact ttttccagta agcgatgaaa ttaactggca
    19441 aaaagttgca gcggcagtgg cgctgacgcg gcggatctcg gtgatttccg gcggcccagg
    19501 caccggtaaa acgaccaccg tggcgaagtt gctggcagcg ttaattcaaa tggccgacgg
    19561 cgaacgctgc cgtatccgtc tggctgcacc aacgggtaaa gctgccgcgc gcttaaccga
    19621 atctctcggc aaggctttgc gacagttacc gctgaccgat gaacaaaaga aacgcattcc
    19681 ggaagatgcc agcactttgc accgattgct gggcgcgcag ccgggtagcc agcgtttacg
    19741 tcatcatgcc ggtaacccgc tgcatcttga tgtgctggtg gtagatgaag cgtcaatgat
    19801 cgatctgcct atgatgtcga gactgatcga cgccttgcca gatcatgcgc gagtgatctt
    19861 tctcggcgat cgtgatcaac tggcctcggt tgaggctggg gctgtgctgg gcgatatctg
    19921 cgcttatgcc aacgcgggct ttaccgccga gcgtgccagg cagctaagcc gcctgacggg
    19981 gactcacgtt ccggcaggaa ccggcacaga agcggcatct ttgcgcgaca gtctctgcct
    20041 gctgcaaaaa agctatcgtt tcggcagcga ttctggcatt ggtcagttag ctgcggcgat
    20101 caaccgtggt gataaaacgg cagtgaaaac cgtttttcag caggatttta ctgatatcga
    20161 aaaacgactt ttacagagcg gcgaagatta tattgcgatg cttgaggaag ctcttgcggg
    20221 ttacggacgt tatctggatc tgctgcaagc gcgtgccgag ccggatttaa tcattcaggc
    20281 gttcaatgag taccagcttt tgtgcgccct gcgggaaggg ccgtttggcg tggctggact
    20341 gaatgagcga attgagcagt ttatgcaaca gaagcgcaaa attcatcgtc atccgcactc
    20401 tcgttggtac gaaggccgac cggtgatgat tgcccgtaat gacagcgcgc ttgggttgtt
    20461 taatggcgat atcggtattg cgctggatcg cgggcagggg acgcgcgtct ggtttgcgat
    20521 gccggacggc aatattaagt ctgtgcaacc gagtcgcttg ccagagcacg aaactacgtg
    20581 ggcgatgacg gtacataaat cgcagggatc ggagttcgac catgcggcgt tgattttacc
    20641 gagccaacgc acgccggtag taacgcgaga gctggtatac accgcggtga cccgcgcgcg
    20701 tcgtcgtctg tcgctgtatg ccgatgagcg tatattaagt gcggcaatcg ccactcgtac
    20761 tgagcggcgc agtggtctgg cggcgttgtt tagttcgcgg gaataaacgt aattgccgga
    20821 tgcgacgcac gagtgttacg cctgccgcat ccggtgattt tcatcgctta ccctaaatcc
    20881 gccatcaaca ctttggattt acgctggtag ttgtacaact gctttttgct ctcgggcagt
    20941 aaatcaatat ccaccggggt aaatccacgt tcctggaacc agtgaatact gcgcgtggtc
    21001 agcacaaaca atttgcttaa gccgctctgc ttcgcctgag cggcaatgcg ttccagcaga
    21061 acctcgcccc gtgatgaact gcggtaatcc gggtgaactg ccacacaggc catttcccca
    21121 atcttctctt ccgggaacgg atagagcgcg gcgcaggcaa tagtcgtgtt atcgcgctga
    21181 ataatggtga atttgtcgat ttccatctcc agctgctcgc gagaacggcg caccagaata
    21241 ccttgctgct ccagcgggcg aatcaactcc agaataccgc caatatcgtt gattgttgcg
    21301 cgacgaatct gctcggcgct ttccatcaca atctgcgtac cgataccgtc gcgtgagaac
    21361 aactcttgca acagcgcgcc atcttcctga taactgatta aatgacagcg gcgcacgccg
    21421 ctgcggcagg ctttcactgc gccacgcaaa aagcgcaccg taccggagtt gtaatcgcct
    21481 ttctcttcct gggcttctac ccgtgcttgc gcttcgttag ggaaaagttc ggagacaata
    21541 tcaccgtcgt cattagtaac gccctgggaa gagcaaaaac caatcatctt ttcagctttc
    21601 agtttgatgg ctagttgagt ggcaatctct tcagaggtca gattaaagct ctcgccagtg
    21661 actgaaacag cgaccggccc cattagcact attgcaccgc tttccagttg acgatggatc
    21721 gcgtcttcat caatccgccg gatacgcccg ctatggcagt aatccacgcc gtcatcgacg
    21781 cccagcggct gggcaataat aaaattgcca ctaacgacgt tgatatgcgc gccctgcagc
    21841 ggcgtgttat tgagactcat tgacaggcga gcagtaatat ccagttgcaa tgttcccgca
    21901 gcctgcttca ccagttccag tgttttggcg tcggtcacac gtatattctt gtgatacagc
    21961 ggttcgtggt gatgcgcagc cagatttgcg tcgatctgcg gacgtgcgcc atagaccacc
    22021 accagacgga tgccgaggct gtgcaacaac ccgatatcat taacgatact ggagaaattc
    22081 tcatgctcaa tggcttcacc gccgagcatg atgacaaacg tttttccccg gtgggtattg
    22141 atatagggaa ccgaatggcg gaatccctcg accagctcgg ttttacgttc ctttaccacg
    22201 gcacacccct ttgcatgatt atttgaaatt attgtatttt tattctgttt tttcgcaggg
    22261 tgcaagtgta aattttatgc gagagcgact tttttatcag taatgccgtg aatataaaaa
    22321 gaatgtttac cgtttattag atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa
    22381 ttagtcgttt acttgttaca cagcttagat ttcttttgct cgggagaggc atgtcaggat
    22441 ccaacactgc aatcagccgt cgtcgtttac tgcaaggcgc gggcgctatg tggctattga
    22501 gcgtaagtca ggtcagcctg gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg
    22561 cgtccagcta cacccgcgtg acggtagaat caaatcgtca gctgaaatat aagcagttcg
    22621 cgttgagtaa ccctgaacgc gtggtggtgg atatcgaaga tgtaaacctg aactcggtac
    22681 tcaaggggat ggcggcacaa attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg
    22741 ggcaatttga cccgcaaacc gtacgcatgg tttttgaatt aaagcaaaac gtaaaaccgc
    22801 agctgtttgc ccttgcgccg gtcgccgggt ttaaagagcg tctggtgatg gacctctatc
    22861 cggccaatgc acaggatatg caggacccgc tgctggcgct gctggaggat tacaacaaag
    22921 gcgacctcga aaagcaggtg ccgccagcgc aaagtggtcc acaaccgggt aaagcagggc
    22981 gcgatcgtcc gattgtcatt atgcttgacc ctggccacgg tggcgaagat tccggtgcgg
    23041 tggggaaata caaaacgcgc gaaaaagatg tggtattgca aatagctcgc cgtttgcgct
    23101 ctctgatcga gaaagagggc aatatgaagg tgtacatgac gcgcaatgaa gacatcttca
    23161 ttccgttgca agtgcgcgta gcaaaagccc agaaacagcg tgctgacctg tttgtctcta
    23221 tccatgccga cgcctttacc agtcgtcagc cgagcggttc ctcggtgttt gcgctctcaa
    23281 ccaaaggcgc aaccagtact gcggcaaaat atctggcaca aacccagaac gcctcggact
    23341 tgattggtgg cgtgagcaaa agcggtgacc gctatgtcga ccacaccatg ttcgatatgg
    23401 tgcagtcgct gactattgct gacagcctta agtttggtaa agcggtactg aataagctcg
    23461 gaaaaatcaa caagctgcat aaaaatcaag ttgaacaggc cgggtttgcc gtactaaagg
    23521 caccagatat tccctccatt ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc
    23581 gtaaactgaa aacggcgact ttccagcagg aagttgcgga gtctattctt gcggggatta
    23641 aagcgtattt tgccgatggg gcaacgctgg cgagaagggg atgataaaaa ggcgctgaat
    23701 ggcgcttttt ttatgggaga tcagaaacaa aaaaacaccc gttagggtgt tcaataattg
    23761 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    23821 ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc atgcaacctt
    23881 ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca ctaaagctta
    23941 ttttttgtgc gaaatttgcg tcagggagag gctgctcatt tcattaaacg cggcggtttg
    24001 ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt
    24061 aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac
    24121 cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca
    24181 gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa ttaaccatat
    24241 ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga
    24301 gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca
    24361 gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat
    24421 tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta tgccgccaaa
    24481 acgtggtcgt ctgccgtctc gtgcggagat ctacgccggg gcattgggcg ataaatacat
    24541 cctcgcttac agcaactctc tgatggataa cttcattatg gatgtgcagg gtagtgggta
    24601 tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca
    24661 tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat
    24721 gtcgatgcag gcgattcgtc attggggcaa aacacacagt gaagccgagg ttcgcgagct
    24781 gctggaacag aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg
    24841 ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc
    24901 gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg
    24961 tcagtacgaa ttgcgtctga tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca
    25021 cttcgatatc tatcaaggga tcgggccgga agccggacac cgcgcaggtt ggtacaacca
    25081 ctatggacgg gtttgggtgc tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg
    25141 atgtgatatt ctgtatgccg tttttacgga tatgacaggg tgaggggtaa cctcacatcc
    25201 tgattattga ctaagtgcgc gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc
    25261 tacaaatcat gcaaatccaa aatattgcag gagttatgta ggcctgataa gcgtagcgca
    25321 tcaggcattt atgcgtttag ggtgagggat aacctcgccc tttttaattc tgaggtgcta
    25381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
    25441 gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
    25501 tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg
    25561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
    25621 tcgggctggc aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc
    25681 gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg
    25741 gttattcgta cgtgattgat gccattgata gtgtacggcc caaagcggcg ctgattgctt
    25801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
    25861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggatccgctg gcggcgaagt
    25921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
    25981 tggattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg
    26041 cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg
    26101 caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga
    26161 tggcgaaagc ggcgcgtcag ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc
    26221 cgacccggtt ctgtgcttaa acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat
    26281 ttaacccctg gctgcgtgag gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg
    26341 ccagcggcga ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca
    26401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaagtgc attttgccgt
    26461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
    26521 gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat
    26581 atttatcttc ccattgcgac aacggtgcga aggtattgcg taacgtttct gcggttacgg
    26641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
    26701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
    26761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgttg
    26821 cccggcccgc aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac
    26881 gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
    26941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg
    27001 gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga cgccagcgac
    27061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtggtgaaac cgtcaaaact
    27121 cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga
    27181 tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata
    27241 gaaatcgata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
    27301 aaccatcacc accatcccgg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca
    27361 accgccagtc acgttcgaca tctgacccaa cgccagaatc cgactacggg gagtaatcag
    27421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
    27481 ggctctagtt tgttgggcga ccatcagcca ggggacgagg ttagcgtggt gttctgccac
    27541 gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat
    27601 gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag
    27661 taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc
    27721 aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac
    27781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
    27841 tgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
    27901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
    27961 tatctagata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa
    28021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
    28081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
    28141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
    28201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
    28261 tcaaccgtac tgacgtgaaa gagatggtcg aactggaaca gtaattcgcg atcgcaaggt
    28321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
    28381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
    28441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaatacg cattccgata
    28501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
    28561 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtgatctaat
    28621 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
    28681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt tttactcgcg cagcagaaga
    28741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg
    28801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
    28861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
    28921 ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
    28981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
    29041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
    29101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
    29161 ttcgccgcta ctgctgacag gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca
    29221 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
    29281 aaatcatatc aacgttcagc aagggccaat ttttagtcat agcgccatgg tgctgcaagc
    29341 ggctattcac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
    29401 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaatg ctttttatct
    29461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
    29521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
    29581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
    29641 tgtggcactg ggcgcttttg gtgcgcatgt gttaagtaaa accatggggg ccgttgagat
    29701 gggctggatc caaaccggtc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
    29761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
    29821 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
    29881 gtgggcgttt gtcacaccgg ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt
    29941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
    30001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
    30061 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat gaatgttatc
    30121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
    30181 gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
    30241 ccattgtcgg catgttacag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg
    30301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taagttcact gttccgctac
    30361 gcgctgcctt gcgcgatgct ggggtgctgg cgaactatga aacgccgaag cgtccggttg
    30421 tgcatgtatt ctttattgca ccaggatgct gctataccgg ttactcatac agcaacaata
    30481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt
    30541 ccacgctcaa actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac
    30601 gcctggcgaa cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc
    30661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
    30721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
    30781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat
    30841 tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc
    30901 cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg
    30961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
    31021 tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
    31081 aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
    31141 agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat acccgatgcg
    31201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
    31261 ttttaatagc atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct
    31321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
    31381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
    31441 cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
    31501 ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa
    31561 ggctatcacc atcctttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
    31621 cgcataatgg cttttcagcc tgatgtgaaa gagagcgcta ctgtcttgat tttgacggtg
    31681 gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag
    31741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
    31801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
    31861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
    31921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
    31981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
    32041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
    32101 accctctacc gccgccatca tcaccagcgg cggggcataa ctgtccagct caaataacgg
    32161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
    32221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc
    32281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
    32341 cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
    32401 gtgcacgggc ttcttccggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag
    32461 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
    32521 ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat aattcatagg
    32581 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gcagttaacc
    32641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact ccttgccagc
    32701 cagcgttctg acacgacaat aaatcacact gcattttcac accatccgcg ccaggtgcga
    32761 tcagacgagc ttcttcaatc aacatttgcc agggtgtttc agccgtccag aacagttttc
    32821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccatgcct
    32881 ggctatctag ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta tcaacctagg
    32941 cgctgcgaac cattaaaatt tcccatgtac cggaggagag cacgggttca ttttgttcgg
    33001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
    33061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
    33121 ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt tgcggactga
    33181 aatcgcgttg ctggatatcc agcatctggc tggttccggc tatcgtgata tcagtagtga
    33241 attcgccggt taaacggtgg ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt
    33301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
    33361 aggctccgac gccagaaata gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt
    33421 ccataaccgc tgctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
    33481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc
    33541 attcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcag
    33601 tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt
    33661 gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg
    33721 ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat atagccggat
    33781 aacattgttt atccggctaa ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg
    33841 gcatcacatc agggtgtaat gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg
    33901 cggtaatcct ggccttcaat atccatgccg tgcgcagccc aggcagaagg acgatacact
    33961 ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc aagggtgata
    34021 aagtctgcgc caacgtggcc gatggtcaga actccgtggt tggcacccca gttcgccatt
    34081 accgaataca catccgtaaa cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt
    34141 ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg caattccacg
    34201 ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt gacacgagtc
    34261 atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc gccacggaag
    34321 tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc ctcttgctga
    34381 gagatttccc agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa
    34441 ccgtccagcg cagcagaacc tgagttgatc aaatggatga tgccgtgttc tgccagtccg
    34501 tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt acgcacatcg
    34561 gcaaatacct gagcggtgcc ggtgagctgg tgacccatta gcattgccac gccgttaaga
    34621 ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc cattccagtc aaatgaactg
    34681 ttgaggatcg cttcggcggt gtcaccattg ggatattgat cggtccagtg acgttgcccc
    34741 tggaagcccg cagcgatggc gttgtagcca agtgattctt ccacgcgacc aatatcggcc
    34801 agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa actttcgcgc
    34861 agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc atcttcgcca
    34921 tagcggaagt ttttatcagc ccaggccagt gccatttcca gttcggcttc gtcgtaaatc
    34981 ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt cattcccagc
    35041 caggattcaa agaagttgtg atcaacaata gaaccggcga tacccatcga aacgccgccc
    35101 agcgacagat agcttttacc tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc
    35161 agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga
    35221 ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc cgctgccagg
    35281 taaacagcgc cggggcgttc agtgccgtta aagccccaaa tggctttcgg gcgggttgga
    35341 tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct
    35401 acattctggc tgctgaattt ttcttcgcaa gcagcggctt cagccatacc cgcgatacag
    35461 gtatcggaaa tgacacactc gacggcagct ccgcaggtat ggcgcagttt ctcggtcagc
    35521 agtgcggccg tagctttcgc catattcatt gtttgttctt caagcgactc acgaacaccc
    35581 atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat ttttttcatt
    35641 acagattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga gaacggaaac
    35701 gggcaaagat aaagatgacc gcgaagcaga gtgcggggat cagttcagca gtggggatgt
    35761 tgcccgccgc gtcactgaca aaacccatga ccggagtgac aataccgccg ccaataatgg
    35821 tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta atgcccagcg
    35881 agaagattgt tgggtactga atcgacataa aggcgctgca taaagtcagg gctattaagc
    35941 ccacatgacc gccagcgaag gctgagatca ggcacagtgc catagcgatt aatgcgtagg
    36001 cggccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg atacgaccaa
    36061 taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgca gtcatacctg
    36121 gaatttcttc tacagcgtag cgaatcaaat agctccagca ggccgtttgt gcgccgacat
    36181 agcagaattg cgctaatacc gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg
    36241 atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat gccgggaatt
    36301 tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc atataaggtg
    36361 tctgtaccga taatactagg ctgtgtttat acgcactcaa ttgctctgga gacattttat
    36421 cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc ccaaagacaa
    36481 ccgcgataat tgcgc
//
LOCUS       DAJTRQ010000023        36265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000023 DAJTRQ010000000
VERSION     DAJTRQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="OO332_001298"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="OO332_001298"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5941845.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="OO332_001299"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="OO332_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5941846.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="OO332_001300"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="OO332_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5941847.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="OO332_001301"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="OO332_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5941848.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="OO332_001302"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="OO332_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5941849.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="OO332_001303"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="OO332_001303"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5941850.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO332_001304"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO332_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5941851.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO332_001305"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO332_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5941852.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="OO332_001306"
     CDS             complement(10310..11188)
                     /locus_tag="OO332_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5941853.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO332_001307"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO332_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5941854.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="OO332_001308"
     CDS             13815..14150
                     /locus_tag="OO332_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5941855.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="OO332_001309"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="OO332_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5941856.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="OO332_001310"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="OO332_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5941857.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="OO332_001311"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="OO332_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5941858.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="OO332_001312"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="OO332_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5941859.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="OO332_001313"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="OO332_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5941860.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="OO332_001314"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="OO332_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5941861.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="OO332_001315"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="OO332_001315"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5941862.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="OO332_001316"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="OO332_001316"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5941863.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="OO332_001317"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="OO332_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5941864.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="OO332_001318"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="OO332_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5941865.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="OO332_001319"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="OO332_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5941866.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="OO332_001320"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="OO332_001320"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5941867.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAHLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="OO332_001321"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="OO332_001321"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5941868.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="OO332_001322"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="OO332_001322"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5941869.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="OO332_001323"
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="OO332_001323"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5941870.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(25844..26173)
                     /locus_tag="OO332_001324"
     CDS             complement(25844..26173)
                     /locus_tag="OO332_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941871.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="OO332_001325"
     CDS             complement(26242..26874)
                     /locus_tag="OO332_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5941872.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="OO332_001326"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="OO332_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="OO332_001327"
     CDS             29284..30804
                     /locus_tag="OO332_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5941873.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="OO332_001328"
     CDS             30801..31775
                     /locus_tag="OO332_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5941874.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="OO332_001329"
     CDS             31806..32747
                     /locus_tag="OO332_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5941875.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="OO332_001330"
     CDS             32802..33485
                     /locus_tag="OO332_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCR5941876.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="OO332_001331"
     CDS             33500..34360
                     /locus_tag="OO332_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCR5941877.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="OO332_001332"
     CDS             34357..35358
                     /locus_tag="OO332_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5941878.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="OO332_001333"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="OO332_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         8427 a        10154 c         9644 g         8040 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tataattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tccggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gactacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggtacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc tgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgtcgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctactg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctaggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccacccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgacgcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatgattc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg cgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttccgtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcatcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccaccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcatattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tactggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gcaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagt gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagca gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccgctcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctct ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgcttcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacaga
    33001 ggctgacgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaa
//
LOCUS       DAJTRQ010000024        35276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000024 DAJTRQ010000000
VERSION     DAJTRQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..186)
                     /locus_tag="OO332_001334"
     CDS             complement(<1..186)
                     /locus_tag="OO332_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5941879.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAA"
     gene            complement(187..273)
                     /locus_tag="OO332_001335"
                     /pseudo
     CDS             complement(187..273)
                     /locus_tag="OO332_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(367..1842)
                     /locus_tag="OO332_001336"
     CDS             complement(367..1842)
                     /locus_tag="OO332_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5941880.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(1989..3273)
                     /locus_tag="OO332_001337"
                     /pseudo
     CDS             complement(1989..3273)
                     /locus_tag="OO332_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3386..5320)
                     /gene="yeaG"
                     /locus_tag="OO332_001338"
     CDS             complement(3386..5320)
                     /gene="yeaG"
                     /locus_tag="OO332_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5941881.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5756..6502
                     /gene="mipA"
                     /locus_tag="OO332_001339"
     CDS             5756..6502
                     /gene="mipA"
                     /locus_tag="OO332_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5941882.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6592..7353
                     /locus_tag="OO332_001340"
     CDS             6592..7353
                     /locus_tag="OO332_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5941883.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7404..8288)
                     /locus_tag="OO332_001341"
     CDS             complement(7404..8288)
                     /locus_tag="OO332_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5941884.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8372..9367)
                     /gene="gapA"
                     /locus_tag="OO332_001342"
     CDS             complement(8372..9367)
                     /gene="gapA"
                     /locus_tag="OO332_001342"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5941885.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9709..10122
                     /gene="msrB"
                     /locus_tag="OO332_001343"
     CDS             9709..10122
                     /gene="msrB"
                     /locus_tag="OO332_001343"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5941886.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10164..10436
                     /locus_tag="OO332_001344"
     CDS             10164..10436
                     /locus_tag="OO332_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5941887.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10806..11882
                     /locus_tag="OO332_001345"
     CDS             10806..11882
                     /locus_tag="OO332_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5941888.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11909..13288
                     /locus_tag="OO332_001346"
     CDS             11909..13288
                     /locus_tag="OO332_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941889.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13305..14350
                     /locus_tag="OO332_001347"
                     /pseudo
     CDS             13305..14350
                     /locus_tag="OO332_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14371..15207
                     /locus_tag="OO332_001348"
     CDS             14371..15207
                     /locus_tag="OO332_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5941890.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15212..16160
                     /locus_tag="OO332_001349"
                     /pseudo
     CDS             15212..16160
                     /locus_tag="OO332_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16170..17150
                     /gene="ydjG"
                     /locus_tag="OO332_001350"
     CDS             16170..17150
                     /gene="ydjG"
                     /locus_tag="OO332_001350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5941891.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17287..18045
                     /locus_tag="OO332_001351"
     CDS             17287..18045
                     /locus_tag="OO332_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5941892.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18163..19521
                     /locus_tag="OO332_001352"
     CDS             18163..19521
                     /locus_tag="OO332_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941893.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19614..20255)
                     /gene="pncA"
                     /locus_tag="OO332_001353"
     CDS             complement(19614..20255)
                     /gene="pncA"
                     /locus_tag="OO332_001353"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5941894.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20266..21282)
                     /gene="ansA"
                     /locus_tag="OO332_001354"
     CDS             complement(20266..21282)
                     /gene="ansA"
                     /locus_tag="OO332_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5941895.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21449..23305)
                     /gene="sppA"
                     /locus_tag="OO332_001355"
     CDS             complement(21449..23305)
                     /gene="sppA"
                     /locus_tag="OO332_001355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5941896.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23466..24017
                     /locus_tag="OO332_001356"
     CDS             23466..24017
                     /locus_tag="OO332_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5941897.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24134..25177
                     /gene="selD"
                     /locus_tag="OO332_001357"
     CDS             24134..25177
                     /gene="selD"
                     /locus_tag="OO332_001357"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5941898.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25182..27143
                     /gene="topB"
                     /locus_tag="OO332_001358"
     CDS             25182..27143
                     /gene="topB"
                     /locus_tag="OO332_001358"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5941899.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27270..28310
                     /locus_tag="OO332_001359"
     CDS             27270..28310
                     /locus_tag="OO332_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5941900.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28427..29770)
                     /gene="gdhA"
                     /locus_tag="OO332_001360"
     CDS             complement(28427..29770)
                     /gene="gdhA"
                     /locus_tag="OO332_001360"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5941901.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30006..30278
                     /locus_tag="OO332_001361"
     CDS             30006..30278
                     /locus_tag="OO332_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5941902.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30244..30651)
                     /gene="nudG"
                     /locus_tag="OO332_001362"
     CDS             complement(30244..30651)
                     /gene="nudG"
                     /locus_tag="OO332_001362"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5941903.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30738..31394
                     /locus_tag="OO332_001363"
     CDS             30738..31394
                     /locus_tag="OO332_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5941904.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ
                     "
     gene            complement(31403..32710)
                     /gene="ynjE"
                     /locus_tag="OO332_001364"
     CDS             complement(31403..32710)
                     /gene="ynjE"
                     /locus_tag="OO332_001364"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR5941905.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32777..33430)
                     /locus_tag="OO332_001365"
     CDS             complement(32777..33430)
                     /locus_tag="OO332_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5941906.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33430..34964)
                     /locus_tag="OO332_001366"
                     /pseudo
     CDS             complement(33430..34964)
                     /locus_tag="OO332_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34937..>35276)
                     /locus_tag="OO332_001367"
     CDS             complement(34937..>35276)
                     /locus_tag="OO332_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5941907.1"
                     /translation="ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFL
                     LSPDAQLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALE
                     QEWLRRYGTH"
BASE COUNT         8967 a         8333 c         9003 g         8973 t
ORIGIN      
        1 cgcggcaata ctctgattct gatacttatc ataaagaaag gaggagtctg ctttctgcac
       61 gatatagcgc agataggcac tcattgctct gtcgctggcg acaagttcaa aaataaggaa
      121 ggaagatgtc agcacaatga cacttgcggc gataaaatgc cggagcattc tatggtgcaa
      181 tttcatgata aatggcactc cttcggttag tgaaacgaga aaaccagata aagttcgatt
      241 gcggcctggc acaggggcgc taattatact catctctgcg ccgctgggga ggggaaattt
      301 aggggtaata gtaataaatt gtatgcaaaa gccacgcaaa aagcatggct ctgaatattt
      361 ttgcacttaa atattatgaa taatcacttt gttgcccccc tcgcttttcg cttcgcgtaa
      421 ggctttatct gcttccgtca tgacaagcga gatattacgc gtgtcacccg ttgaaaaaaa
      481 cgtgccaata ctgatggtca tttgttcagg aacatcatat ctgttactga agccggtcag
      541 gagttcgaca tttttacgca tacgttccgc aatgattttt gcctgggcac tattgagttc
      601 ggtaaatagc aagccaaaca cctcaccctc cagtcgcgct aaaatatcat cggggcggat
      661 gctttgctgg ataatatcga caattgaaac cagcacttta tcacccacac gatgtcccca
      721 ttgggcattg atgcgtttga actgattaat atcaaccagc atgacacaga agtccttaac
      781 aacaggtcgc gacaataatg actctacgct gttgaaaaag tagcgccgat tataaatatt
      841 ggtcagcaca tcatgaactg ccagtttgct ggagagttgc agttcctgaa aaatgttata
      901 aatcagaaaa gacacaacaa acagtttact ggagacttca atcccgcgac tgatgtacca
      961 gatggtgtaa ttatactcat ccagagtcag tagaagtaag ttgcagcata gcgatgccag
     1021 acataataat gctattaatg gccatagttc gctggctaat cgtgtacgca tgataataaa
     1081 gaacagtaaa aatgcccaca gacaaaccaa tatttttgta tagttgattc cccaggtcgc
     1141 agtatggttg tcaggacagt aatcgacgac atacaaagaa tagtcagcgt tataactgct
     1201 cagattgtgt gccagaaggg gaaaaactaa aaaaggaatc agcgccagca acagaatgcc
     1261 agtttttttc ttattgttgt caaggatgtt gtctttacca taacaaaaga gcgccaacga
     1321 ggttaaacaa ataaaactga gttggcggaa aatatagaaa atagaaacat cattcgactt
     1381 tgtctggatc agtgtactgt cgctgatagg tttatggata acaacaatag tttcaactaa
     1441 atagataagg ccacttaaaa gtgcaagact taaaatgagt gttggtagaa tttgtttgtt
     1501 agtgaaatat ttcattaaca tgaaaagaaa aatgattgcg ttaaggaaaa acaaagccac
     1561 aacgaccatc agataattca tttgtgggat gagttttaca ttcgtctttt cgttatataa
     1621 tgtggtgatg caaaataaaa ttaacgtcag caataaactg aaaataaaaa agctttgagc
     1681 ttttaatttt tgtgtgccca tagaaaccat aaaaaggaga gcaatgtgag tgctggattt
     1741 tattgatggc agagatatat agttttgacc aagatcaatt ttcgttaacg ataaaaaata
     1801 tttaaagaat aaccccaggg aaatacgtgt tgactgaatc atttttccga atgattgttt
     1861 tctggaacta aattgtgtaa aggttatgct gtcatattta aagcgattgt aagctaatgt
     1921 atgtaataaa tgagataatt tacagtaagc taaaaatcag ccaggcgata atgacccggc
     1981 tgatagtttt agtctttagc tgttgcattt tgtttatgaa acagttcacg gaacaccgga
     2041 taaatatcat cctggtcgcg gatgtgctgc atcgcaaagt tgtcgaaagt agattgcaga
     2101 tgctcatatt ctcgcacaca atgtctgatg tgcacgacgg gtaatttcga tatagctgta
     2161 ataacgaaca acaggtaata attttttcgc caggatttca tggcaaagcg gagagtcatc
     2221 ggcccagtta tcgccgtccg atgcttgtgc agcgtaaata ttccactgtg ccgggttata
     2281 acgctctttc actacctcat ccatcagttt cagggcgctg gaaacaatgg tgccgcctgt
     2341 ttcctgcgag tagaaaaact catgttcatc gacttctttc gcctgggtat gatggcggat
     2401 gtatacgact tccacgttct tatacgttct gctgaggaac agatacagca gaatataaaa
     2461 acgcttagcc atatctttag tggattgatc cattgaaccg gaaacgtcca tcaggcaaaa
     2521 catcactgcc tggctggagg gatcgggccg cttctcgtag ttcttgtaac gtaaatcgaa
     2581 ggtgtcaata aaagggacgc gttcaatttt ggcacgtaat tctgcaattt ctttgcgcag
     2641 acgttcctct tccagcagct gcgcaggttc actgttgctg atgatggcca aattctcttc
     2701 cagtgcatga agttcccgcc gcttgcctgc cgtcatggct gtgcgtcgcg ccagtgagtt
     2761 ctgcaaggaa cgcacaacgc tgatattggc tggaacgccg ttagcggtat aacccgcccg
     2821 atgcgtttta tattcggtca gctggcgttg ttggttttgt ttcagattcg gtaaggccaa
     2881 atcttcaaag agcagatcaa gatactcatc tttcgaaatc tgaaagacaa attcatcctg
     2941 accttcacca tcctggctgg cctggccctg accactgccg gaacctccgc cgccaccctg
     3001 gggacgttca attcggtcgt tctgaacgaa atggtcattg cccggatgca cgcggtggcg
     3061 cagaccgcca cgcccctgat gaaacattgg ttcgctaata tcttccgtgg gaatggatac
     3121 ggattcgctg ctgtcgacgt cagtcaccga acgcttatta atggcctcgg agatcgactg
     3181 tttaatttgc gctttataac ggcgtaaaaa acgctggcga ttcaccatgc ttttgttttt
     3241 gccgttcaga cgccggtcaa taaaccaggt catatgcccc ccgtactaca tttgccaact
     3301 gtatgctacg cctgtgttgc cgcttctcag gcccacggtt cgctatcgtt gtaggcccgg
     3361 tagcatgtgt accgggccag ggttgttaag acgatttacg tacgcgcaaa taccattcgc
     3421 acagtaaacg cacctgttta cgggtgtagc ctttttccat catacggtcg acaaagtcgt
     3481 cgtgtttctt ctgctcgtcg gttgacgttt tggcgttaaa cgagataacc ggcaacagct
     3541 cctcggtatt ggagaacatt ttcttctcga taaccgtgcg cagtttttca tagctggtcc
     3601 agttcggatt gcgtccgctg ttattcgctc tggcgcgcag tacgaagtta acaatctcgt
     3661 tgcggaaatc ttttggatta ctgatccccg ccggtttctc gattttctcc agctcggcgt
     3721 taagagactc gcggtcaaac agctgcccgg tatccggatc gcgatactcc tgatcctgaa
     3781 tccagaaatc cgcgtaggta acataacggt cgaaaatgtt ttgcccatat tcggaatagg
     3841 attcaaggta ggccgtctgg atctctttgc cgataaactc ggcatatttc gggatcagat
     3901 aacctttcag gaactccaga tagcgttctg cctgctcttg cgggaactgc tcgcgctcga
     3961 tctgctgttc caggacgtag aacagatgga ccgggtttgc cgccacttct acatgatcga
     4021 agttgaacac gcgggggagg atcttaaacg caaaacgcgt cgacagaccg ttcatccctt
     4081 catcgacacc ggcgtagtca cgatattcct gatacgactt ggctttcgga tcggtgtctt
     4141 tcagactttc gccatcataa acccgcatct ttgaataaat gctggagttt tctggctctt
     4201 tcaggcgcga aagaatggaa aaacgtgaca gtgtttcgag cgtgccaggg gcgcatgggg
     4261 cgtgagtcaa ttcactgtga ttaagcaatt tctcgtagat tttgatctct tcggaaatgc
     4321 gcaagcaata cggcaccttc acgatgtaaa cacggtcgag gaaggcttcg ttgtttttgt
     4381 tattacggaa agtgacccat tcggactcgt tcgagtgggc gagaataatc ccgttgaacg
     4441 gcagggcgga gataccttcc gtcccgttgt agttatcttc ctgagtggcg gttaacaagg
     4501 gatgcagcac ttttatcggt gctttaaaca tctcaacgaa ttccatgatc ccctgattgg
     4561 cgcggcacag cgtaccggaa tagccgtagg cgtccgggtc attctgcgcg tagtgttcga
     4621 gtttacgaat atcgactttc ccaaccagcg cggagatgtc ctggttgttc tcatcaccgg
     4681 gttccgtttt ggcgatagca atttgttgca gaattgacgg ccagaccttc actacccgga
     4741 acttagtgat atcgccacca aattcatgca ggcgttttgc cgcccatggc gacatgatgg
     4801 tgccgagata acggcgagga atgccatact ctttttccag aatctgagca tcttcctgcg
     4861 gattgaaaag acagaacgga tgatcgttga ccgggctacg ctcaccgttg gcgctcaata
     4921 cataaatcgg tacgagctgc attaatgatt tcagtcgctc agcaagcgat gatttacccc
     4981 cacccacagg ccccagcaga tacaggattt gtttcttctc ttccagcccc tgagccgcgt
     5041 gtttcagata agagacaatc tgttcaatcg cgtcttccat gccgtaaaac tcttcaaatg
     5101 ccggataacg tgcaatgacc cggttagaaa agagtcgaga aagtctgggt tcctgggctg
     5161 tatcgaccat gacaggctca ccgatagcca tcaatagccg ctcagccgcg ttggcataag
     5221 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg
     5281 cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt
     5341 tttagcacag gttaagagcc gttcatatga atagtagagg ctcccggaag aggtaaactg
     5401 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttatcgacg atgagaacaa
     5461 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
     5521 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
     5581 taaagaccag gctcgccagt aacgctaaat ccatttggct gtaagcgcgg tgtcatccgc
     5641 ggcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt aaaaaggttg ggtttcgatg
     5701 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
     5761 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
     5821 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gagcacccat ataaagatta
     5881 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
     5941 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gataaacttt caattaccgc
     6001 ttactggtcg ccgctttact tcaaagcgaa agacagtggc gatcaccaaa tgcgtcacct
     6061 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
     6121 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca ttgtctggga
     6181 tatggcttgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt
     6241 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     6301 tcgcagcggt ctgcgtgggt ataacccaaa tgacagctgg agcccttacc ttgagctgag
     6361 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct
     6421 gtctgatgaa gttactgaca gcccgatggt ggataaatcc tggactggcc tgatttctac
     6481 cgggatcacc tacaaattct gataatgcat ctttccaggg cattttgtgc ataaaaatag
     6541 ggcgtcacgc cctgttttgc attacggtgg tgcaatacct gggaggaaac aatgcaacaa
     6601 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     6661 atgggcgaag atgccagtca gcgcaaaaca gaagttgctg cactacgcgc aggcattgaa
     6721 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaggtg
     6781 gttggggaag cattaaccgg tctgcgagag aaggtctttc tcgtctctaa agtctatccg
     6841 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     6901 actgattatc tcgatctcta cttattacac tggtctggca gtttcgcttt tgaagagact
     6961 gttgctgcga tggaaaaatt gatcgcccag ggaaaaatcc gtcgctgggg cgtttctaac
     7021 cttggttatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     7081 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact cccctggtgc
     7141 cagcaacagc agatgccggt gatggcttac agtccattag tccaggccgg gcggttgcgc
     7201 aatggactgt taaaaaacgc ggtagtcaac gaaattgcac atgtccaaca aaatgcagct
     7261 gcgcttgagg tcgaactttc gtcagcggaa ttagctatgc tggataaggc atatccggca
     7321 ccaaaaggaa aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac
     7381 ccggcacaca acattaacgc aaattaacgt ttggcaacgc gaatggattg cgccagatgt
     7441 gcaggtttct ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca
     7501 aaggtcttgt agccatcgtc cggcatgtcg cccatgctaa ctgaaagcgc cggtcccggg
     7561 ttccagccga caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct
     7621 tcatcattaa tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg
     7681 aaggtctgaa taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag
     7741 cgatcgccca gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc
     7801 agggcagagg tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc
     7861 acgcggaaat gcgctaacag cgtaaagtcg tgcggccaga attttttcgt ctcttcgctt
     7921 tgtgtcaatt caaaagtcag cgctacgcca tcagcatctt catgatgtga tttcagcgtc
     7981 cacggcaggt tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac
     8041 cacggccagc aaaccggtac gctaccgcga atagcgacgc catttttgaa cggtgtgttg
     8101 ttgctcaacc acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc
     8161 tgtaatgcaa aagaggcttt tacctgggga tgatcgacca caatgaggtc cagttcatcc
     8221 agtttacgac gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc
     8281 ttaatcatga cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt
     8341 cgctcttttt agatcacagt gtcatctcaa cttatttgga gatgtgagcg atcaggtcca
     8401 gaactttgtt ggagtaaccg gtttcgttgt cgtaccaaga taccagtttc acgaagttgt
     8461 cgttcagagc gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat
     8521 cggtagatac tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag
     8581 cagctttaac ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca
     8641 ggtcaactac ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat
     8701 tcagttctgg cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt
     8761 tctgggaagc gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct
     8821 gagtagcggt agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt
     8881 tgataacttt agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt
     8941 cctggccagc atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag
     9001 acggaccagt cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt
     9061 cagtcaggaa cagaccagtt gcttcagcga caacgtcaac acccacttcg tcccatttca
     9121 ggttagccgg atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac
     9181 cgtctttcac ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt
     9241 atgccatgta atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt
     9301 tctgagcagc acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga
     9361 tagtcatata ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa
     9421 aaccttacgc agcgtcaagc ggaatcgtgt caatcattgc gacagatcaa tcctgtgcct
     9481 aagcattacg cgactgactc gcctcactct tcctttgggc ttgagaccac atggggtcgg
     9541 cgccccgaat tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc
     9601 agccatttcg tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt
     9661 tagaatcagt cacgtaatgt gagcacgatt aaagtgagat gtgagtaaat ggcgaataaa
     9721 ccttcggcag aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat
     9781 catgggacag aaccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat
     9841 cactgtttga tctgcgatgc cccgctgttt cattccgaaa ccaagtatga ttccggctgt
     9901 ggctggccca gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg
     9961 tcacatggaa tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat
    10021 gtctttcccg acgggccgca accaacaggc gaacgttatt gtgttaactc tgcttcttta
    10081 cgcttcactg atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact
    10141 tattccacag gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg
    10201 aggtatacca gcgtttgtcg accgctgttg aactggggaa atggcctgat ggcgttgcgt
    10261 tgaccgagga acaaaaagag aattgcctgc aactggtgat gctgtggcaa gcccgccata
    10321 ataccgaagc acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac
    10381 agcagttaaa agaagatttt ggtatctcag caaagcctat tgccatgttt aagtaagtgg
    10441 ttcatcccct ttttgccgcg gctatgaagg ggatagccaa caatgacccc acgactcgtc
    10501 tcaattatct cgacccgacc tggctaagtc ccagtccgat ctcttgcagt cgcgttcacg
    10561 cctgaatgac ttctcctgaa cttaagtctc agacctattt ggccggtaat ccctctcgaa
    10621 tttgctcttt ttgcgtcccg ccttccacca acctgtaaca gacccagcca ttttctgcgt
    10681 aattgtgatc taagttgtaa aatgcaggtc attatctgtg ttgtgcagat ttaatgttga
    10741 cctcaaaaat catgtgggta agtatgatgg ccatctaagc aaaacaaaac acagaggatg
    10801 acgcaatgaa agcactggct cggtttggca aggcctttgg cggctacaag atgattcatg
    10861 tcccacaacc catttgtggc ccggaagacg tagtgattga aattaaagcc gcggcaatct
    10921 gcggcgcaga catgaagcac tacaatgtcg atagcggttc tgatgagttt aactctatcc
    10981 gcggccatga gttcgcaggt tgtattgcgc aggttggtga aaaagtcaaa gactggaaag
    11041 tggggcaacg cgtcgtgtcg gataacagcg gtcacgtttg cggcgtttgt ccggcctgtg
    11101 aacagggtga ttttctgtgt tgtacagaaa aagtaaacct tggtctggat aacaatacct
    11161 ggggcggtgg tttttccaaa tattgtctgg ttcctggtga aattctcaaa attcatcgtc
    11221 atgcgttgtg ggaaatccct gatggtgttg attatgagga cgcagccgta cttgacccta
    11281 tctgtaatgc ctataaatcc atcgcgcagc aatcgaaatt ccttcctggt caggatgttg
    11341 tcgtcattgg cactggccca ctcgggctgt tttccgtaca aatggcgcgg attatggggg
    11401 cggtaaatat cgtcgtcgtt ggtctgcaag aagatgtggc ggtccgcttc ccggttgcaa
    11461 aagaactggg ggcgacggca gtagtaaatg gttctaccga agatgtggtg gcgcgctgcc
    11521 agcaaatttg tggcaaagat aatctgggac tggtgattga atgctccggt gccaatatcg
    11581 cactgaaaca agccatcgaa atgctccgcc cgaatgggga agtggtacgc gttggaatgg
    11641 gcttcaaacc tcttgatttc tcgattaatg acattaccgc ctggaataaa agcatcattg
    11701 ggcatatggc ctatgactcc acctcatggc gtaacgctat ccggctatta gccagcggcg
    11761 ctatcaaagt caaaccgatg atcacgcatc gtatcggcct gtcgcaatgg cgcgaagggt
    11821 ttgatgcgat ggtcgataaa accgcaatca aagtgatcat gacttacgac tttgatgaat
    11881 aactactctc ctgaaggaac gttatcgcat ggaacagata acaaaaccgc attgtggtgc
    11941 acggctggat cgcttaccgg attgccgctg gcattcgtca atgtttgcca tcgtcgcgtt
    12001 tggcttgctt gtctgctgga gtaatgccgt tggtggcttg atcctcgcgc agctgaaagc
    12061 gttgggctgg acagataatt ccaccacagc cacattctca gcaatcacga ccgccggaat
    12121 gtttctcggt gcgctggtgg gcggcatcat tggcgacaaa accggtcgca gaaatgcgtt
    12181 catcctctat gaggccattc atattgcctc gatggttgtc ggtgctttct caccgaatat
    12241 ggattttctt attgcctgcc gttttgtgat ggacgttggg ctgggagctt tactggttac
    12301 actgtttgct ggtttcaccg aatatatgcc cggtagaaat cgcggaacgt ggtccagtcg
    12361 ggtttccttt attggcaact ggtcatattc gctctgctca ctgattgcga tgggactcac
    12421 gccgctgatt agtgcagagt ggaactggcg ggtacaactg cttattcctg caatattgtc
    12481 gcttatcgct acggcgctgg cctggcgcta ctttcctgaa tccccgcgct ggctggaatc
    12541 gcgcggacgg tatcaggaag ccgagaaagt catgcggagt atagaagaag gcgtcatacg
    12601 ccagacgggt aaacctttgc cgcctgtggt tattgctgat gacggtaaag cgccacaagc
    12661 ggtgccgtat tcagccttac tgacaggagt attactgaaa cgcgtgatat taggctcttg
    12721 tgtgctgatt accatgaacg ttgtgcagta cacactaatt aactggttgc caacaatatt
    12781 catgacccag gggattaatt taaaagactc gattgtttta aataccatga gtatgtttgg
    12841 cgcgccattt ggtattttta ttgccatgct ggtgatggat aaaattccgc gtaaaacaat
    12901 gggtgtgggg ctattaatcc tgattgcggt gctcggatat atctattcac tgcaaaccag
    12961 tatgttgctc ataacgctga ttggtttctt cctgattact ttcgtctata tgtacgtttg
    13021 ctatgcctcg gcagtgtatg tccctgaaat ctggccgaca gaggccaaac tccgtggctc
    13081 cggtctggcg aatgcggtag ggcgaatcag tggtattgcc gcaccttatg ccgttgcagt
    13141 gctgctcagt agttatggtg taacgggagt ctttattctt ctgggggctg tttcaattat
    13201 tgtcgcaatt gctatcgcca ccattggaat tgaaaccaaa ggtgtctccg ttgaaagttt
    13261 aagtattgat gcagtagcca ataaataata cgggtggatt caaaatgaaa aattcaaaag
    13321 caatattgca ggtgccgggc acaatgaaaa ttatttcagc agaaatacca gtgcctaaag
    13381 aagatgaagt tttgattaaa gtagaatatg tcggtatttg tggttcagat gtacatggtt
    13441 ttgaatcagg cccgtttatt ccgcctaaag acccaaatca agaaattggc ctgggtcatg
    13501 aatgcgccgg gacggttgtg gctgtgggaa gccgcgtgcg caaatttaaa ccgggggatc
    13561 gggtaaatat cgaacctggc gttccttgcg gtcactgtcg ttactgtctg gaaggcaaat
    13621 ataacatctg cccggacgtt gattttatgg cgacacaacc caactaccgc ggcgcattaa
    13681 cgcactatct gtgtcatccg gagagcttta cttacaaact gcccgacaat atggacacga
    13741 tggaaggggc gctggtggag cctgccgcag tcgggatgca tgccgcgatg ctggcagatg
    13801 ttaaaccggg taagaagata attattctgg gagcaggttg tattggtttg atgacgttgc
    13861 aagcgtgcaa atgcctgggc gcaacggaaa ttgccgtcgt tgatgtactg gaaaaacgtc
    13921 tggcaatggg ggaacagctt ggtgcgacag tggttattaa tggcgcaaaa gaggacacta
    13981 ttgcacgctg tcagcaattt accgaagaca tgggcgcaga tattgttttc gaaacagcgg
    14041 gttctgcggt caccgttaaa caggcacctt taatctggta atgcgcggcg gtaaaattat
    14101 gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca atcgcgaagt
    14161 cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg aagctatttc
    14221 ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc gggatgtaca
    14281 acaggcattt gaagagtcag ttaacaacaa gcgcgacatt attaaaggcg ttattaaaat
    14341 tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata ttgggaaaac
    14401 gatgccacaa ataaacatta tgcaattgcc cattttaacg tatggaatgc agaaatgttg
    14461 atgggcgtta aagacgcagc cgaagaagcg aaatccccgg ttattatttc ttttggtaca
    14521 ggttttgtcg gtaacacctc atttgaagat ttctctcaca tgatggtatc aatggcacaa
    14581 aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtatgga gattattcat
    14641 aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt tgatttcgaa
    14701 gaaaatattc gtttaaccaa agaggctgtt gatttctttc atccgctggg tattccggta
    14761 gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct ggcgggctat
    14821 cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg tgactcattg
    14881 gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccgcaatt gaactttgag
    14941 gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg cgcatcgggg
    15001 atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat caacattcat
    15061 acggagctct gtcaggccgc aatggtcgca gtgaaagaaa accaggatca gcctttcctg
    15121 catttagaac gtgaagtacg taaagctgta aaagaacggg cattggaaaa aattgaactg
    15181 ttcggttctg atggcaaagc ggaataatga tatggataat ctcgacgtta tttgtatagg
    15241 tgccgctatt gttgatattc cattacaacc ggtcagtaaa aatatctttg atgtagattc
    15301 ttatcctctt gaaagaatcg caatgagcac cggggggcga tgcaataaat gaagcaacaa
    15361 ttatttctcg tctggggcat cgtaccgcat taatgagccg cattggtaaa gatgcctcag
    15421 ggcaatttat tctcggccat tgtcgcaaag agaatattga tattcaaagc ctgaaacagg
    15481 acgtcagcat agatacatct attaacgttg gactggtgac ggaggatggc gagcggacgt
    15541 ttgtcacgaa ccgtaacggc agtttgtgga aattaaatat tgacgatgtt gattttgcgc
    15601 ggtttcctca ggcgaaatta ttatcactgg ccagtatttt caacagtcct ctattggatg
    15661 gtaaagcgct aacagaaatt tttacgcaag cgaaagcccg gcagatgatc atttgtgccg
    15721 atatgatcaa accgcggttg aatgaaacgc tggatgatat ttgcgaagca ttgagttatg
    15781 tcgattatct gtttccaaat tttgccgagg caaaattact cacagggaaa gagacactgg
    15841 atgaaattgc tgactgcttt cttgcgtgcg gcgtaaaaac ggtggtgatt aaaacgggta
    15901 aagacggctg ctttatcaag cgtggtgaca tgacgatgaa ggtgccggcg gtcgcaggaa
    15961 taaccgccat cgacaccatt ggcgcgggcg ataactttgc ttcaggtttt attgcggcac
    16021 tgttagaagg caaaaatctg cgtgaatgcg cacgctttgc caatgcaacg gcggctatct
    16081 cggttctaag cgtcggtgcc accaccggcg taaaaaacag aaagctggtc gaacaattgc
    16141 tggaagaata cgaaggataa tgaaggcaaa tgaaaaagat acctttaggc acaacggata
    16201 taacgctttc gcgaatgggg ttggggacat gggccattgg cggcggtcct gcatggaatg
    16261 gcgatctcga tcggcaaata tgtattgata cgattcttga agcccatcgt tgtggcatta
    16321 atctgattga tactgcgcta ggatataact ttggcaatag tgaagttatc gtcggtcagg
    16381 cgttaaaaaa actgccccgt gaacaggttg tagtagaaac caaatgcggc attgtctggg
    16441 aacgaaaagg aagtttattc aacaaagttg gcgatcggca gttgtataaa aacctttccc
    16501 cggaatctat ccgcgaagag gtagaagcca gcttgcaacg tctgggtatt gattacatcg
    16561 atatctatat gacgcactgg cagtcggtgc cgccattttt tacgccgata gctgaaactg
    16621 tcgcagtgct taatgagtta aaggctgaag ggaaaattcg cgctataggc gctgctaacg
    16681 tcgatgctga ccatatccgc gagtatctgc aatatggtga actggatatt attcaggcga
    16741 aatacagtat tctcgaccgg gcaatggaaa acgaactgct gccgctatgt cgtgataatg
    16801 gcattgtggt tcaggtttat tccccgctag agcagggatt gttgaccggc accatcactc
    16861 gtgattacgt tccgggcggc gctcgggcaa ataaagtctg gttccagcgt gaaaacatgc
    16921 tgaaagtgat tgatatgctt gaacagtggc agccactttg tgctcgttat cagtgcacaa
    16981 ttcccactct ggcactggcg tggatattaa aacagagtga tttaatctcc attcttagtg
    17041 gggctactgc accggaacag gtacgcgaaa atgtcgcggc actgaatatc aacttatcgg
    17101 atgcagacgc aacattgatg agggaaatgg cagaggccct ggagcgttaa atactcaaca
    17161 cgattcacct ctgtttctgt gaaaactgtg atgaaaatct gtgattcaaa taggttattt
    17221 tgaaagtaaa catcggttat gcgataatcg cgctaatgtg atgtaaaaag atacaggggt
    17281 gtaatcgtgg cggcaaaaga caggattcag gcaattaagc aaatggttgc caacgataaa
    17341 aaggtgacag tctcaaattt gagtgggatt tttcaggtaa ccgaagaaac cattcgccgc
    17401 gatcttgaga agctggaaga tgaaggcttt ttgaccagaa cctatggtgg tgctgtttta
    17461 aatacagcgg tgttgacgga gaatatccat ttttataagc gcgcttcatc gttttatgaa
    17521 gagaagcagc ttattgcacg caaggcacta ccctttatcg acaataaaac caccatggca
    17581 gccgattcca gtagtaccgt aatggaattg ctcaaattgt tacaggaccg tagtgacctg
    17641 acgttgctaa ccaactcggc ggaagcgatt catgttctgg ctcagtcaga aattaaagtc
    17701 gtttcaacgg gtggggaact aaacaaaaat acactttccc tgcaaggaag aattactaaa
    17761 gagatcatca gccgctacca tgtcgatatc atggtaatga gttgtaaagg tcttgatatt
    17821 aacagcggcg cgctggactc taatgaagcg gaagctgaaa tcaaaaagac aatgatccgt
    17881 caggcgacag aagttgcgtt attggttgat cactctaagt ttgatcgtaa agcttttgtc
    17941 cagttagctg actttagtca tattaattat ataataactg ataaatcacc gggtgcagag
    18001 tggatagcat tttgcaaaga caataatatc cagctggtgt ggtaatttaa gcacacgttg
    18061 tcaatattat ttgttcctgc gcctggaaat aaaatgatga taaagatgcg gcaagtgaat
    18121 ttgccgcaat tgctaccttt tatagcaaat ccgaggcacc caatggaaca atatgatcaa
    18181 attggcgcaa gactggaccg cttgcctttg gcccggtttc attatcgtat atttggtata
    18241 ataagcttta gtctgttatt aacggggttt ttgagttact caggtaatgt cgtcttagca
    18301 aagctggtaa gcaatggatg gtcaaataat ttcctcaatg ccgcctttac ctcggcatta
    18361 atgtttggtt atttcatcgg ctcacttact ggtgggttta ttggtgacta ctttgggcgg
    18421 cgcagggcgt ttcgcataaa tcttctcatc gtcggtattg ctgcaacagg ggccgctttt
    18481 gtccctgata tgtactggct cattttcttg cgcttcctga tgggaacagg aatgggggcg
    18541 ctgattatgg ttggctatgc ctcatttacg gagtttatcc ccgcgacggt gcgtggaaaa
    18601 tggtccgcgc ggctctcatt tgttggtaac tggtcgccca tgctgtctgc ggcgataggc
    18661 gtggtggtta tcgctttttt tagttggcga ataatgtttc ttctgggtgg tattggcata
    18721 ctgttagcct ggtttctctc aggtaaatac tttatcgagt cgccacgatg gctggcaggg
    18781 aaagggcaaa tcgccggtgc agaaagccaa cttcgtgaag tagagcagca aattgaaagg
    18841 gagaagagta ttcgtttacc ccagcttact ttgaaccaga gcaacagcaa ggttaaggta
    18901 atcaagggta ccttcaggct cctgtttaaa ggggaaatgt tacgacgtac attagttgcg
    18961 ataactgttt taattgcaat gaacatttcg ctttatacca ttaccgtatg gataccgacc
    19021 atatttgtta actccggcat tgatgtcgat aaatcaatat taatgaccgc tgttattatg
    19081 attggtgctc cggtaggaat atttattgcg gcattaatta ttgatcattt tcctcgtcga
    19141 ttatttggct ccgccttact tattattatt gccgtgttag gctatatcta ttcaagtcag
    19201 actacagagt gggcgatttt aatctatggt ctggtgatga tcttcttttt atacatgtat
    19261 gtttgcttcg cgtcggcggt ttatatcccg gagctttggc caacgcattt acgcctgcgc
    19321 ggttcgggtt tcgttaatgc cgtcggacgg atcgtcgcag ttttcacgcc ctatggcgtt
    19381 gcggcattat taacacatta tgggtcgatc acggtgttta tggtgcttgg tgtcatgtta
    19441 gtgctctgtg cgctggttct ctccattttt ggcatcgaaa cgcggaaggt gtcgctggaa
    19501 gagatttctg aggtgaatta aatctttcgt tgatggcatc aattgccaga tgtaccactg
    19561 acgtgtttta tttggcctac aatgccagta cagaattgta ggccagcgta aaattacccc
    19621 tgcgtctctt cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata
    19681 aacgcgtgtg cactgtcctg gggctggata ttcacgccac gacagccatc ggtaatcacg
    19741 tttaccttat aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta
    19801 gccaggccca taacgatcaa ttcatcgatt tcatgatcgc gcaaccagtc atcaagcgcg
    19861 gttttctgcc gacgtccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg
    19921 cctttatgga acaccgctgc gatctctttt tgattcagta acggatgtaa ttgtgcgcct
    19981 tcactgttct gcacacagtg ttcaggccag aaagtttgcc gcaggccgtc gagttggcct
    20041 ggcgtataag gctctacgcc gtgctggctg gcaaagctgc cgtgattcgc cggatgccag
    20101 tcctggctgg cgataaccgc ttctccgcgc gactggcacc agtcaataag gcggttagca
    20161 acctccaccg tactgtcacc ttccggtacg gcgagcgcgc caccagcaca gaaatcattt
    20221 tgtaaatcga ccagtaacag ggcgcgatgg ggcattgcag tctccttaat catccagcgt
    20281 cagttcgccg cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg
    20341 gctcagcagg taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat
    20401 aacgccggca tgggcgagga cgttaccggt ggcataacca ctcatattca ctttaccgga
    20461 catacattgt gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg
    20521 caggaaggct ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt
    20581 caccggttgg cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac
    20641 tacgccaatt ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc
    20701 gggtggcgta ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg
    20761 agaggcaaac gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa
    20821 tcggttattg aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc
    20881 attcagcaga ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt
    20941 cacaatgacc ggtttaccga gattctcgag catgaacgac agcgcagagg cggtatacgc
    21001 catcgtgtcg gtaccgtgta gaatgacaaa accatcatag tcgtcatagt gcgctttaat
    21061 atcttcagca atatgctgcc agtcttccgg cgtcatatca gaagaatcca tcaacggcgt
    21121 atattcatga atggtgaaat ctggcatctc caggcgatgg aattccggca tcagcgccag
    21181 ttggcgttgt agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat
    21241 ggtcccgccc gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat
    21301 atcgaaagag caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata
    21361 aggaaatatg gggcgagaca gcgggaagag gattcgtcga ggtaaaaaat aatgtggccg
    21421 acgatctgtc ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg
    21481 cataacggtt ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag
    21541 aggctaccga ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc
    21601 ggacagaacc agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt
    21661 attccagatg ccactgtttc acttttgcga gctctgctgc tttggcaacc gcatcatcga
    21721 aatccccgag actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc
    21781 cctgggcgat tttatcaatt tgctccggcg tcgaatgacg cgcatcagca accagcgtga
    21841 taaagcgttt atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca
    21901 gtgccctggt gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac
    21961 caatcgaatc cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg
    22021 tcagggtgct ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag
    22081 atgccgccat gccgcccatc gatacaacca cagacttacc cgctgcccgg gctgctgcca
    22141 gttcagcgcg aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga
    22201 caatcgcttt cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac
    22261 cgccaacatt cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac
    22321 cgatgctgtc accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc
    22381 gataattttt atcagtctta ctccagccga actctttggt cagagctttt tcgatttcgg
    22441 cactcgatgc cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac
    22501 cggttttggt taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga
    22561 tctgccggtt agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc
    22621 tgtcagcttc gcgggctgcc ggtgacatat catcacgaat aaacggttca acggcagatt
    22681 tatacgtacc cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt
    22741 agtacagacc gttggtagca aaaccgtgca gatcaaccac gccttgcggt gacagccaaa
    22801 ttttattggc gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat
    22861 aaaccggttt cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg
    22921 gctggtcgcc gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt
    22981 ccttcgcctg gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg
    23041 aactggcacc aagcagctgg cggcttaatt tactaaaccg ctgagaactg tctggtttat
    23101 cgacgatcac accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat
    23161 caccaccact gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca
    23221 ggttaagtac catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg
    23281 caataaatcg ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg
    23341 cgcaatagat gtggttatcg taatgaccca accggcactt gtcagcagga attgcggact
    23401 gtgctgtaac aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta
    23461 atcaaatgga tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ttggctgaac
    23521 ccgcgccaac gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc
    23581 ataagtccat gcaaccgtgg catttttttg tgattgaagg ggaagggtgc gagcgtttca
    23641 gcgccgtact ggaacagggg gcgattgctg ccggtagtga tgataaagct atcgacaaag
    23701 cccgtaatgc gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcaaagaaa
    23761 atcataaagt cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc
    23821 aaatggcagc cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa
    23881 gtccggtagt gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct
    23941 acctcggcac gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt
    24001 ttgtaactta tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga
    24061 attcagacac tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg
    24121 acaggagatg tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg
    24181 ttgcggctgt aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa
    24241 gtttgttgat ccgaatttgc ttgtgggtaa tgaaacccgc gacgatgcgg cggtgtacga
    24301 tctgggcaat ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa
    24361 tcctttcgat tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg
    24421 gggcaaaccg attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga
    24481 aattgcccgc gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct
    24541 ggctggcggt cactccatcg atgcgccgga gccgattttt ggtctggcgg taacggggat
    24601 cgtaccgacc gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct
    24661 gacgaaaccg ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgttgaaacc
    24721 agaacatcag ggactggcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt
    24781 tgccaacatc gaaggcgtaa aagcgatgac cgacgttacg ggctttggtc tgctgggcca
    24841 cttgagcgaa atgtgtcagg gggctggtgt gcaggcacgc gtcgactatg aagcgatccc
    24901 gaaactcccc ggtgttgaag agtacattaa gttgggcgca gtacctggcg gcactgaacg
    24961 taactttgcc agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatctgct
    25021 gtgtgatccg caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga
    25081 ggttaaaact acagccgccg agtttggcat tgaactgacg gcgattggcg aactggtgcc
    25141 agcgcgcggc ggtcgtgcca tggttgagat ccgttaattc aatgcggttg tttattgccg
    25201 aaaaaccgag tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg
    25261 atggctttat cgagtgcggt aatggtcagg tggtgacttg gtgtatcggt cacctgcttg
    25321 agcaggcgca gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc
    25381 cgattgtccc ggaaaagtgg caattacagc cacgaccctc cgtgaccaaa caacttaacg
    25441 tcatcaaacg cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg
    25501 aagggcaatt gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc
    25561 agcaggtgca gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgatcg
    25621 accgtcttcg ttccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc
    25681 gtgccgactg gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg
    25741 ccggttatca gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg
    25801 tgcgccgcga tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac
    25861 atatcgtgac acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg
    25921 aaccgtacca ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta
    25981 accgcattag tggtcaaccg gctattgtca ccagctataa cgataaacgg gaatcagaat
    26041 ccgcgccgct gccgttttcg ctttcggcgt tgcagattga agcggcaaaa cgcttcggtc
    26101 tgagcgcgca gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca
    26161 cttatccgcg ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg
    26221 tgatgaatgc catcagcgtt catgcaccgg atctgttgcc gcagccagtg gtagatccag
    26281 atatacgcaa ccgctgttgg gatgacaaaa aggtcgatgc gcaccacgcc atcatcccga
    26341 ccgcacggag ttctgcgatc aacctgacgg agaacgaagc gaaggtctat aacctgattg
    26401 cccgtcaata cctgatgcag ttctgcccgg atgcggtgtt ccgcaagtgt gttatcgaac
    26461 tggacattgc caaaggcaaa tttgtcgcta aagcccgttt tcttgctgaa gcaggctggc
    26521 gcacgctgtt aggcagcaaa gagcgcgatg aagaaaacga cggcacgcca ttgcctgtgg
    26581 tggcgaaagg cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc
    26641 cgccgcgcca ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg
    26701 tgcaggacaa agatctgaaa aaggtccttc gtgcgaccga tggtctgggg acagaagcaa
    26761 cccgtgccgg gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct
    26821 atatccactc caccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc
    26881 gaccggacat gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc
    26941 gctatcagga ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca
    27001 aacgtacgcc ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg
    27061 ataagaaaaa ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag
    27121 ccggaagcgg ggcgatagcg taagcgagcg aatctttcgt gctattcgag tcatattctg
    27181 aaatatccag cggatcaaga aaattcgttg gatatttttt tgcatggata aaattatcgc
    27241 ctctaaagta tgtaataaca gggaatgtga tgaaaaaagt tttattacaa aaccatcctg
    27301 ggagcgagaa gtattctttt aatggctggg gaatatttaa tagtaatttt gaacgggtga
    27361 ttaaagaaaa taaggccatg ctgctttgta agtgggggtt ttatttaaca tgtgttgtcg
    27421 ctgtaatgtt tgtattcgca gcgataacat ccaacggttt gaatgaaaga ggcctgatta
    27481 ccgcgggatg ctcttttctt tatctattaa ttatgatggg gcttattgtt cgggccggtt
    27541 ttaaagcaaa aaaagaacaa ctacattatt atcaggctaa aggtattgag ccgctcagta
    27601 tcgagaagtt acaggcgcta caattgatcg caccttatcg attctatcat aagcaatggt
    27661 ctgaaacgct agagttctgg ccgcgaaagc ctgaacctgg caaagatacc ttccaatatc
    27721 atgtgcttcc ttttgactcg atcgatatca tcagtaaaag acgcgagtct ttagagaatc
    27781 aattgggtat cgaagatagc gaaagttatt gtgccttaat ggagcatttt ctttctggcg
    27841 accatggagc caataccttt aaagcaaaca tggaggaagc cccagagcag gttatcgccg
    27901 tgttgaataa atttgctgtt tttccctcag actatatctc tgattgcgct aataatcgct
    27961 ccggtaaatc ctcggcgaag ctaatatggg cggcggaatt atcatggatg atctcgatat
    28021 caagcacagc ttttcaaaac gggacaattg aagaagaact ggcctggcat tatataatgc
    28081 ttgcttctcg aaaggcgcac gagttgttcg aaagcgaaga agattatcaa aaaaatagtc
    28141 taatgggatt tctttactgg catatctgct gctatcgcag aaagttaacg gatgctgaac
    28201 ttgaagcgtg ttatcgttac gacaagcagt tttgggagca ctacagtaag aaatgccgtt
    28261 ggcccataag aaatgttccc tggggagcat catccgttaa atactcataa acgcctgaaa
    28321 ttttgccggg ggcgctgcgc ttgcccggcc tacattagag cgtaactgca atgaattgtg
    28381 cccatctgta ggcctgataa gcgtagcgcc atcaggcatt tacaacttaa atcacaccct
    28441 gcgccagcat cgcatcggca accttcacaa aaccggcaat gttcgcgccc tgcacgtagt
    28501 tggtttgctc accttcacca ccatgctcaa cacaggcatg gtggatatcc agcatgatgt
    28561 gatgcaaacg tgcgtcaact ttctcggctt tccagcccag gcgcgcagcg ttttgtgcca
    28621 tttccaggcc cgacgttgct acaccgccag cattagccgc tttacccggt gcaaacagta
    28681 cgcctgcctg ctggaacagt tcagtcgctt cgatggtggt cggcatattt gccccttcag
    28741 cgacggcttt aacgccattg gcgataagct gatgcgcggt gtcaacatcc agttcgttct
    28801 gggtggcgca aggcagggcg atatcaaccg gtacagacca cggctgttgg ccttcgagat
    28861 agaccagacc aaattctttg gcgtaatctg ccactcgacc atcgcggctg gctttgattt
    28921 cgataagacg tgccagtttc tctttcgtga atccgctttc atcaactaca gtgccgctgg
    28981 agtctgacgc agtgatcaca cgagcaccaa attccatcgc tttttcgata gcgtactggg
    29041 cgacgttgcc ggagccagaa acggaaacgc gcatcccttc aaaacccata ccgtggcgtt
    29101 ttaacattgc ttctgtgaaa taaaccagac cgtagccggt agcttccggg cgaataagac
    29161 taccgccaaa tgaaaggccc ttaccggtga agacgcaggc ggtattgttg gagagctttt
    29221 tcatcatccc cgccataaag ccgacttcac gaccaccaac gccgatatca cctgccggaa
    29281 cgtcggtatc cgcgcccagg tggcgataca gttcagtcat cagcgcctgg caaaaacgca
    29341 tcactttacc ttcgcttttt cctttcggat cgaaatcgct gccgccttta ccaccgccca
    29401 tcggcagggt agtcagggca tttttgaagg tttgttcaaa gccgaggaat ttgagaatgg
    29461 aaaggttaac tgacggatgg aagcgcatac cgcctttgta cgggccgatg gcagagctga
    29521 actgcacacg ccatgcacgg ttgacctgta tctggttgcg atcatcaacc cataccacgc
    29581 gaaactggat cacgcgctcc ggttcaacca gacgctccag taatgacatc tggcgatatt
    29641 ttggattttg ttcaagaaaa ggccagagtg tggtcattac ttcacgaacg gcttgcgcga
    29701 actcggtttg attcgggtcg cgcttttgga catggttgag gaatgactcc agagaatatg
    29761 tctgatccat agatataaaa accttatata ttaatacgat tgtgcttatg ttgtttatgt
    29821 catgtgcttt tgcagttttc gactatacca tcaagaatgt gacggtaagc aagaaaaaaa
    29881 tgacccagga aagcaaatta ataacgagag taatctcata accaaatgcg ataactcaga
    29941 cgctaaagtt ttgccacccg gctaccgtta tcagtagcag agacaataac gttaaggagt
    30001 tccctgtgag tcgagcattg ttcgccgtag tattagcatt tccattaatt gcactggcta
    30061 acccgcatta tcgtccggac gtggaggtaa acgtaccgcc agaagtgttc agttcaggcg
    30121 ggcagtcagc gcagccctgt actcagtgct gcgtttatca ggatcaaaat tattctgaag
    30181 gggcggtaat taaagccgaa ggaatactgc tgcaatgcca gcgtgatgac aaaaccctta
    30241 gcactaatcc gctgctctgg cggcgcgtaa agccataaac gcctctaata atggaatgtc
    30301 agcaggggcc agcggatatt gcagcgcctc ttcaggtgag caccagacca gcgcctgatg
    30361 ttcatgtgcc tgtaacgtcc cgtggaagtc gggtacgtgc caggcatgaa gatggataat
    30421 ccggcccgaa acttctcgct gatggctggc aacatactca cccacagttg cttcgatgtc
    30481 caattcttcg tttaactcac gcaccagcgc ctgctgctgg ctttcatcca gctcgacttt
    30541 acctccggca aactcccata atcccgcctg atcgctctgg gcggggcgtt gcgcgagtaa
    30601 aattttgcca tcacgttcaa tgatggcggc aacaacttca atcattttca taaattacac
    30661 acgggcagat aagcgaaaaa tgccataata tccgcactgt tgctcaatca gaaaggcttt
    30721 atatttaagg agtgcctgtg ctagaccgcc atcttcatcc ccggattaaa ccgttgttgc
    30781 atcagtgcgt gcgggttctt gataaatcgg gcattacgcc agatggttta acattagtgg
    30841 gatttgccat cggcgtgctg gctctgccgt ttctggcgct gggctggtat ctggcggcgc
    30901 tagtcgtcat tttgttgaac aggctgcttg atggtctgga cggtgcgctg gcgcggcgca
    30961 gagggcttac cgacgcgggc ggctttcttg atatttctct cgattttctc ttttacgcgc
    31021 tggtgccgtt tggctttatt cttgctgcac cggagcaaaa tgcgctggca ggcggctggt
    31081 tgttgtttgc gtttatcggc acgggcagca gttttctcgc ctttgctgca ctggcagcaa
    31141 aacatcagat tgataacccc ggttatgcac ataagtcgtt ttactatctg gggggattaa
    31201 ccgaaggcac agaaacgatc ttactgtttg tgctgggatg tttattccct gcatggtttg
    31261 cgtggtttgc gtggtttgcg tggtttgcgt ggtttgcgtg gtttgcgtgg atcttcggtg
    31321 cgttgtgctg gatgacgacc tttacgcggg tgtggagtgg gtatctgacg ctgaagtcgc
    31381 tccagcgtca gtaattgcaa tgttatttgc tactgcccgg gccgcgttca ccggttgcaa
    31441 ccggattttt tggatcgctg ctccattcgt accagccgcc gtcatacacg gaaacattct
    31501 tccaacccat ggcgcgtgcg tacataaagg tttcggacgc gcgccagccg gtgccgcagt
    31561 agaatgaaac ttgctgatct ggtttgatat tccatgcttt ccacatagcg gtaatatcat
    31621 cggcgctacg catggtgcca tccgggttat ggaaatcttc catatgcgtc gagtcgctac
    31681 cagcgtgtcc ccaacgtgct ccggctattt caccttttgg tttaatatag ctgtaaccgc
    31741 tggtcgtacc gataaattct ggccacgaac gaatgctcac cagcgatgca tcctggcgat
    31801 gcagcagtcc acgggcctgt tccatatcga gcatcaactg cggttgtgcc gggatcttca
    31861 cgccgaaatc tggttccgct ttcactttcg gtggcgttcc tcgctcaaca ggcagtcccg
    31921 cgtcggacca ggtttgccag ccgccatcca gcagacgcac atctttcacg cctgcgtaga
    31981 gcataatttg cgccacacgc gctccagcgt atacgtcacg cccgtacaga atgaccgtgg
    32041 tgtcatggcg aatgccgtgt tttgccagca tcgcttttag ttgttcatca gaaactttgt
    32101 tccacagcgg ttcgctttcc acttcgttgg tatcgatgta gtcagcgcca ggaatatggc
    32161 tgataaggta aagcttagga gcgccccagg ccgcttcaat gactttccag ccaccgacag
    32221 gtttcgccgt aacctcttta ccttgttgca ggtcatgcag ccattgcgga taaaccagct
    32281 gctcaaagtg cggcagtttt tgtagacggg aaggttcgct tagcgcgtca ctcagaatgg
    32341 agatatgcgt aaaacctgct ttttgcagtc gcgttttgac ggcgtcgaca tctttgccat
    32401 taccgtacag cgccaccggg gcatcggctt tcaggttatg ttgcttgatc cacgcgttga
    32461 gctgttcggt gctcattttg tcgagccagc tagcagagag gtttaaggcg gcaggttcat
    32521 gaccagaagg gccatttaag gtttgtggcc agccgttata aaacgcgctg gggcgagtat
    32581 ctatcgcttt gccattttgt tgctgaagtt ggtcaagtgt aagaggcttc gccagttcag
    32641 cggcccacga agaagcgcaa gccagcccta aagccattgc cagcgcggtc atttgagaaa
    32701 cacgtttcat cgaaaatccc gacattatag ttttgagtgc gccattctga actgacgcat
    32761 tgagaaaaac tttgcgttat cgcagtttgt tgtaattttc tgaccactgc gccatatcca
    32821 gaacagaact atcagcagga acatcctgga gatcgtgcgt tacctgaacg acggggatcg
    32881 ccagggcgcg aacttcgctg aacacccact ggcgaaaatt atcgcgcaga gccacatcaa
    32941 gacggctgaa tggctcatcc aggagtaacg cttttggctg ggcgagaagg gcgcgtagta
    33001 gagcaacgcg cgctcgctga ccgccagaca aagtggcagg atcctgatgg aaagctccgt
    33061 caaggcctga acgctcaagt gcatcattca cggcattacg tcgggcattc cccttaagtg
    33121 tcgccggtag cgccagcagt aaattttgcc cgacgctgaa ctggtcaaat aacagtgcat
    33181 cctgaaaaag aatgccaatc tgacgctgtg cggtgggtag catgtcaatc cgttgctcat
    33241 tgagccatag ctcacctgta caagaaaact gtccggccag tgcaccaatc atccatgaaa
    33301 acagagtgga ttttccacag ccagatggcc ccattaacgt gacgatgtca cctttatcaa
    33361 ccgtaaagtt aacgtttgtc agcaagcggc tttctggtaa acgtagcgaa acatttttca
    33421 cgcagagcat tagcggattc cttgtctgac ataacctacc cattttgcga ctaacgcggt
    33481 cagggcaaaa ataataagcg gtaacaacag ttgccataaa gcctgggcgg cgagaatacc
    33541 gttgctgccg ccgctgctta atgccaccgc ctcagtggtg agcgtcggaa aacgccccgc
    33601 gcccagccac agcgtcggca tatactgcgc aatactgact gaaaatccca ccgcgaaggc
    33661 aatcagcaca gggcgcaaca tgagtgggca tttcacgtaa aagaagattt tggcccgcga
    33721 ccagcccagt gtttgcgcaa tcaaaattaa ccgtgaatca atgcgctgcc aggcgggttg
    33781 caggataaac agcatccacg gcattaccca cagcagatgc ccccagacca ccgccgtcca
    33841 gcttccatcc aggttcagcc atagcgccag cgtgtactag cccgccacca gtggcagagc
    33901 gggcaataga atgggcaacc atagccacaa ctggcgacgc tgtgcccccc attccagcca
    33961 cagcagtaac aggagcaaag cgatgaatgt tgctaccagc cccattgtca gactgttgat
    34021 gagtgcttca ctattgatcg tcgactgatc cgcgaggatc gccagcagaa ccacacacag
    34081 cacaccggtt aagggtaaaa aactcgccag cgtagtgcct ggtaataaag gtgtggcagg
    34141 cttgcgaatg ccatctacgc ggggaatagt gcgccgccag ctgcgccata gcagatagct
    34201 cagcaaaacg taggcggcga gtaacagcat cagcagcaga ctggcgagtg cgcctttcgt
    34261 ttgttgatcc gcgtcgccct gagttaacca ctgccagcta attactgcca gcgtcggtgg
    34321 attacctggc ccaagaataa tcgccacatc cacgaccgaa agcgaccagg caacaatcgc
    34381 cagcatcgcc attgccagcg aaggcgcgac ggagggcaac agcagccagt tcaggcattg
    34441 ccagcggctg tagcccagcg aatccagcac aatgacctgc tgcaacagcc gtttttcgct
    34501 caatactgcc actaagatcc acagcagaaa tgcgctttct ttcactgcaa gggtgaggcc
    34561 cagaccgatc caaatcggtc cattggcgga gtgaaatacg ggaaatagtc atatagcagc
    34621 cctccgtcag caaagagcag cagggcgctg gtggcaaaag ccacatgggg aatggcgagc
    34681 agccacggca gacgggcgca catacgctgc catttcggcc caggccatag cgccacaatc
    34741 accagcaggg caatcaacaa tgccccgacc gccgcgatgg ttgtcgacac cagcgttgcc
    34801 agtaatgcct gcggtaactg tggatcggca aataacgcct gccagcgtgt caacgacaac
    34861 gcaggcgata tcagcgtgag agctgccggg atcagcggtg cataaatcac cgccaccatc
    34921 gcccacagca aaaagattaa tgcgtaccgt aacggcgtaa ccattcttgt tccagtgcat
    34981 tcacccatcc tgcgtgcggt tcagccagta ccggcggcag atcctgcggc attcttgatt
    35041 gcaatatttc gcgctgcccg tcaggcagtt tttgcggatc gagaacagaa ggatcgcccc
    35101 agacaacggg atctgcttta cgcagttgcg catcgggtga gagcaggaaa ttggcaacta
    35161 ccttcgccgc agcactggca ttcgcgttgg ctggaatggt gacgaaatgc acgttgccaa
    35221 tcatcccctc gcgaaagcca aaactgtaac tgcttgccgg caaatcgccg ctggcg
//
LOCUS       DAJTRQ010000025        34877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000025 DAJTRQ010000000
VERSION     DAJTRQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..584
                     /locus_tag="OO332_001368"
     CDS             <1..584
                     /locus_tag="OO332_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5941908.1"
                     /translation="TLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKV
                     TTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQL
                     EYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARN
                     ILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(628..5245)
                     /locus_tag="OO332_001369"
                     /pseudo
     CDS             complement(628..5245)
                     /locus_tag="OO332_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5338..5985)
                     /gene="rnt"
                     /locus_tag="OO332_001370"
     CDS             complement(5338..5985)
                     /gene="rnt"
                     /locus_tag="OO332_001370"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5941909.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(6088..6495)
                     /gene="gloA"
                     /locus_tag="OO332_001371"
     CDS             complement(6088..6495)
                     /gene="gloA"
                     /locus_tag="OO332_001371"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5941910.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6576..7673)
                     /gene="nemA"
                     /locus_tag="OO332_001372"
     CDS             complement(6576..7673)
                     /gene="nemA"
                     /locus_tag="OO332_001372"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5941911.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7710..8309)
                     /gene="nemR"
                     /locus_tag="OO332_001373"
     CDS             complement(7710..8309)
                     /gene="nemR"
                     /locus_tag="OO332_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5941912.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8412..8651
                     /locus_tag="OO332_001374"
     CDS             8412..8651
                     /locus_tag="OO332_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5941913.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8700..9596
                     /locus_tag="OO332_001375"
     CDS             8700..9596
                     /locus_tag="OO332_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5941914.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9677..10006
                     /locus_tag="OO332_001376"
                     /pseudo
     CDS             9677..10006
                     /locus_tag="OO332_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
     gene            complement(9977..10360)
                     /locus_tag="OO332_001377"
                     /pseudo
     CDS             complement(9977..10360)
                     /locus_tag="OO332_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(10353..11144)
                     /locus_tag="OO332_001378"
                     /pseudo
     CDS             complement(10353..11144)
                     /locus_tag="OO332_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(11147..11383)
                     /locus_tag="OO332_001379"
     CDS             complement(11147..11383)
                     /locus_tag="OO332_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5941915.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            11584..12018
                     /gene="slyA"
                     /locus_tag="OO332_001380"
     CDS             11584..12018
                     /gene="slyA"
                     /locus_tag="OO332_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5941916.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(12065..12532)
                     /gene="slyB"
                     /locus_tag="OO332_001381"
     CDS             complement(12065..12532)
                     /gene="slyB"
                     /locus_tag="OO332_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5941917.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            12806..13915
                     /gene="anmK"
                     /locus_tag="OO332_001382"
     CDS             12806..13915
                     /gene="anmK"
                     /locus_tag="OO332_001382"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5941918.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            14013..14342
                     /gene="mliC"
                     /locus_tag="OO332_001383"
     CDS             14013..14342
                     /gene="mliC"
                     /locus_tag="OO332_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5941919.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            14401..15057
                     /gene="pdxH"
                     /locus_tag="OO332_001384"
     CDS             14401..15057
                     /gene="pdxH"
                     /locus_tag="OO332_001384"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5941920.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            15186..16460
                     /gene="tyrS"
                     /locus_tag="OO332_001385"
     CDS             15186..16460
                     /gene="tyrS"
                     /locus_tag="OO332_001385"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5941921.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            16519..17388
                     /gene="pdxY"
                     /locus_tag="OO332_001386"
     CDS             16519..17388
                     /gene="pdxY"
                     /locus_tag="OO332_001386"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5941922.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(17432..18037)
                     /gene="gstA"
                     /locus_tag="OO332_001387"
     CDS             complement(17432..18037)
                     /gene="gstA"
                     /locus_tag="OO332_001387"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5941923.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(18143..19645)
                     /gene="dtpA"
                     /locus_tag="OO332_001388"
     CDS             complement(18143..19645)
                     /gene="dtpA"
                     /locus_tag="OO332_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5941924.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(20256..20891)
                     /gene="nth"
                     /locus_tag="OO332_001389"
     CDS             complement(20256..20891)
                     /gene="nth"
                     /locus_tag="OO332_001389"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5941925.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(20891..21586)
                     /gene="rsxE"
                     /locus_tag="OO332_001390"
     CDS             complement(20891..21586)
                     /gene="rsxE"
                     /locus_tag="OO332_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5941926.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(21590..22210)
                     /gene="rsxG"
                     /locus_tag="OO332_001391"
     CDS             complement(21590..22210)
                     /gene="rsxG"
                     /locus_tag="OO332_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5941927.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(22214..23272)
                     /gene="rsxD"
                     /locus_tag="OO332_001392"
     CDS             complement(22214..23272)
                     /gene="rsxD"
                     /locus_tag="OO332_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5941928.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(23273..25399)
                     /gene="rsxC"
                     /locus_tag="OO332_001393"
     CDS             complement(23273..25399)
                     /gene="rsxC"
                     /locus_tag="OO332_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5941929.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(25392..25970)
                     /gene="rsxB"
                     /locus_tag="OO332_001394"
     CDS             complement(25392..25970)
                     /gene="rsxB"
                     /locus_tag="OO332_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5941930.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(25970..26551)
                     /gene="rsxA"
                     /locus_tag="OO332_001395"
     CDS             complement(25970..26551)
                     /gene="rsxA"
                     /locus_tag="OO332_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5941931.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(26628..27068)
                     /locus_tag="OO332_001396"
     CDS             complement(26628..27068)
                     /locus_tag="OO332_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5941932.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(27154..27369)
                     /gene="ydgT"
                     /locus_tag="OO332_001397"
     CDS             complement(27154..27369)
                     /gene="ydgT"
                     /locus_tag="OO332_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5941933.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(27642..27767)
                     /gene="blr"
                     /locus_tag="OO332_001398"
     CDS             complement(27642..27767)
                     /gene="blr"
                     /locus_tag="OO332_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5941934.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            28010..29050
                     /locus_tag="OO332_001399"
     CDS             28010..29050
                     /locus_tag="OO332_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5941935.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(29086..30087)
                     /gene="add"
                     /locus_tag="OO332_001400"
     CDS             complement(29086..30087)
                     /gene="add"
                     /locus_tag="OO332_001400"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5941936.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(30190..31362)
                     /gene="malY"
                     /locus_tag="OO332_001401"
     CDS             complement(30190..31362)
                     /gene="malY"
                     /locus_tag="OO332_001401"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5941937.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(31372..32964)
                     /gene="malX"
                     /locus_tag="OO332_001402"
     CDS             complement(31372..32964)
                     /gene="malX"
                     /locus_tag="OO332_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5941938.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            33139..34167
                     /gene="malI"
                     /locus_tag="OO332_001403"
     CDS             33139..34167
                     /gene="malI"
                     /locus_tag="OO332_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5941939.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            34279..>34877
                     /gene="hdhA"
                     /locus_tag="OO332_001404"
     CDS             34279..>34877
                     /gene="hdhA"
                     /locus_tag="OO332_001404"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5941940.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSV"
BASE COUNT         8703 a         8944 c         9069 g         8161 t
ORIGIN      
        1 agacgctggc gagacttcag cgacagttaa gccggaaggt caaattcagc aacaactggc
       61 agaagcagaa acgcaaaata cagcgactgc attcctgtat cgcaaatatc cgcagggact
      121 accttcacaa agtcacaacg accgtcagca aaaaccacgc aatgatagtc attgaggatt
      181 tgaaggtcag caacatgtca aagtcagcag cgggtacggt cagccagccg gggcgcaatg
      241 tccgggcaaa atcaggttta aaccgttcga tactggatca gggctggtat gaaatgcgcc
      301 gccagcttga gtacaagcag ctctggagtg gcggtcaggt gcttgctgtt ccgccagcgt
      361 acacaagcca gcgttgcgcg tgctgtggtc atacagcgaa agaaaaccgc ctgtcacaaa
      421 gtaaattcag atgccaggta tgtggatata cagcgaacgc cgatgtaaat ggcgctcgta
      481 acattttagc ggcggggcac gccgttcttg cctgtggaga gatggtgcag tcaggccgct
      541 cgttgaagca ggaacccacc gaaatgattc aggcgacagc ttgaacgtag cagggaacca
      601 cgtctttcag ggcgtggagg atgtcaacta tccccaatcc agcccttgtg gcgaacttga
      661 aaatcccgcc tcacgcagca gcgtaaacat cggcgtttgc cggaccggaa gatcattcac
      721 ttctgttagc gtaaagcgca gccttggttc gcgacgcagt gcggtggtca gcgcctggaa
      781 aatctctggg gcgagtaatt cctccttttc ctgccagacc agcatttttt tgccgccttg
      841 cggcagatag agtaacaatt tgccgccaga aacgaccacc agcgcacccg cccgacgcgt
      901 tggtaccagc gctgccggat gtgcaggcca gggcagaaga tttccccaca cattagccgg
      961 atcgttggcg gagagcgcca ctggtgtata gtggcgtgtt tgcgtggctt gtgtcgccag
     1021 atcgcgcaat cggtcaatag tcagacgttc agcgaattgc gcgccaccca gaccttctac
     1081 aaaacgacct cgcataattc gcccggagtc ttccatactt cgacaaagtg tttgcatcga
     1141 tggaaacccg ccagggatat tttcggctat caccgcctga cgactgatga tgccgtagcg
     1201 gtcgagcata ttttccgcca gcgccagcat cctttcggta tcgtttagtg gctccacctg
     1261 caataacgac cagcgtccag ccagatttgg tgtgttgtaa gatacccgcg gcgagacggg
     1321 ttgtgcatag acaggacgcc cacgacgagc ccggtgactg cggcgagttg aggtgcgtgc
     1381 gttggaactg ctgcgggtga gggcgcgtaa cggtgcccaa atatcgctgg taatgacgcc
     1441 ttgccagacc agcgcccata acgcctcttg cagtgccgag gaatcaaccg attcgccgat
     1501 tttatcgcgt atccgttggc tgatttgttg tgcaaaccag gctcctccgt cagccagaac
     1561 agcgattatc gcttgctgca gcgccgaacg attcgcctga tccgcttcgg cgggagtgaa
     1621 cgattctgcg gcatatttct gtagatgcag tgccaccagg ccgtcatctt cacccagctt
     1681 ttttttgtcc cgaccagata accgctccgg ttgccagtaa ttcatcgagc atttctggcg
     1741 aatagtcgcg tacgcgggca ggcaggatct ggctttccca gagtgaggcg ggtaaaccga
     1801 ctccggcaag ctgttcgatc acccgcatca cgccatctac gccctcataa acgcctggcg
     1861 atgttgaggc aaagagcgcc gggctaccat cggtggcggg taatacgccc tgacgttcca
     1921 gcagcaatcg cgcataggtc gtggctgcaa cgggacgcgt cgcttctctg gcggcttgca
     1981 gcgagcgcaa acgcagacga cgaaatactt catcgctcac ccagatgtct tgttgcagat
     2041 tcatcaccag accctgttca cgcagttgct gaagctgctc ttcgacaatg gcaataccga
     2101 gactaaactc atgagccagt tgttcagccg tgaccagagc atgagcccgg agatagcgca
     2161 gaaagaggtc gcgaagcggg taactgactc tatgtaaata aatctctggc aatgactctg
     2221 gtagtcgtac gccgagggca tcacgcagcc tggcggcatc atccatacag gcaagacgct
     2281 cctgtccgct aatcatcgca gggaagattc gttttactgc aagaagattt tccagatacg
     2341 acgcaacctc ttcactgctg cctgtatgcc gttgcgccag gtcttcaacg gtcattggcc
     2401 ccagttcgcg cagcaggtcg aacaatcctt cttcaccttt cgctcttctg ccaggagcca
     2461 gtcgttgcaa ctcttcttcc acctggcgaa tgacctgcgg gtcgagtaat tcccccggat
     2521 cgacctgtcc gagtagattg cgcagtaact cgctgtccag cgacagtacg gatgcccggc
     2581 gctctgccag cggggcgtcg ctctggtaca taaattccgc gacatagccg aacaataaac
     2641 ttgtggcgaa aggcgagggc gtagtggtcg ttatatcgga tatttgaatt tcgccaccgt
     2701 tcaggcgacg catcaaacgt tccagtgcgg gaagatcata aacatcttgc agacattcgc
     2761 gtagggtttc gagaatgacc ggaaaatccg gatatccctg agcgatttcc agcaactgac
     2821 tggcgcgcag ccgttgttgc caaagcgggg tgcgatggcc cggcgtgcgg cccggcatta
     2881 ataatgcccg cgcggcgcat tcgcgaaaac gggcggcgaa aagtgccgag ctgcctaccg
     2941 cctcgcggac aatttgcagc aacttttctg gttcaaacaa aaaaatcgcg gcgtcgggca
     3001 atttaccatc agtgtcagga atgcgcgcaa caatgccgtc atcactggcg accacagacg
     3061 cgtcagcgcc ccatagggca tgtattcgcc cggcaatcgc cagcgcccag ggttcatgca
     3121 cccgtcttcc atagggagag tgcaaaataa tacgccagtc gccaatttca tcacggcacc
     3181 gttcgaggac cagatggcga ctccccggaa cgatgcccgt cgcgttgcgt tgctcgtcaa
     3241 tcaacccctg aatattggcg ttcgtatttt cttctgccag ccacggggga atagtcccgg
     3301 aaaagaacgc gccatccgcc agtaaatgaa gaaaatcgcc gatcatctcg cctaattcag
     3361 ccggacgtcc gttaccttca ccacgccaga aggggagccg ggcagaacga cccggagcag
     3421 gagtcacaat cacctgatcg cgggtgattt gctggatccg ccatgaggta gcgccgagag
     3481 tgataatgtc gttcacccgc gactcatata ccatctcctc atccagttca cccacccgcc
     3541 gcgaaccggc cttttcttca ccttcgggta ataacacgct atacatgcca cgatccggaa
     3601 tggtgccgcc gctggtaacc gccaacaatt gagcgccagg tcgggcggtc aatatcccgg
     3661 tctccctgtt ccagaccagt ttggggcgaa aagcagaaaa atcgccagag ggatagcgcc
     3721 cggaaagcat atccagcgtg gcgtcaaaaa cacgtcttgg cagatctttc cacggtgcgg
     3781 cacggcgtac gcgggagtac cattcgtcta cctgtaatgc atccatcgcc gcggcggcaa
     3841 cggtttgctg cgcaaggacg tcgagaggat tatgcggtgg tgtcaggttt tccagcctgc
     3901 ctgcgaacat acactctaca atgactgcgg aatcgactaa atcacgccgg gtacggggga
     3961 aaaacagccc tttagataca ccgccaacct gatgtccggc gcgaccaatg cgttgtaacc
     4021 cactggcaac agaaagcggc gttgccacct gaatcaccag atccaccgcg cccatatcaa
     4081 tccccagttc aagactggag gttgcgacca cgcagcgtaa ttcacccgat ttcagcgcct
     4141 gttcggtgat tgctcgttgt tctttagaga cggagccgtg gtgtgagcgg gcaataaaaa
     4201 cgtcgctact ttgtacacgg ttagaggttg cgccggaggt cgactcgaaa tgggccgcat
     4261 caacggcgat agacggggaa cgctgtaagc gtgcggcgta aagctcattt aatcgtgccg
     4321 tcagtttttc cgccagcccg cgtgaattag taaagacaat ggtcgagcga tggcgcaaca
     4381 cttcatcaag gatacccgtt tcaatatatg gccagatgga gccttcccgg ccggcatggc
     4441 tgtcttcgcc ggtgccgctg gcgaccgatg agacatcatc catattggcg accggtacga
     4501 caattcgtat ctgcggatgg cgcattgcgg gcgggttgac taccgtaacc gggcgatcgc
     4561 caccaagaaa tgctgccaca tcgctggctg agcgcacagt ggcagaaagg ccaattcgct
     4621 gtgctgaggt gtggagcagc gcatcgagcc gctccagact taacgccaga tgcgcaccac
     4681 gtttactgcc cgctaccgcg tggacttcat caataattac cgtttcgacg ccgtgtagcg
     4741 tttcgcgcgc gcgggaggtc agcatcagat agagtgattc gggtgtggtg atcagaatat
     4801 ccggcggatt acgggtgagt ttgctgcgtt cctgtgcagg cgtatcgcca gtacggatcc
     4861 ctacgcgaag attgacttcc gtttcgccgc gccgccgccg ttcatcggca atacccttca
     4921 acaggatctg caagttgcgc tgaacgtcgg tgcccagggc ttttatcggt gaaatataga
     4981 ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga
     5041 gccgatcgag ggcgtagaga aatgctgcca gcgttttccc ggagccagtc ggtgcaatca
     5101 ccagcgcatg ttcgcctcgc gccgccacat gccaggtttg cgactggaca gcggtcggct
     5161 gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga aaacacgtcc ggcaggagcg
     5221 atgaagggtc tggattatct gccattaacg ctattccccg tgaatatcat gcttgatgcg
     5281 tggaatagcc tcactataga tgcctgaatc attgcatgtc atcaggcatc gactcgatta
     5341 cacctcttcg gcggcaggta gcggccagcc tcccagacgt ttccagcggt tgacgatttc
     5401 acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc
     5461 gaagtccatg ccagcggtct ggcaagcctt tgacaatacg gtttgtccga gtgccagccc
     5521 ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac gggttacgtt tcagcgaggc
     5581 gcgttctgcg gcggccatca taaagctgtg atcaaaattg gcgttgtgcg ccaccataat
     5641 ggcgcggtta cagccgctcg ctttaatacc tttacgtaca actttaaaaa tttcgtgcag
     5701 cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc
     5761 gagggcttct ggttgcaaat ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt
     5821 gtccggcatc agccagcctt gttcatccat tttcagggtg atggcggcaa tctcaagcag
     5881 cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg atcacaacag gataaaaacc
     5941 acgaaaacgg tcgcacagac cggtaagttg agcgttatcg gacatctgat tctcttaata
     6001 cggataaatt gcagcgcgca ttatgacaaa tattacttgt cgatgcagta aagatgcggg
     6061 cgcgatgagt tcacgcccgg caggagatta gttgcccaga ccgcgaccgg cgtctttctc
     6121 ttcgattaac tcaattttgt aaccgtccgg atcttccaca aacgcgataa ccgtagtacc
     6181 gccttttacc ggacccgcct cacgggtcac gttacccccg ttttgacgga ttttttcgca
     6241 cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca taagcagtgc cgagttcgta
     6301 tttatccacg ccccagttgt aggtcagttc aatcaccgct tcttcggttt ccgggccgta
     6361 gccaacaaac gccagtgagt atttgtattc cgggttttcg ctggtacgca gcagtttcat
     6421 gcccagcact ttggtataaa aatcgatgga gcgttgcaaa tcgccaacgc gcagcatggt
     6481 atgaagaaga cgcatctttt tatcctcaaa atggctaaca gattcaaaat gttgttttag
     6541 tatagcggcg gctttacgcc gctcgcaatg ttggattaca acgtcgggta atcggtatag
     6601 ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg ggttaagctc agctttgagc
     6661 tgcaagcggg cgaccagatc cgggttcgca atccagtcac gaccaaatgc caccgcatca
     6721 attaaccctt tgccgatcag cgtttcagct ttttctactg tgtatgcacc tgcgccgata
     6781 atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg catcagtata cggttcaccc
     6841 cccgcccaat ctggttctga catatgcaga taagcaatgc cgcgtttacc cagttgttca
     6901 atcagataca gtgcatcggc ttcttcattc gggccgttat ctgtgttctg gaaagtaccg
     6961 attggcgaaa cgcgaatgcc aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc
     7021 acttccagta ccaaacgtgc gcgattttcc acgctgccgc cgtactgatc ggtacgatgg
     7081 tttgaagaag gggaaaggaa ctgatgcagc aaataaccgt gagcagagtg gagctctacc
     7141 agatcaaaac cggcttcacg cgcgttagca atggcctgac ggaaatcatt gacgataccc
     7201 ggaatctctt ccagttcaag cgcacgcggc atggatgttt caacgcggat cgcccgacca
     7261 ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc
     7321 tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg tgtgccacag ctgcaaggcc
     7381 atatgaccat tttcagcatg aacgccagcg gtgatttttt tccatgcggc aatttgctcc
     7441 ggactatgga tgccaggcgc acctgcatat ccttttgcct gggcagaaat ttgcgtggct
     7501 tcactaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
     7561 ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
     7621 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
     7681 cccggtcgtc tggtgataaa aaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
     7741 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcagattc
     7801 gcgccaagcc atagtgcgta aagcacttgt gcctgttgta gcggttcgcc acaaaaggtt
     7861 aaacaatggt tctcacggcc attttccaga gcctgcgaga gcagggcgat cacgccgcga
     7921 gcacctttat ccatcgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
     7981 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
     8041 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagccagt
     8101 cgctggtgat atgcggcgta atgacgctca agcatggcaa cgccaaacgc ttctttagag
     8161 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttagtaa ttcgcttagc
     8221 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccaa gagatgttcg
     8281 cgagtatcat gttcggtgtg tttgttcatg cgttggagtg tagtagacca gtcggtctag
     8341 tgcaagcaaa gttgctactt tggacattaa cagcttcaat aatggtaata atcttatctg
     8401 aggagggcga ggtggcggag caattagagt tctttcccgt ccagagcccg tgccggggaa
     8461 tttgccagtc tgacgaacgc gggttttgtc gcggctgttt tcgtagtcgg gatgagcgtt
     8521 ttaactggaa taaaatgagc gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc
     8581 gattaatgcg caaattacgc gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
     8641 cactcttttg aacctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
     8701 tggttcagcg tattactatt gcgccggaag gcccggagtt ttcccgtttt gtgatgggct
     8761 actggcgatt gatggactgg aatatgtccg cccgccagct ggtcagtttt attgaagagc
     8821 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
     8881 aagcggcgtt tggcgaggca ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
     8941 tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa cgtcattggt cattacatca
     9001 ctgaccgcaa tcacatcatt aagagcgccg aacagtcgct aattaatctc gcgaccgatc
     9061 atctggatct gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
     9121 acgcgttcaa acatctgcat cagagcggca aagtgcgtca ttttggcgta tcgaacttta
     9181 cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt tacccttgcc actaatcagg
     9241 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactacaac
     9301 aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg
     9361 attatttcca gccgctgcgt gatgaactgg ctgtggtggc ggaggagtta aacgcgggct
     9421 cgattgaaca ggtggtttac gcctgggtat tacgtttacc gtcgcagccg ctgccaatta
     9481 tcggctcagg taaaattgag cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga
     9541 cccgtcaaca atggtttcgt atccgtaaag ctgcactggg gtacgacgta ccgtaagcac
     9601 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
     9661 aatgaacgga ggtcctatga aacgttttag tctggccatt ctggcgctgg ttgttgcgac
     9721 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggttgg
     9781 gcagtcaatt ggtagcgtca ccattactga aactgataaa ggtctggagt tttcacccga
     9841 tctgaaagca ttaccccccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
     9901 gccagccacc aaagatggca aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc
     9961 acaaaatacc ggtaaaatta accatcggaa aggcgatgat ggcagccgtg tagcccgcca
    10021 gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca ggcctaggta caaaagccaa
    10081 gccacgccga catgctcaat agaaaaaacc acggctcatt gagcgtatgt ccggcaagaa
    10141 gcagtgccgc aatggctccc agtaaactgc cagcgatgcg tccgaggctt ttgctgatga
    10201 caccgccaac ggtgggaaag ctaaccactg cagccgaggt catcgcccaa tagggttcat
    10261 ccagattcaa ataataggca accgtcagcg ccagacacat ggcaatggta ttgcgtaacg
    10321 cataacgcca ttgcgccagc gtggccctga accagcgcaa agcggacggg aacgcgttgg
    10381 gcgagacgaa cccaggggac gttaggtttc acatccggaa taaggcttga gtcactttca
    10441 acgctctgat cataaatcgc ccgaccgata ctggaaacgt gaccctgtaa cgttttgtta
    10501 tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa cttggtttct
    10561 tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa gggcaaacag cggtttcccg
    10621 gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca cccatccact caccggggcg
    10681 cgaatttccg tttgcgccag ttgccattgt gtctgtttca gcgtcgcctg tgcggcatta
    10741 acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc ggcaaaaata
    10801 aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc gaggtcagat
    10861 tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga tctgaaaggg cgttttatcg
    10921 atcgtgagca gaagttcccc cgcgttaacc agttggttgt cttttatatt cagctcaaca
    10981 atacgtcccg acacctgtgg ggtgatagaa acctgttctg cgtgtatttt cccgtcgcgt
    11041 gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac cgcaactatc
    11101 gctacaatga ttgttgagaa atacttaatt gttttcattg acatagttac cacgcaatca
    11161 gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt aacaagggat
    11221 gccagatgtc accggcgtag atccagccac gaagcagacg gtgtacgaca agccagatga
    11281 cgaatccaaa cgcgaatgct ttgaaaaacg gaggaaaata gacggacgca ccgaacacca
    11341 gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag attcctttac
    11401 gaccgaatat attgcgttag attaataaat attctttaag tgcgaaaaat ttacgcgcaa
    11461 tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt tagcaataca
    11521 attgctgcac actattctaa aagccgcata atatcttagc aagctaatta taaggagatg
    11581 aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg gcgtgctctg
    11641 atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac gttacacaat
    11701 atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat tggcatcgag
    11761 cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat ttcgcgtcaa
    11821 acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc agagccactg
    11881 atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaatattaca tggcatctcc
    11941 gcagaggaac tggagcaact gattacgctc atcgcaaaac ttgagcataa tatcattgag
    12001 ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc acacgcaaac
    12061 ttatttagcg cggagaaacg gtcacctgac tgccattgct ggccagtacg acacgttggc
    12121 ccggagagaa acgagtgttg ccttgtttct gtaccaccat gatggtatta ccatcgtctt
    12181 tacgaatttc cagctcgaca ccctgcgttt tgttcattgc actctgtacg ccctgaccag
    12241 ctacgccacc tgcaacagcg cctgctgcag tagccagaga acgcccggtt ccgccaccaa
    12301 cagtattccc caggaaacca ccaagaacag caccgccaat tgcaccgata acgttggaat
    12361 catcaccgcc ctgaatctgt accggacgta cgttaacgat ggtgccatag ctgacattct
    12421 gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg ttattaacac
    12481 aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc actgaaaaat
    12541 ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat atggcatatg
    12601 tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ataatgaaag taggatcaac
    12661 caattataaa caaaacattc atgatgatta ttgatttgaa gataatgttt tactaccaac
    12721 ggtaaaaatc ggtcattccg catggtattc acgaaaagtt tatgtttgag tggtgcgcaa
    12781 acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg ttatgtcagg
    12841 caccagcctt gatggtgttg atgttgtgtt ggcgacaatt gatgaacacc gggtcgcaca
    12901 gctggcaagc ttgagctggc cgatcccggt gtctctgaaa caggctgtac tggatatttg
    12961 ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac tcgggcgact
    13021 ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac ctgcaggcga gagatatagt
    13081 tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg acgggcgtgg caccacacac
    13141 tttacagatt ggcgataaca atcaaattgt ggcacgcacc ggaattacgg ttgtcggtga
    13201 ttttcgccgt cgcgatattg ccttgggagg acaaggcgca ccgctggtac ctgcgttcca
    13261 tcatgccttg ctggctcacc caaccgagcg acgaatggtg ctcaatattg gcggcatcgc
    13321 caatctgtca ctgctcattc ctgggcagcc ggttggcggc tacgataccg gtcctggtaa
    13381 catgctgatg gatgcctgga tctggcgtca ggcaggtaaa ccttacgata aagatgccga
    13441 gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca gcgacccgta
    13501 tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac tttaactacg gctggctgga
    13561 gcgccattta cgccattttc cgggtgttga tccccgtgat gttcaggcca cactggcaga
    13621 actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg aacgattgat
    13681 ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg tattactgct
    13741 aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca tggaagcatt
    13801 ggctttcgcc tggcttgcct ggcggacgct ggcgggatta ccaggaaatc tgccttccgt
    13861 cactggcgca agccaggaga cggtactggg ggctattttc cccgccaact cgtgatatca
    13921 gaattaactg aattttttct tatcgtgtct gccgttacac ttggaagatt aaggagggta
    13981 tttgccctcc ggaccaggaa agtcttcggg atatgactat gaaaaaactg ttaattatca
    14041 ttttgccggt ccttctctcc ggttgtagcg cctttaatca actggttgag cgaatgcaaa
    14101 ccgatacgct ggaataccag tgtgatgaaa aaccgttgac ggtcaaactg aataatccgc
    14161 gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag ggcctttcag
    14221 cctctggcgc gcgttactcc gacgggatct atgttttctg gtcgaaaggt gaggaagcga
    14281 ctgtctataa acgcgaccgc atcgtcttga ataactgtca gttacaaaat ccacagcgtt
    14341 gagatttttc caggggcggc gcacaatagc gtcacccact gacaatccgt aaagaaaacc
    14401 atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata caccaaaggc
    14461 gggttacgcc gccgcgatct tcccgccgat ccattaaccc tttttgaacg ttggctctct
    14521 caggcttgtg aagccaaact ggcggaccct accgcgatgg tggtcgctac cgtggatgaa
    14581 catggtcagc cttatcagcg catcgtttta ctcaaacatt acgacgaaaa aggcatggtg
    14641 ttttacacca acctcggtag ccgtaaagca catcaaattg aaaataatcc gcgcgttagc
    14701 ctgctgttcc cgtggcatac ccttgaacgc caggttatgg tgatcggtaa agcagaacga
    14761 ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag ccagattggt
    14821 gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga aagtaaattc
    14881 ctggagctga agcagaagtt tcaacagggc gaagtgccat tgccgagctt ttggggcggt
    14941 tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg cctgcatgac
    15001 cgctttttgt accagcgtga aaatgatgcg tggaagattg atcgtcttgc accctgaaaa
    15061 gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta ttctatgtct
    15121 ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata tacatggaga
    15181 ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg gtagcccagg
    15241 tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg ctctattgcg
    15301 gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg ttatgcctga
    15361 aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg acgggtctga
    15421 ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa actgttcagg
    15481 agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac tgtggagaaa
    15541 actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg ctgaccttcc
    15601 tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa gcggttaagc
    15661 agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac aacctgttgc
    15721 agggttatga cttcgcctgt ctgaacaaac agtacgatgt ggtgctgcaa attggtggtt
    15781 ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg catcagaatc
    15841 aggtgtttgg cctgaccgtt ccgctgatca ccaaagcaga cggcaccaaa tttggtaaaa
    15901 ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa ttctaccagt
    15961 tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc acctttatga
    16021 gcattgaaga gatcaacgcc ctagaagaag aagataaaaa cagcggtaaa gcaccgcgcg
    16081 cccagtatgt actggcggag caggtgactc gtctggttca cggtgaagaa ggtttacagg
    16141 cggcaaaacg tattaccgaa tgcctgttca gcggttcttt gagtgcgctg agtgaagcgg
    16201 acttcgaaca gctggcgcag gacggcgtac cgatggttga gatggaaaag ggcgcagacc
    16261 tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg cgtaaaacta
    16321 tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa tacttcttta
    16381 aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa aagaattact
    16441 gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat cactccccct
    16501 ggtttttata cagggaacat gatgaaaaat attctcgcta tccagtctca cgttgtttat
    16561 ggtcatgcgg gtaacagtgc ggcagagttt ccgatgcgcc gcctgggcgc gaacgtctgg
    16621 ccgctgaaca ccgttcaatt ttctaatcac acccaatacg gcaaatggac tggctgcgtg
    16681 atgccgccca gccatttaac cgaaattgtg caaggcattg ccattgccgc cattgataaa
    16741 ttacacacct gtgatgccgt attaagtggc tatctgggat cggcggagca gggtgaacat
    16801 atcctcggta tcgtccgtca ggtgaaagca gcgaatccgc aggcgaaata tttttgcgat
    16861 ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat
    16921 gtacggcacg gtttgcctgc cagcgatatc attgcgccga atctggttga gctggaaata
    16981 ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt
    17041 gagcaagggc cacaaattgt gttggttaaa cacctggcgc gagctggcta cagccgtgac
    17101 cgttttgaaa tgctgctggt caccgccgat gaagcctggc atatcagccg tccactggtg
    17161 gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg
    17221 aaactgcttc agggggcaac gctgcaggag gccctggaac atgtgaccgc tgcagtctac
    17281 gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt ggctgctcag
    17341 gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa tatctcattt
    17401 cggcccacaa cgacctgtgg gccgatggct attactttaa gccttccgct gacagcgcgt
    17461 cttgtacttc cggacgttca gccatacgtt gcataaatgc cgcaatgtgc tctaaccctt
    17521 ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag gcatcagcaa
    17581 ttgtaaatct ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca
    17641 gcttcttatc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta tctgggcgaa
    17701 acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt
    17761 tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa
    17821 gatactgcat aatcgcaacg ccttccgtca gcaaagtacc atcgtccagc agcaatgcag
    17881 gcacctgccc cttagggtta acggaaaagt aatcgtcacc gttttcgaga cgttttttca
    17941 ttaaatccac actgacgagc gtaaaatcct ttccgctctc acgcagggta atatgggaag
    18001 cgagagagca ggcgcccggt ttgtagaaca atttcatcgg taactccttt tgtctgtggt
    18061 ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct
    18121 tgtaaaagag tttccctgaa ttttacgcta cagtagcttt cgccgctttg tctgcagcgt
    18181 catcctgcgt catgcggtgc agtttaggcg cggtcagcag catcagtact gcaataacgg
    18241 cagtagcgac accaatctgc aagaatacgc gaccatagac ttccagtgac atcagcgggt
    18301 cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac
    18361 cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt
    18421 gtgcaaccat tgccagaccc agaccagaga tcatcagttc cccgatgctc tgcaggccat
    18481 agcttgcgac cagccagctg acagacacga tacctgcgtc agacgcgaat ttcgcaccca
    18541 gcggcagaat caggaacgca ccagaacaca tcaccatgcc gattgcaaac ttggttggca
    18601 tcggcagggt atcacccatc ttgttataga tagcggccag aatcggacta ccgatgatga
    18661 tccagaacgg gttcagtgcc tgatactgct caggttctac ggccagaccc agaatggagt
    18721 gctcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga
    18781 agaagatgat tgcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac
    18841 ctttcatcgc gaaagcttct ttaccgaaga taaccacaat accgaaggca acaacgccca
    18901 gcgccatacg cgcaacttcc tgattgtgca gtagccaggt ggcaacagcg atcagcgcca
    18961 caacaccaat aatggtcagc aacaggttac ggtagttgac tggttcaaag tctggttttg
    19021 aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc
    19081 ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggggtgg
    19141 ctatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt
    19201 ccagacgtgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa
    19261 acaggccgtt accgaccgca atagccgcca tacccatata aacaataccg gcgtcgtgac
    19321 cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc ataattacgc
    19381 gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca
    19441 gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa
    19501 ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa
    19561 tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag
    19621 ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg
    19681 gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac
    19741 ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat
    19801 ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa
    19861 aaaacgtaac ggcagatttt ttcactgcat tgagaggggg atgatggctt tacggagata
    19921 tgttctgcta tttagcgttt ttcctagtga gatagtccag tttctgaaaa atagccagtg
    19981 taatgttttg caggtcaata tttgttgcgt gtgtaaattt aaatcatttt aacctgattc
    20041 ttgcaatgtt tttacattgc cacagcatcg tgatcttgat cacgaatgaa tagaaaattt
    20101 cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat tacctgatga
    20161 taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg ataaagaaag
    20221 gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt ctctttgtat
    20281 tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat gcaggtataa
    20341 cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg aaccactttc
    20401 agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt acgattacaa
    20461 acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt gtttaatacg
    20521 acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg atcttccgga
    20581 acctcgccat tatgttgttc cagtaatata cggcaggttt tgatgatatt ttctgctttg
    20641 ctgttataaa ggccaatcgt tttgatatag gttttcaccc cttcaacgcc cagttcaagc
    20701 atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt aacactgata
    20761 tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga actgaaatta
    20821 agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc cagacgtttt
    20881 gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt
    20941 cagcacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc atcagtccca
    21001 agccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa
    21061 tctccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg tcaaacaacg
    21121 tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag gttgcgcccg
    21181 taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt
    21241 cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc cctaatgatt
    21301 gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc accgaggcga
    21361 tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa
    21421 tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc agagcgttag
    21481 tggcagtgga cgtgaccgcc aatagcggac aaaggccgag cagttggacc agtgcagagt
    21541 tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt tattctccac
    21601 aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg gcgcgtttta
    21661 ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac
    21721 catctttctt caccgcccag tgcgcatcat ccgcaccact gatttttttt cccgcaaaat
    21781 gggtgagcca gtcagaaagg cgcaattcga ttttatcgcc aagccctggc gtttcgtggt
    21841 gctctgtcac gcgcgtgcca agtaccgtgc cattaaaatc ggctcccacc agcagctgaa
    21901 tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc ggtttgtcat
    21961 cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca gttaccagat
    22021 agcaactctg tgcgagcgca ttgttatagc gttcggctgg cagcacctga ccaaataacg
    22081 ccttttgttg cagactggcc tgttcagcaa ttgtcgtttt ggtcatctgg ttgatggccg
    22141 cagttaaccc tgtagaaccc gctgcaaata gtgccagtgt aatgccgtgt tttcggatag
    22201 ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga
    22261 tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca ggatagccgc
    22321 cgaaactgcg gatcaaccag actaataagc ccgccagcgc gccgaacatc agccgaccac
    22381 gattggtcgt agaagcagta actgggtcag tcaaaataaa gaatgcgccg agcatagtcg
    22441 caccagacag cagatgaatt tgcggtgctg ccagtgtttc tggtgagaac aaccagccca
    22501 acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag cgaatcgctt
    22561 tctgccatag caaccacacg ccaccagcca gccaggcgag atttacccat tgccaaccag
    22621 cgcccgccag aataccgctg tagatcggat attgcataat ctgttcaacc gaatgacctg
    22681 cgcggacaga ggttttgaat gtatccagcg gtgtcgcctg actaatgcca tcaataccta
    22741 agcgtagcgt attcatatca ccaccactgg cggtatgacc gctgaaaata acctgtatgg
    22801 cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct
    22861 gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta aacgggtttt
    22921 gccccagacc gccatacaac tgtttagcga tgatcaccgc aaacaccgta cccagtacga
    22981 ccatccacca tggcgctagg gggggaatac ttaccgccag caataagcct gtcagcaatg
    23041 ctgagttatc tttcaacgtt gcggctaccg actgcttgcg tagtttgagt acgagagctt
    23101 cggctaacag agcactgacc gacgccaaca ggatctgaac gagagtaccc caaccaaaaa
    23161 accacagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaatagc ataatgcgcg
    23221 atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc atttagtcct
    23281 cgtttacaac cttttgctgg gcggcttttt tggcctgaac gcgggcaata gccgcggcaa
    23341 ctgcggcttt gcgcggatcg acctgttctt ctggttccgc attagcctgt tgctgttcca
    23401 gcttgcgtgc tttggcgcgg gcaatagcgg cttcgacggc agctttgcgc ggatcaacct
    23461 gttcttctgg ctccgcattg gcctgttgct gttccagctt gcgtgctttg gcgcgggcaa
    23521 tagcggcttc gacggcggct ttgcgcggat cgacctgttc ttctggttcc gcattagcct
    23581 gttgctgttc cagcttgcgc gctttggcac gggcaatagc ggcttcgacg gcggctttgc
    23641 gcggatcgac ctgttcttct ggttccgcat tagcctgttg ctgttccagc ttgcgcgctt
    23701 tggagcgggc gatagctgct tcaacggcag ttttacgtgg ttcagcaacg gttgctgcgt
    23761 cgttagtttg ctccagttct gcctgtttcg ctctggcttg cgctttacgg gcttcccgtg
    23821 ctgcaataat tgcactgtta tccgggcgtt cgcccgcttt aatcacaata ggctgtgtag
    23881 cctgggcctg tttctctttc acccgcgcca gagcggcagc aatcgcatct ttatctttgg
    23941 ctgcaggttg aacggctgcg ctcttatgtc gttcaaggcg agccgctttt tcgcgctcca
    24001 gacgagcctg gcgcgcttcg aaacgcgctt tggcttctgc ggcgcgcttt tcttcctgac
    24061 gaatagccgc aatttcagct ttttcctgac ggaaatattg caccaaggga atattgctcg
    24121 ggcaaaccca cgcgcaagcc ccacattcaa tgcagtcggc aatgttatgc gtggtagctt
    24181 tatcgtgttg ctgacctttg ctgaaccagt acaactgttg cggcaaaaga tccacagggc
    24241 aggcgtcagc acaggcgcta caccggatgc agctttgttc ttcctgtggt tcgccaagtt
    24301 cattggcaga gggagccaac agacagttgg taatctttac gaccgggaca tccagccatg
    24361 gcaaggtaaa gcccattagc gggccaccca taatcaccat ttgatcggca gaggggcaga
    24421 atccggcatc attcaataaa tgacgcactg gcgttcccag ccgtgcccag acgttgcccg
    24481 ggcgagcgat tgcttcgcca gtcagggtta caacacgctc ggtaatcggc tcgccatcaa
    24541 taacggcacg tttcactgca taagcagtgc cgacgttttg cattaatacg ccgatatcgg
    24601 atgaacgccc gccatgtgga acctgcttcc cggtcagaat gtaggttaat tgtttagcac
    24661 cgccagaagg atatttggtt ggaatcaccc gcagagaaat atcgttagag tccgccagca
    24721 ccgcgcgcag catggaaatc gcctgcggtt tgttatcttc aatgccgata agaatttcgc
    24781 gtggctgcag aatatgcgca agaatgcgaa taccctctac gacctgagcc gcgcaatcct
    24841 gcatcaaacg gtcatcggcg gtaatgtacg gctcgcactc agcagcgttg ataatcaacg
    24901 tttcaatctt atctccacca ccctgcaatt taacgcctgt cgggaatccg gcaccgccca
    24961 gcccggcaac accaaactga tgtatgcgct cgattaactc ttcgcgactg cgagagcgat
    25021 aatcgaccca gccgtcgcgc gggatccagc agtcttcacc atcggcatca ataatcacgc
    25081 ttaattcagc taaagctgaa ggatgagccg tagagtgggg cgcaatagcc gtaacggtac
    25141 ccgaggtggg cgcgtgaaca ggcagcattt tgccgcgacc acgggtaagc ggctggccgc
    25201 gcaatacttt atcgccgacg ctaacgcaca actcaccttc agcgccaata tgctgtttca
    25261 gtggaataac aaaacgctgc gccagtggta cctggcgcag gggggtaccg ttggactggg
    25321 ttttcatctc cggtggatgg atgccgccgt tgaaatccca gattttattt tttctgaatg
    25381 cagagaataa cttaagcatg gtgttccacg ggaatgatac gcacgggaat ggtgttcaga
    25441 tcccatttcc aggagtcagg tgtttctgcg accggttgca acgagatgca gtgcgtcggg
    25501 cacggatcaa cacataaatt gcagcccgta cagagatcac tcattaccgt atgcatggca
    25561 cgggtagcgc caacgatggc gtctaccgga caagcctgaa tacatttagt gcagccaata
    25621 cagttatttt catcaataac cgccaccatc cgcgcaggcg ttagctcttg cgcttcgcca
    25681 tccagcggct gcggctcgac attaagcaac tcggcaattt ttagcatcac agcttcgcca
    25741 cctggggcgc aacggttgat tttttcaccg ttacagctga tggcttccgc gtaggggcga
    25801 cagccgggat aaccgcactg accacactgg ctctgcggta agatttcgtc aattttctca
    25861 acgaccggat cgtcttccac cgcaaaacgg cgggaggcat aacccagaat ggcgccaaac
    25921 gccaggccca gcaggctcac ggcggcaacg gcaatccaga tagcattcat tacaacttca
    25981 ccaaaccact aaagcccata aaggccagag acataagacc tgcggtaatt aacgcaatgg
    26041 cattaccgcg aaaaggtgcc gggacatcag ccacagcaag gcgttcgcgg atggtggcga
    26101 agagcaccat caccagcgag aaaccgacag cggcggaaaa accgtacagc gccgactgca
    26161 agaaattgtg cccgagattg atattcagca acgccacgcc gagcactgca cagttggtgg
    26221 tgataagcgg caaaaaaatc cccagcagac ggtaaagcac cgggctggtt ttgcgcacca
    26281 ccatctcggt gaactgcacg accacagcaa tcaccagaat aaatgccagg gtgcgtaggt
    26341 aaatcagatt aagtgggatc aaaatccacg tatcgataag ccaggcgcaa atagacgtca
    26401 gcgtcatcac aaacgttgtt gccagcccca tgcccatcgc ggtttccagt tttttggaaa
    26461 cccccataaa cggacagaga ccgagaaact tgaccagtac aaagttattg accagtacag
    26521 ttccgacaaa cagtaacagg tagtcagtca tttttcggcc tgaaataaaa aagccgccta
    26581 ttatccgtta atccagagca ggcgacaaca ggttaactgt aaggttatta cggattcaca
    26641 aacgtcgctt ttacccgcga cgagcgtttg aaatagggga cgatcaatgc ggttgccaac
    26701 aaagtaaaca gcaactggcg aacggctatg ccaacttcca cgggtgaaaa agcgaatgct
    26761 tttactgcca gcaatacgga aataagcagc cagataatat agtgtttagg aacacaacgc
    26821 cgacgtttaa agaaagcgat ggtcaaccac agtgtgtaat accacatggc gatagcggta
    26881 ataaaagaaa cgccccataa gatttgtgtc gtcagtgctt gtccgccgag cgtttgaaat
    26941 gtttgcggag aagataacgc agcggtatac agcaacaacg ccagcgtcgt actcaacaga
    27001 gcgactaaaa gccaggccaa cgggccaagc aaccagcctc caatacgttg tggcgttgtg
    27061 gtggtcatag gttctccaga atagcgacgc aaacaggcaa atctaatttt aggggcgtta
    27121 ttataaggct tttataaccg atcgctactt tctttattgg acatagtgcc agacggactt
    27181 cggtacctgg ccgaggtcaa acaaacgccc gccagaaacc agctctgcgc gacggtgatc
    27241 ggcagcacga tacatattga tcagttcctg atcgtccgtc agggtgtaat taagatggtc
    27301 gtagagtttt tccagacttt cgagtgaact gattttgcga aattttaata agtagtcctg
    27361 aacagtcata aaaggtccat aaaataatta ataccaaagg ggtaggtatt attagtgatg
    27421 ataaaatttc accaaaagtg aacgcttgtg ttattacaat aataagaatg cgctgatggc
    27481 aaatattcag ggatgaaaaa ctgctctgaa aagagtaacg cgctttacta tccctgacaa
    27541 tcaccaacaa catcgaacaa gataataaat tcctggttta atatccgaca agtgaaaaca
    27601 tgcacccgga cgggcagcat gtcgctccac aagtgcagag cttacttgtg ttgtaccgaa
    27661 gcactctgtt caggtggctg atagttgtca atgtgactcg ccacgccaag aagaatgact
    27721 gaaacgacaa gaacgatcca acctgttaat tcaataagac gattcattac agcccacatc
    27781 tctcttgatt gatccattaa cttcaggggg taaatgttac ttagcaataa tagctccagc
    27841 aagattttta ctgaggtttt tgcgatattt agcttttgtc gttggaaaat tcgctatttt
    27901 tttgacttaa gttaaacaac atcccttatt gctggcaggt tattaaactg ttgagcgtgg
    27961 gtaaaggata gtgtcaaata gccatcatac ttcaatgaga ggcaatgaca tgagcgacaa
    28021 catccgtgtt gggttgattg ggtatggtta tgcgagcaaa accttccatg cgcccctgat
    28081 tgcgggcacg cccgggctgg aactggcggt aatctccagc agtgatgaaa caaaagtaaa
    28141 agccgactgg ccaacggtta cggttgtctc tgagccgaag catctgttta acgatcccaa
    28201 catagacctg attgtcattc ctacacccaa cgatacccat ttcccgttag ccaaagcggc
    28261 gcttgaggcg ggtaaacatg tggtcgttga taaacccttt accgtgacac tgtcacaagc
    28321 gcgagagctg gatgcgctgg caaaaagcct ggggcgtgtg ctgtctgtat tccataaccg
    28381 tcgttgggat agcgatttct tgacgctaaa aggtttgctc gcggaaggcg tgctgggtga
    28441 agttgcttac tttgagtctc attttgaccg cttccgtccg caggtgcgcg atcgttggcg
    28501 tgaacagggc ggcccaggca gcggtatctg gtacgattta gcaccgcatc ttcttgatca
    28561 ggccattacg ctatttggtt taccggtcag catgacggta gatttggcac agttacggcc
    28621 cggagcgcag tcgaccgatt atttccacgc catcttgtcc tatccacagc ggcgagtcat
    28681 tttacacggt accatgctgg cagccgctga gtcagcacgg tatatcgtgc atggatcccg
    28741 aggcagttat gtgaaatatg gcctcgatcc acaggaagaa cgtctgaaaa atggcgagcg
    28801 tctgccgcag gaagactggg gctacgatat gcgtgatggc gtacttaccc gcgtggaagg
    28861 tgaggaacgt gtcgaagaaa cgctgttgac ggtgcctggg aattatccgg cttactatgc
    28921 ggcgattcgt gatgcgttaa atggcgatgg tgaaaatccg gttccggcaa gccaggcaat
    28981 ccaggtaatg gagttgattg agctgggcat cgaatccgcc aaacatcgcg cgacgttgtg
    29041 ccttgcgtga aaaatggatg gtgcaatttg caccatccat ttttgttact tcgcggcgac
    29101 tttttctcgc agtgcgcgtt tttcctctgc gctgaggaaa gccatttcca gaccattaat
    29161 ctgtgcctgg cggatttgct cacgggataa cccagcagct ggcgcggcaa cggtatattc
    29221 gtgaatgata tccactccct gtacgccggg atcgtcagtg ttaatgctgg cacgaatgcc
    29281 atgctcaagg aacgttttca gcggatgtgc agccagctct gctacggtgc tggtctgaat
    29341 attggaggtc agacaggatt caataccaat ttgttgctcg gcgagaaaat ccatcagcgc
    29401 ccgatcttca atggctttta cgccatgtcc aatacgctcc gcacccagtt cacgaatcgc
    29461 ctgccagatg ctttccggcc cggcagcttc gcctgcatgg acggtaatat gccagcccgc
    29521 atcacgcgcg cggttgaagt gagaaaggaa cagacttccc gggaatccaa gttcatcacc
    29581 agctaaatca agtgcggtaa tctggtcacg gtgggctaaa aaggcctcca gctcttgctg
    29641 acaggcggct tcgccgaagg ttcggctcat aatgccgata agcttcgcct gcacaccaaa
    29701 ggtgcggcaa ccttcacgta cgccatcgat caccgcttcg acaacacccg ctacaggcag
    29761 cttatgtgcc attgccatgt agcctggtga aaaacgcagc tcgacatagc gcaggccgtt
    29821 acgggctgca tcttcaatgt tttcaaatgc cacgcggcgg caggcatcaa gagaggcgag
    29881 aactttaacg ccccagtcaa gtttggtcag aaagctcacc agatcgggtt cgttggcaat
    29941 gacctgaacg tggggaatca gtgtttcaag ggattgtgca ggaagcgaga tattatactg
    30001 gcggccaagt tcaagaatgg tctggggacg aatgttgcca tcaaggtggc gatggatatc
    30061 agttaatggc agggtggtat caatcatggt cgcactcttt ttctggatat aaaagtgcgc
    30121 accattataa aaacaaagca gggtaaaaat ccacgattgc gcaacgattt ttacgttgcg
    30181 caattggggt tagcgaacag cgcggatggc gtttattaat ccagccacac ctttttccag
    30241 tttcgaacgt gggcagccgg cattgagacg gacaaaacca cgaccttctt caccataggt
    30301 ataccccggc atgatcgcga ctttttcttg ctcgataagc gctttttgca acgcgttgtc
    30361 gtcaatattc aacggacgta gatcaagcca ggccagataa gtggattgcg ggatctgcca
    30421 gttgagttca ggaaacgcgg cgttcatttt atctgagata tacgtcaggt tatctttcag
    30481 atagacgcgt aaggcatcca gccacggtgc accttgctga taggcggcga tatgggcagt
    30541 taacgccagt accgaagggg aagaaagccc atcacgtcct ttcagtgccg ataaataggc
    30601 atcacggctg ctgctatttt ctataatccc gtaagcaccg gtcagcgcgg gaatattgaa
    30661 acttttcgat cccgacgtca gcaacgccca gtctccccgc gccacattac tccagggaat
    30721 atgcggctgc tcgccccaga ccatatccat atggatttca tcggaaataa cccgcacatc
    30781 atgacgctcg cacaggtcag ccatgatctc cagctcatcg cacgtccaca ctttcccggt
    30841 aggattctgt gggttacaca ggagcataat tttacattct ggtttcgcca aaacggcttc
    30901 cagcttgccc atatcgcaaa accagccgtc agcctgcttc tctaaagcaa cgggcattac
    30961 tgtgcgctgg ttaccttcaa tggccttgta aaatgcgtca taggcgggcg tgtggatcac
    31021 cacgccttca cctgtttcag accactgacg aatcagttct gaaaccatat agatgacaga
    31081 agggccatac accaccgtct gcgtatcaat tgcggtgtaa tgctgggtgg aaaaccagtg
    31141 ggcaatagcc gcgagaaact catcgttttt ccagcggctg tagccaaata cgccgtgcat
    31201 caggcgctga ttcagcgcct cgataatgca gggggcagtg gcaaaatcca tgtctgaaat
    31261 cgtgaacggt aacaggtcag cagtgccgaa acggtcagcg acataatccc actgtgtaca
    31321 ccatgtgcca tgacgatcca cgacctttga aaaatcgaac atatcttatc cttatgcctg
    31381 gacagtatgc atcagaccgg ccatttcatc ttttactgac tgaacttgtg gcccgataac
    31441 aacctgcagg ttatgttgat taagttgtac tacgccaatt gcccgattgt ccttcagtgc
    31501 ttgcacatta acaagcgaca tatcttccac agacaaacgc agacgggtaa tgcagttatc
    31561 gaggctgaca atattgtcgg caccgcctaa tgcttcgagg attgcaggaa cgttgtaacc
    31621 tgatttaccc ggcgcaccgg caacggcttt ttcgattgag ctggcaactt cgctatcgcg
    31681 ccccggggtt ttcagattga agcgggtgat agcgaaacgg aagatgacgt agtaaacgac
    31741 aaaccagatt gccgccacca ctggcaccat gtaccacttg gttgacagac catgcaaaat
    31801 accgaacacc acgaagtcga tgatattgcc gtcggtatta ccgatggtga cgccgagcac
    31861 agacatgacg gtgaagccga ggccggttaa cagcgcgtgg atgacataca gaactggcgc
    31921 tacgaacagg aacaggaatt ccagcggttc ggtagtgccg ccaacgacgc aggcgatcag
    31981 gccagaaatc agcagacctt taattttatg gcgattttcc gggcgcgcgc agtgatacat
    32041 agctaacgct gcacctggca gaccgccgag aaacgcaggc attttacctt gcgaaaggaa
    32101 acgcgtgacg ctttcagaaa aaccgtgagt ggtcgggcaa ctcaattgcg cctggaagat
    32161 ggtcagtgcg ccgctgacgg tttgaccgca gacctcctgc gtgccgcctg cgtcggtaaa
    32221 gcgaattaat gccaccagaa tgtgatgcag accaaacggt aacagcagac gttcaccggt
    32281 accaaacagc atcggtccga aatcacccgc gctgtttatc atatggccca agccgctaat
    32341 acccatggcg aaaatcggcc agactaatgg aatcaccagg ccgacaaggc ccatcaccag
    32401 cgaggagata attggtacga agcgcgtacc gccgaagaat gccagcgcat ccggcaggcg
    32461 gatattatgg aaacgctcat gcagcatcca gacgataata ccggcgatca ccgcaccgag
    32521 gatcccggta tcgatcgact ggatcccaag aatgctctgg atgttattgg ctttcagaac
    32581 ggcggcatct gtggttggca gaatgccttt attggtcaac cagaagttta ccgcgaggtt
    32641 cattaccgca taaccgacga agccagcgaa tgccgctacg cctttatttt cgcgtgccag
    32701 gcccagcggg atggcgatac agaacatgac aggcaggaaa ctaaaagcaa acgagccaat
    32761 cttactcatc caggtaaaga tagcctgcaa cacggggttg cccaggaccg ggatcagggt
    32821 tatgacatca tggctgctaa gagaactacc aatgccgagc ataatgccgc agaacgacaa
    32881 taatgccacg ggtaacatga aggttttgcc taactgctgg aagaactccc acagcgtgac
    32941 tttcggtgct gttttcgccg tcataaaacg actcctcgta gagaacaatc tggattcagt
    33001 aaattgcgcg agaagataaa acgttttatc aaattttagt gagtcataaa tcacattacg
    33061 caacgataat agcgggtata agataaataa aaggtaaaac gttttatctg tcacataatc
    33121 agggagtagg tcatctgcat ggctaccgcc aaaaaaataa ccattcatga tgttgcgctg
    33181 gctgcgggcg tgtcggtaag taccgtttcg ctggtgctta gtggcaaagg gcgaatctct
    33241 accgccacag gagaacgcgt taacgccgcc attgaagagc tgggatttgt gcgcaatcgc
    33301 caggcgtcgg cgctgcgcgg cgggcaaagc ggcgtcattg gtttgatcgt ccgtgattta
    33361 tctgcgccgt tttacgccga attgacggcc ggattgacgg aagctctgga agcgcaggga
    33421 cggatggttt ttttgcttca cggcggtaaa gacggcgagc agctggcaca gcggttttca
    33481 ctgttactga atcagggtgt cgatggtgtg gtaattgccg gggctgcagg aagcagcgat
    33541 gacctgcggc ggatggcaga agaaaaagct atcccggtga ttttcgcttc ccgtgccagt
    33601 tatcttgatg atgttgatac ggttcgcccg gacaacatgc aggctgcaca gttgttgacg
    33661 gagcatctca ttcgcaatgg gcatcagcgg atcgcctggc tgggagggca aagttcctca
    33721 ttaacccgtg cagaacgggt ggggggctat tgtgcaactc tactaaaatt tggcctgccg
    33781 tttcacagcg attgggtgtt ggagtgcact tccagccaga agcaagccgc ggaagctatc
    33841 acggcgcttt tacgtcataa cccgaccatc agtgccgtgg tttgctataa cgaaactatt
    33901 gcgatggggg catggtttgg tttgctgaaa gcagggcggc aaagcgggga aagcggagtc
    33961 gatcgttact ttgagcaaca ggtttctctg gcggcattta ccgatgcgac accaaccaca
    34021 cttgatgata tacctgttac ctgggccagc acgccagcgc gggaacttgg tatcacactt
    34081 gcggatcgca tgatgcaaaa aatcacccat gaagagacgc attcacgcaa tcttattatt
    34141 cccgcccggc tcattgcggc gaaataatcc tctctttatc tgctatactt ggtagtgtcc
    34201 tttcctcaag gttaatgata aaaaatgtgg cacacaggcg gtgcctttta tttttactga
    34261 agttcacagg aggtttatgt gtttaattct gacaacctga gactcgacgg aaaatgcgcc
    34321 atcatcacag gtgcgggtgc aggtattggt aaagaaatcg ccattacatt cgcgacagct
    34381 ggcgcatctg tggtggtcag tgatattaac gccgacgcag ctaaccatgt tgtagacgaa
    34441 attcaacaac tgggtggtca ggcattttcc tggcgttgtg atattacttc cgaacaggaa
    34501 ctctctgcac tggcagactt tgccgtcagt aaactgggta aagttgatat cctggttaac
    34561 aacgtcggtg gcggtggtcc taaaccgttt gatatgccaa tggcagattt tcgccgtgct
    34621 tatgaactga atgtgttttc ttttttccat ctgtcacaac ttgttgcgcc agaaatggaa
    34681 aaaaatggcg gtggcgttat tctgaccatc acttctatgg cggcagaaaa taaaaatata
    34741 aacatgactt cctatgcatc atctaaagct gcggccagtc atctggtcag aaatatggca
    34801 tttgacctgg gtgaaaaaaa tattcgggtg aatggcattg cgccgggggc aatattaacc
    34861 gatgccctga aatccgt
//
LOCUS       DAJTRQ010000026        34597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000026 DAJTRQ010000000
VERSION     DAJTRQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..1007)
                     /gene="setB"
                     /locus_tag="OO332_001405"
     CDS             complement(<1..1007)
                     /gene="setB"
                     /locus_tag="OO332_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5941941.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PG"
     gene            1375..2505
                     /gene="fruB"
                     /locus_tag="OO332_001406"
     CDS             1375..2505
                     /gene="fruB"
                     /locus_tag="OO332_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5941942.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2505..3443
                     /gene="fruK"
                     /locus_tag="OO332_001407"
     CDS             2505..3443
                     /gene="fruK"
                     /locus_tag="OO332_001407"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5941943.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3460..5151
                     /gene="fruA"
                     /locus_tag="OO332_001408"
     CDS             3460..5151
                     /gene="fruA"
                     /locus_tag="OO332_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5941944.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5575..6516
                     /locus_tag="OO332_001409"
     CDS             5575..6516
                     /locus_tag="OO332_001409"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5941945.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6504..7442
                     /gene="psuG"
                     /locus_tag="OO332_001410"
     CDS             6504..7442
                     /gene="psuG"
                     /locus_tag="OO332_001410"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5941946.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7536..8786
                     /locus_tag="OO332_001411"
     CDS             7536..8786
                     /locus_tag="OO332_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5941947.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8857..9555)
                     /gene="yeiL"
                     /locus_tag="OO332_001412"
     CDS             complement(8857..9555)
                     /gene="yeiL"
                     /locus_tag="OO332_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR5941948.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9686..10627
                     /gene="rihB"
                     /locus_tag="OO332_001413"
     CDS             9686..10627
                     /gene="rihB"
                     /locus_tag="OO332_001413"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5941949.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10727..11977
                     /locus_tag="OO332_001414"
     CDS             10727..11977
                     /locus_tag="OO332_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5941950.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12033..13121)
                     /locus_tag="OO332_001415"
     CDS             complement(12033..13121)
                     /locus_tag="OO332_001415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5941951.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13124..13981)
                     /gene="nfo"
                     /locus_tag="OO332_001416"
     CDS             complement(13124..13981)
                     /gene="nfo"
                     /locus_tag="OO332_001416"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5941952.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14055..15104)
                     /locus_tag="OO332_001417"
     CDS             complement(14055..15104)
                     /locus_tag="OO332_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5941953.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15203..16084
                     /locus_tag="OO332_001418"
     CDS             15203..16084
                     /locus_tag="OO332_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5941954.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16290..17759
                     /gene="lysP"
                     /locus_tag="OO332_001419"
     CDS             16290..17759
                     /gene="lysP"
                     /locus_tag="OO332_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5941955.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18051..20042
                     /gene="cirA"
                     /locus_tag="OO332_001420"
                     /pseudo
     CDS             18051..20042
                     /gene="cirA"
                     /locus_tag="OO332_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20074..20910)
                     /gene="yeiG"
                     /locus_tag="OO332_001421"
     CDS             complement(20074..20910)
                     /gene="yeiG"
                     /locus_tag="OO332_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5941956.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21168..21836
                     /gene="folE"
                     /locus_tag="OO332_001422"
     CDS             21168..21836
                     /gene="folE"
                     /locus_tag="OO332_001422"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5941957.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21853..23010
                     /locus_tag="OO332_001423"
     CDS             21853..23010
                     /locus_tag="OO332_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5941958.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23152..24191
                     /gene="galS"
                     /locus_tag="OO332_001424"
                     /pseudo
     CDS             23152..24191
                     /gene="galS"
                     /locus_tag="OO332_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24471..25469
                     /gene="mglB"
                     /locus_tag="OO332_001425"
     CDS             24471..25469
                     /gene="mglB"
                     /locus_tag="OO332_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5941959.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25530..27050
                     /gene="mglA"
                     /locus_tag="OO332_001426"
     CDS             25530..27050
                     /gene="mglA"
                     /locus_tag="OO332_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5941960.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27066..28076
                     /gene="mglC"
                     /locus_tag="OO332_001427"
     CDS             27066..28076
                     /gene="mglC"
                     /locus_tag="OO332_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5941961.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28147..29382)
                     /gene="preA"
                     /locus_tag="OO332_001428"
     CDS             complement(28147..29382)
                     /gene="preA"
                     /locus_tag="OO332_001428"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR5941962.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29376..30616)
                     /gene="preT"
                     /locus_tag="OO332_001429"
                     /pseudo
     CDS             complement(29376..30616)
                     /gene="preT"
                     /locus_tag="OO332_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30810..31049)
                     /locus_tag="OO332_001430"
     CDS             complement(30810..31049)
                     /locus_tag="OO332_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5941963.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO332_001431"
     CDS             complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO332_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5941964.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO332_001432"
     CDS             complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO332_001432"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5941965.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32935..33630)
                     /locus_tag="OO332_001433"
     CDS             complement(32935..33630)
                     /locus_tag="OO332_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5941966.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33627..34025)
                     /locus_tag="OO332_001434"
     CDS             complement(33627..34025)
                     /locus_tag="OO332_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5941967.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34264..>34597
                     /locus_tag="OO332_001435"
     CDS             34264..>34597
                     /locus_tag="OO332_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR5941968.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGAT"
BASE COUNT         8490 a         8464 c         9103 g         8540 t
ORIGIN      
        1 ccgggcatca aatcctgaaa atagagcatc ccgatgccgc ccagaatgcc aataaaaata
       61 gcatttagca gctgcaagcc caacagaatg gcaggtgaat gcgccatcag cattcctgcg
      121 taaaaacaga cgccacccac ggcagcaacg cgcattaaga aacgcttacc cagacgtttg
      181 gcgaaatatc cggcaatcaa catcgtcggg atttccagcc cggcggcggt ccccatcatc
      241 acaccggcca gtttctcggg aagatgcagt tcgttgataa taaatagcgg catgttgatg
      301 atgtacaggc tgttcgagcc ccacatcaat gtacaaatga caaacagcag tagcgtatcg
      361 cgacggttac ggcgcggcgc ttcaaccgtg ccggtcgcca gcggaagctc tttttgcatc
      421 gacggtaaaa acagccacac catcacgccg caaacaataa acgctaccgc tgcgctcaga
      481 tacattaccg taaagctgaa acccatcgct aacgcataag ccagcggtgg gccaatgacc
      541 catgccagtg aaacctgagc gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt
      601 ttgtcggcat gttcacgggc aagggcaaac atttgcgggt tagcggtcga gccaaagctg
      661 ctaagaaaga cgccaacgaa tagcaaaaca aagtagttgc gattccaggc aaaaagggtg
      721 caggccagca cgcctaacag gcagcaaaag acaatcagcg atttgcgatc gccgcgctta
      781 tcagagcgcc cagcgagaaa ctgacttacc agaatcccaa tgacagcgct gccggtaaag
      841 aagaatccca ccatcgccgg acgggcatgt atttcatcgg taaggaaaat actgagtgtc
      901 ggggtttgca gagcacccgc aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag
      961 gtcaggtcaa acgatttcgc gctggagact gcgggggagt tatgcatgct ggtcgatcac
     1021 tggaagagga agacgcggag tttacgctgc ttgtcagaaa ataaacagac agcgaaatca
     1081 aaagcgccac gaataatcaa aatgccattt cactgttgct gattggctgc tgacgctgct
     1141 gaaatgaaat tgctgataac ggattttccc atcagcaatt aggaaaaatg acaaaaaatt
     1201 gtgcagcaca tcaaactttt gctcataact ttacggcttt ccttgcgtgc tgaaacagaa
     1261 gtattatgct ttcttgaaac gtttcagcgc gatcttgtct ttaaccctaa gccaggttgg
     1321 cgcttttttt ctcatagagg ctgaatcgtt tcaattcagc aagagaggag aactatgttc
     1381 cagttatccg tacaggacat ccatccgggc gaaaaggccg gagacaaaga agaggcgatt
     1441 cgccaggtcg ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc
     1501 atgctggcgc gcgaacagca aacctcaacg ttcctcggca attgtattgc tattccacac
     1561 ggcactaccg acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg
     1621 gaaggcgtca cctggggtga cggtcaggta gcgtacgtgg caatcggtat tgctgccagc
     1681 tcggataaac atctgggcct gctacgccag ctgacccacg tactgagcga tgattccgtt
     1741 gctgaacaac tgaagtcagc aacaacagca gaagaacttc gcgcattact gatgggcgaa
     1801 aagcagagtg agcagctgaa gctcgacaac gaaatgctga cgctggatat cgtcgccagc
     1861 gatctgctga ctcttcaggc gctgaacgct gcgcgtctga aagaggcgag ggcagttgac
     1921 gccactttcg tcaccaaagc catcaatgaa caaccgctga atctcggaca gggtatctgg
     1981 ctgagcgata gcgccgaagg caatctgcgt agtgcgattg cggtaagccg tgcggcaaat
     2041 gcttttgatg tggacggcga aacggcagcc atgctggtga gtgtggcgat gaatgacgat
     2101 cagcccatcg cggttcttaa gcgtctcgct gatttgttgc tcgacaataa agctgaccgc
     2161 ttgctgaaag cggatgcggc aacgttgctg gcgctgctga ccagcgatga tgcgccgacc
     2221 gacgacgtgt taagcgcgga gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca
     2281 ggtaccatgc tggtcaatac cattaaacaa tttaacagtg atattaccgt gacaaacctt
     2341 gatggcaccg gcaaaccggc aaacggacgt agtctgatga aagttgtggc acttggcgtt
     2401 aagaaaggtc atcgcctacg ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa
     2461 gccattggcg acgctatcgc tgctggtctt ggggagggcg cataatgagc agacgtgttg
     2521 ctacgatcac ccttaatccg gcttatgacc ttgttggttt ctgcccggaa attgaacgcg
     2581 gcgaagtgaa cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg
     2641 ccaaagtatt aaaagacctg ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca
     2701 atcaggatgg ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt ttccaggttg
     2761 tacaggggcg cacccgaatt aacgttaagc tgacggaaaa agacggcgaa gtgaccgact
     2821 tcaacttctc gggttttgaa gtcacccccg ccgactggga acgctttgtg actgattctc
     2881 tgagctggct cggtcagttc gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca
     2941 gcccggaagc gttcaccgac tggatgactc gcctgcgtag tcagtgtcct tgcattatct
     3001 ttgatagtag ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac
     3061 ctaaccgccg cgagttggaa atctgggcag gccgtaaact gcctgaaatg aaagatgtga
     3121 ttgaagctgc acatgcgcta cgtgaacaag gtattgcgca tgttgttatt tcactgggtg
     3181 ccgaaggcgc gctttgggtt aatgcctccg gcgaatggat cgctaaacca ccgtcagtcg
     3241 atgtcgtaag caccgttggc gcaggggatt ctatggttgg tggcctgatt tatggcttgc
     3301 tgatgcgtga atccagtgaa cacacactgc gtctggcgac agctgttgca gccctggcgg
     3361 taagtcaaag caatgtgggt attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg
     3421 tcgacttaca accttttaac tgacagcagg agaggcataa tgaaaacgct gctgattatt
     3481 gacgctaatc tcggtcaggc acgcgcctat atggcgaaga ccctgctggg cgcggcggcg
     3541 cgaaaagcaa aactggaaat catcgacaat ccgaacgacg cagaaatggc gattgttctc
     3601 ggtgattcca tcccgaatga cagcgcgctg aacggtaaaa atgtctggct gggtgatatt
     3661 tcccgggcag ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct
     3721 tacactgcgc cggtcactgc gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt
     3781 gcggtgactg cttgcccgac tggcgtagca cacaccttta tggcggctga agccattgaa
     3841 accgaagcga aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg
     3901 ggtaatgcaa tcactcccga ggaagtagcc gcagcggatc tggtgattgt ggcggcagat
     3961 atcgaagtgg atctggcgaa atttgctggt aaaccgatgt atcgtacctc taccggtctg
     4021 gcgctgaaga aaacctcgca ggaactggat aaagcggttg ctgaagcaac gccgtatgaa
     4081 ccggcgggca aagctcaaac ggcgaccact gaaggtaaga aagagagtgc aggcgcttat
     4141 cgtcacttgc taacgggcgt ctcttacatg ctgccgatgg tcgttgctgg tggtctgtgt
     4201 atcgcgcttt cttttgcttt tggtatcgaa gcgtttaaag agccgggtac gttggcagcg
     4261 gcgctgatgc agattggtgg tggttcagcc tttgcgctga tggtgccggt actggcaggt
     4321 tatattgcct tctccattgc cgatcgtccg ggcctcacgc cgggtctgat tggcggtatg
     4381 ctggcggtca gcaccggttc tggcttcatt ggcggtatta ttgcgggctt cctggctggt
     4441 tacattgcga agttaatcag tacgcaactg aaactgccgc agagtatgga ggcgctgaaa
     4501 ccgatcctga tcattccgct aatttccagt ctggtggtcg gtctggcgat gatctacctg
     4561 atcggtaaac cagttgctgg cattctcgaa gggctgactc actggctgca gaccatgggg
     4621 actgcgaatg cggttctgct gggggcgatc ctcggtggca tgatgtgtac tgacatgggc
     4681 ggtccggtaa acaaagcagc gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat
     4741 ggcccgatgg cggcgattat ggcggcaggt atggtgccac cgctggcaat gggcctggca
     4801 acaatggtgg cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa agccgctctg
     4861 gtattggggc tgtgcttcat ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg
     4921 cgtgtgctgc cgtgctgtat cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt
     4981 ggtgcgaaac tgatggcacc gcacggtggt ctgtttgttc tgctgatccc tggcgctatt
     5041 acgccggtat tgggttacct ggtagcaatt attgccggta cgctggtggc gggtttggcc
     5101 tatgccttcc tgaaacgtcc ggaagtggac gcagtagcga aagcagcgta ataaaaggtg
     5161 tgttacaggg cagaaatttc ctgccctgta acatttgatg agcgcaaaga gaatgaccag
     5221 cttgtgaatg taattttatg aatttaaatg gttttatctt tcagtagaaa cttcaaacgt
     5281 tcatcaaaca ctaaggttat ttcctcaagg gcagattatt ttctgccctt ttttagtgtt
     5341 cctgttctgt gtatcccgtc acaaattcct cgcttttgta attcaggcat ttaatcgcat
     5401 tttgtgagcc tgctcaaatc tgcccatttt gcctctctgg cggtaattta ctttacgtaa
     5461 aacatgcata ctatgagcac atgtttaaat atgaacacat gtttaaaccg tctttgcagt
     5521 atctatattc ttgtggattg acagggttgg taatttttta ccagaggacg tgctatgcgc
     5581 gaaaaggatt atgtcgtaat tataggttcg gcgaatattg atgtcgccgg atattcacat
     5641 gaatcattaa attatgtgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagta
     5701 gggcgcaata ttgcacaaaa cctggcgttg ctgggtaaca aagcctggct actgagcgcc
     5761 gtaggcagtg atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggtgtttat
     5821 gtcgataaat gcctgattgt gccgggagaa aatacgtcga gttatttatc attactcgat
     5881 aataccggtg aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct
     5941 gaatatctcg cacagcaccg tgaatttatt cagagggcaa aggtcattgt cgcggactgt
     6001 aatatcagtg aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt
     6061 gtcgatccgg tttcagcgtc gaaatgtgtc aaagtgcgcg accgtctaaa tcagatccac
     6121 actctgaagc caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt
     6181 gacgatgtgg caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg
     6241 agcatgggcg gcgacggcgt ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg
     6301 ccgatcaaaa ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt
     6361 gcttcgtgtt gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt
     6421 tcgtcaatgg cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt
     6481 atatcgttag tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa
     6541 tttctccgga agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcaa
     6601 ccattatttc tcacgggatg ccgttcccac aaaatgccca gatcgcaatt gaagtagaag
     6661 aaactattcg taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga
     6721 aagtgggttt aagcaaagaa aaaattgaat tactgggtcg tgaagggcat aacgtgacca
     6781 aagttagtcg tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg
     6841 tggcttcaac gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa
     6901 ttggtggtgt gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac
     6961 tggcaaatac taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat
     7021 taaccactga gtatttagaa accttcggtg tgccgttaat tggctatcag actaaagcgc
     7081 tgcctgcgtt tttctgccgt accagctcgt ttgacgtcag cattcgtctc gacagcgcca
     7141 gtgaaattgc ccgtgcaatg gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg
     7201 tagcgaaccc gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag
     7261 atcaagccgt cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc
     7321 tgctggcgcg cgttgctgag ctgaccggcg gtgacagcct gaaatccaat atccagctgg
     7381 tgttcaacaa cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt
     7441 aatttctgac gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct
     7501 gacgccgggt ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg
     7561 ggatggtggt gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt
     7621 tgcgcacggt tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact
     7681 tcccaccggg aaaatgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt
     7741 acagtgatgc cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc
     7801 tgtttgacgg tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta
     7861 ctgcgctcat cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg
     7921 gcagcatttt ccagaaagcc ctcaacatca gcaaaatcga atcttttgtt gcggttacca
     7981 ctattttcct cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcgtga
     8041 atcgcaacga gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcgatga
     8101 tgattggtta tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg
     8161 cgatccccgg cggtattttg tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg
     8221 tcacatttga aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg
     8281 cgagcggtgc gatgaccggg ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt
     8341 ttgtcgcaat tattgcgctg atcaacggca ttatcggcgg aattggcggc tggtttggtt
     8401 tcgccaatgc ctctctggaa agtatttttg gctatgtgct ggcaccgctg gcgtggatca
     8461 tgggtgtgga ctggagtgat gccaaccttg ctggtagcct gattgggcag aaactggcga
     8521 ttaacgaatt catcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg
     8581 aagtgaaaac cgtggcgatt atatcctttg cgctttgtgg ttttgctaac tttggttcta
     8641 tcggtgttgt cgttggcgca ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc
     8701 agcttggttt acgggcgctg gcagcagcaa cgctttccaa cctgatgagt gcgactattg
     8761 ccgggttctt tattggtctg gcgtaatgct cgatttgtag accggataaa acgtattaag
     8821 cgttgcatcc ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc cggagaattt
     8881 attctccggg tccatctcca gcgccagtcc tgacaactgc tttctgtttt taattagata
     8941 tcctttcttg cttttaatta ataaaccgtc gtgaatgaat tgcgcgagaa catataaaag
     9001 atgtcgataa gaaacgccta aatactctgc cgcttgcgta tgcttttcgt gataaagatc
     9061 accttcttgt gagagtaata taaatgctgc caggcgattc attaacggaa atgattgatt
     9121 ctgagttaaa gaaacaatat tacgataatt tttatgactt aaggtgacgc aaagttttcg
     9181 taaaaatagg gtgtcgttta ataacagcgg acggtaatgt ttcataggga gcgcaaggca
     9241 ccaacactct tcaatagcct gcaccgcacg cggttcatgg tctttgtcga ttaactcaat
     9301 ctcgccaata aaacagggag cagcaaagaa atcgatcagc gacacgcgac cattagcaag
     9361 cgtggcgtaa agcctggcgc ggcctcgcgt cagataaaac agccatgacg gctgttgccc
     9421 ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc cgcgtatcgg ctgaaacatc
     9481 cgttgaaaag caatccttaa acgcagattc actcatcaat tgctgcttaa ggtcattatt
     9541 gtggatttct ttcacgatgt ttatccgcta tgagatttct catatttagc atacgtacgc
     9601 tgttgttaag attcaatcat cagcccggtc gaattttctt tgctgtagaa aaaaataacg
     9661 caactggaaa cagaggaaat aaacaatgga aaagagaaaa attattctgg attgtgagcc
     9721 gggtcatgat gatgctattg caatgatgat ggcagcgaaa catccggcaa tagatttatt
     9781 aggcatcact attgtagcgg gtaatcagac gcttgataaa acattaatta atggcctgaa
     9841 tgtttgccag aaactggaga ttaatgttcc ggtttatgcg gggatgccgc aacccattat
     9901 gcgtaaacaa atcgttgccg ataatattca cggtgaaacc ggactggatg gcccggtatt
     9961 cgagccgctg acccgccagg cagaaagtac ccatgcggtg aaatatatca tcgataccct
    10021 gatggcaagc gatggcgata tcactctggt gccggttggt ccgctttcaa atatcgcggt
    10081 ggcaatgcgt atgcaacccg caatcctgcc caaaatccgt gaaattgtgc taatgggcgg
    10141 cgcttacggt acaggcaact tcaccccatc tgccgagttc aatatctttg ccgacccgga
    10201 agccgcccgc gtggtgttca cctccggcgt tccattagtg atgatgggcc tcgatcttac
    10261 caaccagacc gtttgcaccc cggatgtaat tgctcggatg gaaagggtag gcggccctgc
    10321 cggagagctg ttcagcgaca tcatgaactt cactctcaaa acgcagttcg aatactacgg
    10381 cctggctggc ggcccggtgc atgacgccac ctgcattggt tatctgatca accctgatgg
    10441 cattaaaacc caggatatgt acgtcgaagt ggacgtcaac agcggccctt gctacgggcg
    10501 tacggtctgc gacgaactgg gcgttcttgg caagcccgct aataccaaag tcggcatcac
    10561 tattgatacc gactggttct ggggcttagt cgaagagtgc gtgcgcggct acatcaaaac
    10621 ccattaagtg ttgataacaa gccgggacag tatcgcaaga tgcaggcccg gctatttctg
    10681 atgggcagaa agccgtcgcc caaaatgaca tgaagagata atcactatgg atgtcatgag
    10741 aagtgttctg ggaatggtgg tactgctggc aattgcgttt ttactgtcag taaacaagaa
    10801 gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta caggtcgtga ttggcgggat
    10861 tatgctttgg ttaccgccag ggcgttgggt cgcagaaaaa gtcgcttttg gcgtccacaa
    10921 agtgatggcg tacagcgacg caggtagcgc atttatcttc ggttctctgg tcggaccgaa
    10981 aatggatacc ttatttgatg gcgcaggatt tatctttggt ttcagggtat taccggcaat
    11041 tatcttcgtc actgcactgg tgagtattct ctactacatc ggtgtgatgg ggatcttaat
    11101 tcgcattctc ggcggtatct tccagaaagc tttaaatatc agcaagatcg agtcattcgt
    11161 cgcggtcacc accattttcc tcgtgctaaa cgaaattccg gcgatcgtca aaccctttat
    11221 cgatcgtctg aatcgcaatg aattatttac agcgatttgt agtggcatgg cctcgattgc
    11281 tggttcgaca atgattggtt acgccgcact gggcgtacct gtggaatatt tgctggcggc
    11341 atcgttaatg gcgatccctg gcgggatctt gtttgcccgc ctgttaagcc cggctacgga
    11401 atcttcgcag gtttctttta ataacctctc tttcaccgaa acaccgccaa aaagcattat
    11461 tgaagccgat gcgacagggg caatgaccgg gctgaaaatc gccgccggtg tagcgacagt
    11521 tgttatggca tttgtcgcca tcattgcgtt aattaacggt attatcggcg gcgttggcgg
    11581 ctggtttggt tttgaacatg cctcgctgga gtccatttta ggttacctgc tggccccact
    11641 tgcgtgggta atgggtgttg actggagtga tgcaaatctt gccgggagtt tgattggaca
    11701 gaaactggca ataaatgaat ttgtcgctta tctcaatttc tcaccctatc tgcaaacggc
    11761 tggcactctg gatgctaaaa ccgtggcgat tatttccttc gcgttgtgcg gtttcgctaa
    11821 ctttggttct atcggggtgg tggtgggggc gttttctgcg gttgcgccac accgtgcgcc
    11881 ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg acactttcta atctgatgag
    11941 tgccaccatt gccgggttct ttattggttt agcgtagtgt gcaatttatg agccggatta
    12001 gatgcggtca agcgtcgaat ccggcaacat tgttacacca ttggcactaa tgacagcgcg
    12061 ttatcggcag acagggtagg gttgtttaag ctgccgctgg cgcgtgaaat tgccgcgcag
    12121 gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct ccagaaagct atataccaga
    12181 cccgccataa aaccatcgtc agcaccaaaa ctgtctaccg tcgtatgcgc tggcacagtc
    12241 agcaaaaatt gttcaccatc cttttcgctg caataaactg actcatcggg caaataaaca
    12301 aacagttgct gaacaccttg ctgatgcaat gcattcactg cggcattacg gtcagcatcg
    12361 ctggtgatcg cctgtcccca taaaatttcc agctccggta aagtgggttt cagggtgtga
    12421 atatgcgcca gccagtgttt gattttgccc gctttgaatt ctgaaacggt atcgacaaac
    12481 accgggatct catcagcgag ggtaaagacc cattccagcg cctcggctgt caggttgcaa
    12541 tcaaccagta ccacgcccgc atgacgaagt aaatcgcgcg acccgttcag tagttgcggt
    12601 gtcaactgtt ccagcagatg ggtatcgtta atcgccagca cggtttgatc gtctcgattg
    12661 gcaattgcca gatacgtcga tgtgctttga ccatgcaaac gaacgcagcc ggagacattc
    12721 acgccggcac ggcgcgtttc ttccaggagc atttcgccat aaaagtcatc gccaatcact
    12781 gaaagcaaat ggacgtcacg gcctaacagc gccagattgt gggcgatatt gcgtccaacg
    12841 ccgcctgccg aacaatgaat tgtaccggga tgagaagccg cttgcgggta atggatatcc
    12901 gccatcccgc gaatatccat attgattgtc cccaccacta cgcagtattc ctgctcggtg
    12961 agaatgtaac ctttgccttt aatccggcct ttgcgcatta aatccataat atgcgccgca
    13021 acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt tctgctgaat cagcgggtta
    13081 cgccgtaaaa ttgcaaggat ctccttttcc cggttattca tcttcaggct accgcttttt
    13141 cagtttgttg cgctttcagc caggcgatct cttctgccca gatatccggg ttgatggttt
    13201 caaggatcag cggaatgccg tcgaaacggt cgttctgcat aatccagcgg aacgcatcat
    13261 gaccgatatt gccttcaccg aggctatgat ggcggtcaac gcggctgcca aaggtacttt
    13321 tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc gacaatacgg gcaaaatcag
    13381 cgaacgtttt ttcgcattcg gctggagtac gcaaatcata cccggcagcg aaagcatggc
    13441 aggtatcaat gcagacgccg acgcgggatt tatcttccac gccgtcgata atcgccgcga
    13501 gatgttcgaa tttaaaccct aagttactgc cctgaccggc ggtgttttca atcaccgctg
    13561 ttacaccttg agttttatcc agcgcaatgt tgatggattc ggcaatacgc gcaaggcaat
    13621 cctcttctga aatctgcatc agatggctgc cagggtggaa gttgagcaaa gaaagcccca
    13681 gttgttcgca acgctgcatt tcatctataa aggcatcgcg cgatttttcc agagcttcag
    13741 tgaccggatg tccgaggttg atcagataac tgtcgtgggg aagaatttgc gccgatgtgt
    13801 agtgatattt ttcacaggcg gctttgaatt catcgatggt ttgcgtcgtg agcggtgcgg
    13861 cacgccactg acgttggttt ttggtgaaca aggcaaacgc ggttgcgtcg atttcggcgg
    13921 cgcgaattgc ggcatttgcc agaccgccag cagcactaac gtgcgctcca atgtatttca
    13981 tgcgaggact cctgttaaac ccgctggagg aaaacggtaa tgatagcggg ttaacaggag
    14041 caagaagtag tggtttatgc gatgacgctt tgaatcacat agttaatcgc accaccacca
    14101 acaatcagcc aggcaaacag taccagtgcc atcagcagag gtttcgcccc agcttttttc
    14161 agcgcgctga cgtgagtggt cagacccagc gccgccatcg ccattgccag caggaaggta
    14221 tccagcgtta ccagcatgtt caccacgctc tgcggtaaca ggtggaacga gttaaagatg
    14281 gcaactacga tgaacaagat ggcaaaccac ggaatagtga ttttgctttt ctcgccgctg
    14341 ttcgccccag acagctgttt aacacgcgcc gccagcagga tgaggaacgg agccagcatc
    14401 atcacgcgca gcattttgga aataactgct gcgttttctg catccgggct gatggcatga
    14461 cctgccgcca ccacctgcgc cacttcgtgc acagtagaac caatgtagat accgaaagtt
    14521 tccggactaa accattgaga catcagcgga tatatcgccg ggtagaggaa aatcgcgacg
    14581 gtcccgaaga taacaacggt tgcaacagcc acggttactt tactggcttc cgctttcact
    14641 accggctcag tcgccagtac cgcggcagca ccacagatac tgctaccggc accgatcaac
    14701 caactggtgt gcttatccag accaaacact ttctgcccca ggaagcaagc cagcaggaag
    14761 gtactggaca gcgtcagcac gtcaatgatg atcccactga taccgacatc ggcaatttgc
    14821 gagaacgtca gacggaagcc ataaagaatg atacccagac gtaataaata ttgcttggca
    14881 aacagcacac caccgtcaca gcttttccag atgtgcggat agatggtgtt gcctaaaacc
    14941 atccccaaca agattgcgag ggtgagggca ctaaacccgg cacccgcaac cgcgggaatg
    15001 gaaccacccc acagggcgac cccggtgata actgcactca gggctaaccc cggaataaaa
    15061 tgccacagtg tacgatgttg tttctgtaag gtgatattcg tcataaccct ctcctttaac
    15121 cgggcataag gttacgacta actggtttaa aaataaaatt gattatatat ttataattaa
    15181 tctttataag tggtaagcga ctatgcacat caccctccgg cagttggaag tttttgcaga
    15241 agtattgaaa agtggatcaa ccacccaggc gtcggtgatg ctggcgttgt cgcaatcagc
    15301 agtgagcgca gccttgactg acctggaagg gcagcttggc gttcaactgt ttgatcgcgt
    15361 ggggaaaaga ctggttgtta atgaacacgg gcggctgctc tatccgcgag cgttggcact
    15421 gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa gacaacggcg cgattcgtat
    15481 ctatgccagt agtaccatcg gtaactacat tctgcctgca gttatcgccc gttatcgcca
    15541 tgattatccg cagttgccga ttgaacttag cgttgggaac agccaggacg tgatgcaagc
    15601 ggtgctggat ttccgcgttg atattggctt tattgaagga ccgtgccaca gcactgaaat
    15661 catttctgaa ccgtggctgg aagacgagct ggtggttttc gccgcgccga cttcgccgtt
    15721 ggctcgtggt ccggtcacct tagaacagct ggccgctgcg ccgtggatcc tgcgtgaacg
    15781 cggttccggc acgcgggaga ttgtcgatta tctgttgctg tcacatttac cgaagtttga
    15841 gatggcgatg gaattaggta actccgaggc aatcaaacat gcggtgcgtc atgggttggg
    15901 aattagttgc ctgtcgcgac gtgtgattga agatcaattg caggcaggca cattaagtga
    15961 agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg cgtatacatc atcggcaaaa
    16021 acacctttcc aacgcgctac ggcgctttct ggactattgc gatcccgcaa atgtgccgcg
    16081 ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt tcgtgacgca gcgccttcag
    16141 catgcattcg ccagaaaaga gattggctgc tttacttata atccctgggc gatcatgaag
    16201 gtgtcttata accgtgtatt tctgccggaa ggattgccaa tcgtctgcta caatcgcgcc
    16261 atcattttta agatggatag catttttgta tgggttccga aactaaaact acagaagcgc
    16321 cgggcttacg ccgtgaatta aaggcgcgtc acctgacgat gattgccatt ggcggttcca
    16381 tcggtacagg tctttttgtt gcctctggcg caacgatttc tcaggcaggt ccgggcgggg
    16441 cattgctctc gtatatgctg attggcctga tggtttactt cctgatgacc agtctcggtg
    16501 aattggctgc atatatgccg gtttccggtt cgtttgccac ttacggtcag aactatgttg
    16561 aagaaggctt tggcttcgcg ctgggctgga actactggta caactgggcg gtgactattg
    16621 ccgttgacct ggttgcagca cagctggtca tgagctggtg gttcccggat acaccgggct
    16681 ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt gaactacatc tctgttcgcg
    16741 gctttggtga agcggaatac tggttctcac tgatcaaagt cacgacagtt attgtcttta
    16801 tcatcgttgg cgtgctgatg attatcggta tcttcaaagg cgcgcagcct gcgggctgga
    16861 gcaactggac aatcggcgaa gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag
    16921 ctatgattgt cggcttctct ttccagggaa ccgagctgat cggtattgct gcaggcgagt
    16981 ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca ggtattctgg cgaatcctgt
    17041 tgttctatgt gttagcgatc ctgattatca gcctgattat tccgtatacc gatccgagcc
    17101 tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt caccctggtg ttccagcacg
    17161 cgggtctgct ctctgcggcg gcggtgatga acgcagttat tctgacggcg gtgctgtcag
    17221 caggtaactc cggtatgtat gcgtctactc gtatgctgta caccctggcg tgtgacggta
    17281 aagcgccgcg cattttcgct aaattgtcgc gtggtggcgt gccgcgtaat gcgctgtatg
    17341 cgacgacggt gattgccggt ttgtgcttcc tgacctccat gtttggcaac cagacggtat
    17401 acctgtggct gctgaacacc tccgggatga cgggttttat cgcctggctg gggattgcca
    17461 ttagccacta tcgcttccgt cgcggttacg tattgcaggg acacgacatt aacgatctgc
    17521 cgtaccgttc aggtttcttc ccactggggc cgatcttcgc attcattctg tgtctgatta
    17581 tcactttggg ccagaactac gaagcgttcc tgaaagatac cattgactgg ggcggcgtag
    17641 cggcaacgta tattggtatc ccgctgttcc tgattatttg gttcggctac aagctgatta
    17701 aaggaactca cttcgtacgc tacagcgaaa tgaagttccc gcagaacgat aagaaataag
    17761 ttttctccct tccttgctaa gccctctcaa ccgagagggc tttttcaatt ccatttccct
    17821 gacaaatcat gcggatataa aatttaacat ttggattgat aattgttatc gtttgcatta
    17881 tcgttacgac gcaatcaaaa aaggctgaca aatcagaggc tgttccggct ttctgggatg
    17941 atcacctgca tacaaataag tccaccgcga tgctgccgta cgcaagggga cgtaaagaag
    18001 atgtgagcga tacccatttt attttcgtag ttacctcatg gagatatgga atgtttaggt
    18061 tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat ttcttgtgca tggcctgtgt
    18121 tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc atcttccgtg gaacaaaatc
    18181 ttaaagatgc acccgccagt atcagcgtca ttacccagga agacctgcag cgaaaaccgg
    18241 tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt acaactgacg aacgaagggg
    18301 ataaccgtaa gggcgttagt attcgtggtc tggacagcag ctacaccctg attcttgtcg
    18361 acggtaaacg cgttaactcc cgcaatgccg tcttccgcca caatgatttc gatctgaact
    18421 ggatcccggt cgattccatc gaacgtattg aagtggttcg tggcccgatg tcgtcgctat
    18481 acggctccga tgcgctcggc ggtgtagtga atatcatcac caaaaaaatc ggtcagaaat
    18541 ggtcgggtac cgttaccgtc gataccacca ttcaggaaca tcgcgatcgc ggtgatacct
    18601 ataacggtca gttctttacc agtggaccat taattgatgg tgtgctggga atgaaagctt
    18661 acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa ctcaacgacc accgataccg
    18721 gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg caatgtcgaa tttgcctgga
    18781 caccgaatca aaatcacgat tttactgccg gatacggttt cgaccgtcag gatcgtgatt
    18841 ccgactagct ggacaaaaac cgcctggaac gccagaacta ctgcgtcagc cataatgggc
    18901 gttgggatta cggcaccagc gaactgaaat cctacggtga gaaagtcgag aacaaaaacc
    18961 ctggcaacag cagcccgata acttccgaaa gcaatacggt cgacggcaaa tacacgttgc
    19021 cgctgacggc gattaatcag tttctcacgg ttggcggtga atggcgtcac gacaaactta
    19081 gcgatgcggt gaacctgacc gggggaacca gctccaaaac gtctgccagc cagtacgcgc
    19141 tgtttgtgga agatgaatgg cggatcttcg agccgctggc gctgacgacc ggcgtgcgta
    19201 tggacgatca cgaaacctac ggtgaacact ggagtccgcg tgcctacctg gtttataacg
    19261 ccaccgacac cgtaacggtg aaagggggct gggcgacggc atttaaagca ccttctctgt
    19321 tgcaacttag ccctgactgg acgagcaatt cctgccgtgg cgcatgtaag attgtgggta
    19381 gcccggatct gaaaccagaa accagcgaaa gttgggagct ggggctttac tacatgggtg
    19441 aagaaggctg gctggaaggg ggtgaatcca gcgttatcgt tttccgtaac gatgtgaaag
    19501 atcgtatcag catcagccgt acgtctgacg tcaacgctgc accgggctac caaaactttg
    19561 ttggttttga gacgggcgct aacggacggc gcataccggt atttagctac tacagcgtta
    19621 acaaagctcg tattcagggc gtggaaaccg aactgaaaat tccgttcaac gatgaatgga
    19681 aactgtcgat caactacacc tacaacgatg gtcgtgatgt cagcaacggc gaaaacaaac
    19741 cgctatccga tctgccgttc catactgcta acggtacgct ggactggaaa ccgctggcgc
    19801 tggaagactg gtcattctat gtttctggtc actataccgg gcagaaacgc gccgacagcg
    19861 cgacggctaa aacaccgggc ggttatacca tctggaatac cggcgcggcc tggcaggtga
    19921 ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg cgacaaggat ctcagtcgtg
    19981 acgactacag ctataacgaa gacggacgtc gttactttat ggcagtggat tatcgcttct
    20041 gatgagaaga tgcccggcga accgggcgga ctttcacttc agtaaatact gcgcatggaa
    20101 gcgcaggtga tcctctataa aagaggcgat gaagtagtaa ctgtgatcat atcccggctg
    20161 aatacgcagc gtcatcggcc acgctttctg gcgcgcggct tctgccaata ccgcaggttg
    20221 caactggtcg gcaagaaact gatcattatc gccctgatcg ataagcgtcg ggatcgcatc
    20281 ctgcgcgtta ctggcataca tcagcgcgca actatcccat tccagccatg catttttgtc
    20341 ctcacctaaa taggtgctaa acgctttgat tccccacggg acgctgcacg gattcacaat
    20401 gggcgcaaag gccgaaacgc tggtgtattt acccggattt ttcagcgcca taatcagcgc
    20461 accgtgacca cccattgagt gaccgctaat ggcgcagcgg tcgctgacat taaattgcga
    20521 ctgaaccagc gccggtaatt catcgcgcag ataatcatac atccggtaat gcgtcgccca
    20581 cggcggttgc gtggcattaa gataaaagcc tgcgccctgg cctaaatcgt agccatcgtc
    20641 gttggcaacc ttttcgccgc gcgggctggt gtctggcatc accagtacaa tccccagttc
    20701 cgccgctacc cgctgggcac ccgccttggt ggtgaagttc tcgtcattgc aggttaatcc
    20761 ggaaagccag tacagcactg gtggcggggt gtgatcacga gggggtggga gaaagatact
    20821 gaacgtcatc gggcagttta aggtactgga gtcgtgtcgc cagcgttgct gccagccttc
    20881 aaaacagcgg tgctcttcga gcatttccat gcgtggctcc ttgttgtgtt gtttgcaatt
    20941 tcttcatcat accgtttatt caatgtgctg tgagtaactt tcacttccgt atttgcataa
    21001 cgatgtttta acatctgctg atgaaaggca gcggcaatta caataattat cgctgtgaat
    21061 actggattat gtgcgccgcc tcacgcacaa taatcaagct gtaaatcagc ttaataactt
    21121 tgcccccacg cagggcggag gcgtcacacc tgcaggagaa atcataaatg ccatcactca
    21181 gtaaagaagc ggccctggtt catgaagcgt tagttgcgag aggactggaa acaccgctgc
    21241 gcccgcccgt gcatgaaatg gataacgaaa cgcgcaaaag ccttattgct ggtcatatga
    21301 ccgaaatcat gcagctgctg aatctcgacc tggctgatga cagtttgatg gaaacgccgc
    21361 atcgcatcgc taaaatgtat gtcgatgaaa ttttctccgg tctggattac gccaatttcc
    21421 cgaaaatcac cctcattgaa aacaaaatga aggtcgatga aatggtcacc gtgcgcgata
    21481 tcactctgac cagtacctgt gaacaccatt ttgttaccat cgatggcaaa gcgacggtgg
    21541 cttatatccc gaaagattcg gtgatcggtc tgtcaaaaat taaccgtatc gtgcagttct
    21601 ttgcccagcg tccgcaggtg caggaacgtc tgacgcagca aattcttatt gcgctacaaa
    21661 cgctgctggg caccaataac gtggctgtct cgatcgacgc ggtgcattac tgcgtgaagg
    21721 cgcgtggcat ccgcgatgca accagtgcca cgacaacgac ctctcttggt ggattgttca
    21781 aatccagtca gaatacgcgc cacgagtttc tgcgcgctgt gcgccatcac aactgattaa
    21841 aaggcaggaa ccatggagcg caacgtcacg ctcgattttg ttcgcggcgt cgccattctg
    21901 gggatcctgc tattaaacat cagcgccttt gggctaccaa aggcggctta tctcaatccc
    21961 gcctggtacg atgctattac gccgcaggat gcatggacct gggcatttct cgatctcatc
    22021 ggccaggtga aattcctcac gctttttgcg ctgctgtttg gtgctggcct gcaaatgttg
    22081 ctgccccgtg gcagacgctg gatccagtcg cggttaacgc tgttagtctt gttgggcttt
    22141 attcacggtt tattgttctg ggacggcgat attctgctgg cttacgggct ggtgggctta
    22201 atctgctggt ggctggtgcg cgatgcgcca tcggtaaaaa gcctgtttaa taccggcgtc
    22261 atgctttatc tggtggggct tggcgttttg ctgttattgg ggctgatttc cgacagccag
    22321 accagccgcg cctggacgcc ggatgcatcg gctattttgt atgaaaaata ctggaagctt
    22381 cacggcggcg ttgaagcgat cagtaatcgt gccgatggtg ttggcaatag tttactggca
    22441 ctgggcgcac agtatggctg gcaactggca gggatgatgc tcattggtgc ggcgctgatg
    22501 cgcagcggct ggctgaaagg gcagttcagc ttacgtcact atcgtcgtac tggttttgtg
    22561 ctggtggcga ttggggtgac cattaacctt cctgccatcg ccctgcaatg gcagctggac
    22621 tgggcgtatc gctggtgtgc cttcttactt caaatgccgc gggaactgag tgcgccgttt
    22681 caggcgattg gctatgcgtc gctgttttat ggcttctggc cgcaattgag ccgctttaag
    22741 ctggtgcttg cgatcgcctg cgtcggacgg atggcgctga ccaactatct attgcaaacg
    22801 ctgatttgta ccacgctttt ttaccacctc ggtttgttta tgcattttga ccgcctggag
    22861 ctgctggcgt ttgttattcc ggtatggctg gcgaatatcc tcttctctgt tatctggctg
    22921 cgttacttcc gccaggggcc ggtggaatgg ctctggcgtc agttaacttt gcgtgctgcc
    22981 ggactggcaa tatctaaaac atcaagataa cgatctggat cacattcgtt aacaaaacgg
    23041 ctgtaaccgt ttccattgct gtgactcgat tcacgaagtc ctgtattcag tgctgacaaa
    23101 atagccgcca gcaagcagtc atttactgca atctcataac aggtagtgaa tatgatcacc
    23161 attcgtgatg tagcgcgtca ggctggcgtc tctgtggcaa cggtttcccg ggtgctcaat
    23221 aacagcacgc tggtcagtgc cgacacgcgt gaagcagtaa tgaaagccgt gagtgagctg
    23281 gattatcggc caaacgccaa tgcccaggcg ctggcaactc aggttagcga caccattggc
    23341 gtggtggtga tggacgtttc tgatgcgttt ttcggcgcgc tggtaaaagc ggtggatctg
    23401 gtcgcccagc agcatcagaa atacgtgcta atcggcaata gctatcatga agcggaaaaa
    23461 gagcgtcacg ccattgaggt gttaattcgc cagcgttgta atgcgttgat tgttcactca
    23521 aaagcattga gtgacgatga actggcacaa tttatggata acattcccgg tatggtgtta
    23581 atcaaccgcg ttgtgccggg gtacgcccat cgttgcgttt gcctggataa tctcagcggt
    23641 gcccgaatgg cgacgcgcat gttgctgaat aacggtcatc aacgtattgg ttatctttct
    23701 tccagccacg gcattgaaga tgacgccatg cgtaaagcag gctggatgag tgcgttgaaa
    23761 gagcaggata ttattccgcc ggaaagctgg attggcactg gtacgccgga catgccgggc
    23821 ggtgaggcgg cgatggttga actgctgggg cgcaatctac aacttaccgc tgtatttgct
    23881 tataacgaca atatggccgc tggcgcactg acagcattaa aagataatgg cattgcgatt
    23941 ccgttacatc tctcaatcat cggtttcgat gatattccca tcgcccgtta caccgacccg
    24001 caattaacga ccgtgcgtta tcccattgct tcaatggcta aattagccac cgaactggcc
    24061 ttgcaggggg cagcaggcaa tattgatcct cgtgccgcca ctgttttatg ccgacgttag
    24121 tgcgtcgcca ttctgtcgca acgcgccaga atgcgtcggc gatcactaac tcaacaaatc
    24181 aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc
    24241 tatgattgca ccgttttaat gttgtaaccc gtatgtaaca gtgaataatc actttcaccg
    24301 aggtaatagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag
    24361 aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg
    24421 ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc atgaataaga
    24481 aggtgttaac cctgtctgct gtgatggcca gcatgttatt cggtgccgct gcacacgctg
    24541 ctgatactcg cattggtgta acaatctata agtacgacga taactttatg tctgtagtgc
    24601 gcaaggctat tgagcaagat gcgaaagccg cgccagatgt tcagctgctg atgaatgatt
    24661 ctcagaatga ccagtccaag cagaacgatc agatcgacgt attgctggcg aaaggggtga
    24721 aggcactggc aatcaacctg gttgacccgg cagctgcggg tacggtgatt gagaaagcgc
    24781 gtgggcaaaa cgtgccggtg gttttcttca acaaagaacc gtctcgtaag gcgctggata
    24841 gctacgacaa agcctactac gttggcactg actccaaaga gtccggcatt attcagggcg
    24901 atttgattgc taaacactgg gcggcgaatc agggttggga tctgaacaaa gacggtcaga
    24961 ttcagttcgt actgctgaaa ggtgaaccgg gccatccgga tgcagaagca cgtaccactt
    25021 acgtgattaa agaattgaac gataaaggca tcaaaactga acagttacag ttagataccg
    25081 caatgtggga caccgctcag gcgaaagata agatggacgc ctggctgtct ggcccgaacg
    25141 ccaacaaaat cgaagtggtt atcgccaaca acgatgcgat ggcaatgggc gcggtagaag
    25201 cactgaaagc acacaacaag tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag
    25261 cgctggcgct ggtgaaatcc ggtgcactgg cgggcacagt actgaacgat gctaacaacc
    25321 aggcgaaagc gacctttgat ctggcgaaaa acctggccga tggcaaaggt gcggctgatg
    25381 gcaccaactg gaaaatcgac aacaaagtgg tccgcgtacc ttatgttggc gtagataaag
    25441 acaacttggc tgaattcagc aagaaataag actgattggg cgcagtcgtt tactgcgccc
    25501 ttattgtatg gccaacaagg taaaattata tggtcagctc aacgactccg tcttccgggg
    25561 aatacttgtt ggaaatgagc ggtatcaaca agtcctttcc tggtgttaag gcacttgata
    25621 acgttaattt aaaagtccgg ccacattcta tccatgcatt aatgggggaa aacggtgcag
    25681 gaaaatcgac attattaaaa tgcctgtttg gtatttatca aaaagactcc ggcaccattt
    25741 tattccaggg taaagagatc gatttccatt ccgccaaaga ggcactggaa aatggtattt
    25801 cgatggtaca ccaggagtta aacctggtat tacaacgttc ggtgatggat aacatgtggc
    25861 tggggcgata tcccaccaaa ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca
    25921 aagcgatttt tgatgaactg gatattgata tcgatccgcg tgcgcgcgtc ggcacattat
    25981 ccgtttcgca aatgcagatg atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg
    26041 tgattatgga tgaaccgact tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta
    26101 ttattcgtaa attaaaagag cgcggctgcg gtattgttta tatctcgcat aaaatggaag
    26161 aaatcttcca gttatgtgat gaagttaccg tattgcgcga cggtcagtgg atcgccaccg
    26221 aaccgctggc aggactgacg atggacaaga tcatcggcat gatggttggg cgttctctta
    26281 atcagcgttt tcctgacaaa gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc
    26341 taacgtcact gcgccagccg tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga
    26401 tcctcggtat tgccgggctg gtgggggcga aacgtaccga tattgttgag acgttatttg
    26461 gtattcgcga gaaatcggct ggcaccatta cgttgcacgg caaacagatc aataaccata
    26521 atgccaacga agccataaac cacggatttg cactggtaac tgaggagcgc cgctcaacgg
    26581 gaatttatgc ctatctggat attggtttta actcgttaat ttccaatatt cgcaactaca
    26641 aaaataaagt tggtttactg gataactcgc ggatgaaaag cgatacccag tgggtgattg
    26701 attcgatgcg ggtaaaaacg ccgggtcatc ggacgcaaat tggttcgctc tccggtggta
    26761 atcagcaaaa ggtgattatt ggtcgctggc tactaacgca accagaaata ttaatgctcg
    26821 atgaaccgac gcgcggtatt gatgtcgggg cgaagtttga aatttatcag ttaattgccg
    26881 aactggcgaa gaaaggcaag gggattatta ttatctcctc tgaaatgcct gagttgttag
    26941 ggataacaga ccgtattctg gtcatgagca atggtctcgt ttccggaatt gtcgatacaa
    27001 aaacaacaac gcaaaacgaa attctgcgtc ttgcgtcttt gcacctttaa gattaggggc
    27061 ttcccatgag tgcgttaaat aagaaaagtt ttcttactta cctgaaagag ggcggtattt
    27121 acgtcgttct tttagttttg ctggcgatta ttattttcca ggacccaaca tttttaagtc
    27181 tgttgaactt aagtaatatt ctcacccagt catcggtgcg tattattatc gcgctcggtg
    27241 tggcagggtt aattgtcacc caggggaccg atctttctgc tggtcgtcag gtagggctgg
    27301 cggcagtggt ggctgcgaca ttattgcagt ccatggataa cgccaacaaa gtgttcccgg
    27361 aaatggcgac gatgccgatt gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga
    27421 tcggtttgat caacggtctg attatcgctt atctcaacgt gacgccgttc attaccacgc
    27481 tcggcacgat gatcatcgtc tatggcatca actcgctcta ttacgacttt gtcggggcgt
    27541 cgccaatttc tggttttgac agtggcttct ctacctttgc tcagggcttt gtcgcgctgg
    27601 ggagtttccg tctctcttac atcaccttct acgcgttgat tgcggtggcg ttcgtctggg
    27661 tgttgtggaa caaaacccgc ttcggtaaga acatttttgc cattggcggt aacccggaag
    27721 cggcaaaagt atctggtgtc aacgtcggcc tgaacctgct gatgatctac gcgttgtctg
    27781 gcgtgttcta tgcctttggc gggatgttag aagccggacg tatcggctct gccaccaaca
    27841 acctcggctt tatgtatgag ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt
    27901 tcagcggcgg tgtggggacg gtgattggcg tggtgaccgg ggtaattatt tttaccgtca
    27961 tcaactatgg cctgacgtat atcggcgtaa acccatactg gcagtacatt atcaaagggg
    28021 cgattattat cttcgccgta gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg
    28081 attgcggata ttgccggatg cgctgcatcc ggcaatcggt gccaaatgcc ggatgcgtct
    28141 ctggttttac aacgttaccg ggtgttcttt ctcgcctttc ttaaacttca cttccccgag
    28201 ctcaatacaa cccaccgggc agacgtgacc acaaagcaga caacccacac atttctcggt
    28261 attacaatgc ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta
    28321 acaggaaata taacagcgtc cacagccaac acatttgtca agattgatac ggggatagac
    28381 aatataactg cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat
    28441 ctcctgcaac gaatcaaatc cctgatcggc gagataatgc gataacccgc tcgccatatc
    28501 ttctacgatg cgatagccgt actgcataat gccggtggtg acctgtaacg ttgctgcgcc
    28561 gagcaataaa aactccgccg catcttccca ggtttcaatg ccgccgatac cgctgattgg
    28621 gaaatcgcgc agttctggat gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt
    28681 taccgcttta ccggaatatc cggaaatact cgatttaccg ttaacgatcg gcataccgat
    28741 tttctgatta agatcgatat tggtgatgga tttaacggtg ttaattgccg caatgccatc
    28801 tgcgccgccg cgcttcgccg ccagcgccac ttcgcacata tcaccgatat tcggcgtcat
    28861 cttcgccagc attggcagcg tggaaccccg tttcactgcc cgacaatatt tttttaccag
    28921 ctccgggctt tgcccgacat cgctacccat cgcatgagaa gtcatttgcg gacaggagaa
    28981 gttacactcg atcatatccg cgccagcttc ttgcaccagg cgcgccagct cctcccattg
    29041 ctgctcattt tcccccatga tcgaagcgat caatactttg tccgggtaat cttccttcag
    29101 ccgacgcagg gcggtcagat tctcttccaa cggatgttca gcaatctgct ccatattttt
    29161 gaagccgata aaaccggtat cttctttcac cagatgatca aaacgcggcg agacttcgtt
    29221 ggcgataaaa aagccgatcg ttttaaacac cacaccgccc caacctgtgt cgtaggcttt
    29281 ggcacacatc tcatagcagt tgcctaccgg cgaagaagag aggcagaacg ggttgggaaa
    29341 gttcacgccg caaaaagtaa tcgaaagatc tttcgttaac atgagcaagc tccctctaat
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcgggtct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgg
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg gtcgctatgc tcaaacagcg
    29941 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30001 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30061 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30121 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30181 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30241 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30301 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30361 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30421 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30481 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30541 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30601 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30661 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaaactg tgaaaggatt
    30721 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30781 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30841 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    30901 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    30961 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31021 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31081 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31141 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31201 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31261 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31321 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31381 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31441 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31501 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31561 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31621 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31681 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31741 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31801 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31861 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    31921 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    31981 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32041 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32101 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32161 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32221 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32281 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32341 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32401 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32461 accactcaac caggcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    32521 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32581 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32641 tgcgaatgcc agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg agacttgctc
    32701 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32761 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32821 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    32881 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    32941 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33001 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33061 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33121 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33181 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33241 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33301 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33361 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33421 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33481 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33541 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33601 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33661 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33721 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33781 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33841 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    33901 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    33961 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34021 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34081 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34141 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34201 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34261 gatatacgtg tgttactggc accgatggag ggagtgcttg actctctggt gcgtgaattg
    34321 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34381 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34441 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34501 aacgccgccc gtgcggtgga gttaggttcc tggggcgtgg atctcaattg cggctgcccg
    34561 tcgaaaacgg ttaacggtag cggcggcggg gcgacgt
//
LOCUS       DAJTRQ010000027        33995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000027 DAJTRQ010000000
VERSION     DAJTRQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(17..2113)
                     /locus_tag="OO332_001436"
     CDS             complement(17..2113)
                     /locus_tag="OO332_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5941969.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2115..2366)
                     /locus_tag="OO332_001437"
     CDS             complement(2115..2366)
                     /locus_tag="OO332_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5941970.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2519..3082
                     /locus_tag="OO332_001438"
     CDS             2519..3082
                     /locus_tag="OO332_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5941971.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3358..3780)
                     /locus_tag="OO332_001439"
                     /pseudo
     CDS             complement(3358..3780)
                     /locus_tag="OO332_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(3780..4373)
                     /locus_tag="OO332_001440"
     CDS             complement(3780..4373)
                     /locus_tag="OO332_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5941972.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4520..4927
                     /locus_tag="OO332_001441"
     CDS             4520..4927
                     /locus_tag="OO332_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5941973.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5047..6039)
                     /gene="rpoS"
                     /locus_tag="OO332_001442"
     CDS             complement(5047..6039)
                     /gene="rpoS"
                     /locus_tag="OO332_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5941974.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6102..7241)
                     /gene="nlpD"
                     /locus_tag="OO332_001443"
     CDS             complement(6102..7241)
                     /gene="nlpD"
                     /locus_tag="OO332_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5941975.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7381..8007)
                     /gene="pcm"
                     /locus_tag="OO332_001444"
     CDS             complement(7381..8007)
                     /gene="pcm"
                     /locus_tag="OO332_001444"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5941976.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8001..8762)
                     /gene="surE"
                     /locus_tag="OO332_001445"
     CDS             complement(8001..8762)
                     /gene="surE"
                     /locus_tag="OO332_001445"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5941977.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8743..9792)
                     /gene="truD"
                     /locus_tag="OO332_001446"
     CDS             complement(8743..9792)
                     /gene="truD"
                     /locus_tag="OO332_001446"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5941978.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9789..10268)
                     /gene="ispF"
                     /locus_tag="OO332_001447"
     CDS             complement(9789..10268)
                     /gene="ispF"
                     /locus_tag="OO332_001447"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5941979.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10268..10978)
                     /gene="ispD"
                     /locus_tag="OO332_001448"
     CDS             complement(10268..10978)
                     /gene="ispD"
                     /locus_tag="OO332_001448"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5941980.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(10997..11308)
                     /gene="ftsB"
                     /locus_tag="OO332_001449"
     CDS             complement(10997..11308)
                     /gene="ftsB"
                     /locus_tag="OO332_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5941981.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11502..11825)
                     /locus_tag="OO332_001450"
     CDS             complement(11502..11825)
                     /locus_tag="OO332_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5941982.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11875..12480)
                     /gene="cysC"
                     /locus_tag="OO332_001451"
     CDS             complement(11875..12480)
                     /gene="cysC"
                     /locus_tag="OO332_001451"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5941983.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12480..13907)
                     /gene="cysN"
                     /locus_tag="OO332_001452"
     CDS             complement(12480..13907)
                     /gene="cysN"
                     /locus_tag="OO332_001452"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5941984.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13909..14817)
                     /gene="cysD"
                     /locus_tag="OO332_001453"
     CDS             complement(13909..14817)
                     /gene="cysD"
                     /locus_tag="OO332_001453"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5941985.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15069..16106
                     /gene="iap"
                     /locus_tag="OO332_001454"
     CDS             15069..16106
                     /gene="iap"
                     /locus_tag="OO332_001454"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5941986.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16252..17343)
                     /locus_tag="OO332_001455"
                     /pseudo
     CDS             complement(16252..17343)
                     /locus_tag="OO332_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(17537..17689)
                     /locus_tag="OO332_001456"
     CDS             complement(17537..17689)
                     /locus_tag="OO332_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5941987.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(17953..18687)
                     /gene="cysH"
                     /locus_tag="OO332_001457"
     CDS             complement(17953..18687)
                     /gene="cysH"
                     /locus_tag="OO332_001457"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5941988.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(18761..20473)
                     /gene="cysI"
                     /locus_tag="OO332_001458"
     CDS             complement(18761..20473)
                     /gene="cysI"
                     /locus_tag="OO332_001458"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5941989.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20473..22272)
                     /gene="cysJ"
                     /locus_tag="OO332_001459"
     CDS             complement(20473..22272)
                     /gene="cysJ"
                     /locus_tag="OO332_001459"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5941990.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22591..22953
                     /gene="queD"
                     /locus_tag="OO332_001460"
     CDS             22591..22953
                     /gene="queD"
                     /locus_tag="OO332_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5941991.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            23031..24302
                     /locus_tag="OO332_001461"
     CDS             23031..24302
                     /locus_tag="OO332_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5941992.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24293..24553
                     /locus_tag="OO332_001462"
     CDS             24293..24553
                     /locus_tag="OO332_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5941993.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24570..25145
                     /locus_tag="OO332_001463"
     CDS             24570..25145
                     /locus_tag="OO332_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5941994.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25293..26153)
                     /locus_tag="OO332_001464"
     CDS             complement(25293..26153)
                     /locus_tag="OO332_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5941995.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(26150..26929)
                     /locus_tag="OO332_001465"
     CDS             complement(26150..26929)
                     /locus_tag="OO332_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5941996.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26907..28316)
                     /locus_tag="OO332_001466"
     CDS             complement(26907..28316)
                     /locus_tag="OO332_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941997.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28338..29792)
                     /locus_tag="OO332_001467"
                     /pseudo
     CDS             complement(28338..29792)
                     /locus_tag="OO332_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29862..30648)
                     /locus_tag="OO332_001468"
                     /pseudo
     CDS             complement(29862..30648)
                     /locus_tag="OO332_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            30967..32244
                     /locus_tag="OO332_001469"
     CDS             30967..32244
                     /locus_tag="OO332_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5941998.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32271..33741
                     /locus_tag="OO332_001470"
                     /pseudo
     CDS             32271..33741
                     /locus_tag="OO332_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         8181 a         9016 c         8382 g         8416 t
ORIGIN      
        1 cggactgttt ttttattcag gccagcggat catcttcttc ctgcatggca aaaagttttt
       61 gcatccccag ccggaaatcg cgttcaaatt cttcgtcatg ctcttcttca tattccggtt
      121 gctgaactgc caccacatta ccaaccgggc gataaacatt actcagccag ggttcagcct
      181 gtttgtgttc cagaatctga ataccttcat cttcagcatc acgaattttt tcctcagcgc
      241 gtttgcgcat tcctttaata cgctgctgtt gtttgtgata ttcagcactg acagcaaagg
      301 ctgcgcgttt attgccgttc catatcgcct cacagataaa gctgccgtcc ttgcgacgta
      361 ccgtaattcg ctcggcgtca tgaatgtcat aactgataag cactttgcga ccgtgttcgt
      421 cacgcaactc cggcgcgtag taaatattgt tcagccactg aatttcgcag cgtcttaccg
      481 gtcgttccac cattgggcgg aacatgtcgc gcagctcaag atcactcagc cactcaattt
      541 cggtgtcttc ttctgccagg cgtttttttc tgaactctgt cgggctgtaa tgccgtccgt
      601 tcggtttcat cggcagctca gaatgcgggc ggttgttgta ccattccacg ccttcacgaa
      661 tagcctcaat aagctccgcc cacgatggca gctcgcgcat tgccgctttc tggcggtcgt
      721 tcagaggctt gtcctgttgc agggcgttaa acgctgaacg cagatcacgg ttaagtacac
      781 gcaacgattc ccggtcagca cccttgccaa aataggtgcg atacctcctg gctatacgca
      841 tcggcagtgt ccggttcagg cgttcaataa tgccgcgtcc ctgtggatta ccggcaatac
      901 cggtagggtg tttaatcccc aggcgtggca gaatacccac gacctctttg tccagcatgt
      961 cagccgtttc accggaaccg ttatccgagt aatacaggaa cggttttccg tggttgcgaa
     1021 tgccgtgctg tatcgcaccg gcaacggcga acacgttttc ggcaaggtca agactccagc
     1081 caacaacaaa ccgtgttcca ccatcgatca caaacgtgac ttccggtgag aatggacgcc
     1141 cgtggatcgg atgcgcacat ttcagcttca taccgtgacc gtcaccaatc cagacataat
     1201 tcaccggcat agctgtccag tcgcggcggg tgaatccttc aagctgacgg tattcactgc
     1261 cggtaacgcg ccctttttgt ttcaccactt ccggcagttt tttcatggca taccggacaa
     1321 catcatagga aggcatcacc tccagcatgt acggttcgtc cgcatggcga cgctgccatt
     1381 ctgcgacaaa atcctcgtag gcttctgaca tgggtctgcc gttcacctgg cgatactgcg
     1441 ccagaaattc aggcagccag ctgatttctt ccggttttat ttcgtcgcgt tttcccggtg
     1501 ccagtaacag cagccgttcg gcggcgttct gcgactggtt gtatttcaga acccactggt
     1561 aaagtggatc gcggctgata gtacgattag cgccacgttt tgcattggcg ttaactgcgg
     1621 cctgttgcag gcgctccggc agctcacctt tacgggcttt ttcaacaaga aagttaatgg
     1681 ctttcagtcg cccgaggtta ccggcgcttt ccagcttcag cacttccacc accagcgcag
     1741 ctcttgcttc agatacccag cgctggtttt cggtcagcgc attcaggcgt tcttccatca
     1801 gttgtggaga accgcgatat gcttcgattg cctggacagc gtcaggattg cgtgtttttc
     1861 tggtaacagc tttcacttca gaaacattgt tctctgtagc caccagctgt tccacatagc
     1921 gttcacgcag tgcctttcgt gttatttcag gcagacaatc aatgtggtac tcaatggctt
     1981 ttgtgccttc ccgacggcga ctaacgtcac caagatcacg agaaaaacgc tgtaacgcct
     2041 gtcggatgcc ttttgtggtg gctggcattc ctggtacgcc gagcaggtca tttacggtaa
     2101 cgtacgactg catatcacga cgccttcctg ataaatccat cgttacgata ccggctgggc
     2161 cagatgattt ctggcggtac acctatagcg tcagaaatta cgcgttcgta ttttttgttt
     2221 ggtgtgcgaa ctaccgcttt cagtgaatcc tgagcataac cagcatggag tgaaagctcc
     2281 cggaaggaag accacccgtt atctttgagc gccagctgaa tcttcagcga aggccaatcg
     2341 cggtcactat cttctctttg tctcatcttt cctttaacct acaaaataac ccgcgcggat
     2401 atccgtatgg ataaagatta agataccaaa gattccctat aaagggttct ttggtgcttt
     2461 tatggtttat tttttaattt cattatttat caatgaatta gatagaaagg aaccaagaat
     2521 gagaaaagca ccccaaggtt cttttcctgc cgatggaaaa gaaccgatta tcgaaagaat
     2581 ctttaggtta gtagaacgat acccttcaag aaatgcggct gctcgggcat gggggatcaa
     2641 tgaaggcact ttaaaaaatt attacaacag gcgggattac gcaccgatac cacgcccgca
     2701 tcagttaaaa aagatagcag aaagcgaaaa tgttaccttg gaatggctca tgacgggaga
     2761 gggtgatgtt gaactaaaag attccaaaga acccaaaaaa gagaatgcgg ataatgttga
     2821 tgctcaaatt ctaatgttct tgtcatttct taaaccacaa gaaaaacaac aagtagctga
     2881 tgttcttggg agaaaaggag cggagcaatt actcattctt ctggatgagc acatccaaga
     2941 gttgcacgct ctagttggag ttcgtagagc catagcgtta agccttggta atttgccaga
     3001 cgaaaaggtt agagagattt acgcgttgtc cgaagctaag ggcgatcact taaatctacc
     3061 tcagaaagca gccagcgcat aacccagtgc aagtgcctag ctgtgctaaa attacattgg
     3121 ttttggcgct tgcacccatc agcgctataa taaacacctg aatcgttttt gaacggtctt
     3181 taaaggctat tgtaagaacc tgtgagttat cttaaatgtt ttattgatat cgaaccgttt
     3241 aacatttttg gactcgcttt atcttgaaaa ccttgagttc ctaacttgtt gattccaatc
     3301 tattcccgtc ttttttaatc tcttcccggt tatcttgaat aaattaccta gtcatacgag
     3361 atagaaaccg ccatcgccat cattcaggcg aaacagcggc agaatatcga acaggttgat
     3421 ctcttcgcca tcgacggtgt tctgcgccca ggctggattg gcgcggcgct ccggggcaat
     3481 cgggaagtta tcccagcggc ggataaactc atcaatctgc tttttgaccg gggtgtttgg
     3541 tggcaggcca agggaaatcg cgtggttctg ccaggaaccg atggtattca tcgccacgcg
     3601 ggcatcggta aagccgcgaa tattatcaaa ccacagcgcg ggcgcgccgt cgccgatacg
     3661 cccggtggcg ttagctgctg ctgccagatc cggctcagcg ttcacttctt cgctgatttt
     3721 cagtaactgg ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat caaatgccat
     3781 tattcattct cctgagaaaa attccgggcc tgcggcaatc cttgccagcg cctggcgtgg
     3841 ggatgttcga ggccaaattg atccagcacg cgggctacca catggtggac aatgtcatct
     3901 accgtttcgg gatggttata aaaggcaggc atcggcggca ccatcgccac gcccatgcgt
     3961 gaaagtgcga gcatattttc gagatggatg gtgctaagcg gcatttcacg cggcaccagc
     4021 accagtttgc ggccttcttt gagcacgacg tccgccgcgc gccctaccag gccatcggcg
     4081 taaccagcgc ggataccggc gagcgttttc atactgcacg gaataacgat catgccgtca
     4141 gtacgaaaag aaccggatga gatgatcgcc gcctgatccg ccgggttatg gctgaagtct
     4201 gcgagggcag caacatcgcg agcgctgtaa ggcgtttcta gttcaatgat ggttttcgcc
     4261 cacttcgaca tcaccagatg agtctcgaca ttcggcatct cacgcagcgc ttgcagtaat
     4321 gccacaccaa gaggcgcacc ggtagcccct gtcatcccga cgatcagttt cattgcttat
     4381 tctcttaatt gttcgtatac gaacattatt attaacatac tccttcattt ccacagcggc
     4441 aaggattgaa aagtaacaac atcttcttcg tcactaaaaa accgctatga tagcgacaga
     4501 gaattagccc ggagtttaca tggcgttacg aaataaagcg tttcatcagt tacgacagct
     4561 ttttcagcag cacacggctc gctggcagca cgagttacct gacctgacca aaccacagta
     4621 cgcggtgatg cgcgccattg ctgataaacc tggtattgaa caggtggcac tgatagaagc
     4681 agcagtcagc accaaagcaa cgctagcaga aatgttggca agaatggaaa atcgtggctt
     4741 agtcagacgt gaacatgacg tcgctgacaa gcggcgacgc tttgtctggc tgactgccga
     4801 aggagaaaag ctacttgctg cggcgatacc tattggtgac agcgtggatg aggaattttt
     4861 ggggcgtttg agtggagaag agcaggagct gtttatgcag ttggtgcgca agatgatgag
     4921 cacatagaat gctgattgcc gggtaggacg ctgacgtgtc ttatccaggc gacaaaaaca
     4981 caagcttccc aaacaaaaga aaaaggccag cctcgcttga gactggcctt tctgacagat
     5041 gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca ggatttcgcg
     5101 caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga ggccaatttc
     5161 acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag
     5221 tacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct gcttcatatc
     5281 gtcatcttgc gtggtatctt ccggaccgtt ctctttttca tcggccagga tgtccagcaa
     5341 cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct cgttaagacg
     5401 aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct cttccgcact
     5461 tggttcatgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc
     5521 ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg cccgttcaat
     5581 cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa
     5641 cttctctacc gcgcggatca gccccaagtt gccctcttcg ataaggtcca gcaacgccag
     5701 accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac tctcgatcat
     5761 ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc
     5821 ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg cgtccaacac
     5881 acgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat cactgggttc
     5941 ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc
     6001 ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc tacccgtgat
     6061 cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg cggcaaataa
     6121 cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg
     6181 gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg ttgttcccgg
     6241 accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg tttgatgata
     6301 atcagattac cgtagccgcg cagcgcgtta ccagcataaa caacgcggcc atctgcggtc
     6361 gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa
     6421 gcgccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga gataggcgta
     6481 ctggttgatg tactgctgac agtcggctcg gttgtgcttg ctgttggtac cgttacaggc
     6541 gctgtgaccg tggtcgcagt tggcttgttg ttcggcaaca ttttgttagc actctgttca
     6601 cccgaagact cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt
     6661 gcaggcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatagc atttccgcca
     6721 gtgattggcg taccggaagc attacccacc tgcaaggtct gaccaacgtt cagcgcgtat
     6781 ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt aatccaggcg
     6841 atatagaaaa gtgtgtcgcc ttttttcacg gtataggtac tgccgctata actgcctttc
     6901 ggaatgttcc catactgacg gttatagacg atgcgtccgt tttccatctg taccggctgc
     6961 tgagctactg gctgcactgg ctggatttgc ggttgttgag tagcctgaat ttgtggctgc
     7021 tgtaccggct gaatttgcgg ttgctgcgct gtagacgtcg tccctatttt cggcggcggc
     7081 gtaatcaaca taccagaatt agtatttgca ggcgcattgc cattaacgga gctgaccggt
     7141 gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag tgacaaagcc
     7201 gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc aggaaaaatt
     7261 ggttaataac cagtgacata attaccgtgc aaggcaccct actgaacact ggaaaagatg
     7321 ttcacgatac gctgacctac ggcaaaataa ccaggaaaaa tccaggtatt tcctcacgtt
     7381 ttaagccagc tcacccttca ctaaagggac aaagcgcacg gcctccacgg tatcgataat
     7441 aaattcgcct ccccgacgac gcacccgttt caaatactgg tgctcctccc ctacgggtaa
     7501 gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt ccggcggtgc
     7561 cgccgtaaca atgatagcgt caaacggcgc acgtgcctgc caaccttgcc atccatcacc
     7621 atgacgggtt gaaacattat gtaaatcaag atttttcagg cggcgacgtg cctgccactg
     7681 caagccttta atccgttcaa ccgagcaaac atgctggaca agatgcgcca ggattgccgt
     7741 ttgatatccc gaaccagtgc caatttccag cacccgcgac tgcggcgtca gctcgagtaa
     7801 ttcggtcatt cgcgccacca tatacggctg cgaaattgtc tgcccctgac ctatcggcaa
     7861 ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt cacgcggcac
     7921 ggcggcaagt gcattcagca cctgctcatc ctgaatacct tgcgcacgta attgatccag
     7981 aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt taaccagtct
     8041 gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt gatggagaca
     8101 tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt accgcccggc
     8161 gggccaatcc agtacagcgt attgccgcgc ggatcttgct gcgggatcac ctgatctgcc
     8221 ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa gggtaaatcc
     8281 ggaacgttaa tattaagaat acgtccggtg cgcagcggct ctttacacag tgcgcgcaaa
     8341 attgaacagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag cgagacggcc
     8401 agcgccggaa aacctaaatg acggccttcc atcgcggcgg ctaccgtacc ggaataaata
     8461 acatcatccc ccagattcgg cccggcgtta attccggaca caacaatgtc cgggcgcgga
     8521 cgcatcagag cattcacgcc aagatagaca caatcggtcg gggttcccat ttgcacagca
     8581 atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag agaatttgaa
     8641 gcgctgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc acgcaaggct
     8701 ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag caatatgcgc
     8761 ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac tacccgccgg
     8821 aagccagaaa cggatctcta cggtgacgtc atcccaccag ttccagctta attgttgcgg
     8881 atacagcagc atcgctctgc gcgcggcttc aactttttcg cgcaccagta aagcttgtaa
     8941 ttcagtttct gcggcgacag ctgcttgttc gaatgccagc gcttcacgct gagttcccca
     9001 ttcgccactg cccggcaatg cggcggttat catcagctct ttatcgttga cgcgacgctg
     9061 taattccgcc agttcttcgg tggttgctac aaaccagcta ccacgtccgg ctaattgtag
     9121 cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgctcag caacaatctg
     9181 attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc gatcgcgcac
     9241 cggagtattg gtttgcgccc agcgcagcgc gccctgcaag ttgctaccgc caatcccaaa
     9301 acgttgggca ccgaagtagt tcggtacacc ttttgcgcaa atatcgatca gacgttgttc
     9361 aacgtcatcg cgattgctca cttcgcgcag aaccagggta aaggcgttac ctttcagcgc
     9421 ccctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc cttccagttg
     9481 aaaggcgctc agatcgggca tttccttgcc cggcacgcga gcacataacc actgttccgt
     9541 tacagcatgt ttgtcttttt gcccagcgaa gctgacttca cgggaatgaa ttttcaggaa
     9601 tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga ttctaaccag
     9661 aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca caaagtcttc
     9721 cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag tgagattatc
     9781 aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc aatcccttcc
     9841 ccacgtccgg taaatccaag tttttccgta gtagtggctt tcacgttaac atcatccata
     9901 tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg caacatcttc
     9961 ggtgcctgag cgatgatagt gacatcgacg ttgccaaggg tataaccctt cgcctgaata
    10021 cgacgccagg cttcgcgtag cagctcgcgg ctgtcggcac ctttaaatgc cggatcggta
    10081 tccgggaaca gtttgccgat atcccccagc gccgccgcgc caagcaatgc atcggtcaac
    10141 gcatgaagcg ccacgtcgcc atcagaatgc gccagcaatc ctttttcgta aggaatgcgt
    10201 acgccaccaa tgataattgg gccttcaccg ccaaaggcgt gtacgtcaaa accgtgtcca
    10261 attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaactcg gccagtgcca
    10321 aatcttccgg gcgcgtgact ttaatgttat ccgcgcggcc ttcgaccaac tgcggatgaa
    10381 atccgcaata ttccagcgcc gaggcttcgt cggtaatggt agcaccttca tttagagcgc
    10441 gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc gcgtgccata
    10501 agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca cgtttcatag
    10561 tatcgcgcac tggtgcggcg aggatccctc ccgtgcggct ggtttcgctc aacgccaaca
    10621 atcgcgcgag gtcatcctga tgcaaacaag gacgagcggc gtcatgcacc aatacccact
    10681 gcacatcgcc agcggctttc agacctgcca gcacggaatc ggcacgctca tcaccgccat
    10741 ctacaacggt gatttgcgga tgattcgcca gaggaagttg tgcaaaacgg ctatcgccag
    10801 gacttatggc aatgacgaca cgtgtcaccc ggggatgcgc cagcagcgca tgcaccgagt
    10861 gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc gtttgcattc
    10921 gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg gttgccatgt
    10981 taattcccgg gctgatttat cgattgtttt gccccgcaga ctgtgcgcgc ttcgacgcgt
    11041 caggcaccag acgataaaaa gtttcgcccg gcctggtcat gctgagttca ttacgcgcac
    11101 gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa agttgatcgt
    11161 ttcgcgcttt aagtttcgcg tttgtagctt gcagtgccgc cacatcatca ttgacgcggg
    11221 tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag accagaatag
    11281 ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaactgcat catcatccca
    11341 tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc gggcaaatgt
    11401 accacatttg tccattgtta cgtataccca gggcgtgcag aacataatct cattattagt
    11461 tacggtttga attatgaaca gaggagacaa gaaagtacaa attagcccag tagccacata
    11521 aacagtgcgc caaacataat gcctactgtc accagggtga aaacaatact gtagcgtagc
    11581 tttccgtcca tcaatgaatg cagcgcaatc cccaccacta ccgcgacagg catcagtgcc
    11641 agaaagaaag gccaggtgta gataaagaag aacagcgtgt tagagccata aatcaacatc
    11701 ggcatcgcca gcgcaaataa ccaggagata aaaccgacca cggcaccagg cagtgaccat
    11761 gtagtttctt catcctcagt aaggctgtcg ttatttgtta gtgtaatgtt atggctatta
    11821 cgcatatttg atcctgttac tttgacgaac cgggcattga aacccggtgg tgtctcagga
    11881 tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat ttgttactaa
    11941 ttgttcacca ttgagatgaa tttctgccga ttcaggctct tcgtaaacgg aatctattcc
    12001 cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aagccttttg gatcgcgggc
    12061 ttcgcaaatc gccagcggcg tatcgacaaa cacttcaata aagcgccctt ctcctacgcg
    12121 ttcgcgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca gcaccaccag
    12181 tccggctgca accatcaaat tcgccacttc accgacgcga cggatattct ctttacgatc
    12241 ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acattgtcgc catccagcag
    12301 atacgtactg acgccgagtt tatgtaacgc ctcctccagc gccccggcga ccgttgattt
    12361 accggacccg gagaggccgg taaaccacag cactacacca cgatgaccgt ggtgtagttc
    12421 gcgttgttgc acagtgaccg gatgactatg ccagacgacg ttttcgtcat gcagcgccat
    12481 tatttatccc ccagcaaatc gcgcgcgccc cagtgcggaa agtggcgacg aaccagggca
    12541 ttcaattcca gttcgaatgc actgaattca gatgacgcag ctgttgcctg gctaactggc
    12601 tcgtgcacca taccggcacc cacggtcaca ttgctcaggc gatcgataaa aatcagccca
    12661 ccggtaaccg gattttgctg atagcgatct aacaccagtg gctcgtcaaa agtgagatcc
    12721 accaggccga tgccgttcag cggcaggttt tcaacttcgc gttgggtaag gttattaata
    12781 tcaacctgat aacgaatgcc atcaacacga gcacgcgtct tcttaccggc aattttgatg
    12841 tcataactct ggcccgggga aagcggctgt tccgccatcc ataccacatc caccgacgca
    12901 ctctgcaccg ccggtaacgc ttcgtctgcc gccagcagca gatcaccacg gctgatatcg
    12961 atctcatccg tcagcaccag ggtgatcgct tctccggcaa agacttcttc gcgatcacca
    13021 tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcac ttttacacgt
    13081 tgcccgactt ccacgcgacc ggatgccagc attccggcgt agccacgaaa atcgagattt
    13141 gggcggttaa cgtactgcac cgggaagcgc attggctggg catccaccac tcgctggatc
    13201 tccacggttt ccagcacttc gagcagtgtc agaccgctgt accacggcat actttcactt
    13261 tgcgaagcca cgttgtcacc ttccagtgcg gagagcggca caaagcggat atccagatta
    13321 cccggcagct gcccggcaaa ggtcagataa tcttcacgaa tacgggtgaa cgtctcttca
    13381 ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat ccccaacagt
    13441 gtggagataa aactgtgacg acgggtttga tcgagcacgc ctttacgggc atcaatcagt
    13501 aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgagtgta ctgctcgtgc
    13561 cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaatagcg gtaggccaca
    13621 tcaatagtga tgccctgttc gcgctcagct tgcaggccgt ccaccagcag agccagatcc
    13681 agcttttcgc cctgggtgcc gtgacgctta ctgtcgttat gcagcgatga gagctgatct
    13741 tcgtagattt ggcgggtatc gtgcagcaga cgaccaatca gggtactttt gccgtcatcg
    13801 acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat catccaggct
    13861 tcgacgccgc cttcattggc gatttgttgt gcaagtacgg tgttcatctt aaaaataccc
    13921 ctgacgtttt ttcagctcca tggatcccgc ctggtcgcgg tcaatcacgc gaccctgacg
    13981 ttcactggtg gtggaaacca gcatctcttc gatgatttcc ggcagtgttt gtgcatttga
    14041 ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg aaacgcacca tccgtttttt
    14101 gatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca acataccgtc
    14161 gcgttccaga accggacgtt ccgcagcgag atacagcggc acaatgtcga tattttccag
    14221 ccagatgtat tgccagatat cctgctcggt ccagttagag agcgggaaga cgcggatgct
    14281 ttcgcctttg ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct gatttttcgg
    14341 atcccagcga tggaagcggt cacggaaaga gtagattcgc tctttagcgc gggatttctc
    14401 ttcgtcacgg cgcgcaccac cgaaggcggc atcaaaaccg tatttgttca gcgcctgttt
    14461 caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgcacga atggattaat
    14521 ccccatcgcc acgccttccg ggtttttatg caccagcagc tcgcagccgt aggctttcgc
    14581 cgtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcga catgcagcaa
    14641 cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca tgacgctgga
    14701 atctttaccg atagagtaga gcatcaccgg atttgagaat tctgccgcca cctcgcgaat
    14761 aatgtggatg ctttccgcct ccagttgccg caggtgagta agtcgttttt gatccataac
    14821 cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata gggagcgtaa
    14881 gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa cgatttggca
    14941 aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa gatttgaaat
    15001 tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat ggtatttaag
    15061 gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc ggcgtatgct
    15121 ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat acccaggcac
    15181 gacatattgc tactttcttt ccgggacgca tgaccggaac tccagcagaa atgttatctg
    15241 ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt cggacattta
    15301 atagtcggta tatttatacc gcccgcgata atcgcaagaa ctggcataac gtgacaggaa
    15361 gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcatcatt atggcgcatc
    15421 tggatactta cgccccgctg agcgatgctg acgcagatgc caatctcggc ggactgacgt
    15481 tacaaggaat agatgataac gccgcaggtt taggtgtcat gctggaactg gcagaacgcc
    15541 tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc gaagaggaag
    15601 gaaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag aaaaatacgc
    15661 tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc aacagcggtg
    15721 taaaaacccc tgaagcagta aggaaattaa cgcgcgacag ggcgctggca attgcacgca
    15781 gtcacggaat agccgcaacg accaatccgg gtttgaataa aaattatccg aaaggcactg
    15841 ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt tcggtggaag
    15901 cgactaactg gaatcttgga aataaggatg gttatcagca acgcgcaaaa acacctgcct
    15961 tcccggcggg aaatagctgg catgacgtaa gactggataa tcaacaacat attgataagg
    16021 cacttccagg aagaatagaa cgtcgctgcc gtgacgttat gcggataatg ctaccgttgg
    16081 tgaaggagct ggcgaaggcg tcttgatggg tttgaaaatg ggagctggga gttctaccgc
    16141 aggggcgggg aattctaagt gatacccatc atcgcatcca gtgcgtccgg tttacttccg
    16201 ggaaaaccgc accgtattgc gccggtttat ctccgctgga gcggggaaca cctctggcga
    16261 atagaaagca agccaggttt tgaccagtcg atcgtacatg gcattggccg ttgccattgt
    16321 tggcaggcca aaaaacagcc cctgagcgtt acccgcagcc atcaacctgt gcgccaggat
    16381 aagcgccgct tctgttttac ctgcgccggt cacatcttcc agaataaaca actgtggtcc
    16441 tggctgattg atatccagat ccagcacctt ttgctgtaac ggtgtcgtat tctcaataaa
    16501 aggaaacagc gtattaattc cggtgaaagg cgcggtttct gcttgtggcg gaaagacggt
    16561 taatgcgttt tgagcctgaa ctaaagcctt ttgccagtaa tcttcaatat ctattgcctg
    16621 tgcgacgcgt ggaaaaaatc gcgttgacga ccctgtccag tctgcaagta cgacggttgc
    16681 agagatatac caggaaagtt gttttataag ttcaacgccc tcgtcatcat cccagaatgt
    16741 ggggatctct attcgcggaa acagtggctt gatttcaagg agaaaatctc gtgctgcagc
    16801 tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggcc gaccatgatg
    16861 ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg gagagagcga
    16921 agatgaagga agtaactcct gacattcact taaataatga ttccacaacc agtagcccag
    16981 cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct tgaattcagg
    17041 gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc agcacagcaa
    17101 ccaggcgaaa aattgagctg cctgttcctt gtcagaaatc cctaattgac gaaaataatc
    17161 agccagcccg aagcaatttc ttttaaccat taaatagccc attgcggcca catccagcaa
    17221 atgccagcaa agaaggtgat agtcgtcgct accctctttc tcgccacgtc ggttttttcc
    17281 ccagaaatca aagaaagtca caatattttt atccttcagt aaacttaaag gatatttacg
    17341 catattttaa aaactatctg tgatatatat cagccaataa aacatttaca ttatgaataa
    17401 attaatgatt ttcatttgaa attcataata ataaatagag actatatatt cacataaaaa
    17461 atataaattt taaaaaaggg tgcaggagac caaaccatca caattcagta cgcggggttt
    17521 tacccccgcg tggtggctac ttcttcgatt cgtaagcgag aactgcctta aactccatac
    17581 cacgttctct gatactcaac tcacataacg agtcacctac catcaacgtg aatcccagta
    17641 ctgtacaaca cagtacgatg attgccacaa gggcatattt tgtcagcatg tcttgcctcc
    17701 ctgcaaagaa gagactaaca tccgaattgc tacgttcgaa cgcgagcctc aggttgatag
    17761 aaatatcgcc tggggctttt gtccatctgg aacctcgcga atgcttaacg ccagacagcc
    17821 tcaagcaccc aacgccattc tatacctgtt aattttccct gcgatgttca cctttaaatg
    17881 ccggaatgct gctcgcaaaa taaaaacgcc cccatcattt ctgacagagg cgtttaattt
    17941 atccggcaat atttaccctt cgtgtaatcc acattccctt tttaagccaa agaaacgcgt
    18001 ttcttcttcc accatgccgg gttcccattt acgggttgta tgggtatcgc ccaccgataa
    18061 atatccttcg tcccataatg ggtgatattt caggccatgt ttttgcaggt actgataaat
    18121 agttcggtta tcccagtcga taatcggcag cactttaaat acgccacgct gaattgccag
    18181 caccggtaaa ttggcacggc tgccggattg ttcacgccgc aggccagcaa accacgtttg
    18241 cgcattcagt tctttcagcg cccggttcat cggttcgact ttgttgatgt cattgtactt
    18301 ttcaatgcct tcaacgccct gttcccacag tttgccgtag cgtgcttcct gccaggcggc
    18361 gctttcggta gcgcggtaca ctttcaggtt gagcttgagt ttgtccgtta actcgtcaat
    18421 aaagcggtag gtttccggga acagatagcc ggtgtcggtg aggatcaccg gaatatccgg
    18481 gtgaatctga ttcaccagat gcaggctcac cgccgcctga atgccaaagc tggaagaaag
    18541 tacatattca ccgggcagat tatccagcgc ccaggcaacg cggccttcag cgtccagttt
    18601 ttccagttcg gcgttagttt ctgccagcgc cagaatgcga tctaccttcg gcagttcgtt
    18661 cagggcgttt agatcgagtt tggacataga ttcctcactg tttgccttgc ctgatgcgac
    18721 gctcacgcgt cttatcaggc ctacaagacc gggctgatgg ttaatcccac aaatcgcgcg
    18781 ccggatcgag caccgggcga atgatgcccg cacgcaccgt aaagtcgccg aagccttcac
    18841 ccgcttcgcg ctctttcgcc cagcgcccta tcagttcatc aagcgacgcc aggatttccg
    18901 gctcggtgat gttttcttta tacatccgtg ggatacgtgt cccaatgcgg ttgccgccaa
    18961 gatgcaggtt gtagcgaccc ggtgctttac ccaccaggcc cacttccgcc agcatcgcgc
    19021 gaccacaacc gttcgggcag cctgttacac gcatcacgat atgctcatcg ctgacaccat
    19081 gtttcgccat taaattatcg atgttgtcga taaaagacgg caggaaacgc tccgcttccg
    19141 ccatcgccag cgggcaagtc gggaatgaca cgcaggccat cgagttttca cgctgcggcg
    19201 tgacggcatt cattaacccg ctctctttgg caattttctc gatcttcgct ttctcgcttt
    19261 ccggcacacc ggcgatgatc agattctggt tcgccgtaat gcggaaatca cctttgtgga
    19321 tcttcgcgat ctccagcagg ccagttttca gcggacgccc cggataatca aggatgcgac
    19381 cattttcgat aaacagcgtc aggtgccagt tatcatcaat gcccttaacc cagccaatac
    19441 gatcgccgcg tcctgtaaat tcatacgggc ggatcggttc aaatttgatc cccgcgcgac
    19501 gctccacttc cgctttaaac gtctcaaccc ccacgcgctc cagtgtgtat tttgttttgg
    19561 catttttacg atcggttcgg ttaccccagt cacgctgagt tgtcacgacg gcttccgcca
    19621 ccgctagcgt atgctctagc ggcagatagc caaactcact cgccgtgcgg gcgtaggttt
    19681 tcttgttacc gtgttcgata gaaagcccac cgcccaccag caggttgaag cccaccagct
    19741 tgccgttttc cgcgatcgcc acgaagttca tgtcgttggc gtgcagatca atatcgttct
    19801 gcggcgggat cactaccgtg gttttgaact tacgcggcag gtaggtctga ccaaggatcg
    19861 gttcttcatc ggtggtggcg actttttcct gatcgagcca gatctccgca tacgcgcggg
    19921 tacgcggcag cagatgttca gaaatcttct tcgcccactc gtacgcttct gcgtgcagct
    19981 gcgactcgta agggttcgag gtgcagagta cgttacggtt catgtcgtta gcagtcgcca
    20041 gcgcatcaag accgaccgag tgcagcatct ggtgcaccgg tttgacgttc tttttcagaa
    20101 tgccgtggaa ctgaaacgtc tggcggttgg tcaggcgaat gctgccatag atggtgtttt
    20161 caccggcaaa tttatcgatc gcctgccact gtttggtggt gataactcca cccggcagac
    20221 ggcagcgcaa cagcatcgcg tggcgcggct ccagcttctg ttcagcacgt tcggcgcgga
    20281 tgtcgcggtc atcctgctga tacatgccgt ggaagcggat cagcaggaag ttatcgccct
    20341 taaagccgcc ggtcagaccg tcgtttaaat cttccgcaat ggtgccgcgc aggtagttgc
    20401 tttcaagctt catgcgctcg gcgtctgtca gttttccttc gaccactaaa ggccctggat
    20461 gtttttcgct cattagtaga catctcgctg ataacggcgc tctacgcgca gctcacttaa
    20521 aaattcatcc gccgcttcgg tgtccatgcc accaaattcg gcaatcactt ccagaagtgc
    20581 ctgctcaacg tctttcgcca tgcgattagc gtcgccgcag acataaatgt gggcaccgtc
    20641 attgatccag cgccacagct ccgcgccctg ttcgcgcagt ttatcttgta cgtaaacttt
    20701 ttctttttgg tcgcgcgacc aggcgagatc gatccgcgtc agcacaccct ctttgacgta
    20761 gcgctgccac tccacctgat acaggaagtc ttccgtaaaa tgcggattgc caaagaacag
    20821 ccagttttta cctggcgctt cgtcagcggc gcgttgctgc ataaaggcgc ggaacggcgc
    20881 aataccggtg cctgggccaa tcataatcac cggggtttcc aggttagcgg gcaggcggaa
    20941 gttatcgtta tgttcgataa atacgcggac ttcgccctct tcttccacgc ggtcagcgag
    21001 gaagctggag gcaccaccgg cacgggcgcg gccttccacg tcgtaacgca ccacaccaac
    21061 ggtgacgtgt acttcgttct cgacttccgc ctgcgaggag gcaatggaat agagacgcgg
    21121 ggtcagcggg cgcagcagat taattagcgc ttcggcatca agctgtgccg gggagaaacg
    21181 caccatgtca acaatcagcg tcgtcgcggc gtaatgctgt aacttcgctt tatcgcccac
    21241 cagcggcagc agtgtttcac tgcgggtaag cgtggcgtaa ttctcaacaa tgttggcggt
    21301 gttgacggtc agttcgaagt gccactgtag cgcttcgttc agaggcaacg ttttgccctc
    21361 gacagtgaca ggttcatcgc ctgtcagcca cagcagttcg acaagttctt tcaccagtgc
    21421 cggatcgttc tgataccaga cgcccagcgc gtcacccggc tggtaacgca ggcccgagtc
    21481 gcctaagtca atttcgatat ggcgaacgtc tttttcagag ttacgcccag taattttctg
    21541 gttaacagag aggctagcca ccagcggcgc gtctttgctg tacgggctgg tgtggatttc
    21601 atttaccgcg ccagtagcga cggattgcga aggtgccgcg acaggcgcac gcgatttaag
    21661 cgcatcaacc acgcgggcgc gccactcgct ggcagcagcc tggtattcaa catcagaatc
    21721 gacacggtcg agcaggcgtt caccacccag ttccgccagc ttgctgtcaa aatctttccc
    21781 ggactggcag aaaaattcat aagagctatc gccgaggcta aacacggcaa acgcggtgtt
    21841 ttccagcttt ggcgcttttt tggagaacag gaacttatgc agcgcgacgg cttcttccgg
    21901 cggttcccct tccccttgcg ttgacgtcac tacgatgagc agtttttcgc tggcgatttg
    21961 tttgaattta tagtcgcccg cgttcaccag cttaacgttc agttttgctg ctaataaatc
    22021 atcgcgtaat gcttcagcaa cccggcgcgc attgccggtt tgcgaagcag agataatcgt
    22081 tatacccggc atttctgcgg cgggcgctgg cgtcactgca agcgcaacag gctgctggtt
    22141 gagcacgccc cagaaatagc cagaaaccca ggcaagctgg gtaggcgtta aatcggtcgt
    22201 ggccgcctga aggcgtgcca gttgctccgg gttcaacgga agcaacgcgg aaggtgggac
    22261 ctgtgtcgtc atgcgtcgtt atgttccagt aagcaaagct gtttctgcgc cctgtcagcg
    22321 cccataaaac agaagagaag gtaaggttaa cggggtgaac ggtgtggatt aaagacggga
    22381 tagcgataac taataaccaa atcgactaac ctgttttagc aatagtcttt aacaacaaaa
    22441 tagattaacc aacctaatga aaaacaaatg aatttagcca atcattaaga taaatcagcg
    22501 attttgcgca acaaagtcgt tttagataat gcgaaaaaac agcctttccg gtactctacg
    22561 gcggttttat cgtcctgtag agaaattatg atgtccacca cgttatttaa agatttcacc
    22621 ttcgaagccg ctcaccgctt accacatgtc ccggaagggc ataaatgtgg tcgcctgcac
    22681 gggcattcct ttatggtgcg actggaaatt accggggaag tcgatccgca tacgggctgg
    22741 attatcgatt tcgctgaact gaaagcggcg tttaagccaa cttatgagcg cctagatcac
    22801 cattatctca atgatattcc aggtctggaa aacccaacca gcgaagtttt agcaaaatgg
    22861 atttgggatc aggttaaacc cgttgtgccg ctgttaagtg cggtgatggt aaaagaaacc
    22921 tgcaccgcag ggtgtatcta tcgcggcgaa tgataagagt gtgtcggcgg tcaatttccc
    22981 tttagtaacg ctatgttagg gtgttgtgtt ctggatatct ggggcatgac atggaagacg
    23041 actgcgacat tattattatt ggtgccggta ttgcaggcac cgcttgcgcg ttacgctgcg
    23101 cgcgagcgga tttatccgtt ttgttactcg aacgcgctga aatccccggc agcaaaaatc
    23161 tttccggcag gcggttatat acccatgcac tcgcggaact cctcccgcaa tttcatctga
    23221 ccgcgcctct tgaacgacgc atcactcacg aaagcctttc cctgttaacg ccggatggcg
    23281 caacgacgtt ttccagctta cagcccggcg gtgaatcctg gagtgtatta cgtgcacggt
    23341 tcgatccgtg gctggttgcc gaagccgaaa aagaaggtgt cgaatgcatc cccggagcga
    23401 cggtggatgc actgtatgaa gaaaacggca gagtctgtgg cgttatttgt ggtgacgata
    23461 ttctccgcgc ccgttatgtg gtgctggcag aaggtgccaa cagcgtcctg gctgaacgtc
    23521 acgggttagt gactcgtcct gctggcgaag cgatggcgtt ggggatcaaa gaagtgctgt
    23581 cgctggaaac atccgctatt gaagaacgtt ttcatctgga gaataacgaa ggcgcagcgt
    23641 tgctgttcag cggcggaatc tgtgatgact tacccggcgg cgcatttctt tatactaatc
    23701 aacaaacgct ctcgttaggg attgtttgcc cgctctcttc ccttacgcaa agtcgtgttc
    23761 cggcaagcga gctgctgact cgctttaaag cgcatccggc agtgcgcacg cttatcaaaa
    23821 acacggaatc actggagtat ggtgcgcatc tggtgccaga aggtggcttg cacagtatgc
    23881 cggtgcaata cgccggtaac ggctggctgc tggtgggcga tgcgttgcgc agttgcgtca
    23941 ataccggaat ttccgtgcgc ggcatggata tggcgctgac tggcgcacag gcggcggcac
    24001 aaacgctaat aagcgcctgc cagcaccgcg agccgcaaaa tctgtttgcg ctttatcatc
    24061 acaacgtaga gcgcagcctg ctatgggatg ttctacaacg ttatcagcat gttccggcgc
    24121 ttttgcaacg ccctggctgg tatcgggcgt ggcctgcgtt aatgcaggat atttcccgcg
    24181 atttatggga tcagggtgat aaacctgttc caccgctgcg ccagttattc tggcgtcatt
    24241 tacgtcgtca tggcctgtgg catctggcgg gcgatgttat caggagtctg cgatgtctgt
    24301 agcccgtaat ctctggcgcg ttgctgatgc gccgcacatt gttccggctg actccgttga
    24361 gcgtcagatg gtacaacggt tgattaacgc ctgtccggta ggtctttttt ctctcacaca
    24421 ggaaggtggc ttacgtattg actatcgcgg ctgcctggag tgtggcacct gccgtttgct
    24481 gtgcgacgaa tccacactac aacagtggcg ctatccgcct tccggattcg gcatcaccta
    24541 ccgctttgga taaataataa ggataattta tgcccctctt acacctgctc cgccagaatc
    24601 cggtgattgc tgccgttaaa gataatgcca gcctgcaact ggccatcgat tctgaatgcc
    24661 agtttatttc cgtgttgtac ggcaatatct gcaccatcag taatatcgtt aaaaagatta
    24721 agaacgccgg gaaatatgct tttattcatg ttgatttgct ggaaggcgcg tcaaataaag
    24781 aagtggtgat tcagtttttg aaactggtga ccgaggcgga tggaattatc agcaccaaag
    24841 catcgatgct aaaagcggca agagcggaag tttttttctg tattcatcgc ctgtttattg
    24901 ttgattcgat ttcgtttcac aacattgata agcaagttgc gcaatcgaat ccggattgta
    24961 ttgagatcct gccaggctgc atgcccaaag tgctgggctg ggtgacggaa aaaatccgcc
    25021 aaccgctgat tgctggcggg ctggtatgcg atgaagaaga tgcgcgtaat gcgattaacg
    25081 cgggtgtcgt ggcgctttcc accacgaata ccggagtctg gacgttagcg aaaaaattgc
    25141 tttgacggga taatcgtaac caattgaatt tgttttgatt tgttggccgc acgccacatc
    25201 cgacattcag cgcctgatgc gacgcttaac gcgtcttatc aggcctacag gtccccagtc
    25261 cagaaccgta ggcaggatca tccggcagca cgttattggt attctgcgtg aatgtttgtc
    25321 accagtgcct ccagcaccac tttccagtca tcaaccacgt caatatctgc ctgtgaaaat
    25381 accgctgcgc tggcgtcatg attaatcgcc accataaact tgctattgcg tacgcccgcc
    25441 attaacgctg ctgcgccgga agcgccaaca acaatgcaca cctcaggtgc cagcagatgt
    25501 ccggaaatac caatcacttt ttcagcatca acgccaccgt tcatcactcg cgcccgactg
    25561 tagcccactt cggttcccag cttttctgcc agcataacga tttcctggtt atctgcctct
    25621 ccgccctgcc ccaccaccag cacccgtctg gcctcagcta atgggtcgcg gctaacattt
    25681 ttcagatcct ctgtgctaac cagccagtcg ggaagcgcac ctgggacaat gttcagttgc
    25741 tgcattccag agggcaacgt ggcgtttttc gctgctcccg cctgacgcgc cagggaaagg
    25801 cataacgggc gtttttctgt ttgcagcgtc gccgttaacg catttcccca gtgggatttg
    25861 cgcatgctta cggttggaat atctaacgaa gtcacctgac agatgcttgc accatgtaaa
    25921 cgccaggcca gccgggtcga cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg
    25981 tccgcaggcg ttcgctgcca ctgttccacc atcgcatcca gcacctgttc cgccactacg
    26041 ggctgaggtt ctatttgcca gtgcgccagc gtacaaccgc tgaaatcctg cgccgccagc
    26101 cagctcgcta ttgcggcatt ctcctgattt attgtcacaa ttgcgatatt catggctgca
    26161 tcctttggcg taaatagtcc tgccatagct tttgagcttt ttctgctacc gtttgcccat
    26221 caatcagcgt cgctccgcat cgttgctctg ctcttgccag ttgcagacac tgcatcgctg
    26281 gcatttcggc agcaacagtt ttacggataa tttctgcttt ccccgcagcc attcgctggc
    26341 gcattcccgg tactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca
    26401 agcgaacccg gcaacagcgc aacccatgtt cagtacgctg ttcgagggtg ataaacagcg
    26461 cgtcgagagt gaaacgctcc accagggtga agcagggcca gcccagcatt tccgccagca
    26521 aaaatggcgt ttgcccattc tgcccttcgc tgctttggca gccggtaatt atcagatcca
    26581 gcggattctg acgctgccat tcggcaatat ggcgagcgac aaattccggc gcaaagcgca
    26641 gatctgccgc cgtctccagc aatacagctt cctcaaaccc aagagccatg agatagcgca
    26701 gccagtgcaa cgcccgttca tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt
    26761 tttttctctg cgctaacagc agcgcggcag cggcctgttc atcagcaccg agtaaacttc
    26821 gcagtagcga aacatccggt ccgctattac cctgagccgc cgcctgccac tctttttccg
    26881 ccagcattcc ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcct
    26941 acatttcccg ccgccaccag cgggagggct ttagtttctg gtgcccatag ccaggtcaca
    27001 acaaaaccaa ccaacaacac tgtcgccagg agcaataagg tgacttgcat tccccactgc
    27061 gccagcaccc acggcagcag gccagtactt accgccgcgc caagtcgact catggcggtg
    27121 gcaaaaccga cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc
    27181 accagattac tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat
    27241 gaactgccgg aaggcaaaca ggccattact accagcgttg ccgccagcag caaaaaactt
    27301 cccagcaaaa atttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcgccc
    27361 acaattaaca acgcattaag catcaggctg gcagtcagcg catcttccag accaatagtc
    27421 tgggcgatag ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag
    27481 aagatgctgt taaacgccgt gcgccgccag taacgcgaag agaacaaggt tttgatgtgt
    27541 ttatgggtcg ccgttaccac ttcatcgccc agtaaaacat ggggaccaaa atagcgatgc
    27601 acgatagcgt gagcttctgc aaaacgcccc tggcacagta gccaacgtgg cgattctggc
    27661 gttccccagc gtaataacgt aatcaacaac gcgggcagag ctgccgatgc cagcaaccag
    27721 cgccaggcct ccgggttttc ggaaataaag tgatgtccgg caatacttgc caacacatag
    27781 cctacggtcc acaccacgct gaatgcgccc agcaaaatac cgcgatggcg gcgcggggaa
    27841 aattcagcca gcaaggtgtg acctactgag taatcgcctc ccagaccaat gccgatcaaa
    27901 atgcgcagcc caataagatg ctctggcgtg gtggcaaaaa attgcaagaa cgaagcgagc
    27961 gtaatcagca aaaagctgaa ggtgaagatt ttttgccgac caatatggtc ggagatccac
    28021 ccaagaacca ggctgccaag aaacaaacca aggagcgccg agccgccgct cattcccgcc
    28081 ataaacggcg tcagttgcat ggcgggcgta agttgaataa tggcgtaacc aatgacgccg
    28141 agaacataac cgtcggtcag gtgtgcgccg aaagtgagcg cagcaattcg acagtgaaaa
    28201 cggttaagcg gtaaatcatc cattcgcacc ggtgaagtgt tcatctttac cccttcattg
    28261 tccggtaata agaacggctt ctcgcctgag aagccgaaaa ggcaacgccc cgtcatcgcg
    28321 gaacgctgcc tgatacatta tttttcaatc ggatagatag tacccgtatt cataatgccg
    28381 ttaggatcga actgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagc
    28441 ttgctccagt gaacgcgatg tttaccaatg ccatggtggt gcaccatcga accgccgagg
    28501 cgaatggttt cttcgcagat gattttgttg agcggattgt ggtacttgtc gatttcctct
    28561 tccggcttac agtcaacaac gttgtagtca tagacgaagt acatgttggt gccgttctga
    28621 tagctatgag aggagtgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcagta
    28681 cgaatacggt taataacgct ttcgtagatt tcgtggatgc agctccagca gccggacact
    28741 tcggtggtaa agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta
    28801 tccggtcccc agttcaggtt gttaaaccag gtttcaatca gtttgctgtc cacgcgctgg
    28861 cactgcgggt agcgggcaac gatttccgca atcccttcgc ccgtcgcctt cgcaatgcga
    28921 gggttacctt cagccataaa gatcagcacc cattttccgt cggcaaaatg ggtgaagtgt
    28981 tgggtgccat cttcagcgtc atacaaacga gcgatcgacg gacgataccc ttccaccatg
    29041 atttcacgca ggatgttgaa gccggttttc atgtcttcca ggatatagcc gtagaagagg
    29101 ttgttttccg gggtgtattt aaagattttc actgttactt cagtgatata gcacaatgca
    29161 ccttcgttgc cgatgatgat gtgacgaatg tccgggccag ccgcgcggcg tggcacgttt
    29221 ttaatgcgtg tgacggtgcc atctgccaac actgcctcca gaccaacgac catatcttcg
    29281 attgcgccgt agagtgtgga gaactgcccg atactgcggg ttgctaccag gccgcccatc
    29341 tgcgccagcg gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgcg
    29401 ttttccagca cttccagcgg aacaccacat tgcgccgtcg cctgcatatt ctcaatatca
    29461 atattaatga tttgattcat ggcggagccg tcgagcacca ccgagttttc tacaacagtt
    29521 tccagcccac cttcggtggc ggaagcaccg gtacgcggca caccgttaat tttgtgcgca
    29581 ttcataaaat tcagcacacg ggatacttac tctgtggaac cgagttttac gaccgctgcc
    29641 ggaatcggca aagtataaat gccatgaata tccggaaatt tacgaaaacg gtcaatactg
    29701 tttttcttta atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagc
    29761 tggtcgacaa tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg
    29821 aataatcatt aataagagaa aacagaaacc accgttatgg attagcgcac taaataaccg
    29881 ccatcgacca ctaataaatg cccgttgaca taattcgatg cctgacttgc gagaaacacg
    29941 gctgcgccca ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg
    30001 cgctgattgg tttctggatt actgcgtgtc gccagcataa tatgctgttg cataatagcc
    30061 aggggcgata ccatttacct gaatattata ttgacctagt tcatcacaat aagctttggt
    30121 gaacccggca agagcatgtt tagtggcaga atatgcaggt gaccattgtc cacctaagta
    30181 agagaacaat gaacagatat taataatttt gccgcttttc tgcgggatca taatttttgc
    30241 cgcttcatag cttaactcga atgcggcggt caggttcaca tcaatcatcg gatcccagtc
    30301 ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt taaccagaat
    30361 atcgacagta ccgaagcgct cacagcaggc agcgataatc ttctgcggtg cgccttctgc
    30421 ggtaatatcc acctgcatga agtccacttc aacaccctgt ttttcaatca tttcctttgt
    30481 ttcgccgtta tctttgacga agctaggaat aaaaatattt gcgccagctt tggccaacgc
    30541 cattgcaaat gcctgaccta aaccgctatt cccgccggta acaattgctg ttttaccttt
    30601 cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
    30661 tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
    30721 cattatctct ggtaactgga gataattttt agcataacgg cataactgct aatgtgatcg
    30781 taatcacagt gtgatattcg tattgtcaaa tagttttaaa aagcagaagc gtgattactc
    30841 tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa
    30901 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
    30961 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
    31021 ggcggcgtta gttttgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
    31081 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggct
    31141 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
    31201 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
    31261 tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat gctgtggtcg
    31321 gtgtcgatta aagccgcctc gttgcttggc gatcatagcg agcaagggaa aattatgggc
    31381 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
    31441 ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cagtcattat catctacagt
    31501 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
    31561 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct tgccgttttg
    31621 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gtgtcttcac aatctacgcc
    31681 attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct ggtggcagcg
    31741 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggaggc
    31801 attatcacca cctacagtaa agtgaaatcc cctacccgcg tgattcaaat cctttccatc
    31861 atcggtttgc tggcgttgac agccctgctc gtcacgaact ctaacccgca atcggtcgcg
    31921 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
    31981 tgggcctgcc ccggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
    32041 tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg ccactggcaa
    32101 gacaccctgc ccgctgcaga agcctaccgc aatatgtggc tgatgggcat ggcggcgctt
    32161 gccatggtga tagtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
    32221 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
    32281 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgata tacgatctgg
    32341 aaggtaatgt ggtttgcgaa gggaaaggct tattgcagcc gatgcacacg ccagatgccg
    32401 atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc ggtcacgatt
    32461 tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg ggatccatcc
    32521 gttgctgccg tgcgttattg aaagccgatg gcacggccgt tgattagctg gcaggatgca
    32581 cgcgtaacgc gtccctacga acacactaat cctgacgtgg catatgtcac ctctttttcg
    32641 ggttatctga cgcatcgctt aaccggcgag tttaaagaca atatcgccaa ctattttggt
    32701 cagtggccgg tggattataa gagctgggca tggagcgaag atgctgcggt aatggagaag
    32761 tttaatatcc cccgtcatat actgtttgat gtgcaaatgc caggcactgt tcttgggcat
    32821 atcacaccac aagccgcgct ggcgacacat ttcccagctg ggctgccggt tgtttgtacc
    32881 accagtgata aaccggtaga agctctgggg gccggattac tggatgatga aacggcggcg
    32941 atttcactcg gtacttatat cgcgctgatg atgaacggca aagccttgcc gaaagatccg
    33001 gtggcgtact ggccgattat gtcttctatt ccgcaaacat tgctgtatga aggttacggt
    33061 attcgcaaag gtatgtggac ggtgagctgg ctgcgcgaca tgttaggcga gtcgttaatt
    33121 caggatgcca gggcgcagga tctttcaccg gaagatttac tcaacaagaa agcctctagt
    33181 gtgccacctg gctgtaatgg gctgatgacg gtgctggatt ggctgaccaa tccgtgggaa
    33241 ccgtacaaac gcgggattat gatcggcttt gattccagca tggattacgc atggatatat
    33301 cgttcgatac tggaaagcgt ggcgctgacg ctgaagaaca attacgacaa tatgtgtaat
    33361 gaaatgaatc actttgcgaa gcatgtgatc attactggcg gcggttcgaa cagcgatctg
    33421 tttatgcaga tttttgccga cgtgttcaac cttccggcac gacgtaacgc cattaacggt
    33481 tgtgcaagcc tgggggcagc gattaataca gcggtaggtc tggggctata cccggattac
    33541 gcaacggctg tcgacaaaat ggttcgcgtg aaagatatct ttatgcctgt tgagagcaat
    33601 gccaaacgct acgacgcgat gaataaaggt attttcaaag acctaaccaa acatactgat
    33661 gtgatcctga aaaaatcgta tgaagtgatg catggggaat tggggaatgc ggattcgatc
    33721 cagagctggt cgaatgcgta agtgaggaag accgggcggg aaactgcccg gcctgaatat
    33781 acctgaatgg ttatccccgc tgacgcgggg aactcgaata actgaccaac gtctggctct
    33841 ctaaatccgg tttatccccg ctggcgcggg gaacactcta aacataacct attattaatt
    33901 aatgattttt taagccagtc acaatctacc aactttacag gatcacacaa acaacacatc
    33961 cattatgtta aagagcactt aatccattga ttaaa
//
LOCUS       DAJTRQ010000028        33231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000028 DAJTRQ010000000
VERSION     DAJTRQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            101..325
                     /locus_tag="OO332_001471"
                     /pseudo
     CDS             101..325
                     /locus_tag="OO332_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
     gene            499..1268
                     /locus_tag="OO332_001472"
                     /pseudo
     CDS             499..1268
                     /locus_tag="OO332_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1301..2821)
                     /locus_tag="OO332_001473"
     CDS             complement(1301..2821)
                     /locus_tag="OO332_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5941999.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SLFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2912..3631
                     /gene="tonB"
                     /locus_tag="OO332_001474"
     CDS             2912..3631
                     /gene="tonB"
                     /locus_tag="OO332_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5942000.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3671..4069)
                     /gene="yciA"
                     /locus_tag="OO332_001475"
     CDS             complement(3671..4069)
                     /gene="yciA"
                     /locus_tag="OO332_001475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5942001.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4174..4713)
                     /locus_tag="OO332_001476"
     CDS             complement(4174..4713)
                     /locus_tag="OO332_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5942002.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4743..5486)
                     /locus_tag="OO332_001477"
     CDS             complement(4743..5486)
                     /locus_tag="OO332_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5942003.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5514..5603)
                     /locus_tag="OO332_001478"
                     /pseudo
     CDS             complement(5514..5603)
                     /locus_tag="OO332_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5843..6481
                     /gene="ompW"
                     /locus_tag="OO332_001479"
     CDS             5843..6481
                     /gene="ompW"
                     /locus_tag="OO332_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5942004.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6541..7047)
                     /locus_tag="OO332_001480"
     CDS             complement(6541..7047)
                     /locus_tag="OO332_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5942005.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(7093..7593)
                     /locus_tag="OO332_001481"
     CDS             complement(7093..7593)
                     /locus_tag="OO332_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5942006.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7679..7858)
                     /locus_tag="OO332_001482"
     CDS             complement(7679..7858)
                     /locus_tag="OO332_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5942007.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8239..9045)
                     /gene="trpA"
                     /locus_tag="OO332_001483"
     CDS             complement(8239..9045)
                     /gene="trpA"
                     /locus_tag="OO332_001483"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5942008.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(9045..10238)
                     /gene="trpB"
                     /locus_tag="OO332_001484"
     CDS             complement(9045..10238)
                     /gene="trpB"
                     /locus_tag="OO332_001484"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5942009.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10250..11611)
                     /gene="trpCF"
                     /locus_tag="OO332_001485"
     CDS             complement(10250..11611)
                     /gene="trpCF"
                     /locus_tag="OO332_001485"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5942010.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(11612..13207)
                     /gene="trpD"
                     /locus_tag="OO332_001486"
     CDS             complement(11612..13207)
                     /gene="trpD"
                     /locus_tag="OO332_001486"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5942011.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13207..14769)
                     /gene="trpE"
                     /locus_tag="OO332_001487"
     CDS             complement(13207..14769)
                     /gene="trpE"
                     /locus_tag="OO332_001487"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5942012.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14791..14883)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            15042..15923
                     /gene="yciV"
                     /locus_tag="OO332_001488"
     CDS             15042..15923
                     /gene="yciV"
                     /locus_tag="OO332_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5942013.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15920..16540
                     /locus_tag="OO332_001489"
     CDS             15920..16540
                     /locus_tag="OO332_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5942014.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16641..17513
                     /gene="rluB"
                     /locus_tag="OO332_001490"
     CDS             16641..17513
                     /gene="rluB"
                     /locus_tag="OO332_001490"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5942015.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17553..18143)
                     /gene="cobO"
                     /locus_tag="OO332_001491"
     CDS             complement(17553..18143)
                     /gene="cobO"
                     /locus_tag="OO332_001491"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5942016.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18140..18898)
                     /locus_tag="OO332_001492"
     CDS             complement(18140..18898)
                     /locus_tag="OO332_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5942017.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19118..20167
                     /gene="sohB"
                     /locus_tag="OO332_001493"
     CDS             19118..20167
                     /gene="sohB"
                     /locus_tag="OO332_001493"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5942018.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20203..20454)
                     /locus_tag="OO332_001494"
     CDS             complement(20203..20454)
                     /locus_tag="OO332_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5942019.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20834..23431
                     /gene="topA"
                     /locus_tag="OO332_001495"
     CDS             20834..23431
                     /gene="topA"
                     /locus_tag="OO332_001495"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5942020.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23641..24615
                     /gene="cysB"
                     /locus_tag="OO332_001496"
     CDS             23641..24615
                     /gene="cysB"
                     /locus_tag="OO332_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5942021.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24910..25074
                     /locus_tag="OO332_001497"
     CDS             24910..25074
                     /locus_tag="OO332_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5942022.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            25077..25244
                     /locus_tag="OO332_001498"
     CDS             25077..25244
                     /locus_tag="OO332_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942023.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25358..25453
                     /locus_tag="OO332_001499"
     CDS             25358..25453
                     /locus_tag="OO332_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942024.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25617..28292
                     /gene="acnA"
                     /locus_tag="OO332_001500"
     CDS             25617..28292
                     /gene="acnA"
                     /locus_tag="OO332_001500"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5942025.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28356..28946)
                     /gene="ribA"
                     /locus_tag="OO332_001501"
     CDS             complement(28356..28946)
                     /gene="ribA"
                     /locus_tag="OO332_001501"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5942026.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29116..29880
                     /gene="pgpB"
                     /locus_tag="OO332_001502"
     CDS             29116..29880
                     /gene="pgpB"
                     /locus_tag="OO332_001502"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5942027.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            30029..30337
                     /gene="lapA"
                     /locus_tag="OO332_001503"
     CDS             30029..30337
                     /gene="lapA"
                     /locus_tag="OO332_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5942028.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30344..31513
                     /gene="lapB"
                     /locus_tag="OO332_001504"
     CDS             30344..31513
                     /gene="lapB"
                     /locus_tag="OO332_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5942029.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31706..32443
                     /gene="pyrF"
                     /locus_tag="OO332_001505"
     CDS             31706..32443
                     /gene="pyrF"
                     /locus_tag="OO332_001505"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5942030.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32443..32769
                     /gene="yciH"
                     /locus_tag="OO332_001506"
     CDS             32443..32769
                     /gene="yciH"
                     /locus_tag="OO332_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5942031.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32895..33113)
                     /gene="osmB"
                     /locus_tag="OO332_001507"
     CDS             complement(32895..33113)
                     /gene="osmB"
                     /locus_tag="OO332_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5942032.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         8432 a         8030 c         8442 g         8327 t
ORIGIN      
        1 taagaccgca cgaatataac ggtgggttac ccccaaacga atcggaaaat cgatactgga
       61 aaaactctaa ctcggtggcc agtttttgtt gaccacttca gttccacagt tttcacctga
      121 agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa aggttatcaa
      181 accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg tacttgatgg
      241 aaaggttatt gatgaaagaa ccacatctca tattaatcgt atgcaggaag ataccttgat
      301 tagctcagct tacccagagg aataaacaat gttagaacta ctaatattta ttgccacaca
      361 tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc gaatgctacg
      421 tgatttcttc tgagcagata ccaacatcaa aaattagatt tcacgtagct cacgcttaac
      481 agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg taaagatggt
      541 atctattttc accttagaga taatgtagaa tcttttcttg ccttaacagc tactgaacag
      601 atggactatg actcaccgat gatggtgaca accaggctat gcaatcgggg caaactatca
      661 ctgataatgt tcagagagca cctaagacca tctctattag cggtgttgta gtggttgatt
      721 cgacgggttc agttctatta accagacagg ggcaacttgt agagaccttt atttctacgg
      781 ttgaacaatg gcgagaccag aagcaaatca tcactattgt ttgtaaagat ggaatatcta
      841 tcactgatag tatcattact cagtttaaag cgagtaaaga gcataccatt gctaatggtc
      901 ttcgtattca gatgaccttc caaggtgtag aatttcgttc aatcgtaggg caaactgata
      961 tttcagcagc tactggcaag accgctacca cgaatgatgg tggtgctacc agtaaaaaga
     1021 acactggcaa cactacaacg agtcttggaa gccctatgct gacctgtaaa gagcttttta
     1081 gctattcggc aagtgagtta tcagatgaag cgctaaaagc ccgtgtaacg tgctctaaga
     1141 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatgag cgagcttcaa
     1201 aagtgcttaa gtcgggtaat gctttacgga agtactctgt gaatccgaat aagaagggca
     1261 cttattgaga cataaaaagg ggcttcatag cccctgaaaa ttacagttga taagcatcaa
     1321 tatccagtag ctctttatca gcaatttcaa agattggtga cccatcattt agcggtagcg
     1381 ggtctgtagg tatggtgatt tcagtgtcgt tctgtagcgt tatagttact gaacccttac
     1441 ccaatgtaag cccttttaga tgtttggaca aaattaattg acactcgaca cgacccgctt
     1501 tagttgtcag gtcaatatct ttaagctggg gtagttcctt atcagtgagt gaagccttac
     1561 gcttcatagt atcaatcgtg gtctgtagtt ctgtgcgctt ctggttaagt tccttaagct
     1621 gaatttttag ttcatcaata tcatccatct caagaagtag ggcggtagct ttcttgattc
     1681 tggctgtgac aatttcaacc tcatgctgta acgctgcaat cttcctctct gattctccat
     1741 cattccccac gtcaatcatc cccagaaaat tatcaatcat ccatcggtca aacggtttac
     1801 gcttcaacgg tggaacatca cagcgaccgt ctaagtggtt attgcacctg tagaccccag
     1861 catacttatt ctttgttccg gtaggatgga cactagcccc acactgacac ctaaacagac
     1921 ctttgaagat gttaatcgtt agtgggttgt cacttgcagg tgtacggcct ttacgattct
     1981 tatcaagaat ctcttgagct ttattgaacg ttgcagcatc tatagcggct tcgtagtagt
     2041 tcggtatagc ggggcggtca ttgcgctgtg atgctggtag tgtacctatc gttgtctctg
     2101 accttaagat agtacgcaca gaatgaacgc taaatccatt agcatcttta cgtcgtgaga
     2161 ataaactaat gttattagcc tgtaggtgac gtgcaatgtt atgcagactt tcaccatcta
     2221 gcagcagctt gaaaatcaag cgtacagttt cagcacgttc attgagttta aaagtctcag
     2281 ggtcaatcca tccgggaaca ttccttgtta aacgttgttc accagcaagg gcaagctttg
     2341 cacgttgttc ccatttctga ctaacacgga aagacttctg tttactctcc tgatgcgcta
     2401 tgtagctttt aacccgctga atgattgaga ttcctaaatc atcggataga gcatcccttg
     2461 tgtagattag cccatcctgt agagtgtgaa tctcgatacc atgagcaata atgcgattca
     2521 tcaaggcttc tgtttcaaga tgttcaaggc ggcctatgcg gtcgatagct tcgactagta
     2581 aaattgtccc ttctggaaac tcccctgatt caatgcggga gagcatatcc ccaaattgcc
     2641 ctgataagtg tttacctttg taaccggata gaccttcatc attgaaagat gccactacct
     2701 gagcatcgct attcttcctt aaccactgtt tgaatagctc attttgtcgt ctggttgaat
     2761 cagcaccttc agcaccttgt gtggctgatg agaatctcaa gtatgttatt acggttctca
     2821 cggtgataaa ctcccatgca attgattatc atttaaagat gaacttataa acgttatggc
     2881 taatgctaca gtgccgaata gctacaatac aatgcccctt gatttacctc gccgcttccc
     2941 ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcgggtc tgctctatac
     3001 ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg tcacgatggt
     3061 tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc cggtggtaga
     3121 gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg tggtcattga
     3181 aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac aggagcagcc
     3241 aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa atacggcacc
     3301 cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta ccagtgtggc
     3361 ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag cacaggcatt
     3421 gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc gcgtggataa
     3481 cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga aaaatgcgat
     3541 gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga atatcctgtt
     3601 taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc cgaccggagg
     3661 cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca accgcgacat
     3721 acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac gctacttttt
     3781 tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca cagcgtgcat
     3841 agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct tcaacccgca
     3901 cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca atatccattt
     3961 gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg ggcatggcta
     4021 aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg gtaaaaccga
     4081 cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga acagaggaga
     4141 tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct gcggcatgtg
     4201 gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa
     4261 gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga tgtttgccag
     4321 accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata ccggttgcgg
     4381 cagcgtgagt tctttaccca gcatccgctg aattagcggc tttttcatca cccattggct
     4441 gactaacagg gctaccgcaa acagagcata aatgactgta accttccatt taataaactc
     4501 atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa aagtgatcag
     4561 ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa tcgccgtggc
     4621 gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac
     4681 aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa
     4741 ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat ggcagaaatc
     4801 aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa
     4861 gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc gggtgccacc
     4921 agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa aatgcccatt
     4981 ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat aattcccgga
     5041 acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat
     5101 aacttgggca atatcggcgc actggcacca atagctcgca gcgcacagac tctctgaccc
     5161 gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt accgattaat
     5221 tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata
     5281 ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc attgagctgc
     5341 gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat
     5401 agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc ggtgtcacgg
     5461 tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta
     5521 gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt atggaaaagc
     5581 atatctttgt aaagcggagg taattgctgg ccttcaggcg tgggcaaacc gcggcaagtg
     5641 gcgatcgagt taatatttgc gtagagaaaa tatttaaaaa ttgatttaaa tcacattaac
     5701 caggattctc aatgcaactt ctaaattaat ccagatcaat aaatggtgaa ttatcatatg
     5761 taatgtgatc tatgtaggat catttgttac tccaatgtag gtatattcgt cacgttttta
     5821 taaccataac gacggagcgg atatgaaaaa gttaacagtg gcggctttgg cagtaacaac
     5881 tcttctttct ggcagtgcct ttgcgcatga agcaggcgaa ttttttatgc gtgcaggttc
     5941 tgcaaccgta cgtccaacag aaggtgctgg tagtacgtta ggaagtctgg gtggattcag
     6001 cgtgaccaat aacactcaac tgggcttgac gtttacttat atggcgaccg acaacattgg
     6061 tgtggaatta ctggcagcga cgccgttccg ccataaaatc ggcacccggg cgaccggcga
     6121 tattgcaacc gttcaccatc tgccgccaac actgatggcg cagtggtatt ttggtgatgc
     6181 cagcagcaaa ttccgtcctt acgttggggc cggtattaac tacaccacct tctttgataa
     6241 tggatttaac gatcatggca aagaggcggg gctttccgat ctcagtctga aagattcctg
     6301 gggagctgcc gggcaggtgg gggttgatta tctgattaac cgtgactggt tggttaacat
     6361 gtcagtgtgg tacatggata tcgataccac cgccaattat aagctaggcg gtgcacagca
     6421 acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat atcgttttta
     6481 attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt ataaagggta
     6541 ttatttcttc gcttctacgc aatcagtttc agaacgaatt aaaaatttct cagttgtttg
     6601 cggaatatgc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa tcgcttcgat
     6661 gatcggaatt gagactgtat caccggcatt ttttgctgct gctaatagtg aggtgtaaca
     6721 ggcgatttca aattgctcga agacatatcc gctaatagag cctttaacta tttcatcaga
     6781 agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg aatctttaat
     6841 gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa tctggttttt
     6901 ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat ctatacggct
     6961 ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt cacgtaacca
     7021 gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg actaaaagtt
     7081 gaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa gggccagatc
     7141 agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa gcttcgctgc
     7201 tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg caatctcata
     7261 atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt ttttctcggt
     7321 actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac atttcatgcg
     7381 cttaattttc agatttgatt ctgattccac aacttgatca attcgttcaa tctgtccatg
     7441 agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg ttgctcttgc
     7501 gagttttgcc agtgcccggg gtaattgttt ttctgcgctg taggtatctg aaagcagttg
     7561 aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa tcactgcgag
     7621 ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta acggtgaatc
     7681 aggatttgtc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc gcttcagatg
     7741 cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta cggcctgcgt
     7801 cggatgcctt ctcacggtct tcggcgaaat ttcctgaacc accacgatgt tcggccatgt
     7861 tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc gacactcatt
     7921 aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata gagggaaaat
     7981 atattttgag gaacattctg gatatattaa caattacctg aggaataaat gacttagaga
     8041 aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta tattactgtt
     8101 gggcggaaaa atgacgtaag ttgacgttcg accggggtaa gcgaaacggt aaaaagataa
     8161 atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat gccgccagcg
     8221 gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta caaaaacttt
     8281 cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt taacaatggc
     8341 cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct gatccggggc
     8401 ggaaatacca aacccctgca atggaggtgc agcgttgtac tctttcagct tcgcaaccag
     8461 atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcccgctc gtgacagcaa
     8521 atatgtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat cggcatttgg
     8581 cgggcagatg aagataggtg cgacattatg gcgcaacgcg gcctggcgga agggcgcgga
     8641 ctcttcaact ggcacatcgg caaccagcac cgaatcgacg ccgactttct cgcactgggc
     8701 ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca ggccaatggg
     8761 aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg ccggagtcac
     8821 acctgccgca aaggcgcgca gagtggcgtt ttgaatggtc gggccatccg ccagtgggtc
     8881 ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg tatcgataat
     8941 tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaatga atgcgccttc
     9001 tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat ttcccctcgt
     9061 gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc
     9121 accagtagct gctctttatc cgggttttcg cgcatcattt tcagcgcatg ggccagggcg
     9181 tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt gaaggcttca
     9241 agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt aagatacgcg
     9301 tgttgtgggc cgacagacgg gaaatccagt ccggcggaga tggagtaaga ttcttcaatc
     9361 tgcccgtctt cggtttgcat catcggcgct ttcataccga aataaatgcc cacgcgacca
     9421 tgttttagcg gtgcgccgtg ctcgccagtt tcgataccgt gaccaccagg ctccacacca
     9481 atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc attcgaaccg
     9541 ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag aatctgcgct
     9601 ttggtttctt cgccaatcat ccgctgaaac tcacggacaa tggtcggata aggatgcggg
     9661 ccagccgcgg tgcccagcat atagtgcgcg gtttcataac tgccggacca gtcgcgtagc
     9721 gcctcattac aggcatcttt cagcgtggcg gaaccgctat gtaccgggat cacttccgca
     9781 cccattaagc gcatacgaaa aacgttaggc gactggcgtt caacgtcttt ggcacccata
     9841 taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac gccatgctga
     9901 ccggccccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag caacgcctgc
     9961 cccagcacct ggttggtttt atgtgcgccg ccgtgcagca aatcttcacg cttgagatac
    10021 agcgtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagtgcggt tggacgcccg
    10081 gcatagtttt tcagcaggtc gttgaactgg gcttgaaatt caggatcttt ttgcgcgctg
    10141 acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac gtacatgccg
    10201 ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct taatatgcgc
    10261 gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcccggt tgcgactcta
    10321 cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg cagttatctg
    10381 cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat agtgaccagt
    10441 caaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca acgtgctgaa
    10501 actcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca acatgtgctg
    10561 gcagagcttc acgcagcgta tcgatataca gctgatcttc attaccatgc agttgcactg
    10621 ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga ttgcggaaca
    10681 cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca acgttgacgc
    10741 aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg
    10801 ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc aacacccggc
    10861 gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga aaaccgttag
    10921 cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg ctgattaccg
    10981 ttacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca atcgacaaat
    11041 cacgcagatc gcggttgttg atgccaacga cctttgcccc taatgcaatg gcgcgctcca
    11101 gttcctcttc attactgact tcggtcagca cccccatctc cagactgtga gcgacggctg
    11161 caagctggcg atattggtcg tcatccagta ctgaaagcat taataagcag gcatcggcct
    11221 ggtaatagcg cgccagatag atctggtaag ggtcgataat gaagtcttta cataaaatcg
    11281 gctgcggggc gatttggctg acgatgagga ggaaatcaaa gctcccctga aaatatttct
    11341 catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca atgcgtgccg
    11401 gatcgaaatc atcacggatc acgccttttg acggcgacgc ttttttacac tccagaataa
    11461 acgccgtgcg tgcaccttgt agcgcatcat aaaaatgtcg tgtgctcggc tgaacctcat
    11521 tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc gccttgtctg
    11581 cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagc gcggtaactc
    11641 tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg gcttgcagat
    11701 cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct gcttcatggg
    11761 cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct tcaggtgttc
    11821 cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg gtcaattgat
    11881 agctcttaat tttgccgtca tgcagctcgg caacgattgt cggcgcgtgt aatgaaactt
    11941 catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg cgcaaggttt
    12001 cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc ggcggatgcg
    12061 ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt tgctggcgaa
    12121 ccggcatcgc gtggcggaat ccggtgtgat atttcggcgc aaagaggaaa catacgccta
    12181 actcgtccag cgcctggcgc gatttattgg cgttcatatc aagattaata ccgaatgccg
    12241 ccagcagatc cgacgagccg gatttactgg agacgctgcg gttaccgtgt ttcgccactt
    12301 tcagcccaca ggccgcggcg acaaacgcac tggcggtaga aatattgata ctgttgctgc
    12361 cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg aacggcgctg
    12421 cgttttccag tagcgcggtt gctgccccgg cgatctcgtt cgggtgctca ccacgaattt
    12481 tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc accaccgctg
    12541 aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt ttttccagaa
    12601 tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc gtttgttcca
    12661 gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat ccacaaacgc
    12721 gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg atggttaaac
    12781 cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc gggtttgtta
    12841 atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg tgaagaattt
    12901 cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt ccgaggcaaa
    12961 tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata caaccggctt
    13021 cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc gtcgccaggc
    13081 gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacgtta tgaccattgc
    13141 tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc agcagaatgt
    13201 cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg
    13261 ggctttgtta cgggtttcgt cggcttccga ctgcggaaca gaatcaagga ctacaccagc
    13321 acccgcttgc acggtggcga taccgttttc caccagcgcc gagcggatca caatgcaggt
    13381 gtcgagatcg ccatgcgcgg tgaaataacc taccgcgccg ccgtagctgc cgcggcgacg
    13441 accttccgcc tcggcaatta actgcatagc gcgtactttc ggcgcaccgc ttaacgtccc
    13501 catattcata caggcgcgat aagcgtgcag ggcgtcaaga tcgtgacgca gttcgccgac
    13561 tacgcgagag acgaggtgca tcacatagga ataacggtca actttggtga gatcggcgac
    13621 gtagcggctg ccaggggtgc aaatgcgtgc cagatcatta cgggcgagat caaccagcat
    13681 cagatgttca gacagctctt tatggtcagt acgcatttcc agttcaatac ggctgtcgag
    13741 atctctgtcc agtgaaccat cggcgcgacg accgcgtggg cgtgttccgg caatcgggta
    13801 gatctcaatc tggcggctgg tggcatcata cttgagcgag ctttccggcg acgcgccaaa
    13861 tagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac tctttttcag
    13921 cacgtaatag gccgccagcg gtgacgggca gggcagagag aaacggcgag atggcaccac
    13981 ctggaaaatt tctccagcgc gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc
    14041 ttcatcgctc tgattacatt cacaacgcat atgcggcacg gaaaccactg gcagcggcgg
    14101 cgcggcttcg gtcagttgct gacgtagttc gttcaggcga gcagtgagac gttgtttttc
    14161 ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct gatggtcaat
    14221 caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt ccgctgacag
    14281 ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc cgaagaacat
    14341 tgcttctcgt tcttccttcg gtacattcaa cagattctgc aataaacgga aagcgtcaaa
    14401 aaccgaaagg gagcataagc gggcgtcttc atccagcagt ggactgacag gggggaagcg
    14461 cagcacacgg cagtttggtg attgttcact ttccacaccc gcaggcaggg cgttatccag
    14521 tagtgccagc agggcttcgc cattgccgga aagtgcctga attgtgacgg tgtcacctaa
    14581 tgctgtaatg tgcagcgcat tgtctatcag cagcaggctt tttaaatcat ctttgctgtc
    14641 gatatctgcg gattccagca gcagcgttgc cggacgattc ccacacaact ggtgaaaaag
    14701 cgcagtcggg ttgtcgcgat aagcgccttc gcaggttagc agttcgagag tcggtttttg
    14761 tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg
    14821 gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg gccaccaacc
    14881 tttcagtacg aaaattgctt tcattgtcga taccattttt acgtgaactt gcgtactagt
    14941 caactggttc gatgattaat tgtcaacagc tcatttcaga atatttgcca gacccgttat
    15001 gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg
    15061 cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag
    15121 cattggtgca ccgtgcagtc gagatgcgcg tcggcaccct ggcgatcacc gaccatgaca
    15181 ccacagctgc aatcgcgcca gcaagagagg aaatttcacg ttcgggactg gcgctgaatc
    15241 ttattcccgg cgtggaaatt tccacggtct gggaaaatca tgaaattcat attgtcgggc
    15301 tgaatattga tattactcat ccgctaatgt gtgagttcct cgcgcagcag acagaacggc
    15361 gcaatcagcg ggcgcagcaa attgccgaaa gacttgaaaa agcgcaaata cctggggcgc
    15421 tggaaggcgc acaacgactg gcgcagggcg gcgcagtgac gcgcggtcat tttgcgcgtt
    15481 tccttgtgga gtgcggcaaa gccagttcaa tggcagatgt ctttaaaaag tatctggcgc
    15541 gcgggaaaac cggatacgtt ccgccacagt ggtgtacaat agaacaagct attgatgtca
    15601 ttcatcattc tggcggtaag gcggtgctag ctcatcctgg gcggtacaat cttagtgcta
    15661 aatggctgaa aagattggtt gcgcattttg ccgaacacca cggcgacgcg atggaagtcg
    15721 cgcagtgcca gcaatcgccc aatgaacgca cccagctggc cacacttgcg cgtcagcatc
    15781 atttatgggc atcacaagga tctgattttc atcagccatg cccgtggatc gaactgggtc
    15841 gtaaactctg gctgcccgca ggcgttgaag gcgtctggca gctatgggaa cagccgcaga
    15901 acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata acccgcagca
    15961 acgtctgatc aaccaggcgg tggagatcgt gcgtaaaggc ggggtgattg tctatccaac
    16021 tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgccatgg agcgtatttg
    16081 tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga
    16141 actctcgacc tattcattcg tggataacgt tgcgtttcgt ttgatgaaaa acaacacgcc
    16201 gggcaactat accttcatcc tgaaggggac gaaggaagtg ccacgccgtc tgttgcagga
    16261 gaaacgcaaa accatcggta tgcgtgtgcc ttctaacccg attgcgcagg cgttgcttga
    16321 agcactgggc gaaccgatgc tttccacttc gctaatgctg ccaggcagcg aatttaccga
    16381 atccgatccc gaagaaatta aagaccgtct ggaaaaacag gtagatttga ttattcacgg
    16441 cggctatctc gggcagaaac caacaacggt tattgatctt accgatgata cgccggtcgt
    16501 ggtgcgtgaa ggcgtaggtg atgtgaagcc tttcttataa gtgcgtaact ctgtatacta
    16561 cgcggcctta aaatggcgct atacggcgcc attgattctc ctgttcgacg cctgtgaagg
    16621 cgacaccaaa aggaagctct atgagcgaaa aattacagaa agtgctggcc cgcgctggcc
    16681 acggctcccg ccgtgaaatc gaatctatta ttgaagccgg tcgtgtgagt gttgatggca
    16741 aaattgctaa actcggcgat cgcgttgaag ttacccctgg gctgaaaatt cgtatcgatg
    16801 gtcatctgat ttcggtacgt gagtccgctg aacaaatttg tcgcgttctg gcctattaca
    16861 aaccggaagg tgagttgtgt acccgtaacg atccggaagg gcgtccgacg gtgtttgacc
    16921 gtctgccaaa gctgcgtggc gcacgctgga ttgccgtagg tcgtctggac gttaacacct
    16981 gtggtctgct gctgttcacc accgatggtg aactggcaaa ccgtttaatg catccaagcc
    17041 gtgaagttga acgtgaatac gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc
    17101 gtgatttgag tcgtggcgtg caactggaag atggtccggc agcttttaaa accatcaagt
    17161 tcagcggcgg cgaagggatc aaccagtggt acaacgtgac tctgaccgaa ggccgtaacc
    17221 gtgaagttcg tcgtctgtgg gaagcggttg gtgtgcaggt gagccgcctg atccgtgttc
    17281 gttacggtga tatcccactg ccaaaaggtc tgccacgcgg tggctggacg gagctggacc
    17341 tcgcccagac taactatctg cgcgaactgg tggagcttcc gccagaaacc agctctaaag
    17401 tcgctgtaga aaaagaccgt cgtcgcatga aggctaatca gattcgtcgt gcggtgaaac
    17461 gtcacagtca ggtgagcggc ggtcgtcgtt ctggcgggcg taataacggt taatcaaagt
    17521 atgccggacg tcatatccgg catttttaca gattaataat caatccctat ttgcgcctta
    17581 acaccggcat caaacgcatg tctgatgggg cgcagctcac ttaccgtatc cgccagttca
    17641 agaatatccc gatgacagcc tcgacccgtg ataatcaccg tctgttgatg tggacgttca
    17701 tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat atacgtcagt
    17761 tcatcaagca aaaccatatc cagtgaggaa tcagcaagca tccgctttgc atgttgccag
    17821 acttcgcggc aggcggcggt atcagactcg cggttttgtg tatcccaggt aaagcccgtt
    17881 gccatcacct gaaactcaac gccatgtggc tccagcagat tgcgttcgcc attaggccag
    17941 gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc gcgtgttgcc
    18001 gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat gataataccg
    18061 cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg ctgctgacgc
    18121 tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt tgggcgtcaa
    18181 aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag agcggcatga
    18241 tatcagcggg tgttttaagt ttctgtggat cttcggtcgg gaaggcgctt gcgcgcattg
    18301 cggtgcgcgt accgcctggg ttaatgcagt tgacgcgcag gcgctgctga tattcatcgg
    18361 ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgca ccccagttgg
    18421 ctcgtccctg acgtccaacg cttgatgaag taaagaccag tgaaccggcg tccgatttga
    18481 gtaataaagg aagcagtgcc tgggtgagca taaaggtggc attaacgttg acctgcatga
    18541 cgtcctgcca gacctgcgga ttttgttcgc tcattgggca aacatcgccg agcaatccgg
    18601 cattatgcaa aacaccatcc agacgcggat aattaacgac aatgcgctgt gccagttgtt
    18661 ggcaattttc ggacgtgcag gtcagcaaat cgagaataaa ccactgtggc tgacgcccag
    18721 tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg cccaacagaa
    18781 tcactgtcgc accatagcgt gcatacgtca tcgcggcttc acgaccaatc ccatcgctgg
    18841 ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg taatgcatgg
    18901 ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt tggggcttta
    18961 tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa aatggatact
    19021 ttgtcatact ttcgctgcaa taacatctct gcgagacggc ttaacatgcc tgttgtaaac
    19081 tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg
    19141 tttgtttttg gctaaaatcg ttaccgttgt gctagcgatt gcggcgattg ccgccattat
    19201 tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgggtca acaatctcag
    19261 cgaacagtat aaggagacga aagaagaact ggccgcggcg ctgatggact cacatcagca
    19321 aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag cagcaaaagc
    19381 gaaagccaaa ctgggcgagg tggcaactga cagtaaacct cgcgtctggg tgctggattt
    19441 taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa cggcggtact
    19501 cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagccctg gtggcatggt
    19561 gcatggttac gggttggcgg cttcgcagct gcagcgtctg cgtgataaaa acattccttt
    19621 aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct gtgtggcgga
    19681 caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc
    19741 caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca ccgccgggca
    19801 gtataagcgc acgctgacct tgctgggtga aaataccgaa gaagggcggg agaaattccg
    19861 cgaagagctg aacgaaacgc atcagttatt taaagatttt gtgaagcgta tgcgtccgtc
    19921 tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg cggtagagaa
    19981 aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga tggaaggccg
    20041 tgaagtggtt aatgtacgct atatgcagcg taaacgactc attgaccgat tcaccggcag
    20101 cgcggcagag agcgccgatc gattgttgct acgctggtgg cagcggggtc aaaagccatt
    20161 gatgtaaaag acaaacgcga ggctaagacc tcgcgttttg ctttaatcaa ccagatgata
    20221 tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg tgcttttggc
    20281 tgttggcaat ccttgttcat ctaaaaagta gtcgccggta aataccagca cgccattacg
    20341 ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac gaatgatttt
    20401 gttagcctct ttcaacagcg tttcgcggtc gatgggttgt gtctctttgt tcattattta
    20461 ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat ataccacgct
    20521 ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag ttgcgtatcg
    20581 gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg cttgacattc
    20641 gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga cctgttaact cagtcacctg
    20701 aattttcgtg aacagagtca cgacaagggg gtgatatccg cagagagcga gtccatatcg
    20761 gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt
    20821 aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa aagccaaaac
    20881 gatcaacaag tatctgggta gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga
    20941 tttgccgacc agtggctcag ctgccaaaaa gagtgccgac tctacctcca ccaagacggc
    21001 taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg
    21061 gcacaattgg gaggcgcact atgaagtgtt gcctagtaaa gagaaggtcg tctctgaact
    21121 gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg accgcgaagg
    21181 ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc gctatagccg
    21241 agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca aaccgggtga
    21301 gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg
    21361 gtatatggtt tcgccgctgc tgtggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt
    21421 gcagtcggtg gcggtccgcc tggtggtaga gcgtgagcgt gaaattaaag cgttcgtacc
    21481 ggaagagttc tgggaagtcg atgccagcac gactacgcca tctggtgaag cgttggcgtt
    21541 gcaggtgact catcagaatg acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc
    21601 tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac
    21661 aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg ccagcacccg
    21721 tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg aagcaggcta
    21781 tatcacttac atgcgtaccg actccactaa cctgagtcag gacgcggtaa atatggttcg
    21841 cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga atcagtacgc
    21901 cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg tcaatgtgat
    21961 ggcggaatcg ctgaaggata tggaagcaga tgcgcagaaa ctgtaccagc taatctggcg
    22021 tcagttcgtt gcctgccaga tgaccccagc gaaatatgac tccacgacgc tgaccgttgg
    22081 tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg gctggacaaa
    22141 agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag tcgataaagg
    22201 cgatgctctg acgctcgttg aactaacacc agcccagcac tttaccaagc cgccagcccg
    22261 tttcagtgaa gcatcgctgg ttaaagaact ggaaaaacgt ggtatcggtc gtccgtctac
    22321 ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag aaaatcgtcg
    22381 tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc cttgaagaaa atttccgcga
    22441 gttaatgaac tacgacttta ccgcgcagat ggaaaacagc ctcgaccagg tggcaaatca
    22501 cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc agcagttaga
    22561 taaagctgaa aaagatccgg aagagggtgg tatgcgcccg aaccagatgg ttctgaccag
    22621 cattgactgc ccgacttgtg gtcgcaaaat ggggattcgc acagcgagca ccggggtatt
    22681 ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca ccattaacct
    22741 ggtgccggaa aacgaagtgc tgaacgtgct ggaaggcgaa gatgctgaaa ccaacgcgct
    22801 gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc tcattgatcc
    22861 gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg agatcgaaga
    22921 gggcgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc
    22981 tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca acgaagagtg
    23041 taaaaacaca cgtaagattt tacgtaacgg cgaagtggca ccaccgaaag aagatccggt
    23101 gccattacct gagctgccgt gcgaaaaatc agatgcttat ttcgtgctgc gtgacggtgc
    23161 tgccggtgtg ttcctggctg ccaacacttt cccgaaatcg cgtgaaacgc gtgcgccact
    23221 ggtggaagag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt atctggccga
    23281 tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa
    23341 acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag cattttatgt
    23401 tgatggcaaa tgggttgaag gaaaaaaata acctttaatt ctatcaggtt tttataaaca
    23461 aagggtcgcg aaagcggccc ttttttattg catattattt tttcttcaca cctatacact
    23521 aaggctataa atgatatagt ggttatagtt agcacctttt ttattattaa atcgtattag
    23581 tcacccgcca ggtgtgacga aaaaacgatg ttctgatggc gtctaagtgg atggtttaac
    23641 atgaaattac aacaacttcg ctatattgtt gaggtggtca atcataacct gaatgtctca
    23701 tcaacagcgg aaggacttta cacatcacaa cccgggatca gtaaacaagt cagaatgctg
    23761 gaagacgagc taggcattca aattttttcc cgaagcggca agcacctgac gcaggtaacg
    23821 ccagcaggac aagaaataat tcgtatcgct cgcgaagtcc tgtcgaaagt cgatgccata
    23881 aaatcggttg ccggtgagca cacctggccg gataaaggct cgctgtatat cgccaccacg
    23941 catacccagg cacgctacgc gttaccaaac gttatcaaag gctttattga gcgttatccc
    24001 cgcgtttctt tgcatatgca ccagggcccg ccgacacaaa ttgctgatgc cgtctctaaa
    24061 ggcaatgccg atttcgctat cgccacagaa gcgctgcatc tgtatgaaga tttagtgatg
    24121 ttaccgtgct accactggaa tcgggctatt gtagtcactc cggatcaccc gctggcaggc
    24181 aaaaaagcca ttaccattga agaactggcg caatatccgt tggtgacata taccttcggc
    24241 tttaccggac gttcagaact ggatactgcc tttaaccgcg cagggttaac gccgcgtatc
    24301 gttttcacgg caacggatgc tgacgtcatt aaaacttacg tccggttagg gctgggggta
    24361 ggggtcattg ccagcatggc ggtggatccg gtcgccgatc ccgaccttgt gcgcgttgat
    24421 gctcacgata tcttcagcca cagtacaacc aaaattggtt ttcgccgtag tacttttttg
    24481 cgcagttata tgtatgattt cattcagcgt tttgcaccgc atttaacgcg tgatgtcgtt
    24541 gatgcggctg tcgcattgcg ctccaatgaa gaaattgagg tcatgtttaa agatataaaa
    24601 ctgccggaaa aataatttct tgcgttattt tcggcacctt ttatgtagcg aaggtgccgg
    24661 aatatattct cttttgttac ttatttctat accgtgcaca atatttttat ccctcatatt
    24721 tatagggtaa attacctgta aagtagtgtc taattacccg cctcgcaaat tccagttttc
    24781 aaatatctat ttcaggtcaa aagattgaat tattcactgg agacgattcg taaattcgct
    24841 ggatttttga ctaaagtttc tttaggattt gtctcatcgt atgattaatt acaccaatca
    24901 ttcaacgtct tgctgataag tgatggcgat tatatgaggt tagcaatgcc ttctggaaat
    24961 caggaacccc gccgtgatcc tgaattaaaa cgtaaagcct ggctgacggt ttttcttggt
    25021 tctgcacttt tctgggtggt tgtcgcactg ctgatttgga aagtgtgggg ataactatgg
    25081 tcggtcagga gcaactggag tcgtcaccat tatgccagca tagtgacaat gaaccagaaa
    25141 cgaaacggga atgttccgtc gttattccag acgactggca actaacatcg cagcagcaag
    25201 cctttataga actgtttgct gaagatgatc agccgaaaca ataattatca tcattcttat
    25261 tacccatttt taatgaatta aagggctttt aatacaccgc agcaataaca gcttgagtta
    25321 tctcaacaca aaataataac cgttaagggt gtagcctatg atcaacacaa atatgaaata
    25381 ttggtcctgg atgggcgcgt tttctctgtc gatgctcttc tgggccgaac tcctctggat
    25441 cattactcac tgatccttga ccccgctgcg gcggggttgt catttgcttt gccacaaggt
    25501 ttctcctctt ttatcaattt gggttgttat caaatcgtta cgcgatgttt gtgttatctt
    25561 taatatttac cctgaagaga atcagggctt cgcaaccctg tcattaagga ggagctatgt
    25621 cgtcaaccct acgagaagcc agtaaggaca cgttgcaggc caaagataaa acttaccact
    25681 actacagcct gccgcttgct gctaaatcac tgggcgatat cacccgtcta cccaagtcac
    25741 tcaaagtttt gctcgaaaac ctgctgcgct ggcaggatgg taactcagtt accgaagagg
    25801 atatccacgc actggcggga tggctgaaaa atgcccatgc tgaccgtgaa attgcctacc
    25861 gcccggcaag ggtgctgatg caggacttta ccggcgtacc tgccgttgtt gatctggcgg
    25921 caatgcgcga agcggttaaa cgcctcggtg gcgatactgc aaaggttaac ccgctctcac
    25981 cggtcgacct ggtcattgac cactcggtga ccgtcgatcg ttttggtgat gatgaggcat
    26041 ttgaagaaaa cgtacgcctg gaaatggagc gcaaccacga acgttatgtg ttcctgaaat
    26101 ggggaaagca agcgttcagt cggtttagcg tcgtgccgcc aggcacaggc atttgccatc
    26161 aggttaacct cgaatatctc ggcaaagcag tgtggagcga attgcaggac ggtgaatgga
    26221 ttgcttatcc ggatacactc gttggtactg actcgcacac cactatgatc aacggccttg
    26281 gcgtgctggg gtggggcgtg ggtgggatcg aagcagaagc cgccatgtta ggccagccgg
    26341 tttccatgct tatcccggac gtagtgggct ttaaacttac cggaaaatta cgtgaaggta
    26401 ttaccgccac agacctggtt ctcactgtta cccaaatgct gcgcaaacat ggcgtggtgg
    26461 ggaaattcgt cgaattttat ggtgatggtc tggattcact accgttggcg gatcgcgcca
    26521 ccattgccaa tatgtcgcca gaatatggtg ccacctgtgg cttcttccca atcgatgcag
    26581 taaccctcga ttacatgcgt ttaagcgggc gcagcgaaga tcaggtcgag ttggtcgaaa
    26641 aatatgccaa agcgcagggc atgtggcgta acccgggcga tgaaccgatt tttaccagta
    26701 tgttagaact ggatatgaat gacgttgaag cgagcctggc agggccaaaa cgcccacagg
    26761 atcgcgttgc actgcccgat gtaccaaaag catttgccgc cagtaacgaa ctggaagtga
    26821 atgccacgca taaagatcgc ctgccagtcg attatgttat gaacggacat cagtatcagt
    26881 tacctgatgg cgctgtggtc attgctgcga taacctcgtg caccaacacc tctaacccaa
    26941 gtgtgctgat ggccgcaggc ttgctggcga aaaaagccgt aactcttggc ctcaagcggc
    27001 aaccgtgggt caaagcgtcg ctggcaccgg gttcgaaagt cgtttctgat tatctggcaa
    27061 aagcgaaact gacaccgtat ctcgacgaac tggggtttaa ccttgtggga tacggttgta
    27121 ccacctgtat tggtaactct gggccgctgc ccgatcctat cgaaacggca atcaaaaaag
    27181 gcgatttaac cgtcggtgcg gtgctgtccg gcaaccgtaa ctttgaaggc cgtatccatc
    27241 cgctggttaa aactaactgg ctggcctcgc cgccgctggt ggttgcctat gcgctggcgg
    27301 gaaatatgaa tatcaacctg gcttctgagc ctatcggcca tgatcgcaaa ggcgatccgg
    27361 tttatctgaa agatatctgg ccatcggcac aagaaattgc ccgtgcggta gaacaagtct
    27421 ccacagaaat gttccgcaaa gagtacgcag aagtttttga aggcacagca gagtggaagg
    27481 aaattaacgt cacacgatcc gatacctacg gttggcagga ggactcaacc tatattcgct
    27541 tatcgccttt ctttgatgaa atgcaggcaa caccagcacc agtggaagat attcacggtg
    27601 cgcggatcct cgcaatgctg ggggattcag tcaccactga ccatatctct ccggcgggca
    27661 gtattaagcc cgacagccca gcgggtcgat atctacaagg tcggggtgtt gagcgaaaag
    27721 actttaactc ctacggttcg cggcgtggta accatgaagt gatgatgcgc ggcaccttcg
    27781 ccaatattcg catccgtaat gaaatggtgc ctggcgttga aggggggatg acgcggcatt
    27841 tacctgacag cgacgtagtc tctatttatg atgctgcgat gcgctataag caggagcaaa
    27901 cgccgctggc ggtgattgcc gggaaagagt atggatcagg ctcaagccgt gactgggcgg
    27961 caaaaggacc tcgtctgctt ggaattcgtg tggtgattgc cgaatcgttt gaacgaattc
    28021 accgttcgaa tttaattggc atgggcatcc tgccgctgga atttccgcaa ggcgtgacgc
    28081 gtaaaacgtt agggctaacc ggggaagaga agattgatat tggcgatctg caaaatctac
    28141 aacctggcgc gacggttccg gtgacgctta cgcgcgcgga tggtagccag gaagtcgtac
    28201 cctgccgttg tcgtatcgac accgcgacgg agttgaccta ctaccagaac gacggcattt
    28261 tgcattatgt catccgtaat atgttgaagt aacaagtatt tgcttgccgg ttattttgct
    28321 tccggcaagc aaatgaatta cacaatgcaa gagggttatt tgttcagcaa atggcccatt
    28381 ttctcggctt tggtatcgag ataatgttcg ttattggggt tacgacctac aatcaatggt
    28441 acgcgttcaa caatattaat ccctgcttcg gtcagaattt cgactttttt cgggttattg
    28501 gttaacaagc ggacttcatt gacgccaagg agtttgaaca tatcagcgca aagagtgaag
    28561 tcgcgctcat cagcggcgaa gcctaactgg tggttagcct ctacggtatc gtaaccttga
    28621 tcctgcagtg cgtaagcgcg gattttattc agcagaccaa tgttacgacc ttcctgacgg
    28681 tgatacagca aaataccacg gccttcctcg gcaatttgcg tcaatgccgc ttcgagctgg
    28741 aagccacaat cgcagcgcaa gctgaacagg gcgtcaccgg tcagacattc ggaatggacg
    28801 cgcgcaagta ccggggtatg cccggaaata tcggcataga ctagcgcgac atgatcgtgt
    28861 ccggttgcca gttcttcaaa tcccaccatc aggaaatcgc cccatggggt tggcagtttg
    28921 gcttctgcca cacgtttaag ctgcatgaaa ttctccagat aatgctggtt ctgtattggc
    28981 ttattttgcc ataacgagaa ggggttcacc taatcacgac gcgtcgatcg ttcacggaat
    29041 ggcacaaatc tgtcaatttt tcttggaact ggcattttca gttatgattg tgggacttat
    29101 caaaaaggag aggccatgcg ttcgattgcc cgacgtaccg cagtgggagc tgcactattg
    29161 tttgtcatgc cagtagccgt atggatttct ggctggcgtt ggcaacctgg agaacaaagt
    29221 tggcgactaa aagcggcttt ttgggttact gaaactgtca cccagccctg gggcgtcatt
    29281 acacatttga ttttgttcgg ctggtttctc tggtgtctgc gttttcgcat taaggctgcc
    29341 tttgtattat tttccattct ggcggcggca atccttgttg gacaaggcgt taaatcctgg
    29401 atcaaagaca aagtacagga accacgacct tttgttatct ggttggaaaa aacacatcat
    29461 attccggttg atgagttcta cactttaaag cgagcagaac gcggaaatct agtaaaagaa
    29521 cagttggctg aagagaaaaa tattccacaa tatttgcgtt cacactggca gaaagagacg
    29581 gggtttgcct ttccttccgg tcacacgatg tttgctgcca gttgggcact gctggccgtt
    29641 ggtttgctgt ggccgcgtcg gcgaacgtta accattgcta tcttgttggt ctgggcaacg
    29701 ggagtcatgg gaagccgcct gctactcggg atgcattggc cacgcgatct ggtggtagct
    29761 acgttgattt cgtggacgct ggtggcggtg gcaacgtggc ttgcgcaacg aatttgtggg
    29821 ccattaacac cacctgcgga agaaaatcgc gaaattgcac aacgagaaca agaaagttaa
    29881 cgctggttga ttttccgaat ttaggcctta aatcatcaac aatgcgcgtg gatgccattt
    29941 cgcagacggc gcgaaaatgg tactttaaag ggctattgcg gtaagttgac cataatttat
    30001 tcgctctaac cacataacgg gaagtaatgt gaaatattta ctcattttct tactggtgtt
    30061 agcgatcttc gtgatttcgg tcacgttggg tgcgcagaac gatcaacagg tgacgtttaa
    30121 ttatctgtta gcgcaagggg agtaccgtat ttccacattg ctggcggtat tgtttgctgc
    30181 ggggtttgct atcggttggt tgatttgtgg cctgttctgg ctgcgagttc gtgtttccct
    30241 ggcgcgcgct gaacgtaaaa taaagcgact ggaaaaccag ctttcacccg cgactgacgt
    30301 ggctgtagtg ccgcactcgt cagcggcgaa ggaataactt tctatgctgg agttgttgtt
    30361 tctgcttttg cctgtagccg ctgcctatgg ctggtatatg ggccgcagaa gtgcgcaaca
    30421 aaacaagcaa gatgaagcca accgcttgtc gcgtgattac gtagcggggg ttaacttcct
    30481 gcttagtaat caacaggata aagcggtaga cctgtttctc gatatgctta aagaggatac
    30541 aggcaccgtt gaagcccacc ttacgctcgg aaacctgttc cgttcgcgtg gcgaagttga
    30601 tcgcgctatt cgcatccatc agaccctaat ggaaagcgcc tcgctgacct atgaacagcg
    30661 tctgttggcg attcaacaac tggggcgtga ttacatggcc gccgggttat acgaccgcgc
    30721 ggaagacatg ttcaatcagc tgaccgatga aactgacttc cgcattggcg cgctgcaaca
    30781 gttgctacaa atctaccagg ctaccagtga gtggcagaaa gcaattgatg ttgccgaacg
    30841 cctggtgaag ctgggtaaag ataaacagcg cgtcgaaatt gcccatttct actgtgagtt
    30901 agccctgcag catatggcca gcgacgatct cgatcgtgcc atgaccttgc taaaaaaagg
    30961 ggcggcggca gataaaaaca gcgcccgcgt atccatcatg atgggacgcg tgtttatggc
    31021 gaaaggagaa tacgccaaag ccgtcgaaag tttgcaacgc gtcatatccc aggacagaga
    31081 actggtcagc gaaacgctgg aaatgttgca aacctgctat cagcagttgg gtaaaactgc
    31141 cgaatgggca gaattcctgc aacgcgcggt ggaagagaac accggtgccg atgctgaatt
    31201 gatgcttgct gatatcatcg aagcgcgcga cggtagtgag gctgcacagg tctatattac
    31261 acgccagctt cagcgtcatc cgaccatgcg tgtattccat aagctaatgg attaccactt
    31321 aaatgaagcg gaagaagggc gcgccaaaga gagtctgatg gtgctgcgtg acatggttgg
    31381 cgagaaggtg cgtagtaagc ctcgttatag ctgccagaaa tgtggtttta ccgcatacac
    31441 cctctactgg cattgtccgt cttgtcgtgc ctggtcaact attaaaccga ttcgcggtct
    31501 tgatggcctg taatttttaa aaaaatccga ctttagttac aacatactag ttattgatgt
    31561 cccattgtgc tccggcaacg acggagcaga aaaagcctgc taggggaaca atcgcaactg
    31621 ttaatttttt atttccacgg gtagaatgct cgccgtttac ctgtttcgcg ccacttccgg
    31681 tgcccatcat caagaaggtc tggtcatgac gttaactgct tcatcttctt cccgcgctgt
    31741 tacgaattct cctgtggttg ttgcccttga ttatcataat tgtgatgacg cgctgtcctt
    31801 tgtcgacaag atcgacccac gcgattgtcg tctgaaggtc ggtaaagaga tgtttacatt
    31861 gtttgggcca cagtttgtgc gcgaacttca acagcgtggt tttgatatct ttcttgacct
    31921 gaaattccac gatatcccca acactgcagc gcacgctgtc gctgctgcag ctgacttagg
    31981 cgtgtggatg gtgaatgctc atgcctccgg tggggcgcgt atgatgaccg cagcgcgtga
    32041 ggcactggtt ccgtttggca aagatgcacc gcttttgatt gctgtgacag tgttgaccag
    32101 catggaagcc agcgacctgg ccgatcttgg cgtgacactg tcacctgcag attatgcaga
    32161 acgtctggca gcactgacgc aaaaatgtgg ccttgatggt gtggtgtgtt ctgctcagga
    32221 agctgtgcgc tttaaacagg tattcggtca ggagttcaaa ctggttacac cgggcatccg
    32281 tccgcagggg agtgaagctg gtgaccagcg ccgcattatg acgccagaac aggcgttggc
    32341 ggctggtgtt gattatatgg tgattggtcg cccggtaacg caatcggtag atccagcgca
    32401 gacactgaaa gcgatcaacg cctctttaca gcggagtgca tgatgagtga ttccaacagc
    32461 cgtctggtct actcaacgga aaccgggcgt attgatgagc ccaaagcggc cccagtacgc
    32521 cctaaaggcg atggagcggt gcgtattcag cgtcagacca gtggacgtaa aggtaagggc
    32581 gtttgcctga ttaccggtat cgatctcgat gatgccgaac tgacaaaact tgcagcggaa
    32641 ctgaagaaaa aatgcggctg cggcggagca gttaaagatg gagttattga aatccagggc
    32701 gataagcgtg atttattaaa atcactactt gaagcgaaag ggatgaaggt aaaactcgca
    32761 ggcggttaac ataaaaagcc acggatatat ccgtggcttt cgaatatttt actgtgtgta
    32821 ttattcagtt atttcgtact gaacagaatc aagcgtatta taattgcgag tcgataatcg
    32881 acatttattt acgattattt accgacctgg tgaccaataa caccaccgac agctgcacca
    32941 cctaatgtac ccaacgtact gccatcggtc agtactgcac cgcctaatgc ccctgcaccc
    33001 gcgccgattg cggtgttgcg gtcccgttta gaccagttag aacaggcact cagagacatt
    33061 gccaaagtaa ttgccagaac agccgcggtc atttttttgc tcgttacaaa cataatactc
    33121 tctcctgaat ttatgattca cggaagtaag ctctctataa ctataatagc taagaataag
    33181 cctggtgaaa ttattccgtg aaatctgctc gcgcgcaggg ttatatcacg c
//
LOCUS       DAJTRQ010000029        32806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000029 DAJTRQ010000000
VERSION     DAJTRQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            16..4548
                     /locus_tag="OO332_001508"
     CDS             16..4548
                     /locus_tag="OO332_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR5942033.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4916..6571
                     /gene="lldP"
                     /locus_tag="OO332_001509"
     CDS             4916..6571
                     /gene="lldP"
                     /locus_tag="OO332_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5942034.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6571..7347
                     /gene="lldR"
                     /locus_tag="OO332_001510"
     CDS             6571..7347
                     /gene="lldR"
                     /locus_tag="OO332_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5942035.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7344..8534
                     /gene="lldD"
                     /locus_tag="OO332_001511"
     CDS             7344..8534
                     /gene="lldD"
                     /locus_tag="OO332_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5942036.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8730..9203
                     /gene="trmL"
                     /locus_tag="OO332_001512"
     CDS             8730..9203
                     /gene="trmL"
                     /locus_tag="OO332_001512"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5942037.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="OO332_001513"
     CDS             complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="OO332_001513"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5942038.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="OO332_001514"
     CDS             complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="OO332_001514"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5942039.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11176..11643)
                     /gene="secB"
                     /locus_tag="OO332_001515"
     CDS             complement(11176..11643)
                     /gene="secB"
                     /locus_tag="OO332_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5942040.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="OO332_001516"
     CDS             complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="OO332_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5942041.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12099..12530)
                     /locus_tag="OO332_001517"
     CDS             complement(12099..12530)
                     /locus_tag="OO332_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5942042.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12775..14319
                     /gene="gpmM"
                     /locus_tag="OO332_001518"
                     /gene_synonym="pgmI"
     CDS             12775..14319
                     /gene="gpmM"
                     /locus_tag="OO332_001518"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5942043.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14329..15612
                     /gene="envC"
                     /locus_tag="OO332_001519"
     CDS             14329..15612
                     /gene="envC"
                     /locus_tag="OO332_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5942044.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15616..16575
                     /locus_tag="OO332_001520"
     CDS             15616..16575
                     /locus_tag="OO332_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5942045.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="OO332_001521"
     CDS             complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="OO332_001521"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR5942046.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="OO332_001522"
     CDS             complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="OO332_001522"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5942047.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="OO332_001523"
     CDS             complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="OO332_001523"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5942048.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="OO332_001524"
     CDS             complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="OO332_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5942049.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21502..22434
                     /gene="rfaD"
                     /locus_tag="OO332_001525"
     CDS             21502..22434
                     /gene="rfaD"
                     /locus_tag="OO332_001525"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5942050.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22444..23490
                     /gene="rfaF"
                     /locus_tag="OO332_001526"
     CDS             22444..23490
                     /gene="rfaF"
                     /locus_tag="OO332_001526"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5942051.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23494..24486
                     /gene="rfaC"
                     /locus_tag="OO332_001527"
     CDS             23494..24486
                     /gene="rfaC"
                     /locus_tag="OO332_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5942052.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24483..25691
                     /gene="rfaL"
                     /locus_tag="OO332_001528"
     CDS             24483..25691
                     /gene="rfaL"
                     /locus_tag="OO332_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5942053.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25727..26869)
                     /locus_tag="OO332_001529"
     CDS             complement(25727..26869)
                     /locus_tag="OO332_001529"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5942054.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26878..27891)
                     /locus_tag="OO332_001530"
     CDS             complement(26878..27891)
                     /locus_tag="OO332_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5942055.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="OO332_001531"
     CDS             complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="OO332_001531"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5942056.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="OO332_001532"
                     /gene_synonym="rfaI"
     CDS             complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="OO332_001532"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5942057.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="OO332_001533"
     CDS             complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="OO332_001533"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5942058.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30489..31613)
                     /locus_tag="OO332_001534"
     CDS             complement(30489..31613)
                     /locus_tag="OO332_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5942059.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="OO332_001535"
     CDS             complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="OO332_001535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5942060.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8656 a         8034 c         7758 g         8358 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cgtctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgct ctgctttggg atgccaccgc aggtaaattc
     2161 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
     2221 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
     2281 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
     2341 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
     2401 accactcctg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
     2461 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
     2521 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
     2581 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
     2641 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
     2701 aacgccctgc tgtgggataa gactgccaat ggcggtgctg gagcctacaa tgccagccat
     2761 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
     2821 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     2881 attatcaatc agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt
     2941 atcaactacg ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt
     3001 gtcggcgcta cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt
     3061 ggtcagaaca gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc
     3121 agccgtgtga ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cttggtgatt
     3181 ggttatgaca ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa
     3241 taccgccaaa tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc
     3301 cagttgcaga acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac
     3361 tcaacggcag aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact
     3421 gtcgttaatg gtaatgcagg tattggtatc ggtttgaaca ctctggttct ggctgatgcg
     3481 atcaacggca ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg
     3541 ggtaacggtt ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac
     3601 gcaccgcaga actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc
     3661 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     3721 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     3781 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     3841 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     3901 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     3961 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     4021 atcaccaacg tagctgccag tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     4081 tcttctgaag caggcggagt gcgctacgac accaaagctg atggttctat cgactatagc
     4141 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     4201 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgcgca agaaacgaag
     4261 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     4321 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     4381 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     4441 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     4501 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     4561 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     4621 aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     4681 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     4741 cacaattccc caattaaaac tcacatcaat attgataata cataacattt agttaaccat
     4801 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     4861 aggagatgag caacagactc attacacgat gtgcgtggac tctaggagac ctgcaatgaa
     4921 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     4981 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     5041 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     5101 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     5161 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     5221 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     5281 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     5341 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     5401 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     5461 agtaacaggc atcgacagct ttgagattgg ccagatggtg gggcgtcagc taccgtttat
     5521 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga
     5581 gacgtggcct gcggtcgtgg ttgcaggcgg ctcgtttgcc atcgctcagt accttagctc
     5641 taacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     5701 gacgctgttc ctcaaacgct ggcaaccagt tcgcgtattc cgcttcggtg acttgggggc
     5761 gtcacaggtt gatataacgc tggcccacac cggttacacc gcgggtcagg tgttacgtgc
     5821 ctggacaccg ttcctgttcc tgaccgctac cgtaacactg tggagcatcc cgccgtttaa
     5881 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     5941 cctcaataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     6001 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     6061 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     6121 gaaagaacta gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     6181 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     6241 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     6301 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     6361 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     6421 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     6481 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     6541 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     6601 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     6661 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag
     6721 gcgctggcaa aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     6781 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     6841 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     6901 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     6961 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     7021 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     7081 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     7141 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     7201 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     7261 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     7321 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     7381 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     7441 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     7501 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     7561 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     7621 ccaaagcggc ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     7681 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     7741 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     7801 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     7861 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     7921 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     7981 aaccgaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     8041 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     8101 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     8161 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     8221 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     8281 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     8341 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     8401 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     8461 attcgctggt gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag
     8521 ggaatgcggc atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga
     8581 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     8641 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     8701 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     8761 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctacata
     8821 tcatcgaacc gatgggattt gcctgggacg acaagcgcct gcgccgcgcg gggctggact
     8881 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
     8941 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
     9001 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
     9061 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
     9121 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
     9181 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
     9241 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
     9301 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
     9361 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
     9421 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
     9481 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
     9541 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
     9601 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
     9661 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
     9721 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
     9781 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
     9841 ggcgatcatt tccgggtcag cggcgtaggc ttcttcaacc acttcacgga tagcaatagc
     9901 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt
     9961 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
    10021 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
    10081 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
    10141 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataatg
    10201 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
    10261 ttggcatttc aacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
    10321 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
    10381 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
    10441 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcacca agacgcgaca
    10501 tttcagccag cccacgggtg atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt
    10561 cagacatccc cgcgccaatg gcaatgacgt ttttcaccgc gccgccaagc tgcacgccaa
    10621 tgaaatccgg gttgctgtaa acgcggaaac ttttgccgca gtgcagcagc tgctggagat
    10681 catcggcaaa ggtctgatcg gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca
    10741 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
    10801 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
    10861 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
    10921 ggctgggtac gacgacgaga atattacgac tggctgccag cgcagtggcg agatcgcttt
    10981 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
    11041 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
    11101 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
    11161 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
    11221 caaatagttc atgaacagcg catcgaagtt aaccggcgca aggttcagtt gcgggaatgt
    11281 accgcgggat accatgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
    11341 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
    11401 gccctgctga acttcacaca ggaacgcggt ttcttcgccc aaggaggccg ttacggtaac
    11461 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
    11521 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
    11581 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
    11641 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
    11701 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
    11761 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
    11821 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg gaaactcacg
    11881 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    11941 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    12001 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    12061 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    12121 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    12181 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    12241 accgatttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    12301 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    12361 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    12421 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    12481 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    12541 aactcccaac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    12601 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    12661 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    12721 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    12781 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    12841 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    12901 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    12961 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    13021 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct ggctggcgcg
    13081 gtacataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    13141 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    13201 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    13261 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    13321 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    13381 gcctatgacc tgctgactct ggcgcagggt gagttccagg ccgatactgc cgttgctggt
    13441 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    13501 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    13561 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    13621 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    13681 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    13741 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    13801 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    13861 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    13921 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    13981 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    14041 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    14101 gctgaccacg gtaacgctga acagatgcgc gatccagcaa cgggtcaggc acacacggca
    14161 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    14221 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    14281 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    14341 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    14401 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    14461 cgtgaccaac tcaaatctat tcaggccgat atcgccgcaa aagagcacgc ggtacgccaa
    14521 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    14581 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    14641 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    14701 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    14761 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    14821 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt caccatgcag
    14881 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    14941 caacaggcga aactgactca ggttctgaac gagcgtaaaa agacgctggc ggggctggaa
    15001 tcttccatcc agcaaggtca gcagcagcta agtgaactac gtgccaacga atcccgtctg
    15061 cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    15121 gaggcccagg cggttcgcga gcgccagaaa gaagcgacgc gcaaaggcac cacctacaaa
    15181 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    15241 caggcattct ggccggttcg cgggccgacg ctgcatcgct atggcgagca gctacagggt
    15301 gaattacgct ggaaagggat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    15361 gccgacggtc gagtgattct ggctgactgg ctgcaaggtt acggtctggt ggtggtggtt
    15421 gagcacggta aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt
    15481 ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt
    15541 cggccttcac tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg
    15601 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    15661 gcgctctcct cccccgtact tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    15721 cgcccgcaca acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta
    15781 cccgattcac cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    15841 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctacgc
    15901 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    15961 tatgccgtgg ggatcaacaa ccacatgggc agcaagatga cctctaacct gtttggtatg
    16021 cagaaagtga tgcaggcgct ggagcgttac aatctttact tcctcgacag cgtaaccatc
    16081 ggtaataccc aggcgatgcg cgccgcgcaa ggtactggcg tgaaggtgat caaacggaag
    16141 gtgttcctcg acgattcgca gaacgaagcg gacatccgtg tgcaatttaa tcgcgcaatt
    16201 gatctggcgc gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta
    16261 cgcgtgctac aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    16321 agcttgctga atgaaccgca ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat
    16381 gcaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    16441 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    16501 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc acgaagcggc gaagtttaac
    16561 gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    16621 attgccataa gcggaaagac cacagtaacg cctggtaacc gaccttcacg ctgcgcacat
    16681 tggtaatcag gcgcttatac ataccggaag taaatatctc ggcaatcatc cgttggcggg
    16741 taagaatatc cggctctttg cgcaccgcat ggcaaacacg caatgcttcg taagttattt
    16801 gctgatgaaa ttccggataa atcgtaattt tgtcggcata atttcgattt aatttctcca
    16861 gcaggcgggt aatcttaata tagtgacgtt gataattaag gtttttattc ccttgtctat
    16921 gcaaccgact cactgacgta ttatgcagat aatatttata taatgattgc tcggtatatc
    16981 gcgcacgcag cgcgttaaac atgaattctg ttgtccagac aatatcctga tgatgtaatc
    17041 cggcaataaa tttaatgtta tttttaacaa taacatcacg gcgataaacc cccatccaga
    17101 caacgtgagt ccaacggcgc gaagaaagcc ccatccgcag ccagtccggg ccggttaata
    17161 caccggttga gcgaaggcga tcgctgggga tggattgcca ggtttctccc gtttcacgaa
    17221 aagaccagtc agcgttgcac tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca
    17281 gcgtttcgta catggtggga tagacctcat cgtcagcatc gacaaaagcg acatatttgc
    17341 ccgtcgccac ctcgatccca cgattacgcg ctaccgatgc gccagcattc gcctgatgca
    17401 acaaacgaac gtgcggatag ttttctgcgt aatgctttgc tatttcaaca gaattatccg
    17461 ttgaaccatc gttaataata atgatttcca gagcagtcca ggtttgcgta attaaagatt
    17521 ccatacaagt gcggaaatca tcgcccgcat tatataacgg aataataaca ctaagtttat
    17581 tggtgctgtt catcataaat taccgacgac ctctgaagtt atcctgacgt tttacaggct
    17641 ttgtattaag aaactattaa gcctgtgaga tattattttt acaggtgaga gagtcacttc
    17701 agggtagaga gataatgaga acagctatat aaaagatcag cgcaattacg aactctttat
    17761 tacactgaca ggattttaca taattaacag attatcctca gataaaaggg ctggaatacc
    17821 agcccttgtt cgtgttaatc ccagctcaga ataactttcc cggactggcc cgaacgcata
    17881 gcgtcaaagc ccttctggaa atcatcgata gagaaacgat gggtaatgat cggcgagaga
    17941 tcgaggccag actgaatcag cgccgccatc ttgtaccaag tttcaaacat ctcacgaccg
    18001 taaatgcctt taatgaacaa gcctttaaag atcactttgg tccagtcgat agacatatca
    18061 gacggtggaa tacccagcat cgcaatacga ccgccgtggt tcatggtatc gagcatggta
    18121 cgaaacgctg gcggcgcgcc ggacatttcc agaccgacat caaagccttc ggtcatgccc
    18181 agttcagcca tcacatcatt aaggttttct ttcgcgacgt taaccgcacg ggtgataccc
    18241 attttgcgcg ccagctcaag gcggtattcg ttaacatcgg tgatcaccac attgcgtgca
    18301 ccaacgtgtt tcgccaccgc cgcagccata atacctatcg gacctgcacc agaaaccagc
    18361 acatcttcgc ccaccagatc gaacgacagc gcggtatgca cggcgttacc gaagggatca
    18421 aaaatggaag ccaggtcgtc ggagatattg tcggggattt tgaaagcgtt gaacgccgga
    18481 atcaccagat attcggcgaa gcagcccgga cggttaacac cgacaccaat ggtgttgcgg
    18541 cacagatggg tacgaccgcc acgacagttg cgacagtgac cgcaggtgat atggccttcg
    18601 ccagaaacgc gatcgccgat tttgaagcct ttcacttcct gaccaatacc taccacttca
    18661 ccgacatatt catggcccac gaccatcggc accggaatgg ttttttgcga ccactcatcc
    18721 cagttataga tgtgaacgtc agtcccgcag atggctgttt tacggatttt aatcagcaga
    18781 tcgttatgcc cgagttccgg tacagggacg tcggtcatcc agatgccctc ttccgctttc
    18841 agtttggata acgctttcat ctcacatcct caggcgataa cgcccagttg tttaccaata
    18901 cgcgtgaatg cttctactgc acgcgtaatt tgctcagggg tatgcgccgc agacatctgg
    18961 gtacgaatac gcgcctgacc tttcggaacg accggataga agaaaccggt gacgtagatg
    19021 ccctcttttt gcagctctcg ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc
    19081 ggaataatgg cgtgatcggc tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg
    19141 aactgacgcg cgttcgccca cagacggtca cgcaactcgc tacccgcttc aaccatctcc
    19201 agcactttga tggaagcggc gacaatcgct ggtgccagcg agttggagaa caggtacgga
    19261 cgagaacgct ggcgcagcca ctcaaccact tctttgcgcg ccgcggtata accaccagaa
    19321 gccccgccca gcgctttacc gagcgtgccg gtgatgatgt cgacacgccc catcacatcg
    19381 cagtattcat gggaaccacg accattttca ccgacaaaac cgaccgcgtg ggagtcgtct
    19441 accatcacca gggcatcgta tttatctgcc agatcgcaaa cgcccttcag attggcaatc
    19501 acgccgtcca tcgagaacac accatcggtg gcaatcagca catgacgcgc accggcttca
    19561 cgcgcttctt tcagacgagc ttcaagctcc tgcatatcgt tgttggcata gcgatagcgt
    19621 ttagctttac acagacgcac accatcaata atagacgcgt ggttcagtgc gtcggagata
    19681 atggcgtctt ccgcgcccag cagcgtttca aacaggccac cgttagcatc aaagcaggaa
    19741 gagtagagaa tcgcatcttc catccccagg aatgccgcca gtttttgttc aagctcttta
    19801 tggctgtcct gagtgccgca aataaaacgc accgaagcca tgccgaaacc gtgagaatcc
    19861 attcccgcct ttgctgccgc aatcagatcc ggatgattcg ccagcccgag atagttgttg
    19921 gcacaaaagt taatgacgtg gcttccatca gccacggtga tatctgcttg ctgcgcagac
    19981 gtaataatgc gctcttcttt aaacaaccct tccgcccgtg cggtttccag atcgttggtt
    20041 aactgctgat aaaattctcc acgcattgcg attctccaga cagggcaaat tccagcacat
    20101 attacccaaa cttataggtc gcgacgagat gatgcgttaa cacttctgca aaattcagga
    20161 taaataacgt gtatcttgat gtctctcaac acctacagat agtagaaaaa ctgtacagtt
    20221 ttggctgagc ataatgatga gtgattttac tgcattgata taaaaagaaa aaatgatgcc
    20281 aaagcatcat ttttcctatc tcagttggca tcaaaacgct acccactaat ggagggaaac
    20341 ctattttctt gaaaggaaaa ttctcagcgc ctgcattttt gcccctaaag tgttactcct
    20401 gaagcagcga aacaaatcga accattttcc tctccccaga tagtttaggt tgaagagatc
    20461 ggaacgtttg taatagcaca tgacaaaaat gccctggtca tcgtccacaa tgttattgtt
    20521 tagtgttatt ttttgacttt ccagaactaa tttatagaat tctttccatt tatgctgtga
    20581 accaacaatt gcgcccccaa taatatatac atgattgcca atcatgaaat caaaagcctg
    20641 ttgttgagat gtaacagtaa gccctttttt tatagtgaaa agatgcatct tattttcatc
    20701 aaatgggaaa tcccaaattt tcaatcctcg agttacattt ggcttacggc aatagccaaa
    20761 atctatccac gcaacaagag gcgttttcac taaacccatg ttgatagctt tatttacaaa
    20821 ataggactta aggttacata ccaaaacata ctctggtgac cagtactctg ggttttttaa
    20881 ttgtcgaggc tcaagtctat ttgtaaacga ctcatctttt tgaatttttt cgatacgact
    20941 tctgatatat ctaaatttct tttttatatc gataacgata accgttgttg gttttccgtt
    21001 gcgaatcgct tcaatccttg gtttcaaatc gggagaagtg aaaataatca tctcattttc
    21061 aagtgcggcc agacgctcaa agtaactaaa ataaacatca actgaacgag ctagtttttc
    21121 acggaaccct ttattagtag tccaatcccc tctaccaatg tcaaaatatg cagtgataat
    21181 tgtggtggat gatttcatta ctaataagac cattaatata aaaaaagaat tatatcataa
    21241 atattaacag aatgtatact gtatattatc cattcaaatg attccgttat ttatcataca
    21301 tcggaatatt gatactaaag cactattcac atgcaaaacc aacatccgcc atgaaggact
    21361 agctaaaacc caaactagtt tgttgcaatt agcatccttg cacctctatg taaagggctg
    21421 aagggattcg gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc
    21481 cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc
    21541 aacatcgtta aggccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg
    21601 aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta tatggataag
    21661 gaagacttcc tgatccagat tatggctggc gaagagttcg gtgatgtcga agcgattttc
    21721 cacgaaggcg catgctcttc caccaccgag tgggatggca agtatatgat ggataacaac
    21781 tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc gttcctgtat
    21841 gcctcttccg cagccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac
    21901 gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata tgttcgtcaa
    21961 atcctgccag aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg
    22021 cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaat
    22081 aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga cttcgtctat
    22141 gtgggcgacg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggtatcttc
    22201 aacctcggca ccggtcgtgc agaatccttc caggcagtag cagatgctac gctggcttat
    22261 cacaagaaag gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag
    22321 gcgttcactc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa
    22381 accgttgctg aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc
    22441 tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt
    22501 ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca
    22561 tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc
    22621 ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag
    22681 cgctacgacc gcgcctacgt cttaccaaac tccttcaaat ctgcattagt gcctttcttc
    22741 gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc gctacggttt actcaacgat
    22801 gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc
    22861 tatgacaaag gcattatgcg taccgcacaa gatctgccgc agccattgtt atggccgcag
    22921 ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa
    22981 cgtccgatga ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac
    23041 taccactatg cggaactggc aaagcagctg attgatgaag gttatcaggt ggttctgttt
    23101 ggctctgcga aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag
    23161 caggcatggt gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt
    23221 gcagcctgta aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc
    23281 aatcgtccgc tggttgccct gtatggtcca agtagcccgg acttcacacc gccgctatcc
    23341 cataaagcgc gcgtgatccg tctgattacc ggctatcaca aagtgcgtaa aggtgacgct
    23401 gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact ggaagaactc
    23461 aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg
    23521 tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca
    23581 gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct
    23641 gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc
    23701 cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca
    23761 gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg tgacacgtct ggcgcatggc
    23821 gtaaagcatg gattggactg gcaaaccgct cgcgaacctt tagccagcct gttttacaat
    23881 tgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc
    23941 aaaagtttgg gctatagcaa accgcaaacc cagggcgatt atgctatcgc acagcatttt
    24001 ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt
    24061 gatgataaac actggccgga agaacactgg cgagaattga ttggtttact ggctgattca
    24121 ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg
    24181 gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc
    24241 gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg
    24301 gcactggata gacccaatat cacggtttat ggaccaaccg atccgggatt aattggtggg
    24361 tatgggaaga atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct
    24421 caaaccgttt ttcaaaattt aaatcttgaa ataataacca ataagttgac atcggagata
    24481 agatgacctc aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca
    24541 gagctctcgt attcttattc attaccacct attttttggg tgggattaca aggtataaac
    24601 atcttattgt tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac
    24661 actatctctc actatttaaa acatttcttt ttggtagtgt tgccatatta actattgctg
    24721 cattgctgtc acttcttcaa tcccctgatg caggtgctag catgaaggaa attttcaaag
    24781 ctattattga gaatacttta ctatgcacaa tagcaatacc ggtcatattg agagacgaga
    24841 aaagagaaga tgtcgaaaaa atcgttttct tctcatttat tagtgcgttg ggcttacgct
    24901 gtttttctga attgattacc tattataagg actatcaaca agggataatg ccattcgcag
    24961 attatagaca ccgtagcatt tctgactcga tggtcttttt attccctgca ttgttaaatc
    25021 tctggcttat caaatcagca aaataccgca tttcttttgt ggttctaagc gttattttta
    25081 tttttctgat attaggaact ttatccagag gggcctggct ttccgtgtta gtcattggat
    25141 taatatggat tctgatgttt aaacaatgga agttactatt agtaggagta atggtttcca
    25201 tcattgcatt gtcggttatt ttcacacata aggagatgac cgcaaagcta acgtataaac
    25261 ttcaacaaac taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc
    25321 taatattaga aaatcctgtt attggttatg gttacggtaa cgttgcatat aaagatgtct
    25381 ataataaacg tgtcattgat tatccagaat ggacctttag acaatcaata gggccacata
    25441 attttgcgct attcatctgg tttggcactg gtttattagg gctggtaagt cttatgatgc
    25501 tatactgtgc aatattgaaa gagtgtataa aaaatggcgt caagaataaa tatcgctcac
    25561 catataatgc atattatata atcttactat cttttatagg ttattttgtt atccgtggaa
    25621 acgtagaaca aattgaacca aatttattag gcgtttacgc cggcttatta ttagcgatga
    25681 aaaacaagta aaaaataaaa aaggctgcat aatgcggcct ttttatttat tcaaaccaat
    25741 tatgaataac ctcttcgaat ctttgagtta cgccatccca actgtagtga tcaaacacaa
    25801 agtcctgacc ttgctttgca actgctgtta attcaggatt cgctaacgtt tttaaaatat
    25861 cgctgcttat ggagtctgca gtcataggtt cttttaaatg gaatcccgta gtattttctt
    25921 tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac aggcttacca gcacccatag
    25981 cttcaattgc caccatgcaa aatggttcct ggaactggga aggaatgacg actaaatctg
    26041 ccagtgggta ataacaatgc atcttttcag gcggtatgct tcctaacata atgcaacgat
    26101 ccttcaaacg ttttgcaatt tccctgacat tgcgttgata tgcacctttg tcacttttac
    26161 tcatttctgt ataatcacca atcacaacta attttaaatt tttgtgagca gcggccaatt
    26221 tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc ccagcataaa
    26281 aaataatttt ctcttcaggg gagatattaa gttcgcttct agtgatcggc tgaaaattag
    26341 attgataggt ttctaaatca attccatttg gtacaatctc aatatctgca tttgctaaat
    26401 aagattggta atactttttc aagtataaac tagggactat cattttaaca ttttgctcaa
    26461 gcaattttgg ttcaaatgca ttatgcatat gtataaccac tcttgcctga ggagctcgct
    26521 tacgaatttg agtgtatagc ttcatgctat tatgaactac aataacactt tctttcgtta
    26581 taggaaaatc atgtgcaata ttcaatattc tctgtgaata tggaagagga tcaagtcttg
    26641 tccatttttg aaataaccgc ttatatatgc gactgaatcc aaccctatga atactacaat
    26701 tatcattaac aaaggtgtaa tttgagtatc ctggattttt gatacaaaca atacgatttg
    26761 gaaaattggt acgctgagca acctgataca tccacgtttc aacagcagcc gctccgcgag
    26821 gaggaattga aaaaatagga gtaaccgtaa atattatttt atcaaccatc tcgtatatta
    26881 acctttcata acattatatt taattgctgt aaaaacacca tcgagaaatt ttttctggta
    26941 aagcaagtgt ttatattttt ctttgtgctc atgtttttta acagcttttt tataaggtat
    27001 atttctccat ggtgatatat tataaatatt acgaaaataa tctgcagagg cgtagttcgc
    27061 ccaatcatgc catggcttag ttataccagt ataatgtatg aagacagtgt catcattaat
    27121 aaaccgggtg taatattcac tatttttttc ttcaaattct gactttatag tataaataca
    27181 attatatttt ctcggtaata ttttagcatg atgtagaaac attaagttta aaatatcttg
    27241 atccgggtac tttaattttt ttataattga ctcatctgat aatagatcaa agaatttttc
    27301 cgttagtctt tgttttaacc attcccgtaa attaatatac attacaccag agttaaaata
    27361 gctaccgtta aaatcctcat tacacaaacg ctcagcgctt ttactttgca tagcatctac
    27421 atcaattacc actgccccat attcatcttt aaactctaat gctattaact cgttcaatga
    27481 acccttacag acgatatcag catcaagata taataatgaa tccaatcgcg cagaaaaata
    27541 atcaaatgaa agcaaacgat aataaataga tactggccag atatttgatt gaggtagcgc
    27601 cttcaatggc tcagaatcaa tatgatataa tttaattact gtttgatact tcgttgctaa
    27661 ttgtgataat aattcaatat actctttgtc taagtaatca gcgattatgt gaaaggtaaa
    27721 tttgttttgt ttattctttt caagaattga agcaattgaa atcgcagccc caacctggta
    27781 gttctcatca ataccccatg agacattaaa cgtttcccgt tcatcaagtt taacggggcg
    27841 cttgtccagt tcaattatat ctttaaattg agtaagatgt ttaaaatcca atttaattac
    27901 ccttcatcat aatcattatt tgctcttcaa tcctagtttg acttttacat ccctgattac
    27961 ttttttaatt ttctttttga atataagata tgtatagcca aagtctttca attcattttt
    28021 tatattatag tgccgttcaa gatcaatgcg atcttttgct tttaaaacag cggttgtttt
    28081 tttacctgaa aggtcaatta gtcttaaacc tttttcggat actataaaat ttcctttatg
    28141 aggatcgcca gacaccattc catgctgatg caactctttt atcgattctg ataattgatc
    28201 cttcagatct tcagaaattt ccaaatactc atttaacccg acaccttcaa tatactcaat
    28261 gagcataata taataatgag cgaaatttaa tgttttacgt tcagctagca aaaaatagtc
    28321 attaattgat tggatgcctt caccacgtac ccgatcggtt tgataaatta aattttcata
    28381 ataatctttt tttatgcaag attttaagaa acgttcggtc attttatgct ttggcgcata
    28441 aactttaagt acaagaggtc cacgagcagt gtcaataaga atgactttag tatcatcaat
    28501 agaacgaaaa acttttaatg ttttaaccct gcaagttaaa aagtcattta gaacttgctc
    28561 ataaaaggga tcattatctt ttatgaaaac tttcaaccca tttatcgttt tattatatat
    28621 cataatgaat tattttaacc ttaaatcttt agaagcattt ttctttataa tactttaaat
    28681 aattaaaaat gcctgccata taatgctttt gtttaaattt atgttttgca caataccgat
    28741 actgattgct atttacaggt ttaagtaaat cttctttact ccacggagaa gctgctttgg
    28801 cgatcaaaaa actacgtgaa accggataat cagcccactc atgccagggt tttgtaggtc
    28861 ccacatagtg tataaacacg gtgtcatcat tgactggatt atcaactttg tcttttaatt
    28921 catagttaat actatatcgg gtattatatt tctccgaaat aaacttcact ttaccattca
    28981 ataatacatt cagtacatct tgatcaaggt gggtgatttt acttacccaa tctgggtcac
    29041 gcaacatttc aatagctttt gacgaaatgt tatttagatt ccactcatca atatttatca
    29101 gtaaaaaacc agcattgaaa tatccagaag ctaactgtgg tgtagttaat accgaggctc
    29161 gattttgcca ccattcaata tctctttcag ctacaactgc ggcaatttca ttagtagaaa
    29221 attgataatc taagagttct ttaatactac ccttgcaagc aatatctgca tcaagatata
    29281 gtattttttc atgtttatga tagaaataat ctgcaattat aaatcgaaaa tatgttgcgt
    29341 aagtccagtt tttcgtgctt ggtaatgact tcagcttatc acaattgata acataaatat
    29401 taattcttga attatattgt tttgctaaat cagaaaaata taatttgtct ttatcactga
    29461 tataatctgt gaaaacatga aattcacaag agatttctct attgtttaat agaatagatg
    29521 cgatggctac accacatcca aaaagaaaat ttctgtcaat accaaatgct atatcaagag
    29581 tatttttaga tcttatattt tgataatgaa aattatactc aaaaaggatg atgtcactat
    29641 cattgagttg agacatattt attctgtatc caaaattttg gcaaaaataa gttacgggtt
    29701 acaacgattt tcgaatcgtt ctttccctga tttttgtggc ttttgcttct gcttgcgaca
    29761 gcagtccttg ttcctgcttg agaatgtctt taagcggggc ggcaaaatac accttcataa
    29821 agcgccagat atcccgctga gtcaggccaa tattcattga agaaaaataa agccctataa
    29881 gatctttatc gcgccaacga cctggaacgc gcgtgcgaag ctgcgcccgg tgtaggtcaa
    29941 ttaccgaaat ttttaactcc tcttccttac cggaaaaagg caagtgcagc aggaaatgac
    30001 agatataaca gtcacgatgg ttaatgcccg cagcatgcat atcgcgcacc atcgtcgcga
    30061 cacgcttaat aagcatacgc tttacgcgaa catctggtgg gttggtcgcc cagtcagcac
    30121 agtaatcttc cagactgatg gttggtgtca gatcttcggt aataataaac gaggttctgg
    30181 tcagcggatt catgcctttt tcgccgaatg ccaccccata catagtatca acgccgacat
    30241 cccgcagtcg atgaatcgca ttccattcgc ggtctgcgcc taatactggc atccgcaatg
    30301 agagtaaatt tttgattatc tctttcaggg tcgtgccgcg atgccatttg agaaaatagc
    30361 tttttcccgc catttcaaag cgcagagtac ggcgagtttc cagttcacga aatacctcac
    30421 cctgcaaggt tttaacttcc tcaaaaggat ctttgccgcg ccataacgtg gcaaacggct
    30481 ctttaagttc aaccatccag accacccgtt atgatatccg ccgctttctc tggcagactg
    30541 tataaatctt gtgtatcagc ataatgtcgc gcattttccg cccaagcctg gcgcaatgaa
    30601 gattgcgtta acgctttgcg taaaatctca ttcaatgttt cctggcggaa tggctcagca
    30661 atagcctcgc cgcaattagc gtcgacaata taatgcgcat agccacaaac ggcagttgtt
    30721 agtaccggta atcctgcagt aatcgcttcc agcagcacaa ttcccgccgc ttcctggtag
    30781 gcaggatgca gtaataaatc cgccgccgcc attaattccg agacatcgtt gcgccccgag
    30841 aagaagtgaa cattactgcg cacgccgcgt ttttctgcca gtgcctcaaa ttttcgcggt
    30901 ttatcctgcc caacaacata tagcaatgtg ttgtggcgca gcgaatccgg taacgaagca
    30961 agtgcttcaa tggaacgatc gacaccttta cgcgtgaagt ctgaaccgac ctgcaacaat
    31021 aaatattgtt gttcggttat tccattcttc ttacggaaga tttcacggct attggctggc
    31081 tgctggctat atttacgatc aggataaatc cccggtggca gaatatgaaa acgctccgct
    31141 tcagtctgat aatgtttctg gaaatcggcg atttgcttat ctgtcagcat cagcagctgt
    31201 gtcggcttgc cctgttcgaa ggttgcccgc tcaaaggcgg cataatggcg ataacgtgac
    31261 gtcaggcgat agaaaaagcc tttttcctgc gctactttct cggcataaca aacatcagcg
    31321 gcataataaa cgtccagccc cggcattttg ttgaatccaa cgactttatc gacgggatgt
    31381 tcgcgtaaat gtttttgcac ccaggcaaaa tactccgcat tgcgcccgtg attggtatgc
    31441 gatttaaccg gcactttgat cagttcaaat acatcagggc attcgccttc ccacgactgg
    31501 gtataaaccc gaacatgatg acctcgggcg gcgactgtct gagcaatacg cataaaatcg
    31561 cgctgcaaac cgccaaaggg aaaatattta tataaacaaa aagcaacgat cataacgacg
    31621 tacctgtcgt ggaggagggc agtaatttat cgacagcagc aatgacatct gccgccggaa
    31681 taaccgaaag atacatctca tttcggtcac gctgatcgcg cgttggcatt tcccggtaat
    31741 ctcccgccca gaattgaatc atgttatttg accagggacg ccagaaaata tgatctgttg
    31801 cgccaaacag cgatatcagc ggcgtattaa ctgcagcggc aatatgcgcc ggtgcggaat
    31861 caacgccaat aaacagctgc gcatgatcga ttaacgcacc aagttccggg aaggtcacct
    31921 ttccagccag cgccgttact ggtggcgtct ggcatccctg cgcaatttca ttgacgcagg
    31981 ccagatcgtc tttatctggg ccggacgtca gaacgacttc ataaccacga gcatgtaagg
    32041 catcaatcac agcggaaaac ttggcgttgt cccagcattt gaagatttgc cgcgccgtag
    32101 gttggataac cacataattt tgtccaacac cagcgtgatc aagttcgcga cgcatacgtt
    32161 tccagcttgc aggcgggtaa ctcatggttg tctgcttcac caacgaatca agtcccaatg
    32221 gtgtcagcac ggataagtta ctttccacca catttccacc ctgcaacggc accaaatggg
    32281 tgaaactttt acgccaaaaa gcagactgcc gatgatgata atcctgggaa attttcacac
    32341 gagcatttaa taagcgaacc agtatagcaa ccatccattg atcggtaaga ttgacgataa
    32401 ggtcatactt attggcacgt aataccttga tgagatgaaa aaagttggca attttttctg
    32461 aggcttttgc ttttttattt tttatgccgt agagcgcgtt aatctctgga ttttcagaca
    32521 ggatcgggat ggtgtcctga taaagcagca catcgatttt tgcgtcaggg taattttttt
    32581 tcagagaact aatgacggga gtagttaata acatatcccc atgaaaacgc atcttaatga
    32641 gcaaaattct tcgaaatggc ttatccacaa gtaactcttt ttgttaagat tatccgatta
    32701 aggtgaagca gaaaaagcac gctaccgccc caggctcaac agctacctga atactgatac
    32761 cgcactgata ttgacgcgct agtgtatcac ttctttcgcg gcaatc
//
LOCUS       DAJTRQ010000030        32255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000030 DAJTRQ010000000
VERSION     DAJTRQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..725
                     /locus_tag="OO332_001536"
     CDS             <1..725
                     /locus_tag="OO332_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HCR5942061.1"
                     /translation="EDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWA
                     PHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLS
                     YITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIP
                     VLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRSFYGRHAVEVLY
                     QNQGALQRLLQLLEPYLPPKTH"
     gene            733..1212
                     /gene="coaD"
                     /locus_tag="OO332_001537"
     CDS             733..1212
                     /gene="coaD"
                     /locus_tag="OO332_001537"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5942062.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1251..2060)
                     /gene="mutM"
                     /locus_tag="OO332_001538"
     CDS             complement(1251..2060)
                     /gene="mutM"
                     /locus_tag="OO332_001538"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5942063.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2158..2325)
                     /gene="rpmG"
                     /locus_tag="OO332_001539"
     CDS             complement(2158..2325)
                     /gene="rpmG"
                     /locus_tag="OO332_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5942064.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2346..2582)
                     /gene="rpmB"
                     /locus_tag="OO332_001540"
     CDS             complement(2346..2582)
                     /gene="rpmB"
                     /locus_tag="OO332_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5942065.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(2799..3467)
                     /gene="radC"
                     /locus_tag="OO332_001541"
                     /pseudo
     CDS             complement(2799..3467)
                     /gene="radC"
                     /locus_tag="OO332_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            3639..4859
                     /gene="coaBC"
                     /locus_tag="OO332_001542"
     CDS             3639..4859
                     /gene="coaBC"
                     /locus_tag="OO332_001542"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5942066.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            4837..5295
                     /gene="dut"
                     /locus_tag="OO332_001543"
     CDS             4837..5295
                     /gene="dut"
                     /locus_tag="OO332_001543"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5942067.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            5402..5998
                     /gene="slmA"
                     /locus_tag="OO332_001544"
     CDS             5402..5998
                     /gene="slmA"
                     /locus_tag="OO332_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5942068.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6035..6676)
                     /gene="pyrE"
                     /locus_tag="OO332_001545"
     CDS             complement(6035..6676)
                     /gene="pyrE"
                     /locus_tag="OO332_001545"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5942069.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6742..7458)
                     /gene="rph"
                     /locus_tag="OO332_001546"
     CDS             complement(6742..7458)
                     /gene="rph"
                     /locus_tag="OO332_001546"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5942070.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            7585..8448
                     /locus_tag="OO332_001547"
     CDS             7585..8448
                     /locus_tag="OO332_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5942071.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            8669..9493
                     /gene="dinD"
                     /locus_tag="OO332_001548"
     CDS             8669..9493
                     /gene="dinD"
                     /locus_tag="OO332_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5942072.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            9784..10401
                     /locus_tag="OO332_001549"
     CDS             9784..10401
                     /locus_tag="OO332_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5942073.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10398..11915)
                     /gene="ligB"
                     /locus_tag="OO332_001550"
     CDS             complement(10398..11915)
                     /gene="ligB"
                     /locus_tag="OO332_001550"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5942074.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(11937..12194)
                     /locus_tag="OO332_001551"
     CDS             complement(11937..12194)
                     /locus_tag="OO332_001551"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942075.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12334..12957
                     /gene="gmk"
                     /locus_tag="OO332_001552"
     CDS             12334..12957
                     /gene="gmk"
                     /locus_tag="OO332_001552"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5942076.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13012..13287
                     /gene="rpoZ"
                     /locus_tag="OO332_001553"
     CDS             13012..13287
                     /gene="rpoZ"
                     /locus_tag="OO332_001553"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5942077.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13306..15414
                     /gene="spoT"
                     /locus_tag="OO332_001554"
     CDS             13306..15414
                     /gene="spoT"
                     /locus_tag="OO332_001554"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5942078.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            15421..16110
                     /gene="trmH"
                     /locus_tag="OO332_001555"
     CDS             15421..16110
                     /gene="trmH"
                     /locus_tag="OO332_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5942079.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16116..18197
                     /gene="recG"
                     /locus_tag="OO332_001556"
     CDS             16116..18197
                     /gene="recG"
                     /locus_tag="OO332_001556"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5942080.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18231..19436)
                     /gene="gltS"
                     /locus_tag="OO332_001557"
     CDS             complement(18231..19436)
                     /gene="gltS"
                     /locus_tag="OO332_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5942081.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            19716..21107
                     /gene="xanP"
                     /locus_tag="OO332_001558"
     CDS             19716..21107
                     /gene="xanP"
                     /locus_tag="OO332_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5942082.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21228..22937
                     /locus_tag="OO332_001559"
     CDS             21228..22937
                     /locus_tag="OO332_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5942083.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(22990..25308)
                     /gene="yicI"
                     /locus_tag="OO332_001560"
     CDS             complement(22990..25308)
                     /gene="yicI"
                     /locus_tag="OO332_001560"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5942084.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNDQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25318..26701)
                     /locus_tag="OO332_001561"
                     /pseudo
     CDS             complement(25318..26701)
                     /locus_tag="OO332_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            26994..27088
                     /locus_tag="OO332_001562"
     tRNA            26994..27088
                     /locus_tag="OO332_001562"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27028..27030,aa:Sec,seq:tca)
     gene            27388..28572
                     /locus_tag="OO332_001563"
     CDS             27388..28572
                     /locus_tag="OO332_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5942085.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSALIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            28984..30027
                     /locus_tag="OO332_001564"
     CDS             28984..30027
                     /locus_tag="OO332_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5942086.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            30542..30970
                     /locus_tag="OO332_001565"
                     /pseudo
     CDS             30542..30970
                     /locus_tag="OO332_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31000..32151)
                     /locus_tag="OO332_001566"
                     /pseudo
     CDS             complement(31000..32151)
                     /locus_tag="OO332_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8064 a         8502 c         8007 g         7682 t
ORIGIN      
        1 aagaagatgg tgcacgtttt gtggcgctgg gcgcaaaaaa taaccaggta accgttaccg
       61 gtagcctgaa attcgatatt tctgtaactc cgcagttggc tgctaaagcc gtgacgctac
      121 gccgccaatg ggcaccacac cgcccggtat ggattgccac cagcactcac gaaggtgaag
      181 agagcgtggt cattgccgca catcaggcat tgttacagca attcccgaat ttattgctca
      241 tcctggtacc ccgtcatcca gaacgcttcc cggatgcgat taaccttgtc cgccaggctg
      301 gactaagcta tatcacacgc tcttcagggg aagtcccctc caccagcacg caggttgtgg
      361 ttggcgatac gatgggcgag ttgatgttac tgtacggcat tgccgatctc gcctttgttg
      421 gcggttcact ggttgaacgt ggtgggcata atccgctgga agctgccgca cacgctattc
      481 cggtattgat ggggccgcat acttttaact ttaaagacat ttgcgcgcgg ctggagcagg
      541 caagcgggct tattaccgtt accgatgcca ctacgcttgc aaaagaggtt tcctctttac
      601 tcaccgacgc cgtttaccgt agtttctatg gccgtcatgc cgttgaagta ctgtatcaaa
      661 accagggcgc gctacagcgt ctgcttcaac tgctggaacc ttacctgcca ccgaaaacgc
      721 attgaggttg ttatgcaaaa acgggcgatt tatccgggta ctttcgatcc cattaccaat
      781 ggtcatatcg atatcgtgac gcgcgccacg cagatgttcg atcacgttat tctggcgatt
      841 gccgccagcc ccagtaaaaa accgatgttt accctggaag agcgtgtggc actggcacag
      901 caggcaaccg cgcatctggg gaacgtggaa gtggtcgggt ttagtgattt gatggcgaac
      961 ttcgcccgta atcaacacgc tacggtgctg attcgtggcc tgcgtgcggt ggcggatttt
     1021 gaatatgaaa tgcagctggc gcatatgaat cgccacttaa tgccagaact ggaaagtgtg
     1081 tttctgatgc cgtcgaaaga gtggtcgttt atctcttcat cgttggtgaa agaggtggcg
     1141 cgccatcagg gcgatgtcac ccatttcctg ccggagaatg tccatcaggc gctgatggca
     1201 aaattagcgt agcgtttatg ccggatggta tgccatccgg ctcgcatgaa ttacttctgg
     1261 cactgccgac aataaaacgt tgcccgctgc gcatgtttag tcgccacaat cggcgtaccg
     1321 cacacccgac acggctcacc ttttcgcccg taaacctgca attcctgcgc gaaatagccc
     1381 ggtttaccat cactttgcag aaaatctttc agcgttgtac caccctgctc aatcgaacgc
     1441 agcaacaccg ctttaatcac ccgagctaac aattcacact ctgccagcga cagtgatgac
     1501 gccagccgat ccggatggat ccccgccgca aacagtgatt cgctggcata gatattccct
     1561 acccctacca ccagcttgtt atccatcagc cacggtttaa tcgccgtttt tttcttcgcg
     1621 cacttctgat gcagatactc accattgaaa tcgtcgctaa gcggctccgg tccaagatgg
     1681 gccagcacat tatgcccttc cagctctttg gtccacagcc aggcaccaaa gcggcgcgga
     1741 tcggtgtagc gcagcacttt accgttgctc atcaccaaat ccacatggtc atgcttttca
     1801 gggggaagtt cttctggaag gatgcgcagg ctgccagaca tccccaaatg aatgataatc
     1861 cagccctcag gcagctccag cagcagatat ttagcccgcc gctgcacgct aagcactggt
     1921 tgatcgctta atcggtagat ctcttctgaa accggccagc gcaagcgtcc gttgcgcacc
     1981 accgcatgaa gaatggttgc accaacgaga ttcggttcta tgccgcgacg gctggtttca
     2041 acttcgggta attcaggcat agcatctcca ggaatgaaca gatgcaatca atatgggggc
     2101 aagcagataa caaaaaaccc cgccggagcg aggtttttcg ttacatcaaa gcgagaatta
     2161 tttgattttc gcttctttgt agatcacgtg ctgacgaaca actggatcga attttttcag
     2221 ttccagtttt tccggcttag tacgtttgtt cttcgtagtg gtatagaagt gaccagtacc
     2281 agcagaagaa accagcttga ttttctcacg aataccttta gccatgattt atttcctcta
     2341 agtacttagt acttttcgcc acgggcacgc agttcagcca gaactgtatc gatgcctttt
     2401 ttatcgatta cacgcatacc tttagcagat acacgcaggg tgacaaaacg cttctcgctc
     2461 tcaacccaga aacggtgaga gtgcaggttc ggcaggaaac ggcgtttagt cgcgttcagt
     2521 gcgtgggaac ggttgttacc ggtcaccgga cgcttgccag taacttggca gactcgggac
     2581 atgtctattc tccaaaaatc aaattagctc gagcttcgta tggggtattg gcgcctcgtc
     2641 aggctttaca gcccggtcat cgcagttcta tgtgaactct cgattgccag gcccaaatgc
     2701 caaacccgag attctcaaag gtggcgtagt atacgctgac tcagcgatgt gctcaagtcc
     2761 cgaacagaca aagatcccga aggatcgcgc atagcgggtt aaatccagcc gcgttcggca
     2821 aaagaaacat actctccacg cccaatcacg atatggtcga gcacgcgtaa atccatgaac
     2881 tgacaactct ttattatccg ttcagtaatg agtttatccg ctttactggg ttcagcacaa
     2941 cccgaagggt gattatgtgc aaggatcagc gccgaggcgt ttatttttat cgcttagcgg
     3001 ataatttccc gaggatggac ttcaacatgg tttagcgtgc cggaaaaaag acggctatgc
     3061 gttataaccc ggtgttgtga gtcgagaaag atcaccataa agatctcccg ctcctcaccc
     3121 gtgagctggc tttgtaaaaa ttcccgcgtc atctccgggc tgagtaaagg gctttcttca
     3181 cgcatccgca cgttgtagta acgtcgcgcc agttcagcaa tcccctttaa ctgggcaaat
     3241 ttcgctacgc caattccatg aacaccacta aattgttcat attcagaggt taacaagcca
     3301 taaagagagc cgaaattctc cagcatctct tttgccaggg ttaatacatc tttaccgcgc
     3361 gttccggtac gcagaaatag cgccagcagc tcgacatccg ttaaggcgct aataccaaac
     3421 ttcagcattt tttcgcgcgg catcaacagc tgtgcattgt ttttcacctt cacctccttt
     3481 gtggtgctcg catcctgtca tagccgttcc gttaaatcga cggccagttt tcaatcctgg
     3541 aaagagcctc gcaaagtgaa tctcaggtga aggcacgaca acaaaagtat tgtgataaaa
     3601 tcgccaactt ctggtgtcac ccaacaggaa aaatcatcat gagcctggcc ggtaaaaaaa
     3661 tcgttctcgg cgttagcggc ggtattgctg cctataaaac ccctgaactg gtgcgtcgtt
     3721 tgcgcgatcg cggggccgac gtccgcgtag ccatgaccga agcggcaaaa gcctttatca
     3781 ccccacttag cttgcaggcg gtttctggtt atcccgtttc cgacagtctg cttgacccgg
     3841 cagccgaagc agctatgggc catattgagc tgggtaaatg ggctgattta gtgattctcg
     3901 cccctgccac ggcagatttg attgcccgtg ttgctgccgg aatggcgaat gacctggtat
     3961 cgacgatttg tctggctaca cctgcgcctg tagccgtgct ccccgccatg aaccagcaga
     4021 tgtaccgtgc cgctgccacg cagcataatt tagaggtgct tgcttcccgt ggtttgctca
     4081 tctgggggcc agacagtggc agtcaggctt gtggtgatat cggtcctgga cgaatgctcg
     4141 atccgttaac cattgtggat atggcggtgg cgcatttttc gcccgtcaac gacctgaaac
     4201 atctgaacat tatgattacc gccggtccga cgcgtgaacc gctcgatccg gtgcgttata
     4261 tctctaatca cagctccggc aagatgggtt ttgctatcgc cgccgctgct gcccgtcgtg
     4321 gcgcgaacgt cacgctggta tcaggtccag tttcactacc gacgccaccg tttgttaaac
     4381 gtgttgatgt gatgaccgcg ctggaaatgg aagccgccgt gaatgcttct gtacagcagc
     4441 aaaatatttt tatcggttgc gccgccgtgg cggattatcg cgcagctacc gtggccccag
     4501 aaaaaatcaa aaagcaggcc acgcagggtg atgaattaac aataaaaatg gttaaaaacc
     4561 ccgatatcgt cgcaggcgtt gccgcactaa aagaccatcg accctacgtc gttggatttg
     4621 ccgccgaaac aaataatgtg gaagaatacg cccggcaaaa acgtatccgt aaaaaccttg
     4681 atctgatctg cgcgaacgat gtttcccagc caactcaagg atttaacagc gacaacaacg
     4741 cattacacct tttctggcag gacggagata aagtcttacc gcttgagcgc aaagagctcc
     4801 ttggccaatt attactcgac gagatcgtga cccgttatga tgaaaaaaat cgacgttaag
     4861 attctggacc cgcgcgttgg gaaggaattt ccgctcccga cttatgccac ctctggctct
     4921 gccggacttg acctgcgtgc ctgtctcgac gacgccgtag aactggctcc gggtgacact
     4981 acgctggttc cgaccgggct ggcgattcat attgccgatc cttcactggc ggcaatgatg
     5041 ctgccgcgct ccggattggg acataagcac ggtatcgtgc ttggtaacct ggtaggattg
     5101 atcgattccg actatcaggg ccagttgatg atttccgtgt ggaaccgtgg tcaggacagc
     5161 ttcaccattc aacctggcga acgcatcgcc cagatgattt ttgttccggt agtacaggct
     5221 gaatttaatc tggtggaaga tttcgacgcc accgaccgcg gtgaaggcgg ctttggtcac
     5281 tctggtcgtc agtaacacat acgcatccga ataacgtcat aacatagccg caaacatttc
     5341 gtttgcggtc atagcgtggg tgccgcctgg caagtgctta ttttcagggg tattttgtaa
     5401 catggcagaa aaacaaactg cgaaaaggaa ccgtcgcgag gaaatacttc agtctctggc
     5461 gctgatgctg gaatccagcg atggaagcca acgtatcacg acggcaaaac tggccgcctc
     5521 tgtcggcgtt tccgaagcgg cactgtatcg ccacttcccc agtaagaccc gcatgttcga
     5581 tagcctgatt gagtttatcg aagatagcct gattactcgc atcaacctga ttctgaaaga
     5641 tgagaaagac accacagcgc gcctgcgtct gattgtgttg ctgcttctcg gttttggtga
     5701 gcgtaatcct ggcctgaccc gcatcctcac tggtcatgcg ctaatgtttg aacaggatcg
     5761 cctgcaaggg cgcatcaacc agctgttcga gcgtattgaa gcgcagctgc gccaggtatt
     5821 gcgtgaaaag agaatgcgtg agggtgaagg ttacaccacc gatgaaaccc tgctggcaag
     5881 ccagatcctg gccttctgtg aaggtatgct gtcacgtttt gtccgcagcg aatttaaata
     5941 ccgcccgacg gatgattttg acgcccgctg gccgctaatt gccgcgcagt tgcagtaata
     6001 tgacgccgga tgacttttca tccggcgagt ttctttaaac gccaaactct tcgcgatagg
     6061 ccttaaccgc cgccagatgt tccgccattt ccggcttctc ttccagatag gcaatcagat
     6121 ctttcagggt gatgatagag atcactttgc agttgtaatc acgctcaact tcctgaatcg
     6181 ccgaaatctc gccgcgcccg cgttcctgac gatcgagcga aatcaacacg ccagcaagcg
     6241 tcgcgccatt ggcctgaata atctccatcg actcgcgaat cgccgttccg gcggtgatca
     6301 catcatctac cagcattacg cgtccttgta acgcgctacc aaccagattg ccgccttcac
     6361 cgtggtcttt tgcttctttg cggttaaagc agtacggcag gtccaggtcg tgatgctccg
     6421 ccagtgccac agcggttgtg gtggcaatcg ggatcccttt gtaagcaggg ccaaacagca
     6481 gatcgaactc aatgccggaa tccaccaacg cttcagcgta aaaacggcct aacagtgcca
     6541 gatcgcgccc ggtattaaac agcccggcgt tgaagaaata ggggcttttg cgcccggatt
     6601 tcagcgtaaa ctcgccaaac tttaacacct gcttgctaag cgcaaattca ataaactggc
     6661 gctgatatgg tttcatgcct tcgctcctca tcttactttt ctacagacaa aaaaaaggcg
     6721 actcatcagt cgccttaaaa atcagtttgc cagcgccgcc ttctgcgtcg ctacaatgga
     6781 ttcgattccc cctcgggcca gcgccaacaa ggtgagtagc tcttcatggg tgaacggctc
     6841 gccttctgcc gtcccctgca cttcaatgat gcgcccgtct tcggtcatca ctacgttcat
     6901 gtcggtctct gcggctgagt cttcaacgta ttccagatcg caaattgctt caccgtttac
     6961 aataccgaca gaaactgcgg ctaccatccc tttcatcggg ttggttttca gcttgccgtt
     7021 ttccaccagc ttctgtagcg catctgccag cgccacgcag gcacccgtaa tcgacgcggt
     7081 acgagtgcca ccatcagcct gaagcacgtc gcagtccagc gtaatggtga actcacccag
     7141 cgctttcaaa tctactgccg cgcgaagagc acgggcgatc agacgctgga tttccattgt
     7201 gcgtccaccc tgcttacctt tcgccgcttc acgagcgtta cgggtgtggg tagaacgtgg
     7261 cagcatgccg tactctgcgg tgatccagcc ctggccctga cctttcagga agcgcggcac
     7321 gccttcttca atagaggcgg tacacaatac tttggtatcg ccaaattcga ccagcaccga
     7381 gccttctgca tgttttgtat agttacgagt cagggtaacg ggacgcacct gattattgct
     7441 acggcctgct ggacgcatat tgaaatctcc ggcttgaaac aaatgtggct gcgcattata
     7501 cggacttccg gcggttattc ctatcctgac aaggcatcga tggctataat ccttccacct
     7561 ctccttttat aaacaggaac gtctatgatc cgcagtatga ccgcctacgc ccggcgtgaa
     7621 atcaagggtg aatgggggag cgcaacctgg gaaatgcgct cggtaaacca gcgttatctg
     7681 gaaacttact ttcgtctgcc ggagcagttc cgtagccttg aacctgtcgt tcgcgagcgt
     7741 attcgttctc gcctgacgcg cggtaaagtg gaatgtaccc tgcgctatga gccagatgtt
     7801 agcgcacaag gtgagctgat cctcaatgaa aaactggcta aacagctggt aactgccgcg
     7861 aactgggtaa aaatgcagag tgacgaaggg gaaatcaacc cggttgatat tctacgctgg
     7921 ccgggcgtga tggcagccca ggagcaggat ctcgacgcga ttgccgctga aattctcgcg
     7981 gcgttggatg gtacgctgga cgactttatc gtcgcgcgcg aaaccgaagg tcaggcactg
     8041 aaagcattga tcgagcagcg tctggaaggc gtcaccgccg aagtggtcaa agtccgcgcc
     8101 catatgccgg aaattctgca atggcagcgt gagcgtctgg tcgcgaagct ggaagatgct
     8161 caggttcagt tggaaaacaa ccgtctggag caggagttgg ttctgctggc acaacgaatt
     8221 gacgttgccg aagagctgga ccgcctcgaa gcgcatgtta aagagacgta caacattctg
     8281 aagaaaaaag aagcggttgg tcgtcgtctg gactttatga tgcaggagtt caaccgtgag
     8341 tcgaacactc ttgcgtcgaa atctatcaat gccgaagtga caaactccgc catcgagctg
     8401 aaagtgttga ttgagcagat gcgcgagcag attcagaata ttgagtaact cgtaacacca
     8461 attctgactt ttcagccttc acaaatgccg cccactcaaa cagagcggca tttttcttcc
     8521 ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt ttttgcccag
     8581 cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga tttactttct
     8641 ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt ttgaagagat
     8701 aaaactgatt aatgcaaacg gagcagaaca atggtcagca agacaacttg ggaaactact
     8761 gggttattca gagtatcgtc actttatacc tgtattaacg cgcgccaaag aggcctgtga
     8821 gaacagtggt cacacaattg atgaccattt cgaggagatc ctcgatatgg tcaaaattgg
     8881 ctcaaatgcc aaaagagcat taaaagacat cgtactctcc cgctatgcct gttacctggt
     8941 agtacaaaac ggcgaccccg cgaaaccggt cattgcggca gggcagactt attttactat
     9001 ccagacccga cggcaggagc ttgctgacga tgaagcattt aagcaacttc gtgaagacga
     9061 aaagcgtctg tttctgagaa atgagttgaa agaacataac aaacaattgg ttgaggcagc
     9121 acagcaagct ggggtagcga cagctactga tttcgccata tttcagaatc atggttacca
     9181 ggggctgtat ggtggattag atcagaaagc catccaccaa ctgaaggggc tgaaaaagag
     9241 tcagaagatc ctggatcata tgggttcaac agaactggcg gctaatctct ttcgagctac
     9301 ccaaacagaa gaaaaactca agcgggatag cgtgaattca aaacagcaag ctaataccac
     9361 acactttgac gtgggtagca aggtgaggca aaccattcag gaacttggcg gaaccatgcc
     9421 tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg taaaaattac
     9481 agagaagaaa tagcagaaac gaaaaactct ttttgttgat tgagacaccc gatgcgtaag
     9541 ccaaggtcca ggtgcagtga acacaatggc taaacattgc acctttcttt tcccccctca
     9601 gttttaacct attttttctt atgcattttc tcagacaaga agtcagaaga atgcatctac
     9661 gctacagaaa atagcgattt cacataacta aaagttatca atttcctctc accttaaaaa
     9721 atatcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt tagggagaga
     9781 accatgctgt tacacatttt gtatttggtt ggcattactg ccgaagccat gacgggggcg
     9841 ctggcggccg ggcgacggcg catggataca tttggcgtaa ttattattgc aaccgccacc
     9901 gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct cggctgggtc
     9961 aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac tatcgttgct
    10021 cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg actggtggtc
    10081 ttttctatca tcggcgcaca ggttgcactg gatatggggc acggcccaat tattgccgtt
    10141 gtcgcggcgg taaccaccgg tgtatttggc ggcgttttgc gcgatatgtt ctgtaaacgc
    10201 attccgctgg tctttcaaaa agagttgtat gccggtgtct catttgcctc cgccgtgctg
    10261 tacatcgcgc tgcaacacta tgtttctaac catgatgtgg tgattatctc caccctggta
    10321 ttcggctttt ttgctcgttt actggcgcta cgtcttaagc tgggattacc ggtcttttac
    10381 tacagccacg aaggtcacta aggttcaaaa cctgtgatct gctgggcagc cagccaactg
    10441 cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc gcgcccagat
    10501 ccagtccccg gcagctgctg ccagaactgc tccgtgctaa ataaaagttg cgaccaggat
    10561 cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc catcacccag
    10621 cgagtaaaag gctggttacg agccagatta aactgatgcc ataactgcgc acttttactt
    10681 ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca ggaaaagata
    10741 tgctcaaagc gatgagtctg atgcagcgcg cgccaaccgg cctcaccaat gccatccagc
    10801 cccagaacct gttttgaccc cagccagact aagcgtgaaa tgaactgttc ctgacaaaca
    10861 tcagaagcaa agtagcaggt caacgagtta aagcggtttt ctggtggtgt cggttttgta
    10921 cgttctgcac cgcgccagac cacatcatca atgcgaggaa taccctgacc gccaaggctg
    10981 acgaggatct gatcaccagg cgcaatatcc cactcctgcc agcgcctgac ggaaccaata
    11041 ttcacccgct ggactttttt atcatccagc atgacaggtg cgagtgacgc aaccaccgat
    11101 attttaccgc tcttacccac cgcaaactga atcgccttca cttcggcaac ctgagctaca
    11161 ggttgatatt tccaggccac cagccactcc gcctggcccg gtagccaatg acgggattcg
    11221 ggttccttag cccctcgcac aaccacgcca tcggtgacga agggtaattt cgctttccac
    11281 cactcattgc gtacgcgcgc aacctcatca gcatttttaa ccgcacgggt atacctctgc
    11341 gttagagtaa aacctgcggt agccagctcc tttaaacgat cggtcattaa ctgcggtcca
    11401 tccggccatg cccagacaaa aacagccagt gagtttagcg tgtcgctatt cccctggcgc
    11461 atcatcaggc cagcaacttt tgcgcgggca tttattcccc ccatttgttg ttggatatgc
    11521 ccctcgcgct ggagaaatat ttccccctga agcgtactat tggctaaagg cccgctaacg
    11581 gtttgcggca cagcggaaat taagctaact ttctgcgtcc agtcctcgcc tttcaggcca
    11641 ttaccgcgac tgattgcttt gttcagtttc ccgtcccgat aaaccagggt taccgcaacg
    11701 ccatcaactt ttggctgcac ccaaagatca ctacgttctc gcatccacag acttaatgcg
    11761 tttttatccg ccattttgcg tacacccgta tgggcaaccg gatgcataac cgcaccattt
    11821 aaaggcggca tcatcacgtc gcggggctcg ctcacaaagc agcgttgcca ctgcgtaaga
    11881 cgggcgctta actgatcgta aacaccgtct tccacctcac tttttccttc cagtagtcat
    11941 cgtcccactg ttttatttgc tgttgcaggc gggaaatttc ttcctgtgct ctggctggcg
    12001 accaggccgg acagaccgcc cacacagatg attgccagca caagatactt attaatatcg
    12061 ccatccatac tttcatcttc acctccgctg tagtcaggct ggcagatata caacgtggtg
    12121 aaggcaaagc cgagtggcaa aaacggagtc tgcgaggacg cttcctgaga atcgtcttta
    12181 ttgcagtaaa tcacaggcaa atgcggaaac agctacgcaa aacgcaacaa ctttgcgcaa
    12241 aaagtgtgag caagggctac gtcacatggc cgcgccgtgt ataataagct cgtatgtagg
    12301 ctttatttcg ctaatcacat acgaaagata ctcatggctc aaggcacgct ttatattgtt
    12361 tctgccccca gtggcgcggg taaatccagc ctgattcagg ctttattaaa aacccaaccg
    12421 ttgtatgaca cccaggtttc tgtttcacac accacacgcc aaccgcgtcc tggtgaagtc
    12481 cacggtgaac attatttctt tgttaatcat gatgaattta aagaaatgat tagcagagat
    12541 gcgttcctcg aacacgcaga agtttttggt aattactatg gcacttcgcg tgaggccatt
    12601 gagcaagtac tggcgaccgg tgtcgatgtt tttctcgata tcgactggca gggcgcgcag
    12661 caaattcgcc agaagatgcc gcacgcgcgg agtatcttta ttttaccgcc gtccaaaatt
    12721 gaactggacc gccgtctacg cggtcgcggt caggacagcg aagaggtcat tgcaaagcgt
    12781 atggcgcaag ctgttgcaga aatgagccat tacgccgaat atgattatct gattgtgaat
    12841 gatgacttcg ataccgcgtt gaccgatttg aagaccatta ttcgcgccga acgtctgcgc
    12901 atgagccgcc aaaagcagcg tcatgacgct ttaatcagca aattgttggc agactgaacc
    12961 tgatttcagt atcatgccca gtcatttctt cacctgtgga gctttttaag tatggcacgc
    13021 gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt actggtcgcc
    13081 gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc ggaagaaaac
    13141 gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa caaccagatc
    13201 ctcgacgttc gcgaacgcca ggaacagcaa gagcaggaag ccgctgaatt acaagccgtt
    13261 accgctattg ctgaaggtcg tcgttaatca caaagcgggt cgcccttgta tctgtttgaa
    13321 agcctgaatc aactgattca aacctacctg ccggaagacc aaatcaagcg tctgcggcag
    13381 gcgtatctcg ttgcacgtga tgctcacgag gggcaaacac gttcaagcgg tgaaccctat
    13441 atcacgcacc cggtagcggt tgcctgcatt ctggccgaga tgaaactcga ctatgaaacg
    13501 ctgatggcgg cgctgctgca tgacgtgatt gaagatactc ccgccaccta ccaggatatg
    13561 gaacagcttt ttggtaaaag cgtcgccgag ctggtagagg gggtgtcgaa acttgataaa
    13621 ctcaagttcc gcgataagaa agaggcgcag gccgaaaact ttcgcaagat gattatggcg
    13681 atggtgcagg atatccgcgt catcctcatc aaacttgccg accgtaccca caacatgcgc
    13741 acgctgggct cacttcgccc ggacaaacgt cgccgcatcg cccgtgaaac tctcgaaatt
    13801 tacagcccgc tggcgcaccg tttaggtatc caccacatta aaaccgaact cgaagagctg
    13861 ggttttgagg cgctgtatcc caaccgttat cgcgtaatca aagaagtggt gaaagccgcg
    13921 cgcggcaacc gtaaagagat gatccaaaaa atcctctcgg aaatcgaagg gcgtttgcag
    13981 gaagcgggaa ttccctgccg cgtcagtggt cgcgagaagc atctttattc gatttactgc
    14041 aaaatggtgc tcaaagagca gcgttttcac tcaatcatgg acatctacgc tttccgcgtg
    14101 atcgtcaatg attccgacac ctgttatcgc gtgctcgggc aaatgcatag cctgtacaag
    14161 ccgcgtccgg gtcgcgtgaa agactacatc gccattccaa aagcgaacgg ctatcagtct
    14221 ttgcacacct cgatgatcgg cccgcacggt gtgccggttg aggtccagat ccgtaccgaa
    14281 gatatggatc agatggcgga gatgggtgtt gccgcgcact gggcttataa agagcacggc
    14341 gaaaccagca ccaccgcgca aatccgcgcc cagcgctgga tgcaaagcct gctggagctg
    14401 caacagagcg ccggtagttc gtttgaattt atcgagagcg ttaaatccga tctcttcccg
    14461 gatgagattt acgttttcac accggaaggg cgaattgtcg agctgcctgc cggtgcaacg
    14521 cccgtcgact tcgcttatgc agtgcatacc gatatcggtc atgcctgcgt gggcgcacgc
    14581 gttgaccgcc agccttaccc gctgtcgcag ccgcttacca gcggtcaaac cgttgaaatc
    14641 attaccgcac cgggcgctcg cccgaatgcc gcgtggctga actttgtcgt tagctcaaaa
    14701 gcgcgcgcca aaattcgtca gttgctgaaa aacctcaagc gtgatgattc tgtaagcctg
    14761 ggccgtcgtc tgctcaacca tgctttgggt ggtagccgta agctgaatga aatcccgcag
    14821 gaaaatattc agcgcgagct ggatcgcatg aagctggcaa cgcttgacga tctgctggca
    14881 gaaatcggac ttggtaacgc aatgagcgtg gtggtcgcga aaaatctgca acatggggac
    14941 gcctccattc caccggcaac ccaaagccac ggacatctgc ccattaaagg tgccgatggc
    15001 gtgctgatca cctttgcgaa atgctgccgc cctattcctg gcgacccgat tatcgcccac
    15061 gtcagccccg gtaaaggtct ggtgatccac catgaatcct gccgtaatat ccgtggctac
    15121 cagaaagagc cagagaagtt tatggctgtg gaatgggata aagagacggc gcaggagttc
    15181 atcaccgaaa tcaaggtgga gatgttcaat catcagggcg cgctggcaaa cctgacggcg
    15241 gcaattaaca ccacgacttc gaatattcaa agtttgaata cggaagagaa agatggtcgc
    15301 gtctacagcg cctttattcg tctgaccgct cgtgaccgtg tgcatctggc gaatatcatg
    15361 cgcaaaatcc gcgtgatgcc agacgtgatt aaagtcaccc gaaaccgaaa ttaatgtttt
    15421 atgaacccaa cacgttatgc acgcatctgc gaaatgctcg ccaggcggca gcctgatctg
    15481 accgtctgca tggagcaggt ccacaaacct cataacgttt ctgcgattat tcgtaccgca
    15541 gatgccgttg gcgtacatga agttcacgcc gtctggcctg gtagccgcat gcgcaccatg
    15601 gcttcggcag cggcgggtag taacagctgg gtacaggtga aaacacaccg caccattggc
    15661 gatgccgtcg ctcatctcaa aggccagggc atgcagattc tggcaaccca tctttctgat
    15721 aatgctgtcg atttccgcga aattgattac actcgcccga cctgcatttt gatgggacag
    15781 gagaaaacgg gcatcacgca ggaagcattg gccctggcgg atcaggacat catcattccg
    15841 atgatcggca tggtgcagtc gctgaatgtt tccgttgcct cagccctcat tctttacgaa
    15901 gcccagcgtc agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat gttgccggaa
    15961 gcagagcaac aacgcctgtt gtttgaaggc ggctatccgg tgctggcgaa agtcgcaaaa
    16021 cgcaaaggcc tgccttatcc ccacgtcaat cagcaaggcg agatcgaagc tgatgccgac
    16081 tggtgggcta ctatgcaggc tgcagggtaa gtgccatgaa aggtcgcctg ttagatgctg
    16141 tcccactcag ttccctaacg ggcgttggcg cagcacttag taacaaactg gcgaaaatca
    16201 acctgcatac cgtgcaggat ctactcttac accttcccct gcgctacgaa gatcgcaccc
    16261 atctctaccc catcggagaa ctactgccgg gcgtttatgc cacggtggaa ggcgaagtgc
    16321 tgaactgcaa tatctccttc ggcggtcggc ggatgatgac ctgccagatc agcgacggtt
    16381 ccggcatcct caccatgcgc tttttcaatt tcagcgcggc aatgaaaaat agcctggcga
    16441 cgggccgccg tgtactggct tatggcgaag caaagcgcgg taaatatggt gcggagatga
    16501 tccacccgga ataccgcgtg cagggcgatc tcagcacgcc agaattacag gaaacgctca
    16561 cgccggttta tccaacaacg gaaggcgtaa agcaggccac gctgcgtaaa ttaaccgacc
    16621 aggcgctgga tctgctcgac acctgcgcca ttgaagaact cctgccgccg gaactgtcac
    16681 aaggaatgat gacgctaccg gaagcgttgc gcactttgca ccgcccgcca ccgacgctac
    16741 agcttagcga tctggaaacc gggcagcatc cggcgcaacg tcgtctgatt ctggaagaac
    16801 tgctggcgca caacctcagc atgttagcct tacgtgccgg agcacagcgt tttcatgccc
    16861 agccgctgag cgccaatgac acgctgaaaa ataaactcct cgccgcctta ccgttcaagc
    16921 caacgggcgc acaggcacgc gtagtggcgg agatcgagca tgatatggcg ctggatgtgc
    16981 cgatgatgcg cctggtacag ggcgatgtag gttccggtaa aacgctggtc gccgccctcg
    17041 ccgcgctgcg tgcgattgcc cacggcaaac aggtggcact gatggcacca accgaattac
    17101 tcgccgagca gcacgccaat aacttccgca actggtttgc accgctcggt atcgaagtgg
    17161 gctggctcgc cggtaagcag aaaggcaaag cgcgactgtc tcagcaggaa gccatcgcca
    17221 gcggtcaggt gcagatgatt gtcggtacac acgccatctt ccaggaacag gtgcagttta
    17281 acggcctggc gctggtgatt atcgacgaac agcatcgttt tggcgtgcat cagcgtctgg
    17341 cattgtggga gaaaggccag cagcagggct tccatccgca tcagttgatc atgaccgcca
    17401 cgccgatccc ccgcacgctg gcaatgacgg cgtatgccga tctcgatacc tcggtgatag
    17461 atgagctgcc gccaggccgc acgccagtga ctacggtcgc tattcctgat acccgccgta
    17521 ccgacatcat tgaccgcgtg cgccacgcct gcataactga aggtcgtcag gcgtactggg
    17581 tttgtacgtt gattgaagag tcagagttac tggaagcgca ggcggcggaa gctacctggg
    17641 aagagttgaa actggcgcta ccagaattga atgttggcct ggtacacggg cggatgaaac
    17701 cagccgagaa acaggcggtg atggcgtcgt ttaaacaagg tgagctacat ctgctggttg
    17761 ccactaccgt tattgaagtc ggtgttgatg tgcctaacgc cagcctgatg attatcgaaa
    17821 accctgagcg tctgggtctg gcgcagttac accagctgcg tgggcgcgta ggtcgtggcg
    17881 cggtggcttc tcactgcgtg ctgctctaca aaacgccgct ttctaaaacg gcgcaaattc
    17941 gcctgcaagt gctgcgcgac agtaacgacg gttttgtgat tgcgcaaaaa gatctggaga
    18001 ttcgcggccc tggcgaattg ttaggcacgc gtcagacggg taatgctgaa tttaaagtgg
    18061 cggatttact gcgcgatcag gcgatgatcc cggaagttca gcgcctggcg cgacatattc
    18121 acgaacgtta cccacaacag gcaaaagcct tgatagaacg ctggatgccg gagacagaac
    18181 gttactcgaa tgcgtaaaag gcggcggtga gaagaccgcc gcttcatcgg ttaaccggca
    18241 aaaatcggca acattaaata cagcttaatc accagcgcat tgacgatatc gataaagaac
    18301 gcaccgacca tcggcaccac caaaaacgcc atgtgcgacg ggccaaagcg ttcagtgatc
    18361 gcctgcatgt tggcgattgc cgttggcgtt gcaccgaggc caaaaccaca gtgacccgca
    18421 gccagcactg ccgcatcgta gtttttgccc atcatgcgcc aggtaacgaa gatggcatac
    18481 aacgccatga agatggtctg taccaccaga atcgccagca tcggcagtgc cagcgaagcc
    18541 agctcccaca gtttcagccc catcaacgcc atcgccagga acaacgacaa gcttacgtta
    18601 cccagcacgg ataccgcacg ctcaaagacg cggtaaaagc ccattattga cagaccgttg
    18661 ctcagaatca cgccaacaaa cagtacacag acgaaggtcg gcagttcaaa agcagtgcca
    18721 gccaaaagtt gcgcaacaat tttccccacc gtcaggcaga tagcaatcag cgcgatagtt
    18781 tcaatcagca ccaacgaggt gatcatgcgt cccacatccg gcttttcaaa cgccgtcggg
    18841 acttcctggt catccggaat accgttcggc gtggtggagt gtttcaccag atagcgcgcc
    18901 accggaccgc caatcaagcc gcccagcacc agaccgaacg ttgcacaggc catagccact
    18961 tccgtcgcat tggtgaagcc ataacgttca atgaacaatt tactccacgc agcgcccgta
    19021 ccgtgaccgc cggaaagcgt aatagaaccg gccaacagcc ccatcagcgg atcaagccct
    19081 aacaggctag ccataccaat gccaatggca ttttgcatca ccaacagacc aacaaccaca
    19141 atcaagaaga tgccaaccac acgcccaccg gcacgcaaac tggcaatgtt ggcgttcagg
    19201 ccaatggtgg cgaagaaagc cagcattaac ggatcgcgca gggacatatc aaagttgact
    19261 tcccagccca tgcttttttt cagtactagt agcgccagcg ccaccaacaa accacccgca
    19321 acaggttccg gtatggtgta tttcttcaaa aaggagacgg aatggaccaa cttacgcccg
    19381 agcagcaacg tcagcgttgc ggcaacaagc gttgctaaag tatcgagatg aaacatagtt
    19441 actcctttgt atccgcatca ctctttcata ctcgtcatac ttcatcgcgc agtggcaact
    19501 tcatgaacta tgacgggtat cgagttatgc tttcctgggt caaattctca gctgttaatg
    19561 aacaaatggc atggatttta agcagaaaaa taccaaaagt tatataaaaa agtgcattta
    19621 tctcatttaa atatttagat gaggatgtgg caaacgtttg cttttctctt tcggtcagct
    19681 aaaatgcccg ctttgctacc acgggattgt tttcaatgtc tgtttccacc ctcgagtcag
    19741 aaaatgcgca accggttgcg cagactcaaa acagcgaact gatttaccgt cttgaagatc
    19801 gtccgccgct tcctcaaacc ctgtttgccg cctgtcagca tctgctggcg atgttcgttg
    19861 cggtgatcac gccagcgcta ttaatctgcc aggcgctggg tttaccggca caagacacgc
    19921 aacacattat tagtatgtcg ctgtttgcct ccggtgtggc atcgattatt caaattaagg
    19981 cctggggtcc ggttggctcc gggctgttgt ctattcaggg caccagcttc aactttgttg
    20041 ccccgctgat tatgggcggt accgcgctga aaaccggtgg tgctgatgtt cctaccatga
    20101 tggcggcttt gttcggcacg ttgatgctgg caagttgcac cgagatggtg atctcccgcg
    20161 ttctgcatct ggcgcgccgc attattacgc cgctggtttc tggcgttgtg gtgatgatta
    20221 tcggcctgtc gctaattcag gtggggctga cctccattgg cggcggttac gcagccatga
    20281 gcgataacac cttcggcgca ccgaaaaatc tgctgctggc aggcgtggtt ttagccttaa
    20341 ttatcctgct taaccgtcaa cgtaaccctt acttacgcgt ggcctcactg gtaattgcga
    20401 tggcggccgg atatgcgctg gcgtggttta tgggcatgtt gccagaaagc aacgaaccga
    20461 tgacgcaaga actgattatg gtgccaacgc cgctctatta cggtcttggc attgaatgga
    20521 gtctgctgct gccgctgatg ctggtcttta tgatcacttc gctggaaacc attggcgata
    20581 tcacggcgac ctctgacgtt tccgaacagc cagtgtccgg tccgctgtac atgaaacgcc
    20641 tgaaaggcgg cgtgctggca aacggcctga actcgtttgt ttcggcggtg tttaacacct
    20701 tcccgaactc ctgcttcggg caaaacaacg gagtgatcca gttgactggt gttgccagcc
    20761 gctatgtcgg ttttgtcgtc gcgctgatgt tgatcgtgct gggtctgttc ccggcagtga
    20821 gcgattttgt acaacacatt ccagaaccgg ttctgggcgg cgcaacgctt gtaatgtttg
    20881 gcaccatcgc cgcctccggt gtgcgtatcg tttctcgtga gccgctgaac cgtcgggcga
    20941 ttctgattat cgcgttgtcg ctggcggttg gtctgggcgt gtctcagcag ccgctgattt
    21001 tgcagtttgc ccctgaatgg ctgaaaaacc tgctctcctc cgggatcgcc gcgggcggta
    21061 ttactgccat cgtgctgaat ctgattttcc caccagaaaa acagtaattc actggcggcg
    21121 gtaagataat taccgccgca gtctcacctc tttcaccatt cccgccttga ggattgcgcg
    21181 taaatcgtgc ataactccct tatgtgcatt tcacgggatg gaagaccatg aaatttattg
    21241 ggaagctgct tctctacatt ctcatcgctc tgttagtggt gatcgctggc ctctattttc
    21301 ttctgcaaac ccgctgggga gcagaacata tcagcgcatg ggtttccgag aatagcgact
    21361 atcatctggc cttcggggcg atggatcacc gtttttccgc gccatctcat atcgtgctgg
    21421 agaatgtcac gtttggtcgt gatggtcagc ccgcgcccct ggtggcaaaa agtgtcgaca
    21481 ttgcgctaag cagtcgccaa ctgaccgaac cacgccatgt cgataccatc ctgctggaaa
    21541 acgggacgct gaatctcacc gaccagaccg cgccgctacc gttcaaagcc gatcgtctgc
    21601 aactgcgtga tatggcgttt aatagcccga atagcgaatg gaaactgagc gcgcagcggg
    21661 taaatggcgg cgtggttccg tggtcaccag aagccggtaa agtgctgggt acgaaggcgc
    21721 agattcagtt tagtgccgga tcgctttcgc tcaatgatgt tcctgccacc aatgtactga
    21781 ttgaaggcag tattgataac gatcgcgtta cgctgactaa cctgggtgcc gacatcgccc
    21841 gcgggacatt aaccggaaac gcgcagcgta acgccgacgg cagctggcaa gtggaaaatc
    21901 tgcgcatggc ggatatccgt ctacaaagcg aaaaatcgct aaccgacttc tttgcgccat
    21961 tacgctctgt cccgtcgttg cagattggtc gcttggaagt catcgatgct cgtctgcaag
    22021 gtccggactg ggcggtgacc gacctcgatc tcagcttgcg caacatgacc ttcagtaaag
    22081 atgactggca gacacaggaa ggcaaactgt cgatgaacgc cagcgagttc atttatggtt
    22141 cgctgcattt atttgacccg attataaacg cggaattttc cccgcagggc gtagcgctgc
    22201 gccagttcac cagccgctgg gaagggggta tggtcagaac gtcagggaac tggctgcgtg
    22261 acggtaaaac gttgatcctt gatgatgcgg caattgccgg gctggaatat accttgccga
    22321 aaaactggca acagttgtgg atggaaacga cacccggttg gttaaacagc ctgcaactga
    22381 agagatttag cgccagccgc aatctgatca ttgatatcga ccctgacttc ccgtggcagc
    22441 tcaccgcgct cgatggttac ggtgccaacc tgacgctggt taccgatcat aaatggggcg
    22501 tctggagtgg ctcggcgaat ctgaatgccg ccgccgcgac attcaatcgt gttgatgttc
    22561 gtcgcccgtc gctggcgctg accgccaaca gcagcacggt gaatatcagc gaactgagtg
    22621 catttactga aaaaggcatt ctggaagcca ccgcaagtgt ttcacaaacg ccacaacgtc
    22681 agacccatat cagcctgaat ggacgcggtg tgccggtgaa tattttgcaa caatggggat
    22741 ggcctgaatt acagttgact ggcgacggca atattcagct taccgccagt ggagatattc
    22801 aggccaatgc cccgctgaaa cctacggtta gcgggcaatt gcatgccgtg aacgccgcaa
    22861 agcagcaagt gactcaaacc atgaatgcgg gcgtcgtttc cagcggtgaa gttacatcga
    22921 cggagccggt gcggtaaaca tcaatctcgc cgggtacgac tcagacgtac ccggcattcc
    22981 gtcaatagat tacaacgtaa ttgtcagcgc attcccttga ggcgtcacca ccagcccctg
    23041 ctcactttca gcctgcgaac cgccttgcag accatttact ttcacaacat tgcgcaagca
    23101 cagcgtccag ttcttcgcct cgcccgcacc agtcacagta atcgtgttgc cagtacgtgc
    23161 tgcttttaaa gtaaagatca ccgatccgtc agcagcaggc acttcacaga cggcttcatg
    23221 cccgtcttgc agattaaaga ggtggaatgc cgtgccttcg tgccacacgt aatcgggacg
    23281 ttgatcgttg ttgcccagcg ccagtagagt gttatcacgc acataaacgg gcagactcag
    23341 gaagccgtgc tgctgtttat gccagcgact accgtcgagt tcatcgttgt gccacaggtg
    23401 tgtccagcga ccttccggca ggtagaactg cacatcgccc gcttcagtga acaccggcgc
    23461 aaccatcacg ttgtcgccta acatgtattg acggtcaagg taatcacaag ccggatcgtc
    23521 cgggaactcc atcatcatgg cccgcatcat cggcgtaccc cgcgcgttcg cacgcgcagc
    23581 ttcacgatac agatacggca tcatgcggca tttcagttgc gtgaagaagc gcaccacatc
    23641 acaggactca tcatcgtagg cccacggcac acgataagat ttgctaccgt gtaaacggct
    23701 atggctggag agcaaaccaa acgcgcacca gcgtttgtaa acgtgcgccg gagcggtatt
    23761 ttcaaagccg ccgatatcgt ggctccagaa gccaaaacct gaaaggccaa tagacaaacc
    23821 accgcgcagg ctttccgcca ttgattcgta gttagcgtaa caatcgccac cccagtgtac
    23881 cgggaatttc tgcgcaccga cggaggccga gcgggcaaac aagacagctt cttcctcacc
    23941 aacggtgtcc ttgagcacgt tccacaccag ttcgttgtag atgtacgcat aatggttatg
    24001 cattttctgc ggatcggaac cgtcaaacca ctgaacatca gttgggatac gttcgccaaa
    24061 gtcggtctta aagcaatcaa cgcccatcgc gaccagacct ttcagtttgt cggcgtacca
    24121 tttgcaggca tccggattgg taaagtcata aatcgccaga cctggctgcc atttatccca
    24181 ctgccatagc gaaccgtccg ggcgtttgag taaatagcct ttctcttgta actctttaaa
    24241 gacgggggat ttttgaccga tataggggtt aatccagacg cagattttca gccctttcgc
    24301 tttcaggcgg cggatcatcc cttccgggtc agggaaagtc agcgggtccc actcaaaatc
    24361 gcaccactgg aaggctttca tccagaaaca gtcaaagtgg aaaacatgca gcggcagatt
    24421 gcgttccgcc ataccatcga taaagctgtt taccgtcgct tcgtcgtagt tggtggtaaa
    24481 tgaagtggtt agccacaggc cgaaggacca cgggggcggc agcgccggac gaccagtaaa
    24541 gcgggtataa cgatcaagta ccgctttcgg cgtcgggccg tcgataacaa agtattcgag
    24601 atattcactc tcaacgctga actgcacttt ggagactttc tccgatccca cttcaaaaga
    24661 gacacactgg ggatgattga ccagtacccc ataaccacgg ttagtcatgt agaacgggat
    24721 atttttatac gcctgttcag tacttgtgcc gccgtcccgg ttccaggtct ctaccgtctg
    24781 gtcattgcgc accagggcag taaagcgctc tcccagaccg taaactgttt cgccaacgcc
    24841 aagatcaagc cgctcaaaca tataattgcg ttgattattc gtgtcctgca cgtagccatt
    24901 atttttcacc tgactaccgg taatacgttc gccgttgcgc agaaaatcca gtgaccagaa
    24961 ctcacctttg ctgacacgcg cgcttaagtt gccactttta aactcagcat aacgttctgt
    25021 gttttcgatt gtgaccttca cgtcctgcaa aatattgagc ggataatgag gaccgttatt
    25081 cagcgccccc tgaaaatact caatccgcac accgacaata ccttcctgtg gggagaaaaa
    25141 gcgcagcgta aataaaggcg tatcaagctg ccaggtacgt tcacgcacat cacggggggc
    25201 agcatagacc accatttcat tatcctgctg ttcaacctcg aatacctgaa gcgggtgaat
    25261 caaattgagc ccaggttgaa tcaaccagtt tccatcgcta attttcattg ccgttcctta
    25321 gttctgcact tcttgagagg tgaattgttg ctggcaacgg cgtttaccct gagccagctg
    25381 ttccataacg gttttcagat tgtgcgtcgt gagtgagtag tagcgtttag cgataatcgc
    25441 gctcagcaaa taacagatcg ccggaacaat cgtgaatagc gcaataatga tgctaatcgt
    25501 ggcgctgttc tgcgcttttt ctgccgcatc atatccgcca taagccagca tccagccgat
    25561 aagagcgccg ccgaaggcca accccagttt gagcacaaac agcgtgccag caaaactgat
    25621 cccggtcagg cgtttaccat tgcaccactc gccgtagtcg acggtatcga acatcattac
    25681 ccactggata ggtgtcacca gttgatgcaa cacaccaatc acgaagatga agacaaacat
    25741 agtgatgctg gcctgcatgg gaacaaagaa catcgcgagg ctaatcactg ccagcagggc
    25801 gttcgtccac cagaagatag tgactttaca tttccagtcg gtcagaggtt ttgccagtgc
    25861 ggaaccaatc aggttaccca cgcaataagt ggtgagaaaa gcgacaaaca cttccggcgt
    25921 gcccaaaatc catgtgacgt aatacatcat cgccccaccg cgtacgcaca ccgccaggat
    25981 attgaaaatg gttagtaaac cgacaatccg ccactggtcg ttttgccaga tatcacgtaa
    26041 atcttcccgc atagacgttg ttgtaggtgg tgcttcaacg cgttctttag tggtgaagaa
    26101 acaaaatgcc agcatcatta atgccaccac ggaaaggacc acgataccgc cctggaaacc
    26161 gagtggttta ttatcaccgc caattaaatt aaccagtggc atcatcagaa cagtagaaag
    26221 catgcctccc gcggtcgcca gcacaaaacg ccaggattgc agcgagatac gctgagtcgg
    26281 gtcattggtg attacaccac ccaatgcgca gtaagggata ttgacgacgg tataaagtaa
    26341 ggtaagttag ggtgtaagta attgctgcat agatcatttt gccgttcata ctgagatctg
    26401 gcgtgctata ggccagtaca cagacgatcc cgaatggcag tgcgccaaac agtacccacg
    26461 gacgaaattt accccagcga gagcgcgttc gatcggccaa caaccccatg caaggatcgg
    26521 aaatcgcatc cagtgcacga gcgaccaaaa acatggttcc gacaaatccg gcaggaatgc
    26581 caaaaatatc ggtataaaag aacatcatat ataacattac gttatcgaaa ataatgtggc
    26641 tggcggcgtc tcccatgcca taaccaattt tctctttaac ggacaacact tcactcttca
    26701 tctgcttttc cttgatgcta taggggtgct gagaccgata acgcattttg taaaccatcc
    26761 ctgtgagagc gaatattccg atatctggtt attaatttat gattcttgtt ttatgtgatc
    26821 gtggtagcgt taattccgct catatatcat tgtaaaatat gggttttata taaattataa
    26881 tgctttcgtg ataatacgct gcgtttatta ggcggaaaaa actgatctgg gggatgtaga
    26941 aactcaagga agtagctata atgcgccccg cctccatgta gcaatcgagg cgcggaagat
    27001 cgtcgtctcc ggtgaggcgg ctggacttca aatccagttg gggccgccag cggtcccggg
    27061 caggttcgac tcctgtgatc ttccgccagt ttacctcttc tgaactctcc caatgtcaat
    27121 aaatctcttc taatcccttg tatatcagta tttatttgta tcctgaggtc aacctacttc
    27181 accccgtatc aagtttcttt tgggggtact ttagggggtg ttatttccta ttgaaataca
    27241 agcaccccca taataggaaa aatgtgatac ataacaacgg cttttaacaa tatctttgtt
    27301 atacaagggg tgtattgggg gtatcaggtt agttactggc agaaatcccc ctcaggaaca
    27361 tgaattcatc aaaagccgga ggatgtcatg gcattaaccg atatcaaagt gaaaaccgca
    27421 aaacccaaag acaaacccta taagcttgct gatggcggcg gtatgtatct actgattaac
    27481 actaatggtt ctaaatactg gcgcatgaag taccgctttg ctggtaaaga aaagatgctt
    27541 tctattggcg tataccccga tgtaacgcta gccgatgctc gcgagaaacg cagtgaagcc
    27601 aggaaactcc tcgctgctgg aggcgatcca ggggaggcaa aaaaggaaga aaaaatcgcg
    27661 cagcaaatga gtctgaaaaa tacgttcgag gctattgctc gcgaatggca ccagttaaaa
    27721 gctgatcggt ggtcattacg ctatcgtgat gaaatcattg atacctttga aaaagacatt
    27781 tttccctaca ttggcaagcg ccctattgcc gaaatcaaac caatggagtt actggaagca
    27841 ctgcgtaaaa tggaaaaacg tggagctctg gagaaaatgc gcaaggtccg ccagcgctgt
    27901 ggtgaggtgt tccgctatgc aattgttact ggccgggcag actataaccc agcccccgat
    27961 cttgccagtg ctttagctac accgaaaaaa gtacattttc cctttcttac tgctaatgaa
    28021 cttccccact tcctcaacga tctggcgggt tataccggaa gtataatcac taagacagct
    28081 actcagatca ttatgttaac cggcgtacga acacaagaac tgcgttttgc acgctgggaa
    28141 gatatcgatt ttgagacaaa attgtgggag atcccggcag aagtaatgaa gatgaagcga
    28201 cctcatattg tgccgctctc tgagcaggtc attatgctat ttaagcagct tgaaccaatc
    28261 tcaaaacatc atcccctagt ctttatcggt agaaatgatc cccgcaaacc aattagtaag
    28321 gaaagcatta accaagtaat tgaattactt ggatataagg gaagactcac agggcacggt
    28381 ttcagacaca ccatgagtac aattctgcat gaacaaggct ttaattccgc cttgattgaa
    28441 atgcagttag ctcatgtgga taaaaattcc atcaggggta cctataatca tgccctgtat
    28501 ctcgatggcc gccgtgaaat gatgcaatgg tacgctgatt acattgattc actctccagc
    28561 cgggaaagtt aagtcagaaa tacttttccc tgatgttttc ctcaattaag tattttatcc
    28621 tctggcctac aaggctggag gatttttgtt attatattat ttttttgcgt cttcaaactt
    28681 attacaaaaa cataattaaa aacaaattga catttttaaa ttggcaatta caaaatacag
    28741 ccttgcagtt atttcgatac ctttagcaaa ggaaaagttg attcagatat aatttagcat
    28801 ttcaatcatc tgacatcatt ataaaaaatg agattcacat aaagataaaa ttattgttct
    28861 caaggaaagc atgaaggggc ttcgtaagca atattcattc ggtgccaaga caggacttga
    28921 tacacagaat ctcaactttt atgctctcag actatatcac cacatgtttt acaggctcag
    28981 aaaatgaacg atagattatg ctttgaagtg cacgataaca agggttattt cgtatttccg
    29041 gatacctggt ttggcccatt gctgggggag tttgaagaag tgcttgatgc atacgacgct
    29101 gatgaaatca gcgagacgag ttacatcaac aaactccgac gccttgctca acgggagccg
    29161 gactttattg atatacacgc tcatctggca tatgcatttc tggaacagaa tgccccgcgt
    29221 aaggcactga acgccgctct taaaggactg gcggcaggta atcgtatcat cccggaaagt
    29281 ttctgtggtg aaataatctg gatgcatccc gaaaaccggc cttatctgcg cgcgctttat
    29341 gcagcaattc tcgcaaatgt gcacttacag cgccatcagg atgcggttat gctcactgat
    29401 aaaatactgg catacaatcc tgaagataat cagggggctc gctggttgtt gggttcagag
    29461 ctgttacgca ccggcgatca tgagcgagcc ttcagtgtac tgaaagagca tgctgatgaa
    29521 ttttcacctt actggtatga actcggtctc ctgcattttc tgaacgggga acatgttaag
    29581 gccgccacag cttttcgcca tggctttgcc acaaacactt acatcgcaga aatgctgtgc
    29641 ggaaatctgc atccatttcc acttgctgtg tggcatgact tctccggctc tctggatacg
    29701 gcagaagatt actatgcaac atactcacca ttatggggac aatatccgga agcattgctg
    29761 tttgtaaact ggctctataa ccattcctcc gtgctgcatg aacgatcaga aatcattaaa
    29821 tgcgccgaaa tgctgattca ggaagatgat ttcgaaatat gcgaaagtat tttaagacag
    29881 caggagcacc tgtggaaaag aattgacaag acactttctg aggaaattgt acagaaatgc
    29941 agaaatatga acggtgaata tatctggccc tggatattgc ccttttccgc agcagggatg
    30001 aaacattcaa gaatccagta tcagtagata ttgcattagt gtaactttaa aacaagtaat
    30061 aatttattat ccaataataa cactgtgttc cttccccagg tgattaccct ggggttattt
    30121 tctgaaagtt tcctttgcaa aaggatagcg ttgtaaatta ctttaacgaa ttactccatg
    30181 aactcattaa agttctttcg taatacttta tcgtcaaaca cgttatagct ggcatcaaaa
    30241 accacagatt taccctgcca gcgggtaatc ccctcctctg gcgttttcgg ttgaactggt
    30301 atccccgtcc aacacaggca gccattataa acagtaaaaa attgtattcg ctgttatgaa
    30361 atcaatagtc ctgacgtact gttgaatgtg cttgtgttct gaacctcgta caaccagtac
    30421 atttgatatc gaattataaa caaaacattg catccaactt ttttaatatg gacaatagtt
    30481 gtccatacgg tggttttata ttcgataaat caatggattt cattgagtca caggactggt
    30541 aatggatgaa aatgctttaa ggtttgcctc atactggcgc aactcgcttg cagatgctga
    30601 gtcaggaaag ggctgttttg aacggaaaga cgcccaaaat ttcactcact ggcatgggat
    30661 agcggcggga cgtcttgacg aagcgattgt caataaattt tttaagggag aaaaagacga
    30721 tgtcgaaact gtcaatgtca tcttacgccc aaaagtttat ttccggttac tgcagcatgg
    30781 taaggaccgt tccgcaggca cgcctgatat tgttacaccg atagtgacgc cagccttgct
    30841 aagccgtgag ggttttttat atccgacgcc agcgacctcc attcccagag acctgcttga
    30901 acctttgcca aaaggagcat tttcgattgg tgagattggg cagtatgaca aatacaagac
    30961 gacccatact gatcctaccc acgtaatgtg gacacggccc taagcgagat tatggttttc
    31021 aaattgttcc gggctgagac cgccacaggc actgtgacga cgccagcgat tgtaatcaca
    31081 ctcgatataa ttaaacaccg ttgcccgcat tatttcccgg ctgctaaagc gttccccgtg
    31141 gatacattcc accttcagcg aatgaaagaa gctttccaca caggcattgt cataacagta
    31201 gcctttcgca ctcatactgc cacgcaggtt gtgtcgcttc agcattgcct gataatcccc
    31261 tgaacagtat tgaccaccac ggtccgtatg aacaatgacg ctttccgggc gtcttctccg
    31321 ccacaacgcc atttgcagtg catcacaggc cagttgtgct gtcattcgcg gtgacatcga
    31381 ccagccaatg acggcgcgtg accacaggtc gatgactact gcgagataca accagccctc
    31441 atcggtacgc aagtacgtga tgtcacccgc ccacttctgg tttcgggcca ctggcgctga
    31501 agtcctgctc cagcagattc tccaatacgg gcaggccatg tgcacggtag ctgaccgggc
    31561 tgaacttccg gctggctttc gcccgcagcc cctgacgacg caggctggcg gcaatggttt
    31621 taatattgaa ctccggcagt tcgtcagcaa ggcggggagc accgtatcgc tgctttgcct
    31681 caaagaatgc cttatggaca gcggcatcgc aggtgagccg aaactgttgg cgcaggctca
    31741 tctggtgacg acgcctgagc cagacatacc agccgctgcg ggcaacccga agtacacgac
    31801 acatcgcttt gatgctgaac tctgcccgat gattttcgat aaagacatac ttcatttcag
    31861 gcgcttcgcg aagtatgtcg cggccttttg gaggatggac agttcctcag cctgctccgc
    31921 cagttgtcgt ttaaggcgga cattttcagc ggccagttcg ctttcgcgct ctgacgaact
    31981 catttgttgc tgctgtttac tgcgccaggc ataaagctga gattcataca ggctgagttc
    32041 acgggctgcg gcggccacac cgatgcgttc agcgagtttc agggcttcgt tacgaaattc
    32101 aggcgtatgt tgtttacggg gcttcttgct gattgatact ggttttgtca tgagtcacct
    32161 ctggttgaga gtttactcac ttagtcctgt gtccactatt ggtgggtaag atcaccctcc
    32221 attaaaacta aaacgggcgc tttctgatat atcat
//
LOCUS       DAJTRQ010000031        31869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000031 DAJTRQ010000000
VERSION     DAJTRQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO332_001567"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO332_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5942087.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO332_001568"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO332_001568"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5942088.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO332_001569"
     CDS             complement(3736..4092)
                     /locus_tag="OO332_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5942089.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO332_001570"
     CDS             complement(4096..4512)
                     /locus_tag="OO332_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5942090.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO332_001571"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO332_001571"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5942091.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="OO332_001572"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="OO332_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="OO332_001573"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="OO332_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5942092.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="OO332_001574"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="OO332_001574"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5942093.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="OO332_001575"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="OO332_001575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5942094.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="OO332_001576"
     CDS             10540..11523
                     /locus_tag="OO332_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5942095.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="OO332_001577"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="OO332_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5942096.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="OO332_001578"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="OO332_001578"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5942097.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14450)
                     /locus_tag="OO332_001579"
                     /pseudo
     CDS             complement(13465..14450)
                     /locus_tag="OO332_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14768..15283
                     /gene="zur"
                     /locus_tag="OO332_001580"
     CDS             14768..15283
                     /gene="zur"
                     /locus_tag="OO332_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5942098.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15325..15534)
                     /locus_tag="OO332_001581"
     CDS             complement(15325..15534)
                     /locus_tag="OO332_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5942099.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15650..16975)
                     /gene="dinF"
                     /locus_tag="OO332_001582"
     CDS             complement(15650..16975)
                     /gene="dinF"
                     /locus_tag="OO332_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5942100.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="OO332_001583"
     CDS             complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="OO332_001583"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5942101.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17766..18134)
                     /gene="dgkA"
                     /locus_tag="OO332_001584"
     CDS             complement(17766..18134)
                     /gene="dgkA"
                     /locus_tag="OO332_001584"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5942102.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18305..20728
                     /gene="plsB"
                     /locus_tag="OO332_001585"
     CDS             18305..20728
                     /gene="plsB"
                     /locus_tag="OO332_001585"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5942103.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="OO332_001586"
     CDS             complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="OO332_001586"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5942104.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="OO332_001587"
     CDS             complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="OO332_001587"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5942105.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22488..24068)
                     /locus_tag="OO332_001588"
                     /pseudo
     CDS             complement(22488..24068)
                     /locus_tag="OO332_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24297..25217)
                     /gene="malM"
                     /locus_tag="OO332_001589"
     CDS             complement(24297..25217)
                     /gene="malM"
                     /locus_tag="OO332_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5942106.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="OO332_001590"
     CDS             complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="OO332_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5942107.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26781..27896)
                     /gene="malK"
                     /locus_tag="OO332_001591"
     CDS             complement(26781..27896)
                     /gene="malK"
                     /locus_tag="OO332_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5942108.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28261..29451
                     /gene="malE"
                     /locus_tag="OO332_001592"
     CDS             28261..29451
                     /gene="malE"
                     /locus_tag="OO332_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5942109.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29605..31149
                     /gene="malF"
                     /locus_tag="OO332_001593"
     CDS             29605..31149
                     /gene="malF"
                     /locus_tag="OO332_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5942110.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31164..>31869
                     /gene="malG"
                     /locus_tag="OO332_001594"
     CDS             31164..>31869
                     /gene="malG"
                     /locus_tag="OO332_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5942111.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASL"
BASE COUNT         8124 a         8177 c         7710 g         7858 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaacttatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa cctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt tttacgtgca
    14221 tgccataaat tatgcgtgat tcgttgaata aacaaatcta tttttgcaat aattatatcc
    14281 cgatgacgca gcgctgcata atcaatagat ttaccatata tattatttaa tttacctttg
    14341 attgaacctc tattatccaa tttatctttg gttgcatctt tattttcgaa ttgatttgaa
    14401 ttgagtgttt tattaaagta tccagttgca ccgggtatta tcttcaacat gattcatcta
    14461 ccttttttgt cgtaccgtgt cctccttata attcttttgc catctggtca ctatcaaaaa
    14521 tcgctcataa tttctgaact acaggtatgc gtttaaaccc agttaaataa atagcgagtt
    14581 aacttgaagc aatttattta aaaatgaatt attaattatt atcgtgttga taatggtacg
    14641 acaaggcaac acaacacctt acctacaaaa cagaaaatca acattacggc aacaataagg
    14701 gttctcggtg tttttcctgg aacatggtaa agtaaggaca ttcttaaccc ccactttgag
    14761 gtgcccgatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    14821 gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg ttgcgcctga tgagtctgca
    14881 ggatggcgct atcagcgctt atgatctgct tgatttactg cgcgaagctg aaccgcaagc
    14941 caagccgcca acggtttatc gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa
    15001 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gaccagccca cgcatacgtc
    15061 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga
    15121 agacattatg catacgctgg cggcaaaaat ggggtttgcc ctgcggcata atgtgattga
    15181 agcacatggg ctctgtgcgg catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg
    15241 ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg
    15301 tacacgtcgg gcaggaggga ttaattacca gcgatattca ttgcgggttt cccaatccac
    15361 gacctctttt tctgcctgat ctttctgata accataacgt tcctggattt taccgaccag
    15421 ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    15481 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    15541 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    15601 gtgtagtcag caatttggct taggataatt tattcagaat ttttaaccgt cacgttgcgg
    15661 caaaccaggt accgttgcgc cagtgacgcc gccagatagc cgccagagaa agcccgcgca
    15721 acgccagaaa gacggttaat gccagccaca aaccatgatt acccagccac ggcagcgtaa
    15781 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    15841 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccaacc aacggcaaaa
    15901 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    15961 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacaatcc
    16021 ccgactggcg gcacgctgcc cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt
    16081 atgcctgacc ggagtgcgct tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa
    16141 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcac
    16201 caagtacggt gatcgcgccg aaacagagtt gcaacaacag cgaacgcagc atgatatcgc
    16261 ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc agttttcagc atttcgccgg
    16321 aaattccgcg tagtttgagg attttacgca ccattagcag accaatcagc aatgttgcat
    16381 attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt catatgcagc cccatcacca
    16441 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    16501 gggcatattg cacgccgagt aaccaaccga gtaataccag attcgccagc gacgccggag
    16561 cgcttaacca gcggatttca agaaagcgcc gcgcctgttc caggaccgct tcactaccgc
    16621 caacaatatg cagcgccaga tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg
    16681 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttgg
    16741 caccataagc ctgcgcagtc agcccggtgg tgctcatgcg taaaaacagc aacagcataa
    16801 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    16861 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    16921 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    16981 caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag
    17041 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    17101 agctctgctg acgaaggtca acgacaattg gtttaaactc gctattttct ggcaacagtt
    17161 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    17221 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcgccatcca
    17281 taatgccgat atctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
    17341 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    17401 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
    17461 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    17521 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    17581 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    17641 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatactgtg agtatataca
    17701 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    17761 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    17821 gcaatcagca ccgcggcgga tcccatatct tttgcgcgtc cggaaagctc atggtattca
    17881 gagccaattc ggtcaaccac tgcttcgatg gcgctattga ggatttccac aatcatcacc
    17941 agcatcacgg agctgataag cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg
    18001 atgaccaccg ccaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    18061 gcagcgcgta aacctttcca ggaatagcca gcagctttga taattcgggt gaatccagtg
    18121 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    18181 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    18241 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    18301 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    18361 ggtaaaaagc aagtctattc cggcagatcc tgccccggaa ctggggctgg atacctctcg
    18421 tccaattatg tacgttttac cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca
    18481 gtgtctggca catgatttgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    18541 gccgcgctat gtgttcattc acggcgggcc gcgtgtattc acctattaca cgccgaaaga
    18601 agagtctatt aagcttttcc acgactatct cgatttgcac cgtagcaacc caaatctgga
    18661 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    18721 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    18781 cggtcgcgac agttttgtgc gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga
    18841 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc
    18901 ccgtcaacgt ctggctgccg taggcccacg tctgcctgct cgtcaggatc tgtttaataa
    18961 gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat
    19021 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    19081 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgatttta
    19141 tcagggcatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggtcatga
    19201 gctggtgtat gtgccttgcc accgcagtca catggactac ctgctacttt cttacgtgct
    19261 gtaccaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    19321 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtactt ttaaaggcaa
    19381 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    19441 cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac
    19501 cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct
    19561 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    19621 gcgcggcgcg acgaaagaga aagagagcct gccgcagatg ctgcgcggtt taagcaagct
    19681 gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca atgccgttga tgacctacct
    19741 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    19801 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    19861 aggcgcggca aacgccatga acctgtgctg tactgcgcta ctggcatcac gtcagcgctc
    19921 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    19981 accttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    20041 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    20101 acgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    20161 gccttcgctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    20221 ggagcacgtc aatgtgcttt acccaatgct gaaagcggag ctgttcctgc gctgggatcg
    20281 cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    20341 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcaccc tacagctgct
    20401 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggt tgttgagtgc
    20461 caacccgtcg atcaaccgcg gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct
    20521 ctccgtgctg cacggcatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    20581 ggtgctgacg ctgcgtgatg aagggtatat cagcgatagc ggcgatgccg aaccagcaga
    20641 aacgatgaag gtttatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    20701 tgagagtgcg acgcagggcg aagggtaatc agagagaatt gccggatgcg gcgaaaacgc
    20761 cttatccggc ctaccatgac ctgcaaattc aataaattgc gattcaccgg gtaggcctga
    20821 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    20881 catcagaaat gccagtaact cattgccagt cctaaaaaca gtaccagacc aacatagtta
    20941 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    21001 taaacaaaca gcgcgccagc caccacaatt gaccagtaat atccccagcc taagccattt
    21061 aactcaccga tgatcgccat cagtgccagt acgccaatct gcaaaatacc aataatcaat
    21121 ttatcgtatt ggccgaacag gattgccgtg gatttaatgc caatcttcac atcatcatcg
    21181 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    21241 attaaccagc aactcaatgg caccgactca ctcacagcgg caaaagccat tggaatcgac
    21301 cagccaaacg ccgcgcccag caccacttgc gggagatggg tataccgctt cataaacggg
    21361 tacacccacg ccaatgccag agcggcaatc gacaacagaa tggtcatcgt attcagcgtc
    21421 agaaccagta aaaacgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctct
    21481 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    21541 cgatcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac acccgcgaca
    21601 aacaccgcca ggatccagag ctgaggaacg cccggtgtcg ccacccacaa cgcccataat
    21661 gttggccaga gcagcaataa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    21721 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    21781 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    21841 cgtcgccccc acagtccggc atcacggcct atctcgataa aatcccgggt tagtgtcgat
    21901 gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt tttgtaacgc tagttccggc
    21961 ccgcttaatg ttgatacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    22021 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagtagcgg cagttcttcg
    22081 gggatttcat tctgctcgac aaacccctcg cggatcatcg tcacactcac cgtttttccc
    22141 tgctgttcaa aacgttttgt catggaatcc tccagcaaca gccagtcgaa cagttgcgga
    22201 tccagggcag ggatctcttt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    22261 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa agtgtttacg
    22321 ggagatgtaa agcaaattaa gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag
    22381 tggtaaaacg ctgttgaacc ttacgctttg ccgatgcccg tgctggcgag ggcaaccata
    22441 ggggcatcac cgcccatgat gctaggaacg gtgatgtttt cctttactta aataccttta
    22501 ttctttatac gagtagctcg ccaactcgca gtaaaaattg ggaaaagcgc atatttacca
    22561 tgagatttgg agctgaaaag tgtttcctga gtaagattaa taggcaacat cttatgtctg
    22621 gatttataga tacacgtgga attccaattg gtatcttttg taggattcaa catatcatgt
    22681 aagcgctggg agcttaccac cataacaggc ccatcatcga cagaggagaa caggataaat
    22741 atattatcgt ttcgtgttaa tttattagtg ttaaaggttc ttaacttttc ttcagtttct
    22801 ataaatatag gtgaaacatc atcactacga atccactctt tataaagata ccaggcattt
    22861 gctttgatca tatcatccgt tgatcgtaag gcctgatcga taagttggat aaaaaaattg
    22921 ttatttttat caaccagccc tttattcata ttaaatctgt taataaaatg gatcatctgt
    22981 tgggtatcga aattcacctt actgaatcga tgttgacgca taaaattatc gaacttgtca
    23041 tcttccccag aatgtatata actacgtata attggatctt ttatataagg acaatggcta
    23101 aatgttttct tcatattctc aataaagttt ttattaacaa tattgagaat ttttaattgc
    23161 atcaccagtt ttactgccag aacggatttc aatatcattg cattatctgg caatgtatca
    23221 atcattttta taatgttatt gatataacca tcgagatctt cattcccaaa tatatctgaa
    23281 taatcaatag ttaattccaa ttgttctgta atgcaatctt aaaaattccc tgtcgacatt
    23341 cgcacatgat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
    23401 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
    23461 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
    23521 gtatcaagaa tattttggct aaaatcaaca cattcgccac tcgttttgag aaaaatcaca
    23581 ttgtgtaaac gtcgacattt aaatatgaca ttatgaactg ttgccttagt aaaattgcaa
    23641 ttctcaattt ctttacattc gaaagttaca tgttgtagat ttgatgataa aaaagacacc
    23701 ttagaaataa cgtcaccttt aaaatgacaa tttgttaaat caaaatcaga caggtcgata
    23761 tacccctgcg catctttatc catcagaaaa ttacaagcgc attctatttt ttcatttctg
    23821 tagaatttta cacttatagt atcattacta attgagactt cacgtaacat atttattgca
    23881 tgattttttt cgttccgttg ttttatattt aaaaatatat cactgatata aataaagtta
    23941 tctctgttta aacctgtcac cccattgaaa ttacgtagta aatcaagatc tactatatta
    24001 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt tgtgatatga
    24061 cctatcataa aacctctgta atacagaaat aaaataacct ggaaaagtag cctgagcgtc
    24121 cagacactac cggcgaaagg aaaagaatct ctgataatgc attgaaataa tggacattct
    24181 tagtgaagaa gaagtgaagt actataaaaa atcgctcata tcccaggaag gaagcataaa
    24241 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    24301 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
    24361 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    24421 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggcacc
    24481 ggagcaggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    24541 gaagaaccaa atagtggtcc taccaacacg ctggagctgg agttcgtttt gactttcagt
    24601 ttcagtaagc catcgctggt atgacgagca accggatcgg gaatatccgg gatcgagtta
    24661 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
    24721 ttttccatgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    24781 ataacgcctt ccagccgatc tgcactcatc acgcctagtt cctggtaggt gaaataactg
    24841 ctggggaaga aggctgatgg ggtcatgttc tgatcaataa tcagcacgtt cggcgcaaaa
    24901 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
    24961 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgctcagttg ttggccgccg
    25021 gtcgccagtt gggtggtctg ggttttagat tgatcgaccg gtgtccaggt gagttgttgc
    25081 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    25141 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag ggcgatgaga
    25201 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    25261 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
    25321 tttatgccgg atgcggcgta aacgccttat ccggcccagg ttttgctatt accaccagat
    25381 ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt
    25441 gaaatcagca ggaacggctt tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc
    25501 gtaaccccat ttctcatccc acttggcgta ggttgcgaag acacgaatag ccgggcgtga
    25561 ccagatgctg tcgccagcct gccattgttg tgcgagggta attttgtact gattgttctt
    25621 gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt tccatcacgg tgctcatgat
    25681 tggcgtccac ttgtacatcg ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt
    25741 atcccagttg atatcctggt acatacccac gtacatcatg tcccagttgt cgcccatgga
    25801 gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa
    25861 tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga cctttaccct gcgaggtcat
    25921 cgagtcagta gcgtactgaa caacaaactt gttaaagccc ttcaggacac tctgagtatg
    25981 ttcagcagtg aataaccagc cgtctttcga tgcgccatca accagacgat agttatcacg
    26041 caggttggca cgaccgtagt cgacacccag ttctaatgtg ccgcccgggt tgatttccat
    26101 ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt
    26161 gttgctggca aaagaggaag aaccaccagc ttcagaggag cgggttgctg ccagagagag
    26221 tttaccgaag ccaacatcga tgttttccag accggcacca ggaccagaaa tatcccagta
    26281 gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag cgcttacctg cccagatggt
    26341 ggaacctggc agccattcga tcaggttttt tccctgcacg tttgcttcac ggaaggccgg
    26401 atcggtagct tcccagtcat tctgttgcgc gacggaatag gccacgttag tgtcgaaata
    26461 gaagctctta tcgccctctt tccacacttc ctgacccaat tttaattcag cataagtttc
    26521 acattcgttg ccaagacggt atttactttg agcaccggta gtctggaaac actgttgttc
    26581 accgccgcta cctgtccagc caataccgga acgtgcatag ccgtggaaat caacagccat
    26641 tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt
    26701 aatcatcatt ctatctcctg agtcattgct tttctttttt cacatcacct gtgacaggct
    26761 ttgtgtgttt tgtggggtgc ttaaacaccc ggctccttat gcagtcgacg acatgcagtg
    26821 ccatcctcac ggaacagatg gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct
    26881 tcttctacca acaccacgtc gttctggcgg tacaccaggt tttgacgaat ggaagggatc
    26941 tggatataga tttgggtttc gttgccgagt tgctcgacga cctgaacttc accctcaagg
    27001 atgacgtcag cgatatcact cggcagtaga tgttccgggc gaatacccag cgacatattg
    27061 gctccaacct ggacatcacg gctttcaaca ggcagccaga cttgttgacg atttggcatc
    27121 ggcagctcca cctgcacttg atcgattgcg gtggcggtca cttttaccgg caggaagttc
    27181 atctttggcg aaccgataaa tccggcgaca aaacggtctg ccggatagtg gtacagttcc
    27241 agcggtttcc caacctgtgc cacgcgaccg gcgtccagca ccacgatttt gtcggccagc
    27301 gtcatcgctt cgacctgatc gtgggtgacg taaatcattg tgcggcccag gcgtttatgc
    27361 agacgggaga tttcgatacg catttgcaca cgcagtgcag catcgaggtt ggagagcggt
    27421 tcatcgagca aaaatacgct tggctcggcc accagcgtac ggccaatcgc cacgcgctga
    27481 cgctgaccac cggagagcgc tttcggtttg cgatccagca aatgcgccag ttgcagcact
    27541 tccgccacct ggttgacgcg ttggttaatc acctcttttt ttgcgccagc cagtttcagg
    27601 ccaaatgaca tgttttctgc tactgacagg tggggataga gcgcgtaaga ctgaaacacc
    27661 ataccaacgc cgcgttctgc tggcggagtg tcattcatcc gtttctcacc gatgaacagg
    27721 tcgccgctgg tgatcgtctc aagcccggca atcatgcgca gtaaagtcga tttaccgcag
    27781 ccagacggtc cgacaaacac cacgaattca ccttcatgga tatcgagatt gatatctttc
    27841 gataccacga cctcgcccca ggctttcgtt acattttgca gctgtacgct cgccatgccc
    27901 ttctcccttt gtaacaacct gtcatcgaca gcaacattca tgatgggctg actatgcgtc
    27961 atcaggagat ggcttaaatc ctccaccccc tggctttttt atgggggagg aggcgggagg
    28021 atgagaacgc ggcttctgtg aactaaaccg aggtcatgta aggaatttcg tgatgttgct
    28081 tgcaaaaatc gtggcgattt tatgtgcgca tccccacatt accgccaatt ctgtaacaga
    28141 gatcacacaa agcaacggtg gggcgtaggg gcaaggagga tggaaagagg ttgccgtata
    28201 aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt
    28261 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
    28321 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
    28381 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
    28441 gtcaccgttg agcatccgga taaactggaa gagaaattcc ctcaggttgc ggcaactggc
    28501 gatggccctg acattatctt ctgggcgcac gaccgctttg gtggctacgc tcaatctggc
    28561 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacatgg
    28621 gatgccgtac gttacaacgg caagctgatt gcttacccaa tcgctgttga agcgttatcg
    28681 ctgatttata acaaagacct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
    28741 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
    28801 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
    28861 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
    28921 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
    28981 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
    29041 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
    29101 tctaaaccgt tcgttggcgt gctgagcacc ggtattaacg ccgccagtcc gaacaaagag
    29161 ctggcgaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
    29221 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
    29281 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
    29341 cagatgtccg ctttctggta tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt
    29401 cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta atgctgtgaa
    29461 atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg
    29521 ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga taaggcgtga aagccttatc
    29581 cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa agaaacattg gtggcaaagc
    29641 gacgcgctga aatggtcagt gccaggtctg ctcggcctgc tggtgggtta ccttgttgtt
    29701 ttaatgtacg cacaagggga atacctgttc gccattacca cgctgatatt gagttcagcg
    29761 gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc gctatgttta cccgggaatg
    29821 gctggaatgg gattattcgt cctcttccct ctggtctgca ccatcgccat tgccttcacc
    29881 aactacagca gcactaacca gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc
    29941 tcctggcaag caggcaaaac ctataacttt ggtctttacc cggcgggcga tgagtggcaa
    30001 ctggcgctca gcgacggcga aaccggcaaa aattacctct ccgacgcgtt taaatttggc
    30061 ggcgagcaaa aactgcaact gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat
    30121 ctgcgcgtga ttacccagaa tcgtcaggcg ctgagtgaca ttaccaccat tctgccggat
    30181 ggcaacaaag tgatgatgag ctccctgcgc cagttttctg gcacgcagcc gctctacaca
    30241 ctcgacggtg acggcacgtt gacgaataat cagagtggcg tgaaatatcg tccgaataac
    30301 caaattggct tttaccagtc cattaccgcc gacggcaact ggggggatga aaagctaagc
    30361 cccggttaca ccgtgaccac cggctggaaa aactttaccc gcgtctttac cgacgaaggc
    30421 attcagaaac cgttcctcgc cattttcgtc tggaccgtgg tgttctcgct gatcactgtc
    30481 tttttaacgg tggcggtcgg catggttctg gcatgtctgg tgcagtggga agcgttgcgc
    30541 ggcaaagcgg tctatcgcgt cctgctgatt ctgccctacg cggtgccatc gttcatttca
    30601 atcttgattt tcaaaggatt gtttaaccag agcttcggtg aaatcaacat gatgttgagc
    30661 gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga ccaccgctcg cacgatgctg
    30721 attatcgtca atacctggct gggttatccg tacatgatga tcctctgcat gggcttgctg
    30781 aaagcgattc cggacgattt gtatgaagcc tcagcaatgg atggcgcagg tccgttccag
    30841 aacttcttta agattacgct gccgctgctg attaaaccgc tgacgccgct gatgatcgcc
    30901 agcttcgcct ttaactttaa caacttcgtg ctgattcagc tgttaaccaa cggcggcccg
    30961 gatcgtcttg gcacgaccac gccagccggt tataccgacc tgcttgttaa ctacacctac
    31021 cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc tggcggcagc aattgccacg
    31081 ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc tgaaagccac gcgaatgaag
    31141 tttgattaag ggagataaca aaaatggcaa tggtccaacc gaaatcgcaa aaagcacgtt
    31201 tgtttattac tcacctgcta ctgctacttt ttatcgcggc gattatgttc ccgctgctga
    31261 tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac cggtagcctg atcccggagc
    31321 aaatctcctg ggatcactgg aaactggcgt taggttttag cgttgaacag gctgatggtc
    31381 gcattacgcc accgccattc ccggtactgc tgtggctgtg gaactcggta aaggtcgctg
    31441 ggatttccgc gattggcatt gtggcgctct ccaccacctg cgcctacgct ttcgcccgta
    31501 tgcgctttcc aggcaaagcg acgctgctga aaggaatgct gattttccag atgttcccgg
    31561 cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg tctgggtgag tacattccat
    31621 tcattggcct gaatactcac ggcggcgtaa ttttcgcgta tctgggtggg attgcgctgc
    31681 atgtctggac catcaaaggc tatttcgaaa ccatcgacag ttcgctggaa gaagcggctg
    31741 cgctggatgg tgcgacaccc tggcaggcct tccgccttgt cctgttgccg ctgtcagtac
    31801 cgattctggc ggtggtattc atcctgtcgt ttatcgctgc cattactgaa gttccggtcg
    31861 cgtcgctgt
//
LOCUS       DAJTRQ010000032        31799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000032 DAJTRQ010000000
VERSION     DAJTRQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..329)
                     /locus_tag="OO332_001595"
     CDS             complement(<1..329)
                     /locus_tag="OO332_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR5942112.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYL"
     gene            complement(507..1418)
                     /gene="gsiD"
                     /locus_tag="OO332_001596"
     CDS             complement(507..1418)
                     /gene="gsiD"
                     /locus_tag="OO332_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5942113.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1421..2341)
                     /gene="gsiC"
                     /locus_tag="OO332_001597"
     CDS             complement(1421..2341)
                     /gene="gsiC"
                     /locus_tag="OO332_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5942114.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2359..3897)
                     /gene="gsiB"
                     /locus_tag="OO332_001598"
     CDS             complement(2359..3897)
                     /gene="gsiB"
                     /locus_tag="OO332_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5942115.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(3917..5788)
                     /gene="gsiA"
                     /locus_tag="OO332_001599"
     CDS             complement(3917..5788)
                     /gene="gsiA"
                     /locus_tag="OO332_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5942116.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5775..6740)
                     /gene="iaaA"
                     /locus_tag="OO332_001600"
     CDS             complement(5775..6740)
                     /gene="iaaA"
                     /locus_tag="OO332_001600"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5942117.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTSDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6944..8179
                     /gene="moeA"
                     /locus_tag="OO332_001601"
     CDS             6944..8179
                     /gene="moeA"
                     /locus_tag="OO332_001601"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5942118.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8179..8928
                     /gene="moeB"
                     /locus_tag="OO332_001602"
     CDS             8179..8928
                     /gene="moeB"
                     /locus_tag="OO332_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5942119.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9107..9769)
                     /gene="fsa"
                     /locus_tag="OO332_001603"
     CDS             complement(9107..9769)
                     /gene="fsa"
                     /locus_tag="OO332_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5942120.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9900..10799
                     /locus_tag="OO332_001604"
     CDS             9900..10799
                     /locus_tag="OO332_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5942121.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10805..13237
                     /locus_tag="OO332_001605"
     CDS             10805..13237
                     /locus_tag="OO332_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5942122.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13383..14198
                     /gene="ybiV"
                     /locus_tag="OO332_001606"
     CDS             13383..14198
                     /gene="ybiV"
                     /locus_tag="OO332_001606"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5942123.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14350..15615
                     /locus_tag="OO332_001607"
     CDS             14350..15615
                     /locus_tag="OO332_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5942124.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15856..17448)
                     /locus_tag="OO332_001608"
     CDS             complement(15856..17448)
                     /locus_tag="OO332_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5942125.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            17667..18587
                     /gene="ldtB"
                     /locus_tag="OO332_001609"
     CDS             17667..18587
                     /gene="ldtB"
                     /locus_tag="OO332_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5942126.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18645..19763)
                     /locus_tag="OO332_001610"
     CDS             complement(18645..19763)
                     /locus_tag="OO332_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5942127.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19760..20227)
                     /gene="mntR"
                     /locus_tag="OO332_001611"
     CDS             complement(19760..20227)
                     /gene="mntR"
                     /locus_tag="OO332_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5942128.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20413..20541
                     /gene="mntS"
                     /locus_tag="OO332_001612"
     CDS             20413..20541
                     /gene="mntS"
                     /locus_tag="OO332_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5942129.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20813..22396
                     /gene="opgE"
                     /locus_tag="OO332_001613"
     CDS             20813..22396
                     /gene="opgE"
                     /locus_tag="OO332_001613"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5942130.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22444..22959)
                     /gene="ompX"
                     /locus_tag="OO332_001614"
     CDS             complement(22444..22959)
                     /gene="ompX"
                     /locus_tag="OO332_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5942131.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23012..23077)
                     /locus_tag="OO332_001615"
     CDS             complement(23012..23077)
                     /locus_tag="OO332_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942132.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23312..24199
                     /gene="rhtA"
                     /locus_tag="OO332_001616"
     CDS             23312..24199
                     /gene="rhtA"
                     /locus_tag="OO332_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5942133.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24498..25001
                     /gene="dps"
                     /locus_tag="OO332_001617"
                     /gene_synonym="pexB"
     CDS             24498..25001
                     /gene="dps"
                     /locus_tag="OO332_001617"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5942134.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25405..26151
                     /gene="glnH"
                     /locus_tag="OO332_001618"
     CDS             25405..26151
                     /gene="glnH"
                     /locus_tag="OO332_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5942135.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26290..26949
                     /gene="glnP"
                     /locus_tag="OO332_001619"
     CDS             26290..26949
                     /gene="glnP"
                     /locus_tag="OO332_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5942136.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26946..27668
                     /gene="glnQ"
                     /locus_tag="OO332_001620"
     CDS             26946..27668
                     /gene="glnQ"
                     /locus_tag="OO332_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5942137.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            27929..30153
                     /gene="ybiO"
                     /locus_tag="OO332_001621"
                     /pseudo
     CDS             27929..30153
                     /gene="ybiO"
                     /locus_tag="OO332_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(30150..31076)
                     /gene="rlmF"
                     /locus_tag="OO332_001622"
     CDS             complement(30150..31076)
                     /gene="rlmF"
                     /locus_tag="OO332_001622"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5942138.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31352..31612
                     /locus_tag="OO332_001623"
     CDS             31352..31612
                     /locus_tag="OO332_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5942139.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
BASE COUNT         7755 a         8517 c         7914 g         7613 t
ORIGIN      
        1 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
       61 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      121 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      181 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      241 atcatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      301 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa
      361 cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
      421 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
      481 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
      541 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
      601 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac
      661 tctggtgtcg gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc
      721 gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata
      781 tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
      841 tgctgtttca acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa
      901 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
      961 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1021 caccagcctt cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata
     1081 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1141 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1201 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1261 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1321 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1381 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1441 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     1501 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta
     1561 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa
     1621 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     1681 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     1741 ttaaaacgtc gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc
     1801 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc
     1861 agcccagttc aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg
     1921 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg
     1981 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2041 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     2101 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2161 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     2221 gcccggcaat caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca
     2281 ccgagacgat aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca
     2341 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg
     2401 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     2461 cgggatccac ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt
     2521 ttccgccgga tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     2581 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag
     2641 tgcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     2701 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac
     2761 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     2821 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     2881 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     2941 ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt
     3001 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3061 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3121 ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa
     3181 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3241 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3301 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3361 accaatctcc ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg
     3421 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     3481 atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     3541 cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc
     3601 ctggaattta attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     3661 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     3721 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt
     3781 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     3841 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     3901 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     3961 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4021 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4081 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4141 cgcaccgtga accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4201 tccgctctac cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4261 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg
     4321 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4381 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4441 cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta
     4501 cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     4561 ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg
     4621 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     4681 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     4741 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     4801 acaaaccgct gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc
     4861 catccaccac cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata
     4921 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     4981 ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5041 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5101 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5161 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     5221 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5281 agagttgatg cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     5341 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5401 attcggcaat ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa
     5461 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     5521 gttcaatcac atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc
     5581 ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     5641 cgcctacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     5701 ctattttctg ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac
     5761 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     5821 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     5881 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     5941 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6001 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa
     6061 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact
     6121 cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct
     6181 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6241 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa
     6301 ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6361 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6421 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     6481 atcacacagg cgtccagttc atgggtttca tcactcgtaa agacagcgcc aattccggcg
     6541 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     6601 tcgccggctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     6661 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     6721 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     6781 aatatcagaa tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc
     6841 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg
     6901 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     6961 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac
     7021 ccaggaaacg ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7081 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     7141 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata
     7201 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     7261 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7321 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7381 tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc
     7441 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     7501 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg
     7561 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     7621 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     7681 agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     7741 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt
     7801 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccgattt cagcgacgct
     7861 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     7921 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     7981 tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac
     8041 cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct
     8101 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc
     8161 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     8221 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     8281 ctggtagtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     8341 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     8401 acactgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg
     8461 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa
     8521 cttgcagcat tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     8581 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     8641 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     8701 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     8761 ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     8821 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     8881 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     8941 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9001 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9061 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9121 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat
     9181 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta
     9241 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9301 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9361 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9421 cttgtgccgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
     9481 taatcgctgc cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag
     9541 aacgcagctt aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
     9601 acagacgccc ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt
     9661 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
     9721 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
     9781 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
     9841 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
     9901 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
     9961 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta
    10021 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
    10081 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10141 ccccggagca tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg
    10201 gtgaagtgaa aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg
    10261 atcgcagcgg cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg
    10321 cgacggcgct gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc
    10381 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10441 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    10501 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    10561 tgattcaggg ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg
    10621 acgagctgca cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat
    10681 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc
    10741 tcgattttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    10801 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    10861 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    10921 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    10981 cctggcgaat cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag
    11041 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11101 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt
    11161 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat
    11221 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat
    11281 gacctccggc gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga
    11341 tggtctgcgt gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga
    11401 tttgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    11461 cattgaacgt ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg
    11521 tcgcgatgaa ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca
    11581 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc
    11641 taacggtcac tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg
    11701 cgacgttgaa ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg
    11761 ctggttgaaa ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc
    11821 gggaagtccg ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc
    11881 aatggacgcg gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc
    11941 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc
    12001 ctgcgtacag gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt
    12061 gatcccggaa tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat
    12121 tggttgtata gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    12181 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    12241 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga
    12301 agtgatggcc gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga
    12361 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12421 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    12481 ttctggcctg caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    12541 ggtgtttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    12601 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    12661 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    12721 actgaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    12781 gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga
    12841 cgggcgtaaa gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca
    12901 tctcggccct actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg
    12961 cggcgtgttg ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca
    13021 gaaactgatg atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta
    13081 caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga
    13141 tctggtagtg cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca
    13201 ggacgatatc atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt
    13261 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13321 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    13381 ctatgagcgt aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa
    13441 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    13501 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    13561 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt
    13621 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    13681 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    13741 aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    13801 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt
    13861 tagtgatcga caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt
    13921 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    13981 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    14041 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    14101 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    14161 aggctgtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc
    14221 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    14281 cttatttata acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc
    14341 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14401 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat
    14461 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    14521 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    14581 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    14641 caagcgctag gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag
    14701 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    14761 taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat
    14821 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    14881 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc
    14941 aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat
    15001 cagcacgttt tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg
    15061 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    15121 cggacattcc agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac
    15181 gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    15241 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg
    15301 catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc
    15361 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15421 aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag
    15481 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    15541 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    15601 ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg
    15661 acgttactca gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt
    15721 atccggccta caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag
    15781 cgtagcgcat caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg
    15841 gtgacggata atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct
    15901 aaagtcgatc acgcattccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa
    15961 ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag
    16021 tgactcgatg gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg
    16081 cttctgcatc attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt
    16141 agctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc
    16201 ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt
    16261 ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt
    16321 tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc
    16381 gacgccgttg gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt
    16441 tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg
    16501 gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac
    16561 ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga
    16621 ggcgttggcg ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt
    16681 ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta
    16741 gttacccgga taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat
    16801 gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac
    16861 ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag
    16921 aatatccggc tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc
    16981 aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc
    17041 accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc
    17101 cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg
    17161 cttcacttcc cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc
    17221 aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt
    17281 acccagcgtc ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc
    17341 gttcgcgcca atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa
    17401 caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg
    17461 aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg
    17521 cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac
    17581 tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc
    17641 ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg
    17701 ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg
    17761 acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc
    17821 cgctggagta ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc
    17881 cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga
    17941 tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt
    18001 actatccgaa agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca
    18061 aagatacgcc tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga
    18121 cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg
    18181 ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg
    18241 ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc
    18301 gtctacgtaa cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac
    18361 agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag
    18421 tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta
    18481 ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg
    18541 atgaagcgat caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta
    18601 aaacaaaggc ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc
    18661 ataactaaca aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga
    18721 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
    18781 gatcctggta ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga
    18841 ggtacataat tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg
    18901 actgccgtta accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc
    18961 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
    19021 aacgtccagt ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca
    19081 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc
    19141 cccagacaat tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat
    19201 tgcagcgcct ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg
    19261 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
    19321 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
    19381 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
    19441 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
    19501 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
    19561 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
    19621 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
    19681 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
    19741 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
    19801 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
    19861 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
    19921 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
    19981 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
    20041 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
    20101 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
    20161 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
    20221 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
    20281 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat
    20341 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
    20401 tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt
    20461 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
    20521 gataaacctt cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca
    20581 ggcataacgc gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc
    20641 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
    20701 tgcgtatatt gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt
    20761 attgaccctt gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt
    20821 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
    20881 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
    20941 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
    21001 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
    21061 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
    21121 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
    21181 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
    21241 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
    21301 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
    21361 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
    21421 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat
    21481 cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
    21541 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
    21601 gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
    21661 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
    21721 ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta
    21781 cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga
    21841 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct
    21901 gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
    21961 atcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
    22021 gctgtacgat cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca
    22081 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata
    22141 tcagcaaaat ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt
    22201 gattaaagcc cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat
    22261 taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat
    22321 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
    22381 cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag
    22441 tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    22501 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    22561 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    22621 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    22681 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    22741 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    22801 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    22861 tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa gtgcctgcgg tgaaagccag
    22921 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    22981 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat
    23041 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    23101 aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga
    23161 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    23221 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    23281 tacttaagtc tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    23341 ctggttacca atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt
    23401 agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt
    23461 aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    23521 acggttaccg ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct
    23581 ttctattcag acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    23641 ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    23701 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    23761 tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    23821 aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    23881 cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    23941 gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    24001 cacccgtttg ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc
    24061 cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg
    24121 cgctatcatc gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa
    24181 agaattagac attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt
    24241 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
    24301 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
    24361 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
    24421 gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
    24481 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
    24541 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
    24601 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
    24661 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
    24721 atcatctgga taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca
    24781 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
    24841 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
    24901 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
    24961 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
    25021 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
    25081 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
    25141 gtatccacat catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta
    25201 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
    25261 atcttcaggg tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
    25321 atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
    25381 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
    25441 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
    25501 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
    25561 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
    25621 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
    25681 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
    25741 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
    25801 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
    25861 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
    25921 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
    25981 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
    26041 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
    26101 ggaacttaca acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac
    26161 acctgtaaag cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc
    26221 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
    26281 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
    26341 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
    26401 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
    26461 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc
    26521 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
    26581 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
    26641 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
    26701 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
    26761 agcattaaag acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
    26821 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
    26881 tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
    26941 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
    27001 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
    27061 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
    27121 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
    27181 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca
    27241 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
    27301 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
    27361 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
    27421 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
    27481 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
    27541 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca
    27601 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
    27661 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
    27721 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
    27781 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
    27841 gaggcacacc agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt
    27901 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
    27961 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
    28021 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca
    28081 ctggcggatg tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact
    28141 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
    28201 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
    28261 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
    28321 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
    28381 tactggctga ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg
    28441 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt
    28501 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
    28561 ctgaatgcag gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc
    28621 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
    28681 cgcccgttca cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta
    28741 agcagcctga ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
    28801 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
    28861 tacctgatct tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat
    28921 tcgctggctt ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
    28981 agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa
    29041 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
    29101 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
    29161 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
    29221 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
    29281 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
    29341 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
    29401 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
    29461 ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
    29521 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
    29581 tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa
    29641 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
    29701 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
    29761 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
    29821 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg
    29881 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg
    29941 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
    30001 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
    30061 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
    30121 gaaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
    30181 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
    30241 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
    30301 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
    30361 tcttcaatca tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc
    30421 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
    30481 ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
    30541 tattgctcgt ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc
    30601 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
    30661 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
    30721 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
    30781 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
    30841 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
    30901 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct
    30961 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
    31021 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
    31081 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
    31141 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
    31201 cagcattatg catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa
    31261 gcatatctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg
    31321 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
    31381 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
    31441 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
    31501 tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa
    31561 ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca
    31621 acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa
    31681 atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc
    31741 gtcaagtgat aatgcttatc aaaattatta tcacttgcac aaacactatc acgggatta
//
LOCUS       DAJTRQ010000033        31743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000033 DAJTRQ010000000
VERSION     DAJTRQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..685
                     /locus_tag="OO332_001624"
     CDS             <1..685
                     /locus_tag="OO332_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5942140.1"
                     /translation="YYIYDTKFLTLYLEQTEMKNLPIGGVWKGKVKLHSNSPAQDYFA
                     NITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNG
                     NAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVR
                     NNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVI
                     FTPSLPTIN"
     gene            complement(773..2071)
                     /gene="exuT"
                     /locus_tag="OO332_001625"
     CDS             complement(773..2071)
                     /gene="exuT"
                     /locus_tag="OO332_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5942141.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2554..3966
                     /gene="uxaC"
                     /locus_tag="OO332_001626"
     CDS             2554..3966
                     /gene="uxaC"
                     /locus_tag="OO332_001626"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5942142.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3981..5468
                     /gene="uxaA"
                     /locus_tag="OO332_001627"
     CDS             3981..5468
                     /gene="uxaA"
                     /locus_tag="OO332_001627"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5942143.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5551..6102
                     /locus_tag="OO332_001628"
     CDS             5551..6102
                     /locus_tag="OO332_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5942144.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6107..7351)
                     /gene="sstT"
                     /locus_tag="OO332_001629"
     CDS             complement(6107..7351)
                     /gene="sstT"
                     /locus_tag="OO332_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5942145.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7947..8912)
                     /gene="alx"
                     /locus_tag="OO332_001630"
     CDS             complement(7947..8912)
                     /gene="alx"
                     /locus_tag="OO332_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5942146.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9183..9383)
                     /locus_tag="OO332_001631"
     CDS             complement(9183..9383)
                     /locus_tag="OO332_001631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5942147.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9414..10181)
                     /locus_tag="OO332_001632"
     CDS             complement(9414..10181)
                     /locus_tag="OO332_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5942148.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10260..10952)
                     /locus_tag="OO332_001633"
     CDS             complement(10260..10952)
                     /locus_tag="OO332_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5942149.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11029..11532)
                     /locus_tag="OO332_001634"
     CDS             complement(11029..11532)
                     /locus_tag="OO332_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5942150.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11617..12753
                     /gene="rlmG"
                     /locus_tag="OO332_001635"
     CDS             11617..12753
                     /gene="rlmG"
                     /locus_tag="OO332_001635"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5942151.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13038..13352
                     /gene="higB"
                     /locus_tag="OO332_001636"
     CDS             13038..13352
                     /gene="higB"
                     /locus_tag="OO332_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5942152.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13349..13765
                     /gene="higA"
                     /locus_tag="OO332_001637"
     CDS             13349..13765
                     /gene="higA"
                     /locus_tag="OO332_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5942153.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13810..15828)
                     /gene="fadH"
                     /locus_tag="OO332_001638"
     CDS             complement(13810..15828)
                     /gene="fadH"
                     /locus_tag="OO332_001638"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5942154.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16048..18399)
                     /gene="ygjK"
                     /locus_tag="OO332_001639"
                     /pseudo
     CDS             complement(16048..18399)
                     /gene="ygjK"
                     /locus_tag="OO332_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18416..18835)
                     /locus_tag="OO332_001640"
     CDS             complement(18416..18835)
                     /locus_tag="OO332_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942155.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(18813..19166)
                     /locus_tag="OO332_001641"
     CDS             complement(18813..19166)
                     /locus_tag="OO332_001641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942156.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(19167..19487)
                     /locus_tag="OO332_001642"
     CDS             complement(19167..19487)
                     /locus_tag="OO332_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942157.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(19621..21054)
                     /locus_tag="OO332_001643"
     CDS             complement(19621..21054)
                     /locus_tag="OO332_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5942158.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21117..21566)
                     /locus_tag="OO332_001644"
     CDS             complement(21117..21566)
                     /locus_tag="OO332_001644"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5942159.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21563..24649)
                     /gene="ebgA"
                     /locus_tag="OO332_001645"
     CDS             complement(21563..24649)
                     /gene="ebgA"
                     /locus_tag="OO332_001645"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5942160.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24832..25815)
                     /gene="ebgR"
                     /locus_tag="OO332_001646"
     CDS             complement(24832..25815)
                     /gene="ebgR"
                     /locus_tag="OO332_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5942161.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26034..26365
                     /locus_tag="OO332_001647"
                     /pseudo
     CDS             26034..26365
                     /locus_tag="OO332_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26407..27786)
                     /gene="ygjG"
                     /locus_tag="OO332_001648"
     CDS             complement(26407..27786)
                     /gene="ygjG"
                     /locus_tag="OO332_001648"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5942162.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            28203..29723
                     /gene="aer"
                     /locus_tag="OO332_001649"
     CDS             28203..29723
                     /gene="aer"
                     /locus_tag="OO332_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5942163.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29877..30500)
                     /gene="nfeR"
                     /locus_tag="OO332_001650"
                     /gene_synonym="yqjI"
     CDS             complement(29877..30500)
                     /gene="nfeR"
                     /locus_tag="OO332_001650"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5942164.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30777..31523
                     /gene="nfeF"
                     /locus_tag="OO332_001651"
                     /pseudo
     CDS             30777..31523
                     /gene="nfeF"
                     /locus_tag="OO332_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31577..31652)
                     /locus_tag="OO332_001652"
     tRNA            complement(31577..31652)
                     /locus_tag="OO332_001652"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31617..31619),aa:Ile,seq:cat)
BASE COUNT         7678 a         8304 c         8331 g         7430 t
ORIGIN      
        1 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
       61 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
      121 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
      181 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
      241 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
      301 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
      361 gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga
      421 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
      481 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
      541 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
      601 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
      661 gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa
      721 acgacgaagc cgcctgataa ggcggcttcg tcgttataac ttacgttaaa cgttaatgtt
      781 gcggtgcggg atcgttatgt gtttcctgtg ccacctcaat cgccggtttg ttctgcaata
      841 cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg aacagcgggc
      901 tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc
      961 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     1021 aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca
     1081 gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca
     1141 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     1201 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     1261 tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg
     1321 ggatccacgc gttaaaagta ccccaggccg gttctgctag gaaacgcggc agtgcgatac
     1381 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct
     1441 ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct
     1501 ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac
     1561 cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc accagcggcg
     1621 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg atggaacgct
     1681 ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg
     1741 cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc agcgcggttg
     1801 caccacagaa cacggcccac agcacagcaa acattgcata accgattttc gtacccagca
     1861 catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa taggctgcga
     1921 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     1981 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca
     2041 tataccaacg taacccttta attttactca tgtaaaacct catcgttttg ttatatccgc
     2101 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     2161 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     2221 ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     2281 tgatttgcga cggcagaaag ataacttgtc atacaacctt aaaaggtgag agccatcaca
     2341 aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc
     2401 tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca
     2461 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     2521 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc
     2581 ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     2641 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     2701 ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg taccaacggt
     2761 gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     2821 gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcactt cgaactgcgt
     2881 cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     2941 gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg
     3001 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     3061 atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc
     3121 ttcaatatcg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     3181 accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc
     3241 gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg
     3301 aacgaagcgg aactcgacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag
     3361 cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt
     3421 cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc
     3481 aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat ggcggaagag
     3541 ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctctac
     3601 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     3661 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt
     3721 atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgttt tgtcggtatg
     3781 ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     3841 tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg
     3901 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     3961 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     4021 tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc
     4081 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca
     4141 acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     4201 acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     4261 ctgattttca ggatctgcct gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg
     4321 ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca
     4381 acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta
     4441 ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca
     4501 tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg
     4561 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata
     4621 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg
     4681 ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggaa
     4741 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     4801 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     4861 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg
     4921 acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag tactttattg
     4981 cccatgatca gccgatctac gaaaacccat caccggggaa caaagcgggc ggtatcacca
     5041 cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg
     5101 tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta
     5161 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     5221 ctggtcgcgg cacgccatac ggtggatttg tgccgacggt gaaaatcgcc accaacagtg
     5281 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta
     5341 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta
     5401 agcaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     5461 cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt
     5521 ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc ccagggcgtg
     5581 ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc
     5641 aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc
     5701 tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg
     5761 cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag
     5821 ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg
     5881 ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg
     5941 attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag ttttctgctt
     6001 atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt
     6061 aaagtagaga aaacccctcc cgccatagac gaaaggggtt aaacaattaa ttacgcaggg
     6121 cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag
     6181 ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgataata aagccgacgg
     6241 caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc agtgggatca
     6301 gcagcaaaga accccccgcc acgccggatg cgccacaggc acacagagaa gccactacgc
     6361 tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc gcagccagcg
     6421 tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggctccc agcggaatag
     6481 aaactgaata ggtatcgcga tccagattca gcttttcaca cagcgccata ttcaccggaa
     6541 tgttcgctgc agagctgcgg gtgaagaagg catacacacc gctttcgcgc aggcacagca
     6601 gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca
     6661 gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg
     6721 tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg atcggtgcga
     6781 agcgaatgac cagtttcacc ataaaggtaa cggcattcga catatcattg accaggtttt
     6841 tggtggtctc gttaccgtga cgcagtgcga agccgaggcc gatcgcccac accagaatcc
     6901 cgatgtagtt acctttcagc agggcgtcga tggggttgga aaccatgctc attaccagtc
     6961 cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta ctggacaagt
     7021 gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagctgca gagaaggtgc
     7081 ccagcagata gaggaacaaa ataggacgga tattggtttt ctgcccgtgc tggtggttag
     7141 caatagatgc cattaccagc atcaacacca ggatgggggc aacagctttc agtgcgccga
     7201 cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa
     7261 gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc
     7321 ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac
     7381 atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga
     7441 gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcatg
     7501 ttttgcaact taatggttgc atttatttaa cataaacaga gggaaggact ggctattaag
     7561 gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg cgctccttta
     7621 acgtgttatg ccatataaag tcactggcgc agggagcgcg cagggggcgg ccaatcgccg
     7681 ccgccccctg cccccccagg ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc
     7741 tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc
     7801 ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc ggtctttttg
     7861 taaagagcta taagccgatt acataattaa tagctcacct ttgtctggaa tgagttatct
     7921 ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg ccgataattc
     7981 acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc cagcgagacg
     8041 gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa caccagaatc
     8101 accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag caggaaatac
     8161 atcgcacgca ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg
     8221 gtcacggcga agatagccgg aatgctatcc acggcaaaaa tcacgtcgct caattccacc
     8281 agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg cacaaagaaa
     8341 tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc
     8401 ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc
     8461 aggaaggcac cgaagatata caggatccag tcgaactgcg aaatcagcca gctgcctgtg
     8521 aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag cacgcgacgt
     8581 tgtaatgccg ccggaacgga gaaatagctg aacaacatca gccagacaaa gacgttatcg
     8641 accgccagcg atttctcaat cagataaccc gtgagaaagg ccagggcctg tggatcggca
     8701 acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa cagtaacgac
     8761 agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc atgtgcccca
     8821 cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac agcaaatccg
     8881 ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata aaaaaacggc
     8941 caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc cggcaaggtc
     9001 tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc ggtgaccgga
     9061 tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc ccctttgcag
     9121 gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc ttttacactg
     9181 ttttactttg gcaagtatag ttttacgcta tctgccggaa aaatcacccc agtctggcgg
     9241 cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg atgatccacc
     9301 agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt attaatatgc
     9361 tgcggctggg tgagatcctg catctggctg ccacgcggca caaagcagac tttctagcat
     9421 tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct tcccaggaca
     9481 gaatcactga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc catcaccacc
     9541 acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac gcttttcggc
     9601 tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat aattgaaccg
     9661 ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc
     9721 gaggaatact ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc
     9781 tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa cagcaccacc
     9841 tgattttcgc gcgcacaggc aatggcggca tccacttccg ccagattcga cgccagcggt
     9901 ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga aaagtgcagg
     9961 gaattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat cgcttccagc
    10021 gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg
    10081 cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc gacgaactgg
    10141 cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg atactccata
    10201 aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc agatacgcat
    10261 tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga tggcccgaga
    10321 tatttcggct ggcgatggag gatttttgcg tcaatcaccg cgctgacacg agcgagtttc
    10381 tcccgcagtt gagtataaaa accatgcttc atattgaggt ctggcgcgtt aaacccgata
    10441 gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa ttcttgcgag
    10501 ataatggtga tatggttttc cccaaacacc tttttggcac gcactaccga atccagcgtt
    10561 gagaatccgg catagtcgca aaagatcact ttcgccggta cgcctttagc gatcaaagcc
    10621 tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt tatcgccact caccagtagc
    10681 catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg ggtaaagtag
    10741 cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccgggac ggcgttgaca
    10801 tcgccccagg tcagctgctt gctggcgttg accataacgc gatcggcgat aaaaatggtc
    10861 gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga aaaacaaata
    10921 cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga aaggggggag
    10981 gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc
    11041 ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct ggtggtactg
    11101 cggctccata tggcagcaca attgatagaa cgctttgtta tgctctttct ccttcaggtg
    11161 cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt
    11221 agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcatacgag aaacagcggt
    11281 atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca ctttattgat
    11341 cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc agagagcttt
    11401 atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg
    11461 ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg ggataaccct gaagataagt
    11521 aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca
    11581 ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc
    11641 cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg
    11701 gaagcggcag atgaatattt gctgcaacag ttggacgaca cagaaatccc cggcccggtg
    11761 ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac
    11821 agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg cctaaacggg
    11881 attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca acagccgggt
    11941 gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact gcgtgcgctg
    12001 cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg tgacattcac
    12061 acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg
    12121 aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag
    12181 accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc gaatgtcttc
    12241 tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc agagaatctc
    12301 gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac gctgcttgat
    12361 aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc
    12421 cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt tatgatcaac
    12481 aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa ccctccgttt
    12541 caccaacagc atgcgctgac cgataacgtc gcctgggaga tgttccacca cgcccgccgc
    12601 tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gccatctgga ttacttccat
    12661 aaactgaaga aaattttcgg caactgcacc actattgcga cgaataataa atttgtggtg
    12721 ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg agttacctgc cggatgcggc
    12781 gtaaacgcct tatccggcct acgttcagca cgttaccccc aggccggata agacgcggca
    12841 agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat
    12901 ccctgtcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat tcttccgtca
    12961 cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg ccaatttgtc
    13021 aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat gctgcggaaa
    13081 aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct aagggatatt
    13141 tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc aaatatctgg
    13201 ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca atggtctttt
    13261 ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac gatttcttta
    13321 ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg
    13381 agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg aacaatacac
    13441 ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga
    13501 tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg cggaatttaa
    13561 tgccatggct caagccatgc ctggcggtat cgccgtgatt cgtaccctta tggatcaata
    13621 tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt
    13681 gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat
    13741 ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc cggcgcttca
    13801 aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc
    13861 cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag
    13921 cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc
    13981 taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata
    14041 acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc
    14101 ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatttg
    14161 ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgtaggct
    14221 gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg attcgcccgg
    14281 ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga tggcaacctt
    14341 gttgcctacc ggcgctttgt cgcgcagtac atcgagataa ctcaatacct tcggatgatc
    14401 gatcccgtcg ataggcggaa cacgcggcac gatcccactg gcgaggatcg tttcatcgaa
    14461 cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc
    14521 gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt
    14581 ggcgatatta aactgcccgc cgatctcgct gtgcgcatca aacaatgtta cctgatgccc
    14641 acgcgccgcc gcgttaatgg caaacgccag cccagcaggt ccagcgccga ccaccgccag
    14701 attttttttc tgcacggcgg gaaggattgg cattttggtt tcgtggcagg cgcgaggatt
    14761 caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct ggttgcagcc
    14821 aatacaagtg ttgatctcat cggctcgccc tgattgcgct tttgacagca gttccgcatc
    14881 cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc
    14941 ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga cgtggccttt
    15001 cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat
    15061 ccgcgcttcg tgccagccta ttccggtgtt gataatggtc gcgcccgccg cttcaatggc
    15121 ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt cgagcatcga
    15181 caaccggtaa ataataatga agtcgttgcc gacgcgttcg cgcaccgtac gcactacttc
    15241 tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac gctgattggt
    15301 gcgcagcgtc agaaattcgt tgatcaaata cccttcagag cccatcactt cgacgccatc
    15361 gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat
    15421 ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca atgcggacgg
    15481 tgcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggcgatttt
    15541 gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc
    15601 gttgagcatt gcgccacctt ccatgccaac gcctattaaa tctggtgcaa tgccgccgct
    15661 gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgcagcca gccgttcagc
    15721 accgtccggg tattcctcca gcccggtgtg cattgaaccc atcaacacgc ggttttttaa
    15781 cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag
    15841 tatgtaaaat aattgttacg gatgagttct aatttagccg tcgatggaga aaagggaaaa
    15901 ggggagtgcg gggtttgtga tgggattcaa aaagaatacg cccggtggta acgccggatg
    15961 cgacgcttgc cgtgttttat ccggcttggg ggattgcaca tgtagggcgg ataaggcgtt
    16021 cacgccgcat ccgccagccg ttgcaaatta ctgcttacgg aaaaaatcgt tatacaacat
    16081 atacaaatga gcggcactcc aggagaaatt tggtgcgcct tgctgcgcgc ctgtcagcgg
    16141 gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgcc tgaagaacgt
    16201 atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt tcagaccaaa
    16261 ccagaactga tccacccata cgcgcccgcg ccagtagata tcagcgccaa aagccggatt
    16321 ggttaacgcc gccgttccca gcggaacaaa ggtgttgaac tctttaggat cgagcatcac
    16381 cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg gcgaccagcc
    16441 ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg ccagtggttt
    16501 atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac aggtgttgat
    16561 gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg gcttaccgag
    16621 gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg cctgatccac
    16681 cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt tttcggcgaa
    16741 tttcaccgtc tagtcgctac gtttgccgcc attagcgaca tatttatcca actgttcttt
    16801 gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg cagcgacctg
    16861 tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt agttgttcag
    16921 cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct cgccgctctc
    16981 ggtgttgtgg gctttgtcgc gggtcgcgcc atattccggc accccgttgc cgttatgatc
    17041 gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct ctgccaacca
    17101 ggctttatcc tgggtgacgt tgtatacttc catcaccgac caggcggcaa ggctgggttt
    17161 ggtattacgt tcgttccagt tgccgccatc gccaccacgc tcggggctaa ggttccacgc
    17221 tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccag gctggatctg
    17281 ccaggagaag accgcgcgga tattctcttt ggcgatatcc ggattgaaat gcgccatcgc
    17341 aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc agcgtccggt
    17401 caccgacggc gtgacggtgt tataattcac cgcaccgccc ggtgagcgcc agttaccgtt
    17461 gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat ccggattggt
    17521 taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat aaaacgccgg
    17581 acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag cggtcagcag
    17641 atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac tggtaaagcg
    17701 attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt ccgattcgcc
    17761 ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca ggccatcacg
    17821 ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt tatcggaaag
    17881 cggtttccct tctttcgctt ccagtttttc cagcagttcg ccatcccaca ccagatccag
    17941 cggtttattg ctgatgattt tagtttccag tagtgacgtg cgcggcgtgg cgaagcgcag
    18001 agtcatttcg acctgcacat cttttgctgt cagtttttgc accagcgcgc cgggaatact
    18061 gtatgcctcc agcgtgaagt tgactttctt gccgtcttgc cagacagtca ggcggtcgaa
    18121 attgctggcc ataaagttga tgtattcttc cgtcagcagc gcaacgcccg gaaagccgcc
    18181 catggtgttc gggccgtctg gcaacagatg accatgccag gctccgagat cgaaaaacgg
    18241 attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg caccagtacg
    18301 gttaatcacg tttttataat tgtcggcgtt agcggcatga gcggaaaaac ttatcagcag
    18361 agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt ccttattacc
    18421 agtagaaata ttgcatcagg aagatttggt cgctgggatc gttagaggca tttttcatgt
    18481 agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc atattcgtac cagataccaa
    18541 actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg gcgtaggtgt
    18601 aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc atcatcccgc
    18661 tttcacttcc cggtacgtcc agcccgtcgg cacgtccggt atggtgccag gcgcgagcgg
    18721 caaagttagg ggtaaacagg cccgtgagat agagttgtcc tgtgccgtcg gtaatttcca
    18781 gaccattcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg ttcatccatt
    18841 gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag ccaggctttg
    18901 ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt gccgcttaag
    18961 ttctgtacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta gcccaaatcg
    19021 ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg cagactgagc
    19081 atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat cccaccttcg
    19141 aaaccacccc agctatggtc aggcacctag gcattaccgt cgtggtcggc ctgcacggtc
    19201 cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag gccaccttca
    19261 atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat atatccacca
    19321 agccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat tgcgggtgtt
    19381 tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa ggcgtagcaa
    19441 aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt gttaatccat
    19501 gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa aggcagaaac
    19561 tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt attagtctgc
    19621 ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg ttaatccctt
    19681 tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac cgccgacgtt
    19741 ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga caaaccctac
    19801 ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg agcccatacg
    19861 gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca gcatgatgaa
    19921 taacggcgga agcatggacg ctgcggcggt catattaata atggtattca tcagatcctg
    19981 cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca gaaactggat
    20041 ccatgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct taccaaagat
    20101 gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca tcagcaggga
    20161 accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg cttccggcag
    20221 accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccggtaa atttcaactc
    20281 tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatacagta cgccgataaa
    20341 aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga cgtcgttaac
    20401 gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg taacgccgag
    20461 gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg caggctgtac
    20521 gtcgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca gcagcatcag
    20581 cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg aaacccaggt
    20641 ggagaaggcg aacagcacca tactgataac ggtggtggca accggcgtca taatatattc
    20701 gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg aggtgaaaaa
    20761 gaacagattt acgaaccagt aagtgtaggc agtaataaat gcccaacgtc cgcccagcga
    20821 actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa caaattctgc
    20881 gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca tcggtgccga
    20941 ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa cggcggcaaa
    21001 agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag acataacaca
    21061 tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct tttgccgccg tagttgttat
    21121 ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt gaccgcgtta
    21181 ttgcagataa accgatacgc ttcatggata tcgcaaataa cgatttgccc ttcgtggacc
    21241 tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg ataatattcc
    21301 actacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg caggtagtaa
    21361 tgcacttcaa aatagcgtcg atgcccggta aatagcgcat gggtcgcgga gtcattttcc
    21421 acacggtagg tcaatgaatc accgatggag tgggcgacgc caggctgaat gttgtcgata
    21481 ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat ctggcggaac
    21541 tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc gtggaaaaga
    21601 accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg ccagaaaccg
    21661 gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag tccagcacct
    21721 cgctgcccca ggagttggag ccgaggccaa gtagctggtg gtcgaggttc agggtgatgt
    21781 catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc tgggtatagt
    21841 gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg tcgtggcggt
    21901 tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg aagggatagt
    21961 tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc tgctggctgt
    22021 cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat tcgccgttaa
    22081 tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa cgctcgccgg
    22141 aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggta cagcgcatcc
    22201 cgaagtcaaa caccggcggt gcaataactg tgcggctgat gatcaacact tcaccatcgc
    22261 tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga ttcggctgcc
    22321 acaacccttc gtactcctgc ttgtggttgt cgatcatcgg cttgaagaag ttgatctttg
    22381 gctcgcgggt cagcagcgac tcgccattta cctgccagga tgtcggtttg ccactcattt
    22441 ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca gctcaaacga tcgtcttcca
    22501 gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg ctgcgcggtg ttttccttca
    22561 gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg cgggaatctt
    22621 tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc gtgatctgca
    22681 agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgctgcgtc gcgagcgttt
    22741 caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta aaccacagtt
    22801 tattttcgac tttcaactcg ccgcgagtca gcgcgtggat ttttaccggc gcgataacct
    22861 gtttgtactc tttcaggccc gatcccggcg tctgatcgga atagatcaaa ccatcaagac
    22921 agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac cagacattgc
    22981 cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa tgaccctgaa
    23041 tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt ccgttcccca
    23101 tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca ccaaactcat
    23161 tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca gcatcgcgat
    23221 cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg tacatcgcgc
    23281 ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc gacggatggt
    23341 ttttctgtgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc cactgcggat
    23401 cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg acgtcggttt
    23461 ccgccatcac aaacaggccg tagatatcac acaattcgta aaaacgcgga tcgttcgggt
    23521 agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga tctttctcga
    23581 cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg ttgacgccgt
    23641 gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg atatcacgga
    23701 agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc agcgtcatga
    23761 ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg acagtaaaag
    23821 caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg ctgtgcacca
    23881 cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag gcggcgagat
    23941 tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca aagtcggtac
    24001 gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca tcgcggaaga
    24061 tccccgccga ccaccacata tcctggtctt ccacgtaggt agagtccgcc cactgcatca
    24121 cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca aactctgcgg
    24181 tcaggcgact gcccttgctg aaacccacat actgaccgtt aacgtagact tcaaaatagg
    24241 tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg ctgagggtga
    24301 aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca tcgatgggga
    24361 acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc cacatggcgg
    24421 ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct ggtacttgca
    24481 gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac aggctgctgg
    24541 tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag tacgcacgcg
    24601 gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata ggacctcccg
    24661 gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct gggggggtta
    24721 gtaaaatttt tactaaaagt agcaggtaaa tgcatttact ttaactggat cacgaaaata
    24781 aacggcagca ctgtccggca aaaatgattg ccggacagtg tgtaagggga tttaacgggt
    24841 cgtgccgcgc agttttaatt tgctgggaac gaagactagc agcggcagcg cgcgaccatc
    24901 gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg aatggatgcg
    24961 cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa cgctgataag
    25021 cgaaatatct tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta cgccgatagc
    25081 aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca gcatttgttt
    25141 tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct cttcgcgtac
    25201 cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg ccttgccagg
    25261 ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat agaagtcgat
    25321 gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc tgccgggttc
    25381 gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc gcagggcggg
    25441 cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta atccgttgtg
    25501 ttcataacag ttggtcagtt cgatcgccag cttttcgcac tgagtttcaa tgccgtggcg
    25561 gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt agatagccag
    25621 aatatggtgt tggttgactg cgcctgtctg tagtttacgg gcgctactgg tcttgtactc
    25681 cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg
    25741 atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga ttgcgatgtc
    25801 ttttaatgtt gccatgttta ccttttttac tttccctgat gtgcgccccc attgtagagg
    25861 aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga cgctgtaacg
    25921 gtaacacagc ggcttaggaa tgtgatcacg cctgcattct ccatttttag cctacaggat
    25981 tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa gcaatggaaa
    26041 ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga
    26101 aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg aaaaaacact
    26161 gcaaaccgtg accagcttgg tgccgtacta cagcgaagag gagctgatgg ggaaaacggt
    26221 ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg aatgcatgct
    26281 gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg agcggatgat
    26341 gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc gccatccgat
    26401 ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac gcgccgcttt
    26461 gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc ggatcgtttt
    26521 ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc tggcaaagtt
    26581 atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac cacgcgcttc
    26641 ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca acatatcgcc
    26701 tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg tcgccagcgc
    26761 cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg ggttgtcgaa
    26821 cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca cgccgccgcc
    26881 gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc aggcaaacat
    26941 cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg cgccgaactc
    27001 atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta cgccaccttc
    27061 gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt tgcactcatt
    27121 aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc ctggcagcag
    27181 tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc caagtgattt
    27241 accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg actgataagc
    27301 cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga agaagctgta
    27361 tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc gtagcggatc
    27421 gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg cggaaaccac
    27481 aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt cgataaactc
    27541 ctgtccttgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta cggctccgta
    27601 atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga catgctcttt
    27661 gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca gcgttcgctt
    27721 ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg aaggtaacct
    27781 gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt attgcaggga
    27841 ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg ttatcatttg
    27901 tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt tgtcgggtat
    27961 ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct gatttttggg
    28021 caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc aaagaattag
    28081 atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa gataaccgca
    28141 gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca caggacgcga
    28201 acatgtcttc tcatccgtat gtcacccagc aaaatacgcc gctggcggac gataccactc
    28261 tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact tttgtgcagg
    28321 tgagcggctt taccttgcaa gagttacaag ggcagccgca caacatggtg cgtcacccgg
    28381 atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg gagccctgga
    28441 gcggcatcgt gaaaaatcgc cgcaaaaatg gcgaccatta ttgggtgcgg gccaatgcgg
    28501 taccgatggt gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc cgggcgacgg
    28561 atgaagagat tgcggcggtg gagccgctgt acaaagcgtt gaactccgga cgtaccagta
    28621 agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactg ccttcattac
    28681 cgcttcgctg gcggacgcgt ggagtgatga ccctgatgtt tatcttgctg gcggccatgc
    28741 tttggtttgt tgctgccccg gtggtgacgt atttcctctg tgtgttagtg gtattgttgg
    28801 caagcgcttg ttttgaatgg cagattgtgc gcccgataga aaatgtcgcc cgtcaggcac
    28861 tgaaggtggc gaccggagag cgtaatagtg ttgagcacct gaatcgcagc gatgagctgg
    28921 ggctgacatt acgcgcggta gggcagcttg gcctgatgtg ccgttggcta attaacgatg
    28981 tctcaagcca ggtgtccagt gtcagaaatg gcagtgagac gctggcgaaa ggcaccgatg
    29041 aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg gcgaccatga
    29101 accaaatggc ggcgtcggtg aaacagaact ctgccacggc gtcggctgcc gataaacttt
    29161 ctatcaccgc cagtaatgcg gcagtgcagg gtggggaggc gatgaccacg gtgatcaaga
    29221 caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg ctgattaacg
    29281 atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg gcgcgtgccg
    29341 gcgaacaggg caaaggtttt gcagtggtgg caggggaagt gcgtcattta gccagccgca
    29401 gcgctaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat aaggtgcaat
    29461 ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg gcacaggtga
    29521 aaaacgtcac ccagttgatc gcccagatta gccattcaac gctggaacag gccgatgggc
    29581 tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa aatgccgagc
    29641 tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc cgactggaag
    29701 acgcggtgac ggtgctgcat taatcggtat gccggatccg gcggttggag cacaatgcct
    29761 gatgcgatgc tgacgcatct tatcaggcct gcggggtgtg tagcgggtgt aggccggata
    29821 aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg cggcgattaa
    29881 tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt ttttatttgt
    29941 gcatcactga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa attatccagc
    30001 gcccgcttca tttgcgggtt ctggcgcagc gccgcgccaa cgcaacgcgc tttgatgcgt
    30061 tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc ttgttcggtc
    30121 agcgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga ctgctcctgc
    30181 agaaaatcca gcgtcgggta gatgacgccc gggcttgggg tgtaattccc ctgggttaga
    30241 ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag aatatccaga
    30301 atcaccagac gcaattcacc gtgaccaaag aaacgttgcc gacgaccgcc gccgcgaccg
    30361 tggcgaccac cgcaacattg accgtgttca tgctggtgac cgtgtccgca gtgctcgtgt
    30421 tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg tttacaacac
    30481 ccttcgtgat gatggctcat atcagcctcc ttttgcttga ttagatatat ctaatttata
    30541 tctgatgtga gcattactgc aagtacaaaa atgattttta aacgtggaag cacctcacat
    30601 ctaggccgga taagacgttc acgccgcatc cggcagcatt gctatggcac tgaatgcctt
    30661 cacatacttc agacatatca gttctacaaa tcgcttgcat ttatcatgat taacaatcat
    30721 tatcatttgc gaattttatt tagatatatc tgattaccat cacgaaggcg ataacaatga
    30781 ataacacccc ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt gaactgactg
    30841 tgctacgcgt tgagcgcatc agcgccggtt ttcagcgcat tgtccttggc ggcgaggcgt
    30901 tggacggttt tacatcgcgc ggctttgacg atcacagcaa actcttcttt cctcaacctg
    30961 acgctcactt tgtgccgcca acggtaacgg aagagggcat cgtctggccg gaaggaccac
    31021 gcccaccgtc gcgtgactat acgccactgt atgacgaact acgccatgaa ctggcgatcg
    31081 atttctttat tcacgacggt ggggtcgcca gcggctgggc gataaactta cggtggcagg
    31141 tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg tctgcgatga
    31201 atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc
    31261 gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc tcgcgcacct
    31321 tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg cgcgtctggc
    31381 gcagatgcaa atccctgcca gatgattact tcatctggat aaccggcgaa ggtaaagtcg
    31441 ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg gtacgtgcag
    31501 cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac gatttctcgt
    31561 cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa gcgattatga
    31621 gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa gtaactccgt
    31681 gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat tttcaatccg
    31741 tta
//
LOCUS       DAJTRQ010000034        30090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000034 DAJTRQ010000000
VERSION     DAJTRQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..96
                     /locus_tag="OO332_001653"
     CDS             <1..96
                     /locus_tag="OO332_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942165.1"
                     /translation="IHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            326..709
                     /gene="secE"
                     /locus_tag="OO332_001654"
     CDS             326..709
                     /gene="secE"
                     /locus_tag="OO332_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5942166.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            711..1256
                     /gene="nusG"
                     /locus_tag="OO332_001655"
     CDS             711..1256
                     /gene="nusG"
                     /locus_tag="OO332_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5942167.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1415..1843
                     /gene="rplK"
                     /locus_tag="OO332_001656"
     CDS             1415..1843
                     /gene="rplK"
                     /locus_tag="OO332_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5942168.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1847..2551
                     /gene="rplA"
                     /locus_tag="OO332_001657"
     CDS             1847..2551
                     /gene="rplA"
                     /locus_tag="OO332_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5942169.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2964..3461
                     /gene="rplJ"
                     /locus_tag="OO332_001658"
     CDS             2964..3461
                     /gene="rplJ"
                     /locus_tag="OO332_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5942170.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3528..3893
                     /gene="rplL"
                     /locus_tag="OO332_001659"
     CDS             3528..3893
                     /gene="rplL"
                     /locus_tag="OO332_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5942171.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4213..8241
                     /gene="rpoB"
                     /locus_tag="OO332_001660"
     CDS             4213..8241
                     /gene="rpoB"
                     /locus_tag="OO332_001660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5942172.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8318..12541
                     /gene="rpoC"
                     /locus_tag="OO332_001661"
     CDS             8318..12541
                     /gene="rpoC"
                     /locus_tag="OO332_001661"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5942173.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12783..13916)
                     /gene="thiH"
                     /locus_tag="OO332_001662"
     CDS             complement(12783..13916)
                     /gene="thiH"
                     /locus_tag="OO332_001662"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5942174.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13913..14683)
                     /gene="thiG"
                     /locus_tag="OO332_001663"
     CDS             complement(13913..14683)
                     /gene="thiG"
                     /locus_tag="OO332_001663"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5942175.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14685..14885)
                     /gene="thiS"
                     /locus_tag="OO332_001664"
     CDS             complement(14685..14885)
                     /gene="thiS"
                     /locus_tag="OO332_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5942176.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14869..15624)
                     /gene="thiF"
                     /locus_tag="OO332_001665"
     CDS             complement(14869..15624)
                     /gene="thiF"
                     /locus_tag="OO332_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5942177.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15617..16252)
                     /gene="thiE"
                     /locus_tag="OO332_001666"
     CDS             complement(15617..16252)
                     /gene="thiE"
                     /locus_tag="OO332_001666"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5942178.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16252..18147)
                     /gene="thiC"
                     /locus_tag="OO332_001667"
     CDS             complement(16252..18147)
                     /gene="thiC"
                     /locus_tag="OO332_001667"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5942179.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18228..18328)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18381..18857)
                     /gene="rsd"
                     /locus_tag="OO332_001668"
     CDS             complement(18381..18857)
                     /gene="rsd"
                     /locus_tag="OO332_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5942180.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18952..19725
                     /gene="nudC"
                     /locus_tag="OO332_001669"
     CDS             18952..19725
                     /gene="nudC"
                     /locus_tag="OO332_001669"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5942181.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19765..20829
                     /gene="hemE"
                     /locus_tag="OO332_001670"
     CDS             19765..20829
                     /gene="hemE"
                     /locus_tag="OO332_001670"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5942182.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20986..21657
                     /gene="nfi"
                     /locus_tag="OO332_001671"
     CDS             20986..21657
                     /gene="nfi"
                     /locus_tag="OO332_001671"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5942183.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21700..22290
                     /locus_tag="OO332_001672"
     CDS             21700..22290
                     /locus_tag="OO332_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5942184.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22477..22749
                     /gene="hupA"
                     /locus_tag="OO332_001673"
     CDS             22477..22749
                     /gene="hupA"
                     /locus_tag="OO332_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5942185.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22762..23457
                     /locus_tag="OO332_001674"
     CDS             22762..23457
                     /locus_tag="OO332_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5942186.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23459..23884)
                     /gene="zraP"
                     /locus_tag="OO332_001675"
     CDS             complement(23459..23884)
                     /gene="zraP"
                     /locus_tag="OO332_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5942187.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24122..25498
                     /gene="zraS"
                     /locus_tag="OO332_001676"
     CDS             24122..25498
                     /gene="zraS"
                     /locus_tag="OO332_001676"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5942188.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25495..26820
                     /gene="zraR"
                     /locus_tag="OO332_001677"
     CDS             25495..26820
                     /gene="zraR"
                     /locus_tag="OO332_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5942189.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26817..28106)
                     /gene="purD"
                     /locus_tag="OO332_001678"
     CDS             complement(26817..28106)
                     /gene="purD"
                     /locus_tag="OO332_001678"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5942190.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28118..29707)
                     /gene="purH"
                     /locus_tag="OO332_001679"
     CDS             complement(28118..29707)
                     /gene="purH"
                     /locus_tag="OO332_001679"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5942191.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7040 a         7845 c         8283 g         6922 t
ORIGIN      
        1 atccacccga tcgcgatgga cgacggtctg cgtttcgcaa tccgtgaagg cggccgtacc
       61 gttggcgcgg gcgttgtagc aaaagttctg agctaattgc cgataacatt tgacgcaatg
      121 cgcactaaaa gggcatcatt tgatgccctt tttgcacgct ttcgtaccag aacctggctc
      181 atcagtgatt ttctttgtca taatcattgc tgagacaggc tctgttgagg gcgtataatc
      241 cgaaaagcta atacgcgttt cgatttggtt tgcctcgcga tcgcggggtg aaaatgtttg
      301 tagaaaactt ctgacaggtt ggtttatgag tgcgaatacc gaagctcaag gaagcgggcg
      361 cggcctggaa gcgatgaagt gggtcgttgt ggtggcattg ctcctggcgg cgattgtcgg
      421 caactacctt tatcgcgaca ttatgctgcc gctgcgtgcg ctggccgtag taattctgat
      481 tgctgcagcg ggtggtgtcg cgctgttaac gacaaaaggt aaagctaccg ttgcttttgc
      541 ccgtgaagcg cgtaccgaag tccgtaaggt catttggccg actcgccagg aaacattgca
      601 caccacgctg attgtggctg cggttaccgc agtaatgtca ctgatcctgt ggggactgga
      661 tggtattctg gttcgcctgg tatcctttat cactggcctg aggttctgag atgtctgaag
      721 ctcctaaaaa gcgctggtac gtcgttcagg cgttttccgg ttttgaaggc cgcgtagcaa
      781 cgtcgctgcg tgagcatatc aaattacaca acatggaaga tttgtttggt gaagtcatgg
      841 taccaaccga agaagtggtt gaaatccgtg gcggtcagcg tcgcaaaagc gaacgtaaat
      901 tcttccctgg ctacgtcctc gttcagatgg tgatgaacga cgcgagctgg cacttggtgc
      961 gcagcgtacc gcgtgtgatg ggcttcatcg gcggtacttc cgatcgtcct gcgccaatca
     1021 gcgataaaga agtcgatgcg attatgaacc gcctgcagca ggttggtgat aagccgcgtc
     1081 cgaaaacgct gtttgaaccg ggtgaaatgg tccgtgttaa tgatggtccg ttcgctgact
     1141 tcaacggtgt tgttgaagaa gtggattacg agaaatctcg tctgaaagtg tctgtttcta
     1201 tcttcggtcg tgcgaccccg gtagagctgg acttcagcca ggttgaaaaa gcctaaccca
     1261 gcgatcaaaa aagcggcgat ttaatcgttg cacaaggcgt gagattggaa tacaatttcg
     1321 cgccttttgt ttttatgggc cttgcccgta aaacgatttt ttatatcacg gggagcctct
     1381 cagaggcgtt attacccaac ttgaggaatt tataatggct aagaaagtac aagcctatgt
     1441 caagctgcag gttgcagctg gtatggctaa cccgagtccg ccagtaggtc cggctctggg
     1501 tcagcagggc gtaaacatca tggaattctg caaagcgttc aacgcaaaaa ctgattccat
     1561 cgaaaaaggt ctgccgattc cggtagtaat caccgtttac gctgaccgtt ctttcacttt
     1621 cgttaccaag accccgccgg cagcagttct gctgaaaaaa gcggctggta tcaagtctgg
     1681 ttccggtaag ccgaacaaag acaaagtggg taaaatttcc cgcgctcagc tgcaggaaat
     1741 cgcgcagacc aaagctgccg acatgactgg tgccgacatt gaagcgatga ctcgctccat
     1801 cgaaggtact gcacgttcca tgggcctggt agtggaggac taagaaatgg ctaaactgac
     1861 caagcgcatg cgtgttatcc gcgagaaagt tgatgcaacc aaacagtacg acatcaacga
     1921 agctatcgca ctgctgaaag agctggcgac tgctaaattc gtagaaagcg tggacgtagc
     1981 tgttaacctc ggcatcgacg ctcgtaaatc tgaccagaac gtacgtggtg caactgtact
     2041 gccgcacggt actggccgtt ccgttcgcgt agccgtattt acccaaggtg caaacgctga
     2101 agctgctaaa gctgcaggcg cagaactggt aggtatggaa gatctggctg accagatcaa
     2161 gaaaggcgaa atgaactttg acgttgttat tgcttctccg gatgcaatgc gcgttgttgg
     2221 ccagctgggc caggttctgg gtccgcgcgg cctgatgcca aacccgaaag tgggtactgt
     2281 aacaccgaac gttgctgaag cggttaaaaa cgctaaagct ggccaggttc gttaccgtaa
     2341 cgacaaaaac ggcatcatcc acaccaccat cggtaaagtg gactttgacg ctgacaaact
     2401 gaaagaaaac ctggaagctc tgctggttgc gctgaaaaaa gcaaaaccga ctcaggcgaa
     2461 aggcgtgtac atcaagaaag ttagcatctc caccaccatg ggtgcaggtg ttgcagttga
     2521 ccaggctggc ctgagcgctt ctgtaaacta atgcctttac gtgggcggtg attttgtcta
     2581 caatcttacc cccacgtata atgcttaatg cagacgtata tccgagatat tcgggttgtg
     2641 gcaaggcggc aactgagtga gtcgccagga gcatagctaa ctatgtgact ggtgcgaatg
     2701 aaggaagcca acgccgccac aagttgaata gcgacggata gaaaagattt gttcgttgga
     2761 gcctggccta tccaggcctc cgtcgaagac cgcaggagtt tcgcaagaaa cttaatcccc
     2821 tgcgtagacg gtgacagaac gctaagatta ttcttttata ttctggcttg tttctgctca
     2881 ccgtaattaa gacgctctct ccgtttggag gagtgaagtg agttccagag attttctctg
     2941 gcaaacatcc aggagcaaag ctaatggctt taaatcttca agacaaacaa gcgattgttg
     3001 ctgaagtcag cgaagtagcc aaaggcgcgc tgtctgcagt agttgcggat tcccgtggcg
     3061 taactgtaga taaaatgact gaactgcgta aagcaggtcg cgaagctggc gtatacatgc
     3121 gtgttgttcg taacaccctg ctgcgccgtg ctgttgaagg tactccgttc gagtgcctga
     3181 aagacgcgtt tgttggtccg accctgattg catactctat ggaacacccg ggcgctgctg
     3241 ctcgtctgtt caaagagttc gcgaaagcga atgcaaaatt tgaggtcaaa gccgctgcct
     3301 ttgaaggtga gctgatcccg gcgtctcaga tcgaccgcct ggcaactctg ccgacctacg
     3361 aagaagcaat tgcacgcctg atggcaacca tgaaagaagc ttcggctggc aaactggttc
     3421 gtactctggc tgctgtacgc gatgcgaaag aagctgctta atcgcagtta tctttttaac
     3481 gcattcgctt acgtataaac ttattctgat attcaggaac aatttaaatg tctatcacta
     3541 aagatcaaat cattgaagca gttgcagcta tgtctgtaat ggacgttgta gaactgatct
     3601 ctgcaatgga agaaaaattc ggtgtttccg ctgctgctgc tgtagctgta gctgctggcc
     3661 cggttgaagc tgctgaagaa aaaactgaat tcgacgtaat tctgaaagct gctggcgcta
     3721 acaaagttgc tgttatcaaa gcagtacgtg gcgcaactgg cctgggtctg aaagaagcta
     3781 aagacctggt agaatctgca ccggctgctc tgaaagaagg cgtgagcaaa gacgacgcag
     3841 aagcactgaa aaaagctctg gaagaagctg gcgctgaagt tgaagttaaa taagccaacc
     3901 cttccggttg cagcctgaga aatcaggctg atggctggtg actttttagt caccagcctt
     3961 tttgcgctgt aaggcgccag tagcgtttca cactgtttga ctactgctgt gcctttcaat
     4021 gcttgtttct atcgacgact taatatactg cgacaggacg tccgttctgt gtaaatcgca
     4081 atgaaatggt ttaagcgtga tagcaacagg cattgcggaa agtgttccat tttccggtca
     4141 acaaaatagt gttgcacaaa ctgtccgctc aatggacaga tgggtcgact tgtcagcgag
     4201 ctgaggaacc ctatggttta ctcctatacc gagaaaaaac gtattcgtaa ggattttggt
     4261 aaacgtccac aagttctgga tgtaccttat ctcctttcta tccagcttga ctcgtttcag
     4321 aaatttatcg agcaagatcc tgaagggcag tatggtctgg aagctgcttt ccgttccgta
     4381 ttcccgattc agagctacag cggtaattcc gagctgcaat acgtcagcta ccgccttggc
     4441 gaaccggtgt ttgacgtcca ggaatgtcaa atccgtggcg tgacctattc cgcaccgctg
     4501 cgcgttaaac tgcgtctggt gatctatgag cgcgaagcgc cggaaggcac cgtaaaagac
     4561 attaaagaac aagaagtcta catgggcgaa attccgctca tgacagacaa cggtaccttt
     4621 gttatcaacg gtactgagcg tgttatcgtt tcccagctgc accgtagtcc gggcgtcttc
     4681 tttgactccg acaaaggtaa aacccactct tcgggtaaag tgctgtataa cgcgcgcatc
     4741 atcccttacc gtggttcctg gctggacttc gaattcgatc cgaaggacaa cctgttcgta
     4801 cgtatcgacc gtcgccgtaa actgcctgcg accatcattc tgcgcgccct gaactacacc
     4861 acagagcaga tcctcgacct gttctttgaa aaagttatct ttgaaatccg tgataacaag
     4921 ctgcagatgg aactggtgcc ggaacgcctg cgtggtgaaa ccgcatcttt tgacatcgaa
     4981 gctaacggta aagtgtacgt agaaaaaggc cgccgtatca ctgcgcgcca cattcgccag
     5041 ctggaaaaag acgacgtcaa actgatcgaa gtcccggttg agtacatcgc aggtaaagtg
     5101 gttgctaaag actatattga tgagtctacc ggcgagctga tctgcgcagc gaacatggag
     5161 ctgagcctgg atctgctggc taagctgagc cagtctggtc acaagcgtat cgaaacgctg
     5221 ttcaccaacg atctggatca cggcccatat atctctgaaa ccttacgtgt cgacccaact
     5281 aacgaccgtc tgagcgcact ggtagaaatc taccgcatga tgcgccctgg cgagccgccg
     5341 actcgtgaag cagctgaaag cctgttcgag aacctgttct tctccgaaga ccgttatgac
     5401 ttgtctgcgg ttggtcgtat gaagttcaac cgttctctgc tgcgcgaaga aatcgaaggt
     5461 tccggtattc tgagcaaaga cgacatcatt gatgttatga aaaagctcat cgatatccgt
     5521 aacggtaaag gcgaagtcga tgatatcgac cacctcggca accgtcgtat ccgttccgtt
     5581 ggcgaaatgg cggaaaacca gttccgcgtt ggcctggtac gtgtagagcg tgcggtgaaa
     5641 gagcgtctgt ctctgggcga tctggatacc ctgatgcctc aggatatgat caacgccaag
     5701 ccgatttccg cagcagtgaa agagttcttc ggttccagcc agctgtctca gtttatggac
     5761 cagaacaacc cgctgtctga gattacgcac aaacgtcgta tctccgcact cggcccaggc
     5821 ggtctgaccc gtgaacgtgc aggcttcgaa gttcgagacg tacacccgac tcactacggt
     5881 cgcgtatgtc caatcgaaac ccctgaaggt ccgaacatcg gtctgatcaa ctctctgtcc
     5941 gtgtacgcac agactaacga atacggcttc cttgagactc cgtatcgtaa agtgaccgac
     6001 ggtgttgtaa ctgacgaaat tcactacctg tctgctatcg aagaaggcaa ctacgttatc
     6061 gcccaggcga actccaacct ggatgaagga ggccacttcg tagaagacct ggtaacttgc
     6121 cgtagcaaag gcgaatccag cttgttcagc cgcgaccagg ttgactacat ggacgtatcc
     6181 acccagcagg tggtatccgt cggtgcgtcc ctgatcccgt tcctggaaca cgatgacgcc
     6241 aaccgtgcat tgatgggtgc gaacatgcaa cgtcaggccg ttccgactct gcgcgctgat
     6301 aagccgctgg ttggtactgg tatggaacgt gctgttgccg ttgactccgg tgtaactgcg
     6361 gtagctaaac gtggtggtgt cgttcagtac gtggatgctt cccgtatcgt tatcaaagtt
     6421 aacgaagacg agatgtatcc gggtgaagca ggtatcgaca tctacaacct gaccaaatac
     6481 acccgttcta accagaacac ctgtatcaac cagatgccgt gtgtgtctct gggtgaactg
     6541 gttgaacgtg gcgacgtgct ggcagacggt ccgtccaccg acctcggtga actggcgctt
     6601 ggtcagaaca tgcgcgtagc gttcatgccg tggaatggtt acaacttcga agactccatc
     6661 ctcgtatccg agcgtgttgt tcaggaagac cgtttcacca ccatccacat tcaggaactg
     6721 gcgtgtgtgt cccgtgacac caagctgggt ccggaagaga tcaccgctga catcccgaac
     6781 gtgggtgaag ctgcgctctc caaactggat gaatccggta tcgtttacat tggtgcggaa
     6841 gtgaccggtg gcgacattct ggttggtaag gtaacgccga aaggtgaaac tcagctgacc
     6901 ccagaagaaa aactgctgcg tgcgatcttc ggtgagaaag cctctgacgt taaagactct
     6961 tctctgcgcg taccaaacgg tgtatccggt acggttatcg acgttcaggt ctttactcgc
     7021 gatggcgtag aaaaagacaa acgtgcgctg gaaatcgaag aaatgcagct caaacaggcg
     7081 aagaaagacc tgtctgaaga actgcagatc ctcgaagcgg gtctgttcag ccgtatccgt
     7141 gctgtgctgg tagccggtgg cgttgaagct gagaagctcg acaaactgcc gcgcgatcgc
     7201 tggctggagc tgggcctgac agacgaagag aaacaaaatc agctggaaca gctggctgag
     7261 cagtatgacg aactgaaaca cgagttcgag aagaaactcg aagcgaaacg ccgaaaaatc
     7321 acccagggcg acgatctggc accgggcgtg ctgaagattg ttaaggtata tctggcggtt
     7381 aaacgccgta tccagcctgg tgacaagatg gcaggtcgtc acggtaacaa gggtgtaatt
     7441 tctaagatca acccgatcga agatatgcct tacgatgaaa acggtacgcc ggtagacatc
     7501 gtactgaacc cgctgggcgt accgtctcgt atgaacatcg gtcagatcct cgaaacccac
     7561 ctgggtatgg ctgcgaaagg tatcggcgac aagatcaacg ccatgctgaa acagcagcaa
     7621 gaagtcgcga aactgcgcga attcatccag cgtgcgtacg atctgggcgc tgacgttcgt
     7681 cagaaagttg acctgagtac cttcagcgat gaagaagtta tgcgtctggc tgaaaacctg
     7741 cgcaaaggta tgccaatcgc aacgccggtg ttcgacggtg cgaaagaagc agaaattaaa
     7801 gagctgctga aacttggcga cctgccgact tccggtcaga tccgcctgta cgatggtcgc
     7861 actggtgaac agttcgagcg tccggtaacc gttggttaca tgtacatgct gaaactgaac
     7921 cacctggtcg acgacaagat gcacgcgcgt tccaccggtt cttacagcct ggttactcag
     7981 cagccgctgg gtggtaaggc acagttcggt ggtcagcgtt tcggggagat ggaagtgtgg
     8041 gcgctggaag catacggcgc agcatacacc ctgcaggaaa tgctcactgt taagtctgat
     8101 gacgtgaacg gtcgtaccaa gatgtataaa aacatcgtgg acggcaacca tcagatggag
     8161 ccgggcatgc cagaatcctt caacgtattg ttgaaagaga ttcgttcgct gggtatcaac
     8221 atcgaactgg aagacgagta attctcgctc aaacaggtca ctgctgtcgg ggtaaaaccc
     8281 ggcagcggat tgtgctaact ccgacgggag caaatccgtg aaagatttat taaagtttct
     8341 gaaagcgcag actaaaaccg aagagtttga tgcgatcaaa attgctctgg cttcgccaga
     8401 catgatccgt tcatggtctt tcggtgaagt taaaaagccg gaaaccatca actaccgtac
     8461 gttcaaacca gaacgtgacg gccttttctg cgcccgtatc tttgggccgg taaaagatta
     8521 cgagtgcctg tgcggtaagt acaagcgcct gaaacaccgt ggcgtcatct gtgagaagtg
     8581 cggcgttgaa gtgacccaga ctaaagtacg ccgtgagcgt atgggccaca tcgaactggc
     8641 ttccccgact gcgcacatct ggttcctgaa atcgctgccg tcccgtatcg gtctgctgct
     8701 cgatatgccg ctgcgcgata tcgaacgcgt actgtacttt gaatcctatg tggttatcga
     8761 aggcggtatg accaacctgg aacgtcagca gatcctgact gaagagcagt atctggacgc
     8821 gctggaagag ttcggtgacg aattcgacgc gaagatgggt gcggaagcaa tccaggctct
     8881 gctgaagagc atggatctgg agcaagagtg cgaacagctg cgtgaagagc tgaacgaaac
     8941 caactccgaa actaagcgta aaaagctgac caagcgtatc aaactgctgg aagcgttcgt
     9001 tcagtctggt aacaaaccag agtggatgat cctgaccgtt ctgccggtac tgccgccaga
     9061 tctgcgtccg ctggttccgc tggatggtgg tcgtttcgcg acttctgacc tgaacgatct
     9121 gtatcgtcgc gtcattaacc gtaacaaccg tctgaaacgt ctgctggatc tggctgcgcc
     9181 ggacatcatc gtacgtaacg aaaaacgtat gctgcaggaa gcggtagacg ccctgctgga
     9241 taacggtcgt cgcggtcgtg cgatcaccgg ttctaacaag cgtcctctga aatctttggc
     9301 cgacatgatc aaaggtaaac agggtcgttt ccgtcagaac ctgctcggta agcgtgttga
     9361 ctactccggt cgttctgtaa tcaccgtagg tccatacctg cgtctgcatc agtgcggtct
     9421 gccgaagaaa atggcgctgg agctgttcaa accgttcatc tacggcaagc tggaactgcg
     9481 tggtcttgct accaccatta aagctgcgaa gaaaatggtt gagcgcgaag aagctgtcgt
     9541 ttgggatatc ctggacgaag ttatccgcga acacccggta ctgctgaacc gtgcaccgac
     9601 tctgcaccgt ctgggtatcc aggcatttga accggtactg atcgaaggta aagctatcca
     9661 gctgcacccg ctggtttgtg cggcatataa cgccgacttc gatggtgacc agatggctgt
     9721 tcacgtaccg ctgacgctgg aagcccagct ggaagcgcgt gcgctgatga tgtctaccaa
     9781 caacatcctg tccccggcga acggcgaacc aatcatcgtt ccgtctcagg acgttgtact
     9841 gggtctgtac tacatgaccc gtgactgtgt taacgccaaa ggcgaaggca tggtgctgac
     9901 tggcccgaaa gaagcagaac gtctgtatcg ctctggtctg gcttctctgc atgcgcgcgt
     9961 taaagtgcgt atcaccgagt atgaaaaaga tgctaacggt gaattagtag cgaaaaccag
    10021 cctgaaagac acgactgttg gccgtgccat tctgtggatg attgtaccga aaggtctgcc
    10081 ttactccatc gtcaaccagg cgctgggtaa aaaagcaatc tccaaaatgc tgaacacctg
    10141 ctaccgcatt ctcggtctga aaccgaccgt tatttttgcg gaccagatca tgtacaccgg
    10201 cttcgcctat gcagcgcgtt ctggtgcatc tgttggcatc gatgacatgg tcatcccgga
    10261 gaagaaacac gaaatcatct ccgaggcaga agcagaagtt gctgaaattc aggagcagtt
    10321 ccagtctggt ctggtaactg cgggcgaacg ctacaacaaa gttatcgata tctgggctgc
    10381 ggcgaacgat cgtgtatcca aagcgatgat ggataacctg caaactgaaa ccgttattaa
    10441 ccgtgacggt caggaagaga agcaggtttc cttcaacagc atctacatga tggccgactc
    10501 cggtgcgcgt ggttctgcgg cacagattcg tcagcttgct ggtatgcgtg gtctgatggc
    10561 aaagccggat ggctccatca tcgaaacgcc aatcaccgcg aacttccgtg aaggtctgaa
    10621 cgtactccag tacttcatct ccacccacgg tgctcgtaaa ggtctggcgg ataccgcact
    10681 gaaaactgcg aactccggtt acctgactcg tcgtctggtt gacgtggcgc aggacctggt
    10741 ggttaccgaa gacgattgtg gtacccatga aggtatcatg atgactccgg ttatcgaggg
    10801 tggtgacgtt aaagagccgc tgcgcgatcg cgtactgggt cgtgtaactg ctgaagacgt
    10861 tctgaagccg ggtactgctg atatcctcgt tccgcgcaac acgctgctgc acgaacagtg
    10921 gtgtgacctg ctggaagaga actctgtcga cgcggtaaaa gtacgttctg ttgtatcttg
    10981 tgacaccgac tttggtgtat gtgcgcactg ctacggtcgt gacctggcgc gtggccacat
    11041 catcaacaaa ggtgaagcaa tcggtgttat cgcggcacag tccatcggtg aaccgggtac
    11101 acagctgacc atgcgtacgt tccacatcgg tggtgcggca tctcgtgcgg ctgctgaatc
    11161 cagcatccag gtgaaaaaca aaggtagcat caagctcagc aacgtgaagt cggttgtgaa
    11221 ctccagcggt aaactggtta tcacttcccg taacaccgaa ctgaaactga tcgacgaatt
    11281 cggtcgtacc aaagaaagct acaaagtgcc ttacggtgcg gtactggcga aaggcgatgg
    11341 cgaacaggtt gctggcggcg aaaccgttgc aaactgggac ccgcacacca tgccggttat
    11401 caccgaagta agcggttttg tacgctttac tgacatgatc gacggccaga ccattactcg
    11461 tcagaccgac gaactgaccg gtctgtcttc gctggtggtt ctggattccg cagaacgtac
    11521 cgcaggtggt aaagatctgc gtccggcact gaaaatcgtt gatgctcagg gtaacgacgt
    11581 tctgatccca ggtaccgata tgcctgcgca gtacttcctg ccgggtaaag cgattgttca
    11641 gctggaagat ggcgtacaga tcagctctgg tgacaccttg gcgcgtattc cgcaggaatc
    11701 cggcggtacc aaggacatca ccggtggtct gccgcgcgtt gcggacctgt tcgaagctcg
    11761 tcgtccgaaa gaaccggcaa tcttggctga aatcagcggt atcgtttcct tcggtaaaga
    11821 aaccaaaggt aaacgtcgtc tggttatcac cccggtagac ggtagcgatc cgtacgaaga
    11881 gatgattccg aaatggcgtc agctcaacgt gttcgaaggt gaacgtgtag aacgtggtga
    11941 cgtaatttcc gacggtccgg aagcgccgca cgacattctg cgtctgcgtg gtgttcatgc
    12001 tgtgactcgt tacatcgtta acgaagtaca ggacgtatac cgtctgcagg gcgtaaagat
    12061 taacgataaa cacatcgaag ttatcgttcg tcagatgctg cgtaaagcta ccatcgttaa
    12121 cgcgggcagc tccgacttcc tggaaggcga acaggttgaa tactctcgcg tcaagatcgc
    12181 aaaccgcgaa ctggaagcga acggcaaagt gggtgcaact tactcccgcg atctgctggg
    12241 tatcaccaaa gcgtctctgg caaccgagtc cttcatctcc gcggcatcgt tccaggagac
    12301 cactcgcgtg ctgaccgaag cagccgttgc gggcaaacgc gacgaactgc gcggcctgaa
    12361 agagaacgtt atcgtgggtc gtctgatccc ggcaggtacc ggttacgcgt accaccagga
    12421 tcgtatgcgt cgccgtgctg cgggtgaagc tccggctgca ccgcaggtga ctgcagaaga
    12481 cgcatctgcc agcctggcag aactgctgaa cgcaggtctg ggcggttctg ataacgagta
    12541 atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat gggtgttatt
    12601 cagagggcaa attcttcacg gaaaatatta agaaaggcag ggcgaaacga ggaagaagct
    12661 tttcgccctg gacaacattc ctgctgacgg cactaccata aaaaaacaca acgctttcaa
    12721 caaccttgcg tttttcatct tccgtagcgg ctcggaataa atttacgttc ttacattccg
    12781 tctcatagtc tttgcgaggc gcgtcccaga tagctgtccc agtctttcca taccggctgc
    12841 aaaccctgag cggttaacgc ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag
    12901 aactgttcca actcggggtg attatcggca tagccacctg gctgcgtttt cgagaaggcg
    12961 ctgacgttat tgatcgccag cggaataacg cgatcgcgaa accacggtga ttcccgcgtg
    13021 gagagtgaca gttcaatctc cggtgcaagc agtcggaagg cgcagatggt ttgcactaac
    13081 tggcgttcat ccataatcga cgcaggctca atgccgccag tacacgggcg caggcgcgga
    13141 aaggagacag agtaacggct ttgccagtaa tgctgttgca gccatagcaa atgttcggca
    13201 accatatagc agtcaacgcg ccagttgtcg gaaaggccaa ttagcgcgcc gaggcctatc
    13261 ttatcaatcc ccgcacgccc cagccgatcc ggcgtttcca gccgccagaa gaagtcctgt
    13321 tttttgcctt tcaaatgatg gcgggtataa gtcgcctcgt gatatgtctc ctgataaacc
    13381 atcaccccat ccagaccaag ttgctttaac tcggcgtatt ccgtctccgc cagcggttgc
    13441 acttccattt gtagtgaaga gaactgttca cgcagggcgg ggagatgacg acgaaagtaa
    13501 tccatcccca ctttcgcctg atgttcacca gtgactaata gcagatgttc aaagcccatc
    13561 tcccgtatag cggcgctttc cctggcaata tccgcttcat ccagcgtttt gcgcttgatg
    13621 cgattactca tggaaaatcc gcagtacgtg cagttgttag cgcaaagatt ggaaagataa
    13681 agcgggacgt agaaactaac tgtgttgcca aatcgctgac gggtcagacg ctgcgcctgt
    13741 tgggccagtt gttccagata gccactggcg gcaggcgata acagcgccat catgtcgtcg
    13801 cgggtgagtt gcgaggcatt tagcgcccgc tctacgtcag cagccgtttt gccgttgata
    13861 cgcaggcgga tgtcgtccca gtccagttgt cgccagcgat tgctgaaggt tttcatgccg
    13921 atgcctccag aaatccggtc agcgggctgg tggcatgagc aaaataactg cggctgcccg
    13981 gtccggactg acgtgccagc aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt
    14041 tgacgggatc gtccgcgacg gcaatcgccg tattcactaa caccgcgtcg gcccccattt
    14101 ccagcgcctg cgcggcatgg ctgggaacgc cgatgccagc atcgacaacc accggaactg
    14161 tggcctgctg gataataatc tccagcatgg cacgggtttc cagtccctga ttcgagccaa
    14221 tcggcgcgcc gagcggcatc accgctgcac agccgacttc ttccagacgt ttacacaata
    14281 ccggatcggc cccgcagtaa ggcagcacga caaatccctg ttgtaccagc gtttcggcgg
    14341 ctttcagggt ttcgatggga tcgggcaaca gccagcgggc gtcagggtga atctccaatt
    14401 ttagccagtt tgtgcctaac gcttcacgtg ccagatgggc ggcgaaaatg gcttcttccg
    14461 ctgttttcgc cccggaagta ttcggcagca gggccacacc cgccgcgata agcggttcga
    14521 ggatagcgtc gttgtgctgg cgcaagtcga cacgtttcat cgccagtgtc accagctggc
    14581 tgccggaagc gcggatcgcc tccaccatca gttgtgaaga agcgaatttc cctgtgccgg
    14641 taaacagatg tgaatcaaac gttttgtcca caatacgtaa catctcaacc ccctgcaata
    14701 acctgaaaaa gcaggatctg gtcgccatcc tgcacgatat gttgcgtcca ctgctcacgc
    14761 gggacgattt gctgattaat cgccagagcc gcgcccgctt gtcgttggtc gagttgctcc
    14821 agtagttcgt gaacagtttg cccggcggcg cactgcatcg gttgatcgtt aaacaggatc
    14881 tgcattgctt cctccgcata ccgggcaacc actggcgcgg cgcaacgcca ggctgcgcca
    14941 ctggctcgac ttaccgtcga acagtcggag ttctcccgca ggcgtctcta taccgcttaa
    15001 taacttaatg gcttccagtg cctgcaaagt gcccatcacc ccgaccaccg ggccaaccac
    15061 gcccgccgtg cggcagttgc gttctggctg ctggttatct ggccacaggc agcggtaaca
    15121 cccctgctcc cagggcggtg tcagtaccat caactgaccg ccaaatccga ccgcgctggc
    15181 ggtgataagc ggcgtgttga gtgccacgca ggcggcatta atctcctggc gagtcgccat
    15241 attgtcggta cagtcgagca ccacatcggc ttgtgcaacc gcatctttca acgcctcacc
    15301 cgttaaccgt tgttgtaatg ctgtcagttg aatgtcggga ttcaactgtg tcagtcgctg
    15361 ttggctgacc tgcgatttcg ggctgttgat atctgcagtg gtaaagagga tttgtcgttg
    15421 cagattgctt aaatgcacat cgtcgtcatc tgccagtacc agcgtcccga cgccagcccc
    15481 tgccaggtat agcgcagcag gtgtacccag cccgccaaga ccaataatca gcacctggct
    15541 gtcgagcagt ttttgctgcc cgtcaagagc gatatcgtcg agcagaattt ggcggctata
    15601 acgcataaag tcacggtcat tcatcgccaa ctcctgcaat ttccagcaac tgtgccgttg
    15661 ccaaacgcca gtctgcggct tgagtaatgg cgctgacgac ggcgatactg ccgacacccg
    15721 ttgctatcac cgcaggcgcg cgtgccagac tgataccgcc aatcgccacg gtgggataat
    15781 ccgccaggcg ctcaacatgc cgtgccaact gttccagccc ctgcggtgca gaaggcatct
    15841 gtttggtttg cgtcgggaac acatgtccca gcgcgatata agaggggcgt gctgccagcg
    15901 cgacgtcgat ttccatatcg tcatgtgtcg aaacgcccag ccgcaggcct gccgcgcgga
    15961 tggcattgag atcggtggct tgcaaatctt cctgccccaa atggacgcca tacgcctgat
    16021 gcttgatcgc gagccgccag taatcgttga taaacaatcg cgcgttatag cggcggccca
    16081 gcgcaattgc cgccacgaca tcggcttcca cctcttcatc gcgccgatct ttgatgcgta
    16141 gctggagagt acgtacgcct gcatccaaca gacgttcgat ccactgtacg ctgtccacca
    16201 ccgggtacag tcctaaacga aaaggtacag gaggaaaatc aggctgatac atcacgcttc
    16261 ctccttatgc aggtagattt ctccgcctct ggcacggaag ttctccgaca tatccgccat
    16321 tcccacttca atggtttgcg cggcggcgta atcgcgtact tcctggctga ttttcatcga
    16381 gcagaatttt ggcccacaca tggagcaaaa atgggcgact ttgccggact cttggggcag
    16441 ggtttcatcg tgataagcgc gggcggtaaa cgggtcgagg gccagattaa actggtcttc
    16501 ccagcgaaat tcgaagcggg ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg
    16561 cccttttgcc aggtcagcag cgtgggcggc aatcttataa gtgataagcc cctgcttaac
    16621 atcttcttta ttgggcagac ccagatgctc ttttggcgtt acgtaacaga gcatcgcgca
    16681 gccaaaccag ccaatcatcg ccgcaccaat ccccgacgtg aagtggtcat agcccggtgc
    16741 aatatcggta gttagcggcc ccagagtgta aaacggcgct tcgtggcagt gctctaactc
    16801 ctcggtcata ttgcggtgga tcatctgcat cggcacatgg cctgggcctt caatcatcac
    16861 ctgtacgtca tattcccagg cgattttggt cagttcgccc agcgtatgca attcggcaaa
    16921 ctgcgcttca tcgttggcgt cctgaataga accggggcgc agaccgtcgc ccagcgacag
    16981 cgaaacgtca taagcggcac agatttcaca aatttcgcgg aagtgttgat agaggaaatt
    17041 ttcctgatga tgggagaggc accatttcgc cataatcgaa ccgccgcgag agacgatacc
    17101 ggtcaggcgt ttcgcggtca tcggcacata gcgcagcagt acgcccgcat ggatagtgaa
    17161 gtaatccaca ccttgctcgg cctgttccag cagcgtgtcg cggaacgctt cccaggtaag
    17221 atcttcggcg atcccgttaa ccttctccag cgcctggtag atcggcactg taccgatcgg
    17281 caccggactg ttacgcaaaa tccactcgcg ggtttcgtga atatagcgac cggtggagag
    17341 atccatcacc gtatccgctc cccagcgcgt ggaccatacc agcttttcca cttcttcttc
    17401 gatggaagag gtgaccgccg agttgccgat attggcgtta acttttacca ggaagttacg
    17461 gccaataatc atcggctccg attccggatg attaatgttg gccgggataa tcgcacgtcc
    17521 ggcagcaact tcatcacgga caaattccgc agtgatattt tccggcagat gtgcgccaaa
    17581 gctcattccc ggatgctggt ggcgtaaaac ctcgctacgg atgcgctcgc ggcccatatt
    17641 ctcgcggatg gcgatgaatt ccatttccgg cgtgatgatg ccctggcggg cgtagtgcag
    17701 ttgggtgaca cggcgtcctg ttttggcgcg ttttggcgtt aataagccgc taaaacgtag
    17761 ctcgtcgaga ccatcatctg ccagccgcgt tctggtgtaa tcggaactgc gcacggtaag
    17821 ttcttcggta tcggcgcgcg catcgatcca cggctggcgt agttttgcca gcccttgctg
    17881 cacgttaatg gcgatctgcg gatcaccata cgggccggag gtgtcgtaga ccggaatcgc
    17941 ttcgttttct tcgtactgcg gctgttcttt gctaccgcca attagcgtcg ggctaagctg
    18001 gatctcacgc atcggcacgc gcacgccggg gtgtgtgcca gtgatataaa tgcgttttga
    18061 gttgggaaag gcggtgcctt ccagggtgtc gataaaatgt tgggcccggg cgcgttgttc
    18121 gcggcgggtc agttttgttg cagacatagc tcattccaaa aagttaagga cgtggcttgt
    18181 cagacgacgg atgaagcaag agacgatcgc cgcaaaggcg atgcgatagc agattaactc
    18241 ttgttccctt cgcaggtatt agcctgatca ggttccgcgg atcccgaata aacggtctca
    18301 gccagttaag gcactccgac aagaaattag cccccacaaa cggggcgttg aatgtaaatt
    18361 acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc
    18421 agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat gtcagataaa
    18481 acctgttgaa actcaaggta attatcatga tcgatggcgg tttccaggct ggaatcgtag
    18541 taatccataa tctgttgggt attggcttcg agttgcggcc aaatcttcgc ggcgcgcgcg
    18601 agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa atgtccggca
    18661 gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg
    18721 tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag cagatgctta
    18781 cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta
    18841 tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc tcagtgagaa
    18901 atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc tatggatcgt
    18961 ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa attatggttg
    19021 ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca
    19081 ctacaaatcg gcgaatggca gggggaacct gtttggttaa tacaacagca gcggcgtcac
    19141 gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca actggccgga
    19201 cgtggcgtgc aactggcgga gttttaccga tcgcataaat actgtggtta ctgcgggcat
    19261 gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg tgagcgttac
    19321 tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc gatcctcctc
    19381 gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt cgtcgaagtg
    19441 ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg aattaaagtt
    19501 aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg
    19561 tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg
    19621 aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt
    19681 ctgatagaag atactgtggc gatgtgtcgg gcagagtatg agtgatgata cactgaccgc
    19741 ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg
    19801 ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc gggtcgctat
    19861 ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac
    19921 gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg
    19981 atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct ctattttgaa
    20041 gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt cgataaactg
    20101 ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg taccattcgt
    20161 cacgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg gacgctggca
    20221 acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat
    20281 gccgatccgc aggcgctgca cgctctgctc gataaactgg cgaaaagcgt cactttgtat
    20341 ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac ctggggcggc
    20401 gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa aattgttgat
    20461 ggtttactgc gtgaaaacga cggtcgccgc gtgccagtca cgctgtttac caaaggcggc
    20521 ggacagtggc tggaagccat ggctgaaacc ggttgcgatg cgctgggcct cgactggaca
    20581 acggacatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca gggtaatatg
    20641 gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc gactatactt
    20701 gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat tcatcaggat
    20761 gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc tgaacagtat
    20821 caccgctaag agcctgatcc atcaggctat tttatttgcc attttgaggc cgggcagtgc
    20881 tcaaaatcct cacgtactac gtgtacgctc cggtttttcc gcgctgtccg gcctcaaact
    20941 gccttcaaca attacgcctg ttgaaccagg ctctaaggag cgattatgga tctcgcgtca
    21001 ttacgcgctc aacaaatcga actggcttct tctgtgatct gcgaggatcg actcgataaa
    21061 gatccaccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg agaagtgacg
    21121 cgagcggcga tggtgctgct gaaatatccc tcgcttgagc tggtggagta taaagttgcc
    21181 cgcatcgcca ccaccatgcc ttacattcca ggttttcttt ccttccgcga atatcctgcg
    21241 ctgctggcag cgtgggagat gctgtcgcaa aagccggatt tagtgtttgt cgatggtcat
    21301 gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggcttatt ggtggatgtg
    21361 ccgaccattg gcgtggcgaa aaaacggctc tgcggtaaat tcgaaccgct ctccagcaaa
    21421 ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt ctggcgcagc
    21481 aaagcgcgct gtaacccgtt gtttatcgct accggccatc gggtcagcgt ggacagcgcg
    21541 ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac gcgctgggcg
    21601 gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca gccctaattc
    21661 aggtaaactg cggataattt ccgtatttga gaactcaaca tgttacaaaa cccaattcat
    21721 ctgcgtctgg agcgcctgga aagctggcag catgtcactt tcatggcttg cttatgcgaa
    21781 cgcatgtacc ccaattacgc catgttctgc cagcaaaccg gttttggtga tgggcaaatt
    21841 taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagacgc aaaagtaaat
    21901 ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga tttcgatctg
    21961 tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt ccattcgcgt
    22021 ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat cacgaccgtt
    22081 gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg atgaagagct taaagaaaac
    22141 ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt agctgagtgc
    22201 gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga gtcgggtgag
    22261 agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa cgcctgattt
    22321 gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg ggcgaaaaaa
    22381 agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt cgcactcgat
    22441 gcttagcaag cgataaacac attgtaagga taacttatga acaagactca actgattgat
    22501 gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct ggagtccact
    22561 ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt tggtttcggt
    22621 accttcaaag tgaaccatcg cgctgagcgt actggccgca acccgcagac cggtaaagaa
    22681 atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact gaaagacgca
    22741 gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc accccttttg
    22801 tctttctggc gtcgatcatt gatgctggca ggtgcgctgt ttctcactgc ctgtagccac
    22861 aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg cgcggtacgc
    22921 atctggcgaa aagacagcgg cgataatgtg catctgcttg ccgtgtttag cccgtggcgc
    22981 agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac gctcatcaat
    23041 atcaatgttt acagcaaacc gccggtgaac attcgcgcga gttttgacga tcgcggcgat
    23101 ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa cgaccaaatc
    23161 gatttatacc gttatcgtgc tgatcagatc cgccagatta gcgatgcctt acgtcagggg
    23221 agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac cacctgcgaa
    23281 gggcaaacca ttaaacctga tttagattcg caggcgatag cgcatatcga gcgccgccag
    23341 agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg ttcgcaatta
    23401 ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac gttctgattt
    23461 accagtggcc catacccata tgaccgccac caccgcagcc gccgtagccc atgcccattc
    23521 cggctccgcg cggaataccc gcttcagcca tcgcgatatc tcgtttcacc cgtaactcat
    23581 ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg ctgctatccg
    23641 gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt tgctgttgca
    23701 gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc tgttgttcgc
    23761 tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca aatgcagatg
    23821 tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc gtgttccgtt
    23881 tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg tgccaacgat
    23941 gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac tcgcctgctg
    24001 cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag cgcagggtaa
    24061 gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg caaagaagaa
    24121 gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg cgatcctccc
    24181 cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg attatgggcg
    24241 cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta ttcgcgctct
    24301 tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc agcaggcctt
    24361 actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg atgaacaagg
    24421 aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt cccctcagga
    24481 aatgcagcag ttacatccgg gagatgaaga agcgtggcgg cggatcgata gcgcagacgg
    24541 cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg gaatgcaccg
    24601 gatgcgccat atgcagcagt atgccgcgac accacaagca attttcatcg ctttcgacgc
    24661 cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta ttctcttcgc
    24721 cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc gctatctgcg
    24781 ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg cgctggggca
    24841 tcttgcggca ggcgttgccc acgaaatccg taacccactt tcctcgatta aaggactggc
    24901 gaaatacttt gccgagcgcg cgcctgcagg gggagaagcg catcaactgg cgcaggtgat
    24961 ggcgaaagag gccgaccgtt taaaccgcgt ggtaagcgag ttgctggaac tggttaagcc
    25021 aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact cattacagct
    25081 ggtaagccag gatgcaaaca gccgggagat ccagttacgc tttaccgcca acgacacatt
    25141 accggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc tctatctcaa
    25201 tgctattcag gcgattggtc agcatggcgt gattagcgtg acggccagcg aaagcggcgc
    25261 gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc agcttgaagc
    25321 catcttcact ccgtacttca ccaccaaagc cgaaggcacc ggattggggc tggcggtcgt
    25381 gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagccagg agggaaaagg
    25441 ctcaacgttc accctctggc ttccggtcaa tattacgcgt aaggacccac aaggatgacg
    25501 cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac tattttgcag
    25561 gctttactgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg acaggcgctg
    25621 gagcaggtgc gggaacaggt ttttgatctt gtgctttgcg atgtgcgaat ggcggagatg
    25681 gacggcatcg ccacgctgaa agagatcaaa gcgttaaacc cggcaattcc ggtgctgatt
    25741 atgactgcgt actccagcgt cgagacggcg gtagaggcac tgaaaactgg ggcgctggat
    25801 tatctcatca agccgctgga tttcgataac ctacaggcga cgctggaaaa ggcgctcgcg
    25861 catacgcaca gtattgatgc tgaaacacct gcggtgactg ccagccagtt cggtatggtc
    25921 ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc gccatcggaa
    25981 gccacggtgc tgatccacgg cgattccggc accggtaaag agctggtcgc cagggcgatt
    26041 cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc ggcactcaac
    26101 gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac tggagccgat
    26161 aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgctgtttct cgatgaaatt
    26221 ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg cgattcagga gcgcgaagtt
    26281 cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc ggctgattgc ggcgactcat
    26341 cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta ctatcgcctg
    26401 aatgtggtgg cgattgaagt accatcgctg cggcaacggc gggaagatat ccctctgctg
    26461 gctggccatt ttcttcagcg ctttgccgag cgtaatcgca aggcggtaaa aggctttacg
    26521 ccacaggcga tggatttgct gatccactac gactggccgg gaaatattcg tgaactggaa
    26581 aacgcggtgg aacgggcggt ggtgctgctg accggggaat atatttccga acgcgagctg
    26641 ccgctggcga ttgccagtac gccgatcccg ctggggcaaa gtcaggatat tcagccgctg
    26701 gtggaagtgg aaaaagaggt gattctggcg gcgctggaga aaacgggcgg caacaaaacc
    26761 gaagccgccc gtcagttagg gatcacgcgc aaaacgctat tggcaaaact gtcgcgttag
    26821 ttctgctcgc gttcgatggc gcgccaaccg atatctttcc ggcagaagca atcatcccag
    26881 tggatatcgg tcattaaggc gtaggcgcgt ttctgcgctt ctgccacggt atgacccagc
    26941 gcggtgacgc acagtacgcg cccgccgctg gttaccacct gctcgtcatc cgccagtttt
    27001 gtgcccgcgt ggaacacttt gccgtcagcc acttcttcca gcggcaggcc gtggatcaca
    27061 tcgccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgct gagagaagca
    27121 cgttcatccc actctgacgt tttctcgtcc agtttgcctt cacaggccgc caggcagagc
    27181 tcaaccagat cggacttcat gcgcagcata atcggctggg tttccggatc gccaaagcgg
    27241 cagttaaatt caataacctt cggattgccc tgtttgtcga tcatcaggcc cgcatagaga
    27301 aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca cggttggcca gatgatacgt
    27361 tccatggtgc gctgatgaac ttcatcggtt actaccggcg cgggggagta agcgcccatc
    27421 ccgccggtgt ttggtccgtt atctttatcg cctacgcgtt tgtgatcctg gctggtagcc
    27481 atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc gccatcgagg
    27541 aactcttcga taacgatgcg atgacccgcg tcgccaaaag cgttgcccgc cagcatatcg
    27601 tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt cccggcagcc
    27661 agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacac cagcgcaggt
    27721 tctacctcgg tgaagttctg gtattccgcc gtagggatct tatggcgggc caggaaatct
    27781 ttggtaaacg cttttgagcc ttccagttgg gccgcacctg cggttgggcc gaagattttc
    27841 agcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc cgggccgacg
    27901 atggtcagat caatcttttc gttttgtgcg aaatccaaca gcgccgggat atcggtcacg
    27961 ccgatagcaa cgttttgcag cgcgggttcc agcgcagtgc ctgcattacc cggagcaaca
    28021 aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg ctcgcgcccg
    28081 ccgttaccaa tcactaatac tttcatctat tgctccatta atggcggaag tggcgcatgt
    28141 cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg tcatcacgga
    28201 tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca tcaataccgt
    28261 cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc aggccttcat
    28321 cggcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg cccgcgccaa
    28381 taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg aatttcgcca
    28441 ctttccagca gaacagcgca tcacgcagtt cctgttcggt cggctgacgt tgggtgacga
    28501 cgcgcagttc ttccgcaccg accatcccca ggtcacgatc ctgaaccagc agaccgccgt
    28561 tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag gtcagaacgc
    28621 gtacgttctg cttggcggcg gtgattttca gggcttcttc gctggcggaa ggcgcaataa
    28681 tcacttcgac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc agctcgcggt
    28741 taaaggcaat gatgccgccg aatgcggagg ttgggtcggt tttgtacgcg cgatcgtaag
    28801 catcaagaat ggaattgccg atagctacac cgcaagggtt ggcgtgcttc acaatcacac
    28861 atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg gcgatgttgt
    28921 tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct tctttcacat
    28981 tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc atatcctgct
    29041 tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc ggcggcttct ttgctttcac
    29101 cgtggtacgc cggaaccatg ctgccgaagt agttggcaat catgctgtcg taggcggcag
    29161 tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc gatccttcgt
    29221 tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg attgcgacat
    29281 ctttatggtt cttagcggcg gagcgcacca tggtcgggcc gccgatatcg atgttctcaa
    29341 ccgcatcttc cagcgagcag ccttcacgag caacagtctg ggcgaacgga tacaggttaa
    29401 caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg tcctggccgc
    29461 gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt ccatccatca
    29521 tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt tctgctaaca
    29581 gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt gcctgggcga
    29641 attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact ggacgacgtt
    29701 gttgcatggt aaatcccttg ggtttgacta ttacagagag cgttagctga atttttcgcg
    29761 aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat tgtaacgaaa
    29821 acgtttgcgc aacgctcgcg aatttttctc tttcaatggt gatcacaatt ttgacagtgg
    29881 ttaccgtggg aaaaatacag aaattacatt gatgattgtg gataactctg tgcgtaaaaa
    29941 ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg caatttttct
    30001 attgcggcct gcggagaact ccctataatg cgccttcatc gacacggcgg atgtgaatca
    30061 cttcacacaa acagccggtt cggttgaaga
//
LOCUS       DAJTRQ010000035        29934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000035 DAJTRQ010000000
VERSION     DAJTRQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            227..1675
                     /gene="uacT"
                     /locus_tag="OO332_001680"
     CDS             227..1675
                     /gene="uacT"
                     /locus_tag="OO332_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5942192.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1677..1802
                     /locus_tag="OO332_001681"
     CDS             1677..1802
                     /locus_tag="OO332_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942193.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1799..1870)
                     /locus_tag="OO332_001682"
     CDS             complement(1799..1870)
                     /locus_tag="OO332_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942194.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1925..2473
                     /gene="idi"
                     /locus_tag="OO332_001683"
     CDS             1925..2473
                     /gene="idi"
                     /locus_tag="OO332_001683"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5942195.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2516..4033)
                     /gene="lysS"
                     /locus_tag="OO332_001684"
     CDS             complement(2516..4033)
                     /gene="lysS"
                     /locus_tag="OO332_001684"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5942196.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4043..5141)
                     /gene="prfB"
                     /locus_tag="OO332_001685"
     CDS             complement(join(4043..5065,5067..5141))
                     /gene="prfB"
                     /locus_tag="OO332_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5942197.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5232..6965)
                     /gene="recJ"
                     /locus_tag="OO332_001686"
     CDS             complement(5232..6965)
                     /gene="recJ"
                     /locus_tag="OO332_001686"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5942198.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6971..7681)
                     /gene="dsbC"
                     /locus_tag="OO332_001687"
     CDS             complement(6971..7681)
                     /gene="dsbC"
                     /locus_tag="OO332_001687"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5942199.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7706..8602)
                     /gene="xerD"
                     /locus_tag="OO332_001688"
     CDS             complement(7706..8602)
                     /gene="xerD"
                     /locus_tag="OO332_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5942200.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8714..9235
                     /gene="fldB"
                     /locus_tag="OO332_001689"
     CDS             8714..9235
                     /gene="fldB"
                     /locus_tag="OO332_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5942201.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9275..9682)
                     /locus_tag="OO332_001690"
     CDS             complement(9275..9682)
                     /locus_tag="OO332_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5942202.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9663..9929)
                     /gene="sdhE"
                     /locus_tag="OO332_001691"
     CDS             complement(9663..9929)
                     /gene="sdhE"
                     /locus_tag="OO332_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5942203.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10172..11152
                     /gene="ygfZ"
                     /locus_tag="OO332_001692"
     CDS             10172..11152
                     /gene="ygfZ"
                     /locus_tag="OO332_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5942204.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11348..12007)
                     /locus_tag="OO332_001693"
     CDS             complement(11348..12007)
                     /locus_tag="OO332_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5942205.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12171..12482)
                     /locus_tag="OO332_001694"
     CDS             complement(12171..12482)
                     /locus_tag="OO332_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5942206.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12527..13959
                     /gene="bglA"
                     /locus_tag="OO332_001695"
                     /pseudo
     CDS             12527..13959
                     /gene="bglA"
                     /locus_tag="OO332_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
     gene            complement(14016..14759)
                     /locus_tag="OO332_001696"
     CDS             complement(14016..14759)
                     /locus_tag="OO332_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5942207.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15026..17899)
                     /gene="gcvP"
                     /locus_tag="OO332_001697"
     CDS             complement(15026..17899)
                     /gene="gcvP"
                     /locus_tag="OO332_001697"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5942208.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18018..18407)
                     /gene="gcvH"
                     /locus_tag="OO332_001698"
     CDS             complement(18018..18407)
                     /gene="gcvH"
                     /locus_tag="OO332_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5942209.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18431..19525)
                     /gene="gcvT"
                     /locus_tag="OO332_001699"
     CDS             complement(18431..19525)
                     /gene="gcvT"
                     /locus_tag="OO332_001699"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5942210.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19816..19899
                     /locus_tag="OO332_001700"
     CDS             19816..19899
                     /locus_tag="OO332_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942211.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19973..21175)
                     /gene="ubiI"
                     /locus_tag="OO332_001701"
                     /gene_synonym="visC"
     CDS             complement(19973..21175)
                     /gene="ubiI"
                     /locus_tag="OO332_001701"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5942212.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21198..22376)
                     /gene="ubiH"
                     /locus_tag="OO332_001702"
                     /gene_synonym="visB"
     CDS             complement(21198..22376)
                     /gene="ubiH"
                     /locus_tag="OO332_001702"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5942213.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22373..23698)
                     /gene="pepP"
                     /locus_tag="OO332_001703"
     CDS             complement(22373..23698)
                     /gene="pepP"
                     /locus_tag="OO332_001703"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5942214.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23724..24302)
                     /gene="ygfB"
                     /locus_tag="OO332_001704"
     CDS             complement(23724..24302)
                     /gene="ygfB"
                     /locus_tag="OO332_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5942215.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24470..24799
                     /gene="zapA"
                     /locus_tag="OO332_001705"
     CDS             24470..24799
                     /gene="zapA"
                     /locus_tag="OO332_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5942216.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24841..25024
                     /gene="ssrS"
                     /locus_tag="OO332_001706"
     ncRNA           24841..25024
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO332_001706"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25045..25647
                     /locus_tag="OO332_001707"
     CDS             25045..25647
                     /locus_tag="OO332_001707"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5942217.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25744..25803)
                     /locus_tag="OO332_001708"
     CDS             complement(25744..25803)
                     /locus_tag="OO332_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5942218.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26136..26195)
                     /gene="ibsC"
                     /locus_tag="OO332_001709"
     CDS             complement(26136..26195)
                     /gene="ibsC"
                     /locus_tag="OO332_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5942219.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26424..27656)
                     /gene="serA"
                     /locus_tag="OO332_001710"
     CDS             complement(26424..27656)
                     /gene="serA"
                     /locus_tag="OO332_001710"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5942220.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27912..28571)
                     /gene="rpiA"
                     /locus_tag="OO332_001711"
     CDS             complement(27912..28571)
                     /gene="rpiA"
                     /locus_tag="OO332_001711"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5942221.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28954..29847
                     /gene="argP"
                     /locus_tag="OO332_001712"
     CDS             28954..29847
                     /gene="argP"
                     /locus_tag="OO332_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5942222.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7113 a         8061 c         7505 g         7255 t
ORIGIN      
        1 gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca ttgtcaggtt
       61 ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat tcctgtctct
      121 aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa
      181 cgtaaacggt tgctttttat tctggcgggc gaaaggagaa acactgatga gcgccataga
      241 ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt
      301 atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc
      361 agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg ctattgcgat
      421 gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat
      481 cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac
      541 accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac
      601 tatcgccgcg ggttttatca ccacattatt agcgccactt attggtcgct tgatgccttt
      661 attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt
      721 gggtattgac tgggccgctg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt
      781 aggtatctcc tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt
      841 tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat
      901 gaatgaagtc aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat
      961 gtcgtttggt atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat
     1021 catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa
     1081 actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg
     1141 cggcacgttt aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt
     1201 gatgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg
     1261 catggtgcca aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc
     1321 tggtctagtg atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa
     1381 ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac
     1441 tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgctgctaca
     1501 tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aacgtcttct ttaatggcta
     1561 tcagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac
     1621 agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga
     1681 attttttaat gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca
     1741 tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt
     1801 aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctctctt
     1861 ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga
     1921 aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct
     1981 ggaaaagtat gccgcacaca cggcagacac cctcttacat ctcgcgttct ccagttggct
     2041 gtttaatgcc aaggggcaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc
     2101 tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca gcgaagacgc
     2161 ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta
     2221 tcctgacttt cgctaccgcg ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc
     2281 ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta
     2341 tcaatggtgt gatttagctg atgtattacg cggtattgat gccacgccgt gggcgttcag
     2401 tccgtggatg gtgatgcagg cgacaaatcg cgaagccaga atacgattat ctgcatttac
     2461 ccagcttaaa taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt
     2521 tcaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca
     2581 gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca
     2641 gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg
     2701 caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt
     2761 taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt
     2821 cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa
     2881 tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc
     2941 tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca
     3001 gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt
     3061 cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa
     3121 tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg
     3181 cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt
     3241 cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct
     3301 tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg
     3361 tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt
     3421 ccataaagcc acggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt
     3481 taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga cgatagcgcg
     3541 cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca
     3601 gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt
     3661 tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg
     3721 gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga
     3781 aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt
     3841 tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg
     3901 tatgatcgcg acggaaatcg ttcgggaagg caattccctg ctcacgcagg ttcgccagct
     3961 tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt
     4021 gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat
     4081 ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggtg cgcagatctt
     4141 taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg atgtcagatt
     4201 tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct
     4261 tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg tcgttctggc
     4321 actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg gtacggttaa
     4381 cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga
     4441 tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg aacgacgtgt
     4501 ggcgacgacc gccggagtca aacgggcttt tacgcaccag gcggtgaacg cccgtttctg
     4561 tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg
     4621 ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat tctgcccagc
     4681 gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg
     4741 cctgaatatc gaggtaacag tcggcgctgt catattcgcc agagaacata cggcggaact
     4801 caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt
     4861 cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt
     4921 tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct
     4981 gtgcgcgttc gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca
     5041 gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg
     5101 aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg
     5161 agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag
     5221 agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg
     5281 cgaaactcgt tgatatcgag cttataagcc agttgcactt cgcggacgcc gttatccggc
     5341 cagagagcgg tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt
     5401 tcgaccatca ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga
     5461 ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcagctgc
     5521 gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc
     5581 aatagcgaag gggccagcca ctcagtaacc agttcgccaa accgttgttg aaagagttcg
     5641 aatttatcct cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttgagcatc
     5701 atgccggggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag cccctgaatg
     5761 gagcgaccgg aacctttcag cgtaccgtca cctgctggcg caaaggcgat aaccggacgg
     5821 tgaaaacgct ctttgatacg cgaagccaga ataccgacaa cgccctgatg ccattcgggg
     5881 tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt ctcgcacagg
     5941 gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg
     6001 agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacacca
     6061 acggacatat catccagtcg cccggcagca ttgagacgcg gtcccagcgc aaaacctaaa
     6121 tcgctggcgg ctagtttttg tgcatcgcga tttgccactt ccagcagcgc tttaatccct
     6181 ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta
     6241 gcgtccagcg gcacgacgtc cgctactgtc cccagcgcta ccagatccag cagttctgcc
     6301 aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa aaaggtgcgc
     6361 agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta
     6421 cagtcgcgca agttagggtt aatgatcgct tccgctgcgg gtaatgtgtc gcccggcaaa
     6481 tggtgatcgg taacaataac cgggatgccc aacgagcgag cgtgctcaac tcccgcatgg
     6541 gaggaaatac cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg
     6601 accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata
     6661 ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg
     6721 gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc
     6781 tcaacggcct tttcgacacc gcttagttgc tgccagggca acataccttt aacactgcgt
     6841 tccagttctt gcgcactgcg tactccccgg ctggcgtata aacggcgcag caagggaggc
     6901 aattcagcgg gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt
     6961 ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatgt
     7021 ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac
     7081 cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg
     7141 ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac
     7201 accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa
     7261 gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc
     7321 agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat
     7381 aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat
     7441 tgaccggagc cgtgccacta acgtcataca ttggcccctg aatgatatgt ttaccatcat
     7501 cggtgatgta caacacaccg ctgttagtca gaactgtctt catgccagct acgggcgcgg
     7561 gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat
     7621 cagcctgagc aaagcctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca
     7681 taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc
     7741 gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca
     7801 gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt
     7861 gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg gcataatgtt
     7921 taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca
     7981 cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc
     8041 agtaaaccgc ctcttcccct aacggcacca ggcgctcttt gttgccttta ccaatgaccc
     8101 gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt tcagagacac
     8161 gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat
     8221 caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac
     8281 gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc
     8341 gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt
     8401 tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg
     8461 ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag
     8521 cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga
     8581 tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc
     8641 ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg
     8701 agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat
     8761 ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa
     8821 ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg
     8881 ggattttggt gaaatccaag aagactggga agccgtctgg gatcagctcg acgacctgaa
     8941 ccttgaaggt aaaattattg cgctgtatgg gcttggcgat caactgggat acggcgagtg
     9001 gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt
     9061 cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg
     9121 gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg
     9181 tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca
     9241 ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca
     9301 aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc agccataaat
     9361 gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg
     9421 gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc
     9481 gaatttcccc ctggcgagca ttaatacacc gctggctgcg aacgcaatca aataccacca
     9541 gcgaaagtaa caccatccat aacggggtgt aactgagcgg ccagggcatg agtaaaataa
     9601 cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca
     9661 aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccattatt
     9721 tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg
     9781 tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca
     9841 tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa
     9901 tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc
     9961 ggctattgta acgtgttttt cgacttcttt tacgggaata tcagtaaaca ccatcgcgat
    10021 cgcgaaatta attcacaaca attcaagggc ttcatttttt tggcagtcgc ctcgcagaag
    10081 gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt
    10141 aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc ctccacgtca
    10201 gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc
    10261 caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag
    10321 ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg
    10381 gagcaattta cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt
    10441 gcgtgaaccg cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc
    10501 gccagacgac gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc
    10561 aaatctcttt agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac
    10621 tttgctatgg tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc
    10681 taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct
    10741 tgcattaaac attgaagctg gtttcccggt gattgatgcc gccaacagcg ggcagtttat
    10801 cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac
    10861 cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aacaaacgcg cgctctggtt
    10921 gctggcaggt agtgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg
    10981 cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt
    11041 cgtggtacag gtcgtcatga ataacgatat ggaaccggac agcatcttcc gcgtacgcga
    11101 cgatgcgaat acactgcata tcgagccgct gccgtattcg ctcgaagagt aaatctctgt
    11161 atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt
    11221 gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgcatc
    11281 cggcggttgc gctccgatgc ctgatgcgac gctgacgcgt cttatcaggc ctacaaaggc
    11341 atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc
    11401 cgagcacgaa gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa
    11461 tcaccccgag cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga
    11521 gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc agataagtca
    11581 ccagagataa aattttgaat cggtgcgcga tggtcagttt gaacagaatc cccagcaatg
    11641 ctaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca
    11701 aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca
    11761 gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca
    11821 ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat
    11881 ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccagc ccaatgccgt
    11941 gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct
    12001 gaaccataac ttactccaga gaaacgtaca cgcacatgta tacctctcca gcgtaactga
    12061 gaatagttcc agtgaacacc tgttagctaa aataaatttt gatttgagac taatctccta
    12121 aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat ttaaagacat
    12181 ttaaattcaa tcatataaaa ttgtgtctga ccgggataga tgtcagcaat gacctttttc
    12241 agttcaggca gggtcatatt ttcctgctct gcatgttttt ccgtcagtgt atccagcgtt
    12301 acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg
    12361 acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt
    12421 ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc
    12481 atggtactat ttcctgtaag aattgactga tctggagcct atgatagtga aaaaactcac
    12541 cttaccgaaa gatttcttat ggggtggcgc cgttgccgct catcaggtcg aaggcggctg
    12601 gaacaaaggc ggcaaaggac cgagcatttg tgacgttctg accggtggcg cacacggcgt
    12661 gccgcgcgaa atcaccaaag aagtcttgcc aggaaaatac tatccaaacc atgaagccgt
    12721 tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg
    12781 ttttcggact tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa
    12841 cgaagaaggg ctgaagtttt acgactctct gttcgatgaa ctgctgaaat acaacatcga
    12901 accggtgata accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag
    12961 ctggaccaac cgtaaattcg ttgatttctt tgtccgtttc gcggaagtgg tattcgaacg
    13021 ctacaagcat aaagtcaaat actggatgac cttcaacgaa ataaacaacc agcgtaactg
    13081 gcgtgcgccg ctgtttggtt actgctgctc cggcgtggtg tataccgagc atgacaaccc
    13141 ggaagagacg atgtaccagg tgctgcatca ccagtttgtc gccagcgccc tggcagtgaa
    13201 agccgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct
    13261 ctatccctac tcatgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg
    13321 ctacgtcttt accgatgtgc agctatgcgg ctattacccg tcctatgtat tgaacgagtg
    13381 ggaacgtcgc ggatttaata tcaaaatgga agacggcgat ctcgatgtac tgcgcgaagg
    13441 cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg
    13501 caccggcgat gcgatctctg gttttgaagg cagcgtaccg aacccgtatg ttaaagcgtc
    13561 tgactggggc tgcagattga tccagtaggt ctgcgctatg ccctttgcga actgtatgag
    13621 cgttatcaga ggccgctgtt tattgtcgaa aacggttttg gcgcttacga caaagtggaa
    13681 gaagatggca gcatcaacga cgactaccgc attgactacc tgcgcgccca tattgaagag
    13741 atgaaaaaag cagtgactta cgatggtgtg gatctgatgg gctacacacc gtggggctgc
    13801 atcgactgcg tgtcattcac taccgggcag tacagcaaac gctacggctt tatctatgtg
    13861 aataaacatg acgacggtac tggcgatatg tcgcgttcac gtaagaagag ctttaactgg
    13921 tacaaagagg tgattgccag caacggcgag aagctttaag tcgatgaagt accggatgca
    13981 atacttgttg catccggtca gacaacttcc ctgttttatt tcccgcccgc caaatcgata
    14041 aaacttcccg tgacgtaaga ggctttatca cttagtagcc agacaatggc ctgcgcgacc
    14101 tcttctgcct gtccaccacg ctgcatgggg atgttcgact taacgcgatc gacgcgtcca
    14161 ggctcgccgc cgctggcgtg catttcggta taaataaacc ctggccgcac gcagttaacg
    14221 cggatcccct gcgcggcgac ttccagcgat cgtccggtgg ttaacgtatc aatcgcccct
    14281 ttcgatgccg cgtaatcaac atattcccct ggcgaaccca accgtgaggc caccgaagag
    14341 acattgacga tagcgccgcc actgccacca tttttaagcg ccatgcgttt taccgcctcg
    14401 cggcagcaga gaaaatatcc cgtcacgttg gtggaaagta ctcggttgat tcgctctgcg
    14461 gtaaggtttt caacggtgca ctgggtaaac aagatcccgg cgttattgac cagcgctgct
    14521 agcggttcat cgtgctgatc gattgctgta aacatcgcaa cgacctggtt ttcgtcgctg
    14581 atatccgcct ggagcacgaa tgctttgcca ccggcttgca ttattaagtt catcacttcc
    14641 tgcgccgcgt ggaggttttg ctgataatta accgccaccg tatacccttc ttgcgccaac
    14701 agtaatgcag ttgcccgccc gatgccgcga ctgccaccgg tcacaagtgc tatagccatt
    14761 tgtttttccg agaaaaatta attcgttgac gtatctttga gattgctaac aaactggctc
    14821 aatcttcaat gttggcacgc ttttccccct cgccctttca gggagatggt cggggtgagg
    14881 gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc ccaaaggggc
    14941 gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg ccgaagcgcc
    15001 tttagaaaat actcgaatca gtgaattact ggtattcgct aatcggtacg caggagcaga
    15061 acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag tatttgtctg
    15121 ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac tcggcgacca
    15181 gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc cagacaccgg
    15241 ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag cgatccagtt
    15301 ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc gggaacgaca
    15361 tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc tcgctgatgc
    15421 cgatttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc
    15481 gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag ttggcgttga
    15541 ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg atgtacatcc
    15601 agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg ccctgacggg
    15661 ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc agatgcgctt
    15721 tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag aaagttttat
    15781 gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg ccaacctggg
    15841 cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg acttcacaca
    15901 cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc atgatacagg
    15961 agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg atgttgccgt
    16021 ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa gcggggttag
    16081 ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg cggctttcat
    16141 gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag ttcggctgca
    16201 tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc gcaatcatct
    16261 gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca aattccgtcc
    16321 aagtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa cccagcggga
    16381 tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag cgcatcattt
    16441 cggtttcgct gtgatagcga ttaaacaccg gatgggtgag gatttcgtcg tcgcgcagca
    16501 tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg tcgatctcca
    16561 gcccgtggtt atcgcccagc agcacactga aaagctgcat tacgttttca cgcgtggtgg
    16621 tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg atttcagccg
    16681 cttcggcacg cgtcagtacg cccgctttgt cggctacttc cacacacaag gtgtcgaaat
    16741 agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga atatcggtca
    16801 gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa acggcataca
    16861 ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc gctttctcac
    16921 gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg ccagctgcat
    16981 ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct ttcgccgcaa
    17041 agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc gccgaaccaa
    17101 aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc ataatatcgg
    17161 cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca gtgtagtcgt
    17221 gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc tgatggtcga
    17281 gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca cgagtacgga
    17341 ccacatccag tgtttgcgga tgcacattgg aagccacgaa gaagcggttg gcatttttca
    17401 gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc tcgtccagaa
    17461 gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg aagttgagca
    17521 gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg gtataccagc
    17581 ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg gtgtaaccca
    17641 tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt tctgccagtg
    17701 cggcgtattc ggtcgccggt gcgccaacct gcggtggtgt cgcaagctga atatctttcg
    17761 gcacaatctg gccggtcagc gcgtttaacg attgtgcacc aacggcattc agcatttctt
    17821 gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg ttttcaagct
    17881 ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt gtgaacctct
    17941 ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc ctctcccgca
    18001 gaagaggaat aaagccgtta ctcatcttct aacaatgctt cgtatgcggt cgcatccagc
    18061 agtgattcca gttcgctttc atcgctagct ttgattttaa agatccagcc gcctgcatac
    18121 ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc cacgatttca
    18181 ccgcttactg gcgcataaat gtctgacgcc gcttttaccg attcggcaac cgcgcagtca
    18241 tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat atcgcctaac
    18301 agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc tttacgcagc
    18361 cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa tcaatctcca
    18421 aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt gtcactttaa
    18481 ccggcatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct tccggcacgc
    18541 gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg ataatgcctt
    18601 catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc agcacgcctt
    18661 tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc acttccaggg
    18721 cttcacgacc gataaagtca cgatctgtcg gttcccaggc gatagtccag cccatgttgg
    18781 cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg cccgcttcca
    18841 gacgcagcgt gtcacgcgcc cccagaccgc agggcttaac gcccgcttcc accagcgcac
    18901 gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct tcaccggtat
    18961 aaccagtggt ggcaataaac agatcgccca cctgcacgcc aaagaacggt ttcatccctt
    19021 ccaccgcctg acgctgagca tcattaaaca gtgtggcagc ttttgcctga gcattcggcc
    19081 cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg aaaggttcag
    19141 cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca atgaggcgga
    19201 agaaatcttc agtaaagtag tagacgatga gatcatctat cacaccgcca gaggcattca
    19261 acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg ttcgccagca
    19321 gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc atatgcgaca
    19381 catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat
    19441 gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt
    19501 cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc tgacaacttt
    19561 ttcatggtga aattatcacc acaaaaccca gcatcggagc cactcccggt ccccaacgca
    19621 atcgttctct tttgcctgaa cttaccaccg aaacagactg ttaaccataa ggtaaaattg
    19681 atcatcacat tagcttatgg ttaaaaaatg caaaaatcgc gacagaataa aaaaccaaaa
    19741 aatacaccaa tttctataca aagatgatgt gatgagaaag tcaatttgaa taagacaata
    19801 ttaagagcta aaaaaatgtc aaaaaacact aaatcaaaaa atcatggcat tagaaaatat
    19861 aatgcgaaaa cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa
    19921 accgggagat gaaagtgtga tgggtatcaa ataaacaaca gaggagaaat ttttaacgca
    19981 gccattcaga caaatcgttt aatcccattg cctggcggat aagttgcggc ttaacgccag
    20041 gaagcgtgtc ggccagtttc aaaccaatat cacgcagcag ttttttcgcc ggattagcgc
    20101 cggaaaagag atcgcgaaaa ccctgcatac cagccagcat cagcgccgcg ctgtgcttgc
    20161 ggctacgttc atagcggcgc aggtaaatgt actgcccgat gtctttaccc tgacgatgca
    20221 accgtttcag ttcagcaatc agctctgcgg cgtccataaa tccgagatta acgccctgcc
    20281 ccgccagtgg gtgaatagta tgcgcggcgt cgcccaccag cgccagacgg tgtgcggcaa
    20341 actggcgcgc ataacgcccc gtcagcggga acacctgacg cgcgctctca accttgcata
    20401 agccaaggcg attatcaaaa gcgatattta acgcgcgatt aaattcgtct tcacttgcct
    20461 gctgcatccg ctgcgcttcc tctggcgaca gtgaccagac aatcgagcaa agatgcggat
    20521 cgctaagcgg taaaaaggcc agaatgcctt cgccatggaa aacctgccgc gccaccgcat
    20581 catgcggttc ttccgtgcga atggtcgcta ccagcgcgtg atgctgataa tcccagaaag
    20641 tcagcggaat atctgctttg ttgcgcaacc aggaattagc accgtccgcg ccaatcacca
    20701 gacgcgccgt taacatgctg ccatctttca gcgtcaggaa ggtttcattt tctccccagg
    20761 cgacctgctg taattctgtg ggggccaaca gagtaatatc tgacgactgc tgcgctttgt
    20821 tccacagcgc gtagtgaatc actgagtttt caacgatgtg cccaagatgg ctatagccca
    20881 tgctttgatc gtcaaacgag atgtgaccaa aactgtcttt gtcccacact tccatgccgt
    20941 gatagcaact ggccctacga gcgagaatgt cctgccagac gccaagacgg gtgagtaatt
    21001 tttcgctggc ggcattgata gccgaaacgc gcagttgtgg tagtgcatct accgccagag
    21061 gttccggtac gcgctgctcc agtacggcaa cgcgtaagcc gctcccctgt aagccgcagg
    21121 caaccgccag ccccaccatg ccgccgccaa caatggctac atcaacactt tgcattgttt
    21181 attccttaaa accgccttca acgcgccacc caaccgaggg tgcgctgcgc cagcacatcg
    21241 cgtgccgggg tgaataattc catcgtcatc agtccgatgt tgcgcccgac aaccagcggt
    21301 gcccaacggt tggcaaaaag atgtaccagg ctgtccgtga cgccgatggt tgcttcgcga
    21361 tcgctctgtc gacgctgctg atagcggcac aatatgccgt aatcaccgat gtcttctccg
    21421 cgctcatgcg cctgagtcag ggtttccgca aggctcatga catctcgcat accgaggtta
    21481 aacccttgcc cggcaatcgg gtgcagagtt tgcgccgcat tgcccaccag cacagtgcga
    21541 tgggtaatcg gtctggcggc gcgggttaat gccagcggat aagcactgcg tttaccggcg
    21601 tgggtaattt gccccagtcg ccagccaaag gcagactgga gttcacggca aaacttctcg
    21661 tcgctccacg acagcacctc ttcgcgccgt tccagtggat gacaccagac cagcgaacag
    21721 cgtccgtcag acatcggcaa catcgccagc gggccatgtt gcgtgaagcg ttcaaaagcg
    21781 cggccttcgt gcgcaacaga agtagcaacg ttggcaatca ctgccagttg ttcgtaaggc
    21841 tcctgctgcc aatcaacgcc gcacgcggtg gctaacgctg aatgggtgcc atcagcggct
    21901 atcagcacgc ggcccgtcag catctcgccg ctctccagcg tcacttcaac gtgactctga
    21961 gtacgggcaa cgttagctac gcgatcgggg caatgcagca ttacgccagg tgcttttcgc
    22021 agcaatgcaa acagccgttg cccaacatta tgcaattcga caacctgccc cagcgccgcc
    22081 agttggtaat cttctgcggc gagggtgacg aatccagcat gaccacgatc gctgacatgc
    22141 acggtagtga tggcggttgc gcaatcagcc agagattgcc agacgccgat gcgcgccagt
    22201 tgctgacagg tacccgccgc cagcgctatc gctcgtccat caaagcccgg atgagcatgt
    22261 gactctggcg cagtcgcttc aatcaaatgt accggcagcg ccccgtgact taaccgggaa
    22321 atagccagcg ccagcgtcgc gcccgccatg ccgccaccga cgatgattac gctcattgct
    22381 ttctcgcagc agccatcaac gcttcgattt cttccggctt tttcaccacg ctggcggtga
    22441 ggttttcgtt gccggtttcg gtaatcacaa tgtcgtcttc aatacgaatg ccgataccgc
    22501 gatattgttc tggcacatcc gcatccggcg cgatatacag accaggctct acggtcagta
    22561 ccatccccgg ttccagaatg cgcgagcgat cctgaccata aacgccaacg tcatggacat
    22621 ccagtcctaa ccagtggcta aggccatgca taaagaaagg acgatgggcg ttctgagcga
    22681 tcagttcatc aacattacct ttcaggatgc cgagttttac caggccgcta accatgatgc
    22741 gcaccacttc accagtgact tccagaatgg aagttcccgg acgatacagg cgcaggctgg
    22801 tttcgagaga ctccagcaca atgtcgtaga tttcacgctg ggcctgggtg aatttgccgt
    22861 tgaccgggaa ggtgcgggta atatcgcccg cgtaaccttt gtattcacaa cccgcgtcaa
    22921 tcaacaccag gtcaccgtcg cgcagttcac tctcgttttc ggtgtagtgc agaatgcagc
    22981 cgttttcacc gctgcctaca atggtgttat aggaaggata gcgcgcaccg tggcggttaa
    23041 attcgtggtg aatttcgcct tccagatggt actcgaacat tcccggacgg catttttcca
    23101 tcgcccgtgt atgtgccatg gcggtgattt ctcccgcgcg gcggagtacg gcaatctctt
    23161 ctggcgattt gaacaggcgc atttcatgaa caacaggacg ccagtcgatc atcgttgccg
    23221 gtgcggtgag attttgccgc gaacctttac gcagtttttc cagcgcactg ttcacgattt
    23281 catcagcata tgcatattcg ccctgggcat ggtaaaccac atccaggccg ttaagtagtt
    23341 gataaagttg ctgattgatt tcgctgaatg ccagtgcgcg gtcaacgccc agtttctctg
    23401 gcgcggcatc ctggcctaag cgacggccaa accagatctc cgccgtcagg tcgcgaacgc
    23461 ggttaaacag aacgctgtgg ttatgagtgt catcgctttt aatcagcacc agcaccgctt
    23521 ccggttcgtt aaagccggtg aagtaccaga agtcactgtt ctgacgatag gggtattcgc
    23581 tgtcggcgct acgtgttact tctggtgcag caaaaatcag cgcggcgctg ccgggttgca
    23641 tttgctccac cagggcctga cggcgacgct gaaactcttg ccgggatatc tcactcataa
    23701 cactctcctt acgttttttg tttttagtgt agagtcggtt tttgtacttc tggcgcggtc
    23761 ggttgcggat gagtaaaggt gtcgtggcat aacagcgcgg caacacgaac gtattcgatg
    23821 atctcttcaa gcgacatttc aagctcttcc tgatcttcgt cttcgtcgta gcccagctgc
    23881 gcgatgttac gcagatcgtc gatggcttca ccggtttcgc cggtcacttt gtccagcttc
    23941 ggttgcgtaa cgccaagacc aagcaggaag tgattgaccc aacctgccag tgcatcagcc
    24001 cgatcgaaaa cgctgacatc atcgccatca ggcagataaa gctgaaaaag gaagccgtca
    24061 tcctgcaggg catcgctggt ggcagagtgc attttacgca gtgcctgtgc cagctcatga
    24121 ccgaaagcca tgccttcgtt cgtcaggtcg tgaagtagcg gcagccatga gctgtcatcg
    24181 ttaccgccac atatcatccc actgattaaa ccatgcatct cggcaggggt cagacccgtc
    24241 ccttgttggt tcagatactg gttcatttcg ttgtaaccag gcatttcgtt ctgtatagac
    24301 ataagcattc gtcgtcaaag ggaggaatat tcatgatatg ctaccacttt gggccctggt
    24361 ggaccagaaa agggcttgtc tcttctcatc agggtagcta tagcgtcgcc ccttcgcaga
    24421 ccatgggtct aaagacgaag gcagcgcagt caatcagcag gaaggtggca tgtctgcaca
    24481 acccgtcgat atccaaattt ttggccgttc actgcgtgtg aactgcccgc ctgaccaaag
    24541 ggatgcgttg aatcaggcag cggacgatct gaaccaacgg ttgcaagatc tgaaagaacg
    24601 cactagagtc acaaatactg aacagttggt cttcattgcc gcattgaata tcagctatga
    24661 gttagcgcaa gaaaaagcaa agactcgtga ctacgcggca agtatggaac agcgtattcg
    24721 gatgctgcag cagaccatag aacaagcgtt acttgaacaa ggtcgcatca ccgaaaaaac
    24781 taaccaaaac tttgaatgac acttttcggt ttactgtggt agagtaaccg tgaagacaaa
    24841 atttctctga gatgttcgca agcgggccag tcccctgagc cgatatttca taccacaaga
    24901 atgtggcgct ccgcggttgg tgagcatgct cggttcgtcc gagaagcctt aaaactgcga
    24961 cgacacattc accttgaacc aagggttcaa gggttacagc ctgcggcggc atctcggaga
    25021 ttcccttctt atctggcacc agccatgacg caactaccag agctcccact gacattatcc
    25081 cgacaagaaa tccgcaaaat gattcggcaa cgtcgtcgtg cgttaacgcc ggaacaacag
    25141 ctggaaatgg gtcaacaagc cgctacccgg atgatgactt atcccccggt ggtgatggcg
    25201 catacggtcg ctgtattcct ctcttttgat ggtgaactcg acacccagcc gctaatagaa
    25261 caactctggc gcgccggtaa gcgcgtatat cttccagttt tgcatccctt tagtgccggt
    25321 aatttgctgt tcctgaatta ccatccgcaa agcgaactgg tgatgaacag gttgaagatc
    25381 catgagccaa aactggatgt gcgtgacgtg ctaccccttt cccgattaga cgtgctgatc
    25441 acaccgctgg tcgcctttga tgagtacggt cagcgcctgg gaatgggcgg tggtttttat
    25501 gatcggacct tacaaaactg gcagcactat aaaacgcaac cggtgggtta tgcgcatgat
    25561 tgtcagttgg tggaaaaact ccccgttgaa gagtgggata tccctcttcc tgcggtggtt
    25621 acaccgtcga aagtctggga gtggtaaggg cgatacacct gcatcgccct gattgacatc
    25681 gttgattctt taacctaatt tagtgagtaa gggtaaggga ggattgctcc tcccctgtga
    25741 ctgttaataa gctgggaaac ttatgagtaa cagtacaatc agtatgatgg caagtcgcat
    25801 cataaccctt ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc
    25861 tgcccagcta tcgtaagctc tctttaaata accacaacgc attgatctac ctttaaatct
    25921 tttcttcgag cgtgctcgaa aaaccccatg taaaacagag ataatttttg ggaaaaagga
    25981 tctattacgg cagtaaacgt gggcgatgaa gtggctatca aaaagttcgg ttcagggcga
    26041 tgcaccccgc atcgccctga ttgacatcgt tgattctttg acctaattta gtgagtaagg
    26101 gtaagggagg atgactcctc ccttgagact gactgttaat aagctccgaa acttatcagt
    26161 aacagtacaa tcagtatgat gacaagtcgc atcataaccc ttctccttta agccctcgct
    26221 tcggtgaggg ctttaccgtt acagccccat gctgtcccgc catcgtaaat ccccattaaa
    26281 taaacacaac gcattgatct gactttgatt tattttctgg agcagactcg caaagtagaa
    26341 tgcgcaacgc ggcaacggtg tggagaaggg ataaaaaaac gggcaagtca gtgacctgcc
    26401 cgttaatttt cagagaaggg gaattagtac agcagacggg cgcgaatggt acccggaata
    26461 gctttcattg cctgcagcgc tttttcggca acgtcttcgt cagcttcaat atcaataact
    26521 acataaccca tctgggcgga agtttgtaga tattgcgcgg cgatgttgac gccctgctcg
    26581 gcgaagattt tgttcagcgc agttagcacg cccggacggt tttcgtggat gtgcatcaga
    26641 cgacgaccac catgcagtgg cagcgagact tccgggaagt tcaccgcaga gagcgttgag
    26701 ccattgtcag aatacttgat caatttaccc gcaacttcca agccgatatt ctcctgcgct
    26761 tcctgagtcg aaccgccaat gtgtggcgtc agaaggacgt tgtcgaattc acacagtgga
    26821 gaggtaaatg gatcgctatt ggtcgccggt tccgtcggga atacgtcgat tgccgccccc
    26881 gccagatgtt tgctcgccag cgcatcacac agcgccggaa tatccaccac agtaccgcgc
    26941 gaggcattaa tcagcagcga gccgggcttc attagcgaaa tctctttcgc gcccatcata
    27001 tttttggtgg acggattctc tggtacatgc agactcacca catcgctcat attcagcagg
    27061 tcagaaagat gctgtacctg agtggcgttg cccagcggca gtttgttttc aatatcataa
    27121 aagtaaacat acattcccag cgattcagcc agaatgccca attgcgtacc aatatgaccg
    27181 tagccgatga tacccagctt tttgccgcgc gcttcaaaag aacccgccgc cagtttgttc
    27241 cacacgccac ggtgcgcttt agcattggct tccggcacgc cgcgcaatag cagcagcagt
    27301 tcgccaatca ccagctccgc aacagagcgc gtatttgaga acggtgcgtt aaataccggg
    27361 atcccgcgct ttgccgccgc atccagatca acctggtttg ttccgataca gaaacagcca
    27421 atagcgacca gtttttctgc ggcgttgatc acgtcttcag tcagatgggt acgggatcgc
    27481 aggccgatga agtgggcatc gcggatggat tcttttaatt gttcatcatc cagcgcgcct
    27541 ttgtgaaatt cgatgttggt gtaaccagct gcacgaaggc tttccagcgc cttttggtgc
    27601 acgccttcta ccagcagaaa cttaatcttg tctttctcca gcgatacctt tgccatttac
    27661 ccaatcctgt cttttgaaat gttgtgtgcg gatttgcatc cgcctttcaa catatcaaaa
    27721 aataatattg cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat cacgcaaagg
    27781 taattcttgg atgaatatgc tggtttagag gatttattta gagcaatcga caattgcctg
    27841 gtaaaagcgt gacacatgtc accaaattta atgaagagaa tttttttaac gggggaggtt
    27901 cccccgtcag atcatttcac aatggttttg acaccgtcag gtgtgccaat cagcgcaacg
    27961 tccgcgccac ggttagcaaa caagccaaca gtcaccacgc caggaatcgc atttatggcg
    28021 ttttccatcg ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat cacgttgcca
    28081 ttatcggtca ccacgccctg acggtattcc ggacgaccgc ccagtttcac cagctggcgc
    28141 gccactgcac tacgtgccat cgggataact tctactggca gcgggaattt acccagaata
    28201 tcaacctgct tggaggcgtc ggcaatacag ataaattttt ccgcaaccga agcaatgatt
    28261 ttttcacggg tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc gttgatttca
    28321 tctgcgccat caacgtagat gccaaggctg tcgacttcgt tgagatcaaa aacgtgaatg
    28381 ccgaggcttt tcagtttttc agtggaagca tctgaactgg aaacggcccc ttcaatctgg
    28441 cctttcatcg tgccgagcgc gtcaataaag tgtgcggcgg tggaacctgt acctacacca
    28501 acaatggtac cgggctgaac atactgaagt gccgcccatc ctactgcttt tttcaattca
    28561 tcctgcgtca tgatcgtttc gcctgtggta tgaaatttca cacgcattat atacaaaaaa
    28621 agcgattcag accccgttgg caagccgggt ggttaactca tccataaaat atcgcgcaat
    28681 ggcgggcatc ccctttcgcc ccgcaaataa agcatacaac ggtctgggta tgccgctcca
    28741 cggtgcgaac agaggcaata acgccacgcc attcaacata accagggcgc gaagtcgcta
    28801 agcgaaatgc aatagcgcaa atccttgatc ttttaagctg cgcgtttaac cattgataca
    28861 aatgttaccc gccgacggct tcggtatatg caacctgaca caaaattgtg tcatagtgca
    28921 ggaaaaagca tttaccagga gcagacaaca gcaatgaaac gcccggacta cagaacatta
    28981 caggcactgg atgcggtgat acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc
    29041 attacacaat cagccgtctc acagcgcatt aagcaactgg aaaatatgtt cgggcagccg
    29101 ctgttggtgc gtaccgtacc gccgcgcccg acggaacaag ggcaaaaact gctggcactg
    29161 ctgcgccagg tggagttgct ggaagaagag tggctgggcg atgaacaaac cggttcgact
    29221 ccgctgctgc tttcactggc ggtcaacgcc gacagtctgg cgacgtggtt gcttccggca
    29281 ctggctcctg tgttggctga ttcgcctatc cgcctcaact tgcaggtaga agatgaaacc
    29341 cgtactcagg aacgtctgcg ccgcggcgaa gtggtcggcg cggtgagtat tcaacatcag
    29401 gcgctgccga gttgtcttgt cgataaactc ggtgcgctcg actatctgtt cgtcagctca
    29461 aaaccgtttg ccgaaaaata ttttcctaac ggcgtaacgc gttcggcatt attgaaagcg
    29521 ccagtggtcg cgtttgacca tcttgacgat atgcaccagg cctttttgca gcaaaacttc
    29581 gatcttcctc ctggcagcgt accctgccat atcgttaatt cttcagaagc gttcgtacaa
    29641 cttgctcgcc agggcaccac ctgctgtatg atcccgcacc tgcaaatcga gaaagagctg
    29701 gccagcggtg aactgattga cttaacgcct gggctatttc aacgacggat gctctactgg
    29761 caccgctttg ctcctgaaag ccgcatgatg cgtaaagtca ctgatgcgtt actcgattat
    29821 ggtcacaaag tccttcgtca ggattaatcc atcaaataat gcctgatagc acatatcagg
    29881 cgttgtcctc acttcttttt gtattccttg aatcacatca caaaatagac aaat
//
LOCUS       DAJTRQ010000036        28370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000036 DAJTRQ010000000
VERSION     DAJTRQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="OO332_001713"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="OO332_001713"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5942223.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="OO332_001714"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="OO332_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5942224.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="OO332_001715"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="OO332_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5942225.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /gene="ghxP"
                     /locus_tag="OO332_001716"
                     /gene_synonym="yjcD"
     CDS             2778..4127
                     /gene="ghxP"
                     /locus_tag="OO332_001716"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5942226.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="OO332_001717"
     CDS             4278..5927
                     /locus_tag="OO332_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5942227.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="OO332_001718"
     CDS             complement(6081..7373)
                     /locus_tag="OO332_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5942228.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="OO332_001719"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="OO332_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5942229.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="OO332_001720"
     CDS             complement(9197..9511)
                     /locus_tag="OO332_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5942230.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="OO332_001721"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="OO332_001721"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR5942231.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="OO332_001722"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="OO332_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5942232.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="OO332_001723"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="OO332_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5942233.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="OO332_001724"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="OO332_001724"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5942234.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="OO332_001725"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="OO332_001725"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5942235.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="OO332_001726"
     CDS             15750..17468
                     /locus_tag="OO332_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5942236.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="OO332_001727"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="OO332_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5942237.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="OO332_001728"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="OO332_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5942238.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="OO332_001729"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="OO332_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5942239.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="OO332_001730"
     CDS             complement(20170..20859)
                     /locus_tag="OO332_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5942240.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="OO332_001731"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="OO332_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="OO332_001732"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="OO332_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5942241.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="OO332_001733"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="OO332_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27750)
                     /locus_tag="OO332_001734"
     CDS             complement(26812..27750)
                     /locus_tag="OO332_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5942242.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27769..28044)
                     /locus_tag="OO332_001735"
     CDS             complement(27769..28044)
                     /locus_tag="OO332_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942243.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28253..>28370)
                     /locus_tag="OO332_001736"
     CDS             complement(28253..>28370)
                     /locus_tag="OO332_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942244.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6472 a         7178 c         7615 g         7105 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg ggggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcag ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tcattttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac aagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagca tgctgtcgtg ggaactcaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctgttgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta gcagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagcagatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttctg cggcgcgctg cgccgttctt gcacggtcaa cgtcttctgc
    27301 tgagacaaaa ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt
    27361 cgcggcggca cgggcttttt ctaccgtggc attaaccgag tcggcaccaa actgttgggc
    27421 gtcaacgcta cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcggc
    27481 tttcgccagg ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt
    27541 caccgcctgg ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac
    27601 atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca
    27661 gataacgaaa acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact
    27721 gcgaggtgct tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc
    27781 cagccagaac agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc
    27841 cagattgata tttgcccgct gaataactcg cctcgtgatg agcgtcaaaa taaggctggc
    27901 aatggcgcag aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa
    27961 tgagcatccc gaaaccatga agacgggaat tatccaggct gtttttttga ggtgtatcgc
    28021 catgcgagag agaactgttg gcatattaaa tacatcctgt ctgctgcgaa caatatctgt
    28081 agtgaatatc aatatccatt gaaggcgtca gtatggctat tttctcgatg aataaaaata
    28141 gctttatcca gccaccgcat tcataactta tatattttat ttattaattg gctgtatata
    28201 tttttaaaat aaatgctttt gggggcttca gcgcccccaa aagcgagtga cattaatttg
    28261 gggttacgat attcacccac aaatcgaaat tatccagaca ggcgataatt tcttccagtt
    28321 tcgcgccatt gccaataatt ttggctctct tcgctttcac cagatccgcc
//
LOCUS       DAJTRQ010000037        28025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000037 DAJTRQ010000000
VERSION     DAJTRQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(98..388)
                     /gene="ubiK"
                     /locus_tag="OO332_001737"
     CDS             complement(98..388)
                     /gene="ubiK"
                     /locus_tag="OO332_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5942245.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     regulatory      510..658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            762..1415
                     /gene="ribB"
                     /locus_tag="OO332_001738"
     CDS             762..1415
                     /gene="ribB"
                     /locus_tag="OO332_001738"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5942246.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1676..1846
                     /locus_tag="OO332_001739"
     CDS             1676..1846
                     /locus_tag="OO332_001739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942247.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="OO332_001740"
     CDS             complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="OO332_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5942248.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2820..3608
                     /gene="ygiD"
                     /locus_tag="OO332_001741"
     CDS             2820..3608
                     /gene="ygiD"
                     /locus_tag="OO332_001741"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5942249.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3646..4806)
                     /locus_tag="OO332_001742"
     CDS             complement(3646..4806)
                     /locus_tag="OO332_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5942250.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4812..5483)
                     /locus_tag="OO332_001743"
     CDS             complement(4812..5483)
                     /locus_tag="OO332_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5942251.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="OO332_001744"
     CDS             complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="OO332_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5942252.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7317..7946
                     /gene="nudF"
                     /locus_tag="OO332_001745"
     CDS             7317..7946
                     /gene="nudF"
                     /locus_tag="OO332_001745"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5942253.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7947..8369
                     /locus_tag="OO332_001746"
                     /pseudo
     CDS             7947..8369
                     /locus_tag="OO332_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8394..9221
                     /gene="cpdA"
                     /locus_tag="OO332_001747"
     CDS             8394..9221
                     /gene="cpdA"
                     /locus_tag="OO332_001747"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5942254.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9221..9802
                     /gene="yqiA"
                     /locus_tag="OO332_001748"
     CDS             9221..9802
                     /gene="yqiA"
                     /locus_tag="OO332_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5942255.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9831..11723
                     /gene="parE"
                     /locus_tag="OO332_001749"
     CDS             9831..11723
                     /gene="parE"
                     /locus_tag="OO332_001749"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5942256.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11771..12085)
                     /locus_tag="OO332_001750"
     CDS             complement(11771..12085)
                     /locus_tag="OO332_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5942257.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="OO332_001751"
     CDS             complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="OO332_001751"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5942258.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13016..13348
                     /locus_tag="OO332_001752"
     CDS             13016..13348
                     /locus_tag="OO332_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5942259.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="OO332_001753"
     CDS             complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="OO332_001753"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5942260.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="OO332_001754"
     CDS             complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="OO332_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5942261.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15551..15943
                     /gene="ygiW"
                     /locus_tag="OO332_001755"
     CDS             15551..15943
                     /gene="ygiW"
                     /locus_tag="OO332_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5942262.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15996..16478
                     /locus_tag="OO332_001756"
     CDS             15996..16478
                     /locus_tag="OO332_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5942263.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17024..19282
                     /gene="parC"
                     /locus_tag="OO332_001757"
     CDS             17024..19282
                     /gene="parC"
                     /locus_tag="OO332_001757"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5942264.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19516..20253
                     /gene="plsC"
                     /locus_tag="OO332_001758"
                     /gene_synonym="parF"
     CDS             19516..20253
                     /gene="plsC"
                     /locus_tag="OO332_001758"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5942265.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20328..21740
                     /gene="ftsP"
                     /locus_tag="OO332_001759"
     CDS             20328..21740
                     /gene="ftsP"
                     /locus_tag="OO332_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5942266.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21851..24068
                     /locus_tag="OO332_001760"
                     /pseudo
     CDS             21851..24068
                     /locus_tag="OO332_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="OO332_001761"
     CDS             complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="OO332_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5942267.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24419..25345)
                     /locus_tag="OO332_001762"
     CDS             complement(24419..25345)
                     /locus_tag="OO332_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5942268.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="OO332_001763"
     CDS             complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="OO332_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5942269.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="OO332_001764"
     CDS             complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="OO332_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5942270.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27834..>28025
                     /locus_tag="OO332_001765"
     CDS             27834..>28025
                     /locus_tag="OO332_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5942271.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6567 a         6946 c         7443 g         7069 t
ORIGIN      
        1 gtgatgatac tctgaatggg attaagcgat aatgtaatag aaaacactgt aaattagagg
       61 attaattcat aatttacagt gcattaaaga caaatgttta cagcgttggg gggagagtct
      121 ctggatctgg ctgctttttg atctcagtac tgcgattttc cagttcgctg attctttgtt
      181 caagcagcgc cagtttctca cgagtccgta acaggacttg cgtttggacg tcgaactctt
      241 cacggcttac aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg atttttttct
      301 ccacatcttc cccgaactcc ctgattcctt taggcattga ttcgtgaacc tggcgagcga
      361 tttgctcaat ttttttcggg tcaatcattg tagtttccct gtactgatag gtgttgagta
      421 ccattgtagt gcgataaggg taagtcataa accagaatta tgtgaagcta tgcgttgctg
      481 ccgctaatca ttagagttat agtgaatccg cttattctca gggcggggcg aaattcccca
      541 ccggcggtaa atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa ctcaaaggac
      601 agcagatccg gtgtaattcc ggggccgacg gttagagtcc ggatgggaga gagtaacgat
      661 tctgtcgggc atggacccgc tcacgttatt ttggctatat gccgccactc ctaagactgc
      721 cctgattctg gtaaccataa ttttagtgag gtttttttac catgaatcag acgctacttt
      781 cctcttttgg tacgcctttc gaacgtgttg aaaatgcact ggctgcgctg cgtgaaggac
      841 gcggtgtaat ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg atcttcccgg
      901 cagaaaccat gaccgttgag cagatggcgc tgaccattcg ccacggtagc ggtattgttt
      961 gcctgtgcat cactgaagat cgccgtaaac aacttgatct gccaatgatg gtagaaaata
     1021 acaccagcgc ctatggcacc ggttttaccg tgaccattga agcggctgaa ggtgtgacta
     1081 ccggtgtttc tgccgctgat cgtattacga ccgttcgcgc agcgattgcc gatggcgcaa
     1141 aaccttctga tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag gcaggtggtg
     1201 tactgacgcg tggcggtcat actgaagcga ctattgattt gatgacgctg gcaggcttta
     1261 aaccggctgg tgtgctgtgt gagctgacta atgacgatgg cacgatggcg cgtgcaccag
     1321 agtgcattga gtttgccaat aaacacaata tggcgctcgt gactattgaa gacctggtgg
     1381 cataccgtca ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat tactgcctta
     1441 atcaagaaac cgaagttgta gctggcttcg gtttttattt tgccctgcta tgcaagatgt
     1501 taattccgct tctctttcta tgagaaaatt tcattaatat caggcattct ttttcattat
     1561 gatcaaaatc atataattgt tccgttatta tgcgaccata tgaatatatc cgaaaaagga
     1621 tatgtgaaat ttatgcgcta aatgtttagt attgttaaat attaagggta aaaggatgtt
     1681 tatcgcctgg tactggattg tattgattgc tctggttgtg gtgggttatt tcctgcattt
     1741 gaaacgttat tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag cgcggaacaa
     1801 atacttaaac agtacgagag aagagacagc cgaaaaggta gaataaggct ttcgtgaagc
     1861 tggcagaagt tgtccatgtg ctggacaact tctgatgttg tttttaacca attcccgccg
     1921 tttgtagcag cactaaactg aatcccatca ctgacattcc acatagtacg ccatagctgg
     1981 ggttattatt agggtcaatc tctttggcga gcggcattaa ttcatcaacc gagagcgcca
     2041 ccataattcc tgcaaccgcc gccatgattg ccgccatgac caccggggaa atcatgctac
     2101 cgaggattaa ccacgccagc acaccaccaa gaatttctgc tagtccagaa atccccgccc
     2161 acagaatcgc ggtgcgttta gaccccgttg ccgcataaac cgggcctgcc actgccagac
     2221 cttcagggat attgtgcaac gcgacggcca gtgcgatgcc aaatcccagc tccaggttgc
     2281 tgctcgccgt gacaaaggtg gcaatccctt ccgggaagtt atgcagactg atgccgagag
     2341 tgagcagaat ggctgtgcgc ttgatcgatt ttggcaacag ctgcaccgat ttttgcatta
     2401 aatcctgcgg atgcgcatgc ggcaacatgc ggtccaggcc aaagtagcca agcagaccga
     2461 agataaacat tccataaccc aacaccggcg acattccttc agctgctagt gcggcaggaa
     2521 gcatttccat taatgagatg agcaacatga tccccgccgc aaaacctagc gaaaacgcca
     2581 gtaagcggtt cgagggtttt tgtccgagaa cgccgagaaa cgcgccaata aacgtggctg
     2641 cccccgccag tatggtcaga atgagaggta ctgacatcga caactcctta taaatcttca
     2701 ccttcaggca gatcgtcaaa ataactgatg atcatcatca ttgttatccg ggttcttact
     2761 ccgccgaaca agcgtattgt gaggatatca aaatgacacc tttagttaag gatatcatca
     2821 tgtcttcaac acgtatgcca gcattgtttt taggtcacgg tagtccgatg aacgtgctgg
     2881 aagataattt gtatacccgc agctggcaga agttggggat gacattgcca cgcccgcaag
     2941 cgattgtggt ggtttcggct cactggttta cccgtggaac aggagtgacc gcgatggaga
     3001 cgccgcccac gattcatgac tttggtggct tcccgcaggc gctgtacgat acgcattatc
     3061 ctgctccggg ttcgcctgcg ctggcacagc gtctggttga gctgttagcg ccgatcccgg
     3121 tgacgctgga taaagaagcc tggggctttg accacggctc gtggggcgtg ctgattaaga
     3181 tgtatcctga cgccgatatc cctatggtgc agttgagtat cgacagtagc aaacctgccg
     3241 cctggcattt cgaaatgggg cgcaaactcg cggcgttgcg tgatgaagga ataatgttgg
     3301 tcgccagcgg taacgtggtg cataacctgc gcacagtgaa gtggcacggt gatagttcac
     3361 cgtatccgtg ggcgacgtcg tttaatgagt atgtgaaagc gaatctgacg tggcaagggc
     3421 cggtagagca gcatcctctg gtgaattatc tcgaccatga aggtggcgcg ttatcgaacc
     3481 caacgccaga gcactatctg ccgttgttgt atgtgttagg tgtgtgggat gggcaggagc
     3541 caattaccat tccggtcgat ggtatagaaa tgggcagttt aagtatgctg tcggtgcaga
     3601 taggctaaca attaccggat ggcgagtgcc atccggtatc gtggcttatt caacaaaaat
     3661 atgtggataa aaccgagaca tatcctgggt gatcaacgca cggtcttcac gaatgccaat
     3721 tccggcaggt tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt
     3781 cggtaacggg tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc
     3841 ttcaatggtt ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg
     3901 tttaaccaca tatttttcca tttgcggatg atcatcttcc gcaaaataag cgggcagcag
     3961 gttcgggtga ttcgggaaca tctcccacag tagcggtaga agtgccttgt tggagataat
     4021 actcttccac gccggttcca gccagcgtac gccagcatct tccagcttgg tggagaacat
     4081 ttcgcgcagc ataaattccc acggataaag cttgaacagg ttagaaatca cctgatcctg
     4141 taaatccgtg aactggcctt tttcgcctaa acctatctcg tcgatgtaga ggaactcggt
     4201 ggcgatttca gcttcggttg cgcagtcctg caaatactga atggttccgc gatcttccac
     4261 cgtgtcgcga cagcaggtga gatgcagcaa ctggaagcca cactgttcac gcagctcaac
     4321 gaagcgatcg atcagttttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt
     4381 accggcgtta agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga
     4441 cgttggcgta tcggcgttat tttccagaag tttaggttcg ccagtgccat cccacgccag
     4501 atcaagacgc gaataaagcg atggctggtg agtcagccat gattgacgca caaaactcca
     4561 ggtgtgtttt ggtatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac
     4621 cactttcagg cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc
     4681 gagggtcaac ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa
     4741 attgaaaccg tattcgtggg ctttctcacg ccagtccggg cgctcggtaa tactgactct
     4801 ttccatcggt atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata
     4861 gtgctttgtt tggcaacaga ttcaccaaaa ccgccacggg taacggtagt ggtggtcgcc
     4921 ggttttggtg ccattgccgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca
     4981 tagtttttac ccgtcgcgtc ggtatattta ccgtaagccg gactggccgg gtttttcgag
     5041 gagaacagcg gctgctgtgc aaatcccgcg ccgccgccca tcagacgccc catcatgtaa
     5101 ccggccatca gcggcatcca gaaactcccg ctggattgct gagcctgcgc ctggttttct
     5161 ggtgccatgc cagcctgggc tggtgcttgc tgacactgac cttcaccaaa ttcagcaaca
     5221 cagtcttcac gggtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg
     5281 tacgcggtgg tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt
     5341 tgatagagag acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca
     5401 accgcgagag cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg
     5461 cgtatggatt ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga
     5521 atagaggatg gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt
     5581 atctttacgt tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtca tcagttacgg
     5641 aaagggttat gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gacgggtgcc
     5701 gggctatcag gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggggcaacg
     5761 ttttccggat tagtggaaac cggtttgctc agcgcattgt tcagtgccag caaatcctgc
     5821 tcgttcaacg tacccagggc tgacttaata ttcagctgat taatcaggta gttataacgc
     5881 gcattcgcca gctcttgctt ggcgttgtac agcgtggtgg tcgcatccaa cacatcaaca
     5941 atggtacgcg taccgaccga gtagcccgct tccatcgcgt ctaatgagct ttgcgcggaa
     6001 actacggctt gtttgtaggc gttaatacta ctgatagaag cattaatgtt gttgaaggag
     6061 gaacgtacgg tttgcacgac gctacgatgt gcgctttcca gttgctcact ggcaccaaca
     6121 aagttgtact gtgcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc
     6181 gagaagctca ggccaacttt gttctggccc atattgctat cgtcatactg ggtaccagcg
     6241 gcaccacggg ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa
     6301 tccagagtcg gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg
     6361 ctcaagcatg cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgcgtta
     6421 accggctgtg gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttccggatag
     6481 tagttaccgg tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc
     6541 acttcgttcg ccagcacggt atcgtactgc gcgcgggcgt tctgcacgtc ggtgatcgct
     6601 accaggccca cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt
     6661 tgtgcctgtg tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg
     6721 gtgttgagga tcaaggtttg ctgatcggtc tgatatgtga cgtcctgaat ccctgctgct
     6781 ttttcctgca gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc
     6841 agggacgcac tggtcgcgtt cgagttgatg ccgttcgcgt cgcggtagcc gttgctatag
     6901 gtgtaatctg cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt
     6961 tcaaaggcag catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct
     7021 tgctgataaa cttgcatcag gttctcggcc tggctcaacg aactgaaccc agaaaggctc
     7081 aggccgataa gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt
     7141 agcgcgatca aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt
     7201 ggcgttacgt gccatagggc gttaatgtgc ctcaatttaa catatgactg ctcaaacggc
     7261 agtcaaacgt cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc
     7321 ttaagccaga caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag
     7381 aaacacttta tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca
     7441 acgggcaaat gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct
     7501 tgctaccctt tgacccagtg cgtgatgaag ttgtgctgat tgagcagatt cggattgccg
     7561 cgtacgacac cagcgaaacc ccctggctac tggagatggt tgccgggatg attgaagagg
     7621 gtgaaagtgt ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca
     7681 aacggaccaa accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt
     7741 caattatggt gggcgaagtg gacgccacga ccgcaagcgg tattcatggt ctggctgatg
     7801 aaaacgaaga tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag
     7861 aggggaaaat cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc
     7921 aagcgttaaa aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga
     7981 tgcgcctgtg cgagatgaac ttttcataat tgcgccgttt gttaccgcgc aattacgcac
     8041 ccggcgaaac tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat
     8101 cgacccgata cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga
     8161 gccttccgtc aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa
     8221 gccagcagat ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac
     8281 gcgacgaaaa gcatcaaatt aaccagtttt tagcggactg gttgcgatac tgtttagcac
     8341 atggagcgat ggcgattccg gtttactagc gtcgtgaaac ctaaggacac catttggaaa
     8401 gcctgttaac ccttcctctg gctggtgagg ccagaggaag gattttacaa attaccgata
     8461 ctcacctgtt tgcacaaaag cacgaagccc tgttaggggt aaacacctgg gaaagctacc
     8521 aggcggtgct ggaggcgatt cgtccacacc agcacgaatt cgacctgatt gtcgcgacag
     8581 gtgatttagc gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa
     8641 gttttcgtgc gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca
     8701 gcgcgttaca ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc
     8761 aaatcctgtt gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc
     8821 agcttgagtg gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc
     8881 tgcatcatca tccgctacct gcgggttgta gttggctcga tcaacacagt ctgcgtaacg
     8941 cgggcgaact ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc
     9001 atattcatca ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga
     9061 cctgtgtgca gtttaagccg cactgttcca actttacgct ggatactatc gcgcccggct
     9121 ggcgtactct cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctagcgg
     9181 acacacgttt ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt
     9241 acacggtttc aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggctggc
     9301 ggaacatcac cctgacgttg agatgatcat tccgcagttg ccgccgtatc cttccgacgc
     9361 ggcagaactg ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg
     9421 ttcgtcactg gggggatatt acgccacctg gttgtcgcaa tgttttatgc tgcccgcagt
     9481 ggtggtaaac ccggcggtac gcccgtttga attgctgacg gactatctcg gtcagaacga
     9541 gaacccctac accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt
     9601 catgcagatt gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga
     9661 tgaagtgctg gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat
     9721 agaaggcggc aaccacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt
     9781 tcttggtctg caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa
     9841 cttataacgc tgatgccatt gaggtactca ccgggcttga gccggttcgc cgccgtccgg
     9901 ggatgtatac cgataccact cgccctaacc atttggggca agaagttatt gataacagtg
     9961 tcgatgaagc actggcgggt cacgcaaaac gcgtggatgt aatcttacat gctgaccagt
    10021 cgttagaagt gattgacgat gggcgcggga tgccggtgga tattcacccg gaagaaggag
    10081 taccggcggt tgaactgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa
    10141 attaccagtt ctctggcggc ctgcatggcg tggggatttc ggtagttaac gccttgtcga
    10201 agcgcgtaga agttaacgtg cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg
    10261 gcgaaaaggt gcaggattta caggttgtcg gcacttgcgg taaacgcaat accggtacca
    10321 gcgtgcactt ctggccggat gaaaccttct ttgacagccc gcgtttttct gtttcacgcc
    10381 tgacgcatgt gctgaaggcc aaagcagtac tgtgccccgg cgttgagatt acctttaaag
    10441 atgagatcaa caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg
    10501 cggaagcggt aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg
    10561 gcgatactga agctgtggac tgggcgctac tgtggctgcc ggaaggcggt gaactgctga
    10621 ccgaaagcta cgtcaacctt atcccaacga tgcagggcgg tacccatgtt aatggtctgc
    10681 gtcagggcct gttggacgcg atgcgtgagt tctgtgaata ccgcaacatt ctgccgcgcg
    10741 gcgtgaagct gtcggcggaa gatatctggg atcgctgcgc ctatgttctg tcggttaaaa
    10801 tgcaggatcc gcagtttgcc gggcagacca aagagcgtct ctcttcgcgt cagtgtgcgg
    10861 cattcgtttc gggcgtggtg aaagatgcct tcatcctgtg gctgaaccag aacgttcagg
    10921 cggcggagtt gctggcagag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta
    10981 aaaaagtggt gcgtaaaaag ctgaccagcg gcccggcgct gcctggcaaa ctggctgatt
    11041 gtaccgcgca ggaccttaac cgtaccgaac tgttcctcgt ggaaggtgac tccgcaggcg
    11101 gatctgccaa gcaggcgcgc gatcgtgaat atcaggcgat catgccgctg aaaggtaaga
    11161 tccttaacac ctgggaagtt tcttccgacg aagtgctggc ctcgcaggaa gtgcatgata
    11221 tttcggtagc gatcggtatc gatcctgaca gcgacgatct gagccagctt cgttatggca
    11281 aaatctgtat cctcgcggat gcggactctg atggtctgca cattgccacg ctgctctgcg
    11341 ctttgttcgt aaaacacttc cgcgcgttgg tgaaacacgg tcacgtttac gtcgcactgc
    11401 caccgctcta ccgtattgat ctcgggaaag aggtttatta cgcgctgacg gaagaagaga
    11461 aagagggcgt acttgagcaa ttaaaacgca agaaaggcaa gccgaacgtc cagcgtttta
    11521 aaggtctggg ggaaatgaac ccgatgcaat tgcgtgaaac cacgcttgat ccgaacactc
    11581 gccgtctggt gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg
    11641 atatgctgct ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gagaaaggcg
    11701 acatggcgga gattgaggtt taaaggaaag aacattgccc ggtcaggatg gccgggcaaa
    11761 caaggcagga ttaaatccct ggctgcagaa tacggatatt catctccagc acgtcgcctt
    11821 ttacggcttt gctgtacgcc ttcatgtgcg gggtttgcag atgcgcttca aggtgcgcga
    11881 tgctttccca ctgctcaatc atcacgatag aatccggtgc catagactgg aaactcacgc
    11941 cagcagcgca atccaccatt ggcgcatagc cgtggcaacc ttcttctttc agtacggttg
    12001 gaacgatttt agcaaactga tccaataccg cctgacggtg atgttgacca ggacgagtac
    12061 ggatttctgc gattacggta agcatggtta actccttcta aagcctgagc tctagttaac
    12121 caaaaatttc cacaagatgc ttgcgatatt cttcagtata gcggggaaca tcaggcattt
    12181 ttatcacgtc attagcgata aatgtcggca gcggttccat accgaggaat tggtttgctt
    12241 tatggaacgg cagatacaca ccgtcaacgc caacgccgtg gaagaactga tctttttcgg
    12301 tgaaggcttc cattggtgcg ttccaggtta gagaaagcat atattttttg ccctgtacca
    12361 ggccgccgga accgtatttt ttcgacggat ctttgcgggt acgaccatcg ctggcataca
    12421 gagtcccgtg accttcggtg aatacatcat caatatattt tttcactgtc cacggcgcgc
    12481 ccatccacca gcctggcatc tgccagatca ccacatcagc ccagagaaag ttttgtactt
    12541 ccgctttgac attgtagtcg ctgtcggcgc gaacgatgcg gacatcatgc ccgaggtcgc
    12601 gcagtgtgcc atccgcgact tcggtcaggg tgtcgttcag ttgaccattg gagtgggcga
    12661 attttttcgc gccgttgata atcaggatgt tgctcatttt ttaacctcaa ggtgagacgt
    12721 ttgtcgacta tagtacgtca cagagcggta cggtgaaatt agcaaaatgt gcaaagtctt
    12781 ttgcgaattt agcaaaagtc tgatctagct ctgactcacc gatgcaggat aaatattttc
    12841 tgaaaatgac aatactgata gccattcctc ataattctta ctcgtaccgg aataatttac
    12901 acgtttggtt cggttttttt ggggattgaa aatatggctt tgaataagta aaagatgaaa
    12961 atatcaacgt gtcatgttga taattataaa tggattttta tgagcgtact gatatatgtt
    13021 aaagcagaaa attaaaacaa tatttgaagc attgctatat ataatgctta cttattggtt
    13081 aatagattct ttttttgctt tcaataagta tgactggatg cttgagtcgg gaggcaatat
    13141 ttgcagtata ccttccgttt caggcgaaga tcgtatattg caagcaatga ttgcagcatt
    13201 ttttcttctt acgccactta ttattcttat cttaagaaaa ctctttatgc gggaaatgtt
    13261 cgagttttgg ctatatgtat tctcgctcgt tatttgtctg gtttgtggtt ggtggctttt
    13321 ttggggacgg tttatatttt gctattagta aaatctatga gagtcgtttt aacggctctc
    13381 atagacagag aagttaccag cttaccttcg cctcaaatcc accttgttcc gcattcccaa
    13441 actcaacatt catgtcatgc agcttggcga ttcgctggac aatcgatagc ccaagtccac
    13501 tgccggtagc ggtttgtccg ggtgggcgat agaagcgttc gccaattcgc gccagtgcct
    13561 ctggtgtcac accggggccg ttatccctca cgatgaaatt atcagcattc agcgtgacgt
    13621 ctatcacgct gccctgtgga ctgtagcgca cggcgttatc cagcaaattt cgcaccaaca
    13681 aacttagcaa tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt gtcagtcgca
    13741 cgtcaatttt cgcctgctgc gccgtgtggt aaatatccat caccgacgat tgtaggagat
    13801 cttcaagcgg gatctccgcg acgtcctgaa ggttatccag tgagtccagc cgcgatagcg
    13861 tgagcagttg atcaaccaga cgagtagcgc gatcgatccc ggaatgtaat tggagcagtg
    13921 cttttttccg cgcctgcgga tcatcgtcag agagctgcgc aacttcggtt tgcactttca
    13981 gcgctgttaa cgggctacgt agttcgtgag ctgcatcgga ggtaaagcgt cgttctcgaa
    14041 ccatcatcgc atgtgtgcgg gcgaacagtt gatttagcga ctcaaccagt ggccgcactt
    14101 cgctgggtac gccagtcgcg tttagtggtt tttccgagtc agggtcacgc atacgtagtg
    14161 ccagcgccag tttgttcagc ggcgcgagtt cacgacccag tagtaccatc atgatgatta
    14221 acataatcgg cagtgcgacc agccacggga tcaattgccc ggcaacaatc gccagcgcca
    14281 tgtcttcacg gtattcccat tcctggccaa caacgatgcg atatttgcca tcaggtgagg
    14341 tcatccagac aaaacgccaa ggatcgtctt caccgaccag ttgcccgtca gcaaaacctt
    14401 cccgttgata gctataggga atatcttctc cgttatcgcc atcattaagg accattctgc
    14461 cgtcgtgggt aaagatggca aaggtcagcg catcgtcatc aacatgaccg tgttttaatt
    14521 tatttggcgt ctgtgccatg cgatccgccg cgttgatttc gttgagatcg agcgtactta
    14581 accgtttggc aaacagcatc agttgggtgt cgaacaattc atcgacgtta tccgttgttt
    14641 gtttccaggc gacaaagctg gaaagcagcc aggtcaccga ggccagaatt aaaaagatta
    14701 gcgtcagcct gacgcgcaga ctaagacgtt gggtaaattt catttctcac ctaatgtgta
    14761 gccaatacca tgcacggtac gaataaaatc actaccgagt ttgcgtcgca gatgatgcac
    14821 atgcacttca acggcattac tggtgacctc ttcgtcccag gtatacagtt tctcttcaat
    14881 cagtttgcgc ggcagtaccc gaccagcgtt acgcatcagt aattccagca gggcaaattc
    14941 ttttggtttc agtgttaagg gttcgccagc cagcgtggcg atacgtttgc cggggtcgag
    15001 catgacgtta ccgtggcgca gctcgttgct ggcctggccg ttggttcggc gcatcagagc
    15061 ctccagcctg gcggcgactt ctatcaacgc aaaaggttta cacagataat cgtcagctcc
    15121 cagacgcagc ccttctacac gttccgctaa cgcatcacgc gcggtcaggt tcagtaccgg
    15181 ctcacgctga cctttctctc gccattcgcg caaaatatcg cgaccatcca tgcctggtaa
    15241 ggttaaatcc aggatcaccg catcataagg tgcgctataa agcgcctctt ttccctgacg
    15301 accttgtgta aaccagtcga cactaaaacc cattttacta aggccagttt tgatgccgtc
    15361 accaatcagc atgtcatctt ctatcagtaa aattcgcatt ttttcatccc tgcgataacc
    15421 gtatttgctg ccagtaaacc gtgttgtaac gcgctctgta cagcattaaa ttttattttt
    15481 ttcccttaag aagttgttaa ggactatctt gttaaatgct cggaaacaga cattaaaggg
    15541 agtaataaac atgaaaaaat tcgcagcagt aatcgcagta atggccctgt gcagtgcacc
    15601 ggtgatggca gcagagcagg gcggtttttc tggcccatcg gcaacgcaaa gtcaggccgg
    15661 aggatttcag gggccgaacg gcagcgtaac gactgtagaa agcgtaaaat ccctgcgtga
    15721 cgacacctgg gtaaccctgc gcggcaatat cgttgaacgc atctctgacg atctctacgt
    15781 gtttaaagat gccagcggta ctatcaatgt tgatatcgat cacaaacgct ggaacggtgt
    15841 gacggtgacg ccgaaagata cggttgagat tcagggtgaa gtcgataaag actggaattc
    15901 tgttgaaatt gacgtcaaac agatccgcaa agtaaatccg taattgttac cgctcccggg
    15961 acgcgttccc gggaataatt tcgcagggag gcaaaatgac aaacctgaca ctggatgtaa
    16021 acattatcga tttcccatca atccctgtgg cgatgttgcc gcaccgctgt agccctgagt
    16081 tgctcaacta cagtgtggcg aaatttatca tgtggcgtaa agagacgggg ctttctcctg
    16141 ttaaccaaag ccagactttt ggcgtcgcct gggacgaccc tgccaccacc gcaccggaag
    16201 cgtttcgctt tgatatctgc ggcagcgtta gcgaaccgat tcccgataat cgttatggtg
    16261 tgagcaatgg tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt ggcgagctgg
    16321 acgatatttc acacacgata tggggcatta ttcgccactg gctgcctgca agcggcgaga
    16381 aaatgcgtaa agcaccgatt ctgtttcact acaccaatct tgccgaaggg gtgacagagc
    16441 agcgactgga aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt accggagtgt
    16501 gagccaaatc actcgcgata agtttgagcg aacaatgtca caggcgtatt gttgttgggc
    16561 ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatcc ggtgcgccgt
    16621 aagccgtctc gggggtatcc atgagcaagc cgcacttatg gcaggaggcg cggggcactg
    16681 gtggagacac agctaaactg ggctgaaccg tatacttaca gtcgactact ttgatgcttt
    16741 aggcttccct ggactcgcat aacctcaact tctcgaatcg cccggagcgg acccccatgc
    16801 cgagtggtgt ggcatgggcg caaccttgtg ctgccctcta tgccgatcaa gtctacagca
    16861 aagagttgta tatcaaggca cattgatgcc tttatcgccg tcggagcacg tccggcggcg
    16921 ctattcacgc tgatctcctg tgactcgacg cggcagataa tgtagtatct ccggcaatat
    16981 tgcccctttg aaggctggcg aataagttga ggaatcagaa ttaatgagcg atatggcaga
    17041 gcgccttgcg ctacatgaat ttacggaaaa cgcctactta aactactcca tgtacgtgat
    17101 catggaccgt gcgttgccgt ttattggtga tggtctgaaa cctgttcagc gccgcattgt
    17161 gtatgcgatg tctgaactgg gcctgaatgc cagagccaaa tttaaaaaat cggcccgtac
    17221 tgtcggtgac gtactgggta aataccatcc gcacggcgat agcgcctgtt atgaagcgat
    17281 ggtcctgatg gcgcagccgt tctcttaccg ttatccgctg gttgatggtc aggggaactg
    17341 gggcgcgccg gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat cccggttgtc
    17401 gaaatattcc gagctgctat tgagcgagct ggggcagggg acggctgact gggtgccaaa
    17461 cttcgacggc actttgcagg agccgaaaat gctacctgcc cgtctgccaa acattttgct
    17521 taacggcacc accggtattg ccgtcggcat ggcgaccgat attccaccgc ataacctgcg
    17581 tgaagtagct caggcggcaa tcgcattaat cgaccagccg aaaaccacgc tcgatcagct
    17641 gctggatatc gtgcaggggc cggattatcc gactgaagcg gaaattatca cttcgcgcgc
    17701 cgagattcgt aaaatctacg agaacggacg tggttcagtg cgtatgcgcg cggtgtggaa
    17761 gaaagaagat ggcgcggtgg ttatcagcgc attaccgcat caggtttcag gtgcgcgcgt
    17821 actggagcaa attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg acgatctgcg
    17881 cgatgaatct gaccacgaga acccgacccg tctggtgatt gtgccgcgtt ccaaccgcgt
    17941 ggatatggat caggtgatga accacctctt cgctaccacc gatctggaaa agagctatcg
    18001 cattaacctc aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc tgctggaaat
    18061 cctctccgaa tggctggtgt tccgtcgcga taccgtgcgc cgccgactga actatcgtct
    18121 ggagaaagtc ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt ttctcaacat
    18181 cgacgaagtg attgagatca ttcgtaacga agatgaaccg aaaccggcgc tgatgtcgcg
    18241 gtttggcctt acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc gtcatcttgc
    18301 caaactggaa gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag agcgcgacca
    18361 gttgcagggc attttggctt ccgagcgtaa aatgaataac ctgctgaaga aagaactgca
    18421 ggcagacgcg caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac gcgaagaagc
    18481 gaaagctatg agcgagcacg acatgctgcc gtctgaacct gtcaccattg tgctgtcgca
    18541 gatgggctgg gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc tgaattataa
    18601 agcgggtgat agcttcaaag cggcggtgaa aggtaagagt aaccaaccgg tagtgtttgt
    18661 tgattccacc ggtcgtagct atgccatcga cccgattacg ctgccgtcgg cgcgtggtca
    18721 gggcgaacca ctcaccggca aattaacgtt gccgcctggg gcgaccgttg accatatgct
    18781 gatggaaagc gacgatcaga aactgctgat ggcttccgat gcgggttacg gtttcgtctg
    18841 cacctttaac gatctggtgg cgcgtaaccg tgcgggtaag gctttgatca ccttaccgga
    18901 aaatgcccat gttatgccgc cggtggtgat tgaagatgct tccgatatgc tgctggcaat
    18961 cactcaggca ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc tgtcgaaggg
    19021 caaaggcaac aagattatca acattccatc ggcagaagcc gcacgtggcg aggatggtct
    19081 ggcgcaactg tacgttctgc cgccgcaaag cacgctgacc attcatgttg ggaaacgcaa
    19141 aattaaactg cgtccggaag agctacagaa agtcactggc gaacgtggac gccgcggtac
    19201 gttgatgcgc ggtttgcagc gtatcgatcg tgttgagatc gactctcctc gccgtgccag
    19261 cagcggtgat agcgaagagt aatatgccgg ggagggcgct gtttcctccc gctagcaagg
    19321 aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa
    19381 aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag
    19441 agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga
    19501 ccttcagagg gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc
    19561 ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg
    19621 gccacctttg ggcatatgtt tggccgtctt gcgccactgt tcggcctgaa agttgagtgc
    19681 cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac
    19741 aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa
    19801 aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg
    19861 atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc
    19921 aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc
    19981 ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt
    20041 cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg
    20101 gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt
    20161 gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa
    20221 gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa
    20281 cactttcctg cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc
    20341 ggcgtcagtt cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg
    20401 ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg
    20461 ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg
    20521 cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg
    20581 acgatgttaa gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg
    20641 ggctacaggt accaggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg
    20701 actgggcacc cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata
    20761 ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag
    20821 atgaagtcag caagtcgctg cctatcccca accattatgg tgtggatgat tttccggtca
    20881 ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg
    20941 gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct
    21001 cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac
    21061 agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc
    21121 ctgtatcggt gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata
    21181 tgagcaacgg cgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt gttgatcgta
    21241 ttcgcggctt ctttgagcca tccagtattc tggtttctac cttggtgcta acgctgcgcc
    21301 caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa
    21361 tcatggctgg ttcgccaatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta
    21421 atggacagct gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac
    21481 gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgta atgttccaga
    21541 tccgtaacgt gaatggcgcg atgccgttcc cggaagacag aggctggaaa gataccgttt
    21601 gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc
    21661 cgttctactt caacagtcag acgctggaaa tggcggaccg tggctcgatt gggcaactgt
    21721 tggtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacctt
    21781 tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc
    21841 tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc
    21901 gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat
    21961 ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga
    22021 tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg gctttcgcgt
    22081 cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc
    22141 gaatctgttt tttggtgtta ctgctggcaa catggattcg atgatcaacc gctataccgc
    22201 cgatcgccgt ttacgtcatg atgatgccta cacgccagat aatgtcgcgg gtaagcgacc
    22261 ggatcgcgcc acgctggttt atacccagcg ttgtaaagag gcgtggaaag atgtgccggt
    22321 gatcctcggc ggtattgagg ctagcctgcg ccgtaccgcg cattatgatt actggtccga
    22381 taccgtgcgc cgttccgttc tggtagattc gaaagccgac atgctgatgt ttggtaacgg
    22441 tgaacgtccg ctggtggaag tagcgcatcg tctggcgatg ggcgagccga ttagtgaaat
    22501 ccgcgatgtg cgtaataccg caattatcgt aaaagaggcg ctgcctggct ggagcggtgt
    22561 ggattccacc cgtcttgata ccccagggaa aatcgaccca atcccgcatc cgtatggcga
    22621 agatttgccg tgtgcggata acaaacctgt tgcgccgaaa aagcaggaag cgaaagctgt
    22681 aatcgtgcag ccaccgcgcc agaaaccgtg ggaaaaaacc tacgtattgc tgccttcttt
    22741 cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgcattc tgcaccacga
    22801 aaccaaccca ggctgcgccc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat
    22861 caacccgcct gctatcccgc tttctaccga agagatggac tgcgtttttg cgctgccgta
    22921 caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg
    22981 tttttcggtc aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga
    23041 gcacgaaggg cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc
    23101 gatccgcgac accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc
    23161 caacatgtat atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc
    23221 gtgcgtttat ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct
    23281 ctatcgccgt gcgcgtgatc tgaaaggcat taaaaaaatc cttatcgcct caggcgtacg
    23341 ttatgacatc gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg
    23401 cggttatctg aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa
    23461 gccgggcatg ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc
    23521 aggtaaagag cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga
    23581 agatatggtg aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca
    23641 gaacttctac ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc
    23701 gctggcgaag attggttata agagcgaaga cgtcttcgta ccgaagggcg acaaacagcg
    23761 tcgtttgcat aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgcca
    23821 ggcgctggaa gcgatgggca aaaagcatct gattggcagc ccgcgattgc ttagtgcctg
    23881 cgccaaccat tgaagagatg cgtgaagctc gtcgccagaa ccgcaatacc cgtccggcgt
    23941 tgactaaaca tacgccgatg gcgactcagc gtcagacgcc tgctacggca aaaaaagcgt
    24001 cgtctacgca atctcgtccg gtgaatgctg gtgcgaagaa acgccctaaa gcggcggttg
    24061 gacgttaaaa aaaatccccg gcaactgaca tgctgccggg gattttttta tcattctgcg
    24121 tacatacgca agcagcgcag gcaatcaaaa tagtcctgtg catcaagacg cgtattggct
    24181 ctgttagctt cggatttggc agcatagatt tgtgggcgta gtgctttcat atcctgctca
    24241 agccgggcga ttttggcatt taatgtttgt acattgctcg ccagttgatt gaccttttgc
    24301 tcattgacgc agccactgag caagcaggtt gccagaacaa cagctcccac ggtgaaaatc
    24361 gttttcatat agtgttccct tttatttatt gttccggcta attgttattt aattcagctc
    24421 attgctttaa tctggcggcg gtttttgcct caatcagctt aagtaggctg ccgctgttcg
    24481 ggcggataaa atcagcgatt tcccacttac cttcgcgccg ctgtaaaacc agctcttgct
    24541 cttcattgtt gccattggtc agcgtaaaac gaacatgaat gcgggcggaa tcggcggttt
    24601 tctccaggac tcgaatttca tcaacgcgtg aaaatagcga ctcttcgcca aaacagatcg
    24661 gattgtcgta aataaattgc cgttctattt gcatatcttc atcgggatta ttggccgtct
    24721 tgaaggtggt tttatagaag gcattaaccg cctggcggta agagtcggaa accacccacg
    24781 tccacggcca gctataatca gcaatatgtt caaagagttc agcaacaaag gattctggct
    24841 gttctttctg caacgaagct aaagcggcca gcgttttttc attctcgcta ttaactgctt
    24901 gtaagacgct gccatgattg ctgacaatat catcaatgac ccaacgacca ttttccgcca
    24961 ccattttcag tgtctgtgtg gtcttttctt tatcatcttt aaagatacga aagcgcacaa
    25021 cggcatcggc atgatcgacg tcagtttggg ttatcgctac gctttctaac accagatcgc
    25081 caaaatcctg acaatcacaa accggatcat aatccagcca gccaatattg cccggtagtg
    25141 taagattgtt gttcagggta agcgcctgtt gaatacgcgc tgaagttatc gcccgctctc
    25201 cggtttcacc gaaataaggc gtactggcat ctgatttata gttctgataa atctgccgga
    25261 ctgtttgttc tacggtctgt gaaggaggct gcgcgtgtac ggtaaaactt gccagggctg
    25321 ctaaaaacag aagtattatt ttcatctgtt tttcacctct ccgttgcgtt tatcccggat
    25381 atagatgagt tgttctttta agagcttccg gctctgcatg atgatgtcct tatatttggc
    25441 attcctgaac aaggaagagt aacaacgggc gggacgcgag gggaataaat gatttctgaa
    25501 aagtccggta gcggaacatt accgccaccg ggagaatttg cacgttagcc gccgaactgg
    25561 tcaggatcgg gaccgagacg cttgccctga tcgagttttg caattttgcc gagttcgtct
    25621 ttgtcgagac ggaaatccca gacatcaaag ttttcggcaa tacgtgaagg tgtgaccgat
    25681 ttcgggatca ccaccaggcc gctatccaga tgccagcgga taacaatctg cgccggggtt
    25741 ttgccgtatt tatctgccag atcgcgaatg actttctgat cgaaaacgcc ttttcctcct
    25801 tgcgctaatg ggctccagga ttcggtctgg attttgtgtg tcgcgttcca ggcgtgtagc
    25861 tggcgttgtt gcatcagcgg atgaagttcg atctggttta tcacaggcgt cacgccagtt
    25921 tcatcaatca ggcgttgcag gtgatggatc tggaagttgc acacgccgat gcttttgatt
    25981 aatccctctt tttgcaattc gatcatgcct ttccatgctt cgacataatg gtcgatagcg
    26041 ggaacgggcc agtgcattaa gtagaggtcg atataatcaa gctggagttt tttcaggctg
    26101 tcgagcaggg cttcgcgggg gcacttgtgg tcgtcgttcc acagcttagt ggtgatgaac
    26161 agttcttctc tgttgactga ggcatttttc agggctttgc cgacaccttc ttcgttcttg
    26221 taggccgcgg cggtatcaat cgagcgataa cccacttcta acgctttttg aatggcggtg
    26281 attacttcct cattacttgc ttgccagacg cccagtccca gctggggcat gacattgcca
    26341 tcctgtagct taataacggt tggattagcc atacgttcct cctttatatg aactcaccgg
    26401 agcaggctcc ggtgaggtgt tgtgaactta agtctggacg aaatgcccga aaacgaaagt
    26461 ttgaggcgta aaaagcttag cgggcggctt tgtatatacg gcggctgaca tccaacgtaa
    26521 tgtcatgatt ttcgcccagt tgggtcatgc cgtgctcttc cagttttttc agcaaagccg
    26581 ggatggagct gccgtccaga ccgtagtcgg agaggtgggt cggcacgcct aattgctcaa
    26641 agaaattgcg ggttgcggca atcgcggcgt caatacgctc atcgtcggaa ccttcagtga
    26701 tgttccagac gcgttcagca tattgcagca gcttagcgcg cttggtctcg cgtttttcat
    26761 tccacagtgc aggcaggacg atagccagtg tttgcgcgtg atccagaccg tgcatcgcag
    26821 tcagttcgtg gcccagcata tgcgttgccc agtcctgcgg tacgccagcg ccaatcaaac
    26881 cgttcagcgc ctgagtcgcc gcccacatga cgttggcgcg cacatcgtag ttttctggct
    26941 ctttcagggc tttcggacca tcttcgatta gcgtcagcaa aatgccttct gcgaaacggt
    27001 cctgaatttt ggcatcaacc ggtttggtaa catactgttc cacggtgtgt acaaaggcgt
    27061 ccactacgcc gttagccacc tgacgcggcg gcagggtgta ggtataaacc ggatcgagca
    27121 cggcaaatac cggctgaaca tgtgcagaat ggaacgcctg cttgtcgcct gtggttttac
    27181 gggagatcac cgcgcctgcg ttggattctg aaccggttgc tggcagcgtc agcacacagc
    27241 ccatcgggat ggcgctttta atctctttac cgcccgtttg cagaatgtgc cacggatcga
    27301 tattttccgg atagttagcc gctgcggcga taaatttggt gccgtccagt acagaaccgc
    27361 cgccaaccgc cagcaggaaa gtcactttct gttcgcgaac cagtttcacg gcgttcatca
    27421 gcgtttcata agccgggttt ggctcaatac cgccaaattc cagcacgtcc atgcctttca
    27481 gggcatccag aacttgatcg agaacgccgg tttttttcac gctgccgcca ccgtaggtaa
    27541 tcaatacgcg agcatcgtga ggaatttgtt cgcgtaaacc agcgattgcg cctttaccaa
    27601 acagaatgcg ggttggggtg tgcagattaa agttgttcat tacttgctcc ctttgctggg
    27661 ccaatatgag ggcagagaac gatctgcctg atgtttttca ttgtgatcgc cagcgcccta
    27721 gctctcaatg ctcatttctg ccaatgtctt gcctatttct ccagagtgct ggagaaatgc
    27781 tacaaaattg cgcacaatca aattgccgca ttattcctaa gaaattacgc gatatgaaac
    27841 gtgaagagat ttgtcgcttg ctggcggata aagttaataa actgaaaaat aaagaaaata
    27901 gtttgtcaga actgttgccc gatgtgcgtt tgttgtatgg cgagacgcct ttcgcacgta
    27961 caccggtgat gtacgagcct ggcatcataa ttctcttttc cgggcataaa atcggttata
    28021 tcaat
//
LOCUS       DAJTRQ010000038        27453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000038 DAJTRQ010000000
VERSION     DAJTRQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO332_001766"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO332_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5942272.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO332_001767"
     CDS             complement(1614..1937)
                     /locus_tag="OO332_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5942273.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO332_001768"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO332_001768"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5942274.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO332_001769"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO332_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5942275.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO332_001770"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO332_001770"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5942276.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO332_001771"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO332_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5942277.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO332_001772"
     CDS             7537..8574
                     /locus_tag="OO332_001772"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5942278.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO332_001773"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO332_001773"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5942279.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO332_001774"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO332_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5942280.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO332_001775"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO332_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5942281.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO332_001776"
     CDS             10745..11626
                     /locus_tag="OO332_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5942282.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO332_001777"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO332_001777"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5942283.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO332_001778"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO332_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5942284.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO332_001779"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO332_001779"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5942285.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO332_001780"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO332_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5942286.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO332_001781"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO332_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5942287.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO332_001782"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO332_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5942288.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO332_001783"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO332_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5942289.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO332_001784"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO332_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5942290.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO332_001785"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO332_001785"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5942291.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO332_001786"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO332_001786"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5942292.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO332_001787"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO332_001787"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5942293.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO332_001788"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO332_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5942294.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO332_001789"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO332_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5942295.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO332_001790"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO332_001790"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5942296.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO332_001791"
     CDS             24893..25273
                     /locus_tag="OO332_001791"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5942297.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO332_001792"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO332_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5942298.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO332_001793"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO332_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5942299.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27453
                     /locus_tag="OO332_001794"
     CDS             26442..>27453
                     /locus_tag="OO332_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5942300.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVF"
BASE COUNT         6768 a         6835 c         7200 g         6650 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttt
//
LOCUS       DAJTRQ010000039        27441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000039 DAJTRQ010000000
VERSION     DAJTRQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..802)
                     /gene="ycjM"
                     /locus_tag="OO332_001795"
     CDS             complement(<1..802)
                     /gene="ycjM"
                     /locus_tag="OO332_001795"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5942301.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTV"
     gene            complement(1014..1361)
                     /gene="pspE"
                     /locus_tag="OO332_001796"
     CDS             complement(1014..1361)
                     /gene="pspE"
                     /locus_tag="OO332_001796"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5942302.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1404..1625)
                     /gene="pspD"
                     /locus_tag="OO332_001797"
     CDS             complement(1404..1625)
                     /gene="pspD"
                     /locus_tag="OO332_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5942303.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1634..1993)
                     /gene="pspC"
                     /locus_tag="OO332_001798"
     CDS             complement(1634..1993)
                     /gene="pspC"
                     /locus_tag="OO332_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5942304.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1993..2217)
                     /gene="pspB"
                     /locus_tag="OO332_001799"
     CDS             complement(1993..2217)
                     /gene="pspB"
                     /locus_tag="OO332_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5942305.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2271..2939)
                     /gene="pspA"
                     /locus_tag="OO332_001800"
     CDS             complement(2271..2939)
                     /gene="pspA"
                     /locus_tag="OO332_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5942306.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3106..4083
                     /gene="pspF"
                     /locus_tag="OO332_001801"
     CDS             3106..4083
                     /gene="pspF"
                     /locus_tag="OO332_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5942307.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4203..5468)
                     /gene="puuE"
                     /locus_tag="OO332_001802"
     CDS             complement(4203..5468)
                     /gene="puuE"
                     /locus_tag="OO332_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5942308.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5506..6786)
                     /gene="puuB"
                     /locus_tag="OO332_001803"
     CDS             complement(5506..6786)
                     /gene="puuB"
                     /locus_tag="OO332_001803"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5942309.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6788..8276)
                     /gene="puuC"
                     /locus_tag="OO332_001804"
                     /pseudo
     CDS             complement(6788..8276)
                     /gene="puuC"
                     /locus_tag="OO332_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8426..8983)
                     /gene="puuR"
                     /locus_tag="OO332_001805"
     CDS             complement(8426..8983)
                     /gene="puuR"
                     /locus_tag="OO332_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5942310.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9010..9774)
                     /gene="puuD"
                     /locus_tag="OO332_001806"
     CDS             complement(9010..9774)
                     /gene="puuD"
                     /locus_tag="OO332_001806"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5942311.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9986..11404
                     /gene="puuA"
                     /locus_tag="OO332_001807"
     CDS             9986..11404
                     /gene="puuA"
                     /locus_tag="OO332_001807"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5942312.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11553..11717
                     /locus_tag="OO332_001808"
     CDS             11553..11717
                     /locus_tag="OO332_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942313.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11707..13092
                     /gene="puuP"
                     /locus_tag="OO332_001809"
     CDS             11707..13092
                     /gene="puuP"
                     /locus_tag="OO332_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5942314.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13226..13471
                     /locus_tag="OO332_001810"
     CDS             13226..13471
                     /locus_tag="OO332_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5942315.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13784..15427
                     /gene="sapA"
                     /locus_tag="OO332_001811"
     CDS             13784..15427
                     /gene="sapA"
                     /locus_tag="OO332_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5942316.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15424..16389
                     /gene="sapB"
                     /locus_tag="OO332_001812"
     CDS             15424..16389
                     /gene="sapB"
                     /locus_tag="OO332_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5942317.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16376..17266
                     /gene="sapC"
                     /locus_tag="OO332_001813"
     CDS             16376..17266
                     /gene="sapC"
                     /locus_tag="OO332_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5942318.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17266..18258
                     /gene="sapD"
                     /locus_tag="OO332_001814"
     CDS             17266..18258
                     /gene="sapD"
                     /locus_tag="OO332_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5942319.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18260..19066
                     /gene="sapF"
                     /locus_tag="OO332_001815"
     CDS             18260..19066
                     /gene="sapF"
                     /locus_tag="OO332_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5942320.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19134..19487
                     /locus_tag="OO332_001816"
     CDS             19134..19487
                     /locus_tag="OO332_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5942321.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19855..20643
                     /gene="fabI"
                     /locus_tag="OO332_001817"
     CDS             19855..20643
                     /gene="fabI"
                     /locus_tag="OO332_001817"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5942322.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20789..21916
                     /locus_tag="OO332_001818"
     CDS             20789..21916
                     /locus_tag="OO332_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5942323.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21984..23918
                     /locus_tag="OO332_001819"
     CDS             21984..23918
                     /locus_tag="OO332_001819"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5942324.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23996..24098)
                     /locus_tag="OO332_001820"
                     /pseudo
     CDS             complement(23996..24098)
                     /locus_tag="OO332_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24152..26126
                     /gene="pdeR"
                     /locus_tag="OO332_001821"
                     /pseudo
     CDS             24152..26126
                     /gene="pdeR"
                     /locus_tag="OO332_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26274..26447
                     /locus_tag="OO332_001822"
     CDS             26274..26447
                     /locus_tag="OO332_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942325.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26537..27286
                     /locus_tag="OO332_001823"
     CDS             26537..27286
                     /locus_tag="OO332_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5942326.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6365 a         7114 c         7047 g         6915 t
ORIGIN      
        1 tcactgtacc tggtgctacg tcatcaataa tcgaccgtaa cagcttgata atcagatgtg
       61 ttttttccag atggatgcag cttgttcccg gctctttcca cataaagcca acggcatcca
      121 ggcggacata ctcagcacct ttctcaaggt aacagagtaa aacatccacc atcgccagca
      181 acacttcagg gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg gtccataaat
      241 ggcgcgtgga attatcatcc atttgaaacg gcgttaatag cggtaacgca cgcggacggg
      301 tgacggcact gagatcggtt ttcggatcaa ccgcaataaa gaaatcttca aaacccggaa
      361 gctgttgtaa atagttttta aaccattcac tttttgcaga catatggttg cagacaaaat
      421 caaacattaa atgactgcat tcaccgagtt gctgaatatc ctgccactcc cccgcttcac
      481 tggcgacctg atgataatca attaccgaaa atccatcatc agatgaccat gggtaaaaag
      541 gtaataaatg aacatgtgaa aaaatgcttt gcagccattg atggtagaac tgattaaata
      601 tgggtagtgg ttttaaatca ttactgtgaa attgatcggc ataggtaatt aacacgacat
      661 cactttcatc ccagtgtttt ttacgtcgct gtgtaattaa tcgtttcgca ctctcaagac
      721 gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt tcgtccaggt
      781 aatccgtaat tttctgtttc atgttgcctt tccgtggtag cggtcccatg aggcgagaga
      841 ctactccgca gaaaataaac tgtcaattga gcacaagggt tttagtcacg caggaaatca
      901 ttttttacca gggaaaaagc gtatttgtga gctgacgcaa ataatctata gggatgtaaa
      961 taaggtgtga cagaaaaaaa acggcgcata agcgccgctc atggtgaatt cttttaacct
     1021 ttgaccttcg gcattgcgat gtctttcagg ccaccggcgt tctcaacgtg ggtatatccc
     1081 atctcgctaa ggatctcttt tgcctgcccg gactggcgtc cggcattgca atacactttc
     1141 acggtgtcgt ttttatccgg aactgcggtg gcaatgcgct ctttcacttc cttcagggga
     1201 atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg aacatcgatc
     1261 cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag taagcctttt
     1321 tttaaacatg taagcgtcct tctatggatg aaaattgcca tccattatac gcctgagatt
     1381 taacaatttt tgcgaaactc ccctcacctt ttataacgct gtgccagttt attagcagcc
     1441 cgacttaaca gcggttccag cgccacagcc agcaacattt tcagcggtcg gcgagcaact
     1501 gattttatcg cccagcccgc cacacccgcc gggccatagc gcagtgcggt aagaagtacc
     1561 agcttgcctg ctaatttgaa accaagcttc accttttgcc cggcctgttg ccagcgagta
     1621 ttcataactt tcctcacagt tgacggaaac ggctacgtaa cgtgaaagta tcggaagtga
     1681 cataacgttc catctcgcgt aaacgcgttt cacttgccgc cagttcacgg tcgacttcat
     1741 ccagcaattc gctgctggaa ggtagctgct caccaaaggc catgttgtcc ggcattggat
     1801 caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa cagcgccaga ccgaagaaaa
     1861 tcgacagcac caccaggata cgcaccagtt ttaccggtac atcaaaatag ttggcaatcc
     1921 cggcgcagac gccgcggacc atgccctgct gtggaatacg ccataatttt ttattgagat
     1981 taatgcccgc cattagcgat ccctccagtt cggatgttct gcatcaagaa tagattccag
     2041 cgcctgaata cgttcgcgca tccgttttgc ttcatcaacc agttgcgcta atcgctgctg
     2101 ctcactttgc gacaattcac tgcgaccaga acgattgctg taatgcagcc ataaccagat
     2161 cggtaaaaca aacagcacaa aaatggttaa cggaatagcc agaaatagcg cgctcataag
     2221 tactccttag acgatgagcg gcggcgcgtt cggacgccgc ctggatgtta ttattgattg
     2281 tcttgcttca ttttggcttt taattgtgcc agttgttcgc tgattgcatc atcggctttc
     2341 agttcggcaa actgatcgtc cagcgatttt tgtttaccga agctgtggct ttctgcttcc
     2401 gcttccatct ggtcaatacg acgttcgaaa gattcgaaac gagccattgc ttcatccagt
     2461 ttgccactgt ccagctgacg acgcacatcg cgcgacgagt ttgccgcctg atgacgtaac
     2521 atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt tttccagctc gccaatctct
     2581 ttcttcatgc gtgccagcgt atcgtccacc agcgtcactt catgttccag ggacttaatc
     2641 agatcggtca gtttctgttt ttcaattaac gctgcacgtg ccagatcctc tctctctttc
     2701 agcagcgcca gttcggcttt ttcctgccat tcaacttcac gcgccgacgc ttgttcaata
     2761 cggcgagtca gctgtttctt ttctgccaac gcacgcgccg aagtagaacg tacttcaacc
     2821 agtgtatctt ccatctcctg gatcatcaga cgcaccagtt tctgtggatc ttccgctttc
     2881 tctaacagag cgttgatgtt ggcattcacg atgtcggcaa agcgagaaaa aatacccata
     2941 atgttgtcct cttgatttct gcgttcagga ttgtcctgct gaactgttaa tacaatttgc
     3001 gtgccaattt tttatctttt tgatttataa agatctgatt gaagaatcaa cagcaacatg
     3061 ccaggatgaa ttagcgaatt acactaacaa gtggcgaatt tcatcatggc cgaatacaaa
     3121 gataatttac ttggtgaggc gaacagcttt ctcgaagtgc tggaacaggt ttcgcatctc
     3181 gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg gcaccggtaa agagttgatt
     3241 gccagccgcc tgcattatct ctcctcccgt tggcaagggc cgtttatttc ccttaactgc
     3301 gcgacgttaa atgaaaatct gctggattcc gaactgtttg gtcacgaagc gggggcgttt
     3361 accggtgcgc aaaaacgtca tcctgggaga tttgaacgtg ccgacggcgg tacgctattt
     3421 cttgatgaac tcgctacggc accgatgatg gtgcaggaaa aattattgcg tgtgattgag
     3481 tacggtgaac tggagcgcgt tggcggtagc cagccgttgc aggtgaatgt gcggttggta
     3541 tgcgcgacga atgccgatct cccggcgatg gtcaatgaag gtacttttcg cgctgacctg
     3601 ctcgaccggc tggctttcga tgtagtgcaa ctgccgcccc tgcgcgagcg cgaaagcgac
     3661 attatgctga tggcagaaca ctttgccatt cagatgtgtc gggaaatcaa gctgcctctg
     3721 ttcccggggt ttacggagcg cgccagagaa acattgctga attatcgttg gccgggaaat
     3781 attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc gccacggcac cagcgattat
     3841 ccgcttgatg acatcattat tgatcccttt aaacggcgtc cacctgaaga agccatcgcc
     3901 gtttcagaaa atacctcgct tccgacgctg ccgctggatt tacgcgagtt tcagatgcag
     3961 caggaaaaag agttgctgca actcagtttg caacagggga aatataacca gaagcgcgcg
     4021 gctgaattac tggggttaac ttatcatcag ttccgcgcgt tgttgaaaaa gcaccagatt
     4081 tagcgaagtt gagcaataca acttgccgga tgcggcgtga atgctttatt cggcatacat
     4141 aatcaatacg tcgtttgcag gcctaataag cgcagcgcat cagacattat tgcgttgggc
     4201 tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg cgcatccggg
     4261 atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg cgccacaggt cagcaggagc
     4321 agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc
     4381 gtttgcggat cgttaaactc tgccgcaatc atcgacccca ggccgcgtac cgcagcaatg
     4441 gccggaacgc tttctttggc atcaatcaac gtgtttttga gacgctggcc cagttgattc
     4501 gcgcgttcgc agagtgattc tttgtcgata atattgagca ccgcgtgcgc ggcagccacc
     4561 gccagcgggt tcccggcgta ggtgccaccc aacccgcccg gcgcgggcgc gtccataata
     4621 ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc
     4681 attaagtccg gcttatcggc gtaatgatcc atggcaaaca gtttaccggt acgcgcaaag
     4741 ccgctttgca cttcatcggc aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg
     4801 gcggcaacca gctcttttgg cgcaacgttg aaaccgccct cgccctgcac cggttcgaaa
     4861 ataatggccg cgacctgctt cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg
     4921 agggagtcct gtgttgaaat gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa
     4981 ccagggaacg ggccgaagcc gattttgtac ggcgcaactt ttccggtcag cgccatggtc
     5041 atatatgtac gaccgtggaa gccaccgcta aacgcaatca cgccaggtcg tccggtatgg
     5101 gcgcgagcaa ttttcaccgc gttttccacc gcttccgcgc cggtagtgaa gaatgcggtt
     5161 ttagcctgcc cgctcactgg ggcaagggcg ttaattttct ccgccagcgt gacgtagctt
     5221 tcgtacggca caatctgata cgccgtgtgg gtaaactgtt gcaattgctg ctccaccgcc
     5281 gcgaccagat cagagtggcg atgcccggta ttcagcaccg caatgcctgc ggcgaaatcg
     5341 atgtattcgt tgccctctac gtccttcagc gtagcgtttt ctgccgactg ggcgaagaag
     5401 ttacacatca cgccaacccc gcgtggggta gcagaaagac gacgctgatg gaattcattg
     5461 ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa cgcccagacg
     5521 atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaat ggcacgcgca gcgtgcgccc
     5581 gccggggaac gggtaatgcg gcagattggc gaaggcatcg aaacgctcgg cgtcaccgcg
     5641 cagcagttca gcaatcaaac gcccggcaag atgggtacag gtgacgccgt ggccgctgta
     5701 accctgcatg taatagatgt tggtatcaag gcggccgaac tgcggcatgc gcgacaaggt
     5761 cagcaggaag ttgcccgtcc agcggtaatc aattttcacg cccttcaact gagggaaggt
     5821 ttttagtagt ttcggtacca caagacgctc aacgtcatcc gggttgcgtg caccatacac
     5881 tacacccccg ccgtacagca ggcggttgtc ggcggtaaga cggtagtaat caagcagata
     5941 gttacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat cttccgacag
     6001 ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg cgtttcgcta gttccggctc
     6061 tactttatcg cccagatacg cattcccggc gacaatcaca tacttcgccg tcacctgacc
     6121 attggcagtc cgaaccacgg ctggcgtggt gtgctggatc tgcgtcacgg cggaaagctc
     6181 atacacgcgc ccgccgttca ggcggatggc atcggcttca ccaatggcga ggtttagcgg
     6241 gtgaatatgc ccaccgctgt gatccagcag cgcaccagtg tagcgatcgc tggcgacctc
     6301 gcggcgaata gcgttggcgt ccagcagctc cagctgttta ttgccgtagc gttcccagtt
     6361 ctctttctgc tcttcgagtg tggcgagttg tttatcattc atcgccacaa acagaccgcc
     6421 ggggcggtag tcgcagtcaa tctgataacg tttgatacgt tcgcggatga tctcgccacc
     6481 ctcgaacatc atgctgccga gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac
     6541 gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca ttgcgcccgc ttgcgccaaa
     6601 gccgatgcgt gaggcttcga gaaccactac gtcaaagccc gcttccgcca gatgcagcgc
     6661 ggaggagagc ccggtatagc cgccgccaac cacgcaaacg tcgcaggtga ttgactcatt
     6721 cagtgtatcg aatggtgcat atttattcgc actggcggcg tagtaactgc tggtatgttc
     6781 ggtcatttca ggcctccagg cttatccaga tggttttcag ttcagtgaat ttttcaaggg
     6841 catgcaggga tttgtcgcga ccgttgccgc tctgcttata gccgccaaac ggcacggtca
     6901 tatcgccgtc gttgtagtta ttgacgaaga cggaaccggc tttcaggcgt cggctcatgc
     6961 ggtgcgcgcg ggagaggtcg cgcgtccata ccgccgcgcc aaggccgtac tggctgtcgt
     7021 tggcaagctg cagcgcctga tcttctgatg tgaaacgggt gaccaccagc accggaccga
     7081 aaatctcttc gcggcttaag gacgcattcg ggtccacatc cacgaagatg gtcgggccga
     7141 tggccgctgc cagcccggcg ttgcgaccat ccagcagcag ttgccccttg ccttcgcctt
     7201 cacgaataaa ggtgtgtacc gagtcagcgt gagcgctgtc gattaaggtg cccatggtgg
     7261 ttgcgggatc aagtggatgg cccggctgcc agttttgcgc ctgctgtttt aacagggcta
     7321 agaattcatc ggcgatgctc tcttccagta acaaacgcgt tccggcgatg cacacctggc
     7381 cctggttgta gaagatgcct gcggcggtgg cgcttgccgc tttttgcaga tccggacaat
     7441 cggcgaaaac gatgttggcg cttttgccgc ccgcttccag ccagacacgt ttcatgttgc
     7501 tatcacccgc gtctttcagc agttgtttac ccgtacgcgt ggagccggta aaggcgatgg
     7561 catcgatatc gttatgacgc gaaagcgcct gccccgcttc atgaccaaaa cccgtcacca
     7621 cgttcaacac accatccggc aagcctgctt ctttcgccag ccccgcgaga cgaatcgcac
     7681 tgagcggtga tttttcagac ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc
     7741 cgagtttcca gcaagtcagc aacagcggga agttccacgg cacgatggcg gcaatcacgc
     7801 cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc acttcgccat
     7861 acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat
     7921 cacgcagact gtgacgaatc cggtttgccg gtgtcgagag tttccagcag tgccagctct
     7981 tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga
     8041 gaagagagtg accagtcgcc gcgttcaaat acgccgcgag ctgcgctcac cgcacggtcg
     8101 atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac cggatcaacg
     8161 gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt
     8221 tcaatggcga ggcttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg
     8281 actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc
     8341 cgggctgcat gcccgtttat ttctggcgac attctttatg gattgccggg cgcattgcgc
     8401 ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg ctgataattc
     8461 ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta atggcataac
     8521 tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt tcaccttcca
     8581 gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt gtgcccggct
     8641 ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt aacgttatga accagcttca
     8701 ttgacacccc ctgactgccc atctcaatta agtcgtcctg attaatgacg acctgcggct
     8761 catcaggttt ttccggctcg gaaaagaatt ccgagagtga cagaccatac accttcagca
     8821 gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta ctgatagcac
     8881 tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc tgctggcgga
     8941 tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca tttccttaaa
     9001 ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgccaagcgg tgataaagcc
     9061 ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt gccactgtac
     9121 gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat ccggcgagcg
     9181 agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgta gagagtttac
     9241 ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt caacctgaac
     9301 ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc gatgctccag
     9361 caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca ccaccagttc
     9421 ttgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg cattaattat
     9481 cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt tttcaccata
     9541 taggtgcggc tgcacattgc tgggactact aggaagataa atgccatcga gtttcggcaa
     9601 aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg gcaagccgcc
     9661 tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat gacccttaag
     9721 cctgttcctg cacattacga caccgataac cggattgttc attatatttt ccatgctcaa
     9781 tctcacaaag tggactaaat tatcgccatt actgcgcttt aaatgacctt tatgttcaat
     9841 attttttcaa tctagcagtg gattttcatt tttgcaaact aaatttaaca tttgacaaac
     9901 atttggtttg catacagatt cgaatgatgg tcattatatt ttacgctttg ataacgagcg
     9961 gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag agaactttgt
    10021 tcagcagtca gaagagaggc ggggtagcgc ctttacgcag gaagtgaaac gctacctgga
    10081 gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg gttgcttccg
    10141 tggaaaacgt atccctgttt caagcctgaa gaagctcgag aaagggtgtt atttcccggc
    10201 ctcggtattt gcaatggata ttctgggcaa cgtagtagaa gaagcaggtc tgggtcagga
    10261 aatgggcgag ccggatcgta cctgtgttcc tgttctcggt tccttaactc cttctgccgc
    10321 cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg gcgctccctt
    10381 tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctgcgcc agcgcggatt
    10441 gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgccagc gcgacgctga
    10501 aggggatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac aaagccaggt
    10561 ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg atgaactggc
    10621 gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg gtcagtttga
    10681 aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc tggcactaaa
    10741 acgcctggtg cgtctgatgg cagaaaagca taagatgcac gccactttta tggcgaagcc
    10801 gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa ataatcgtgg
    10861 cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa agatgctcgc
    10921 cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg tgaactcgta
    10981 tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc ataacaaccg
    11041 caccgtcgcc ctgcgtattc cgtgcggtga ccgtcataat caccgcgtgg aatatcacgt
    11101 ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg gtattttgca
    11161 tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc tggaacagga
    11221 aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg agaatgatca
    11281 cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta aaaatgatga
    11341 gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc
    11401 gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg acatttgctg
    11461 gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt
    11521 aacgattaag actgtgacaa ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg
    11581 agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct ttgcttccct
    11641 tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc aggggaggca
    11701 gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa aacccgtctg
    11761 cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct cacgccgatg
    11821 accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt tccggcgtcc
    11881 tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa actggttcgc
    11941 cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa cccgcacgtc
    12001 ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat gatcaacgtt
    12061 ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg
    12121 gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac
    12181 ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat cttcctggtg
    12241 gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc
    12301 agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg tttctcgttc
    12361 ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc acgcgtgatc
    12421 ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc ggcgtcgttc
    12481 tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc cgcactgcct
    12541 gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg caccacgttt
    12601 gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct gctgtatgtg
    12661 atggggcgtg acaatgtgtt tccagagcgc gtgtttggct atgtgcaccc aaaatggcgg
    12721 actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct gttctttgat
    12781 ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac cttcgtgaat
    12841 ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg gaaggatcac
    12901 ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct gtgggttaac
    12961 ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg cgcatatttg
    13021 tggtacctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag aacgccggtg
    13081 agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa cgcgccgcgc
    13141 cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc ccttatgctt
    13201 agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat attttgatat
    13261 tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac gcacaccgct
    13321 ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga tcagcgaaga
    13381 agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg atgagatcgc
    13441 agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat cggtcagtat
    13501 cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca ctgcagcgtg
    13561 ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta
    13621 tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac ctgttcaacg
    13681 accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca aatgtcatta
    13741 aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc aggtattatc
    13801 gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc
    13861 cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc aagtcaacac
    13921 ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg cccagtttta
    13981 tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac ttgccgaaag
    14041 ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt
    14101 tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg tggtgtttac
    14161 cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca gcaacttccc
    14221 ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac tggataatca
    14281 taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc tcgcaaccca
    14341 ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag atcgccagga
    14401 gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat accgcgccgg
    14461 gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt taatgccgca
    14521 ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc tgaccgggga
    14581 atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg acgacccgcg
    14641 cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta acaccgccaa
    14701 accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta ataaccagcg
    14761 cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt taccgcgcgc
    14821 ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga aatcgcgcga
    14881 acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc ccacacgttc
    14941 gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg atatggcgca
    15001 ggttgacgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg cgcggttgat
    15061 ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg acccggacag
    15121 tttcttccgt ccgttactga gctgcgcggc aattcattca cagaccaacc tcgcccactg
    15181 gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc agctggcagc
    15241 gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac ctattctgcc
    15301 gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc tggtacttag
    15361 cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa
    15421 accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt gttcctgctg
    15481 accttcgttg gcttcagctt gagctatttc actccacacg cgccgttaca aggcgcgtca
    15541 ctgtggaatg cctgggtgtt ctggtttaat ggcctgatcc actgggattt tggtgtttcc
    15601 agcattaacg gtcaaccaat tgccgaacag ttaaaagagg tcttcccggc gacgatagaa
    15661 ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg gatgattgcc
    15721 gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt actgggcttt
    15781 tcaatcccgg tgttctggct ggcgcttctg ttgacactgt tttgttcact cacgctaggt
    15841 tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat taccggtttt
    15901 gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat catgagcgca
    15961 atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc
    16021 cgcctgatgc gtatcagcac tattgaagtg tatgaccaga actatgtaaa agcggcggcg
    16081 acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa cgcgttaccg
    16141 ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc gatgatcacc
    16201 gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat ccgccagcag
    16261 gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat tattgttaac
    16321 gtgatttctg atattttggg tgccatggct aaccctctga aacataagga atggtatgcc
    16381 ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa
    16441 attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcgggac tggctgtact
    16501 gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc tcggttatca
    16561 attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg ggactgacga
    16621 cctcggacgt gatgttttaa gccgtttact gagcggagct gcgccaaccg tgggtggcgc
    16681 atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct tcgccggggc
    16741 gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac tggcgatccc
    16801 ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt cccacgctat
    16861 gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca gcatggtgca
    16921 tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat caacgctgaa
    16981 tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg agatcacccg
    17041 cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca
    17101 actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga tttatgtcgc
    17161 cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt tggttaacct
    17221 ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc attactcgat
    17281 attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgg
    17341 gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc cggttccggc
    17401 aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg tgttactgct
    17461 gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga acggcgcaaa
    17521 ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct tgacccttca
    17581 gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa aggccgttgg
    17641 tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg cgtggggatt
    17701 aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag
    17761 aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc tgacgaaccg
    17821 acaaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac gcgtctcaac
    17881 caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct tagccaatgg
    17941 gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc gagtaaggag
    18001 ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat accagacttc
    18061 ggcagcgcga tgccgcataa aagtcgcctc aatactctac ccggcgctat cccattgctg
    18121 gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca acgagaatgc
    18181 attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca tttcccgctg
    18241 aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac
    18301 gttccgctac cggaccggtt ggtttcgtcg tcagaccgta gaagcggtaa aacccttgag
    18361 ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt cgggtaaatc
    18421 cacactggca aaaatgctgg cgggaatgat agaacctacc agcggcgagt tattgattga
    18481 cgatcatccg ctgcattttg gcgattattc cttccgtagt cagcgtattc gcatgatttt
    18541 tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcacaaattc tggattttcc
    18601 actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta ttgaaacgat
    18661 gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt tggcacccgg
    18721 acaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag tcattattgc
    18781 cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta atctgatgct
    18841 ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata tcggaatgat
    18901 gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg agcgaggcag
    18961 caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga ttgccgggca
    19021 ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt ctgaatcctc
    19081 cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat gaggtgatgg
    19141 ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag gaacgaaagc
    19201 tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt caacatccgg
    19261 tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt gagctggatg
    19321 gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg cttgagtcgc
    19381 agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag gaggcggtgc
    19441 tggagattat tctgcaggaa ctgaaccgcc ggtcaccctc accctgaaag ggcgaggggg
    19501 ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga gaatattcgg
    19561 caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat gagcctgttt
    19621 gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg gagagggtta
    19681 gggtaagggg aaaagcgccc cccctgccgc agcctgctcc ggtcggacct ggcaactata
    19741 gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac
    19801 gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt
    19861 tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactgtc catcgcctac
    19921 ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac
    19981 aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag
    20041 tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg gaaagtttgg
    20101 ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt
    20161 gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat cagctcctac
    20221 agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg
    20281 accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca
    20341 aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg
    20401 cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcctccgg tatcaaagac
    20461 ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac cgttactatt
    20521 gaagatgtgg gtaactctgc ggcattcctg tgctccgatc tctctgccgg tatctccggt
    20581 gaagtagtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaactgaaa
    20641 taatcgttct gttggtaaag atgggcggcg ttctgccgcc cgcgttatct ctgttatacc
    20701 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    20761 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    20821 ctggtatcat gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga
    20881 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc
    20941 ccccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca
    21001 gcttaccgtg acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac
    21061 ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc
    21121 gctccccgga cctgattcct ccagggaaaa taatcatcgg ttggcacaaa tcacgcaata
    21181 tgcccgccaa ctggctagct cgcctgctat tatcgacaac cgatcgcgcc agcatctgaa
    21241 tgacgtcggt cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg
    21301 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt
    21361 acccgggctg gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg
    21421 ctggcgaagc agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc
    21481 agaactttgc caactggccg aaacacactc tctccaccct atttcacttt cccttctcga
    21541 aaagttgtta aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt
    21601 atgcgcgcgt ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga
    21661 atataaaaaa atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga
    21721 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
    21781 tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat
    21841 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    21901 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    21961 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    22021 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
    22081 ttggcttcct ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa
    22141 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    22201 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
    22261 aaaatgaccg tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc
    22321 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
    22381 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    22441 ttggcgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag
    22501 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
    22561 cctcgctgga ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt
    22621 tcgctaaggc gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg
    22681 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    22741 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    22801 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca
    22861 ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    22921 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa
    22981 ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata
    23041 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    23101 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    23161 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
    23221 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
    23281 cgcacggtct gcatgtcgat gccgaagaag tactcacgct ggacggtttc tgcaaactgc
    23341 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    23401 catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    23461 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    23521 cggttatcaa aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg
    23581 aacgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
    23641 tcctgaaaga taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc
    23701 gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt
    23761 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
    23821 aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca
    23881 tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct
    23941 catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac
    24001 tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa
    24061 acaatgcgtt tatccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc
    24121 ggactccgct gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt
    24181 gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt
    24241 gcgcttttct accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca
    24301 ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt
    24361 cgatgaaagc gatctttctg tgcatctggt aggacgaaaa atcaataaac gggaatgggc
    24421 tggcaacgcg tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct
    24481 ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg
    24541 ggggaatatc caacgcttca atcggttatg tgaagattac acagggttga aagaacacga
    24601 cgtcattggg caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag
    24661 gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc
    24721 aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg
    24781 caaaaacgag atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca
    24841 ggagcgactg cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc
    24901 aatgcaggag ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt
    24961 gtatcttgat ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga
    25021 ccagttatta cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt
    25081 ggcgcgtcca ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct
    25141 ggaagcaatg gcatcacgaa ttttgacccg cttacgcctc ccctttcgta ttggtttaat
    25201 tgaagtttat accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag
    25261 cgcgactatt attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg
    25321 acaattttgc gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga
    25381 taccaacttg cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg
    25441 gcgaagtgcg cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc
    25501 caccgttgga cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt
    25561 gggtgattct cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc
    25621 gagtggcggt aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga
    25681 aacaggttct ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga
    25741 gttgtctgat tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag
    25801 gtgcgcaagt gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc
    25861 gctttccgat cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac
    25921 ctgtctcgca gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg
    25981 tgatcgccga aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca
    26041 atgagcggca aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt
    26101 ataaacgcta tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg
    26161 tcattatata tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta
    26221 gcggcaattt ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg
    26281 aatttgatgc ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag
    26341 gcgattacca ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg
    26401 ttgctgaatc tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc
    26461 aggttttcct atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt
    26521 tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca
    26581 gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg
    26641 ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt
    26701 ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga
    26761 actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg
    26821 cagcagcaat gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat
    26881 cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct
    26941 cggtggcgtg taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat
    27001 ccagcaggta catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg
    27061 attcaccggt cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg
    27121 cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg
    27181 tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat
    27241 gcatcttgag cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc
    27301 tacggatgat ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta
    27361 agactattta cctgttatta acactctcaa gatataaaat tattatcagc gatataacag
    27421 gaagtaatta tcacgtgcgt g
//
LOCUS       DAJTRQ010000040        27126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000040 DAJTRQ010000000
VERSION     DAJTRQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..437
                     /locus_tag="OO332_001824"
     CDS             <1..437
                     /locus_tag="OO332_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR5942327.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OO332_001825"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OO332_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5942328.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="OO332_001826"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="OO332_001826"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5942329.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="OO332_001827"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="OO332_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5942330.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="OO332_001828"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="OO332_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5942331.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OO332_001829"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OO332_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5942332.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="OO332_001830"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="OO332_001830"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5942333.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="OO332_001831"
     CDS             complement(7412..8227)
                     /locus_tag="OO332_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5942334.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="OO332_001832"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="OO332_001832"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5942335.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="OO332_001833"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="OO332_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5942336.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="OO332_001834"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="OO332_001834"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5942337.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="OO332_001835"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="OO332_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5942338.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="OO332_001836"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="OO332_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5942339.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="OO332_001837"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="OO332_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5942340.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="OO332_001838"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="OO332_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5942341.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="OO332_001839"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="OO332_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5942342.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="OO332_001840"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="OO332_001840"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5942343.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OO332_001841"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OO332_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5942344.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="OO332_001842"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="OO332_001842"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5942345.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="OO332_001843"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="OO332_001843"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5942346.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OO332_001844"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OO332_001844"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5942347.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OO332_001845"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OO332_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5942348.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="OO332_001846"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="OO332_001846"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5942349.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="OO332_001847"
     CDS             24262..24876
                     /locus_tag="OO332_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5942350.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="OO332_001848"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="OO332_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5942351.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="OO332_001849"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="OO332_001849"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5942352.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6230 a         6903 c         7469 g         6524 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct aatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggtgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttctgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgtgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagaa aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatctccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgttcatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gctacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    27061 gaattccgac agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    27121 cacacc
//
LOCUS       DAJTRQ010000041        27051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000041 DAJTRQ010000000
VERSION     DAJTRQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..306)
                     /locus_tag="OO332_001850"
     CDS             complement(<1..306)
                     /locus_tag="OO332_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR5942353.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     "
     gene            complement(303..1565)
                     /locus_tag="OO332_001851"
                     /pseudo
     CDS             complement(303..1565)
                     /locus_tag="OO332_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1562..2470)
                     /locus_tag="OO332_001852"
     CDS             complement(1562..2470)
                     /locus_tag="OO332_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5942354.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2666..3433
                     /locus_tag="OO332_001853"
     CDS             2666..3433
                     /locus_tag="OO332_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5942355.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3484..4136)
                     /gene="pphB"
                     /locus_tag="OO332_001854"
                     /pseudo
     CDS             complement(3484..4136)
                     /gene="pphB"
                     /locus_tag="OO332_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4242..6803)
                     /gene="mutS"
                     /locus_tag="OO332_001855"
     CDS             complement(4242..6803)
                     /gene="mutS"
                     /locus_tag="OO332_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5942356.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            6879..7058
                     /locus_tag="OO332_001856"
     CDS             6879..7058
                     /locus_tag="OO332_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942357.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7090..7443
                     /locus_tag="OO332_001857"
     CDS             7090..7443
                     /locus_tag="OO332_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5942358.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7480..9558)
                     /gene="flhA"
                     /locus_tag="OO332_001858"
     CDS             complement(7480..9558)
                     /gene="flhA"
                     /locus_tag="OO332_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5942359.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9632..10642)
                     /gene="hypE"
                     /locus_tag="OO332_001859"
     CDS             complement(9632..10642)
                     /gene="hypE"
                     /locus_tag="OO332_001859"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5942360.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10639..11760)
                     /gene="hypD"
                     /locus_tag="OO332_001860"
     CDS             complement(10639..11760)
                     /gene="hypD"
                     /locus_tag="OO332_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5942361.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(11760..12032)
                     /gene="hypC"
                     /locus_tag="OO332_001861"
     CDS             complement(11760..12032)
                     /gene="hypC"
                     /locus_tag="OO332_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5942362.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12023..12895)
                     /gene="hypB"
                     /locus_tag="OO332_001862"
     CDS             complement(12023..12895)
                     /gene="hypB"
                     /locus_tag="OO332_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5942363.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(12899..13249)
                     /gene="hypA"
                     /locus_tag="OO332_001863"
     CDS             complement(12899..13249)
                     /gene="hypA"
                     /locus_tag="OO332_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5942364.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13461..13922
                     /gene="hycA"
                     /locus_tag="OO332_001864"
     CDS             13461..13922
                     /gene="hycA"
                     /locus_tag="OO332_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5942365.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14047..14658
                     /gene="hycB"
                     /locus_tag="OO332_001865"
     CDS             14047..14658
                     /gene="hycB"
                     /locus_tag="OO332_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5942366.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14655..16519
                     /gene="hycC"
                     /locus_tag="OO332_001866"
                     /pseudo
     CDS             14655..16519
                     /gene="hycC"
                     /locus_tag="OO332_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16522..17418
                     /gene="hycD"
                     /locus_tag="OO332_001867"
     CDS             16522..17418
                     /gene="hycD"
                     /locus_tag="OO332_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5942367.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17436..19121
                     /gene="hycE"
                     /locus_tag="OO332_001868"
     CDS             17436..19121
                     /gene="hycE"
                     /locus_tag="OO332_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5942368.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            19131..19673
                     /gene="hycF"
                     /locus_tag="OO332_001869"
     CDS             19131..19673
                     /gene="hycF"
                     /locus_tag="OO332_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5942369.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19673..20440
                     /gene="hycG"
                     /locus_tag="OO332_001870"
     CDS             19673..20440
                     /gene="hycG"
                     /locus_tag="OO332_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5942370.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20437..20847
                     /gene="hycH"
                     /locus_tag="OO332_001871"
     CDS             20437..20847
                     /gene="hycH"
                     /locus_tag="OO332_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5942371.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            20840..21310
                     /gene="hycI"
                     /locus_tag="OO332_001872"
     CDS             20840..21310
                     /gene="hycI"
                     /locus_tag="OO332_001872"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5942372.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21380..22312
                     /locus_tag="OO332_001873"
     CDS             21380..22312
                     /locus_tag="OO332_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942373.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            22296..22607
                     /locus_tag="OO332_001874"
     CDS             22296..22607
                     /locus_tag="OO332_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR5942374.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22604..23023
                     /locus_tag="OO332_001875"
     CDS             22604..23023
                     /locus_tag="OO332_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5942375.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(23138..24562)
                     /gene="ascB"
                     /locus_tag="OO332_001876"
     CDS             complement(23138..24562)
                     /gene="ascB"
                     /locus_tag="OO332_001876"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5942376.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24571..26019)
                     /gene="ascF"
                     /locus_tag="OO332_001877"
     CDS             complement(24571..26019)
                     /gene="ascF"
                     /locus_tag="OO332_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR5942377.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            26279..>27051
                     /gene="ascG"
                     /locus_tag="OO332_001878"
     CDS             26279..>27051
                     /gene="ascG"
                     /locus_tag="OO332_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5942378.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKA"
BASE COUNT         6193 a         7156 c         7298 g         6404 t
ORIGIN      
        1 tgtgctgtgc aaatgcacca ccgctttaca gcgcggattg ttgcgataca gcgccagatg
       61 aaagagcacc tctttcgagg gtttgtcacc acttaaccat tcgccatccg cggcgacttt
      121 ggaaagccgc tgcggatcga gattgcccag gcatgaacct gtcggtgtcg ccagtaaatt
      181 cccgtcaggt aaaagcagcg acagattgcc agccgaaccg gttgcatagc cgcgctgaaa
      241 gaatgaactg gcaatccgcg tcatctcctc tcgcaaagac tgctctactt ttgcgaaatc
      301 gctcatgata aaaactctct ttgggctcgt gaaaaaaagg cttcatcacc gaagttgcca
      361 gatttaaggg cgagtgagac aggcttatcc agtgcgttta cccacggcac gccgggggaa
      421 atggttgggc caatatgaaa cccttttatg cccaggctct gtgtgactac gccggaggtc
      481 tcaccacctg cgacaataaa gcgtgtcatg ccttccgctg ctaaccgcgc cgctagttga
      541 gaaaacagag tttctactgc ctgactggct ttttgtgcac cgtattgctg ttgaattgct
      601 gccaatgcgt cagtgctggc ggtggcaaaa accagtggag caagtacact ttcctggccc
      661 agaacccact ctgccagttc gtgagcataa gcggccagag tttcggttga gaggcagcgt
      721 gccacatcaa cttcacgggc tggtgcaatt tgacggtaat gtgccaccta gcggttggtc
      781 atttgagagc atgaaccgga gagcactacg ccgcgcccag cgagcggacg ccctgcttcg
      841 cgagcctggt taccgttttc ttgcgcccac tgccgggcca ggccaatcgc cagaccagaa
      901 ccgcccgtta ccagtggggc atcgcgcaag gcttctccct gaatttccag atggtgttcg
      961 gtcagcgcat caagcaccgc gtagcggtag ccctcttgct gtaagcgagc cagctcttga
     1021 cgaacggcat ccacaccttg ttcgaaaaca tgtgccgaaa cgacgccgca gcgccctgtg
     1081 gattgcgctt caaccagacg gggaagatag ctgttggtca tgggatttac cgggtgatgg
     1141 cgcatcccgg attcggccag cagttgattc attacgaaca aatacccctg ataaaccgta
     1201 cgtccgttga ccggcagggc cggagagaag accgtaaacg gcgtgtcgag agcatccatt
     1261 aatgcatcgg taaccgggcc aatattacct ttcgccgtac tgtcgaaagt agagctgtat
     1321 ttgaaataga tctgtttgca accttgctgt tgcaaccagc tcagagccgc cagcgattgc
     1381 tgtgtggctt caaccaccgg acaggagcgc gttttcaggc tgatcaccag tgcgtcgatt
     1441 gcttccggca ttttacctgt tggaacaccg ttaatttgta ccgttggtag accgttttcc
     1501 accagaaaac tggcgatatc cgtcgcgccg gtaaaatcat cggcgataac gccaatcttg
     1561 atcatgattt cgctcccggt agagtgatgc cagagaaaat cttgataact gcgctatcgt
     1621 cttctttccc gtaacccgcg ttactggcgc tggtgaacat attcaatgct gttgaggcca
     1681 atggcagcgg gaagtgcagg gctttggctg tatcggcaac cagaccaaga tccttaacaa
     1741 aaatatcgac ggctgaatgc ggggtgtaat cgccatccac cacatgacgc atccggtttt
     1801 cgaacatcca ggaatttccg gcggcattgg tcacgacgtc atacatcaca tccagcggga
     1861 tccccgcacg ggctgcaagt gccatcgctt cggctccggc agcaatatgt acgcccgcta
     1921 acaactggtg aataattttt acggtcgaac ctagtcccgg ttctgcacct atgcgataaa
     1981 cttttccggc aacggcttcc agcacgggtg ccagtcgttc aaaggcaata tcgctaccgg
     2041 aggccatgac agtcatttca ccgttagcgg cttttactgc accaccagaa actggcgcat
     2101 ccagcatttc cagatcgaat ccagccagag cggtagcaat ttcttgcgca tcagcactag
     2161 cgatagtgga agaaaccatt actgccgtac cgggtttcag atgttgtgca acgcctgttt
     2221 caccaaacag cacctgttta acctgggccg cattgaccac cagcaccagc agagcgtcca
     2281 gtttttcggc aaacgtcgcg gcgttatcag aaaccccgca agcacctgcc tctttcaacg
     2341 tagcgcaggc attgctgttc aggtctgcgc cccaggtaga aaaacctgcg cggacacatg
     2401 acagtgctgc tcccattccc attgacccta agccaacgat accgacatga aactcagatc
     2461 ccgttttcat ctgctctcct tgttaattta agtgatattt tgtttgacat tgtgaatata
     2521 agcgctggga gataacgata tggtgagctg attcacataa attaacattt tgtgttattt
     2581 tatgtgaact aagcgttagt tgcgccgcgc acgtttcgca ggcaaatagc gtagaatgtc
     2641 agcaggacaa aggaaggagc aaaagttgat acccgtagag cgtcgccaaa tcatccttga
     2701 gatggtagct gaaaaaggca ttgtcagtat tgctgaacta acggacagaa ttaatgtgtc
     2761 acatatgacc attcgtcggg atttacaaaa actggagcag cagggagccg ttgtgctggt
     2821 gtccggaggc gtccagtctc cgggacgcgt ggcgcatgaa ccttctcatc aggtaaaaac
     2881 tgcgctggca atgacgcaaa aagcggctat tggcaagctg gcggcaagtc ttgttcagcc
     2941 gggaagttgt atctatctgg atgcgggaac gaccacgtta gcgatagcac agcatctgat
     3001 tcacatggag tcactgactg tggtcacaaa cgatttcgtt attgcgaact acttgctcga
     3061 caacagtaat tgcacaatta ttcacactgg cggtgcagtg tgtcgggaaa accgttcctg
     3121 tgtcggggaa gccgctgcga ccatgctgcg cagcctgatg attgatcagg cttttatttc
     3181 tgcatcgtca tggagtgtgc gggggatttc tacgccagcg gaagataaag tcacggtgaa
     3241 acgggcgatt gccagtgcta gccgccagcg agttttggtc tgtgatgcga cgaaatatgg
     3301 tcaggtggcg acatggctgg cgttaccatt aagcgagttt gatcagatta ttacagacga
     3361 cggtttgccg gagagtgcca gtcgcgcgct ggcgaagcag gatctctctt tgctggtagc
     3421 gaaaaatgaa taatggcctg caataacatc tggttactca tgcttcacag aagaagcatg
     3481 agactacctt attttataaa atgacagccg cccacttttc ggcgatccgg tatcaatata
     3541 aatctggtta gcgaacgtct gaatgttatc aaacatcata tgtccaaata taaaataatc
     3601 agcgccgttt atttgttgta actcgccatt aagcgatttc tgcacacgat aaacaggcca
     3661 gagtaattcg ctctccgcta tttctttgtc aaagagatat tcatcacccg gataatctgc
     3721 atgtgcgatg acatatttta ttatgtcgtt agtgatttca ataatatgtg gaaggtgatg
     3781 gaatttcagc aacagatcta ttgcctcttg ttgcaatcat ttaaatcgaa aaaccagtca
     3841 ccaccgctgg caagccacat attgccatcg ccggtcgcga acgcatccag cgccattgct
     3901 tcgtggttgc ctttaacgga gataaaccag ggctggttta gcaggcgcag gacgtcaaga
     3961 ctctccggcc cacgatcaat attatcgccg acagaaataa gtaagtcggt ttcggggaaa
     4021 aaagagagtt gatgtaagcg ggattgtaat aactgatatt caccatgaat atcaccaacg
     4081 acccatatat ggcgatagtg atgggcattg attttttgat agcgtgtaga tggcatggtt
     4141 ttaccctgta aaataagctt tcctattata cagggtattt ttatttgatt cgtcagttgt
     4201 cgttaatatt cccgatagca aaagactatc gggaattgtt attacaccag actcttcaag
     4261 cgataaatcc actccagcgc ctgacgcgga gtcagtgaat ccgggtcgag attttccagt
     4321 gcttcgaccg caggcgaagt ttcttctggc actgacagca aagacatctg cgtaccatcc
     4381 acttgcgtcg cggcggcgtt cggcgaaatg ctttccagct cacgcagttt ttgccgtgcg
     4441 cgcttaataa cctctttcgg tacgcccgcc agagcagcaa ccgccagacc gtagcttttg
     4501 ctcgccgcgc cgtcctgcac gctgtgcata aaggcgatag tgtcgccgtg ctccagcgca
     4561 tcgagatgta cgttggcgac gccttccatt ttctccggta gctgggtcag ctcgaaatag
     4621 tgggtggcaa acagcgtcaa tgccttaatc ttattcgcca gattttctgc gcacgcccac
     4681 gccagcgaca gaccatcgta ggtggacgtt ccacgcccga tctcatccat taacaccaga
     4741 ctgtattcgg tggcgttatg taaaatattg gcggtttcag tcatctccac cataaaggtt
     4801 gagcgcccgg aagccaggtc atctgccgcg cctacgcggg taaagatgcg atcgataggt
     4861 ccaatctcga ctttttgtgc cggtacatag ctgccgatgt aggccatcag cgcaatcagt
     4921 gcggtctggc gcatataggt acttttaccg cccatgttcg ggccggtaat aatcaacata
     4981 cggcgctgcg gcgacagatt cagcgggtta gcgataaatg gctcattcag tacttgttca
     5041 actaccggat ggcgaccttc ggtaatgcga atgcccggtt tatcaatgaa ggtcgggcag
     5101 gtgtagttca gggtataggc ccgttccgcc aggttaacca gcacgtcgag ttccgccagc
     5161 gcgctcgcgc tctgttgcaa cgcttccaga tgcggcaaca gcaggtcgaa cagctcttca
     5221 taaagctgtt tttccagtgc cagtgctttg ccttttgagg tgagaacttt atcttcgtac
     5281 tcttttagct ctggaatgat gtagcgctcg gcgtttttca gcgtctggcg acgcatgtag
     5341 ttgatgggtg ccagatggct ttgcccacgg ctgatttgaa tgtagtagcc gtgcaccgca
     5401 ttaaagccaa cttttagcgt gtccaggccg gtacgttcac gctcgcggac ttccagacgc
     5461 tccagataat cggtcgcgcc gtcagccagc gcgcgccact catccagctc ttcgttatag
     5521 cccgatgcga taacaccacc gtcgcgtaac agcaccggcg gtgtgtcgat gattgctcgc
     5581 tccagcagat cgcgcagctc ggcaaactct cccatcttct cacgtagcgc ctgtaccggt
     5641 gcactatcga cagtttctaa ctgcgcacgc agctccggca gttgctggaa agcgtggcgc
     5701 atacgggcca gatcgcgtgg gcgagcagtt cgtaaagcca gacgtgccag aatacgttcc
     5761 aggtcgccga cctgacgcag taccggctgc aactcggcgg tgaaatcctg caatgcgcca
     5821 atagtttgct ggcgctcaag caacacgcga gtatcgcgca ctggcatatg cagccagcgt
     5881 ttcagcatac gactacccat cggcgtgacg gtgcagtcga gcacagaagc cagcgtattt
     5941 tccgcaccgc ccgccaggtt ctgagtaatt tccaggttac gacgtgtcgc ggcatccata
     6001 atgatgctgt cctgctcacg ttccatggtg atagaacgaa tatgcggcag ggtcgtgcgt
     6061 tgggtatctt tcgcatactg caacagacaa ccggcagcac aaagtccgcg cggcgcgttc
     6121 tcgacgccaa aaccgaccag atcgcgggtg ccaaattgca gattcaactg ctggcgcgcg
     6181 gtgtcgattt caaactccca cagcgggcga cggcgcaggc cgcgacggcc ttcaatcagc
     6241 gacatctcgg cgaaatcttc tgcatacagc agttccgccg gattagtgcg ttgcagctct
     6301 gccgccatcg tttcgcggtc ggccggttcg ctcaggcgaa aacgcccgga gctgatatcc
     6361 agcgtcgcgt agccgaaacc tttgctgtcc tgccagatag ccgccagcag gttgtcctga
     6421 cgctcctgta acagggcttc atcgctgatg gtgcctggcg taacgatacg cacaactttg
     6481 cgctcaaccg gacctttgct ggtcgccgga tcgccaattt gttcgcagat ggcaacggac
     6541 tctccctgat tcaccagttt ggcgagatag ttttccaccg catggtaggg aatccccgcc
     6601 atcgggatcg gctctcccgc cgaagcaccg cgtttggtca gtgaaatatc cagcagttgc
     6661 gacgcgcgtt ttgcgtcgtc ataaaacagt tcataaaaat cacccatccg gtaaaacagc
     6721 aggatctcgg gatgctgggc tttcagcttg agatactgct gcatcatggg cgtatgggcg
     6781 tcgaaatttt ctattgcact catggggtta tgtccggttc cctgatatta aatggggtgt
     6841 gatggttttt attttcgttg taatcacata aggcgcacat gataccggag ttaatcattt
     6901 caggggaaga agcgaacggc gagttcttga tgctggttcc gggaaagatg ctgttttgca
     6961 gcttttgcgt gatgcttatg aagcaagtac gcaaaattgt atcgattggc gaacccttct
     7021 tctttagagt catagtgccc tttaagatta agatgtaata taaatgcatc ttaatgaggt
     7081 ggaggcagca tgtccggcaa gcgtatctct cgtgaaaaac tgacgattaa aaaaatgatc
     7141 gatctttatc aagcgaaatg cccgcaggcg tcagcggagc cggagcatta cgaggcgttg
     7201 tttgtttacg cgcaaaagcg gctggataaa tgtgtgttcg gcgaagagaa accagcctgt
     7261 aagcagtgtc cggtgcactg ttaccagcct gcaaagcgtg aagagatgaa acagattatg
     7321 cgttgggccg ggccgagaat gctgtggcgt catccgatct taaccgtgcg tcatctcatc
     7381 gatgacaagc gtccggtgcc agaactgccg gaaaaatatc gtccgaaaaa acctcgtgaa
     7441 tagtatgccg gacaggcaga tctgtccggc aatttgcagt taaatcaatg ccgatttatc
     7501 aattcccagc cgcttcatcc gtgacagcag ggtcgtgcgt ttcagcccca gacgttgcgc
     7561 agcgccttta ggcccggcaa ccacgctgtt ggtttctttc agcacgcgca caatcaactg
     7621 atattcatct tcgccctcct gggcgacaac cgttgcggca ggcggcgttt caggctccgg
     7681 taaagcaata tctggcaatg acagctgcag cacgttgccg cgtgttagca atactgcgcg
     7741 ctcaatgacg ttttccagtt cgcgtacgtt acccggccac tccatgttgc tcaaggtgcg
     7801 cagcgtctcg gcaggaatgc tgtcgatatt gcgccccaga cgacgggcaa ttttgaaggt
     7861 aaaggctttc gccagcagcg gaatatcttc cggacgctcg cgtagtggcg gcaggtgaat
     7921 cgggaatacg ttcaggcggt aatagagatc gctacggaac tctcggtcgg cgaccatttt
     7981 tttcagatcg cggttagtcg cggcaattaa acgtacgtcc gtctgaatga ttttgttgct
     8041 gccgagacgt tcaaactcct gttcctgcaa tacacgcagc aacttcggct gtaactccag
     8101 tggcatatcg cccacttcgt cgaggaacag agagctttta tccgccagtt caaaacgacc
     8161 aatgcgctgg gcgctggcac cggtaaaagc cccacgctca tgaccaaaca gatcgctttc
     8221 cagcaatccg gcaggcatcg ccgcgcagtt cattttgacc atgcggcgat tattacgccc
     8281 actgagatta tggatcgcac gggcaatcag ctctttaccc gtgccagttt caccgaggat
     8341 cagcacggta ctgtcacttt gcgccaccat ttcaacttgt ttaagcacgc tgtacatggc
     8401 ttcgctgcgg ccaataatct cgccaaattc actatcaaca ttgttgagtt gctcggtcag
     8461 ggcgaggttt tcatcaacca gccgttcttt cagacgatgg atttcctgat aggcgagggc
     8521 gttatcgaca gcgattgcca cacgttcggc aatctggcgc agcaaattca gattggtagt
     8581 ggtaaacact ttctcttcgc attgcgccag tttcagcacg cccagcatgg tgtcgccaga
     8641 catcagcggt aacaggcaca aggtttgaat ctggttgccc caggtgtcga acaacatgcg
     8701 ttcatagggg gctaaatcgt cccgctcgta gagattgatc agcagcatct ctttactttt
     8761 gaacacgcgt tcggtgaggg ttccggcttc atcgacttcg ctctgttcgt gggcgggatg
     8821 ttgtttatca agatagtgag tggagtagat gttgagtttg tttttacggt ggctgcgtaa
     8881 gacaatgctg atatcgtcaa tgtcgaaata gtaatggatt tctttggcga cttcgctgac
     8941 cagttcgtcc atatccaggc gggaaagcac cgcgttggta atggcaacca ggatgcggaa
     9001 gttatcgcgc tcccggcata acaactcata gtcgacattg ttgacgacgc ggctctggat
     9061 ttgttcggtg acgacagaaa cgatctgcgt aaatgtttgc agacgattga actctttttc
     9121 gctccaggga cgatcgtcat aacgaataaa ttcacagcca ccaaaaatat gccctcccgc
     9181 cgccagtggc atcaggcaat agtgaccaaa tttggggtat agcccacctg cggccagctg
     9241 cggccaggtt tcacaaaatt cttcgtaact gcaatgcagc gtatcagggc gcgacaaaat
     9301 gctgcgtacc ggaccgtgtg ccagaacagt ttcgtcttca tatttaatgg gggtgtcttt
     9361 ttcacgcgac gcgtaataag acgcacgttg agtctgcgct tgccacaaca caatagccgc
     9421 gttgtcggcg agcgcagaac gctttaccag ttgcgaaaga gcctcacaca gcgaggccag
     9481 atcgggctgc tgcaatagtg tccgagtgat gtcgaacaac ccttgctgtc cgagatcact
     9541 catcggtgta tatgacattt tgttcttcca ggaaagcacc gcaaagcggt gcaaaaggtc
     9601 attgactaac tataggagat ttagaatttt attagcatat acgcggaagc ggttcggcgt
     9661 gtggtaaatc gagggttcgt ttcacgccat acagaccggc aagacgaaca cctttacgtt
     9721 ccaccacttc accaatcagc gccgcgtctt tccccagtgg atgggaatgt aacgctgcca
     9781 gcacttgctc tgccgcgttg cgttcaacgg cgatcaccag tttaccttcg ttggcaaagt
     9841 tcagggcgtc cagtcccagc aattcgcaaa cgccgcgcac ggcaggttta accggcagcg
     9901 ctgattcaga aatttcaata ccacaaccgc aggctgccgc gaactcatga accaccgcgt
     9961 ttacaccacc acgggtggca tcacgcagcg ctttcacgcc gggaatgtca cgcagcgtct
    10021 gaataagcgg cgtcagcacc gcgcagtcgc tgaccagttc gccatccagc cccagctgct
    10081 cacgcaggtt aaggatagtc gccccgtggt cgccgagtgt accgctaacc agcaatacat
    10141 cgcctgcggt tagcgtctgt gcgccccagt gaatattcgc cggaattgcg cccatgccag
    10201 cggtgttgat aaacagttta tctgccgcgc cgcgctgcac cactttagta tcgccagtaa
    10261 cgatggcaat gcctgccgtg cgggcggttt ctgccatgct ggtcactgcg gctttcagtg
    10321 tctccatcgg caatccttct tcgaggataa agccacagga gagatagcgc ggaatagcgc
    10381 cactgaccgc aacgtcattc gcggtgccgc aaatcgccag cttgccgata ttaccgccag
    10441 gaaagaacag cgggtcaatg acgtaactgt cggtggagaa cgccagacgg tcgccttccg
    10501 ctaccagctg cgccagatca agacgtgcct gatcttcctg ctctgccagc cacgggttgg
    10561 caaaggcttc cataaacagg ctgttgatta attgctgcat cgcctggccg ccgctgccgt
    10621 gggcgagttg gatattattc acgcttcact ctcctgctga cgatactgat accacgcggc
    10681 gcacgctcct tcggaggaga ccatcagtgc accaaatgcg gtttgaggat tacaggtgtt
    10741 accaaacagc gggcattgat gcggcttaca tttgcccgtt aatacctcac cacaacgcgc
    10801 gcgcgggtca tcgcagacct gctgcggtgc cgggcggaaa tgtgcttcgg catcgaatcg
    10861 ttgataatcc ggcgtcaggt gcacgccaga agattcaatc acgcctaagc cgcgccattc
    10921 gctgtcgccg ttgacacaga acacatcggc aatcgcctgt tgcgccagca ggttaccggc
    10981 atccggtacc acccgacgat actgattctc taccttgctg tgggccgcta ttttctgctc
    11041 caccagcatg accacgcctt gcagtagatc aaggggttcg aaaccagcga ccaccagcgg
    11101 acgatgaaaa tcgctggcga taaaattata ggcatcagtg ccgataacca tactaacgtg
    11161 gcctggcgcg aggaacgcgt cgataccgtt atccggctgt tccagcaaac tgcgcagcgt
    11221 cgggataagc gtaatgtgct ggcagaagaa gtaaaaattc tgcacatcac gcgctttcgc
    11281 ctgttgcaga gtgatggcgg tggtcggcat ggtggtttca aaacctaagc cgaagaacac
    11341 cactttgcgg gttggattct cctgcgccag tttcaacgca tccatcggcg agtaaacgat
    11401 gcgcacatcg gcaccgcgtg cttttgcctg caacagcgat ccctgtttcc ctggtacgcg
    11461 catggcgtcg ccaaaggtac agaagatgac ttccggatgg ctggcaatct ccacgcaggt
    11521 gtcgattcta cccatcggca gtacgcacac cgggcaaccc ggaccgtgga taaactcaac
    11581 gttttcaggc agtaactggt cgaggccgaa tttaaaaatg gcgtgggtat gaccgccaca
    11641 cacttccata atccgcagag ggcgttcggc ggtgtaagag agatgtgaag cacgttcgcg
    11701 cagatgctca attaactgca tcacctgttc cggcgcgcga tattcatcaa caaaacgcat
    11761 tatttttcct cgccatacaa cagcgcaccg acatccggct caacgtcaaa catgttttgt
    11821 aaggcgtcga gagtgtcgcg tgcttcggct tcattaatta cgctcatggc aaagccaacg
    11881 tgaaccagta cccactggcc cacgcgcggc tgaccgtttt catcgcagct gccgactaac
    11941 gttaaatcga catcgcgctg aatgccgcag acgtcgactt tcgcctggtt gccgtcaatg
    12001 gtgcggatct ggccgggaac gcctatgcac atcgctgtgt ctccagccag ttcagccact
    12061 ggtccatccc ttcgccgctg gtggcggaaa taaggatgat ttcaatttct ggattgactt
    12121 cgcgggcgca ggcgatgcac ttctcaacgt caaagttgag atacggcaac aggtcaactt
    12181 tgttgagcag catcagcgag gcggcggcaa acatatgcgg atatttcagc ggtttgtctt
    12241 caccttcggt aacggaaagc accgctactt tgtgtttttc accgagatcg aagctggccg
    12301 ggcatacgag gttgccaacg ttttcgataa acagaatacc gttatcgtcc agtggcagac
    12361 gcggtgcggc gtcggcaatc atctgtgcgt caagatggca gcctttaccg gtgttcacct
    12421 gaatcgctgg tgtaccggta gcgcgaatgc gtgcggcatc gttcacggtt tgctggtcgc
    12481 cttcaataac cgcgcacgga acgctgtctt tcaggcgcat taaggtttcc gtcagcaggg
    12541 tggttttacc ggaaccaggg ctggaaacca ggttgagcac cagttgcttg cgggcagcaa
    12601 agcgcgcgcg gttgcgttca gccagacggt tgtttttgtc cagcacgtca atttcgactt
    12661 ccagcatccg acgctggctc atgcccggtg tgtgagtgcc cgcttcgcca tgaccgtaat
    12721 gcaggtcgcc ttcttcagtc tggctggggg taaattcagg cgctttaatg ccggtgattt
    12781 tcatctgcgg gcgtgccgcc ggggcaaatg gcgcgctacg aaacgcggaa tgagggttat
    12841 gttcatcacc ctcgatatac aggttgcctt caccgcaacc gcatgttgta cacatcgctc
    12901 actcctggtc tatttctatc cgccgaatct gtaaaccgtc gtctgccaca atctgtagca
    12961 tgtcaccatg acactgtgga cagcggcgga cgcgctgggt cagtagcgtc acatactgtt
    13021 ggcatgtttc acaccagcat tcggcctctt gttcttcgag gtgcagttta caaccttccg
    13081 ccacgctgcc gcggcaaacc agatcaaaac aaaaggcaag agagctggtt tcgacacaag
    13141 aaaatgcgcc aattttgagc cagaccccag ttacgcgttt tgcgccgtgt tttgcggcct
    13201 gctgttcgat caattccagt gcccgttggc agagggttat ttcgtgcata tcgcctccca
    13261 ttaactattg ccagctacaa gcaataattg cgccagtgtt gattatccct gcggtgaata
    13321 atgtcgatga tgtcgaaatg acacgtcgac acggcgacga aattcatctt tagcttaaaa
    13381 atctctttaa taacaataaa ttaaaagttg gtacaaaaaa tgcttaaagc tggcatctct
    13441 gttaaacgag taacctgaca atgactattt gggaaataag cgagaaagcc gattacatcg
    13501 cacagcggca tcgtcgccta caggaccagt ggcacatcta ctgcaattcg ctggttcagg
    13561 ggatcacgtt atcgaaagcg cgcctgcatc acgccatgag ctgcgcgccg gacaaagaac
    13621 tctgtttcgt cctttttgaa cattttcgta tttacgtcac cctggcggat ggctttaaca
    13681 gccacaccat cgagtattac gtcgaaacaa aagatggcga agacaaacag cggattgcgc
    13741 aggcgcaact gagcattgac ggcatgattg atggcaaggt caacatccgc gatcgcgaac
    13801 aggttctgga acactatctc gaaaaaatcg ctggcgttta cgacagctta tacaccgcca
    13861 ttgaaaacaa tgtgccggtg aatttaagcc aactggtaaa gggacaaagc ccggcagcat
    13921 gagctgaggc tttgcccgtt ttgcaggcgt tacgcctgtt tggggatggg cgtgtcgatg
    13981 agtgtcgaaa atgacatttc atcggcatgt tttcgtcaaa aatgacaatc acctgaggaa
    14041 tgcctggtga atcgttttgt aattgccgac tccacgctct gtatcggctg ccacacctgt
    14101 gaggccgcct gttcagagac gcatcgccag cacggcctgc aatcaatgcc gcgcctgaga
    14161 gtgatgctca atgaaaaaga atctgcgccg cagctctgtc accactgtga agatgcaccc
    14221 tgcgcggtgg tctgcccggt taacgccatc acccgcgttg atggggccgt gcagttgaat
    14281 gaaagtctgt gcgtaagctg caaactgtgc ggcatcgcct gcccgtttgg cgcaattgaa
    14341 ttttccggca gccgtccgct ggatattccg gcaaacgcca ataccccgaa agcgccaccg
    14401 gcaccgcctg ctccggcgtg tgtcagcaca ttgcttgact gggtgccagg tattcgcgcg
    14461 atcgccgtca aatgtgacct ttgtagcttt gatgaacaag gtccggcctg tgtgcggatg
    14521 tgcccgacta aagccctgca tctggtggat aacaccgata tcgcccgcgt cagcaaacgt
    14581 aagcgtgagc tgacttttaa cacggacttt ggcgatctca ccttgtttca gcaggctcaa
    14641 agtggagagg ctaaatgagc gcaatttccc tgatcaatag cggcgtggca tggtttgtcg
    14701 ccgccgctgt tctggcattt ctcttttctt ttcaaaaagc gctaagtggc tggatagccg
    14761 gaattggcgg cgcggttggt agtctgtata cggcagccgc gggcttcact gtactgactg
    14821 gctcggttgg cgtgagcggt gcgctgtcgc tggtaagcta cgatgtgcaa atctccccgc
    14881 ttaacgcgat ttggctgatt accctcggtc tttgcggtct gtttgtcagc ctctataaca
    14941 ttgactggca tcgccacgcg caggtgaagt gcaacggctt gcagatcaat atgttgatgg
    15001 ctgccgccgt ctgcgccgtc atcgccagca acctcggcat gttcgtggta atggccgaag
    15061 tcatggcctt gtgtgcggtg ttcctcacca gcaacagcaa agagggcaaa ctgtggtttg
    15121 cgctggggcg tcttggcact ctgctgctgg tgattgcttg ctggctgctg tggcagcgtt
    15181 atggcacgct ggatctgcgc ctgctggata tgcgtatgca acagctgccg ctcggttccg
    15241 atatctggct gctcggttcc gatatctggc tgctcggtgt gattggcttt ggcctgctgg
    15301 ctgggattat tccgctgcac ggctgggtgc cgcaggcaca tgctaacgcc tctgcgccag
    15361 ctgccgcgtt gttttctacg gtagtcatga aaattggcct gctgggcatg ttaaccctgt
    15421 cactgctggg cggtaatgca ccgctgtggt gggggatcgc gctgctggtg ctcggcatga
    15481 tcaccgcgtt cgtcggtggt ctgtatgcgt tgatggaaca caacatccag cgcctgctgg
    15541 cttaccacac cctggaaaat atcggcatta tcctgctggg gctggggctg gggctggggc
    15601 tggggctggc gtaacgggta tcgcgctcga acaaccggcg ctgattgctc ttggcctggt
    15661 cggtggtctg taccatctgc ttaaccatag cctgttcaaa agcgtactgt tcctcggggc
    15721 ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa aaactcggtg gtattggcaa
    15781 gaaaatgccg gttatctcca tcgccatgtt agtcgggttg atggcaatgg ctgcgctgcc
    15841 gccactgaac gggtttgccg gggaatgggt tatctatcaa tccttcttca aactgagcaa
    15901 tagcggcgcg tttgttgccc gtctgctggg gccgctgctc gctgtgggtc tggcaattac
    15961 cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc gtcacgttcc tcggcgcgcc
    16021 gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc ctgatgagcg taagcgtagt
    16081 ggcactggcg atttgctgcg taattggcgg tgttgctgcg ccgtggctgc tgccgatgct
    16141 ctctgctgct gtacctctgc ctctggagcc tgctaacacc accgtttctc aaccaatgat
    16201 cacattgctg ctgattgcct gcccgctgct gccattcatc attatggcga tttgcaaagg
    16261 cgaccgcctg ccgtcacgtt cccgcggtgc ggcctgggtg tgcggctacg accatgaaaa
    16321 atcaatggtg attaccgctc acggttttgc catgccggtt aaacaggcgt ttgcgccagt
    16381 gctgaaactg cgcaaatggc tgaatccggt gtcgctggtg ccgggctggc agtgcgaagg
    16441 tagtgcgttg ctgttccgcc ggatggcgct ggttgaactg gcggtactgg tggtgattat
    16501 tgtttcacga ggagcctgag aatgagtgtt ttatatccgt taattcaggc gctggtgtta
    16561 tttgccgttg cgccgctgct ctccggtatt acccgcgtgg cgcgcgcccg tctgcataac
    16621 cgtcgcgggc cgggcgtgtt gcaggagtat cgcgacatta tcaaactgct gggccgccag
    16681 agcgttggcc cggatgcctc tggctggatg ttccgcctga cgccgtatgt gatggtgggc
    16741 gtcatgctga ctatcggttc tccgctgccg caactgggcg atttgatcac cttactgtat
    16801 ctcttcgcca tcgcgcgttt cttctttgcc atttccggtc tggataccgg tagcccgttt
    16861 accgctatcg gtgcgagccg tgaagcgatg cttggcgtgc tggtcgaacc gatgttgctg
    16921 cttggtctgt gggttgccgc acaggttgcc ggttccacca acatcagcaa cattaccgac
    16981 accgtttatc actggccgct gagccaaagc atcccgctgg tgctggcgct ctgcgcctgt
    17041 gcgttcgcca cttttatcga aatgggcaaa ctgccgttcg acctggcgga agccgagcag
    17101 gagttgcagg aaggtccgct ctccgaatac agcggtagcg gctttggcgt catgaaatgg
    17161 ggtatcagcc tgaaacagct ggtggtgttg cagatgttcg tcggggtgtt tattccgtgg
    17221 ggacaaatgg aaaccttcac cgtcggtgga ctgctgctgg cgctggtgat tgccatcgtc
    17281 aaactggtgg tcggcgtcct ggttatcgcg ctgttcgaaa acagtatggc ccgtctgcgt
    17341 cttgatatta ctccgcgcat tacctgggct gggtttggct ttgcattttt agcgttcgtc
    17401 tccttgctgg cggcgtgatt aaagagagtt tgagcatgtc tgaagaaaaa ttaggtcaac
    17461 attatctcgc cgcgctgaat gaggcatttc caggcgtcgt gctggaccac gcctggcaga
    17521 ccaaagatca gctgactgtc accgtaaagg tgaactatct gccggaagtg gtggagtttc
    17581 tctactacaa gcagggtggc tggctgtcgg tgctgtttgg taacgacgaa cgcaaactga
    17641 atggtcatta cgccgtttat tacgtgctgt cgatggagaa gggcaccaag tgttgggtaa
    17701 cggttcgcgt cgaagttgac gccaacaaac cggagtatcc gtccgtgacg ccgcgcgttc
    17761 cggcggcggt gtggggcgag cgcgaagtgc gcgatatgta cggtttgatt ccggttggtc
    17821 tgccggatga ctggccggat gaactttatc cgctgcgtaa agacagcatg gattatcgtc
    17881 agcgtccggc accgactacc gatgctgaaa cctacgagtt catcaacgaa ctgggcgaca
    17941 agaaaaacaa cgtcgtgccg attggtccgc tgcacgtcac ttctgacgaa ccgggtcact
    18001 tccgtctgtt cgtcgatggc gaaaacatta tcgacgccga ctaccgtctg ttctacgtcc
    18061 atcgcggtat ggaaaaactg gcggaaaccc gcatgggtta taacgaagtg accttcctct
    18121 ctgaccgtgt gtgcgggatc tgcggctttg cccacagcac cgcctacacc acgtcggtgg
    18181 aaaacgcgat gggtattcag gtgccagaac gcgcgcagat gatccgcgcc attctgctgg
    18241 aggtggaacg cctgcactcg catctgctca acctcggcct cgcctgtcac tttaccgcct
    18301 tcgactccgg ctttatgcag ttcttccgcg tgcgtgaaac ctccatgaaa atggcagaga
    18361 tccttaccgg tgcgcgtaaa acctacggcc tgaacctgat cggcgggatt cgtcacgatc
    18421 tgctgaaaga tgacatgatc cagacccgcc agctggcgca acaaatgcgt cgtgaagtgc
    18481 aggagctggt ggatgtgctg ctgagtacgc cgaacatgga acaacgcacc gtcggcattg
    18541 gtcgtctgga cccggaaatc gcccgcgatt tcagtaacgt tggcccgatg gttcgcgcca
    18601 gcggtctcgc ccgcgatact cgcgccgatc acccgtttgt cggttatggc ctgctgccaa
    18661 tggaagtcca cagcgagcag ggctgcgacg ttatttcccg tctgaaagtg cgaatcaacg
    18721 aggtctatac cgcgctgaat atgatcgact acggtctgga taacctgccg ggcggtccgc
    18781 tgatggtgga aggctttacc tacattccgc accgtttcgc gctgggcttt gccgaagcgc
    18841 cgcgcggcga cgatatccac tggagcatga ccggcgacaa ccagaagctg taccgctggc
    18901 gctgccgtgc cgcgacctac gcgaactggc cgaccctgcg ctacatgctg cgtggcaata
    18961 ccgtttccga tgcgccgctg attatcggta gcctggaccc ttgctactcc tgtaccgacc
    19021 gcatgaccgt agtcgatgtg cgtaagaaga agagcaaagt ggtgccgtac aaagaactcg
    19081 agcgttacag cattgagcgt aaaaactcgc cgctgaaata aggaatcgcc atgtttacct
    19141 ttatcaaaaa agtcatcaaa accggcacgg cgacctcgtc ttatccgctg gagccgattg
    19201 cggttgataa aaacttccgt ggtaagccag agcagaaccc gcagcagtgc atcggctgcg
    19261 cggcttgcgt caatgcctgc ccgtcaaacg ccttaacggt tgaaactgac ctcgccacag
    19321 gagagctggc gtgggagttt aatctcgggc gctgcatctt ctgtggacgc tgcgaagaag
    19381 tttgcccgac ggcggcgatc aaactgtcgc aagagtacga actggcggtg tggaagaaag
    19441 aaaacttcct gcaacagtcc cgcttcgcgc tgtgcaactg ccgcgtctgc aatcgtcctt
    19501 tcgccgtaca gaaagagatc gactacgcca ttgcgctgct taagcacaat ggtgacagcc
    19561 gcgcggaaaa ccaccgcgaa agctttgaga cttgcccgga atgtaagcgc cagaaatgcc
    19621 tggtgccgtc cgaccgtatt gaactgactc gccatatgaa agaggccatc tgatgagcaa
    19681 tttattaggc ccccgtgacg ccaacggcat tccggtcccg atgacggtgg atgaatccat
    19741 cgccagcatg aaggcgtcgt tactgaaaaa aatcaaacgt tctgcctatg tttaccgcgt
    19801 ggactgcggc ggctgcaacg gctgcgaaat cgaaattttc gccacgcttt ctccgctgtt
    19861 cgatgcagaa cgcttcggca ttaaagtcgt tccttcaccg cgtcatgcgg atattttact
    19921 gtttaccggc gcggtcaccc gtgcaatgcg atcccctgcg ctgcgtgcgt ggcagtccgc
    19981 gccggacccg aaaatctgta tctcatacgg tgcctgcggt aacagcggcg ggatcttaca
    20041 cgatctctac tgcgtgtggg gcggtacgga taaaattgtc cctgtggatg tttatatccc
    20101 tggctgcccg ccaacgcctg ccgccacgct gtacggcttt gcaatggcgc tcggcctgct
    20161 ggagcagaaa attcacgccc gtgggccggg tgaactggat gaacaaccgg cggagatcct
    20221 gcatggcgat atggtgcagc cgctgcgcgt gaaagtggat cgcgaagcac gtcgcctggc
    20281 gggttatcgt tacggtcgtc agattgccga tgattacctt acacagttag ggcagggcga
    20341 agaacaggtt gcacgctggc tggaagcgga aaacgatccg cgtctgaacg agattgtcag
    20401 ccatctgaat catgttgttg aagaggcgcg tatccgatga gtgaaaaggt ggtgttcagt
    20461 caactgagcc gtaaatttat tgatgagaac gatgccacgc ccgccgaggc gcagcaggtg
    20521 gtctattaca gcctggcgat tggtcaccac cttggggtta tcgattgcct ggaagcggcg
    20581 ctcacctgcc catgggatga atatctggca tggattgcca ctctggaggc gggcagcgaa
    20641 gcccgccaca aaatggaagg cgtaccgaaa tatggcgaga tcgtcatcga cattaaccat
    20701 gtgccgatgc tggccaacgc attcgataaa gcccgggcag cgcaaacttc gcagcagcag
    20761 gaatggagta caatgctgtt aagtatgctg catgatattc atcaggaaaa cgccatctat
    20821 ttgatggtga ggagactgcg tgactgacgt tttactctgt gttggcaata gcatgatggg
    20881 cgatgatggc gctggcccgc tgctggcgga aaagtgcgcc gccgcgccga aaggtaactg
    20941 ggtggtgatt gacggcggta gtgcaccgga aaacgacatc gtcgccatcc gtgaactgcg
    21001 cccgacacga ctgctgattg tcgacgccac ggatatgggg ctaaaccccg gcgaaatccg
    21061 catcatcgac ccggatgata tcgccgagat gtttatgatg actacccata acatgccgtt
    21121 gaattacctt atcgaccagt taaaagaaga cattggtgaa gtgattttcc tcggcattca
    21181 gccggatatc gtcggctttt actacccgat gacccagccg attaaagatg cggtagaaac
    21241 cgtttatcaa cgactggaag gctgggaagg aaatggtgga tttgcgcagt tagcggtgga
    21301 agaagaataa ttctccatca gggaattcag ggaattcagg gaattcaggg aattcaggga
    21361 attgaacatc agaacaggga tgtgcgcttt tttattgagc ttagtgctgc ctgcacaggc
    21421 caccagcttt accgaatatc tgccaatgag tgacagcgaa tacgcgcaaa agcgggcgct
    21481 gaaacctctc ctgaccatgc cttatgatgc cgagcaaacc tggcatttta ggaaagtggg
    21541 cgttgctggc gtgacgcttg agaagatgcc aaatgaagaa gattactggc aattaaccgc
    21601 taaagaccgc gccggtaaat cctggactgt gcctgtgggt atgctggcaa acatagcggg
    21661 caaagggcaa ctctatcgcg ccgatcttga tcgtaacggc attcaggatc tggttatctg
    21721 gttaccaagt accggtaatg gtctggctcc ttatgcacat ttaattctta tgacgttcac
    21781 gcgcgaaggc cgtccctgtg tctttgaacc caggggattc tacaccgcaa gcaaaactgg
    21841 cgtagatgat ttgctggatt tgcaaggcaa tggtcacacg caattgctgg atatgcagtt
    21901 taattctggt tactggataa ccagtctgta tcaggtaaaa gacgccaaat ggcagcgtgt
    21961 tcacggctgg tttgacaagt taagctatcc cgcgctcacg cgttttactt atacccccaa
    22021 ccgtaaactc gtcctcaaac ccatcgccgg gcgcgacccg caaacggaag atctggcgca
    22081 gacgcagcgt tgcttgataa agggggatgt gcttgagggg ggaaccagaa ctgacttttt
    22141 gcagagtggc tatgaggatt ttgggggtaa ttatatcgcg cacaagtgtt tacatattgt
    22201 gaacttggat ttgatgagct atagtttccg aacacataag gatagaactc gcagatgtgg
    22261 ctgtttactt actataagga agttaaaact taaaaatgca catcatttct aaagcgccat
    22321 ttgaagagtc tgcgcgtaaa tatcctaacg acgctctggc tcttcaggca ttgtatcggg
    22381 ttattaagga aacggatttt tctacgccag aagagatgcg aacggcgttt cccaacctcg
    22441 acaattttaa atatcgcaac aaatggtacg ttctggatgt aggtgggaac aacctagggg
    22501 tgattgccta cataaacttt gttaataagc ggttctttgt gaagcacata actaatcacg
    22561 ccgagtatga caaactgaca cgttactaca gagagaacaa agaatgactg ctaatgctgc
    22621 acacgctgtt aaagcaacaa gagaactggt caatgcagtc ccctttctgg gaggtagtga
    22681 ctcagaagat gattatcgtg aagcactgga actggtcgaa tatttgattg aagaagatga
    22741 caccaatcca cttatcgact ttctggcgag ccgcattgca gaatatgaaa acaacaacca
    22801 agagtttgca gagttcgaca aagccgttgc ggcaatgcct gttggcgtgg cgttacttcg
    22861 cacgctgatt gatcaacata atctgaccta tgcggatttg aagaacgaaa tcggctctaa
    22921 atccctggtc agtcagattt tgtctggaca acgatccctg acgatatccc acattaaggc
    22981 actttcagcc cgatttggcg ttaagccgga atggtttctt tgatttttga ctaatgccaa
    23041 atgctaaatt gcccgatgcg ctgcgcttat cgggcctaca tgtcgcacga actatgtagg
    23101 ccggataagg cgttcacgcc gcatccggca ctgttactta ctctaaatct tccccattac
    23161 tggcaatcac ttttttatac caccagaacg atttcttacg tgtgcgcgtc agcgtgccgt
    23221 tgcctgcgtc gtcacggtct acgtagacaa aaccgtagcg tttgctcatt tcacccgtag
    23281 aggcggaaac taaatcaata cagccccatg tggtgtagcc catcagcgga atgccatctg
    23341 caatcgcttc gcctattgcg cggatatgtt cgcgtaaata gctgatgcga tagtcatcgt
    23401 taatctcgcc attggcagca agttcatctt ttgcgccgag gccgttttcc accagaaaca
    23461 gcggcttctg ataacgatcg tacatcatat tcatggtgat acgcagaccg agtggatcaa
    23521 ttccccagcc ccagtcgctc acttgcagat acgggttacg cagcgatttc accacgttcg
    23581 ccgcactgct gttgttggcg ttcatctccg ccgaggcgca gcgcgaggcg taatagctga
    23641 aagagacaaa atcgacggtg tttttcagga tctcatcatc gcccagcgct ttatctatgg
    23701 ttaccccttt ttcgcgaaat acgcgggcag agtaagccgg atacgcgccg cgcgcctgca
    23761 catcgataaa aaacaggttt tcccgatctt tctccagcgc cgcccagaca tcttccggtt
    23821 tgcagctgta agggtagaag ttaccgcccg ccagcataca gcccacctga ttttgcgggt
    23881 taacctcatg ggcgattttg gtggctagcg cactggcaac cagctggtga tgcgcggcct
    23941 gatatttcac ctgatcctga ttttcacctt cttcaaacac cagacccgcg ccggagaacg
    24001 ggctatgcaa cataatgttg atttcattga aggttagcca gtatttcacc agaccatcaa
    24061 atgcttcaaa gcaggttctg gcgtagcggc tgaaaaactc caccagcttg cggttacgcc
    24121 aggagccata ttcggtgacc agatgcatcg gcacatcgaa gtggcacaac gtgaccagcg
    24181 gttcgatacc gtacttttta cactcttcaa agacaggacg ataaaaagca atgccctgct
    24241 gattgggcgt gatttcatcg tcctgcggaa agagacggct ccaggcaatt gaggtacgga
    24301 aaaccttgaa tcccatctct gccatcaggg cgatatcttc tttataacga tgataaaaat
    24361 ccgtcgcctc atggctggga taaaactcgt catcgcgcaa ctgaaaacgt ttttccagcc
    24421 ccagtttcac cgccattcga tgctcgccgt gtgggatcat atcgacggtg gtcagccctt
    24481 ttccgccttc acggaacgca ccttcagact ggttggcggc aagcgcgccg ccccataaaa
    24541 aaccttctgg aaatactgac attttcatcc tcaattaaga cttacttctt tggcgacggt
    24601 cggttgatgc tttcgcgcct gggcagccgc ttcctcaaca ggaatatcct caaagccgag
    24661 caacagtgtg aggataaacg acagcaccac cgccagcgcc atgaccgcga acacccagac
    24721 gatgctcatt ggattcgccg gatcgaagaa ttgcacgctg gtaaatagcc ccggcgctgc
    24781 cattgagtgg ctggcaagcc ccgccatacc ggcaaccgcg ccgcaaataa aaccgctgat
    24841 aagactggcg ataagcggac gtttcaggcg gatcgccacg ccgtataacg ccggttcgga
    24901 aatccccgcc ataatggctg atgccgccgc agccagcgcc gtctggcgca gttccgggtt
    24961 tttcgttttc cacggcaccg ccagtgatga accgcccagc gacaggttag cgccgatctc
    25021 tgatggcatg accattcctt ctttgccggt ttcggcaatg gtctgaatga tggttggcgt
    25081 aaagacgcgg tgcatcccgg tcattaccag cagaggccac agcgcgccca taatggcgac
    25141 tgaaagccag cccagataac catgaatggt gtaaaccagc gccgaaatgg cgctaccgat
    25201 ccagatacca atcgggccaa tcagcaggat tgccagcggt gcggcaatca acacaatcag
    25261 cattggcttg aggaagtttt tggtcaccgc cggtgtaatg cgatccaccc agcgttcgat
    25321 atatgacagg caccaggtca tcaccagcgc cgggatcacc gtgtaggtgt atttcaccgc
    25381 ggtgaccggg atcagggcaa attcgacatg ttcaccctgg gccgctttcg ccattaattc
    25441 aataaaactc ggatgtacca gcacacccgc gatggcaatc gccagcgaca tattggtttt
    25501 aaatttgatg gcggcagatg ccgcgaccat tagcggcagg aagaagaaag caccgtcacc
    25561 aatcacattc agaatggtta aggtcggcga tccttttgtc agcacgccgc tcatctcgag
    25621 gatcatcgcc agcagtttga ccatcgatcc gccgataatc gccgggatca gcggtgacat
    25681 ggtgccgatc agcgcatcga ggatccccgc accaatgcga cgcagcgtga gtttgggttt
    25741 acccacgggc tgtgcgggct gcatatctcc cggcagcagg ctgacgactt cctgaaaggc
    25801 ctgcgaaacc gtattgccga taattacctg acactggttg tcactacgta ccacgccgag
    25861 cacgccgggg atggttttta acgtcgggct gtcgataagc tggtcatctt tgataacaaa
    25921 gcgcaaacgc gtcatacagt gcgtgactgc cgagatgtta tcaacgccgc ccagtgccgc
    25981 tatcaccgag cgtgccagcg ccgcataatt tttggccatc ggattttatc ctgttatcag
    26041 tagggtagat gtagttgtta tcagtcgacg tgacggaaaa ataccgatgc tgaaacggaa
    26101 ttaagaaacc ggtttcacaa aatcatgaat agttgtcact aaataagcaa gagagttaat
    26161 tgttcatttt ttatatttgt gaaaccggtc acctgaatat ttttcccgcc gattgcgcaa
    26221 ggacaatcgg caaacggtgc agtacactgc gttgattgcg tgtaaaggga tgaaaatgat
    26281 gacgacgatg ctggaagtgg cgaagcgcgc cggggtttca aaagcgaccg tttcccgcgt
    26341 gctttcaggt aatggctacg tcagccagga gactaaagat cgcgtgtttc aggcggtaga
    26401 agagagcagt taccgcccaa atttgctggc ccgcaatctg tcggcgaaga gtactcagac
    26461 gctggggctg gtagtgacca acacgcttta tcacggcatt tattttagtg aattactgtt
    26521 tcatgccgcg cgaatggcgg aagagaaagg gcgacagttg ctattggcag atggtaaaca
    26581 cagcgccgaa gaagagcgcc aggcgattca gtatctgctg gatttgcgct gcgacgccat
    26641 tatgatttac ccgcgttttt taagcgtgga tgagattgat gacatcattg acgcgcacag
    26701 tcagccgata atggtgctta atcgccgcct gcgcaataat agcagccata gcgtctggtg
    26761 cgatcataaa cagaccagct ttaacgccgt ggcagagttg ataaacgccg ggcatcagga
    26821 gattgctttc cttaccggct cgatggattc ccccaccagc attgaacgtc ttgccgggta
    26881 taaagacgcg ctgtcgcagc atggtattgc gctcaatgaa aaacttatcg ctaacggtaa
    26941 atggacgccc gccagcgggg ccgaaggggt agaaacgttg ctcgaacgtg gggctaaatt
    27001 tagcgcgtta gttgccagta acgacgatat ggcgataggt gcgatcaaag c
//
LOCUS       DAJTRQ010000042        26286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000042 DAJTRQ010000000
VERSION     DAJTRQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            164..1147
                     /locus_tag="OO332_001879"
     CDS             164..1147
                     /locus_tag="OO332_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5942379.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1170..2681
                     /locus_tag="OO332_001880"
     CDS             1170..2681
                     /locus_tag="OO332_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5942380.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2706..3704
                     /locus_tag="OO332_001881"
     CDS             2706..3704
                     /locus_tag="OO332_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5942381.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3737..4831
                     /locus_tag="OO332_001882"
     CDS             3737..4831
                     /locus_tag="OO332_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5942382.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4843..5715
                     /locus_tag="OO332_001883"
     CDS             4843..5715
                     /locus_tag="OO332_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5942383.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5763..6185)
                     /locus_tag="OO332_001884"
     CDS             complement(5763..6185)
                     /locus_tag="OO332_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5942384.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6282..7484)
                     /locus_tag="OO332_001885"
                     /pseudo
     CDS             complement(6282..7484)
                     /locus_tag="OO332_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OO332_001886"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OO332_001886"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5942385.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8303..8623)
                     /locus_tag="OO332_001887"
     CDS             complement(8303..8623)
                     /locus_tag="OO332_001887"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5942386.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8623..9141)
                     /locus_tag="OO332_001888"
     CDS             complement(8623..9141)
                     /locus_tag="OO332_001888"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5942387.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9138..10499)
                     /locus_tag="OO332_001889"
     CDS             complement(9138..10499)
                     /locus_tag="OO332_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5942388.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10635..11525
                     /gene="hcaR"
                     /locus_tag="OO332_001890"
     CDS             10635..11525
                     /gene="hcaR"
                     /locus_tag="OO332_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5942389.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11685..12824
                     /locus_tag="OO332_001891"
     CDS             11685..12824
                     /locus_tag="OO332_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5942390.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OO332_001892"
     CDS             complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OO332_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5942391.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14287..15141)
                     /locus_tag="OO332_001893"
     CDS             complement(14287..15141)
                     /locus_tag="OO332_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5942392.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OO332_001894"
     CDS             complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OO332_001894"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5942393.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16208..16948
                     /gene="trmJ"
                     /locus_tag="OO332_001895"
     CDS             16208..16948
                     /gene="trmJ"
                     /locus_tag="OO332_001895"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5942394.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17218..17706
                     /gene="iscR"
                     /locus_tag="OO332_001896"
     CDS             17218..17706
                     /gene="iscR"
                     /locus_tag="OO332_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5942395.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17818..19032
                     /gene="iscS"
                     /locus_tag="OO332_001897"
     CDS             17818..19032
                     /gene="iscS"
                     /locus_tag="OO332_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5942396.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19060..19446
                     /gene="iscU"
                     /locus_tag="OO332_001898"
     CDS             19060..19446
                     /gene="iscU"
                     /locus_tag="OO332_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5942397.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19463..19786
                     /gene="iscA"
                     /locus_tag="OO332_001899"
     CDS             19463..19786
                     /gene="iscA"
                     /locus_tag="OO332_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5942398.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19882..20397
                     /gene="hscB"
                     /locus_tag="OO332_001900"
     CDS             19882..20397
                     /gene="hscB"
                     /locus_tag="OO332_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5942399.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20414..22264
                     /gene="hscA"
                     /locus_tag="OO332_001901"
     CDS             20414..22264
                     /gene="hscA"
                     /locus_tag="OO332_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5942400.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22266..22601
                     /gene="fdx"
                     /locus_tag="OO332_001902"
     CDS             22266..22601
                     /gene="fdx"
                     /locus_tag="OO332_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5942401.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22613..22813
                     /gene="iscX"
                     /locus_tag="OO332_001903"
     CDS             22613..22813
                     /gene="iscX"
                     /locus_tag="OO332_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5942402.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22872..24155
                     /gene="pepB"
                     /locus_tag="OO332_001904"
     CDS             22872..24155
                     /gene="pepB"
                     /locus_tag="OO332_001904"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5942403.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24297..25073
                     /gene="sseB"
                     /locus_tag="OO332_001905"
     CDS             24297..25073
                     /gene="sseB"
                     /locus_tag="OO332_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5942404.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25568..26050)
                     /gene="sseA"
                     /locus_tag="OO332_001906"
                     /pseudo
     CDS             complement(25568..26050)
                     /gene="sseA"
                     /locus_tag="OO332_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6214 a         6580 c         7296 g         6196 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccctacgt
       61 tcactaaacc ctattaactg taatatttga gcggcacgcg ctgccgctca tcacaaaata
      121 attagaataa tccgttgtta ataaaaaccc tacaggaggc actatgccta aaaaaatgag
      181 aacaacccgt aatctattat taatggctac gctattaggt agcgcactat ttgccagggc
      241 tgcggataaa gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg
      301 agtgcgtcag ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga
      361 aactaacgcc cagggggata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg
      421 taatgtcgat gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg
      481 tcgcgccagt gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg
      541 tgtcgataaa tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact
      601 gggtaacgct gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt
      661 catcaattgc gaagcctttg aagtttgtgt gcagcgacgt aaaggatttg aagaagtatt
      721 aaaatcccgc gttcccggcg cgcaaattgt cgctaaccaa gaagggactg ttttagataa
      781 agcgatttcc gttggtgaaa aactgattat ctccacgccg gatctcaacg ccattatggg
      841 ggagtcgggc ggtgcgacac tcggcgcggt aaaagcggta cgtaatcaaa atcaggccgg
      901 aaaaattgct gttttcggtt cggatatgac aaccgaaatt gctcaggagc tggaaaacaa
      961 tcaggtgctg aaagcggtag tggatatttc cggtaagaaa atgggcaatg ctgttttcgc
     1021 gcaaacattg aaggttatca ataaacaagc cgacggtgaa aaagtgattc aggtgccaat
     1081 cgatctctat accaaaacgg aagatggtaa acagtggctg gcaacgcacg ttgatggtct
     1141 gccctaatcg ctgattcgca gaggtcccta tgttcacggc aacagaggca gtcccggtag
     1201 caaaagtggt ggcaggaaat aagcgttacc ccggcgtcgt tgcgctggat aacgttaact
     1261 tcacgctcaa taaaggcgaa gttcgtgcgc tgttaggtaa aaacggcgcg ggcaaatcga
     1321 ctctcattcg aatgcttacc ggcagcgaac gcccggatag cggtgatatc tggattggcg
     1381 agacgcgact ggaaggtgac gaagctacgt tgactcgccg tgccgctgaa ctgggggttc
     1441 gtgcggttta tcaggaatta agtctggtgg aagggctgac ggtggcagaa aatctctgcc
     1501 ttggtcaatg gcctcgccgc aacggcatga ttgattacct gcaaatggcg caggatgccc
     1561 aacgttgctt acaggcgctg ggcgttgacg ttagtcctga acaacttgtt tcaacgctaa
     1621 gcccggcgca aaagcaactg gtggaaattg cacgggtgat gaagggcgag ccgcgcgtgg
     1681 tcattcttga tgaacccacc agctcgcttg ccagtgcaga agttgaactg gtgatcagtg
     1741 cggtgaaaaa gatgtcggca ctgggcgtgg cggtgattta tgtcagccac cggatggaag
     1801 aaatccgccg cattgcctcc tgtgccaccg ttatgcgcga tggtcaggtg gcgggcgatg
     1861 tgatgctcga aaacacctcc acgcatcata ttgtgtcgct aatgctaggg cgtgatcacg
     1921 ttgatattgc gccggttgcc cctcaggaaa ttgtggatca ggccgtactg gaagtccgtg
     1981 cgttacgcca taagcccaag ctggaggata tcagctttac gctacgtcgt ggcgaagtgc
     2041 ttggcattgc tggcctgctg ggggcagggc gcagtgaatt gctgaaggcg attgttgggc
     2101 tggagacgta tgaacagggc gaaattgtta tcaacggcga gaaaatcatg cgccccgatt
     2161 acggcgacat gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gaagcgggga
     2221 tcattccctg gctgggcgtt gacgaaaata cagtgctgac caatcggcaa aaaatcagca
     2281 ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga
     2341 tgacggtcaa ggctgccagt agcgaaacac ccatcggcac gctttctggt ggcaatcagc
     2401 aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc
     2461 caacgcgcgg cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgtgagctgg
     2521 ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt ggaggaattg ccgctggtgt
     2581 gtgaccgcat cctgttatta cagcacggca cgttctcgca ggagtttcac tcgccggtca
     2641 atgtggatga gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc
     2701 tgaagatgtc tgcttcgtca ttaccattgc cgcagggcaa gagcgtctcg ctcaaacaat
     2761 ttgtcagtcg ccatattaat gagatcggtt tgctggtggt gattgccatt ctttatctgg
     2821 tcttctccct gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg
     2881 atgccgccac cattgggatt gccgcctggg cgatgacgct gattattatc tccggtgaaa
     2941 ttgatgtcag cgttgggccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc
     3001 aatttgaagt tccgttggcg attgcttgtc tgctggtgtt gctgttaggc gcgctgatgg
     3061 gaacgctcgc cggggtgctg cgcggcgtgt ttaacgtacc aagtttcgtt gccacactgg
     3121 ggctgtggag cgctctgcgc ggaatggggc tgtttatgac caacgcacag ccagtgccga
     3181 ttgacgaaaa cgaggtgctg gactggctgg gcggacaatt tctcggtgtg ccagtatccg
     3241 cgctgatcat gatggtgttg tttgcgctgt ttgtgttcat tagccgaaaa accgccttcg
     3301 ggcgctcggt ttttgccgtt ggcggtaatg ccacggcggc gcagttgtgc ggtattaacg
     3361 ttcgtcgagt gcgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca
     3421 ttttgttggc ggcacgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg
     3481 acgtcatcgc cgcggtcgtg gtcggcggta cggcactttc cggcggtcgc ggttccctgt
     3541 tcggtacatt gcttggtgtg ctggtgatta cgctaatcgg taacggtctg gtgctgctcg
     3601 gtattaactc ctttttccag caggtggtac gcggcgtcat catcgtggtg gcggtgctgg
     3661 cgaatatctt gctgacccag cgaagcagta aagcgaaacg ctaaacctta acgcccctgg
     3721 cgcgggtgcg gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat
     3781 gctggcagct tatttaccag gaaattcaac cgtcgatctg cgggaagttg cggtgccgac
     3841 gccggggatt aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt
     3901 ccactatatc tatcatcaac accgtgccac ggcggcggca cccgataaac cgttatacca
     3961 gggatttatc aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctaccg
     4021 ccattttaaa gagggcgacc gcgtgctggt gtatcacatt tctggctgtg gtttttgccc
     4081 gaactgccgc cgcggctttc ctatctcttg tactggcaaa ggaaaagcgg cttacggctg
     4141 gcagcgtgac ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct
     4201 gccggatgcg ctgagctacg aagatggtgc gtttatcagt tgcggcgttg gcacggccta
     4261 tgaagggatt ttgcgcggcg aagtttccgg cagcgataac gtgctggtgg tcggtctggg
     4321 ggcggtcggc atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaac ggatcatcgg
     4381 cgttgatatg ctgccggaac gtctggcgat ggcaaaacag ttagggggga tggatcacgg
     4441 ctatttagca accaccgaag gtctgccgca gataattgct gaacttaccc acggtggcgc
     4501 ggatgttgcg ctcgattgtt ccggtaatgc cgcaggtcgc ttgctggcac tgcaatccac
     4561 tgctgactgg gggcgggtgg tttacattgg tgaaaccgga aaagtggaat tcgaggtcag
     4621 cgctgatctg atgcaccatc aacggcggat tatcggctcc tgggtgacca gtctgttcca
     4681 tatggaaaaa tgcgcccacg atttgactga ctggaagctg tggccgcgta acgccattac
     4741 ccatcgcttc tcgctagaac aggcaggtga tgcctatgtg ctgatggcga gcggcaaatg
     4801 cgggaaagtt gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta
     4861 tcccacgggt cgctaaagct ggacgtttct gaccagggcg gcgtaatcga aggcttctgg
     4921 cgcgatacga cgccgctctt acgtcctggt aaaaaaagcg gtgtggcaac cgatgcctca
     4981 tgttttccgc tggtgccatt tgcaaaccgg gtgagcggca accgttttgt ctggcagggg
     5041 cgtgagtatc aactgcaacc gaatgttgag tgggatgcgc actatctaca cggcgatggc
     5101 tggctgggcg agtggcaatg tgtctcgcac agcgatgata gtttgtgtct ggtgtatgaa
     5161 catcgcagcg gtgtctatca ctatcgggta agtcaggcgt ttcatttaac ggcggatacg
     5221 ctgacggtga cgctctctgt caccaatcaa ggggcagaga cgctgccatt tggtaccggc
     5281 tggcatcctt attttccgtt gtcaccacaa acgcggattc aggcgcaggc gagcggttac
     5341 tggctggaac gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaggaactg
     5401 gattttaacc agctcgcgcc gttgccgcgt cagtgggtga acaatggttt tgccggatgg
     5461 aatggtcagg cccgcatcga gcagccgcag gaaggttatg ccatcatcat ggaaacgacg
     5521 ccacctgcac cgtgttattt catctttgtt tccgaccctg cgtttgataa aggatatgcg
     5581 tttgatttct tctgtctgga accgatgagc catgcgccgg atgatcatca tcgcccggaa
     5641 gggggtgatc tcattgcgct agcaccaggg gaatcaacaa cctcagagat gtcgttgcgg
     5701 gtagagtggc tgtaatttat aaaatgccgg ataaggcgtt cacgccgcat ccggcatctt
     5761 tcctaacgcc gatcgagaga aattgccccc ggtccggtaa ttgccaatag caagaacgcg
     5821 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
     5881 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
     5941 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
     6001 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
     6061 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
     6121 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acgtagtgtg
     6181 ttcataacca atccctttgc aagataatgt gatttaaacg taatacacac tcttgcacct
     6241 cgccacgccc caggcattga gatattcgct aattatcctg gttacagtga tttaagcgcg
     6301 atgttctcat ctatcagcag tttcgcatca aacgttttgc cgctctggat ccatttgcga
     6361 attgggcgaa tctcacgccc ctgattcagc gtgaccgcac cgataagcac gccgttttgc
     6421 agattaaacc aaatcgcctt ctgagtttct gggttgccac gacaaagcta gtcatcgcca
     6481 cgcatatcgc caataaactg taagttatca ctgtactgat cgctccagaa ccacggcggc
     6541 ggcagtagcg gtagcggtag ccctaacatt gcggcagcgg caatttgcgc ctggttattg
     6601 gcgttttccc agctttcgca gcggtgtagt gcaccattat caagacgagt gattgccaca
     6661 tcgccaccgg caaagatcgc gggatcgcag gtgcggcaag cctcatcaat gacaatgcca
     6721 ttggcagtat caaggttggc ctcgcgagcc agttgctcgt tggcgctgat actaataccg
     6781 taaatcacca catcagcctg aagcgtttcc ccactttgca gcgtcagttc tactttttca
     6841 ccatcgacca catgttcaat ggcattattg agcagaatgc gcacaccagc ctgctgatgg
     6901 cgctgtaaaa gatagcgttg cacgggcggt ggtgcattac ggcccatgac ggttgccgcc
     6961 agttcaatca ctgtcacctt acatctgcgc tgcgtggcgc tggcagccag ttccagacca
     7021 atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc tcgcagtctg
     7081 gcggcatcac cggcatggtg tagggtaaag cagcgttctc ccagtgcatc aagcaacggc
     7141 agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca gctttcgccg
     7201 ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac accggaatgc
     7261 agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa
     7321 tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg
     7381 gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc agccattgcc
     7441 gccgcttgcc cgccaccgac aataatgatc gttttttctt tcatcgtgct tccttaaaga
     7501 tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc
     7561 atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata cggccccgta
     7621 aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc
     7681 agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg
     7741 gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc ataagccagt
     7801 tggcgaataa gtccggttgc ggcatgttta ctggtggtgt acagcgggcc accgccgcca
     7861 ggataccagg cggcatttga cagtgtgaaa atcatgctgc cttcactggc gattaacgcc
     7921 ggagcgcagg cttttgcgcc cagcaggtaa ccgagtacgt tgacgttaaa cagctcgtgg
     7981 aagccggttt cgagcgtctc tgcgggagta ttaaccagtg aggcattgtg atcccagatg
     8041 cctgcattgc cgataaaaca atccagcttg ccggaacgag tcaggatctg atcgaccgcg
     8101 cgttgataat cggcataaca ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat
     8161 cgctgacgca gactggcgac ttttgccgcc gacagttcca gcgcggcaac ctgcgcgcct
     8221 tcttcgataa atcgctcgac cagcgccagc cctaatcccg atccgccgcc ggtaataaaa
     8281 atggactcgt tatgcagatc gctcatggct gcgcctccgg taagtcgatg aaaatgtcac
     8341 caccttcaac gtgtactgga taagtggtga gcggatcggt ggcgggcagg cataacgctt
     8401 tccccgtttt caggcaaaaa ctggcggcgt gtagcgggca ctccaccgtg gcgtcatctt
     8461 ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta atggcataaa
     8521 actcgccgcc cacgttgaac aaggcgatga cgggcgaggt atctatccgg agagcctcac
     8581 cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca gtacactcag
     8641 gttatgggaa gtgatcaccg cttgatccag gacgatatcc cgttccagca agcgccagtt
     8701 gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg
     8761 ctctttttgt gcccgataaa gtagataatt caccctcacg gcaaatacgt ttgggatgtc
     8821 ggtttcgctt acctggcagt tgctgattaa gtgacgagtg cgtgacggcg gctcttctgc
     8881 ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt tggtgtcatt
     8941 gaaaatccag gtcgtcggcg gctggacgcc tttgcgacgg tcgcgtgttt gcgcgttaac
     9001 cgtggtgcgc atggtgtaac gaatctcttc gtcgagctgt gccaaccagt cacgaaattt
     9061 ccagtcatcc agcagactgg cttcgtgaaa gagaaactgg ctaatgcggt gatgtaactc
     9121 cagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt tttatcgagc
     9181 acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat tccacgagcg
     9241 gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg gcgcttttcc
     9301 tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc tttaagcaat
     9361 ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa
     9421 gcacgagtgg cgctgttttc aaaagcggct ttaacttcat cggaggcggc tttgtcagta
     9481 atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac gcggagtgtg
     9541 gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc caggcgcagg
     9601 gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta atagcttgaa
     9661 actgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa accgctaccg
     9721 tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg gcgggcggtt
     9781 tgtccatcac cgaggcgctt atcgctgcca tcatctttcg cccctaatac ctgaacggca
     9841 gaagcatggc tgaacagagc atgatactgg tcactggcga actgctctgc cgggaatttc
     9901 cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc gccttcgcga
     9961 cgatccagca tgccatccag ataccaggca atgtcaccca ggtaatcatg caggcccggt
    10021 gcgctggtat cccagttgcc aaaaatcagt cctttataac tctccacaca aggaacttcg
    10081 tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc cagcggtaca
    10141 tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt aaaggcgcga
    10201 gtgttgccac aatctgcata actcacacgc atggcccggt ggcggcattg gttaagaaac
    10261 gccttgatgc tgccgtcttt ctggcgcacc acgacaactg catcttctcc catgtaggtg
    10321 ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag ccagcaacga
    10381 ccgaaaatac gctcaagctc cagttggtaa atgtccgggt cggtataaat acgcggagtg
    10441 acccgaccgt tttgggtgtc aatcagttgg taaatgttca aatctgaggg tgtggtcata
    10501 ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat aaggatgttg
    10561 ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt tttttcgata
    10621 acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg gcgcaggcac
    10681 tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta agcagccaga
    10741 tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag cgcaaagtcg
    10801 cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg
    10861 aaaatgccaa attacgggcg cggaaaattg ttcaggaaga cagacaatta accattggct
    10921 ttgtgccatc ggcggaagtg aatttattgc caaaagtatt accgatgttt cgtctcagac
    10981 agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag gaaaaaattc
    11041 gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg gagattgatt
    11101 atctggagct ttttgacgaa ccattagtgg tggtgttacc ggttgatcac cctttagcac
    11161 atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt accgatccgg
    11221 cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc
    11281 caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg ggcatggggc
    11341 tgggcgtcac tttgataccc ggttatatga ataattttaa taccgggcag gttgtttttc
    11401 gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag aaaggggaga
    11461 tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg
    11521 cataattacg cattattttt gcaacatgcg ccgcggatcg cagtttttta gtgcttgtga
    11581 actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa cttgcaccaa
    11641 cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt ttgcaatcca
    11701 cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt tttttacctt
    11761 tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc ctgttattgg
    11821 gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc gtcagcgatc
    11881 cttcccgcct gatttccgcc ttgcgcgtgc tggcactgct gccacttctc tttgctgtcg
    11941 ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc tttaacctct
    12001 ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc
    12061 cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt ggctcggcgc
    12121 tgacgggcaa actggtcact atgtttgatt atcgggtgat cctcgtgctg ttgacgttgg
    12181 gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca caaggggcaa
    12241 gccgccagca ggagagcacc ggttggtctg cgtggttggc gctggttcgc cagaactggc
    12301 gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac ggttttagcg
    12361 ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg tggtcgctgg
    12421 gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc cgttgtagtg
    12481 ctcgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc attatgggag
    12541 caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc accttcacgg
    12601 tctgccacct ggccgccatg cgttatattg ctgctcgcca ggttagcgaa gtcatccgtt
    12661 tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat cgctatcatg accgttttcg
    12721 ccggtttcct gtatcaatat ctgggccacg gtgtgttctg ggtaatggcg ctggtggcgc
    12781 ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag cattttgcgg
    12841 atctgctcct gttgaggtgt tgtcagcgtc aggtccgtat agatcagcgg tggtgaaaag
    12901 agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccacgcgg agcgcctgtc
    12961 tgcaaaaatg cctttaccga catatgctta atgttgagcg gcagtaacgt tagttcgcgt
    13021 aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtcagtag aataatctgt
    13081 ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc gaccagacca
    13141 ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag cgccgcgcgg
    13201 gtgatgcgta ccaggcgtgg atacagtctg gcgaactctt ccggcagggt attatcaata
    13261 ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt ggatataaag ctgatcacat
    13321 aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt taccagacgt
    13381 ttgatagact gacgcaattg tctgtcccgc tgatgcgcat cccgcagtgg atcgggaacc
    13441 cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac atgctggagc
    13501 gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag gcaggattcc
    13561 gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg ctgcagcaaa
    13621 caatattgca gatacagaca caggaaatga atatcgcgcg attcaaagcg tttttgcagc
    13681 cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa gttacgcgca
    13741 attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt aataaatgac
    13801 ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg gttaagcact
    13861 gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg atgatagcgc
    13921 aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc cacaccatta
    13981 agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt cagcaagatc
    14041 tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg catcatctgt
    14101 agatctccca taagcataat tgataagaat agctaaagtt tgctcaggga aatggcgaag
    14161 ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat tttaaactgt
    14221 tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg tgctagcgat
    14281 catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct gttcgcgata
    14341 aacattgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa
    14401 ccgttttggc tcttttgcca ggctatacaa cttttcgctg tgctgccatg ggataacgtg
    14461 atcggcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga tgtaattttc
    14521 gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg agcatctggt tggcgatggt
    14581 tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac gatcaccctg
    14641 accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca ccagccgttg
    14701 tgggtttaca tcactgcgat ggcgcaccac attgatggcg ctttgcgtat cgtccagcaa
    14761 tccggcctgg gacggagtgc cttttgattt accaaacccg cgataatcaa acataaaaac
    14821 gttgaaatta cgctcaggta accaactgac cagcggccag tgggcggaca tatttccggc
    14881 attgccgtga gcatgaatga tggttgcgat ggcgttgtca gcagggcccg tcgaagaagg
    14941 aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg attccgccga
    15001 ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga tggcaacgcg
    15061 cagcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt tgggaacgcg
    15121 tttgttcact ggcagtgcca taacctttcc ttgcgacttc tttatcatcc attaatcggg
    15181 gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt tcggaatagc
    15241 gcctgaaagg cgagggcggg tgagtgatat cacccgcctg agtcattaac gcttcagagc
    15301 gtcgcttaac tcgtcacgca tgttcgccag tatggctttc acaacacgcg ggttaccagc
    15361 aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga cgatgccgcc
    15421 cgcttcacga accagcagat cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa
    15481 gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag aaccggtacg
    15541 acggaagtct gcacattcgt tgaacagctt gccgacgatg ttgatgtagg tagtggcgta
    15601 ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat cgcgagcggt
    15661 gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg cggtgaacag
    15721 ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga tacgaacggc
    15781 gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat ccagtggatc
    15841 gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt cggtgatgat
    15901 ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcgg cagctttatc
    15961 tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt ccggggtttc
    16021 atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat
    16081 cggatgcatc agtctctctc actggatgtt aaagaacggg aaaacggcgc agagtatagc
    16141 agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct ttagatgaag
    16201 aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca ccggcaatat
    16261 gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc tggttaatcc
    16321 actggtgaaa cccgactccc aggcgattgc cctggctgca ggggccagcg atgtgattgg
    16381 taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagcc tggtggtggg
    16441 caccagcgca cgctcccgca cgctgccgtg gccgatgctc gacccgcgcg aatgcggtct
    16501 gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc gtgagcgcgt
    16561 cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg cggcaaaccc
    16621 ggaatacagt tcgctgaacc tggcaatggc ggttcaggtt attgcctatg aagtgcgtat
    16681 ggcctggctg gcaactcagg aaaatggcga gcaggtcgag catgaagaga cgccgtatcc
    16741 gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc tggcaaccgg
    16801 ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg
    16861 tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt ctattgagca
    16921 gcagaataaa ggtaacaagg ccgaataaca gtggttgcct gatgcgacgc ttaacgcgtc
    16981 ttatcaggcc tacaatgtac agaatcgtag gtcggataag gtgttcacac cgcatccgac
    17041 aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc gactaaatca
    17101 gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg ctatgcaata
    17161 cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg
    17221 agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc gctcaactct
    17281 gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggaatttc cctttcttat
    17341 ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt acgtggacca
    17401 ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga agtgattagc
    17461 gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg ccagggcggc
    17521 gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac cggttttctc
    17581 aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt gtctggtcgt
    17641 cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt taagttacgc
    17701 gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa acacggtcgt
    17761 acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta tagagcaatg
    17821 aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg tgttgccgag
    17881 aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc tcgttctcac
    17941 cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat tgccgatctg
    18001 gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc tgacaacctg
    18061 gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat caccagcaaa
    18121 accgaacaca aagcggtact ggatacttgc cgtcagctgg agcgcgaagg tttcgaagtc
    18181 acctacctgg caccgcagcg taacggtatt atcgacctga aagaactcga agcggcgatg
    18241 cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag
    18301 gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca cgttgatgca
    18361 acccagagcg tgggcaaact gccaatcgac ctgagccagt tgaaagttga cctgatgtct
    18421 ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt gcgtcgtaaa
    18481 ccgcgtgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg tatgcgttcc
    18541 ggcactctgc ctgttcacca gatcgtcgga atgggcgaag cctatcgcat cgcaaaagaa
    18601 gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc
    18661 aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc gaacattctc
    18721 aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa agacctcgca
    18781 gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg
    18841 ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg tcgttttact
    18901 actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg tcgtctgcgt
    18961 gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag catcgaatgg
    19021 gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag cgaaaaagtt
    19081 atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg ataacaacga cgagaacgtc
    19141 ggcagcggca tggtgggtgc accggcctgt ggcgacgtaa tgaagttgca gattaaagtc
    19201 aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg ttccgctatc
    19261 gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgacga agcgcaggcg
    19321 atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat tcactgttct
    19381 attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa acgtgaagca
    19441 aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag cagcgcgagt
    19501 aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc
    19561 cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc cggaagacat
    19621 cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga
    19681 cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat tcaccaaccc
    19741 gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg cataccgata
    19801 accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt tatcgcggcg
    19861 gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc tgcccgttat
    19921 caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca gtatcatcct
    19981 gataaattcg ccagcggaag cctggcggaa caactcgccg ccgtacagca atctgcaacc
    20041 attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata tttgctttct
    20101 ttacacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc gttcctgatg
    20161 gaacagttgg agctgcgcga agagctggac gagatcgaac aggcgaaaga tgaagcgcgg
    20221 ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca gttgatggtt
    20281 gaacagttag acaacgaggc gtgggacgcg gcggcggata cggtgcgtaa gctgcgtttt
    20341 ctcgataaac tgcggagcag tgccgaacaa ctcgaagaaa aactgctcga tttttaattt
    20401 ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg ctgcgccgca
    20461 tcagcgtcgt ctggcggctg gtattgacct gggcacaacc aactcgctgg tggcgacagt
    20521 gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc tgccatctgt
    20581 tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta acgcagcgct
    20641 tgacaccgcc aacaccatta gttctgttaa acgcctgatg gggcgctcgc tggctgatat
    20701 ccagcaacgc tatccgcatc tgccttatca attccaggcc agcgaaaacg gcctgccgat
    20761 gattgaaacg gcggcggggc tactgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc
    20821 actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtag ttatcaccgt
    20881 tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg
    20941 gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct acgggctgga
    21001 ttccggtcag gaaggcgtga tcgctgttta tgacctcggt ggcgggacgt ttgatatttc
    21061 cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg attccgcgct
    21121 cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg cggacattcc
    21181 tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg cagccaaaat
    21241 cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg gcgaaatcag
    21301 ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac tggcttgtcg
    21361 tcgcgcgctg aaagacgcgg gtgtagaagc tgacgaagtg ctggaagtgg tgatggtggg
    21421 cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg gtcgtccacc
    21481 gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc aggcggatat
    21541 tctggtgggt aacaagccag acagcgaaat gctgctgctt gatgtgatcc cactgtcgct
    21601 gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata ccactattcc
    21661 ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga tgtctatcca
    21721 tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc gttttgcgct
    21781 gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt tccaggtcga
    21841 tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg aggcgtctat
    21901 tcaggtcaaa ccgtcttacg gtctgaccga tagcgaaatc gcttcgatga tcaaagactc
    21961 aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc
    22021 ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc tgttaagcgc
    22081 cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg cgcagggcga
    22141 tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc aggatttcgc
    22201 cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt
    22261 ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat ggcgctgttc
    22321 tggaagctaa tagtggtgaa accattctcg acgcagcgct gcgtaacggt atcgagattg
    22381 aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt cgtgaaggtt
    22441 ttgactcact gccggaaagc tcagagcagg aagacgacat gctggacaaa gcctggggac
    22501 tggagccgga aagccgttta agctgccagg cgcgcgtcac cgacgaagat ttagtggtcg
    22561 aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta gtatgggact
    22621 taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc ccgatcttga
    22681 tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg aagatttcga
    22741 cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag tctggctgga
    22801 cgaagccgag tgaatgaacg ggctgcctgg gggtagcccg ttttgctgtt aaacgaataa
    22861 ggataactaa aatgacagaa gcgatgaaga ttaccctctc tacccaacct gccgacgcgc
    22921 gctggggaga aaaagcaact tacagcatta ataatgacgg cattaccctg catttgaacg
    22981 gggcagacga tctggggctg atccagcgtg cggcccgcaa gattgacggt ctgggcatca
    23041 agcatgttca gttaagcggt gaaggctggg atgcggatcg ctgctgggca ttctggcaag
    23101 gttataaagg cccgaaaggc acgcgtaaag tggagtggcc ggatctggac gatgcccagc
    23161 gccaggaact ggataaccgc ctgatgatca tcgactgggt gcgtgacacc atcaacgcac
    23221 cggcagaaga attgggacca tcgcaactgg cacagcgtgc tgttgatctg atcagcaacg
    23281 tcgcgggcga tcgtgtgact tatcggatca ccaaaggcga agatctgcgt gagcaaggtt
    23341 atatggggct gcacactgtc gggcgcggtt cagaacgttc tccggtattg ctggcgctgg
    23401 attacaaccc aactggcgat aaagaagcac cggtgtacgc gtgcctggta ggtaaaggta
    23461 tcacttttga ctccggcggc tacagcatca aacagactgc gtttatggac tcgatgaagt
    23521 cggacatggg cggcgcggca acggttaccg gggcgctggc atttgccatt acgcgcggac
    23581 tgaacaagcg cgtgaagctg ttcctctgct gtgcggataa cctgattagc ggcaatgcgt
    23641 tcaagctggg cgatatcatc acttatcgca acggtaaaaa agttgaagtg atgaacactg
    23701 atgccgaagg gcgtctggtg cttgccgatg gtctgattga tgccagtgcg cagaaaccgg
    23761 aaatgatcat tgatgcggcg accctcaccg gggcggcgaa aactgcgctg ggtaatgatt
    23821 atcacgcgct gttcagtttt gacgatgcgc ttgccggtcg tttactggcg agtgcctcac
    23881 aagagaacga accattctgg cgtctgccgc tggcggaatt ccaccgcagc cagctgccgt
    23941 ctaactttgc cgaactgaac aataccggaa gcgcggcgta tccggcaggc gcgagcacgg
    24001 cggcgggctt cctgtcgcac tttgttgaga actatcagca aggctggctg catatcgact
    24061 gctcggcgac ttaccgtaaa gcgccggttg aacagtggtc tgcgggcgct acgggacttg
    24121 gtgtgcgcac gatagctaat ctgttaacgg cgtaaactaa tatgccggat gcggcgtaaa
    24181 cgccttatcc ggcctacaaa ttctgcaaat tcaacaaatt gcgaatccct tgtaggcctg
    24241 ataagcgtag cgcatcaggc atttgcgccc cgaacgttga atgaattgtg gttcttatgt
    24301 ccgaaacaaa aaacgaactt gaagacctgc tggaaaaagc agcaaccgaa ccggcgcacc
    24361 gcccggcctt tttccgtact ctactggaat ccaccgtctg ggtgcctggt acggcggcgc
    24421 agggcgaggc tgtggttgaa gattgtgcgc ttgatttaca gcactgggaa aaagaagacg
    24481 gcaccagcgt cattcctttt ttcacctcgt tagaagcgct tcaacaggcg gttgaagacg
    24541 aacaggcatt tgtcgtaatg cccgttcgca cattgtttga gatgaccctt ggcgaaacgc
    24601 tcttccttaa tgccaaactg ccgaccggta aagaatttat gccgcgtgaa atcagtttgt
    24661 tgattggtga agagggaaat ccgctgagca gccaggaaat cctggaaggc ggtgaatcgc
    24721 tgatattatc ggaagtcgca gagccgccag cacaaatgat tgattcactc accaccttat
    24781 ttaaaaccat taagccggtg aagcgtgctt ttatttgttc aattaaagag aacgaagagg
    24841 cacagcctaa tttacttatt ggcattgaag ccgatggtga tatcgaagaa attattcagg
    24901 cgacgggaag tgtagcgacc gatacattac ctggcgatga accaatcgat atttgtcagg
    24961 tgaaaaaagg ggaaaaagga attagccact ttattaccga acatattgcg ccattctatg
    25021 aacgtcgctg gggtggtttt ttgcgtgact ttaaacaaaa ccggataatc taaaatatgc
    25081 cgccctaaag ggcggcattc gggcttactt gctgctctga aggttgttaa ccaccagtaa
    25141 attgtcgccg gagagactcc ggttattatt tacttgggaa aacagcgcac cagaaacaat
    25201 aagtacgata cagaagcacc gtatcttcat tgtgctgtcg tgatgcgaag accgcatcac
    25261 gacagcacca ctggcgatat tgccgcgata gcaagcgctg agacgcgctt gctctaacgg
    25321 tcgcggaaca tcctttcctt aagccagcgc ccgcttgcga gacggcggaa agggccgcag
    25381 tggataatac actaacagaa caataataac tctcttttgc ttgacaaaaa gagagttact
    25441 ggcgagtatt gtctccgctg agacgcgctt gcattactac ggtaatacaa cctgctggaa
    25501 aggccgcgaa ccagaccagc aataaaaaaa cgccaaattc ggcggttttt tattgcctgt
    25561 aaaatactta tttcactggc tcaaccggta aatctgcccg cgcgccccat tcactccatg
    25621 cgccgtcgta cagtttcacg tttggcacat ccagcgtcgc gagtgctaac aaaaccacgg
    25681 ctgccgttac accagagccg cagctgacga taatcggttt gtcatagctg acgccgcgac
    25741 caaaaaatat cgcatccagt tcatcggtcg tttttagttc gccttcgcgc accagttccg
    25801 tccacggaac gttcaatgct ccgggaatat gtccgcgacg taaacctggg cgaggttcat
    25861 caacttctgc gttaaaacgt gcagccgggc gggcatcaat aatttgcgcc gtattttcat
    25921 ggcttgccaa taacacatcg gttactttca ccactgcttc aggattaaac gcggcgttaa
    25981 actctccttc cggcagctct actgcacctt cttctaatag caggtcatcg cgctgccagc
    26041 ctgcaagtcc ccgttacttt attcgtaccc cttataatgg ggtgttagcc agccagaccc
    26101 ggcatgatta ctgcccccag tcgtccatga tccggggggg ggggatgtca ccgggtctgg
    26161 tggggcgctg gtaaccgcta ataggggtca ggtcaggcac ttttgccggg accgtctgta
    26221 acgtggatgc cggtacctgc tccccgtggt tatctggtta acccatatac aagggagaca
    26281 gaatga
//
LOCUS       DAJTRQ010000043        25980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000043 DAJTRQ010000000
VERSION     DAJTRQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(55..195)
                     /locus_tag="OO332_001907"
     CDS             complement(55..195)
                     /locus_tag="OO332_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5942405.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            437..730
                     /locus_tag="OO332_001908"
     CDS             437..730
                     /locus_tag="OO332_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5942406.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            802..1461
                     /locus_tag="OO332_001909"
     CDS             802..1461
                     /locus_tag="OO332_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5942407.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1553..1999
                     /locus_tag="OO332_001910"
     CDS             1553..1999
                     /locus_tag="OO332_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5942408.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2206..3106)
                     /gene="hlyE"
                     /locus_tag="OO332_001911"
                     /pseudo
     CDS             complement(2206..3106)
                     /gene="hlyE"
                     /locus_tag="OO332_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3479..3898
                     /locus_tag="OO332_001912"
     CDS             3479..3898
                     /locus_tag="OO332_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5942409.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3898..5164
                     /gene="umuC"
                     /locus_tag="OO332_001913"
                     /pseudo
     CDS             3898..5164
                     /gene="umuC"
                     /locus_tag="OO332_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(5210..5740)
                     /gene="dsbB"
                     /locus_tag="OO332_001914"
     CDS             complement(5210..5740)
                     /gene="dsbB"
                     /locus_tag="OO332_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5942410.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5886..7427)
                     /gene="nhaB"
                     /locus_tag="OO332_001915"
     CDS             complement(5886..7427)
                     /gene="nhaB"
                     /locus_tag="OO332_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5942411.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7648..8367
                     /gene="fadR"
                     /locus_tag="OO332_001916"
     CDS             7648..8367
                     /gene="fadR"
                     /locus_tag="OO332_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5942412.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8419..9951)
                     /locus_tag="OO332_001917"
     CDS             complement(8419..9951)
                     /locus_tag="OO332_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5942413.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10281..11579
                     /gene="dadA"
                     /locus_tag="OO332_001918"
     CDS             10281..11579
                     /gene="dadA"
                     /locus_tag="OO332_001918"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5942414.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11589..12659
                     /gene="dadX"
                     /locus_tag="OO332_001919"
     CDS             11589..12659
                     /gene="dadX"
                     /locus_tag="OO332_001919"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5942415.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12827..14253)
                     /locus_tag="OO332_001920"
                     /pseudo
     CDS             complement(12827..14253)
                     /locus_tag="OO332_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14863..16599)
                     /locus_tag="OO332_001921"
     CDS             complement(14863..16599)
                     /locus_tag="OO332_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5942416.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16694..17608)
                     /gene="ldcA"
                     /locus_tag="OO332_001922"
     CDS             complement(16694..17608)
                     /gene="ldcA"
                     /locus_tag="OO332_001922"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5942417.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17708..18319
                     /gene="emtA"
                     /locus_tag="OO332_001923"
     CDS             17708..18319
                     /gene="emtA"
                     /locus_tag="OO332_001923"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5942418.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18321..19055)
                     /locus_tag="OO332_001924"
     CDS             complement(18321..19055)
                     /locus_tag="OO332_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5942419.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19256..19510
                     /locus_tag="OO332_001925"
     CDS             19256..19510
                     /locus_tag="OO332_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5942420.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19560..21020)
                     /locus_tag="OO332_001926"
     CDS             complement(19560..21020)
                     /locus_tag="OO332_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5942421.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20957..21514)
                     /locus_tag="OO332_001927"
     CDS             complement(20957..21514)
                     /locus_tag="OO332_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5942422.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21540..22394)
                     /locus_tag="OO332_001928"
     CDS             complement(21540..22394)
                     /locus_tag="OO332_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5942423.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22394..23203)
                     /locus_tag="OO332_001929"
     CDS             complement(22394..23203)
                     /locus_tag="OO332_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5942424.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23213..23971)
                     /locus_tag="OO332_001930"
     CDS             complement(23213..23971)
                     /locus_tag="OO332_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5942425.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23968..24948)
                     /locus_tag="OO332_001931"
     CDS             complement(23968..24948)
                     /locus_tag="OO332_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5942426.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24948..25970)
                     /locus_tag="OO332_001932"
     CDS             complement(24948..25970)
                     /locus_tag="OO332_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5942427.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6471 a         6395 c         6752 g         6362 t
ORIGIN      
        1 gcacagttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
       61 ctgtgtccat attacgtggg taggatcact atggcctttg ctgaactcaa cattgacatt
      121 cttacccgca gcaaagctcg tggaggttag cagtgcaagc agcaatacag ccttactgat
      181 gctcttgatt ttcattggta gtctcctgtt cagaatgagt acgatgaact gttataatat
      241 aacaatccct tacgggagaa ttccagtggc ttttctgcgt tcggtaaaat cccggcgcgg
      301 cattttttgc cggggggatt acttataaag gggcatggac tcaacaaacc gcagaaatct
      361 acagctggct ggcttgtggc gcgggtttca ttgatgttag cttatgccaa aacctggcat
      421 cttaaagagt aaaagtatgt tttgtgtgat ttatcgaagc agcaagcgtg accagaccta
      481 tttatatgtc gaaaaaaaag acgatttttc gcgtgttcct gaggaactga tgaaaggttt
      541 tggtcagcct cagttagcga tgattctgcc gttggatggg cggaagaaac tggtcaatgc
      601 tgatattgaa aaggttaaac tggcattaac cgagcaaggt tactatttgc agttaccgcc
      661 accacccgaa gatttgctga agcaacatct ttccgtcatg gggcagaaaa cagacgacac
      721 taacaaataa ccgatatccg gcggtggcat tatctttgtc ggcgcgggtt ttcatatcca
      781 cgataaggtg aggggaacgt tatgtatcaa catcacaact ggcaaggtgc gctgctggat
      841 tatccggtga gtaaagtagt ctgtgttggc agtaactatg ccaaacatat taaagagatg
      901 ggcagcgcag tgcccgaaga gccagtgctg tttattaaac cagaaacggc actgtgcgat
      961 ctgcggcagc cattggcgat cccatccgat ttcggttcag ttcatcatga agtcgaactg
     1021 gcggtgttga ttggcgcgac gctgcgtcag gcaacggaag agcatgtccg caaagccatt
     1081 gccggttacg gcgtggcgct tgatctgaca ttgcgtgatg ttcagggaaa aatgaagaaa
     1141 gccgggcagc cgtgggaaaa ggctaaagcg tttgataact cttgtccgct ttccgggttt
     1201 atccccgcgg cggaatttac cggcgatcca caaaatacaa cgctgggcct gagcgtaaac
     1261 ggcgaaaaac gccagcaagg tacgactgcg gacatgatcc ataaaatcgt tccgttgatc
     1321 gcttatatga gcaagttttt taccctcaag gccggtgacg ttgtgttgac aggcacgcct
     1381 gatggcgtcg gcccgttgca aagcggtgat gagctgacag tcactttcga tgggcattct
     1441 ttgacaactc gcgttttgta atactttttg ccgccagaaa gcggcggcaa aacttgcatc
     1501 gctgtgccag actggttata aggtgcgttt taacgtaatg gcggaacacc tgatgagcga
     1561 tgtacctttc tggcaaagta aaaccctgga cgaaatgagc gatgcggaat gggagtcgtt
     1621 gtgtgatggt tgcggtcagt gttgcctgca taaactgatg gatgaagaca ccgacgaaat
     1681 ctacttcact aacgtcgcct gtcgccagct caatattaaa acctgtcaat gtcggaacta
     1741 cgaacgtcgt ttcgagtttg aacccgactg cattaaatta acccgtgaaa atctgccaac
     1801 attcgaatgg ctgccaatga cctgcgctta tcggttgctg gcggaaggta aagatttacc
     1861 tgcgtggcat ccgctactta ctggttcgaa agcggcaatg catggtgaac gtatctctgt
     1921 gcgtcatatc gcagtgaaag aatcagaagt gattgactgg caggatcata tcttaaataa
     1981 acctgactgg gcgcagtgat aaatttaata gatgctgatg ggggctttct taatatacgt
     2041 ctggatgctc gccatttcgt agccctgttt ttgaaagcgc tctgtcataa tgagagttac
     2101 ccgatacctt attatcaaca ggtaactctc aatctttaaa aaacgtctgt gtttttaaat
     2161 gtgataaacc ttataccttg agtacatgga gagaataatc gcttatcaga cttcaggtac
     2221 ttcaaagagt gtctttttac cgtgtctttt ctgatactca ttacaggtgt taatcatttt
     2281 tttggccgct tcttttagca aagaaagcat taaatcatca taatcaacat agaatctggt
     2341 tgtttcagtt tccgttttta tctccccgat ggcggctatt tcggtggtta atttcaattt
     2401 ggcggcatcg atatctttat tcgcttgttt aaccgtgtta gacagggtgg taaagaaact
     2461 ctgcacagat tttaacttgt tcttcaattc tggaatcagt ttcccttcaa ctacgcccgc
     2521 agcaatagaa taggaaatga ttaaaccaaa tggaccggcg acgacatcgg ctgcggcacc
     2581 ggcatacgct tccttcctga ttttatctac ctgtgactgg aaatagctgc ttttttctga
     2641 aaaatcattg gttaactggc tatctaacgc cagcagtttc ccggaagcgt tgttgaaact
     2701 ttgtgagctt accagcaggg atttttgcgc ttcattcagc ttcgtgatgc cgtcatccag
     2761 taccttaatg aggggcggat gctttcttct cattgtactc atcaaatagc aaaatatacg
     2821 ctgcgagcaa ttgcgtcgca acaccacacc attcatacac tgtttgggtt gcttcaaaat
     2881 acttatcctg gctatccata agtaaggttt taatatcgcc gactaaaacg gaggctgcct
     2941 gtgaatactc ctgtttaaag cgacttaact cttttatggt ttcatcaaag gtctgccagg
     3001 ggatgacctg atcgagatat ttattataaa gatctaatgc tccatctgcg gtttcgattg
     3061 cgtttttaac tacttctacc gttttatctg caacgatttc agtcataatc attcgcctct
     3121 ttaaatatat aaattgtaat gaaactcctg ttttacaact attaataaat tttacttcat
     3181 ctaattcata gttagccggg cgggatgcgt caatgtcttt atttctatta atatgataaa
     3241 tatcaaacaa tgtttaatgt cattatggcg aatgcttcta ttctattttt ttgccgggtg
     3301 atatttttca tttctgctgg atgagcgtcg tcgccagaag gccacgtgag cacaggataa
     3361 gagaacgaaa aatcagcagt ctatgcagcg acaaatattg atagcctgaa tcagtattga
     3421 tctgctggca agaacagact actgtatata aaaacagtat agcttcaggc agattattat
     3481 gttgtttatc aagcctgcgg atctccgcga aattgtgact tttccgctat ttagcgatct
     3541 tgttcagtgt ggctttcctt caccggcagc agattacgtt gaacagcgca tcgatctgaa
     3601 tcaacggttg atccagcatc ccagcgcgac ttacttcgtc aaagcaagtg gtgattctat
     3661 gattgatggt ggaattagtg acggtgattt actgattgtc gatagcgcta ttaccgccag
     3721 ccatggtgat attgtcatcg ctgctgttga cggcgagttt acggtgaaaa aattgcaact
     3781 acgcccgacg gtacagctta ttcccatgaa cagtgcgtac tcgcccatta ccatcagtag
     3841 cgaagatacg ctggatgtct ttggtgtggt gatccacgtc gttaaggcga tgcgctgatg
     3901 tttgccctct gtgatgtaaa cgcgttttat gccagctgtg agacggtgtt tcgccctgat
     3961 ttatggggta ccggtggttg tgctatcgaa taatgacggt tgcgttatcg cccgaaacgc
     4021 tgaggcaaag gcgctgggcg ttaaaatggg cgatccctgg ttcaaacaaa aagatctgtt
     4081 tcgtcgctgt ggtgtggttt gctttagcag caattatgag ctttacgcag acatgagcaa
     4141 tcgggtgatg tcgacgctgg aagaactatc gccccgcgtc gagatttaca gtattgatga
     4201 agcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat ctgactgatt ttggcagaga
     4261 aattcgcgca acggtgctac aacgtaccca tcttactgtt ggtgtgggga tcgcccagac
     4321 caaaacgttg gctaagcttg ccaatcatgc ggcaaaaaaa tggcagcggc agacgggtgg
     4381 ggtggtggat ttatcaaatc tggaacgcca gcgtaaatta atgtctgctc tccccgtgga
     4441 tgacgtctgg gggattggac ggcggatcag caaaaaactg gacgcgatgg ggatcaaaac
     4501 cgttctcgat ttggcggata cagatatccg gtttatccgt aaacatttta atgtcatgct
     4561 cgaaagaacg gtgcgtgaac tgcgcggcga accctgtttg caactggaag agtttgcacc
     4621 gacgaagcag gaaattatct gttcccgctc gtttggtgaa cgcatcacgg attatacgtc
     4681 gatgcggcag gccatttgta gttacgctgc ccgggcggcg gaaaaacttc gcagtgagca
     4741 tcaatattgt cggtttatct ccacatttat taagacgtca ccatttgcgc tcaatgaacc
     4801 ttattacggc aatagcacgt cggtaaaact gctgacgccc actcaggaca gcagggatat
     4861 cattaacgct gctacgcgat ctctggatgc catctggcaa gcgggccatc gctaccaaaa
     4921 agcgggcgtg atgctggggg atttcttcag tcagggagtc gcgcagctca atttattcga
     4981 tgacaatgca ccgcgccccg ggagtgagca attgatggcg gtaatggata ctctgaatgc
     5041 taaagagggc agaggaacac tctattttgc cgggcagggg atccaacagc aatggcagat
     5101 gaagcgagct atgctttcac cacgttatac aacgcgaagt tctgatttac tgcgggtcaa
     5161 ataaatatag cggcaggaaa aaagcgttcc cgcaggagcg ccgaatggat tagcgaccga
     5221 acagatcacg ttttttcgct ttaaacggct gggaaatcac caccagcacc gcgacaatca
     5281 ggtaagcgat aaaaataccg agcagccact gcggcatttc catacctaaa aaatcccact
     5341 gacgctcggc gcaatcgcca gaggcgacaa acacttgcgg cacccattta tccagcggca
     5401 gccattccgg gaaacgaacc ataaaatcac aggtggcaaa tggcgaagga tagagctgaa
     5461 gcatggtgtg ctcgtaagtt aactgcacac cgcggaacgc actatacaac cagataacca
     5521 tcgctacata acgcagcgga gttttcgggg cgatcgcgcc aatcagcgca gcacccagaa
     5581 cgccgaataa cgcgcagcgt tcataaatac agagcacgca aggtttcagt aacatcacat
     5641 gctggaacca cagcgccgtc agttccagtg ccagagcagt aaacgccatc aacagccacg
     5701 cgccccggcc ttgtgaacat tggttcaaaa atcgcaacat aatcatttcc ctgcaatatg
     5761 catagagtgc gcagtttaaa ccaattcatt cgctgcgcca ccaggaggca gcaaaaatag
     5821 gacgcaattg gacaattatc gggcgaaaag gcaaaccggg catttatatg cccggtaagt
     5881 tgttatcaaa gcgttgctat ccagcccatt tgcataaacc attcggttac aggggcaagc
     5941 gtaaactcga cgcagagcaa gccgacgagt gtcagtacga gggtgtaagg cagggccatc
     6001 cacaccatgc ggccataaga gaggcgaatc aatggcgcga gtgcagaggt cagcaggaac
     6061 aggaacgcag cctgaccgtt cggcgtagcg acagagggca gattggtacc ggtattaatg
     6121 gcgaccgcca gcagctcgta ttgcttcaac gtgatagcgc cactttccat tgccgctttc
     6181 gcttcgttga tataaatcgt ccccacgaag acgttatccg aaatggatga cagcagaccg
     6241 ttgaaaatat agaacagcga cagctgagca tgttcagatg cctgcaacac aaactgaata
     6301 atcggcgaaa acagttgttg gtcgataatc accgcgacta ccgagaaaaa caccgtcaac
     6361 agtgcggtga atggcagaga ttcggtgaag gctttaccga tagcatgttc atcggtgaca
     6421 ccggtcaatg atgttgccag aataatgact gacaaaccaa tcaagccaac ttccgccaga
     6481 tgcaacgcca gcgcagtcac cagccagacg ccaataatcg cctggacaat cagacggatt
     6541 ttatcctgac gggtacgctg gtggcggctt tgatcgtcaa actgttgcaa cacttcgcgg
     6601 actttctccg gcagcgtttc accgtagcca aaccaacgca gcttctctac cagcaggcag
     6661 gttaacaggc cacaaatcag aaccggaacg gtcaccggcg acatgcgcag gaagaaatcg
     6721 ccaaaatgcc agccagccgc tttagcgatg atcaggttct gtggttcgcc caccatggtc
     6781 attacgccgc ctaatgcggt gccgacaccg gcatgcatca tcaggctacg cagaaagcca
     6841 cggaactgtt ccagaaccac gttgtaatgc ttgtcgatat gactatcgtc ttgcaggtcg
     6901 gtgtcttcgg tacgggaaga ggctacgcga tgataaatac cataaaaacc gactgcaacg
     6961 ctgatcacca ccgccacgac ggttaaggca tcgaggaacg cggagaggaa cgctgccgcc
     7021 acgcaaaaag agagcgacag cagcattttg gagcgaatgc ttaacagcaa acgggtaaat
     7081 atgaacagca acagctgttt cataaaatag atacccgcca ccataaacat caataacagc
     7141 aagacttcaa gatttgccgc caccccttca cggacgtgtt ccgcgctggt catgccgatg
     7201 aataccgctt cgatagccaa cagaccaccg gggagtagcg ggtagcattt cagggccatc
     7261 gccagagtga aaataaattc cgctaccagc aaccagcccg cgacgaaagg gctgatgagg
     7321 aaaattaacg ggtttacgat taagaaaatg atgagggcga gtttgtacca gtcgggggac
     7381 tggcccaaaa aattgcgcca tagcgcgcgg ccccaggaga tctccatgat ggtttccctt
     7441 accttacaaa taatcaatga tgtttttgtg tttaaacgca aagcttaacg gtcaggcagg
     7501 agtgaggcaa gtcttgatag tcaaggggaa agagatgcgg aaaatgaagc cttgatccct
     7561 ttttcttctt tttgtctgct atcagcgtag ttagccctct ggtatgatga gtccaacttt
     7621 gttttgctgt gttatggaaa tctcactatg gtcattaagg cgcaaagccc ggcgggtttc
     7681 gcggaagagt acattattga aagtatctgg aataaccgct tccctcccgg gactattttg
     7741 cccgcagaac gtgaactttc agaattaatt ggcgtaacgc gtactacgtt acgtgaagtg
     7801 ttacagcgtc tggcacgaga tggctggttg accattcaac atggcaagcc gacgaaggtg
     7861 aataatttct gggaaacttc cggtttaaat atccttgaaa cactggcgcg actggatcac
     7921 gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc gtaccaatat ttccactatt
     7981 tttattcgca ccgcgtttcg tcagcatccc gataaagcgc aggaagtgct ggctaccgct
     8041 aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg attacaacat attccgcggc
     8101 ctggcgtttg cttccggcaa cccgatttac ggtctgatcc ttaacgggat gaaagggctg
     8161 tatacgcgca ttggtcgtca ctatttcgcc aatccggaag cgcgcagtct ggcgctgggc
     8221 ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc acgatcaggt gtacgaaaca
     8281 gtgcgtcgct atgggcatga gagtggcgag atttggcacc ggatgcagaa aaatctgccg
     8341 ggtgatttag ccattcaggg gcgataatcc cttccgttta aagagcaaac ccctcaaacg
     8401 aggggttttt tgttgttttt acagatttcc cattcttggc gggcaacgtt ccagcaactc
     8461 gatgctgccg tcttcgtttt gctgttcgag catcacatca aatccccaca ggcgatgcac
     8521 atgcttcagg acttctttgc gcccccgatc cagcggtgcg cgattatgtg gaatataacg
     8581 cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag atctgaatat tcggctccag
     8641 attacttaag ttatattgcg acgataaccg gttacggatc tcccgataac cttcttcatt
     8701 atgaatagcg gaaatctcca gataattatg ccgatcatca tccagcacgg tgaagaagcg
     8761 gaaatcacgc atcactttcg gtgacagaaa ctggctgata aaactctcat ctttgaaatc
     8821 acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg gcgatatccg ggaaccagta
     8881 tttgtcttct tccgttggcg actgacaaat ccgtttaata tcctggaaca tggcgaaccc
     8941 gagggcatac gggttgatgc cgctgtacca cgggctgtta taggggggct ggaagaccac
     9001 attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc gttactttcc cttcatcata
     9061 cagatggtta aggatggtat agtgccagaa ggtcgcacag ccttcgttca taacctgagt
     9121 ctgtttttgc ggataaaaat actggctcac cttacgcaca atacgcagga tttcacgctg
     9181 ccatgattcc agcagtggcg catttttctc cataaaatag agcaggtttt cttgtggttc
     9241 ggacggatag cggcgcgctt cagcaaccgt tttctcttcc tcgcgcttcg gcagggtacg
     9301 ccagagcata ttgacctgac tttgtagata ctcttcgcga cttttctgcc gggctttctc
     9361 ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc acgccgtagt tcatcagcgc
     9421 gtggcacgag tccagaagcc gttctacttc atcaacgcca taacgttctt cgcactcggt
     9481 aatatattta cgggcaaaaa tcagataatc gacaatcgaa ctggcgtcgg tccaactgcg
     9541 gaataagcaa ttgtttttaa agaaagagtt atgcccatag caggtgtgtg ccatcaccag
     9601 cgcctgcata gtaatggtgt tctcttccat caagtaagcg atacacgggt tagagttaat
     9661 gacgatttca taggccagcc cttgctgacc gtgcttatac agccgttcag tctcgataaa
     9721 ctttttaccg aatgaccagt gcggatagtt aattggcatg ccgacgctgg aataggcatc
     9781 catcatctgt tctgaggtta tcacttcaat ctggtgcggg taagtatcca gccggtagag
     9841 tttcgccacc cggtctatct ctgccagata aacatccagc aggtcgaacg tccagtcggg
     9901 tccatcgctc aaacgtgtgg tgtccttatt aatagaatcg atcgtcgcca tacgcgcacc
     9961 tcattgttgt cggcgctctc tgtgtggagc acctcatttc aagcatagaa cacctgttaa
    10021 aaaccgcgtc gccggagaat ttttttcttt gcgatttctt attatcagag tgccactaat
    10081 ccgcttctga acggaatttt atgctggata aaaagggcgt tcagcaggag atactaaaga
    10141 cgccatattg ccgcagagtc agggagatgt gagtcagctc accataaaaa agccgtatgt
    10201 tgaataatat tttcaactga gttatcaaga tgtaattaga ttattattct tttactgtat
    10261 ctaccgttat cggagtggct atgcgagttg tcatactggg aagtggtgtg gtaggcgttg
    10321 ccagcgcctg gtacttaaat caggcaggac atgaggtcac cgtcattgat cgggagccgg
    10381 gggccgccct ggaaaccagt gccgcgaatg ccgggcaaat ctcccccgga tatgctgcac
    10441 cgtgggcagc accaggtgtg cctttaaaag cgattaaatg gatgttccag cgccatgcgc
    10501 cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa atggatgtgg caaatgttac
    10561 gtaactgcga caccagccac tacatggaaa acaaagggcg gatggtgcgt ctggcggaat
    10621 acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa tattcagtat gaagggcgtc
    10681 agggtgggac gctgcaactg ttccgtaccg aacaacagta tgaaaatgcg acccgcgata
    10741 tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct ggaatccagc cgcctggcgg
    10801 aagtggatcc cgcgctggca gaagtagcgc acaaactgac gggcggcctg cagttaccca
    10861 atgatgaaac cggagactgc cagctgttta cccagaatct ggcgcggatg gcagagcagg
    10921 cgggggttaa attccgcttt aatacgcccg ttgaccaact gctttgcgac ggcgagcaaa
    10981 tctacggcgt gaagtttggc gatgaagtga ttaaggccga tgcgtatgtg atggcgtttg
    11041 gttcttactc gacggcgatg ctcaaaggca ttgttgatat tccggtgtat ccgctgaaag
    11101 gctactcgct gactattcca attgcgcagg aagatggtgc gccagtatcc accattcttg
    11161 atgaaaccta caaaatcgcc attacccgtt tcgataaccg cattcgtgtt ggcggaatgg
    11221 cggaaattgt tggttttaac accgagctgt tgcaaccgcg tcgtgaaacg ctggaaatgg
    11281 tggttcgcga tctctatcca cgcggcggtc atgtcgagca ggcgactttc tggacgggtt
    11341 tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg tacacgcttt aaaaatctgt
    11401 ggctgaatac cggtcacggc acgctcggct ggacgatggc ttgcggttcc ggtcagttgt
    11461 taagcgatct gctctctggt cgcacgccag cgatcccata tgaggatcta agcgtagcgc
    11521 gctacagccg tggatttacg ccatcacgtc cgggccattt acatggcgca cacagctaag
    11581 gaaacgagat gacccgtccg atacaggcca gcctcgatct gcaggcatta aaacagaatc
    11641 tgtccattgt ccgccaggcc gcgccgcacg cgcgcgtctg gtcggtggta aaagcgaacg
    11701 cttacgggca tggtattgag cgtatctgga gcgcgctcgg ggccaccgat ggctttgcat
    11761 tacttaacct ggaagaggca ataacgttac gtgagcgcgg ctggaagggg ccgatcctga
    11821 tgctggaagg atttttccat gctcaggatc tggagattta tgaccagcac cgcctgacca
    11881 cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa tgcgcggcta aaagcaccgt
    11941 tggatattta tcttaaagtg aacagtggga tgaatcggtt gggcttccag cccgatcgcg
    12001 tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt tggcgaaatg accctgatgt
    12061 cgcattttgc tgaagcggaa catcctgatg gaatttccgg cgcgatggcg cgtattgagc
    12121 aggcggcgga ggggctggag tgtcggcgtt cgttgtccaa ttcggcggcg actctgtggc
    12181 acccggaagc gcattttgac tgggttcggc ctggcattat tttgtatggc gcttcgccgt
    12241 ccggtcagtg gcgtgatatc gccaataccg gattacgtcc ggtgatgacg ctaagcagtg
    12301 agattattgg tgtccagacg ctaaaagcgg gcgagcgtgt gggctacggc ggtcgctata
    12361 ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg atacgccgac ggttatccgc
    12421 gccatgcgcc taccggtact cctgttttag tggacggcgt gcgcaccatg acggtgggga
    12481 ccgtctcgat ggatatgctg gcggttgatt taacgccttg cccgcaggcg gggattggta
    12541 cgccggttga gctgtggggc aaggagatca aaattgatga tgtcgccgcc gctgccggaa
    12601 cggtgggcta tgagttgatg tgcgcgctgg cgttacgcgt cccggttgtg acagtgtaac
    12661 ttgttgtaag ccggatcgga ggcagcgtct tctggttgcg aaaaaatcat ccatccggct
    12721 ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg tccattaaaa
    12781 gaatggacac tatttccccg gaacctgaac tcgccgcaca ggcgttctac ataaaacgct
    12841 tacgcttcat tgttgactcg gagtcgactt agataaatca cgcttcaccc ttgatgcaga
    12901 ttcgggttca tgttcagatt gttgattaac aggctgcgag tcctgttgtc tttccagact
    12961 caacaggccc aactgttgat ccaggagctc caggtcctcc tgtaccttca cgcgctccag
    13021 gagttccggg tcatccagga actccagatc cataaggtcc tccagctcct cgagtccttc
    13081 caggtcaacc tagccttcct tgtgcaaaaa gtccaaaagg tccttcaggc cctgctgttt
    13141 tttcaggccc tcgacggctt tgagcagcgt cggtaaattg tctttgtccc acgggttacc
    13201 attacagtca aacaccttca ggctctgggg ctgcggcacc tgtaccgttt taaacttgtt
    13261 ttctccacag tagtaaatta ttagtttggg cggcagccgt ggtgaagaga tcaaatcgtt
    13321 actagaacag tcaaatactt tcaggcgagt caagttctcc agcatctcat caggtattga
    13381 gttaatattg ttgccggcac aggatagttt atacaggtac tggggcatcg acggcggtaa
    13441 aaattgcaag aagttttcag aacagttaat cactctcagg ttagtcgaca gctcaggcag
    13501 ccagataata cagttttcat agcacttcag taccatcagt tttgagggct gcaccttcgg
    13561 taacaagatc aagacgttat ctgtacagtc aagcaccagc agccactttg gcatcacctt
    13621 cggtaacgcg gtcaacacgt ttttactgca gtaaaattca atcagatgtg gaggcaaatt
    13681 ctcagggagt gaagtcaatc ccaggttgct aagattgagc atactccgtc ttgtaatgag
    13741 acattccttc attctgcgca atgcttctgc ccggttttca atccggcttg ctggcaggtt
    13801 ttcttccagg atattttcct tgatttcttc cagactttct ttcaggtctt cttggggaat
    13861 ttctgactgt ttgttttgct ggttttcttc caggatttcg tacaggtttt tttgcaggtt
    13921 ttcttgcaaa ccatcctgct gtatagccca ttcgcaccat ttggcgacca tcatgaactc
    13981 gttatgttgc tgattcaggc gagtccccaa cgtgatgatg gtattgttgt ccacctgtat
    14041 gttcactttg cccgcgatac tgaggtcaat gatctgccag ggggcatcac cccagctgaa
    14101 tttttgcggg ctgagatact tcatgttctg caaaatttga ttcttgagtt ccaccgacag
    14161 gaagagccgc cctggcgggc gtcgtacgtc tgggttaacg gaagatgggg gaatggcggg
    14221 ggcgaatggg cagatgtctt taaagctagc cataatgcta cgtttccttg aaataaatat
    14281 cctctggcag cgccggagac gtgaatgtgg tcaccgcaaa ggagccacag gacgtcattt
    14341 gcgctgatat cactactcat cgttgacttt ttccctggtc agaaatgccg tgtcgggtct
    14401 ggtacatgct gtattacggc ctaggtagct ctgacgtatt aaagaaaata cgagagtgaa
    14461 cgacccggaa ataatcgtta ttaagcaaag gaataactat aagaaatcgc tcatgcatcc
    14521 ggtgctacag gcggatgaaa cgcctattgt gtgggctatg tcagtgcggc aggttgcgtg
    14581 gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg gttgaccggt aatgcggggc
    14641 tggtttttat tacctgttac agtcggaaaa agcccttacg ctgactttct tgagccggtg
    14701 ttttgagttc attagccaga gcctgcaatg gtttgtcatc cataatttaa ccttcttctg
    14761 atgttgggat gagcatatca aaatcaggaa atgacaagtt tattacaggc tcaggctgcg
    14821 ttcagtaaag catcgcatcc ggcgatagat gctgcattaa atttaagatt cagcttcctc
    14881 ttcagccacc cgaacgccaa tcttcaacac ttcattatct tctttctcgg ccaccgtcca
    14941 gatcatcccg gcaaactcta cctggtcacc gacaaccggt gccgcgccta gcaactgctg
    15001 gacaatttca cccagcgttt gctgtttatc acgatattct cgcccgtctt ccaggccata
    15061 tatcagcgca acatcagcat atttggcgct ggcttcgaga atgaagtcac caaagaagcg
    15121 ttgatccagc gcgaccggtg gcgactggct gaacagttta ccgagcgccg ggagatcgcg
    15181 ttcccgacca attacacaca atacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg
    15241 aagcaactgg ttatcacgaa acagggctgc aatacgcgtc tcttttggca tatgcaaatc
    15301 gcgcagtgcc gcacctacac accatttatc agcactcaat tgataaacaa actgctccca
    15361 cggattttcc ggatgaatat ccaggccaac gcgtgacacc ggacgtccga cgggcggaac
    15421 caccactttg gcttttttcg ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac
    15481 cagaaccaca aagaaggcga cattaaagaa cagtcgcgca ttctccagcc ccgccatcat
    15541 cgggaacacg gccaggatga ttggcaccgc gccgcgtaat cccacccagc tgataaacac
    15601 gcgctcacgc agattgaaac cgcggaaagg gagcaatcca gcaaataccg aaaggggacg
    15661 ggcgaagaat atcatccatg ccgacaaaat gagcgccgga atggcaatag gcagcagatc
    15721 gcttgggtta accaatagcc ccagcaccag gaacatggcg atttgcgcca gccaggcgag
    15781 gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa
    15841 accgcacaga taaacagcca gaataccgct gccttccagc gccgtggtta aagcaaaaat
    15901 taaaataccg ccgcttaatg ccagcaatgg atataatccg gcaggcaggg cgatgcgatt
    15961 aatcatttgc agcagtaaat aaccaccgcc aagaccgatg acaatcccga ggccaaattg
    16021 ctgcagaata tcgaccacga acatccaact gacactgctt tcatgctgct ggatcattgc
    16081 aatcagggta atcgtcagaa agaccgccat tggatcatta ctgccggatt caatttccag
    16141 cgtcgagcca acacgttcgt taagcccctt accacccagc agagaaaaga ccgctgcagc
    16201 atcggtggag ccgacgatag cgccgattaa taagccttca atcaaatcaa gattaaacag
    16261 ccacgccgcc atcatgccgg ttaaaccaga ggtgataagt acgcccagcg tcgccagcga
    16321 cagcgccggt cctaacgcca cacgaaagga actggcctga gtgcgcatcc cgccgtcgag
    16381 caaaataatc gccagcgcca ggttacttac catgtaggcg aaggggtaat tatcaaacgg
    16441 gataccgccg acgccatcga ctcccgccag catgccgatc gccagaaaaa taaccagaat
    16501 aggaatgcca agacgggaag aaaatgaact aagtaatata ctgctggtga caaggatgga
    16561 tcctaagatg aaaaggctaa ttattgttgt ggcatccaac ggtcggttac tcctgattac
    16621 gctgtctctt atataaaccc taccatatta gcggcagaga cagcgtttta cttagccctg
    16681 aagcgtgatt tttttacatt ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt
    16741 cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat
    16801 gaccaaaatc gaggccggta atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca
    16861 ctgactccag gttgtaacct gcgtcataat cattgggcgt gctgccgcta aagctaccga
    16921 gaataatcgc cttctgacgt ggcaaaattc ccgcatgata gagctgcaac agcatacgtt
    16981 cgacgcggaa agggtgctcg ttaatatctt ccagcaccag aataccgttc tcaatttttg
    17041 gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag attgcctccc cacaacgtgc
    17101 cttcggtctg acatgtcggg ccttcccctt gccattcaat ggtgaaggtt tcattgcgta
    17161 acgccagcca gaaatggtgc tcggtaaagg cgttcagttc atccgcacca aagtttgcta
    17221 ccagcatggg gccgctaaag gtgatgacat tgccttgcgc cagaagaccg cactgaatgg
    17281 cggtaaaatc gctatgtccg caaatgagta acgggtcatg ttgttggcga gccaccagtg
    17341 cctgccagtc aatatctgcc agtaaacgac tggcaccgta accgccgcgt acagccagca
    17401 cgatggtgtt gggggtagtc agtctggcaa gggaattgag atcttccaga cgctccgttt
    17461 ccgtacccgc aaaacgctca caacgacggg caataacctc gacgttattg acttgatgcc
    17521 ccgcgtcggt aaggcgctgg ataccacgca gcgcggcgtg ctgtttaatg ctgtaacccg
    17581 atggggcaat taagtgaaac agagacatgg caattccttg ctgacaacag aaacgaaatg
    17641 tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg cgattccagg ctataaggat
    17701 agaagaagtg aaattgagat ggtttgcctt tttgattgtg ttattagcgg gttgttcatc
    17761 aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca
    17821 gtggatgcca ataagccaga aagccggtgc agcctggggc gtcgatccac aattgatcac
    17881 ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg gtgagtaaat cgaatgccat
    17941 tggtttgatg cagttaaaag cttcaacctc cggacgtgat gtttatcgcc gtatgggctg
    18001 gagtggtgag ccgacgacca gcgagctgaa aaatccagag cgtaatattt caatgggggc
    18061 ggcttacctg aatattctgg aaaccggccc gctggcaggc attgaagatc cgaaggtact
    18121 gcaatatgcg ctggtggtgt catacgctaa cggggcaggt gcgctgctac ggactttctc
    18181 gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg gatgctgacg agttcctcga
    18241 tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc tatatctaca aacttgagca
    18301 ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc
    18361 tcgagagaga aaataatccg ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa
    18421 cggaagctca gacggggagt ggtgatggtt tcattcttgc catcaatcac tttgcgctcg
    18481 ctggtggaga ttaactgggc gtcaaagtga aaaacacccc attgccccat gttgacttca
    18541 atttgagcga agcgcatacc ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg
    18601 cccatgccgc ctaacgacaa atcatacaag cggaaacgta acgtactgtt atccgccagt
    18661 ttggtctggc aaaaataagg cggatggagt ggggcggaga tgcggaaata tcggcgtcgt
    18721 tgtacaaacc ataaggtggg aggcggtacg gtaataaatg ccggaagctg caagtattca
    18781 ctctgctgta gttgttcaac agtaaactcg actttcgcac cctgagtttc ggcggtaatg
    18841 gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg cttgactgcc gaaatccagc
    18901 accagtttat ccggggttat tgccagtatt ttgctgatca gctgcccgcc attccaactg
    18961 agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga
    19021 ttttgtttca ggaactgctc atggtaatga ctcacgcgga aaaactcctg actgacaaac
    19081 tgtctttggt ttatcggtca tagttaacaa aacttaatac aaatgcgtga atatttttta
    19141 catgttgttc ttaaatcagc cgacaaacgc ccagcatcga tcccgccctt gcctatactt
    19201 agagcgttga cgtaagcatt tcttgcgacc gattcatcga aacgagggcc tgaacatggg
    19261 aattattgcc tggattattt ttggcctgat agccggcatt atcgccaagc taatcatgcc
    19321 ggggcgtgat ggtggtggat ttttcctgac ctgtattctc gggatagtcg gtgcggtggt
    19381 cggcggctgg ctggcgacca tgtttggcat tggcggctcc atcagcggtt ttaatttgca
    19441 cagcttccta gtggcggtgg tgggcgctat cctggtactg ggcgtattcc gcctcctgcg
    19501 aagagaataa gattttcata aggcggatag cgatacagat gccgctatcc gctttcacat
    19561 cagaacgtat actcgacacc tgcccagaaa ttgcgacctt cttcaataaa cccttcactg
    19621 taggcatatt gggtatcaaa caggttattg accgacgcat tgacgctgaa gccatgacct
    19681 aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg cggctttttg tgaaccgtca
    19741 ctgttgctgt agctggagga acgcgcctct tccgacagcg ttacgcttaa cggctcccat
    19801 ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa gatcggttat cttgccgatg
    19861 tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca atcctacatc cagtatattg
    19921 ctgattttcc ccttaatacc ggcatccaga ccgctgtaat ccaccgtgcc gctgttctga
    19981 ttttgaatgg tatcggcatt gatattgtgc gagaggatgg catcactcac ccggttatag
    20041 taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac cattccaggt taaatccacc
    20101 ccgcgagcgc gttccggctt gagctgcggg ttaacaatcg ctatctggtt atacgcaggt
    20161 ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg tgcggtcata gtacgaaagc
    20221 gccagagtgt cttcattggc aaagtggtat ttccccatca cctgccagtt aaaagctgac
    20281 tgattgttgt cgtcataatg ggtgatgctg ccatctttct cgtgtttttt cgcttctacg
    20341 ctatcgcgcc agtcatagct gattcccgcc acgacatcga cattatcagc agcagcccat
    20401 tgatattcac tggcgagcga ccaggtacga tcttcatagc gatcgtaagc ggcgtgcggc
    20461 gcaccttttt cccggtgtac gtcatctttc cagttaacgg caaacgacag cagatcgttt
    20521 tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc tgtaatcgga ataatggctg
    20581 tagctgcctt ttttattttt caaatcagcc agcgagttgt acatcatcag cgtattttca
    20641 aaggtgtcgc gatacagccg acttttcagg gtaaaacggt cgtttagttg ggtcgctccc
    20701 tgataataaa aactttcttt gtcatactct ggccactgcc agtagcgtga tttttgacca
    20761 caatttccgc tgtatggcgg gttatctttt tcaccatcct gcttaatgta agtcagcgtg
    20821 tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa tgccgcgttt atcatcagcc
    20881 gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat tatttacacc atgcggcagg
    20941 ccgagaaaat cctgctttag ctggctaccg ctgacttgca aatacccaag atcgctgctg
    21001 gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac tacggctcca tccctggcga
    21061 tatcccagac ttgcttcttc agggtggtga tattaatggc tccgcccatc tgattaggcc
    21121 catgaagcag cgacgaatac cctttggaaa cttcgactgc ccccagattg ttggtcagaa
    21181 cccgcgccag atcgaggttg ccgtcatagg gaacataaat gggcacaccg tcgaaataga
    21241 ccggcacctg ccgactatca aagccacgaa cttttacctg ttcttcgttg cggctgcctg
    21301 acttttgcag caccacgccg gggacgacac ttaatgcctg agcgacattc tgtttatcca
    21361 aggctttcat ggtgggttga tcgagaacgg tggtcgtcgt cgatgatacc ggactggacc
    21421 atacggtcag cgtgtcgctc tcctgagccg ctacggcctg ctgggtaaag gcaagtgata
    21481 atgctgtgca gagataacgt tttttgagtc tcatagaata aattccgttt aacaaagaat
    21541 taaatagagg ctgtgggttg cagactgact ttaatgtcag ctggtgccgc gtaataaggc
    21601 gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat ttttgagtgt cgtcagatta
    21661 attccgccgg tcagagccag cgtgcagtga ggagccagcg acggcgctat ttgcgcaatt
    21721 tcagttgcct gttgcggact aaatttgtcg agctgaagta cgtctggttg tgcgcgtaac
    21781 gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa ttttcttctc gggtgcgtgg
    21841 cgacgtaact gagcgattgc gcctgaccag ccctgattgt catgaagaaa atggcggtgg
    21901 ttggcaaaca ataatatggt ttccgcacat ccggcacgat gaattagtcc tccggcagcc
    21961 agaattgcct gcgaagccag taagcgggtg cccggaattg cttttcgggt gcaggcgata
    22021 ttgccgtcag gataacgttc acgaagcaat gccagcattt gagcgagata atcagaaaca
    22081 ccgcaactcc actccagcac attctggact gccttccatc cctgatgaag tgctgcggca
    22141 ttaccctgcg cacggattag ccgttgaccc gcgttcgctt gtgaaccgtc gctgaccgcg
    22201 tcatcaatag ttaatcccag cgtagttaac atcttacacg cgacagaaac gccgctgacg
    22261 cagccgccct gacggtgaaa aaacgctata tagccaggct ggtgtccaat atttaatgct
    22321 cgcgtggtca ggtcacctcc ctggatatct tccagcagta acgcatcgat ttgtgcctga
    22381 gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc ccaccagatg agcgcccagt
    22441 cgcggctggc aggggcgagg gcacgagcat cttgttgttg ataccagtga tataaccgct
    22501 cacgctcatc gtcgttaagc gcgccaagac tccagctgac gttctgctca aaacgttccc
    22561 aggtgctgtt atcctgttgg caattttgtc cacggataaa atcaacgtgg gcataaattc
    22621 ccatctgata caggacgttg agcgcaaaga tatagttggg taattcgatc acttcccgac
    22681 cagccgcccg ctgaatggct ggagagacga acgaggtgct gaccagatga gttgtataga
    22741 cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg gcgcatatca gccaccagag
    22801 tggagcggga agccacggca atatcgcagc gtggcaggtc actccagtcc tcttcccagg
    22861 cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc ggcgcggcgg gcggcaacgt
    22921 tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt gagtttgtca gccagcgcca
    22981 gcgatacggt gcctggtccg cagcccatat cgaataaggt ctgagcgcct tgcaggtcaa
    23041 ttttagcgat cagttgttgc aggtagctgt ccgttggcga ggcgcaattt tccgccattt
    23101 tctccgcccg ttgatcccag tgatcgggcg ttttttctgt gcggtgagcc agtttgagtt
    23161 gctgcagata aaggtctgca aaatcaatat catcaatgag catgattgtt ccttagcggc
    23221 taattgcaga gaggtgatga tgaatttggt cggcagaaac ccgatacagg gccgccagtt
    23281 tgttcgttgt taactgttcg gtcggtaagc cttgtgttac tcgtccatct ggctctacct
    23341 ggataatgct gtcggcaatg gcgttggcat ggagcggatg gtgagtcgac attaacattg
    23401 tcatgccgtt atttttgagc tgcgccagcg tgtccagaag ttggatctga tgaccgaaat
    23461 cgaggctgga ggcgggctca tccagtaaca acaagcgcgg ttgttggacg agtgcacggg
    23521 cgattaaagc cagctgacgt tcaccgccgc taagcgtatt ccagcgtcgt gtcgccagat
    23581 gcaaaatgcc cagcttttcc agttgctccg tggctttcag gcgttcttgc ttaccgggaa
    23641 cggaaaaagc gcctatggtg ggggccaggc ccatcaacac catatccagc acactgaaag
    23701 caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc ctgtgcgcgc tggcgatggc
    23761 tgagtgtctg cacagggagg ctatccagta ggatctcgcc ttttaataac ggtataacgc
    23821 caagaatgga gcgcatgagt gtggttttgc cgcaaccatt cgcgccaagc agacaacaaa
    23881 ttgttccttg cgacagggtg aaggacacgt cgcgtagcac tggatggcgg gagtaaccca
    23941 gagacgcatg acgaacggca agcaaagtca ctggcgacct ccgcgtaaca acaaaagcag
    24001 gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt gggatttctg ttgttccaat
    24061 gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg ccagtacaca ttgccatagg
    24121 caacagctgc tgatggttat gaccggtcaa caagcgggca atgtgcggaa cgaccagacc
    24181 gatccaacca attatgcccg ctattgctac cgtgctggcg gtgattagtg tggcgcagac
    24241 aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc gaacgggctt cgtcgtcgga
    24301 aagcgtgagt aaattcatgc gccagcggag aaaaaacagg ggcaaggagc ctatgagaat
    24361 gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg gacagtcccc ccagcaacca
    24421 gaaggtaatt gacggtaact gggtgtaagg atcggccagc gttttaattg gcgatatccc
    24481 cgctccgcac aacgttccta acgcaatgcc taccagcacc agggttaaga tcggatcgtg
    24541 gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc accatcagct cgccgcaaaa
    24601 tgcgtacagc tggatgtaaa caatgggtaa tccccacagg attgccgcgc aggcaccaag
    24661 tccggcaccg gcggagacgc ctaaaatatc cggtgaaact aagggattac gcaacattcc
    24721 ctgataggtg gttccggctc ctgccagcgc tgcgcccagt aacagcgcag caagaatacg
    24781 cggcacgcgg atttgccaga agacaatttg ttcctgcaca gacacatttt cttgacgaag
    24841 aattagcgcc agcagttgct gcatatcgag atgataagcc ccacttacgg cggcaaaagt
    24901 gatactgata gctgccagca gcaaaataca tccgtttacg attctcatta gctcgccacc
    24961 aatttttgat agtcggcatc actgagtgaa caatgccaga acagttgggc gtaatgctgc
    25021 atgtcacttt taaactggcg attgatggcg ggatccagcc aggcgtgaag tctgcgtaat
    25081 cccagaagac ggttgattcc cggcggggca tcgagccagc caaagggcaa gccacttaaa
    25141 aataagatgc gttgttccgc aacggctttc acgccctgcc agagaggatc acgacgaata
    25201 aaatctgcag taacggcctc ctgaaccaga ataatatccg gctgccagcg caggagattt
    25261 tccatggaaa cctgtgtcag gccgtggcga tcggctattt gcgcgacgtt gtgcaaacct
    25321 aataattccg ccgcctcggt atgcaacgaa ccctgtaagc ccgtttccag ccctcgggga
    25381 ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg tggcgaatgc ctgagcttca
    25441 ccaacgaagt ggctggcgag attggcttgt tctgcggcgc ggtgctcttc gcctaacgtt
    25501 ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt ttccgttaag caataaccag
    25561 gggatttgtg tctgttcgct aacctgccgt gcctgggaga tcaaggtttc atccgtattg
    25621 ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc cttccagcga tagtgtgctg
    25681 gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa tgtgctctgg caatggaatt
    25741 aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca ttttttctgg cgcgacagcc
    25801 agcaatagca aatcggcggg cgcgcctgcg ctgacgatgc gtgtaatgtt ttgggcttcg
    25861 ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa aggacggtag agactgcagg
    25921 agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg ttatgggcat ggtgaacatc
//
LOCUS       DAJTRQ010000044        25448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000044 DAJTRQ010000000
VERSION     DAJTRQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            5..523
                     /locus_tag="OO332_001933"
     CDS             5..523
                     /locus_tag="OO332_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5942428.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
     gene            complement(533..1960)
                     /locus_tag="OO332_001934"
     CDS             complement(533..1960)
                     /locus_tag="OO332_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5942429.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1957..2664)
                     /locus_tag="OO332_001935"
     CDS             complement(1957..2664)
                     /locus_tag="OO332_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5942430.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2822..3805
                     /locus_tag="OO332_001936"
     CDS             2822..3805
                     /locus_tag="OO332_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5942431.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3875..4357)
                     /locus_tag="OO332_001937"
     CDS             complement(3875..4357)
                     /locus_tag="OO332_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5942432.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4592..7174
                     /gene="leuS"
                     /locus_tag="OO332_001938"
     CDS             4592..7174
                     /gene="leuS"
                     /locus_tag="OO332_001938"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5942433.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7189..7770
                     /gene="lptE"
                     /locus_tag="OO332_001939"
     CDS             7189..7770
                     /gene="lptE"
                     /locus_tag="OO332_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5942434.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7770..8801
                     /gene="holA"
                     /locus_tag="OO332_001940"
     CDS             7770..8801
                     /gene="holA"
                     /locus_tag="OO332_001940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5942435.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8803..9444
                     /gene="nadD"
                     /locus_tag="OO332_001941"
     CDS             8803..9444
                     /gene="nadD"
                     /locus_tag="OO332_001941"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5942436.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9468..10079
                     /locus_tag="OO332_001942"
     CDS             9468..10079
                     /locus_tag="OO332_001942"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5942437.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10339..10656
                     /gene="rsfS"
                     /locus_tag="OO332_001943"
                     /gene_synonym="rsfA"
     CDS             10339..10656
                     /gene="rsfS"
                     /locus_tag="OO332_001943"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5942438.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10660..11127
                     /gene="rlmH"
                     /locus_tag="OO332_001944"
     CDS             10660..11127
                     /gene="rlmH"
                     /locus_tag="OO332_001944"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5942439.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11158..13059
                     /gene="mrdA"
                     /locus_tag="OO332_001945"
     CDS             11158..13059
                     /gene="mrdA"
                     /locus_tag="OO332_001945"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5942440.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13062..14174
                     /gene="mrdB"
                     /locus_tag="OO332_001946"
                     /gene_synonym="rodA"
     CDS             13062..14174
                     /gene="mrdB"
                     /locus_tag="OO332_001946"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5942441.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14185..15273
                     /gene="rlpA"
                     /locus_tag="OO332_001947"
     CDS             14185..15273
                     /gene="rlpA"
                     /locus_tag="OO332_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5942442.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15412..16623
                     /gene="dacA"
                     /locus_tag="OO332_001948"
     CDS             15412..16623
                     /gene="dacA"
                     /locus_tag="OO332_001948"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5942443.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16734..16997
                     /locus_tag="OO332_001949"
     CDS             16734..16997
                     /locus_tag="OO332_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5942444.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17098..17739
                     /gene="lipB"
                     /locus_tag="OO332_001950"
     CDS             17098..17739
                     /gene="lipB"
                     /locus_tag="OO332_001950"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5942445.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17998..18951
                     /locus_tag="OO332_001951"
     CDS             17998..18951
                     /locus_tag="OO332_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5942446.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19160..20125
                     /gene="lipA"
                     /locus_tag="OO332_001952"
     CDS             19160..20125
                     /gene="lipA"
                     /locus_tag="OO332_001952"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5942447.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="OO332_001953"
     CDS             complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="OO332_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5942448.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20558..21346)
                     /locus_tag="OO332_001954"
     CDS             complement(20558..21346)
                     /locus_tag="OO332_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5942449.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21439..21822
                     /gene="crcB"
                     /locus_tag="OO332_001955"
     CDS             21439..21822
                     /gene="crcB"
                     /locus_tag="OO332_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5942450.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="OO332_001956"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="OO332_001956"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5942451.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22260..22820)
                     /gene="pagP"
                     /locus_tag="OO332_001957"
     CDS             complement(22260..22820)
                     /gene="pagP"
                     /locus_tag="OO332_001957"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5942452.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23409..24794
                     /gene="dcuC"
                     /locus_tag="OO332_001958"
     CDS             23409..24794
                     /gene="dcuC"
                     /locus_tag="OO332_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5942453.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24835..25176)
                     /locus_tag="OO332_001959"
                     /pseudo
     CDS             complement(24835..25176)
                     /locus_tag="OO332_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6516 a         6322 c         6249 g         6361 t
ORIGIN      
        1 ttttatagtt gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa
       61 agccatggtg aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac
      121 ggcagccacg gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga
      181 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      241 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
      301 gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc
      361 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg
      421 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      481 caaaaatgcg gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga
      541 agagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      601 tgacgcccag acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg
      661 gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      721 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
      781 cttctgtcgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg
      841 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      901 cgaccagtga acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa
      961 aacgcgtacg ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca
     1021 gatcacgcgc ttcaatcatc atcaccacta atgggtaata atcgacccga tgctgacggc
     1081 accattgcaa cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa
     1141 gttctgccag caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac
     1201 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg
     1261 ggattcccag gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1321 cggggataat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1381 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1441 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1501 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1561 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1621 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
     1681 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1741 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1801 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
     1861 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1921 tcgtttcctc tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag
     1981 cgtttccggg catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc
     2041 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     2101 cgctaagaat tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg
     2161 accaaaggta taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag
     2221 atgaatacgc gacaggatcc atagactttc ttcttcactc aaccagttat taagtacgcc
     2281 cattcgactt aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca
     2341 gcattcggct gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc
     2401 aagatagtca cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2461 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2521 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga
     2581 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2641 tattttttgc gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgcaa
     2701 actacatttc gagactcagt tatttatact tttctgacgc aacataatac gcataaaata
     2761 aggattacgc aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga
     2821 aatgataata atttttacgt caagttgctg cgataattta tcaatagatg agattatcga
     2881 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
     2941 tagcgcaatt gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga
     3001 gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg
     3061 tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac
     3121 tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga
     3181 agcggttacc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
     3241 tggatggatg tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg
     3301 gtacaaacaa gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta
     3361 taaagacgga gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag
     3421 cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga
     3481 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga
     3541 aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa
     3601 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
     3661 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
     3721 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
     3781 tcgtgagact gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc
     3841 catattctct ttattgctat gcgaaatcag actattacgg ctcaaacggg cgtctctgga
     3901 actggtcata accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
     3961 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
     4021 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
     4081 tgtctgccag ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt
     4141 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc
     4201 gcgtcagctc atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
     4261 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg
     4321 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
     4381 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
     4441 aaattaatgt acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
     4501 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
     4561 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
     4621 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
     4681 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
     4741 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg
     4801 caaaaacgtt ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
     4861 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
     4921 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
     4981 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
     5041 taaaaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
     5101 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
     5161 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
     5221 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
     5281 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
     5341 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
     5401 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
     5461 tgccgaagct gaaatggcga cgctggagaa aaaaggcgtc gatactggct ttaaagctgt
     5521 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
     5581 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
     5641 taaatacggc ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct
     5701 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct
     5761 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
     5821 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
     5881 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct
     5941 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
     6001 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
     6061 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
     6121 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
     6181 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
     6241 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
     6301 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
     6361 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
     6421 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
     6481 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
     6541 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
     6601 gaaacgtgtc tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa
     6661 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc
     6721 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
     6781 ggagctgatg aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat
     6841 gcaggaagca ctgctggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt
     6901 cacgctgtgg caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
     6961 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
     7021 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
     7081 ccaggaacat ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt
     7141 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
     7201 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
     7261 gacaccacgc aggttccttc cactatgaag gtcatgatcc tagactcagg cgatccgaac
     7321 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
     7381 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
     7441 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
     7501 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt
     7561 tcattcttcg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
     7621 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
     7681 gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct
     7741 gcacgcgtct ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg
     7801 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
     7861 tgcaggaaag ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc
     7921 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
     7981 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
     8041 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
     8101 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
     8161 cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg
     8221 ttgctgtgcg cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct
     8281 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc
     8341 tctggccaga cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
     8401 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
     8461 atattcttca gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac
     8521 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgta
     8581 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
     8641 atcgcttaag tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
     8701 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
     8761 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
     8821 ctgtttggcg gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg
     8881 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
     8941 ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
     9001 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgca
     9061 caaacactga aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt
     9121 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
     9181 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
     9241 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
     9301 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
     9361 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt
     9421 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
     9481 taattcgtca tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca
     9541 cacccctgac cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg
     9601 tttcctttga cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9661 ttctcagtga ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg
     9721 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9781 cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt
     9841 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt
     9901 taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg
     9961 ccgagtctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    10021 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    10081 ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat
    10141 tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg
    10201 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10261 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10321 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10381 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10441 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10501 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10561 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10621 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10681 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10741 ccgaaagata tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg
    10801 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10861 cgcattgtca tcctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10921 ctggaacgct ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg
    10981 ttgtcgcctg cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc
    11041 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    11101 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11161 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11221 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11281 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11341 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc
    11401 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    11461 ctggacgctt tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa
    11521 gagcgcgcac gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta
    11581 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    11641 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggata tgtgtcgaaa
    11701 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11761 acgcatgata ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11821 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11881 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11941 attgaaacgc tgctggcggg tagccgcgca gctgtggttg ttaccgatcc gcgtacaggt
    12001 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    12061 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    12121 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    12181 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    12241 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12301 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12361 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12421 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12481 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    12541 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12601 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12661 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12721 gttgctaacc gccctaatgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    12781 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12841 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12901 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12961 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    13021 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggct aatccgaata
    13081 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat
    13141 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13201 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13261 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13321 tggcggtaga tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta
    13381 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13441 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    13501 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13561 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13621 cagtagtgct ggtagcggcg ttcattccga ttctatggtt cttcctgatg catgattacc
    13681 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13741 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13801 acggcactca gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg
    13861 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc
    13921 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13981 ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    14041 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa
    14101 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14161 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14221 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14281 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14341 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14401 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14461 aacctgacgg cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg
    14521 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14581 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14641 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14701 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttat
    14761 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14821 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14881 ccggtaacca gtagcggttt cctcggcgct caaacgacct tagcgcctgg tgtactggaa
    14941 ggcagcgaac cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca
    15001 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    15061 gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15121 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15181 ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15241 caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15301 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15361 gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc
    15421 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    15481 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    15541 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    15601 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    15661 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    15721 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    15781 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    15841 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    15901 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    15961 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    16021 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    16081 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    16141 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    16201 ttgatttctg cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    16261 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    16321 ttcgcctcag aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa
    16381 gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    16441 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    16501 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    16561 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    16621 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    16681 actcccacct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    16741 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    16801 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    16861 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    16921 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    16981 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactctcctc
    17041 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    17101 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg
    17161 gctatgcatg aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc
    17221 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    17281 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    17341 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    17401 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    17461 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    17521 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    17581 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    17641 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    17701 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
    17761 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    17821 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
    17881 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    17941 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    18001 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    18061 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
    18121 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    18181 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    18241 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    18301 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    18361 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    18421 cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt
    18481 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    18541 catgttctgt tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct
    18601 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg
    18661 caaaatttca gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat
    18721 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    18781 attattccca gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct
    18841 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    18901 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    18961 gttacagcca gatcacagtg cagaacgata aaatggcaca atccaacaac aattttacat
    19021 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    19081 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    19141 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    19201 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    19261 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    19321 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    19381 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    19441 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    19501 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    19561 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac
    19621 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg
    19681 tcgaatggat cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa
    19741 cctggaaaac gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc
    19801 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    19861 gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    19921 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    19981 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    20041 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    20101 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    20161 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    20221 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    20281 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    20341 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    20401 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    20461 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    20521 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    20581 agcgacggtt gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    20641 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    20701 ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac
    20761 aggtggtatc cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc
    20821 cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg
    20881 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct
    20941 ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg
    21001 catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca
    21061 gcatattcca tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca
    21121 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    21181 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa
    21241 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    21301 tttcccacac agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc
    21361 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    21421 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    21481 ggaagcgtag cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    21541 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    21601 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    21661 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    21721 cgctttggct gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc
    21781 gcactggcat tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc
    21841 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21901 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21961 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    22021 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    22081 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca
    22141 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22201 ttttgatgtt gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgcct
    22261 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22321 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22381 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22441 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22501 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22561 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22621 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22681 tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct
    22741 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22801 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22861 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22921 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22981 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    23041 atgcaatcaa ggaaaaatta ttttattttt ttaacgagat acaacaatca tcttaacgaa
    23101 gtatataata tatatcgtca acccatgctc atattagatt attttttgag cgcacagccg
    23161 cagctaacac cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta
    23221 ttatgtgata attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa
    23281 cttcaatgtc ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg
    23341 ggcatatttt ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag
    23401 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23461 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23521 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23581 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23641 acctcggcat gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg
    23701 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23761 tgctgatgat tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa
    23821 ccggtctggg tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca
    23881 gtcgtggcgc agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23941 caggggatgt ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca
    24001 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    24061 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24121 tcaccaccac tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24181 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24241 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24301 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24361 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24421 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24481 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24541 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24601 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    24661 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    24721 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    24781 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24841 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24901 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc
    24961 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    25021 gtttagcgtc aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata
    25081 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25141 tttacactgg cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga
    25201 aaaaaccggg agaacattgg attattcggg atctgatggg attagatttg gtggggcttg
    25261 caagcctgta gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt
    25321 gcttttgttt cattgaaaaa acgaaaaaca aaaacactat aaatcatttc aatcatagct
    25381 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtcacc aaatgactca tatcacaaat
    25441 cagctcat
//
LOCUS       DAJTRQ010000045        25262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000045 DAJTRQ010000000
VERSION     DAJTRQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..815)
                     /locus_tag="OO332_001960"
     CDS             complement(<1..815)
                     /locus_tag="OO332_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HCR5942454.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGT"
     gene            complement(881..1261)
                     /locus_tag="OO332_001961"
     CDS             complement(881..1261)
                     /locus_tag="OO332_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942455.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            complement(1258..1568)
                     /locus_tag="OO332_001962"
                     /pseudo
     CDS             complement(1258..1568)
                     /locus_tag="OO332_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1844..2140)
                     /locus_tag="OO332_001963"
     CDS             complement(1844..2140)
                     /locus_tag="OO332_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942456.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            complement(2313..2987)
                     /locus_tag="OO332_001964"
     CDS             complement(2313..2987)
                     /locus_tag="OO332_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCR5942457.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3078..3278
                     /locus_tag="OO332_001965"
     CDS             3078..3278
                     /locus_tag="OO332_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5942458.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            3322..3873
                     /locus_tag="OO332_001966"
     CDS             3322..3873
                     /locus_tag="OO332_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942459.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            4049..4228
                     /locus_tag="OO332_001967"
     CDS             4049..4228
                     /locus_tag="OO332_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5942460.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            4218..5159
                     /locus_tag="OO332_001968"
     CDS             4218..5159
                     /locus_tag="OO332_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5942461.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            5156..5650
                     /locus_tag="OO332_001969"
     CDS             5156..5650
                     /locus_tag="OO332_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5942462.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            5650..6303
                     /locus_tag="OO332_001970"
     CDS             5650..6303
                     /locus_tag="OO332_001970"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR5942463.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6300..6626
                     /locus_tag="OO332_001971"
     CDS             6300..6626
                     /locus_tag="OO332_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5942464.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            6623..7012
                     /locus_tag="OO332_001972"
     CDS             6623..7012
                     /locus_tag="OO332_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5942465.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            7032..7829
                     /locus_tag="OO332_001973"
     CDS             7032..7829
                     /locus_tag="OO332_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5942466.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            7837..8826
                     /locus_tag="OO332_001974"
     CDS             7837..8826
                     /locus_tag="OO332_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5942467.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            8841..9209
                     /locus_tag="OO332_001975"
     CDS             8841..9209
                     /locus_tag="OO332_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR5942468.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(9238..10569)
                     /locus_tag="OO332_001976"
     CDS             complement(9238..10569)
                     /locus_tag="OO332_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCR5942469.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            10866..11192
                     /locus_tag="OO332_001977"
     CDS             10866..11192
                     /locus_tag="OO332_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5942470.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            11196..11672
                     /locus_tag="OO332_001978"
     CDS             11196..11672
                     /locus_tag="OO332_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5942471.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            11656..12048
                     /locus_tag="OO332_001979"
     CDS             11656..12048
                     /locus_tag="OO332_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR5942472.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            12511..12843
                     /locus_tag="OO332_001980"
     CDS             12511..12843
                     /locus_tag="OO332_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942473.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            12894..13244
                     /locus_tag="OO332_001981"
     CDS             12894..13244
                     /locus_tag="OO332_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5942474.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            13370..13864
                     /locus_tag="OO332_001982"
     CDS             13370..13864
                     /locus_tag="OO332_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5942475.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            14098..15594
                     /locus_tag="OO332_001983"
                     /pseudo
     CDS             14098..15594
                     /locus_tag="OO332_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            15606..15788
                     /locus_tag="OO332_001984"
     CDS             15606..15788
                     /locus_tag="OO332_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942476.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            15788..17029
                     /locus_tag="OO332_001985"
     CDS             15788..17029
                     /locus_tag="OO332_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5942477.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            17007..17657
                     /locus_tag="OO332_001986"
     CDS             17007..17657
                     /locus_tag="OO332_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5942478.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            17672..18877
                     /locus_tag="OO332_001987"
     CDS             17672..18877
                     /locus_tag="OO332_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5942479.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            18927..19127
                     /locus_tag="OO332_001988"
     CDS             18927..19127
                     /locus_tag="OO332_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942480.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            19130..19453
                     /locus_tag="OO332_001989"
     CDS             19130..19453
                     /locus_tag="OO332_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5942481.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            19450..19860
                     /locus_tag="OO332_001990"
     CDS             19450..19860
                     /locus_tag="OO332_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5942482.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            19835..20341
                     /locus_tag="OO332_001991"
     CDS             19835..20341
                     /locus_tag="OO332_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942483.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            20338..20898
                     /locus_tag="OO332_001992"
     CDS             20338..20898
                     /locus_tag="OO332_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942484.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            20907..21074
                     /locus_tag="OO332_001993"
     CDS             20907..21074
                     /locus_tag="OO332_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5942485.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            21061..22554
                     /locus_tag="OO332_001994"
     CDS             21061..22554
                     /locus_tag="OO332_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5942486.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            22554..22910
                     /locus_tag="OO332_001995"
     CDS             22554..22910
                     /locus_tag="OO332_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5942487.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            22910..23179
                     /locus_tag="OO332_001996"
     CDS             22910..23179
                     /locus_tag="OO332_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5942488.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            23321..25156
                     /locus_tag="OO332_001997"
     CDS             23321..25156
                     /locus_tag="OO332_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5942489.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
BASE COUNT         6269 a         5921 c         6956 g         6116 t
ORIGIN      
        1 aaagtaccaa taaaggtttc tactttgctg tccttgaatt tctcaacaag cagatcacga
       61 aattcgttag ccatttcttc ctgcaccgcc tccagctgaa taatgcgcag aaccagtacc
      121 ggacgatcgc cagtgataat gctgaggcgt aatttaaacg gacgttcttt cagaccttca
      181 aacggaatgc atttaaattc aaatgccact ggcataatgt ctttggtctt cgcttcgaca
      241 gactccatca gggagcgttt gccgctgaag tcattatctt caaaatcagc ggtctggttt
      301 gcttcaatcg tgattttacg gaccgccgca gccgcttttg ttgcctgaat ggtgtcacca
      361 ttagcatcaa agcccacaag gtagtcggcc cagtcttcaa tccattctgc cagtgatttc
      421 tgggagttac gctcgccatt aacagacaac agagcagaga acggtgctgt ctttttcagt
      481 ttgagagtgg cggtgttatc tgcgtgacct ggttcatcaa tagtacccag gttaagcaca
      541 ctgacggcac gcatattatc agcatcgata aagcagcggg tgccttcatc tgcaagatct
      601 ttagaataac gggtaaagtc atcgatgctg gcagtggaaa gcgcaccacg gaaacggaag
      661 cgatttaaat taaatttttc cagatcatga atgcggaaat tctcaggcaa tgccacagca
      721 tcggcaccaa tcttactgat aatttcatta acaccctgag cagaaataag ggcatggatt
      781 tgattaattg cggttgcgtc taagttctga gacataataa gtcctaacta tataaagata
      841 ttcagtgatg agataaataa tcagttaatt aagaacgata ttaatgacct gctgcgcgga
      901 gttttccgtc aggttcaccg gcaagagtca gtaattgtcc ctggtcttcc tgcagaatag
      961 tcaggcgacc accgcgattg acatacatcg gcgtttcggt ggtgtcttct tcggaaattt
     1021 tcccgcggtt agtcgggcga acatatgaga gtttgtgttt gattttcaca cggttctcat
     1081 caaatggttc gatttccagg ttgagtgaga ccttaccttt ggttttcgtg ttcatcacac
     1141 cggaagcgac ttcactgaga actgcgccga ttttggtttc aaatacgccg ccgtccagct
     1201 ccccgataaa tgcctgcaca tcagtactgc gttcgctagc cattttgctg ctcctcatca
     1261 tatcgaccct gcaaggccga ttagtttctc cacaaaacag agaagaacac ctgcggtagc
     1321 agccgcccgg atggattggg ttatgagccc gtcgtccggt gatgctcttc tctgttttgt
     1381 aaaaaggacg gtaccagccg gaagcaaggg tacaaactgg taccgccagg actacacaca
     1441 gcataaagtt gtggtgccgg gtgcctcccg gtgcctggcg aaggttgcac accagacggg
     1501 tgggtatcca cagaaggtcg actgtcagcc tcaaccttaa cccgcgtgcg ctgagccgca
     1561 ttcaccacaa ctctaaggat tctctttggt tgaaaatact tagctgttat gtgcctgtct
     1621 tttcaccact tcaggctcgg tggtatcctt ttaagcccgt atacataaaa ggaaaatcaa
     1681 atgacttttg atgaaaaaga acttgataat gcaattaata aaatcatcgt aacgtcgctc
     1741 ttttcctgtc tcagcgacac tcagcagaaa cagttctacg aatcggcttt caacatgatc
     1801 gagcgttgtt gtttctgcga tgccgacgag ttacctgaaa aaatcaggaa acagttggct
     1861 gatgctcttc gagtgcgact ttctgaccaa ttttctgaaa tgtgctctcc gaatttggac
     1921 aaatagaaaa aggccatttc cattcagggt ctgatggaaa tacttcagcc tgttccaaag
     1981 cacggcgtaa agagaacaca actccagcca taatctgatg tttcccattg gtccagctat
     2041 cgccgctctg atctacaggg gcggctatgt cgtatgacca aacgacttca cagttattgt
     2101 ttaaaatctg gactttcatt tcatacacct gctttaacat gagtgcctag tggcacaaca
     2161 tgactcaacg aatcatcctg gacttcatat gccccaggcg gctacttcgt gggcgtcctg
     2221 cctgttcgtt gtttcgcttg ggtacattat gtatcccaag ggtacattgt caagtataaa
     2281 aaaacctgcc gaagcaggtt cataaacatt gattaggctt tgattttgta tcttcttggt
     2341 tttcctgaga aaatcactgt accaattata gagcaattac cgttgatctt aatgtaaggc
     2401 tcaggccagt ttgggtttaa cgctttgaga taacgctgtg tcccatcttc tatcaacctt
     2461 ttgaaggtgg tttcgcctgt atcgtgcatc aatgcaataa cgtcgtcacc gtggcaggca
     2521 ggtacttcag gatcgacaaa aatcatgtct cccgggcggt actcatcaat cattgaatca
     2581 cctatcaccc gcaagatata agtcatttcc ccacagggta cagggcaggg atacgtttct
     2641 gctgtgctca aatcaacctc agaatatcca acttctttcc atgctccggc ctgtacccat
     2701 gatatgacag ggactaatgt gatttgttta ttagtgattg aaacatcagg tttttttgtg
     2761 atgttcgttg tctggtgttc ttgatcgagc catccgacag gcaggtcgaa acatttttcg
     2821 atgtgtcgtg ccatgctgtc accgatattt ttagtagcac catctcccat aaacctgctg
     2881 gtctgggttg gctcgcgatc aatcatagtg gcaaaggaag aattcccgcc aacaccatct
     2941 ctcagttttc tggcgttaga ccgccggatg tcatggattg ttttcataac gaaattaaaa
     3001 cccttgtacc gttaaggtac aagtgtcttg gaggttcatt tcaatcatgt aacatgtaca
     3061 ccggaggtac atattgtatg aaagcgtatt gggactcttt aaccaaagaa cagcagggcg
     3121 agttggccgg aaaagttggc tcaacacctg gctacttacg gctggttttc aatggctata
     3181 aaaaagccag ttttgtgctg gctaaaaaac ttgagcaatg cacgtcaggt gcaattacga
     3241 aatctgactt aagaccggat atctatccga aagattagca gaacactttc aatttttaac
     3301 cacagaacga tgaggctaac cgtgggtaag catcactgga aaatagaaaa acagcctgag
     3361 tggtacgtga aagctgtcag aaaaactatc gcggcgttgc cgggtggtta cgctgaagcg
     3421 gctgactggc tcgatgtaac agaaaacgct ttattcaacc gccttcgtgc agatggcgat
     3481 cagattttcc cgctgggatg ggcaatggtt ttacagcgtg ctggtggcac tcacttcatt
     3541 gctgatgctg tggcgcagtc tgcaaatggc gtctttgtgt ctcttcctga cgtcgaggat
     3601 gtggacaacg ccgatatcaa ccaacgcctg ctggaagtca ttgaacagat cggcagttat
     3661 tcaaaacaga ttcgttcagc aattgaagac ggtgtagtgg aaccgcatga gaagacagca
     3721 attaacgatg agcagtacct ctcaatttcg aagctgcagg agcatgcagc actggtctac
     3781 aaaatctttt gcgtttcaga aagtagtgac gcccgcgagt gtgcagctcc gggcgccgtg
     3841 gcatgtcgtg actgtggaga aactaacgca tgaacagttt aacaacacac taccgtcgct
     3901 cgcaactgat tgcgcttcct gtaccgggtg gaaaagcgaa ggtggagtat tgctatgcag
     3961 taaatgtacc aggtgacagg gaaattgtaa cccacagctt tgcagagtgg gctgtgggtg
     4021 atttcaaccg gcagaaggag acagtccttt gcgacaagtt aaccgctggt tcaaagagca
     4081 ctacggagtg cccgtcagag tcattcgttg ggagccggaa acacaacgtg ttatctacct
     4141 ccgcgaaggc tatgagcatg agtgcttcag cccgctcgaa cagtttcgtc gtaaattcag
     4201 ggaaatagag gtcggtcatg agcactaaat taaccggcta tgtatgggat ggttgcgctg
     4261 catcaggcat gaaattatcc agcgtggcaa ttatggcccg cctggctgat ttcagtaatg
     4321 acgaaggtgt gtgctggcca tcaattgaaa ccattgcccg ccagattggc gcgggaatga
     4381 gtaccgtcag aacggctatc gcacggctgg aagcagaagg ctggttaacg cgtaaggcgc
     4441 gtcgccaggg taaccgcaat gcgtcgaatg tttatcagct taacgttgcg aagcttcagg
     4501 cagcggcatt ttctcaactg tcagattctg acccgtcaaa atctgacgca tcaaaatctg
     4561 acccgtcaaa atttgatgcg tcgaaatctg gcaaaaaagc gggttttcac ccgtcagaat
     4621 ctggcgggga tccgtcagta aaatcaaaac atgatccgtc agataaaaaa acttctcgtc
     4681 cggacgcttc gcaaccggac acgcagacgg ctgaacagga gtttttaact cgccatcctg
     4741 atgcggttgt attcagccct aaaaagcgcc agtggggaac gcaggatgat ttgacctgcg
     4801 cacagtggct ctggaaaaaa atcatcgccc tgtacgagca ggccgccgaa tgtgacggcg
     4861 aggtggttcg tcccaaagaa ccgaactgga cagcctgggc aaacgaaatt cgcctgatgt
     4921 gtgtgcagga tggtcgtact cataaacaaa tctgcgagat gtacagccgc gtcagtcgcg
     4981 atccgttctg gtgccgtaac gtgctcagcc cgtcgaagct gcgggaaaaa tgggatgagc
     5041 tttccctgcg cttatcgccg tccgtcagca cgtacaccga aaaacgcgaa gacccgtact
     5101 tcaaagctag ttacgacaac gtggactaca gccaaatccc ggcaggattc agggggtgag
     5161 catgagtctt ttgaatgacg ttcagaaatt cattgaagcc catcggggct gtacttccgg
     5221 agatattgcg gatgcttttg ccggttactc acggcagcgc gttctgcagt cagcaagcaa
     5281 gttacgtcag agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc
     5341 gcgcctgact gagagagcgc aggaaccgga accacaacca gttcgtgaaa ccagacctgt
     5401 gcgcaatttc tatgtcggca ctaacgatcc ccgtgtgatt ttgtgcctga cccgccaggc
     5461 ggaagaactg gagtccaggg gcttataccg tcgtgctgca accgtgtgga tggcggcatt
     5521 ccgtgaaagc cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcggtgctt
     5581 acggaaaagc agcaagcgcg ctgcatcggg tgaagagtgg tatctgtcag ggaattacgt
     5641 gggggcttaa tgagtaataa atattgccag gcgctggtgg aactgcggaa caaaccagcc
     5701 catgaactga aggaagtggg cgatcagtgg cgcacgccgg acaacatttt ctggggaatt
     5761 aacaccctgt ttggcccgtt tgttctggat ctgttcactg acggtgataa cgccaaatgt
     5821 gccgcgtatt acacggcgga agacaacgcg ctggcgcatg actggtcaga acgtcttgcg
     5881 gagcttaaag gtgctgcctt tggtaatccc ccatacagcc gcgccagtca gcatgagggg
     5941 caatacatca ccggcatgcg ttacatcatg aaacatgcca gtgccatgcg tgataagggc
     6001 gggcgctatg ttttcctgat caaagctgcc accagcgaag tgtggtggcc ggaagatgca
     6061 gatcatattg cttttattcg cgggcgtatt ggttttgaac tgcctgtctg gtttatcccg
     6121 aaagacgaga agcaggtact gacaggagct tttttcgctg gtgctattgc tgtttttgac
     6181 aagacctgga agggaccggc aatcagctac atcgggcgcg atgaacttga ggcatgtggt
     6241 gaggcgtttc tggcgcaggt tcgccagcag gcggaaaaac tggtcaggga gatggcggca
     6301 tgacgacgtt aactcaatgc cagcagcagg tgctggatat gctgatttct tatcagaaag
     6361 aacgtggctt cccgccaacc aatcaggagg tggcaaccat gctgggatac cgttcggtga
     6421 atgcagcggt ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg
     6481 gcgtggcccg ggggataacg cttcataccg cggtgaagga cgacgacagc gaggcggtcg
     6541 ggattatccg cgcactgctt gccggtgagg aaaacgcaag gctgcgtgca acccactggt
     6601 tacatgagag ggacctgaaa gtatgaagct gatcctgcct tttccgccca gcgtgaacac
     6661 gtactggcga caccccaaca aaggggcgtt tgctggtaag agcctgataa gcgcggcggg
     6721 gcgaaaattc cagagcgcgg cgtgcgcagc aatagttgag cagttacgtc gtctgccgaa
     6781 accaacgtcg gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg
     6841 cgatctggac aactataaca aggcgctgtt tgacgccctg acccacgcgg gtgtgtggga
     6901 agacgacagt caggtgaaaa gaatgctggt ggagtggggg ccggttatcc cggaagggaa
     6961 ggtcgagatc actatcaata agtacgagaa aacggcgggt gcagccgcct gattaagagg
     7021 agaaacgaag tatgaataat ctgatggtca ttgatggtat tgaagttcgt cgtgatgctt
     7081 atgggcgtta cagcctgaac gatctgcaca gggctgccgg ttctcaggat aagcataagc
     7141 ctgcattctg gctccgcaat gagcaaaccg aacgtttaat aagcgagttg cagatttgca
     7201 actcggtcaa tatagagcca gttaacgtta gtcgtggcgg aaataaccag gggacgtatg
     7261 tctgcaaaga actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg
     7321 tgatccgtac tttcgatatg gtaaccagcg caccggaaaa attatccggg caggctgctg
     7381 acaagatgca ggctggcgtg attctgctgg actttatgcg gcgggagtta aacctgtcta
     7441 actcatctgt gcttggggcc tgtcagaaac tccaggaggc tgttggctta ccgaatctgg
     7501 caccacgcta tgccattgat gctcctgctg acgcgcctga tggctcaagt cgccctacgc
     7561 tgtcgctgag tgcactgctg aaacagtatg gtatccgcct gacagctaat caggcatatc
     7621 accagatggc gaagctgggg atcgttgaac aacgcgaacg atacagccgt accgcgatta
     7681 acaacatcaa aaaattctgg tcgctgacgg cgaaaggctg catgttcggc aagaacatta
     7741 ccagtcctgc aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc
     7801 tgttaaagct gctcgatacc gttcattgag gtgaccgtga gagcactact gacccctgaa
     7861 attgccccgc gtatggggat cgtattgttc agaccaggtt cagagctgat gcccctgttt
     7921 atgcaggggc gtgtactgct ggagcctgag ccggaacgtt attcatcttt cgccagtggt
     7981 gccgttcccg cggcatcaca accgctggcg gatgatcctg ctgttcgggc cgtgttccgc
     8041 aatgaggcag tgatccgtcg tgctggtggc gtggaatgtc ttgaaagctg gctacttcgt
     8101 gaaaaaggct gccagtggcc tcattccgac tggcacagcg agaacatgac cacaatgcga
     8161 cacgctccgg gcgcaatccg tctgtgctgg cactgtgata accagctgcg cgatcagttc
     8221 acggaacggc tggaatcaat ggcaacggat aactgtgccc gctgggtgtt gtctgttgtg
     8281 cgtcgggatc tcggttttga tgatagtcac gttgtgacaa tgccggaact gtgctggtgg
     8341 ctgattcgta atgacctggc ggatgcctta ccggaaagtg cagcccgtaa ggcactgaga
     8401 ttaccaaagc ctgttgtgcc gtctgtcacc cgggaaagtg accttgtgcc ttcggttcct
     8461 gccaccagca tcatccaaga taaggcaaaa aaggtgctgg cgctgaaagt ggatccggag
     8521 tcgccggagt cttttatgtt acgcccaaaa cgtcgccgct gggttaatga gaagtacacg
     8581 cgctgggtta agacacagcc gtgtgcatgt tgtggaaagc ccgctgatga tccccaccac
     8641 ctgataggcc acggtcaggg gggaatgggt acaaaagcgc atgacctctt tgtgttgcct
     8701 ttgtgcagaa agcatcacga cgagctgcat gcggataccg tggcatttga agagaagtat
     8761 ggctcccagc tggagctgat atttcgtttt atcgatcgtg cgctggcaat tggcgtgctg
     8821 gcctgatttg gtggagaaag ttgatgcgtg atattcagat ggttcttgag cgttggggag
     8881 catgggcggc gagtgatagt tcaggcgttg actattcacc tatagctgct gggtttaagg
     8941 gacttcttcc ctataccagc aagaaacgct tggcttgttc ggatagtgat gcattaatta
     9001 ttgaaggttg tcttgctcgt ctaaagcaaa aaagacctta tgagcattcg cttcttgtgg
     9061 cacattattt atacagaata tccaagcgta agattgcaaa ggcgcgtgga aaggatgaga
     9121 aattgatacg tatagaaata cagctagccg aaggatttat tgatggctgc ctttccatgc
     9181 tagatctaac attagatttg gacgtttaat aatacgcccc atgcaggggc gtattattta
     9241 ctggatgaat gacatttgat taatatattt tatcaataac tctctgggtg tagtactcca
     9301 aaaatttagg tgttcataat cagtataaac agtggtgaat tttttaagtt cttcatcact
     9361 tttaggggca aatcgctcat taagagtaat actgtcttta attattccga agaatacaat
     9421 agatatttca ggaatttgcg atcttctctc ataagaataa ggtatttctt ttatgttaag
     9481 cagatctgct aaatgagata tagattctgc ggtaatttct tgttttagtt tttccttgtc
     9541 accaaagcaa tgtatgaaga cagctacgac aaagatctta tttattaaag gatcactgct
     9601 tttgtactca tcgtcgttta atagttgaaa aatgttaaga ttgcgtgaaa aagtttgtgt
     9661 ttcacgtaag gataagttgg ttcgttgaat taaatcacgg ataaatctac cagataacct
     9721 attaatttca tttaatgatt ttgtcttgtc tacaaggtaa ttccaatata taacagatgt
     9781 tttacatacg acgtgaccat ttattaaaca tgtgtctgga agcgtgatgg tatattttat
     9841 aaacttgtca agatactttt gtgagttaat actataacca taaatatgat ttatcgatgc
     9901 ttttaattgt tctgtgtttg taactaaaat aaaaaagaca ttattgatgt caaaaatgtg
     9961 ttttattgtt tcaatgacat ttgttgaaaa actcggctta catcggtcta attcatcaat
    10021 tataattact atcttttgac tttctgaaat gctttcgatg caagatttaa gtgaatttat
    10081 gtttttctct gattccatat ggtcttcaag caaattttca atagtcccat ctattgctgc
    10141 attgcttgct ttcttcatcg catcttggaa ttcttcggaa acttcactag cctcctgtcg
    10201 taaaaaccaa cctgcaccag cttttagtac cgtttttaaa ccaaagcgaa ttgcaggaag
    10261 agatctctta atgaagtgtt gtttttcttc ctcaggtaaa atgctggcaa ttgcagaggt
    10321 tatgagaagt aatggagatt ctgcatgatc ccctttaaag gcatcaatat aaacaacttt
    10381 agattcagtt tcttgctcaa taatgagatt tttcagtttg atactaaatt ctgttttccc
    10441 tgttccccat gcgccgtcta ttaccagtgg tgaaatgtct gcctctggtt ttagcaactt
    10501 gatgatattt tcagcgatgt ttctgcgttg gaactcgtca cgttcagtga aagatagtgt
    10561 atctaacata atataaacct attaactcct acagtaaaaa acgaataata cgactttggg
    10621 attaaaaatc attaacgcgg tccgcaaaaa ttcttgtaat ctgttaagag tggttacttc
    10681 gccacacagc ttaaacccgc cgtcgagcgg gttttgtcgt ttttgttgct ggggattcgt
    10741 tgggcctggc ctatcccgca gttatccatt ggttcggctc tttgacgttt ccgcttctga
    10801 tttgcggtac atgatgttcc ctcaatttgc acctgctgta tcagcgaggt gagagataac
    10861 tacaaatgcc tcataaccca aatacctggt tggagttggt ccagagctgg tggcgtggag
    10921 acacaccgct gggcgcagtg attatgtcga ttgttatggc tggcttgcgc attgcatatt
    10981 ttggcggtgg tgggggctgg aaacgaaaaa cgctcgagat tttgctctgt ggtgctctga
    11041 cgctgacttt tgcatccgct cttgagtatg tcggatggcc taaatctctt tctgttgcca
    11101 ttggtggcgg cgttgggcta atcggggtcg atgctattcg tggggctgca atgagagtaa
    11161 tcggtaataa gtttggtggc tctaaggagt aatttatgca ggtactaaat tcccagcgta
    11221 aagctttcct cgatatggtg gcatggtcag aaggaacgga taacgggcga caaccgacac
    11281 gtaatcacgg ttatgatgtt attgttggcg gcgaactgtt tactgattac tctgatcacc
    11341 ctcgcaaact tgtcacgcta aaccccaaac tcaaatcaac agccgccgga cgttatcagc
    11401 ttctttcacg ctggtgggat gcctaccgta agcagcttgc tctgaaagac ttctccccca
    11461 aaagccagga tgctgtggca ctgcaacaga ttaaagagcg tggcgcttta ccgatgattg
    11521 atcgcggtga tattcgtcag gctatcgacc gttgcagcaa tatctgggcg tcgttacccg
    11581 gtgcaggtta tggtcagtat gaacataaaa tcggtgattt gattgcccgg tttaaagagg
    11641 ctggtggggt ggtaaatgaa gctgagctat aagctgctta tcgttggttt cttcgttact
    11701 gtcattggtt ctttcatctg gtcggccaac cactactaca gcaaatatca gtacgaaaag
    11761 aaacgtgctg atgaggctgt acgaaatgct gaatcagcaa ctgccattac caataacgtc
    11821 ctgcaatcac tacaaatcgt caatacagtt ctggaggcta accagcatgc aaaacagcag
    11881 atcgcactgg agtcacagag agcccgggaa gatatcaaag tggctgttgc ggatgatgat
    11941 tgtgcttcac gccctgtgcc tgatgtcgct gctgaccggt tgcggaagta cgcggacagt
    12001 atacgtgcag gttccaacga tgccgttacc gacgaacctg ctcgctgaaa ctccacagcc
    12061 agttataccc aatcctctga cttatgggga tagtcttagt ttgaatgtaa gtctgctatc
    12121 agcactgggg ctatgtaacc gtgataagtc tgatcttcgt aggttaggag agcaaaagta
    12181 caatctgcat ttgaataata atattcatta ggtgaaatat tattatttga ctgttctagt
    12241 tattatgcat ttagttacaa tactctcact attaacagtg aggtaaaaat gaacgaaaat
    12301 tatattgcat atgagacact tgtagcaaac cgtgctgctg ctgagtgggc ttgttgggca
    12361 atgattgcat cttgggtgag tgctggagct actattgtta ctttggtttt ggcgttcaag
    12421 gcattgttta cgtggcggga acaagagaaa acaaaagtaa aaatagattt taggaatgca
    12481 ttaaagaaat taaagacagc tctattattt atgcctgtca atattgaccc cgagaaactc
    12541 aatgatgaac gagagcaagt tattgcgaaa tggctattta aagatgtaga tcttattagt
    12601 cagcaaattg agttgggaga agagaatgtt aaaagatttg atgaactttt gagtattttc
    12661 gattgttgcc agtcttcatg gtttgcgaca gagcacttat ttgataatac tgagttagaa
    12721 aaagtttggc atgagttcga gtctaacttt aataaatata taaatggtgg tgagagtaag
    12781 gatttactta tgaaaatgct tgataagctc atctcttcta gatttgtatt tgagtcaaga
    12841 taattgcctt tgagtatttt tctttattat tttacttatt ataaattttt tatatgcccc
    12901 ctagaatccc aaaagcctgc cgtgttcgcg gctgccgctc tacaaccacg gacccgtcag
    12961 gctactgcga aagccataaa agcgaaggct ggaagcaata caagccagga caatctcgtc
    13021 atcagcgcgg ttatggttcg aagtgggatg ttatccgcgt gcgtgtcctg aagcgtgaca
    13081 aaggcctgtg tcagctatgt ctgcgtgctg gtgtggtgcg tgaggcgaaa actgttgacc
    13141 acatcatccc taaagcgcat ggcggcacag atgccgacag caatctgcag agcctgtgct
    13201 ggccgtgcca taaggagaag acggcccgtg aacggctaaa gtgatcataa ttctcaactg
    13261 tctgagggga ggggcgggtc aaatctctgt gacctgacgt cttccggact gcccgcccca
    13321 tcgttttttt atacccgcga aaaatgaaat ttaaccagga gtgccgcata tggctggaac
    13381 ggcggggcgt tccgggcgtc gccctaagcc aacggcgcgc aaggcgctgg ccggaaaccc
    13441 cggcaagcga gccctgaaca aagatgaacc tgtttttacg cccatcaaag gtgttgagcc
    13501 accggagtgg ttcgctgaag aagagcttcc tctcgccacg atcatgtggc aactgacaac
    13561 caaagaactc tgcggtcagg gcctgctgtg cgtgactgac cttgcggtgc ttgagcggtg
    13621 gtgtgtggcc tatgagttct ggcggcgtgc cgtgaaaaat attgccagac agggcaacac
    13681 catcaccggt gcaatgggcg gtatggtcaa aaatccggag ctgaccgcca aaaaagaaca
    13741 ggagtccgag atgagcagca cgggggcaat gctcggactc gaccccagta gccgccagcg
    13801 tctgattggc ctggcggggc agaagaaagc cactaacccg tttctgaaaa tcatcgagtc
    13861 atgagccgga aatcttaccc caacgtaaat gctgccaatc agtatgcccg tgatgtcgtg
    13921 cgcggaaaga ttgtggcctg ccagtttgtg attcaggcct gccagcgcca tcttgatgac
    13981 ctgatggcgg aaaaaagtaa gtcgtttcgt taccgcttcg acaaggagct ggctgaacgg
    14041 gccgcgaaat ttattcagct gttgccacac accaaggggg agtgggcatt caagaggatg
    14101 cccatcacgc tggagccgtg gcagctcttt gtgatctgct gtgcgtttgg ctgggtcaat
    14161 aaagggtccc ggctgcgccg cttccgggag gtgtataccg aaatcccccg taagaacggc
    14221 aaatcggcaa tctctgccgg tgttgccctg tattgttttg cctgtgataa cgagtttggc
    14281 gcggaagtgt attccggtgc cacgacagag aaacagacgt gggaagtctt tcgcccggcg
    14341 cgactgatgt gtaaacgcac acccatgctg acggaagcgt tcgggattga ggttaacgcc
    14401 tcaaacatga accgtccgga ggatggggcg cggtttgaac cgctgattgg taaccccggt
    14461 gatggttcat caccccactg tgcggtggtg gatgaatatc acgagcacgc cacagatgcg
    14521 ctttacacca cgatgcttac cgggatgggg gcgcgacgtc agccactgat gtgggctatc
    14581 actaccgccg ggtacaacat tgaggggccg tgctacgaca aacggcggga agtcatcgag
    14641 atgctcaacg gttcggtacc caacgatgaa ctgttcggga ttatctatac cgttgatgaa
    14701 ggtgacgact ggaccgaccc gcaggtgctg gaaaaagcca atccaaatat tggcgtgtcg
    14761 gtttatcgcg aatttttgtt aagtcagcag cagcgtgcga aaaataacgc ccgtctggca
    14821 aacgtcttta aaacaaaaca cctcaatatc tgggtgtcgg cgcgttcggc gtatttcaac
    14881 ctggtgagct ggcagagctg cgaggataaa tcactgaccc ttgagcagtt cgaggggcag
    14941 ccgtgcattc tggcctttga cctggcgcgt aagctggata tgaacagcat ggcgcgactt
    15001 tatacccgcg agattgacgg taaaacgcat tactacagtg tggccccgcg tttctgggta
    15061 ccgtatgaca cggtgtacag cgtcgagaaa aatgaagatc gacggacagc cgaacgcttt
    15121 cagaaatggg tggaaatggg cgttctgacc gttaccgatg gtgcggaggt ggattatcgc
    15181 tacatcctcg aggaggccaa agcggcgaac aaaatcagtc cggtcagtga gtcacccatc
    15241 gaccccttcg gggcgaccgg gttgtcacat gaccttgctg atgaagacct gaaccccatc
    15301 actatcattc agaactacac caacatgtcc gacccgatga aagagctgga agcggcaatt
    15361 gaatcggggc gctttcatca tgatggcaat cccatcatga cctagtgtat cggcaacgtg
    15421 gtcggcaaaa ccattccggg taacgatgat gtggtgaagc ccgtcaaaga gcaggcggaa
    15481 aacaaaattg acggtgcagt tgcgctgatt atggcggttg gcagagccat gctgtacgag
    15541 aaagaagaca cgctgtctga ccacattgag tcctatggga tccgctcgct ttaactgagg
    15601 taattatgat catgctgatt ctcgcgcctc tggtgggcgt gctgggggcg cttttgctgg
    15661 cgtatggtgc ctggctgatt tatcccccgg cggggtttgt tgttgccggg gcgttgtgcc
    15721 tgttctggtc gtggctggtg gcgcgatatc tcgaccgtac acagtcgtct gtcggcggag
    15781 gtaaatagtg ttcttttcgg gattatttca acgaaaaagt gacgcaccgg tgaccacgcc
    15841 agcagagctg gcggatgata tcgggttgtc ctacgacacc tataccggaa agcagatcag
    15901 cagccagcgg gccatgcgac tgacggcggt tttttcctgt gtcagggtgc tggcggagtc
    15961 ggtcgggatg ttgccctgca acctgtatca cctgaacggc agcctgaagc agagagccac
    16021 tggcgaacgt ctgcataagc tgatctccac gcatcccaat ggctatatga cgccgcagga
    16081 gttctgggag ctggtggtca cctgtctgtg cctgcgggga aacttttacg cctacaaagt
    16141 gaaagcattt ggcgaagtgg ctgaactgct gcccgtcgat cccagctgtg tggtaccgaa
    16201 gcttaacagt agctgggagc cggtctatca ggtcacattc ccggatggct ccgcggatgt
    16261 actgagccag gaggatatct ggcatgtgcg cacgctgacg ctggacggac tggtggggct
    16321 gaatcccatc gcctatgccc gcgaggcaat atcgctggcg gcagcgaccg aagagcacgg
    16381 ggccagactg ttcagcaatg gcgcggtgac gtcgggtgtg ttgcgtacag agcagacgct
    16441 gtcagatcag gcttacgagc gcctgaagaa agattttgag gagcgtcaca ccgggcttgg
    16501 caatgctcac cgcccgatga tccttgagat ggggctggac tggaagtcga tggcgctgaa
    16561 cgccgaggac agccagttcc tggaaacccg caagtttcag cttgaagaaa tctgtcgtct
    16621 gttccgggtg ccgttgcaca tggtgcagaa caccgatcgc gccactttca acaatattga
    16681 agagctgggg ctgggattta tcaactattc actggtgccg tatctgaccc gcatcgaaca
    16741 gcggatcaac accggactgg tacgaaaaag taagcagggc gtttattacg ccaaatttaa
    16801 cgccggggcg ttactgcgcg gggatatgaa gtcccgtttt gaagcctacg ccaccgggat
    16861 taactgggga atttactctc ccaatgactg ccgcgacctg gaagatatga atccgcgtcc
    16921 cggtggtgat gtctatctca caccgatgaa catgaccacg aaaccctccg atggcagtaa
    16981 agccggtaag cagaaggata acgccaatgc agacgaaaca acgtcttgat gtaccgctga
    17041 gtctgaaatc tgtcagtgac tccggtgagt ttgaagggta tggctccgtc tttggtgtaa
    17101 aggacagcca cgatgatgtg gtgatgtccg gggcatttgc cgcttccctg cgggcgtgga
    17161 gtgacagaaa agcgttacct gcgctgctct ggcagcaccg catggatgag cccatcggtg
    17221 tttacaccga aatgaaggaa gacgatgtcg ggctttacgt cagggggcgg ttgctcattg
    17281 atgatgatcc cctggcaaaa cgcgcacatg cacacatgaa ggccggttcg ttaaccggcc
    17341 tttctattgg gtacgtcctg aaggactggg aatacgaccg gacgaaagaa gcctttctgc
    17401 tgaaagaaat cgacctctgg gaagtcagtc tggtgacgtt tccgtctaac gacgaggcgc
    17461 ggatcagcga cgtcaagaac gcgctggccc gcggggaaat ccccgaacag aaaaaaatcg
    17521 aaagagtcct gcgtgatgtc ggactctccc gtacccaagc caaagcattc atggccgggg
    17581 gctatatcgc actgtccctg cgcgacgctg aggatgtggg ctctgcactg aatgcactga
    17641 aaaatctgaa cttctaatca ggagaaatac gatggcggta gatattaaag atgtcgaaca
    17701 ggtcgcgcag gagctgcagc agaagtttga cgacttcaaa gcaaagaacg acaagcgcgt
    17761 ggatgcgatt gagcaggaaa aaggcaagct tgccgggcag gtggaaaccc tgaacgggaa
    17821 actcagcgag ctggaaaatc tcaaaagcga tcttgaaaaa gagctgcttg agctgaaacg
    17881 tccggcaggt ggcgcgcaaa ataaactggc caccgagcat aaagaagcgt ttgtgggctt
    17941 tttgcgtaaa ggccgtgaag atggtctgcg cgatctggag cgtaaggcat tgcaggtggg
    18001 caccgatgaa gacggtggct atgccgtgcc ggaagcgctg gatcgcaaca ttctcaccct
    18061 gctgaaagat gaagtggtga tgcgccagga agccacggtg atcaccgttg gcggttccga
    18121 ctacaaaaaa ctggtgaatc tgggcggcac ggcttccgga tgggtggggg aaacggatac
    18181 gcgatcccag actgccacct ccagactgga gctgattgaa cctctcatgg gggaaatcta
    18241 cggcaacccg caggccaccc agaaaatgct ggacgatgcc ttcttcaacg tggaggcctg
    18301 gatcaacagc gagctggcaa ccgaatttgc cgaacaggaa gaaattgcct ttaccaccgg
    18361 cgatggtacc aagaagccga aagggttcct ggcgtatgaa tccactgatg aaaccgataa
    18421 ggtccgggcg ttcggcaaac ttcagcatat tgtatccggc gaagcgacgg cggtgaccgc
    18481 agacgccatt atcaaactga tttacacgct gcgtaaggca caccgcaccg gcgcgaagtt
    18541 catgatgaac aacaacagcc tgtttgccat ccgtctgctt aaagacagcg agggtaacta
    18601 tctgtggcgt ccggggctgg aactggggca gccgtcctct ctggcgggtt acggtatcgc
    18661 tgaaaacgaa cagatgccgg atatcgccgc tgatgcgaaa gccattgcat ttggtaactt
    18721 caaacggggt tacaccatcg ttgaccgtat cggcacccgc attctgcgtg acccgtacac
    18781 caataaaccg tttgtcggtt tttataccac caagcgcacc ggcgggatgc tggtcgattc
    18841 gcaggccatc aaactgctga agattgcagc ggcgtaatca ctcaggggcg cggaaccgcg
    18901 ccccctgttc tgacgggtga agaatcatga tcctgaaaca agatctgaaa tggtcaccgg
    18961 acggtatgcg tgttgaggtc attcgggccg gtgagtatga cgacggggcg cttcctgccc
    19021 gggtgcagga gattgcactt caggccgggt tagcagagcg cggaaccagt gcaaaaagca
    19081 gtaaagcgac aaaagagaaa aaagccacga ccagtaaaga gggctgagta tgcttctgac
    19141 aatggaagag attaaagccc aactccggct ggatgaggat ttcgatgctg atgaccgcca
    19201 tctgcaactg ctggcctgtg cggcacaaaa gcggacggaa acgtatctga accggaagct
    19261 ctatgcaccg gatgaaacca ttccggacag cgatccggac gggctgcacc tgccggatga
    19321 tattcgtctg gggatgctga tgcttatcag ccatttttac gaaaaccgct cgtcggttac
    19381 ggaagtggag aaacttgaca tgccgcagag ttttggctgg cttgtcggcc cgtacaggta
    19441 ctttccgcaa tgaaaattcg tcaggcgcag accagcgcaa cctacattct gccggacccc
    19501 ggtgaactga ataaacgcgt cctgatccgc ctgcgggtgg atatgcccgc ggataacttt
    19561 ggcgtggagc ctcaatacct gattacgttc cggacatggg cgaaggttat ccagaccagt
    19621 gccaccacct ggcaggaaac cgcgcagacc ggggacgcca tcacccatta catcaccatt
    19681 cgttaccgcc gggggattac cgctgattat gaggtggtct gcggtgacag tgtgtaccgg
    19741 gtgaaacgtc agcgcgatct gaacggggcg cggcgctttc tgctgctgga gtgtacggaa
    19801 ctgggcgaat gtgggcagag tcacggaggc aacaatgacg acttcctttt tgcacgttga
    19861 ttttcagcag cccgcggaga tgcgctttaa ccgcgcccgt gtccggcggg cgtttgtcac
    19921 gattggtcag cgtcatatgc gtgatgcccg tcggctggtg atgcgccgtg cgcggtcggc
    19981 accgggtgaa aaccccggtt atcagaccgg acgcctggct cgttcgattg gttacatggt
    20041 acccagagcc agtaaacatc gccctggttt tatggcacgt atagccccta accagcgtaa
    20101 tggagaggga aaccgccgta tcaccggtga tttttatccg gcttttttgt tctatggcgt
    20161 gaggcgaggg gcaaagcgtc gtcgcagcca tcatcgtggt gcatccggtg gcagcggctg
    20221 gcgactggct ccacgtaata actttatggt ggaaacgctt gaaaagaacc gcagctggac
    20281 acgctatttt ctggcgcggg aattgcgtaa atcactgaag ccggagcgac gacacagatg
    20341 aaactgacgc ctgttattgc tgcgctgcgt gcccgctgcc cgtattttga aaaccgggtg
    20401 gcaggcgcgg cacagttcaa aaatctgccg gaggtcggaa agctgagact cccggcggcg
    20461 tatgtggtac cgggtgatga ctctccggga gaaaacaaaa gccagaccga ctactggcag
    20521 gagctgaaag agggcttctc cgtggttgtc atactgagta acgggcgtga tgagcgcggt
    20581 cagtttgcct cgtatgatgt ggtggacgat gtccggcaaa tgctctttaa ggccctgctg
    20641 ggctggaacc cggaagcgtg cggtaacccg attacctatg acggcggcac gctgctggat
    20701 ctgaatcgtc atgagctgat ttatcagttc gatttttcgg tcatcagcga gttgaccgaa
    20761 gacgataccc gccagcagga tgacctgaac agtctggatg aactgcgaac gctggcgatt
    20821 gatgttgatt atctagagcc cggtaacggg cctgacggcg atatcgaaca tcacaccgaa
    20881 ataacccttc cttcctgaga atcttcatgt ttgtgaaacc tgttaaaggg cggtcagttc
    20941 ctgaccctgc ccgcggagac cttttgcccg ccgaggggcg aaatgttgat gagaacaact
    21001 actggctgcg ccgtgaagca gcgggtgata tccggcgcgt gaatgaaaag gtgaataccg
    21061 atgacgagct ttaacaccat tccgtcaaat acgctggttc cgatttttta tgcggaaatg
    21121 gataactcgg cggcgaatac tgcacaggac agcggagcat cgctgctgat tggtcatgcc
    21181 aataacggtg cagagattgt tgccaacagt ctggtgctga tgccatcggc agactatgca
    21241 cgccagattt gtggtgcggg aagtcagctg gcgcgtatgg tcgaggctta tcgccagacc
    21301 gacccgtttg gtgagctgta tgtgattgcc gttccagaag ccacaggcgc ggcggcaacg
    21361 gttacgctga cggtgaccgg ggaagcaacc gaaagcggca cggtgaatgt ctatgtggga
    21421 cgtacccgcg tgcaggctcc ggtgaccaac ggcgataacg tcacgacgat tgccagcagt
    21481 atccaggatg ccatcaatgc cgttccggcc ctgccgttta cggcttcatc ttcggcaggc
    21541 gtggtcacac tgaccgcgcg tcataagggg ctttgcggga atgaaattcc tgtcagcctc
    21601 aattactacg gctttggtgg gggcgaagtg ctgccagcgg gcgtacagat tgccgtggcg
    21661 acgggtaccg ccggaacggg tgctccggtt ctcaccggcg cggtggctgc aatggcggat
    21721 gagccgtttg attatatcgg cctgccgttc aacgacacgg cctccgttaa cacgctggtg
    21781 accgagatga acgataccag cggtcgctgg agctatgcgc gtcagctgta tggtcatgtg
    21841 tatacggcaa agatcggcac gctgtcagaa ctggtgaccg caggtgacca gtttaaccag
    21901 cagcacatta ccctggcggg atacgaaaaa gagacccaga cgcctgccga cgagctggcg
    21961 gcaagccgta ccgcccgcgc agcggtgttt attcgcaacg atccggcacg tcccacgcag
    22021 accggtgagc tggtgggtat gctgcctgcg ccgaagggga aacggttcac gatgaccgaa
    22081 caacagaccc tgctgtctca tggcgtggca acggcgtatg tcgaaagcgg ggtgctgcgc
    22141 attcagcgtg atgtcaccac gtacaggaaa aacgcttacg gtgttgcgga taacagctac
    22201 ctcgacagcg agacgctgca taccagcgcg tatgtgctgc gcaaactgaa atccgtcatt
    22261 accagtaagt acgggcgtca caagcttgcc agcgacggta cccgctttgg tcccggtcag
    22321 gcgattgtca caccggcggt gatcaaaggg gaactgctgg caacctaccg tcagcttgag
    22381 cgtgcgggga tcgtggaaaa ctacgaactg tttaagcagt acctggttgt ggagcgtgat
    22441 gccagcgatc cgaaccgcct gaacacgctg ttcccgcctg actatgttaa ccagttgcgt
    22501 gtctttgccg tggttaacca gttccgtctt cagtattcag aggagtctgc ataatggccc
    22561 gtatcggggg aacctgttat ttcaaaattg acggtcaaca gctatcgctg accggcggca
    22621 ttgaggtgcc catgaacagg acggtcaatg atgacatcat cggcctggac ggttcagtgg
    22681 accgcaagga aactcaccgt gcgccttatg tcaaagggac cttcaaggtg ccgaagaatt
    22741 ttccggcgag caaaatcacc tcgtctgatg agatgaccat cactgccgag ctggcgaacg
    22801 gtcaggtcta tgtactgtcg tctgcctggc tgcacggcga agcgaaccat aatgccgaag
    22861 aaggcacggt cgatcttgag ttccacggtg aagaagggga ttaccagtaa tgaaagagct
    22921 tgagttaaag aaaccgatta ccgctcatgg cgagacactc tccgtactgg agtttgatga
    22981 gcccaccggg aaagatgtcc gcgagctggg gtatccctac cagatgaatc aggatgagtc
    23041 cgtcagactt ctggcgcatg tggtatcgaa atatattgtg cggctggcga aagtgccgca
    23101 aagctctgtc gaccagatgt ctccggcaga cctgaatgca gcggcgtggc ttgtggctgg
    23161 ttttttcctc caggcctgac ggctgaatac cttactgatc gcttctttga ttgcgccagc
    23221 tactggcgca ttaatccctt cgaattgctg aatatgccga tcagtgaaat tcccttgctg
    23281 gtcagtcagg caaacaggat agagcaggag aaacgcacac atggctgaat ttgagcttaa
    23341 ggcgttgatc accggtgtcg acaggctttc tcccgcgctg tcgaaaatgc aaaagaaaat
    23401 ccggggattt aaacgccagg cggaagaagc gtcacagggt gggctggcgc ttggtggcgg
    23461 actggcagcg ggtctgacgc tttccctgaa atcttatgcc gatcaggaaa acgccgccac
    23521 cgggctgaaa gtcgccatga tggatgcgaa tggcgaggtt ggaaagagct ttcaggacat
    23581 caataaactg gctattggcc tgggtaacca gctacccggt acaacggctg atttccagaa
    23641 catgatgcag atgctggtgc gtcaggggat cccggcagaa aatattcttg gcggtgtggg
    23701 taaagcgaca gcttatcttg cggtacaact gaaaaaaaca ccggaagcgg ctgctgagtt
    23761 tgctgcaaag atgcaggatg ctaccggaac ggcgtcagaa gacatgatgg ggctgttcga
    23821 cactatccag aaggcgtttt atctgggcgt tgacgatacc aacatgttgt ccttcttcac
    23881 taaaaccagt tctgttctga agatggtgaa caaggacggt cttcaggctg cacagagcct
    23941 tgcccccatc agcgtcatga tggatcagat ggggatgaac ggggagtcgg caggtaatgc
    24001 cctgcgaaaa gttatccagt ccggattaag cgttaagaaa atcagggacg tcaataaaat
    24061 catggcccgc cagaaactcg gggtacagct cgattttact gacggcaaag gaagttttgg
    24121 cggtcttgat aacatgttca ggcaactggc aaagctgcga aaactgaccg acgttaagcg
    24181 aacaggtgta cttaaggcaa tatttggtga tgatgccgaa acccttcagg tggtcaatgc
    24241 actaatcgat aaaggaaagg atggctacga tcagatccag cagaagatga ataaacaggc
    24301 cagcctgaat aaacgtgttc aggcccagct tggtacgctg tccaacctgt gggaggcaat
    24361 gacggggacc gcaactaacg gccttgcggc tattggcggc gcattttctg gtgacgccaa
    24421 aaatatcacg caatggctgg gggagttggg ggagaaattc acgaagtttg cggatgaaaa
    24481 tccccgggtt attcgcggcg tcgtcgggct tgctgccggt cttgcgattc tgaaactggg
    24541 attgatgggc gtgggcagtg ccatcagtat cgtcagcagg atcatgtcga tgacgccgat
    24601 tggcatgatt gcgacggcga ttgccctggc tgcgggatta attatcacta actgggatgt
    24661 tgtcggacct tattttaaga aactctggga aaccattagt ccttattttg aggctggctg
    24721 ggaacttctg aagaaggttt ttgcctggtc gccgctgggg atggtgatca ataactgggg
    24781 accggttgtt aagtggtttc aggatatgtg ggataagctg aagccaatta ttgagtggtt
    24841 taccgacagt tcaggtgaca cggtcgatgc cattaactcg gcgcagtggg gcgcgggtgc
    24901 ttatgatgct tatgggacgg gaataccggc gcggggatac ataccttatc cggcggtgga
    24961 tctggctcag tcaaacaacg cctccgatgc cacaggcccg aatcccttca tgattaacaa
    25021 agcttctgcg ccaaaagttg atggtgagat caaggtctct tttgtgaatt cgcctccggg
    25081 tatgcgggtt atggaaacgc gatccagcgg ttttgatgtc agccatgatg ttggctatac
    25141 gcgctttggc aggtaatgaa aaattaatct gttaatgagt cccactccgg tgggattttt
    25201 tatgtacgga gtttatatga cgtggaaaga cagacttcag gacgcgtcat ttcgcggtgt
    25261 gc
//
LOCUS       DAJTRQ010000046        25245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000046 DAJTRQ010000000
VERSION     DAJTRQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..892)
                     /gene="araF"
                     /locus_tag="OO332_001998"
     CDS             complement(<1..892)
                     /gene="araF"
                     /locus_tag="OO332_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR5942490.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKD"
     gene            1689..2192
                     /locus_tag="OO332_001999"
     CDS             1689..2192
                     /locus_tag="OO332_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5942491.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2272..2523)
                     /locus_tag="OO332_002000"
     CDS             complement(2272..2523)
                     /locus_tag="OO332_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5942492.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2638..2724)
                     /gene="azuC"
                     /locus_tag="OO332_002001"
     CDS             complement(2638..2724)
                     /gene="azuC"
                     /locus_tag="OO332_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5942493.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            2987..3310
                     /locus_tag="OO332_002002"
     CDS             2987..3310
                     /locus_tag="OO332_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5942494.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3481..3978
                     /gene="ftnA"
                     /locus_tag="OO332_002003"
     CDS             3481..3978
                     /gene="ftnA"
                     /locus_tag="OO332_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5942495.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4015..4254)
                     /locus_tag="OO332_002004"
     CDS             complement(4015..4254)
                     /locus_tag="OO332_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5942496.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4444..5655
                     /gene="tyrP"
                     /locus_tag="OO332_002005"
     CDS             4444..5655
                     /gene="tyrP"
                     /locus_tag="OO332_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5942497.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5717..6382)
                     /gene="yecA"
                     /locus_tag="OO332_002006"
     CDS             complement(5717..6382)
                     /gene="yecA"
                     /locus_tag="OO332_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5942498.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6578..6664)
                     /locus_tag="OO332_002007"
     tRNA            complement(6578..6664)
                     /locus_tag="OO332_002007"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6628..6630),aa:Leu,seq:taa)
     gene            complement(6677..6750)
                     /locus_tag="OO332_002008"
     tRNA            complement(6677..6750)
                     /locus_tag="OO332_002008"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6716..6718),aa:Cys,seq:gca)
     gene            complement(7032..7580)
                     /gene="pgsA"
                     /locus_tag="OO332_002009"
     CDS             complement(7032..7580)
                     /gene="pgsA"
                     /locus_tag="OO332_002009"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5942499.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7637..9469)
                     /gene="uvrC"
                     /locus_tag="OO332_002010"
     CDS             complement(7637..9469)
                     /gene="uvrC"
                     /locus_tag="OO332_002010"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5942500.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9466..10122)
                     /gene="uvrY"
                     /locus_tag="OO332_002011"
     CDS             complement(9466..10122)
                     /gene="uvrY"
                     /locus_tag="OO332_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5942501.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10418..10594
                     /locus_tag="OO332_002012"
     CDS             10418..10594
                     /locus_tag="OO332_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942502.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10581..10805
                     /locus_tag="OO332_002013"
     CDS             10581..10805
                     /locus_tag="OO332_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5942503.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(10838..11458)
                     /gene="sdiA"
                     /locus_tag="OO332_002014"
                     /pseudo
     CDS             complement(10838..11458)
                     /gene="sdiA"
                     /locus_tag="OO332_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11688..12440)
                     /gene="yecC"
                     /locus_tag="OO332_002015"
     CDS             complement(11688..12440)
                     /gene="yecC"
                     /locus_tag="OO332_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5942504.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12437..13105)
                     /gene="tcyL"
                     /locus_tag="OO332_002016"
     CDS             complement(12437..13105)
                     /gene="tcyL"
                     /locus_tag="OO332_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5942505.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13120..14106)
                     /gene="dcyD"
                     /locus_tag="OO332_002017"
     CDS             complement(13120..14106)
                     /gene="dcyD"
                     /locus_tag="OO332_002017"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5942506.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14211..15011)
                     /gene="tcyJ"
                     /locus_tag="OO332_002018"
     CDS             complement(14211..15011)
                     /gene="tcyJ"
                     /locus_tag="OO332_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5942507.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15099..15650)
                     /gene="fliZ"
                     /locus_tag="OO332_002019"
     CDS             complement(15099..15650)
                     /gene="fliZ"
                     /locus_tag="OO332_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5942508.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15696..16415)
                     /gene="fliA"
                     /locus_tag="OO332_002020"
     CDS             complement(15696..16415)
                     /gene="fliA"
                     /locus_tag="OO332_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5942509.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16580..18232)
                     /gene="fliC"
                     /locus_tag="OO332_002021"
     CDS             complement(16580..18232)
                     /gene="fliC"
                     /locus_tag="OO332_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5942510.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18498..19904
                     /gene="fliD"
                     /locus_tag="OO332_002022"
     CDS             18498..19904
                     /gene="fliD"
                     /locus_tag="OO332_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5942511.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            19929..20339
                     /gene="fliS"
                     /locus_tag="OO332_002023"
     CDS             19929..20339
                     /gene="fliS"
                     /locus_tag="OO332_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5942512.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20339..20704
                     /gene="fliT"
                     /locus_tag="OO332_002024"
     CDS             20339..20704
                     /gene="fliT"
                     /locus_tag="OO332_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5942513.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            20782..22269
                     /gene="amyA"
                     /locus_tag="OO332_002025"
     CDS             20782..22269
                     /gene="amyA"
                     /locus_tag="OO332_002025"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5942514.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22303..22716)
                     /locus_tag="OO332_002026"
     CDS             complement(22303..22716)
                     /locus_tag="OO332_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5942515.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            22903..24108
                     /gene="yedE"
                     /locus_tag="OO332_002027"
     CDS             22903..24108
                     /gene="yedE"
                     /locus_tag="OO332_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5942516.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24105..24338
                     /gene="yedF"
                     /locus_tag="OO332_002028"
     CDS             24105..24338
                     /gene="yedF"
                     /locus_tag="OO332_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5942517.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24445..25115
                     /locus_tag="OO332_002029"
                     /pseudo
     CDS             24445..25115
                     /locus_tag="OO332_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25155..>25245)
                     /locus_tag="OO332_002030"
     CDS             complement(25155..>25245)
                     /locus_tag="OO332_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HCR5942518.1"
                     /translation="GHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         6280 a         6446 c         6009 g         6510 t
ORIGIN      
        1 cgtcttttgc tacccagttg taaagcattt cgctggattt atagccatgt acgtccgggc
       61 ttggcagcag ggaaccgtag aagccggttg cctgtgcttt agacagttcg cttactgcat
      121 ccacaccgtt aataccaata ccgatgatat cggctgcttt aaagccctga ccttcggttg
      181 cacgtacgcc gccaagtacg gtgctgtcgt tcataccgac gatcagccaa tgtttaactt
      241 ccggatgttg aaccagcatt gagttggcag cgtcaaatgc ccccgggatg tcgttagatt
      301 tggtaggtac ctgataaatt tgtttttccg ggaatcctgc agctttcagt gcgtccatag
      361 aacccgttgt acggcggcgg gcggtatcca gttcgttggc ggtaatcgcc atcactgcgc
      421 tttctttgac atcccagcca cgtttctgca tctctttata cagttcctgg ccctgacgtt
      481 cgccaatttt agtcgccgcc atcatcacca gaggaacggt atccattggc ttacctttgg
      541 cgttaacaaa ctggtcatcc actgcaatga ctttcatatc gtagccacgc gctttcgcga
      601 cgatggcaga gccgagtttc gggtccggag tacaaataac gaaacctttt gcgccactgg
      661 ctgccaggct gtcgatcgcg ttcaatgttt tttcgccatc cggcacggca atcttaataa
      721 cctcaaaccc taaatccttc ccggctttat cggcaaactt ccattcggtc tggaaccacg
      781 gctcttccgg ttgcttcacc agaaaaccga gcttcaggtt ctccgccata gcggattgtg
      841 acataacggc tgccagacca atggctgcca gggctttagt aaatttgtgc atggttctct
      901 ccagctttag tgtcgttttg tgtagggcaa aaacgaatga cattcgttaa attaatcgga
      961 aaacaaagca ttacctttta tctaaaagat aagtgaccgt gttgacatag ttttagcgag
     1021 aaattaattc tccataggag agcaatatca catcgcagaa ttacagtgag aacgtgcata
     1081 aatttagcgg gaaaagacat aaggaaaagc caatttgtca gacaaattgt tgaatgcaca
     1141 gcagattaat ccataagatt agcctggaaa tccttgttgt cttcggtacc catgcgggat
     1201 gtcttctttt taaccagtca ataggccgca ttacctggcg ttgagttttt gaaatggtgt
     1261 aataaccaca actcaaagat gtggaaaatg cgcgtcattc atttcgccat taattatcac
     1321 tgtgctcatt aattaacaga acacgtataa tgagagccat ctcgcaaaaa tgaaaaaacg
     1381 ttttataaaa tcatcacttc atcatgaatt caaattcatc gattaatatc aacaagatac
     1441 aaaaagcact atcattaaaa ttcattgcat ttacattgat ttcaccaatg aaatgtaaaa
     1501 atatataaac ttgatgattt aagcattttc ttataaccgt tcagacgtta ttcttatttc
     1561 agatcatcgt cagaattgac tccacgatca catttcggac cggcagaaag gaattattct
     1621 gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga
     1681 tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt
     1741 tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg
     1801 gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt
     1861 ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttccaggtg
     1921 aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt
     1981 caagcacgct ggcgcagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca
     2041 atttcctgcg cgatctggaa aaagaacagc agcatgatgg cctgttgctg caaaccattc
     2101 ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc gaccaacatg
     2161 ttctgaatgt cgtgtcacac cagctgcatt gatcatacat cggcgctaat acattgcgcc
     2221 gatgaagttt ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt tttaatggga
     2281 ttcaccctgt ggggtaaact tgagttcaat cagcgcgatg gctttttgga ttgcccgcat
     2341 ggtgaccggg tctgcggcag cgggatgatg agtaaagtcg atattcttca gctgactgga
     2401 cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat
     2461 aaccaactgg caagctacca catcagaaac cagttcctga tcggcattca gcggctggga
     2521 catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcagc
     2581 gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta
     2641 tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact
     2701 tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtgttatt
     2761 gtctttgcgc ttataatatg acaaccatca caaaaatcaa tctttatgtg atacaaatca
     2821 cataaatacc cctttaatgt tataaaaatg ataatcaaaa aacagccccc ctatttctga
     2881 cacctacaga tggcaagaaa tagcgcctgc caggcatctt ttccggccat tgtcgcagcg
     2941 ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga gacttctgat
     3001 tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccagg cccatgtgag
     3061 cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt ttttgcatca
     3121 tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc agcaggaagg
     3181 ctatacccac gcgatcccct ttggtcagcc agtaggcaac tgtagccttt ttgcgggttc
     3241 cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg ccttccctgt
     3301 ctttctctaa atattttaat cagcgaggtg atcttcgctg attaaagaaa tagcggtaat
     3361 gcgttttatt cccattcgta tttttaataa ttgaagttta tattttacct tttgcaaata
     3421 ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc
     3481 atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct
     3541 tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc
     3601 gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac
     3661 ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat
     3721 tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat cacccagaaa
     3781 attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg
     3841 cagtggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa
     3901 ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc
     3961 ctcgacacac aaaactaatg ctctccggcg gcaggtttct gccgccgttt caaatcagtg
     4021 acggcaaatc ttactctgat cggtcgaaaa accttcttct gaaggtataa atttgccttt
     4081 tgctgccaga aacgctacca gctctcccgc tgtcatccct tctgccgagc acgtgtgaaa
     4141 acgtgcctgt tcaccaaaac gcgctttaat cgcagcttcc agactggcat gcgtatattg
     4201 ctcgcctgat tcaatcatca tatttaacac ttcatgaccg tgaatagagt ccatcgttcc
     4261 tcctcaaaaa aagcctagcg tagcgattac cgcttatgaa gactttgcgc cagcgcagga
     4321 ctgaatgctt ttattgtaca tttatattta cacaatatgt aacgtcgatt tgacgaagca
     4381 gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa
     4441 agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca
     4501 ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg
     4561 ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag
     4621 catgttccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt
     4681 caatggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc agcatacatc
     4741 agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg
     4801 gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgttggaaca
     4861 tcgctggtcg atttatttaa ccgttttctg ttcagcgcca aaattatttt tctggtggtg
     4921 atgctggtat tgctgctacc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa
     4981 caggggctgg cactgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc
     5041 atgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata
     5101 atcggtagtg ccatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc
     5161 attgattcaa caacctttat gggattgctg gctaatcatg ctggattaaa cgggctgtta
     5221 caggcgttac gcgaaatggt gccctctccg catgttgagc tggcggtgca tttatttgct
     5281 gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct
     5341 gatttgtttc agcgttcaaa taccgttggt ggacgattgc aaactggtgc aattaccttt
     5401 ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac
     5461 gccggtgtgg cgctggcggt actggcattg attatccctt cactattgac ctggcaaagc
     5521 agaaagcaca atcctcaggc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg
     5581 gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta
     5641 ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga
     5701 cacccaatgt gacctcttaa tgcaggcagc actgcttaaa tttcttacca ctgccgcacg
     5761 gacaaggatc gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt
     5821 cctgcggatg cgccatccag tatgcatgta aatcaagcgc cgccagtcga atggcatcta
     5881 cgctctcttc aaacgcttct ggcgacattt tctctacccg ctcgaagttt tcctcagtac
     5941 cgtgcagcgc aatcgcctcc agcgctggtt ttaaagaatc gggcaacgtt gaccagtcag
     6001 aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc
     6061 catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
     6121 ctgcggtatc ggccatatgt tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg
     6181 acgcccaacg cggcacatag tcagccccac cccacacggc aaccagccac tgttctggtt
     6241 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
     6301 ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt
     6361 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
     6421 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgccggtg attaaaaatt
     6481 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
     6541 actgcttatt actttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
     6601 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
     6661 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
     6721 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
     6781 gcttgacgcc gatgtctttt aaactggagc gggaaacgag actcgaactc gagaccccga
     6841 ccttggcaag gtcatgctct accaactgag ctattcccgt attcatcaag caatcagtta
     6901 atcacttgat tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac
     6961 ttacctggcg cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaatta
     7021 cgccgaaacg atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt
     7081 gaccacagag tcagtaccgc agccacaaag aaaagtgcaa taccggcgta ctcaacccaa
     7141 atgttcggac gccacagcag ccaggccaac gccaccatct gggcagtggt tttcactttc
     7201 ccaatccagg agacggccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc
     7261 gcagaaataa taatttcacg ggcgatcatc gttgccgccg gtaatgtcac ccaccagctg
     7321 tgataatgct cggttaccag caccatggcg atagccacga gaactttatc tgccacaggg
     7381 tcaaggaaag caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac
     7441 cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca
     7501 ggcagataaa agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac
     7561 gtagggatat taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta
     7621 tgttgctaca gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg
     7681 aaatacccgg cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc
     7741 ccatatattt caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg
     7801 aactggtatt tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcatgtg
     7861 attcatcgcg aatatgctgg ataacatgca gcgcgggtga atctggcggc aaactaaatc
     7921 cctcaccttc cggctcaaag aacagcgttt ccagtccagc cttacgatct gctcctttgg
     7981 caacgccaag tagcagcgga tgatttttat cccatgagac atccagttcg gcgaagacat
     8041 ttttcgcctg cgcaagctgg cctttgccgc cgtcgataag gatcacatcc gggatcttac
     8101 tgtcgtcaat ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat
     8161 cgcccggcgt gatgcctgta atgttatagc gccgatactc cgcacgcagc gggccgttag
     8221 catcaaacac cacacaggaa gcgacggttt gttcgcccat ggtatggctg atgtcaaagc
     8281 actccatccg cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt
     8341 gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga
     8401 gtttcagata acgcgcccta tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg
     8461 ccagttctga aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga
     8521 tctcacccgg taaggtgcgc atctggctgc cttgtaaata gaactgacct acgaaggttt
     8581 ctaccacctc gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccga
     8641 gcactttgcc ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca
     8701 caccaataac gtcaaggtcg tcgccggtat tggaaacaaa ctgtttttcg gtgacgcgtc
     8761 gcaccgcctg aatttggtcg cgaatacatg ccgcttcttc aaactccaga ttctggctgg
     8821 cagtttccat acggctaatg agttgcgtaa gcacctgatc atctttgcca gacaaaaaca
     8881 ggcgcacata ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg
     8941 gtcccagaca gcgccctatc tggtattgca gacacggacg cgagcgattg cgataaacac
     9001 tattttcgca ctggcgaatg gggaaaatct tttgcagtag cgccagtgtt tcacgtacgg
     9061 catagccatt cgggaacggg ccgaaatatt cacctttggc atgctttgca ccacgatgca
     9121 tcgccagacg cgggtgggta tcgccactca ggaagataaa aggataagat ttatcatcgc
     9181 gtagcaaaac gttgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg
     9241 cttcggtttc tgtatgcgta accgttacat caatttgctg gatctgggcg accagcgctt
     9301 cggttttgcg cgaagcgagg ttgctgcgga aatagctgga aagccgtttt ttcaggtctt
     9361 tcgctttgcc gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct
     9421 ggctggttac ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa
     9481 tgtctccgca ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg
     9541 aatgtttagt ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt
     9601 gagctgttct gagatctcat tgaccttctg gcccttagtg atcatcagca taatctgcaa
     9661 ttcacgttca gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa
     9721 cgccatttgt tgagcgatat cagaagcaat gtaacgctgc cctgaataga cagaacgaat
     9781 cgcactcacg acttcctgcg gagccgcgcc tttgctgagg tagccagcag cacctgcctg
     9841 catgactttc gctggtaaag ggttttctgt atggaccgta agcatgatga ttttaacatc
     9901 agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat
     9961 gtccattagc accacgtcaa cggcatttgt ccggcaccac ttaacggcgt cttcaccgca
    10021 cgatgcctct ccgacgactt ttataccctt tatatcttcc agaatgcgtc gtatccctgc
    10081 gcgcaccagt tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat
    10141 agggataacg ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga
    10201 aacgttaaaa atggtgggta ttcgattttt ctctcgtttt tgtcaattcg attgtccaca
    10261 gttatggtca cgcccgtcac caacggctta ccggatcgtt tcacagtaag ccctacgatg
    10321 aaatgtgaca aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa
    10381 cattaattaa cccgaacaat acgggcaaaa aacattcatg ataaaaatat ttattggtca
    10441 ttatattaac gtattttata gcactgccga tatcacgctc aaaaaacaac cactgctatt
    10501 tttagcaaag cttatggtat actccgccgc cttaacattt ttcaccgcaa attttcattg
    10561 caacatgacg aggaaaataa atgagtacgc ctgatttttc tactgccgag aataatcaag
    10621 aactggcaaa tgaagtctcc tgcctgaaag cgatgctgac gctgatgctg caggcgatgg
    10681 gacaagctga cgcgggccgc gtgatgttaa agatggaaaa acagcttgcg ctgatcgaag
    10741 acgaaaccca ggctgcagta ttttccaaaa cggttaagca aattaaacaa gcctaccgtc
    10801 agtaatataa aaccggctga gagcgtgcct ttcagccggt tattgacaaa atcatcgcta
    10861 tctctgctga tgtcttccct tccgccgtcc atttcagaat ttctttttcg cgcttgctga
    10921 aattcatctc tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt
    10981 cgcgcaccag taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgcg
    11041 tgctgcaacg ggaaaaggac aaaaagccca gcgcccgatt tggcagcatt aaatactgag
    11101 tgacaccgcg gcgtaaacca tgtgcgtgcg cggcttccca taatggctgt gcttcgctga
    11161 ataagtcatc attccacatt aaatggtcct gactaaagtt ttcagggttc agcacaggat
    11221 caattgcgag aaaatttttt gcctgataat aactaaccca cgactcaggg taattggtgt
    11281 agaaagccac tttaggtcgc gtgaatagta ccgggtggcg aacacataac gaatagtaat
    11341 cgtactccag ctgctgagcc tgaagctcta tttcatggta gacctcttct gcggcctcca
    11401 tcctctgaaa acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag
    11461 taaaccgcaa cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata
    11521 aataagattt atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcgc
    11581 attctcttat gttattaatg agttatgctg atttgttaag cagttttatc acgcttgaaa
    11641 tggcgtcccg ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga
    11701 agcttctcga ggaactggcg ggtgcgaggc tgctggggat cggcaaataa cgcttttgct
    11761 gccccctgct cgactatccg cccctggtcc ataaagatcg cccggtccgc aacatcccgg
    11821 gcaaagctca tttcgtgtgt cacaatcacc atcgtgcgct tttcctgcgc cagctggcgg
    11881 atggtgttca ggacttcgcc caccagctct ggatccagcg ccgacgttgg ctcgtcaaac
    11941 aaaatcacct caggacgcat tgccagtgca cgcgcaatcg caacacgctg ctgttgaccg
    12001 ccagacaaac gacgtggata gctggtttct ttaccggcca gcccaacttt cgccagcagc
    12061 tcgcgagcgc gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca
    12121 ataatgtttt ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg
    12181 acgtgctgac gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca
    12241 atagtgatat cgccaacggt gatcgttccc gcttccggtt gttccagcag atttatgcta
    12301 cgtagcaatg tggttttgcc ggaaccactc ggaccgataa ttgccaccac ttcgccaggc
    12361 tttacctcga ggtcgatacc gtgcagcacc gtctgaccgt ggaatttttt caccaggttc
    12421 ttaacttcaa tggcactcat tttggttctc tctcctggcg attaagttga ttctcaaaat
    12481 ggttctgcaa cgtcgataac acagtcgcca tgatccagta gatgagcgaa gccgccaggt
    12541 acatggtgaa cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg
    12601 gcacctggat tgtcgcggcc agcgacgtat cttttaccag actgataaag ctgttcgaca
    12661 gcggcggcaa cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcatg gtctgccacg
    12721 gcgtcatacc aatactggca gcggcttccc actgaccttt atcaatagaa gaaatggcgg
    12781 cgcgcagcgt ttcggcggca taggcggcag tgttcagcga cagaccaatc attgctgacg
    12841 gaatgggatc taattcgata ccaaactgcg gcaggccgta atagatcata aacagttgtg
    12901 cgatgagcgg cgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc
    12961 agattggcga caggcgcatc agcgcgagaa taaacccgag tagtaagcca aaaaacatgc
    13021 cgccgatact gagttgcagg gtatacccag cccctttcaa caggaacggc aaggaatcaa
    13081 taaccagttg tatactttct tgcatgagcg ttcccggagt taaacgtggg gatgataggc
    13141 gaacagcgca ggcgcgccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg
    13201 tttctgactg ataccgtcaa tcagccccgc catcgctttt ccggtataca caggatcaag
    13261 cagaatacct tccagacgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac
    13321 gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt tccgctgatg cggtcagctc
    13381 cagttctttc gcaatcgcct gttgtaggtt aaccactttc ggcaattgat cggcaacgga
    13441 acgcgacacg gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc
    13501 cagtccggcg tgagttccgg cactgcccga tgcgactacc accgacgaaa tattaaccgc
    13561 cccttcacac tgttgcgcga tttccagcgc actctccaca taacctagcg cgccaagagc
    13621 attagaaccg ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc
    13681 cagctcttcc agttgggcat tgggatcggt cagtgcgtcg cacatttcaa tctgggtatt
    13741 gaacagatcc agcaacaaac gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat
    13801 aggattttcc agcagcgcca cgcagtgcag accgagtttc gccgcgactg cggcagtctg
    13861 gcgcacatgg ttagactgga tcgcgccggc agtaatcagc gtatcggcac cttcgcgcag
    13921 ggcatctgcc gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggggt
    13981 gacctcatcc cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata
    14041 ttcgagcggc gttggcgcgc cgataaactc caaccgtgga aaacgggtta aattatgcag
    14101 tggcataaca gcctccgatg cgtgttgttg tgattttctt attatgcacg ctgaaaacgc
    14161 gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc attatgctga ttatttggtc
    14221 acatcagcac caaaccattt ttcggaaaga gcttgcagag tgccgtcttt ttgcatttcc
    14281 gcaattgcat cattcaccgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca
    14341 gactcctgac gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc
    14401 agatccagcg ccgccagacg atcaacgagg atcgcatcga tacgccctac gcgcagatcc
    14461 tgatatttgg tcgggtcatc atcataggta cgcacatcga caccctgaac attctgccgc
    14521 agccactctt catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca
    14581 gccgttttaa tggtgccttc gttacctttt ttcaccagcg cctgaatacc ggaaatggtg
    14641 taaggggtag agaaatcgta ttttttcttg cgctcgtcag aaatggtgac ctgattaatc
    14701 accacatcaa tgcgtttaga gtccagcgac gccagcatac cgtcccattt ggtcggtttt
    14761 agtgacgcct caacgccaag atgttttgcc agctgttgag caaattccac ttcaaaaccg
    14821 gttaatttgc cgtcatcccc ctgaaaactg aacggcggat aagtgccttc cagccctacc
    14881 agcagcgtgc cgcgctcttt gactttatta agcagacctt catctgcaaa acttttaacg
    14941 ctcatgcccg caaccagcgc cacgcccatc acacccatca atgcctgacg tcccagatgt
    15001 gctaatttca tattcacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt
    15061 gtaaacacaa aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc
    15121 aggctggagg atttctgcac aagtccggta caggtttggc gctgatagtg ttgatacttc
    15181 cgtaatgcga tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca
    15241 tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt gctcatgtag atgattaccc
    15301 aaacggcgca gacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca
    15361 aaaagaaatt tcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa
    15421 tcaccgcaaa atcggcacaa tgccgcccat gattgttgtt ccgtttgcca gcctttttca
    15481 tcaagcagcg tgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaact
    15541 aaggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat gggtctggct gtgtttaaag
    15601 tctttaagat agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc
    15661 tggtagtcaa agttaaagtg cggcatttac tgacgttata acttacccag tttagtgcgt
    15721 aaccgtttaa tggcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc
    15781 gcgccaatct ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg
    15841 cgctccggca acgtttcgat ggcttccatc acccgctggc gcagattact gtccagcagt
    15901 tgttgtagcg ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc
    15961 tcttcacgcc actcatcgta ggagaagagc tggctgttat tggtgtcgag cagcatttga
    16021 cgataatcgg caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg
    16081 ccaagttcct gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg
    16141 ctgcgcggca cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc
    16201 actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac agcattaagt
    16261 aacccaatgc cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc
    16321 tgcaggcgca atgcttcgtg acacaccagc gggacataac gctgccacag cgagtgttta
    16381 tccattacac cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag
    16441 ttatcggcat gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg
    16501 aggggttatt tgggggttac gcaaaaagcc gaatgtaaaa aaccccgtcg gtggcggggt
    16561 ttgagaggca ttcagacgat taaccctgca gcagagacag aacctgctgc ggtacctggt
    16621 tggcttttgc cagcacggag ttaccggcct gcaggataat ctgcgctttc gacatgttgg
    16681 acacttcggt cgcatagtcg gcgtcctgaa tacgggactg cgcttcagac aggttggtag
    16741 tggtgttgtt caggttggtg actgcggaat ccaaacggtt ttgcaccgca cccagggagg
    16801 agcggaattt gtcgatggag ctgatggctt catccagagc tttcagggga tcggctgtcg
    16861 tagctgcttt tgtttcagca tctgtcgtca atttaccatc agcatcttta taaactgcct
    16921 taccgctgcc gttagtcact gaaccatcat cctgtaggta aaaatctaca gtatcagatg
    16981 tggtcaacgc ctcgttgtta tcaacataaa ccgaatcaac accttttgtt acagcgccag
    17041 ttgtgccgtt gacgctataa cctacagtag tgaaatcaac attgtttccc gaggttgata
    17101 cagctttatc gaaagctgcc tgatcaactt ttgcacctgt tactgaataa gtaatagtgt
    17161 ccggcgtagt actattaccg gtaaaggaga tagaggtacc gccaatgtca atggttgtcg
    17221 ctttggtatt attggctgca agtgtggaca gactagcctc agtcaaagaa gcaccagagc
    17281 cgtttttcgt taagcgccca gttgtatcaa tgtagagttt atctccattg cttgccgtaa
    17341 ttttaccatc actgctgatc tgaacatcct gtgcagaacc atcgatagta atagtgccct
    17401 tagcggtatc accaactccc ggggtcaaat atttctgaac atcggcagct gaagccgttg
    17461 tggtatcaaa agagtatgac ttacttgcac tgtcatactt gtaattcgtt gcactagccg
    17521 ccgttccgaa gccgttatta atggttgcct gaacagtatc accatcacta actccagcca
    17581 gcaaatcaga cgctttagca gcatcgtaac ccgcactcac agtatatttg ttgccgacca
    17641 ctgttgcatt agcagctacc aagtcagctt tagatgcagc agtattagcc acagccccgc
    17701 caccattcac attaaaccca ttcagcccca gcgtatcaga gtcaattttc ttcagatcaa
    17761 tagtgatagt ctggccgtca ttcgcaccaa cctgaatttt catcgaaccg tcttttgcca
    17821 gtacgttcac gccgttgaac tgggtctgac cggatacgcg gtcaatttcg tcgagacggg
    17881 atttgatttc gtcctgaatg gagtccagat ccgaatcaga gttagtcccg gtagaagcct
    17941 gaaccgtcag ctcacggata cgctgtaagt tgttgttgat ttcggacagc gcgccttcag
    18001 tggtctgtgc aacagaaata ccgtcgttgg cgttacgtgc agcctgagtc aggcctttaa
    18061 tgttagaagt aaaacggtta gcaatcgcct gacccgcggc gtcatccttc gcgctgttaa
    18121 tacgcaagcc agaagacaga cgctcgatag aactcgacag cgcagactgg ttcttgttga
    18181 tattattttg agtgatcagc gagaggctgt tggtattaat gacttgtgcc atgattcgtt
    18241 atcctatatt gcaagtcgtt gattacgttt tgggtttcca cccgtcggct caatcgccgt
    18301 caaccctgtt atcgtctgtc gtaaaacaac ctttagaatt tttttcacaa acagccattt
    18361 tttgttagtc gacgaaatac tcttttctct gccccttatt cccgctatta aaaaaaacaa
    18421 ttaaacgtaa actttgcgca attcaggccg ataaccccgg tattcgtttt acgtgtcgaa
    18481 agataaaagg aaatcgcatg gcaagtattt catcgctggg agtcgggtca ggtctggatt
    18541 taagttcaat ccttgatagc ctcaccgccg cgcaaaaagc gacgctaacc cctatttcaa
    18601 atcagcaatc gtcgtttacc gctaaactta gcgcctacgg tacgctgaaa agcgcgctga
    18661 cgactttcca gaccgccaat actgcattgt ctaaagccga tcttttttcc gccaccagca
    18721 ccaccagcag caccaccgcg ttcagtgcca ccaccgcggg taacgccatc gccgggaaat
    18781 acaccatcag cgtcacccat ctggcgcagg cgcaaaccct gaccacgcgc accaccagag
    18841 atgatacgaa aacggcgatc gccaccagcg acagcaaact caccattcaa caaggcggcg
    18901 acaaagatcc gattaccatt gatatcagcg cggctaactc atcgttaagc gggatccgtg
    18961 atgccatcaa caacgcaaaa gcgggcgtaa gtgcgagcat cattaacgtg ggtaacggtg
    19021 aatatcgtct gtcagtcaca tcaaatgaca ccggccttga taatgcgatg acactctcgg
    19081 tcagcggtga tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg
    19141 gtatggaggt ctcggtcgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccatcg
    19201 agaacagcag caacactatc agcgacgcgc tggaaaacat caccctgaac ctgaacgatg
    19261 tcaccacggg caaccagacg ctaaccatca ctcaggacac ctccaaagcg caaacggcga
    19321 ttaaagactg ggtgaatgcc tataactcgc taatagatac cttcagcagc ctgaccaaat
    19381 acaccgccgt agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg
    19441 actccacgct gcggacgatt cagacgcagt tgaagtcgat gctgagtaat accgtcagtt
    19501 cttccagcta taaaacgctg gcgcagattg gtatcacgac cgatcccagc gatggcaaac
    19561 tggaactgga tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg
    19621 cattgattgt tggcgatggt aaaaaaaccg gcatcacaac caccatcggc agcaacctga
    19681 ccagttggct ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaaaaccc
    19741 tgaataagtt aactaaagac tacaacgccg ccagcgatcg cattgatgcg caggtcgcgc
    19801 gctacaaaga acaatttacc caactggacg ttttaatgac ctcgttaaac agcaccagca
    19861 gctacttaac gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga
    19921 gacaggtcat gtacgcggca aaaggcaccc aggcctatgc gcaaattggc gtcgaaagca
    19981 ccgtaatgag cgccagccaa cagcagttgg tcaccatgct atttgatgga gtgctgagcg
    20041 cactggttag agcgcgactg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt
    20101 tgtcaaaagc gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca
    20161 aagacgaatt aacccaaaac ttaattgctc tttatagcta tatggtcagg cgtttgctgc
    20221 aagccaattt acgcaacgat gtctccgcag tcgaagaggt ggaagcatta atgcgtaata
    20281 ttgccgatgc ctggaaagag tctttactct ccccttcttc gattcaggac ccagtctgat
    20341 gaacaatgca ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat
    20401 gttacgcctg gcaacggaag aacaatggga cgaactcatc gctagcgaaa tggcgtatgt
    20461 gaatgcggtg caggagatcg cacacctgac tgaagaggtt gccccgtcca ctacgatgca
    20521 ggagcagctc cgcccgatgc tgcacctgat tctcgacaac gaaagcaagg taaagcagtt
    20581 attacagatt cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc
    20641 ggtgttaagt gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt
    20701 ttgaatctta atgagccgat ggctcgcgaa taatccgatt acggctacgc ttctaatatt
    20761 ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat
    20821 tacccggaag gcggtaagct ctggcctgaa ctggccgagc gcgcagacgg ttttaatgat
    20881 attggtatca atatggtctg gttgccgccc gcctataaag gcgcatcggg cggctattcg
    20941 gtcggctacg actcctatga tttatttgat ctaggtgagt ttgatcagaa aggcagcatc
    21001 cctactaaat atggtgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat
    21061 gacattgcgg tgctgctgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa
    21121 gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt
    21181 gagtgtgaag gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcgcagttt
    21241 atctgggatt tcaaatgttt tagcggtatc gaccatatcg aacaccctga tgaagattgc
    21301 atttttaaaa ttgttaacga ctataccgga gaaggctgga acgatcaggt tgatgatgaa
    21361 ctcggtaatt tcgattatct gatgggtgag aatatcgatt ttcgcaatca tgccgtgacg
    21421 gaagagatta aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggctttcgt
    21481 cttgatgcgg tcaaacatat tccggcatgg ttttataaag agtggatcga acacgtacag
    21541 gaagttgcgc caaagccgct gtttattgtg gctgaatact ggtcgcatga agtcgataaa
    21601 ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag
    21661 atgaaattcc atgaagcatc gcgcatgggt cgcgactacg acatgacgca gatcttcact
    21721 gggacgctgg tggaagccga tccttttcac gccgtaaccc tcgttgccaa ccacgacacc
    21781 caaccgttgc aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgcc
    21841 ttaattttgt tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgca
    21901 cattacgaag atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa
    21961 cagcttgatg agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacattattt
    22021 ttcgaccatc cgaactgcat tgcctttagc cgcagtggca ccgacgaata tcccggctgc
    22081 gtggtggtca tgtcgaacgg agatgatggc gaaaaaacca ttcatctggg agagaattac
    22141 ggcaataaaa cctggcgtga ttttctgggg aaccggcaag agagcgtagt gacagacgaa
    22201 aacggcgaag cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag
    22261 gtgatttaaa tccatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg
    22321 taacggcgct ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat
    22381 cgtcatacca tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt
    22441 tacggtaacg ttagtgagat catcagacat caatgtcaac ttacccggca cggcaatccc
    22501 ccgctgccac tgacggcaat ccaaggtatc gccctcttta gtgacaatca ggctgcctat
    22561 cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca
    22621 gtccggcgcg ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag
    22681 caacatgagc gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa
    22741 gaggattgtg gcattaaagc tctgatgtcg ccagcctttc caggttcaga gagaaatttg
    22801 atctaactat tatttttgtg aattattacc ccgaaaataa atttgcatgg tgtatgattc
    22861 gcggctcttg tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc
    22921 aaacacgcct ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt
    22981 ctctccactt actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc
    23041 cgttggggcg gtcagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt
    23101 aaaattatcc atctggaagg atcgccatta acccgcatcg acggcatgat gatcctcggt
    23161 atgtttggcg gctgctttgc tgctgcgctg tgggccaaca atgtcaaact acgcatgcca
    23221 cgcagccgta tccgcattat gcaggccatc attggcggca ttatcgccgg ttttggcgcg
    23281 cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg gcattcctca gttctcgctg
    23341 catgcctggt tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc
    23401 ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg
    23461 acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc
    23521 atgctgggct gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg
    23581 tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc
    23641 cgcgatatgt ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg
    23701 gtgagtgcca tcgggatctt cagttacgta cagttaggcg ttgaacctaa aatcatgtgg
    23761 gcgggaccaa atgcggtaat tggtggttta ctttttggtt ttggcatcgt gctggcaggc
    23821 ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc
    23881 ggtctgggca acgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg
    23941 gcgctggcca ccgactggga caaaatcaac ctgctgaaaa cttttggtcc gatgggaggc
    24001 ctgctggtga catatttgct gctgtttgct gcgctgatgt tgattatcgg ctgggaaaaa
    24061 cgcttcttcc gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc
    24121 ctgattaccg ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg
    24181 aggcgatgcc gcaattaaaa aaaggtgaga tcctagaagt ggtgagcgac tgtccgcagt
    24241 cgatcaataa tattccactg gatgcgcgta atcacggcta cactgtgctg gatattcagc
    24301 aagacgggcc gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc
    24361 acagcgggaa catcgttacc tctgacacat agattttacc cgccccctct cctattcttt
    24421 tcagtatgat ctcaggaggt tgctatgtgt ggacgctgtg cccaatccca aacgcgtgag
    24481 gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc
    24541 attggcagat acaatgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa
    24601 caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg
    24661 ctgattaacg cccgcgtaga aactgcctca accagtcgca tgtttaaacc gctctggcaa
    24721 catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa
    24781 aaaagcctta ttttatctat cgcgctgacg gacaacctgt ttttatggcc gcgataggta
    24841 gcataccgtt tgagcgtggt gacgaagccg aaggattttt gatcgtcact gcggcggcag
    24901 atcaaggtct ggtagatatt catgaccgcc gcccgctggt tctgtcgcca gaagctgcac
    24961 gggaatggat gcggcaagac attggtggta aagaagcctc agaaatcgct accagaagct
    25021 gtgtgccagc aaaccagttc acctggcacc ctgtgtcgcg cacggtgggt aatgtcaaaa
    25081 accagggcgc ggagttaatt caacctgttt gttgacatcc tccacgccct gaatgacgag
    25141 gccccctgct acgttcaggc tgtcgcctga atcatttcgg tgggttcctg cttcaacgag
    25201 cggcctgact gcaccatctc tccacaggca agaacggcgt gcccc
//
LOCUS       DAJTRQ010000047        24801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000047 DAJTRQ010000000
VERSION     DAJTRQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(51..884)
                     /gene="fdhD"
                     /locus_tag="OO332_002031"
     CDS             complement(51..884)
                     /gene="fdhD"
                     /locus_tag="OO332_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5942519.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1078..4128
                     /gene="fdnG"
                     /locus_tag="OO332_002032"
     CDS             1078..4128
                     /gene="fdnG"
                     /locus_tag="OO332_002032"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1663..1665,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5942520.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4141..5043
                     /gene="fdoH"
                     /locus_tag="OO332_002033"
     CDS             4141..5043
                     /gene="fdoH"
                     /locus_tag="OO332_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5942521.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5040..5675
                     /gene="fdoI"
                     /locus_tag="OO332_002034"
     CDS             5040..5675
                     /gene="fdoI"
                     /locus_tag="OO332_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5942522.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5672..6601
                     /gene="fdhE"
                     /locus_tag="OO332_002035"
     CDS             5672..6601
                     /gene="fdhE"
                     /locus_tag="OO332_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5942523.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6931..7173)
                     /locus_tag="OO332_002036"
     CDS             complement(6931..7173)
                     /locus_tag="OO332_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942524.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7391..7609)
                     /locus_tag="OO332_002037"
     CDS             complement(7391..7609)
                     /locus_tag="OO332_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5942525.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8288..9421)
                     /locus_tag="OO332_002038"
     CDS             complement(8288..9421)
                     /locus_tag="OO332_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942526.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9591..9929)
                     /locus_tag="OO332_002039"
     CDS             complement(9591..9929)
                     /locus_tag="OO332_002039"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942527.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="OO332_002040"
                     /gene_synonym="yiiD"
     CDS             complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="OO332_002040"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5942528.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="OO332_002041"
     CDS             complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="OO332_002041"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5942529.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11939..12811)
                     /locus_tag="OO332_002042"
     CDS             complement(11939..12811)
                     /locus_tag="OO332_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5942530.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="OO332_002043"
     CDS             complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="OO332_002043"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5942531.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13503..14287)
                     /locus_tag="OO332_002044"
                     /pseudo
     CDS             complement(13503..14287)
                     /locus_tag="OO332_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(14321..15217)
                     /gene="yihV"
                     /locus_tag="OO332_002045"
     CDS             complement(14321..15217)
                     /gene="yihV"
                     /locus_tag="OO332_002045"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5942532.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            16150..17040
                     /gene="yihT"
                     /locus_tag="OO332_002046"
     CDS             16150..17040
                     /gene="yihT"
                     /locus_tag="OO332_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5942533.1"
                     /translation="MPLGAASREVYSQARASGGFAMLAVDQREAMRMMFAAAGAPAPV
                     ADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNG
                     IPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVS
                     IIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRL
                     NDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDV
                     CAPKLQQLGDIVDEMMAKRR"
     gene            17057..18298
                     /gene="yihS"
                     /locus_tag="OO332_002047"
     CDS             17057..18298
                     /gene="yihS"
                     /locus_tag="OO332_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5942534.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18436..19338
                     /locus_tag="OO332_002048"
     CDS             18436..19338
                     /locus_tag="OO332_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5942535.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19537..21573
                     /gene="yihQ"
                     /locus_tag="OO332_002049"
                     /pseudo
     CDS             19537..21573
                     /gene="yihQ"
                     /locus_tag="OO332_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21619..23004
                     /locus_tag="OO332_002050"
     CDS             21619..23004
                     /locus_tag="OO332_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5942536.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23047..24450
                     /locus_tag="OO332_002051"
     CDS             23047..24450
                     /locus_tag="OO332_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5942537.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24518..>24801
                     /gene="ompL"
                     /locus_tag="OO332_002052"
     CDS             24518..>24801
                     /gene="ompL"
                     /locus_tag="OO332_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5942538.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGL"
BASE COUNT         6140 a         6380 c         6091 g         6190 t
ORIGIN      
        1 cgaaatgaat aagtcctgca atttaatata tatcgcagga cttattttaa ttaattgctc
       61 aaacgctgcg gatgggtata aaccgttgcc cgacccggtt tacaaaaacc taccagtgtc
      121 agattacagc gctcagccac ttctacagca agcgtggtcg cggcagacac cgcaaacaaa
      181 atctctacgc cgcacatcgc cgacttttgc accatttcat aactggcacg actggaaacc
      241 agtaccgcac cttgctgcca gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc
      301 agcgccacat ggcgacccac gtcttcatgc ccgccgacca gttcgccaga tggcaacatc
      361 caggcagcgg cgtgagtaca accagtcagt tgccccactg gctgaaaatc gttgagatga
      421 cgtaatgcat catccagttt gttgagatca aacgtctggg tgaacggtag cggctgcacc
      481 ggttttccga tgtcattaag ttgctccacg ccgcatacgc cacagcccgt acgtcccgcc
      541 agcgcccggc ggcgctcctt caaccccata aagcggcggc tggaaagctc aatttgtact
      601 tcaagaccat tacaggaagg aacgacatcc atgccgaaga tatcgcgcgg actttctatt
      661 atcccctcgg aaagcgaaaa accgagcgca aagtactcaa ggtctttggg cgacgccatc
      721 atcaccacat gcgaaatgcc gttgtagacc agcgcaacgg gaacttcttc cgcgacctca
      781 tctaaccgtg ggtgttgtaa atcatcacgc cgccataact caatttgacg aacacctgtg
      841 atatttgtca cattttcgat ttcttttcgc tgtgtttttt tcactttctc ttaaccagat
      901 aagaacacac acaccaacat tatggtattc tgttacaaac ccttcctaga tggagggaaa
      961 ttgagccaat tctggacctt tgcggcccct tccgcaaaga aaaataactc ccactccctg
     1021 cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc gaaacaagga gcaatccatg
     1081 caggtcagca gaaggcagtt ctttaagatc tgcgctggcg gtatggcagg caccacggca
     1141 gcggcactgg gttttgcacc cagcgtagca ctcgcggaaa cccggcagta taaactgctg
     1201 cgcacccgcg aaacccgtaa tacctgcacc tattgttccg taggctgtgg gctgttgatg
     1261 ttcagcctcg gtgacggagc aaaaaacgcc aaagcatcta tcttccatat cgaaggcgat
     1321 ccggatcacc cggtcaaccg cggtgcactt tgtccgaaag gcgctggcct ggtggatttc
     1381 atccactccg aaagccgtct gaagtttccg gaataccgtg cgccaggttc tgataaatgg
     1441 cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc
     1501 gatgctaact acattgcgca aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc
     1561 gggatgctgt gtgcttccgc gtcgagtaac gaaaccggct atttaacgca aaaattctcc
     1621 cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg tctgacacgg accaacggta
     1681 gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt cgacatcaag
     1741 aacgccaacc tcgtcgtggt gatgggcggt aacgccgctg aagctcaccc ggtcgggttc
     1801 cgctgggcga tggaagccaa aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc
     1861 tttacgcgta cggcggcggt agctgactac tatgccccta ttcgttccgg tactgacatt
     1921 gctttcctgt caggcgtatt gctgtacctg ctgaacaatg aaaaattcaa ccacgaatac
     1981 accgaagcct ataccaacgc cagcctgatc gtgcgtgagg attacggctt tgaagatggc
     2041 ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata aatcctcctg gacttatgaa
     2101 ctggacgaaa acggcttcgc caaacgcgat accacgctgc aacatccgcg ctgcgtgtgg
     2161 aacttgctga aacagcacgt ttcccgctac acgccagatg tggttgaaaa catctgtggt
     2221 acgccaaaag acgcgttcct gaaagtctgc gaatacatcg cagaaaccag tgctcacgat
     2281 aaaactgcct cgttcctgta tgccctcggc tggacgcaac actccgttgg tgcgcaaaac
     2341 attcgtacga tggcgatgat ccagctgctg ctcggcaata tggggatggc cggcggcggc
     2401 gttaacgccc tgcgcggtca ctccaatatt caggggctga cggacctggg gctactgtcg
     2461 cagagtctgc caggttacat gacgctgcca agcgagaagc agaccgatct gcaaacctac
     2521 cttaccgcca acacgccaaa accgctgctg gaaggccagg taaactactg gggcaactac
     2581 ccgaaattct tcgtctctat gatgaaggcc ttctttggtg ataaagcgac ggcggaaaat
     2641 agctggggct ttgactggtt gccgaagtgg gataaaggct acgacgtcct gcaatacttc
     2701 gagatgatga aagagggcaa ggtcaatggc tatatctgcc agggctttaa ccctgttgcc
     2761 tcattcccga acaaaaacaa agtgatcggt tgtctgtcga aactgaagtt cctcgtcacc
     2821 atcgacccac tgaacactga aacctctaac ttctggcaga accacggtga gctgaacgaa
     2881 gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc catcgacctg cttcgcagaa
     2941 gagaacggtt caatcgttaa ctcaggtcgc tggttgcagt ggcactggaa aggtgcggac
     3001 gccccgggga ttacgctgac tgacggtgag atcctctccg gtatcttcct gcgcttgcgc
     3061 aagatgtatg ccgaacaggg tggcgcgaac ccggatcagg tgctgaacat gacctggaac
     3121 tacgccattc cgcatgagcc gaagtcggaa gaagtagcga tggagagcaa cggtaaggcg
     3181 ctggccgata ttaccgatcc ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt
     3241 agctcgttcg cccaattgcg tgatgacggt acaacctcct gtggctgctg gattttcgcc
     3301 ggtagctgga cgccggaagg caaccagatg gcgcgtcgtg ataacgccga tccgtctggc
     3361 ctcggtaaca cgctgggctg ggcatgggca tggccgctta accgccgcat tctgtataac
     3421 cgcgcctccg cagatccgca gggtaacccg tgggatccga agcgtcagtt gctgaaatgg
     3481 gacggcacta agtggaccgg ctgggatatt ccggactaca gcgcagcgcc tccgggtagc
     3541 ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc gtctgtttgc cctcgataag
     3601 atggcggaag gtccgttccc ggaacactac gagccgtttg aaacgccgct gggaactaac
     3661 ccgctgcatc caaacgttat ctcgaacccg gctgcacgta tctttaaaga cgacgccgaa
     3721 gcattgggta aagccgataa gttcccgtat gtcggaacca cctatcgtct gaccgagcac
     3781 ttccactact ggaccaaaca cgcgctgttg aacgcgattt tgcagccaga gcagtttgtg
     3841 gaaatcgggg agtcgctggc gaataaactt ggcattgccc agggcgatac cgtgaaagtc
     3901 tcctccaacc gtggctatat caaagccaaa gcggtggtga ccaaacgtat tcgcacgctg
     3961 aaagcgaacg gcaaagatat cgataccatc ggtattccga ttcactgggg ctatgaaggc
     4021 gtagctaaaa aaggctttat tgccaatact ttaacgccat tcgttggtga tgcgaacacg
     4081 cagacgccgg agtttaagtc cttccttgtg aatgtggaaa aggtgtaacg gagacgacat
     4141 atggcttatc aatctcaaga tatcattcgt cgttccgcga ctaacggtct gacccccgcg
     4201 cctcaggcgc gggacttcca ggaagaagtg gcgaaactca tcgacgttac cacctgtatc
     4261 ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg acattcgcga taccgtcggc
     4321 aataacattg gggtgtacga caaccccaat gatttaagcg ccaaatcgtg gacggtaatg
     4381 cgcttctcgg aagtggagca gaacgacaaa ctggaatggc tgatccgcaa agacggctgt
     4441 atgcactgtt ccgatccagg ctgcctgaaa gcgtgcccgg cggaaggtgc aattattcag
     4501 tatgccaacg gtatcgtcga cttccagtcc gagcagtgca ttggctgcgg ttattgcatt
     4561 gcgggctgtc cgttcgacat tccgcgcctc aacccggaag acaaccgcgt ctacaaatgt
     4621 acgctgtgcg ttgaccgcgt ggtggttggg caagaaccag cctgcgtgaa gacctgccca
     4681 acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtt
     4741 gctgagctga aaacccgcgg ttacgacaat gcgggtctgt acgatccggc aggcgtcggt
     4801 ggtacacacg tcatgtacgt gctgcaccat gctgacaagc caaatctgta tcatggcttg
     4861 ccggagaacc cggaaatcag cgaaaccgtg aaattctgga aaggcatctg gaaaccgctc
     4921 gcggctgttg gctttgcggc tacctttgcg gccagtatct tccactacgt gggtgtcggt
     4981 ccgaaccgtg cggatgagga agagaataat ctgcacgaag agaaagacga ggagcgcaaa
     5041 tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga acgtatcaac cactggatca
     5101 ccgccttctg cttcatcctg gcggcggtga gcgggctggg ctttttgttc ccgtccttca
     5161 actggttgat gcaaatcatg ggcacaccgc agctggcgcg aattctgcac ccgtttgtcg
     5221 gcgtggttat gtttgcctcg ttcatcatca tgtttttccg ttactggcat caaaacctaa
     5281 tcaatcggga tgatatcttt tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag
     5341 taggtgacac cgggcgttat aacttcggtc agaaatgcgt tttctgggcg gcgattattt
     5401 tcctggttct gctgctggtg agcggcgtga ttatctggcg tccttatttt gcgcctgctt
     5461 tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa
     5521 ttgtggttat catggtgcat atctacgccg ccctttgggt gaaaggcacg attaccgcca
     5581 tggtggaagg atgggttacc agcgcctggg cgaagaaaca tcacccgcgc tggtaccgtg
     5641 aggtccgcaa gacaacggaa aagaaagctg aatgagtatt cgcataatcc cgcaagatga
     5701 gctgggttcg agcgagaaac gtacggcgaa tatgattccg ccgttattgt tccctcggct
     5761 caagaattta tacaaccgcc gcgccgagcg tctgcgcgag ctggcagaaa ataatccgct
     5821 gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga
     5881 ccatccgctg gagatggatc tgaccgcgcg cattaaagaa gccagcgcac agggcaagcc
     5941 cccgctggat attcacgttc tgccgcgtga taagcactgg caaaagctgc tgatggcgct
     6001 aattgctgag ctgaaacctg aaatgagcgg cccggcgctg gcagtgattg agaatctgga
     6061 gaaggcatcg actcaggagc tggaagatat ggccagcgcg ctgtttgcct ctgatttctc
     6121 gtccgtcagc agcgataaag cgccgtttat ctgggctgcg ctgtcgctct actgggcgca
     6181 gatggccaat ctgatccccg gcaaagcccg cgctgaatac ggcgaacaac gtcaatattg
     6241 cccggtatgt ggctctatgc cggtgtccag catggtgcaa attggcacca ctcagggtct
     6301 gcgttacctg cactgcaacc tgtgtgaaac cgaatggcac gtagtgcgcg taaaatgcag
     6361 caactgcgag cagagcggca aactgcatta ctggtcgctg gatgacgaac aggccgcgat
     6421 taaagccgaa agctgcgatg actgcggcac ttacctgaag attctttatc aggaaaaaga
     6481 accgaaagtt gaagccgtgg cagatgacct cgcctctctg gtactggacg cgcgaatgga
     6541 gcaagaaggc tacgcccgta gttccattaa cccgttcctg tttccgggtg aaggagagta
     6601 atctctgacg ttttccgggt ggcgcaggtc gcccggatta cagctcatag ggtgtgacga
     6661 caccatctct cgtattccgc gttacccatt caagatcatg ccgcaattgt ctgtttgcct
     6721 cgttcttccc tccaacacat cgataaggtt atgggtatgt cactgatgac aagcgtatga
     6781 ttgcctgatg cgctgcgctt atcaggccta ccaataaact tcaatatatt gaatttgcat
     6841 gactttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg cgcacgttgt
     6901 cagcaatctg attgacatca ttttttccca ttaccattcc tcgcgcagct tacgctgata
     6961 ttcgacacca tcttcctcac acccttgcca gatgccagga acactggttc ttgctgtttg
     7021 cgattgattt atcaggtatt gatctaccgc ttcccttaat aggtccgcac gaggaagatt
     7081 acgctgcacc tcgagatcat caagttgctt aatcacctca ttcgataaat cgagtaaaat
     7141 tctgcccata tctatacctg ccagagagtt cataaatatc tcgccaatat catttttgaa
     7201 tctatggaga gaaaagtacc cttgccgaat ctttaaagaa agctcattta cgcatcattt
     7261 tttatttttg cgaggcgttt tccagatacg atgtaacagt tgcagttatt ataagttcaa
     7321 gtaaaacagc cttgtattaa taccagaata agggcatctg ttttttatat tcaagaatga
     7381 aaaatttttt tcattcctta tgctccttac actgatcatc aacaccaact acctccccct
     7441 tttgcctgcc tccctttgct ttacgcaccg gaaattgctg ccgctccaga tattgctcaa
     7501 ccgcttcgcg taaaattgca gatcgtgaaa ccttacgcca gccccgaagc ttgttcagcc
     7561 gtttaattgc ctcttctgat aaatgaagaa aaaccgtatt catcgccatt accaccacct
     7621 tacatatatt actcattaat gtatgtgcga aaattacgcc gaaaaagcat ttaaaaaaat
     7681 gcggattttt gcatgtcatt ttgtttttgc gaggcgtttt ccagaaataa taagagcgtt
     7741 gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg agttatgggt
     7801 gctatgaata tattatattc acggatattt atttgcataa tctgaatggt gtagccgaaa
     7861 aacgtcagca tcctaacagc accaggattt aaggtgaaat taatctttca tcacagaccg
     7921 gtcagtactg ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt
     7981 tcgggaaaca aggaattcct tccggaacac ccccggcatc atccgatagc attgacgtca
     8041 atcatcagcg cgacgatgag tttttttaat cgcatcatag ggtggcgacg ttcagcacga
     8101 ttacgggtca tagcaaaact cctgagcagg cggccactat gccgctttca ctggagttct
     8161 gaatactggt actttctgta tggcttcgcc atgcccctcg cggctaagta cctgcacaca
     8221 tctctctttg tatgtcattt ttgcaatgca ttgtccgaat tctgataatc ctaatgagtt
     8281 ttactcgtca gactcccatc cctacggtat acgagttata ttcatgaatg aaatcgacat
     8341 ttttaaaaaa cgaatcttgt aaaacatcag tgttgatatt accttgcaat ccaacattga
     8401 atttgttaga gacatgaaga actcgaatat caaaaaggtt ttttaacatc attaattctt
     8461 cgggtgttaa tgaatcgcaa ctaaaatatc cctgttgatc aaaaagattt tctatttctc
     8521 cactatcggc gaggcgtttt tcaaggagtg tttgcaacat cgagttgcca aggacaatgg
     8581 atatcgtgcg atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct
     8641 caagagtttc atttatcgcc gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat
     8701 ttctttcgtg atttaattta tctaccagtt cattccattt atcaaaagcc gggctatatt
     8761 tgttgcgcac agaaatatcc gactggttaa acaattcagc catgcgattt tttatcatct
     8821 cgatatccgg cgaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca
     8881 ttaatgccaa gcccacaact gtcgccatga tgggcgcagc cacggttttg gcaagggcga
     8941 cgctgcgatg gctatgcgct tcacaggcta aaggaacata ttccactgac tgcggtaatt
     9001 ccttacatgc ctccctcatc attgcggtat gctttacaaa caacggtttt tcactgccca
     9061 aaaaaacttt ctgcttaatt gcactaagaa aacgcaaatc aaaaataatt gtttttggtt
     9121 tattattttt tggcacattt cctattatcc cgctaagaac cttccttgct gcatggagat
     9181 ttttttgcac agcacataac acaattgatg aagccttctc cactgcatta ttgattttaa
     9241 ttttaaaatc cgcatgatat acgttgacgg gcaatgcaag tatatctgga cctacatctt
     9301 ttgctgagta tttattggtg atcgacggac gtgaacccac gagaaaactt agctgcaaat
     9361 tatgctctat attatcagct gtttttgtgg tgtggttaaa catagagatg ccacctttca
     9421 ttctttcaca catatcctca actttttcac ttcgttcttg tggcaatttg gctttaacat
     9481 taatttgatg atttttaccc gtgcattcct taactacaag gggaatgtta tctaaagtgc
     9541 catacaggct aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatacttg
     9601 aagcattcac aaagagggtc aatattataa ctgtttataa cttcacctac atcaagtgtg
     9661 tagtgcttac tctggagact gccatcctta tttaattgta tatcaatcaa tatatccccg
     9721 gactttgtca ccatatcttt gccataaacc atttgcatag ccatttcgag gagattttta
     9781 tctaactgat tgcgggcgtt gagtaagcga ttaccaaata atttatatac tgttcccgtc
     9841 attgccagcg ctgcgctttc tggtactttg cgtttccaca gtgaggtttc tctattttta
     9901 aacaggtcat attcattatt actcgacaaa ccatgcttca gttcgttaac acaatcgcac
     9961 agttgctcct caccttccat tttaaggcaa agtgaaaatt gatttaatgg cgtttacttc
    10021 ggtgatttat tactcattag ttgtttttct aaattaacaa cagaataact ttcttgttta
    10081 atgagattaa tattggcaac cattatagcc tccttcccac ggtttaagag caaacataca
    10141 acataatgat tcataacata cagagcagta aatgaatgat actgacaatg tatgccagcc
    10201 cattatcttt gcataatgtt tgtgagctat agattttaaa aatatttatg aaaaaagtat
    10261 ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt
    10321 aagcattatc tgcaataatt gccactcagc tctacaaata atatttaaat gctcctgaac
    10381 agtgatgtag caagacacgt atggtgccaa cgaagaataa aaatttataa agttcttatt
    10441 caatataacc ggaatagtca gggactgatg taaatgaccg gaatgaatga gagaggcagc
    10501 accgcccctc tccataagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg
    10561 cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa
    10621 gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc
    10681 gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg
    10741 gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcacgca gcatcagcca
    10801 gataagcccc caaccggtga gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg
    10861 gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg
    10921 ttgaatacgc acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag
    10981 ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gcttaatcat
    11041 caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tctccctgat taacaaaccc
    11101 cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc
    11161 ttcctgacgc gccaccgact ccagggtcat cgccatcagc gttcctaacc ctttgtcctg
    11221 cacgtccggg tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacaa
    11281 tcggcctacc gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc
    11341 atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca
    11401 gcgaaactga tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat
    11461 agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag
    11521 aatgtcacgg ggccatcatt gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt
    11581 tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg
    11641 cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg
    11701 agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc
    11761 ttgccttcgg catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct
    11821 ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg
    11881 cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc
    11941 atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag
    12001 tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga
    12061 gaatggggat caccgccagc acaccgtaaa tgagctgata tgacgggaac atggtgatat
    12121 aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa
    12181 tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga
    12241 tccacgacaa cagcaacgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc
    12301 tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg
    12361 gccccagcgt taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt
    12421 tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat aacgcggtga
    12481 cgatcagccc gcacgcccca acggcggtca tcttgttgga attagcaaca aattgttcga
    12541 tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta
    12601 actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa
    12661 ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt
    12721 tgtcctcatc aatacgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt
    12781 gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt
    12841 ggttttatct ttcaccagaa tactggtaat gcctagctga ttagctcctt ctatattatc
    12901 ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg
    12961 ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc
    13021 agcagcatca cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt
    13081 attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac
    13141 ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag
    13201 cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg
    13261 ctcatgctga tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat
    13321 acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt
    13381 acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta
    13441 gcgggaaagg atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa
    13501 atctacgcgt cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt
    13561 agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt
    13621 tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg
    13681 tgcgccatca tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca
    13741 tcgctctcaa tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag
    13801 gacgccgctg aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc
    13861 atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt
    13921 atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg
    13981 gtgataaata ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt
    14041 ttttctcggt ttgcgaaacc tcacgctgct cgaacgccgt attaacgacg ctggaagccc
    14101 gacccgcgcc accgtgatgg cgcgtaatca ggccttgctc gcttaattta cgaatatccc
    14161 ggcggaccgt ctgcgtggag acatccagca gatttgccag ctcatcaata ttcatatacc
    14221 cacgctcggc gatcagcatg aggagttggt cgtgccgcgg gttaccggtt agttcggtaa
    14281 ggctcatgaa aattcctcga aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa
    14341 aagatcgggt ttgatcacag tcagggatcc cggctcgtcc accgggacgt gtgcatttta
    14401 acgccgctac accgctggcg aagcggactg actccgctaa atccccactt gttgccagcg
    14461 ccaccgccaa agcgccgtga aaaacatcac ccgcacctgt ggtatctacc acatcaactt
    14521 tgaaggccgg ttgatgctga cgcccaccat tttccagcca gtcgcagcct gcgctaccct
    14581 gggtcacata gacatgacca tttgtgagcg tttgtgcctg ttttagcgca ctggccatct
    14641 ctttcacgcc cgttaagcgc gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg
    14701 ccaccagctc actgatatcc tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg
    14761 cctgacgggc cagggtgaag gcttttttaa cgccgtcgtg ccagcgtaca tctgccagca
    14821 caacatccca ctgagagaaa tcaatttcct ccaaccactc tgcgtcaggc agcagatccg
    14881 ggctggggta gttaatgatt atccgctcgc ctttggtatc caccatgatg gcggattgcg
    14941 aagatttcgc ctggttatac cgtttggtgt aacgggtgtt aacgccccag gattccagct
    15001 ctgctagcag gctgttgccc gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg
    15061 cccccagcct tgccgccgca accgctgccg tcgccgctgg cccgccacca acttccgtat
    15121 aatttctcgc cacgtattta ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat
    15181 ccatcacggt tatacctaca caagcaacac gaatcatggt cattccttaa acattttggg
    15241 atgcgttcat tttcatttca ccaattgttt aaaaagtgac ggctgtcaat tttttgacga
    15301 taaattacaa atgcgatcaa aaacagacaa gcaaagcatg tcataaatga caaaaagtga
    15361 catcactgta ttcaggagag ggttatggca gcaatcgcgt ttatcggttt aggacaaatg
    15421 ggttcgccaa tggcgagcaa tttattgcag caagggcatc aacttcgcgt ctttgatgtg
    15481 aacgccgaag ctgtgcggca tctggtagac aaaggtgcga ctcccgccgc caacccggtg
    15541 caggccgcta aagatgccga atttatcatt accatgttgc cgaatggcga tctggtgcgc
    15601 aacgtgttgt tcggtgaaaa cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt
    15661 gatatgtcca ccatccatcc gctgcaaacc gataaattga ttgccgatat gcaagccaaa
    15721 ggcttcagca tgatggatgt tccggtaggc cgtacttctg caaatgccat taccggtact
    15781 ctgttactgc tggctggcgg caccgctgaa caagttgaac gtgccacacc gatcctgatg
    15841 gcgatgggca gtgagttgat caacgctggc ggtccgggca tggggatccg cgttaagctc
    15901 atcaacaact acatgagcat cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc
    15961 gaagccctga atcttccctt cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt
    16021 aaaggtcact tcaccacttc ctggccaaac aaagtcctca gcggagatct ttctcccgcc
    16081 ttcatgatcg atcttgccca taaggatctt ggcatcgccc ttgatgtcgc caaccagctg
    16141 catgtgccaa tgccgctggg ggccgcctca cgggaggttt atagccaggc gcgcgcatcg
    16201 ggcggttttg ccatgctggc ggtcgatcag cgcgaagcca tgcgcatgat gtttgccgcg
    16261 gctggggcac ccgctccggt agccgatagc gttttaactg atttcaaagt taacgctgca
    16321 aaggccctct cgccttatgc ctcggcgatt ctggtagatc aacaattctg ctaccgccag
    16381 gtggttgagc aaaacgcgat tgccaaaagt tgcgccatga ttgtcgccgc cgatgagttc
    16441 attcctggca acggtattcc ggtcgatagc gtggttattg accgcaaaat caatccgcta
    16501 cagatcaaac aagacggtgg caaagcctta aaactgctgg tgctgtggcg tagtgatgaa
    16561 gatgcgcagc aacgtctgga tatggtgaaa gagttcaacg gactgtgcca ctcacacggt
    16621 ctggtaagca tcattgagcc agtcgtccgc ccaccgcgtc gtggcgataa attcgatcgc
    16681 gaacaagcgg tcatcgatgc cgccaaagag ctgggcgaca gtggcgctga cctctacaaa
    16741 gttgaaatgc cgctttatgg caaaggtccg caacaagagc ttctctgtgc ttcacaacgt
    16801 ctgaatgacc atatcaatat gccatgggtg atcctctctt ccagtgtcga cgaaaaactg
    16861 ttcccgcgtg ccgtacgcgt ggcaatgacg gcgggcgcat cgggattcct ggcaggccgt
    16921 gcagtctggg catcggtcgt cggtttacca gacaacgagc tgatgctccg tgacgtttgc
    16981 gcaccgaaat tacaacaact tggcgatatc gtcgacgaaa tgatggctaa acgccgctaa
    17041 gatgaggata aaaggaatga aatggtttaa caccctaagc cacaaccgtt ggctggaaca
    17101 ggaaaccgac cgcatctttg attttggtaa aaattccgta gtgccgactg gttttggctg
    17161 gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc catctgtgga tcacagctcg
    17221 tatgttgcac gtttattccg ttgctgcggc gatgggtcga cctggcgctt acgcgttggt
    17281 tgatcacggt attaaagcca tgaacggcgc actgcgtgat aaaaaatatg gcggctggta
    17341 tgcctgcgtg aatgacgagg gcgtggtgga tgcctccaaa cagggctatc aacacttctt
    17401 tgctctgctg ggtgccgcca gcgccgtcac aacgggtcac ccggaagcgc gcaagctgct
    17461 cgattacacc attgagatta tcgagaaata tttctggagc gaagaagagc agatgtgcct
    17521 ggaatcctgg gacgaagcct tcagcaaaac cgaagagtac cgcggcggca atgccaatat
    17581 gcacgcggtg gaagctttct tgattgttta tgacgtcact cacgacaaaa aatggctgga
    17641 tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc gccagaaata atcattatcg
    17701 cgttaacgaa cacttcgata cccagtggga tccgctgccg gattacaaca aagataaccc
    17761 ggcgcatcgc ttccgcgcgt tcggaggtac accaggccac tggatcgaat ggggccgttt
    17821 aatgctgcac atccatgctg ccctggaagc ccgttgcgaa caaccaccag catggctgct
    17881 agaagatgcc aaaggtctgt ttaacgccac cgtgcgcgat gcctgggcac ccgatggcgc
    17941 ggacgggatt gtttataccg ttgactggga aggaaaaccg gtggtccgcg aacgtgtacg
    18001 ttggcctatc gtcgaagcaa tgggtacggc ctacgcgctc tacaccgtca ctggcgatcg
    18061 ccagtatgaa acctggtatc aaacatggtg ggagtactgc attaagtacc tgatggacta
    18121 tgaaaatggt tcctggtggc aggagctgga tgcggacaat aaggtcatca ccaaagtctg
    18181 ggacggcaaa caggatattt atcacctgct gcattgcctg gtgatcccgc gtatcccgtt
    18241 agcgcccggc atggctccag cggttgcagc gggtctgctg gatattaatg cgaaataact
    18301 gatctgaaaa tagggtgcgg gttttcccct caccctaacc ctctccccaa aggggcgagg
    18361 ggaccgtccg gtgcagtttt tgggtttcac atcagcctca cgggaggctg tatgtcgtta
    18421 attaaggtgc cttgtatgca aatcaccaat atgcattgca gtggacagac cgtttcactt
    18481 gccgcggggg attatcacgc gaccattgtg accgtcggtg ctggtctggc tgaattaacc
    18541 tttcagggtt gtcatttagt catcccgcac aaacccgaag agatgccgct ggcgcatctg
    18601 ggtaaagtgc ttattccgtg gccgaaccgg atcgccaacg gctgttaccg ttatcagggg
    18661 caggaatatc agttacccat taatgaacat agttcaaaag ccgctattca cgggctgctt
    18721 gcctggcgag actggcaaat tagtgaatta accaccacca gcgttaccct gacagctttt
    18781 ctgccgccca gttacggcta tccgtttatg ctggcttcac aagttgttta ttcgctcaac
    18841 gcacacacgg gtctttccgt tgagatcgca agccagaata tcggcactgt cgcagcgcct
    18901 tatggcgtgg gtattcatcc ttatttaacc tgcaacttaa catctgttga tgaatatctg
    18961 ttccagcttc cggcaaacca ggtttatgcc gtcgatgaac acgtaaatcc gacaacgctt
    19021 caccacgttg atgagctgga tttaaatttc acgcaggcga aaaaaatagc cgcgacaaaa
    19081 atcgaccaca cctttaaaac cgctaatgat ttgtgggaaa tgaccattac ccatccacag
    19141 caggcgcttt cggtaagcct ttgttccgat caaccctggg tgcaggttta tagcggtgaa
    19201 aaattacagc gtcagggact ggcagttgaa cctatgagtt gcccgcctaa cgcctttaat
    19261 tcaggtatcg acttattatt actggaacca ggaaaaccgc accgcctgtt ttttaatatt
    19321 tacggacaga gaaaataact tgtattgatt agcagtcaaa atcattgaag aacatttcac
    19381 tcgctaaagg caacattatg tatcttcaat ttattaagtt aaaaacagct cagcttctgt
    19441 aaagttttta tatatcagtt ttatgagaat cattttacat aagcctgtaa taacaggaag
    19501 gcaacaatct cgttctcaac tctatggaga ttagttatgg atacgccacg tccacagtta
    19561 ttagattttc aatttcatca gaataacgac agttttaccc tacattttca acaacgtctt
    19621 attttaaccc atagcaaaga taatccttgt ttatggattg gctcaggtat agcggatatc
    19681 gatatgttcc gcggtaattt cagcattaaa gataaactac aggagaaaat tgcgcttacc
    19741 gacgccatcg tcagccagtc accggatggt tggttaattc atttcagccg tggttctgac
    19801 attagcgcca cgctgaatat ctctgccgac gatcagggcc gtttattgct ggaactacaa
    19861 aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc agaggaccat
    19921 atctacggct gcggcgaaca gttttcctac ttcgatctgc gcggcaaacc gttcccgcta
    19981 tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg gcaggccgac
    20041 tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc tacgtttgtc
    20101 agcacgcaga agtattactg ccatgttgat aacagttgct acatgaactt cgactttagc
    20161 gccccggaat accatgaact ggcgctgtgg gaagacaaag ctacgctacg ttttgaatgt
    20221 gctgacacat acatctccct gctggaaaaa ttaactgccc tgctgggacg ccagccagaa
    20281 ctgcccgact ggatttatga cggggtaacg ctcggcattc atggcgggac ggaagtgtgc
    20341 cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg ttaacggcat ctgggcgcag
    20401 gactggtctg ggatccgtat aacctctttt ggcaagcgcg tgatgtggaa ctggaagtgg
    20461 aacagcgaaa actacccgca actggattca cgcattaagc agtggaatca ggagggcgtg
    20521 cagttcctgg cctatatcaa cccgtatgtt gccagcgata aagatctctg cgaagaagcg
    20581 gcacaacacg gctatctagc aaaagatgcc tctggcggtg actatctggt ggagtttggc
    20641 gagttttacg gcggcgttgt cgatctcact aatccagaag cctacgcctg gttcaaggaa
    20701 gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga cttcggcgag
    20761 tatctgccca ccgacacgta cctgcataac ggcgtcagtg ccgaaattat gcataacgcc
    20821 tagcctgcgc tgtgggcgaa gtgtaactac gaagcccttg aagaaacagg caagctcggc
    20881 gagatcctct tctttatgcg cgccggttct accggtagcc agaaatactc caccatgatg
    20941 tgggcgggcg accagaacgt ggactggagt ctcgacgatg gcctggcgtc cgtagtcccg
    21001 gcggcgctgt cgctggcaat gaccggacat ggtctgcatc acagcgacat tggcggttac
    21061 accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc
    21121 gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt
    21181 gacggcgatg cggaaactat cgcccatttt gcccgtatga ctaccgtttt caccaccctg
    21241 aaaccttatc tgaaagaggc cgtcgcgctg aatgcgaagt ccggtctgcc ggtcatgcgc
    21301 ccgctgttcc tgcattacga agacgatgca catacctatt ccctgaaata tcagtacctg
    21361 ttaggccgcg acattctggt cgctccggta cacgaagaag gtcgcagcga ctggacgctc
    21421 tatctgccgg aggataactg ggtacatgcc tggacgggtg aagcgttccg gggcggggaa
    21481 gttaccgtta atgcgcccat cggcaagccg ccggtctttt atcgcgccga tagcgaatgg
    21541 gcggcactgt tcgcgtcgtt aaacagcatc taatcacatc cgcccgtggg gaaactcatg
    21601 ggcaaaggga gaacatcgat gagtcacatc acaacggaag atccagcaac tttacgcctg
    21661 ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat cctgctggat
    21721 atcggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc aggcacctat
    21781 ggcggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat gggtaccggc
    21841 attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc ttttattctg
    21901 tatgcgtcat tcccggtcac cttactggcg atcgccaact ttgtcggcac accgtttgat
    21961 gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact gtttttcagc
    22021 atgatgaact gctcctacgg cgcaatggtc ccggcaatca ccaaaaaccc caacgaacgc
    22081 gcctcactgg cggcatggcg tcagggcggc gctacgctgg gcctgctgct gtgcacggtg
    22141 ggattcgtgc cagttatgaa tcttatcgaa ggtaatcaga aacttggcta tatcttcgcc
    22201 gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctactc aggcgtgaaa
    22261 gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa agcctggcct gttgcaatct
    22321 ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt gtattgccaa cctctgcacc
    22381 ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta cgtacttaac
    22441 gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt catcggcgtg
    22501 ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga aggtctatat cggcggcctg
    22561 ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc ggtcagcttt
    22621 gtggcgttct cctgcctggc gttcttcggc tcagcgtttg ttaacagcct gaactgggcg
    22681 ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc ggaaggcacg
    22741 gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc
    22801 ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga ccacaccatt
    22861 gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt caccattgtg
    22921 gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt ggaagagata
    22981 gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc gttataagga
    23041 gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga aaaaatcgcc
    23101 tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct gtatctcctc
    23161 aaactttata ccgatgaatt agggatgcct gcttactatg gcgggattat ctttctggtc
    23221 gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga ctcgcggaaa
    23281 aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcagt tccggcggcg
    23341 ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa aacgacgatt
    23401 gccaccgcgc tgtttatgat gtttggactt tcatacagtc tgatgaactg ctcgtatggt
    23461 gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc ggcttaccgc
    23521 cagggtggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc gttgcagtcg
    23581 cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc cattggcggc
    23641 tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga cacagcgcca
    23701 accggacata aagccagtat tctcaaatct ttttgcgcga tatttcgtaa tccaccattg
    23761 ctggttttat gcattgctaa cctgtgtact ctggcggcat ttaatatcaa actggcgatt
    23821 caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg gatgggattc
    23881 ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc cagtaactgt aaaatgtttt
    23941 ggtaaaaaac aggtttattt agctggcatg gtgctatggg cggtgggtga tatactgaat
    24001 tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc cttttttggc
    24061 acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga ttacggtgaa
    24121 tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt ctttcgtaaa
    24181 atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat tggttatgtt
    24241 cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat ttttatctgg
    24301 ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac actcaatgaa
    24361 aaacggtttg cattgattat tgaggaaatc aaccaacgaa aaaataaaga gatggcgaca
    24421 gaaaaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc catcctggtg
    24481 ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta atgcaataat
    24541 tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg aaaacaggga
    24601 ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt ataacttcga
    24661 tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag atgaactaaa
    24721 acatggctat aatgaaattg aaggttggta tcctttattc aaaccaaccg ataaattaac
    24781 catccagccc ggtggcttaa t
//
LOCUS       DAJTRQ010000048        24436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000048 DAJTRQ010000000
VERSION     DAJTRQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..176)
                     /locus_tag="OO332_002053"
     CDS             complement(<1..176)
                     /locus_tag="OO332_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5942539.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLE"
     gene            complement(269..841)
                     /locus_tag="OO332_002054"
     CDS             complement(269..841)
                     /locus_tag="OO332_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5942540.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            1059..1919
                     /gene="tesB"
                     /locus_tag="OO332_002055"
     CDS             1059..1919
                     /gene="tesB"
                     /locus_tag="OO332_002055"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5942541.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1968..3254)
                     /gene="amtB"
                     /locus_tag="OO332_002056"
     CDS             complement(1968..3254)
                     /gene="amtB"
                     /locus_tag="OO332_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5942542.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3284..3622)
                     /gene="glnK"
                     /locus_tag="OO332_002057"
     CDS             complement(3284..3622)
                     /gene="glnK"
                     /locus_tag="OO332_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5942543.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3803..5584)
                     /locus_tag="OO332_002058"
     CDS             complement(3803..5584)
                     /locus_tag="OO332_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5942544.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5577..7349)
                     /locus_tag="OO332_002059"
     CDS             complement(5577..7349)
                     /locus_tag="OO332_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5942545.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7379..7837)
                     /gene="decR"
                     /locus_tag="OO332_002060"
                     /pseudo
     CDS             complement(7379..7837)
                     /gene="decR"
                     /locus_tag="OO332_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7991..8809)
                     /gene="cof"
                     /locus_tag="OO332_002061"
     CDS             complement(7991..8809)
                     /gene="cof"
                     /locus_tag="OO332_002061"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5942546.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8909..10608
                     /locus_tag="OO332_002062"
                     /pseudo
     CDS             8909..10608
                     /locus_tag="OO332_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10673..11368
                     /gene="queC"
                     /locus_tag="OO332_002063"
     CDS             10673..11368
                     /gene="queC"
                     /locus_tag="OO332_002063"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5942547.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11420..11818)
                     /gene="fadM"
                     /locus_tag="OO332_002064"
     CDS             complement(11420..11818)
                     /gene="fadM"
                     /locus_tag="OO332_002064"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5942548.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11912..12283)
                     /locus_tag="OO332_002065"
     CDS             complement(11912..12283)
                     /locus_tag="OO332_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5942549.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12434..14305)
                     /gene="ppiD"
                     /locus_tag="OO332_002066"
     CDS             complement(12434..14305)
                     /gene="ppiD"
                     /locus_tag="OO332_002066"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5942550.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14497..14769)
                     /gene="hupB"
                     /locus_tag="OO332_002067"
     CDS             complement(14497..14769)
                     /gene="hupB"
                     /locus_tag="OO332_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5942551.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14978..17332)
                     /gene="lon"
                     /locus_tag="OO332_002068"
     CDS             complement(14978..17332)
                     /gene="lon"
                     /locus_tag="OO332_002068"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5942552.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17520..18794)
                     /gene="clpX"
                     /locus_tag="OO332_002069"
     CDS             complement(17520..18794)
                     /gene="clpX"
                     /locus_tag="OO332_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5942553.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18920..19543)
                     /gene="clpP"
                     /locus_tag="OO332_002070"
     CDS             complement(18920..19543)
                     /gene="clpP"
                     /locus_tag="OO332_002070"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5942554.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19790..21088)
                     /gene="tig"
                     /locus_tag="OO332_002071"
     CDS             complement(19790..21088)
                     /gene="tig"
                     /locus_tag="OO332_002071"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5942555.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21432..21749)
                     /gene="bolA"
                     /locus_tag="OO332_002072"
     CDS             complement(21432..21749)
                     /gene="bolA"
                     /locus_tag="OO332_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5942556.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            22054..22632
                     /locus_tag="OO332_002073"
     CDS             22054..22632
                     /locus_tag="OO332_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5942557.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22676..24151
                     /gene="ampG"
                     /locus_tag="OO332_002074"
     CDS             22676..24151
                     /gene="ampG"
                     /locus_tag="OO332_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5942558.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5737 a         6717 c         5906 g         6076 t
ORIGIN      
        1 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
       61 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      121 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      181 gcgtttccgg attcttacac agccactgga tcggtcaact gatccttaac tgatcggcat
      241 tactccgggc ggagagggtt aacgtcgctt aatattgcgt aggagcggga actgccgaag
      301 acggattcac ctgagttggt gaagtcgaag gtacggtagt cgttgcgcca ccgctggcct
      361 gaaccggcac ggcggtttgc tgtaccggca ccaatgtcag gtcggcttta gttccgccct
      421 ggttgatcac cggctgaacg gtatcggtga taaataccag tttgtcattc acggtaatcg
      481 ccgcactcaa cagaatacgc gcgttcggct gaacatctgc cgggttaaat ggcagaacaa
      541 agctgaatgg tgactgttta ccttcagtac gcaccgcttt ctgcgccagc acttttgacg
      601 gtgcatcggc taacgacgcg tcagaaagtg tcacggtcag cacagcatca ggcggcagtg
      661 cgactttctg acggatccag acggtaccgg agacattcgg ttgctggata gcaggttgtt
      721 gtgttgctga aatagacgta tttgcagccg gggctggcgt ctgaatatcc gcgcttttat
      781 ctgcgcaagc cgccaacgca atcgcaaccg ctaaaccact ggccatgtgc acgagtttca
      841 ttcatttctc cttattatca atgcaccagc gggctaactt tcctcgccgg aagagtggtt
      901 aacaaaatag taacgtcaac aagtgtggca cacatcacgc atttctgcct gtaattagcc
      961 cgtaattcag actattgcac ccatcggacc agtggcaaat ttgcgttata ctcaactcac
     1021 tttggcttgc tgcggcagct ttgttactgg agagttatat gagtcaggcg ctaaaaaatt
     1081 tactgacatt gttaaatctg gaaaaaattg aggaaggact ctttcgcggc cagagtgaag
     1141 atttaggttt acgccaggtg tttggcggcc aggtcgtggg tcaggccttg tatgctgcaa
     1201 aagagaccgt cccggaagag cggctggtac attcgtttca cagctacttt cttcgcccag
     1261 gcgatagtaa gaaaccgatt atttatgatg tcgaaacgct gcgtgacggt aacagcttca
     1321 gcgcccgccg ggttgctgct attcaaaacg gcaaaccgat tttttatatg acggcctctt
     1381 tccaggcacc agaagcgggt ttcgaacatc aaaaaacaat gccgtccgcg ccagcgcctg
     1441 atggcctgcc ttcggaaacg caaatcgccc aatcgctggc gcacctgctg ccgccagtgc
     1501 ttaaagataa attcatctgc gatcgtccgc tggaagtccg tccggtggag tttcataacc
     1561 cactgaaagg tcacgtcgca gaaccacatc gtcaggtgtg gatccgcgca aatggtagcg
     1621 tgccagatga cctgcgcgtt catcagtatc tgctcggtta cgcttctgat cttaacttcc
     1681 tgccggtagc tctacagccg cactgcatcg gttttctcga accggggatt cagattgcca
     1741 ccattgacca ttccatgtgg ttccatcgcc cgtttaattt gaatgaatgg ctgctgtata
     1801 gcgtggagag cacctcggcg tccagcgcac gcggctttgt gcgcggtgag ttttataccc
     1861 aagacggcgt gctggttgcc tcgaccgttc aggaaggggt aatgcgtaat cacaattaaa
     1921 aaaatagccg gaggtgaaaa ccgtccggct attttttgca gtgcttgtta cgcgttatag
     1981 gcattctcgc cgtggctgtt gacgtccagc ccttctcgct cttgttcttc cggcacacgt
     2041 aggccaaccg tcagatccgc cagtttgtag ccaataaatg ccaccacacc ggaccagacg
     2101 atcgtaatgg cgatgctttc caactgtacc agcaactgat ggcccatcgt cacaccttca
     2161 gcgaagccca cgccgcccag agaactggcg gcaaaaatcc cggtcatgat acagccgaca
     2221 atgccacaaa cgccgtgcac accgaagaca tcgcagggat catccacccg cagcaagcgt
     2281 ttgagcatgg taacgcccca caaacccgcc agaccagcta ccacgccgat aatcaacgcg
     2341 ccgccaaccc caatgtagcc gcacgctggc gtcacgccga ccagaccggc aatcgcgcca
     2401 gaacacgctc ccagcagaga aggcttacca cgcagcgccc attcaccgaa gatccagcca
     2461 agaattgccg ccgccgttgc gaccacagta ttcacaaatg ccagtgccgc gatttcattc
     2521 gccgtgcccg ctgacccggc gttaaagcca aaccaaccga tatagagaat ggcagtcccg
     2581 gtgaagacca tcggcaggtt gtgcggttta aacgcctctt taccgaagcc cacgcgtttg
     2641 cctatcagat acgcgcccac cagaccggcg attgctgcgt taatgtgcac caccgtgcca
     2701 cccgcgaaat ccagcgcacc gtgagaagcc agcaaaccac cgccccacac catatgcgca
     2761 atcggaatgt aagagagcgt cagccatacc accacgaaaa tcaacacagc tgagaagcgg
     2821 attcgttccg ccagcgcccc aactatcaag cctacggtaa tgcaggcaaa cgatccctga
     2881 aacgctacgt ggatatactg ataaatgctg cccatcaccg ccgtcagttc gatgtttttt
     2941 agcatcaacc agttaatgtt gccgaagaag ttgttgccct caccaaacgc cagcgaataa
     3001 ccgtaaacca cccagagaat acagaccagt gcaaacgtca ccgtcacctg cgtcagcatc
     3061 gacagcacgt ttttgccgcg aatcaaccca ccgtaaaaca gggcaatccc cggaatagtc
     3121 ataaacagca ccagcgcagt acaaatcatc ataaacgcat tgtcggcttt atcggccacc
     3181 gcaggtgcag ccattaccag tcccggcagc atcgccagtg aagcaagccc agtttttatc
     3241 gtcgctatct tcatttttcg ttcctgttgc tgtgtgccag agattacagc gccgcttcgt
     3301 cggcttcgcc ggtacgaata cgaatgacgc gttgcaattc agcgacgaag attttgccgt
     3361 cgccaatttt tccggtgtaa gccgccttac tgacgatatc gatcacttca tcgagttggt
     3421 catcagcaat cgccacatca atttttactt ttggcaggaa gttgacgctg tattccgccc
     3481 cccggtacag ctcggcatgc cctttctgac gcccgaaacc tttcacttcg gtgacggtca
     3541 ggccctgaat accaatggaa gataacgctt cacgaacgtc ttccagcttg aatggtttga
     3601 ttatcacggt caccagcttc atagatcccc tccggtcaga attcggtaat ggttctgcta
     3661 cacgaagaag gtattgcaag cggtgtgcca gaaatgaaaa acgagaaaga gagcaggaag
     3721 ttaagaagat tgaaaatgaa aacgcactat gacagtgcac agtgcgttac atttttgcac
     3781 caacgatgaa atattgcgct attcaggcgc tcaatgattc ctcttcacgc acgctggctg
     3841 ccagctcttc gcccgcaagt tgcagttgat acatctgcca gtagcgtccc tgggccgcca
     3901 gcagttgctg gtgagtgccc tgctccacgg cttgcccacg atgaagcacc agaatggtgt
     3961 cggcatcaat aattgttgat aagcggtgag caatcaccac cagcgtggta tgttcacgca
     4021 ccgccgccag agcatgttga atcgcctgtt cagtaccgga gtcaatgctg gcggttgcct
     4081 catcaaggat caggatttgc ggcgtctcca ccagcacgcg cgccagtgcc agcagttgct
     4141 tttgcccgac tgagagatta tttccctgct cgcccagcgg cgtataaata caatcactca
     4201 tgctgcgcgc cagctcagcc agttgcacgg tttccagcgc ctgccagacg cgttcttcgg
     4261 agatatcccg ccccagcgtc acgttggcga ggaaggtatc cgccagcacc accggatctt
     4321 gctgcaccat tgccacgccc tggcgcagcg cgctatgact tagcgaactt aatggacgac
     4381 catcaaggtg aatctcacct tccgttagcg ggtaatagcc catcaataaa ctggcaaggg
     4441 tacttttacc actgccggta tgcccgacca gcgccacaaa gttacgcgaa ggcacagaga
     4501 gattaatgtt ctttagcacc agattgtcat cgcgataagc aaatgacacg ttatcgactt
     4561 cgatggtgcc actctgtaac gggtaatcat cattgccata ttgctggcgc ggtccgtcca
     4621 tcagttcaaa cacgcgctca ccagcaacaa cagcctgttg cagcatcgcc tgttgcgtgg
     4681 tcagttcgat taagggttcg ttaagtcgcc caagatagct gataaacgca tacaacacgc
     4741 ccacttcaat ggtgccgctg gcggagaagc caaacagcat caataagcca caaagaatga
     4801 gcgatgaaaa cagactcagc agcggacgca gcagaaaacc gtcgaggcgc agggtttgca
     4861 tcctagccat atagtgtgaa cgactggcct cccccatacg ttcgccaaat cgcgcctgct
     4921 gacgaaactg ctggataacg ctcatgccat tgatgatttc gttaaagccg tcgttgatat
     4981 ccgccagata ggcgcgcaca cggcggacaa tcggcgtgtt gtaacgctgg tatatcacca
     5041 ttaccaccag caccaccggg aaaatcatta tcgccaccag cgccattcgc cagtcgaggc
     5101 tgaacatcgc caccagcatc gcgcccacca gtgcggcact gcgcaggaca gttgccacta
     5161 cggtaacgta gagatcgcgg atcacttcag tgtcattagt gacgcgggag atcacctgcc
     5221 cgacgggttg ggtatcaaac tcgcttaatg gctggcgtaa cgcagcatcc atcacgtcgg
     5281 tacgcaactg ttgcactacg cctactgccg cccgattaaa cagcagcgac tgagcgtaat
     5341 gtagcccggc ggcaaacagt tgtagcccaa catacgccgc agccagccct gcaaccactt
     5401 tcaacggcag gttatttttc gctaccatat tgtcgataaa atagctgata agcagcggcc
     5461 cactgacttc tgccgccgcc gcaacccaca tcatcaggac cgcaatcccc agcggtttac
     5521 gccacggcga accatacgct aacaggcgct tgagagtcgg ccacagttgg ctaaaactac
     5581 gcatcgacgg cctcctcgcg aatttccgga acgtcgtcga gcgccgcctc cagttgttga
     5641 tagcgataca tatcgcgata ccagccgctt tgttgcgcca gctcatcatg attgccacgc
     5701 tgggcgatat gtccgtgctg catcacaata atttcactgg cttccgtcag tgccgaaagg
     5761 cgatgggcac tgatgattac cgttcttccc tgtccccact gacgcaggtt atgcaggatc
     5821 tggtgctctg tgcgtccgtc caccgccgaa agcgcatcat caaggatgag gatttccgcg
     5881 ttgactaata acgctcgagc aatggagata cgctgttttt gcccaccgga aagcatcacg
     5941 ccgcgctcgc ccacttctgt atcgtaacct tgcggtagac gcaaaatatc gtcatgtacg
     6001 ctggctaacc gcgcgacatg ctcaatctct tgctgggtgg cattcgggca acccagcgcg
     6061 atgttattcg ccacggtgtc agaaaaaagg aatggcgtct ggctgaccac cgccaggcgg
     6121 ctacgccagc tatcgagttg taacttcgtc agaggaatat catgaaagcg aatatccccc
     6181 tcgctgacgt cgaaatgacg ctgaatgagc gacaacaggg tacttttgcc ggaaccagtc
     6241 ggcccgcaaa tacccagcat ctgaccgggt ttcagggcga aattgacgtt ttccagcgca
     6301 ggatggtcag tctgcggata cgtgaactgg tgaatattta catccagttc gccacgccct
     6361 tccggcacga gttcgctacc atcgttcacc accggcgctt ccgccagcat cgcgcgaata
     6421 cggctgtatg cagcactacc acgttccaca atgttaaaca tccatgccag cgccagcatt
     6481 ggccaaatca tcagacctaa atacatcata aaactggtga gctggcccag cgttaaactg
     6541 ccctgcacca ccatccaact accgccgcca atcgccagca agttcgccat accaatcgcg
     6601 atatagatgg tcgggtcgaa gcgagcatca atacgcgcca cccgcatgtt ttttttgccg
     6661 gtatcttcgg catccgcggc aaataacgcc gactggcgat cttccagacc aaaggctttg
     6721 atcatgcgga tactggtgag gctttcctgg gtgcggtcat taagactgga aaacgccgcc
     6781 tgtgccagct taaagcgttc atgcaaggca tcgccgttgc gcttaatcat gatcgccatc
     6841 actggcatcg gtaacagggc aaataaggtc aactgccagc taatttgcgt agacatcata
     6901 atcaacacag cgcagcccat taccagtgaa tccaccagcg tcagcacccc ttctccggcg
     6961 gcaaacacga cgcgatcgac gtcattggtc gcacgagcca tgagatcacc cgtgcgatga
     7021 cgcaggtaaa actccgggtg ctgccggctt agctgacggt aataatcttc acgcagttca
     7081 acagccagtt ggtaagacgc gccaaacagc aatacgcgcc agacataacg caggagataa
     7141 accacaacgg caatcagcac catggtggcg atccacatca ggatcagccc ggtagtaaag
     7201 tgttgttctg tcacgccatc gacaacaata ccaaccactt ttggcggaac cagttgcagc
     7261 atcgcgataa tgacaagcaa ggcgacagcc ccaagatagc gacgccattc ccgacggaaa
     7321 taccagctta attgagcaaa taatcgcacg cgttgtgtcc tgacctgatt ctggatattt
     7381 attcgatggg taaagaagtg gtgtatttaa tctgttccat cgcgaagctg gaagtgacgt
     7441 ccgacagccc cggcacgctg tttaccagac gcttataaaa ctcgtcgtag cgtttcatgt
     7501 cggcaaccta gacgcgcatc agataatcgt attcaccagc catgcgccag aaccccagca
     7561 cttctggcat ttcggtaacc accgtgacaa agcggcaata ccattcgctg ctgtgatgtt
     7621 gcgtttttat cagcacaaag gcggtcaggc cgaggcctat tttttccgga tccagcaggg
     7681 cgactttgcc gataaggata ccgtcgtcct ccagccgttt caggcgcttc cagcaggggg
     7741 tggttgtcag attaacggct tcagccagtg cctgcaaaga gagggtgcaa tcctgctgca
     7801 gtaaggccag cagcttacgg tcaattttat ctaacatagc ccttccacgg agaatttttt
     7861 tctcttttaa ttctatttta aaggtgaaat cgcgacaatt tattctgtgc ggtttaaaca
     7921 ccacggcaca aaatgacaaa ttcaacgcaa aaaaaaaccg ggcaattgcc cggtgctgga
     7981 agggatctcg ttattcgggg gaataaggta gatgtggata gtccagccag tgcgtcaaat
     8041 agtgagagac agcctgattt cggcaatgtc caatcaccgg taaatgcggg agctccgcgc
     8101 gcagttgcgg catcgcattg cccataataa acccgctacc gacgctgcct aacatttcgc
     8161 gatcgttcat cgcatcacca aaggccatgc aatcgcgcaa cgataaacct aaatgttggg
     8221 tcagcaccgt caatgcagcg cctttattgc agcccaccgg cagcacttcg aggcaatccg
     8281 tggcggaaaa acacaaatgt gcacgctcgc ctaatgcttc gtatagctgg atctgcaagc
     8341 gtgtaagatc gtcgtgatcg ccacagaagc agatcttggt gacgctgccg agtggcattt
     8401 ttttgacatc gattatctga taacgaaaac cgctatagac aaatacctgc aacaacgcag
     8461 ggctctcttt cccggtaaac caaccgtcgt cattgaagat atgcatgctg gctcgggtat
     8521 cccattgctg atacagcacc agctccgcga catccgcagg taaatcatca cgatgtaaaa
     8581 gttcaccttc cagagaatgc acgcgcgttc cgttgccggt aatcaaatat gcatccagcg
     8641 atagcgcccc gagaatatgc tgcatctcca gcgcatgacg ccccgtggca aaagtgaggg
     8701 taatgtcgcg ttcacgcagt cgcgccaaag tagagagggt tttctcacct aaatgatggt
     8761 caggcatcaa taaagtgcca tccatatcaa atgctgccag acgagccatt tctttctcca
     8821 ctctgtgaca cggttaatgg tgattgagta tcacctgata tatacggaag taatagtgaa
     8881 tagttaaata atattgttcc gggtttatat gcgattgctc aaccgtctta accagtatca
     8941 acgtctgtgg caaccttcag ccggaaagcc gcaaaccgtc accgtcagcg aactggccga
     9001 acgctgtttt tgcagcgaac gccatgttcg tacgctgttg cgtcaggcac aggaggcggg
     9061 atggctggag tggcaggcgc agtcaggacg cggaaagcgc ggacaattac gctttctggt
     9121 cacgccggaa tcgctacgca atgcgatgat ggaacaggca ctggaaaccg gaaagcagca
     9181 agatgtgctg gagctggcgc aactggcccc aggtgagctg cgcactctgt tacagccgtt
     9241 tatgggcgga caatggcaaa cgatacaccc acgttgcgta ttccctacta tcgcccgctc
     9301 gaaccgctac aaccaggctt tttgcccggc cgtgccgagc agcatctcgc cgggcagata
     9361 ttttccggcc tgacccgctt cgataataat actcagcgcc cgattggcga tttagcgcat
     9421 cactgggaaa cctctactga cgggttacgc tgggactttt atcttcgttc aaccctacac
     9481 tggcataacg gcgatgcagt aaaagcctca cacttacacc agcgattatt gatgctgtta
     9541 caactgccag cactggatca attatttatt agcgtgaagc gtattgaagt cacccatccg
     9601 cagtgtctga ccttcttttt acatcgcccc gattactggc ttgcgcaccg gctggcgagc
     9661 tattgcagcc atctggcgca tccgcaattc ccactgatcg gcactggtcc ttttcgctta
     9721 acacaattca cagcggagct ggtgcgcctg gagagccatg attattacca tttgcgtcat
     9781 ccgctgctta aagcggttga gtactggata actccgccgc ttttcgaaaa agatttggga
     9841 accagttgtc ggcatcccgt gcaaatcacc atcggcaaac cggaggagct gcaacgggtc
     9901 agccaggtca gtagtggcat cagtttaggt ttttgctatt taacgttgcg caaaagtccc
     9961 cgactctccc tctggcaggc gcgaaaagtg atctccatta ttcatcaatc cggtttatta
    10021 caaacgttag aagtcggaga aaacctgatc accgccagtc atgcattact gccaggctgg
    10081 actattccgc actggcaagt accggatgaa gtcaaactac cgaaaacctt gacgctggtt
    10141 tatcacctac cgatagaact tcataccatg gcagaacgcc tacaggcgac actggcagcg
    10201 gaaggctgtg aactcacaat tatttttcat aacgcaaaaa actgggacga cacgacccta
    10261 ctggcacacg cagacctcat gatgggcgac agattaattg gcgaagcacc ggaatatact
    10321 ctggagcaat ggctacgttg cgatccacta tggccacatg ttttcgacgc tccagcatat
    10381 gcacatctgc aatcgacgct ggacgcggtg caagtaatgc ctgatgagga aaaccgattt
    10441 aatgccctga aagcggtttt tagccagtta atggcagacg cgacgctgac gccgctgttc
    10501 aactatcact atcgcattag tgcccctccc ggcgtgaacg gtgtgcgact gacaccgcgc
    10561 ggctggtttg aatttaccga agcctggctt cccgcgccgt cgcaatgaat agctggtccg
    10621 ggttaatcgc aggcgttacc ataacgcttt tattgttcaa gcaggattat ctatgaaacg
    10681 tgctgtcgtt gtgttcagtg gaggccagga ttccaccacc tgtctggtgc aggcattaca
    10741 acaatatgat gaagtccatt gcgtgacgtt cgattacggt cagcgacatc gcgcagaaat
    10801 cgacgtggca cgcgaactgg cgctgaaact gggagcacgc gcgcataagg ttcttgatgt
    10861 aacgctgctc aacgagctgg cggtcagcag cctgacacgt gacagcattc cggtgcctga
    10921 ttatgaacct gaagccgatg gcatcccgaa tacgtttgtc ccagggcgta atattttgtt
    10981 cctgacgctg gcggcaatat atgcgtatca ggtaaaagca gaagccgtaa ttaccggcgt
    11041 ctgtgaaacg gatttctccg gctacccgga ttgccgcgat gagtttgtga aagcactaaa
    11101 ccatgccgtc agtttgggca tggcgaaaga tattcgtttt gaaacgccgc tgatgtggat
    11161 tgataaagcg gaaacctggg cgctggcaga ttattacggc aaactggatt tagtccgtaa
    11221 cgaaacgttg acctgctata acggcattaa aggcgacggt tgcggtcatt gtgcggcatg
    11281 taatttacgc gccaacggtt tgaatcatta tctggccgat aaaccgacgg tgatggcagc
    11341 gatgaagcag aaaaccgggt tgaggtaatt attccgggtg tcgccggatg cggcttgagc
    11401 atccggcacc acaaaacgtt tacttaacca tctgctccag cttttcgcgc aattcccctt
    11461 ccagagctaa tgctttctgc gttttaagat caatacaaac aaacgtaata agcgcatccg
    11521 ctaccacctg cccttccggc tccagtgtaa tgacctggct taagatgccg cttttaccgt
    11581 ttaattgctg caactgactg gtaatagtta acaggtcgct taataccgct gggcgacgat
    11641 agttaatatt gatattgacc acgacgaagg cgatgttatg ggccgtcatc cactgaaaac
    11701 tgtcgctatt ttccaaccca tcccagcggg cttcttcgag aaattcaagg tagcgggcgt
    11761 tgttgacgtg ctggtaaacg tcgagatgat atccacgaac tttgatttgt gtttgcatag
    11821 cgcaataacc ttacgttgtt gttataagta cagaggtcat aactggtatg acctcttcag
    11881 tctggcaaaa aattgccact atgcaaatta attacagggt taataccgcc agattacgtt
    11941 ccaccagcaa attgcccatc cccggcacct gctttagatc ctccaccgtt ttaaacggac
    12001 cgtactcttc gcgataactg acaatcgcct gcgctttctt caggccaacg ccattcatcg
    12061 cgcgggctag ctcttccgcg ctggcattat taatgctgac ccgggtgcct tcttcgtcac
    12121 tggctttcgc cggtactgct gctttacttt gtgctgcagg agcttccgct ttggtttcta
    12181 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
    12241 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
    12301 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
    12361 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
    12421 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
    12481 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
    12541 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
    12601 gaaccacatt accttgcata tcagtcgcca taccgtagct cggtttgtct ttcgctggca
    12661 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
    12721 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc acctttgccg gctttcaaat
    12781 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
    12841 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
    12901 tgacgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
    12961 acgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
    13021 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
    13081 cagcttgctc tgcaccggcc agagactcgg tttcgttgct tgccgcatcg ctcactttct
    13141 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
    13201 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
    13261 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
    13321 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
    13381 cagagataat atcggcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
    13441 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
    13501 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
    13561 cgctaaccgg ttgctgcatc gttgcggcaa ccagcttgat gtaactcacg cggaattgtt
    13621 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
    13681 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
    13741 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
    13801 cgccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
    13861 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
    13921 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
    13981 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
    14041 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
    14101 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
    14161 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
    14221 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
    14281 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
    14341 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
    14401 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
    14461 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
    14521 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
    14581 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
    14641 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
    14701 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
    14761 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
    14821 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
    14881 cccgatccgc tatctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
    14941 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
    15001 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
    15061 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
    15121 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
    15181 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
    15241 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
    15301 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
    15361 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
    15421 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
    15481 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
    15541 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
    15601 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
    15661 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
    15721 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
    15781 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
    15841 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
    15901 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
    15961 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
    16021 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
    16081 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
    16141 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
    16201 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
    16261 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
    16321 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
    16381 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
    16441 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
    16501 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
    16561 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
    16621 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
    16681 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
    16741 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
    16801 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
    16861 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
    16921 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
    16981 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
    17041 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
    17101 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
    17161 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
    17221 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
    17281 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
    17341 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
    17401 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
    17461 aattaaaggg gagataaaat cccccctttt tgtttaacta attgtatggg aatggttaat
    17521 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
    17581 ccatcaatta ccgactcgtc aataaccact ttttcgacgt cttccatgga cggcagatcg
    17641 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
    17701 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
    17761 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
    17821 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
    17881 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
    17941 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
    18001 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
    18061 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
    18121 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtactcct
    18181 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
    18241 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
    18301 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
    18361 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
    18421 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
    18481 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
    18541 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
    18601 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
    18661 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
    18721 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
    18781 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
    18841 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctagata agtatagcgg
    18901 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
    18961 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
    19021 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
    19081 cacgagcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
    19141 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcaacaaga
    19201 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
    19261 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
    19321 gatagatatc tttttctggg ttttccgctt ccaggaacag catctgcgcc acaatcaggt
    19381 tagccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
    19441 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
    19501 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
    19561 aattgaaaaa aacctgctgt accgattgta accttatgga cggattatca gctagtccct
    19621 gtgttatggg tacggcatca ccatatttca agcataacaa tcttttgttg ttacgctaac
    19681 accgaaagag gcttttcgca cggctccatg caaaattcat gacaaaaaaa agcccaccac
    19741 ctggcggtga cgggcctttg tgcgaattta gcgcgttatg ctgcgtaaat tacgcctgct
    19801 ggttcatcag ctcgttgaaa gtggtttctt tttcagtcac tttcgctttc gccagtacag
    19861 cttcaacagc ctgttcttcc agagcaacat tgcgcatgtt gtccatcagt tctttgtttt
    19921 tgctgtagaa ctcgataact tctttcggat cttcgtacgc agaagccatc tcttcgatca
    19981 ggcctttcac gcgctcttcg tcagctttca gctcgttggt gcggataact tcgcccagca
    20041 gcaggccaac aactacgcgg cgtttagcct gttcttcgaa cagttcgcgc ggcagttcca
    20101 gagcttgttt ttcgttgcca ccgaaacgct gtgcagcctg gcgacgcaga acgtcgattt
    20161 cgctgtcgat cagcgcagcc ggtacgtcga tgtcgttagc ttttaccaga ccttcgatcg
    20221 cctgagactt aacgcggtta cggatggcgc tcttcagctc gcgctccatg tttttacgca
    20281 cttcagcgcg cagaccttct acggaaccat cttcaacgcc gaaacgtttg atgaattctg
    20341 cagtcagttc cggcagttca cgctcttcaa ctttcttcag gttgatagcg aatttcgctg
    20401 ctttaccttt caggttttct gcgtggtatt cttccgggaa ggtcacgtcg atggtgaact
    20461 cttcgccagc tttgtggcct ttgataccgt cttcaaagcc cgggatcata cgaccctggc
    20521 ccatcgccag tacgaaatca gacgctttac cgccttcgaa ctcttcgccg tctacagaac
    20581 cggtgaagtc gatggttacg cggtcttctg cttcaacagc gccgtctttt tctttccagg
    20641 tcgcctgctg tttacgcaga gtatccagca tgccgtcaac gtcagcgtcg gtcacttcaa
    20701 cgatcggttt ttcaacttcg atcgcttcca gaccctgcag ttcaacttcc ggataaactt
    20761 caaactctac agagtaagtg aagtcttcac ccagcttgta ttcgcccgga acataagtcg
    20821 gtgcgccagc cggattgatt ttttctttaa tgatggcgtc aatgaagtta cggctcatca
    20881 ggtcacccag aacgtcctgg cgtacagacg cgccataacg ctgagcaacg atattcattg
    20941 gcactttgcc tttgcggaag ccgtcaatac gtactttttt cgcaacgttg accagctcgc
    21001 ttttaacagc ggtctcgatg ctgtcagcag cgatagtaat cgttacacgg cggccaaggc
    21061 cttgagtggt ttcaactgaa acttgcatct tgttacctca aaaaatcaca gtgctcggtc
    21121 aactcttacc ccgcaagcac aggttgttgg cttcgcggaa ccgggatgtt cttataatca
    21181 atcacattcc ctgttaccag aatcatcccg aaagcattca aataggacgc ggcattatag
    21241 cggcatcact tttatgagtc gagaacggtt attgcgcgct gtcgcgtatt ttttcacaat
    21301 tcccgggcaa ttttcgtctg aatgctgggc aaaaagcgca aaatccagac aaaacaaaac
    21361 ggcccgcagg ccgtttttca taaaatcttt agcaacatac tggaaaagcg ccgacagttg
    21421 caaatgcgtt tttacgcgat gcttcctgct ccacgacagg gaggagaggc aaagacggtg
    21481 tcctgcaacc cttcccactc cttaatagtg taagtatgca gagccagcgc atgaacggta
    21541 gtagagagtt cctccgctaa agtactgtaa atcattcgat gacgattcag aaaacgttca
    21601 cccgtaaaac gatcgctgac cagcacaact ttaaaatggc tttcagagcc ggctgggaca
    21661 ttgtgacgat agctttcatc cactacttcg aggaatacgg gttggaacgc cgcccttaat
    21721 ttttcttcta tccgctcacg tatcatcatg aaatatctcc tccgacaacg ctgagatgtc
    21781 atccatccct ttaaatacta gccgctttta ccgtttccat tgcagcttaa caacaaatat
    21841 ttaggtttcg tcctggtttt tccgtcaagc gtatgaagtt tactgattta aagattgaaa
    21901 cacaggcctc gcatcagcgt ttcttttacc aggggtgatt atttcgtcaa catgatgaat
    21961 ttacatgcct tacccacttc ccctcgccgt tggcaatgtt atgatggcgg gaatttttac
    22021 caaacacgct taagagtctc tgagagcccg aacatgttca aaaaaatcct cttcccgtta
    22081 gttgctctgt ttatgcttgc aggatgcgca aaaccgccaa caactattga agtttccccg
    22141 acgattacac tgccacagca ggatccaagc ctgatgggcg tcaccgtaag cattaatggt
    22201 gccgatcagc gtacagatca ggcgctggca aaagtcaccc gcgataatca aatcgttacc
    22261 ctgaccgcct cccgcgatct gcgtttcctg ctgcaagaag tgctggaaaa acagatgacc
    22321 gcgcgtggtt acatggttgg tccgaatggc ccggttaatc tgcaaatcat cgttagccaa
    22381 ctgtatgctg acgtgtccca gggcaacgtg cgctacaaca tcgctaccaa agcggatatc
    22441 gccatcatcg ctaccgcgca gaatggcaac aaaatgacca aaaactatcg tgccagctac
    22501 aacgttgaag gtgcgttcca ggcctccaac aaaaatatcg ccgatgcggt taacagtgtg
    22561 ctgaccgata ccatcgctga tatgtctcag gacaccagca ttcacgaatt catcaagcag
    22621 aacgcgcgtt aatttctgcc ctctggcccg gtgcaagccg ggcctgtaga cgcccatgtc
    22681 cagtcaatat ttacgtattt ttcaacagcc gcgttcagcc atattgctga tcctgggttt
    22741 tgcttccggg ctaccgctcg ccctgacatc cggcacctta caggcctgga tgacggtcga
    22801 aaatatcgat ctcaaaacca ttggtttctt ctctctggta ggccaggctt acgtttttaa
    22861 attcctctgg tcaccgctga tggaccgcta cacgccccca ttttttgggc ggcggcgcgg
    22921 ttggctgctc gccacgcaaa tcctgttatt agtcgccatt gcggcgatgg gttttctcga
    22981 accaggcacc caactccgct ggatggcggc gctggcggtg gtgatcgctt tttgctctgc
    23041 ctcccaggat attgtcttcg atgcgtggaa aaccgatgtg ctcccggcag aagaacgtgg
    23101 tgcgggcgcg gcaatcagcg tgctgggtta ccgtttaggg atgctggttt ccggcggcct
    23161 agccctgtgg ctggcagata aatggttggg ctggcagggc atgtactggt tgatggcggc
    23221 gctgttgatc ccctgtatta tcgcgacgtt gcttgcacca gaaccaaccg acaccattcc
    23281 tgtgcccaaa acgctggaac aagcggttgt tgcacctctg cgagatttct ttggtcgcaa
    23341 taatgcctgg cttattttgc ttcttatcgt gctgtataag ctgggcgacg cattcgccat
    23401 gagcctgaca accacgtttt tgattcgcgg cgtcgggttt gatgcgggtg aagtaggcgt
    23461 ggtaaacaaa acgcttggct tactggcgac cattgttggc gcattgtacg gtgggatttt
    23521 gatgcagcgc ctgtcgctgt tccgggcgct gctgattttc ggcattttac aaggtgcgtc
    23581 taacgctggt tactggctgc tgtcgattac tgataagcat ctctacagca tgggcgcagc
    23641 cgtctttttc gaaaacctct gtggcgggat gggcacatca gcctttgtcg cgctgttaat
    23701 gacgctgtgt aataagtcat tttccgctac tcaatttgcc ctgctctcag cgctttccgc
    23761 tgtagggcga gtttatgtcg gccccgtggc gggttggttt gttgaagcac acggctggtc
    23821 gacattctat ctattctccg tcgccgctgc cgtaccgggg cttattttgc tgctggtttg
    23881 ccgccagacg cttgaatata cacgagtaaa tgacaacttt atctcccgta ccgaatatcc
    23941 ggcaggttat gcctttgcca tgtggacact ggcggcgggc gtcagcctgt tggccgtgtg
    24001 gttacttctg ttgacgatgg acgcgctgga tttgacgcac ttctctttcc tgcctgctct
    24061 gctggaagtc ggggttttag tcgccctttc tggcgtcgtg cttggtggtt tgctggatta
    24121 tctggcgcta cgaaaaacgc atctgacgta atctgtaaat attatttagc tgtagtaatc
    24181 actcgccgta attattacgg ctaaataaac atcagcatcg cttattaata tttgttcttt
    24241 tgtgcggctt agcgtttgga aattattggc gccatttata aattctattt ttcttacacg
    24301 attcagctaa tgagtcttta ttttctcacc acccagttgt cactctaatg ataattattt
    24361 gttaaataat tgttttattt cgcattggtt ataccaattg cccgccaaga ctccttctgc
    24421 actccccttt tgttat
//
LOCUS       DAJTRQ010000049        24407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000049 DAJTRQ010000000
VERSION     DAJTRQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..943
                     /locus_tag="OO332_002075"
                     /pseudo
     CDS             1..943
                     /locus_tag="OO332_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1336..1677)
                     /locus_tag="OO332_002076"
     CDS             complement(1336..1677)
                     /locus_tag="OO332_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5942559.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1690..2562)
                     /gene="copD"
                     /locus_tag="OO332_002077"
                     /pseudo
     CDS             complement(1690..2562)
                     /gene="copD"
                     /locus_tag="OO332_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(2566..2940)
                     /gene="yobA"
                     /locus_tag="OO332_002078"
     CDS             complement(2566..2940)
                     /gene="yobA"
                     /locus_tag="OO332_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5942560.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3079..3309
                     /gene="holE"
                     /locus_tag="OO332_002079"
     CDS             3079..3309
                     /gene="holE"
                     /locus_tag="OO332_002079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5942561.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3411..4067
                     /locus_tag="OO332_002080"
     CDS             3411..4067
                     /locus_tag="OO332_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5942562.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4091..4753
                     /gene="exoX"
                     /locus_tag="OO332_002081"
     CDS             4091..4753
                     /gene="exoX"
                     /locus_tag="OO332_002081"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5942563.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4750..6810)
                     /gene="ptrB"
                     /locus_tag="OO332_002082"
     CDS             complement(4750..6810)
                     /gene="ptrB"
                     /locus_tag="OO332_002082"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5942564.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7019..7678)
                     /locus_tag="OO332_002083"
     CDS             complement(7019..7678)
                     /locus_tag="OO332_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5942565.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8005..8361)
                     /locus_tag="OO332_002084"
     CDS             complement(8005..8361)
                     /locus_tag="OO332_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5942566.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8428..8718)
                     /gene="yebG"
                     /locus_tag="OO332_002085"
                     /pseudo
     CDS             complement(8428..8718)
                     /gene="yebG"
                     /locus_tag="OO332_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            8852..10030
                     /gene="purT"
                     /locus_tag="OO332_002086"
     CDS             8852..10030
                     /gene="purT"
                     /locus_tag="OO332_002086"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5942567.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10085..10726)
                     /gene="kdgA"
                     /locus_tag="OO332_002087"
                     /gene_synonym="eda"
     CDS             complement(10085..10726)
                     /gene="kdgA"
                     /locus_tag="OO332_002087"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5942568.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10763..12574)
                     /gene="edd"
                     /locus_tag="OO332_002088"
     CDS             complement(10763..12574)
                     /gene="edd"
                     /locus_tag="OO332_002088"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5942569.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12809..14284)
                     /gene="zwf"
                     /locus_tag="OO332_002089"
     CDS             complement(12809..14284)
                     /gene="zwf"
                     /locus_tag="OO332_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5942570.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14622..15491
                     /gene="hexR"
                     /locus_tag="OO332_002090"
                     /gene_synonym="yebK"
     CDS             14622..15491
                     /gene="hexR"
                     /locus_tag="OO332_002090"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5942571.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15619..17061
                     /gene="pyk"
                     /locus_tag="OO332_002091"
     CDS             15619..17061
                     /gene="pyk"
                     /locus_tag="OO332_002091"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5942572.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17192..18163)
                     /gene="lpxM"
                     /locus_tag="OO332_002092"
                     /gene_synonym="msbB"
     CDS             complement(17192..18163)
                     /gene="lpxM"
                     /locus_tag="OO332_002092"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5942573.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18283..19605)
                     /gene="mepM"
                     /locus_tag="OO332_002093"
     CDS             complement(18283..19605)
                     /gene="mepM"
                     /locus_tag="OO332_002093"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5942574.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(19621..20553)
                     /gene="znuA"
                     /locus_tag="OO332_002094"
     CDS             complement(19621..20553)
                     /gene="znuA"
                     /locus_tag="OO332_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5942575.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            20632..21387
                     /gene="znuC"
                     /locus_tag="OO332_002095"
     CDS             20632..21387
                     /gene="znuC"
                     /locus_tag="OO332_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5942576.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21384..22169
                     /gene="znuB"
                     /locus_tag="OO332_002096"
     CDS             21384..22169
                     /gene="znuB"
                     /locus_tag="OO332_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5942577.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22316..23326)
                     /gene="ruvB"
                     /locus_tag="OO332_002097"
     CDS             complement(22316..23326)
                     /gene="ruvB"
                     /locus_tag="OO332_002097"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5942578.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23335..23946)
                     /gene="ruvA"
                     /locus_tag="OO332_002098"
     CDS             complement(23335..23946)
                     /gene="ruvA"
                     /locus_tag="OO332_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5942579.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24085..24150)
                     /gene="yobI"
                     /locus_tag="OO332_002099"
     CDS             complement(24085..24150)
                     /gene="yobI"
                     /locus_tag="OO332_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5942580.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24221..>24407
                     /locus_tag="OO332_002100"
     CDS             24221..>24407
                     /locus_tag="OO332_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942581.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTIT"
BASE COUNT         6008 a         6200 c         6004 g         6195 t
ORIGIN      
        1 gcgcaggcaa ttgaagcaaa tcattatata tacaaaaact actctcctgc tgccctaatt
       61 gaaaagctta aaggggtcga ctcatttact atggcagact ggattgaacg ttacaaaacg
      121 attcttataa ggagaaaagc gtccagaaat acttataaaa ctcgggtaaa tcaactggtg
      181 acaataaaag aaaaattagg aggggtttta ctgacagaaa taaccactcg ccatatcgcc
      241 gagtttcttg atttgtggat tgaaggaggg aaaaacacga tggcaggatc aatgcgttct
      301 gtgttatctg atatgttccg cgaagccatt gttgaaggac gtatatctca aaatccagta
      361 acgccaacaa gagcaccgaa aatagtagtt acaagagaac ggctgaaact aaagacatac
      421 aactgcatca gggaggcagc agatcaactt ccggcatggt tcccattagc tatggactta
      481 gcccttgtaa caggacaacg tcgcgaagac ataacgaata tgcggttcag tgatatttat
      541 gatgatcgtc tccacatcag gcaaattaag acaggaatga tgattgctat ccccctgtca
      601 ctcagccttc ctgtcgctgg tttacgactt ggtgcagtag ttgaacagtg ccgcatggta
      661 agtagggggg gattatctaa tcagtgccgg gattagaaaa aacagccctg acggcagcat
      721 tcacccggac ggcctgacaa agaaatttgt cgcagccaga aaattaacag gtatcctgtt
      781 cagtgaaaac ccaccaactt ttcacgaaat cagaagcctg gctggacgat tgtacaaaga
      841 aatatgtgga gaagaatttg ctcagcgtct acttggccac acatcggaga agacaacaaa
      901 aatgtatctt gatgagagag aaaaaacgta cttactgctc tgattttaac gtaaatggat
      961 tgttaaatgt attttggttg tgatataacc aaaaaagacc ggaatacaga aattcgagta
     1021 aatttcgggg aatttcgggg agacgtttgc aactaattga ttttaaatac aattaaaaaa
     1081 agaccgaata cgattcctgt attcggtcca gggaaatggc tcttgggaga gagccgtgcg
     1141 ctaaaagttg gcattaatgc aggcttagtt gccttgccct ttaagaatag atgacgacgc
     1201 caggttttcc agtttgcgtg caaaatggtc aataaaaagc gcggtggtca tcagcttaaa
     1261 tgttaaaaac cgcccgttct ggtgaaagaa ctgaggcggt ttttttattg gaaatcaaaa
     1321 ggctatttta ggtaattaac agagtttttc agctcgttct ataaacggtg ccagactcat
     1381 tttttcgccg ggattgttag gatcatcaat ctgaatcacc gaaatgggtt gggcattggt
     1441 cttcccactg gcaacttcct tttgtgcgat atcgtttaaa ggatactgca cgagggtact
     1501 tgggttgatg acatacaaag cattacccgg tcggcaagtc agcattacct cttcgcgatt
     1561 aaacgcccat ttgtctttac ccacttcaaa acgactcacg gttatcacct gtggagccgc
     1621 cagcgccgct gcagaactgg tgagtaacag aaacgccaga atactttttt tcatcatttt
     1681 cgtaatccat caaaaaggtt cgagagtcgc aagcaggctg atgatcacca gcactaccgc
     1741 acctatcgcc cattcgagtt tagtcatcca cacaaaccag ggtgccgcac gatcttcatc
     1801 ctgcctcatg cgcggtacaa gaacataacg attagccagc gcaatgacca ccataatcat
     1861 caccaggatg gctttgagca ataagagttg gccctagtaa gtcgtaagcg tggggggaaa
     1921 tccggtaatc aacaatgcat taagcacgcc gcttgccagt acgccgatca ccgcaaaatg
     1981 cccgcaccag gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac ggcctttgat
     2041 gagttgcata caccagagca ccggcagtaa tcccccaaac cacgccgccg cacaaatcag
     2101 gtgaatcgcg tgattagtct gatggatttt cgctgttacc ccttcattca gcgtcgcatg
     2161 ccccactccc gccagcagaa taaattgcgc ggtagtgagc ataaacaaca aacgtggcat
     2221 attccgcggt tgcataaggg cgacgatcaa cgttacgagg gcgagaacaa tttgccataa
     2281 ccagacgcca ccaaactgcg tttgcaaaac agcctgccaa atgtttggcg aaaatacatc
     2341 tgtccatccc gttcccatca acccgccctg aacggcgagc attgcagtgg cactgatcaa
     2401 actccagacg gcagcgtgct gttgtaaacg cagagaacgc ttcgtcagca agcgacgaat
     2461 cattaagggg gccagccagg caccatacat tgcaaagcca aagaccagca tcaacgaggt
     2521 gaaatggata aatcgcagcg cgatccaggt aaacgccagc atgatttatt tcacgctaaa
     2581 ggtgtagtgt cctttcgttt tatgcccgtc caccgaaaca acatgccagt caacggtgta
     2641 ggttccgggt ttcagcgaat cggccagtgg aacaatcagt tgcttttggt cctgctcatt
     2701 ccgcttcgct ggtaatgttt taatattttc gttttttggc cccgtgattt ttgcaccact
     2761 gaatcctgtt tcaacgcctt ccgagaagtt taaagtgatt gcctgcgggg cagctgtcac
     2821 ttgcgcgttt gccgcaggat actgatgcgt taaatgcgca tgtgcccaga cggaaggtgt
     2881 caccaacgaa gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag ttgaagccat
     2941 attatctatt cctttttgta ataacttttt tacagagcat aaccttgtct aatgtctgag
     3001 tcgaggatca tcaattccgg cttgccatcc aggctcactc ttagtaactt ttgcccgcga
     3061 atgatgagga gattaagaat gctgaagaat ctggctaaac tggatcaaac agaaatggat
     3121 aaagtgaatg tcgatttggc ggcggccggg gtggctttta aagaacgcta caatatgccg
     3181 gtgatcgctg aagcggttga acgtgaacag ccagaacatt tgcgcagctg gtttcgcgag
     3241 cggcttattg cccaccgttt ggcttcggtc aatctgtcac gtttacctta cgagcccaaa
     3301 cttaaataaa acttatacag agttacactt tcttacataa cgcctgctaa attatgagta
     3361 ttttctaaac cgcccccata atttgcagtc attttgaaaa ggaagtcatt atgtcgttct
     3421 ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc actcaccgaa caggtagctc
     3481 atcatctgga gtttgtgagg gccgccgcca ggcaacaatg tcagctgtta gtttttccat
     3541 cgctttcttt actggggtgt gattattcgc gacgtgccct tcctgcccca cccgatcttt
     3601 cactgttaga cccgctttgt tatgccgcaa cgacctggcg aatgaccatc attgccggcc
     3661 ttcccgttga atataacgat cgctttattc gtggaattgc ggtgttcgca ccgtggcgaa
     3721 aaacgcctgg gaactaccat cagagtcatg gtgcatgtct gggccgtcgt tccagaacca
     3781 tcacggtggt cgatgaacaa ccgcaaggta tggatatgga cccgacctgt tcactgttta
     3841 cgacagggca atgtctggga gaacccgacc tgctggcgtc ggcccgtcgc ttacaatttt
     3901 tttcacatca gtactcgatt gccgtgctga tggcaaatgc ccgtggtaac agtgcactat
     3961 gggatgaata tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg ttagtcggtc
     4021 agcgttcatc acagggttgg caaggcgata tcattccatt acgctaggct ttttcggcct
     4081 ggagcatgcc atgttgcgca ttatcgatac agaaacctgc ggtttgcagg gagggatcgt
     4141 tgagattgcc tctgttgatg tcattgacgg aaaaatcgtc aaccccatga gcctcctggt
     4201 gcgccccgat cgtcctatta gtccacaagc gatggcgatt catcgcatca ccgaagccat
     4261 ggtcgccgat aaaccgtgga ttgaagatgt gatcccacac tattacggta gtgaatggta
     4321 tgtcgcgcac aacgccagct ttgaccgccg cgtactgcct gagatgcccg gtgagtggat
     4381 ttgcactatg aaactggccc gtcgtttgtg gccagggctc aagtacagca atatggcgtt
     4441 atataaaaca cgcaagctca atgtacagac accgccgggc ctgcatcatc accgcgcgtt
     4501 gtatgactgt tatatcaccg ccgcgttgct tatcgatatt atgaacacct ccggctggac
     4561 ggcagaacag atggccgata tcaccggacg tccgtcgttg atgacgacct tcacctttgg
     4621 caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct atctgcgctg
     4681 gttatttaat aacctggaca gcatgagccc ggagctgcgt ttaacactga aacattatct
     4741 ggaaaatact taatccgcag gcgtagtggg taatgttccc tgcgccagcg cgaccagaaa
     4801 agcatattcc atcgctacgc cttcgtacga tttaaagcgg ccagatttac cgccatggcc
     4861 tgagtccatg tcggtacaga gcaataaaag atggtcatcg gttttcagct cgcgcaattt
     4921 agcgacccat tttgccggtt cccaatattg cacctgagaa tcgtgtaaac cggtcgttac
     4981 cagtaaatgc ggataagcct gtgcggtgac gttgtcatac gggctgtagc ttttcatgta
     5041 ctcgtaatat tgcggatcct gcggatttcc ccactcttca aactcaccag tggtaagagg
     5101 aattgattca tcaagcatcg ttgttacaac atcaacaaac ggtacctggg cgataacgcc
     5161 gtggaataat tccgggcgtt gattaattgc aacgcccatc aacatgcccc ccgcacttcc
     5221 gcccatcgca taacagagcg aaggagagcc atagcccagt tttaacaatg catcgcaggc
     5281 atcaagataa tcattaaacg tatttttctt cttcagaaat ttaccgtctt cgtaccattg
     5341 ttgccccagc tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc cacgatctaa
     5401 caaactcaag cggctaaaac tgaaatcggc atcaatactt gcgccgtaag aaccatagcc
     5461 atacaccagc aacgggttgt gtcctttgcg aaaatgtttg cgatgataga ccagcgaaac
     5521 cggaacttcg acgccatcac gggcgactat ccacaggtgt tcactgcggt aattcgccgc
     5581 atcaaaaccg ggcacttctg tttgttttaa tacacgacgc tcaccggtat ccatatccag
     5641 ttcaaacaaa gtgtctggtg tggtcatgga agaataacca taacgcaatc gcgcggtttc
     5701 aggttctgga ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg caatgccaat
     5761 gacttcccgg gtcttgcggt taatttggcg caaactggtt aacccgcgct gacgctcttc
     5821 aaccaccagc cagtcggtaa acagcgtaaa cccttccagc atgatgtttt cacgtggcgg
     5881 aattaactct tcccactgtt gctcatcacg catacgggta cggtataagc caaagttttt
     5941 gccgtggcga ttggaacgca gataaaaacg atgctggtag tgatcaaggc tgtattcgtg
     6001 atctttgcgg cgcggcagaa aaacaaacgg ctcggcatcg gccatttccg cgtccagcag
     6061 gcgaacttca ctggtggtgg cgctggccag atgaatgacg acatagtgct tcgacgtcgt
     6121 tttatgcagg ctgacataat aggtatcgtc tttttcttcg tagatcagtg tatcttgcga
     6181 tgctggcgta ccgatggcgt gacgccagac ctgataaggc agcagcgtca ccggatgctt
     6241 gcgaacatag tagaaagtcc aggagtcatt tgcccagaca aagctgggtt caacgttatc
     6301 cagcagttcc gggtaccagt tacctgtttc cagattacga aaacgaatgc cgtactggcg
     6361 tcgggaaaga aaatcttctg ccagcgccat aatggtgtta tcgggcgtaa tcgccattcc
     6421 gcccatcgaa taaaactcac tatgagctgc gcgcttgttg gcatcgagca atgtttccca
     6481 ctcatcccac tcttcgctga atgccgattg acgctggtag atagcatatt cacagcctgg
     6541 ttcataaata tgccgatagc ggtagccatt ttttatgtag ggcgcagaaa cttctctttg
     6601 cggaatgcgg tcgatgattt cctttaagat gcgatcctgc aaggcttgtt gtgaggccat
     6661 cacccgatga ccgtaactat tttcttgttg caggtagtcc aggacttctg gctgagaacg
     6721 cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta tcgccatgaa gcgtcatggc
     6781 gtggggaatg cgggcggctt ttggtagcat gttattgttc tttctggttg aaacatctta
     6841 taagggtggc aaaactcacc ggggatgcaa gcgaaacagg gtcgtcattg cttagatgat
     6901 gacaggtaat ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc ctgttcaaat
     6961 ggctgtgcgg ttctggatag cccgaaatag tcaacttcag gctatccaga gagcggaatt
     7021 attccgccaa agtgcgtttt tgctgttcga gatcgcgttc aatgccttca cgaacatcct
     7081 gggggatttt cagcgcgtca cccagtgcat tcaggtaact gcgttccata aaatggtcaa
     7141 tatcaatagc cgcgcagctc aggaaataga tttccagcgc ctcttcttca ttgcggaccc
     7201 cggtagccag gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg agtacacgcc
     7261 cctgctcttc cacgccggct tcacgcaatt gctggtcgat tgccgcacgt tcattggcat
     7321 caatatgacc atcactttta gcggcaaaga ccagcgcaag gatcaaacgt tccgtacgct
     7381 catccagcgg cgtactttgc gcgccaaact gcggttcgtc ctgatgcgcc gcgcgaattt
     7441 tatctttgta tttattccac agcaccgtac ccgctaccgc tccgccgcca accagtaacg
     7501 cgttggtgcc atatttcgta agaagtttac gtgctgattt atttgcgacc agcagcccag
     7561 ccagtccgcc taatgcgcct ggcactaaca gtttgcccaa tccctgatcc gcagacgagg
     7621 aggtagaaga actgctttgc ccaagcaggg attgcagttg atttaaccag ttagccatat
     7681 ttgctcctca ataaccattt ttagtgcctg tagcatagcg gatgaggatg tcagaaaatg
     7741 tatatcgaga caaaagatgc gcaaataagc ggggcgctag gccgttattc aaagaaattc
     7801 gcgccaggta aggtatttac tgtatatcag gtgataaggg tattttaggt gaaagtagtt
     7861 tgtcgttttg agtggcattg tttgatgctg attgcccgat gcgacgctgt tgcgtcttct
     7921 caggcctacg attgtggtgc gttctgggac ggacaaggca ctcgcgccgc atccgccaac
     7981 ggtcaatcga attatcagaa gccattaacg ccgctgatat tccgccattc ccgctttgca
     8041 gtccacgctg acctggtaat gaatatcggc acttttacca cgcacggtta gcggtactga
     8101 ccatttatca tctttaccct gaatgtcctg caaactgacc caggccacgg gatcggcctg
     8161 accgacaatt ttttgatcat ctgcccagcg cgccacgcga ttttgttgat aatcacgttt
     8221 tacgctcgcg gcaattccgg tagcatccag accttcacac tttgggaaag tgaccgactt
     8281 gctggtttca ttattggcag cgaaaactga tgcgcaggca gaaaccaaca accgccctaa
     8341 aaacgcccct ctttttttca tgtttttctc catagcacaa tgattcagga gaaagcatgg
     8401 tacaaattgt caggagcgca agttgcttca ggccgcgtgt gaggcatctt cctcttcgga
     8461 ttcagcaccg acgaccagtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcat
     8521 atctttttgt tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac
     8581 tggagaattt gtcagctagg tgtcaagcag atccgccgta tcaagcattt tgtcataagc
     8641 atcggcttcc tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta
     8701 tttaacttca accgccattt gcagcctctc ataataactg tgattttata cagtatattt
     8761 cttttcggtt gagaaatcaa catcagcaat aaagacacac gcaaacgttt tcgtttatac
     8821 tgcgcgcgga attaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc
     8881 ggcagcaact cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga
     8941 gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat
     9001 gcatgtcgcg catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt
     9061 ggttgaactg gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat
     9121 gctgatccaa cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg caacgaaatt
     9181 aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa gagctgcagc tgcccacttc
     9241 cacttatcgt tttgccgata gcaaaagcct tttccgcgag gcggttgccg ccattggcta
     9301 tccctgcatt gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt ttattcgctc
     9361 tgcagagcaa cttgctcagg catgggaata cgctcagcaa ggcggtcgcg ccggagcggg
     9421 ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc taaccgtcag
     9481 cgcggtggat ggcgtccatt tctgtgcacc agtaggtcat cgccaggaag atggcgacta
     9541 ccgtgaatcc tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg cgcaggagat
     9601 tgcccgtaaa gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg agctgtttgt
     9661 ctgtggtgat gaggtgattt tcagtgaggt ctcccctcgt ccacatgata ccgggatggt
     9721 gacgttaatt tctcaagatc tctcagagtt tgccctgcat gtacgtgcct tcctcggact
     9781 tcctgttggc gggatccgtc agtatggtcc tgcagcttct gccgttattc tgccacaact
     9841 gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag atttgcagat
     9901 tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt ctgggggtgg tactggctac
     9961 ttcagagagt gttgttgacg ccattgaacg cgcgaagcac gccgccggac aggtaaaagt
    10021 acagggttaa acccggcaaa aaacgctaca aaaatgcccg atcctagatc gggcattttg
    10081 acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaatg cggtcgtaat
    10141 cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt
    10201 tcagtgccag gtagtcacgg tagttagccg gagaaatacc acccgtcggg cagaaacgga
    10261 cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta gcttcagccg
    10321 ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc
    10381 tgatccccgg aatcagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca
    10441 gacccgggct aattgcgaac tgtgcacccg cttcagtgac ttctgccagc tgctgtggat
    10501 tcagcaccgt accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag
    10561 cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca
    10621 acgcttttgc catcggcacc gcgtgttcca gtttttttac cacgataacc ggtacaaccg
    10681 ggccggtggt caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc
    10741 gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg
    10801 acagtttttc acgcaaggcg ctgaataatt cacgtcctgt tctcacgcgt gacgcgctca
    10861 ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc gtctaccagc agtgtcagtt
    10921 cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc
    10981 catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga
    11041 gtcgtccatc ggtaactaac gcaattttga aacaccggtc caataataca ccaagtggcg
    11101 gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa
    11161 cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt
    11221 caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca gaggttttca
    11281 taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt
    11341 gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca
    11401 gttcaccatt attcagccat ggttcaaggg tataacgaaa cagaccaaag cctgccaccg
    11461 tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc
    11521 cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca
    11581 gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata ccggccgcgc
    11641 gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc aacagtgcaa
    11701 cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac
    11761 cattaccggt catgcgtgta acctgacgcg cagcggcagc ggtcaaagca tcgcgcagcg
    11821 gagaatccgg atgaacaaaa gaagagcctg gcaactgcat ccccataaat tccaccacca
    11881 tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg taagacgcgg
    11941 cttctgactc cagtaaggcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac
    12001 gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag
    12061 gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac
    12121 cgaggaacag agcaccatca aacatgttat gggacagccc caccgccgca gacatcgcta
    12181 tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg acaccatcac
    12241 acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc agggctttac
    12301 gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg
    12361 tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc
    12421 aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgacg
    12481 ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa cgttcaatga
    12541 ttcgatttgt tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag
    12601 agcaggatga ttcacaacgc gtttcattca gaggatttat gagtgaaacg cctgtaaccg
    12661 gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc
    12721 gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt gcaagattat gttaccggta
    12781 aaataaccat aaaggataag cgctgatatt actcaaactc attccaggaa cgaccatcac
    12841 gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg
    12901 catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca
    12961 cttcgtcgcg acgtacaaac agtgcctgaa taccacgcat ggtttccagc agcaaacgtt
    13021 cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga tccagcttgg
    13081 tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc tggatatcca
    13141 cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgact
    13201 ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg
    13261 gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt
    13321 cgacgcggat cgccacgaag gtttctgtat tgctactttt gttcgcgccc tcttcttcca
    13381 gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgcccg cgtacggttt
    13441 tttcgcgtac gttggagcgg tcgatgcggc gcagggactt cagtactttc actttttcat
    13501 cgcggatgct gtctgcgctc aggtcagatg gcggagacat cgcaatcatg cagagaattt
    13561 gtagcaggtg gttctgaatc atgtcacgca tctgaccggc tttatcaaaa tagccccagc
    13621 gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt
    13681 cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt
    13741 ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct
    13801 gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc
    13861 gtgccggttt agcattcagt tttgcctcgc caagcccttt gcaaattgcg ccaaaagtgc
    13921 tgggcggcat ggcaaagtag ttaatagtga tacgattttt ttgatccagc atcgcgccga
    13981 gacggttgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca
    14041 gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga
    14101 caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt
    14161 ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg
    14221 caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg
    14281 ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata cttgtaattt
    14341 tcttacggtg cactgtactg cttttccgag cttgcgaaaa ctgtaaacgc ttatccaccc
    14401 gtgcgattac gggaaaagcg cgcaaagtgc ggcaaaactg ataaaaaaat catcgttttc
    14461 cattcggtaa aaatctgaca ctgatcatgt tatgaaaaaa aataacaact tttttatctg
    14521 cttttgtcat taacacggca cacaggcgta atatctgaca aaactgcatt tcgatttctt
    14581 tcagtgcgga aatcgtcatt acccgtgagt ctctttacat catgaatatg ctggaaaaaa
    14641 tccagtctca gctggaacat ttgagcaaat cagagcgcaa agttgccgag gtcattctgg
    14701 cttcgcccga taacgcgatc cattcgagta ttgctgctct ggcgctggaa gccaatgtta
    14761 gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac gcgcggtttt cctgatttta
    14821 aacttcatct ggcacaaagt ctggcgaatg gcactcccta tgttaatcgc aacgtcaatg
    14881 aagatgacag cgttgaatca tacacgggga aaatatttga gtccgcaatg gcaacgctcg
    14941 atcatgtccg tcattcactg gataaatctg ccatcaaccg cgccgtcgac ttgctcactc
    15001 aggcaaaaaa aatcgccttt ttcggattag gctcttctgc cgccgttgcc cacgatgcga
    15061 tgaataagtt ctttcgtttt aatgttccgg tggtgtactc cgatgatatc gtgctgcaac
    15121 gcatgagttg tatgaattgt agcgacggag acgtggtggt gctgatttct cacacaggaa
    15181 gaacaaaaaa tctggtcgag ctggcgcagc tggcacgcga aaacgacgcc atggtgattg
    15241 ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt accctcgacg
    15301 taccggaaga tactgacatt tatatgccca tggtttctcg acttgcacag ctgaccgtga
    15361 tagatgtgct ggcgacagga tttactttgc gacgcggtgc aaaattcaga gataacttga
    15421 agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa gcagttactt aatttaagtg
    15481 acgatcgcta aaagcgactg tcactgtcct aatcttatac gacatccgaa tgagattaat
    15541 ttatcgccat cgcggcgtta tttcattcgg atttcatgtt caagcaacac ctggttgttt
    15601 cagtcaacgg agtattacat gtccaaaagg cttcgcagaa caaaaatcgt taccacgtta
    15661 ggcccagcaa cagatcgcga taataatctt gaaaaagtta tcgcggcggg tgccaacgtt
    15721 gtacgtatga acttttctca cggctcgcct gaagatcaca aaatgcacgc ggataaagtt
    15781 cgtgagattg ccgcaaaact ggggcgtcat gtggctattc tgggtgacct ccaggggccc
    15841 aaaatccgtg tatccacctt taaagaaggc aaagttttcc tcaatattgg ggataaattc
    15901 ctgctcgacg ccaacctagg taaaggtgaa ggcgacaaag aaaaagtcgg tatcgactac
    15961 aaaggcctgc ctgctgacgt cgtgcctggt gacatcctgc tgctggacga tggtcgcgtc
    16021 cagttaaaag tactggaagt tcagggcatg aaagtgttca ccgaagtcac cgtcggtggt
    16081 cccctctcca acaataaagg tatcaacaaa cttggcggcg gtttgtcggc tgaagcgctg
    16141 accgaaaaag acaaagcaga cattaagact gcggcgttga ttggcgtaga ttacctggct
    16201 gtctccttcc cacgctgtgg cgaagatctg aactatgccc gtcgcctggc acgcgatgca
    16261 ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg cagccaggat
    16321 gcaatggatg acatcatcct cgcctctgac gtggtaatgg ttgcacgtgg cgacctcggt
    16381 gtggaaattg gcgacccgga actggtcggc attcagaaag cgttgatccg tcgtgcgcgt
    16441 cagctaaacc gagcggtaat cacggcgacc cagatgatgg agtcaatgat tactaacccg
    16501 atgccgacgc gtgcagaagt catggacgta gcaaacgccg ttctggatgg tactgacgct
    16561 gtgatgctgt ctgcagaaac tgccgctggg cagtatccgt cagaaactgt tgcagccatg
    16621 gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca acgtttctaa acaccgtctg
    16681 gacgttcagt tcgacaatgt ggaagaagct attgccatgt cggcaatgta cgcagctaac
    16741 cacctgaagg gcgttacggc gatcattacc atgaccgaat cgggtcgtac cgcgctgatg
    16801 acctcccgta tcagctctgg tctaccaatt ttcgccatgt cgcgccatga acgtacgctg
    16861 aacctgactg ctctctatcg tggcgttacg ccggtgcact ttgatagcgc taatgacggc
    16921 gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata aaggttactt gatgtctggt
    16981 gacctagtga ttgtcaccca gggcgacgtg atgagtaccg tgggttctac taataccacg
    17041 cgtattttaa cggtagagta agtacgttgc cggatgcggc gaaaacgcca catccggcct
    17101 atagttcaat tatagttcaa cagatttcga atattctgaa gcaaacttga acttatcatc
    17161 aggcgaaggc ctctcctcgc gagaggcttt tttatttgat gggataaaga tctttgcgct
    17221 tatacggctg gatttcgccc ggtttgcgag ttttcagcaa ttttagtatc caggtgtatt
    17281 gttctggtcg cggaccaaca aaaatctcga cttcttcatt catccgccgc tcaatcgtat
    17341 gatcatccgc ctctaacaga tcatccatcg gtgggcgcac ctgaatcgtc agacgatgcg
    17401 tcttgccatc ataaatcgga aacagcggta caacgcgcgc acggcatact ttcatcaaac
    17461 gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa atccacaaat tcgctgtgct
    17521 ctgggccatg atcctgatcg ggtaaataat atccccagta cccctgacgt accgactgga
    17581 tgaatggttt aataccgtca tttctcgcat gcagacgacc gccaaagcga cgacgcaccg
    17641 tgttccagac ataatcaaaa accgggttgc cctgattatg gaacatcgct gccattttct
    17701 gcccttgcga ggccatcagc atggcaggaa tatcgacggc ccaaccgtgc ggcaccagaa
    17761 agataacttt ctcgttatta cgccgcatct cttcgatgat ctccagccct tgccagccaa
    17821 cgcgcggctg aattttctcc ggcccgcgta ttgccaactc agccatcatt gccatcgctt
    17881 gcggcgcggt ggcaaacatc tcatcaacaa tcgcttcgcg ttcagcttca ctacgttctg
    17941 gaaagcagag cgacagattg attaacgcac gacggcgtga gctttttccc agtcgtccgg
    18001 caaaacgtcc cagccgtgcc agaatgggat cacggaactt tggcggcgtt aaagcgatac
    18061 ccgccatcgc tgctacaccc agccatgctc cccagtagcg cgggtggcga aaggatttat
    18121 caaactcagg aatgtattcg ctattatttt ttttcgtttc catgcttttc cagtttcgga
    18181 taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac ttgccccgca ccaaataaaa
    18241 aaagccggta ctgactgcgt accggctgcg aatggatgtt aattaatcaa acagtagctg
    18301 cggcacaatc tctttggcct gcgccaggaa ttcgcgacga tcggagccgg tcagcccttc
    18361 ggtacgcggc agttttgccg tcagcgggtt tacggcctgc tggtttatcc atacttcata
    18421 gtgcagatgc ggcccagttg aacgtccggt attaccggaa agcgcgatac ggtcgccacg
    18481 tttcaccttc tgtcccggtt tcaccagaat cttgcgcaag tgcatataac gcgtggtgta
    18541 gctgcgacca tgacgaatag ccacataata acctgctgcg ccactgcgtt tggcaaccac
    18601 cacttcaccg tcacccactg aaagcactgg cgtaccttgt ggcatggcga aatcaacacc
    18661 tctgtgtggt gcaacgcgac ctgtcaccgg attagttcga cgcgggttaa agttagacga
    18721 gatacggaac tgtttcgccg tcgggaatcg caagaatcct ttcgccagac cagtaccgtt
    18781 acgatcgtag aatttgccat cctcagcgcg gattgcgtaa taatctttac cttctgaacg
    18841 caaacgtacg cccagcagct ggctttgctc acgtttacca tcaagcattt ctcgagacat
    18901 taataccgca aattcatcgc cttttttcag tttgcggaaa tccatttgcc actgcatggc
    18961 tttaatcact gcgctcactt cggcgctggt taaaccggcg tttctggcgc tggcaacaaa
    19021 gctccctccg acggtacctt tcagcagatt gttaacccac tctccttgct gcatttcgct
    19081 ggtcatttta aaaccgttag cggcagtacg gtcataggtt cgggtttcac gacgagacac
    19141 ttcccaggtg aggcgctgca gttcgccgtc cgcggttaat gtccaggaga gttgttgacc
    19201 gattttcagg ttacgcaatt ctttgtcggc agcagcgagt tgggtgatat cacccatatc
    19261 aataccatac tgattgagaa tgctgcttag cgtatcgcca gtggaaacaa catattcatg
    19321 cacgcccgct tcaccggcga ttttgtcatc cagttcgtcc tggggaatgg cttcatcttc
    19381 ttgtgcagct tgatcaatcg gctcactggc ttcaggtaag agcgaacgaa tttcgttctg
    19441 ttccagctca atggttttga caattggcgt ggcatcacgg tgataaacat agggccgcca
    19501 gacagcgacg gccagagtaa gaacggtgag cgaccccaac ataacgcggt gtggtcgcgg
    19561 taaattatta aacgccaggg cgacagagcg ggctatctgt tgcacgtaat cacttcctca
    19621 ttaatctcct ttcaggcagc tcgcatactg gttggctaat tgactcagga attctgaata
    19681 gcttgtttta cccagtttga tattcgtccc caggggatcc aacgttccca tacgaacgga
    19741 tgtccctcgt gcgacgctct caacgaccgc tggcctgaac tgtggctcag caaaaacgca
    19801 ggttgctttt tgctcaacca actgtgttct tatttcatgt aaacgctgcg cgccaggttg
    19861 aatctcaggg ttaacggtaa aatgaccaag cggtgtcagt ccgaactgtt tttcgaaata
    19921 gccgtaagca tcgtgaaaaa cgaaataacc tttccccttg agcggcgcga gctcgttacc
    19981 aacctgcgtt tcggttgagg ccaattgtgc ctcaaaatcc ttcaggttgg cgtcaagttt
    20041 ggctcgactt tgcggcataa gttccactaa ttttccatgg attgcaaccg ctgtagcccg
    20101 cgctatctct ggggaaagcc aaagatgcat gttgaaatcg ccgtgatggt gatcttcgtc
    20161 acttttttcc gcgtggtcgt gatcatcatc atcgccgtga atacttttca tcagtagcgg
    20221 tttcacatct tcaagctgcg caatcgttac ctgcttcgct tctggtaatt tgcttaccgg
    20281 tttttgcata aacgcttcca tctccgggcc aacccaaacg actaagtccg cgttctgtaa
    20341 gcgttttaca tccgatggac gcagtgaata atcatgttct gaagcgccgt caggcagtaa
    20401 aacctccgtt tctgttacac catcagcaat ggcagaagcg atgaacccaa cgggtttaag
    20461 cgaagcgaca acggcggcat ctgcggcctg tgttgcacct ccccagagag cggcggataa
    20521 tgctgcgaaa agaagcgttt ttttatgtaa cataatgcga ccaatcatcg taatgaatat
    20581 gagaagtgtg atattataac atttcatgac tactgcaaga ctaaaattaa catgacaagt
    20641 ctggtttccc tggaaaatgt ctcggtttct tttggccaac gccgcgtcct ctctgatgtg
    20701 tcgctggaac ttaaacctgg aaaaattttg actttacttg ggccaaatgg cgcaggtaag
    20761 tcgacactgg tacgggtagt gctcgggctg gtaacacccg atgaaggggt tatcaagcgc
    20821 aacggaaaac tgcgcatcgg ctatgtaccg cagaagctgt atctcgacac cacgttgcca
    20881 ctgaccgtaa accgtttttt acgcttacgc cctggtacac ataaagaaga tattttgcct
    20941 gcactgaaac gtgtccaggc cgggcacctg attaacgcac cgatgcaaaa gctctctggt
    21001 ggcgaaacgc agcgtgtact attagcgcga gcattgttaa attgcccgca attattagtg
    21061 ctggatgaac ccactcaagg cgtggatgta aatggccaag tggcgttata tgaccttatt
    21121 gaccagttgc gtcgtgaact ggattgtggc gttttaatgg tttctcacga tctgcatctg
    21181 gtgatggcaa aaaccgatga agtgctgtgc ctgaatcacc acatttgttg ttccggcaca
    21241 ccggaagttg tttccctgca tccggagttt atttcaatgt ttggtcctcg tggtgctgaa
    21301 caactgggta tctatcgcca tcatcataat catcgtcacg atttacaggg acgaattgtt
    21361 ttgcgtcggg gaaatgatcg ctcatgattg aattattatt tcccggttgg ttagccggga
    21421 tcatgctcgc ctgtgccgcg ggtccgctgg gttcgtttgt agtctggcgt cgtatgtctt
    21481 atttcggtga tacgctggct catgcctcat tacttggtgt cgcgtttggt ttgttgctgg
    21541 acgtgaatcc attctatgcg gtgattgccg ttacgctgct gctggcgggc ggtctggtat
    21601 ggctggagaa gcgtccacag ctggcgatcg acacgttatt agggattatg gcgcacagtg
    21661 ccctgtcgct gggcctagtg gtcgttagtc tgatgtctaa cattcgtgtt gatttgatgg
    21721 cttacctgtt cggtgattta ctggcagtga cgccagaaga tctcatctct attgcgattg
    21781 gcgtggtcat cgtggtggct attttgttct ggcaatggcg caatttgctg tcgatgacga
    21841 ttagcccgga tctggcgttt gttgatggtg tgaaattaca gcaagtgaaa ttgttgttga
    21901 tgctggtgac ggcattgacg attggtgtag cgatgaaatt tgtcggtgcg ttgattatta
    21961 cttcgttgct gattattcct gcagctactg cgcgtcgctt tgcccgtacg ccggaacaga
    22021 tggctggtgt cgctgttttg gtggggatgg tggcagtaac tggcggttta accttttccg
    22081 cgttttacga tacgccggcg ggtccgtcgg tggtgctatg tgcggcactg ttatttatta
    22141 tcagtatgat gaaaaagcag gccagctaat ctatcgctga acacatttgt cggatgcggc
    22201 gcgagcgcct tatccgacct acggttcgct atctctggct ggcctgataa gacgcgaaca
    22261 gtgtcgcatc agacacactg ccagtgtcgg atgcggcgcg agcgaccaat ccgacttacg
    22321 gcatttctgg cggcgttatg ccaaagtgat tccacgcccg cgtcgtcgcc atacgcccgc
    22381 gcggtgtacg ctgcaaaaag ccttgctgaa tcaaataagg ttccagtaca tcttcaatgg
    22441 tttcacgttc ttcgccaatg gctgccgcca ggttatccag acctaccggc ccaccaaaga
    22501 acttatcgat taccgccagc aacaatttgc ggtccatata atcgaaacct tcagcatcga
    22561 cattcaacat atccagcgcc tgagcagcga tatctgccga gatggtgcca tcgtgcttca
    22621 cttcggcgaa atcacgcact cgacgcagca gacggttggc aatacgtggc gtaccgcgag
    22681 cgcgacgagc tacttcctgc gcaccgtcat cactcatctc aagccccata aagcgtgcac
    22741 tgcgactgac gatatattgc agatccggca cctgataaaa ctccagacgt tgcacaatac
    22801 caaaacggtc gcgcaacggt gatgtcagcg aacctgcgcg cgtggttgcg ccaatcaggg
    22861 taaacggcgg caaatcaatt ttaatggagc gtgccgccgg accttcacca atcatgatat
    22921 ccagttggta gtcttccatt gccgggtaca gcacttcttc aacaactggc gataaacggt
    22981 ggatctcatc aataaacagc acgtcatgcg gttcaaggtt agtgagcatc gcagccaaat
    23041 cgcccgcctt ttccagcacc ggaccagaag tcgtgcgtaa attaacgccc atttcattgg
    23101 cgacaatgtt ggcaagcgta gttttaccca accccggagg accaaaaatc aacaaatgat
    23161 cgagggcatc gccgcgcagt ttcgctgctt tgatgaaaat ctccatctgt gaacgaacct
    23221 gcggctgacc aacatactct tccagtaatt tagggcgaat ggcgcgatct gctacatctt
    23281 ccggcaaagt ggtaccggca gaaatcagac ggtctgcttc aatcatcctt tacctcataa
    23341 cgcggcgcgt agggcttcgc gaattaatgt ttcactgctg gcgtcagggc gagcgatttt
    23401 gctcaccatg cggcttgctt cttgtggttt atagcctagc gccaccagcg cggcaactgc
    23461 ttcttgttca gcatcgtcgg tcgccgggct ggcaggagac gtgagtacca ggtcggcggc
    23521 tggcgtaaag agatcgccat gcaaaccttt aaatcggtct ttcatttcaa caatcaagcg
    23581 ttcggcggtt tttttgccaa tacccggcag tttcaccagt gcccccactt cttcacgctc
    23641 aacggcatta acgaactgct gcgctgacat tccggagagg atcgccaggg ccaacttcgg
    23701 gccgacgccg ttggttttga tcaactcttt gaacaacgtg cgttcttgtt tattgttaaa
    23761 accgtacagc agttgcgcgt cttcacgcac cacaaagtgg gtgaaaacga tcgcttcctg
    23821 acccgcttca gggagttcat aaaaacaggt catcggcata tgcacttcat agcctacgcc
    23881 gcccacttca attaacacca gcgggggttg tttttcaatg atgatgcctc tgagtctgcc
    23941 tatcacatga cgctcctgcg taatgaatca aagataatgc tgtatgataa aaaaatgctg
    24001 gatagatatc cagcgtagga tgaagaaaac ttgcgaggtg tcccgatgat ctgaaaaatg
    24061 gcacagtata atttattcaa caggttatat tggaagcaaa tatttgaata ttacatattc
    24121 agtgaagaaa tgcgtaataa aaatatacat tgcgactcct gaaaaaataa tttttttatg
    24181 ctattacgta tattcgtatc tatttcaatg gaatgacaac gtgaatatta attaccctgc
    24241 tgaatatgaa attggagata tcgtctttac atgtataggt gctgccttat ttggtcaaat
    24301 atcagctgca tcaaattgct ggagtaatca cgtcgggatc attatcggtc ataacggtga
    24361 agacttcctg gttgcagaaa gccgggttcc cctttcaacc atcacca
//
LOCUS       DAJTRQ010000050        24335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000050 DAJTRQ010000000
VERSION     DAJTRQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..489)
                     /locus_tag="OO332_002101"
     CDS             complement(<1..489)
                     /locus_tag="OO332_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5942582.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLE"
     gene            762..950
                     /locus_tag="OO332_002102"
     CDS             762..950
                     /locus_tag="OO332_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5942583.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            962..1672
                     /gene="bcsQ"
                     /locus_tag="OO332_002103"
     CDS             962..1672
                     /gene="bcsQ"
                     /locus_tag="OO332_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5942584.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1669..4282
                     /gene="bcsA"
                     /locus_tag="OO332_002104"
                     /pseudo
     CDS             1669..4282
                     /gene="bcsA"
                     /locus_tag="OO332_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4293..6632
                     /gene="bcsB"
                     /locus_tag="OO332_002105"
     CDS             4293..6632
                     /gene="bcsB"
                     /locus_tag="OO332_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5942585.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6639..7745
                     /gene="bcsZ"
                     /locus_tag="OO332_002106"
     CDS             6639..7745
                     /gene="bcsZ"
                     /locus_tag="OO332_002106"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5942586.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7804..7980
                     /locus_tag="OO332_002107"
                     /pseudo
     CDS             7804..7980
                     /locus_tag="OO332_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8308..10056
                     /gene="bcsC"
                     /locus_tag="OO332_002108"
                     /pseudo
     CDS             8308..10056
                     /gene="bcsC"
                     /locus_tag="OO332_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10138..12126
                     /gene="hmsP"
                     /locus_tag="OO332_002109"
     CDS             10138..12126
                     /gene="hmsP"
                     /locus_tag="OO332_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5942587.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12310..13596
                     /gene="dctA"
                     /locus_tag="OO332_002110"
     CDS             12310..13596
                     /gene="dctA"
                     /locus_tag="OO332_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5942588.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13817..15313
                     /locus_tag="OO332_002111"
     CDS             13817..15313
                     /locus_tag="OO332_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5942589.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15409..16338)
                     /gene="kdgK"
                     /locus_tag="OO332_002112"
     CDS             complement(15409..16338)
                     /gene="kdgK"
                     /locus_tag="OO332_002112"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5942590.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16570..17337
                     /gene="pdeH"
                     /locus_tag="OO332_002113"
     CDS             16570..17337
                     /gene="pdeH"
                     /locus_tag="OO332_002113"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5942591.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17407..19467
                     /locus_tag="OO332_002114"
     CDS             17407..19467
                     /locus_tag="OO332_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5942592.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19649..20971)
                     /locus_tag="OO332_002115"
     CDS             complement(19649..20971)
                     /locus_tag="OO332_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5942593.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21382..22395)
                     /gene="yhjD"
                     /locus_tag="OO332_002116"
     CDS             complement(21382..22395)
                     /gene="yhjD"
                     /locus_tag="OO332_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5942594.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22444..23343)
                     /locus_tag="OO332_002117"
     CDS             complement(22444..23343)
                     /locus_tag="OO332_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5942595.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23863..>24335
                     /locus_tag="OO332_002118"
     CDS             23863..>24335
                     /locus_tag="OO332_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5942596.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGES"
BASE COUNT         5747 a         6130 c         6701 g         5757 t
ORIGIN      
        1 ctcaagcaac aatgaaaatt gtttatcgtt attatttccg ggattaatta ccaaaagcga
       61 acaatggttt aacctgctcc atttattcat tttatccaac catgagcgaa gccgctcggc
      121 aggaatgttt tgccatgcgt tatttgcgca aacaagaata aaaaggtaat tatggggatc
      181 aatagaacac tgcaaatcac gggccaaata gtatagacct ttttcatgat ttagcattga
      241 aaataatttt attttttccg gcccttgaga atcatctaat tgaaagattt tcgccggatc
      301 gctgtccatg ctaatgaccg cgacgtgtgc ggtttcagcc tgggatgcaa ttgtttgatt
      361 cgccagactg atagcatctt catggcgatc gacgttaaac caccagacgc cgcctgctgg
      421 catatgtcgc agctcatccc ataatgatga gataccgata gagaatacag ggtccacaat
      481 gtccctcatg tcaccatttt gtctgattat cagtttacta gcgaaagcta agaaataaac
      541 ctaccattga aattaaaact tatcagattt agcatgtgaa tcaatttaga aggtcacaag
      601 acgtgtgatt ttagtctttt cgtttttaac tttttctgtt gcgtaacgga aagtcaaaaa
      661 gtgagcaaat tcccgtctcg ccgtaaacaa taaaccggcg aaatgctcac ggttagaatt
      721 gtatcatgaa tggtacggtt atttcatagg gatcaagcaa aatgaataac aatgaaccag
      781 atactctgcc tgatcccgcg ataggctata tcttccagaa tgatattgtg gcgttaaagc
      841 aggcattttc actgcctgat attgattatg ccgatatttc ccaacgcgaa cagttggccg
      901 cggcattaaa acgctggccg ttgctggcag agtttgcgca acaaaagtag gggattggtg
      961 aatggccgta ctgggattgc agggggtgcg gggaggcgtg gggacaacaa ccatcaccgc
     1021 cgcattagcc tggtcattac aaatgttggg agaaaatgtc ctagtggtcg atgcctgccc
     1081 ggacaacttg ttgcgcctgt catttaatgt tgattttacc caccgtcagg gctgggccag
     1141 agcgatgctg gatggccagg actggcgtga cgctgggttg cgctacacct cgcagctcga
     1201 tttgctgcct tttggtcagt tatccattga agaacaagaa aatccacagc actggcaaac
     1261 ccggctgagc gatatttgct ccggcttaca gcaactaaaa gccagcgggc gttaccagtg
     1321 gattttaatc gacttaccgc gtgatgcctc gcagataacc caccagctgc tgagtttgtg
     1381 cgagcactcg ctggcaatcg tcaatgtgga tgccaactgc catatccgac tgcatcagca
     1441 agcgctgccg gatggcgcac atattttgat taatgacttc cgtattggca gtcaggttca
     1501 ggacgatatt taccagcttt ggttgcaaag ccagcgccga ttactgccga tgctcattca
     1561 tcgtgatgaa gcgatggctg aatgcctggc ggctgaagag atactgacgc tggcgaactg
     1621 gtgcctgttg aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg
     1681 acccggtggt tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat
     1741 cgtcgtcacg gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc
     1801 tggattttta ttccgctgga gcacccgcgc tggcaacgta ttcgcgcaga acataaaaac
     1861 ctgtatacgc caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat ctcattcaaa
     1921 catgctggtt attgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc agcgggcatt
     1981 ttcaggtctg caaaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg
     2041 cgttagccat aaaacacagc atctggatga aaaaaaagag ctcggtcatt tgagtgccgg
     2101 ggcgcggcgg ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg
     2161 cgttactcag ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggggt
     2221 agcgctgatc gtacggcgga tgccggggcg cttctcggcg ctaatgttga ttgtgctgtc
     2281 gctgaccgtt tcttgccgtt atatctggtg gcgttacacc tctacgctga actgggacga
     2341 tccggtcagc ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg cgtggattgt
     2401 gctggtgctc ggctacttcc aggtagtatg gccgctgaat cgtcagccgg tgccattgcc
     2461 gaaggatatg tcgctgtggc cgtcagtgga tatctttgtc ccgacttaca acgaagatct
     2521 caatgtggtg aaaaatacca tttacgcctc gctgggtatc gactggccga aagacaaact
     2581 gaacatctgg atcctcgatg acggcggcag ggaagagttt cgccagtttg cgcaaaacgt
     2641 gggggtgaaa tatatcgccc gcaccactca tgaacatgcg aaagcgggca acatcaacaa
     2701 tgcgctgaaa tatgccaaag gcgagttcgt gtcgattttc gactgcgacc acgtaccaac
     2761 gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc tggcgatgat
     2821 gcagactcca caccacttct tctcgccgga cccgtttgaa cgcaacctgg gacgttttcg
     2881 taaaacgccg aacgaaggca cgctgttcta tggtctggtg caggatggca acgatatgtg
     2941 ggacgccact ttcttctgcg gttcctgtgc ggtgattcgt cgtaagccgc tggatgaaat
     3001 tggcggcatt gctgtcgaaa ccgtgactga agatgcgcat acttctctgc gtttgcaccg
     3061 tcgtggctat acctccgtgt atatgcgtat tccgcaggcg gcggggctgg cgaccgaaag
     3121 tctgtcggcg catatcggtc agcgtattcg ctgggcgcgc gggatggtac aaatcttccg
     3181 tctcgataac ccgctcaccg gtaaaggact gaagtttgct cagcggctgt gctacgtcaa
     3241 cgccatgttc cacttcttgt cgggcattcc acggctgatc ttcctgactg cgccgctggc
     3301 gttcctgctg cttcatgcct acatcatcta tgcgccagcg ttgatgatcg ccctattcgt
     3361 gctgccgcat atgatccatg ccagcctgac caactcgaag atccagggca aatatcgcca
     3421 ctctttctgg agtgaaatct acgaaacggt gctggcgtgg tatatcgcac caccgacgct
     3481 ggtggcgctg attaacccgc acaaaggcaa atttaacgtc accgccaaag gtggactggt
     3541 ggaagaagag tacgtcgact gggtgatctc gcggccctac atcttccttg ttctactcaa
     3601 cctggtgggc gttgcggtag gcatctggcg ctacttctat ggcccgccaa ccgagatgct
     3661 caccgtggtc gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg gcggcgcagt
     3721 tgcggtatcg gtagaaagca aacaggtacg ccgatcgcac cgcgtggaga tgacgatgcc
     3781 cgcggcaatt gcccgcgaag atggtcacct cttctcgtgt accgttcagg atttctccga
     3841 cggtggtttg gggatcaaga tcaacggtca ggcgcagatt ctggaagggc agaaagtgaa
     3901 tctgttgctt aaacgcggtc agcaggaata cgtcttcccg acccaggtgg cgcgcgtgat
     3961 gggtaatgaa gttgggctga aattaatgcc gctcaccacc cagcaacata tcgattttgt
     4021 gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg caggacagct acccggaaga
     4081 taagccgctg gaaagtctgc tggatattct gaagctcggc ttccgtggct accgccatct
     4141 ggcggagttt gcgccttctt cggtgaaggg catattccgt gtgctgactt ctctggtttc
     4201 ctgggttgta tcgtttattc cgcgccgccc ggagcggagc gaaacggcac aaccatcgga
     4261 tcaggctttg gctcaacaat gatgataacg cgatgaaaag aaaactattc tggatttgtg
     4321 cagtggctat ggggatgagt gcgttcccct ctttcatgac gcaggcgacg ccagcaacgc
     4381 aaccactgat caatgctgag ccagctgtag ccgcccagac ggaacaaaat ccgcaggtgg
     4441 ggcaagtgat gtcaggcgag cagggagctg atgcccccat cgtggcgcag aacggtcctt
     4501 cgcgtgatgt gaagctgacc tttgcgcaaa tcgcgccgcc gccgggcagc atggtgctac
     4561 gtggtattaa cccgaacggc agcattgagt ttggtatgcg cagcgatgaa gtggtgacga
     4621 aggcgatgct caacctcgaa tacacgccat cgccatcgtt actgcctgtc cagtcgcagt
     4681 taaaggttta tctcaatgat gagctgatgg gcgtgctgcc agtgaccaaa gaacagttgg
     4741 gtaaaaaaac gctggcgcaa atgcccatta atccactgtt tattaccgac ttcaaccgtg
     4801 tgcggctgga gtttgtcggc cattatcagg acgtgtgcga aaacccggcc agcaccacgc
     4861 tttggctgga tgttgggcgg agcagtggac tggatctgac ctatcagacc ctgaatgtga
     4921 agaatgacct gtcacacttc ccggtgccat tctttgaccc gcgcgataac cgtactaaca
     4981 ccttgccgat ggtctttgcg ggtgcgccgg atgttgggct gcaacaagca tctgccattg
     5041 tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca gaacttcccg gtgctctata
     5101 accaattgcc ggatcgcaat gccattgtct ttgcaaccaa cgacaaacgg ccggacttcc
     5161 tgcgcgatca tccggcggta aaagccccgg tgattgagat gattaaccat ccgcagaatc
     5221 cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa agacctgttg caggcagcga
     5281 aaggtatcgc tcagggtaac attctgttcc gtggtgaaag cgtggtagtg aatgaagtga
     5341 aaccgctgct accgcgtaag ccgtacgatg cgccgaactg ggtacgtacc gatcgtccgg
     5401 tcacatttgg cgaactgaaa acctatgaag aacagttaca atccagcggc cttgagccag
     5461 cagcgattaa cgtttcgcta aacctgccgc cggatctcta cctgatgcgc agtaccggca
     5521 ttgatatgga tattaattac cgctacacca tgccgccggt gaaagacagt tcgcggatgg
     5581 atatcagcct gaataaccag ttcctgcaat ccttcaacct gagcagcaaa caggaggcga
     5641 accgtctgct gctgcgtatt ccggtattac aaggtttgct ggatggcaaa acagatgtct
     5701 ctattccggc gctgaaactg ggcgcgacca accagctgcg cttcgacttt gagtatatga
     5761 acccgatgcc aggcggttcg gtagataact gtattacctt ccagccggtg cagaatcatg
     5821 tggtgattgg tgacgattcc accatcgact tctcgaagta ttaccacttc attccgatgc
     5881 cggatctacg cgcctttgct aacgcgggct tcccattcag ccggatggcg gatctgtcgc
     5941 aaaccatcac cgtgatgccg aaagcgccta acgaagcaca gatggaaacg ttgctgaata
     6001 ctgttggttt tatcggcgca cagacgggct tcccggcgat taatctgacg gtgaccgatg
     6061 atggcagcac cattcagggc aaagatgccg acatcatgat catcggtggt atcccggaca
     6121 aactgaaaga cgataagcag atcgacctat tggtgcaggc gaccgaaagc tgggtgaaaa
     6181 caccgatgcg ccagaccccg ttccccggca ttgtgccgga cgagagcgat cgcgcggcag
     6241 aaacccagtc aacgctgacc tcttccggtg cgatggcggc ggtgattggc ttccagtcgc
     6301 cgtataacga ccagcgcagc gtgattgcgc tgttggcaga tagcccacgc ggttatgaaa
     6361 tgcttaacga tgcggtgaac gatagcggca aacgcgccac catgttcggt tcggtcgcgg
     6421 tgatccgcga gtacggtatc aacagcctac gtgttggcga cgtttattac gtaggtcatc
     6481 tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca tccgattctg ctggcggtgc
     6541 tggcggctat cagtgtgata ttgctggcat gggtactgtg gcgtctgctg cgaattatta
     6601 gtcgtcgtcg tcttaacccg gataacgagt aattgaagat gaatgtgttg cgtagtggaa
     6661 tcgtgacgat gctgttgttg gctgccttta gtgttcaggc agcctgtacc tggcctgcct
     6721 gggagcagtt taaaaaggat tacatcagtc aggaagggcg cgtcatcgac cccagcgacg
     6781 cgcgcaaaat caccacctcc gaagggcaaa gttacggtat gttctttgcc ctggcggcta
     6841 acgaccgtgc agctttcgat aatattctcg actggacgca gaacaatctc gctcagggtt
     6901 ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa agagaacagt aagtgggaag
     6961 tgctggacag caattcggcc tccgatggtg atgtctggat ggcctggtcg ttgctggagg
     7021 cggggcgttt gtggaaagag cagcgttata ccgacatcgg cagcgcgttg ctaaaacgta
     7081 tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc catgttgtta ccgggcaaag
     7141 tgggttttgc tgaggataac agctggcgtt ttaaccccag ctacatgccg ccgacgctgg
     7201 cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct gcgcgaaacc aatcaacatt
     7261 tattgctgga aaccgccccg aaaggctttt cgccagactg ggtgcgctat gagaaagaca
     7321 aaggctggca gctaaaagcc gaaaaaacat tgatcagcag ctacgacgct atccgcgttt
     7381 acatgtgggt aggcatgatg cctgacagcg atccgcaaaa agcgcggatg ctcaaccggt
     7441 ttaaaccgat ggcgacattc actgagaaaa acggttatcc gccggaaaaa gtggatgtgg
     7501 ctacggggaa agcgcagggt aaaggaccgg tcggtttttc tgccgccatg ctgccttttt
     7561 tacaaaaccg cgatgcgcag gcagttcagc gccagcgcgt ggccgataac tttcccggca
     7621 gcgataccta ttacaactat gtgctgaccc tgtttggaca aggctgggat caacaccgtt
     7681 tccgcttctc gacaaaaggt gagttattac ctgactgggg ccaggaatgc gcaaattcac
     7741 actaaacata ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg aggcagcacc
     7801 aaccatcagc agaccctgcg gatggacagc ggcaacacta atgccgtgcg cgggctggcg
     7861 aatatttacc gccagcagtc gccagaaaaa gctgaagcgt ttatcgcctc gctctctgcc
     7921 agccagcggc gcagcattga tgatatcgaa cgcagcctgc aaaacgaccg tctggcacag
     7981 caggcagagg tactggaaaa ccagggcaaa tgggcgcagg cggcagcact tcagcggcaa
     8041 cgactggcgc tggaccccgg cagcgtatgg attacttacc gactttcgca ggatctctgg
     8101 caggccggac aacgcagcca ggccgatacg ttaatgcgca atctggcgca gcagaagccg
     8161 aacaacccgg agcaggttta cgcttacggg ctgtacctct caggtcacaa ccaggacaga
     8221 gcggcgctgg cgcatatcaa cagcctgccg cgcgcgcagt ggaacagcaa tattcaggag
     8281 ctggttaatc gactgcaaag cgatcaggtg ctggaaaccg ctaatcgcct gcgagaaagc
     8341 ggcaaagagg ctgaagcgga agcgatgctg cgtcagcaac caccttccac gcgtattgac
     8401 ctcacgctgg ctgactgggc gtaacaacga cgtgattaca ccgccgcccg cactgcatat
     8461 cagaatgtcc tgacgcggga gccaactaac gccgatgcca ttcttggtct gacggaagtg
     8521 gatattgctg ccggtgacaa agcggcggca cgtagccagc tggcgaaact gcctgccacc
     8581 gataacacct cgctgaacac tcagcggcgc gtggcgctgg cacaggcgca gcttggagat
     8641 accgcagcgg cgcagcagac gtttaataag ttgatcccgc aggcaaaatc tcagccaccg
     8701 tcgatggaaa gcgcgatggt gctgcgcgac ggtgcgaagt ttgaagcgca ggcgggcgat
     8761 ccaaagcagg cgctggaaac ctacaaagac gccatggtcg catccggtgt gactacgacg
     8821 cgtccgcagg ataacgacac ctttacccga ctgacccgta acgacgagaa agatgactgg
     8881 ctgaaacgcg gcgtgcgcag cgatgcggcg gacctctatc gccagcagga tcttaacgtc
     8941 acccttgagc acgattactg gggttcgagc ggcaccggtg gttactccga tctgaaagcg
     9001 cacactacca tgttgcaggt ggatgcgccg tattctgacg ggcggatgtt ctttcgcagt
     9061 gatttcgtca atatgaacgt cggcagtttc tccactaatg ccgatggcaa atgggatgac
     9121 aactggggca cctgtacatt acaggactgt agcggcaacc gcagccagtc ggattccggt
     9181 gccagcgtgg cggtcggctg gcgaaatgac gtttggagct gggatatcgg taccacgccg
     9241 atgggcttca acgtggtgga tgtggtcggc ggcatcagtt acagcgatga tatcgggccg
     9301 ctgggttaca ccgttaacgc ccaccgtcgg cccatctcca gttctttgct ggcctttggt
     9361 gggcaaaaag actccccgag caataccggg aaaaaatggg gtggcgtacg tgccgacggt
     9421 gtggggctaa gtctgagcta cgataaaggt gaagtaaacg gcgtctgggc atcgcttagt
     9481 ggcgaccagt taaccggtaa aaatgtcgaa gataactggc gcgtgcgctg gatgacgggc
     9541 tattactata aggtcattaa ccagaacaat cgccgcgtca caatcggcct gaacaacatg
     9601 atctggcatt acgacaaaga tctgagtggc tactcactcg gtcagggcgg ttactacagt
     9661 ccgcaggaat acctgtcgtt tgccataccg gtgatgtggc gggagcgcac ggaaaactgg
     9721 tcgtgggagc tgggggcgtc tggctcgtgg tcgcattcac gcaccaaaac catgccgcgt
     9781 tatccgctga tgaatctgat cccgaccgac tggcaggaag aagctgcgcg gcaatccaac
     9841 gatggcggca gcagtcaggg cttcggctac acggcgcggg cattacttga acgacgtgtt
     9901 acttccaact ggtttgttgg cacggcaatt gatatccagc aggcgaaaga ttacgcaccc
     9961 agccatttcc tgctctacgt acgttattcc gccgccggat ggcagggtga catggattta
    10021 ccgccgcagc cgctgatacc ttacgccgac tggtaagttt tcagatagcg cctctcttaa
    10081 tgccgctgcg atcgggtata ctcgggcggc aatctgggat ttccgggggg agacaatttg
    10141 cgcgtaagtc gctcgttaac aatcaagcag atggcaatgg tggcagccgt tgtcctggtg
    10201 ttcgttttta ttttttgcac cgttttgctg ttccatctgg tccagcagaa tcgctataac
    10261 acggctacgc aactggaaag cattgctcgc tctgtccgcg aacccttatc ttcagctatt
    10321 ttgaaaggcg atattcccga agcggaagct attcttgcca gcattaaacc ggcaggcgtg
    10381 gtcagccgtg ccgatgtagt gctgcctaac cagttccagg cgctgcgtaa aagttttatt
    10441 ccagagcgtc cggtgccggt aatggttact cgcctgtttg agctaccggt tcaaatctcg
    10501 ctgggcgtct actcgctcga acgtccggca aatccgcagc caatagccta tctggtgcta
    10561 caggcggatt ccttccgtat gtataagttc gtgatgagca ctctctcaac gttagtgacc
    10621 atttacttac ttttgtcgct tatcctgacc gtcgccatca gctggtgcat taaccgcctg
    10681 attttgcatc cgttacgcaa tattgctcgc gaacttaacg ccatcccagc ccaggagctt
    10741 gttggtcacc aactggcatt accgcgtctg catcaggacg atgaaatcgg tatgttggtg
    10801 cgcagttaca acctcaacca gcaattgctg cagcgccatt atgaagaaca gaacgaaaat
    10861 gcgatgcgct tcccggtgtc ggatttgccg aacaaagcct tgctgatgga gatgctggag
    10921 caggttgtcg cgcgtaaaca aaccaccgcg ctgatgatca tcacctgtga aaccctgcgt
    10981 gatactgcgg gcgtgctgaa agaggcgcaa cgagaaattc tgctgctgac gctggtggaa
    11041 aaactcaaat cggtactgtc gccacgtatg atcctcgcgc agattagcgg ttatgacttt
    11101 gctgtcattg ccaacggtgt acaggaaccg tggcacgcaa tcactttggg tcagcaagta
    11161 ctcactatca tgagcgagcg cctgccgatt gaacgtattc aactccgtcc gcactgtagc
    11221 attggcgtgg cgatgttcta cggcgatctc accgccgaac agctttacag tcgcgctatt
    11281 tctgcggcat ttaccgctcg ccataaaggc aagaatcaga ttcagttctt tgatccgcag
    11341 cagatggaag cagcccagaa gcggttgacg gaagagagcg atatccttaa tgcactggaa
    11401 aatcatcagt ttgcaatttg gttacagcca caggtcgaga tgaccagcgg taaactggtc
    11461 agtgcggaag tgttactgcg tatccagcaa ccggatggca gttgggacct gccggatggc
    11521 ttaatcgatc gcattgagtg ctgtgggctg atggttaccg tcggtcactg ggtgctggaa
    11581 gagtcctgtc gattgcttgc agcctggcaa gagcgcggca ttatgctgcc cttgtcggta
    11641 aacctctctg cgctgcaact gatgcacccg aatatggtgg cggatatgct ggaactgtta
    11701 acccgctatc gcattcagcc gggaacactg attctggaag tgacagaaag ccgacgtatt
    11761 gacgaccctc atgctgcggt ggcaatcctc cgtccgctgc gcaatgccgg agttcgggtg
    11821 gcgctggatg atttcggcat gggctacgca gggctgcgtc agctgcagca tatgaaatcg
    11881 ttgccaatcg acgtactgaa aatcgacaaa atgtttgttg aaggcttgcc ggaagatagc
    11941 agcatgattg ctgcaattat catgctggcg cagagcctga acttacaaat gattgccgaa
    12001 ggcgtggaga ctgaagcaca acgagactgg ctggcaaaag cgggcgttgg tattgcccag
    12061 ggcttccttt ttgctcgccc actccctatt gaaatcttcg aagagagtta cctggaagaa
    12121 aagtagctac cccaaactga ttacaaaact ttaaaaagtg ctggtttgtg cgagccagct
    12181 caaacttttt aacctttttg tttcaattat gatccaggta catttctgtg atgttgtctg
    12241 ggtgttattt taaggccgca ggtaccccca taaccttaca agacctgtgg ttttactaaa
    12301 ggacacccta tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata
    12361 gccattggta ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt
    12421 ggcgacggct tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc
    12481 gtaacgggca ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca
    12541 ctgctttact ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac
    12601 gtcgtgcagc ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta
    12661 gcggtttacg ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc
    12721 ccggcgagcg tcattggcgc attttccagc ggtaacattc tgcaggtgct gctgtttgcc
    12781 gtactgtttg gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg
    12841 atcgaaagtt tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct
    12901 attggtgcgt tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg
    12961 caactggggc agctgattat ctgtttctac attacctgta tcctgtttgt ggtgctggta
    13021 ttgggttcaa tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt
    13081 gaagaactgc tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc
    13141 gacaagatgg agaaactcgg ctgccgtaaa tcggtggtgg ggctggtcat cccgacaggc
    13201 tactcgttta accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc
    13261 caggccacta acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg
    13321 ctttcttcta aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg
    13381 ctttctgcgg tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc
    13441 tttatgtcag aagctcgtgc gctaactaac ctggtcggta acggcgtagc gaccattgtc
    13501 gttgctaagt gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt
    13561 gcgccggatg gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc
    13621 cttccccgag tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg
    13681 tgagttcagg tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga
    13741 agtgttttta acgtcattta gactgaccga caacgggcag tttttttatt accaggatag
    13801 ttgatcaggg gttcacatgc agggcataaa aattcgactt ttagcgggcg gtttgctgat
    13861 gatggccact gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca
    13921 ggggacgctt tccaacggtt tacagtggca agtgctgacc accccccagc gtcccagcga
    13981 tcgtgttgaa attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag
    14041 cggttacagt cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc
    14101 acaggcgcgt tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat
    14161 tgtctcttat gacaccacgc tgtttaatct gagtttgccc aataaccgta acgatttgct
    14221 gaaagaagcg ctctcttatc tggcaaatgc cactggcaaa ttgaccatca caccagaaac
    14281 catcaaccac gcgctgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga
    14341 gggctggtgg cgctatcgtc tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc
    14401 gctgaaacaa cccgttgaag cggaaaaaat taaagatttc tatcagaaat ggtacacccc
    14461 ggatgcaatg acgctactgg tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat
    14521 caacaaaacg tttggcgaac tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct
    14581 ttctccgctg cgtgcggaag cggtgagtat tatgactgac gcggtgcgtc aggaccggtt
    14641 atctatcatg tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta
    14701 ctggcgtgcg gacctggccc gcgaggcgct gttctggcat gttcagcaag cgttaagcgc
    14761 cagtgacagc aaagacatcg gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca
    14821 gtgtgccatc aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc
    14881 gcgtgaactg gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt
    14941 ggcgcaaaag aaactggagc tgcagaaact gtttgccgcc tatgcacggg ctgataccga
    15001 tattctgatg ggccagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga
    15061 gcagtatcag aaactgcggc aggatttcct taacagcctg actgtggaga tgttaaatca
    15121 ggatctgcgt cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga
    15181 gccggaattt aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatctac
    15241 cgccgctgcg accacctctg tcgccacgga tgacgtacat cctgaagtga cggatattcc
    15301 acctgcacag taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga
    15361 gctggataga ggggtaacac tttatccagc cttttgcata tgctgcgttt acgctggcat
    15421 cgcctcacgc gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc
    15481 gcgtttcgcc gcgttttccg cactgccgcc cgtcaaacgt accgccagat aaccggcgct
    15541 gaaagagtcg ccagctgcgg tggtatcgat cactttttct ttcggcagtt tcaccgccgg
    15601 aacatcaact aacgcttcac cagcaatgga caccaggcaa gaatccgccc cgcgtttcac
    15661 caccacttct ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg
    15721 accccacagc gcgtcttcgt cgtccagcgt caggaaggcg atatccgtgc attccagcat
    15781 ttgctggtac acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt
    15841 gtcgaaaatc acttttccgc ctttggcgcg gcattcgcgc agcagggaaa gcagcttttc
    15901 gcggctggtc gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt
    15961 cgccagctct tcgcaaatcg ccgcagactg ctcgctcgcc agccagaatt tggcggcggc
    16021 ttcgttacgc cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag
    16081 gcccggaaga cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgtgcca
    16141 ggcgtccagc atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt
    16201 taatgccgca ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc
    16261 gaaaccgcgc ttaacgtccg cgtctttctc ggaaagctca atcatgcatt cgccaatcac
    16321 ggcaatcttt ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc
    16381 tgtggtggtt tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa
    16441 cgtattcctg aaagattccg ttgtggagtg aggaaattta ccccggccag gcggtaaagt
    16501 tctgccctta cgcgccgata atctttgtcg agtccgggca gcatcacttt taaacacagg
    16561 acatctttga tgataaggca ggttatccag cgaataagca accctgaagc aagcatcgag
    16621 agcttgcagg aacggcgttt ttggttgcag tgtgagcgtg cttacacctg gcagccgatc
    16681 tatcaaacat gcgggcggtt aatggccgtg gagctattaa cggtggtcac gcatcccttg
    16741 aacccttcgc aacgcctgcc gccggatcgc tattttactg aaatcaccgt cagccatcgg
    16801 atggaggttg tgaaagagca gattgatttg ctggcgcaaa aagccgactt ctttgtagag
    16861 cacggcctgc tggcatcggt caatattgat ggccctacgc tcatcgccct gcgtcagcaa
    16921 ccaaaaatcc tgcgccagat tgagcgtctt ccctggctgc gtttcgaact ggtggagcat
    16981 atccgtctgc cgaaagattc aacctttgcc tcgatgtgtg aatttggccc gctgtggctg
    17041 gatgattttg gtaccgggat ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac
    17101 atcaaaatcg cgcgagaact gtttgtgatg ctgcgtcagt cgccggaagg ccgcacactc
    17161 ttttctcagc ttttacatct aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta
    17221 gaaacaccgg aagagtggcg tgatgttcag aactcgcccg cattcgccgc acaaggctgg
    17281 tttctttcac gcccggcacc gatggaaacg ctgaatacgg cggttctggc gctataagct
    17341 gcctcatttt ccgccgggct ggactatctt taggactggc acaggaagaa tatgaggaag
    17401 cgaacgatga gcaaggcagg caaaataacc gctgcgattt caggggtttt cttgttgttg
    17461 attgtcgtgg cgatcatttt gattgcaact tttgactgga atcgactcaa accgaccatc
    17521 aaccagaaag tctctgcgga gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg
    17581 gtgtgggagc ggcaaaaaca agaaactggc tggcgcagct gggtgccgtg gcctcatgta
    17641 catgcagaag acatcattct tggtaatcca ccggatattc ccgaagtcac gatggtgcat
    17701 ttgccgcgag tagaggcaac gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg
    17761 ccgtggatca agctcgaaaa gcccgacgcg cgcctgattc gcctctctga aaagaacaat
    17821 aactggacgt ttaatcttgc caacgatgac aacaaagacg cgaatgcaaa gccgtcggca
    17881 tggtcgtttc ggctggataa tattcttttc gatcaagggc ggatcgccat tgatgacaaa
    17941 gtaagcaaag cggatctgga aatttttgtt gatccgttag gcaagccgct gccattcagc
    18001 gaagttactg gatcgaaagg taaagcggat aaagaaaagg tgggcgatta catttttggc
    18061 ctgaaggcgc agggacgtta taacggtgaa ccgctcacgg gtacgggaaa aataggcggt
    18121 atgctggcgc tgcgtggcga agggacgccg tttccggtac aggctgattt ccgttcaggt
    18181 aatacccgtg ttgcttttga tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat
    18241 ttacggctta aattttctgg cgattcactg ggtgatctct atgaactgac gggcgttctg
    18301 ctgcccgata ccccgccgtt tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa
    18361 aaatcgtcgg tctttgatta tcgcggtttt aatgggcgaa ttggcgatag cgatatccac
    18421 ggttctctgg tctacaccac cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg
    18481 cggcaattgc ggctggcgga cctgggaccg ttgattggcg ttgattccgg gaaaggggca
    18541 gaaaagtcga aacggtctga acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa
    18601 gtgctgcctt atgaccgctt cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc
    18661 ttcaaagggc ggcgcattga gcatggcagt agcctgccga ttagcgatct ttctactcat
    18721 atcatcctca aaaatgctga cctgcgcctg caaccgctga aatttggcat ggcgggcggc
    18781 agcattgcgg cgaatattca tctggaaggc gataaaaagc cgatgcaggg gcgggcagat
    18841 attcaggctc gtcgactgaa actgaaagaa ctgatgcccg atgtggaact gatgcagaag
    18901 acgctggggg aaatgaacgg tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg
    18961 cttttaggca acagtaacgg caacctgaaa ctgttgatga atgacgggct ggtgagccgc
    19021 aacctgatgg agattgttgg gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt
    19081 gacgatgagg tgcgggtgaa ctgcgcggcg gcgaatctgc atattgccaa cggcgtggcg
    19141 cgcccgcaga tttttgcttt cgatactgag aacgcgttga ttaatgttac cggcacggca
    19201 agttttgctt cggaacagct ggatttgact attgatccgg agagtaaagg gattcggatt
    19261 atcacactgc gttcgccgct gtatgtgcgt gggacgttta aaaatccgca ggctggggtg
    19321 aaagccggac cgctgattgc ccgtggtgct gttgctgcgg cactggcaac gctggtaaca
    19381 ccggcggcgg cgttactggc actgatctca ccttccgaag gggaggctaa tcagtgtcgg
    19441 acgatattgt cgcagatgaa gaagtgatga taattgccgg atgtctgaca tccggcaatt
    19501 aaacagattt agatttttgg caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc
    19561 ttatccggcc tacaaatcct tgtgaattca atatattgta tggaaattca gtcctgataa
    19621 gcgtagcgca tcaggctttt cacacttatt acaacgactg atgtcgcgtc tcatgggtca
    19681 gcagcagggc gattaacgtc aagccagcca tcgccgccag atataacccc accgcaccta
    19741 acccgtagtt agcctgcaac caagctgcga tatacggcgc aacggaagcc ccgagaatcg
    19801 acgatacgtt gtaagagaac gatgctccgg tgtaacgcac ttctgtcgga aacagctctg
    19861 gtaacagcgc acccatcggc ccgaaggtca gccccatcag acttaacccc agcagcagga
    19921 aggcaaaaac cagaatcggg ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga
    19981 acaggatgat cagcgtggtg atgatcacca tgcttttacg gcgaccaaag gcatcagcca
    20041 gtaatccggc gactggcacc atcacgccaa aaccaataac tgccatcatc agcatccaca
    20101 acacttcgtt acgcggcagg ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag
    20161 agtagaccgt catgatgtaa aacagcgtat aggttgccag cataatgaac gtacccagta
    20221 cggttacgcg aacatgtttg gtcagcagcg tacccagcgg gatcttcacc tgttttttcg
    20281 ctttagcgac tttctcaaac accggcgact catgcagcga cacgcgaaca tacaggccga
    20341 taatgaccag caccgccgag aagataaatg gcacacgcca gccccagctc ataaactgct
    20401 catcggtcag tagccaggaa agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg
    20461 gtgcgcctag ctgtgggaag gagccataca gtgcacgttt gcgcggtggg gcgttttcag
    20521 tcgccagcag cgccgcgccg ccccattcac cgcctaagcc cagaccctga ccaaatcgag
    20581 ccagcgccag cagcagtggg gcgaaaatac caatcgtggc atagcccggc agcagaccaa
    20641 tcaccacggt cgaaatcccc atcgttagca acgaggcgac cagcgtcgct ttacgcccaa
    20701 cgcgatcgcc aaaatgacca aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg
    20761 cgaaggtggc gagcgactgt agcgttgctg ccgttggatc gccctgcggg aagaagatat
    20821 gcggaaacac aataacggcc gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg
    20881 tgccaatgag agaggcgaca aggactttat tacgcgagtt gatcggcgtg tattcttgtt
    20941 cgtggtcgag tgttgtggct gttgcttgca taatcttttc ttattttttg gctaacgaat
    21001 agccatatta cgcacagcaa aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca
    21061 aaaaccaggc aaaaagggga taatgtttag gccagtgatt tttcttctat gaaatgcttc
    21121 acataatttt ataaatagtg gataaaactg gtttcaggtt aaataaaagt taccaactgg
    21181 atttatatgc tggggttatg agttggagtg aaattgtttg ctcccaaata gatggctgaa
    21241 gaggctctgg ggaagagttt caagaggtta tgtgcgcatt atagccggat gcgatgctgg
    21301 cgcatcttat ccggcctacg gggagccagg ccgaataaaa tcgtatgccg gataaggcgt
    21361 ttacgccgca tccggcctat tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt
    21421 atattcggcg gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga agaagtagaa
    21481 aaatgccatc agccccagca cggaaccaaa tgctgcgcca gagggggatt tcatcagcga
    21541 tggcagggtg taggtcatca cgattttaat cacttcaaaa ccaatagcag ccagaaatgt
    21601 tccgcgaatc agggcttttt tgcgcggacg gtggcgcggc aaccgccaga agatccagaa
    21661 gaaaagcaga tagttggcga agatggaaat cgccaggcca atcaatcgcc acgtcggttt
    21721 cagccactca atgctgttca ggtgcagggc gctaataatc atttgctgcg ccgaaccggc
    21781 aaccgaggtg atcgaaagcg tcacaatcag cgcaatcaac aaaccaatca gtgaaataaa
    21841 atcacgcaga tattttaccc agaacttctc ctgatcttgc ggcgagcgtt cccagacatc
    21901 gcgcgactgg gcacgaatcg cttcacgcag gttacccatc cagttgatgc cggaataaag
    21961 tgccaccgcc aggccgacaa gccctacagt cgtacgctgc tgaacggcgg tgttgatggt
    22021 gtttttcaac gtggcggcta gtgtcggatc gctgatgttt tgcagaattt tgtcgaagat
    22081 atcctgtagc aacatcggat gggaggccag cacaaagccc cctgcggcaa acgacaccat
    22141 caaaatcggg atcatcgaca aaaatgagaa ataggtgata gccgcaccaa actggttacc
    22201 cagccgatcg ttaaagcgtt ctgtcgcgcg aataaggtgc gcaataaccg gttggcgttg
    22261 gactttttcg gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc cgcctttctc
    22321 gctattatcc aactgcttaa tcggctcgtg ctccagatcc tgggtgggac gtttgatctc
    22381 gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa aaagtatagt
    22441 cggtcagtcc aggtgctctt tcattacgcc agccagccac tgcataaaca ggtttacacg
    22501 ccgggaaagc tcccgacgct gcgggtaaac cagcgaaagg gagagcggct cggcacggta
    22561 gcctggcaac acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg
    22621 gatctgaata atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac
    22681 cgtcaacatg ccgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc
    22741 taacggatgt acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc
    22801 ggggctttgc ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt
    22861 tagtttgccg aggggacggg cgataacgcc atcttccggt aacgcgccag tgcgtatcac
    22921 gcaatcaaaa ccatcatgaa gaatatctac cggacggtta tggctactca gttccagctc
    22981 aattcccgga tgctgataga gaaattccga cagacgcggc agtaacaggt ttttcgcgat
    23041 tcctgacggg atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag
    23101 accgtccagt tcgctgagat tactcaacac atcttttgcc cgttgataat aggtcatgcc
    23161 ttctggcgtg agtttgaccc gtcgcgtagt gcgctggaga agctgggtgc caagttgatg
    23221 ttccagtgcc tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc
    23281 gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc
    23341 catttctttg accgattgtt gttcacagcg aaacagtaac gcgcatatcg cattgtttat
    23401 tgttttttct aacatctaat gccgggtttc acaatgttgt catgacgaat gtggcgattt
    23461 ctcagcagga aaagggcgga atagctttat gagaaaggtg aatcccctta aacagagcgt
    23521 atttttcagt gattcccttt aaagtgaata gaggacgtat tggctatttg tgcttatttt
    23581 tgtctcattg ccatttgtta ttttggcttg tagtcgcgga atttcatttt taattgcatg
    23641 cgtactaacc accaatataa ggaaatcctc aaaattgttt taaatagtgc cctcgacatg
    23701 taatttcagg cgtttagctg taactattta tatcggtaac tattttctac gcgtcatctc
    23761 tgtagaatgg ccaccgccaa atggcgaatt cttatttttt ctcgcgggta atacccttcc
    23821 actacttaag tctttttaac gtattcaggt ggagattcag ttatgcaaat agtcatgttt
    23881 gacaggcagt caatatttat tcatggaatg aaaatcagtt tacagcagcg tattccagga
    23941 gtgagtattc agggggtcag tcaggcagac gagttatggc aaaagctgga aagttaccct
    24001 gaagccttag ttatgctcga tggcgatcag gatggtgagt tttgctactg gttgctgcaa
    24061 aaaaccgtgg tgcagtttcc tgaggttaag gtgttaatca cggcgacgga ttgcaataaa
    24121 cggtggttac aggaagttat ccattttaat gtgctggcca ttgtgcctcg cgattcaacc
    24181 gtcgagacat ttgcgttggc ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga
    24241 gactggcgga cgacgccgga aaaggacatt aaagatctca aatcgttaag cgcccgccag
    24301 cgggagattt taaccatgtt agcagcgggc gaatc
//
LOCUS       DAJTRQ010000051        24112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000051 DAJTRQ010000000
VERSION     DAJTRQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1270
                     /gene="glpK"
                     /locus_tag="OO332_002119"
     CDS             <1..1270
                     /gene="glpK"
                     /locus_tag="OO332_002119"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5942597.1"
                     /translation="TNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDY
                     ISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT
                     DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPI
                     SGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGE
                     VNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPY
                     WDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAV
                     ANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFR
                     PGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1500..2510
                     /gene="glpX"
                     /locus_tag="OO332_002120"
     CDS             1500..2510
                     /gene="glpX"
                     /locus_tag="OO332_002120"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5942598.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2607..3353
                     /gene="fpr"
                     /locus_tag="OO332_002121"
     CDS             2607..3353
                     /gene="fpr"
                     /locus_tag="OO332_002121"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5942599.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="OO332_002122"
     CDS             complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="OO332_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5942600.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3813..4112)
                     /locus_tag="OO332_002123"
     CDS             complement(3813..4112)
                     /locus_tag="OO332_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5942601.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4324..4764)
                     /locus_tag="OO332_002124"
                     /pseudo
     CDS             complement(4324..4764)
                     /locus_tag="OO332_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4865..5464
                     /locus_tag="OO332_002125"
     CDS             4865..5464
                     /locus_tag="OO332_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5942602.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5572..6339
                     /gene="tpiA"
                     /locus_tag="OO332_002126"
     CDS             5572..6339
                     /gene="tpiA"
                     /locus_tag="OO332_002126"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5942603.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="OO332_002127"
     CDS             complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="OO332_002127"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5942604.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7256..8245)
                     /gene="sbp"
                     /locus_tag="OO332_002128"
     CDS             complement(7256..8245)
                     /gene="sbp"
                     /locus_tag="OO332_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5942605.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="OO332_002129"
     CDS             complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="OO332_002129"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5942606.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="OO332_002130"
     CDS             complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="OO332_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5942607.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="OO332_002131"
     CDS             complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="OO332_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5942608.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11408..12106
                     /gene="cpxR"
                     /locus_tag="OO332_002132"
     CDS             11408..12106
                     /gene="cpxR"
                     /locus_tag="OO332_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5942609.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12103..13476
                     /gene="cpxA"
                     /locus_tag="OO332_002133"
     CDS             12103..13476
                     /gene="cpxA"
                     /locus_tag="OO332_002133"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5942610.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="OO332_002134"
     CDS             complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="OO332_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5942611.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="OO332_002135"
     CDS             complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="OO332_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5942612.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="OO332_002136"
     CDS             complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="OO332_002136"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5942613.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16553..17587
                     /gene="rhaT"
                     /locus_tag="OO332_002137"
                     /pseudo
     CDS             16553..17587
                     /gene="rhaT"
                     /locus_tag="OO332_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17584..18450)
                     /gene="rhaR"
                     /locus_tag="OO332_002138"
     CDS             complement(17584..18450)
                     /gene="rhaR"
                     /locus_tag="OO332_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5942614.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="OO332_002139"
     CDS             complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="OO332_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5942615.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19630..21099
                     /gene="rhaB"
                     /locus_tag="OO332_002140"
     CDS             19630..21099
                     /gene="rhaB"
                     /locus_tag="OO332_002140"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5942616.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21096..22355
                     /gene="rhaA"
                     /locus_tag="OO332_002141"
     CDS             21096..22355
                     /gene="rhaA"
                     /locus_tag="OO332_002141"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5942617.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22597..23421
                     /gene="rhaD"
                     /locus_tag="OO332_002142"
                     /pseudo
     CDS             22597..23421
                     /gene="rhaD"
                     /locus_tag="OO332_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            23431..23745
                     /gene="rhaM"
                     /locus_tag="OO332_002143"
     CDS             23431..23745
                     /gene="rhaM"
                     /locus_tag="OO332_002143"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5942618.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24046..>24112
                     /locus_tag="OO332_002144"
     CDS             24046..>24112
                     /locus_tag="OO332_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5942619.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT         5986 a         6295 c         6130 g         5701 t
ORIGIN      
        1 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
       61 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
      121 tttagaagat tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac
      181 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
      241 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
      301 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
      361 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
      421 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
      481 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
      541 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
      601 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
      661 ctatgcgttg gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga
      721 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
      781 caatggtgtg tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta
      841 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
      901 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
      961 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
     1021 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
     1081 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
     1141 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
     1201 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
     1261 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
     1321 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
     1381 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
     1441 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
     1501 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
     1561 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
     1621 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
     1681 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
     1741 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
     1801 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
     1861 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
     1921 ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
     1981 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
     2041 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
     2101 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
     2161 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
     2221 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
     2281 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
     2341 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
     2401 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
     2461 tccactatct gaatcgcaaa gacccggaaa tgcaagtgca cattctctga gtgatttgat
     2521 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
     2581 caagtcaatc aaaacaggag aaaaaaatgg ctgattgggt aacaggcaaa gtcactaaag
     2641 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
     2701 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
     2761 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
     2821 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
     2881 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
     2941 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
     3001 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
     3061 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
     3121 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
     3181 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
     3241 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
     3301 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
     3361 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
     3421 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
     3481 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
     3541 tgtcttcgga cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctc
     3601 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
     3661 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
     3721 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
     3781 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
     3841 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
     3901 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
     3961 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
     4021 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
     4081 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
     4141 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
     4201 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
     4261 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
     4321 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
     4381 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
     4441 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
     4501 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
     4561 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
     4621 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
     4681 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
     4741 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
     4801 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
     4861 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
     4921 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
     4981 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
     5041 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
     5101 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
     5161 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
     5221 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
     5281 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
     5341 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
     5401 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
     5461 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
     5521 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
     5581 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
     5641 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
     5701 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
     5761 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
     5821 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
     5881 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
     5941 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
     6001 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
     6061 atcgcttatg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
     6121 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
     6181 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
     6241 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
     6301 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
     6361 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
     6421 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
     6481 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
     6541 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
     6601 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
     6661 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
     6721 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
     6781 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
     6841 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
     6901 acgatacgtt gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
     6961 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
     7021 caaacattcc tcaaggacac tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
     7081 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
     7141 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
     7201 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
     7261 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
     7321 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
     7381 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
     7441 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
     7501 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
     7561 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
     7621 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
     7681 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
     7741 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
     7801 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
     7861 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
     7921 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
     7981 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
     8041 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
     8101 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
     8161 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
     8221 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
     8281 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
     8341 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
     8401 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
     8461 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
     8521 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
     8581 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
     8641 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
     8701 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
     8761 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
     8821 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
     8881 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
     8941 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
     9001 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
     9061 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
     9121 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
     9181 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
     9241 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
     9301 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
     9361 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
     9421 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
     9481 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
     9541 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
     9601 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
     9661 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
     9721 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
     9781 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
     9841 aaaggcagag agtcttccat ttccaaatgg atctgaataa agcgggtcgg ccctgactgc
     9901 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
     9961 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    10021 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    10081 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    10141 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    10201 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    10261 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    10321 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    10381 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    10441 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    10501 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    10561 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    10621 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    10681 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    10741 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    10801 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    10861 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    10921 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    10981 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    11041 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    11101 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    11161 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    11221 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    11281 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    11341 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    11401 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    11461 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    11521 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    11581 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    11641 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    11701 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    11761 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    11821 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    11881 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    11941 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    12001 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    12061 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    12121 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    12181 ttacccaagc tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg
    12241 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    12301 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    12361 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    12421 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    12481 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    12541 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    12601 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    12661 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    12721 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    12781 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    12841 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    12901 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    12961 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    13021 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    13081 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    13141 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    13201 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    13261 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    13321 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcaat tgttgaaacc
    13381 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    13441 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    13501 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    13561 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    13621 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    13681 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    13741 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    13801 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    13861 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    13921 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    13981 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    14041 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    14101 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    14161 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    14221 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    14281 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    14341 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    14401 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    14461 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    14521 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    14581 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    14641 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac
    14701 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    14761 gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt
    14821 cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    14881 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    14941 cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca
    15001 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg
    15061 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    15121 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    15181 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    15241 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    15301 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    15361 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    15421 gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    15481 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat
    15541 tgtttttatt ttggaattgt tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    15601 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    15661 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc
    15721 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    15781 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    15841 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    15901 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    15961 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    16021 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    16081 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    16141 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    16201 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    16261 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    16321 ggtttattat agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa
    16381 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    16441 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    16501 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    16561 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    16621 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    16681 tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc
    16741 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    16801 ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat
    16861 tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    16921 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt
    16981 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    17041 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    17101 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    17161 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    17221 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    17281 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    17341 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat
    17401 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    17461 cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt
    17521 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    17581 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    17641 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    17701 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag
    17761 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    17821 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    17881 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    17941 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    18001 agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    18061 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    18121 aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    18181 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    18241 atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca
    18301 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    18361 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    18421 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    18481 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat
    18541 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    18601 atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg
    18661 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa
    18721 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    18781 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    18841 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga
    18901 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    18961 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    19021 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    19081 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    19141 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat
    19201 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    19261 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat
    19321 ccacactacg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    19381 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    19441 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag
    19501 caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    19561 agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    19621 gatcacatta tgacctttcg caattgtgtc gccgtagatc tcggcgcatc cagtgggcgc
    19681 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    19741 tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa
    19801 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    19861 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    19921 ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa
    19981 ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt
    20041 tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct
    20101 ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc
    20161 aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    20221 gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt
    20281 gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    20341 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct
    20401 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    20461 gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    20521 atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg
    20581 cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    20641 cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg
    20701 caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg
    20761 ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg
    20821 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc
    20881 ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag
    20941 ttaattacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    21001 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    21061 attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    21121 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    21181 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    21241 aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag
    21301 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc
    21361 cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc
    21421 agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    21481 tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc
    21541 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    21601 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    21661 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    21721 atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    21781 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    21841 ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga
    21901 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    21961 gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg
    22021 ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg
    22081 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    22141 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    22201 acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg
    22261 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    22321 cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacaagcgc
    22381 cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc
    22441 ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg
    22501 gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt
    22561 ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    22621 tttgtccagg gaatgatcaa agccaccacc gatgcctagc tgaaaggctg ggacgagcgc
    22681 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac
    22741 ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg
    22801 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct
    22861 aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta
    22921 accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc
    22981 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    23041 gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    23101 agcaccgaat gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc
    23161 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    23221 tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc
    23281 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    23341 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    23401 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta
    23461 aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa
    23521 gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat
    23581 ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    23641 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac
    23701 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg
    23761 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    23821 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    23881 ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc
    23941 tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc
    24001 tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc agctcttact
    24061 gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DAJTRQ010000052        23749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000052 DAJTRQ010000000
VERSION     DAJTRQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..160
                     /locus_tag="OO332_002145"
     CDS             <1..160
                     /locus_tag="OO332_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5942620.1"
                     /translation="NFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKAL
                     NELEHQTK"
     gene            213..1943
                     /locus_tag="OO332_002146"
     CDS             213..1943
                     /locus_tag="OO332_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942621.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2003..2494)
                     /gene="yajQ"
                     /locus_tag="OO332_002147"
     CDS             complement(2003..2494)
                     /gene="yajQ"
                     /locus_tag="OO332_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5942622.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2662..3573
                     /gene="panE"
                     /locus_tag="OO332_002148"
     CDS             2662..3573
                     /gene="panE"
                     /locus_tag="OO332_002148"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5942623.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3536..4126
                     /gene="yajL"
                     /locus_tag="OO332_002149"
     CDS             3536..4126
                     /gene="yajL"
                     /locus_tag="OO332_002149"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5942624.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4180..5628)
                     /gene="thiI"
                     /locus_tag="OO332_002150"
     CDS             complement(4180..5628)
                     /gene="thiI"
                     /locus_tag="OO332_002150"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5942625.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5834..6076
                     /gene="xseB"
                     /locus_tag="OO332_002151"
     CDS             5834..6076
                     /gene="xseB"
                     /locus_tag="OO332_002151"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5942626.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6076..6975
                     /gene="ispA"
                     /locus_tag="OO332_002152"
     CDS             6076..6975
                     /gene="ispA"
                     /locus_tag="OO332_002152"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5942627.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7000..8862
                     /gene="dxs"
                     /locus_tag="OO332_002153"
     CDS             7000..8862
                     /gene="dxs"
                     /locus_tag="OO332_002153"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5942628.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8917..9891
                     /gene="yajO"
                     /locus_tag="OO332_002154"
     CDS             8917..9891
                     /gene="yajO"
                     /locus_tag="OO332_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5942629.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9945..10463)
                     /gene="pgpA"
                     /locus_tag="OO332_002155"
     CDS             complement(9945..10463)
                     /gene="pgpA"
                     /locus_tag="OO332_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5942630.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10441..11418)
                     /gene="thiL"
                     /locus_tag="OO332_002156"
     CDS             complement(10441..11418)
                     /gene="thiL"
                     /locus_tag="OO332_002156"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5942631.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11496..11915)
                     /gene="nusB"
                     /locus_tag="OO332_002157"
     CDS             complement(11496..11915)
                     /gene="nusB"
                     /locus_tag="OO332_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5942632.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11935..12405)
                     /gene="ribE"
                     /locus_tag="OO332_002158"
     CDS             complement(11935..12405)
                     /gene="ribE"
                     /locus_tag="OO332_002158"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5942633.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12494..13597)
                     /gene="ribD"
                     /locus_tag="OO332_002159"
     CDS             complement(12494..13597)
                     /gene="ribD"
                     /locus_tag="OO332_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5942634.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13601..14050)
                     /gene="nrdR"
                     /locus_tag="OO332_002160"
     CDS             complement(13601..14050)
                     /gene="nrdR"
                     /locus_tag="OO332_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5942635.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14201..14740
                     /locus_tag="OO332_002161"
     CDS             14201..14740
                     /locus_tag="OO332_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5942636.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15039..15923
                     /gene="tsx"
                     /locus_tag="OO332_002162"
     CDS             15039..15923
                     /gene="tsx"
                     /locus_tag="OO332_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5942637.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16100..16447)
                     /locus_tag="OO332_002163"
     CDS             complement(16100..16447)
                     /locus_tag="OO332_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5942638.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16576..17547)
                     /gene="secF"
                     /locus_tag="OO332_002164"
     CDS             complement(16576..17547)
                     /gene="secF"
                     /locus_tag="OO332_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5942639.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17558..19405)
                     /gene="secD"
                     /locus_tag="OO332_002165"
     CDS             complement(17558..19405)
                     /gene="secD"
                     /locus_tag="OO332_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5942640.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19433..19765)
                     /gene="yajC"
                     /locus_tag="OO332_002166"
     CDS             complement(19433..19765)
                     /gene="yajC"
                     /locus_tag="OO332_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5942641.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19788..20915)
                     /gene="tgt"
                     /locus_tag="OO332_002167"
     CDS             complement(19788..20915)
                     /gene="tgt"
                     /locus_tag="OO332_002167"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5942642.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20970..22040)
                     /gene="queA"
                     /locus_tag="OO332_002168"
     CDS             complement(20970..22040)
                     /gene="queA"
                     /locus_tag="OO332_002168"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5942643.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22133..22706
                     /gene="acpH"
                     /locus_tag="OO332_002169"
                     /pseudo
     CDS             22133..22706
                     /gene="acpH"
                     /locus_tag="OO332_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22711..>23749)
                     /gene="malZ"
                     /locus_tag="OO332_002170"
     CDS             complement(22711..>23749)
                     /gene="malZ"
                     /locus_tag="OO332_002170"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5942644.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         6020 a         6232 c         5841 g         5656 t
ORIGIN      
        1 taattttgcc gcacatttct atctgggaag gatgtatcag aatggtgacg gcgttgagag
       61 aaatatggaa aaagccaggt ttcatctatc taatgcggca gaaggtggaa tagaacctgc
      121 gcttaaagca ttaaatgaac ttgaacatca aacaaaataa aacgctcagg gaccacgtaa
      181 ggttcatcaa acagaaaagg atatagtttg cgatgaaagc gaaatctact ttgttattaa
      241 tgattttatc tgttttttat tctcatcagg ttttatccag cgaccccact cagccggatg
      301 tttattctgt cgtcgagaaa aaactaaact ctgctttacc tctggaagaa aataccacct
      361 tcagatcgca ggctgaatgg tttaatttgc aatatgaatt attagtatct ggctaccctg
      421 aacgtgctta caatttactg agcaaacttg agacagaatc gaaaaacgct aaatattatc
      481 tggatttaac tgcctccaga gtcgctgaag aagaagcacc agagacagcc ttaacttttt
      541 tatcaaaaat tgggttaaat aaaccggcta ctcttacatc ctttgagagc tatatcaata
      601 gctggatgaa aagcaaacaa cccgaaaagg ctatgcagct tctcagcctc gatgagaatg
      661 ccctgaatta ttatttaccg actgtgttag tcgcatatca gatcgttcct gataaggcaa
      721 taagtattta taattctgta tatggagata atgttgttat accctatcag caagtcacca
      781 tgttacttac agttgcggag ggttatcaaa caaaaggcga tataaaaaac gccattcatt
      841 atgccgataa ggcgctgaat atgtttgata aagctattgc tgtagatagc agtcgtgaat
      901 cccattatag tgatgagtat ttgaaactca tgaatatcta cgccgccaat ggcaacaaag
      961 aaaaagcggt ggttttatct caacgtctac acaaagccat taccgaatct caatcgaatt
     1021 acttaagtac tctggaagga attttattgt tttatagaaa taatgatatg cagcaagact
     1081 atcaaaacag cctttcaaat tacatagtat ttatcgatga aatttttgcc ttttccccat
     1141 caacaagaag tgaattaagc ctcattaatt tattaaattc attaaatgag gtggaattga
     1201 tgagaaaacg tctggcgttt ctgatgacag caccagaata cgcttgttat gatagtcagt
     1261 attgttatga agacaaagtc agcgcattaa aaatcctgta tcgtcatcat gacaatgagc
     1321 taatggacaa atatttcacg actcttctta aagagatcag taatcaacca ttagaagact
     1381 gggatataat aataagtaat actggtgaaa aatttagtga agtgggtcta accaaatatg
     1441 ctcaacatct cgcatctttc gctgaagaat tttatataaa agagcaaaaa tcacattctg
     1501 aaagtgaaat gagaggacta ttttcatcac ttgccgaatt atatagtttt ggtaatgata
     1561 ctgccagcgc aaaccgagtg ttccatcagc atgtccctgc cttcaataca gatcatatga
     1621 ttcaatattt tataaatgcc aaagaatgga acaatgccag ggaattattg ataaaagagg
     1681 aaatgttggg tatgcataat ctgatattgt tggagaatat ttgtatgcaa aaaaatgcag
     1741 aatgcatggc acacattacc tttacgctta ataaattaac aacacaacca gcaataacaa
     1801 aaatagatag cgtgggtaat gagcaacttt atcagattgg caaaatctac cataagttgg
     1861 aaattaaacc tgagccagag caacaggttt taattcagtc gctttatgac cgggcatcag
     1921 gaagcgcctc tgcaactcaa taaatgcatg gtcaaaaggg ccaccacggt ggccctgaca
     1981 atcagtagct atatccagaa agttaatcgc ggaagttttt gaactggaac ggctgaccga
     2041 gatcgccacc gcgcaccatc gccatgacag cctgcaaatc atcacgagat ttgccagtca
     2101 cgcggatctc atcgccctga atttgcgcct gcactttcag tttgctgtct ttgatcatct
     2161 tgacgatttt tttctgagtg gcgctctcaa tgccctgttt cagtttcgct tccacaaacc
     2221 aggttttacc gctatgaacg atattttccg gtacatccag tgaactgcct tcaatgccgc
     2281 gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaag tcggactcgc
     2341 tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca acgttacgga
     2401 agtcaaaacg ggactccact tcgcgactcg cgttatcgac cgcgttacgt gcttcctgaa
     2461 gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc atttttgatg
     2521 cgaagcataa tacctgcaaa gttaaataac caacagtcct gcaccaggac gtatccctta
     2581 attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa cacccggagt
     2641 ggttgtgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc cttagggcaa
     2701 ttatggctta cagcactttg taaacagggt catgaagttc agggctggct gcgcgtaccg
     2761 caaccttatt gtagtgtgaa tctggttgag acagatggtt cgatatttaa cgaatcgctg
     2821 accgccaacg atcccgattt tctcgccacc agcgatctgc tcctggtgac gctgaaagca
     2881 tggcaggttt ccgatgccgt caaaagcctc gcatccacac tgcctgtaac tacgccaata
     2941 ctgttaattc acaacggcat gggcaccatc gaagaattgc aaaacattca gcagccatta
     3001 ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat tcatgtggca
     3061 aacggaatca cgcatatcgg tccggcacgg caacaggatg gcgattacag ttatctggcg
     3121 gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg cgccgagctg
     3181 tggcgcaagc tggccgtcaa ctgcgtgatt aatccactga ctgccatctg gaattgcccg
     3241 aacggtgaat tacgtcatca tccgcaagaa attatgcaaa tatgcgaaga agtcgcggca
     3301 gtgatcgaac gcgaagggca tcatacttca gcggaagatt tgcgtgatta cgtgatgcag
     3361 gtgattgatg ccacagcgga aaatatctcg tcgatgttgc aggatatccg cgcgctgcgc
     3421 cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc gcatgggatt
     3481 gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag tgaatatgag
     3541 cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg tcaccacgat
     3601 cgacctcttg gttcgcggcg gtatcaaagt caccactgcc agcgtcgaca gcgatggcaa
     3661 cctggcaatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc tggtcgaagt
     3721 ggctgatggc gaatatgacg tgatcgtgct gcctggtggc attaaaggca cggagtgttt
     3781 tcgcgatagc actctgctgg ttgaaaccgt taaacagttc caccgttccg ggcgtatcgt
     3841 cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct ttccgattgg
     3901 caatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat ggcaggacaa
     3961 gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg gtacagctat
     4021 cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc atgaagtggc
     4081 atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc ggatgcggca
     4141 aacgtcgcat ctgaccagat gcgacgtaaa aactacaaat tacgggcgat ataccttcac
     4201 attgttaaag ccttgctcgc gcagatagag cgcctgcaga cggctcatca ccccgcgctc
     4261 acaccacagc agccaggttt tgttctggtc gagatcgcca aatttggtgc tcagtttata
     4321 gaacggcaga gaaaccacat caatcccttc gactttcagt ggcttatctt cctgttcatc
     4381 gacagaacgg atatcgagaa tcacgtcgtt cgggccgaag ccgttgacgg tttccacttc
     4441 caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat tcgcttcctc
     4501 aaccacttta tcgagaatac tgaagtcgaa cttctcttct tccgcttcaa tcttcgattt
     4561 aaccgctttc accgtcgggc ttttggagat cacgccacaa tattccggca tcgtgcgagc
     4621 aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt cgtaagaaat
     4681 cagcggacgc aggatcagcg tgtcagagac gttatcaatc aggcgcaggt tggtcagcgt
     4741 ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc cgtaacgttc
     4801 agccacttta gatgcggcac gcaccatcat acgtttgagg ataacgccca tctgaccgtc
     4861 gtcgattttc tcgagaattt ccccgacaac cggttcgaaa ttaatggcga caaaacgcac
     4921 gcggtgggag ctgccaaagc ggttccacag ataatgcgcc acctgacgca cgccaatttc
     4981 atgcgctgcg ccgccgaggt taaagaagca gtaatgcacg cggcagccgc gacgcatcaa
     5041 catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca catcttcctg
     5101 ggtgccaatc gggaaaccac caataccttc gtagcgacct ttaatcagca ggaggcgatc
     5161 gtcttccact tccagatgaa ccgttacatc cgggttagtc agcttcacgc gcgcggattc
     5221 aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc taaaatcatg
     5281 tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat cgcgatactg
     5341 aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt cgacttcgag
     5401 aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagtcagac gctggttttc
     5461 atctttcgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg tctcatcata
     5521 gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca agcgcacaga
     5581 ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg cggcaatggt
     5641 tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac acctgcggct
     5701 gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca ccttaccatt
     5761 gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa atattgagag
     5821 tcagacattc attatgccga agaaaaatga ggcgcccgcc agctttgaaa aggcgctgag
     5881 cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg aagaggcgct
     5941 gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat tacaacaagc
     6001 cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa ccccttttac
     6061 accggacaat gagtaatgga ctttccgcag caattcgaag cctgcgttaa gcaggccaac
     6121 caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt ggtcgaaacc
     6181 atgcagtatg gcgcattatt aggtggtaag cgcctgcgac ctttcctggt ttatgccacc
     6241 ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgctgc cgtagagtgt
     6301 atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga cgatctgcgt
     6361 cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct cgctggcgac
     6421 gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga agtgtcggat
     6481 cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc cggaatgtgc
     6541 ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga cgcgcttgag
     6601 cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct tggtgcatta
     6661 agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtacgc agagagcatc
     6721 ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac tgcaacgttg
     6781 ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc acttctgggt
     6841 cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca gtcgctgaaa
     6901 caactggctg aacagtcact cgatacctcg gcactggaag cgctagcgga ctacatcatc
     6961 cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga tattgccaaa
     7021 tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc gaaagagagt
     7081 ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag ccgttccagc
     7141 gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca ctatgtctac
     7201 aacaccccat ttgaccaatt gatttgggat gtgggacatc aggcttatcc gcataaaatt
     7261 ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggtct gcacccgttc
     7321 ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc aacctccatc
     7381 agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg ccgcaccgtc
     7441 tgtgtgattg gcgacggcgc gattaccgct ggcatggcgt ttgaagcgat gaatcacgcg
     7501 ggcgatattc gtcctgatat gctggtggtc ctcaacgaca atgaaatgtc gatttccgaa
     7561 aatgttggcg cgcttaacaa ccatctggct cagctgcttt ccggtaagct ttactcttca
     7621 ctgcgcgaag gcgggaaaaa agttttctct ggcgtgccgc ccattaaaga gctgctcaaa
     7681 cgtaccgaag aacatattaa aggcatggtg gtgcctggca cgttgtttga agagctggga
     7741 tttaactaca tcggcccggt ggacggtcac gatgtgctgg ggcttatcac cacgctaaag
     7801 aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa aggtcgtggt
     7861 tatgaaccgg cagaaaaaga cccgatcact ttccacgccg tgcctaaatt tgatccctcc
     7921 agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat ctttggcgac
     7981 tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc ggcgatgcgt
     8041 gaaggttccg gcatggtcga gttttcacgt aaattcccgg atcgttactt cgacgtggct
     8101 attgccgagc aacacgcagt gacctttgcc gccggtctgg cgattggtgg atacaaaccc
     8161 attgtcgcga tctactccac cttcctgcaa cgcgcctatg atcaggtgct gcatgacgtg
     8221 gcaattcaaa agctaccggt cctgttcgcc attgaccgcg cgggcattgt tggtgctgac
     8281 ggtcaaactc accagggcgc ttttgatctc tcttacctgc gctgcatacc agaaatggtc
     8341 attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg ctatcactat
     8401 aacgatggcc cgtccgcggt gcgctacccg cgcggcaacg cggttggcgt ggaactgacg
     8461 ccgctggaaa aactaccaat tggcaaaggc attgtgaagc gtcgtggcga gaaactggtg
     8521 atccttaact ttggtacgct gatgccagaa gcggccaaag tcgccgaatc gctgaacgct
     8581 acgctggtcg atatgcgttt tgtgaaacca cttgatgaag cgttaattct ggaaatggcc
     8641 gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg cgcaggtagc
     8701 ggagtgaacg aagtgctgat ggcccatcgt aaaccagtac ccgtgctgaa cattggcctg
     8761 ccggacttct ttattccgca aggaactcag gaagaaatgc gcgccgaact cggcctcgat
     8821 gccgccggta tggaagccaa aatcaaggcc tggctggcat aatccctact ccactcctgc
     8881 tatgcttaag aaattattca tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa
     8941 accgaccttc gcgtttcccg actttgcctc ggctgtatga cctttggcga gccagatcgc
     9001 ggtaatcacg catggacact gccggaagag aacagccgtc ccataattaa acgcgcgctg
     9061 gaaggtggca taaatttctt tgataccgcc aatagctatt ccgatggtag cagcgaagag
     9121 atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag acgtagtcgt tgccaccaaa
     9181 gtgttccatc gcgttggtga tttaccggaa ggattatccc gtgcacaaat tttgcgctct
     9241 atcaacgaca gcctgcgacg tctcggcatg gattatgtcg atatcctgca aattcatcgc
     9301 ggggattaca acacgccgat cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc
     9361 gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc tcaggcgctg
     9421 gaactacaaa aacagcactg ctgggcgcag tttgtcagta tgcaggatca ctataatctg
     9481 atttatcgcg aagaagagcg cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg
     9541 attccgtgga gcccgatggc gcgggggcga ctgacgcgtc catggggaga aactaccgca
     9601 cgactggtgt ctgatgaggt ggggaaaaat ctctataaag aaagcgatga aaatgacgcg
     9661 cagatcgcag agcggttaac gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt
     9721 gcgctggcct ggttgttaag taaaccgggc attgccgcac cgattatcgg aacttcgcgg
     9781 gaagaacagc ttgatgagct gctgaacgcg gtggatatca ctttgaagcc agaacagatt
     9841 gccgagctgg aaacgccgta taaaccgcat gcggtcgtag gatttaaata agagtccagg
     9901 cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc acaactacga cagaataccc
     9961 agcggccagt gatgaccgat aaagtacagg atgcctgcgg aaatcacccc ggcgacaata
    10021 tcgtcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg gatcggccac
    10081 ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcaaccca ctgccagtca
    10141 ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg
    10201 ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac aaagatagac gccgatacag
    10261 atccccaaca tcaccaccag cgagtagagc tgccagggta aaaaggtcat cagataccag
    10321 aaagggatcg ccgccagcga tcccatcgtg ccaggaacga ttgggcttaa tccacttccg
    10381 aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct
    10441 ttatggcgtg gcaaaatggt catatccttt ccagtcaaac gtgacaggtt cgccgtcacg
    10501 aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc
    10561 aaggtgtccg agagcaacat ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa
    10621 ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg
    10681 agaaagcaca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc
    10741 tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc
    10801 cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgat ggacggagat gacgtttgat
    10861 caagtagtca gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc
    10921 ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt tcgccccaga
    10981 gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca ttgataatgg
    11041 cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa
    11101 actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt
    11161 cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt
    11221 ataagccaga tcagcaggat cgatatcagg gaggaaatgg ttgcccgcca ccagcgtatc
    11281 agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat cgtcgccgat
    11341 gcccagttcg acatcaagac gagaacttct tacacggtca aaataacggg caatcaggga
    11401 gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg ccggcgatca
    11461 tggaacagtc ttccgtgaat ctaccggcct ggatattact ttttgttagg gcgaatcaca
    11521 ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc gccgaacgat
    11581 ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcact acgtttagac
    11641 agttcgtaca gcgcaatgcg cagtactgct ttttctacct ggcccagttc ttccagcagg
    11701 cgggacaggt agggcttcat cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc
    11761 agcagctcac ggaagtacag aacgtcaacg tcttttacat cctgttcagc caggaactgg
    11821 tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc ctggacggca
    11881 cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact aatttcaggc
    11941 cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt ctgcaccttt
    12001 gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag tggtcagaac
    12061 cccaaaagca accggaattt cgctgtcctg ggcaacatgc gccaggccgt tgcttgcacc
    12121 accagcgaca tattcaaagt gggcagagcc accacgaata accgtaccca gcgcaatcac
    12181 cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct cataggcacc
    12241 aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc
    12301 aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga tggtgatggc
    12361 gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct tccttcgggt
    12421 tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc tgcttccctt
    12481 tcgagccggg agatcatgcc cccactaaat gcaggcaaac atccgggcct acatgacgta
    12541 tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc aagccctggc agcgagcata
    12601 atccacgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagt tcatcgacta
    12661 aacccgcctg tagcaatacg ccagcgagcg ttggccccgc ttccacccag atgctgttaa
    12721 tctgctgttt acccagttgc atcatcagta caaccagatc cagatgacct ttatgctctg
    12781 gaatcagcaa ggtacgcacc gtttccggcc actcacgaga atcttcctgc gtacgcgcga
    12841 accaggtttc gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga ttttggctat
    12901 caatcacaat acgtactggc tggcggagat tttgttgcgg gtagagcgcc tgagtttgtt
    12961 catccagttc agaccaacgc actgttaagg ctggatcatc cgccagcacc gtggcgctgc
    13021 tggttaaaat ggcatgactt tgtgcgcgct gtcgttgtac gtcgcgccgc gcctgaggcg
    13081 aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg gccatcaagc gatgcgccaa
    13141 gttttaactg aatataagga aagccggtgc gcatccgctt gagaaagcct ttattcaatt
    13201 gctcggcttc actcatcatc agcccgtggc tgacgtcaat gccagcctgt tgcagacggt
    13261 aaagtccacg cccagcgacc tgcgggttag gatcttgcat cgctgcgacc acgcgcgcta
    13321 cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg gccatgatgg ctacagggtt
    13381 cgagtgtcac atacgccgtt gcgcctttgg ctttttcacc cgccatacgc aacgcgtgta
    13441 cttcggcatg tggttcaccc gctcgttggt ggtaaccttc accgacaatt tcgccatctt
    13501 tgacaatgac gcacccgaca ttcgggttgg ggtgggtggt aaaacgtcct cgttgcgcca
    13561 gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc aggcgcgcga
    13621 tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca aaacggatat
    13681 aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc atcttgctcg
    13741 gtacttcgcg ctcaccggtg gcgcgcagct gcgatttaat atgattgatt gccatttcaa
    13801 cgtaatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta cgcaatttct
    13861 cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata accagctccg
    13921 ccacttcaaa ggtggtgaaa cgttcattac acaccagaca ctgccgacgg cggcgtacgg
    13981 atgaaccctc gcccacgaga cgagagtcaa ttaccttagt gtccacggcg aaacagaatg
    14041 ggcaatgcat acggcgtcct gaccaggtgg ttaacggaaa tctattttac cctgaactga
    14101 cgatacaaca aaggcgcaac gcttttgaga taaaggatcg atgctttcgc tgcttttacc
    14161 ctctaccatt aggctaattg cgacgagaaa gggacgacct atgaatacta acgtttttcg
    14221 actgctcctg ctgggaagcc tgttcagcct tagcgcctgt gtgcagcaaa gtgaagtgcg
    14281 acagatgaaa cacagcgtca gcacgctgaa ccaggagatg acgcaactca atcaagaaac
    14341 agtcaaaatc actcagcaaa acaggctgaa tgcaaaatcc agcagtgggg tttaccttct
    14401 gcctggagcg aaaacaccgg caagactgga aagccagatc ggtactttac gtatgtcgct
    14461 ggtgaatatt acgcctgata cagatggcac cacccttaca ctacgtattc agggtgagtc
    14521 gaatgatccc ttgcccgcat tcagcggaac tgttgaatat gggcaaattc agggaacaat
    14581 agacaacttt caggaaatca atgtgcagaa tcaattgatt aatgcccctg ccagcgtcct
    14641 cgcccccagc gatgttgata ttccgttaca attaaagggg atttctgtgg atcagttagg
    14701 ctttgtacgt atccacgaca ttcaacctgt tatgcagtaa acgtattttg ggacgatttt
    14761 gtgcgtcccg caacatcttt ccccgtcatt ttgttactct gcttacatca cttggattga
    14821 tagtaaaagt ttgcaacaag ggcgaaagtc agtacaatcc ccgcccgaat gtgtgtaaac
    14881 gtgaacgcaa tcgattacgt aaatgataga actgtgaaac gaaacatatt tttgtgagca
    14941 atgattttta taataggctc ctctgtatac gaaatattta gaaacgcaat ttgcgccttt
    15001 ttcactcccg caagggattt tcaaacagtg gcatacatat gaaaaaaaca ttactggcag
    15061 ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa cgcagctgaa aacgacaaac
    15121 cgcagtatct ttccgactgg tggcaccaga gcgttaacgt tgtcggaagc tatcacaccc
    15181 gtttcggacc gcagatccgc aacgatacct accttgagta cgaagcattc gctaaaaaag
    15241 actggttcga cttctatggt tatgcggatg cgccggtatt cttcggcggt aactccgatg
    15301 ctaaaggtat ctggaaccac ggttctccgc tgtttatgga aatcgaacca cgtttctcca
    15361 tcgacaagct gaccaatact gaccttagct tcggtccgtt caaagagtgg tacttcgcga
    15421 acaactacat ttacgacatg ggtcgtaata aagatggtcg ccagagcacc tggtacatgg
    15481 gtctgggtac cgatatcgac actggcctgc cgatgagcct gtccatgaac gtctatgcga
    15541 aataccagtg gcagaactat ggcgcagcga acgaaaacga gtgggacggt taccgtttca
    15601 aaattaaata ctttgtgccg attaccgatc tgtggggcgg tcagctgagc tacatcggct
    15661 tcaccaactt cgactggggt tccgatttag gggatgacag cggtaacgca atcaacggta
    15721 ttaagacccg tactaataac tctatcgctt ccagccatat tctggctctg aactacgatc
    15781 actggcacta ctctgtcgta gctcgttact ggcacgacgg tggtcagtgg aacgacgatg
    15841 cagaactgaa cttcggcaac ggcaacttca acgttcgctc taccggctgg ggtggttacc
    15901 tggtagtagg ttacaacttc tgattatgaa aatgccggga tttattcccg gcatttctga
    15961 ttgttaacaa catgtgcttt gttcatgccg gatgcggcgt aaacgctttg tccggcccac
    16021 aaaagcacgt aaattcaata tattgctgaa ataacgtagg cctgataagc gtagcgcatc
    16081 aggcaatttt acgttttaat cacttcttct tgttcatcat cgctttcaga tcagcgaatg
    16141 ggttgtactt cgcttcaccg acatctttct gcgcatcttc ccctgcgata acggtcgtac
    16201 catactgatc cgcttcggta tatttcgaat gctcatgatc gtggcaatag agacacaaca
    16261 attcccagtt actgccatct tccgggttat tggtatggtc gtgatcaatg tggtgaacgg
    16321 taagttcacg caggttagaa taaacaaact cgcgggaaca gcgaccgcag acccacggat
    16381 agatttttaa tgctttttcg cgatagccgc tttctaaccg tgcatagttt tttgggatga
    16441 tagccatgaa aagttacctg gatgaaatgt agggttacgc gcagtttccc aagatgcggg
    16501 gattttcgca atctccatac atcaaaaaaa atcccgatct tctgaccggg attttcaaca
    16561 tcaacgggaa cttgattaag gcagaattga cggctgatcc gccccttctt tttccacttt
    16621 ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccaacgcag atgctacgta
    16681 gatggaagat gcagtgccga tggaaacacc gataagcatg gtcagcgaga agccttccag
    16741 taccggacca ccgaagagat acagcatcag gataaccatc aaggtagtac cggatgtgat
    16801 caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg
    16861 acggatcttg cggaagtttt cacgaattcg gtccgatacc acgatactgt cgttaagcga
    16921 gtaaccgata accgacatca acgatgccac aatggtcagg tcaatctcga tatggaataa
    16981 cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc gcaataacca cccctgccgc
    17041 cagtcgccac tcaaagcgga aacctacgta cacgaggata gacagcagcg ctgccatcaa
    17101 cgccatcgca ccggtttgcg caaggtctgc ccccacgctc ggaccgacga actcaatacg
    17161 cttcactgct gcattctgat tggtggattc gttaatcacc ttcagaactt ggctgcccag
    17221 cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt cgtggctgct
    17281 accaaagttt tgcagcattg gctcttcaaa accggctttt tgcaatgcat cacgcattac
    17341 gtcaatttca gccggttttt cgagcgtaat ttcgataacc gtaccaccgg tgaaatccag
    17401 cccccagtta aagccgcgca cgcccataat aacgatagca gcgattaaca gcagaccaga
    17461 gatgccgaaa gcccagtagt cccagcgcat aaagtcatag actttacggc cgtggtttag
    17521 ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc ttcttgacgc
    17581 gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg
    17641 acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg gtacccactg
    17701 cgtacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagata gaactgaatg
    17761 cgccacgata accttcatca attgcctgtt gaacagtacg tccgttgctc aactcttctt
    17821 taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt aagacgatac
    17881 ccgcgatacc tggcatactc agtgttgcgc ctggcaacag cgacataata ccgacgatca
    17941 agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta tagaagatga
    18001 tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc tgctcaatgt
    18061 tctgcatacc cagggtcggg ccaattgtac gttcttcaac aatctgaatc ggcgcgatca
    18121 acgcaccggc acgcagcagc agtgacagct gacgggcttc gttcgggttg ttgatgccgg
    18181 tgatacggaa gctgttaccc agacgagact ggatgttggc gatgttaatc acctcttcct
    18241 gtttcaccag aaccgcacga ccatttgcat ctttcttacc gctgtctttg tactccacaa
    18301 acagggttgc catcggtttg ccgatgttgt ccttggtgaa gttagacata atgttgccac
    18361 cagcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg
    18421 agtcggtgat atggtcacca gtcagaatta cgcgtttgta cagcacaacc ggctgacctt
    18481 cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat
    18541 caacattggt gtttaccaga cggaattcca gcgttgccgt cgcgcccaga atctctttcg
    18601 cacgcgcagt gtcctgaata ccaggcagtt caacaacgat acggtcagca ccctgacgct
    18661 gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata ttaatgttct
    18721 gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc gcacgcagct
    18781 ggttgctgcc ctggctgcta atcaccaggt ccggatggcg cttgctcaga tacgcaatgg
    18841 cttcatcacg agctttagca tcgcggaaag tgatgctcag gccgtagttg ttttctttac
    18901 gaacagtggt atacgggatg cccttttcgc gcaggtcact gcgtaggcta tcgatatttt
    18961 gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag tgaacgccgc
    19021 cacgcaggtc aaggccgagc ttcatcggct cagcatgaat agctgccagc cagcgcggcg
    19081 ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc attaatgctt
    19141 cacgagcgcg caactgggtg tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca
    19201 gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc tggatcagcg
    19261 tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa
    19321 acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc atgacgtact
    19381 tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt aattacagcg
    19441 ccttcatggt gcctttcggc aggacggcag ctacgaagtc acgtttaata actacttcag
    19501 tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc tactttggtt acgcgaccaa
    19561 ccaggccacc gttcgtcaga acttcatcac ctttggcaat ggagtccatc agctttttgt
    19621 gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc agaccgaata
    19681 ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc gttgccgcta
    19741 ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta atcaacgttc
    19801 aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct ctctaattta
    19861 ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg aaggttatga
    19921 atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt cgagatgatg caagtaagcg
    19981 cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc agtatcgctc
    20041 ttatatttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg accatttcgg
    20101 gcgttgcggg ttggcattac gcagtcaaac atatcgatac cgcgacgtac gccttcaacc
    20161 aggtcttccg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg aatttgcgga
    20221 cacacatgct ccagaatacg gtgcatatct gctttcggct cacccacagc cagaccgccg
    20281 acagcgtagc catcaaagcc gatatctacc agacctttaa cagatatatc acgtaaatct
    20341 tcgtaaatac tgccctgaat tataccaaat aacgcatttt tgttaccaag actgtcaaaa
    20401 cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tagagcgttt tgcgtaatcc
    20461 cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga accaagatcg
    20521 tactgaatct ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggtta
    20581 cggaagtgaa cgccctgttc ggtgatttta cgaatatcac caaggctgaa gacctggaag
    20641 ccgccggagt cggtaagaat cggtcctttc cactgcataa aatcgtgcat atcgccgtgc
    20701 agtttcatga tttcctggcc cgggcgcagc cacaggtgga aagtgttgcc gaggataatt
    20761 tgcgcgccag tggcttcaac ttcttccggc gtcatccctt ttacggtgcc gtaggtgcca
    20821 acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg gccacggcgt
    20881 gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg tcagaaaaac
    20941 agtccaacgt ttaaaccagc gccgcggaat tactccccga cgcgctcatt aattgcctgc
    21001 ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt ctcttctacc
    21061 gccgctttat aggcattcat ggtgtgttga taaccggcaa aggccgaaac cagcataatc
    21121 agcgtcgact ctggcaagtg gaagttcgtc accagcgcat cgaccacttt gtactggaag
    21181 cccggataga taaagatttg ggtatcgtca aagaacggtt cgataagatc gttttttgcc
    21241 gcctgtgccg cgctttccag cgaacgtact gaagtggtgc caaccgcaat cacccggtta
    21301 ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggtacttc agcgtattcc
    21361 gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca
    21421 acgtgcaacg tcacaaacgc catctccacg cctttggcgc gtaatttttc cagcaaaggc
    21481 tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc gctataaacg
    21541 gtttgataaa gttcgcggtc agcgtcttcg tccggacgat cgatatacgg cggcagcggc
    21601 atatggccga tgctgttgag gatatccagt accgagcgtt catcattaaa ttcgacttca
    21661 aacagtgcgc cgtggcgcgc ggtcattgtt gcgttgatac tttcgtcatc gcccagcaac
    21721 agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg
    21781 tcgagcatcc gctcaaccag cacttcaatc ttaccgccgc tggctttacg cccaaacaga
    21841 cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt gagcttatcg
    21901 agtaaatcgg tgaaagtacc gtgcgtcagc gcacccgtcg gcccgtccag cgacagtaaa
    21961 cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg caattcaaag
    22021 gagaaatcgg taacgcgcat gacactgact cagactaaaa taagagtcgg gtagtctagt
    22081 gccggggcgc tctccctgca acctttaccc ccctttggat agaatataat cgatgaattt
    22141 tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttctggca atttactggc
    22201 tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca
    22261 tatgcatcga cgtattgact gacaatctgc cggaagtccg cgaagcacgg gagtggtttc
    22321 gtagtgaaac gcgccgcgtt gcgcctatta cgctggatgt catgtgggat cactttcttt
    22381 cccgccactg gtcgcagctg tcgccggact ttccactaca ggaatttgtc tgttatgccc
    22441 gcgagcaagt gatgacgatt ttgccggact caccgccacg ttttatcaat ctgaacaatt
    22501 acttgtggtc agagcagtgg ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt
    22561 taaacggcat ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc tggtacgatt
    22621 tagacgctca ttatgacgcc ctcgaaaccc gcttctggca gttttatccg cggatgatgg
    22681 cgcaggcgtc acacaaggcg ttataaaacg ttagttcatc cataccgtag ccgaaatggc
    22741 aggcaaagcc agaatcccgt cagtcagttg cccatgccct tctttgcatt gccattgcac
    22801 ggcattgaga aacggtgacg cgggtagcac cacttcactg gcctcgccac ggttgattgc
    22861 caccagtaca cgttgctgat tcagcacgcg gacaaatacc actacgttat cttccgcata
    22921 cagcacctga cagccgccac gacgcagcgc ctgacttttc ttacgcagca caatcattcg
    22981 ccggtacagc gcgaataacg ccgtatcctg cttttccacc tgccagggga acggtttacg
    23041 gcaagacgga tcgtttttac catccagtcc cacttcatca ccgtaataaa tgcacggtac
    23101 accaggccag gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat cccgaccgag
    23161 cagcgtttta aatcgcgcag tatcgtggct atcgagctga ttaaacatac gtaattgttg
    23221 ttgatgagaa agccctgcgc ggtaattatc catccaggcc atacaggttt gggcgtcaat
    23281 ttgctgcgga tcataagaga tgtcggtatt ggcaagaaat ccccacaacg ggaatgtgaa
    23341 gccacgatag ttcatggcag catcttccac atcggcctgt aaccattgcc gtgcatcgcc
    23401 aaaatgttcg ccgacaatat acgcttccga ctgggtttct ttcgccgctt cggtgatccc
    23461 ggcaacgtgc tgcatattat tgcgcgcccc acccgcctcc cccagcatat gcaccacatc
    23521 cagccgccag ccgtccatat tccacggcgc tttcagccag tggcggacaa tactgtcttc
    23581 cccgcgataa atttcattca ccagactttc cgactgataa tccagcttcg gcaagctggc
    23641 atagccaagc cagtcgagcg ccgtgccatc atcactaaac aagtaccagt cgcgccaggg
    23701 cgattcagag ttgtgacaag ctccccccgt gccaagattg tgcctgtca
//
LOCUS       DAJTRQ010000053        23583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000053 DAJTRQ010000000
VERSION     DAJTRQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="OO332_002171"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="OO332_002171"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5942645.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="OO332_002172"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="OO332_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5942646.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="OO332_002173"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="OO332_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5942647.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="OO332_002174"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="OO332_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5942648.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="OO332_002175"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="OO332_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5942649.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="OO332_002176"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="OO332_002176"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5942650.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="OO332_002177"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="OO332_002177"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5942651.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="OO332_002178"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="OO332_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5942652.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="OO332_002179"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="OO332_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5942653.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="OO332_002180"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="OO332_002180"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5942654.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="OO332_002181"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="OO332_002181"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5942655.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="OO332_002182"
     CDS             10418..10882
                     /locus_tag="OO332_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5942656.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="OO332_002183"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="OO332_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="OO332_002184"
     CDS             11895..13298
                     /locus_tag="OO332_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5942657.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO332_002185"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO332_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5942658.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO332_002186"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO332_002186"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5942659.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO332_002187"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO332_002187"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5942660.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="OO332_002188"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="OO332_002188"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5942661.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO332_002189"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO332_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="OO332_002190"
     CDS             complement(20164..20457)
                     /locus_tag="OO332_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5942662.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="OO332_002191"
     CDS             complement(20454..21743)
                     /locus_tag="OO332_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5942663.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="OO332_002192"
     CDS             complement(21799..22737)
                     /locus_tag="OO332_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5942664.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="OO332_002193"
     CDS             complement(22757..23521)
                     /locus_tag="OO332_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5942665.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5812 a         5761 c         6213 g         5797 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttctcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aat
//
LOCUS       DAJTRQ010000054        23326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000054 DAJTRQ010000000
VERSION     DAJTRQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="OO332_002194"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="OO332_002194"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5942666.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1493..1906
                     /gene="hns"
                     /locus_tag="OO332_002195"
     CDS             1493..1906
                     /gene="hns"
                     /locus_tag="OO332_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5942667.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OO332_002196"
     CDS             complement(2050..2958)
                     /gene="galU"
                     /locus_tag="OO332_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5942668.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OO332_002197"
     CDS             complement(3160..4173)
                     /gene="rssB"
                     /locus_tag="OO332_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5942669.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OO332_002198"
     CDS             complement(4265..5170)
                     /gene="rssA"
                     /locus_tag="OO332_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5942670.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5283..5741
                     /locus_tag="OO332_002199"
     CDS             5283..5741
                     /locus_tag="OO332_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5942671.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5791..6633
                     /gene="purU"
                     /locus_tag="OO332_002200"
     CDS             5791..6633
                     /gene="purU"
                     /locus_tag="OO332_002200"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5942672.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6793..6877
                     /locus_tag="OO332_002201"
     tRNA            6793..6877
                     /locus_tag="OO332_002201"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6827..6829,aa:Tyr,seq:gta)
     gene            6924..7055
                     /locus_tag="OO332_002202"
     ncRNA           6924..7055
                     /ncRNA_class="other"
                     /locus_tag="OO332_002202"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7239..7916)
                     /gene="narI"
                     /locus_tag="OO332_002203"
     CDS             complement(7239..7916)
                     /gene="narI"
                     /locus_tag="OO332_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5942673.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="OO332_002204"
     CDS             complement(7916..8626)
                     /gene="narJ"
                     /locus_tag="OO332_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5942674.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8623..10161)
                     /gene="narH"
                     /locus_tag="OO332_002205"
     CDS             complement(8623..10161)
                     /gene="narH"
                     /locus_tag="OO332_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5942675.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10158..13901)
                     /locus_tag="OO332_002206"
     CDS             complement(10158..13901)
                     /locus_tag="OO332_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5942676.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14294..15685)
                     /gene="narK"
                     /locus_tag="OO332_002207"
     CDS             complement(14294..15685)
                     /gene="narK"
                     /locus_tag="OO332_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5942677.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16024..17820
                     /gene="narX"
                     /locus_tag="OO332_002208"
     CDS             16024..17820
                     /gene="narX"
                     /locus_tag="OO332_002208"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5942678.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17813..18463
                     /gene="narL"
                     /locus_tag="OO332_002209"
     CDS             17813..18463
                     /gene="narL"
                     /locus_tag="OO332_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5942679.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="OO332_002210"
     CDS             complement(18464..19858)
                     /gene="ychO"
                     /locus_tag="OO332_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5942680.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20043..20396
                     /gene="ychN"
                     /locus_tag="OO332_002211"
     CDS             20043..20396
                     /gene="ychN"
                     /locus_tag="OO332_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5942681.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="OO332_002212"
     CDS             complement(20440..21135)
                     /gene="chaC"
                     /locus_tag="OO332_002212"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5942682.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="OO332_002213"
     CDS             complement(21293..21523)
                     /gene="chaB"
                     /locus_tag="OO332_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5942683.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21793..22893
                     /gene="chaA"
                     /locus_tag="OO332_002214"
     CDS             21793..22893
                     /gene="chaA"
                     /locus_tag="OO332_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5942684.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5853 a         6084 c         5611 g         5778 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tatatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
      361 ttcattacca ccaattacca cctgctcacc ttcgttataa ggtctgcctg tttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca taatccttct
     1021 gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactgct ggtaactatt
     1081 cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg agatagtgca
     1141 atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa
     1201 ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga taatctcccc
     1261 ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa
     1321 atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca
     1381 caaaataaag aacaattttg aattccttac attcctggct attgcacaac tgaatttaag
     1441 gctctattat tacctcaaca aaccacccca atataagttt gagattacta caatgagcga
     1501 agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact
     1561 tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaatgaac gtcgcgaaga
     1621 agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc aatatcgcga
     1681 aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa
     1741 atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa
     1801 cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca aaaaagcaat
     1861 ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg
     1921 cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa gacaatttaa
     1981 taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg gataacaccg
     2041 atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa attccgtgcc
     2101 aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta atttattacc
     2161 gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt cgatcagcat
     2221 atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt ttgccagcaa
     2281 cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag acggcgcaac
     2341 atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg gcgctaattc
     2401 aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt caaccatgat
     2461 ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat cctgtgacaa
     2521 atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg gttcatcacc
     2581 cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac cctgacgaac
     2641 ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca gttgacgttt
     2701 tacacgtttt tccagcattg cctccagttc aaaactggta tcaaagtggt tttcaataga
     2761 gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa tacattcatt
     2821 cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg ggatggcttt
     2881 cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt ttttgacttt
     2941 cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac gtgttcatat
     3001 ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga aaaggaggcg
     3061 agatagcata attaagacaa ttacttatac gaatggcgac gatagtacca ccagccgaca
     3121 ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag acaacatcaa
     3181 gcgcagtcga ccaccggttc cccatatttg gcattgccaa gcatcgcatc gctggctcaa
     3241 ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga tttgcacctg
     3301 gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga gatttttcag
     3361 ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg cctgacgaag
     3421 taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat tttggtgtgc
     3481 aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag caagtacgcc
     3541 attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt ccgaaagtgc
     3601 ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacggt aattaacccg
     3661 gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt tcgccgctgc
     3721 ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt cctcaacgcg
     3781 cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc gattcagatc
     3841 tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg caatatctgc
     3901 catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt tacgtatatg
     3961 ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca tcaggtctgg
     4021 agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta ccgttgtcgc
     4081 tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat cttcaacaat
     4141 gagaatctgt tttccgacca atggctgcgt catgttctct cccctgaccg gctttactca
     4201 atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta gataaaaatt
     4261 ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt tccactgcca
     4321 gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat atttgcgggc
     4381 aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt ttaaggcggt
     4441 tctccaacac ctggatagac gtggtcataa tctctgttgc cgttggcgct gtcacagccc
     4501 gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggt agagaatcac
     4561 cattctcgct attttcttca ctgacattaa aggagagcag atcttgttgc atcaaatgag
     4621 catcgtgctg caggtcgacc gctatcacaa tatcagcccc caatgcacgc gtaagagaaa
     4681 taggaattgg gttaactact gccccatcaa ccagccagta gccgttatgt gcaacgggtg
     4741 ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg ccttcagtaa
     4801 accataattc acgccccgta cttaaattgg tggcaacagc cgcaaagcga cgggaacaat
     4861 tttcgatctc tgtttccggc attatttcgc gatattgatt gaagacacgc tcgccgcgca
     4921 gtaacccacc gcgctgccag gagaggtcca tcaggcgtaa aacgtcccaa taactgaaag
     4981 aggttaccca atcttccagc gcagataatc gatcgcatgc ataggccgcg cccaccagcg
     5041 aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt agcgcattaa
     5101 taacgccaat atgcgaccaa cctctcgccg cgccagatcc cagcgccagc cctattttta
     5161 tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca tcgctactgc
     5221 tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca gggagattat
     5281 tggtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt tgccaccctt
     5341 atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg cgttactgcg
     5401 cttttgtgat gcaagacgct gattatttaa taaagacctg gcatccctct tgtggagcag
     5461 cggcgttacg tgccgaattg atgaccggat ttgcgcatac tgaatggttg ggattgaccg
     5521 tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt gttgctcgat
     5581 ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc gcgatttttg aaagaaaacg
     5641 gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat ccctgccctt
     5701 gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg gtacggttta
     5761 gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa agttctgcgt
     5821 actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg ctacaagcac
     5881 gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg cttttttatg
     5941 cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct cgatagcgca
     6001 ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat agtgattctg
     6061 gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta cggcggtctg
     6121 gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct ggttgagcgt
     6181 tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga gcacgatcaa
     6241 aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc gaagtatatg
     6301 cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa tattcaccat
     6361 tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga acgtggtgtg
     6421 aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg cccaatcatc
     6481 atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat gcgcgcaggt
     6541 cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc gcaacgcgtc
     6601 tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac tggttacctt
     6661 taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta acactttaca
     6721 gcggcgcgtc atttgatatg atgcgccccg ctccccgata agggagcagg ctagtaaaaa
     6781 gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa atctgccgtc
     6841 atcgacttcg aaggttcgaa tccttccccc accaccatca ctttcaaaag ccccggaatt
     6901 ctcaaacgaa tctgcaatca aatattctgc ccaagcgggg aaggatgaga agcttcgacc
     6961 aaggttcgac ttgagcgcca gcgagagagc gttgccgcag gcaacgaccc gaagggcgaa
     7021 gcgcgcagcg ctgagtaatc cttcccccac caccatcgct tccttgaata atcaaaacct
     7081 gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca atatcctccc
     7141 ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc ccataaaaaa
     7201 accccgccga agcagggtct atgtgaacta aaattcgttt agtgacgagc gcgcaccagc
     7261 tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag acgcgagaac
     7321 gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat aaacgccaca
     7381 ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc ccagccaacc
     7441 agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt cagcaggccc
     7501 agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt agtggttgca
     7561 cgcacgcgtg ggctgaacat acgacgtttc agcaaaagca cgccgccaat cagacacagc
     7621 acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat cggcagccag
     7681 gcttcataca tccagtgcgg cgtcaacata ccgaagaagt gaccgacaaa aatccccaga
     7741 ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag catctggctg
     7801 gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc aatcaggaag
     7861 accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa ttgcattagt
     7921 gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca aagcgacgct
     7981 gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta acctgctctt
     8041 cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg gcaatttttt
     8101 ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga tcaaacatca
     8161 cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga atcggcgcga
     8221 tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac tccagataca
     8281 gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa gccgtgctgc tcgtactgcg
     8341 ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca tgttcaaata
     8401 gcaacagtga cgtggcgcgg ccacggtcga acagttcgct gtactggacc tgggcatcga
     8461 gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca tcttctttcg
     8521 acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc cataaggcag
     8581 catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat gcggctccgt
     8641 tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga atttggtatc
     8701 tgagccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg ggaaggcttc
     8761 ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt tagcaatcgc
     8821 cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt ccagcgcacg
     8881 ggtatcaact ttaccgtcaa cagtttcagc acgtttgtaa tgacgcatcg ccagcatacg
     8941 tttcagcgca cgcagtaccg gtttggtatc accggcggtc agcagattcg ccagatactg
     9001 taccggaata cgcaagcttt cgacgtctgg cagaattccg ttgctgccca gctcgcccgc
     9061 gtctgctgca gactgaatcg gagacagagg cggcacgtac cagaccatcg gcagtgtgcg
     9121 atattctgga tgcagcggca gcgccagttt ccattccatc gccattttat aaaccggcga
     9181 ctgctgtgca gcttcaataa cgctcagcgg aataccgtct ttaatcgcct gctcgatgac
     9241 tttcggatcg ttcggatcga ggaacacgtc cagctgacgc tggtagagat ctttctcgtt
     9301 ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc caagataacg
     9361 gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa tacgcggata
     9421 gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt ttttgtacgg
     9481 acatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca ggacgatgcc
     9541 atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg ccgggttcag
     9601 gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact ggctatacat
     9661 cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttatcaaact caccgcccag
     9721 gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg taatcagcga
     9781 acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca ggttctgata
     9841 atcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt tagcaaagat
     9901 tttacccagc agcatggctc ggttacccat gcgcggttgc agtttgccat tgattttacg
     9961 gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc cctggcccgg
    10021 cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg tccagacgtt
    10081 tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca gcaccatgcc
    10141 gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc ttcgccatct
    10201 aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt agaacctacg
    10261 gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat atgcgtcggt
    10321 ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt aatttccgaa
    10381 ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc tgccggaaca
    10441 cgctggctga caaccgcacg ggcagtcagt gcaccgttgc tgttgaagac ttcaatccag
    10501 tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac caccggacca
    10561 ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat accccacttc
    10621 tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt ctggcctatc
    10681 acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct ttcaccgaaa
    10741 tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt acgccatggg
    10801 atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc ttccagacca
    10861 gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg gaagcggatc
    10921 ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa ttcgctcagg
    10981 gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagcgt caggatcatc
    11041 tctgccgcat caattgcggt gttcagcatc ggctggcctt ttgccggacc ttccgctttg
    11101 gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc aatcccttta
    11161 ccgccgttac cgattttctc catcagcggg ccgatagagg taaagcgttc gtaagttgcc
    11221 ggataatcgc gctctacgac cataatgtgc ggtgcagttt tgcctgggat caggtcgcat
    11281 tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc agagtcgtgc
    11341 tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca cacttcggag
    11401 aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tagcttccca ggccggatcg
    11461 accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat gtcgtctttt
    11521 tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct cgacagacgg
    11581 aagtccagcg taaccaccag atccagcttg ccttccagac cattgtcctg ccagtccact
    11641 tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc gtgctccgtc
    11701 cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag caggttagaa
    11761 cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc tgccgcaaaa
    11821 cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat cccggctttt
    11881 tccgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggtgcaga cggcagccag
    11941 cccatgcgtt ccgcacggac gttaaagtcg atcaagtgtc cggtatagcg ggatttgtcc
    12001 gccatcggtg acagcaactc ttccgccgtg acggtttcat aacgccactg gctggagtgg
    12061 ttatagaaat aagaagtgct gttcatgtga cgcgccggac gctgccagtc aagggcaaac
    12121 gccagcggct gccagccggt ttgcggacgc agtttttcct gacctacata gtgcgcccag
    12181 ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag accacgatag
    12241 ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat cgaacgaccg
    12301 tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg gctgcgagaa
    12361 acgccggtaa tctgctcggc ccaggccggg gtataagctt tcacatcgtc atagctggtt
    12421 gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt cagatcataa
    12481 acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac cggcagtttg
    12541 tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc gccaccaaag
    12601 tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag ttgcagctcg
    12661 gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt ctcgccccag
    12721 cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa ggcgacagtt
    12781 ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc gcgcagcata
    12841 cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg catgtcggtg
    12901 tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa ttcacgcagc
    12961 attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg tgccagccac
    13021 agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt tttggtgcct
    13081 ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg cacgtttgaa
    13141 ccccaggcga tgatgtagct ggagttatac cagtcagcag attccggtac gtcagtttgc
    13201 tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt accagtcgta gaagcttaag
    13261 caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac catcgacatt
    13321 gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat ggtgtaaacg
    13381 ttagatgcgg cgatcagttc gttcacctcc tgccaagaag aacgaacaaa tccaccgcgt
    13441 ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga agcccatgcc
    13501 tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag gcgtttgcgc
    13561 atcatcgggt atttcaggcg gttggcactg taaagatacc aggagtagct ggcaccgcgc
    13621 gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata gtcagtctgc
    13681 tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca ggagccggtg
    13741 cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg gcgatatcca
    13801 tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa ggtttcaccc
    13861 ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct cctgtgggag
    13921 cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta acgctctgaa
    13981 acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag taaattccta
    14041 agatacccct ttaggataat taacagttac agcataacat gctgtaaatt aagacgtttt
    14101 atacctgtag ttatttaaac tattaagatt tttatgcaaa tgggtattaa ggagtattcc
    14161 ccatgggtaa catattaatg gctcttaatg aaatccggcg aaataagcca tataacacta
    14221 atatgttgta actaaatgac gcataaaaaa agcgcggcat catgccgcgc aaaggataat
    14281 caaatattaa gatttatttt ttagaatgcc gaccatatac cgcccaagta atcactacgc
    14341 aggcgatata gaaaatcaaa aatactttca ttgcacctac tggcgaaccc gttaatgcca
    14401 gcgagctacc aaacgctttc gggataaaga agccaccaat cgcgccaatc gcagagatga
    14461 aacccagcgc cgctgccgtg ccggttgccg cttcacgcat cgcacgttcg tcagaacccc
    14521 cttctgcttt cacgcgatcc attgtcagtt tacggaagat cactgaaatc atctggaaag
    14581 tggaaccact acccagccca gctgtcagga acagcgccag gaagaccgcg aagaacgcca
    14641 tgaagcttcc gccctgcccg tcagtcggta aggtcaggaa cagcaggccg ctgaaaatcg
    14701 ccatcagaat aaagttcacc agcgtgacac gagttccgcc cagacggtca gataatgcac
    14761 cacctgcaga acgcgccagc gcaccaataa acggcccgaa gaaagcgtat tgcagaatct
    14821 gaacatccgg gaactgcgtt tttgacagca tcgcaaagcc cgcggagaag ccgatgaagg
    14881 agccgaaggt tgccagatac agcaggctca taatccacag atgaccccgt ttgagtaccg
    14941 gcaactgctc cttgatggag gctttcgagg tagcaagatc gttcatgcca aaccacgccg
    15001 caatggtgaa gatggcaagg aacggcaccc atatccagga cgcattcgcc agatacagct
    15061 cagtcccatc cggctgtttg acgccctggc taccaaatac tgcgaaaatc gacagtgata
    15121 ccaccagcgg agcaaccaac tgcatgacgc tgacgcccat gtttcccaga ccaccattca
    15181 gacccagcgc gccaccctgc ttctgtttcg gaaagaagaa gctgatgttt gccatactgg
    15241 atgcgaagtt cgcgccagca aaaccgcata gcagagagat gatgatgaag acgctataag
    15301 gcgtggaggt atcctgcacg gcaaaaccca gccagacgca aggaataatc agaataccgg
    15361 tgctgaacgc cgtccagcga cgaccaccga agataggaac cataaaggag tatggaacac
    15421 gtaataacgc gccagaaacc gaaggcagcg cagtcaacat aaatagctga tcggtcgtaa
    15481 aattaaaacc gactttcggt aagttcaccg caacagcgct gaacaacatc catacgcaaa
    15541 acgccagcag cagacaggga acggaaatcc acaggttgcg gctggcaata cgttgaccgc
    15601 gttgttgcca gaacgcagga tcttccggtc gccaatctgt tatgacagct ccagtagccc
    15661 tttcggaggc agatgagtga ctcatagaca cctctgatac tcgtttcgat gtctgccacc
    15721 ttagtgtctg gagctaaagg caatttgatg taaatcaaac gataagataa ctttatctat
    15781 cattgatatt tatcattacc catagtgagt acagtgattt cataaaaatt atgagatttt
    15841 tcacggtgct gtagaatccc tacccttacc gatgtaaagc gactaaccac acggcaaata
    15901 aggagtaact ctttccggct atgggtatac ttcagccaat agccgagaat actgccattc
    15961 cagaatatat cgtcacattc attaaggtta ttgctcattt aaagcctgaa ggaaggggtt
    16021 tacatgctta aacgttgtct ctctccgctc accctggtta atcaggttgc gcttattgtg
    16081 ttgctttcta ctgctattgg actggcaggg atggcggttt ctggctggct ggtgcaaggc
    16141 gttcagggca gcgcccatgc gatcaacaaa gcgggatcgc tgcgcatgca aagttaccgt
    16201 ctgttggcgg cagtgccatt aagcgagaaa gacaagccct taattaaaga gatggaacaa
    16261 acggcattta gcgccgagtt gactcgagca gcagaacgag acggacaact ggcgcaatta
    16321 cagggtttac aagattactg gcgtaatgaa ctgatccctg cgctgatgcg tgcacaaaac
    16381 cgagaaacgg tgtcagcgga tgtcagccag tttgttgccg ggcttgatca gctggtatct
    16441 ggttttgacc gcaccacgga aatgcgtatt gagacagtgg tactggtcca tcgggtaatg
    16501 gcggtattta tggcactttt actggtgttc actattatct ggttgcgggc gcgactgcta
    16561 caaccgtggc ggcaactgct ggcaatggcg agtgccgtca gccatcgcga ttttacccaa
    16621 cgcgcaaaca tcagcgggcg caacgaaatg gcgatgcttg gaactgcgtt gaacaatatg
    16681 tctgcagaac tggccgaaag ttatgccgta cttgagcagc gggttcagga gaaaaccgcc
    16741 gggctggagc ataaaaatca gatcctctct tttttatggc aggctaaccg ccgtttgcat
    16801 tcccgcgccc cgctgtgtga acgcctgtca ccggtactca acggcttaca gaatttaacc
    16861 ctgctacgtg atatcgaact gcgggtgtat gacactgatg atgaagagaa tcatcaggag
    16921 tttacctgcc agccagatat gacttgtgat gataaaggct gccagctctg cccgcgcggc
    16981 gtattacccg ttggtgatcg cggcacgacc ctgaagtggc ggctggctga ctctcatacg
    17041 cagtacggta ttttgctggc gaccctgccg caggggcgtc atcttagcca tgatcaacaa
    17101 caactggtgg ataccctggt tgaacaactt accgccacgc tggcgctgga tcgccatcag
    17161 gaacgtcagc aacagttgat cgtgatggaa gagcgtgcca ccattgcgcg cgaactgcat
    17221 gattctattg cccaatctct ctcttgcatg aagatgcagg tgagttgttt acagatgcag
    17281 ggcgatgcgc tgccagaaag cagccgcgaa ctgttaagtc agatccgtaa cgaactgaat
    17341 gcatcctggg cgcagttgcg tgaattgctc accacattcc gcttgcagct caccgagcct
    17401 ggattacgtc cggcgctgga agcgagttgc gaagagtaca gcgccaaatt tggcttcccg
    17461 gtgaagctgg attatcaatt gccgcctcgt ctggtgcctt cacatcaggc aatccacttg
    17521 ttgcaaattg cccgtgaggc attaagtaac gccctcaaac attcgcaagc gagtgaggtc
    17581 gtggtgacgg tggcgcaaaa cgataatcag gtcaaactga ccgtccagga taacggctgc
    17641 ggcgtgcctg aaaatgccat ccgcagcaat cactacggca tgataataat gcgcgaccgt
    17701 gcgcaaagtt tacgaggcga ttgccgcgtc cgccgtcgtg aatcaggtgg caccgaagtg
    17761 gtggtcacct ttattcccga aaaaactttc acagacgtcc aaggagatac ccatgagtaa
    17821 tcaggaaccg gctactatcc tgctgattga cgatcacccg atgctgcgaa ctggcgtaaa
    17881 acagcttatc agtatggcac cagatatcac cgtggttggc gaagcgagta atggcgaaca
    17941 gggtattgaa ctggcggaat ctcttgatcc cgatctgatc ctgttagatc tcaatatgcc
    18001 cggcatgaac ggtctggaaa cgctggataa actgcgcgaa aagtccctct cagggcgcat
    18061 tgtgatattc agcgtctcta accatgaaga agatgtggtc accgcactga aacgcggcgc
    18121 ggatggctat ctgttaaaag atatggaacc ggaagatctg ctgaaagcat tgcatcaggc
    18181 agctgctggc gaaatggtat taagcgaagc attaacgcct gttctggccg ccagcttgcg
    18241 cgctaaccgt gccactactg agcgcgatgt taaccagtta accccacgcg agcgcgatat
    18301 tctcaagctg attgcccagg gtttgccgaa caagatgatt gcccgccgcc tggatatcac
    18361 cgaaagcaca gtaaaagtgc acgtcaagca catgctgaag aaaatgaagc tcaagtctcg
    18421 cgtggaagca gcggtatggg tgcatcagga gcgcattttc tgattacggt tcccagcgca
    18481 gttcgtcgtt tgacaatgca tcgaacggtt cgacaagcgt tagcgtgatc tcattggagg
    18541 agacacgctg cccctggtta tcttccacca ccacagacaa tcgccagtga ttgcttgccc
    18601 cttccccgtt ctgccagtca ggcatgatca gcgtccagcc ctccgcgctg ttggcttgtg
    18661 cgcccggcgt caaacttaat atctgcgtat cgccctgcca aatcagttgc cgaataccgt
    18721 aacgactgcg catttgtaat ttcagcggca ctgtttcgcc aggttttaga tcccacggcg
    18781 gtgtcgccag aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc ggtagattat
    18841 ttcgctgcgg attgtcatag cgactaccac gtaacgactg actttcggca acctcgcccg
    18901 cagaaagttg ttttttgagc ggtacaccaa agcggtaatt aagattcagc ccgaggttat
    18961 tttgattttc gccactttcc ccctgtttat gctgggccgt cacagtgact aatggcacag
    19021 gggtgtaatt taatcccaga ctcaacgcga cgggattgtg ataacccgta ccaaagttaa
    19081 acaaatcaac acgatcacca aaatactgtt ctaggctgac actggtattg aggtgttgat
    19141 agaacggcat gcgcatccga gctgtcaggt cgtacccgcg cgccatccgt tgttcctgcg
    19201 tggctgtctg ttcatgccat gcagcaaacg gctgataaaa gtttgccgat aatcgcaaat
    19261 attcgcccca cgcttcggca ccaaagcccg ctcgctgaag attttcgtcc tgcaagttgt
    19321 cataaaaagt gttataaccc accagccagt tgccgcgcgc ccagcgttgc ccaacgccca
    19381 cattgctcac caacccatta tcctgctgag taagaccaag ctggctccag gtgagataac
    19441 gatcattatc ttgtaacggc acaaaccagc ttccacgact gccggtgaaa tgtccttcgt
    19501 tatccacttt gacgtcaaca ctggcatttc cccacggtga tagccaggac tctacgtgct
    19561 gattaacctg ttgactaagc gcgtcgcgga cttttcccaa tgcgaaagct tttgcctgct
    19621 cgccagtatc cagcccgtta tcattcatac tggtttcgcc gaaatctttc acaatttcag
    19681 caaagtgttt ttccccatca tgattttcgg gagccatgcc taaatccggc agaccatcat
    19741 tgttattatc aaagggattt gccgcttttt gctcgaaggt agattgtgcg ttagctgtac
    19801 cgcctgctac aagcaagagt aaataaaacg ggataatgcg aggaacgaaa cggctcaaaa
    19861 cgtcggtaac ttccggtaat aatgaaaaac catagcctta aagaataacg attactggca
    19921 acttttgtcg tattttctcg cgctttcagg aaaatcctta aaaatgtgac cgattggtca
    19981 gacgctgcag aagcagtaag atagaagatg agtgtctaat tccctgaata caggagtaaa
    20041 taatgaaaaa aatcgtgatc gttgccaatg gcgcacctta cgggagcgaa tccttgttta
    20101 acagcttgcg gctggccatt gcgttacgag agcaggagag caatctggat ctgcgtctgt
    20161 tcctgatgtc tgatgcggtc acagccgggt tgcgcgggca aaaaccaggg gaaggctaca
    20221 acattcagca aatgctggag atccttaccg ctcagaatgt accggtgaaa ttgtgcaaaa
    20281 cctgtaccga cgggcgcggg attagcacac ttcctctgat tgatggggtg gaaatcggta
    20341 ctcttgtgga actggcgcaa tggacgctgt cagccgataa agtgctcaca ttttaatgtt
    20401 taagggggcg tctgacgccc cactatgagg gaagtttact caggcgaatc ccggccttaa
    20461 cacaccatcc ggataattct ccgccagtag tttttttacc gataccagca aatcattcag
    20521 cccatcgtcc tgcattccca gtttgataag ctcctgttcc agtgaaaaca ggtattgtgc
    20581 attggttccc agcggaccgc tcgccgccgc aatcaacggc gcgatgacct gagcgcgagt
    20641 gtcagattca tattctggat gtcgcgggtc cataataaac acaatggcgt ttactgtgcg
    20701 tccatcatca agatcaagct gacaccaggt tggcagataa cagccggtaa tcatctcgcg
    20761 cttccacaac agggttagtt cctgctccag cgtctcttct ggtagtcgat aggcgacgcc
    20821 tgtggtgcgt ccgccctctt tcagtgcaag catccgtccc ggctggtgcg cagtcccgcg
    20881 cccggcggtc aggcgcaggc aaaacgcgcg atgccatcca accagtgtac cggtgcatga
    20941 ttcggtaaac tccagtgccg gattccacat caacgatcca taaccgaaga tccacaccgg
    21001 tccctcatca ggtcgacaag ccagcgtcgc cgccagcgaa gccgcccgtt gttctgctga
    21061 ccataagagt gattcttcaa tggcaccaaa tgccgtttta caatcggcat tcatcaagaa
    21121 atcacgcgtt atcaccttac acctccgcca ctgcatactt ccctgcgaca actttttggt
    21181 gtcttccaga catttttacc gctcattttg aaaccactaa cgggacaata tgcaatccac
    21241 ggaccacttg caaggcaaca ccgcacgaat agctttaact aagctaccgg ttttacgatt
    21301 ttttatgcca tttatcatca tcccctttgg catattcatg cttcacagca gcccaggcca
    21361 ctttatgcgc ggtttcttcg cgactggcgt catcacgccg atcttcttta tctttatatt
    21421 gatcccatgc gctgttgaac gcttctttat agatatcctg ggcatgagac ggtagaacgt
    21481 gctttacgct ttccggcaga tcgcttttcg ttttatacgg catcgttgac tccttttaca
    21541 aagttgaaca ttaagtgtgg tcaacaatat ctactcttgc caaaacataa gaattttcat
    21601 gtcagactgt tcttaagaaa cgctaaaaca aacattgtct aaacgtaata cttattttta
    21661 tgccccaatg agtaaaaaat cgacagcggg gtaaaaattc gtaaaatgtt accgatatcc
    21721 agacactttc tgttattttt cctacctatt ttttacattt tcgataaatt catctgcaaa
    21781 gggataacca taatgtcaaa tgctcaagag gcggtaaaaa cccgccacaa ggagacttcg
    21841 cttattttcc cggttctggc gctggtagtg ctgttcctgt ggggaagtag ccagacacta
    21901 ccagtggtca ttgccatcaa tcttcttgcg cttattggtt ttttaagtag cgcctttagt
    21961 gttgtccgtc atgcggacgt attagcccat cgcctgggag aaccttacgg ttcgcttatt
    22021 cttagccttt cagtggttat tcttgaagtc agtttgattt cagctttaat ggcaaccggc
    22081 gacgccgcgc caacgctaat gcgtgatacg ctctattcaa tcattatgat tgttaccggt
    22141 gggctggttg gcttttcatt attgttgggc ggtcgtaagt ttgccaccca atatatgaat
    22201 ctgtttggta tcaagcagta tttaattgcg ctgttccccc tggcgataat cgtactggta
    22261 tttccaatgg ctctgcctgc ggcgaatttt tcaaccggtc aggcgttact ggtagcatta
    22321 atttctgcgg caatgtatgg cgtatttttg ctgatccagg ccaaaacgca tcaaagtttg
    22381 tttgtctacg agcacgaaga tgacagtgat gacgacgacc cgcaccacgg taaaccgtct
    22441 gcccatagca gcctgtggca tgctatctgg ttgattatcc atctgattgc cgttattgca
    22501 gtcaccaaaa tgaacgccag ctcgctggag acattgctcg acagcatgaa tgcccctgtt
    22561 gcctttactg gcttcctggt ggcactgttg attctgtcgc cggaaggttt aggtgcgtta
    22621 aaagcagtgt tgaacaacca ggttcagcgc gcgatgaatc tgttctttgg ttcagtgtta
    22681 gcaacaattt cgcttaccgt acctgtcgtc acgctaattg cctttatgac gggtaacgaa
    22741 ttgcagtttg cacttggtgc gccagaaatg gtggtgatgg tggcctcttt agtgctgtgc
    22801 catatctcct tctccaccgg acgtactaac gtgctcaatg gcgcagcgca tctggcactg
    22861 tttgccgcct atttgatgac gatatttgcc tgacagttat atctcctccg atgacggaaa
    22921 tcgggagaga taagataaat tcctgcttat tgtatttctt catactacct ggacagctcg
    22981 ttaatcagaa ttgcgagcct gtaccacaac ctgcacgaaa ccactgcaac agctgttgaa
    23041 aagccggaat gtactcttta ttgcaaacat ttcagggatg aagtgaagat gaattgagac
    23101 gtagtatcca caccaagccg gaattatccg gtgaggcgca atgttgcggg ggctttatcc
    23161 ctggtggcat tggttgctgg aaagagaaaa cccccgcacg ttgagaggta aaacctgaca
    23221 acaaaacggg ggctaatctt gaaaccagac acttcaagaa tagccgcaaa atgttgtcat
    23281 tacaacaagg cggctatatg acgctcgcgc agtttgccat gatttt
//
LOCUS       DAJTRQ010000055        22785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000055 DAJTRQ010000000
VERSION     DAJTRQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..194)
                     /locus_tag="OO332_002215"
     CDS             complement(<1..194)
                     /locus_tag="OO332_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5942685.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGY"
     gene            complement(335..2368)
                     /gene="metG"
                     /locus_tag="OO332_002216"
     CDS             complement(335..2368)
                     /gene="metG"
                     /locus_tag="OO332_002216"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5942686.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2500..3609
                     /gene="apbC"
                     /locus_tag="OO332_002217"
     CDS             2500..3609
                     /gene="apbC"
                     /locus_tag="OO332_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5942687.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3871..4152
                     /locus_tag="OO332_002218"
     CDS             3871..4152
                     /locus_tag="OO332_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5942688.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4445..4987
                     /locus_tag="OO332_002219"
     CDS             4445..4987
                     /locus_tag="OO332_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5942689.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5068..5742
                     /locus_tag="OO332_002220"
     CDS             5068..5742
                     /locus_tag="OO332_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5942690.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5758..8238
                     /locus_tag="OO332_002221"
     CDS             5758..8238
                     /locus_tag="OO332_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5942691.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8254..9287
                     /locus_tag="OO332_002222"
                     /pseudo
     CDS             8254..9287
                     /locus_tag="OO332_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="OO332_002223"
     CDS             complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="OO332_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5942692.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9926..10768)
                     /gene="rcnA"
                     /locus_tag="OO332_002224"
     CDS             complement(9926..10768)
                     /gene="rcnA"
                     /locus_tag="OO332_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR5942693.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10889..11161
                     /gene="rcnR"
                     /locus_tag="OO332_002225"
     CDS             10889..11161
                     /gene="rcnR"
                     /locus_tag="OO332_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5942694.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11253..11349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11384..12172
                     /gene="thiM"
                     /locus_tag="OO332_002226"
     CDS             11384..12172
                     /gene="thiM"
                     /locus_tag="OO332_002226"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5942695.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12169..12969
                     /gene="thiD"
                     /locus_tag="OO332_002227"
     CDS             12169..12969
                     /gene="thiD"
                     /locus_tag="OO332_002227"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5942696.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13034..13852
                     /locus_tag="OO332_002228"
     CDS             13034..13852
                     /locus_tag="OO332_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5942697.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13904..14650
                     /locus_tag="OO332_002229"
     CDS             13904..14650
                     /locus_tag="OO332_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5942698.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14624..15589)
                     /locus_tag="OO332_002230"
     CDS             complement(14624..15589)
                     /locus_tag="OO332_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5942699.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15586..16589)
                     /locus_tag="OO332_002231"
                     /pseudo
     CDS             complement(15586..16589)
                     /locus_tag="OO332_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16586..17062)
                     /locus_tag="OO332_002232"
                     /pseudo
     CDS             complement(16586..17062)
                     /locus_tag="OO332_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17216..18268
                     /gene="fbaB"
                     /locus_tag="OO332_002233"
     CDS             17216..18268
                     /gene="fbaB"
                     /locus_tag="OO332_002233"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5942700.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18576..19430
                     /gene="gatY"
                     /locus_tag="OO332_002234"
     CDS             18576..19430
                     /gene="gatY"
                     /locus_tag="OO332_002234"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5942701.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19459..20726
                     /gene="gatZ"
                     /locus_tag="OO332_002235"
                     /pseudo
     CDS             19459..20726
                     /gene="gatZ"
                     /locus_tag="OO332_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20736..21188
                     /gene="gatA"
                     /locus_tag="OO332_002236"
     CDS             20736..21188
                     /gene="gatA"
                     /locus_tag="OO332_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5942702.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21219..21503
                     /gene="gatB"
                     /locus_tag="OO332_002237"
     CDS             21219..21503
                     /gene="gatB"
                     /locus_tag="OO332_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5942703.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21507..22785
                     /locus_tag="OO332_002238"
                     /pseudo
     CDS             21507..22785
                     /locus_tag="OO332_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5905 a         5292 c         5752 g         5836 t
ORIGIN      
        1 tcatacccct tcttcacctt ctccgcaatc agcttcagtt ccgctttttc cgccgccgca
       61 gcatctgaaa aactttttat ctgactttgc cctttggtgc caacttttcc ccaactgata
      121 tgcaactcgt ttccctgttg ctccaccgtc cagaatttat gtgatttttc gtcctgatag
      181 ataaagtgtc tcattctgca tccctgtaat tttatccagt tgaatgcaga tgctaccagt
      241 atttatacgg gttagagaga gacaaaatcg caagaaaaca actttaggaa tttataaaga
      301 aaaggcgctg tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag
      361 caccggcatc cgggcttagc aggaaaatat ctttcccgcc aggaccggca gccatcacca
      421 tgccttcaga gataccgaag cgcattttac gtggtgccag gttagccacc ataatggtgt
      481 gacgaccaat cagtgcctgc ggatccgggt aagcagaacg aataccggag aagacattgc
      541 gtttttcacc gccgagatcc agcgtcaggc gcagcagttt gtcagaacct tcaacaaact
      601 ctgcgttttc gatcagcgcc acgcgcaggt caactttggc gaagtcgtca aaggtgatgg
      661 tttcctgaat cggatcatct gccagtgggc cagtaactgg cgcggtagtg gctttcactt
      721 cttctttaga ggcttccacc agagcttcaa cctgtttcat atcgatgcgg ttatacagcg
      781 ccttgaacgg attcactttg tggcccagca gcggttgctg gataccatcc caggtcagtt
      841 ccgtattgag gaaggcttct gcacgctcgg tcagtttcgg cagtaccggc ttcaggtaag
      901 tcatcagcac gcggaacagg ttgatgccca ttgagcaaat cgcctgcagg tcagcatcgc
      961 gaccttcctg ttttgcgact acccacggag cctgctcgtc gacatagcgg ttagccaggt
     1021 cagccagtgc catgatttcg cgcactgctt taccaaattc acggctttcc cacgcttcac
     1081 caatcacttc agcggcatcg gtaaaggttt tgtacagctc agggtcagcc agctcgcttg
     1141 ccagcacacc gtcaaaacgc ttgttgataa agcccgcatt acgggaggcc aggttaacca
     1201 ctttgttcac gatatcggca ttcacacgct gaacgaaatc ttccaggttg agatcgatat
     1261 catcaatgcg cgaagagagt ttcgcagtgt agtagtaacg caggctgtca gcgtcaaaat
     1321 gattcagcca ggtgctggct ttaataaagg tgccgcgaga cttggacatc ttcgcgccgt
     1381 tcaccgtcac atagccatga acaaacaggt tggtcggctt gcggaagttg ctgccttcca
     1441 gcatggcagg ccagaacagg ctgtggaagt aaacgatatc tttaccgatg aagtggtaca
     1501 gctcggcggt ggagtctttc ttccagtatt catcgaagct tacgctgtcg ccgcgcttgt
     1561 cgcacagatt cttgaaagaa cccatgtagc caatcggtgc gtccagccag acgtagaaat
     1621 atttgcccgg cgcgttcgga atttcaaaac caaagtatgg cgcgtcgcgg gagatatccc
     1681 actgttgcag gccggattca aaccactcct gcattttgtt tgccacctgc tcctgcaacg
     1741 caccgctgcg ggtccatgcc tgcagcattt cgctgaaaga gggcagatca aagaagaagt
     1801 gttcagaatc acgcatcact ggtgtagcac cagaaaccac cgatttcggc tcgatcaatt
     1861 cagtcgggct gtaggtcgcg ccgcagactt cgcagttatc gccgtattga tccggggatt
     1921 tacatttcgg gcaggtgcct ttcacaaaac ggtccggcag gaacatgcct ttttccggat
     1981 cgtacagctg agagatggtg cggtttttaa taaaaccgtt ttctttcagg cgagagtaga
     2041 taagttctga caactggcgg ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga
     2101 tgttaaagcc tgcgaaatca gtctgatgct cctgactcat ttcgccaatc atctgctccg
     2161 gggtgatacc gagctgctga gctttcagca tgatcggtgt accgtgggca tcgtcggcgc
     2221 agatgaagtt gacctcgtgg ccgcgcattc gctggtaacg gacccagaca tcagcctgga
     2281 tgtgctccag catatggccg aggtggattg agccgttagc gtacggcagt gcgcacgtca
     2341 ccagaatttt cttcgcgact tgagtcatag taggcattac ttcttaatag tgaaaagggg
     2401 ttagatagta ccaaatggga aaatgttaag taagtacttc tctatattct tcaggtaaac
     2461 tatcagctga tggtttatct atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc
     2521 aaatcaccag aagccttgcg cgcaatggtc gccgggacac tggccaattt tcagcaccca
     2581 accctgaagc ataacctcac cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac
     2641 acgttgcatg tggaactggt tatgccgttt gtctggcata gcgcgttcga agagttaaaa
     2701 gagcaatgta gcgccgaact gctgcgcatc accggggcga aagctatcga ctggaaactg
     2761 tcgcataaca tagccactct gaaacgcgtg aagaatcagc cgggaattaa cggtgtgaag
     2821 aacattatcg cggtgagttc cggtaaaggc ggcgtgggta aatcgtccac ggcggtaaac
     2881 ctggcgctgg cactggcggc ggaaggggcg aaagtcggca ttctggacgc cgatatctac
     2941 ggcccatcaa ttcccaccat gctgggcgca gaaaatcagc gtccaacttc gccggacggt
     3001 acccacatgg caccgattat gtctcatggc ctggcaacca attcaattgg ttatctggtc
     3061 actgacgaca atgcgatggt gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg
     3121 ttgcaggaaa ccttgtggcc ggatctcgac tatctggtgc ttgatatgcc gccaggcacc
     3181 ggtgacattc aattgacgct ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc
     3241 accccgcagg acatcgcgtt gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc
     3301 gaggttccgg tgctcggtat tgtcgagaac atgagtgtgc atatttgcag taactgtggt
     3361 caccatgaac cgattttcgg taccggtggt gcagagaaac tggctgaaaa ataccataca
     3421 cagctgctgg ggcagatgcc gttacacatc tccttacgtg aggatctcga taaaggcacg
     3481 ccaacggtta tcagccgtcc ggagagcgaa tttaccgcta tctaccgcca gctggctgac
     3541 cgcgttgcag ctcagctcta ctggcagggt gaagtcattc ctggcgagat ttccttccgc
     3601 gcggtctaac gcctattaac cattctggtt attcttcata catcgcagag atgttatatt
     3661 gatgcggtgt atttgttttt gccgcattaa tgaacactta tttaatactg cgaatattct
     3721 gcggaccttc gtccgaaact ctacaaagtc gagtgatcgt acaggcaatg cttcattatc
     3781 atccttcgtg ctgtgtagtg gatgtacagc tatagtttgt tatgcactca ttctatccag
     3841 gcataacctt tactacaggg ataacgttca atgaataagt attggctgtc aggaattatt
     3901 tttcttgcct atggattggc ttctcccgca gtctcttcag agactgccac attaacgatt
     3961 aatggcagga tcagtccccc aacatgcagt atggctatgg ttaatagtca accacaacag
     4021 cattgtggtc agttaacgta taatgttgac acccgtcatc aggtttcttc gcccgtcaaa
     4081 ggtgttacga cagaagtggt tgttgcaggt agcgatagca aacgtcgaat cgtgttaaat
     4141 cgctacgatt aatttggcaa cctgatttta attgcattta gaaagacgaa atcatttata
     4201 taaatgattt cgtctttttt attgatatca acaataccat ttacataaat ctacattaat
     4261 taatagatgt gataaatatt taatattctt aagtatttgt tgtatttata ttgcattggt
     4321 taatttgttt taaatcaata aaacaatatg tttacattgc ggcagctttt ttgatgtgag
     4381 aaatttgttt ccaagagata cagactctta acaaccaaaa ataaatttta ttaaggaaca
     4441 tattatgaaa cgttcaatta ttgctgccgc tgtcttttct tcttttttta tgagcgctgg
     4501 agtatttgct gcagacgttg ataccggaac attaaccatt aaaggaaata ttgcagaatc
     4561 tccgtgtaaa ttcgaagcgg gtggtgattc agtaagtatt aatatgccga ctgtaccaac
     4621 cactgtcttt gaaggtaaag ctaaatattc tacctatgat gatgcagtcg gtgtaaccag
     4681 cagcatgtta aaaattagct gcccgaaaga agttgctggt gtaaaactct cgttgatcac
     4741 aaacgacaaa ataaccggta acgataaggc gatagccagt agcaacgata ccgtgggtta
     4801 ctatctctat ttaggtgata acagcgatgt cctagatgtt tctgcacctt ttaacattga
     4861 gagttataaa acagcagaag gtcaatatgc tattccgttt aaagcaaaat acctgaaact
     4921 gacagataac tcagtgcaat caggtgatgt gttatcttct ctggttatgc gtgtagcgca
     4981 ggattaataa ataaaattgc aagtttctcc aggtaataaa catggccgct atccccatgg
     5041 cggcttttta atttaagagg cattaagatg aaaggattat tatctttact cattttttct
     5101 atggtccttc ctgcacatgc cggaattgtt atctacggga cgcgcattat ttacccggca
     5161 gaaaataaag aagttatggt gcagttgatg aaccagggaa atcgttcttc gctgctgcag
     5221 gcgtggattg atgatggcga tacgtcgtta ccaccagaaa aaattcaggt tcctttcatg
     5281 ttaacgccac cagtggcaaa aataggggca aattcagggc agcaagtaaa aatcaaaatt
     5341 atgccgaata aactgcccac taataaagaa agcatttttt atctgaatgt tctggatatt
     5401 ccaccaaata gtccagagca agaaggcaag aatgcactga agtttgcgat gcaaaacaga
     5461 attaagttgt tttaccggcc agcgggtatt gctccggtaa ataaagcgac ctttaaaaag
     5521 ttactggtaa atcgcagtgg caatggattg gtgataaaaa atgactcagc taattgggtg
     5581 acgatttcgg atgtcaaagc taataatgtt aaagtcaatt atgaaactat tatgattgcc
     5641 cccttagaaa gtcagagtgt taatatcaaa agtaataatg caaataactg gtatctgacc
     5701 attatcaatg accacggcaa ctatattagt gacaaaattt aattgcagga gctgcctatg
     5761 ttgagaatga ccccacttgc atcagcaatc gtagcgttat tgctcggcat tgaagcttat
     5821 gcagctgaag aaacctttga tacccatttt atgataggtg gaatgaaaga ccagcaggtt
     5881 gcaaatattc gtcttgatga taatcaaccc ttaccggggc agtatgacat cgatatttat
     5941 gtcaataagc aatggcgcgg gaaatatgag attattgtta aagacaaccc gcaagaaaca
     6001 tgtttatcaa gagaagttat caagcggtta ggcattaata gcgataactt cgccagcggt
     6061 aagcaatgtt taacatttga gcaacttgtt cagggtggga gctatagctg ggatatcggg
     6121 gtttttcgtc tcgatttcag tgtcccgcag gcttgggtgg aagaactgga aagtggctat
     6181 gttccaccgg aaaactggga gcggggtatt aatgcgtttt atacttctta ttatgtgagt
     6241 cagtattaca gcgactataa agcgtcgggt aacaacaaga gtacatatgt acgttttaac
     6301 agcgggttaa atttactgga gtggcaactg cattctgatg ccagtttcag taaaacaaat
     6361 aacaatccag gggtgtggaa aagcaatacc ctgtatctgg aacgtggatt tgcccaattt
     6421 ctcggcacgc ttcgcgtggg tgatatgtac acatcaagcg atatttttga ttctgttcgc
     6481 ttcagcggtg tgcggttgtt tcgtgatatg cagatgttgc ctaactcgaa acaaaatttt
     6541 acgccacggg tgcaggggat tgctcagagt aacgcgttgg taactattga acagaatggt
     6601 tttgtggttt atcagaaaga ggttcctcct ggcccgtttg cgattacaga tttgcagttg
     6661 gccggtggtg gagcagatct tgatgttagc gtgaaagagg cggacggctc ggtaaccacc
     6721 tatctggtgc cttatgcagc ggtgccaaat atgctgcaac ccggcgtgtc gaaatatgat
     6781 tttgcggcgg gccgtagcca tattgaaggg gcgagcaagc aaagtgattt tgtccaggca
     6841 ggttatcagt atggttttaa taacttattg acgctgtatg gcggcacgat ggttgctaat
     6901 aattactatg cgtttaccct cggaacgggt tggaacacgc gcattggcgc aatttccgtc
     6961 gatgccacga agtcgcatag taaacaagac aacggcgatg tgtttgacgg gcaaagttat
     7021 caaattgcct acaacaaatt tgtgagccaa acgtcgacgc gttttggtct ggcggcctgg
     7081 cgttattcgt cgcgtgatta ccggacattt aacgatcacg tttgggcaaa caataaagat
     7141 aattatcgcc gtgatgaaaa cgatatctat gacattgccg attattacca gaacgatttt
     7201 ggccgtaaaa atagcttttc tgccaatatg agccagtcat tgccagaagg ctggggttct
     7261 gtgtccttaa gtacgttatg gcgagattac tgggggcgta gcggcagcag taaggattat
     7321 cagttgagtt attccaataa ctggcggcgg ataagctata ccctcgcggc aagccaggcg
     7381 tatggcgaga atcatcatga agagaaacgt tttaatattt ttatatcaat tccctgtgat
     7441 tggggtgatg acgttacgac gcctcgtcgg caaatatata tgtctaactc aacgacgttt
     7501 gatgatcagg gttttgcctc aaataatacg ggattatcag gaacagtagg gagtcgggat
     7561 cagttcaatt atggtgtcaa cctgagtcat caacatcagg gaaatgaaac gacagctggg
     7621 gcgaatttga cctggaacgc gccggttgcg acagtgaatg gcagttatag tcagtcgagt
     7681 acttatcgac agactggagc cagtgtttca gggggcattg tcgcctggtc gggtggcgtt
     7741 aatctggcga accgtctttc cgaaacgttt gctgtgatga atgcgccagg aattaaagat
     7801 gcttatgtca atgggcaaaa atatcgcaca acaaaccgta atggagtggt ggtatacgac
     7861 ggaatgacac cttatcggga aaatcacctg atgctggatg tgtcgcaaag cgatagcgaa
     7921 gcagaattac gtggcaaccg gaaaattgcc gccccttatc gcggcgcggt tgtactggtt
     7981 aattttgata ccgatcagcg caagccctgg tttataaaag cgttaagagc ggatggtcaa
     8041 ccattaacgt ttggttatga agtcaatgat atccatggtc ataatattgg cgttgtcggc
     8101 cagggaagcc agttatttat tcgcaccaat gaaataccgc catcggttaa tgtagcaatt
     8161 gataagcaac aaggactttc atgcacaatc accttcggta aagagattga tgaaagtaga
     8221 aattatattt gccagtaata gtcaggtggt tctatggaaa ttcgcataat gctatttata
     8281 ttaatgatga tggttatgcc tgtgagctat gcggcatgtt atagtgagtt atctgttcag
     8341 cacaatttgg ttgttcaggg gaattttgca cttactcaaa cacaaatagc gacatatgag
     8401 cataatttta atgattcgtc atgcgtgagt acaaatacta tcacccctat gagcccatcg
     8461 gatattattg ttggacttta taacgatacc attaaattaa atttacattt tgaatggacc
     8521 aataaaaaca acatcacgtt gtcaaataat cagagcagtt tcaccagtgg ttattcggta
     8581 acggtgacac ctgcggccag taatgcaaaa gtgaatattt ctgcgggggg cggtggttca
     8641 gtgatgatta atggtgttgc gacattatcc agtgcttcat catcgacacg cgggagtgcc
     8701 gcagtacaat ttctactgtg tttattaggt ggcaagtcat gggatgcatg tgtaaatagc
     8761 tacagaaatg cattggcaca aaatgcaggt gtctattcct tcaatctgac attgtcatac
     8821 aacccgataa ccacaacctg caaacaggac gatttattaa ttactttaga cagtattccc
     8881 gtttcacaat taccagccac aggtaacaaa gcaacaataa atagtaaaaa aagggatatt
     8941 attctgcgtt gtaaaaattt attaggtcaa caaaatcaaa catcacggaa aatgcaggtg
     9001 tatttatcaa gttctgactt gttaaccaac agcgacacaa tactgaaagg tgcggaagat
     9061 aatgcgtagg atttattctt gaaagtaatg gttcgccagt cacactttta aatatcacta
     9121 acagcagtaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa cttacagata
     9181 caacggtttc aattccgata acagccagtt actacgtcta cgatacaaac aaagttaaat
     9241 ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa caagcaagac
     9301 gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc atgatgaggg
     9361 ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat tttaccgtcg
     9421 gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc ggcatcaggc
     9481 gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg gtacatctct
     9541 gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact taactcatac
     9601 ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca ggcggcactg
     9661 gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa ctctccactt
     9721 tggggatctg taatatcagt cgcatatctt tcctgccata caatataact gacaagcgat
     9781 attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc cctaaaagca
     9841 tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct gggctgaaga
     9901 taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca tgtacgccca
     9961 tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt ttagcgagag
    10021 tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg ccaacgccta
    10081 cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt gcgcccagtg
    10141 tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg caggggataa
    10201 ggccaccggt taagccaaat aacaaaattt gccagttgat gacctctcta ccatcaaagc
    10261 gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg ccatgttcgt
    10321 gatggtgatg atgtccttgg tcgtggtgat gttcgtggtc ttgatgatgt tcgtgatgat
    10381 ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt tcgccgcgcc
    10441 aggtacgcca gaacatccag aacacggtgc caataatgat cactgcggaa atcagctgga
    10501 gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg ccaaaggcaa
    10561 tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt
    10621 taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt
    10681 ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt
    10741 gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct
    10801 gattctactc ccccccagta cctgatacta ccccccagta gattaatagt gctatgattt
    10861 ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact
    10921 gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga
    10981 gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggag cggtaaacgg
    11041 tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga
    11101 gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata
    11161 atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgggt gcgctggaca
    11221 ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt
    11281 gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg
    11341 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct
    11401 gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
    11461 ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat accttgctgg cgctcggtgc
    11521 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc
    11581 cttgttgatt aacgttggca cactgacgca gccacgcgca caggcgatgc gtgctgccgt
    11641 tgagcaagca aaaagctctc aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct
    11701 cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg
    11761 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggcggacggg gagtggatac
    11821 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
    11881 aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggacatcgta tcattggtat
    11941 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
    12001 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
    12061 ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt
    12121 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
    12181 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt
    12241 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
    12301 aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc
    12361 gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc
    12421 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc
    12481 gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg
    12541 ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc
    12601 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
    12661 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
    12721 agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
    12781 accaaaaaca cccacggcac tggttgtaca ctctctgcgg cgttggctgc actacgcccg
    12841 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta
    12901 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
    12961 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
    13021 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg
    13081 cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta
    13141 ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc
    13201 tttcgctatt cgcattcctg ccagttacac cattcacgga ttagatgttt cgcgctggca
    13261 ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt
    13321 tgcttttatt aaggctacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
    13381 gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct ccccgtcggt
    13441 atccgcttca gttcaggcgc gattatttct gcaaacggtg gatttctcac aaggcgattt
    13501 ccctgccgtg ctggacgtag aagaacgggg aaaattatcg gcaaaagaat tacgcaagcg
    13561 ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga ttatttactc
    13621 aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc cgtggtgggt
    13681 ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
    13741 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
    13801 cacggtggat gagttgcagg cattcgttga tgggattaaa gaaacgcctt aaataagaag
    13861 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
    13921 ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc cgctttatat
    13981 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
    14041 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
    14101 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
    14161 gatcaacaac atcttcgaat actcgttgaa agaagcgaga ggtttttctc agcaggtggt
    14221 attgcgcggg aaaaagcccg atacgctatg ggttaataag cgtgtcgtga aatgtcccga
    14281 agaagttgcg cagcagctgg cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat
    14341 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
    14401 tcatgatgtt gatgccatcg gaatttctct ttatgactat ttccgcagcc agcacattta
    14461 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
    14521 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
    14581 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
    14641 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
    14701 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
    14761 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
    14821 atgcccgaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
    14881 gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt gttatctctg
    14941 cagataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt gacactaaag
    15001 gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata cgtgggccaa
    15061 aatcgataaa cggcgtcacg tcttgcagct tttccagcca ttccactaac aattcgccgc
    15121 agggcgaggc cagttgataa ccggaaaaat agagcaggtt gccaggcgca acggttaatc
    15181 gtgccaacca ttggcgattc cattgatttt ccacaccgct gaatgacata aaagtgcgtt
    15241 cgccatccgg ctcaaccagt gccagacacc agccgttatc gctttcggcg ttatcgatca
    15301 ggctgattaa cccctctttc gccatccggt tgcgaataat ctccgcccac acgccctgac
    15361 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
    15421 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
    15481 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
    15541 gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg
    15601 ttgttgacga tatttctcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
    15661 atccagctcc gcctttaacg caggatcgac agcgttaatg ccatgcaacg cgccgcaaat
    15721 cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca agattggcgc acaggacggc
    15781 gcagcggttt gggtcggttt gtgccagttc aactagcgca atggcgcacg gaacggactc
    15841 aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
    15901 gtcggcatta cgcacaattt tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa
    15961 cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc
    16021 tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
    16081 aaccgccaga tcggatttat gggtcgggct ggacgccagc gccacatcat caataaagga
    16141 atcaacatca cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
    16201 ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
    16261 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc
    16321 gagaatatta cgcccaatca gattcggatc gatcttgcct tcacattcca gtaacgcatc
    16381 cgccagacac aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc
    16441 gcgttattct cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg
    16501 cggctgcgtg gccaaagctc ggagggcatc cccatcgcat cccctaacgc ctgcccataa
    16561 agagcaccga gaatacgttc tgttttcatt taacttcccc ttgtgtcaac gcaatatctc
    16621 gatcatcgac cttgatagcc gtaatttcgt tgtcggattc gcgggaaaaa atcatgaaca
    16681 gcacggcgat aatggcaatc atcaccgcgc cgaaagtcca catcccggac cagttgaaag
    16741 tcagtccgtt taccggttcc tgataagcga acatcttttc cgtcatcaca ccgccaagac
    16801 gatagccgag caaactgccg aagccctggc agcagagcgt gatcagcccc tgcgcagcgg
    16861 tacgcatatg cacgggggct tttttatcga catagatgta agcggtaacg tagtaaaaat
    16921 cgtaacttac gccgtgaagc aaaataccga ggaacagtaa cgcgtaggtg aaattgacag
    16981 gtaatgtaag gtacgcgatg acaataccac ataataatac cttttgaata cctttgcggt
    17041 aaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa
    17101 aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag
    17161 gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac
    17221 agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac
    17281 tattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga
    17341 caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct
    17401 ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg
    17461 agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat
    17521 cgaagcgggc tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg
    17581 ctatgcgcat cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc
    17641 gaatacctac gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt
    17701 ggcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat
    17761 ttctgcggcc tttgaacgcg cgcacgagct gggcatggtg gcagtgctgt gggcctattt
    17821 gcgaaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg
    17881 tcaggcgaac catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga
    17941 aaataacggc ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa
    18001 gttaaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg
    18061 ccgggctggg ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc
    18121 agtgcgtact gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa
    18181 ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta
    18241 tctcgatagc aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg
    18301 acggataagg cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct
    18361 tatcatgcct acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg
    18421 cactttgcta cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat
    18481 tttcatattg tcgtttttgt gatcgttatc tcgacattta aaagcaaata atttcattat
    18541 tttttgaaat cgaaagcaaa caacaagata tgaaaatgta cgtggtatcg acaaagcaga
    18601 tgctgaacaa cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg
    18661 aaacgatgca agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg
    18721 gaacgcctgg cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga
    18781 tggcgaagca atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg
    18841 atatcgctca gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc
    18901 cttttgcgca aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg
    18961 tcagcgtcga ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg
    19021 aagccgatgc gttgtacacc aaccccgcac aggcgcgtga atttgccgag gcaaccggga
    19081 ttgattccct ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc
    19141 ttgatttttc tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg
    19201 gcgcgtcagg attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa
    19261 tcaacgttgc aacggagctg aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg
    19321 agcaccccga agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg
    19381 atgtggtgag caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc
    19441 atttaacaag gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc
    19501 ggcatatgtt cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat
    19561 cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc
    19621 ggttataccg gaatgacact ggcagacttt cgcgaatttg tttttacgat tgccgataaa
    19681 gttgggtttg cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg
    19741 cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt
    19801 cgtgctggct tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata
    19861 ccgttagcac cagaaacggt tgcggaacga gctgctgtgc tttgctttgc tttgctgcgg
    19921 aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc actgaagttc
    19981 cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat gttgaagatg
    20041 ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg acagaggcgt
    20101 taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac agcaatatta
    20161 tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac acccgaatgg
    20221 tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa ttagtccgcg
    20281 atcactttgc aatattgaaa gtcggtcctg cattaacctt tgctttacgc gaggcgatat
    20341 ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc ggttgcctgg
    20401 cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg
    20461 gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt cgttattact
    20521 ggccgcatag tcggattaaa aatagcgttg aaacgatgat ggtgaatctg caaggcgtgg
    20581 acatcccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc attcagtccg
    20641 gggaattatc agcaatgccg catcagctta ttatgaataa aatttatgat gttttgcgcg
    20701 cctatcgcta cggttgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg
    20761 gaatttcttt tgtcgatcgt agcgaagttt taacctatat cggtaatgag atgctcgcga
    20821 aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca gaattcccta
    20881 ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta
    20941 agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg
    21001 atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc
    21061 aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac
    21121 taattactct tcctgaaact cagttaaagg aatacttcac aaagtatgtt ttagattcag
    21181 acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag attattgtcg
    21241 cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc
    21301 agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata
    21361 tggatggtgt gcatttgata tgtaccactg ccagggtgga tcgtagtttt ggcgatattc
    21421 cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa
    21481 ttctgactat cttacagggg tgacctatgt tttcagaagt catgcgttat attctcgacc
    21541 tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata ttaggcatga
    21601 aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt ggcattggcc
    21661 ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg gcggaaaatt
    21721 tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca atgacctgga
    21781 cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg gcgatgttac
    21841 tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca
    21901 ccggcagcgt tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta
    21961 attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc
    22021 tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg
    22081 gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc
    22141 gacgatattc agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg
    22201 gggctgatta tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta
    22261 aaaacggcgg cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta
    22321 acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc
    22381 ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg
    22441 atttttattc cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg
    22501 tttggcgatc ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga
    22561 aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg
    22621 acgcaaacta ttggtttgca cacccaactg gcggctaatg ctggggcgtt aaaagccggg
    22681 ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt
    22741 ttctccccgc aaaatattcc cggtttcatt attatcggcg caatt
//
LOCUS       DAJTRQ010000056        22074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000056 DAJTRQ010000000
VERSION     DAJTRQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            108..766
                     /gene="csiR"
                     /locus_tag="OO332_002239"
                     /pseudo
     CDS             108..766
                     /gene="csiR"
                     /locus_tag="OO332_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(767..1216)
                     /gene="kbp"
                     /locus_tag="OO332_002240"
     CDS             complement(767..1216)
                     /gene="kbp"
                     /locus_tag="OO332_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5942704.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1300..1458)
                     /locus_tag="OO332_002241"
     CDS             complement(1300..1458)
                     /locus_tag="OO332_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5942705.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            1641..1940
                     /locus_tag="OO332_002242"
     CDS             1641..1940
                     /locus_tag="OO332_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5942706.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            1950..2474
                     /gene="ygaP"
                     /locus_tag="OO332_002243"
     CDS             1950..2474
                     /gene="ygaP"
                     /locus_tag="OO332_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5942707.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(2521..2925)
                     /gene="stpA"
                     /locus_tag="OO332_002244"
     CDS             complement(2521..2925)
                     /gene="stpA"
                     /locus_tag="OO332_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5942708.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            3593..4030
                     /gene="alaE"
                     /locus_tag="OO332_002245"
     CDS             3593..4030
                     /gene="alaE"
                     /locus_tag="OO332_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5942709.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4067..4417)
                     /locus_tag="OO332_002246"
     CDS             complement(4067..4417)
                     /locus_tag="OO332_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5942710.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            4569..4898
                     /locus_tag="OO332_002247"
     CDS             4569..4898
                     /locus_tag="OO332_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5942711.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            5146..5391
                     /gene="nrdH"
                     /locus_tag="OO332_002248"
     CDS             5146..5391
                     /gene="nrdH"
                     /locus_tag="OO332_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5942712.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5388..5798
                     /gene="nrdI"
                     /locus_tag="OO332_002249"
     CDS             5388..5798
                     /gene="nrdI"
                     /locus_tag="OO332_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5942713.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            5771..7915
                     /gene="nrdE"
                     /locus_tag="OO332_002250"
     CDS             5771..7915
                     /gene="nrdE"
                     /locus_tag="OO332_002250"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5942714.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            7925..8884
                     /gene="nrdF"
                     /locus_tag="OO332_002251"
     CDS             7925..8884
                     /gene="nrdF"
                     /locus_tag="OO332_002251"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5942715.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9239..10441
                     /gene="proV"
                     /locus_tag="OO332_002252"
     CDS             9239..10441
                     /gene="proV"
                     /locus_tag="OO332_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5942716.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10434..11498
                     /gene="proW"
                     /locus_tag="OO332_002253"
     CDS             10434..11498
                     /gene="proW"
                     /locus_tag="OO332_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5942717.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            11556..12548
                     /gene="proX"
                     /locus_tag="OO332_002254"
     CDS             11556..12548
                     /gene="proX"
                     /locus_tag="OO332_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5942718.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            12740..13919
                     /locus_tag="OO332_002255"
                     /pseudo
     CDS             12740..13919
                     /locus_tag="OO332_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14043..14780
                     /gene="ygaZ"
                     /locus_tag="OO332_002256"
     CDS             14043..14780
                     /gene="ygaZ"
                     /locus_tag="OO332_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5942719.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            14770..15105
                     /gene="ygaH"
                     /locus_tag="OO332_002257"
     CDS             14770..15105
                     /gene="ygaH"
                     /locus_tag="OO332_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5942720.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15196..15726
                     /gene="emrR"
                     /locus_tag="OO332_002258"
     CDS             15196..15726
                     /gene="emrR"
                     /locus_tag="OO332_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5942721.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            15853..17025
                     /gene="emrA"
                     /locus_tag="OO332_002259"
                     /pseudo
     CDS             15853..17025
                     /gene="emrA"
                     /locus_tag="OO332_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17042..18580
                     /gene="emrB"
                     /locus_tag="OO332_002260"
     CDS             17042..18580
                     /gene="emrB"
                     /locus_tag="OO332_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5942722.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(18644..19159)
                     /gene="luxS"
                     /locus_tag="OO332_002261"
     CDS             complement(18644..19159)
                     /gene="luxS"
                     /locus_tag="OO332_002261"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5942723.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19309..20865)
                     /gene="gshA"
                     /locus_tag="OO332_002262"
     CDS             complement(19309..20865)
                     /gene="gshA"
                     /locus_tag="OO332_002262"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5942724.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(20938..21366)
                     /locus_tag="OO332_002263"
                     /pseudo
     CDS             complement(20938..21366)
                     /locus_tag="OO332_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21363..21929)
                     /gene="yqaB"
                     /locus_tag="OO332_002264"
     CDS             complement(21363..21929)
                     /gene="yqaB"
                     /locus_tag="OO332_002264"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5942725.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5327 a         5623 c         5532 g         5592 t
ORIGIN      
        1 atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa
       61 aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt
      121 ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg
      181 aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg ccgttgcggg
      241 aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc
      301 gggtggcgtc tatgtcagag caggagctgc tctgacatag acgccacccg atatggaagc
      361 gatgctggtc agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc
      421 aaaagcgcat ctgctcaata agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga
      481 gtgggatctg cgtcatcagg cgtttcatac agcaattgtg gcgggctgtg gttctcacta
      541 tttgctgcaa atgcgtgaac ggttgtttga tctggcggcg cgttatcgat ttatctggct
      601 gcggcgaacg gtgctttcgg tggaaatgct ggaggataaa cacgatcagc accagaccct
      661 gactgcggcg gtactggcgc gagatacagc gcgcgtcagt gagttaatgc gccagcattt
      721 actgacgcca attcccatta tccagcaggc gatggctggc aattaactac tcttccggaa
      781 tacgcaacac ttgccccgga taaattttat ccgggctttt tagcatcggt ttattcgctt
      841 cgaagatttt attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag
      901 tgtcgccaga cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct
      961 gatcatcaac actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg
     1021 cctcctgact caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca
     1081 ctttatcggc atcaggtata ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat
     1141 cgtctttatc gtgctgacct gtaaccgcat cccagagttt ttctccggca tctttcacaa
     1201 aattgaacag acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga
     1261 ggaacgcggc gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg tttgcaccca
     1321 gaacgcgtga atcaaaccgg ggatataccc aagcagtgtc agcagaatat taataatgaa
     1381 cgcccaaccg aaccctttac cgagcagcac gccgagcggc ggcagaatga tggtgatgac
     1441 gattctccag aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga
     1501 ttttatctct taagcgtagt aattttagcg gagcctgcca gtttcagctt acgcttttac
     1561 tctcatttac gctgctgagg ctggtatatg ttgcattttt agtttagagt ttacttaatt
     1621 tagaaaatac ttaaataaat atgactgaac tcgcgcaatt acaggccagt accgaacagg
     1681 cagcggcctt attgaaagca atgagccacc ctaaacggtt gctgattctg tgcatgctta
     1741 gcggttcccc cggcaccagc gcgggagagc tgacgcgcat taccggactg agcgcctctg
     1801 cgacatcaca gcatctcgct cgtatgcggg acgaagggct tatcaacagc caacgggatg
     1861 cccaacgcat tctatattcc attaaaaatg aggcggtaaa tgccattatc gccaccctga
     1921 aaaatgtcta ttgtccgtaa ggagtcacta tggctttgac aaccatttcg ccgcatgatg
     1981 cacaagaatt aatcgcacgc ggcgcaaagt taatcgatat tcgtgacgct gatgaatatc
     2041 ttcgtgaaca tattcctgaa gcagatctgg ctccattatc cgtgctggaa cagtcaggtc
     2101 ttccacctaa attacgtcgc gagcaaatta tattccactg ccaggcaggt aaacgcacca
     2161 gtaataacgc cgataaatta gcagcgattg ccgcccccgc agaaatattt ttactcgaag
     2221 atgggattga tggctggaaa agagctggat tgccagtagc ggtaaataaa tctcaaccct
     2281 tgccgttaat gcgccaggtg cagatcgctg cgggtggttt aatattaatc ggcgttgtac
     2341 tgggttatac cgtaaatagc ggtttcttct tattaagtgg ctttgttggt gccgggttac
     2401 tgtttgcagg aatcagcggt ttttgtggaa tggcaaggtt gttagataag ataccgtgga
     2461 accaacgagc ttgagaagcg acgccggacg cgccctagca gcgacatccg gcctcagtaa
     2521 ttagatcagg aaatcgtcga gagatttacc ttctgccagc gcctgagcaa ttggcttcgg
     2581 tgtacggccc tgaccggtcc aggttttagt ttcaccgtta acatcagtga atttatattt
     2641 cgccggacgc ggctggcgtt ttttaccagc acgtggtgca gcagcagagc tattacccaa
     2701 taactcttcc gggttaattc cgtcagcttt catcagctcc agccaggtgc taattttttc
     2761 ctgacgctca gccagttcac gctgctgctg ttcttcttct tcacgtcttt ctttagtgac
     2821 aaccctgaat ttttcgagca tttcttcaag aacgtcaatg gagaattcgc gagccatcgc
     2881 acggagggtg cgaatgttat ttaaactttg taacattacg gacataataa agaaaacctt
     2941 ttaacgccaa aacaaaaaag tatttcgtcg cgtagtgtaa gcgatcactt attatatttc
     3001 cacaaaattt aatctattta cagtcctgcg cgagattatt tcagctgtta ttttctgaaa
     3061 ctctcagaat aatttcaaga ttatatatta gccattttga tgagtatttt aggttattgg
     3121 tgaacagagt ggcgctctct gctcgctaac tccgtaagcc tgacaaaacc gaagtgaata
     3181 aaaatgcaat ataacagatt aaaagaaaac acccctcact ggtgctttct gctgttaatg
     3241 tacattttct ggcatcaaaa atgctgtaaa tgacccggtc acgacaaaca ccgaattctc
     3301 tacgcttata gcgttacctc ccccccaaac tttcattagt gaaattaaag gcaaggccat
     3361 atccaattga tttaattgaa aataaatcat atcggcatgt aaaaatccac aatgtacaaa
     3421 aaacgagccg ttacggaata ttttatctac aaaagctgac taaataaaaa tttttcacta
     3481 atttattagt catagccagc gatatacgct atgcgaaaat acagatggca atgagatcca
     3541 ctgctttcat ctccattaac atcccattac gcttttatta aggagcatta gcatgttctc
     3601 accgcagtca cgcttgcgtc atgcagttgc agatacgttc gcgatggttg tttactgttc
     3661 tgtcgtgaac atgtgtattg aagttttcct ctccggaatg agcttcgaac agtcttttta
     3721 ttccagattg gtagcgattc cggtgaacat cttaattgca tggccatacg gtatgtaccg
     3781 cgatctgttt atgcgcgcgg cacgcaaagg ctggataaaa aatctggctg atatcctggc
     3841 ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg ttagtggtgg gcgcagactg
     3901 gcatcagatt atggcggcgg tcagttcaaa catcgttgtt tcgatgttga tgggggcggt
     3961 ttatggctac ttcctcgatt attgccgccg actgtttaaa gtcagccgtt accagcaggt
     4021 aaaagcctga ctcttctttt cgcgactggc atcgccagtc gcgtctttaa ctggcttcgc
     4081 caaataaacc attcaaataa cgttcaagcg caacacgcga actaaagcca tgcgggatac
     4141 cgtaatgctc gtgtcgctgt cgctcgcctg ctaaatagag cggaaattgc tggtaatgat
     4201 cgcaagtttt aatatcggaa gcgggttcag ccagtgttga actgcgttct tttaatgttg
     4261 gatgaattac cagagcggta cgtcccattc gcgcttcgcg attaacatag acataatttt
     4321 ccccacggcg gtaaccatac gctttctggg ttaccacatc gacagtaaaa ccgacttttt
     4381 caagtacgcg cgccacctcg tctggtcgta aatacatatt tgatcctcgt tattttcaac
     4441 cgcgggccta ccttacctga ttgcgcattc acaacgcttt cagaaaagtc cataaagccg
     4501 cgatgaacct cagttaagaa aattatggtc tacactgaaa attacatcga attctaatgg
     4561 gggatcatat gtttaaccga ccgaaccgca acgacgttga tgatggcgtg caggatatcc
     4621 agaatgatgt caatcaatta gctgacagcc tcgaatctgt attgaagtcc tggggcagcg
     4681 acgccaaagg ggaagctgaa gccgcacgca gtaaagctca ggcgttgctg aaagaaacca
     4741 gggcgcgaat gcatggtcgt actcgcgtcc agcaagccgc gcgcgatgcc gttggctgcg
     4801 cggattcttt tgttcgtgaa agaccctggt gtagcgtggg tacagcagct gcggtaggta
     4861 tttttatcgg cgcacagtta agcatgcgca aatcgtagtg caaaaatgat aataaatacg
     4921 cgtctttgac cccgaagcct gtcttcgggg tttctttttg cctggtgaat cacaaaaatc
     4981 cccctccccc gtcacgctca tatccagggt aatttcgatc actatttgct atatattgtg
     5041 tggttgaatc ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac
     5101 gcgtatcatc gcgccgtatc ttcattttaa acagaaatac gaatcatgcg cataactatt
     5161 tatactcgta acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc
     5221 tttgattttg aaatgattaa tgtcgatcgc gttcctgaag cagcggaagc gttgcgtgct
     5281 cagggctttc gtcagttgcc ggtagtgatt gctggcgatc ttagctggtc tggtttccgt
     5341 ccggacatga ttaaccgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg
     5401 tctacttctc cagcagctcc gaaaacacgc agcgttttat cgaacgttta ggtctgcccg
     5461 cggtgcgcat cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga
     5521 tcgtgccctc ttacggcggc ggcggtacgg ctggcgcggt gccacgacag gtaattcgct
     5581 ttttaaacga cgagcacaac cgggcgttgc ttcgcggcgt tattgcttct ggtaatcgca
     5641 actttggtga ggcgtatggc cgcgccggag atgtgattgc ccggaaatgc ggcgtgccgt
     5701 ggctgtaccg ttttgaactc atgggtacgc aaagcgatat cgaaaacgtt cgtaaaggag
     5761 taaccgaatt ttggcaacga caaccgcaga atgcctgacg caggaaacga tggattacca
     5821 cgcgctgaat gcgatgctta atctttacga tagcgcaggt cgcattcagt tcgataaaga
     5881 ccgccaggcc gttgacgcct ttattgcgac gcatgtgcgt ccgaacagtg tgaccttcag
     5941 tagccagcag cagcgcctga actggctggt caacgaaggt tactatgatg aaagcgttct
     6001 taatcgctac tctcgcgact ttgtcattac gctgtttgcc cacgcacaca ccagcggttt
     6061 tcgtttccag acattcctcg gggcatggaa gttttacacc agctatacgt tgaagacatt
     6121 cgacggtaaa cgttatctgg aagattttgc cgatcgagtc acgatggtgg cgctgacgct
     6181 ggcacaaggc gatgagacgc tggcgttgca actgaccgat gaaatgctgt caggacgctt
     6241 tcagccagcc acgccaacat tcctcaactg cggtaagcag cagcgcggcg aactggtttc
     6301 ctgttttttg ctgcgtattg aagacaatat ggagtcgatt ggtcgggcgg taaattccgc
     6361 actgcagctg tcgaaacgcg gcggcggcgt agcatttttg ctgtcgaatc tgcgggaagc
     6421 gggcgcgcca attaaacgta ttgaaaatca atcttctggc gtaattccgg tgatgaaaat
     6481 gttggaagac gcattttcct atgccaacca actcggcgcg cgtcaggggg caggtgcagt
     6541 ctatttacat gctcatcatc ccgatattct gcgttttctc gacacgaaac gggaaaatgc
     6601 cgacgaaaaa atccgcatta aaacactgtc gctgggcgtg gtgatcccgg atatcacttt
     6661 ccatctggca aaagagaatg cgcagatggc gctgttttcg ccctatgacg ttgagcgagt
     6721 ttatggcaag ccgtttgccg atatcgccat tagcgaacac tatgacgaac tggttgccga
     6781 tgaacgcatc cgcaaaaaat acctcaacgc ccgtgatttc ttccagcgac tggcagaaat
     6841 ccagtttgag tccggctatc cctacatcat gtatgaagac acagtaaacc gtgctaaccc
     6901 tatcgccggg cgcataaata tgagtaatct ctgctcagaa attttgcagg ttaacagcgc
     6961 ctcagagtat gacgagaatc tcgactatgc ccgcacaggc catgatattt cctgcaattt
     7021 aggttcgttg aatattgcgc acaccatgga ttcccccgat ttttcccgca cggtagagac
     7081 tgccgtgcgc ggtttaacgg cggtgtcaga tatgagtcat atccgcagcg tgccgtccat
     7141 cgaagccgga aatgccgcct cgcacgccat cggactgggg cagatgaatt tacatggtta
     7201 tctggcgagg gaaggcatcg cttatggttc gccggaagcc ctggatttca ccaatctcta
     7261 tttctatacc atcacctggc acgcactgcg tacctcgatg ttgctggcgc gcgaacgcgg
     7321 tgaaaccttc gccgggttca aacagtcacg ctatgccagt ggtgaatatt tcagccaata
     7381 tctgcaaggt aactggcagc cgaaaacggc gaaagttggc gaactgtttg cccgcagcgg
     7441 tattacgtta cctacccgtg agatgtgggc gcaactgcgc gacgacgtga tgcgctacgg
     7501 catatacaac cagaatctcc aggcggtgcc gccaaccggg tctatctctt atatcaacca
     7561 tgctacgtcg agtattcatc cgattgtggc gaaagtagag atacgcaaag agggcaaaac
     7621 aggacgcgtt tactaccctg ccccgtttat gactaacgaa aatctggcgc tgtatcagga
     7681 cgcttacgaa attggcgcag aaaagatcat cgacacctac gcggaagcga ctcgccatgt
     7741 cgatcagggg ctgtcgctga cgctgttttt ccccgatacc gccaccactc gcgatatcaa
     7801 caaagcgcag atttacgcct ggcgcaaagg tatcaaaacg ctctattaca tccgcctgcg
     7861 tcagatggcg ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac tttaaggaat
     7921 atctatgaaa ctctcacgta tcagcgccat caactggaac aagatatctg acgataaaga
     7981 tctggaggtg tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg tgccgctgtc
     8041 gaacgatatt cccgcctggc agacattaac tgtcgtagaa caacaactga cgatgcgcgt
     8101 ttttactggc ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc cttcgctgat
     8161 gcccgatgcg ctcacgcctc atgaagaggc ggtattatcg aatatcagct ttatggaagc
     8221 ggttcatgcc cgctcttaca gttcgatttt ctcgacgctg tgccagacca aagatgtcga
     8281 tgccgcctac gcctggagtg aagaaaacgc accgttgcag cgaaaagctc agattattca
     8341 gcaacattat cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt ttcttgaatc
     8401 ttttttgttc tattccggtt tctggctgcc gatgtatttt tccagccgcg gaaagctgac
     8461 caataccgcg gacctgatcc gtctgattat ccgcgatgaa gcagtccacg gttactacat
     8521 aggctataaa tatcagaaaa acatggaaaa gatatctctg ggacaacgtg aagagttgaa
     8581 gagtttcgcc ttcgatttgt tgctggaact ctacgacaac gagttgcaat acaccgatga
     8641 gctgtacgcc gaaaccccgt gggctgacga tgtgaaagcg tttctctgtt acaacaccaa
     8701 taaggctttg atgaatctgg gctacgaacc gttatttccc gcagaaatgg cggaagtgaa
     8761 tccggcaatc ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg
     8821 ttcaggctcc tcttatgtga tggggaaagc ggttgaaaca gaagatgaag actggaattt
     8881 ctgagggtgt tattttcaaa aatatcacta cccgcagcag ggaaataatt cccgccaaat
     8941 agctttttat cacgcaaata ttctgtagtg atctacactg atactctgtt gcattattcg
     9001 cctgaaacta caatattcag gcgttttttc gctatctatg acaaaaaata tcaacttttc
     9061 tcgatttgct ctcagccctt atatcacgga aaattccggc gatttgctcg catcaatatt
     9121 catgccacat ttgccctcag gggttgcctc agattctcag tatgttaggg tagaaaaaag
     9181 tgactattcc cattgggtaa tatatcgaca taaacaaata aaggaatcat tctattgcat
     9241 ggccattaaa ttagaaatta aaaatcttta taaaatattt ggtgagcatc cacagcgagc
     9301 gttcaaatat atcgaacaag gactttcaaa agaacaaatt ctggaaaaaa ctgggctatc
     9361 gcttggcgta aaagacgcca gtctggccat tgaagaaggc gagatatttg tcatcatggg
     9421 attatccggc tcgggtaaat ccacaatggt acgccttctc aatcgcctga ttgaacccac
     9481 ccgcgggcaa gtgctgattg atggtgtgga tattgccaaa atatccgacg ccgaactccg
     9541 tgaggtgcgc agaaaaaaga ttgcgatggt cttccagtcc tttgccttaa tgccgcatat
     9601 gaccgtgctg gacaatactg cgttcggtat ggaattggcc ggaattaatg ccgaagaacg
     9661 ccgggaaaaa gcccttgatg cactgcgtca ggtcgggctg gaaaattatg cccacagcta
     9721 cccggatgaa ctctctggcg ggatgcgtca acgtgtggga ttagcccgcg cgttagcgat
     9781 taatccggat atattattaa tggacgaagc cttctcggcg ctcgatccat taattcgcac
     9841 cgagatgcag gatgagctgg taaaattaca ggcgaaacat cagcgcacca ttgtctttat
     9901 ttcccacgat cttgatgaag ccatgcgtat tggcgaccga attgccatta tgcaaaatgg
     9961 tgaagtggta caggtcggca caccggatga aattctcaat aatccggcga atgattatgt
    10021 ccgtaccttc ttccgtggcg ttgatattag tcaggtgttc agtgcgaaag atattgcccg
    10081 ccggacaccg aatggcttaa ttcgtaaaac tcctggcttc ggcccacgtt cggcactgaa
    10141 attattgcag gatgaagatc gcgaatatgg ctacgttatc gaacgcggta ataagtttgt
    10201 cggcgcagtc tccatcgatt cgcttaaaac cgcgttaacg cagcagcaag gtcttgatgc
    10261 ggcgctgatt gatgcgccgt tagcagtcga tgcacaaacg cctcttagcg agttgctctc
    10321 tcatgtcgga caggcaccct gtgcggtgcc cgtggtcgac gaggaccaac agtatgtcgg
    10381 catcatttcg aaaggaatgc tgctgcgcgc tttagatcgt gagggggtaa ataatggctg
    10441 atcaaaataa tccgtgggat accacgccag cggcggacag tgccgcgcaa tccgcagacg
    10501 cctgggggac accgacgact gcaccggctg acggcggtgg tgctgactgg ctgaccagta
    10561 cgcctgcgcc aaacgtcgag cattttaata ttctcgatcc gttccataaa acgctgatcc
    10621 cgctcgacag ttgggtcact gaagggatcg actgggttgt tacccatttc cgtcccgtct
    10681 tccagggcgt gcgcgttccg gttgattata tcctcaacgg tttccagcaa ttgctgctgg
    10741 gtatgccagc gccagtggcg attatcgttt tcgctctcat cgcctggcag atttccgggg
    10801 tcggaatggg cgtggcgacg ctggtctcgc tgattgccat cggcgcaatc ggtgcctgtt
    10861 cgcaggcaat ggtgactctg gcgctggtgt taactgccct gctgttctgt atcgtcatcg
    10921 gtttgccgtt ggggatatgg ctggcgagaa gtccgcgagc ggcgaaaatt attcgtccac
    10981 tgcttgatgc catgcagacc acgccagcgt ttgtttatct ggtgccaatc gtcatgctat
    11041 ttggtatcgg taacgtgccg ggcgtggtgg tgacgatcat ctttgcgctg ccgccgatta
    11101 tccgtctgac cattctgggg attaaccagg ttccggcgga tctgattgaa gcctcgcgct
    11161 cattcggtgc cagcccgcgc cagatgctgt tcaaagttca gttaccgctg gcgatgccga
    11221 ccattatggc gggcgttaac cagacgctga tgctggccct ttctatggtg gtcattgcct
    11281 cgatgattgc cgtcggcggg ctgggtcaga tggtacttcg cggtatcggt cgtctggata
    11341 tggggcttgc caccgttggc ggcgtcggga ttgtgatcct cgccattatc ctcgatcgcc
    11401 tgacgcaggc cgttgggcgc gactcacgca gtcgcggcaa ccgtcgctgg tacaccactg
    11461 gccctgttgg tctgctgacc cgcccattca ttaagtaact ctgcacttgc ccggtgacgc
    11521 cgggcattat caccctgcca aaaaaaggaa taacaatgcg acatagcgta ctttttgcga
    11581 cagcgtttgc cacgcttatc tctacacaaa cttttgctgc cgatctgccg ggcaaaggca
    11641 ttactgttaa tccagttcag agcaccatca ctgaagaaac cttccagacg ctgctggtca
    11701 gtcgtgcgct ggagaaatta ggttataccg tcaacaaacc cagcgaagtg gattacaacg
    11761 ttggctacac ctcgcttgct tccggcgatg caaccttcac cgccgtgaac tggacgccac
    11821 tccatgacaa catgtacgaa gctgccggtg gcgataagaa attttatcgt gaaggggtat
    11881 ttgttaacgg cgcagcacag ggttacctga tcgataagaa aaccgccgac caatacaaaa
    11941 tcaccaacat cgcacaactg aaagatccga agatcgccaa actgttcgat accaacggcg
    12001 acggtaaagc ggatttaacc ggttgtaacc ctggctgggg ctgcgaaggt gcgatcaacc
    12061 accagcttgc cgcgtatgaa ctgaccaaca ccgtgacgca taatcagggg aactacgcag
    12121 cgatgatggc cgacaccatc agtcgctaca aagagggcaa accggtgttt tattacacct
    12181 ggacgccgta ctgggtgagt aatgagctga agccagggaa agatgtggtc tggttgcagg
    12241 tgccgttctc cgcactgccg ggcgataaaa atgccgatac caaactgccg aatggcgcga
    12301 attacggctt cccggtcagc accatgcata tcgttgccaa caaagcctgg accgagaaaa
    12361 atccggcggc agccaaactg tttgccatta tgcagttgcc agtggccgat attaacgccc
    12421 agaacgccat tatgcatgac ggcaaagcct cagaaggcga tattcagggc cacgttgatg
    12481 gctggatcaa agcccaccag cagcagttcg atggctgggt gaatgaggcg ctggcagcgc
    12541 agaagtaatt tttattcggg cggataaggc gttttcgcct catccgccgc tctgtacaca
    12601 atgcctgatg cgacgctgac gcgtcctatc atgcctacaa accacatcgc acatttcaac
    12661 aagctatctt tcatcccata ttcatcaaca tccgctatta ttgatttcca gcttaatcat
    12721 cacctgatga acaaaaataa tgactaaacc taatcatgag cttagcccgg cgctgatcgt
    12781 gctgatgtct atcgccaccg gtctggcggt agccagtaac tattacgccc agccattgct
    12841 cgacaccatc gcgcgtaact tttccctttc cgccagttcg gcaggcttta ttgttaccgc
    12901 cgcgcagttg ggctatgccg caggtctact gtttcttgtt cccctcggtg atatgtttga
    12961 acgccgccgc ctgattgtct cgatgacctt actggcagcg ggcggtatgt taatcactgc
    13021 cagcagtcag tcactggcga tgatgatcct cggtacggca ttaaccggtt tattctcagt
    13081 cgtggcacaa attctggttc cgctggcagc gacgctggct tcaccggata aacgcggcaa
    13141 agtggttggc accattatga gcggtctgct gttggggata ttgctggcac gaaccgttgc
    13201 cgggttactg gcaaacctcg gcggctggcg caccgtcttt tgggttgctt cggtgttaat
    13261 ggcactgatg gcgctggcat tatggcgtgg tctgccacaa atgaaatcag aaacccacct
    13321 caactaccca cagttgttgg gttccgtttt cagcatgttc atcagcgata agatcctgcg
    13381 cacccgcgcg ttgctgggct gcatgacctt tgccaacttc agcattctct ggacctcaat
    13441 ggcctttttg cttgccgctc caccttttaa ctacagcgat ggtgacattg gtctgtttgg
    13501 gcttgcggga gctgccggag cgttgggcgc tcgtccggcg ggcggttttg ccgacaaggg
    13561 caaatcgcac cacaactttc ggtctgcttc tgctgttact ttcatggctg gcaatctggt
    13621 ttggtcacac ttccgtactg gcgttgatta tcggcattct ggtgctggac ctcaccgtac
    13681 agggcgtgca tatcactaac cagacggtaa tttatcgaat acatcctgat gcgcgtaatc
    13741 gcctgaccgc aggttacatg accagctact ttattggcgg tgccgccggt tcgctaattt
    13801 cagcctcagc ctggcaacat ggcggttggg ctggcgtttg tctggctggc gcgacgattg
    13861 ccctggttaa cttactggtc tggtggcgag gttttcatcg tcaggaagcc gcaaattaag
    13921 caatcagcaa ccgttttcgt ggctttacac ttataagggt gttaagaagc ccatcagtct
    13981 gataaggtta agatattcat tcagtctatt tataatatta acaatcgtta agcgtacact
    14041 ctatggaaag ccctactcca cagcctgctc ctggttcggc gaccttcatg gaaggatgca
    14101 aagacagttt accgattgtt attagttata ttccggtggc ctttgcgttc ggtctgaatg
    14161 cgacccgtct gggattctct cctctcgaaa gcgttttttt ctcctgcatc atttatgcag
    14221 gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg gagtagtttg tgggttgctg
    14281 cactgaccgt catggcaatg gatgttcgcc atgttttgta tggcccgtca ctgcgtagcc
    14341 gtattattca gcgtctgcaa aaatcgaaaa ctgccctatg ggcgtttggc ctgacggatg
    14401 aggtttttgc cgccgccacc gcaaaactgg tacgcaataa tcgccgctgg agcgagaact
    14461 ggatgatcgg cattgccttc agttcatggt catcgtgggt atttggtacg gtaatagggg
    14521 cattctccgg cagcggcttg ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta
    14581 tgcttccggc actctttatg agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt
    14641 gcgttaccgc agctttagtt ggtgcccttg caggcgtaac gctattttct attcccgtcg
    14701 ccattctggc aggcattgtc tgtggctgcc tcactgcgtt aatccaggca ttctggcaag
    14761 gagcgcccga tgagctatga ggttctgctg cttgggttac tggttggcgc ggcgaattat
    14821 tgcttccgct atttgccgct gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc
    14881 gcggtaggta ttttgctcga caccattggc atcgcctcga tatgcgctct gctggttgtc
    14941 tctaccgcac cagaagtgat gcacgataca cgccgtttcg tgcccacgct ggtcggcttc
    15001 gcggtactgg gtgccagttt ctataaaaca cgcagcatta tcatcccaac actgcttagt
    15061 gcgctggcct atgggctcgc ctggaaagtg atggcgatta tataacccac aagaatcatt
    15121 tttctaaaac aatacattta ctttatttgt cactgtcgtt actatatcgg ctgaaattaa
    15181 tgaggtcata cccaaatgga tagttcgttt acgcccattg aacaaatgct aaaatttcgc
    15241 gccagccgcc acgaagattt tccttatcag gagatccttc tgactcgtct ttgcatgcac
    15301 atgcaaagca agctgctgga gaaccgcaat aaaatgctga aggctcaggg gattaacgag
    15361 acgttgttta tggcgttgat tacgctggag tctcaggaaa accacagtat tcagccttct
    15421 gaattaagtt gtgctcttgg atcatcccgt accaacgcga cgcgtattgc cgatgaactg
    15481 gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg atcgccgctg cctgcatctg
    15541 caattaacgg aaaaaggtca cgagtttttg cgcgaggttt taccaccgca gcataactgc
    15601 ctgcatcaac tctggtccgc gctcagcaca acagaaaaag atcagctcga gcaaatcacc
    15661 cgcaaattgc tctcccgtct cgaccagatg gaacaagacg gtgtggttct cgaagcgatg
    15721 agctaacgcg tcatctcgct caaaaatcca gatttataaa agaaaaaatg accggccagc
    15781 atcgcaacat gctggccttt ttggcaagca ggtcggctca gccgatgagt taagaagatc
    15841 gtggagaaca atatgagcgc aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag
    15901 agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc tctttataat tattgccgta
    15961 gcgataggga tttattggtt tttggtactg cgtcatttcg aagaaaccga tgacgcatac
    16021 gtggcaggga atcagattca aattatgtct caggtgtctg gcagcgtgac gaaagtctgg
    16081 gccgataaca ccgattttgt aaaagaaggc gatgttctgg tcactctcga cccgacagat
    16141 gctcgccagg cgtttgaaaa agccaaaact gcactggctt ccagcgttcg ccaaacccac
    16201 cagctgatga ttaacagcaa acagttgcag gcgaatattg aggtgcaaaa aatcgccctc
    16261 gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg gcaatgccaa cctgattggt
    16321 cgcgaagagc tgcaacacgc ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc
    16381 gctattcaac aatacaatgc caatcaggcg atgattctgg ggactaaact ggaagatcag
    16441 cctgccgtgc aacaggctgc caccgaagta cgtaacgcct agctggcgct ggagcgtacc
    16501 cgtattgtca gtccgatgac cggttatgtt tcccgtcgtg ccgtgcaacc gggtgcgcaa
    16561 attagcccaa cgacgccgct gatggcggtc gttccagcca ccaatatgtg ggtggatgcc
    16621 aactttaaag agacgcagat tgccaatatg cgcatcggtc agccggtcac catcaccacc
    16681 gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca
    16741 ggtagcgcgt tctcactgct tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt
    16801 cagcgtctac ctgtgcgtat cgaactggac cagaaacagc tggagcaata cccgctgcgc
    16861 attggtttgt ccacgctggt gagcgtcaat accactaacc gtgacggtca ggtactggca
    16921 aataaagtac gttcaacacc ggtcgcggta agtaccgcgc gtgaaatcag cctagcacct
    16981 gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt
    17041 gatgcaacag caaaaaccgc tggaaggcgc gcaactggtc attatgacga ttgcgctgtc
    17101 actggcgaca ttcatgcagg tgctggactc caccattgct aacgtggcga tccccactat
    17161 cgccgggaat ctgggctcat cgctcagcca aggaacgtgg gtaatcactt ctttcggggt
    17221 ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa
    17281 actgttcctt tggtccacca tcgcctttgc tattgcgtcg tgggcgtgtg gtgtctccag
    17341 cagcctgaat atgctgatct tcttccgcgt gattcagggg attgtcgccg ggccgttgat
    17401 cccgctttcg caaagtctat tgctgaataa ctacccgcca gccaaacgct cgatcgcgct
    17461 ggcgttgtgg tcgatgacgg tgattgtcgc gccaatttgc ggcccgatcc tcggcggtta
    17521 tatcagcgat aattaccact ggggctggat attcttcatc aacgtgccga ttggcgtggc
    17581 ggtggtgttg atgacactgc aaactctgcg cggacgtgaa acccgcaccg aacggcggcg
    17641 gattgatgcc gtggggctgg cactgctggt tattggtatc ggcagcctgc agattatgct
    17701 cgaccgcggt aaagagctgg actgtttttc atcacaggaa attatcattc ttaccgtggt
    17761 ggcggtggtg gctatctgct tcctgattgt ctgggagctg accgacgata acccgatagt
    17821 cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc
    17881 gtatatgctc tacttcggcg ctattgttct gctgccgcag ttgttgcagg aggtctacgg
    17941 ttacacggcg acctgggcag gtttggcctc tgcgccggta gggattattc cggtgattct
    18001 gtcgccgatt atcggccgct tcgcgcataa actggatatg cggcggctgg taaccttcag
    18061 ctttattatg tatgccgtct gcttctactg gcgtgcctat acctttgaac caggtatgga
    18121 ttttggcgcg tcggcctggc cgcagtttat ccaggggttt gcggtggcct gcttctttat
    18181 gccgctgacc accattacgc tgtctggttt gccaccggaa cgactggcgg cggcatcgag
    18241 cctctctaac tttacgcgaa cgctggcggg gtctatcggc acgtcgataa ccacgaccat
    18301 gtggaccaac cgcgagtcga tgcaccatgc gcagttgact gagtcggtaa acccgttcaa
    18361 cccgaatgcc caggcgatgt acagtcaact ggaagggctt gggatgacgc aacagcaggc
    18421 atcaggctgg attgcccagc agatcaccaa tcaggggctg attatttccg ccaatgagat
    18481 cttctggatg tcagccggga tattcctcgt actgctgggg ctggtgtggt ttgctaaacc
    18541 gccatttggc gcaggtggcg gcggaggcgg tgcgcactaa gtacaactaa gccagttcat
    18601 ttgaactggc ttttttcaat taattgtgaa gatagtttac tgactagatg tgcagttcct
    18661 gcaacttctc tttcggcagt gccagttctt cgttgctgtt gatgcgtacg tcacgttcca
    18721 gaatgctacg cgcaatatcc tgcgcttcct gcaacgagtg catctggtaa gtgccacact
    18781 ggtagacgtt cagttccggg atctgattct gatcctgcac tttcagcacg tcttccattg
    18841 ccgctttcca ggcatcagca acacgctgct catctggcgt accaatcaga ctcatataaa
    18901 aaccggtgcg gcagcccatt ggcgagatat cgataatctc tacaccatta ccgttaagat
    18961 ggttacgcat aaaaccagca aacaggtgct ccagggtatg gatccctctt tctggcatca
    19021 cttctttgtt cggcacgcag aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg
    19081 tgttcattgt tttcgccacc cgaactgcag gcgcttccat ccgggtatga tcgactgtga
    19141 agctatctaa caacggcatt tagccacctc cggtaatttt tttaaaaatt ttctgaactc
    19201 tttcttccca ggcgagtctg agtatatgaa agacgcgcat ttgttatcat catccctgaa
    19261 ttcagagatg aaattttggc cactcacgag tggccttttt cttttctgtc aggcgtgttt
    19321 ttccagccac accgcaaacg gttcggtatc agcggtttcc atttcctgct gacggcgttc
    19381 agatgcctcg cgctcggcta caaaatcctc ttcgcgcaga atttccagcg gctcttcacg
    19441 cagcagatta cggtaggctt ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat
    19501 catagacctt aagatacggg cagagaaagt cagatcggga ttatcgaagc aggcaaccag
    19561 ttcatcacac actttctgat acgcttcgcc gccgttaatg ctatccagcg tttgcgcgac
    19621 gcgtttcaga tcgtggaaca gatctttacc cacctgcagt aacgggaact gcgcggtttc
    19681 gcagccgata cccagcgtca ggcccggttt gcgaccttca aggatcaccc ggttccagtt
    19741 aacgcgcgta caggcaagtt cgctactgct catttccggt gcatcagcca gcgcacacca
    19801 gaccataaac aggtcgagga atcgcacctg ctgttcatct acaccaatcg gcgagaacgg
    19861 gttgatgtcc agcgaacgca cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga
    19921 aggtgactcg ccgctgcggg taacgcgttt tggacgaatc ggcgcataca gttcgttttc
    19981 aatctgcagc acgttgctat tgatttgcag cctcttaccg tctttctcaa taccaatctt
    20041 cgcgtactct tccgatggcg ttttgattgc ctgtttaagg cccgctacgt actcgtaaag
    20101 atcgttgaag gtaataccaa gattgctttg cgatttattg gtatagccga gatcgctcaa
    20161 acgaagagag gtcgcatacg gcaggtaata cataccgcac tcggttttct caaacggcag
    20221 cgacgttggt tttccttgca ggaaagaaga acaaatcgcc ggagatgcac caaacagata
    20281 aggaatgacc caaccgaaac gatagtaatt gcggataacg cggaaatagc ccgcagaaat
    20341 tttctctttg gcatcagcgc ccgagatatc accgcacttc gcttgccaga atgccattgg
    20401 caaagagaaa ttgtagtgca cgccggaaat ggtttgcatc agcgcgccgt agcgattttt
    20461 cagcccttca cgatacagcg ttttaaagcg tccggtgtta gaagtgccgt actgtgccag
    20521 ttcgatgtcc tgaccttctg cgatgtagca tggcatactt aacggccaca tccgctcatc
    20581 gcccatattg cgcgccgtat aacgatgcag atcgcgcata aaggtcagca tatgttcaat
    20641 atcaccatcc actggtgtaa tgaattccag caatgcttcc gcaaaatcgg tagtaatcca
    20701 tttgtgcgtc agtgcggaac ctaatgcttc aggatgacct gttgttgcca gtgtgccatc
    20761 agcattaaca cgcaaagttt cgcgctccag cccacgctgt atccccttta acgcctgagg
    20821 atgtttttcc agccaggcca gcgcctgtga tacgtccggg atcaaattga cctcccgcct
    20881 gtcaaaatcg ttttaattag cataactgta atggtgacca tatgtgcagg cctacaatta
    20941 gtgccaccac atcatgccct gaacggtcgc tgctgcaact gcaacatagc gtaacgcttt
    21001 accaaggcac aaaaaaaaga ttactggccc ccaggagatg cgcatccatc ccgctaacag
    21061 acacagtaaa tcgccaacca cgggcatcca gcttaataat agcgtgactg caccatagcg
    21121 tttcagccag ccggtagctt tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg
    21181 cccaaggata acgttagtta accctccaag gctattaccc attgttgctg ttaaaactaa
    21241 aacctaggga tgactgatcc cggaaagcaa cattgccacc agcacgactt cggagttgcc
    21301 gggtaatagt gtagcgctga gaaaactact ggcaaacaac gagaaaagcg ataacgcttc
    21361 actcacagca agcgaacatc cacagcgtcc atgcctgctg cacgggccgc ctgaataccg
    21421 aaatcggcat cttcaaagac cacacactgc gtcggttgca cgcccatacg ctgcgcgcac
    21481 aacaaaaatg tgtctggcgc gggtttatgg tgtttgacgt gatcagcagc gacgacggcg
    21541 tcaaaataac ggcgtaatcc caggtgcgcc agcaatgcct ctgcgatggc gctttcactc
    21601 cccgttccta cagccattgg gcgacgacca tgccaacttt tcaccacttc aacaagagga
    21661 agcggctcga cactatccag cagcatactt cttactgctt ctgttttttc acgcgctaac
    21721 gcatgcgggt cgagatcggc ctgattcagc tcaataattg cctgagcaat acgccaggtg
    21781 ggcgatccat taagcgcaat catcgcctga atatcgtact gaagaccgta gtgccctaat
    21841 acttcgcgcc acgctttacg gtgcgtaggc tccgtatcca ggattgtgcc atccatatca
    21901 aaaattaaac ctgcataacg ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct
    21961 actttagcgt aattgcttga ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat
    22021 ggataaagta gttatttaga aatgagatat ttttgaagga aattttttgg agaa
//
LOCUS       DAJTRQ010000057        21800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000057 DAJTRQ010000000
VERSION     DAJTRQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..2005
                     /gene="speF"
                     /locus_tag="OO332_002265"
     CDS             <1..2005
                     /gene="speF"
                     /locus_tag="OO332_002265"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR5942726.1"
                     /translation="NEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRP
                     PFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMG
                     DLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHK
                     SNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPF
                     RLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLN
                     ENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFY
                     PLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQS
                     YETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGV
                     PATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLA
                     EVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEA
                     NYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGIN
                     LLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="OO332_002266"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="OO332_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5942727.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="OO332_002267"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="OO332_002267"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5942728.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="OO332_002268"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="OO332_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5942729.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="OO332_002269"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="OO332_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5942730.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6677..6970
                     /gene="ybfE"
                     /locus_tag="OO332_002270"
     CDS             6677..6970
                     /gene="ybfE"
                     /locus_tag="OO332_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5942731.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="OO332_002271"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="OO332_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5942732.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="OO332_002272"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="OO332_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5942733.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="OO332_002273"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="OO332_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5942734.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="OO332_002274"
     CDS             complement(8459..8785)
                     /locus_tag="OO332_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942735.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="OO332_002275"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="OO332_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5942736.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVNTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="OO332_002276"
     CDS             complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="OO332_002276"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5942737.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="OO332_002277"
     CDS             complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="OO332_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5942738.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14953..15753
                     /gene="nagB"
                     /locus_tag="OO332_002278"
     CDS             14953..15753
                     /gene="nagB"
                     /locus_tag="OO332_002278"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5942739.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15813..16961
                     /gene="nagA"
                     /locus_tag="OO332_002279"
     CDS             15813..16961
                     /gene="nagA"
                     /locus_tag="OO332_002279"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5942740.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16970..18190
                     /gene="nagC"
                     /locus_tag="OO332_002280"
                     /gene_synonym="nagR"
     CDS             16970..18190
                     /gene="nagC"
                     /locus_tag="OO332_002280"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5942741.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18238..18980
                     /gene="nagD"
                     /locus_tag="OO332_002281"
                     /pseudo
     CDS             18238..18980
                     /gene="nagD"
                     /locus_tag="OO332_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19377..21041
                     /gene="asnB"
                     /locus_tag="OO332_002282"
     CDS             19377..21041
                     /gene="asnB"
                     /locus_tag="OO332_002282"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5942742.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21421..21497
                     /locus_tag="OO332_002283"
     tRNA            21421..21497
                     /locus_tag="OO332_002283"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21455..21457,aa:Met,seq:cat)
     gene            21506..21590
                     /locus_tag="OO332_002284"
     tRNA            21506..21590
                     /locus_tag="OO332_002284"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21540..21542,aa:Leu,seq:tag)
     gene            21614..21688
                     /locus_tag="OO332_002285"
     tRNA            21614..21688
                     /locus_tag="OO332_002285"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21646..21648,aa:Gln,seq:ttg)
     gene            21723..21797
                     /locus_tag="OO332_002286"
     tRNA            21723..21797
                     /locus_tag="OO332_002286"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21755..21757,aa:Gln,seq:ttg)
BASE COUNT         5343 a         5532 c         5508 g         5417 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatca ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgttgacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgaaaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    10321 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    10381 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    10441 gcggttgaaa ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta
    10501 cattttattt aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg
    10561 aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg
    10621 ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca
    10681 atatattgca gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg
    10741 aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac
    10801 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
    10861 cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg
    10921 gaatgcttta cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac
    10981 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct
    11041 gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
    11101 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata
    11161 agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat
    11221 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
    11281 agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg
    11341 gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg
    11401 atagttttcg ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc
    11461 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
    11521 ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
    11581 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
    11641 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
    11701 gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg
    11761 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat
    11821 gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
    11881 agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc
    11941 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
    12001 cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt
    12061 cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc
    12121 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
    12181 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata
    12241 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
    12301 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
    12361 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
    12421 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
    12481 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
    12541 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
    12601 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca
    12661 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
    12721 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
    12781 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
    12841 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
    12901 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
    12961 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
    13021 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
    13081 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
    13141 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
    13201 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
    13261 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
    13321 gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg
    13381 taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg
    13441 ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt
    13501 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
    13561 ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc
    13621 tggcggccgg caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc
    13681 cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca
    13741 ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg
    13801 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
    13861 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga
    13921 agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc
    13981 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
    14041 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
    14101 aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg
    14161 gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg
    14221 gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac
    14281 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
    14341 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
    14401 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
    14461 atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
    14521 ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta
    14581 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
    14641 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc
    14701 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
    14761 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
    14821 ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc
    14881 tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta
    14941 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
    15001 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta
    15061 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
    15121 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
    15181 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
    15241 gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc
    15301 gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
    15361 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
    15421 actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
    15481 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc
    15541 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt
    15601 gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc
    15661 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
    15721 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct
    15781 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
    15841 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
    15901 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
    15961 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
    16021 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
    16081 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
    16141 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
    16201 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
    16261 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
    16321 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
    16381 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
    16441 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
    16501 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc
    16561 tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc
    16621 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
    16681 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
    16741 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
    16801 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg
    16861 gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
    16921 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
    16981 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
    17041 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
    17101 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
    17161 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
    17221 caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga
    17281 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac
    17341 gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct
    17401 gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
    17461 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga
    17521 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
    17581 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
    17641 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga
    17701 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
    17761 cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca
    17821 gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc
    17881 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa
    17941 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat
    18001 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct
    18061 gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
    18121 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
    18181 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
    18241 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
    18301 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
    18361 accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc
    18421 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc
    18481 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
    18541 gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc
    18601 tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc
    18661 gccaccaacc cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
    18721 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
    18781 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
    18841 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
    18901 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
    18961 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
    19021 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    19081 aattttcatg cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc
    19141 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
    19201 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
    19261 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
    19321 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    19381 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    19441 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    19501 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    19561 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    19621 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    19681 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    19741 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    19801 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
    19861 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    19921 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    19981 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    20041 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
    20101 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    20161 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    20221 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    20281 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    20341 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    20401 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    20461 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    20521 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    20581 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    20641 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    20701 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    20761 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    20821 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    20881 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    20941 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    21001 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    21061 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    21121 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    21181 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    21241 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    21301 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    21361 gcaaggctct atacgcataa tgcgccccgc aacgcagata aggtatcgcg aaaaaaagat
    21421 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    21481 tcccgtcgta gccaccatct tttttgcggg agtggcgaaa ttggtagacg caccagattt
    21541 aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa
    21601 agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    21661 cctggttcga atccaggtac cccagccatc ttcttcgagt aagcggttca ccgcccggtt
    21721 attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa
    21781 tccaggtacc ccagccatcg
//
LOCUS       DAJTRQ010000058        21664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000058 DAJTRQ010000000
VERSION     DAJTRQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..288)
                     /locus_tag="OO332_002287"
     CDS             complement(<1..288)
                     /locus_tag="OO332_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HCR5942743.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEP"
     gene            427..567
                     /locus_tag="OO332_002288"
     CDS             427..567
                     /locus_tag="OO332_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942744.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            581..1453
                     /locus_tag="OO332_002289"
     CDS             581..1453
                     /locus_tag="OO332_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5942745.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1513..2811)
                     /gene="eno"
                     /locus_tag="OO332_002290"
     CDS             complement(1513..2811)
                     /gene="eno"
                     /locus_tag="OO332_002290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5942746.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2899..4536)
                     /gene="pyrG"
                     /locus_tag="OO332_002291"
     CDS             complement(2899..4536)
                     /gene="pyrG"
                     /locus_tag="OO332_002291"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5942747.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4764..5555)
                     /gene="mazG"
                     /locus_tag="OO332_002292"
     CDS             complement(4764..5555)
                     /gene="mazG"
                     /locus_tag="OO332_002292"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5942748.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5651..7885)
                     /gene="relA"
                     /locus_tag="OO332_002293"
     CDS             complement(5651..7885)
                     /gene="relA"
                     /locus_tag="OO332_002293"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCR5942749.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(7933..9234)
                     /gene="rlmD"
                     /locus_tag="OO332_002294"
     CDS             complement(7933..9234)
                     /gene="rlmD"
                     /locus_tag="OO332_002294"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5942750.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9291..12047
                     /gene="barA"
                     /locus_tag="OO332_002295"
     CDS             9291..12047
                     /gene="barA"
                     /locus_tag="OO332_002295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5942751.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12279..13619)
                     /gene="gudD"
                     /locus_tag="OO332_002296"
     CDS             complement(12279..13619)
                     /gene="gudD"
                     /locus_tag="OO332_002296"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5942752.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13640..14980)
                     /locus_tag="OO332_002297"
     CDS             complement(13640..14980)
                     /locus_tag="OO332_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5942753.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14982..16334)
                     /gene="gudP"
                     /locus_tag="OO332_002298"
     CDS             complement(14982..16334)
                     /gene="gudP"
                     /locus_tag="OO332_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5942754.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16768..17217)
                     /locus_tag="OO332_002299"
     CDS             complement(16768..17217)
                     /locus_tag="OO332_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5942755.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17235..18017)
                     /gene="truC"
                     /locus_tag="OO332_002300"
     CDS             complement(17235..18017)
                     /gene="truC"
                     /locus_tag="OO332_002300"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5942756.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18017..18346)
                     /locus_tag="OO332_002301"
     CDS             complement(18017..18346)
                     /locus_tag="OO332_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5942757.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18968..19513)
                     /gene="syd"
                     /locus_tag="OO332_002302"
     CDS             complement(18968..19513)
                     /gene="syd"
                     /locus_tag="OO332_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5942758.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19581..20429
                     /gene="queF"
                     /locus_tag="OO332_002303"
     CDS             19581..20429
                     /gene="queF"
                     /locus_tag="OO332_002303"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5942759.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20541..>21664
                     /gene="ppnN"
                     /locus_tag="OO332_002304"
                     /gene_synonym="ygdH"
     CDS             20541..>21664
                     /gene="ppnN"
                     /locus_tag="OO332_002304"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5942760.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKL"
BASE COUNT         5361 a         5751 c         5135 g         5417 t
ORIGIN      
        1 aggctcacca cccgtaatca ccacatgccg cgcggtatat ccctgacgac caatgacagc
       61 cagcaagtct tcactgctcg ccgcccccca cttatcactc tctttggtct tcgccagaat
      121 gctgaaaagg gagacttccc gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca
      181 ggcacagcca accgggcatc cctgtaaacg aataaaaatg gcggggacgc cggtaaagta
      241 accctcacct tgcagggtct ggaacatctc gttaatcggg tactgcatag cattctctgt
      301 gaagtggata attgttaatt attgcagatc ctgccgcaac aatcatgtct tattaacatt
      361 ctgttacatg cagatttaaa gaaaaatacg aaacatatcg ttaataatta aagggagtaa
      421 cgtattatgt cagaagaaaa taaagaaaat ggatttaatc atgtcaaaat attcaccaaa
      481 attatattta ttttttctgt attagttttt aatgataacg aatctaaaat taccgatgcc
      541 gccgtcaatt tatttatcca gatttaatga gaaatttata atgcgctatt tcatactaat
      601 gttcactttc gtatgttcct ttgttgcagc ccaaccaact attgtcccac aattacagca
      661 acaagttacg ggtcttacga gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa
      721 gttagaatct attttcaata atacccaagt gcaaattgct gtattaattg tttccacaac
      781 caaaaatgag acaattgaac aatatgctac aagagttttt gacaattggc gtttaggaga
      841 tgccaaacgt aatgatggga tactgatcgt tgttgcctgg tcggatcgca ctgtccgcat
      901 ccaggtaggt tatgggctgg aagaaaaggt aaccgatgct ctggctggag atatcatccg
      961 tagcaacatg atacccgcat ttaaacaaca aaaattagct aagggattag agttagctat
     1021 aatcgctttg aataaccaac tcacttcaca acaccaatat ccggctaatc cttcagaaag
     1081 tgaatcaacg tcttccactg atcattatta ctttgctatt ttttgggtat ttgcagtgat
     1141 gttcttccct ttctggtttt ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg
     1201 agtctgtatt tcagcaatcc atcttttaga tttattcctg ttctccgata aaactttttc
     1261 cattgctgta ttttccttct tttttacttt caccatattt atggtcttta cctgtttatg
     1321 tgtacttcag aaaagagcat ctggtagaag ttatcattca gacaacagcg gttccgctgg
     1381 aggctcaagt ggcggcggat ttagcggcgg gggaggttct tctggcggtg gcggcgcatc
     1441 cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt atcaaatcag
     1501 ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg gtgctttttc
     1561 gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt cagaacggct
     1621 catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag cgatggtagc
     1681 atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat ctttcgccat
     1741 cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga tcaggatgga
     1801 gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta cgaacaggtc
     1861 gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga aaccgtccca
     1921 gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg tcagttcttc
     1981 caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag ccagaacgta
     2041 tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga tgtctttgcc
     2101 cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt cagcgttgga
     2161 acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc ctttcgcttt
     2221 cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt ctttcacagt
     2281 tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt cagcgtgctc
     2341 accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg gagtgccgtt
     2401 cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag ctttggcgtt
     2461 agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt cggtgccgtc
     2521 caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc caatcagcgc
     2581 ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac ccaggaaacg
     2641 ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag cacctgacgg
     2701 agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa cagtcgggtt
     2761 accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca ttaggttttc
     2821 ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag gtacgcgttg
     2881 ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg cggctttcac
     2941 aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg gatggaactg
     3001 gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact gatcatcccc
     3061 ggaacggccc acaacgcgca gacctgcatc ttcaatctgt ttcaacagca tgttgttgac
     3121 ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct ggcgaaccag
     3181 gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc cgagatcgct
     3241 cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa tcagcgccac
     3301 aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca tgttggcaac
     3361 atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca gataaggaat
     3421 attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac gatagccgaa
     3481 accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc gcgtttcaac
     3541 atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc caccgtgttt
     3601 cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc cgaccatacc
     3661 gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc attcggacag
     3721 atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca gcccctgaga
     3781 tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa taaccgcttt
     3841 ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta cagcgcgatc
     3901 tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt ttacagagtg
     3961 ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca gcgtcaggtg
     4021 cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag cttcgaggaa
     4081 cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta cgtcatgacc
     4141 ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa taacctgcac
     4201 ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt agatacgacc
     4261 cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt agtgccccag
     4321 gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt ggattgggct
     4381 catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca cattgaggcc
     4441 acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca gagaggatac
     4501 gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa gttaggttga
     4561 aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc tgtgccacaa
     4621 actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga gaaaatagcc
     4681 cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa atgacacaaa
     4741 tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac ctgttgccag
     4801 acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc ggcaacaata
     4861 cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa tgcgatttct
     4921 gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc ccccatttcc
     4981 tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac ctcgtcgatc
     5041 tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc aacgttggcg
     5101 caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg aatatcgtcc
     5161 agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc ccaacgggca
     5221 agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg gcgacgctct
     5281 aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc ttcctgagcc
     5341 atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg aagatcgtca
     5401 aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt gtaaggcgca
     5461 atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc gcgcaggcgc
     5521 tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa attgcgggtc
     5581 tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg ccatatccag
     5641 ctatgtaaga ctaactcccg tacaaccgac gcgcgtcgat aacatccggc acctggttga
     5701 gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc atgtcgatgg
     5761 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5821 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5881 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5941 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     6001 gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     6061 tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct ttactgcggt
     6121 tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc tgctcttcgg
     6181 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     6241 cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc ggcagcagat
     6301 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     6361 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     6421 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     6481 gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag gtgaacggca
     6541 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     6601 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6661 agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt tcgccggaat
     6721 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6781 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6841 actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacagtt tttccacccg
     6901 gccccagaac cacagtatga atagactgat aaccgtttgg tttcgggtta gcgacgtaat
     6961 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     7021 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     7081 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cgaccataca
     7141 cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca aactcttcga
     7201 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     7261 tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     7321 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acacgttcat
     7381 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacta
     7441 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     7501 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     7561 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctgacta
     7621 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7681 atgtcgagag gatctccacc atctcaacac cacgccacaa taacaggctg gcatccggat
     7741 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7801 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7861 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7921 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7981 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     8041 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     8101 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     8161 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     8221 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     8281 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     8341 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     8401 cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     8461 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     8521 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     8581 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     8641 ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc tgcccagaca
     8701 tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc attgtttgac
     8761 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     8821 taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat cggcgatcac
     8881 ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8941 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     9001 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     9061 tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg ggataaataa
     9121 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     9181 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     9241 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     9301 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     9361 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     9421 ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     9481 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     9541 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     9601 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9661 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9721 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9781 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc tgcgtgatga
     9841 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9901 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9961 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
    10021 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
    10081 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
    10141 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
    10201 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
    10261 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
    10321 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
    10381 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
    10441 acgataaagg gttggaactg acgcttaata ttaaaagcga cgtgcctgat aacgtgatcg
    10501 gcgacccact gcgattacag caaatcatca ctaacctagt ggggaatgca attaaattca
    10561 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
    10621 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10681 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10741 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10801 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10861 ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10921 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10981 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    11041 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    11101 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    11161 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    11221 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    11281 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    11341 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcag
    11401 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    11461 tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga caaacgccgg
    11521 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    11581 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    11641 acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11701 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11761 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11821 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11881 atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag ctgatcgaac
    11941 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    12001 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    12061 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    12121 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    12181 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    12241 aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    12301 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    12361 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    12421 acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc aaacggttct
    12481 ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc agtaatttta
    12541 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    12601 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12661 tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa cgagagcgta
    12721 tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag gccagtcgcg
    12781 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12841 tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc gttcagcgac
    12901 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12961 atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    13021 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    13081 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagaggt
    13141 gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    13201 tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag cagatccagc
    13261 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    13321 ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    13381 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    13441 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca
    13501 atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    13561 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    13621 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13681 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa ggcagacgtt
    13741 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13801 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13861 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13921 ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg cagccccagg
    13981 tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc gtccagaagt
    14041 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    14101 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca
    14161 tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    14221 catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca ccgttgggat
    14281 caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg tcgatttctt
    14341 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    14401 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    14461 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    14521 ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    14581 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    14641 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtctcaaaat
    14701 cggctgcctg atttccttta tgtacctgct gaaccacttt attcaggcgc gcaacttcct
    14761 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14821 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg cgggtgaagt
    14881 atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg accggaatga
    14941 ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc cgcgacaggt
    15001 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattagg
    15061 gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc aacgatataa
    15121 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa ctggccaccg
    15181 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    15241 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    15301 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    15361 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc acccagcaca
    15421 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    15481 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    15541 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    15601 atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg atccatattg
    15661 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15721 tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag accgcccata
    15781 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15841 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15901 gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15961 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    16021 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    16081 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    16141 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    16201 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    16261 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    16321 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    16381 cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    16441 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    16501 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    16561 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    16621 gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc cagtaaaaat
    16681 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16741 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16801 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16861 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcaaaa
    16921 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16981 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    17041 ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt cgcttaattc
    17101 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    17161 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    17221 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    17281 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    17341 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    17401 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    17461 tggcgtaaat ctccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    17521 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    17581 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    17641 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17701 tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17761 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17821 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgaac agtaaaaaca
    17881 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17941 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    18001 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    18061 cagccagaat aagcgcgcgt tgcggatggt ccgtcgctag cgccatttca taatagggtg
    18121 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    18181 tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    18241 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    18301 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    18361 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    18421 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    18481 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    18541 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    18601 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18661 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18721 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18781 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18841 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18901 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    18961 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    19021 gcatccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    19081 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    19141 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    19201 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    19261 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    19321 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    19381 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    19441 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    19501 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg cttagtttat
    19561 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    19621 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19681 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19741 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    19801 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19861 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19921 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19981 ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt gatgaccagg
    20041 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc accagcggtg
    20101 aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    20161 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    20221 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    20281 aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc gtttacgcac
    20341 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    20401 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    20461 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    20521 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    20581 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    20641 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20701 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20761 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20821 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataata
    20881 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20941 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    21001 ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaaccagc
    21061 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg gaagcgccga
    21121 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    21181 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    21241 tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    21301 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    21361 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    21421 actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac gcgcgccgcc
    21481 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    21541 cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac tggtcaatgc
    21601 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21661 agct
//
LOCUS       DAJTRQ010000059        21550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000059 DAJTRQ010000000
VERSION     DAJTRQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..753
                     /locus_tag="OO332_002305"
     CDS             <1..753
                     /locus_tag="OO332_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5942761.1"
                     /translation="SGIGLAIVEELLAQGANVQMVDIHGGDGQYEGHKGYQFWPTDIS
                     STKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAPAGQYELNEAAFEKMVNIN
                     QKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKE
                     LGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQLREGYTKNAIPIGRAGRL
                     AEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            763..1170
                     /locus_tag="OO332_002306"
     CDS             763..1170
                     /locus_tag="OO332_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5942762.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1170..1664
                     /locus_tag="OO332_002307"
     CDS             1170..1664
                     /locus_tag="OO332_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5942763.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            1730..2527
                     /locus_tag="OO332_002308"
     CDS             1730..2527
                     /locus_tag="OO332_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5942764.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2538..3326
                     /locus_tag="OO332_002309"
     CDS             2538..3326
                     /locus_tag="OO332_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5942765.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3378..4607
                     /gene="sorE"
                     /locus_tag="OO332_002310"
     CDS             3378..4607
                     /gene="sorE"
                     /locus_tag="OO332_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5942766.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            4989..5678
                     /gene="pepE"
                     /locus_tag="OO332_002311"
     CDS             4989..5678
                     /gene="pepE"
                     /locus_tag="OO332_002311"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5942767.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5769..7400)
                     /locus_tag="OO332_002312"
     CDS             complement(5769..7400)
                     /locus_tag="OO332_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5942768.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7620..11303)
                     /gene="metH"
                     /locus_tag="OO332_002313"
     CDS             complement(7620..11303)
                     /gene="metH"
                     /locus_tag="OO332_002313"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5942769.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11503..12327
                     /gene="iclR"
                     /locus_tag="OO332_002314"
     CDS             11503..12327
                     /gene="iclR"
                     /locus_tag="OO332_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5942770.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12644..14828
                     /gene="espL4"
                     /locus_tag="OO332_002315"
                     /pseudo
     CDS             12644..14828
                     /gene="espL4"
                     /locus_tag="OO332_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(14797..16533)
                     /gene="aceK"
                     /locus_tag="OO332_002316"
     CDS             complement(14797..16533)
                     /gene="aceK"
                     /locus_tag="OO332_002316"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5942771.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16717..18021)
                     /gene="aceA"
                     /locus_tag="OO332_002317"
     CDS             complement(16717..18021)
                     /gene="aceA"
                     /locus_tag="OO332_002317"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5942772.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18051..19652)
                     /gene="aceB"
                     /locus_tag="OO332_002318"
     CDS             complement(18051..19652)
                     /gene="aceB"
                     /locus_tag="OO332_002318"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5942773.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(19921..20850)
                     /gene="metA"
                     /locus_tag="OO332_002319"
     CDS             complement(19921..20850)
                     /gene="metA"
                     /locus_tag="OO332_002319"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5942774.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21007..21447
                     /locus_tag="OO332_002320"
                     /pseudo
     CDS             21007..21447
                     /locus_tag="OO332_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         5285 a         5541 c         5467 g         5257 t
ORIGIN      
        1 tccggtattg gtctggcgat tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg
       61 gtcgatattc acggtggcga tggtcaatat gaaggtcata aaggctatca gttttggccg
      121 accgatattt ctagcacaaa agaggtaaat catacggtag cggaaattat ccagcgtttt
      181 ggtcgcatcg acggtctggt caataacgcc ggggtcaatt tcccgcgtct gctggtcgat
      241 gagaaagcgc ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat
      301 atcaaccaga aaggtgtttt tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa
      361 catgatggcg tgattgtgaa tgtttcctca gaaagtgggc tggaaggctc agaaggacaa
      421 agctgttacg ccgctaccaa agccgcgctc aatagcttca cgcgctcctg gtcgaaagag
      481 ctgggtaaac acggtatccg tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg
      541 ctacgcacgc cggaatatga agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag
      601 ctgcgtgaag gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa
      661 gtagctgatt ttgtttgtta tctgttgtct gaacgcgcca gctacatcac cggagtaacc
      721 actaacattg cgggcggcaa aacgcgcggc taaggaggct ttatggtcaa cgccattttt
      781 tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac
      841 gccaatgttg aagccgttga atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag
      901 ctggaaaagt tagcaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag
      961 tgcggcagcc cgtggaatgc ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta
     1021 atcagcggcc tttctctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat
     1081 gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa
     1141 caactggcaa cgacagagga ggatttctga tgaacattac gctcgcccgc attgatgacc
     1201 gtctgatcca cggccaggtc actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta
     1261 ttatctgcaa tgacgaagtt tataacgatg aagtgcgccg gacattattg cgtcaggctg
     1321 cgccaccggg aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg gtttatcaca
     1381 acccgcaata tcaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag
     1441 cgatggttcg ccagggggta aaaataggca ccctgaatat tggcggcatg gcctggcggc
     1501 ccggtaaaaa acagttaacc aaagcggttt cattagatga tgatgatatt aacgcatttc
     1561 atgagttaaa taatcttggt gtcatactgg atttacgcgt cgtagcgtca gatccctcaa
     1621 tcaatattat cgacaaaata aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt
     1681 acatatgctt taacaggcag ggatgacgac acttaaaagg tgacaaatca tggaaataag
     1741 taccctacaa atcattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct
     1801 ggatgaattt cagactcatc gcccgttaat tgcctgtacg gtgattggtt taattctcgg
     1861 tgatttaaaa accggaataa tactcggcgg tacgctggaa ttgatagctc tcggctggat
     1921 gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct
     1981 ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc
     2041 ggcaggccag gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc
     2101 ggataaagca gcagaagagg cgcggtttcg cactctcgat attctgcatg tctccgcgct
     2161 tggcgtgcag gcgctgcgcg tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc
     2221 cgatatggtc agcaatatgt taaacgccat tcccgaattt gtgacccgag gactacagat
     2281 tgctggcggt tttatcgtgg tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa
     2341 atatttgatg cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagcct
     2401 gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc taaatccaca
     2461 gtggcgtaaa gctgaacctc aactccaggc caccgcctcc accgcccttg accaacttga
     2521 cgattaacgg agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt
     2581 ttctgcgctc caacctgcaa caggcgtcct ttaactttga acgtattcac gggctgggct
     2641 tttgctacga catgatcccc gccatcaagc gactttaccc attaaaagag gatcaggttg
     2701 cggcgctcag gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca
     2761 tcggcgcgga aattgatgac ggtaccatca acggcatcaa agtcggtctg atgggaccat
     2821 tggcaggagt tggcgatcca ctggtctggg gaacgctgcg cccgattacc gccgcgctcg
     2881 gcgcatctct ggcactttcg ggcaacattc tcggcccgct gctgttcttc tttattttca
     2941 acgcggtgcg tctggcgatg aagtggtatg gcctacagct cggctttcgc aaaggggtga
     3001 atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc
     3061 tcggactgtt tgtgatgggc gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg
     3121 tggtttcaca aacgcatgcc gccgatggct ccaccgtcac catgaccgtg cagaacattc
     3181 tcgaccaact ttgccctggt ttgctggcgc tcggtctgac gctactaatg gttcgtctgc
     3241 tcaacaaaaa aattaacccg gtatggctga ttttcgccct gtttggctta gggattatcg
     3301 gcaatgctct gggcttcctg tcctgattct tcgccccggc acgactgccg aggccatcgc
     3361 tcaacatgag gtggtttatg aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac
     3421 gcctggaaac ctttgacctt cctgaaatgc aggaggatga aatcctcgca acggtggtca
     3481 ctgacagcct gtgcctctct tcctggaaag aggccaatct gggtgaaaac cataaaaaag
     3541 tacccgacga tgtggcgacc aacccaatca tcatcggcca cgaattttgc ggcgatattc
     3601 tggccgtggg taaaaagtgg cagcacaaat tccagccggg tcagcgttat gtgattcagg
     3661 ccaacctgca actccccgac cgcccggatt gccccggcta ctccttcccg tgggtaggcg
     3721 gcgaagcgac gcatgtggtt attcccaacg aggtcatgga acaagattgc ctgctggcat
     3781 acgacggcga aacctatttt gaaggctcac tggttgaacc gctttcctgc gtgattggcg
     3841 cgttcaacgc caactatcat cttcaggaag gtagttataa ccacacgatg gggattcgcc
     3901 cgcaagggcg cacgctgatc ctcggcggca ccggaccaat gggactgttg gcgattgatt
     3961 atgcgctgca tggaccagtt aacccgtcgc tgctcgtcat taccgatacc gacaacgaca
     4021 aattgagtta tgcgcgcaag cactatccat cagaaccgca aacactgatt cattatctca
     4081 atgccaccga tgcagcattt gatacgttaa tggagctgag tggcggtcac ggcttcgatg
     4141 atattttcgt ctttgtgcct aatgaaggac tggtgactct cgcctcttcc ttgctggcga
     4201 cagatggttg cctgaatttc ttcgccggac cgcaggataa acatttcagc gcgccaatta
     4261 atttctacga tgtgcattat gcatttaccc actacgtggg cacgtcaggc ggcaataccg
     4321 acgacatgcg cgcggcggtc aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg
     4381 taacacatat tcttgggctg aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg
     4441 gcggcggcaa aaagctggtg tataccggga aatacctgcc gctgacgtca ctcacgcaga
     4501 ttcaggatca agcactggcg gcgattctgg cgcgtcatca ggggatctgg tccggtgagg
     4561 cggagcaata cctgctcgcc catgcagagg caatttccca tgattaatcg cgctacttaa
     4621 ctctgcatgt cgctggctgg tcgccccagc aacttcgcga ccggatgtgc ggctgattgc
     4681 tgaagcagca caatttgcac gagaaggata aaaaatatca gcgccttaag gggcgctcaa
     4741 attggtggca aagtacacat tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac
     4801 ataattttga aaatccaata tattgcagtt atctggtagg ccaggtaagc gtagcgcatc
     4861 aggcaatgct gtgtttgtca tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc
     4921 atgtcagtaa gtggttaaat tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg
     4981 gagaagtaat ggaactgctt ttattgagta actcgacgct gccgggtaaa gcctggctgg
     5041 aacatgcact gccgctaatt gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc
     5101 ctttcgctgg cgtaacgcag acctgggatg attacacaga gaaaacggct gcggttctcg
     5161 ctccgctggg tgtttctgtc accggtattc atagcgttgt cgatcccgtt gccgcgattg
     5221 aaaatgcgga aattgtgatt gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc
     5281 gcgagcgcgg gctgctggct ccaattactg acgtggttaa acgtggcgcg ctgtatattg
     5341 gctggagcgc aggcgctaac cttgcttgcc caaccattcg taccaccaac gatatgccga
     5401 ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc gttgcaaatc aacccgcact
     5461 tcaccaacgc gctgccggaa ggccataaag gtgaaacccg tgagcagcgt attcgcgaac
     5521 tgctggtcgt cgcgccagaa ctgacgatta ttggtctgcc ggaaggtaac tggatcacag
     5581 tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg
     5641 aagaagcggt tccgctggaa gctggtcacc gtttttaagt gaattgccgg atgcatgatc
     5701 cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg tcttatcagg cctacaggat
     5761 tcacaatatt aatactcgtc ccgtccttca tcttcatccg gctgttccag cacactgtaa
     5821 gccaccgaac aaaacagcga gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc
     5881 gaactggttt caatgctctg cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa
     5941 tagcggcgat taagaatgcg aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg
     6001 acatcgccag agaagaacac cgacattgcc agctttaaat tactgagcaa ttgctcatag
     6061 agcgcatcca gttccttcaa cccatcaagc gaaaatgccc gccgtgctgc cagcgattta
     6121 tcggcaattt cgctgcccat gcgctcgacg atatcggagg cctgttcaag gttgagcgac
     6181 atttcgatga tctccgccca gcggcgcgac tcttcctccg ccaactcttc ttttggcatc
     6241 cgcgccagat acagcttaat ggcggtgtag agaacgttga tatcatctgc cagcttacgc
     6301 agctctttct cctgccgtgg ctcgccgtgc atcactttat tcagcccttc catcatctgt
     6361 tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc
     6421 gtgtccagcg cgctgacatc cagatgttta ggccgtagct gggtatccag ttccggttca
     6481 tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa caaatggcag catgaccagg
     6541 caacgtacaa ggttgtagaa gacgtggaaa tagatcacca gttccgcttt tggcaatgac
     6601 agcttcccca ttgtctctgc cagcaaatgg acaaacggca ggataatcag gctacccacc
     6661 agcttaaaca gcagactacc cagcgcgaca cggcgggctg cggcattggc ggcactgttg
     6721 ttgagcatcg ccagcaggcc ggaaccgagg ttagctccaa tcaccagaca gagcgccacg
     6781 gggaaggaga taatgcctgc agcggtcaga gtcgcggtca gcagtacagc agcaaggctg
     6841 gagtagctga taatggcgaa catcgcgcca atcagcgcat ccagcagaat atcgccggtc
     6901 agcgaggcaa agatcacctg aacgccgttt gcctgggtga tcggcgttac ggcctgcaca
     6961 atcaactcca gcgccagcaa aatcagccca agaccaatac cgacgcggcc cagttgcccg
     7021 gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag taacggtgac
     7081 agccaggata agtcgaaggt gagaatacgc gccattagcg ccgtcccgac atctgccccc
     7141 agcacaatga ccagagccgg tgcgagggct accagatcct gagcgacgaa cgaggtcacc
     7201 agcatggtgg tggcattact gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag
     7261 gcgagcggct ttttttcgac gctccggcta aggacagtac gcaaacgcgc gccgaagacg
     7321 cgcattacgc cggttcgaac aatatgagtc ccccagacca gcagggcgac ggcagaaagc
     7381 aggtgaagca gcgttaacac gagttcaggt tctccttatc gttatatgtt cctgaagcaa
     7441 ttgaatgacc aaaggaccgg aaacacgctg actggcatct ttactgtgcc ttttgacatt
     7501 cgtttttccg gctaagatta ttccagcctg tttccagtat aagtgtttca tattaataaa
     7561 gagacagggc actcattgag cgccctgttt gttgtaagga aaagtgacag atttgtgaat
     7621 cagtccgcgt cataccccag attcggtgcc agccagcgct caacatcgga gacgctcata
     7681 cctttacggc gggcatagtc ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac
     7741 ttgctgtccg gatgactgaa ataccaaccc gaaaccgatg caccaggcca catagcgaaa
     7801 gattctgtga gtttcatgcc agtatgtttt tccacttcca gcagctccca gatggtggct
     7861 ttttccgtat gttccgggca ggccggatag cccggtgccg gacggatgcc ctggtagttt
     7921 tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg catagcccca gtagacttta
     7981 cgcacacgct catggagata ctccgcaaag gcttcggcta aacggtcggc aagcgctttc
     8041 accatgattt tgttgtaatc atcgtgctgc gcttcaaagg catcagccag tgcgtcctct
     8101 tccagcccgc cagtcacggc aaatgcgccg atgtaatctg ctttaccaga aagcttcggc
     8161 gcaacgaagt cagcgagaca gtagttagcg aatcccgttt tttcggtctg ttgacgcaga
     8221 tggtggctga cgttgatcac atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg
     8281 cccacacggt ttgccgggaa caggcccacc acgccacgcg gattcagcgt tttctcggcg
     8341 cttaatttat ccagcatgtc gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc
     8401 acttcatctt ccagaatgcg cggatacttc ccggccagcg accaggtcat aaagaacggt
     8461 gtccagtcga tgtaattacg cagcgtttcg atgctggctt ccacttcctg cacgccgaga
     8521 cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc
     8581 gcttccagcg tgaccggtgg tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt
     8641 tcgtactcct tgcaggtacg agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc
     8701 gctaccacac caacggtgcg cgaggcattc tgcacataca ccgtagggcc gctgtagttc
     8761 tgctcgattt tcaccgccgt gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga
     8821 atggtgaagc cctgacgctc catctctttc gccacgttaa ccatctcgtc cagcgacggc
     8881 gtgataagcc ccgaaaggcc aatcaaatca gcattcactt ctttggcgat acggagaatt
     8941 ttttccgcag gcaccataac gccgagatcg acaatttcgt agttgttaca ttgcagcacc
     9001 acaccaacga tatttttacc gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc
     9061 ttgccgttgg ttttaccctg ctctttgctg gcttcaataa acggttcgag atacgccact
     9121 gcctgtttca tgacgcgcgc cgatttgacc acctgtggca ggaacatttt gccttcgcca
     9181 aacagatcgc cgaccacgtt catgccgtcc atcaacgggc cttcaatcac ttcaatcggg
     9241 cgcgtagcct gctggcgggc ttcttcagta tcctgctcga taaactcggt tatgcctttg
     9301 accagcgagt attccagacg tttattcacg tcccacgagc gccactccgc ttgctgggcg
     9361 ttagcagtgt cgtccgcttt actgccgcga tatttctcgg caagctccag taaacgctca
     9421 gtgccatcgt cacgacgatt aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt
     9481 aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc ccatatccat gccattgcga
     9541 atagcgtagt agaggaacac tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat
     9601 gagaaagaaa cgttagatac gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg
     9661 tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt gctcttcaat accggtggcg
     9721 accgcgaaga tgttcgggtc gaagatgata tcttctggcg ggaagccaac ctcttcggtg
     9781 aggattttgt acgcccgacg gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc
     9841 tgttcgtcaa aggccatcac caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga
     9901 tggataaagg cgtcgacgcc ctctttcatc gagatagagt taacaatgcc tttgccctgg
     9961 atacacttca gacctttttc aatgacatcc cattttgagg agtcgatcat aatcggcacg
    10021 cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca
    10081 tcgagcatcc cttcatccat gttgatatcg ataatctgcg cgccgttttc cacctgctga
    10141 cgcgccacat ccagcgcctc gctgtatttc tcttctttga tcaggcgctt gaacttagca
    10201 gagccggtga cgttggtgcg ttcacccacg ttcacaaaca ggctatcatc gccaatgttc
    10261 agcggctcca ggccagacaa acggcaggct acgggaattt ccggcagttt gcgcggcgct
    10321 aatccttcta ctgcacgact catcgctgca atatgttgcg gcatggtccc gcagcaacca
    10381 ccgacgatat tgagaaagcc cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg
    10441 gcgtcgagat cgtattcacc aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg
    10501 tagcattccg caatccgtga tagttcctgc acgtactggc gcagttcatc gggccccagc
    10561 gcacagttca ggccaaaggt cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg
    10621 gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga tggtgccgga gatcataatc
    10681 ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg caaagaccgc cgctttggcg
    10741 ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg caccaccttc caccagcgct
    10801 ttggtggatt cgcgataggc cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga
    10861 tcgttgacgt ccgaagaaat agacgccgtg cggttggttg ggccgagaac accggcaacg
    10921 tagcgcggtt tctccggcgt gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc
    10981 gccgccgcaa agttgatttc cgccgacagg gattccatct ggtaatccac catcgcaatg
    11041 gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg
    11101 tggatagcgg cgatcacttc cggtttactg agtaccagca ggtcgttgtt gcctttgagg
    11161 tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc
    11221 tggatcatgg tgcccatacc gccgtccaac accagaatac gttcatttaa ctgcgcacgc
    11281 agttgttcca ctttgctgct cacacttgct cccgacacgc tcaacccaga tttaataaaa
    11341 attcaacaaa ccatactggc ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg
    11401 agatttgttc aacattaact catcggatca gttcagcaac tattgcatta gctaacaata
    11461 aaaatgaaaa taatttccat gatacagaaa aaggaggctg tcatggtcgc ccccattccc
    11521 gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag cgactggaca ggttcagtct
    11581 ttaacgcgtg gcctgaaatt actggagtgg attgccgaat ccaatggcag cgtggcactc
    11641 acagagctgg cgcaacaagc cgggttaccc aattccacga cccaccgcct gctaaccacg
    11701 atgcaacagc agggtttcgt gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca
    11761 catgccttta tggtcggcag cagctttctc cagagccgta atttgttagc aattgttcac
    11821 cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat
    11881 caaagcgatc acgaagcgat tattatcgac caggtacagt gtacccacct gatgcgaatg
    11941 tccgcgccta tcggcggtaa attgccgatg cacgcttccg gtgcgggtaa agccttttta
    12001 gcccaactga gcgaagaaca ggtgactaag ctgctgcacc gcaaagggtt acatgcctat
    12061 acccacgcaa ctctggtgtc tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa
    12121 cggggttatt catttgacga tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt
    12181 attttcgatg agcaccgcga accgtttgcc gcaatttcta tttccggacc gatttcacgt
    12241 attaccgatg atcgcgtgac cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg
    12301 acgctggcgt acggtggaat gcgctgactt tttctggcgg gcagaggcaa tattctgccc
    12361 atcatacctg agtggcaata gaataagggt gtctgttaat cgcattgacg ccaaaataac
    12421 ttaatgtcat acacttcaca cacttttatt aactcagcat tatttttaaa catcaaacca
    12481 cttaattatt aacaaaatcg caagatctca ttccaccgaa acttttacgg ctgcattatg
    12541 agattgagcg atttatgata tttttttagt tgtaatcata ttaaattttt tcactgttct
    12601 gatagttaaa attcaagaca tcaataaaca atgagatatt taaatgatta ctcgtattcc
    12661 tcgtagttct ttctctgcaa atattaataa tacagcccag acaaatgaac accaaaccct
    12721 gagtgaattg ttttataaag aactcgagga taaattttct ggcaaggagc tggcgacgcc
    12781 tctattaaaa agcttctcag agaactgtag acaaaatgtt cgtcatatct ttagcaacaa
    12841 ggattttgtc attaaatttt ccacgtccgt cttacaagct gataagaaag aaattacgat
    12901 aattaataaa aacgaaaaca cgacactcac tcaaaccatt gctacaatat ttgaagaata
    12961 cctaattgaa attttacctc aacgctcaga cactcttgat aaacaagaat taaacctaaa
    13021 atcagataga aaagaaaaag aattcccaag aattaaactt aatggtcaat gttattttcc
    13081 ggggcgcccc aaaaccgtat agtataccga cacattgctg cacaatatat taatgatatt
    13141 tatcagaatg ttgattacaa accccatcaa gatgattact cttcagctga aaaatttctc
    13201 actcacttca acaaaaaatg caaaaaccag actttggcgt tgatttccag ccgtcctgag
    13261 gggcgttgcg ttgctgcctg cggtgatttc gggctagtta tgaaagcata ttttgacaag
    13321 atggaatcaa atggcatcag tgttatggca gccatattac tggtggataa ccatgctttg
    13381 acggtccggc taagaataaa gaacacaact gaaggatgta cccattacgt gatttcggtt
    13441 tatgatccta atgtaactaa cgataaaata agaattatga gcgaaagcaa agaggatatt
    13501 aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa atggtcaaat
    13561 gatcatgtta ttaaccaatc tgttgcaata attccagcac ttccgaaaga acagctattg
    13621 atgttaaaag gaactgtgga tgaaataacc cctccattat caccagcaac gatgaatttg
    13681 ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcagct ccagaaaatg
    13741 ccagaactac atagaacaga aatgttgact gcctataata gtattaacct ccccggttta
    13801 tatttggcta taaattatgg caatgcggat atcgttgaga ctattttcaa ttcattgtca
    13861 gaaacaggat atgaagggtt actctcgaaa aaaaatctca tgcatattct ggaggcaaaa
    13921 gataaaaatg gtttttctgg attattttta gcgatatcac gtaaggataa aaatgttgta
    13981 acctcgattc tgaacgtctt acctaaactg gccgcaacac atcatttaga taatgaacag
    14041 gtgtataaat tcctgagtgc caaaaataga acgtccagcc atgttttata ccatgttatg
    14101 gcgaatggtg atgccgacat gctgaaaatt gttttggtcg cgttaccttt gttaattcgc
    14161 acatgtcatt tgactaaaga acaggtactc gatctcctga aggcaaagga tttttatggt
    14221 tgcccaagac tatacctggc gatgcaaaat ggacatagct atatcgtgaa agttattctc
    14281 gaagcattgc cctgtctggc ccaggaaatt aacatttcag cttccgatat tgtagatctt
    14341 ctgaccgcta aaagtcttgc gcgcgacacg ggtttgttta tggccatgca gcgcggacat
    14401 atgaacgtta ttaatactat ttttaacgca ttacccactc tttttaatac gtttaaattc
    14461 gataaaaaaa tatgaagccc ctcctcctgg caaataattc taatgaatat cccggtttgt
    14521 tttcagcgat acagcataaa caacaaaacg ttgtagagac ggtttatctt gctttatctg
    14581 accatgcacg cctgtttgga tttaccgctg aagatattat ggatttttgg caacacaaag
    14641 cgccacaaaa atactctgcc tttgagttgg cttttgaatt tggtcaccgg gttattgctg
    14701 aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg gataaccctc
    14761 gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct ccccataccg
    14821 tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc cttcacgtat
    14881 gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa
    14941 cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg ggaaaacatc
    15001 gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat agcgcggcgg
    15061 cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac
    15121 cacacgcccg tgacgggtga caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt
    15181 agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc gcaactgctg
    15241 accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga tatagagatg
    15301 gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct ggagcagtaa
    15361 ttccattaat accggggaaa tatgccgctt ctccagcaca aagttttcaa actcctgggt
    15421 gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg
    15481 agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga atacccgatc
    15541 aaagcccggc agcgtaaaca ccaacatcac cattccacga atacccggcg cttcaatgaa
    15601 ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg ttttggcgtg
    15661 cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca gaatttcccg
    15721 tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa
    15781 gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc
    15841 gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt
    15901 gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct gtaaatgact
    15961 ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc
    16021 acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag
    16081 agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa
    16141 gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg agcggtggtc
    16201 aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc gcgggtaatc
    16261 cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg
    16321 gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat cgtaaagatg
    16381 gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga aacgctgctg
    16441 cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt
    16501 ttgagcaatc agtaattcca ggccacgcgg catttagcgc actcatcagg agcagagaat
    16561 tgccggatgc ggcgtaaaca ccttatccag cctacgttcg gcaacggttg taggcatgat
    16621 aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc
    16681 ttatccggcc tacagtcagc aacgtttgta ggttgcttag aactgcgatt cttcagtgga
    16741 gccagtcagc gcggtgactg aagacgtgcc gccctgaata atagtcgtca ctttatcgaa
    16801 gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt tcgcggcggc
    16861 aaattccggc tgctgcactt tctcaacata gtgcttcata ctttcgccct gcgcataagc
    16921 gtttgccagg tcgaacatgt taaaccacat gctgtggata cctgccaggg tgatgaactg
    16981 gaacttgtag cccatatccg acaactgctg ctggaagctg gcaatagttt tgtcgtcgag
    17041 gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc ccggatattt
    17101 cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg aggtttcaca
    17161 ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc
    17221 cgcatgagta cggaagaagc cttcactggt acgctcgccg gtaataaatt cgctgtcata
    17281 cgggtcgcaa tctgaggtga tcagatccgc cgcatcagca tcggtacgtg caaccagcag
    17341 ggttggaacg cccgtcacgt cagctgccag acgcgccgcg accagtttct gaatagcttc
    17401 ctgagttggc actaaaactt tgccgcccat gtgaccgcat ttcttcactg acgccagctg
    17461 atcttcgaag tgaactgccg cagcaccggc ttcaatcatc gctttcatca gttcaaaggc
    17521 attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga agtaatcgac
    17581 atagcgcgga tcgcccggct caatgcccac ggaccattgg atctgatcgg cacgacggaa
    17641 ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga gcgactgatc
    17701 cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg acagatagac
    17761 tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg caccgaggct
    17821 gttgatgtag ccttttttcg actcaccgtg cagcagacgc cacattttcg ctgcgcccag
    17881 ttgcgccagc gtgcattcag gattgactga accgcgtaat ttcaccacat cttccgcact
    17941 gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta attcttcaat
    18001 ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt ttacgctaac
    18061 aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt gatctgttcc
    18121 atcaagcgtg cggcatcgtc aaaacggccc tgggagaaac gttcttcgcc cagttcgctg
    18181 gcaatgactt tcatctcttc gcccagcatc tggcggaaca aggctttggt caccggtttg
    18241 ccattgctca acgttttttg atgatggatc cactgccaga tcgaggtacg ggaaatttca
    18301 gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc ggagatccac
    18361 gcttcgatgt actgcacagc cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca
    18421 ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact
    18481 tcaagctgat ttttacggga gccgagaata tcgttgaata ccgccattgc cgtgtccgca
    18541 aggcctgggt gagcgatcca tgtgccatcg tgaccgttat tggcttccag cgctttatcc
    18601 gcttttactt tgttgagcac ctggttatta cgctcttcat ctttgctcgg aataaacgct
    18661 gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcag caggcgtgag
    18721 taagcattca ggaatggttt atccatcgtc acggcctgtc tgtctggcag gacacgatcg
    18781 ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccagcgacc gcagttcaga
    18841 ccaacaatat ggtcgcgcag cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc
    18901 gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg atcttctgca
    18961 tagctgaaga cttcgctcca ccaggccgct tcctgccagg actgggtttt cggcagatag
    19021 aaatagggac cgcagccctt tgccaacagt gcctgatagt tgtggaagaa atagagcgca
    19081 aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt ttccggcaag
    19141 tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg ataaattttg
    19201 cctgcttcat tggtatagct gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg
    19261 tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat aaagactttc
    19321 acattggcgt tcagtgcgtt gatcaccatc ttgcgctcta ccgggccagt tatctctacg
    19381 cggcggtctt ctaagtccgc aggaatcccg cgaattttcc aatcagcatc gcgaatggaa
    19441 gccgtttccg aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg
    19501 cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat
    19561 tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct tgtgaaagcc
    19621 agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa
    19681 acttcccctc tgtgaacgaa ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac
    19741 tcatttaaaa tgcaaaaaca aaaacaattt ccattttaat taaaaatact tgataactta
    19801 ttgataacaa tatgaataaa atttattcac tttctgaggt gcgtttcgga aataaaaaag
    19861 gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta tcacagaaga
    19921 ttaatccagc gttggattca tgtgccgtag atcgtatggc gtgatctgat agacgtaata
    19981 gttgagccag ttggtaaaca gcaaattacc gtgactacgc cagctcgctc gcggtttgtt
    20041 ttgcggatcg ttgtgcggga aatagttata cggtacatcc gggtctagtc cggcttccac
    20101 atcgcggaaa tattcctgcg ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa
    20161 ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc ccttcttccg tctctgccag
    20221 aatttccaga tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg
    20281 cggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat ggagaatatg
    20341 atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat
    20401 attgagcgcg gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt gatctttcga
    20461 ccactccagc acctgtttga tctgcggcca gtaagcgaca tcattaaact ccaccaggcc
    20521 cagcggcgca ccggttacaa tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt
    20581 acagtagaag ttgttcagat gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg
    20641 caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa actgattttc
    20701 agtttcaatc ttcttcggca tcaggttaag gatcagaacc ttaagtggac gaatttcctg
    20761 accagacgca cgagaagttg tcatcacaaa cacgttttct tcacgcaaga aattgacggc
    20821 gggtagctcg tccggcacac gaatcggcat aacctgatta cctcactaca tacgcttata
    20881 cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg agcctgcatg
    20941 ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa tataagaatg acgaaaagga
    21001 gagaaaatgg ttattagtat tcgccgctca cggcatgagg aaggggagga actcgttgcg
    21061 atttggtgtc gatctgtcga tgccacccac gattttttat cagcagagta tcgggccgag
    21121 ctggaagagc tggtgcgttc ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa
    21181 cgggagcagc cggttgggtc tatgttgtta agtgggcagc atatggatgc gctgtttatc
    21241 gatcctgatg tgtgcggctg cggcgtgggt cggatgctgg tggagcatgc gctctcgatg
    21301 gcaccggaac tgacaaccaa cgttaatgag caaaatgagc aggcggttgg gttctataag
    21361 aaggtgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa accgtatccg
    21421 ttgctgaatc tggcgtatgt gggggataag ccgcccctgc cgtgcctgcc caagctggag
    21481 aagacgcgcc agcgtcgcac ccggcatttt tttcaccaac gcaaaaaggc catcctgacg
    21541 gatagccttt
//
LOCUS       DAJTRQ010000060        21520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000060 DAJTRQ010000000
VERSION     DAJTRQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(23..1021)
                     /gene="fbp"
                     /locus_tag="OO332_002321"
     CDS             complement(23..1021)
                     /gene="fbp"
                     /locus_tag="OO332_002321"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5942775.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1197..2570
                     /gene="mpl"
                     /locus_tag="OO332_002322"
     CDS             1197..2570
                     /gene="mpl"
                     /locus_tag="OO332_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5942776.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2653..3204)
                     /locus_tag="OO332_002323"
     CDS             complement(2653..3204)
                     /locus_tag="OO332_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5942777.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3298..4650
                     /gene="pmbA"
                     /locus_tag="OO332_002324"
     CDS             3298..4650
                     /gene="pmbA"
                     /locus_tag="OO332_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5942778.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4833..5219
                     /gene="cybC"
                     /locus_tag="OO332_002325"
     CDS             4833..5219
                     /gene="cybC"
                     /locus_tag="OO332_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5942779.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="OO332_002326"
     CDS             complement(5264..5728)
                     /gene="nrdG"
                     /locus_tag="OO332_002326"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5942780.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="OO332_002327"
     CDS             complement(5886..8024)
                     /gene="nrdD"
                     /locus_tag="OO332_002327"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5942781.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8418..10073)
                     /gene="treC"
                     /locus_tag="OO332_002328"
     CDS             complement(8418..10073)
                     /gene="treC"
                     /locus_tag="OO332_002328"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5942782.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10123..11544)
                     /gene="treB"
                     /locus_tag="OO332_002329"
                     /pseudo
     CDS             complement(10123..11544)
                     /gene="treB"
                     /locus_tag="OO332_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11663..12610)
                     /gene="treR"
                     /locus_tag="OO332_002330"
     CDS             complement(11663..12610)
                     /gene="treR"
                     /locus_tag="OO332_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5942783.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12795..12848
                     /gene="mgtL"
                     /locus_tag="OO332_002331"
     CDS             12795..12848
                     /gene="mgtL"
                     /locus_tag="OO332_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5942784.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12989..15685
                     /gene="mgtA"
                     /locus_tag="OO332_002332"
     CDS             12989..15685
                     /gene="mgtA"
                     /locus_tag="OO332_002332"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5942785.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="OO332_002333"
     CDS             complement(15891..16277)
                     /gene="ridA"
                     /locus_tag="OO332_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5942786.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="OO332_002334"
     CDS             complement(16350..16811)
                     /gene="pyrI"
                     /locus_tag="OO332_002334"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5942787.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="OO332_002335"
     CDS             complement(16824..17759)
                     /gene="pyrB"
                     /locus_tag="OO332_002335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5942788.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="OO332_002336"
     CDS             complement(17763..17897)
                     /gene="pyrL"
                     /locus_tag="OO332_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5942789.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18178..18573)
                     /locus_tag="OO332_002337"
     CDS             complement(18178..18573)
                     /locus_tag="OO332_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5942790.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="OO332_002338"
     CDS             complement(18705..19418)
                     /gene="bdcA"
                     /locus_tag="OO332_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5942791.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19489..20082
                     /locus_tag="OO332_002339"
     CDS             19489..20082
                     /locus_tag="OO332_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5942792.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20227..20679
                     /gene="tabA"
                     /locus_tag="OO332_002340"
     CDS             20227..20679
                     /gene="tabA"
                     /locus_tag="OO332_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5942793.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20802..>21520
                     /locus_tag="OO332_002341"
     CDS             20802..>21520
                     /locus_tag="OO332_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5942794.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5202 a         5332 c         5663 g         5323 t
ORIGIN      
        1 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
       61 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
      121 tgatatccag aatacgctct ttgccatcgc tcgctttacc acccgcttgt tccgccagga
      181 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
      241 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
      301 cgatataacg cgaggtatac gggcgattgg tggatttatc ttcttcctgg cagaatttaa
      361 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
      421 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
      481 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
      541 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
      601 ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg acagagacgt
      661 taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat tttgcgtgtt
      721 cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatcccc gcaacgatat
      781 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
      841 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
      901 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
      961 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     1021 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     1081 tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga aatgcgtttc
     1141 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     1201 gcattcatat tttaggaatt tgtggcacat ttatgggcgg tctggcgatg ctggcgcgcc
     1261 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     1321 ttgagaagca aggcattgag ctgattcagg gttacgatgc cagccagctc gagccgcagc
     1381 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     1441 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcacg
     1501 accgctgggt gctagccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     1561 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     1621 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     1681 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     1741 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     1801 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     1861 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     1921 gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa tgggaagttt
     1981 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     2041 acaatggcct gatggcgatt gcagcggctc gccatgttgg tgtagcgccg gcagatgccg
     2101 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     2161 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     2221 cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat tgctgtgctg gaaccgcgct
     2281 cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta ggtcgtgccg
     2341 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     2401 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     2461 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     2521 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcttca
     2581 gcctgatata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     2641 caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     2701 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagtgtacg
     2761 cagttgctga cgatcggcat cgggccacag attcaatact tcagcgatgg cgtcatcacc
     2821 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     2881 gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc gctggcgcag
     2941 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     3001 ctcaatagcc gggcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     3061 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     3121 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     3181 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     3241 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     3301 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     3361 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     3421 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     3481 agtgatggcg cgctggggat cactgtttat caccagaacc gtaaaggtag cgcatcgtct
     3541 accgatttaa gcccgcaggc cattgcccgc accgtacagg cggcgctgga tattgcccgt
     3601 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     3661 ccgaatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     3721 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     3781 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     3841 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     3901 gagcgtgatt acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg
     3961 gtcggggccg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     4021 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     4081 ggggcgatag cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt
     4141 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     4201 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     4261 ggcatcctga ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc
     4321 accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa
     4381 caaatgctca aagagatggg caccgggctg gtggtgacgg agctgatggg ccagggcgtg
     4441 agtgccatta ccggtgatta ttcccgtggt gcagcgggct tctgggtaga gaatggcgaa
     4501 attcagtatc ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
     4561 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
     4621 ttgccggaga tgaaaatcgc cgggcagtaa aaaactggct cgacctgccg cgctataaac
     4681 taaaaaatta cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg
     4741 agcgtactcg cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa
     4801 ggatgaagtg taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg
     4861 cagtctcctc gttggtattc agttctgcgt cgtttgccgc tgatcttgaa gacaatatgg
     4921 aaaccctcaa cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag
     4981 acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc
     5041 tcgaagataa atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc
     5101 tggtcggtca gattgacgac gcgctgaagc tggcaaatga aggtaaagta aaagaagcgc
     5161 aggctgctgc agagcaactg aaaacgaccc gcaacgccta tcaccagaag tatcgttaat
     5221 tcctcatttc cctgttgcct gcactcaggt aacagggaac ttatcatcgc aaatgatgca
     5281 ccacctgatt gctgctgccg cgccagatca gggaagggtc ttttaaatcc tgcacaaatt
     5341 tgccgtcgac cagcacgtta atcaaatcaa caacctgcat ttgcgcagcg ttgagttcgt
     5401 cgagtttata gcctgtccac acccagatgt ctttacccgg acactcggcg cggatgcgtt
     5461 ttaccagttt cagaatatcc ggcacgtttt gcggatgcag cggatcgccg ccggagaggg
     5521 aaatcccctg gcgtttgata cgagtgtcat tcagatcttt aatgatcttg tcttccattg
     5581 ctttggtaaa tggctgacca gaatttaccc gccatgtgct tttgttatag caaccggggc
     5641 attcatgaac acacccggag acaaacaggg tgcagcgagt gccggggccg ttgacgatgt
     5701 cgacaggata gtactgatga taattcatta tttcgcctgg cttatcagtc cgcgtgtagg
     5761 ctgcatccgg taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc ctacagccgt
     5821 tgccaaatgt aggccggata aggcgtttac gccgcatccg gcatttgctt agcgcagagt
     5881 gaagattaac ctatctgccc attccccaaa tgtttaacgc ggcgcttaac ttcttcctgc
     5941 ttaccagcgt taaacggacg tgcatccggg ctacctaaat atccgcacac gcggcgagtt
     6001 accgacacac gggaggcgtc atggttacca catttcgggc aagtgaagcc tttgctggtg
     6061 cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt attggtgccg
     6121 taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcttt caggttgtgc
     6181 tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg cgggtaaggc
     6241 gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcaag gtggaaactg
     6301 ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc agtatcgaga
     6361 cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt agagactgaa accataaccc
     6421 gtttcttctt tccactgatc cactgcctga cgcagacgtt caacaatggc gatacctttc
     6481 gcgcgaagct tctcgttgtc gtaaacatgc tcgccgccga acagcgcgtt aatggtttcg
     6541 tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat ttcagaaaca
     6601 tcatcgtcag cattcagacg tacgccacaa gcaccttcca tatagaggat cggggcgacg
     6661 cgcgctttca cgccttcgag acgagcgata cgggtcatca gtgccttacg tgccagcacc
     6721 agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc cagagcaata
     6781 cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat ctgctcgccg
     6841 ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggggtttt aaacgaaccg
     6901 gtgactttca ctacctgatc gtagttcagg atatccggat acatgcgctt gcttgcgcac
     6961 tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt caggccatcg
     7021 cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc gaggcctgcg
     7081 atacggttac gcaggatcga ttcctgaatc aggcgcgatt cccagctggt gcccagacca
     7141 aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt tacttcgtac
     7201 tccagcgact ggaaggcatc gtagcactct ttgatggtac gagagttagc gtagccttcg
     7261 gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct ggcggtaaca
     7321 aacggcgcca gcacttcatc gatacggtta atggtggtgc cgccataaat atggctggcg
     7381 acctgagcaa taatctgcgc agttaccgcg gttgccgtag agatcgactt cggcggttca
     7441 atctcggcgt tccccatttt aaagccctgg gtcagcatgc ctttcaggtc gatcaacatg
     7501 cagttgaaca tcgggaagaa cggcgagtaa tcgagatcgt gatagtgaat atcgccacgc
     7561 tcatgtgcct gcaccacgtc acgcggcagc aggtgctggc gtgcatagtg tttagccacg
     7621 atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt ggcgttttcg
     7681 ttgagtaacg aggcgttggt ctgctcgacc agaccacgga tctcctggtt caggcgaccg
     7741 cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg agccagttgt
     7801 ttgtatggac ccgacatcag ctggttttca actgcggtct ggatctcgtt gatatccacc
     7861 tggttgcgcc cctgcatctg ctcgttgaca accgcggcaa cagtggcgca ataatcggca
     7921 tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg ctctgattta
     7981 aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc tccatatttt
     8041 taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc gtttcgtttt
     8101 ccggtgcttt ccgcaatcgc catccgctta tccacaacgc cgcacctttt tcgtggggag
     8161 ctgtagtagc aattatagtc gattaataca acatattggg ttgggacgca ttttaaagtc
     8221 tatatatagt gctttgcatc aaggatgttt taactttttt tgatgtagct caaagtaaaa
     8281 agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat gaatttgctg
     8341 gttgaaaaat caacaaaaac aacatgctga cagacaaaac cccggaatga ccggcattat
     8401 cggggcttag ggaagattta cttctgtaac caccagacag cctcaaaagg ccgtaaattc
     8461 atggcacagg gttgtggtga ggcttcttcg tagttatgca tcacaagctg ccagttgccg
     8521 cgcatttgcc ctggctgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag
     8581 gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc
     8641 tggtaattgc cccatgtcag gatggcttct tgcttacgca gtgcgattaa cttttggtag
     8701 gtgtaaaaca cagaggagtc atcggccagc gcggcttcta cgttgatttg ttgatagtta
     8761 tcgcctaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttgctccat
     8821 tgcatgggcg tgcggctgtt gtcacgggat ttactggcga ggattgccaa taactcgtcg
     8881 gcatcacgac catcgttgcg cagctcggca aacatattga ggctctccac gtcgcgatag
     8941 tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct cttcgccctg gtagatatac
     9001 ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat
     9061 tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag
     9121 gcattccatg tcacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc
     9181 acaaagtcag gtttagccaa cgtccatttt tcaccaccgg gataatcgac cttcaggtga
     9241 tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc
     9301 tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg
     9361 cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa gcgacgcccg
     9421 tcgccatcca ggtcttcagg gaaacgcggg tctttggaga tcagattcac cacatccagg
     9481 cgcaacccgt cgaccccacg atcggcccag aattcacaga cttttttcag ctctgcgcgt
     9541 accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac
     9601 tgttcgcttt ccgtatgcca gcgccacgca ctaccgccaa attttgaacg ccagttgttc
     9661 ggtggcgttt ctggttcgcc atcgcgccag atataaaact ggcggtaagg gctttctttg
     9721 ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga
     9781 atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc gtccagcgtg
     9841 ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt aaccgttatc gacctgggga
     9901 gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg
     9961 cgttggataa cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt
    10021 ggataaatct ggtagataac gccgttttgc caccagtggg gaagattagt cattacgtta
    10081 ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt
    10141 gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc
    10201 gatagccatt gccagcgcaa atacctgcca gtagctcggt tgaatcgaga gaattcccgg
    10261 caggccccct acgccgatac cattcgccat aacgccgttc aggccgcata gcaatcctgc
    10321 cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta
    10381 cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg agatctcgcg
    10441 ttcattgtgc ttgcggctgg aaatgataat gcctatcacg gcggagccct gagcgatatt
    10501 cgacagcgca atcagcggcc acactggcgt gccacccatg ctttgaatca tctgcaaatc
    10561 aatagcaagg gtggtctggt gtacgccggt gatcaccagc ggcgcgtaca ggaagccaaa
    10621 caatgccgca ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac
    10681 gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag
    10741 gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggtacga tgcgtttaag
    10801 gcgagtttca ataacgccca gcgccagccc ggctaacagt gccgggatca cctgcgcctg
    10861 atagcccact ttggcgatgc tgaacatgcc aaagtcccac acttccggca gctgctgccc
    10921 gagcagataa gcgttcatca gctgggagga aaccagtgtc acgccaagca cgataccaag
    10981 gatcggcgtg ccgcccattt ttttcaccgc tgaccagcaa atcccgaccg gcaggtagaa
    11041 gaaaatcgct tcaccgatca accacagaaa atcgtagatc gtttgcaggg aagggtacat
    11101 ttgcgccagc gtctgaccgt tgctcatggg caaatcgccg atcacattgc gaaaaccgag
    11161 gatcaaaccg ccgctaatca acgcgggcag caacgggaag aagatctccg cgaaatgaga
    11221 gatcaactgc tcatgctatt tcatattttg ccgggcggct ttttttacct gctctttatc
    11281 aacctgcgcc tgtccggttg acgctattag tgcttgatag taatcaccca cgttggtgcc
    11341 aatcaccacc tgaaattgcc cggcattggt gaaacagcct ttcaccatgg ggagttgctc
    11401 aatttctttc ggtctggcat tggccggttg gttgaggaca aagcgcaggc gagtaataca
    11461 gtggctcacc gtcgcaatat tgccgcgccc gccgaccagt tcaatcaacc gatcgatatc
    11521 cgtttggttt attttgctca tcataaagcc ccatggcaga tgacattttt ggttggctgc
    11581 agaatatatt gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca gcgaagatca
    11641 caatttatcg ttcaggaaac gatcaggaca gggtggcggg gatgatgatt tgttgcggtt
    11701 cgctgcgccc ggttacctgt gcgatcaatt ggcaggccgc ctggcgtcca gcttcggcgt
    11761 agccaggatc tacggtgacg atctccggat gaaggaattt cattaacggc gtattaccta
    11821 cgctcgccag ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt gcgccaagag
    11881 ccagcgtgtc ggttgcgcac agtaacgcgg tagtttcagg cgtaatcact tttgctacgt
    11941 tttcatagcc ttgcttcata gcaagccctg gcagagcggc aacgggatgc agtttatgcg
    12001 ctttgcagaa cgccaggtag gcttcgtgac gtcgcttccc ggtcgtcacg tcactatgcg
    12061 gcacgccgag ataactgata ttgcgatgcc cctggtcata gagccgttgc atcagaatct
    12121 tgattgcccc ttcgtcgtca taacagaccg aagcaaagcc ttttgcgtca cgcgccagca
    12181 gaaccagcga tgactgccag tgggctaaca tttcttcggt tatgccggta aaaccgaaca
    12241 gcactacgcc gtcgatatta cgccgtttca gcacccccaa atgttcggca actaattgtg
    12301 gggaaaactg attttccatc atgattggat cgtaaccttg ttcatagaac gctggcagca
    12361 tggtttgaac ggcgagattt tctgacaacg aatccagacg ggtaacaatg atggcgacca
    12421 ctttatcgct ttgcccacgc atagcgcgcg cagagcggga aggggaaaat ccatgctgat
    12481 tcatcactgc ttcaacacgc tcgcgcgtgc gctgactcac gccgctttcg ttattcagca
    12541 cccgggaaac cgtagatttc cccacgccgc ttaagcgcgc gatgtctttg atggtcagcc
    12601 gattttgcat cctgttgtcc cataacgtgt tgtttaatta tttgagccta actttacccg
    12661 tgcattcagc aatgggtaaa gtctggttta tcgttggttt agttgccagc aggtattata
    12721 tcgccataaa tgctacgaat attattggat tctccttatt atttgcggcg cttttttcac
    12781 ttaccggagg ttatatggaa cctgatccca cgcctctccc tcgacggaga ttaaaacttt
    12841 tccggtaagc ccgtcttttc acggcgttac cggatgcgta aggccgtgac gttttaatgt
    12901 ccctgctcag ctttattacc ttcaggtaag gcttcgccac gcctgaagac atttctgtac
    12961 tgtttcagac agtgcggagg gactccttat gtttaaagaa atttttaccc ggttcattcg
    13021 ccatttacct tcccgtctgg ttcatcgcga tccattgcct ggcgcgcagc agacagtgaa
    13081 tacggcggtc ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga tgcccgaaga
    13141 agaattgtgg aaaacgttcg acacccatcc ggaaggatta aatcaggcgg aagtggaatc
    13201 tgcccgcgaa caacatggtg ataataaatt acccgcccaa caaccgtcgc cgtggtgggt
    13261 acatttatgg gtctgctatc gcaacccctt taatattttg ctcaccattc ttggtgctat
    13321 ttcttacgcc acggaagatt tatttgccgc aggtgttatc gcgctaatgg tcgctatttc
    13381 tacgttgctg aactttattc aggaagctcg ttccaccaaa gcggcagatg ccctgaaagc
    13441 gatggtcagt aataccgcga cggtactgcg agtaattaac gacaaaggcg aaaatggctg
    13501 gctggagatc ccgatagacc agctggtgcc cggcgatatt ataaaactgg cggcggggga
    13561 tatgatcccg gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg ctcaggcgtc
    13621 gttaaccggt gagtctctgc ccgtggaaaa agccgctact actcgccagc cggagcacag
    13681 caatccgctg gagtgcgaca ccctgtgttt tatgggcacc accgtggtga gcggcacggc
    13741 acaagcaatg gtgattgcta caggagccaa cacctggttt ggtcaactgg cggggcgtgt
    13801 tagtgagcag gaaagcgagc cgaatgcctt tcagcaaggg atcagccgcg ttagtatgct
    13861 gctgattcgc tttatgctgg tgatggctcc ggtggtgttg cttatcaacg gttacaccaa
    13921 aggcgactgg tgggaagcgg cactgtttgc gctttcggtc gcggtaggct taacgccgga
    13981 aatgttgccg atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc tgtcgaaaca
    14041 gaaagtgatc gtcaaacatc tggatgccat tcagaacttt ggcgcaatgg atattctgtg
    14101 cactgataaa accggcaccc tgacgcagga caaaattgtg ctggagaatc ataccgatat
    14161 ctccggtaaa accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc attaccagac
    14221 cggacttaaa aatctgcttg ataccgcggt gctcgaaggt acggatgaag agtcagcgcg
    14281 ttcgctggcc agccgttggc aaaaaattga tgagattccg tttgatttcg agcgtcgtcg
    14341 gatgtcggtg gtagtggcag aaaataccga acaccatcag ctggtttgca aaggtgcatt
    14401 gcaggaaatc ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg taccgctcga
    14461 tgacaccatg ctgcgtaaga ttaagcgggt tactgatacg ctgaatcgtc aggggctgcg
    14521 cgtggttgcg gtggcgacga aatacctgcc agcgcgtgaa ggagattacc agcgggcgga
    14581 tgaatccgac ctgatcctcg aaggatatat tgctttcctt gatccgccaa aagagacaac
    14641 cgctccggca ctgaaggcat taaaagcgag tggaattacc gtaaaaatcc ttactggcga
    14701 cagtgagtta gttgcagcga aagtgtgcca tgaagtgggg ctggatgcgg gagaggtggt
    14761 cattggtagt gatattgaaa cgctatctga cgatgaactg gcaaatctcg cacagcgcac
    14821 cacgctgttt gcccgcctga cgccgatgca taaagaacgc attgtgacct tactgaagcg
    14881 cgaagggcat gtggttggct ttatgggcga tggtattaat gatgcgcccg ccttacgcgc
    14941 tgcggatatc ggcatttctg tggacggcgc ggtagatatt gcccgtgaag cggctgatat
    15001 catcctgctg gaaaaaagcc tgatggtgct ggaagagggg gttattgagg gacgtcgcac
    15061 tttcgccaac atgctgaaat acatcaaatt gacggcgagt tctaacttcg gtaatgtgtt
    15121 cagcgtgctg gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt tacacttgct
    15181 tattcagaac ctgctgtacg atgtgtcaca ggtggcgatc ccgtttgata acgtcgacga
    15241 cgagcaaatt caaaagccgc agcgttggaa tccggcggat ctggggcgct ttatggtctt
    15301 cttcggaccg atcagctcga ttttcgatat tctgacgttt tgcttgatgt ggtgggtatt
    15361 ccatgccaac acgccggaaa cgcaaacgct gttccagtcg ggctggtttg tggtgggatt
    15421 actgtcgcaa acgctgattg tgcatatgat ccgtacccgc cgtgtgccgt ttattcagag
    15481 ctgtgcatcg tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg ggatcgcatt
    15541 gccgttttca ccgctggcca gttatctgca gctgcaggcg ctgccgttaa gctatttccc
    15601 gtggctggtt gcgattctgg cagggtatat gacgttaacc cagttggtga aagggttcta
    15661 tagccgtcgt tacggctggc agtaaagaat aaactgggca tggtggcccg gattcagatt
    15721 gcagacaaag tgagctttgt tcatgccgga tgcggcgtaa acgcccaatc cggcctacaa
    15781 attcaataaa ttgcaggaat cacgtaggcc tgataagcgt agcgcatcgg gcgattttgc
    15841 ttttgtcatc agacttaatc cgggcatgat agcccggatt tccatcaaga ttagcgacga
    15901 acagcgattg cttcgatctc aatcttcacg tctttcggca gacgggcaac ttcaacacaa
    15961 gaacgtgccg ggaaagtggc gttgtgttcg gtgaagaagg cttcgtaagt ggcgtttacg
    16021 gttgcgaagt cgttcagatc tttcacgaac acagtggttt taacgatgtc gcccactttc
    16081 aggccagcgg cttcgacgat tgcttttacg ttatccagcg actgacgtgc ctgtgcagcg
    16141 acgtctgccg gtacttcacc agttttcgga tttaccggga tctgaccgga ggtgatgatc
    16201 atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc attttccgtc
    16261 gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg gctcattata
    16321 gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt atgggaaaac
    16381 tctttttcac agtatttgca tttaagcgcg atgtcattgg cgcgttttcg cacggcaaag
    16441 ctggatgaaa ccggttcggc atggctgata cagttgctgt tcgggcagac cagcacattg
    16501 tcgatgcgct ccggcagact tgggcgcgat ttacccacca cttcatagtt gtcgatacgg
    16561 ttaaccgtgg cttgcggtgc atacaatgcc agttggtcta cttgatcttc actcaaaaag
    16621 gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcaa attcagacca
    16681 atggtgatgc gctgatctgt ttcggtcaac ttgaacagac tcaacagctt aaaaccgatc
    16741 tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg caatttatta
    16801 tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac cagtgccagt
    16861 aacgcctggc gagcgaaaat cccgttgcct gcttgctgga agtaccaggc gtgaggcgtt
    16921 ttatccacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac tttcatattg
    16981 gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac gttggcgtac
    17041 tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc tacttccgcc
    17101 atcacttctt caatagagct gtgcagactc caggcgatcc ctttttcatc gagcatatcc
    17161 agaatgtatt gcggcattgc cagcgcatcc ggcgcgataa agtaaaaacg gttgccgtcg
    17221 aacttcgcta gcgcctgggt cagggagtga acggtgcggc catatttcag gtcaccaacc
    17281 attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatggtgaa taagtccagc
    17341 aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg tacattgcca
    17401 gaaaattcgg tggccaggcg cgccgcacct tcctgcggat gacgcatcac tatcgcatcg
    17461 acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcctttttt acccagtgat
    17521 gtattgccgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat ggaagtttcg
    17581 aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac tttgttcttc
    17641 aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac cagattaagg
    17701 tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg attagccatc
    17761 ttttatctcc tgacgcctag gcaaaaaaaa gcccctcgat tgaggggctg ggaatgggtg
    17821 atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa gacaaaatgt
    17881 cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact catagccaga
    17941 agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt gaattaaaag
    18001 tcaaaatata tgcattttta ttttctatga ttctggaggg gaagtgtcgt tcggtatcaa
    18061 taattgcaga catttcctgc ttttttcttt ttcattttca cgcaatcaga aatgcatttt
    18121 attccattca tccgacttat agggcggagg gtgaaaacga acggctaaca ctattgctta
    18181 tagctcaggg atacgcgcta tcactttaat ttcaaaatca aagcctgcca gccaggtaac
    18241 acccaccgcc gtccagtttg gataaggtgg ggcgctaaat atttcatttt tcaccgtcat
    18301 gatgtcttca aattgttttt ccggatcggt atgaaagctc gtaacatcaa tgatatcgtc
    18361 aaaagtgcat cccgcagctg ccagggtcgc atgcaaatta tcaaaggcca gtctgacttg
    18421 ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc cggaaacaaa
    18481 cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca gtgaatgtcg
    18541 gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat ttacgacatg
    18601 aacagaagat ttatatttac cgggagccgc ttttaggggg acgacgttag taaacaaaac
    18661 ccagacatca tggataatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    18721 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    18781 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcaac
    18841 atatcgcgca ttggcccgtt ggcgggatta acgtcggtat caattggtcc tggctggacg
    18901 acgttgatgg tgatccctcg cggtccaaaa tcacgggcca gcccgcgcgc catgccttgc
    18961 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    19021 acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc ttcaacagag
    19081 gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    19141 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    19201 ctcttacgaa cgacatcaat aacagcgtct ctgtcagcac tatctgtgaa tactgctgtc
    19261 gctccagtct cttgtgccag acgttcagcg gcatctttcg accccgcata ggtgaatcgt
    19321 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acgactgcca
    19381 ccgaggatga gaactgtctt accggtaaaa gcgcccataa ggactccttg atttattatg
    19441 taacatgcat tacaaaactg ttttaacttt ctgtcaacag gttttgtaat ggttactaaa
    19501 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    19561 gcaaaagtgc tttttcacca aaaaggtttc gatgcagtaa gtgtctctga agttactgat
    19621 tatcttggta ttaacccccc gagcctctac gcggcttttg gcagtaaggc tgggttattt
    19681 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    19741 gatgatcgtc cggtaggcga gtgcctggtt gaggtattaa aagaagcggc gcgcagatat
    19801 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gtattcatag tcatgatcca
    19861 caagcgcgtg atattgccat tcaatattat cacgccgcag aaacgaccat ttatgactat
    19921 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    19981 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    20041 gccctggcgg gggaagcgat aaaaactctt ctcaaggagt gatgctggcc ttgatccgaa
    20101 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctgg
    20161 cggaattgat catcttttcg tataacgata gaaatgaaac attgttttaa ttaaggagtg
    20221 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    20281 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    20341 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gtacgaagct
    20401 cgccgtgcgg agtaccatgc ccgctatctt gacattcaga ttgtgttaaa aggtcaggaa
    20461 ggcatgacct tcagcacgca acctgcaggc gcgccggata ccgactggtt agctgataaa
    20521 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatcctaaa tgaaggtgat
    20581 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    20641 caggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    20701 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    20761 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    20821 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaaat gaagccttag
    20881 atacctggta tgtgggaatg aatgattttg cccgaattgc cggggggcag aatagcagaa
    20941 gcaatattct ttctcctaga gcatttttgg agtttttggc aaagatattt accctaggtt
    21001 atgtggattt tagcaaacgc tccaacgaag cgggtagaaa tatgatggct catattgagt
    21061 tctcatctta ttctaaagat actgatggca atgaaaaaat gaagttttac atgaataatc
    21121 ctgaagggga gcgagcggat ttatctaagg tgaagataga aattacactt gcctcagctt
    21181 ctactaaagg gattcgtgaa ggacatacag tcattatatt taaacaatct gatggttcga
    21241 ctaatcgtta tgaagggaag tcttttgaaa gaaaagatga tagttcatta cacctgatta
    21301 ctaacaaggt tctggcgtgt taccaacgcg aagctaacaa gaaaatagcg cggctattaa
    21361 ataatcatca gaagttaaat aatatacagg agttaaatga ttcgcaggag ttaaataatt
    21421 cacagaagtt aaataattcg caggagttaa ataactcgca ggtgagttgt aaaggttcag
    21481 ttgattctac gattacggat ttattagaaa aagcattgaa
//
LOCUS       DAJTRQ010000061        21407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000061 DAJTRQ010000000
VERSION     DAJTRQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..2007)
                     /gene="glyS"
                     /locus_tag="OO332_002342"
     CDS             complement(<1..2007)
                     /gene="glyS"
                     /locus_tag="OO332_002342"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5942795.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRL"
     gene            complement(2017..2928)
                     /gene="glyQ"
                     /locus_tag="OO332_002343"
     CDS             complement(2017..2928)
                     /gene="glyQ"
                     /locus_tag="OO332_002343"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5942796.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3023..3322)
                     /locus_tag="OO332_002344"
     CDS             complement(3023..3322)
                     /locus_tag="OO332_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5942797.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3497..4492
                     /gene="wecH"
                     /locus_tag="OO332_002345"
     CDS             3497..4492
                     /gene="wecH"
                     /locus_tag="OO332_002345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5942798.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4534..4971)
                     /locus_tag="OO332_002346"
     CDS             complement(4534..4971)
                     /locus_tag="OO332_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5942799.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5017..5358)
                     /locus_tag="OO332_002347"
     CDS             complement(5017..5358)
                     /locus_tag="OO332_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942800.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5628..6980)
                     /gene="xylB"
                     /locus_tag="OO332_002348"
     CDS             complement(5628..6980)
                     /gene="xylB"
                     /locus_tag="OO332_002348"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5942801.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7052..8374)
                     /gene="xylA"
                     /locus_tag="OO332_002349"
                     /pseudo
     CDS             complement(7052..8374)
                     /gene="xylA"
                     /locus_tag="OO332_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8740..9732
                     /gene="xylF"
                     /locus_tag="OO332_002350"
     CDS             8740..9732
                     /gene="xylF"
                     /locus_tag="OO332_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5942802.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9810..11351
                     /gene="xylG"
                     /locus_tag="OO332_002351"
     CDS             9810..11351
                     /gene="xylG"
                     /locus_tag="OO332_002351"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5942803.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11329..12510
                     /gene="xylH"
                     /locus_tag="OO332_002352"
     CDS             11329..12510
                     /gene="xylH"
                     /locus_tag="OO332_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5942804.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12588..13118
                     /locus_tag="OO332_002353"
     CDS             12588..13118
                     /locus_tag="OO332_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5942805.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13230..13766
                     /locus_tag="OO332_002354"
     CDS             13230..13766
                     /locus_tag="OO332_002354"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5942806.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGTRQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13874..14698)
                     /locus_tag="OO332_002355"
     CDS             complement(13874..14698)
                     /locus_tag="OO332_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5942807.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15018..17048
                     /gene="malS"
                     /locus_tag="OO332_002356"
                     /pseudo
     CDS             15018..17048
                     /gene="malS"
                     /locus_tag="OO332_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17226..18479
                     /gene="avtA"
                     /locus_tag="OO332_002357"
     CDS             17226..18479
                     /gene="avtA"
                     /locus_tag="OO332_002357"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5942808.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18630..19103)
                     /locus_tag="OO332_002358"
     CDS             complement(18630..19103)
                     /locus_tag="OO332_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5942809.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19205..20053)
                     /gene="yiaJ"
                     /locus_tag="OO332_002359"
     CDS             complement(19205..20053)
                     /gene="yiaJ"
                     /locus_tag="OO332_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5942810.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20254..21252
                     /gene="yiaK"
                     /locus_tag="OO332_002360"
     CDS             20254..21252
                     /gene="yiaK"
                     /locus_tag="OO332_002360"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5942811.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21264..>21407
                     /locus_tag="OO332_002361"
     CDS             21264..>21407
                     /locus_tag="OO332_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5942812.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5380 a         5461 c         5257 g         5309 t
ORIGIN      
        1 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
       61 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      121 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      181 cggctctttc agggtagagg cattcacgcg atcgctcagc acttcgtcag atttcgccag
      241 gatgttagat acacgtttgt tcgccgccgc cagtgctgca gctgcttcca gggtacggaa
      301 gtgcgatacc gctttcatgc gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      361 taccgcctgg atggtgtcaa cggtataacc ttcgtcctga taccaggcgc ggaagcgacc
      421 gagcataaag tcgataacat catcaactac gttggcatta gtcagcttat cgccatacag
      481 acgcaccgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      541 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      601 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      661 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      721 cgcgacggct acgtcttccg cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc
      781 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
      841 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttcg gcgatccagc cagccagcgc
      901 ctggatgcgg tcagtcttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
      961 caggcgcggc aggttatctt ccagacgttt tttgcggtcg gtgttgaaga agaactcggc
     1021 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1081 cgattcgatg ttggcaacga agataaagtt cggcagcagt ttgccgtcgt tcgcatacac
     1141 cgggaagtac ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1201 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1261 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1321 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1381 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1441 gtggccgcga atcacgcgat cggactgaat gcccagaatg gttgccggaa tgactttgtc
     1501 gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcacccca
     1561 gcgcatcagt ttcgggatcg gcagtttcgc cagcgaagtc gcaaccatat tcggcagcag
     1621 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1681 agtcagacgc tcagcctggt caacggtaat accacaacca cgcgcccaac cttctgccgc
     1741 tttgctcggt tttccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1801 ttcacgatct ggttgtgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     1861 agcaaaccat tgaacggtgc cgtgtgcgag gcctgcgtta tccagctccg cagtaaagtt
     1921 cgcagcaaag gactctgcca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     1981 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2041 gaagccgagg gcttcacggg aagcgtaata tgcttctgcc actgctttgg tcagggtgcg
     2101 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2161 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2221 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2281 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2341 gccgtaggtg gttttaccca gcgggccgtc gctccagact aggtcgtaaa cgctgtctac
     2401 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2461 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2521 ccacacttcc cagcccagtc cccaggcccc cagagtcggg ttttcccagt tatcttccac
     2581 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2641 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2701 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2761 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     2821 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     2881 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     2941 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3001 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3061 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3121 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3181 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3241 gtgctttctg cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg
     3301 gaaagaaagc gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt
     3361 atatcggtat agtggctgat atcttacgaa tttccttaag cctctatagg ctgtagtgat
     3421 aaaactcaga ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc
     3481 agatacagag acacagatgc agcccaaaat ttactggatt gataacctgc gagggatagc
     3541 gtgtttaatg gtggtgatga ttcacaccac tacctggtat gtgaccaatg ctcatagtgt
     3601 tagccccgtc acatgggata tcgccaatgt tctgaattct gcctctcgtg tcagcgtgcc
     3661 gctatttttc atgatttccg gctatctctt ttttggcgaa cgcagcgccc agccgcgcca
     3721 tttcttgcgt atcggcttat gtctgatttt ttatagcgca atcgcactgc tctacattgc
     3781 gctgtttacc tccatcaata tggagttagc gctgaaaaac ctgctgcaaa agccagtgtt
     3841 ttaccacttg tggtttttct tcgcgattgc ggtgatttat ctggtttcac cgctgattca
     3901 ggtgaagaac gtcggcggaa aaatgttgct ggtgctaatg gtggtgattg gcattatcgc
     3961 taacccaaac acagtgccgc agaaaattga cggttttgaa tggctgccaa ttaacttata
     4021 tatcaatggc gatacttttt actacattct gtatggcatg ttgggccgcg ctatagggat
     4081 gatggacaca cagcataaag cactgtcgtg ggtgagcgcc gcgctgtttg cgacgggggt
     4141 ttttattatc tctcgcggga cattatatga attgcagtgg cgcggaaatt ttgccgatac
     4201 ctggtatctt tactgtgggc cgatggtttt tatctgcgca atcgcgctat tgactctggt
     4261 taaaaacacg ctggatacgc gtaccattcg cggacttggc ttaatctccc gccattcatt
     4321 gggtatatac ggattccacg ccttgattat ccatgcgctg cgcacccggg gaattgagct
     4381 taaaaattgg ccaatactgg atattatttg gatcttttgc gcgacgttgg cagcgagttt
     4441 gttactttct atgctggtac aacgaatcga cagaaacaga ttagtgagtt aagtaaaagc
     4501 ccgttcacat tggactgacc gggcttacgt gaattattcg ctgtattctt cctgggtaac
     4561 ctgtgtttct tttggcgggt ttattccggc atcacgaata ttcttctgca ccgctactgc
     4621 accaaacaag cttaagaaga aagccagtcc ataaaaacct ttttcactga gtaataatgt
     4681 cgcattccac agacctacca tcagtagagc aacagagata ataaagacag tcaggcaggt
     4741 catataataa atggaagtgg tcggtatgcc ttcatacttg tcccgaacgg tcttttgata
     4801 agacgcagca gaaaacagtc ctaacaccag tacggcaaaa taataccctt tttcatttaa
     4861 ctgcatctct gcattccata gccctaacag gtaggtaacg ataccaccaa cgagagctat
     4921 ccatgacaca atactaaagg ccggtgaata ggttgatatt ttgttgtcca tcacagtatt
     4981 cctttcattc ctgaatgtgt aagagcttta tgttgctcat accgagcgaa acaaacgcat
     5041 ttgaccaaac aagcagacaa aaaaggcgat ggggtagatc accatttcat aagcattgat
     5101 aggtgcattt aaaacaccga taaacaagag accgatagtg attaacgcaa ctaactggca
     5161 gatatgggta aaaaaattat ccagttgccc gagtttttct gcgcgaagat atgcatagtt
     5221 accaaaagtt gctgtcatta acacgccaag aaaatagcct tttccactta gcaatgggca
     5281 tgagatccat agcccaacca gataaaccag cgccccgaca acaaataata gttttgccac
     5341 agtttttgag gttttcataa cgatctccat atctaccagc gatacattac gagtaaccaa
     5401 cgaaagacaa aactgaaaaa tgccattaac aaatgatttt cagaataaat tcatactaaa
     5461 tattaattaa ttagtgagat atatagatgt gaattatccc ccacccggtc aggcagggga
     5521 taacgtttac gccattaatg gcagaagttg ctgatagagg cgacggaacg tttcacgtcg
     5581 tggctgataa gcggcataac gctgcgcatc tggtagatgc gactgttcta acggtagctg
     5641 cggcaacaac tcaatgagcg atttctctgg attcgccgca atctgcgcca gccttgctgc
     5701 gcccagtgct ggccccacat cccccccgta cggtaatcga gctgctgacc gctgatatcc
     5761 gccagcatct gacgccagta ctcactacgc gccccgccac caatcaacgt aacactttgc
     5821 ggtttaatgc cgcaggcatg cacgacatcc atgccatctg ccagcgcata acccacgcct
     5881 tccagcactg ctcgcgccag ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc
     5941 cccttcgcct ggggattatt gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa
     6001 actggctcgg cactttcatc agcctgttga gctgcagcga ttaaagctgg gacattgcac
     6061 aggccggtta atttcgcggc ccaatccaga cacgatgctg cactcagcat tacagacatt
     6121 aaatgccaac gctgcggtag cgcatggcaa aagctgtgta cagcgctttc tggcttgctt
     6181 aagaaccctt cgctgacagc aaaatagacc cccgacgtcc ccagcgataa cattgcctga
     6241 ttagcatcaa ccattcccac accaactgca ccagctgcat tgtcgccacc gcctgcgaca
     6301 actggcaccg tcgccatacc ccacgctttc gcaacttcag gtaacaaagc accagtaatt
     6361 tcgctgcctt cgtataatgc gggcatctgg tcacgagata agtcgcaagc ctgcagcatg
     6421 acgtcactcc agtcacgctt tgcgacatcc agccacatgg tgccagctgc gtcagacata
     6481 tcgctggcaa actcccccgt catacgcaga cgcaagtaat ctttcggtaa taatactttg
     6541 tcgatttgac ggaatatctc cggctcatgc cgctgaaccc atagcaattt aggcgcagta
     6601 aatccgggca tcatcaggtt gccggtaatc actcgtgatt gcggaactct cgcttccagc
     6661 aaagtgcact cttgcgcaca gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt
     6721 tgttgagcat ccagtaaggt tgctccatgc atctggccgg caatacccaa tgctttaacg
     6781 tcctgcagag aatgctgatc gcccagagct ttcattgcgc gatcagttgc ctgccaccac
     6841 tgttccgggt cttgttccga ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt
     6901 tgcgaagcaa ccacctcacc ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca
     6961 agatctatcc cgatatacat atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga
     7021 taaccgggcc aacggactgc acagttagcc gttatttgtc gaacagataa tgatttacca
     7081 gattttccag ttgctcctgg cgaccactct gatgcaccgg agacaaatta tgttcctgag
     7141 catatttggc taaatctgcc agtgacattt ggcctttcag gatttgctgg cccaattcgc
     7201 tattccagcc ggaataacgc tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc
     7261 gcgctgcaat tttcagcgcc agtgccatcg tatccatcgc gccgatatga ccgtaaaaca
     7321 gatcatattt atcagtactt tgacgacgta ctttggcatc gaagttcaga ccaccggtgg
     7381 tgaaaccgcc tgctttgaga atttcataca tcaccagcgc attctcttcc acactgttcg
     7441 ggaactggtc ggtgtcccag cccagttgcg catcgccacg gttggcgtcg acagaaccga
     7501 acaggccaag cgcaatggcg gtggctattt catgatggaa agagtgacct gccagcgtcg
     7561 cgtggttagc ttcaatgttc agtttaatct ctttttccag accaaactgt ttcaggaagc
     7621 catagaccgt cgcggcatcg taatcatatt gatgtttagt cggttcttgc ggtttcggtt
     7681 cgataagcaa cgtgccctag aagccgattt tatgtttatg ctcaaccacc atctgcataa
     7741 agcggcccag ttgttcacgc tcctgacgca agtcggtatt taacagcgtt tcgtaacctt
     7801 cacgaccacc ccacaggaca tagttttcac cgcccaattt atgggttgct tccatcgctg
     7861 taacaacttg cgttgccgcc cagctgaaga cttcaggatc tgggttcgtc gccgcacccg
     7921 cgccatagcg agggtttgta aagcagttag cggttcccca cagcagcttc acgccgctct
     7981 cttcttgctt ggctgccagg acatcaacca tttgcgcaaa attattgatg tactctttta
     8041 acgacgcgcc ctcaggggaa acatccacat cgtggaagca ataaaatggc acatgtaact
     8101 tgtggaaaaa ctcaaatgcg acatctgctt tacgcttcgc caacgccagt gcctcaccag
     8161 gctgctgcca cggacgatta aacgccccca caccaaacat gtccgccccg ttccagcaga
     8221 aggtgtgcca gtagcaggcg gcaaaacgca agtgctcttc catacgctta cccaacacca
     8281 gttcgtcggg attgtagtga cggaatgcta acgggtttga ggattttgag ccttcataac
     8341 gaacgcgatc gagctggtca aaataggctt gcatattgaa ctccataatc aggtaatgcc
     8401 gcgggtgatg gatgatgtcg taatattggg cactcccttt caattgctca attatgttat
     8461 ttcacactgc tattgagata attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg
     8521 atgaaactgg gtaattcctc gaagataaaa atgcaataag tacaattgcg caacaaaagt
     8581 aagatctcgg tcataaatca agaaataaac caaaaatcgt aatcgaaaga taaaaatctg
     8641 taattgtttt cccctgttta gttgctaaaa attggttacg tttatcgcgg tgattgttac
     8701 ttattaaagc tgtcctctaa ctacagaagg ccctacacca tgaaaataaa gaacattcta
     8761 ctcacccttt gcacctcact tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa
     8821 ataggtatgg cgattgatga tctccgtctt gaacgctggc aaaaagatcg agatattttt
     8881 gtgaaaaagg cagaatctct cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa
     8941 gaaacacaaa tgtcgcagat tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt
     9001 attccgtata acggtcaggt attaagtaac gttgtaaaag aagccaaaca agaaggtatt
     9061 aaagtattag cttacgaccg tatgattaac gatgcggata tcgattttta tatttctttc
     9121 gataacgaaa aagtcggtga actgcaggca aaagccttgg tcgatattgt tccgcaaggt
     9181 aattacttcc tgatgggcgg ctcgccggta gataacaacg ccaagctgtt ccgcgccgga
     9241 caaatgaaag tgttaaaacc ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa
     9301 tgggttgatg gctggttacc ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc
     9361 aataataaca aaattgatgc tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt
     9421 caggcattaa gcgcgcaagg tttatcaggg aaagtagcaa tttccggcca ggatgcggat
     9481 ctcgcaggta ttaaacgtat tgctgccggt acgcaaacta tgacggtgta taaacctatt
     9541 acattgctgg caaatactgc cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca
     9601 aaagcagata cctcactgaa taatggcctg aaagatgtcc cctcccgcct cctgacaccg
     9661 atcgatgtga ataaaaacaa catcaaagat acggtaatta aagacggatt ccacaaagag
     9721 agagagctgt aagcgttacg ccccggcgcg gagcgggggc gtgatttctc tccatgccgc
     9781 gtgaatgaat tggcttaggt ggagtcgtta tgccttatct acttgaaatg aagaacatta
     9841 ccaaaacctt cggcagtgtg aaggcgattg ataacgtcag cttgcggttg aatgctggcg
     9901 aaatcgtctc actctgtggg gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt
     9961 gtggtattta tccccatggc tcctacgaag gcgaaattat ttttgcggga gaagagattc
    10021 aggcgagtca catccgcgat accgaacgca aaggtatcgc catcattcac caggaattgg
    10081 ccctggtgaa agaattgacc gtgctggaaa atatcttcct gggtaacgaa ataacccaca
    10141 atggcattat ggattatgac ctgatgacgc tacgctatca gaagctgctc gcacaggtca
    10201 gtttatccat ttcacctgat acccgcgttg gcgatttagg gcttgggcaa caacaactgg
    10261 ttgaaattgc caaggcactt aataaacagg tgcgcttgtt aattctcgat gaaccgacag
    10321 cctcattaac tgagcaggaa acgtcggttt tactggatat tattcgcgat ctacaacagc
    10381 acggtatcgc ctgtatttat atttcgcaca aactcaacga agtcaaagcg atttccgata
    10441 cgatttgcgt tattcgcgac ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg
    10501 aagacgatat tatcaccatg atggtcgggc gagagttaac cgcgctttac cctaatgaac
    10561 cacataccac cggagatgaa atattacgta ttgaacatct gacggcatgg catccggtca
    10621 atcgtcatat taaacgagtt aatgatgtct cgttttccct gaaacgtggc gaaatactgg
    10681 gtattgccgg actcgttggt gccggacgta ccgagaccat tcagtgcctg tttggcgtgt
    10741 ggcccggaca atgggaagga aaaatttata ttgatggcaa acaggtagat attcgtaact
    10801 gtcagcaagc catcgcccag gggattgcga tggtacccga agacagaaag cgcgacggca
    10861 tcgttccggt aatggcggtt ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg
    10921 gtggcattag ccagcttgat gacgcggcag agcaaaaatg tattctggaa tcaattcagc
    10981 aactcaaagt taaaacgtcg tcccccgacc ttgctattgg acgtttgagc ggcggcaatc
    11041 agcaaaaagc gatcctcgcc cgctgtctgt tacttaaccc gcgcatcctc attcttgatg
    11101 aacctaccag gggtattgat attggcgcga aatatgagat ctacaaatta attaaccaac
    11161 tcgtccagca gggtattgcc gttattgtca tctcttccga attacctgaa gtgctcggcc
    11221 ttagcgatcg tgtactggtg atgcatgaag ggaaactaaa agccaacctg ataaatcata
    11281 acctgactca ggagcaggtg atggaagccg cattgaggag cgaacatcat gtcgaaaagc
    11341 aatccgtctg aagtgaaatt ggccgtaccg acatccggtg gcttttctgg gctgaaatca
    11401 ctgaatttgc aggtcttcgt gatgattgca gctatcatcg caatcatgct gttctttacc
    11461 tggaccaccg atggtgccta cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc
    11521 gcgattaccg gcatcctcgc ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac
    11581 ctttccgtcg gctcaatgat ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg
    11641 ttaggctggc ctttgccact taccatcatt gtgacgttgg ttctgggact gcttctcggt
    11701 gcctggaacg gatggtgggt cgcgtaccgc aaagtccctt catttattgt caccctcgcg
    11761 ggcatgttgg catttcgcgg catactcatt ggcatcacca acggcacgac tgtatccccc
    11821 accagcgccg cgatgtcaca aattgggcaa agctatctcc ccgccagtac cggcttcatc
    11881 attggcgcgc ttggcttaat ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt
    11941 caggctttgg gtttacagtc tccggcctct accgcagtag tcggtcgcca agctttaacc
    12001 gctatcatcg tattaggcgc aatctggctg ttgaatgatt accgtggcgt tcccactcct
    12061 gttctgctgc tgacgttgct gttactcggc ggaatgttta tggcaacgcg gacggcattt
    12121 ggacgacgca tttatgccat cggcggcaat ctggaagcag cacgtctctc cgggattaac
    12181 gttgaacgca ccaaacttgc cgtgttcgcg attaacggat taatggtagc catcgccgga
    12241 ttaatcctta gttctagact tggcgctggt tcaccttctg cgggaaatat cgccgaactg
    12301 gacgcaattg cagcatgcgt gattggcggc accagcctgg ctggcggtgt gggaagcgtt
    12361 gccggagcag taatgggggc atttatcatg gcttcactgg ataacggcat gagtatgatg
    12421 gatgtaccga ccttctggca gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg
    12481 atggactccg caaccaaacg ccgttcttga ttttgataaa aattttctca aagccggtta
    12541 cgtattaccg gttttgagtt tttgcatgat tcagcaggaa aagaaccatg tttactaaac
    12601 gtcaccgcat cacattactg ttcaatgcca ataaagccta tgaccggcag gtagtagaag
    12661 gcgtagggga atatttacag gcatcacaat cagaatggga tattttcatt gaagaagatt
    12721 tccgcgcccg cattgataaa atcaaggact ggttaggaga tgtcgtcatt gccgacttcg
    12781 acgacaaaca gatcgagcaa gcgctggctg atgtcgacgt ccccattgtt ggggttggcg
    12841 gctcgtatca ccttgcagaa agttacccac ccgttcatta cattgccacc gataactatg
    12901 cgctggttga aagcgcattt ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt
    12961 atggtcttcc ggaatcaagc ggcaaacgtt gggccactga acgcgaatat gcatttcgtc
    13021 agcttgtcgc cgaagaaaag tatcgcggag tggtttatca ggggttagaa accgcgccag
    13081 agaactggca acacgcgcaa aatcggctgg cagactgact acaaacgctg ccaccgcaaa
    13141 ccgggattat tgccgttact gacgcccgag cgcggcatat tctgcaagta tgtgaacatc
    13201 tacatattcc cgtaccggaa aaattatgcg tgattggcat cgataacgaa gaactgaccc
    13261 gctatctgtc gcgtgtcgcc ctttcttcgg tcgctcaggg cacgcggcaa atgggctatc
    13321 aggcggcaaa actgttgcat cgattattag ataaagaaga aatgccgcta cagcgaattt
    13381 tggtcccacc agttcgcgtc attgaacggc gctcaacaga ttatcgctcg ctgaccgatc
    13441 ccgccgttat tcaggccatg cattacattc gtaatcacgc ctgtaaaggg attaaagtgg
    13501 atcaggtact ggatgcggtc gggatctcgc gctccaatct tgagaagcgt tttaaagaag
    13561 aggtgggtga aaccatccat gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc
    13621 tgctgatttc aaccaccttg tcgatcaatg agatatcgca aatgtgcggt tatccatcgc
    13681 tgcaatattt ctactctgtt tttaaaaaag catatgacac aacgccaaaa gagtatcgcg
    13741 atgtaaatag cgaggtcatg ttgtagcgat gatgagaatt gtcggcgtca catcagggaa
    13801 ctcagcacag ccacctgatg cgagcaaaag ccttatccga ccgacaaatc agataaggct
    13861 cgaggaaatg aaattacata tgcgccgcga ttaaccgttg gttatcctgg tacattgcga
    13921 acaggtagtt gttataactc ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg
    13981 ctgtggcagt cacttcctga tccgctttac gcagctgtgc acgcgattta cggaacgaag
    14041 agtaagccgg gtgcgtattc aggttagtga cataggcgct caccgattct ttgacagaac
    14101 taaactgtga gtaccctttc actttacccg gcgcattggt acaacgtcct ttcatgcatt
    14161 tcatgccgaa cagattgtta ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg
    14221 ctgcagccat cgtcgccacc atactggtag ggataatgtc tacgcgttca agcaaggtat
    14281 tccacgggat ttttcgcgta ttaccagacc acttcacctt gtagcgtttg gcgatgtctt
    14341 tcagacgcgc acgctcagca ggtgaccatt ggccctgata ctgttttgaa atcagccagt
    14401 tacgttctgc agtaatggct gcattttggc tggtaatgta aggcattacg gtccggagaa
    14461 acgccttttt ccttggtgtt ccggaagggt attttcgcaa atcaggaagt gaactactct
    14521 ttgcactatt gcgagaatac tcttgtttac tgcttacctg tttattacta gctttagtta
    14581 agtgggactt ttgactcgct gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca
    14641 ccagagtgag taacataaga atcatcgccc catatcgtcg tatgggagtc aaaatcatca
    14701 ggtctcctgg ccggatttaa tcattccaac accttatatt tttcacaaat ttgagagttg
    14761 aatctcaaat catatcaaaa atagctgtca agagcacccc aaggaatagt ccaaatctga
    14821 aactatgtca cgagttaacg attcagattg gcgctaaatc gcagaaaatg tgggggttat
    14881 cgcaaaattc agccgttttt tgcgcgcgat cgctcaccct tgcttctcat cctgtggact
    14941 taccgctcag ggatgagttt tgtctggctt atcgctggca aactgtctga aatcgcagca
    15001 ataaggactc atccaccatg aaactcgccg cctgttttct gacactcctt cctggcttcg
    15061 ccgttgccgc cagctggact tctccggggt tccctgcctt tagcgaacag ggaacgggaa
    15121 catttgtcag ccacgcgcag ttgcccaaag gtacgcgtcc actcacgcta aattttgacc
    15181 aacagtgctg gcagcctgca gatgcgataa aactcaatca gatgctttcc ctgcaacctt
    15241 gtagcaacac gccgcctcaa tggcgattgt tcagggacgg caaatatacg ctgcaaatag
    15301 acacccgctc cggtacgcca acattgatga tttccatcca gaacgccgcc gaaccggtag
    15361 caaacctggt ccgtgaatgc ccgaaatggg atggattacc gctcacgctg gatgtcagcg
    15421 ccactttccc ggaaggagcc gccgtacggg attattacag ccaacaaatt gcgatagtga
    15481 agaacggtca aataacgtta caacccgctg ctaccagcaa cggtttactc ctgctggaac
    15541 gggcagaaac tgacacatcc gcccctttcg actggcataa cgccacggtt tactttgtgc
    15601 tgacagatcg tttcgaaaac ggcgatccca gtaatgacca gagttacgga cgtcataaag
    15661 acggtatggc ggaaattggc acttttcacg gcggcgattt acgcggcctg accaacaaac
    15721 tggattacct ccagcagttg ggcgttaatg ctttatggat aagcgcccca tttgagcaaa
    15781 ttcacggctg ggtcggcggc ggtacaaaag gcgatttccc gcattatgcc taccacggtt
    15841 attacacaca ggactggacg aatcttgatg ccaatatggg caacgaagcc gatctacgga
    15901 cgctggttga tagcgcacat cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc
    15961 acaccagcta tgccacgctg gcggatatgc aggagtatca gtttggcgcg ttatatcttt
    16021 ctggtgacga agtgaaaaaa acgctgggtg aacgctggag cgactggaaa cctgccgccg
    16081 ggcaaacctg gcatagcttt aacgattaca ttaatttcag cgacaaaaca ggctgggata
    16141 aatggtgggg aaaaaactgg atcagaacgg atatcggcga ttacgacaat cctggattcg
    16201 acgatctcac tatgtcgctg gcctttttgc cggatatcaa aaccgaatca actaccgctt
    16261 ctggtctgcc ggtgttctat aaaaacaaaa cggatactca cgctaaagtc atcgaaggct
    16321 ttacacctcg cgattactta acccactggt taagtcagtg ggtccgcgac tatgggattg
    16381 atggttttcg ggtcgatacc gccaaacatg ttgagttgcc cgcctggcag caactgaaaa
    16441 ccgaagccag ctccgcgctt cgcgaatgga aaaaaactaa ccccgacaaa gcattagatg
    16501 acaaaccttt ctggatgacc ggtgaagcct agggccacgg cgtgatgcaa agtgactact
    16561 atcgccacgg cttcgatgcg atgatcaatt tcgattatca ggagcaggcg gcgaaagcag
    16621 tcgattgtct ggcgcagatg gatacgacct ggcagcaaat ggcggagaaa ttgcagggtt
    16681 tcaacgtgtt gagctacctc tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca
    16741 aagcagcaga gttattacta ttagcgccag gcgcggtaca aatcttttat ggcgatgaat
    16801 cctcgcgtcc gttcggtcct acaggttctg atccgctgca aggtacacgt tcggatatga
    16861 actggcagga tgttagcggt aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc
    16921 agttccgcgc ccgccatccc gcaattggcg cgggcaaaca aacgacactt tcgctgaagc
    16981 agggctacgg ctttgttcgt gagcatggcg acgataaagt gctggtcatc tgggctgggc
    17041 aacagtgact tttccggctt cccgttcgtc agtacctcgg gaagccgcca accaggataa
    17101 aatgtcagcc ctaatcagcg ttgcgggata aagcaccgct cactcttcaa cagaccgatt
    17161 tgcaccccag caaatgtagc gttattgtta ccttccttgc tacagagttc gacagatatc
    17221 ccgctatgac attctccctt tttggtgaca aatttacccg ccactccggc attacgctgc
    17281 tgatggaaga tctgaacgac ggtttacgca cgcctggcgc gattatgctc ggcggcggta
    17341 atccggcgca gatcccggaa atgcaggact acttccagac gctactgacc gacatgctgg
    17401 aaagtggcaa agcgactgat gcactgtgta actacgacgg tccacagggg gaaacggagc
    17461 tactcacact gcttgccgga atgctgcgcg agaagttggg ttgggatatc gaaccacaga
    17521 atattgcact aacaaacggc agccagagcg cgtttttcta cttatttaac ctgtttgccg
    17581 gacgccgtgc cgatggtcgg gtcaaaaaag tgctgttccc gctggcaccg gaatacattg
    17641 gctacgctga cgccggactg gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac
    17701 tgctgccgga aggccagttt aaataccacg tcgattttga gcatctgcat attggcgaag
    17761 aaaccgggat gatttgcgtc tcccggccga cgaatccaac aggcaatgtg attactgacg
    17821 aagagttgct gaagcttgac gcgctggcga atcaacacgg cattccgctg gtgattgata
    17881 acgcttatgg cgtcccgttc ccgggtatca tcttcagtga agcgcgcccg ctatggaatc
    17941 cgaatatcgt actgtgcatg agtctttcca agctggggct gcctggctcc cgctgcggca
    18001 ttatcatcgc caatgaaaaa atcatcactg ccatcaccaa tatgaacggc attatcagcc
    18061 tggcacctgg cggtattggt ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc
    18121 tgcgcctgtc tgaaacagtc atcaaaccgt tttactacca gcgtgttcag gaaactatcg
    18181 ccatcattcg ccgctattta ccggaaaatc gctgcctgat tcataaaccg gaaggagcca
    18241 ttttcctctg gctatggttt aaggatttgc ccattacgac cgagcagctc tatcagcgcc
    18301 tgaaagcacg cggcgtgctg atggtgccgg ggcacaactt cttcccggga ctggataaac
    18361 cgtggccgca tacgcatcaa tgtatgcgca tgaactacgt accagagccg gagaaaattg
    18421 aggcgggggt gaagattctg gcggaagaga tagaaagagc ttgggcagaa agtcactaaa
    18481 aattgcctga tgcgctacgc ttatcaggcc tacatgattt ctgcaatata ttgaatttgg
    18541 aagaatttgt aggccggata aggcgtttac gccgcatctg gcataaacaa agcgcactat
    18601 tccggcgttg agaaacgccg gaaaacgttt caaacaggct gctgctgtag ccgatgccgc
    18661 tgcactttct cgacatccat gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc
    18721 ggccccgtct cccgatgcca gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa
    18781 gaaacgacct ccatcgcacc aaacgggcaa gccagcatac agcttttaca gccaatgcaa
    18841 cgtgtttgtt caacgaaaat atgcccatgt tcgcggctta tcgcgtcaac agggcagaca
    18901 ttcgcgcacg gtgcatcttc acactgatga caggctactg gcgtggtcca gctgtggtct
    18961 ttaatgacac gaatacggga aataaactcg tctggtgaca acgcagcgca atcctgattc
    19021 tcctgatgcg acactgcgca agccacttca caggtacggc aaccgataca tttcgtcgca
    19081 tccgcaataa taaaccggtt catgatcatt cctttgcact aaaacagcag agtgccagag
    19141 agcgcgtccc tactgctttg atccaacgaa ggaaatgtta aagttttggc acttgtccaa
    19201 agcgttatgt gagcgcgccc aggtcatccc tgacagtaaa tcccagttca ttagaaatag
    19261 cctgcgcggt ttcacgcagt ggtttcagga gatttttctc tcccacctgt ttcagacgtg
    19321 atgtcgaaag cgaaatcgac acggcgtacg gcacccgccc atgaatatca aacaccggaa
    19381 cagcaataca ggagacgccg agttcgtttt cttctctgtc catcgccgct ccgctttcac
    19441 gaatgtgcgc cagttcgtcg aacatcgcgg gcagctcggt aatggtattg tgggttaacg
    19501 gctggatctc atgctgatgg ttttcccagt atgacttcac gtagtccggg tgaccaaacg
    19561 ccatgtagat cttgcccatt gcggaacagt agagcggcat atgctggcca atataggcac
    19621 gggttcgcag catcccggtt gtgggttcca gcttataaat caaaatagcg tgatcgtctt
    19681 cgcggctgga gaagttaatg gtttcaccag tggcgatgtt cagtgcctca agatgcggag
    19741 cggcgatatg aatgatattc agcgaagaca gcgccttctg cccgacggca ataaatttgg
    19801 tagtcaggcg gtaactccct gcggcgggcg cggtggtcac atagccgcag gactgtaatc
    19861 cctgcaataa gcgatggacg gtactcttat ttaaaccagc cagctccgaa agatgcgcca
    19921 acggacaacc gtttggatag ttgctcaaaa tctcaatcag catcaacccg cgaaacagac
    19981 tctggcttcc ggctggacgc tctttttcct gcgccatctc gttctctttt tttcccatca
    20041 cttctttccc cattttgtcg cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc
    20101 tcgacagata atttataacc acttgatttt tatgtctttt gaaattcatc aatcagattg
    20161 ctttgttaaa aagtgatcga tatatttgaa atctagtttc gcatattgaa attttaagac
    20221 aaaaaagcga tcaaaaaaac aaggaagcct aggatgaaag tgacatttga gcagttaaaa
    20281 gcagccttta atcgggtctt aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt
    20341 gcagagatgt tcgcccgcac caccgaatcc ggcgtttatt ctcacggcgt taatcgtttc
    20401 cctcgtttca ttcaacaact ggaaaacggc gatatcattc ctgatgccca acccaaacgt
    20461 ataaccagcc tcggcgcaat tgaacagtgg gatgcccagc gttcgatcgg caacctgacg
    20521 gcgaaaaaga tgatggatcg cgccattgaa ctggctgccg atcacggtat tggtctggtg
    20581 gcacttcgta atgccaacca ctggatgcgc ggcggcagct acggctggca ggcggcggaa
    20641 aaaggctata ttggcatttg ctggaccaac tccatcgccg taatgccgcc gtggggcgca
    20701 aaagagtgtc gcatcggcac taacccgctg atcgtcgcca ttccttccac gccgatcacc
    20761 atggtcgata tgtcgatgtc gatgttctct tacggcatgt tagaagttaa ccgcctggca
    20821 ggccgtcagc tcccggtcga tggtggcttt gatgatgagg gcaatttgac caaagaacct
    20881 ggcgttatcg agaagaatcg ccgcattttg ccgatgggct actggaaagg ttctggcatg
    20941 tcgattgtgc tggatatgat cgctactctc ctttccgacg gcgcatcggt tgccgaagtc
    21001 acccaggaca acagcgacga atacggcatt tcacaaattt ttattgccat tgaagtggac
    21061 aagcttatcg acggtcccac ccgcgatgcc aagctgcaac gtatcatgga ttacgttact
    21121 accgctgaac gcgctgacga aaaccaggcc atccgcttac ccggccacga atttactacc
    21181 ctgctggccg aaaaccgccg taacggcatc actgttgatg acagcgtgtg ggccaaaatc
    21241 caggcgttat gaggagataa gtcatgattt ttggacatat cgcgcagccc aatccgtgcc
    21301 gtttgcccgc agccattgaa aaggcgctcg attttctgcg cgctaccaac ttcaacgtcc
    21361 tggagccggg cgttgtcgaa atcgacggca agaatattta tgcgcaa
//
LOCUS       DAJTRQ010000062        21241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000062 DAJTRQ010000000
VERSION     DAJTRQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(34..1014)
                     /locus_tag="OO332_002362"
     CDS             complement(34..1014)
                     /locus_tag="OO332_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5942813.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1022..1732)
                     /locus_tag="OO332_002363"
     CDS             complement(1022..1732)
                     /locus_tag="OO332_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5942814.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1948..3771)
                     /gene="typA"
                     /locus_tag="OO332_002364"
     CDS             complement(1948..3771)
                     /gene="typA"
                     /locus_tag="OO332_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5942815.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4144..5553
                     /gene="glnA"
                     /locus_tag="OO332_002365"
     CDS             4144..5553
                     /gene="glnA"
                     /locus_tag="OO332_002365"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5942816.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5726..6775
                     /gene="glnL"
                     /locus_tag="OO332_002366"
     CDS             5726..6775
                     /gene="glnL"
                     /locus_tag="OO332_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5942817.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6787..8196
                     /gene="glnG"
                     /locus_tag="OO332_002367"
     CDS             6787..8196
                     /gene="glnG"
                     /locus_tag="OO332_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5942818.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8308..8418
                     /locus_tag="OO332_002368"
     CDS             8308..8418
                     /locus_tag="OO332_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5942819.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8647..10020)
                     /gene="hemN"
                     /locus_tag="OO332_002369"
     CDS             complement(8647..10020)
                     /gene="hemN"
                     /locus_tag="OO332_002369"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5942820.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10209..10718)
                     /gene="yihI"
                     /locus_tag="OO332_002370"
     CDS             complement(10209..10718)
                     /gene="yihI"
                     /locus_tag="OO332_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5942821.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11300..11932
                     /locus_tag="OO332_002371"
     CDS             11300..11932
                     /locus_tag="OO332_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5942822.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12314..15100)
                     /gene="polA"
                     /locus_tag="OO332_002372"
     CDS             complement(12314..15100)
                     /gene="polA"
                     /locus_tag="OO332_002372"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5942823.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15464..16396
                     /locus_tag="OO332_002373"
     CDS             15464..16396
                     /locus_tag="OO332_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5942824.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16437..17867)
                     /locus_tag="OO332_002374"
                     /pseudo
     CDS             complement(16437..17867)
                     /locus_tag="OO332_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18022..18648)
                     /gene="dsbA"
                     /locus_tag="OO332_002375"
     CDS             complement(18022..18648)
                     /gene="dsbA"
                     /locus_tag="OO332_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5942825.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18665..19651)
                     /gene="srkA"
                     /locus_tag="OO332_002376"
     CDS             complement(18665..19651)
                     /gene="srkA"
                     /locus_tag="OO332_002376"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5942826.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19728..19997)
                     /locus_tag="OO332_002377"
     CDS             complement(19728..19997)
                     /locus_tag="OO332_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5942827.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20067..20651
                     /gene="mobA"
                     /locus_tag="OO332_002378"
     CDS             20067..20651
                     /gene="mobA"
                     /locus_tag="OO332_002378"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5942828.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20633..21160
                     /gene="mobB"
                     /locus_tag="OO332_002379"
     CDS             20633..21160
                     /gene="mobB"
                     /locus_tag="OO332_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5942829.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5121 g         5596 t
ORIGIN      
        1 tggttttatt aactgcgtat gagttaaaac ttattagtgg ttcagaagat ggtttgcctc
       61 ttgtttgatt tgttgtagta cgttacgcac atgatttatc tgattaattg catcttcttt
      121 ttctttacgt aaccgcgcat ccagtaaatg attttctggt aggggtgttt cactcatctg
      181 gcgataaggg atccacggat tatcatcttc gtcttcaaag cggaacgccg gcgcatagta
      241 atcaacctct tcttccaggc catatgccgt cacctgcggc tcatcatcac caaggcagat
      301 aaggtgcgtt aataacgctt tgaatggcat atcaccctga cctgaaatac aggctttatg
      361 acccaggcca ccctgttctt taacgatcaa ggcatcttta atatggacct gggtaatatg
      421 cggtgccatc gtttttaagg cgtcgatggg atgctcattt gcattgatca tgttagcaaa
      481 atcaaacagt aaggaaagag actccatctc actttctttg accagcgaca ccagctcatg
      541 actttttaaa tcttcatgct gctcaatcgt aaaagtcagg ccgctgtcct gatacgtttc
      601 ccgtacatag gcaatgtcgt tagcgataat cgataatacg tcgcgcaaat taccttcata
      661 acgtggataa aaacgtacgg acgttgcccc agttttcaac gcgatggcga cggcttcgtc
      721 aatagatgcc ttatctgaag cgctggtttc aatatgaata tcaaggttca gacggcgggc
      781 tttatcccca aaggcagaga gttctttatc gtccatatta ccaagagaaa aacgctcgcc
      841 atccagaaca tggattttga cgccacgtag attattttca cttgcgatat caagtaaatc
      901 agcaggcaac actctttcca gccgcatatt taaatgaaag gcataagcat gtaaataaag
      961 aggaagagtg tcgacacgtt gtagaatctt tcttgcgtta tttattgtca ccataaaaca
     1021 cctacagatt aatgatgagg tctacggcct caaatcgcca atattcaata tctatctgga
     1081 caatattgcc ttgctggtcg gcgcgatgct tttcaattaa gatcgctggc atacccgcag
     1141 aaccgccaag atacttgctg atatcgcccg gcattctgac gggtttgaaa atcaattttt
     1201 ttactaccgt ctctttttgg tagcactttt cccagactgc agaaaatgag tggttttcaa
     1261 gttgttcaat aaaacccgga gcaacttcag gattaatata tgtttcgtga tagaaaactt
     1321 tatgtccttc cagcgccccc cagccggtga tgcgatagag ttcagttgat ggcgtcactg
     1381 caatcagtgg cgcgagcgtc tcgggaatat cgctggtacg gtttttctcg gtaaaccccc
     1441 aagaaggctc tcgcccttgc tcaattgcgg cccgctgaaa gctcgccgac agctccggac
     1501 tgtaattaaa tcgcggctgg gtcacgaacc accccttacg atccttacgg aagattttgg
     1561 attctgcctc gaggttcaac aacgcctggc gcagcgtcat acgtttaatg cccagcagtt
     1621 ctcccagctc tctttctgaa gggagttttc cacccggcgt ggtgatgcca tctttcaacc
     1681 accgatccag tttctctttt gcattttcta cggtggattg attctccgcc attgtttaat
     1741 ccttttggtt taaaccagat gaagcatctt acgcttacgc caaaaaagaa agcaattaac
     1801 ggtgtatgac tttgcagata attgtgacaa agctctctat tgacgtaatc acagacttat
     1861 aagggagata ctcgcattgg tgtgcgctca cgacaggtaa aaaaaacctg ccatcgctgg
     1921 caggtttttt atgactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg
     1981 acgatcgttt tccgtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac
     2041 cagttcgtca tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac
     2101 cagaacaaca gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag
     2161 gcagtttaca gtcaggtcgt tagagcggct atgaataccg ataatctgac cttcgtaaac
     2221 ttctgcaccg tgaccgagga acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc
     2281 gacagcttta ccctgaccgt tagagatcag tacaccgttc tgacgctgac ccacttcgcc
     2341 cggacgtacg tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat
     2401 ggtcatgaac tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg
     2461 tacgcggcct ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc
     2521 ctgcatcaca gaaccctgat gctgttcttc aacgtccagc gtcacgtttt catacggctc
     2581 ttgtttacga ccgtcgattt cacggaagat tactttcgga cgggataccg ccagttcgaa
     2641 accttcacga cgcatgtttt cgataagaac agagaggtgc agttcaccac gaccagaaac
     2701 gcggaacgca tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc
     2761 tttgttcaga cgatcgagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa
     2821 cggcgaggtg ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg
     2881 cagcgcttca acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc
     2941 cgtgatcgcc acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag
     3001 gtggcccagc actttaccga ctttcgcgtt acgggttttg ccttcgctat cgatgatagt
     3061 aacctgctgg ttcggcttca ctttaccacg cttgatgcgg ccaatgccga taacgccaac
     3121 atagctgttg taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc
     3181 cggcgcagga acgtggtcaa caatcgcctg atacagcggg gtcatgtctt ccgccatatc
     3241 ttcgtggtcc agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag
     3301 ctgctcgtcg gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc
     3361 aggacgcgcg ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc
     3421 aaacgctttt ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac
     3481 cagcagcact gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg
     3541 ccccggggta tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc
     3601 gaggatggta atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt
     3661 ttcggcacga gagtcgaacg taccggattg ttggagcagc ttgtctacca aggtggtttt
     3721 accatggtct acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc
     3781 tcaggcatta gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca
     3841 caaacatcct ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata
     3901 aaaagggtta tccaaaggtc attgcaccaa tatggtgctt aatgtttcca ttgaagcact
     3961 atattggtgc aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac
     4021 gccttttagg ggcaatttaa aagttggcac agatttcgct ttatcttttt tacggcgaca
     4081 cggccaaaat aattgcagat ttcgttacca cgacgacaat gaccaatcca ggagagttaa
     4141 agtatgtccg ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat
     4201 ttgcgcttca ccgataccaa aggtaaagaa caacacgtca ctatccctgc tcatcaggtg
     4261 aatgctgaat tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa
     4321 ggcattaacg aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg
     4381 ttcttcgccg actccaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa
     4441 ggctatgacc gtgacccgcg ctctattgcg aagcgcgccg aagattacct gcgttccact
     4501 ggcattgccg acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc
     4561 cgtttcggat catctatctc cggttcccac gttgctatcg acgatatcga aggcgcatgg
     4621 aactcctcca cccaatacga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt
     4681 tacttcccgg ttccgccggt agactctgct caggatattc gttctgaaat gtgtctggtg
     4741 atggaacaga tgggtctggt ggttgaagcc catcaccacg aagtagcgac tgctggtcag
     4801 aacgaagtgg ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac
     4861 aaatatgtcg tgcataacgt agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa
     4921 ccgatgttcg gtgataacgg ctccggtatg cactgccaca tgtctctgtc taaaaacggc
     4981 gttaacctgt tcgcaggcga caaatacgca ggtctgtctg agcaggcgct gtactacatt
     5041 ggcggcgtaa tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct
     5101 tataagcgtc tggtcccggg ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac
     5161 cgttctgcgt ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta
     5221 cgtttcccgg atccggcagc taacccgtac ctgtgctttg ctgccctgct gatggccggt
     5281 cttgatggta tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac
     5341 ctgccgccag aagaagcgaa agagatccca caggttgcag gctctctgga agaagcactg
     5401 aacgaactgg atctggaccg cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg
     5461 atcgatgcgt acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat
     5521 ccggtagagt ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcac
     5581 aacgccggga tttagttgcc gtggaaactt tcagcccatc tctgcatggg cttttttctc
     5641 cgtcaattct ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc actaaaatgg
     5701 tgcaacctgt tcaggagact gctttatggc aacaggcacg cagcccgatg ctgggcagat
     5761 cctcaactcg ctgattaaca gtattttgtt aattgatgac aatctggcga tccattacgc
     5821 caaccccgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg gtacgccgtt
     5881 accggaactg ttgagctact tctcattaaa tatcgagctg atgcaagaaa gtctggaggc
     5941 ggggcaaggc tttaccgata acgaagtgac gctggtcatc gatggtcgct cgcatatcct
     6001 ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg caccgatgga
     6061 taaccagcgc cgcttaagcc aggaacaact acaacatgct caacaagtag ctgcccgtga
     6121 tttagtgcgc ggcctggcac atgagattaa aaatccgctt ggcggtttac gtggcgcggc
     6181 gcagttactc agcaaagcgt tacctgaccc atcactactc gaatatacca aagtgattat
     6241 cgaacaggcg gaccggctgc gaaatttggt cgaccgtctg ttggggccgc agctgcccgg
     6301 tacgcgcgtt accgaaagta ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat
     6361 ggaactgccg gacaacgtgc ggttgattcg tgattacgat cccagcctgc cggaactggc
     6421 gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc
     6481 gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc aactgacctt
     6541 acacggcgag cgctaccggc tggcggcgcg gattgatgtg gaagataacg ggccgggcat
     6601 tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg aaggtggcac
     6661 cgggcttggc ttatccatcg cccgtaattt gattgatcag cattcaggca aaattgaatt
     6721 taccagttgg ccaggtcata ccgagttctc ggtttacctg cctatcagga aataaaggtg
     6781 acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat ccgttgggtg
     6841 cttgaacgtg cgctcgctgg agcgggttta acctgtacga catttgagaa cggcgcggaa
     6901 gtactggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat ccgtatgccg
     6961 ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat gcttccggtc
     7021 atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca acaaggggcg
     7081 tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt tgagcgcgct
     7141 atcagtcatt accaggaaca gcagcagccg cgtaatattc agcttaacgg cccaacgacc
     7201 gatatcatcg gcgaagcgca agccatgcag gacgtgttcc gtattatcgg tcggctttcg
     7261 cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga actggtcgct
     7321 catgccctgc atcgccacag tccgcgcacc aaagcgccgt ttatcgcgct gaatatggca
     7381 gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact
     7441 ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggcac gttatttctc
     7501 gacgaaattg gagatatgcc gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac
     7561 ggtcagtttt atcgcgttgg cggctatgcg ccggtgaaag tggatgtgcg gattatcgct
     7621 gccactcacc agaatcttga acagcgggtg caggaaggta agttccgtga ggatctgttc
     7681 caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt
     7741 ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag
     7801 ttgctgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg caacgtgcgc
     7861 cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga agtgttgatt
     7921 caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc gcaaatgcaa
     7981 ccggacagtt gggcgacact tttagcgcag tgggcagaca gagcgctgcg ttccggtcat
     8041 caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac gaccgcgttg
     8101 cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg ccgcaacact
     8161 ctgacgcgta agttaaaaga gctggggatg gagtgattca cggcttgtgt gtaaagattg
     8221 attattgagc gcaaattgct ggtattttgc gctttactgt tccgataagt tcagtatgat
     8281 cttgcccgga aaacggggag agtcattatg ctggaatcaa taattaatct ggtatcgagt
     8341 ggcgcagttg acagccacac accgcaaact gccgttgctg ccgtgctgtg tgccgcgatg
     8401 attgggctgt ttagctgaag ctcgttgttt atgccggatg cggagaaagt cgtaggcctg
     8461 ataagacgcg accagcgtcg catcaggcat catgctcaga tgcctgatgc ggcgtaaacg
     8521 ccttatccgg cctacaaaag caataatttc aacaggtttt gttgcccagc gcatcaggca
     8581 atcttgttct cagcacattg atggcgcttt cgttttctac tttgtaaacg aagcgccatt
     8641 cactctttaa atcacccgtg agaactgctg catccgcgct ttctggcgca gataggtatc
     8701 aaagcacatg caaatgttgc ggatcagcaa gcgacctttc gccgtcacct gtattccctt
     8761 ctcatccaca tccaccagcc catcttttgc taacggggcg agcagcttga gatcttccgc
     8821 aaagtaatca gcgaagtgca aatcccactg tttctcaaga ggggcgtaat ccagacggaa
     8881 gttgcagatg agcgacttaa tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat
     8941 accacgccac agcgcattgc cttgttcatc cacttgctga tagtactgct tcaactcttt
     9001 ctggttctgc gcgtagcagt cgccaatcat gctgatggcg gaaacgccca tccccagcag
     9061 atcggtatcg ccctgagtgg tgtagccctg gaagttacga tgcagcacgc cttcacgctg
     9121 ggccaccgcc agctcgtcat caggacgggc aaagtgatcc ataccgataa actgatagcc
     9181 cgattgcgtc aggaaggcga tggtttcctg caggatatcg agtttttgct gcggactcgg
     9241 caggtcagca tctttgattt tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt
     9301 aaaaacgctc agacgatcgg ggttcagctc cgccacacgt ttcagggtaa aggcgaaact
     9361 ctccggcgtc tgtttcggca ggccgtaaat caggtcgatg ttggtggagg taaaaccaat
     9421 ctcacgcgca tggttaagca gtgcaaagat gaactcttca tcctgctcgc ggttaaccag
     9481 acgttgcact tctttgttga agtcctgcac gcccatgctc aggcgattaa agccttcggc
     9541 gcgtaaatga tcgagtacat ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc
     9601 cgcatcggta ttgaaatgga agttttcgcg cagcagcttc atcagacggc tgatttgcgc
     9661 tttattcaga tacgtcggcg ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc
     9721 gaacagcggt gcacgatgga cgatttcttg ctccagcgcg tccagatact gatcggcctt
     9781 gtgctgctgg cgagtaacaa tcttattgca accgcagaag taacaaagct tatggcagaa
     9841 cgggatatgt acgtagagag ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa
     9901 cgcctgttcg ccgaagtctt ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg
     9961 cccggaatag ttatatttct ggatcagggc cagatcccag tcgatttgct gtacagacat
    10021 gctcactcct tccgatggcg tctctggcgg ctacggcgta ccggcggatg cccgtttcgg
    10081 gtcatcagcc ggttgcgcag ccacttttgt cgccgcgaca accgctgtag tttaacgaat
    10141 aaccacacca gataacatat aaccggaagg gttatcagca gaacgggaag gcccacggcg
    10201 gcaatccgtt agttgccccg cagcagacgc atcatgtctt cttgcttctc gtcttcttcc
    10261 tcttcttcgt catcgtcata agagaggccg agtttctgca tcaactcatc aatacgatcc
    10321 agtttagcat ccacccagga ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt
    10381 tccagcagcg catccagacg ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa
    10441 agcataggtt tctcactctt cggtttgtgc tgtttggtga ctttttcagt cactcccaat
    10501 ggaatagggg ttttactgcc aatacgtgga tcttttggtg cgttctggcc tttgctgcct
    10561 gacgtggtgt ttccgcccgc tgcgcggctg cccggcgcat gaccacgacg ttttttctga
    10621 cgcttacggt cacgagcttc ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca
    10681 tgacctttgc tgcgtgagtt tgaagatgat ggtttcataa tggttcgtct tttagccgtt
    10741 ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca
    10801 gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca
    10861 ggccgccaac acaccctgtt tttccattag ccggaacaat ccgtgttgat tccatttccg
    10921 tttaattacg tctccggacg tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc
    10981 tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt
    11041 tacgcctttg ctttaaacaa acaatcaacc gccatcagta tttgcacatt ttcagagttt
    11101 tatataaaag caattcactg atttatatag cttatttgac atgcatcgca gaattttctc
    11161 tgctatttcg cataaagact atcggcaatg tctcacaaag cgtatggctt ttacttacaa
    11221 cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgcca caaagctttg
    11281 actgacggag acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag
    11341 tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg
    11401 ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg
    11461 tacctcaaaa accccagggc gcacccagct tatcaacctg tttgaagtgg ctgacggcaa
    11521 gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa
    11581 atggcagcgt gcgctcggcg aatacctcga aaaacgtcag agcctgcaag gtctggtggt
    11641 gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt
    11701 agacagcaat atcgccgttc tggtgctgct gaccaaagcg gacaaactgg caagcggcgc
    11761 acgtaaagcg ctattgaata tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca
    11821 ggttgaaacg ttttcttcgt tgaagaaaca aggcgtagac aagctgcggc agaaactgga
    11881 tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg
    11941 ccttaatgct tgtgccggat gtggcgtatc cggcccgtaa attcaataga ttacaattct
    12001 accgttggcc tgataaacat agcgcatcag gcattacgga tcttttcttt cgcccaataa
    12061 aaaacgcccc agtcattact gactggggcg gctaaaatat tcagccaaat ccgattacgt
    12121 gaagtaaaag gtctgaaaga tagaacatct tacctctgta ccctacgcga ctaactttac
    12181 tcttttttgt tcagttcaga aagcactttt tgtaattttt tttcagttgt tgcataggaa
    12241 tttctaatag ccatcacaaa acgtgacagc ttatgttgct tacttacgaa aaaagggcat
    12301 gttcaggcga atcttagtgc gcctgatccc agttttcgcc actccccact tccaccagca
    12361 acggcacatc cagacgggta cagttttcca tcagttgatg aatctgcttc gcgacggcat
    12421 caacatcatc tttatgaact tcaaatacca gttcatcgtg tacctgcatg atcatacgta
    12481 cacgcggttg ctcagcctgt aaccacgcat caacggcaat catcgcccgt ttgataatgt
    12541 cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag
    12601 caccattgct ggatttgata tccggcagat acagacggcg tccgtccagc gtttcaacgt
    12661 agcccagctc tttcgcctga gcacgggtgc gttccatata ctccagcacg ccagggtagc
    12721 gttcgaagta aaggtccatg tacttctgcg cttctttacg tggaatgttc aattgccgcg
    12781 ccagaccgaa agcactcatg ccataaatca gaccaaagtt gatcgctttc gcgctacggc
    12841 gttgctcgct ggtgacggtt tccagtggca aaccaaacac ttctgccgcc gttgcccggt
    12901 ggatatcttt tccttccgcg aatgcggtca gcaagccttt atcacgcgag agatgcgcca
    12961 taatgcgcag ttcaatctgc gagtagtccg ctgagacaat cacataatcc tctggcgcaa
    13021 taaacgcctg gcggatacga cgaccttctt cgttacgcac cggaatgttt tgcaggttag
    13081 gatcggttga cgataaacgt cccgttgcag ttactgcctg gtgataagag gtatgcacac
    13141 gcccggtttt cgggttgatc atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg
    13201 ccagaccacg atactccaga atcacttttg gcaacggata gtccagcgcc agttcttcca
    13261 gtacctcttc cgacgttgac ggcgcgccac ccggcgtttt cttcagcggt ttaataccct
    13321 gtttttcaaa cagaatggtt tgcaactgct tggtggaaga aaggttaaat tcttcacctg
    13381 caatttcatg cgctttcttt tccagctcag ccagacgcaa ggtgagctct tcagaatgat
    13441 tgtgcagcac tttcggatcg atcttcacac cgttacgttc aatgcgtgaa agcaccggca
    13501 ccagcggcat ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg
    13561 gccacatttt cagatgcaac tgcaaggtga catctgcatc ttcggcggcg taacgtccgg
    13621 cctcttcgag ggcaatctgg ttaaaggtca gttgattttt gcctttaccc gcaatctctt
    13681 caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc
    13741 cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc ccacgcagtt
    13801 caatgccgta attcgccaga ataccgcgat cgtatttcag gttttgcccg accttcagcg
    13861 ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgtga gagatttgat
    13921 cgggcgcatc aagataatca tgagcaaccg gaatatatgc cgctacgcct ggctcgatag
    13981 caaaagaaag cccgaccagg ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa
    14041 atgcaaatac cggcgctttt tccagcttcg caatccacgc tttcagtgtt tcttcatcaa
    14101 ggatagtgac gtagttgtca taagaaatca ccgttgccgt cacttctggc gcttcgtctg
    14161 caacactggt ttcctgcggc ttcgcggctg gttttgcccc tttggcctgt aaccatttgc
    14221 ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacagc cccaacaact
    14281 cttccgctgc cggttgctgc acttccagtt gttcacaggt cagctccagt tcaacatcgg
    14341 ttttaatcgt cgccaactgg tatgagagat aagcaacttc tttgttttgc tcgagcttcg
    14401 ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc tcggtataca
    14461 gcgtatccag cccgccaaga ccttgcagca atgcctgcgc ggttttttca ccgacgcccg
    14521 gtacgccagg aatgttatca gaggagtcac ccatcagcgc caggaagtcg atgatcaatt
    14581 ctggcggcac gccgtactta ttcaccacct cttccggtcc gaggatggta ttcgtcatgg
    14641 tattgataag cgtaatattt ggcgtcacca gctgcgccat atctttatcg ccagtgctaa
    14701 tcagcaccgg acgcccggct ttttcggctt cgcgcgccag agtaccgata acgtcgtccg
    14761 cttctacgcc agaaaccgcc agcagcggca gtcccatcgc tttaaccatc gcgtgcagag
    14821 gttcgatttg tgctcgcaga tcgtccggca ttggcgggcg atgtgatttg taatgttcaa
    14881 acagttcatc acgaaaggtt tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt
    14941 tatattgcat gatcagactg cgcagcatgt tgaggacacc atacatcgca ccggtcggct
    15001 cgcctgcgct gttagtcagc gggggaaacg cgtgatatgc gcgataaaga taagatgaac
    15061 catctacaag gataagtgga ttttggggga tctgaaccat aatgtccgtg cctgtttatc
    15121 agattatggg taaaggatgc cacagacagg atgaaaacat gagtttttca ccgcttttcg
    15181 tgaaaatctt ttgtagatct gctggatcgc tcgcaaatcg tcatgtggat aactttgtgc
    15241 ataaattatt tacatcgctt ttaatttatt atcatgcttt ttacagcatg atgtttgtct
    15301 ttatttttca tgcagttaaa aataaatcca tccctaataa ttgagataat gggatttttt
    15361 ctaatgtgga tagatatgaa ttatttttct ccttaaggat catccgttat ttgggtcatt
    15421 ttctgctaaa atctgcgcct tgcgcttctt tagtgcactg tctatggcta accttttgaa
    15481 taaattcatt atgacgagaa tactcgctgc gataaccctt ttgctgagta tcgtattgac
    15541 cattttggtc actatttttt gttccgtccc gatcatcatt gccgggattg taaaactatt
    15601 gctacctgtg ccagtcattt ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg
    15661 ctggtgtgaa ggtctggcgg tattactgca tctgaaccca cacctgcaat gggaagttca
    15721 cgggctggaa gggttaagta agaagaactg gtatctgctt atttgtaacc accgtagttg
    15781 ggcagatatt gtcgtactgt gcgtgctgtt tcgtaagcac attccgatga ataaatattt
    15841 cctcaagcaa cagctggcct gggtgccatt tcttggcctg gcgtgctggg cgctggatat
    15901 gccgtttatg aagcgttatt cccgcgctta tttattacgt catccggagc gacgtggtca
    15961 agatgttgaa accactcggc gttcttgtga aaagtttcgc ctgcatccca ccaccattgt
    16021 caatttcgtt gaaggctccc gtttcacgca agaaaaacat cagcaaaccc actccacttt
    16081 tcaaaacttg ttgccaccaa aggcggcagg cattgcgatg gcgctaaatg tactgggtaa
    16141 acaattcgat aaactgctta atgttacgct gtgttatccg gacaataacc gtcagccgtt
    16201 cttcgatatg ttaagcggta aactgacgcg gattgtcgta catgtggatt tacagcctat
    16261 tgccgatgag ttacatggcg actacatcaa tgacaaaagc tttaagcgcc attttcagca
    16321 atggctgaac tcattgtggc aggaaaaaga ccggcttctg acgtctctca tatcatcaca
    16381 gcgtcagaat aagtaagttt tactcgcctc tgaatgagca gaggcgagtg agtattttaa
    16441 tgaacaaaac gtccggcacg agacataaat tcttcttcgc tcatctcctg tccgttaaaa
    16501 acaactttac ccggtgtata acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt
    16561 aactgaaaca tctgacctaa tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc
    16621 gctttttccg catccattcc ttcagataaa ttaagctgtt ttgcggttta tgtgacgaca
    16681 ttaagcggta acttcacatc aaaattgagc gatttgatat ctttgtttgt ggatgatgaa
    16741 gatttggctg ggtcggcaat actgatatcc agattggcat ttagttcgcc gagtgcgttc
    16801 ttccatctta ccggttgttt aatggtcggc tcactttttt gtaataacgg taaatactct
    16861 ttgaacaatg cagcattcac ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt
    16921 gcagcaagct gcttctgcat cgcaatgtta tactggatga taaattggcg cactgctgag
    16981 ggatcaatag attcagcaat caatgaccac tcaccactac cgagttgctg attccctttt
    17041 gttaacttat caagttcgta ggtcagattg acattgatat aatctttgtc tttaacgcga
    17101 tccagcctgg tttttgcaac gaaagcatcg atttgggcaa cgtcttcccc gtgattgata
    17161 atgttaatct gattaaattt cgactcgctt tcaccaaccg gaaatttctt ttcttctaat
    17221 cgcgccagag aatcaatggt aaaacttttt gccgttactt tgttcattgg tgataattgc
    17281 agaacaatat cttcgacttt gccttcaata tccaggttag acaatgaact accatcacca
    17341 ttaaaagtaa actggccctc accaaaagaa aactttccat actctgcagg ggcaacatct
    17401 aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg tcgcttccac gggcgattta
    17461 tttttcgcgg cgataaatag cggttgtgtc agttcattat tcactacgtt cacttttgcc
    17521 gctgccaatg ctgggatgac attaccatgc attaacattg tgatgggcaa cggaccgtgc
    17581 tcaacatcca catcaaatac aactttctgg cctggcttga tattgagatc gggtgcaccg
    17641 ttatcgaatg tcatttgcat ttgaatacgg gaattaaaaa agcctttgtc aaagttttta
    17701 taacttaacg tcatatcgta ggcatgttca cctttctttt tcgcatcgtt aaaatcttta
    17761 ataaactttt cgataccagg ctgaatttgc gtacctgtgt accatgtgcc accaccccag
    17821 attacggcta acgcaacaat aacacctgta gctgacttac gtatcatata aatattccct
    17881 gataaaacag caaaactttg aatatccact tatgctgaaa taatgtccag cggcaggatg
    17941 cattatcaga atgtatttat atttattaaa taataaaaaa agcccgtgaa tattcacggg
    18001 ctttatgtaa tttacattga attatttttt ctcggacaga tatttcaccg tatcagcata
    18061 ctgctgaaca aaaacatcca tattgctggt atccataccc tgcggattca gctgatattt
    18121 accgttaaca aacatcgccg gaacaccacg caattgcaca tcagctgcgg ctttttcctg
    18181 ctgagcgacc agagatttca ccacgaagct gttccacgcc gcgtcgtact cttcaccttt
    18241 aatacctgca ttgataaata catcgcggat atcagatgct gaacgaatgg tctgggtttt
    18301 ctgtacgcct tcaaacagcg gaacagtcac tttgtcttcc acgcccagcg ccatcgccac
    18361 agcccatgcc tgagtcagat ctttgcccag gtcaccaccc atgaagttga cgtggtattt
    18421 agtcatcttc acgccttccg gcagtttttt cttcacatta tcagaaatat gcagaacttc
    18481 ttcaaactga tagcagtgcg ggcagaagaa agagaaaaac tccagcactt gcggcgcgcc
    18541 agctaccggt ttttccaggg tagtgtactg tttaccatct tcatactgcg ccgccgatgc
    18601 gctaaacgct aaaactaaac cagccagcgc cagccaaatc tttttcatga tctactctct
    18661 ccgattaata cataggtgtt aattgcaaag ggggttcttg tagaactttt gcctgttcta
    18721 taaaagtcgc cgtctgtcgt agccagtaat cttccccggt taaccacggg aaatttttcg
    18781 ggaacgcggg atcagcccaa tgccgcatta gccaggcaag ataataaacc aaacgcatgg
    18841 cgcgtaaagg ttcaatcagt ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag
    18901 cttcaataat agtttccagt tgcatccgct gctgggcttt atcgccattg agcaacatcc
    18961 acaaatcctg aatagctgga ccattacgtg catcatccag atcaacaaac attggaccat
    19021 cgcgccagag aatattcccg gcgtggcagt ctccatgtag ccgcagaacg gtgaaatctt
    19081 cccgccagtg tgctgtaacg gcggcgatca gctcatctgt cgctttcaga aatgccgctt
    19141 tcaacccgga aggtatcagt gtagcgtcct caaacagctt gcgaggctca atgagatatt
    19201 cattcagacc gatggtctgg cgatgaataa aaagctgttt gcgccccgtc tggtgcatac
    19261 gccctaaata acgcccaacc gcttccatct gatcgatatt atcagcttcg aactggcgac
    19321 caccgacgct tggaaaaaca gcgaaataaa atccctgatg attcaataaa gtctgaccgt
    19381 taaaggccac aggtgctgcg accggaactt catcatttac cagctgcaac gcaaattgat
    19441 gttcttcgag aatttgatcg gctgtccaac gttcagggcg ataaaatttg acgacaaaac
    19501 gtcgacgctc ttcgtcctga aattgataga cacggttttc atagctgtta agcggggtaa
    19561 gaccggaatc cacccggatc ccttgctcaa acagagcgtc catgatggta tccgggtgta
    19621 gtgtctggaa agtaaaagcg ctgttgttca tcccatcatc cggaaaacat tacgaatgat
    19681 gaaggatatc atttcacgat gattttggtg gcgccgctta caagctttta ctctttaatt
    19741 acgccgcgag cgcgtaacag cgccgttttg aagtcttctt cataatcttt ctgcaaaccc
    19801 gggatcaccg catctttcgc ggaatcacgc attttcaggt gatagatcag aatgtcatcc
    19861 gtcaaatccg ccagttcgcc gtcaaaacct gactctttcg ccagtttctg caaaaattgc
    19921 aacaagttaa agtctggctc tttttgccag gctggctgga ggagttcaat aacttcattc
    19981 agacgtttac atttcatagt aatgctcctt actcttgaga cagacacgtt agcagggtca
    20041 atcccacaat aaaagaggcg atatcggtga atctgatgac gacgataaca ggcgttgtgc
    20101 tggcaggcgg taaagccaga cgaatgggcg gcgtagataa aggattgctg gacttaaatg
    20161 gcaaaccgct gtggcaacat gtcgctgacg cgcttaagac gcagctctct cacgtcgtgg
    20221 ttaatgctaa tcgtcatcag gaaatctatc aggcaggcgg tctgaaagtg attgaagatt
    20281 cactggcgga ttacccaggc cctctggcag gaatgctttc agtaatgcag caggaagctg
    20341 gtgagtggtt tttgttttgt ccgtgcgata cgccttacat tccccatgat ttagccgccc
    20401 ggcttaatca tcagcgcaaa gatgcgcctg tcgtgtgggt ccatgacggt gaacgcgatc
    20461 acccgactat tgctctggta aaccgcgcta ttgagccttt attactggaa tatctgcaag
    20521 caggagaacg ccgggtaatg gcatttatgc gtctggctgg cggtcatgcg gttgatttca
    20581 gcgatcataa agatgcattt gttaacgtga atacgccaga ggagcttgcc cgatggcagg
    20641 aaaaacgatg ataccgttac tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct
    20701 tttgaaaaaa ctgatcccgg cattatgcgc cagagggatc cgtccggggc tgattaagca
    20761 tacgcaccat gatatggatg ttgataagcc aggcaaagat agctatgagc tgcgcaaggc
    20821 tggcgcggca caaaccatcg ttgccagcca gcaacgatgg gccttgatga cggaaacacc
    20881 agacgaagaa gagctggatc tacattttct cgcaagtcga atggatacct caaagctgga
    20941 tttgattctg gtcgaagggt ttaagcatga agagatcgca aagattgtgc tgtttcgcga
    21001 tgcagccgga catcgacctg aagaattagt gatagacagg catgttattg ctgtagccag
    21061 tgatgtgccg cttaatcttg atgtcgcatt actggatatt aatgatgttg aggggctggc
    21121 tgattttgtg gtggagtgga tgcaaaagca gaacggataa gcaagagtag gctggatcag
    21181 acgcagccag tgtcgcatcc gacctttttc tgcaccagcg caaaaaggcc cgactgggcc
    21241 t
//
LOCUS       DAJTRQ010000063        20988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000063 DAJTRQ010000000
VERSION     DAJTRQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..2633)
                     /locus_tag="OO332_002380"
                     /pseudo
     CDS             complement(1..2633)
                     /locus_tag="OO332_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380005.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            complement(3093..3266)
                     /locus_tag="OO332_002381"
     CDS             complement(3093..3266)
                     /locus_tag="OO332_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942830.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3384..4577
                     /locus_tag="OO332_002382"
     CDS             3384..4577
                     /locus_tag="OO332_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5942831.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4775..6028)
                     /gene="glyA"
                     /locus_tag="OO332_002383"
     CDS             complement(4775..6028)
                     /gene="glyA"
                     /locus_tag="OO332_002383"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5942832.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6355..7545
                     /gene="hmpA"
                     /locus_tag="OO332_002384"
     CDS             6355..7545
                     /gene="hmpA"
                     /locus_tag="OO332_002384"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5942833.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="OO332_002385"
     CDS             complement(7590..7928)
                     /gene="glnB"
                     /locus_tag="OO332_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5942834.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="OO332_002386"
     CDS             complement(7989..9323)
                     /gene="glrR"
                     /locus_tag="OO332_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5942835.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="OO332_002387"
     CDS             complement(9313..10026)
                     /gene="qseG"
                     /locus_tag="OO332_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5942836.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="OO332_002388"
     CDS             complement(10191..11618)
                     /gene="qseE"
                     /locus_tag="OO332_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5942837.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12176..16063)
                     /gene="purL"
                     /locus_tag="OO332_002389"
                     /gene_synonym="purI"
     CDS             complement(12176..16063)
                     /gene="purL"
                     /locus_tag="OO332_002389"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5942838.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16321..17877
                     /gene="mltF"
                     /locus_tag="OO332_002390"
     CDS             16321..17877
                     /gene="mltF"
                     /locus_tag="OO332_002390"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5942839.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="OO332_002391"
     CDS             complement(17874..18410)
                     /gene="tadA"
                     /locus_tag="OO332_002391"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5942840.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="OO332_002392"
     CDS             complement(18435..19070)
                     /gene="yfhb"
                     /locus_tag="OO332_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5942841.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19279..20127
                     /locus_tag="OO332_002393"
     CDS             19279..20127
                     /locus_tag="OO332_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5942842.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20466..20988)
                     /locus_tag="OO332_002394"
                     /pseudo
     CDS             complement(20466..20988)
                     /locus_tag="OO332_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         5039 a         5672 c         5345 g         4932 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccag agtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtcctga ctggcatacg ccaccgcttg cgggaaatct
     1741 gcgcggttat attccagcat cgccagcgcc aggttacagc gataatccag cgggtccagc
     1801 gccacgccgc gcaggtagta atcgaacggt gaacggctgg cgtgatgata ttgctccaga
     1861 tgctgaccga taaaccaggc ttcatctgta ctggtaatgt cttgtgctgc cagtggcgct
     1921 ttggcgacgt ccggcaacgg caactcctgc ggctgatgtt cctgataact cagtacaata
     1981 ttgccgtcgg catcggagag ttcaatggtc agtctgtcag gattgatacc gtgcaacacg
     2041 ccctggatgg cggtcgctgg tgtcagggcc acggcatcat cgagcaacgc gttgcatttg
     2101 ccgatttcgc ggatcgccag acggtatccg ttcaacggag agatggcata cagcccccac
     2161 tcaatccccc gctcactacg ctggagtttt atcaccgcat cgcgggaggc attttgcacc
     2221 atgcacagag aatgataagg caggaaatac tgctcgaaac gcttctcttc gtaagcatca
     2281 agccaggtaa aatcaggctg gttatcggca aaaataccgg tcatcagttc gatatacggg
     2341 ccgttattgt cggtcagact cttatcccac gcctggccaa attcactgtg tccccaactc
     2401 cactgttttt tacctggcgc aatatggtgg ttggcaacgt gcagcaaacc gccatcttca
     2461 tcgtgacacc acgcgccgac aaaatcgtac tgcgattttt cggccatata tgaggttgga
     2521 acaggcacat ttttatagcg agaaatgtcc actccggcgg agtagtccac tttgtagtaa
     2581 gtgccggtgg cgatggggaa agcggagacg gcccgtttgc cgtgatcaaa caccgcagtc
     2641 acatccggtg ggaaaacgct ctgatgccct tcaccccctt tcactgccgg gttggcccac
     2701 cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc ggctggcgat ttccagtgcc
     2761 gcccggtcag ggcgcagggt gaaacctgtc atcacctgta aaccatgcat cggctccgtt
     2821 tcgccgaccc acaccgtctg tgcaccgtct tcatgggctt cgagggtgaa atcaacgggc
     2881 ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact caatcccgcc ggagatccac
     2941 ggtcccagca gccccaccag cgcaggttta atgacttcat tgtgatagac aaaatcgcgc
     3001 tgtttcactt tatcccatgc gcgatgcacc cgaccgccca gttccggcag gatcatcact
     3061 ttgatgtagt cgttttccag ccacaccgcc tgctaggatt tcagggtttt ctgctcgctc
     3121 agcgtatcgg tcacgccgta gggataaacc gcgccggacg atccctgata aacgcgattt
     3181 tccaggaaca tgggatgtat atcctgcggc ccggtttcat aggtcgggat ctcaacgcgc
     3241 tcttgccaca cttttactgg agtcatggtg ccctcaatat taacaagaca tactgaatta
     3301 aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt tcgcggaata atcacgcaat
     3361 taactaaaca aggtttagtg aagatgagag cctgcattaa caatcaacag attcgccacc
     3421 ataacaaatg cgtgattctg gaactgctgt accggcaaaa gcgcgccaat aaatcaacgc
     3481 tggcccggct ggcgcaaatt tcgattccgg cagtcagtaa tattttgcag gaactggaaa
     3541 gcgaaaaacg ggtggtgaat attgacgatg aaagccagac gcgcgggcat agtagcggta
     3601 catggctgat tgcgccggaa ggtgactgga cgctgtgcct gaacgtgacg cccaccagta
     3661 ttgagtgtca ggttgctaat gcttgtttaa gtccgaaagg cgaatttgag tatttacaga
     3721 ttgatgtacc gacgccgcag gcgctgctgt ccgaaatcga aaaatgctgg catcgccacc
     3781 gtaaattgtg gccggaccgc accatcaatc tggcgctggc aatccacggg caggttgatc
     3841 cggtgaccgg cgtatcgcaa accatgccgc aagcgccgtg gacaacgcca gttgaggtga
     3901 agtatctgct ggaagagaag ctgggcattc gggtgctggt cgataatgac tgcgtgatgc
     3961 tggcgcaggc ggagaaatgg caaaataatt cgcaggaacg ggatttctgc gtgatcaacg
     4021 ttgattacgg cattggctcg tcgttcgtga ttaacgagca aatttatcgc ggcagtttgt
     4081 atggtagcgg acagattggt cacaccatcg ttaatccgga tggcgtcgtc tgcgactgtg
     4141 gacgttacgg ctgcctggaa accgtcgcct cgttaagtgc attaaaaaaa caggcgcggg
     4201 tatggctaaa atcacaaccg gttaatactc agcttgatcc tgaaaaactg acaaccgcgc
     4261 agttaatcgc tgcctggcag agtggagaac cgtggatcac cagctgggtt gatcgctctg
     4321 ccaatgccat tggtttgagt ctgtataact tcctcaacat cctcaatatt aatcagattt
     4381 ggttgtacgg tcgcagttgt gcctttggtg agaactggct taatactatt attcgccaga
     4441 caggatttaa cccgttcgac cgcgacgaag gaccgagcgt gaaagcgacg caaattggct
     4501 tcgggcaatt aagccgcgca caacaggtgc tgggaattgg ctatttgtat gttgaggcgc
     4561 agttacgaca gatttgatgg cgcgataacg tagaaaggct tcccgaagga agccttgatg
     4621 atcataaacg aaaaattgcc tgatgcgcta cgcttatcag gcctacacgg agattgcaat
     4681 atattgaatt tgcaagattt tgtaggcctg agaaggtgtt catgccgcat ccaacatgaa
     4741 caacgagcac attgtcagca aatcaccgtt tcgcttatgc gtaaaccggg taacgtgcgc
     4801 agatgtcgag aactttacct ttgatgcgct cgataacggc ttcatcattg atgctgtcca
     4861 gcacgtcaca catccagcca gccagttctt tcgcttcagc ttctttaaag ccgcgacggg
     4921 taatcgccgg agtacctaca cgaataccgg aggtcacaaa cgggctcttc ggatcgttcg
     4981 gtacgctgtt tttgttgacg gtgatgttag cacggcccag agcggcgtct gcttctttac
     5041 cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagtg ccgccggaaa
     5101 ccactttgta gccgcgctcg aggaacactt ctaccatcgc tttagcgttt ttcgcgacct
     5161 gctgctggta agttttgaac tcaggctcca tcgcttcttt cagagcaacc gctttaccgg
     5221 cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag ttcagttttt
     5281 tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga cccgccaggg
     5341 ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg tagacgccag
     5401 cagcaaccag gcccgcaacg tgcgccatat caacgaacag ataagcaccg atgctgtcag
     5461 cgatttcacg cattttcgcc cagtcaacca cgccggaata tgcagagaaa ccaccgataa
     5521 tcattttcgg cttgtgttct ttggcttgtt tttccagatc ggcgtaatcg atatgaccgg
     5581 tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta accggagaac
     5641 cgtgagtcag gtgaccgcca tgcgccaggt tcatacccag aacggtatca cctggttcca
     5701 gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg acgttagcgt
     5761 agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg atatcaacat
     5821 actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg ttggtcagct
     5881 gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg atcagttcga
     5941 tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg gcatcataat
     6001 cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa caataaaatt
     6061 tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga atcattgtgc
     6121 gataacaggt cttgacaaag gaatttacgc aaacgattac cttaaggcta cgcaaggctt
     6181 tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg atgcaaattt
     6241 ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg cagggctatt
     6301 ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaggaaga ccatatgctt
     6361 gacgctcaaa ccatcgctac agtaaaagcc accatccctt tactggtgga aacggggcca
     6421 aagttaaccg cccatttcta cgaccgtatg tttactcata acccagaact caaagaaatt
     6481 tttaacatga gtaaccagcg taatggcgat caacgtgaag ccctgtttaa cgctattgcc
     6541 gcctacgcca gtaatattga aaacctgcct gcgctgctgc cagcggtaga aaaaatcgcg
     6601 cagaagcaca ccagcttcca gatcaaaccg gaacagtaca acatcgtcgg tgaacacctg
     6661 ttggcaacgc tggacgaaat gttcagcccg gggcaggaag tgctggacgc gtggggtaaa
     6721 gcctatggtg tactggctaa tgtatttatc aatcgcgagg cggaaatcta taacgaaaac
     6781 gccagcaaag ccggtggttg ggaaggtact cgcgatttcc gcattgtggc taaaacaccg
     6841 cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg acggtggcgc agtggcagaa
     6901 taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg aaggtttccc gcatcaggaa
     6961 attcgtcagt actctttgac tcgcaaaccg gatggcaaag gctatcgtat cgcggtgaaa
     7021 cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc acgccaatgt tggcgatgtc
     7081 gtgaaactgg tcgctccagc aggtgatttc tttatggctg tcgcagatga cacaccagtg
     7141 acgttaatct ctaccggtgt tggtcaaacg ccaatgctgg caatgctcga cacgctggca
     7201 aaagcaggcc acacagcaca agtgaactgg ttccatgcgg cagaaaatgg cgaagttcac
     7261 gcctttgctg atgaagttaa ggaactgggg cagtcactgc cgcgctttac cgcgcacacc
     7321 tggtatcgtc agccgagcga agccgatcgc gctaaaggtc agtttgatag cgaaggtctg
     7381 atggatttga gcaaactgga aggtgcgttc agcgatccga caatgcagtt ctatctctgc
     7441 ggcccggttg gcttcatgca gtttaccgcg aaacagttag tggatctggg cgtgaagcag
     7501 gaaaacattc attacgaatg ctttggcccg cataaggtgc tgtaatttga tgttgccgga
     7561 tggaaacatc cggcaaccct tgacgcggtt taaattgccg cgtcgtcctc ttcaccggta
     7621 cggatgcgaa tgacccgagc cacgtcaaag acgaagattt taccgtcacc gattttgccg
     7681 gtttgcgccg tgcgaataat ggtatcgaca caggtatcga caatgtcgtc cggtacgaca
     7741 atctcaattt tcactttcgg cagaaaatcc accatatact ccgcgccgcg gtacagctcg
     7801 gtatggcctt tctggcgacc aaagcctttc acttcggtca ccgtcatgcc ggtaataccg
     7861 acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg gttttataat cgcatcaatc
     7921 tttttcatgc tattccttga aaaggttgtc tgtcttttga tctgctaaac gtaacacata
     7981 acgccaattc attccttgaa atcgtttgca tccagctcgt gtcgggaaag cagtttataa
     8041 aattctgtcc ggttgcgccc cgccattctc gccgcgtggg tgacgttgcc tttggtgatt
     8101 tgcagcagct tacgcaaata gttgagttca aaatgattac gtgcctcaac aaaggttggc
     8161 agcgccgtat tttcaccctc cagcgcctgc tccaccagcg catcactaat caccggagat
     8221 gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact ggcgcacatt acccggccag
     8281 ctcgcggtca tcaggcgttt catcgcatcg gtagagaacg cgcggacaaa cggtttatgt
     8341 cgctctgccg cctggcgcaa caggtgattt gccagtagcg gaatgtcttc tgtgcgctcc
     8401 gccagtgccg gaattttcag gctgacaacg ttgaggcggt aatagagatc ttcgcggaat
     8461 tccccgcgcg ccatcgcttt tgacagatca cggtgagtgg cagaaataat ccgcacattg
     8521 atatcaatat cgcggttact gcccagcggg cgcactttac gctcctgcaa cacgcgcagc
     8581 agtttgacct gtaacggcgc aggcatatcg ccaatttcat cgagaaatag cgttccgcct
     8641 tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc cagtaaacgc gccacgcgca
     8701 tgaccaaaca gctccgactc cagcaattgc tcgggcaatg cgccacagtt aatagcaata
     8761 aatggtttgc tgttgcgcgg gctggcgttg tggatagcct gggcgaaaat ctctttcccg
     8821 gtgccgctct gaccgttaat caaaacgctg acgtctgatt gcgccaccag ccgcgcctgt
     8881 tccagcaaac gcagcatcag cgggctgcgg gtgacaattg cctcgcgcca gcgttcatcg
     8941 gtggctggcg cggattgctc cagcgcatcg tcaattgcct gatatagcgc atctttatcg
     9001 acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg cagcaacggc atcggggata
     9061 gaaccatgcg cagtaagaat aattactggc attcccggct gcactttctg gatttcagca
     9121 aatagctgca taccgtccat ttcatccatc cgcaggtcgc tgataactaa atctactttt
     9181 tcgcgattca gtacccgtaa tccttcagcg ccactttccg ccgtgaccac actgtagcct
     9241 tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg gatcgtcatc gaccaataat
     9301 aaatgcgcag gtttatggct catcaggagt gacctcatgg gtggatggcg cgggcttttc
     9361 actctcgtgc ggcgtatccg gcgagaaatt tccagccggt ttgcgggtcg agagctggcg
     9421 ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt tccagttgct gctgtagaac
     9481 gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg gactgctgga gtttactgta
     9541 acgttggcgc tcttccgcca gttgcagttg cagcgcctga ccgtcgcgcc agagttgata
     9601 cagtgggcga acctgtgccg ggatctcggt acttaacgct tcaatacgcg cgaccagctg
     9661 gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa atcccttgtt taaaggtatt
     9721 ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc gactgcgcag gcattaaacg
     9781 atcagcacaa tccatcgccc gcagccagta aagcggattg gtttcggttg atttaccttg
     9841 cagcgcccag atgtcgctac attcagtaga aagatagtca gccagttgat aaaccggaat
     9901 tttttcttct gctggcgtat caatggctgg cttattgtga ttctgcacgc aacccagcaa
     9961 tgccagacat ggcagccctg ccagccacag ccgtcggggc aataatcgtt gaaaaatgtg
    10021 tcgcatattc accagactta aagcctatcc cagtgggcgt aattgttgca gacagtctgg
    10081 acatggacag cgcggagaaa ccggggcgta catatcgtac gtgaagattt cgagcactac
    10141 ccggggccga actgacaaat aaaatagcct gataggatag gctgttagca ttatttcgtg
    10201 tttttcgacg acggtaattc aatgcggaaa caaacgtctt gcccgctctc gtcgaccaga
    10261 tacagttccc cttgcatacg gcgaatacaa tccctggcaa tgcttaatcc cagaccgctg
    10321 cccttcaccg ccccttttcg ctggtggctt ccctgaaaaa agggttcgaa gatcatggcg
    10381 cgttcctctt gcggaatggg cgtgcctgta ttgatgacat caatataaac ccgcgcacca
    10441 tgtaaactgc tgcgaaggca aatgttaccg gattcagccc cgtagtgcac cgcattggag
    10501 taaagattat ccagtacgct catcagcagc attggctccg ccaagcaagc tgttgctttg
    10561 agatcgacgt cggtatgcat cattttagcc cgtgcgggca ggctatgagc agaaaccact
    10621 gtctccacca gcggtgctaa ttcaacattc tccagttcca ccgcactgtc cgcctgttta
    10681 cggttgtaat caagcagttg ttcgatcagt ttttgcaaat tgcagctgct gctatcaaga
    10741 atgctcacca cctctttttg ctctggcgta agcggcccga caacctgatc agccagtaat
    10801 tcagtgccct cgcgcatact cgccagtggc gtttttaatt catgagataa atgtcttaaa
    10861 aattgatggc gttgggattc cagccatgac aggcgctcac ttaaccaaag aatacgttgc
    10921 ccaaccgagc gtaactcgct cggtccacag aacgaaacgc tattgcccag agaacgccct
    10981 tcccccagac ggttgatcat gcgctcgata tttttcaccg gcccgataat catccgcgtg
    11041 aaaagtagca ccatcaccag actcaccaga aatagcacca gcgattgcca accaaaatat
    11101 tgcccacgtt cggcgatttc acgctgaagt tgctgcccac gagagaacac cactgtgcgc
    11161 gtggcctgca ccatttcggt attggcactg gcaaaggctt caagacgcgc ggcggcggca
    11221 gcatcgggac cactgttgtt acactgaagt tgggccagat tgttcaaatc ctgacgcaat
    11281 gcctggtaaa gtttatcgtc tggcagtacg cctgcgtggg catcgagcat ttcgctgtaa
    11341 cgcttgcgct ggctttgata aaccttcgca agcgtagggt cgtccagcac gcaatactga
    11401 cggtagctac gctccatctc cagcgccgcg ttggtcatcg cttcactacg ccgggcatca
    11461 ataagcgtag tgcggttaac cagcgccgcc tgatcgctaa gcgcattcag gctttgccag
    11521 gcttgccatg ccagcaccaa caggggtagt agaattagca aaaatgccag cattaccagt
    11581 tgtcgtaatg agcggggaaa aacgggccag cgtttcaagg tgttactctc gtcagacgcg
    11641 aatagcctga tgctaaccga ggggaagttc agatacaaca aagccgggaa ttacccggct
    11701 ttgttatgga ataaggcggt gcctaactcg acgtttcgcc cgatggttga tatagctacg
    11761 ctgatatcag aagttggacg gcaggcacct tgttgtgcgt cattcgaatt ttatgtagca
    11821 cgtcccgaag gggctgacat aagtcggtga atgagccact gcttactatt atgcagtaac
    11881 tgtgccaata aagaaaatag tttggtaacg tactgattat acgttgcttt gagagattta
    11941 tggcttcttc gctatttgaa tgtaaatcag ccactgtgtc gctaaaaaga gacaacttaa
    12001 gataaactta ttagattaca ataaaatcaa tcatttaagt gtcgccagaa agcgacacgg
    12061 taaaccaccc attgtcgtga tttacagaca caaaaaagct cccagagttg ggagcttatg
    12121 atagtggttg gtgcttaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    12181 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    12241 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    12301 tgactcgacc actttcagtc gtaaccgccg taataccgtt cggtgaaccg ttcgggttag
    12361 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    12421 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    12481 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagagac gggctttggg
    12541 ttacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg
    12601 gccacaactc gctccccggg atcagttcac gcaggttaga catcatctgg caaccgttac
    12661 atacccccag ggccagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac
    12721 ggtcattgaa caggatagac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    12781 agaaacctcc gcacgcgacc agggcgtgaa aatcctccag tcccgtacgt ccagtcagca
    12841 ggtcgctcat atgcacgtcg atagcatcaa agcccccacg ttggaaagct gccgccattt
    12901 caacatgcga gttcacgccc tgctcacgca gtacggcaac tttcggacgt gcgccagtgg
    12961 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagttttaca ttcaggcccg
    13021 gatcggcgtc gttagatttc gcctgatgct cctgatcggc acactccggg ttgtcacgca
    13081 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtagtg cggctttcgc
    13141 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa
    13201 cataatggac gcaatccgca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    13261 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    13321 cgtcacccag agaggcgata tccgcgtcaa tgccacaatg acctgcaaag gccatttccg
    13381 ccagcgttac cagcaggccg ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg
    13441 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg gacaacatcg cgtacatctg
    13501 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    13561 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtacaaagc tgcggcgtga
    13621 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    13681 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    13741 ccaccgggat cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt
    13801 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    13861 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    13921 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    13981 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    14041 cctgccacgg ccccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccga
    14101 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    14161 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    14221 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    14281 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    14341 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    14401 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    14461 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    14521 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    14581 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    14641 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacca gacgccatgg
    14701 aagacgccgc gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    14761 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    14821 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt
    14881 tttcttcata agtacggaag tagccgttca gtgccggacg accaaattcg ttgttaaacg
    14941 ccgcaccgcc cagcgggcct tcggtcatga tgtccagcgc ggtaacaatg cgctcaggct
    15001 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gatacggaga
    15061 aaccaaccaa accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    15121 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    15181 tttcgacttt catcaggatg tgcgccggtt cctgatggaa attgtagcgg cccgtttcgt
    15241 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    15301 cagagagaac gtgatctggc gtggtttcga aagtattttt gatcatcctg aacagcgatt
    15361 tcggctgttg ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct
    15421 cagagttcgc ctgagcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    15481 tgaaagcatc ctgcagatag tcaatttcat cttccgccag agccaggcca agacgcaggt
    15541 tagcgtcgat cagcgcctga cggccctgcc ccagcaaatc aacgcttgtg accggagtcg
    15601 gttgatggtg ggcaaacaac tgctctgcat catctaaatc aaaaaagacc gtttccatca
    15661 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    15721 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    15781 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    15841 ggagtttgcc ttgcggggcg tggctggcga gtgccggacc atatttcagc aggcgttcaa
    15901 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    15961 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    16021 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    16081 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    16141 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    16201 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    16261 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    16321 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    16381 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    16441 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    16501 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    16561 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    16621 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    16681 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    16741 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggtg
    16801 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    16861 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    16921 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    16981 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    17041 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    17101 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    17161 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    17221 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    17281 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    17341 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    17401 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc
    17461 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    17521 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    17581 aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat
    17641 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    17701 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    17761 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    17821 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    17881 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    17941 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    18001 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    18061 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    18121 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    18181 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    18241 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    18301 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta
    18361 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    18421 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    18481 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    18541 cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    18601 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    18661 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    18721 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    18781 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    18841 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    18901 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    18961 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    19021 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    19081 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    19141 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    19201 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    19261 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt
    19321 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    19381 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    19441 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    19501 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    19561 ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgcta
    19621 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    19681 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    19741 atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta
    19801 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    19861 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    19921 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    19981 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    20041 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    20101 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    20161 gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga
    20221 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    20281 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    20341 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    20401 gtgacataca acattaactt ttcagtcaac cgtagcacgt ccttgtatac gccgtgtctg
    20461 cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag
    20521 atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat
    20581 tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc
    20641 agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc
    20701 acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga
    20761 aagttgatgc ctcgttcgcg caactcctcc accagcacga caagatgccg catactgcgc
    20821 cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cctgagcagt
    20881 tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg
    20941 catcctgcac agttcaacgc attacgttgt agatcggtat tctggtca
//
LOCUS       DAJTRQ010000064        20695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000064 DAJTRQ010000000
VERSION     DAJTRQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..419
                     /locus_tag="OO332_002395"
     CDS             <1..419
                     /locus_tag="OO332_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942843.1"
                     /translation="ELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQAL
                     PAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQ
                     APLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            658..717
                     /locus_tag="OO332_002396"
     CDS             658..717
                     /locus_tag="OO332_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5942844.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1034..1093
                     /gene="ibsE"
                     /locus_tag="OO332_002397"
     CDS             1034..1093
                     /gene="ibsE"
                     /locus_tag="OO332_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5942845.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1213..2646)
                     /gene="hldE"
                     /locus_tag="OO332_002398"
     CDS             complement(1213..2646)
                     /gene="hldE"
                     /locus_tag="OO332_002398"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5942846.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(2694..5534)
                     /gene="glnE"
                     /locus_tag="OO332_002399"
     CDS             complement(2694..5534)
                     /gene="glnE"
                     /locus_tag="OO332_002399"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5942847.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(5557..6858)
                     /gene="ygiF"
                     /locus_tag="OO332_002400"
     CDS             complement(5557..6858)
                     /gene="ygiF"
                     /locus_tag="OO332_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5942848.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7100..7720
                     /locus_tag="OO332_002401"
     CDS             7100..7720
                     /locus_tag="OO332_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5942849.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7784..9022
                     /gene="cca"
                     /locus_tag="OO332_002402"
     CDS             7784..9022
                     /gene="cca"
                     /locus_tag="OO332_002402"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5942850.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9185..10006)
                     /gene="bacA"
                     /locus_tag="OO332_002403"
     CDS             complement(9185..10006)
                     /gene="bacA"
                     /locus_tag="OO332_002403"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5942851.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10097..10465)
                     /gene="folB"
                     /locus_tag="OO332_002404"
     CDS             complement(10097..10465)
                     /gene="folB"
                     /locus_tag="OO332_002404"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5942852.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10570..11187
                     /gene="plsY"
                     /locus_tag="OO332_002405"
     CDS             10570..11187
                     /gene="plsY"
                     /locus_tag="OO332_002405"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5942853.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11200..12131)
                     /gene="ttdR"
                     /locus_tag="OO332_002406"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11200..12131)
                     /gene="ttdR"
                     /locus_tag="OO332_002406"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12338..13181
                     /gene="ttdA"
                     /locus_tag="OO332_002407"
                     /pseudo
     CDS             12338..13181
                     /gene="ttdA"
                     /locus_tag="OO332_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13178..13783
                     /gene="ttdB"
                     /locus_tag="OO332_002408"
     CDS             13178..13783
                     /gene="ttdB"
                     /locus_tag="OO332_002408"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5942854.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            13831..15228
                     /gene="ttdT"
                     /locus_tag="OO332_002409"
     CDS             13831..15228
                     /gene="ttdT"
                     /locus_tag="OO332_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5942855.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15271..16284)
                     /gene="tsaD"
                     /locus_tag="OO332_002410"
     CDS             complement(15271..16284)
                     /gene="tsaD"
                     /locus_tag="OO332_002410"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5942856.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            16522..16737
                     /gene="rpsU"
                     /locus_tag="OO332_002411"
     CDS             16522..16737
                     /gene="rpsU"
                     /locus_tag="OO332_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5942857.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16848..18593
                     /gene="dnaG"
                     /locus_tag="OO332_002412"
     CDS             16848..18593
                     /gene="dnaG"
                     /locus_tag="OO332_002412"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5942858.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18788..20629
                     /gene="rpoD"
                     /locus_tag="OO332_002413"
     CDS             18788..20629
                     /gene="rpoD"
                     /locus_tag="OO332_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5942859.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4955 a         5742 c         5297 g         4701 t
ORIGIN      
        1 ttgagttagc cgaagctaca cgtaaaaagg gtctggcgga agcagaagca caacgtgcgc
       61 tgaacgatgc tatcaacgta ctttctgatg aacaaaccag ccttaaattc aaactggcct
      121 tgttgcaggc gctacctgcg gtaatagaaa aatccgttga gccgatgaaa tctattgacg
      181 gtatcaagat tattcaggtc gatggtctga atcgtggcag cgctgcgggt gatgcaaaca
      241 cgggtaatgt ggggggcgga aacctagcgg aacaagcatt atcagccgct ctctcttacc
      301 gcacacaggc tccgctgatt gactccttgc tcaatgaaat tggcgtttca ggcggttcac
      361 tggcggcatt gacttcaccc ttaacctcaa caactcccgt cgccgaaaac gtagaataaa
      421 ttttcagcta tgaacgtccc tggcacaaaa agccggggat tttttatatc tgcgttccgc
      481 taaaaactgc aaatgatcag gtcgttgcaa cgttttgcgt gaccgctcgg ggaaggcaaa
      541 actgcctctg ggaaagcgtt gcgcgaggtc cggcgctcat caacaatcgg ggagtcagca
      601 aagggctgaa acgggaaagc ccctcccgag gaaggggcct tacagaagga aagggttatg
      661 atgaagctcg tcatcatact gattgtgttg ttactcgtaa gtttcgcagc ttattaacag
      721 ccaatcagag gggggagaaa tcctccctca cccttgttcc tttactttag gttgaaaaaa
      781 aatcagcgtc aagaaggttt cattaacaca tatccctggc ccaaaaagcc ggggattttt
      841 tatatctgcg ttccgctaaa aggtgcaaat gctcaggacg ttgcagcgtt ttgcgtgacc
      901 gctcggggaa ggcaaaactg cttcagggaa agcgttgcgc ggggtccggc gctcatcaac
      961 aatcggggag tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag gggccttata
     1021 gaaggaaagg gttatgatga agttcgtcat catactggtt gtgttgttac tgttaagttt
     1081 cccgacttac taacaactga tcagaggggg gagaaatcct cccttaccct tgttccttta
     1141 ctttaggttg aaaaaacaac agcgtcaata ggcctgcctg ctacgaagcg agatctgtga
     1201 accgctttcc ggttagcctt ttttatcctg ttggatcttc ttgatgatgt tagtggtcga
     1261 gcaaccgtct tcgaagttga gcaccagcac ttcgccgccg ttggcccaga cttctttact
     1321 cccggcgatc tcttctggtt tatagtcgcc gcccttcacc agtaaatctg gcaggatccc
     1381 ggcgatcaag cgctgcggcg tgtcttcttc aaacgacacc acccagtcga ccgcttccag
     1441 tgcgcccagc acaatcatac gctgttcgag cgggttaacg gggcgggaat cccctttcag
     1501 ccgtttggtg gaggcatcgc tgttcacagc gacaatcaag cggtcaccca gtttgcgggc
     1561 atttgccaga tagaagacgt gtccggcgtg caggatgtca aagacgccat tggtcatcac
     1621 cactttttca ccgcgtttac gcgctgccgc tacggccagc ttcagttcct cttcggtcat
     1681 cacgccaaag ccggtatctg cgcgcccacg cactgcattt tccagctcga tcggcgaaac
     1741 cgtggaggtt cccagtttgc cgaccaccac gccagccgcc gcattggcaa agaagcaggc
     1801 ttcttccagc gaattacccg ctgccagcgt tgccgccagg acgccaatca ccgtgtcgcc
     1861 cgcaccggta acgtcataca cttcctgcgc ctgggttggc atatgcagcg gcgctttacc
     1921 cggttgcagc agcgacatac cctgttcgga acgggtcact aacagagccg agagttcgta
     1981 atcggcaatc agtttcatgc cgcgctcaac aatctcttct tcggtcttac atttaccgac
     2041 aacagcttca aattccgaga gattcggtgt cagcagcgta gcgccgcggt agcgctcaaa
     2101 atcggtacct tttggatcaa tcagcaccgg aacgcccgct ttacgcgcca gttggatcat
     2161 ctgctgtacg cttgccagcg cacctttggc gtagtcagaa agcaccagcg cgccaatcga
     2221 actcagcgcc tgattaatcc gctcgtgcag cggctgcgga tcaacacctt cgaaaccttc
     2281 ttcaaaatcc agacggatca gctgttggtt gcgggaaagt acccgtaatt tggtgatggt
     2341 cggatgcgtc ggtacagaaa cgaagtcgca tttgacgttg acgtcggcca gagatttact
     2401 cagcgcgcgc gctgcatcgt caatgcccgt caacccgacc aggcgtgcat tagcaccgag
     2461 agaagcgata ttcatcgcca cgttagccgc gccgcccgga cgttcttcga tggtattcac
     2521 tttaaccacg ggcaccggcg cttccggcga gatacgactg gtggggccgt accagtaacg
     2581 atccagcatc acatcaccaa ccaccatcac tcctgcacgt tcaaactctg gcagcgttac
     2641 tttcattcct gtctcctgag agattcaaaa tttgcgcgcg ataataccat acttcattct
     2701 tccaccagcc acttctgcca gcttgcccgc accagatcac gctctgcggt gaagcaatcc
     2761 tccgacacat ggcccggcaa ttcctgtaat gccagatgat gaagttcatc gcgcagcgtc
     2821 gtgtaagcac gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt ttgcgccagc
     2881 agctccagaa tacgcacgtt gtctgaccag cgcgttaact tcggtttttc atgagcgtag
     2941 cgcaacacca gatattgggt aataaattcg atatcggtaa ttccgccttc atcggctttg
     3001 atatcaaagc gattacgatg tttattgccg agatgagcgc gcattttctc gcgcatttcc
     3061 cgcacttccg tttgcagagt tttaccttct cgcggcagtg tcataatctc acgacgcacc
     3121 gcgtcaaagt gcgcagtgag ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc
     3181 tgatgttccc acgtccaggc ctcgtttttc tgataatcgg caaatgctgc tgcggatgtc
     3241 accagcattc ccgccgcccc ggacggacgc agtcgagcat ccacttcata caaaatgccg
     3301 gaagaggtac gcgtactgaa cagatgcata atgcgttgcg ccagacgcag ataaaactgc
     3361 cgcccgtcga tttcccgctc gccgtcagtc atcgcatcca ttgggcaatc atggaggaag
     3421 ataaggtcaa gatcggaact gtagcctaac tcccagccgc ccagcttgcc gtagccgacc
     3481 accgcaaaac cgcgcccttc acgttcgttg aggtgatttg gcttaccgta gcgggccacc
     3541 atttgacccc acgcctgctg aacgacggca tctatcatag cttctgccag ccaggttaag
     3601 tgatcgctca ctttcattac cggtagcgta ccggcgatat ccgctgccgc aatgcgtaac
     3661 agctgcgcct gtttgaactg acgcagcgcc tcaagctgtt gttcttcgtc atcttccggc
     3721 acgcgcagca aatactggcg cagttcatca cggtaggcat cggtcgccgt cggctggtaa
     3781 agggtatttg gatcgagcaa ttcatccagc aataatggat aacgcgctag ctggctggca
     3841 atcatcggcg atgcggcaca cagagaaatc aaatgtttaa gcgccgcagg gaattcactg
     3901 agcaattcta aataggttgt gcgggtaaca atccccacca gcaaggcggt aatgcgcgac
     3961 agcgtgacag cagcgtcttc acgcgcacag acatcactta gcagatgtgg catcagatgg
     4021 tcgagcacct gacgtcctcg cggcccgata gtacgcttat ccagctcttt gcggaaatca
     4081 gcaatcagcg ttaggacctg tttgcgatca tcctcgctaa gatgcgccag cactggcgta
     4141 gtgtcatctt cctgcaacgc atcctgccac agctcacgcc actgttccga cagcgactct
     4201 tcctgagttt cactttcgtc gtcgccaatc aattcattaa acacccggcg cacattggtc
     4261 atatgtgcgg tcagcgcccc ggtcagttgc ggccagtcag caaagtccat cgcccacgcc
     4321 agccgcgcac ggttaagctc atcagaagga agcgtttggg tttgttcgtc gttaatgctt
     4381 tgcagcaggt tttccagacg ccgcaggaac agatatgcca ctcgcaattg ttcagcatcg
     4441 ttttcagaaa gcagatgcag cgcggcaatg gcgctgagcg ttggcagtaa agagcgcgac
     4501 tgcagcaacg gttcgcgccc accgcgaatg agctggaaca cctgaacgat aaattcaatt
     4561 tcgcgaatgc cgcctgcgcc gagtttgata ttgtcggtca aaccacgtcg acgcacttca
     4621 cgggcaatca tccctttcat gttgcgcagc gactgaatca cgctgaaatc gatgtaacga
     4681 cggaaaacaa acgggcgcag catcgcacgc aactcgttag catagacgcc ttcgctatcg
     4741 cccataatcc gcgctttgac catcgcgtaa cgctcccagt cgcgtccctg ctcctggtaa
     4801 taatcctcca acgcggcaaa gctcagcacc agcgggccac tttcgccaaa cggacgcagt
     4861 cgcatatcca cgcgatagac gaagccatcc tgcgttggtt gatccagcac tttaatcagc
     4921 cgctgcccca tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc gccctgcgta
     4981 caaccatgtt ccggccaggc aaaaatcaga tcgatatcag aggagaaatt cagttccccg
     5041 ccgcccagct tacccatgcc taagatcagc agcggttgcg cttcgccctg cgcattgcac
     5101 ggcgttcccc actcgcggca gcaggcgtca tacagccagt cacgcgccgc aacaatcagc
     5161 gtctccgcca gataactgag ctgctgcaat atgctctctt cagtaaccag tgccagcgtt
     5221 tgcgcccatg cgatgcgcac cataatgcgc cgccggaata gccgcagctc gcgcattaac
     5281 ccggcttcgt cgctcacatt actgagcgcc tcctgcaacc acacgctgta atgttgccat
     5341 tcgtcagcct gtggcggttg gctttccagt tccgtcagcc actctggatg cgcaatcacg
     5401 ctgtcctgca caaaatcact gaaagtaagt actgacttcg cctgtgcact aagtgattcc
     5461 tcggctaaaa gctctggcag ccgctcaaca acggtctgcc agtactgctg taacggtgaa
     5521 gagagcggct tcataagcga ttttatcctt gcctgattaa cgttttccgc tgtgcaacca
     5581 gaacggttcc tgattgtttg cctcattacg gaaatgttca atttcaatgc gttgcccggt
     5641 cgcaatagcg tgacgcagcc cctgccagtt ctccagccag gcttgcgcga cgacaggatc
     5701 atagtaaccc gccagcaaca gtattgagtc aatatcacgc atcaggcgtg gcaactggtc
     5761 acggtagcga tcgcctaacg gctggcagaa aacgcttttc agttcagcgg catggcggga
     5821 aagatggata tcggcaaagc gtttgaagga gtcgctaatt ttgccctgcg ctttggcatc
     5881 taaaaacggc tgccatgctt tgcttaccag ccattcggtc aacgccagct tcgccattgc
     5941 cgtttcggta gagtagaccg ccgtcacggc agaaaccgca gaagcgatgg tcgcttcgca
     6001 ttgagtcagc aaatcacgta agtgagtgct cgctttacgc ggcacaatgc caccgaacag
     6061 catcagtgta tgacggacta ggctaatggc tgccagcacc tgctctttcg ccgcatcgtt
     6121 gccgcgtacc cacagttctt catgatactg ccattgcgct aacaccagct cgagcgccgc
     6181 ttccagcccc tgttccacat cggcttttgc cgcaacatgc aaaatggtgg tcggtttgat
     6241 ttcacgcgcc ggattgccct gcgccagatg atagccacgc gccgctttgc tcaggctgcc
     6301 ctggcgtaat ccggtttgcg ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc
     6361 gccgctaagc agctccagtt ccagctcgca gataggctca gcaaattcac ccgctttcac
     6421 ttccccctgg tcgagagcga tttcaattcg gctgccatcg atctctacta gccatttttc
     6481 gcgataaaaa tcggtgctaa acagcggctg cacgcgggaa gcgagatcgg caggcaattc
     6541 gccgttcggc cagatttccg tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc
     6601 cacgttatat tccgggcgct ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt
     6661 catctcatag cgaccgtttt cgccacgaat acgtaagccc atatcgtgcc tacgcagcca
     6721 gttatccggc gtttcgtagt aaatattcag caactgcacg gggtcatggt gctcgccgcc
     6781 cagcgtattg agatggtcac gcaacgcctc aacggcactg tgattaacaa taaactttaa
     6841 ttcgatttcc tgagccatgg cctttactta tcggttatgt cacataagtg acgatgaacg
     6901 gcgaacttaa tgcgatcttt tttgtcagta gatagtattt tgcgccaaat tgccatgcaa
     6961 cgggcaattt gacgggcgta aaagtttgaa gcagtggcaa gacctgacac agaggattcc
     7021 gattgatgtc gaatgctttg cgtagcgaca ctgatgccac tactatcgtt ccactttcca
     7081 tgacaataac gacagcctga tgccaaaatt acgcctgatc ggattaactt tactcgcact
     7141 tagcgcgact gccgtctcac acgctgaaga aacgcgctat gtttctgacg aactgaatac
     7201 ctgggtccgt agcggtccgg gagatcatta tcgcctcgtg ggcacggtta acgccggcga
     7261 ggaagtgacc ttattacaaa ctgacgccaa caccaattat gcccaggtga aagacagctc
     7321 tggccgtacc gcctggatcc cgttgaaaca acttagcact gagccaagcc tgcgctcccg
     7381 tgtgccagat ctggaaaatc aggtcaaaac cctgaccgat aaactcacca atatcgataa
     7441 cacctggaat cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat
     7501 caacgggtta aaagaagaaa atcagaaact gaaaaacgag ctgattgtcg cgcagaaaaa
     7561 ggtcgatgcc gccagcgtac agctggatga caaacagcgc accatcatca tgcaatggtt
     7621 tatgtatggc ggtggcgtgc tggggcttgg cttgctgctc ggtctggtac tgccgcacct
     7681 gatcccaagc cgcaaacgca aagatcgctg gatgaactaa atcgccttct tcgttgcact
     7741 gtcttattat taggaattgt gtatttgacg aagggatgat ggcgtgaaga tttatctggt
     7801 cggtggtgct gttcgggatg cattgttagg gctatcggtc aaagacagag attgggtggt
     7861 ggtcggcagt acgccacagg agatgctcga cgcgggctac cagcaggtag gccgcgattt
     7921 tcctgtgttt ctgcatccgc aaacgcatga agagtatgcg ctggcacgta ccgaacggaa
     7981 atccggttcc ggttacaccg gttttacttg ctatgccgca ccggatgtca cgctggaaga
     8041 tgatcttaag cgtcgcgatc tgaccattaa tgcgctggcc caggacgata acggcgagat
     8101 tatcgacccg tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc
     8161 cgcttttggc gaagatccgt tacgcgtact gcgcgtggcg cgttttgctg cgcgttatgc
     8221 ccacctcggt tttcgtattg ccgatgaaac tctgacgctg atgcgcgaga tgacccatgc
     8281 gggtgaactg gaacacctga cgcctgaacg ggtatggaaa gagacggaaa gcgccctcac
     8341 cacccgcaat ccccaggtgt tcttccaggt actgcgcgat tgcggcgcac ttcgcgtttt
     8401 attcccggaa attgacgcac tgtttggcgt tccggcacct gccaagtggc atccggaaat
     8461 cgacacgggt attcatacct taatgacgct ctcaatggcg gcgatgctga gtccgcaggt
     8521 tgatgtccgt ttcgcaactt tatgccacga tctcggtaaa ggactgacgc cgccagaact
     8581 ttggccgcgt catcatgggc atggcccggc gggtgttaag ttagtggaac aactatgcca
     8641 gcgtctgcgc gtgccaaatg aaattcgcga tttagccaga ctggtggctg agtttcacga
     8701 tctcatccac accttcccaa tgctgaaccc aaaaaccatc gtcaaattat ttgattccat
     8761 cgatgcctgg cgtaaaccgc agcgtgtcga gcaactggcg ctgaccagcg aggctgacgt
     8821 gcgcggcaga accggttttg aatcagcgga ctacccgcaa ggccgttggt tgcgcgaagg
     8881 ctgggaagtg gcgcagtcag tgccgacaaa agcggtcgtt gaagcgggat ttaaaggcgt
     8941 ggagattcgc gaggagctga cccgacggcg gattgcggcg gtagccagct ggaaggaaca
     9001 acgttgccca aagcctgact gagtataaaa ctaaacgtta gcacgaaccg tcccctcgcc
     9061 ccttcgggga gagggttagg gtgaggggaa cagaccagca ccggtgcgaa caatcacccc
     9121 caccccggcc ctctccctac agagcgaggg agaaaacccg gcctgctccc gaaccttttc
     9181 tcaatcaaaa gaacacgaca tacaccgcag ccgccacaat aaagcgataa atggcgaacg
     9241 ggataaacga aatgcgctta atcaattgca ggaaggtttt aatcgctatc agcgccacca
     9301 caaaagcggt gataaaccca acggcaaaca tcgggatatc gccggttgtc aggaagcccc
     9361 agcttttgta gagatcgagc gccgttgcgc ccatcatcat cggtaccgcc agcaggaacg
     9421 agaactcgga agcagcgtaa cggctcacgc ccatcagcat cccacctgaa atggtcgccc
     9481 cggaacggga gaaacccggc cacagcgcca gacactggaa acagccaatc ataaacgcct
     9541 gacgataggt catatcatca agacccggcg cacgcggctc tttcggcttc aggcattcgg
     9601 cggcaatcag caacaaaccg ccaacgacca gcgcatacat cacatttatc gggttaaaca
     9661 atgacttaat cgtgtcgtgg aataatagcc ccagtaccac cgccggaatc atccccagca
     9721 aaatgtggat cagcgttaaa cgacctttgc tttcaccttc gtgctgcaac gggcggccaa
     9781 agtggatgcc aatcaggcca aacagacgcc gccagaacat cactactacc gccagaattg
     9841 atcctaactg gatcacaact tcaaaggttt tcgccgtgtc gccctcaaac cccaacaagt
     9901 gaccgacaat aatcatatgg cccgtgctgg ataccggcag aaattctgtc aatccttcga
     9961 ccacacccaa tattgccgct atcagcagcg agtgcatatc gctcatcaat aaacccctaa
    10021 attattaaaa tgtaccgctt gtccgaacta ctgcagtatg accaggttat aaccgtttgg
    10081 tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca atgattacgc
    10141 caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatacgc acccacggcg
    10201 agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc accagcgcaa
    10261 aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa ctgaggcaat
    10321 ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg atatcgaaca
    10381 ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg attaccgaaa
    10441 gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa ccggatacca
    10501 cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat tttcaaaacg
    10561 gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct ctgcggctcc
    10621 atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg aaccagcggc
    10681 tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc agccgtagca
    10741 gtactgattt tcgacgttct gaaaggaatg ttgcctgtct ggggcgcgta tgaattaggt
    10801 gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca catctggccc
    10861 gttttcttcg gttttaaagg aggaaaaggc gttgctaccg cttttggagc aatcgcacca
    10921 attggctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt gctattgagc
    10981 ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta tgtctggtgg
    11041 tttaagccac aattcacctt cccggtttcg atgctctctt gcctgatcct gctgcgtcat
    11101 catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac gaaattcaaa
    11161 agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct gatagccttc
    11221 atcgggcttg cccagccgtt gctggcacca tgccgccaga aattccacgc agacacgaat
    11281 ttcatgctgc gatagagcgg ctcccggtaa acagcccaga tattggcgct ttgtgcatac
    11341 tccggcaata cttgcaccag tttgccactc tccagaaacg gcaacacatc ccactcggaa
    11401 cgcagcataa tccctttgcc ctccagcgcc cattgcaaca ctatctcgcc gctattggag
    11461 gaaagatgcc cgctaacctt caccgatttt ttctcctgac cgttccccaa ctcccatatt
    11521 ccgtgggtca tatcgcgctc tttggtcacc agacagtcat gacgacttaa ttcctgtaat
    11581 gattgtggct gcggatattt ttgcagatac tcaggtgctg cgcataatat tcttttattt
    11641 ttcgttaaca gatgcgcaat ataataatca ggaatttcgt cattaatacg aatatccaga
    11701 tcaatattat cctgcactaa atcaatttgc cgatcgaaca attcaaaatg cacctgtaac
    11761 tcaggataat tgcgcataag ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca
    11821 aaactgcaac caatacgaat catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg
    11881 accagtcgct gatattgggt gaggatttcc agcgcatgtt cgtaacaacg ttggccactt
    11941 tcagtcagcg ccacgccgcg agcggagcgg ttcagcagcg ttgttgccag cgtattttca
    12001 aggatttgga tccgcttggt gacaaatgca ggcgtttgcc caagcgtcgc cgctgcagca
    12061 ctaaaactcc cgctgtggac gatttctacc agtacctgca agtctttggc taaaggccag
    12121 ctattcagca tcgttaatta tccgcagttg tgataagcgc agtgtatttc gcaaaacatt
    12181 gccattccct gctctggcgc aatattcatt cttattttcg cgttgattca cgaactgtta
    12241 attacatatt cacggtggca aaaaatataa aaccacattt ttagtggtag tttgtggcgg
    12301 tgaatttttc cagacaaata caaaaactgg agttgccatg atgagcgaaa gtaataagca
    12361 acaggcagtg aataagttga cagagattgt cgctaacttt accgccatga tttctacccg
    12421 aatgcctgat gacgtggtgg ataaactaaa acagctaaag gatgccgaaa cgtcgtcgat
    12481 ggggaaaatt atctaccata cgatgttcga caacatgcaa aaagcgattg acctgaatcg
    12541 tcctgcctgt caggacaccg gggagattat gttcttcgtt aaagtcggtt cccgcttccc
    12601 actgcttggc gagctgcaaa gcatactcaa acaagccgtg gaagaggcaa ccgtcaaagc
    12661 gccactacgt cacaatgcgg tagaaatttt tgacgaagta aacaccggca aaaataccgg
    12721 tagcggcgta ccgtgggtca cctgggacat catccccgac aatgacgatg cggaaatcga
    12781 agtttacatg gcaggcggcg gctgcacgct acctggccgc tcgaaagtgt taatgccgtc
    12841 agaaggctac gaaggcgtgg tgaaattcgt cttcgaaaat atctccaccc tcgccgtaaa
    12901 cgcctgtcca ccggtactgg tgggcgtggg catcgccacc tcggtggaaa ccgccgccgt
    12961 actctcgcgt aaagccattc tgcgcccgat tggcagccgc catcccaatc caaaagcggc
    13021 agaactggag ctacgcctgg aagaaggact caaccgtctg gggattggtc cacaagggct
    13081 gaccggcaac agttcagtga tgggcgcatc gtcgcggcac gctgctggtt catgccgatc
    13141 tcacctttga aaatctgtct cacacccgga gcgcgttatg aaaaagatcc tgacaacccc
    13201 gatcaaagct gaagatctgc aagatattcg cgtcggcgat gtgatctacc tgaccggtac
    13261 gctggtgacc tgccgcgacg tttgtcaccg ccgtttgatc gaactgaaac gtccgatccc
    13321 ttacgatctc aacggcaaag cgattttcca cgctggcccc atcgtgcgca aaaacggcga
    13381 caaatgggag atggtctccg tcggcccgac aaccagtatg cgtatggaaa gttttgaacg
    13441 tgaatttatt gagcagaccg gcgtaaaact ggtggttggc aaaggtggta tggggccgct
    13501 gaccgaagaa ggctgccaga aattcaaggc gttacatgtg attttcccgg caggctgcgc
    13561 ggtgctggcg gcaacccagg tagaagagat tgaagaagtg cactggacag aactcgggat
    13621 gccggagtca ctgtgggtct gccgggtcaa agagttcggc ccgctgattg tctctattga
    13681 tacccacggc aacaacctga tagccgaaaa caaaaagctg ttcgccgaac gccgcgatcc
    13741 catcgtggaa gagatctgcg agcatgtcca ttacatcaaa taaccctccc ggagaggctc
    13801 acccctctcc tttttcgcag gcataacacg atgaaacctt ccactgaatg gtggcgatat
    13861 cttgcaccgc tggcggtcat cgccattatt gctctacttc cggttcccgc agggctggag
    13921 aaccatacct ggctctactt tgccgttttt actggcgtga tcgttggact gatcctcgaa
    13981 cccgtgccgg gtgccgtggt ggcgatggtg ggtatctcca tcatcgccat actctctccc
    14041 tggctgctgt tcagccccga acagctcgcc cagccaggct ttaagttcac cgccaaatcc
    14101 ctctcctggg cagtttctgg tttttctaat tcggttatct ggctcatttt cgccgccttt
    14161 atgtttggca caggctatga aaaaaccggg cttggacgcc gcattgcgct gattctggtg
    14221 aaaaagatgg ggcatcgcac gctgtttctc ggctatgcgg tgatgttctc cgagctgatc
    14281 ctggcacctg taacaccgtc caactcggcg cgtggtgcgg gtattatcta tcccatcatc
    14341 cgtaacctgc caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg cagcattggc
    14401 tcgtacatca tgtggatggg gattgttgcc gactgcgtga ccagcgccat tttcctgacg
    14461 gcgatggcac ctaacttgct gttaattgga ctgatgaaaa gcgcatctca cgccacgctg
    14521 agttggggcg actggttcct cgggatgttg ccgctcagca ttttactgct tctgctggtt
    14581 ccctggctgg cttacgtgct atacccgccg gtactgaagt ctggtgatca ggtgccgcgc
    14641 tgggcagaga cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa acggatgctg
    14701 gggctgatgg taggcgcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc
    14761 agtaataaag cggcatggaa cgttttcttc tggctggcct cgcttatcac cctcgcgacc
    14821 ggactcaaca ataccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagt
    14881 ggttattcgc cgacgatggt gatggtgacg ttgattgtgg tgttttatct actgcgctac
    14941 tttttcgcca gcgccacggc gtatacctcc gccctcgcac cgatgatgat cgccgccgcg
    15001 ctggcgatgc cggaaatccc gctgccggtg ttctgcctga tggttggcgc ggcaattggt
    15061 ctggggagca ttcttacacc atacgccacc ggacccagtc cgatttacta cggtagtggt
    15121 tatctgccaa cggcggatta ctggcgactg ggggcgattt ttgggctgat attcctcgtg
    15181 ttgctggtga ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg
    15241 ccggaaattg ctctccggca cccaatagtt ttacgcagcc ggtaactccg ccagcggcca
    15301 gcgcggacgc acgctaacgc cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc
    15361 ggcataggcg atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc
    15421 gccgcggcgt tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac
    15481 gccgcccgcc atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct tacacttaat
    15541 catcaacgta tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc
    15601 gtcggtgctg ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa
    15661 atccagcccc ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg
    15721 agccgccatt ttcgacagta acggtccgcc aggataatcc agccccagca gcttcgcggt
    15781 tttatcaaac gcttcaccgg cggcatcatc gatagactcg ccgagcagct cgtactgacc
    15841 aatgccagtc acgctgatta actgcgtatg accgccggac accagcagcg cgacaaacgg
    15901 aaattccggc gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac
    15961 cgggattgcc ggaacgttcc aggcaaacgc cagagagcgc cccacggttg cgccaaccag
    16021 cagtgcgccg actaatccag ggcctgcggt ataggccaca gcatcaatgt cttttgccgt
    16081 taaaccagac tcctttagcg ccgcctggat caacggtacg gttttacgca catgatcgcg
    16141 ggaggccagt tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa
    16201 ttggttggct aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga
    16261 agtttcaata cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta
    16321 ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc
    16381 ctcaccctta taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat
    16441 tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa
    16501 ttaatcaaag gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac
    16561 gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt
    16621 cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg
    16681 aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc
    16741 cccgagagcg ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag
    16801 gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa
    16861 tcccacgcgt attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg
    16921 cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg
    16981 agaaaacccc gtcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg
    17041 gcgcgcacgg caacgcgatc gacttcctga tgaactacga caagctcgag ttcgtcgaaa
    17101 cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc
    17161 ccagccagat cgagcgccat cagaggcaaa cgctttatca gttgatggac ggtctgaata
    17221 cgttttacca acaatcttta caacaacctg ttgccacgtc tgcgcgccag tatctggaaa
    17281 aacgcggatt aagccacgag gttatcgctc gctttgcgat tggttttgcg ccccccggct
    17341 gggacaacgt cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg
    17401 cggggatgtt ggtcactaac gatcagggac gcagttacga tcgtttccgc gaacgggtga
    17461 tattccccat tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc gtgctgggca
    17521 acgatacccc caaatacctg aactcgccgg aaacagacat tttccataaa ggccgccagc
    17581 tttacggtct ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg
    17641 aaggctatat ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt
    17701 taggtacgtc aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca
    17761 tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg
    17821 cgctgcctta catgacagac ggccgtcagc tacgctttat gtttttgccg gatggcgaag
    17881 accctgacac gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg
    17941 cgatgccact ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc
    18001 ctgacgggcg cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg
    18061 aaacgctgcg aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca
    18121 gccagcttga acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc
    18181 agctaaaacg cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag
    18241 cgacgttggt cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat
    18301 tcagagaact ggtcaacact tgtctctccc agccaggtct gacctccggg caacttttag
    18361 agcactatcg tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata
    18421 tagcagataa gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt
    18481 cgctgcttga actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca
    18541 acgaagaacg cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg
    18601 gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa
    18661 atatataagt acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt
    18721 aacggctgaa ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata
    18781 ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga
    18841 gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc
    18901 agatcagatc gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga
    18961 agcaccggat gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga
    19021 agccgccgcg caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt
    19081 acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga
    19141 catcgctaag cgtattgaag acgggatcaa ccaagttcaa tgctccgttg ctgaatatcc
    19201 ggaagcgatc acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct
    19261 gtccgatctg atcaccggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc
    19321 cactcacgtc ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga
    19381 agaagatggc gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg
    19441 cgaaaaattt gcggaactac gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa
    19501 aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca
    19561 gttccgcctg gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga
    19621 ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc
    19681 gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc
    19741 ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca
    19801 tcgcgccctg caaaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt
    19861 taaagatatc aaccgtcgta tgtccatcgg agaagcgaaa gcccgccgtg cgaagaaaga
    19921 gatggttgaa gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg
    19981 cttgcagttc cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa
    20041 attcgaatac cgccgtggtt acaagttctc cacctacgca acctggtgga tccgtcaggc
    20101 gatcacccgt tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga
    20161 gactatcaac aaactcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc
    20221 gacgccggaa gaactggctg aacgtatgct gatgccggaa gacaagatcc gcaaagtgct
    20281 gaagatcgcc aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca
    20341 tctgggggat ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga
    20401 aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcaaaagt
    20461 tctgcgtatg cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa
    20521 acagttcgac gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact
    20581 gcgtcacccg agccgttctg aagtgttgcg tagcttcctg gacgattaat cggtaggccg
    20641 gatcaggcgt tacgccgcac ccggcactag tctctctgca caaacgccac ctttt
//
LOCUS       DAJTRQ010000065        20550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000065 DAJTRQ010000000
VERSION     DAJTRQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="OO332_002414"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="OO332_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5942860.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="OO332_002415"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="OO332_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5942861.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="OO332_002416"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="OO332_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5942862.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO332_002417"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="OO332_002417"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5942863.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO332_002418"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="OO332_002418"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5942864.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4450..4815
                     /locus_tag="OO332_002419"
     CDS             4450..4815
                     /locus_tag="OO332_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5942865.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4965..5483
                     /locus_tag="OO332_002420"
     CDS             4965..5483
                     /locus_tag="OO332_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5942866.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5473..6698
                     /gene="dgcN"
                     /locus_tag="OO332_002421"
                     /pseudo
     CDS             5473..6698
                     /gene="dgcN"
                     /locus_tag="OO332_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6714..7196
                     /locus_tag="OO332_002422"
     CDS             6714..7196
                     /locus_tag="OO332_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5942867.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7273..7620)
                     /gene="rplS"
                     /locus_tag="OO332_002423"
     CDS             complement(7273..7620)
                     /gene="rplS"
                     /locus_tag="OO332_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5942868.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7662..8429)
                     /gene="trmD"
                     /locus_tag="OO332_002424"
     CDS             complement(7662..8429)
                     /gene="trmD"
                     /locus_tag="OO332_002424"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5942869.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8460..9008)
                     /gene="rimM"
                     /locus_tag="OO332_002425"
     CDS             complement(8460..9008)
                     /gene="rimM"
                     /locus_tag="OO332_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5942870.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9027..9275)
                     /gene="rpsP"
                     /locus_tag="OO332_002426"
     CDS             complement(9027..9275)
                     /gene="rpsP"
                     /locus_tag="OO332_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5942871.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9412..10773)
                     /gene="ffh"
                     /locus_tag="OO332_002427"
     CDS             complement(9412..10773)
                     /gene="ffh"
                     /locus_tag="OO332_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5942872.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10940..11731
                     /locus_tag="OO332_002428"
     CDS             10940..11731
                     /locus_tag="OO332_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5942873.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11752..13038
                     /locus_tag="OO332_002429"
     CDS             11752..13038
                     /locus_tag="OO332_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5942874.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13093..13686)
                     /gene="grpE"
                     /locus_tag="OO332_002430"
     CDS             complement(13093..13686)
                     /gene="grpE"
                     /locus_tag="OO332_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5942875.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13809..14687
                     /gene="nadK"
                     /locus_tag="OO332_002431"
     CDS             13809..14687
                     /gene="nadK"
                     /locus_tag="OO332_002431"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5942876.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14773..16434
                     /gene="recN"
                     /locus_tag="OO332_002432"
     CDS             14773..16434
                     /gene="recN"
                     /locus_tag="OO332_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5942877.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16583..16924
                     /gene="bamE"
                     /locus_tag="OO332_002433"
     CDS             16583..16924
                     /gene="bamE"
                     /locus_tag="OO332_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5942878.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16986..17276)
                     /locus_tag="OO332_002434"
     CDS             complement(16986..17276)
                     /locus_tag="OO332_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5942879.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17266..17703)
                     /gene="ratA"
                     /locus_tag="OO332_002435"
     CDS             complement(17266..17703)
                     /gene="ratA"
                     /locus_tag="OO332_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5942880.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17874..18356
                     /gene="smpB"
                     /locus_tag="OO332_002436"
     CDS             17874..18356
                     /gene="smpB"
                     /locus_tag="OO332_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5942881.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18571..18933
                     /gene="ssrA"
                     /locus_tag="OO332_002437"
     tmRNA           18571..18933
                     /gene="ssrA"
                     /locus_tag="OO332_002437"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19138..20379
                     /locus_tag="OO332_002438"
     CDS             19138..20379
                     /locus_tag="OO332_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5942882.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5304 a         5370 c         4823 g         5053 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc accatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcca atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggcg ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc agaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aagtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcacctcgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcagtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttgccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcattaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatctgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgatc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atatataaat ttacacccct ttgaaaacaa tccgctatgc tttgaaaaag
     4441 gagaaagaaa tgatgaaaaa gtttatcgcc cccttgttgg ctttactggt tagcggatgt
     4501 cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta tgatgtcggt
     4561 atggaagatg cgatatcgga cagcgccata aaagatgacg atgcatttag cgattcacag
     4621 gcggatcgcg gtctatacct taaaggatat gccgaagggc aaaagaaaac ttgccagacc
     4681 gattttactt atgcccgggg actttccggt aaaagctttc ctgccagctg caacaatgtt
     4741 gaaaatgcca gccaactgca tgaagtatgg caaaaagggg ctgatgaaaa tgccagcaca
     4801 atacgattaa attaattaaa accccacaaa taaattaaac ataagatttt actcattgtg
     4861 ttttatttct cacattgatg acggtcgcat gaaaaaatga tattttaatg agcatcctga
     4921 aaaaatatcg atgcatttcg agcgaagatg gtggggatcc ctgaatgcgt ttttctcacc
     4981 gactgttctt acttctgatt ctcctgctga cgggagcacc aatactcgct caagagcctt
     5041 ctgacgttgc gaaaaacgtg cgtatgatgg tttccggtat tgtcagttac acccgctggc
     5101 ctgcgctatc agggccacca aagttatgta tcttttcatc ttcacgtttt agtaccgccc
     5161 tacaagagaa tgccgcgaca tctttgcctt atctgcctgt catcattcac actcaacaag
     5221 aggcgatgat ttcaggctgc aatggttttt attttggcaa cgagtcaccg acattccaga
     5281 tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccgaa caaaataccg
     5341 agtgcattat tggtagcgcc ttttgtctga tcatccacaa taatgacgtc agatttgccg
     5401 taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc ttaatgctcg
     5461 cacggaagaa aaatgatgga taacgataat tctcttaata agcgccccac gtttaaaaga
     5521 gcattacgca acatcagtat gaccagcata tttatcacta tgatgttgat ctggttgctg
     5581 ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct ggcactgaca
     5641 gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg ccctgcagca
     5701 actgaaacac tggcagcgct gggccagcaa gggcattttc aactgcagaa gtacgtgata
     5761 agcagcaaaa tattctggca tcctggcatt acccccgtaa ggatccaggc gatactttca
     5821 gcaatttcat aagccactgg ctcttccctg cccccatcat tcagccgatt cgtcacaatg
     5881 gtgaaaccat tggcgaagta cgcttaaccg ctcgcgacag ttcaattagc cattttatct
     5941 ggttttcact cgccgtactg accggttgta ttctgctggc atcaggcatc gcaattaccc
     6001 tcacccgcca tttgcacaat ggcctggtgg aagcactgaa aaatatcacc gatgtcgtac
     6061 atgatgtgcg ttccaaccgc aatttttccc gacgagtttc ggaagaacgt atcgctgagt
     6121 ttcaccgctt cgctctcgac ttcaacagtc tgctggatga aatggaagag tggcagcttc
     6181 gtttacaggc caaaaatgcg cagcttctgc gtaccgcgct acatgaccca ttaaccgggc
     6241 tggctaaccg cgcagcgttt cgtagcggca tcaatacgtt gatgaacaat tccgatgccc
     6301 gaaaaacgtc ggcgttacta tttcttgatg gcgataattt caaatatatc aatgatacct
     6361 ggggtcatgc gacgggcgat agggtcttga ttgaaatcgc aaaacggtta gctgaatttg
     6421 gcgggctgcg acataaagca taccgcctgg gcggcgatga attcgctatg gtgctctatg
     6481 atgtacagtc ggaatctgaa gtgcagcaga tatgctcagc actgacacaa atctttaatc
     6541 tcccgtttga tcttcataat ggccatcaga ccaccatgac attaagcatt ggttacgcga
     6601 tgaccattga gcacgcctct gcggaaaaat tacaagagct tgccgatcac aatatgtatc
     6661 aggccaaaca ccagcgtgcc gaaaagctgg tgagataaca aggatatatc gatatgataa
     6721 agcacctggt agcacccctg attttcacct cactaatact gactggctgc cagtcccctc
     6781 agggaaagtt tactcctgag caagtcgccg ctatgcaatc ttatggattt actgaatccg
     6841 ccggcgactg gtcgctgggc ttatcagatg ccattctgtt cgcaaaaaat gactacaaat
     6901 tgctcccgga aagccagcaa cagctccaaa ccatggcagc caaattggcc tcgacagggc
     6961 taacacatgc ccgtatggat ggacacaccg ataactatgg tgaagacagt tacaacgaag
     7021 gcttatcatt gaaacgggcg aatgtcgtgg ccgatgcatg ggctattggt ggacaaattc
     7081 cacgcagcaa tctcaccaca cagggtttag gaaaaaaata tcccatagcc agtaacaaga
     7141 ccgcccaggg ccgcgccgag aaccgccgcg tcgcagtggt gattacaacc ccttaaaaca
     7201 cgagcaaaca gacaaaaaaa gggccagcca attgcccagc ccttcttaac aggatgtcgc
     7261 ttaagcgaaa tcttagttaa gacgctcttt gatacgagca gccttaccag tacgctcacg
     7321 caggtagtac agtttagctt tacgaacagc accacgacgt ttgacagaaa tgctgtcaac
     7381 taccggagag tgagtctgga agacacgctc aacgccttcg ccgttggaaa ttttacgaac
     7441 agtgaatgca gagtgcagac cgcggttacg aatagcgata accacgccct cgaatgcctg
     7501 cagacgtttt ttggaacctt caacaaccca tactttcact tccacggtat cacccggacg
     7561 gaaggaaggt acgtcctgct tcatctgctc ttgttcaagt tgcttaataa tgttgctcat
     7621 aatttaatct cttatcctgg gtaaactgat ctctcggggg cttacgccat cccatcatgt
     7681 ttatgttgct gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc ttgctcttca
     7741 gtcagagcca ggttttccag aagttcaggt cttctaagcc aggtacggcc cagcgactgt
     7801 ttcaaacgcc agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg cggaacttcc
     7861 atcccttcta acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc ttcagcaaag
     7921 gaatcttccg ttgccgaggc ttcatgtccc agtactcccg gaataaaccg ggaaacggag
     7981 tcaatcagcg tcattgctgg taactcacca ccactgagaa cgtaatcgcc gattgaccat
     8041 tcttcgtcaa tttcggtttg gatcacgcgc tcatctatac cttcgtagcg accgcacacc
     8101 agaatcaatt tttgattcgt tgccagttcg ctgacgcccg cttgatcaag cttgcgtccc
     8161 tgtggtgaca gataaatcac ctttgcgcct tcacccgccg cggcttttgc tgcatgaatg
     8221 gcgtcccgca agggttgcac catcattaac atccccggtc cgccgccgta aggacgatcg
     8281 tccacggtac ggtgccggtc atgcgtgaag tcgcgaggac tccaactctg gatgctcagc
     8341 aggccatttt taactgcccg gccagttacc ccgtaatcgg taattgcgcg gaacatttca
     8401 ggaaacaggc taattatgcc aatccacata gcgccgtctt ttaccgttta tccggtggtt
     8461 taaaaaccag gatcccaatc tacttcgatt gaacgagtag tgagatcgac tttcttgata
     8521 acctgcccat cgaggaacgg tacgagacgt tccttgatac caaacgcatc tttcaggttt
     8581 gccttaatga cgagaacgtc attagatccg gtttccatca tatcgacgac tttaccgaga
     8641 tcgtagcctt cagtggttac tacctggcag cccatcaggt ctttccagta gtagtccccc
     8701 tcttcaagct gaggcagctg cgatgaatcc acgacaattt cacaattcgt cagcaggttc
     8761 gccgcatcac gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt gtggtgcttc
     8821 cagctttcca gctggacttg ctgccactga cccgccttct ggataaacca gggctgatag
     8881 tcaaaaatgc tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc acgaataccg
     8941 taagacgaac ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc ggtgagttgt
     9001 ttgctcatca tgaccaccgt gacagattaa gctgctttgt ttacttcttt gatcagcgca
     9061 gcaacgcgat cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg atccagatcc
     9121 aggcgagtgc cttcttcttt ttcgctagcg attgggttga agaaaccaac gcgctcgatg
     9181 aagcgaccgt tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa cggacgcttt
     9241 ttagcgccgt gacgtgctaa acgaatagtt accataacat cctcttgtgt gaataaaaca
     9301 accggacccc atcgaggaac ggagtccggt gtcatattaa aagcccgaaa attttactga
     9361 tttctggaga aaatgcaatc agcagttaat aactctgtcg cataaggtca tttagcgacc
     9421 aggaaatccc ggcggcatca ttcccttcat gcttctcatc atcttcgcca ttccgccttt
     9481 cttcattttc ttcatcatgc gctgcatgtc gtcgaactgc ttcagaaggc ggttaacgtc
     9541 ctgtacctgc atcccgcaac cggcggcgat acgacgctta cgcgaacctt tgatgatttc
     9601 tggcttagcg cgctctttca tcgtcattga gttgatgatg gcttccatac gcaccagcac
     9661 tttatcgtcc atctgcgact tgacgttatc cgggatctgc cccatgcccg gcagtttgcc
     9721 catcaagctg gccatgccgc ccatattttt catctggcgc agttgctcaa gaaagtcgtt
     9781 gagatcgaag ccgtcgcctt tttttagctt gctggctaat ttctctgcct gcgcgcggtc
     9841 aactttgctt tcgatatctt cgatcagcga cagtacgtcg cccatgccga gaatacgcga
     9901 cgcgatgcgg tccggatgga acggctccag cgcctcagtt ttctcgccaa caccgaggaa
     9961 cttgatcggt ttgccagtga tgtgacgaat agagagcgcc gcaccgccgc gggcatcgcc
    10021 gtccactttg gtcaacacta cgccggtaag cggtaacgct tcattgaatg cttttgccgt
    10081 attggccgca tcctgaccgg tcatggcgtc aaccacaaac agggtttcaa ccgggttaat
    10141 cgacgcatgg acttgtttga tctcgtccat catcgcttcg tcaacgtgca gacgaccagc
    10201 ggtatccacc agcagcacgt cgtagaattt cagtttggct tctttcagcg ccgcgttaac
    10261 aatatcaacc ggcttctgac caacatcaga agggaagaaa tcaacgccca cctgctctgc
    10321 cagcgtctca agctgtttga ttgccgccgg gcgatagacg tcagcagaaa ccaccagcac
    10381 tttcttcttg tgcttctcgc gcaggaaatt accgagttta ccaacgctgg ttgttttacc
    10441 ggcaccttgc aggcctgcca tcagcacgac cgcaggcggt tgcgcagcca gattcagggt
    10501 ctggttctct tcgcccatcg ccgcaaccag ttcgttacgg acgattttga cgaactcctg
    10561 ccccggcgtc aggctcttat taacttcatg accaaccgct ttctctttta cgcgattgat
    10621 aaactcacgc actaccggca gagcgacgtc cgcctccagc agcgccatgc gcacttcgcg
    10681 cagcgtatct tttacgttgt cttcagtgag gcgtccacgg ccactgatat tgcgcagcgt
    10741 gcgcgacaaa cgatcggtta aattatcaaa cattgtctct cgcctggggt ggaaacggtt
    10801 ggccgcaatc gcgacacatc atcagtattt tgccgcagta taacatgaag gcgtctttgt
    10861 tgttatgcaa cggttggagc agcgttcacc tgacgctata ctgcttctct ttcttattgc
    10921 tcaaactgtc gacattacta tgcccgtttt tgctctgctc gcgcttgtcg cctactccgt
    10981 cagtcttgcg ctgattgttc ccggtctgct gcaaaaaaac ggcggctggc ggcgcatggc
    11041 tattatttct gcggtcattg cgctggtctg ccacgcaatc gctctggaag cccgcatcct
    11101 gcccgacggt gatagcggac aaaacctcag cctgctgaac gttggttcat tggtcagttt
    11161 ggtgatctgt acggtaatga ccattgtggc ttctcgcaat cgtggctggt tgctgctacc
    11221 cattgtctat gcctttgcgc ttatcaacct ggcgctggcg accttcatgc ccaatgaata
    11281 catcacccat ctggaagcaa cgcctgggat gctggtgcac attggcttat cgctcttttc
    11341 ctatgccacg ctaattatcg ccgccctgta cgcgctgcaa ctggcgtgga ttgattacca
    11401 actgaagaac aagaagctgg catttaacca ggaaatgccg ccattgatga gtatcgagcg
    11461 taaaatgttc cacatcacgc agattggcgt ggtgctgcta acgctcacgc tttgcactgg
    11521 cctgttctac atgcacaacc tatttagcat ggaaaatatc gacaaggctg tgctctctat
    11581 cgtggcgtgg tttgtctata ttgtgctgct gtggggacat taccatgaag gatggcgtgg
    11641 acgccgcgtc gtctggttta acgttgcggg cgcggtaatt ctgacactgg cctacttcgg
    11701 cagccgaatt gtccagcagt taatcagcta aacccagaaa aggagtttcc cctggaacac
    11761 atttctacta ctacgttgat cattattctg atcatcatgg tggtcatttc agcctatttt
    11821 tccgggtccg aaaccggaat gatgaccctc aaccgctatc gtcttcgaca tatggcgaaa
    11881 cagggtaatc gctcggccaa acgcgtcgaa aaattgctgc gtaagccaga ccgcctgata
    11941 agcctggtgt taatcggcaa taacctggtc aatattcttg cctccgcgct cggcactatt
    12001 gtcgggatgc gtttgtacgg cgatgcgggc gtggcaattg cgactggtgt gctgactttt
    12061 gtcgtactgg tttttgctga ggtattgccg aaaaccattg ccgcgctgta cccggaaaaa
    12121 gtcgcttatc cgagtagttt tctgctggct ccgctgcaaa ttttgatgat gccgctggtc
    12181 tggttgctga atgctatcac ccgtatgctg atgcgcatga tgggtatcaa aaccgatatc
    12241 gtggttagcg gctctttgag caaagaagag ttgcgcacta tcgtgcatga atcgcgctca
    12301 caaatttccc gtcgcaatca ggatatgctg ctgtcggtgc tcgatctgga aaaaatgacc
    12361 gttgatgaca tcatggtgcc gcgcagtgaa attatcggta ttgatatcaa cgatgactgg
    12421 aaatctattc tgcgccaact ctcccactca cctcacgggc gcatcgtgct ttaccgtgat
    12481 tcgctggacg acgccatcag tatgctgcga gtacgtgaag cctggcggtt gatgtcggag
    12541 aaaaaagagt tcaccaaaga aaccatgctg cgcgccgcgg acgagatcta ttttgtgcct
    12601 gaaggtacgc cgctcagcac gcagttggta aagtttcagc gcaacaaaaa gaaagtcggc
    12661 ctggtcgtca acgagtatgg agacattcag gggctggtga cggttgaaga tattctggaa
    12721 gagattgtcg gcgatttcac tacgtcgatg tcgccaacac ttgccgaaga ggtcacgccg
    12781 caaaacgacg gttcggtgat tatcgatggc accgccaacg tgcgggaaat caacaaagcc
    12841 tttaactggc atctaccgga agatgatgcc cgcacggtta atggcgtcat tcttgaggca
    12901 ctggaagaga tccctgtcgc aggcacccgc gtgcgtattg gcgagtacga tatcgatatt
    12961 ctcgacgtac aggacaatat gattaagcag gtaaaagttt ttcctgtgaa accgctgcgc
    13021 gagagtgtgg cggagtaacg aaaacggccc tgcattcgca tgcagggccg tgaattatta
    13081 cgaaaacaga aattacgctt ttgctttcgc tacagttacc atcgccgcac gaatcgtacg
    13141 accattcagc gtataaccct tctgcataat gcccagtacg ttacctggcg caacgtcatc
    13201 agattccacc attgcgatag cctgatgcac attcgggtcc agcggcacgt tagtttcggc
    13261 gatcacttca acaccaaact tacgcacaac atccagcatc gacttcagcg tcagctcaat
    13321 gccttcaacc atcgcagaca tatccgggtt agctttatca gccacttcca gcgcacgatc
    13381 caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga atttgtgggc
    13441 tttttcaata tccagttcag tacgacgacg caggttttcc atttcggctt ttacgcgcaa
    13501 aataccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat tcgcaatttt
    13561 ttcatcgcgc ggatccacct gctcagcaga agcttctggc tcaactgcct caatctcttc
    13621 gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct gttctttact
    13681 actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct tattatgggg
    13741 atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc aacaaggacc
    13801 tcggaaaaat gaataatcat ttcaagtgca ttggcattgt gggacaccca cggcacccta
    13861 ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt tacgaggtca
    13921 tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact ggcacgctcg
    13981 cggagattgg gcaactggct gatctcgcgg tagtcgttgg tggcgacggt aatatgctgg
    14041 gcgcggcgcg cacgctcgcc cgttacgata ttaaagttat tggaatcaac cgtggcaacc
    14101 tgggtttcct gactgacctt gaccccgata acgcccagca acaattagcc gatgtgctgg
    14161 aaggtcacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt cagcaagatt
    14221 gccaaaaacg catcagcacc gcgattaacg aagtggtact tcatcctggc aaagtggcgc
    14281 atatgataga gttcgaagtg tatatcgacg agatctttgc gttttctcag cgatctgatg
    14341 ggctaattat ttcgacgcca acaggctcca ccgcctattc cctctctgcg ggcggtccta
    14401 ttctgacccc ctctctggat gcgattaccc tggtgcccat gttcccgcat acgttgtcag
    14461 cacgaccact ggtcataaac agcagcagca cgatccgtct gcgtttttcg catcgccgta
    14521 acgacctgga aatcagttgc gacagccaga tagcactgcc gattcaggaa ggtgaagatg
    14581 tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat tacagttatt
    14641 tcaatacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt tacgccagcc
    14701 tctttactgt atataaaacc agtttatact gtacacaata acagtaatgg tttttcatac
    14761 aggaaaacga ctatgttggc acaactgacc atcagcaact ttgctatcgt tcgtgagctt
    14821 gagattgatt ttcatagcgg catgaccgta ataactggcg agaccggtgc gggtaaatct
    14881 attgcaatag atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga catggtgcgt
    14941 accggcgctg ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac gccagcggcc
    15001 ctgcgctggc tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct tcgtcgcgtg
    15061 atcagcagtg atggtcgctc ccgtggtttc atcaacggta cagctgttcc tctgtcacaa
    15121 ctgcgcgaac tgggtcagtt gctgattcag atccatggtc agcacgctca tcaattactc
    15181 accaaacctg agcaccaaaa attcctgctt gatggctatg ccaatgaaac ctctctactt
    15241 caggaaatga ccgcacgtta tcagttgtgg catcaaagct gccgtgacct cgcgcatcat
    15301 caacagttaa gtcaggaacg cgccgcccgt gcggaactgc tgcaatacca attaaaagaa
    15361 cttaacgaat ttaatccgca gcccggagag tttgagcaaa tcgacgaaga gtacaaacgt
    15421 ctggcgaaca gcggtcaatt gctgaccacc agccagaatg cattggcatt aatggccgac
    15481 ggtgaagacg caaacctgca aagtcagctt tacacggcta aacaactggt gagcgaattg
    15541 attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaagc taccatccag
    15601 attgctgaag ccagcgacga actgcgccac tactgcgatc gtctggatct cgatcccaac
    15661 cgactgtttg aacttgaaca gcgcatctca aaacagattt cgctggcacg taaacatcac
    15721 gtcagccctg aggcattacc acagtattac cagtcgctac tggaagaaca gcagcaactg
    15781 gacgatcagg ccgactcaca agaaacgctc gcgctggcgg taacgaaaca tcatcagcag
    15841 gcactggaaa ccgcgcgcgc attacaccaa caacgccagc aatatgcaga agaacttgca
    15901 cagctgatca ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt tacgattgat
    15961 gtcaaatatg acgagcatca cctgggcgct gacggtgccg atcgtattga gtttcgggta
    16021 accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg tggtgaattg
    16081 tcccgcatcg cactggcaat ccaggtcatc acggcgcgta aaatggaaac cccggcactg
    16141 atttttgatg aagtggatgt agggattagc ggtccaacag cggcagttgt cggcaaactg
    16201 ctgcgtcaac tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc acaagtcgcg
    16261 ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat gacagaaaca
    16321 catatgcaat ctctggataa aaaagcgcgg ttacaagagc tggcgcgcct gcttggtggc
    16381 agtgaagtca cacgtaacac actggcgaat gcgaaagaac tgcttgcagc gtaaactttt
    16441 ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag caaaaggttt
    16501 taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt actgctcggg
    16561 cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag cagcagtact
    16621 attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc ctgacatcaa
    16681 ccaggggaac tatctgaccg ctaacgacgt atccaaaata cgtgttggca tgacgcaaca
    16741 acaagttgcg tacgcattgg gtacaccgct gatgtccgat ccatttggta cgaatacctg
    16801 gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa cgctgacgct
    16861 gacctttaac agtagcggtg tgttgaccaa tattgataac aaacctgcgc tgagtggtaa
    16921 ctaataataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca ccttttttct
    16981 gtctgttatt tattcgctga tttttctgct cgttgcctgc gaagctcttt cggatcggca
    17041 atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct tagttttgcc
    17101 ggacggctgt aaatgccgac tttgttttta gttaaatcga tatcggtacg caattccagc
    17161 aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt cactcgctgc
    17221 aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc tagcactgta
    17281 aacctctttc gcacgaaccg taaaagcctg gaccatatta gccgccagct ctttaaacac
    17341 gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca aagtcgagat gaaactcgat
    17401 acgacacgcc tcctggctca gcggcgtaaa cttccatcca ccaatcaatt tcttgaacgg
    17461 cccatccacc agattcataa gaatactttg gttactggtc aactggttgc gggtagtaaa
    17521 cgttttgctg atcccagcct tagagacatc taccgccgca gtcatctgcc caggagtgga
    17581 ctccagaatc cgacttccgg tacaacctgg caaaaactga ggataagact gaacgtcatt
    17641 cactaactga tacatttgct ccgcgctgta gggtaccagt gcggtccggc taatctgagg
    17701 cataacaatt tccatcaaca aaaatccaac aaataatatc atttatcctg ctgtaaaaaa
    17761 aacgctatcc cggcgctggg taacatcggg ttcatgctaa gatagagcct tgtcccccgc
    17821 aggattgata tggggtgttt tcgatttcag attaccgatg attcacgacg cttatgacga
    17881 agaaaaaagc acataaacct ggttcagcga ccatcgcgct taacaagcgc gcccgtcacg
    17941 aatactttat cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg gaagttaaat
    18001 ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt ccttctgcgt gacggagagg
    18061 catttctgtt tggcgctaac atcacgccaa tggccgtggc ctccacgcat gtggtgtgcg
    18121 atcctacccg tacccgcaag ttacttctca accagcgcga actggactca ttgtacggtc
    18181 gcgtcaatcg agaaggctat accgtagtgg cgctctccct gtactggaaa aatgcctggt
    18241 gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca cgataaacgt tcagatatca
    18301 aagaacgcga atggcaggtg gataaagcac gcatcatgaa aaacgcccac cgttaaacct
    18361 gcactccaat tattgaccag ttcctcaccg cgcctccctc tccggcggcg cgaatgaaca
    18421 tcttattggc tatcacatcc gacacaaatg ttgccatccc attgcttaat cgaataaaaa
    18481 tcaggctaca tgggtgctaa atctttaacg ataacgccat tgaggctggt catggcgctc
    18541 ataaatctgg tatacttacc tttacacatt ggggctgatt ctggattcga cgggatttgc
    18601 gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa aaaatagtcg
    18661 caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct ctctccctag
    18721 cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg cgtggaagcc
    18781 ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc gtgtccgtcc
    18841 gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggat gtaggaattt
    18901 cggacgcggg ttcaactccc gccagctcca ccaaaattct ccatcggtga ttaccagagt
    18961 catccgatga agtcctaaga gcccgcacgg cgcaagccct gcgggctttt ttgtgccccg
    19021 aatttgtccc gcgaagtctg atgccaacta attaaatccg atccttttag gcaccttgtt
    19081 aggcacttca taaagctata ttgtctttga ggtgcctaaa actatggaaa cccggcaatg
    19141 gcaagacaaa ccaaacctct atccgttaag gaaatcgaat ctgctaaacc caaggaagcg
    19201 gactacgttc tctatgatgg cgatggcctt gagctactca ttaaatccag cgggagtaaa
    19261 atctggcagt ttcgctacat tcgccctgtc accaagacac gagcaaagaa aagcataggc
    19321 ccttacccgt cagttaccct tgccgatgcc agaaactacc gtgcagagtc ccgctcactc
    19381 ctagctaaac agattgagcc gcaggaacat cagcaagaac aattgcgtag ttcgctggaa
    19441 gccaaaacca atactttcca gctcgtagct gaacgttggt ggaatgtgaa gaaagccagt
    19501 gtgacagagg actatgcgga agatatctgg cgctctcttg aaagagatgt ctttcctgcg
    19561 attggcgacg ttagcgttac agatattaaa gctcatacac tggttcaggc cgtacaaccg
    19621 gttcaggcca gaggagcact ggaaacggtt cgtcgccagt gtcaacgtat taatgaggtc
    19681 atgatctatg cccagaacac agggctgatt gatgctgttc ccagcgttaa tatcggtaaa
    19741 gccttcgaaa agcctcagaa aaagaacatg cccagcattc gaccggatca gctatctcaa
    19801 ctgatgcaga caatgcgaac agccagcatt agcctttcca cacgctgcct gttcatgtgg
    19861 caacttctta ctattacccg ccctgccgaa gcggctgaag ctcgctggga agaagtagac
    19921 atagaagcgc aagagtggaa gattcccgca gcacgcatga aaatgaaccg cgaccatact
    19981 gttccattgt cagatgaagc aatagctgtg ctggagatga tgaaaccgct aagcggcaac
    20041 cgagaattta tctttcctag ccgtatcaag cccaaccagc ctatgaacag tcagactgtt
    20101 aacgcctcac taaaacgcgc aggttttggt ggtgtgcttg tttcgcatgg tctgagatca
    20161 attgccagta cggctcttaa tgaacaaggc tttccgcctg atgccattga ggcagcattg
    20221 gcccatgtgg ataagaatga ggttcgtcgt gcttataacc gcagcgatta cctggaacag
    20281 cgtcgcccga tgatgcaatg gtgggcaaat tttgtgatgg cagcggatcg tggaagcatg
    20341 attgaaggtg ggataaaagg aatgaagcta gttgggtgac taaatagctg cgcggcatag
    20401 tgtcccactt tcagccagcc cttgcaggtt aagccaccag acagtaaacg gcaggtgtcg
    20461 cgttattagg cgcagctatt tagtagtaat attaggattg gtgattctgc actccagaag
    20521 ttcggtaatg ctgccaactt actgatttag
//
LOCUS       DAJTRQ010000066        20532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000066 DAJTRQ010000000
VERSION     DAJTRQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(126..554)
                     /gene="mxiM"
                     /locus_tag="OO332_002439"
     CDS             complement(126..554)
                     /gene="mxiM"
                     /locus_tag="OO332_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5942883.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(538..945)
                     /gene="mxiM"
                     /locus_tag="OO332_002440"
     CDS             complement(538..945)
                     /gene="mxiM"
                     /locus_tag="OO332_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5942884.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(938..1633)
                     /gene="mxiN"
                     /locus_tag="OO332_002441"
     CDS             complement(938..1633)
                     /gene="mxiN"
                     /locus_tag="OO332_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5942885.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(1575..2102)
                     /locus_tag="OO332_002442"
     CDS             complement(1575..2102)
                     /locus_tag="OO332_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5942886.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(2099..2824)
                     /gene="mxiJ"
                     /locus_tag="OO332_002443"
     CDS             complement(2099..2824)
                     /gene="mxiJ"
                     /locus_tag="OO332_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5942887.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(2830..3123)
                     /gene="mxiI"
                     /locus_tag="OO332_002444"
     CDS             complement(2830..3123)
                     /gene="mxiI"
                     /locus_tag="OO332_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5942888.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(3136..3387)
                     /gene="mxiH"
                     /locus_tag="OO332_002445"
     CDS             complement(3136..3387)
                     /gene="mxiH"
                     /locus_tag="OO332_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5942889.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(3395..4510)
                     /gene="mxiG"
                     /locus_tag="OO332_002446"
     CDS             complement(3395..4510)
                     /gene="mxiG"
                     /locus_tag="OO332_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5942890.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(4519..4977)
                     /gene="ipgF"
                     /locus_tag="OO332_002447"
     CDS             complement(4519..4977)
                     /gene="ipgF"
                     /locus_tag="OO332_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5942891.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(4977..5339)
                     /gene="ipgE"
                     /locus_tag="OO332_002448"
     CDS             complement(4977..5339)
                     /gene="ipgE"
                     /locus_tag="OO332_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5942892.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(5349..6965)
                     /locus_tag="OO332_002449"
     CDS             complement(5349..6965)
                     /locus_tag="OO332_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5942893.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            7279..8763
                     /gene="icsB"
                     /locus_tag="OO332_002450"
     CDS             7279..8763
                     /gene="icsB"
                     /locus_tag="OO332_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5942894.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            8776..9165
                     /gene="ipgA"
                     /locus_tag="OO332_002451"
     CDS             8776..9165
                     /gene="ipgA"
                     /locus_tag="OO332_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5942895.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            9181..9807
                     /gene="ipgB1"
                     /locus_tag="OO332_002452"
     CDS             9181..9807
                     /gene="ipgB1"
                     /locus_tag="OO332_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5942896.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            9864..10331
                     /gene="ipgC"
                     /locus_tag="OO332_002453"
     CDS             9864..10331
                     /gene="ipgC"
                     /locus_tag="OO332_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5942897.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            10337..12079
                     /gene="ipaB"
                     /locus_tag="OO332_002454"
     CDS             10337..12079
                     /gene="ipaB"
                     /locus_tag="OO332_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5942898.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            12099..13190
                     /gene="ipaC"
                     /locus_tag="OO332_002455"
     CDS             12099..13190
                     /gene="ipaC"
                     /locus_tag="OO332_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5942899.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            13241..14239
                     /gene="ipaD"
                     /locus_tag="OO332_002456"
     CDS             13241..14239
                     /gene="ipaD"
                     /locus_tag="OO332_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5942900.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            14248..16149
                     /gene="ipaA"
                     /locus_tag="OO332_002457"
     CDS             14248..16149
                     /gene="ipaA"
                     /locus_tag="OO332_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5942901.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            16153..16389
                     /locus_tag="OO332_002458"
     CDS             16153..16389
                     /locus_tag="OO332_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5942902.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            16606..17535
                     /gene="virB"
                     /locus_tag="OO332_002459"
     CDS             16606..17535
                     /gene="virB"
                     /locus_tag="OO332_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5942903.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(18482..19261)
                     /gene="ipaJ"
                     /locus_tag="OO332_002460"
     CDS             complement(18482..19261)
                     /gene="ipaJ"
                     /locus_tag="OO332_002460"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5942904.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(19861..19998)
                     /locus_tag="OO332_002461"
     CDS             complement(19861..19998)
                     /locus_tag="OO332_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5942905.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT         7029 a         4169 c         3031 g         6303 t
ORIGIN      
        1 gagccagctc aattatgtta cgttctttat cttttacaag ctcaacgtta actggttcat
       61 gtccactaga gtaaatgtaa aacatattac ttgtatttag acctttatat ttactcacaa
      121 tacaattata tccctcgaat taattttaat ttaacttccc catccttctc tataaggatg
      181 gtatcattta tcttattacc ttttaaaaca accccgtctt tcagcataga aaatagttta
      241 tctatagcta tcttttgtgc atccatgaag cacccatcta cttccttaac attaaagttg
      301 cccatgaatt tacctgcatt aaaactataa accgccttac cactaataca tttagagtaa
      361 acatttatac ttttattggt tgtattaaac gaccataata aatcatttgg aatagaaaaa
      421 taatccttac ttacaggaac aatatgccat tctttttcag aattagatga tgactttaaa
      481 gcacacccac tcagtgttaa tagcaatata cttaaaataa atattttcaa cttattacta
      541 ccatgtcgaa tcatctgcct ctctccctgc ttcaaagaca ctaattaatg aatcgaaggt
      601 tgtagtctta atatagaaat caatattgtt atatatggac aatgatgcat aaaatccctc
      661 atgcaaatcg acatattgat ttgcatctat gagtttatta agaagtgaac ttttttgtgc
      721 agctgtaaga taaggaaaac gtgaattgaa taattgttca atcttattgt gcagttcatc
      781 attatctatg gaatttaatt cactctgtga gaaatattgc attatttcat agattatatc
      841 ctcatcaaat gattgcgaag atgataatct ctcatttaaa ttaacaaatt cttcactatt
      901 taatctttgc tgcagagcat tgcttgcatt tatttgatta atcattaaca ggattctctt
      961 ttaacaagtc ttctgcaatt tgtctcagtt tcttatgtgc taactctttg aaatattttt
     1021 tatcattttt tgttaatttt tccgcaagtt ctattttaac acctgatatt acatcctgag
     1081 gactaaactc cactacttga ttaccactac agaatatata attattccct tcagaatatt
     1141 ttaaaataat cttgacatct gagcgatatt tatggagatc aagttctaac tttttactca
     1201 gtgctaaatt acacttgggc aaaaccactt ttaattctgt tactgtatta cgcaacgaac
     1261 taagccactg ctccaataaa gtgctaatga tcctctcatc atgtaaattt tctgaaagca
     1321 aactagttat atagtcctca atatttcttc tgttttcatt ttgctttaac ttccattcac
     1381 aaatatagtc aataatatgc tcaaaagcag tctgtatacc aatttgataa ccatcacata
     1441 cagcatctat acgcagtgat tcagcttttt tcgtagcctc ctttataaca caaattgctt
     1501 tcctcttact ctcagtgtct ctatctttta tggttttaca taattccttt tcaattctct
     1561 ttataacaac accatcatag gcatgatgtc tggataccgg taaagtgcct ttttgcatat
     1621 tgcatacctt catttaatcg ggacaaattt ctttctacag gcgtataatt tccctccaaa
     1681 gcaattggat taaataataa gtttattctt tcatatatac caacagatat attttccttc
     1741 cacgcaataa gatgccaaaa accacttcga atagcggcct ccccttgctt actgattttg
     1801 cccccataat aactaataac catctcacta tgattaatta accgagactc ttgactaccg
     1861 ataaaataag caacagctgg aagtaaattc cagttggcaa caaacatagc ggttaccccg
     1921 cttaaaggtt caatatcgca atttagatca tatttattaa ttatgagatt attaatctca
     1981 cttctgatta cgccattttc taacagttca gaaggcaaat tcaaccgatt tctatttata
     2041 tagaacgctg gatcaaaaat tattgaaaga tattttttat aaattccatc cattcttatc
     2101 atattttgtt tctcttgaac caccctgttt tgaatgccca taccaaaaga atgacaacta
     2161 gtacagccat cccgagaaat aaatatatta cttcatttgt taaaaattcc gatttaactt
     2221 ccttaacagg ttgtacattt gtataaacat attcttcttt cggagttaat atgacagata
     2281 tattttcata cttaacatca gaaaaggtgt ttttcaaaaa tcgcttaata ttactaacta
     2341 atagttcaga ctctttcggt gagtcatata tagcgattac tgatatatgc atcggttttg
     2401 aagatatatt tttttcttca agatcatagc taacatgtat ttttgccgaa ataacaccac
     2461 caatagaaac taaagactgt tccagccgtt gctcaatagc actatataaa cgggcctttt
     2521 cagctcttgg agaagacact aatgaatctg taggaaacat ttgtgagata tcaactctct
     2581 ccggatttgg caaatcgtac atgcgcatca aatcaactgc cgatgcaaat gtcccctttt
     2641 cgacttgtac cgagatcccc tgtttacctc catcaacttt tctagcagta atattatggc
     2701 gttctagcac agatattatt tcatttgcct gtctttgaga taaattagaa attaactctt
     2761 cacgttgctc acatccaatc aacatcagca acaagaataa aataaaacct ttatacctaa
     2821 tcatataaat taagacttta ataaagtttc aacagccgaa actgtttttc ttgctaacgt
     2881 gccagctaat gatactccta ttgaataatt tgagagcgtc gtctgtaact tggccaaaga
     2941 ttctgggttt aatgattctg gattgcttac catctccatt atttgtgata cttgaatatc
     3001 tgtatccatc ttaatatcac tatatttagc cgacacgacg tcttccagac ttgatatctc
     3061 ttgagattga aaatcactgg ctttgataat atcaacctga ttgactggat aaatgtaatt
     3121 catgaatgct ccctattatc tgaagttttg aataattgca gcatcaacat ccttaatcac
     3181 tttcactgta ttggattgcg cgttcctata taatgtatat tcagataatt tactttggta
     3241 ttcagccagc aactgtggat tcgaaggatt tctagctaat ttatctagtg ccaatgttag
     3301 ttcaccttgt aatgtttgag ttccatcatc aaaagtttca gataatgaac tcaatgtcca
     3361 atcatcattc ggtactgtaa cactcatttt atcctcactt atttttatct aaaaaaaacc
     3421 agtgtttatc attcaacatt acataactgt ctttgctgtt aagatatgat ttacctttaa
     3481 aatcatcatc tatcaaaagt acagaaaact caatatactg atctccatat atttttttat
     3541 gttcagaaat cagtctatta atgagctcaa gcttttcatc tgtcagttct tctctgacag
     3601 aatatgtaac tttattattt tcacaaatct ctttatactg gacatttacc tttgtgaaaa
     3661 tacctttagc atcccccctt gcattatgat cgctaagata gttgaattta atatttttga
     3721 tatacggaaa ttcaacaatc agagcatttt tcaatttatc aagttcggta tcttttgaac
     3781 tatttctttc tttacttaaa aacaccagta attccgcttt atcactcact aaattcaaca
     3841 catacaaatt gataaatggg aatcgttgat tgatatattg ttgaattcta tttatttctt
     3901 tgtttgacac cagaatatat ctgcatttgg taaatccggt tttatttaag gctacagacg
     3961 cccataccgc atcattttgt gtttcggcaa gaatataatt gcactgtcca gtgttgacta
     4021 ttccatatct cttatcatct ataatttcag caatttcttt tacttcatct ttctttaaag
     4081 agagggaaaa aattataatt aacacaacaa taacagcaaa gaaaaaaaac accgagtggt
     4141 tcttatacat tccgtttagt atatggcctc tcgatttatc ctccctcata tttttaaggt
     4201 gaaaagaaat cccgtcatat tcaaatgaat taaattgtga attgatatca atttgctcac
     4261 ctttttcatc actaaattga cagatattat ctttgcttat ggtcaaataa attataccat
     4321 ccgatttact atctgttaca ggaataatat tttctgggaa attgtcgttt ccaaactcaa
     4381 gtgtttctat cgtccgtccg agaacaatta agttattccc ataatataac ggaaactcat
     4441 ccccgacacc gtttgtgagt ttcactaata acctgaatgg tgcaagattt gagttctttg
     4501 cctcagacat aaatcctctt atatccttcg attattctgc ttgctctctg ctgctatccg
     4561 caaatagcgt ctatagatat cctctgcgta ctttaatctt tcttttttct ttttcggaga
     4621 cgttcctgca ttataggctc caacagcctc ccaccctctt ccatacatca tcataaactc
     4681 atttaataac tttgctgcat aatgtacaga aagacatgga tgactaatta gcatttcctc
     4741 agaatatccc atttctctaa gtcttttaga atgaaagtca tttatttgca ttataccata
     4801 atctttactt ccattgttat taacattgac tgcagattta ttaaatccgg attctttttc
     4861 cgcaatcgct tttaacaaac ttgatggaat attgtacctt tcaccagcct tatcccaaca
     4921 atcagctctc cctaaatatg ggataaaaca cagcaaaata aatacgaaac gggacattaa
     4981 taccccttca ttcttcgcgc aaattcatcc ttcagccaac gcacacgcga aatataatat
     5041 tctgtattaa catatacatc ctcgaattca tttatcccgg taagatctaa tcgcgcaatt
     5101 agatttccgc cttcatcatc agtagctaag catatctttt ctgagtaatt taggctcaac
     5161 gcatatataa gatcatttat attttcaggc aatgcacaaa aagggcataa taaatttatt
     5221 gaatctcctt ctttttcaat atatattagc acatcatcat caagcatgat tgcttgatca
     5281 tcgatatcaa ttaaagggat acccaaggct cggcaaataa catctgctaa atcttccata
     5341 tattcctctt atacaaatga cgaataccct ttcaccatat tccatatttt tgggtcccct
     5401 attctttcag aataagatag ctctaacgaa gatagcggca attttttcat gactttattt
     5461 ccgggaacac cagtattcat ctcttggatt tccatattac cactattcat tagaatagta
     5521 gaaaataatc ttttctcttc tgaggataat ttactattta attgagaaaa ctgacctgtt
     5581 tcatgttttc ttattatttc tcttttaatt tcagcatcct gcatacctgt tctgtccttc
     5641 ccactcttac aattccagca aggtacagca cctatagtat aagccaatag agtcattctt
     5701 tgtggcaatt tatacggctc tccattatca tttttttgta acttcttgtt tataatctct
     5761 tttatctggt tagccagata tatcacatca ttcttacatg gtggattttt ttctattgct
     5821 tctgcagccc aacctccaat cacaccattt ttgaggaaat tatcccccaa caaagaacat
     5881 attgattcat cattaagctt gtcaacattc ctccagccta agcccatttt tagcgctaat
     5941 tcatttacac caaagttaaa tgtcaccact tttagattaa cgcttacttc tttcagaaga
     6001 ccatcactat tccgaattaa taatttcgtt ggcttccccc ttttagagtt taaccctttt
     6061 aatgcgttca cctgatcttt taacatactc tcctctcccc cggttaaact ggtcggcgtc
     6121 agaagagaag tcgaaacaat ctttaaatct actgttttac cagacaaagc ctgtgataat
     6181 aactctggtc tgctatataa tgctgcgctt actaactcct cagctttatt acgagcagca
     6241 acagctcttt cagatgagtt ttttttcaat ccataggctg atataactcc atgtctgata
     6301 cccgaaaaaa tttccttccc ctcgtcatcc accacttttg aaagccacat attcgcgata
     6361 tgctcactct ctctcgtaga agcacaacaa attccttttc cattatactc tttgacaaat
     6421 atatttttat tccctatctt catatgagag gcaggtgtta attgaaaacc ataatttttc
     6481 ccatgatgac taatattctt gtcgaccggt ccccaattct tattatttaa ataatcagta
     6541 aatatttgtt ttattttcgt ttgcaattcc tttttatttt taatgcctag ctgtgctctt
     6601 atctcttcac ttattacttt attaagctca ttaatatgtc ttttattaac ttcaccaagt
     6661 ttataggtat ctaatcgttc catcatggct ttaatttttg acgtatcacc acctgccaac
     6721 tttattgcct cgtaaaaaac ttcagctctg tcacgcgcaa acaaaaggct ttttcctgtt
     6781 aagcttgttt ggttctgtaa atataatcga ttaagcgctt cgatcccctt atttctttct
     6841 ccatttttaa ctctttgata tgaaatcacg cttacacctt tgtgctttcc ggtttcttct
     6901 gcgcctttat aggaatctcc gctttgaaat gaaacctgat gcaatcccaa attagttatg
     6961 tgcatttaat tatcctcagt cataaatggg gcattgattg cagttttgta cctcgtttta
     7021 caacaaaaaa gatacttatt caacagggtt ctctcttttt ctataacact ccgagcctga
     7081 tacatcaggc actttatctt gtgggatttc atgatgaaac gagcactaca tatgctcacg
     7141 aggtacatat tatgtacagc aaaagaaaat ttgattatgt taacaggctc aagtttctaa
     7201 atttattaat tgaatatatt atatgccaaa tacattttac atccccacaa tcaccaagta
     7261 atggagagtt aataaagtat gagcctcaaa attagcaatt tcattgacgc aagcaataca
     7321 aaagggccta tacgcgttga agatacagag catggaccta tattgatagc acagaaattc
     7381 aacctaaagg atctcttttt cagaacatta agcactatta atgcaaagat aaattcacag
     7441 atattaaatg aacaactaaa aaattatcga ctagagaatc aaaaatcctt attgcttttc
     7501 ctcaatacat tagctagcga aaaatctgca gaaagtgcgt ttgccgccta tgaagcagct
     7561 aaaaactcaa ttcaacactc tttcacaggt agagacatca aattaatgtt gaataccgca
     7621 gagcgttttc atggcatcgg tacagccaaa aatcttgaaa gacatttagt ttttcgttgc
     7681 tggggaaata gaggcataac ccatttaggg catactagta tcagtataaa aaacaattta
     7741 ctacaggaac caactcatac ctatctcagc tggtatcccg gcggggatgt tacaaaagat
     7801 acagaaataa actacctttt tgaaaaacgc ttaggttaca gtgtcgatac ctataagcaa
     7861 gacaaactaa atatgatttc agaccaaaca gccgaaagac ttgatgcagg ccaagaggtt
     7921 agaaacttat taaattcaaa acaagatcaa aataacaaca aaaaaatatt ttttcccaga
     7981 gctaatcaaa aaaaagaccc ctatggttat tggggggtca gtgctgataa agtatacatt
     8041 ccattatcag gtgataataa aacaaaggat ggtaaaatca gccataacct ttttggcctt
     8101 gatgaaacaa acatgtcaaa atttatatgc aaaaagaaag cagatgcttt cagacaattg
     8161 gcaaattata aattaatcag taaatctgaa aactgtgctg gtatggcact taatgttctt
     8221 aaagcaggaa attctgaaat atactttcca ctccctgacg ttaaattggt tgctacacct
     8281 aacgatgtat acgcgtatgc aaataaagtt aggcaacgca ttgaaagtct caatcagtct
     8341 tataacgaaa taatgaagta tattgaatct gattttgatc tttccagatt gactcaatta
     8401 cgacgcagct atcttaaaag tttcaataaa attaacctta tccacacacc aaaaacattc
     8461 aagcctttat caatttcact ttacaaacat cctactgaaa atgtatcttc agaagacttt
     8521 gatgtggtca tcaacgcttg ccactcatac ttagtaaaat cagcacccag caatatgact
     8581 cgtgtattaa acgaattaaa aaccgaagca acagataaaa aagaagaaat tatcgaaaaa
     8641 tcaataaaaa ttattgatta ttacaattct cttaaatcgc cagatcttgg aaccaaactt
     8701 tacattcacg acttattaca gatcaataag cttttattga ataactctca ttctaatata
     8761 tagaaggcca tagaaatgtg tcgcaaacta tatgataaac tttatgaaat aacaggtgca
     8821 aagcttgatt ttaacgataa aaatcaagcc tttatattgc tcgaagaaca aatacctgtt
     8881 tgtataacag ataatgatga atacatcttt ttgaccggac ttcttaatga acatgaactt
     8941 tttacagaaa atattattaa tccagaacac atactcatcc ttaactactc tttgagtaga
     9001 gattatggtt cttctatctg tcttctccct gatactcatc aatgtgtttt gaccaagaaa
     9061 cactacaaaa aatatctttc acctgatgaa ctcattgaat ctttgtatga atttttattt
     9121 tgtattaaac taaccattgc aaacatcact tcagaagtga actaacatat agggggtatc
     9181 atgcaaattc taaacaaaat acttccacag gttgaattcg cgattcctcg cccatcattt
     9241 gattcgttaa gtcgcaataa gcttgtaaag aaaatattat ctgtttttaa cttaaaacaa
     9301 cgctttcctc agaagaattt tggatgcccg gtgaatataa ataaaatcag ggacagcgtc
     9361 atagacaaaa taaaagatag taattcgggg aatcaactat tttgttggat gagccaagag
     9421 cgaacctctt atgtctcttc aatgataaac agaagtatag atgaaatggc catacacaat
     9481 ggagttgttt tgacctcaga taacaaaaaa aacatatttg cagctattga aaaaaaattc
     9541 ccggatataa aacttgatga aaaatcagca caaacatcta tcagtcatac agcactaaac
     9601 gagattgcct catctggcct cagagcaaag attttaaaac gctatagcag tgacatggat
     9661 ttgtttaaca cacaaatgaa ggatcttaca aatctagtat ccagttccgt ctacgataaa
     9721 atatttaatg aatcaacaaa agttttacaa atagaaataa gcgcagaggt tctaaaggct
     9781 gtataccgtc aaagcaatac aaattaaatc ttttatatta aatcttatac ttattaaagt
     9841 aaagcagaca aaaaggagac cttatgtctt taaatatcac tgaaaatgaa agcatctcta
     9901 ctgcagtaat tgatgcaatt aactctggcg ctacactgaa agatattaat gcaattcctg
     9961 atgatatgat ggatgacatt tattcatatg cttatgactt ttacaacaaa ggaagaatag
    10021 aggaagctga agttttcttc agatttttat gtatatacga cttttacaat gtagactaca
    10081 ttatgggact cgcagctatt tatcagataa aagaacagtt ccaacaagca gcagaccttt
    10141 atgctgtcgc ttttgcatta ggaaaaaatg actatacacc agtattccat actggacaat
    10201 gccagcttcg gttgaaagcc cccttaaaag ctaaagagtg cttcgaactc gtaattcaac
    10261 acagcaatga tgaaaaatta aaaataaaag cacaatcata cttggacgca attcaggata
    10321 tcaaggagta attattatgc ataatgtaag caccacaacc actggttttc ctcttgccaa
    10381 aatattggct tccactgagc ttggagacaa tactatccaa gctgcaaatg atgcagctaa
    10441 taaattattt tctcttacaa ttgctgatct tactgctaac caaaatatta ataaaactaa
    10501 tgcacactca acttcaaata tattaatccc tgaacttaaa gcaccaaagt cattaaatgc
    10561 aagttcccaa ctaacgcttt taattggaaa ccttattcaa atactcggtg aaaaatcttt
    10621 aactgcatta acaaataaaa ttactgcttg gaagtcccag caacaggcaa gacagcaaaa
    10681 aaacctagaa ttctccgata aaattaacac tcttctatct gaaactgaag gactaaccag
    10741 agactatgaa aaacaaatta ataaactaaa aaacgcagat tctaaaataa aagacctaga
    10801 aaataaaatt aaccaaattc aaacaagatt atccgaactc gacccagagt caccagaaaa
    10861 gaaaaaatta agccgggaag aaatacaact cactatcaaa aaagacgcag cagttaaaga
    10921 caggacattg attgagcaga aaaccctgtc aattcatagc aaacttacag ataaatcaat
    10981 gcaactcgaa aaagaaatag actctttttc tgcattttca aacacagcat ctgctgaaca
    11041 gctatcaacc cagcagaaat cattaaccgg acttgccagt gttactcaat tgatggcaac
    11101 ctttattcaa ctagttggaa aaaataatga agaatcttta aaaaatgatc tggctctatt
    11161 ccagtctctc caagaatcaa gaaaaactga aatggagaga aaatctgatg agtatgctgc
    11221 tgaagtacgt aaagcagaag aactcaacag agtaatgggt tgtgttggga aaatacttgg
    11281 ggcactttta actatcgtta gtgttgttgc agcagctttt tctggaggag cctctctagc
    11341 actggcagct gttggtttag ctcttatggt tacggatgct atagtacaag cagcgaccgg
    11401 caattccttc atggaacaag ccctgaatcc gatcatgaaa gcagtcattg aacccttaat
    11461 caaactcctt tcagatgcat ttacaaaaat gctcgaaggc ttgggcgtcg actcgaaaaa
    11521 agccaaaatg attggctcta ttctgggggc aatcgcaggc gctcttgtcc tagttgcagc
    11581 agtcgttctc gtagccactg ttggtaaaca ggcagcagca aaacttgcag agaatattgg
    11641 caaaataata ggtaaaaccc tcacagacct tataccaaag tttctcaaga atttttcttc
    11701 tcaactggac gatttaatca ctaatgctgt tgccagatta aataaatttc ttggtgcagc
    11761 gggtgatgaa gtaatatcca aacaaattat ttccacccat ttaaaccaag cagttttatt
    11821 aggagaaagt gttaactctg ccacacaagc gggaggaagt gtcgcttctg ctgttttcca
    11881 gaacagcgcg tcgacaaatc tagcagacct gacattatcg aaatatcaag ttgaacaact
    11941 gtcaaaatat atcagtgaag caatagaaaa attcggccaa ttgcaggaag taattgcaga
    12001 tctattagcc tcaatgtcca actctcaggc taatagaact gatgttgcaa aagcaatttt
    12061 gcaacaaact actgcttgat acaaataagg agaatgttat ggaaattcaa aacacaaaac
    12121 caacccagat tttatataca gatatatcca caaaacaaac tcaaagttct tccgaaacac
    12181 aaaaatcaca aaattatcag cagattgcag cgcatattcc acttaatgtc ggtaaaaatc
    12241 ccgtattaac aaccacatta aatgatgatc aacttttaaa gttatcagag caggttcagc
    12301 atgattcaga aatcattgct cgccttactg acaaaaagat gaaagatctt tcagagatga
    12361 gtcacaccct tactccagag aacactctgg atatttccag tctttcttct aatgctgttt
    12421 ctttaattat tagtgtagcc gttctacttt ctgctctccg cactgcagaa aataaattgg
    12481 gctctcaatt gtcattgatt gcgttcgatg ctacaaaatc agctgcagag aacattgttc
    12541 ggcaaggcct ggcagcccta tcatcaagca ttactggagc agtcacacaa gtaggtataa
    12601 cgggtatcgg tgctaaaaaa acgcattcag ggattagcga ccaaaaagga gccttaagaa
    12661 agaaccttgc cactgctcaa tctcttgaaa aagagcttgc aggttctaaa ttagggttaa
    12721 ataaacaaat agatacaaat atcacctcac cacaaactaa ctctagcaca aaatttttag
    12781 gtaaaaataa actggcgcca gataatatat ccctgtcaac tgaacataaa acttctctta
    12841 gttctcccga tatttctttg caggataaaa ttgacaccca gagaagaact tacgagctca
    12901 ataccctttc tgcgcagcaa aaacaaaaca ttggccgtgc aacaatggaa acatcagccg
    12961 ttgctggtaa tatatccaca tcaggagggc gttatgcatc tgctcttgaa gaagaagaac
    13021 aactaatcag tcaggccagc agtaaacaag cagaggaagc atcccaagta tctaaagaag
    13081 catcccaagc gacaaatcaa ttaatacaaa aattattgaa tataattgac agcatcaccc
    13141 aatcaaggaa ttcggcagcc agtcagattg ctggtaacat tcgagcttaa tatatccaag
    13201 agccataata atatatggct cttcctgtaa ggaaataacc atgaatataa caactctgac
    13261 taatagtatt tccacctcat cattcagtcc aaacaatacc aacggttcat caaccgaaac
    13321 agttaattct gatataaaaa caacgaccag ttctcatcct gtaagttccc ttactatgct
    13381 taacgacacc cttcataata tcagaacaac aaataaggca ttaaagaaag agctttcaca
    13441 aaaaacgttg actaaaactt cgctagaaga aatagcatta cattcatctc agattagcat
    13501 ggatgtaaat aaatccgctc aactattgga tattctttcc aggaacgaat atccaattaa
    13561 taaagacgca agagaattat tacattcagc cccgaaagaa gccgagcttg atggagatca
    13621 aatgatatct catagagaac tgtgggctaa aattgcaaac tccatcaatg atattaatga
    13681 acagtatctg aaagtatatg aacatgccgt tagttcatat actcaaatgt atcaagattt
    13741 tagcgctgtt ctttccagtc ttgccggctg gatctctccc ggaggtaacg acggaaactc
    13801 cgtgaaatta caagtcaact cgcttaaaaa ggcattggaa gaactcaagg aaaaatataa
    13861 agataaaccg ctatatccag caaataatac tgttagtcag gaacaagcaa ataaatggct
    13921 tacagaatta ggtggaacaa tcggcaaggt atctcaaaaa aacgggggat atgttgtcag
    13981 tataaacatg accccaatag acaatatgtt aaaaagctta gataatctag gtggaaatgg
    14041 cgaggttgtg ctagataatg caaaatatca ggcatggaat gccggattct ctgccgaaga
    14101 tgaaacaatg aaaaataatc ttcaaacttt agttcaaaaa tacagtaatg ccaatagtat
    14161 ttttgataat ttagtaaagg ttttgagtag tacaataagc tcatgtacag atacagataa
    14221 actttttctc catttctgag gtgcgccatg cataatgtaa ataatactca agcgccaaca
    14281 ttcttatata aggcaacttc accatcatca acagaataca gcgagttaaa aagcaaaata
    14341 tccgatatcc atagttcgca aacttctcta aaaacaccag catcagtgtc tgaaaaagaa
    14401 aactttgcaa cgtcttttaa tcagaaatgt cttgattttt tattttcttc ctcagggaaa
    14461 gaagatgtgt taagaagcat ttattccaac tcaatgaatg cgtatgccaa aagcgagatt
    14521 ctcgaatttt caaatgtttt gtactcctta gtacatcaaa atggtcttaa ttttgaaaac
    14581 gaaaagggac ttcaaaaaat tgtcgcacag tattcggaac taattataaa agataaatta
    14641 tcccaagatt ctgcctttgg accatggtcg gcaaagaata agaaactcca tcaattacga
    14701 caaaacattg agcacagact tgcactatta gcacaacaac acacatctgg tgaagcttta
    14761 tcattgggac aaaaactcct caatactgaa gtatcatcat ttatcaagaa taatattctt
    14821 gctgaattaa agttaagtaa tgaaactgtt tcatctctca aactagatga tttaattgac
    14881 gcacaggcaa aacttgcctt tgatagtttg cgcaatcaac gtaaaaatac tattgatagt
    14941 aaaggatttg gtataggtaa actgtcaaga gacttaaata cagtagccgt gtttcctgag
    15001 ctgttgagaa aagtccttaa tgatatttta gaagatataa aagattcgca tcctatccaa
    15061 gatggcctcc ctacacctcc cgaagatatg ccagatggcg gaccaacccc cggagccaat
    15121 gagaaaacat cccaacctgt aattcactat catataaata atgataatag aacttacgat
    15181 aatagagttt ttgacaacag agtatatgac aatagctatc acgagaaccc agaaaatgat
    15241 gcacagtctc ctacttctca gacaaacgat ctattatccc gtaacggaaa ctcattacta
    15301 aatccacaaa gagcactagt tcaaaaagta acttccgttc taccacactc tatatcagat
    15361 actgtccaga catttgcaaa taattcagct ttagaaaagg ttttcaacca tactccagat
    15421 aattcggatg gaataggttc agacctgtta actacgagta gtcaagaaag atctgcaaat
    15481 aactctcttt ctcggggaca caggcctctg aacatacaga actcttcaac cacctcccct
    15541 ctccacccgg aaggagtgac aagcagtaat gataactcat cagatacaac taaaagtagc
    15601 gcttctcttt ctcatagagt agcttcgcaa ataaataaat tcaactcaaa cactgattca
    15661 aaagtacttc agactgattt tttatcaaga aatggagaca catatttaac acgggaaacg
    15721 atatttgaag cttcaaaaaa agtaacaaac tccctaagta atcttatatc tctcattgga
    15781 actaaatcag gaacacaaga acgagagtta caggaaaaat caaaggacat tacaaaatcc
    15841 acaacagaac acagaataaa caacaaatta aaagttacag atgcaaataa aataaactac
    15901 gtaacagaaa ccaacgcaga tacaattgat aaaaatcatg cgatctatga aaaggcaaaa
    15961 gaagtatcta gcgccctcag caaggtattg tcacaaattg acgatacctc tgcagaatta
    16021 cttacagatg atatatctga tttaaaaaat aacaatgata ttacagctga aaacaataat
    16081 atatataaag cagcaaaaga tgtaaccact tccctatcaa aagtattaaa gaatatcaat
    16141 aaggattaaa atatgataaa agaaaaaata ttatcaatag tggccttttg ctatggtata
    16201 gcgtacagca aactatctga agaaactaaa tttattgaag atctttctgc cgattctctt
    16261 tctctgattg aaatgctgga tatgattagt tttgagttca atctacgtat agatgaatct
    16321 gcattagaac acattattac tattggagat cttatttctg tagtcaaaaa tagtacaaaa
    16381 tcaatatagt aattgataag cattttttca tctatggagc tctcacatca gagctccaca
    16441 agaatattat tcttttatcc aataaagata aattgcatca atccagctat taaaatagta
    16501 atttttaaga ctaccgttga ctatcatcaa cagttacata caatcttgct cacactgcat
    16561 ttaacttttg tcaatataaa tcaatatgaa taaacagggt gtgatatggt ggatttgtgc
    16621 aacgacttgt taagtataaa ggaaggccaa aagaaagagt ttacactcca ttctggtaat
    16681 aaagtttcct ttatcaaagc caagattcct cataaaagga tccaagattt aaccttcgtc
    16741 aaccaaaaaa cgaatgtacg cgatcaagaa tccctaacag aagaatcatt agccgatatc
    16801 ataaaaacta taaagctaca acaattcttc cctgtaatag gaagggagat tgatggtaga
    16861 attgaaattc tggatggcac tcgtagaaga gcatctgcaa tatatgcagg agcagatctt
    16921 gaagttctat attcaaaaga atatatatct actcttgatg ccagaaaact agcaaacgat
    16981 atacaaacag caaaagagca tagcatccga gaacttggta ttggtcttaa ttttctgaaa
    17041 gtatcaggga tgtcctataa agacatagcc aaaaaagaga atctgtctcg cgcgaaagtc
    17101 actcgtgcct ttcaggcagc aagcgttcca caggaaataa tatctctatt tccaatcgcg
    17161 tcagaactta actttaatga ctacaagata ttattcaatt attataaagg ccttgaaaag
    17221 gctaatgaat ctcttagttc tacactacca atattaaagg aagaaataaa agaccttgat
    17281 acaaatttgc ccccggacat atataaaaaa gaaattttaa acatcataaa gaaaagcaaa
    17341 aacagaaagc aaaacccttc gctaaaagtt gactcgttat ttatttctaa agacaaacgg
    17401 acttacataa aaagaaaaga gaataaaaca aacagaactc taatatttac attatctaaa
    17461 ataaacaaaa cagttcagag agaaatagat gaagctattc gggatataat ttctcgccat
    17521 ctatcgtctt cataaacatc gaacactgat gtaaactgcc caattcagat aataaaaatc
    17581 ttcgttgaac ttgactttca gccggtgggg taattccgtc ggctatctgt tattcaatcc
    17641 cctcgcactg tgtttcctta aaattacggt gaaaactcat accctgctga ccacgagaaa
    17701 attatctggt gaaattagta atattcatat cattgaaaat gaattgtgca actaagggat
    17761 gcggcatgat ggtcaaaatt ttatgtgaag atgagtgact ggccgaaaat attcagcgta
    17821 tttttaatcg tttcaataaa aacagcgagg attagcagtc aacgctttta ctactcaatt
    17881 tttttacatg gtgataaaca aaaaatccac cattaacaga cttcgttcac agtatcaaaa
    17941 aagctttaag aatatattag cagatttctc aaatataatt actgttttat tctcctaatc
    18001 ttcccataaa atgaccgact tacattattt tataattata aacaagtttg attttaaaat
    18061 catattaatt attaacaaga gatttatttt tctaacccca aaagttatgc ttagtcaaag
    18121 agtaacttat aatatccata cccctcatag ggcttacagt ttaaattgcg ggtataaaat
    18181 acatctattt tcaaataaaa taaagcatac tccaattaca acgagtgact aaaataaaaa
    18241 tatcatccaa gtcaatcact gtttgctctg caaaatatgt cagccttctc aaacttacca
    18301 gcacttaaat atttaagcac acaatcattc cttgagggag ttcacttatt tcaagagaaa
    18361 catttcgtct tcccacacaa ctaatccgcc caatatagct aaatttgtac tcagtaatat
    18421 catttgttat gcccccccgc cccaaaatat tcaagctcct tcacagataa aaaacgacaa
    18481 tttacaaagc ctcattagtt ataactatgg aaatacctat tttagtatat cctataaagt
    18541 tagaattact tcttcttgca cctaattcca tagttgaaaa acaagaataa ttttctccta
    18601 cagctggatc catatagctt ccatcaggtc tacacaacac ccagtggagg ccgacaggaa
    18661 ctccaataaa ggatacagca acagcttcca aaactctttg gttagaagat aacacatctc
    18721 gttgatgtac tatattacat cccatgccaa ttaataagtc ccttgcatcg ggataaataa
    18781 aactaattac ttgcgaaaaa atgttacttt cttcaacgac ataagcattc aatccaagaa
    18841 gcctcgtagc catgactatc ttatcaggca tagaataacc ggcatccgcg atattatctt
    18901 tgtgtattct cggattataa ttaccactag ttattaagta taaatcccgc tcacaacgat
    18961 tatccaaatc aagagaactc ttccttgtca tttcggacat agaccctttg tattgtggta
    19021 ttttatcaac ccctaattcc tttgcagcac agagtaaact ggcagctcca caagaattgt
    19081 catacatctg cttcctcgcc aaaaaacaac cttgttcgtt tactctattc tcatttaccc
    19141 tcacatcagt ctgatgagat atcatatatt ctcttggtat agccccctgc aataaaaccc
    19201 catataacat aacacctgta tgaatacatc cccgtttgca aggcttccgt tgttccgaca
    19261 tcgacgagta attgcagcac agtattgcat ccagcaatgc gaactactat gagaattagc
    19321 tgaccgggtc cttattcccc gaacgaactt taaatccaaa acctcatact cttttccgtc
    19381 gtcatcacgt ccgtcaccat aacaaccttt cagcatccac ccttttatta agagttaatg
    19441 tccatcagca tcagcaatta ataccgcctg atactgacat atttttgtcg tatttacgtc
    19501 cataatcggt tattgtgata caggtcacac aagcttattt gctattgtta caataaccac
    19561 cttggttgag agctgttttt tcaactttgt tatcaactcc cccaactcct tggtcagtaa
    19621 ggctgccgac cgagcacagt gaatagatag tatccgcagg taccaccatg cttacttaat
    19681 actcagaagg cgaagtgctc tccaattaac tctgccgcac tatgcttata agctgctttt
    19741 caatgccgtc tatcaacatg tccagttcat tcaatctgtt gtactggtaa tccaacgcag
    19801 taatgatata tgcaggtatt ctttcatcca ctccagtaac gtttcatgca aagcattaat
    19861 ttacgcgcta cggaacttca ccagttgact gagcaaacga tgtgggacca gtactgcctg
    19921 cagttcttct attgttacag cgatttcttt accaaacagc tgaaccacca tcaagataaa
    19981 gatagcctga acattcatcg caccattttg ttaccaatgg caaaagattt aacgaaccgt
    20041 gcctgcatca ggcggacttt ttatccctgc tttgttagct ccctaacctt gtacagagag
    20101 cctccataag ctcccatatc aagcagacat ggttcgcagt tactgacata caccagaaaa
    20161 ctctgacagc agactcactt atcctccact tcactcatat ccttattggt gagatgaacc
    20221 tgaatgacag gttttgcgac ataaacgcca accagtgtat atttcacctg tagagattct
    20281 acaatctgta gcgttatgca tctaatgtta ggctctatga tgtcagaaat ctgcgagact
    20341 ctacaccttc tgcatttacc tttatttatc accatgctcc tcagttaacg gagaacgtgt
    20401 tcattgcatt ttgtcactta catttcgcct ttggtatcgg ggggatatag gttccaacgt
    20461 tccgcatgag aacctgaacc ggcttcacgc caccttgata ccagatgccg cacaggtagt
    20521 caacaagtgt ca
//
LOCUS       DAJTRQ010000067        20504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000067 DAJTRQ010000000
VERSION     DAJTRQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="OO332_002462"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="OO332_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5942906.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="OO332_002463"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="OO332_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5942907.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="OO332_002464"
     CDS             complement(977..1267)
                     /locus_tag="OO332_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5942908.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="OO332_002465"
     CDS             1701..2411
                     /locus_tag="OO332_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5942909.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="OO332_002466"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="OO332_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5942910.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="OO332_002467"
     CDS             complement(3539..4198)
                     /locus_tag="OO332_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5942911.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /gene="bisC"
                     /locus_tag="OO332_002468"
     CDS             4351..6684
                     /gene="bisC"
                     /locus_tag="OO332_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5942912.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="OO332_002469"
     CDS             complement(6653..7093)
                     /locus_tag="OO332_002469"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5942913.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="OO332_002470"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="OO332_002470"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5942914.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="OO332_002471"
     CDS             7811..8509
                     /locus_tag="OO332_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5942915.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="OO332_002472"
     CDS             8738..9946
                     /locus_tag="OO332_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5942916.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="OO332_002473"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="OO332_002473"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5942917.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="OO332_002474"
     tRNA            12053..12129
                     /locus_tag="OO332_002474"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="OO332_002475"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="OO332_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5942918.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="OO332_002476"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="OO332_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5942919.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="OO332_002477"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="OO332_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5942920.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="OO332_002478"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="OO332_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5942921.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="OO332_002479"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="OO332_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5942922.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="OO332_002480"
     CDS             complement(18744..20015)
                     /locus_tag="OO332_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5942923.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4912 a         5077 c         5389 g         5126 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatagcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggcctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aatcggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc cggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atgacgctcg caga
//
LOCUS       DAJTRQ010000068        20169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000068 DAJTRQ010000000
VERSION     DAJTRQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(66..4526)
                     /gene="mukB"
                     /locus_tag="OO332_002481"
     CDS             complement(66..4526)
                     /gene="mukB"
                     /locus_tag="OO332_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5942924.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4526..5230)
                     /gene="mukE"
                     /locus_tag="OO332_002482"
     CDS             complement(4526..5230)
                     /gene="mukE"
                     /locus_tag="OO332_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5942925.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5211..6533)
                     /gene="mukF"
                     /locus_tag="OO332_002483"
     CDS             complement(5211..6533)
                     /gene="mukF"
                     /locus_tag="OO332_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5942926.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6530..7315)
                     /gene="cmoM"
                     /locus_tag="OO332_002484"
     CDS             complement(6530..7315)
                     /gene="cmoM"
                     /locus_tag="OO332_002484"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5942927.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7451..8230
                     /gene="elyC"
                     /locus_tag="OO332_002485"
     CDS             7451..8230
                     /gene="elyC"
                     /locus_tag="OO332_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5942928.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8207..9100)
                     /locus_tag="OO332_002486"
     CDS             complement(8207..9100)
                     /locus_tag="OO332_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5942929.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9254..10000)
                     /gene="kdsB"
                     /locus_tag="OO332_002487"
     CDS             complement(9254..10000)
                     /gene="kdsB"
                     /locus_tag="OO332_002487"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5942930.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9997..10179)
                     /gene="ycaR"
                     /locus_tag="OO332_002488"
     CDS             complement(9997..10179)
                     /gene="ycaR"
                     /locus_tag="OO332_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5942931.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10231..11463)
                     /locus_tag="OO332_002489"
     CDS             complement(10231..11463)
                     /locus_tag="OO332_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5942932.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11500..12486)
                     /gene="lpxK"
                     /locus_tag="OO332_002490"
     CDS             complement(11500..12486)
                     /gene="lpxK"
                     /locus_tag="OO332_002490"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5942933.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12483..14231)
                     /gene="msbA"
                     /locus_tag="OO332_002491"
     CDS             complement(12483..14231)
                     /gene="msbA"
                     /locus_tag="OO332_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5942934.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14268..16487)
                     /locus_tag="OO332_002492"
                     /pseudo
     CDS             complement(14268..16487)
                     /locus_tag="OO332_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16694..16978)
                     /gene="ihfB"
                     /locus_tag="OO332_002493"
     CDS             complement(16694..16978)
                     /gene="ihfB"
                     /locus_tag="OO332_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5942935.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17138..18811)
                     /gene="rpsA"
                     /locus_tag="OO332_002494"
     CDS             complement(17138..18811)
                     /gene="rpsA"
                     /locus_tag="OO332_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5942936.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18922..19605)
                     /gene="cmk"
                     /locus_tag="OO332_002495"
     CDS             complement(18922..19605)
                     /gene="cmk"
                     /locus_tag="OO332_002495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5942937.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19778..>20169)
                     /gene="ycaL"
                     /locus_tag="OO332_002496"
     CDS             complement(19778..>20169)
                     /gene="ycaL"
                     /locus_tag="OO332_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5942938.1"
                     /translation="NEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVAS
                     QLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGG
                     RTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4833 a         5594 c         4921 g         4821 t
ORIGIN      
        1 cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt gctgccgcct
       61 taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc agggagttgc
      121 ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa gactttacgt
      181 accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc gatgatgagc
      241 tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc atccagtcgc
      301 gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg caggcggcga
      361 gattcatctt cccagctttg taccaccatc accagaatcg acataccggt accaatcgcc
      421 tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc acggttaacc
      481 tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat ggtctgcggc
      541 gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc caacgcttcc
      601 gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg ttcagagagc
      661 acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac gctgttcacc
      721 tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt ctgctcgcgc
      781 tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag tttctgttca
      841 cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc gatagcttcc
      901 accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg ctgataaacc
      961 gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat gcgcagcacg
     1021 tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc cttatccgac
     1081 atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa gcggcgctca
     1141 acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc ggtcactacc
     1201 tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt caggttgtcc
     1261 atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga acggttattg
     1321 ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc cccgctatca
     1381 gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc ttttttggtg
     1441 tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg cgctgcgtga
     1501 ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag acgttcacgc
     1561 agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga atagctaaag
     1621 tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc atcgcgctgc
     1681 atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg gtcgctctgc
     1741 aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg aacaaaacgc
     1801 gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc cgccaggctg
     1861 tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac gccttctttc
     1921 gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact caacgcccgc
     1981 tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc agactcaaac
     2041 gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg ctgggttttc
     2101 tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc ggcatggagg
     2161 ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg gaaacgtgaa
     2221 taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc atcaacgctg
     2281 aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc ttccgggcaa
     2341 tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac gatggcgtgg
     2401 cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac gtcgtcataa
     2461 atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg ctgatcttca
     2521 gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt gcgcgcgcct
     2581 acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg cagatattca
     2641 gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg gttgagactg
     2701 ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg agactgcagc
     2761 tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac gctatcggaa
     2821 agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc aatatcaaaa
     2881 ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg ctgctcacgc
     2941 agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc tgccaggtga
     3001 cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc gttacgcgcc
     3061 agcgggccgt tgattgccac caccagctga taagcctgct caaactggct gtgcgcggtt
     3121 tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc cagctctttc
     3181 gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg cagatggcac
     3241 agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc gcgcgtttgc
     3301 tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc cacttccagt
     3361 tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc tgccaccact
     3421 tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta gcgttcgatc
     3481 ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc cgcctgataa
     3541 tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc catatcgacg
     3601 tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg acgaaactcc
     3661 agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc cgccgccacg
     3721 tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg ggtgacacga
     3781 atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg gaacgcttta
     3841 cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat ggcactggag
     3901 atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc agatgcggag
     3961 cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc ggtaatggag
     4021 ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag ctcgttaagc
     4081 ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt cggctgcacc
     4141 gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc gcgtccggca
     4201 acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat ggtgtcgagc
     4261 atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg cgaaccgctg
     4321 gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg gatcagcgcc
     4381 gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga aagcgtcgtg
     4441 accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat cagcgtcagt
     4501 gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc tgactctctt
     4561 cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca tcacgaatca
     4621 ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg aagcggaaca
     4681 ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc atgcctaaac
     4741 gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg tcaacgtctg
     4801 aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc agggtgagca
     4861 gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt tccgggctga
     4921 gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag acggaacgcg
     4981 ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg cgaataagct
     5041 caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc aggaatgcat
     5101 gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg tccagcgccg
     5161 gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt tcaatatttg
     5221 tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca tttcgctggc
     5281 agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat aacaatacgc
     5341 gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac caccagacca
     5401 agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat gatcgccgcc
     5461 agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag ttccccagtc
     5521 acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg atcggcattg
     5581 gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg taaccgctga
     5641 gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg tacgtggcgg
     5701 tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg atcgagtttg
     5761 ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt catcgtcgca
     5821 tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag cgtatcctgc
     5881 aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga aatcgccgcc
     5941 cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg ctgttcgtcc
     6001 atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata tttcagtggc
     6061 gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc atctgctgcg
     6121 cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt agaaaactcg
     6181 cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg gtaaattgcg
     6241 ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac catgtcgttg
     6301 atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc atcactcaca
     6361 tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg ctcgccgttc
     6421 agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat ggagaagtca
     6481 ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact catactttat
     6541 ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta atatacggtt
     6601 cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg cgctgctggt
     6661 gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata atttgccaac
     6721 cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct ggcgaaagcg
     6781 tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg gcgaccatgt
     6841 tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg cctggacgca
     6901 acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg agcaccgcat
     6961 ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga gcggcgcaat
     7021 gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt gcgcggtcga
     7081 tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc attttgattg
     7141 cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg cccatttccg
     7201 ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct ttggtggtgc
     7261 cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc tgcattgctt
     7321 tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac agccacggcg
     7381 agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt taaaaaagga
     7441 accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc tgccacttcc
     7501 gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc gttttcaaaa
     7561 aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga gtttgcaacc
     7621 ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga ataattcgca
     7681 aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc agtgggcacc
     7741 gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc gtttatggcg
     7801 cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca atacggtgag
     7861 tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg agcaaattat
     7921 caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac aggcgattgg
     7981 tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga tgatcttttt
     8041 tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca tcgactcgcc
     8101 gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata gcgatcgtgt
     8161 tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag gcgagccacg
     8221 gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg gtattaacca
     8281 gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac gactccgcca
     8341 ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta tccattagtc
     8401 ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc atcgccagta
     8461 actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg tgaatcaaca
     8521 cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg cgaaacagga
     8581 tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg gtccatagca
     8641 cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg tcgaccgcgc
     8701 cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg atttggtctt
     8761 tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca ccacgcatcc
     8821 gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca ccataaatag
     8881 tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc agttgtcggg
     8941 cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc tggctgtcat
     9001 acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga ctgagttttt
     9061 cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc ctcataaaag
     9121 ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga tcaattgttg
     9181 aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt ggctgaagtg
     9241 tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca ggggtatcca
     9301 cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg taccacagaa
     9361 cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag ttgacgtaac
     9421 gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag ttatcgccaa
     9481 cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca cgtgagaagt
     9541 acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta aacgcttctt
     9601 ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga gcgaggttat
     9661 cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc acattaacga
     9721 tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt tctgttcctg
     9781 actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg gcgcgggcaa
     9841 catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc gcgcgttcaa
     9901 gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg ggcagacgcg
     9961 tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct catcagcagt
    10021 cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg ggaaagccag
    10081 gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt ttccgttgca
    10141 aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag atggatcggg
    10201 ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt cccagccagt
    10261 gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg ttgcctgctg
    10321 ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg tcgttggttt
    10381 aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc gatgcatttt
    10441 ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa aatagccata
    10501 ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa aaagctgctc
    10561 tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc tgtgcgttgc
    10621 agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat gcagccaaag
    10681 attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc tcgcttcgcg
    10741 ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact gttcgcggaa
    10801 tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg tttgcgaaac
    10861 gagatcgcgc tcatcatccc agtcaggcat gactcggtgg gttaaatcat aaacgcgctg
    10921 gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt ccagatgccg
    10981 tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct caaaatcggc
    11041 tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt ccgcctcatg
    11101 ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca gcatgcggtg
    11161 gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc agtattccat
    11221 taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc ccagacgact
    11281 gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt gcagcaagga
    11341 catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg gtttgtttaa
    11401 caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt gcggcagcga
    11461 cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag aagccagcga
    11521 ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt ctacaggcaa
    11581 ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt cagtcattac
    11641 cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag actgatgatc
    11701 ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg caaaaaagcg
    11761 cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa cgtcacaccg
    11821 cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga tttcaccgct
    11881 gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc ccgctcgctc
    11941 acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac catcaataac
    12001 gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg ttacgatgat
    12061 ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa cgggagaaac
    12121 cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg cctgtgctgt
    12181 ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat atccccgcga
    12241 tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga caacgaccgg
    12301 ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta cgggggcacg
    12361 ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca ccagaccata
    12421 caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc agattttttc
    12481 gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg ctcaagcaaa
    12541 tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat ttcgtctgcc
    12601 ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt ctgcaactca
    12661 tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt agcttcatcc
    12721 agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg ctgacgctga
    12781 ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt atccatctta
    12841 ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc acggctgtac
    12901 tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag atggacattc
    12961 tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg cagatcgtga
    13021 ccatccatca ggatttcacc ttcatcaata tcgtaaaaac gcgtgatcag gctggcaatg
    13081 gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc cggaattttc
    13141 aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt gacattgcgg
    13201 aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt ctcctgctca
    13261 ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa ctgggcgtta
    13321 acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa aacaacggta
    13381 atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata cagaacaaac
    13441 gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga ggctgaaacc
    13501 attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt cgtttccact
    13561 tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc tgcactggtg
    13621 gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg cttcgatact
    13681 acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga cagttgccaa
    13741 ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg cacaacagta
    13801 atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat acgtgacaac
    13861 agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat gtgaccaaac
    13921 aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca gtagctggag
    13981 acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg catccacacc
    14041 agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag gagcgataac
    14101 atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat cagacccgct
    14161 ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga gagatctttg
    14221 tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta cccgttatcc
    14281 actggcacgc caaaccactg ataataccaa cgaggtaaaa tttgctctct gaggctgcta
    14341 atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg agtatcaagc
    14401 cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa tcgccatgcg
    14461 ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat taatggcaac
    14521 gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg cacttgctgc
    14581 cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt tagaagaatg
    14641 ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt atcgttgcta
    14701 ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc ttcgccacgc
    14761 acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat cggctatata
    14821 tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag aataacgcct
    14881 tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc actatcccct
    14941 tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc tatcaccatt
    15001 gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg aggtttttgc
    15061 cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa cgttcgccag
    15121 gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca ttgccaacat
    15181 tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag gattaacggc
    15241 ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15301 ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15361 tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca
    15421 cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt
    15481 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15541 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15601 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15661 cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag
    15721 tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac
    15781 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc
    15841 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15901 accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    15961 aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc
    16021 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16081 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg
    16141 ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga
    16201 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16261 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16321 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16381 gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac
    16441 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgcc cccctccctg
    16501 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16561 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16621 tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16681 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16741 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16801 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16861 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16921 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    16981 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17041 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17101 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17161 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17221 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17281 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
    17341 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17401 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17461 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17521 gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17581 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17641 ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga
    17701 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17761 ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata tccagaacca
    17821 taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17881 aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc
    17941 cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18001 cgatagctac ccacggatct tcgcccagct gtttcaagcc cagggataca cgggtacgtt
    18061 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18121 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18181 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18241 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18301 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18361 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaaccaggca
    18421 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18481 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18541 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18601 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18661 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18721 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18781 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18841 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18901 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    18961 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19021 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19081 caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19141 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19201 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19261 acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac
    19321 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19381 attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga
    19441 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19501 gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc
    19561 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19621 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19681 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19741 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19801 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19861 gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc
    19921 cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt
    19981 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20041 ccacgccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca
    20101 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
    20161 tttcgttgt
//
LOCUS       DAJTRQ010000069        19916 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000069 DAJTRQ010000000
VERSION     DAJTRQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..746)
                     /locus_tag="OO332_002497"
     CDS             complement(<1..746)
                     /locus_tag="OO332_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5942939.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            1131..1319
                     /locus_tag="OO332_002498"
     CDS             1131..1319
                     /locus_tag="OO332_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5942940.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1270..1965
                     /locus_tag="OO332_002499"
     CDS             1270..1965
                     /locus_tag="OO332_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR5942941.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(2077..2424)
                     /locus_tag="OO332_002500"
                     /pseudo
     CDS             complement(2077..2424)
                     /locus_tag="OO332_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="OO332_002501"
                     /pseudo
     CDS             complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="OO332_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4431..5489
                     /gene="citC"
                     /locus_tag="OO332_002502"
     CDS             4431..5489
                     /gene="citC"
                     /locus_tag="OO332_002502"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5942942.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5504..5800
                     /gene="citD"
                     /locus_tag="OO332_002503"
     CDS             5504..5800
                     /gene="citD"
                     /locus_tag="OO332_002503"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5942943.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5797..6705
                     /gene="citE"
                     /locus_tag="OO332_002504"
     CDS             5797..6705
                     /gene="citE"
                     /locus_tag="OO332_002504"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5942944.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6716..8248
                     /gene="citF"
                     /locus_tag="OO332_002505"
     CDS             6716..8248
                     /gene="citF"
                     /locus_tag="OO332_002505"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5942945.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8252..8803
                     /gene="citX"
                     /locus_tag="OO332_002506"
     CDS             8252..8803
                     /gene="citX"
                     /locus_tag="OO332_002506"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR5942946.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8778..9656
                     /gene="citG"
                     /locus_tag="OO332_002507"
     CDS             8778..9656
                     /gene="citG"
                     /locus_tag="OO332_002507"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5942947.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9707..11170
                     /gene="citT"
                     /locus_tag="OO332_002508"
     CDS             9707..11170
                     /gene="citT"
                     /locus_tag="OO332_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR5942948.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11284..12090
                     /gene="rna"
                     /locus_tag="OO332_002509"
     CDS             11284..12090
                     /gene="rna"
                     /locus_tag="OO332_002509"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5942949.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12319..12729
                     /gene="rnk"
                     /locus_tag="OO332_002510"
     CDS             12319..12729
                     /gene="rnk"
                     /locus_tag="OO332_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5942950.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12960..14197)
                     /locus_tag="OO332_002511"
                     /pseudo
     CDS             complement(12960..14197)
                     /locus_tag="OO332_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14418..14846
                     /gene="uspG"
                     /locus_tag="OO332_002512"
     CDS             14418..14846
                     /gene="uspG"
                     /locus_tag="OO332_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5942951.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="OO332_002513"
     CDS             complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="OO332_002513"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5942952.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="OO332_002514"
     CDS             complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="OO332_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5942953.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17712..18458
                     /gene="dsbG"
                     /locus_tag="OO332_002515"
     CDS             17712..18458
                     /gene="dsbG"
                     /locus_tag="OO332_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5942954.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18668..19570
                     /locus_tag="OO332_002516"
     CDS             18668..19570
                     /locus_tag="OO332_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5942955.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19717..19916
                     /locus_tag="OO332_002517"
                     /pseudo
     CDS             19717..19916
                     /locus_tag="OO332_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5016 a         5124 c         4758 g         5018 t
ORIGIN      
        1 ataacttgcg aaccgcaaag ccagttcccg ttattatcca tgtacgcctg accatcggtg
       61 ccattggctg tccttgagtt attaatcatg tagatgccaa attgcctatt ccccagccca
      121 ccaattacga atttacggtc ggtatggtcc tggcgaagca atgcctgcgc accatcgttg
      181 gataccgcat tacggcccat aataacgttc tggtcacgca tatgaatcca catgcctgta
      241 ctgctgttaa ttaaaaaacg gtttgtaaat atatctcctg taacaaccag accatgcccc
      301 atgcttatcc ggccagttct gagattaagc gtaaaggggc gtagtggccc tatattacca
      361 ttttcccctt cattctctcg tgtagggatg atatgcaggc attcttcaga acgacgaaaa
      421 atggcaccaa aagatgaatt aaatattctc agtgcattga ccgtcgatat ttttacttca
      481 ctgctgaaaa gggctttaac aagaacatac agagcatccc atttaagatt catcaggtct
      541 ttaacccggc cgccagtgag gtggtgtctc catttgaaat actcattgcc gttgtcgcct
      601 gtttcaaacc acatgtatga atcagtatcg ctgtcggcat catttttaaa tccaatcttt
      661 gcccagtcag tattccgaat ccaggcaagg attgagtcgt tttcaaaagt aagtccaccg
      721 gacaacgtat cgccattttt ttgcacggcg ttaccagcct tgtttaccgt ttcctgtaaa
      781 ccgaggggcc gtttctggcc tgcatggcat gatttccgct tttaaacggg gaattcagcg
      841 tgctgattgg ctatgtaagg gtatcaacaa atgaccagaa tacagacctg caacgaaacg
      901 ctcttgtttg tgcaggatgt gaataaatat ttgaagataa attaagcgga acaaggacag
      961 accgaccggg attaaaacgt gctttaaagc gccttcaaaa aggaacgcat atagaaaacg
     1021 acgatcgaat caattaaatc gatctgtatt accgatcgat tatgtcccga taaccacacc
     1081 cgactgatga gattttttaa gataacggcg aattgatgca aggaggtgaa atgaaatcaa
     1141 ttcgctgtaa gaactgcaac aaactgttat ttaaagcgga ttcctttgat cacattgaaa
     1201 tcaggtgtcc gcgttgcaaa cgtcacatca taatgctgaa tgcctgcgag catcccacgg
     1261 agaaacattg tgggaaaaga gaagaaatca cgcattctga cgaaaccgtg cgttattgag
     1321 tatgaaggcc agattgttgg ctatggttca aaggagctgc gcgttgaaac catatcctgc
     1381 tggctggccc gcacaatcat tcagacaaag cactattccc gccgttttgt gaataactct
     1441 taccttcacc tgggagtatt cagcgggcgt gatctggttg gcgttctcca gtggggatat
     1501 gcccttaacc ccaactcagg tcgtcgtgtc gtgcttgaaa cggataaccg gggctatatg
     1561 gagttgaatc gcatgtggct acacgacgac atgccccgca actctgaatc acgggccatc
     1621 agctacgcgc tgaaagttat cagattactg tatccgtcag tggagtgggt tcagtccttt
     1681 gctgacgaac gctgcggact cgcaggcgtt gtgtatcagg cgtcgaattt tgattttatt
     1741 ggcagccatg aaagcacgtt ctacgagctg gatggtgagt ggtatcacga gataacgatg
     1801 aacgcgatta agcgcggagg gcaacgaggc gagtatttgc gagctaataa ggagcgtgcc
     1861 gtggtgcaca aatttaatca gtatcgctac atcagattcc tgaacaagcg agcaaggaag
     1921 cggctaaata ccaaactatt caaggttcag ccatacccta agtgatcccc atgtaatgaa
     1981 taaaaagcgg taattaatac tgctgtttca tttgaagcgc gaaagctaaa gttttcgcat
     2041 ttatcgtgaa acgctttcgc gtttttcgtg cgccgcttca cccagccgtt cataggcaat
     2101 tggcttaatg agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc
     2161 actggctgca gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcag
     2221 taaattaatc cctctaccgt caggaagata gttatcgagc aagattagcc ccggcttaaa
     2281 acgctcgatc atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc
     2341 gggaatgtga cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat
     2401 caatagggtt aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg
     2461 taccgcaggg atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg
     2521 caatcaagta caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgacccctg
     2581 ctcaaatatt ttgtctcgta gagactctgg aacgccgcag ccctgatcgg cgacttcaat
     2641 caccacatca tcgccttcat cgctgaggaa taattcaacg ctcttgtttc cttcatcgct
     2701 acgcaggctg gcttcgaagg cgttatcaag taaattgcca acaatggcgg caaactcggt
     2761 gctatccagt cctggcggca gttgcgaaag ctggctaccg gggacgatga tcatttttag
     2821 ccccagttcg cgggcgcgct gcactttacc aaaaagcagc cccgccacct ggcgatcggc
     2881 aaacgcctcg cgcaggctgt caataagctg ttgctgggcc tgagactccc cctgcaccat
     2941 cgccagcacg cgatcatact ctttcatctg caacagacca ttgagagtcg acatccaatt
     3001 gagatgctcg tgtcgcaatg tacgaaggct ctcaacgtat tgttttattt gcgtcagttg
     3061 cgcattgagg gtggaaattt cgtctttact gcgaaagctg ataatggctc ccagcaaatc
     3121 atcacctgaa cgaatagctt cccgattggc aataacgctc agaccgttaa agttagccac
     3181 cacatcctga cgtttttcat caatctgttc ggtaaagaaa tcggcagacc tgaccacttc
     3241 agcaatgggt ttacccaacc attgccgtcc aggggaactc agccccagca tctttcttgc
     3301 gttacgattg atggcggtaa tgtaaccgtg cggatccacc gcaatcagcc cttcataaac
     3361 cgaactaaac agcgcctctt gctgacggac cacccgtgcg atttgctttg gctccatgcc
     3421 catcatctgc cgacggatat gcgcggccag gaaccacgac agcaacatca gaatccctaa
     3481 cagcacgaca aacacacctg ccatcggtaa taaaaactca gcccgccagc tatcgatttt
     3541 actcaccagg tagccaatcg acaccacgcc gatgactttt ccatcgtcat caaagattgg
     3601 cgttttggcg cgcatcgcca tacccattga ccctttcccg gtaatgaagt agctctcccc
     3661 tttctccagc gcgccctgtt tggtgaactg cataggataa ccaattttct ccggattagg
     3721 atggtaaagg cggatcgagt gccggtcgcc aatcaccaca taatcaaaat cggtatctct
     3781 ttgtaatttg ttagcgatgg tcgccagccg tttgtagtca cgcgttttca ccgcagagat
     3841 gacactgtca ttggaggcaa taattttcgc ctgattcatt gccatgtcgc gtacatgaag
     3901 cgttaaatag tcctcaaaac tggccgtaaa atattgcgcc agggctgcaa tgacaaatat
     3961 tgagaacacc agaatcagca aaaagatacg cagcggaaat gtcagtcttt ggaaaaatgc
     4021 aaactgttta ttctcgttaa gctgcaacat tgttttcctt gcgggttgtc ccgagcctct
     4081 ttgacggatg taattaaaat gttgaatcaa aatacataga tattaattat gatcgtggca
     4141 aattttattc attaaatcaa ttaaatcact taaacctgcg agtcatttcg taattacatt
     4201 aattaaaaaa cttaaaacca ttaaacaaat aaaaaaacca ctaactctat gtgaaataaa
     4261 tcaaaatttc acgccgaaat actccttagg atgtatagcg aaaggagaaa aagatatacc
     4321 tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcagtttgca acattgaaca
     4381 acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt atgttcgtca
     4441 atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa atcgcccaat
     4501 tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt accgtaaccc
     4561 gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa tattattaaa tgcgttgcta
     4621 tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt agccactgaa ctgataaacc
     4681 tcgcctatga gcggcacagc acgcatctgt ttatttatac caaaaccgaa tacgaggcgc
     4741 tgttccgcca gtgcggtttt tccacgctga cctgcgtacc cggcgtgatg gtgctgatgg
     4801 aaaacagcgc cacgcgactg aaacgctatg ccgaatcgct gaaaaaattg cgtcatccag
     4861 ggaacaagat tggctgcatt gtgatgaacg ccaatccctt tacgaatggt caccgttatc
     4921 tgattcaaca agctgcggca cagtgcgact ggtttcatct gtttttagtc aaagaagatt
     4981 cttcacgctt cccctatgaa gaccggctgg atctggtgtt aaaaggcacc gccgatattc
     5041 cacgcctgac tgtgcatcgc ggctcagaat acatcatctc ccgcgctacg ttcccttgct
     5101 acttcattaa agaacagagc gtcattaacc attgttacac cgaaattgat ctgaagattt
     5161 tccgtcagta cctcgctccc gcgcttggtg taactcaccg ctttgtcggt actgaaccct
     5221 tttgtcgcgt taccgcccag tacaaccagg atatgcgcta ctggctggaa acgccgacta
     5281 tctccgcagc gcccatcgaa ctggttgaaa ttgagcggct gcgttaccag gagatgccga
     5341 tatccgcttc ccgggtacgt caactgctgg cgaaaaacga tctcacggct atcgcgccgc
     5401 tggtccctgc agtcacgctg cattatttgc agaacctgct tgaacactcc cgccaggacg
     5461 cggcagctcg tcaaaagacc cccgcatgag aaacaggtga aaaatgaaaa taaaccagcc
     5521 cgccgttgca ggcacccttg agtctgggga tgtgatgata cgcatcgccc cactcgatac
     5581 gcaggatatc gacctgcaaa tcaatagcag cgttgagaaa cagtttggcg atgcaattcg
     5641 caccaccatt ctggacgttc tcgcccgcta caacgtgcgc ggcgtacagc tgaatgtcga
     5701 tgacaaaggc gcactggact gcattttacg tgcacgactg gaagccctgc tggcacgcgc
     5761 cagcggtatc ccggctctgc catgggagga ttgccaatga tttccgcttc gctgcaacaa
     5821 cgtaaaactc gcacccgccg cagcatgttg tttgtgcctg gtgccaatgc cgcgatggtc
     5881 agcaactcct tcatctaccc ggctgatgcc ctgatgtttg acctcgaaga ctccgtggca
     5941 ttacgtgaaa aagacaccgc ccgccgcatg gtttatcacg cgctgcaaca tccgctgtat
     6001 cgcgatattg aaaccattgt gcgtgtcaac gcgctggatt ccgaatgggg tgttaacgac
     6061 ctggaagccg tcgttcgcgg tggtgcggac gttgtgcgtc tgccgaaaac cgataccgct
     6121 caggatgttc tggatattga aaaagagatc ctgcgtatag aaaaagcctg tgttcgtgaa
     6181 cccggcagca ccggcctgct ggcggcgatt gaatctccgc tgggcattac ccgcgcagtg
     6241 gaaatcgctc acgcttccga gcgtttgatc ggtatcgccc tcggtgcaga agactatgtg
     6301 cgcaacctgc gtacagaacg ctccccggaa ggaactgaac tgctgttcgc acgctgttcc
     6361 attttgcagg ccgcgcgctc tgcgggtatt caggcgttcg ataccgtcta ttccgacgct
     6421 aacaacgaag ccggattcct gcaagaagcc gcccacatca aacagctggg ctttgacggc
     6481 aaatcgctga tcaacccgcg tcagattgat ctgctgcaca acctctacgc accgacccag
     6541 aaagaagtgg atcacgcccg ccgcgtcgta gaagccgctg aagccgccgc tcgcgaaggc
     6601 ctcggcgtgg tttccctgaa cggtaagatg gtggacggtc cggttatcga tcgcgcccgt
     6661 ctggtgctct cccgtgcaga actttccggc atccgcgaag aataaggcaa tcaaaatgac
     6721 gcagaaaatt gaacaatctc aacgacaaga acgggtagcg gcctggaatc gtcgcactga
     6781 atgcgatctt gccgctttcc agaactcacc aaagcaaacc taccaggctg aaaaagcgcg
     6841 cgatcgcaaa ctgtgcgcca acctggaaga agcgattcgt cgctcaggtt tacaggacgg
     6901 catgacggtt tccttccatc acgctttccg tggcggtgac ctgaccgtca atatggtgat
     6961 ggacgtcatc gcgaagatgg gctttaaaaa cctgaccctg gcgtccagct ccctgagtga
     7021 ttgccatgcg ccgctggtag aacacattcg ccagggcgtg gttacccgca tttatacctc
     7081 tggcctacgc ggtccactgg cggaagagat ctcccgtggt ctgctggcgg aaccggtgca
     7141 gatccactct cacggcggtc gtgtgcatct ggtacagagc ggcgaactga atatcgacgt
     7201 ggctttcctc ggcgtcccgt cctgtgatga attcggtaat gccaacggct acaccggtaa
     7261 agcctgctgc ggctccctcg gctatgcaat ggttgatgcc gacaacgcaa aacaggtcgt
     7321 gatgcttacc gaagaactgc tgccttatcc gcataatccg gcaagcattg agcaagatca
     7381 ggttgatttg atcgtcaaag ttgatcgcgt tggcgatgct gcaaaaatcg gcgctggtac
     7441 tacccgtatg accactaacc cgcgcgaact gcttattgcc cgtagcgctg cggatgtgat
     7501 tgtcaactct ggctacttca aagaaggttt ctccatgcaa accggcaccg gcggcgcttc
     7561 gctggcggta acccgtttcc tggaagacaa aatgcgtagc cgcgatattc gcgccgactt
     7621 cgccctcggc ggtattaccg cgacgatggt tgatctgcac gaaaaaggtc tgatccgcaa
     7681 actgctggat gtgcagagct ttgacagcca tgctgcgcaa tcgctggccc gtaaccccaa
     7741 tcacatcgaa atcagcgcca accagtacgc taactggggt tcgaaaggcg catcggttga
     7801 tcgtctcgac gtggtggtac tgagcgcgct ggaaattgac aggcagttca acgttaacgt
     7861 gctgaccggt tctgacggcg tactgcgtgg cgcttccggt ggtcactgcg ataccgcgat
     7921 tgcctctgcg ctttccatca tcgtcgcgcc actggtacgc ggtcgtattc cgactctggt
     7981 ggataacgtg ctgacctgca tcaccccggg ctccagtgtc gatattctgg tcacagacca
     8041 cggtatcgca gttaacccgg cacgtccgga actagcagaa cgtctgcagg aagcgggcat
     8101 taaagtggtt tccattgagt ggctgcgcga acgtgcgcgt ctgctgaccg gtgaaccaca
     8161 gccgattgaa ttcaccgacc gcgtcgttgc cgttgtgcgt taccgcgatg gctcggtgat
     8221 cgatgttgtg catcaggtga aggaataagc catgcacctg cttcctgaac tcgccagcca
     8281 ccatgcggtg tcaattcccg agctgctcgt cagccgggat gaaaggcaag cacggcaaca
     8341 cgcctggctc aagcaccatc ctgttccact ggtctccttt accgtggttg cgcctgggcc
     8401 gattaaagac agcgaggtca cacgccgaat ttttaatcat ggcgtgacag ccttgcgtgc
     8461 cttagccaca aagcagggct ggcaaattca ggagcaggct gcactggttt ccgccagcgg
     8521 gccggagggc atgttgagca ttgccgcccc ggctcgcgat ctcaagctcg ccaccattga
     8581 gcttgaacat agtcatcctc tcgggcggtt atgggatatc gatgtcctga cgcccgaagg
     8641 cgaaattctc tcccgccgcg actattcact gccgcctcgc cgctgcctgt tgtgcgagca
     8701 aagcgcagcc gtctgcgcgc gtggaaaaac ccatcaactg accgatttac tcaaccgcat
     8761 ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac taaaaccaca aagcttgcga
     8821 cgtcattaat cgatgagtac gccctgctgg gctggcgcgc catgctgact gaagtcaatc
     8881 tgtcaccgaa accaggcctc gtggatcgca ttaactgcgg tgcgcacaaa gatatggcgc
     8941 tggaagattt ccaccgcagc gcgctggcga ttcagggctg gttaccccgt ttcattgaat
     9001 ttggtgcctg tagtgcagaa atggcaccgg aagcggtact ccacggatta cgcccaattg
     9061 gtatggcttg cgaaggtgat atgttccgcg ccactgcggg cgtaaacacg cataaaggca
     9121 gcattttttc tttagggctg ctatgtgcgg caattggccg tttgcttcag ctcaaccagc
     9181 cggttacgcc aacaaccgtt tgttctacgg cggcaagttt ctgccgtggc ctgaccgatc
     9241 gcgaactgcg taccaataat tcacgactga cggcaggtca acggttgtac cagcagcttg
     9301 gccttaccgg cgcacgcggt gaagccgaag cgggttatcc actggtgatc aaccacgcct
     9361 tgccgcatta cctcactctg ctggatcagg ggttagatcc tgaactggca ttgctcgata
     9421 ccttgctcct actgatggct accaacggcg ataccaacgt tgcatcgcgc ggtggcgagg
     9481 ggggcctgcg ctggctacag cgcgaggcgc aaacattatt gcaaaaaggg ggcattcgaa
     9541 cccccgccga tctcgattat ctccggcagt tcgacaggga gtgtatcgaa cgaaatctca
     9601 gtccaggcgg cagtgccgac ctgctgatcc ttacctggtt tttagcacag atttaattat
     9661 ttaagcactt gataaatttg gaaatattaa ttttcggaga acccgtatgt ctttagcaaa
     9721 agataatata tggaaactat tggccccact ggtggtgatg ggtgtcatgt ttcttatccc
     9781 tgtccccgac ggtatgccgc cgcaggcatg gcattacttc gctgtgtttg tggcaatgat
     9841 tgtcggcatg atcctcgagc caattccggc aacagcgatc agttttattg cggttactat
     9901 ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa gaattagctg acccagcgtt
     9961 taatgcgcaa aaacaggcgc tgaaatgggg cctggctggt ttttccagca ccacggtatg
    10021 gctggtattt ggcgcattta tttttgcatt agggtatgaa gtttccgggt taggtcgtcg
    10081 cattgccctt ttcctggtga aattcatgga caaacgcacg ctgacgttgg gttatgcgat
    10141 tgtcattatc gacattctgc tggcaccgtt tacaccgtcc aacaccgcgc gtaccggggg
    10201 tacggttttt ccggtcatta aaaacctgcc gccgttgttt aaatcattcc cgaacgatcc
    10261 gtccgcgcgt cgtattggcg gctatttgat gtggatgatg gtcattagta ccagtctgag
    10321 ttcatccatg tttgtcaccg gtgcggcacc aaacatgctg ggtctggagt tcgtcagcaa
    10381 aattgccggt atccagatta gctggttgca gtggttcctc tgcttcctgc cggttggggt
    10441 tatcttgctt atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg aaatcacaca
    10501 cagtgaagaa gtggcaacct gggcgggtga tgaactaaaa accatgggtg cgctgacacg
    10561 cagagagtgg acgctgattg gccttgtatt gctcagctta ggtttgtggg tatttggcag
    10621 tgaagtcatt aatgctactg cggttggtct gctggcagtt tcgctaatgc tggctctgca
    10681 cgttgtgccg tggaaagaca ttacccgcta taacagcgca tggaacacgc tggtcaacct
    10741 ggcaactctg gttgtgatgg ctaacggcct gactcgttct ggttttattg actggttcgc
    10801 cggtaccatg agtacgcacc tggaaggatt ctcaccaaac gcaacggtga ttgtactggt
    10861 tctggtgttc tactttgcac actacctgtt tgccagcctg tctgcgcaca ccgcaaccat
    10921 gctgccggtt attctggccg tcggtaaagg tattccgggc gtaccaatgg aacaactgtg
    10981 tatcctgctg gtgctgtcta tcggtatcat gggctgtctg acgccgtatg caaccggtcc
    11041 tggggtgatt atttacggct gtggctatgt gaaatcaaaa gattactggc gtcttggcgc
    11101 aatcttcggg gtgatttaca tctctatgtt actgttggtt ggctggccga ttctcgccat
    11161 gtggaactaa tacgttcgcc aaacgctgaa taaatcatca cgcccgccag gtaagcccac
    11221 ctggcgggct tttttatgat ttaatagata gtattcagtg tatgtatgag ttccacaccc
    11281 attatgaaag cattctgccg taacgccgcg ttgctcgcgg tttctctgct tcccttatct
    11341 tctgccaacg ccgtagcgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
    11401 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
    11461 gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac cgtacatggt
    11521 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
    11581 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcacgcgc cagccgaatg
    11641 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
    11701 ccaggagctg gcggacgttc ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc
    11761 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
    11821 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
    11881 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
    11941 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
    12001 aacgctccgc tttctgcaaa ctcattcttg ccacaacctc acccaggtaa ctgtggcaaa
    12061 acctttgtga ttgataaagc gggttattaa tcctctgatt tacaaaacac gctaattcgt
    12121 cttccataac catgccgatt aagaattata cataatgcgc tgcattgagg aattccctga
    12181 atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc
    12241 ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac
    12301 cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa
    12361 cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta
    12421 aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg
    12481 acaatgaaca gccgggttaa attccgcaat cttagagatg gcgaagtgcg tgtgcgcacg
    12541 ctggtgtatc cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta
    12601 ggtgccgcac tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc
    12661 gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac
    12721 ctgctttaat cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc
    12781 ctctttacgc aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt
    12841 attccgtttc gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc
    12901 tgattatggc ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgat
    12961 catattgttc cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct
    13021 gcgctttgtg caccgggtac cagaatcacc ttacggcact cctcttcacg tttttcgaaa
    13081 atctcatatc cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt
    13141 ttcagtaatc ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc
    13201 tgtcccattt taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa accgtgaata
    13261 aatccagcgt agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac
    13321 tggcgcaacg ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg
    13381 gtgctgcctt tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct
    13441 gccgtttgtt caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc
    13501 gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc
    13561 tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accagcacca
    13621 tagactgcaa cgcttgaacc ttgttggatc tgcacatttt ttgctgcctg ccatgccgtt
    13681 ggcagaatat cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac
    13741 ggccccacat tccctttagg gacgcggaca tattccgcct gcccgctagg aacgccacca
    13801 tacaggtgac tataaccaaa caatgccgcg ggagcaggta tctgtttttt attgagcgca
    13861 gcgcctttac ccgcattggt attttcgcag gcggcatatt gctgtaatcg acagaaaaaa
    13921 cagtcgccac aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt
    13981 acgtccttcc cggtttcaac gacttcccca taaattcatg accaaaaata tcgccatgtt
    14041 taacctgggg tattttgcct cgataaagat ggaggtcaga gccacagatc gccgttaccg
    14101 taatacgcag aataatatca tctgcctgtt caatgcccgg atcgggaaca ttttctacct
    14161 gaacgtgatg tgggccgtga taagtcaatg ctttcatgca acctccagag acagttcaaa
    14221 aaagagtcag tctttaaggg tagctatcac agcgggaaaa cgggcggtac cgcaggtttt
    14281 gggagaattc ctggtgttca gtgagaatat attaatcaca gaaaccgttc gtaaggaaaa
    14341 agaaccagac aaatatcagg gataaatatt ataatcatgg cgaatgattt gttcatgatt
    14401 aacagggaga aagggttatg tataaggcaa tcattatgcc agttgatgta tttgaaatgg
    14461 aattgagcga caaagctgtt cgccacgctg aattcctcgc ccaggatgac ggagttattc
    14521 atctacttca cgtactaccc gggtcagcca gcctgagcct gcaccgtttt gccgctgatg
    14581 tgcgtcgttt tgaagagcat ctgcaacatg aagcagaaga acgtctgcaa acgatggtca
    14641 gccacttcac catcgatcct tcccgcatta aacaacatgt ccgttttggt agcgtgcggg
    14701 atgaagtcaa tgagttggca gaagaactgg gggctgatgt tgtagttatc ggttctcgca
    14761 acccatcgat ttcgacccat ctgttaggtt ctaacgcctc gagcgtgatc cgccacgcca
    14821 atctgcctgt actggtcgtt cgctaacaga taaaagtatt tatgccgccc tgcctggaac
    14881 atcccggcag ggctttttaa tgccataaaa aagccgccag gtttgacccc ggcggctaag
    14941 caattgcagg tgaatcttac ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa
    15001 gcactcagag aggctttggc accttcgcca gtggcgatga tgatctgctt gtacggaacc
    15061 gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg tttcacattt cgcatcaatg
    15121 ataatctcgc ccatgcggtt acgttcgact gcgccttcca gccagttggt gttcgggagc
    15181 agaccaatct ggacaaaaat accggccagt tcgatgttgt gaatattgcc gctgacacga
    15241 tctcgatatt ccagaccaac gactttgctg ccgtcgcctt tcacttccgt ggtttgcgca
    15301 ttcagaataa tgtcgacgtt tttcaggctg cgcagtttgt cctgcagaac ctggtcggct
    15361 ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa cgatacccgc caggtcgatt
    15421 gccgcttcca cgccggagtt accgccgccg ataaccgcta cgcgtttgcc tttaaacagc
    15481 gggccgtcgc agtgcgggca gtaggtcacg cctttagtgc gatactgatc ttcgcccgga
    15541 acgttcatgt tgcgccattt tgcacccgtc gccacgataa tgctgcgtgc tttcagtacc
    15601 gcgccagaag ctgtttcaat ctggtgtaga ccaccttcaa ccgctgccgg gatcagtttg
    15661 ctggcgctct ggctgtcgat cacatcaacg tcgtattcat caacgtgaac tttcagtgcg
    15721 cctgccaact tctgcccttc agtcttcggt acagaaatgt agttttcgat atcaacggta
    15781 tcgaggatct gaccaccaaa acgttcgccc atcagaccgg tacggatgcc tttacgtgcg
    15841 gagtaaattg ctgccgctgc acccgccggg ccggaaccaa cgattaatac gtcataagca
    15901 tcacgcttgt tcagctcttc tgccgcacgt ttttccgcgc cagtatcaat tttggcaacg
    15961 atttcagtca acgtcatgcg gccctgacca aactctttcc cgtttacgaa cactgccgga
    16021 acgcccatca cgttgcgatc ggtgatttcg ttctggaagg tgccgccgtc aattgcagtg
    16081 tgcttgatgc gcgggttcaa tacgctcatc aggttcagcg cctgcaccac gtccgggcag
    16141 ttatggcaag agagcgagta ataggtttcg aattcaaaat caccgtcaat atggcgaatc
    16201 tgctccagca gagactgcgc ttctttcgac ggatgaccac cggtccacag caacgccagt
    16261 accagcgagg tgaactcgtg gcccagcggg gagcctgcaa aacgtggtcc ctggttggaa
    16321 cctgggttgg tgatcaggaa agacggctta cgcactggca agctgttatc ttctttaaag
    16381 gtgactttgt ctgacagttc tgcgatttca gccaacagtt ccttgatttc tgccgattta
    16441 gcgctgtcat ccagcgtggc aattaactca acaggcttgg tcaatttctc aaggtaagcc
    16501 ttgagttgag ttttcatatt tgtgtcgagc atgtttatct cctgggcttt aaacatcatc
    16561 atgcaagcgg cctctgcggc tacctggatg cagcttgcat catggtgcgg ggaagaaaat
    16621 cgggtgccgc agcacccgaa gaattaggtg aatttccgaa gtatttcgcg tcagagcaag
    16681 gcgactagcc tgtgaatccc cgggagctta cacaagtaag tgaccggggt gagcgggcga
    16741 cgccaacgcc gctatggcgt gaaagacgaa ggaaatttag attttaccaa ccaggtccag
    16801 agacggagcc agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg
    16861 agaagctacg tactgagctg ctttgatttt acgcagcaga tcagacgcgt cacggccaat
    16921 gccttcagcg gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc
    16981 acggtcagcc agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt
    17041 cgggtcgccg atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc
    17101 tttgtgggtg aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag
    17161 ttcttcgtag tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc
    17221 cgggtagaag aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa
    17281 ttcgccgttt ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat
    17341 ctatacttcc tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta
    17401 atgcgtttcc attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt
    17461 gaactttgca ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt
    17521 aatctgaact accctttggt atcttcagcg cctgcctgca ggctgaggtg ttgcccttac
    17581 aaatgcaaca acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta
    17641 agctcttacc tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa
    17701 agaacaaatt aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg
    17761 cagaggaact tccttcccca gtaaaagcga ttgaaaaaca tggcattaca atcatcaaaa
    17821 cgttcgatgc ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca
    17881 ccatctacct gactcccgat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag
    17941 gtgaaaacct gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa
    18001 tgtggcaacg gatggaacaa tcccactggc tcctcgacgg taaaaaagat gcgccggtca
    18061 ttgtctacgt cttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc
    18121 gcccgtgggt agattctggc aaagtgcaat taagaacatt gttggttggg gttatcaagc
    18181 cagaaagccc ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc
    18241 aacaatatga agcctctggt tgcaagctta agctaaacgt gcctgcaaac gtaagtacag
    18301 agcaaatgaa agtgttaagt gacaatgaga aactgatgga cgatctgggg gcaaatgtca
    18361 cgccggctat ctattacatg agtaaggaaa atacgctgca acaggccgtg gggttgcccg
    18421 atcagaaaac gcttaatatc attatgggaa ataaataaaa ggtaattcat ggatggttga
    18481 actatatccc tgcttttcaa ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt
    18541 ggctgtgcta ttttagaaaa acttaatcct gagttaagca aaaaaacata atcaataaaa
    18601 atacatacgc aaaaaacatt gattaagtga atataccatg gaagaaaaat ataaaccgga
    18661 gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt
    18721 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
    18781 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
    18841 tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga
    18901 gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
    18961 aaactttatt atctacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
    19021 ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
    19081 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
    19141 gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag
    19201 taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
    19261 cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa
    19321 gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaataa ttaatagcat
    19381 tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactacc accgtgactt
    19441 tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc
    19501 ctataataaa tcttctttaa ataatttggt gttctcccga tttattgacc gtctaaatga
    19561 gagtttttga tatataacta cagacagcta ctataacttt catctattta ttcacagctc
    19621 gaatgaaaaa taaaacactc tgctttacag tgtttacttt ataattaaca gtcttatctc
    19681 actgttcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt
    19741 ccactcaata ttctggaagc tgcacgagag cgtattacct agacactcaa caccctaccc
    19801 cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc
    19861 gaacttgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggc
//
LOCUS       DAJTRQ010000070        19554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000070 DAJTRQ010000000
VERSION     DAJTRQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            18..209
                     /locus_tag="OO332_002518"
     CDS             18..209
                     /locus_tag="OO332_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5942956.1"
                     /translation="MVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFS
                     TVFKQRFGMTPGEYRRKFR"
     gene            complement(381..710)
                     /locus_tag="OO332_002519"
     CDS             complement(381..710)
                     /locus_tag="OO332_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5942957.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(715..900)
                     /locus_tag="OO332_002520"
     CDS             complement(715..900)
                     /locus_tag="OO332_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5942958.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(897..3536)
                     /locus_tag="OO332_002521"
     CDS             complement(897..3536)
                     /locus_tag="OO332_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5942959.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3744..4733
                     /gene="ldhA"
                     /locus_tag="OO332_002522"
     CDS             3744..4733
                     /gene="ldhA"
                     /locus_tag="OO332_002522"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5942960.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4844..5191
                     /gene="hslJ"
                     /locus_tag="OO332_002523"
     CDS             4844..5191
                     /gene="hslJ"
                     /locus_tag="OO332_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5942961.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5188..5454)
                     /locus_tag="OO332_002524"
     CDS             complement(5188..5454)
                     /locus_tag="OO332_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5942962.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5728..9252
                     /gene="nifJ"
                     /locus_tag="OO332_002525"
     CDS             5728..9252
                     /gene="nifJ"
                     /locus_tag="OO332_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5942963.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9619..10748
                     /gene="ompN"
                     /locus_tag="OO332_002526"
                     /pseudo
     CDS             9619..10748
                     /gene="ompN"
                     /locus_tag="OO332_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            10889..11323
                     /gene="uspF"
                     /locus_tag="OO332_002527"
     CDS             10889..11323
                     /gene="uspF"
                     /locus_tag="OO332_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5942964.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11499..12434
                     /gene="ttcA"
                     /locus_tag="OO332_002528"
     CDS             11499..12434
                     /gene="ttcA"
                     /locus_tag="OO332_002528"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5942965.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12563..13930)
                     /gene="dbpA"
                     /locus_tag="OO332_002529"
     CDS             complement(12563..13930)
                     /gene="dbpA"
                     /locus_tag="OO332_002529"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5942966.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14408..15391)
                     /gene="zntB"
                     /locus_tag="OO332_002530"
     CDS             complement(14408..15391)
                     /gene="zntB"
                     /locus_tag="OO332_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5942967.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15646..16878
                     /gene="dgcM"
                     /locus_tag="OO332_002531"
                     /pseudo
     CDS             15646..16878
                     /gene="dgcM"
                     /locus_tag="OO332_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(16899..17462)
                     /gene="smrA"
                     /locus_tag="OO332_002532"
     CDS             complement(16899..17462)
                     /gene="smrA"
                     /locus_tag="OO332_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5942968.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17716..19170)
                     /locus_tag="OO332_002533"
     CDS             complement(17716..19170)
                     /locus_tag="OO332_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5942969.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19167..>19554)
                     /locus_tag="OO332_002534"
     CDS             complement(19167..>19554)
                     /locus_tag="OO332_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5942970.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5138 a         4962 c         4601 g         4853 t
ORIGIN      
        1 gtttgccgat ataaggtttg gtagtcgcgc aatatattcg taaccgtcgt ctcgattttt
       61 gtgcagatgc gattcgccat gccgcagatg atgaaaaact ggcaggcatc ggctttcatt
      121 ggggattttc tgaccagagt catttctcaa cggtatttaa gcaacgcttt gggatgacgc
      181 caggcgagta tcgccgtaaa ttccgctaac taattggcgt tgcatgtact gcaacgccaa
      241 ttagttaaaa cagatatttg actatgcatt tttcagggcg aaaggcaatt tgacaggagt
      301 ttttgaatat tcaccttgtt tcaggaaaat agatcaatcg ctttctcctg tagcgttatc
      361 ccgatattgc ggttttgcta ttaaaacttt cgcacccatt taccgtttat cccttgtaca
      421 tattcccccg gtttggcccg agctacgagc ttttgcccgg cgagtttcgc gatatcatct
      481 acagacacat tattttgctt tgcccgttgt tggtaactcg ctttacgttc ggcattaatt
      541 tcggttacta atttctctgt ttcagcatcc gttttcagcg caaccagata accataaaat
      601 gtttcaccta cccgcccctg agttctggct tcatccagag tcaatgccat tacattgctg
      661 cttaccagcc caacaagaaa cgcacaaaga agtaatgttt tcttcatcat cgcctcagaa
      721 aagatcgcta cgagtttcaa gcagctcttc gacatctttg tctgccttga tgatgatctc
      781 atgctcaatt ttaacgttca tattgatagt gatcggttcc ttaggtgcag cgacttcaat
      841 gcgaggcgta cagccaacca gcataaaaga tgacgtcaac gcagccagta aaattttcat
      901 tgtttttcct cacactcttt tccttgcgga cagtcatttc ccggcagacg tgcgttctgc
      961 tccaaccatg cctggagatt atcgccaaag cgtaaactgc gccacagcgt aaaaatattc
     1021 tcttcatgat ggtaattaag gtttaccgta ccgcttttac catcaacgcg actggtacct
     1081 gtaatgttgg cctgcatggt taataaaccg agattatcta agttaatttt tgtcgatgac
     1141 cggctaattt ccatatagcg caaccagtta attgctgaac ccgcagtcat attgtctttc
     1201 accaccgcat ctgcggtatc tttatccagc cgcaaggtca ttggcccgct atttgccagc
     1261 caaccatctt tcactatcca tttttcgttg tttagccata acggcaatgc accactaaat
     1321 gccccggaca tggcgaattg tttcggattg acggcgctaa ccagttcgct ggatgagaga
     1381 ttattcagcc gcaacagcgc cgggtcatgt tgcggcatac gtaattgttt catcagcacg
     1441 ttaccgccta acacatcgac gctaacatca gtcaacagca agggttcttc ttcagtccac
     1501 ggataacgcc cttgcaaatc agccgtaata tttttcgctg tcaccagatt aatcacttcg
     1561 gcaattcgca acgtaacggg gccgcgagtc cccagatgcc aggctccatc ggcaaaacgg
     1621 aaaggcagga caaaatcgac accgttaacc tgattatctg gcatccaggc actaccgcct
     1681 ttcaacacgc cgtgccctcc cgcgcggaat ccttgttcag gcgcagcaga aaatgcaacc
     1741 tgagcataca gttcaccatc gcgtaagttc atcttccagt cgggtggcac cagcggctgg
     1801 aatacagtca gtgattgttt tggccaccag gcgttaccgc gcagacgaat accgtcccag
     1861 cgaccattta cccgaaccgg gccaatctca ccaacatgta aatcgccttt aaagaggaaa
     1921 taggtcggat cgcgcccatc gacgctaaat tttaaggttg atggcggtaa cacactgccg
     1981 ccagtgaaca gcgtttgccc ggcgtccagt gacagcgcgc cgctgaagga gggatgctgt
     2041 gcgtcacgta cccagacgac gggcttgtcg agaattaatc gcggcttttc taccgtcatc
     2101 gtaccgtatt gtaactggtc gaaaccggtg gagagatcgg tcagcgtaat cgtgctgtca
     2161 tgccactcac cttttcctgc gacatcccag gtggcattca tcggtgtaaa actccctttt
     2221 ccccagtagc gccactgcca gcggccagcg tcagggagaa aatcattcgc cagcccatcc
     2281 atatgcagca cgaaatcgcc cagttcattt tcatgcgcct gcaaaatggc ctgcaaacgt
     2341 ccgtcaacac cacgttgggt gacttttaca cccgctaaag gccagcggat ttcatcgatg
     2401 tccagcgaat cgatgactct tccctttgaa cgaagtaacg cgcctggctc aaaggtcagc
     2461 gttgggtcag acagacttcc acttagctgc gcaggtaaac gggcatataa aatgaggtcc
     2521 gcctgtttcg cttcaccggt cagctgcaga ggcagttgac tgttatccat gcttaatttt
     2581 cctgggccaa aattaagcac cgcgttgccc ttacccgctt gcccctgggt cagcacactc
     2641 agtcgtccgc tgaccagagc gttctcaagc cctgcctgcc agttgtcaac tttgacaccc
     2701 agtcggccac tcaaaggaaa acctgcatac agccagctcc agcgaccatc gcttacggtc
     2761 aattgttgac gagtaatttg ccacggcaaa tcgagcaacg gatcgccgtt atcccgtgcc
     2821 agcacgatca attgcccgct attttcctgc cagtccagct cggcatccac cagtgacggt
     2881 tcctgcggca agtttaacgt cgcagtagca tgcccactta caggaagtcc atccggcacg
     2941 agcggcatag taaattcccc caccagttta accggcggct gattttcaaa cgcgacgaca
     3001 tccagttcgc tgactgtaag ttgttgccct ttcagctggc cttgaaattt aactttttcg
     3061 ccctgataac gcagttgctg gatatcagag gttaatgcga gagagagttt tccctgccat
     3121 tcctgccagg gagaaaaaat cagtttatcg atattgatcc aggtgttagg cagcatggac
     3181 tgccactgcg cgagggtttt tggagcagct ggcgattgct ccgtctgcgg caatttcgcc
     3241 agacaagcag aatcaagttc taccgtgccg acgttcaata accagcggct gggatgtgaa
     3301 aggctggcgt tggtgatatg cgcaagctga caatctccca ccagataacg gagatcggga
     3361 atgattaaac ctttacgcgt aatgcgtggg ctttcatcta atgcaatacg tgtcccgagc
     3421 ggtagccaga tgtccgccag cgtgggaacc cacagcccga gcgtcatcag cagcgtcaac
     3481 ggcacaagaa taatcagtaa taacagcgcg agaacggctt tatatttacc cagcatgggt
     3541 agttaatatc ctgatttcgc gaaaaattaa gcattcaata cgggtattgt ggcatgttta
     3601 accgttcagt tgaaggttgc gcctacacta agcatagttg ttgatgaatt tttcaatatc
     3661 gccatggctt tcaattatat ttgaaatttt gtaaaatatt tttagcagct taaatgtgat
     3721 tcaacatcac tggagaaagt cttatgaaac tcgccgttta tagcacaaaa cagtacgaca
     3781 agaagtacct gcaacaggtg aacgagtcct ttggctttga gctggaattt tttgactttc
     3841 tgctgacgga aaaaaccgct aaaactgcca atggctgcga agcggtatgt attttcgtaa
     3901 acgatgacgg cagccgcccg gtgctggaag agctgaaaaa gcacggcgtt aaatatatcg
     3961 ccctgcgctg tgccggtttc aataacgtcg accttgatgc ggcaaaagaa ctggggctga
     4021 aagtagtccg tgttccagcc tatgatccag aggccgttgc tgaacacgcc atcggtatga
     4081 tgatgacgct gaaccgccgt attcaccgcg cgtatcagcg tacccgtgac gctaacttct
     4141 ctctggaagg tctgaccggc tttactatgt atggcaaaac ggcaggcgtt atcggtaccg
     4201 gtaaaatcgg tgtggcgatg ctgcgcattc tgaaaggttt tggtatgcgt ctgctggcgt
     4261 tcgatccgta tccaagtgca gcggcgctgg aactcggtgt ggagtatgtc gatctgccaa
     4321 ccctgttctc tgaatcagac gttatctctc tgcactgccc gctgacaccg gaaaactacc
     4381 atctgttgaa cgaagccgcc ttcgatcaga tgaaaaatgg cgtgatgatc gtcaatacca
     4441 gtcgcggtgc attgattgat tctcaggcgg caattgaagc gctgaaaaat cagaaaattg
     4501 gttcgttggg tatggacgtc tatgagaacg aacgcgatct gttctttgaa gataaatcca
     4561 acgacgtgat ccaggatgac gtattccgtc gcctgtctgc ctgccacaac gtgctgttta
     4621 ccgggcacca ggcattcctg acagcagaag ctctgaccag tatttctcag actacgctgc
     4681 aaaacttaag caatctggaa aaaggtgaaa cctgcccgaa cgaactggtt taatcttgcc
     4741 gctcccctgc aatccagggg agctgattca gataatcccc aatgaccttt cattctctat
     4801 tcttaaaata gtcctgagtc agaaactgta attgagaacc acaatgaaga aagtagccgc
     4861 gtttgttgcg ctaagcctgc tgatggcggg atgtgtaagt aatgacaaaa ttgctgttac
     4921 gccagaaatc agctttggtg aaaaaatgat gatttccggc agcatgtgta accgctttag
     4981 cggtgaaggc aaactgtcta atggtgaact gacagccaaa gggctggcaa tgacccgtat
     5041 gatgtgcgct aacccgcagc ttaatgaact cgataacacc attagcgaaa tgctgaaaga
     5101 aggtgcacaa gtggatctga ccgcgaacca gttaacgctg gcgaccgcga aacagacatt
     5161 aacttataag ctggcggatt taatgaatta atagctgcca cagctcccgg cggcaagtga
     5221 ctgttcgcta cagcgtttgc cgttgggtaa tgcacacatc ccaatcgccg taccatccag
     5281 ttgacgggca acagaaagcg aaccgccgat cattgcacaa tttgcttctc cactactgga
     5341 catcgacgct tttaaacttg gcgctacgtg cgccgcagtc gcctgctgaa caggctcact
     5401 actacacgcc gacaacaata aagcggcaca ccctacccaa aacgctgctc gcatctcttc
     5461 ttcctctgat cttcaagcca aacgacaccg ccataaataa taggcagcac agagggcggc
     5521 gtcgagagct gtcctgcgcg ttgccccgca tttttacttt tttatggcta tttttttgcc
     5581 ctctgtttga tcaaaacatt cattactctg atgtggggga cacaaaagcg aaaatgcaga
     5641 agaaagccat ttgctaaaat tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc
     5701 ctcatttgcg caatgtaagg gtgtcatatg attactattg acggtaatgg cgcggttgct
     5761 tcggtggcgt ttcgcaccag tgaagttatc gccatctacc ctattacccc cagttccacg
     5821 atggcagaac aggctgatgc ctgggccgga aacggcttga agaacgtttg gggagacaca
     5881 ccacgcgtgg ttgaaatgca gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg
     5941 cagacgggtg ccctttcaac atcgtttacg tcatcgcagg gtttgctgct gatgatcccg
     6001 acgctgtaca aactggcagg cgaactgacg ccatttgtcc tgcatgtagc ggcacgtacc
     6061 gttgccacac atgcactttc tatttttggc gatcattccg acgttatggc ggtgcgccag
     6121 acgggttgcg cgatgttgtg tgcggctaac gtccaggaag cgcaagactt tgctctcatt
     6181 tcacacatcg caacgctgaa gagccgcgtg ccatttattc atttctttga tggttttcgc
     6241 acgtcccacg aaatcaataa aattgtcccg ctggccgatg acactattct tgatctcatg
     6301 ccgcaggccg aaattgatgc tcatcgcgcc cgggcgctca acccggaaca tccggtgatc
     6361 cgcggtacgt ccgccaatcc tgacacttat ttccagtctc gcgaagccac caacccctgg
     6421 tacaacgcgg tctatgacca tgttgaacag gcgatgaatg atttcgctgc cgcgacaggt
     6481 cgtcagtatc agccgtttga gtattacggg catccgcaag cggaacgggt gattatcctg
     6541 atgggctctg ccattggcac ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa
     6601 aaagttggcg tgctgaaagt tcgtctgtac cgtcccttct ccgccaaaca cttgctgcaa
     6661 gctctgccgg gatccgtacg cagcgtggcg gtactggaca gaaccaaaga acccggtgcc
     6721 caggcagaac cgctctatct ggatgtgatg accgcactgg caggagcctt taataatggt
     6781 gagcgcgaaa ccctgccccg tgtcattggt gggcgctatg gtctttcatc caaagaattt
     6841 ggcccggact gtgtactggc ggtatttgcc gagctcaacg cggctaaacc taaagcacgc
     6901 tttacggttg gtatttacga tgatgtgacc aatctgtcac tgccgttgcc ggaaaacact
     6961 ctgccaaact cggcgaaact tgaagccttg ttttatggcc ttggtagtga tggcagcgtt
     7021 tccgcaacca aaaacaatat caagattatc ggtaattcca cgccgtggta cgcacagggc
     7081 tattttgttt acgactccaa aaaagcgggc ggcctgacgg tttctcacct tcgcgtgagc
     7141 gaacagccga ttcgttccgc ttatctcatt tcccaggctg attttgttgg ctgccaccag
     7201 ttgcagttta tcgataaata tcagatggct gagcgtttaa aacctagcgg cattttcctg
     7261 cttaatacgc cgtacagcgc agatgaagtg tggtcgcgct tgccgcagga agttcaggcc
     7321 gtgttaaacc agaaaaaagc gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa
     7381 tgtggcctgg cggcccgtat taataccgtc atgcagatgg cttttttcca tctgacgcaa
     7441 attctgcctg gcgatagcgc cctcgcagaa ttgcagggtg cgattgccaa aagttacagt
     7501 agcaaaggtc aggatctggt ggagcgcaac tggcaggctc tggcgctggc gcgtgaatcc
     7561 gtagaagaag ttccgttgca accggtaaat ccgcacagcg ccaatcgacc gccagtggtt
     7621 tccgatgccg cccctgattt cgtgaaaacc gtaaccgcgg cgatgctcgc cgggcttggt
     7681 gatgccctcc ccgtttcggc gctgccgcca gacggcacct ggccgatggg caccactcgc
     7741 tgggaaaaac gcaatatcgc cgaagagatc cccatctgga aagaggaact ctgtacccaa
     7801 tgtaaccact gcgtcgccgc ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct
     7861 gaagcgatgg aaaacgcccc tgccagcctg cattcgctag atgtgaaatc gcgtgatatg
     7921 cgcgggcaga aatatgtctt gcaggtggca ccggaagatt gcaccggttg taacctgtgc
     7981 gtcgaagttt gcccggcgaa agaccgtcag aatccagaga ttaaagccat caatatgatg
     8041 tctcgccagg aacatgtcga agaagagaaa atcaattacg atttcttcct caacctgcca
     8101 gaaatcgacc gtagcaaact ggaacgtatt gatattcgta catcgcagct gattacaccg
     8161 ctgtttgaat attcaggtgc ttgccccggt tgtggcgaga cgccgtatat taaattgctg
     8221 actcagctct atggtgaccg gatgttgatc gctaacgcca ccggctgttc ttcaatttat
     8281 ggcggtaacc tgccctctac accgtatacc accgatgcca acggtcgtgg gccggcatgg
     8341 gcgaactctc tatttgaaga taatgccgaa tttggccttg gtttccgcct gacggtcgat
     8401 caacaccgtg tccgcgtgct gcgtctgctg gatcaatttg ccgataaaat cccggcggaa
     8461 ttactgacgg cgttgaaatc agacgctacg ccagaggttc gtcgagaaca ggtcgcagct
     8521 ttacgccagc aactcaacga tgttgccgaa gcacatgaac tgctgcgtga cgcagatgca
     8581 ctggtggaaa aatcaatctg gctgattggt ggcgacggct gggcttacga tatcggcttt
     8641 ggcggtctgg atcatgtatt gagtttgacg gaaaacgtca acattctggt gctggatacg
     8701 caatgctatt ccaacaccgg tggtcaggcg tcgaaagcga caccgctggg tgcagtaact
     8761 aagttcggcg agcacggcaa acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg
     8821 tacggtcatg tttatgtggc gcagatttct ctcggcgcgc agctgaacca gacggtgaaa
     8881 gcgattcagg aagcggaagc gtatccggga ccatcgctga tcattgctta tagcccgtgt
     8941 gaagagcatg gttacgatct ggctctcagc cacgaccaga tgcgccaact cactgctacc
     9001 ggcttctggc cgctatatcg ctttgatccg cgtcgtgccg atgaaggcaa actgccgctg
     9061 gcgctggatt cacgcccgcc gtcagaagca ctggaagaaa cgttacttca cgagcagcgt
     9121 ttccgtcgtc tgaattcgca gcagccagaa gtggcagaac agttatggaa agatgctgca
     9181 gctgatttgc aaaaacgcta tgacttccta gcacaaatgg ccggaaaagc ggaaaaaagc
     9241 aacaccgatt aaatgctctg gataaggatt atccaattct aaaaaaaaag cccggacgac
     9301 tgttcgggct tgtcttttta tatgttggaa aatcagtggc aatgcaatgg cccaacagaa
     9361 aataaaatct gtgacaataa aggcatataa cccgcgcaga ataacgtata gaaaattaaa
     9421 agtataattt ttattttgta tgtattatct tcgatacatt caccagactt attatcattt
     9481 cagaggaatt atttgattaa ggttttactt aaggcgtaac aaatgatatt tatcggcaaa
     9541 ctgaacttct cctttaggat gttttcactc cccgaaatgg ggataaaagg caaataaaat
     9601 aactaaagga tttattcaat gaaaagaaaa gtactggcac ttttaatccc tgccctgctc
     9661 gccgcaggtg ctgcacatgc agccgaagtt tataataaag acggcaacaa attagatctg
     9721 tatggcaaag ttgatggcct gcattatttt tctgataatt cagcgaaaga tggcgaccag
     9781 agctatgcgc gtctgggttt taaaggcgaa acccaaatta acgatcaact tactggctac
     9841 ggtcaatggg aatacaatat tcaggcaaac aacactgaat cttcaaaaaa ccagtcatgg
     9901 acccgtctgg catttgccgg actgaaattt gcagattacg gttctttcga ttacggacgt
     9961 aattatggcg taatgtacga catcgaaggc tggaccgata tgctgcctaa atttggcggt
    10021 gactcttata ccaatgcaga caactttatg actggtcgag ccaatggcgt cgcgacttat
    10081 cgtaatactg atttcttcgg tctggtaaat ggtctgaact tcgcggtgca gtatcaaggt
    10141 aacaacgaag gagccagtaa tggtcaggaa ggcaccaata acgaacgcga tgttcgtcat
    10201 gaaaacggtg acggctgggg tctttccaca acatatgatt taggcatggg ctttagcgct
    10261 ggtgcggcat acacctcttc tgaccgcacc aatgaccagg ttaaccatac tgcggcgggt
    10321 ggtgataaag cagacgcgtg gactgctggg ctaaaatacg atgctaacaa tatttaccta
    10381 gcaaccatgt attcagaaac gcgtaatatg accccgtttg gcgacagcga ttatgcagtt
    10441 gcgaacaaaa cccagaactt tgaagttact agtaccagtt tgattttggt ctgcgtccgg
    10501 cagtctcttt cctgatgtct aaaggccgtg accttcacgc tgctggtggt gcagacaacc
    10561 cggcaggtgt tgatgataaa gatctggtta aatacgccga tgttggcgcg acttactatt
    10621 tcaataaaaa catgtccacc tatgttgact ataaaatcaa cctgttggat gaagatgaca
    10681 gtttctacgc tgccaacggc atctctaccg atgatattgt cgctttaggt ctggtttatc
    10741 agttctaaat cctcctgccc gctgttatgg cgggcttttt ctgcttattc ttcctccttt
    10801 gatctaaatt aaaaatgtga actccgtcat tacacaaaaa gtgtcatctg gcgttacact
    10861 ttatgcggat actaaaacag gaggttttat gaacagaacg attcttgtcc ctatcgatat
    10921 ttcagattca gaattaactc aacgcgtgat tagccacgtt gaggcagagg caaagattga
    10981 tgatgcagag gttcatttcc tgacggtaat accttcactg ccctactatg cctctctggg
    11041 tttagcgtat tccgcagaat taccggcaat ggatgacctg aaagcggaag ccaaatcgca
    11101 actggaagag atcattaaaa aatttaaact gccaaccgac agagtgcatg tccatgttga
    11161 ggaaggctcg cccaaagacc gcattctgga attggcgaag aagatccccg ctcatatgat
    11221 catcattgct tcccatcgac cggatatcac cacttatctg ctcggttcca acgccgcagc
    11281 tgtagtgcgt cacgcagagt gctccgtgct ggttgtgcgc tgacactaac gcccgcacat
    11341 tgctgcgggc tttttgattc atttcgcaaa tgtgctgaca ttttcccctc taatccgtac
    11401 catacacgcc acagttttta tatcagattt cttatgctga gcgttccggc atgacgcata
    11461 ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat caagaaatta
    11521 gtaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt aacgtgggcg
    11581 aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt tgcctctccg
    11641 ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa agcgcgccaa
    11701 tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc ccggaacacg
    11761 ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgttgaa gagaacactt
    11821 acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg tgttctcggc
    11881 tacgtcgcgg tatcctttat cgcaccgcaa cggaactggg gacgacgaag atcgcgttgg
    11941 gtcaccatcg tgacgatatc ctgcaaacgt tgttcttaaa tatgttctac ggcggcaaga
    12001 tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt attcgtccac
    12061 tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg ttcccgatta
    12121 ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt gctgacatgt
    12181 tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc gcgatgcaga
    12241 atgtggtgcc gtcgcatctg tgcgatacca acctgttcga tttcaaaggc attacccacg
    12301 gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc ccactacaac
    12361 cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacgg ctgaatgtgg
    12421 ttgaagtgaa ataaccagga tagcgcccga tgcgcaagcg tatcgggcta ctcttatgga
    12481 ggccggataa gacgcggcca gcgtcgcatc cggcaatccc gaataagatg tttactcttg
    12541 cacccggcaa ttcaacattt cattatttta ataaccgcac ccggcacgtt tttcctttaa
    12601 tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac agcctgacgg accgcgacat
    12661 agacatgcgc cggatgcacg gcgattttgc caatatctgc gccatcaagc ccgatatctc
    12721 ctgtcagtgc acctaataca tcacccgggc gcattttggc ttttttcccg ccatcgatac
    12781 acaacgttgc catttctgct tccagcgtca caatggaact attagctggc ggcgtttgcc
    12841 agttaagttt tatctgcaac atgtcagaaa tgatattggc ccgctgtgct tcttccggag
    12901 cacagaaact gatcgccaga ccgctatttc ctgcacgagc tgtacgaccg atgcgatgta
    12961 catgaacttc agggtcccac gccagctcaa agttcaccac cagctcaagc gatttaatat
    13021 ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg ggcgctaccg ttagcaaaac
    13081 gtaccagggt ctgatcgcgt tgctccagat cgccgtgtaa cgacaatgca ctttgctcta
    13141 cttcattcag cgcgtcgcag acagcctggc aatctttttt ggtattgcaa aacaccacac
    13201 aagaggatgg ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttactgg
    13261 atgtctcata aaattgttgt tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg
    13321 ccaaaggatc gcgttgcact cgtccgctga ttgcggcgat ggcttccggc caggttgccg
    13381 aaaacagaag cgtctgtcga gatgcaggcg caaaacggat gacatcatca atggcatcgc
    13441 taaatcccat atccagcatg cggtcggcct catccatcac caaagtattc agcgcatcca
    13501 gtgataccgt gcctttttgc aagtgatcca gcaaacgacc cggcgttgcc acgataatat
    13561 gcggcgcatg ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca
    13621 aaattttggt atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg
    13681 ccagttcacg cgtaggacac agcactaaag ccagggtttg aaatagcgac gcatcaattt
    13741 gctgtaacaa gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc
    13801 gaacatcttt tcctgcaagg atcgccggaa gcgcggcggc ctgcaccggc gtcatggtta
    13861 aataacccaa ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa
    13921 aagcggtcac aatctattct cgtggtcatc gacgcaaagt tagcaggcgc gtatcctcgc
    13981 agatctacgc tcacgatgcg acaatttaat cggttcttca tcgggtggtg ggtcaggcat
    14041 gggttgcggg cgagggatcg gatcgggcac tggaacagga tcgccaggaa tcggttcagg
    14101 gacaggaatt tgcaaataaa taagtgtcgt catatttccc tccggtcatt gggtggactc
    14161 ttaaagggta gacgctgata aataacaggc aaaaaaaagc cgactcatca aagtcggcgt
    14221 cgtacgaatc aattgtgcta tgcagtaatt caaaaaagga agtaagacaa tatggagcgc
    14281 aacgcccatc gcttgacgtt gcattcacct gcaagggaga tattgccctg aatgggtaga
    14341 gagtttattg acttcgctca aactttgcgg cgtttttgta tacagacagc cgaaaaaatt
    14401 gcttttgtta caaccattta ctacggtgca accataaagc aacaccacca ataagaacaa
    14461 ctaacagaat acaaaaaatt gaaaatccga attgccaccc gccgccaggg atcccaccaa
    14521 ggttgacgcc aaataacccg gtcagaaagg tactgggtaa aaagaccatt gccatcaacg
    14581 acattgtata ggtacgacga gctaaatttt cctgcatcac ctgagcgatt tcatccgcca
    14641 tcacgccagt ccgtgctata caggcgtcga tttcgtcaag gccgcgccca aggcgatcgg
    14701 caatatcctg catccgacgg cgttggtcat cgctcatcca cggcaaacgt tcactggcaa
    14761 gacgagcata aacatcacgt tgcggtgcca tatagcgacg catcacaatt aattgtttgc
    14821 gcagcagagc caggaatcca cgcggtggaa tttgctgatc aaggagatta tcttcaaggt
    14881 cgataatttt atcgtgcagc tgctcgataa attcactgga atgatcggtc aacgcatcgc
    14941 acacatccac cagccatccc ccgcaatcgg tcggacccgt gccctcttcc agatcgctca
    15001 ccacatcgtc cagcgccagc actttgcgtt gtcgggtcga aacaattaac cgcccgtcca
    15061 tatatacacg catggcgacc agttgatcgg ggcgttcatc ggtgctgccg tttatacagc
    15121 gcaatgtaat cagcgtgcct tcaccgaaac ggctgactcg gggccgtgtg ctctcgcccg
    15181 ccagcgcatc acgtacgtta ttgggaagca gcggtgttgt cgccagccat tgggcgctat
    15241 catggtgtac ataattaagg tggagccagc agggatgcgc ttcatcaatc acatctgtat
    15301 tttccagcgg tttaacgccg cctctaccat ccagcatcca ggcaaatact gcatccggga
    15361 cattaacgtc cgatccctta atcgcttcca cagtgcctcc atcatcaacg cattattttg
    15421 tagtctagcc ttctggccct gttacgcaac atctcatcac cccattaccc tgaaatgatt
    15481 aataaaattc tgtctaaatt gaatacaaaa agcaaaatgc ttttccgtat acaaaccgtg
    15541 tgaagtgtta aatagcgtct atcattatca gaattatctg atcatatgac gtggcttttt
    15601 tgcgatcgga tagcaacaaa aattgataaa aataacggga tctcaatgat tacgcacaac
    15661 ttcaataccc tggacttact caccagtcct gtctggattg tttcgccctt tgaggaacag
    15721 ttaatttatg ccaatagcgc ggcgcgactg ttgatgcaag acctcacgtt tagtcagcta
    15781 cgaaccggac cctattccgt ctcctcacaa aaagaactgc cgaaatacct ctccgatctg
    15841 caaaaccaac acgatattat cgaaatcctc actgttcagc gtaaagaaga ggaaacagca
    15901 ttaagctgtc ggcttgtttt gcgagagctg acagaaacag aaccggtgat tattttcgaa
    15961 ggtatcgaag cgccggcaac gctgggttta aaagccagtc gctcggcaaa ttatcagcgc
    16021 aaaaaacaag gtttttatgc gcgctttttt ctgactaact ctgcaccaat gttgttgatt
    16081 gacccgtcac gagatggaca aatcgtcgat gctaacctcg ccgcgctcaa tttctatggt
    16141 tataaccatg aaacgatgtg ccagaaacat acctaggaaa taaatatgct tgggcgtcgc
    16201 gtcatgccta tcatgcatga aatttcgcat ttacccggcg gtcataaccc tttgaatttt
    16261 gttcataaac tggcggatgg ttctactcgt catgtgcaga cctatgccgg accgattgaa
    16321 atttatggcg acaagctcat gttatgtatt gtgcatgata ttactgagca aaaacggctg
    16381 gaggagcagc tggaacatgc tgctcaccat gacgcgatga ccggattact gaatcggcga
    16441 cagttttatc acattacgga accaggccaa atgcagcatc tcgccatcgc tcaggattac
    16501 agcttgttgc tcatcgacac cgatcgtttt aaacacatta acgatctcta tgggcattct
    16561 aaaggtgatg aggtgttatg cgccctcgcc cgccccctcg aaagttgcgc tcgcaaaggc
    16621 gatttggtgt ttcgttgggg aggcgaagag tttgtcttat tgctaccaag aaccccactg
    16681 gataccgcgc tttcgctggc tgaaactatc cgcgtaagcg tggcaaaagt gagtatttcg
    16741 ggcttaccac gctttaccgt cagcattggt gtggcgcatc acgaaggaaa tgaaagcatc
    16801 gatgaactgt ttaaacgcgt tgatgatgct ttgtatcggg cgaaaaatga tggacgcaac
    16861 cgcgtgctgg cggcataagc cgcggatgcg tctcgagatc aacgactgcg cttagcgtgg
    16921 cgctcccagt tttcttgctt cgcctgcgcc gttttacgta gtgcgacgta acacgccccg
    16981 ctgccgccat gatgcggtag cgcggtgcaa tatgcctgaa catcatcaaa ttcggtcagc
    17041 cagcgcgcca catagctgcg gacaatattg gcatgtgatt tatcttcccg ccctttacca
    17101 tgaataatca gcacattacg caaaccatcc gccagggctt gttgaatgaa actgaacacc
    17161 attttgcggc actcttccac cggctggcgc aaaagattca ggctcgcctg ttgcggatat
    17221 ttaccactgc gcagcttatc cagcacccca tgctgcaacc cttcctgccg aaactccagc
    17281 ggctgactta gcgggatgat gtcgagaaat ccggtagtga ggaaattatc aagctgcagc
    17341 gtgtcgatac gctgcggcgc acgttggtta cgcgttggat gccagtggac atctgtagca
    17401 cgtttcagcg gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc
    17461 atgtaacctc ctatgcacct tcacacgtcg tgattcagac gggttaattg ttcatactta
    17521 ctgcatcgtt cccttcacag attgaaacaa tactctactt catacctgtg agtatctgcg
    17581 caggcggggc attttatact agcctgttag cagtatcgaa cagtttagcc cgagtttaat
    17641 tcagcacagg ctccgatcag gatgatttat tcatagcagg aaccaacaaa aatgcgataa
    17701 ataacagacc actgattagc agaaaggtac cgaaaatgcc atagtttgcg cctccctcag
    17761 catcaattga ctcaataatt tggttgaagc cagtatttcc gatgtctggt cacttctact
    17821 gcgattaaaa aataaagcac ttctggaaat gaagtcattc accgtatggg gaacagaaac
    17881 cattttttca cccattctcg aaaaatgaaa attagttcga ttattgagaa tagagccaca
    17941 cagggcaata ccaatcgcgc ccccaagatt acgtgttaaa ttaaatacac cagaggctaa
    18001 cttcagccgt tcttttggaa ttccccccag cgttaatgtt acgatggggg ccatcgcaaa
    18061 ctgctgcaaa atccctctaa ttgcctgtgg aaacagtaat tcctgccatc cccattcgtg
    18121 ggttataggg gtaaaaagat acattgaaaa cacaaatccg cccagccccg ccatcaacag
    18181 ccatcgcaga tttatttttt tacttaacca gaagtaaaag ggaacagaaa ataactggaa
    18241 aatacctgtt gtgcaaacgg caaaacctat ctcttcggca tttaatccgc gaatctgccc
    18301 caaaaacaca ggtattaaat atacagtaga gaatatccca acccctccag aaaaagaaaa
    18361 aaaacatccc agagtgaaat ttttatcctt aaaagcatga agatccatta tcggattgga
    18421 tattgtaagt gtacgcgcag caaataaaat aaaactcacc aatgccagaa ctgatgtcag
    18481 caaaatagta ttatcatcca gccatcccca gcgcgctcct tcttcaaggg tatattccag
    18541 acatcccaga gtcatggcca acaggataat actcggataa tcagcaactt tcagtaaaga
    18601 gagatcaggt ttatcaaaat taaccagaaa aggaatactc aaaactaaat atatgccagg
    18661 taatatattg atataaaata gccatcgcca gtcgaggtta tcagtgagcc agccccccag
    18721 agttggccct agcgtcggtg acagtgaggc cagcgcactg actaccgcag cagccaaccc
    18781 caactcttta ccctggtaat aaataaacgc cgtcgtgaaa actaaaggga tcatggatgc
    18841 gccagcagct ccctgtaaag cacgaaacaa aatcatgatc tgaatattcc atgcaagtcc
    18901 acacgcgata ctcattaaag taaatattcc cgcggataga gtaaaaagcc aacgagtgga
    18961 aaatacccgt gacagccacc cagagagcgg aataacaata atctcagcaa ttaaatagct
    19021 ggtctgaagc caggccattt cgtctttacc tgcggacaga ccaccaccta tttcatttag
    19081 ggatgacgat acaatttgaa tatcaagctg agcaataaat aatcccatac acatactggc
    19141 gaaagcaata tgtttacgca tagacatcat ctctcatcaa cactgacggt gacggacagt
    19201 cccgggcgaa gaagttgctt taattccttt gcgtcgtcaa atgcaatccg taccgggaca
    19261 cgctgaacaa ttttggtgaa attgccagtg gcattttcta tcggaatcaa gctgaaactg
    19321 gctcctgtcg ctggggaaag gctttcaatg tgaccgtgaa acacttcccc tttgagaata
    19381 tcagcacgga tctcagcctt cattcctggc ttcattcccg ctatttggtt ttctttataa
    19441 tttgcatcaa cccacaactc actgactggt actagtgaaa ctaacgatgt gccgccttcc
    19501 acccaactgc ctgtatgggc gctgcggttt gcgacaatgc catcaatcgg cgca
//
LOCUS       DAJTRQ010000071        19389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000071 DAJTRQ010000000
VERSION     DAJTRQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(43..1872)
                     /gene="glmS"
                     /locus_tag="OO332_002535"
     CDS             complement(43..1872)
                     /gene="glmS"
                     /locus_tag="OO332_002535"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5942971.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="OO332_002536"
     CDS             complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="OO332_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5942972.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="OO332_002537"
     CDS             complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="OO332_002537"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5942973.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="OO332_002538"
     CDS             complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="OO332_002538"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5942974.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="OO332_002539"
     CDS             complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="OO332_002539"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5942975.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="OO332_002540"
     CDS             complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="OO332_002540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5942976.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="OO332_002541"
     CDS             complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="OO332_002541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5942977.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="OO332_002542"
     CDS             complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="OO332_002542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5942978.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="OO332_002543"
     CDS             complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="OO332_002543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5942979.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="OO332_002544"
     CDS             complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="OO332_002544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5942980.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="OO332_002545"
     CDS             complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="OO332_002545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5942981.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="OO332_002546"
     CDS             complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="OO332_002546"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5942982.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="OO332_002547"
     CDS             complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="OO332_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5942983.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="OO332_002548"
     CDS             complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="OO332_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5942984.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="OO332_002549"
     CDS             complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="OO332_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5942985.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15358..16350
                     /gene="asnA"
                     /locus_tag="OO332_002550"
     CDS             15358..16350
                     /gene="asnA"
                     /locus_tag="OO332_002550"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5942986.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="OO332_002551"
                     /pseudo
     CDS             complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="OO332_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="OO332_002552"
     CDS             complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="OO332_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5942987.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4631 a         5126 c         4864 g         4768 t
ORIGIN      
        1 gcattacacc ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga
       61 ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
      121 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
      181 ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata
      241 caattgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc
      301 gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg
      361 tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa
      421 tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt
      481 gtcagagaaa tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat
      541 acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc
      601 tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc
      661 tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc
      721 gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc
      781 tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat
      841 catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac
      901 gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata
      961 agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt
     1021 cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt
     1081 tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc
     1141 gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt
     1201 atcgaagatg tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat
     1261 aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat
     1321 acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg
     1381 ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc
     1441 ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt
     1501 gtcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg
     1561 gttttcgatg atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt
     1621 agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg
     1681 cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg
     1741 agtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc
     1801 cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac
     1861 aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt
     1921 tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg
     1981 gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt
     2041 ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat
     2101 gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct
     2161 actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg
     2221 gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg
     2281 ttatcgccaa tttccgcatc gcctaggtaa gtcagatgcc cggctttcgt gcctttaccc
     2341 agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca
     2401 ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc
     2461 acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa
     2521 ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta
     2581 tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg
     2641 cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc
     2701 agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga
     2761 acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat
     2821 tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatacc tgcgccgttg
     2881 gcaatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg
     2941 tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga
     3001 tcatccagtt tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc
     3061 tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca
     3121 tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga
     3181 agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg
     3241 tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc
     3301 atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct
     3361 ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta
     3421 atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa
     3481 tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg
     3541 accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg
     3601 tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg ggttttatat
     3661 cagcaggatc tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa agccagcctg
     3721 tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg
     3781 ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg
     3841 tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct
     3901 tcgtcgagat cctggccgcg aattgcagtg tcggccagaa cggtcacgtt gccaggctgc
     3961 acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg
     4021 cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt
     4081 tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct
     4141 gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa
     4201 gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg
     4261 gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg
     4321 agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga
     4381 agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca
     4441 tacccaggat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc
     4501 cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg
     4561 agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca
     4621 cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag
     4681 gtacgtatac cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct
     4741 gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc
     4801 ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca
     4861 gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa
     4921 cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga
     4981 taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta
     5041 cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca
     5101 tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga
     5161 acggacacat caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca
     5221 gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc
     5281 ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta
     5341 ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg
     5401 aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca
     5461 ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct
     5521 gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct
     5581 taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag
     5641 ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag
     5701 gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc
     5761 caggttttca accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc
     5821 cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc
     5881 atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt
     5941 aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa
     6001 catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac
     6061 ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat
     6121 tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt
     6181 gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc
     6241 cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc
     6301 aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc
     6361 ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt
     6421 gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc
     6481 aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg
     6541 aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg
     6601 ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg
     6661 aagctgccaa ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga
     6721 accagagact gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc
     6781 actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga
     6841 gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt
     6901 ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc
     6961 gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg
     7021 gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc
     7081 agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat
     7141 tcggcgttaa cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc
     7201 gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca
     7261 gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat
     7321 tcgcccattg cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg
     7381 gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg
     7441 ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg
     7501 cgctggttga tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg
     7561 atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc
     7621 tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca
     7681 gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc
     7741 acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact
     7801 ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc
     7861 tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata
     7921 caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg
     7981 ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt
     8041 tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc
     8101 tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct
     8161 gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc
     8221 atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct
     8281 acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg
     8341 agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc
     8401 agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca
     8461 gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc
     8521 tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg
     8581 cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt
     8641 tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc
     8701 cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg
     8761 cttcaatttc agcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt
     8821 tcgcttcgtc cagaatctgc gagcggagtt tattcgcctg ctcgatgatt acctgggctt
     8881 ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat
     8941 gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg
     9001 ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg
     9061 cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc
     9121 tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga
     9181 cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga
     9241 gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg
     9301 aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc
     9361 atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa
     9421 cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag
     9481 aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac
     9541 attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc
     9601 accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct
     9661 taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt
     9721 caactctttc gtgaagccgc cgatgccttt cattttgatg ttgtagaaca gaatcaggat
     9781 aaatacgccc agtgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag
     9841 tgcaggcaga cccagtacat gttcagcaat gtacggcagc aagtcgatag gcagtaaatc
     9901 catcagattc atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt
     9961 gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat
    10021 cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa
    10081 aaccaagaac aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca
    10141 gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag
    10201 gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc
    10261 ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc
    10321 cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac
    10381 accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaagc cacccggcct
    10441 ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga
    10501 aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac
    10561 agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt
    10621 cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc
    10681 gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata
    10741 cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat
    10801 ttttccagtg catacaattg cgacttttcc gctacccctg ttcgatcatg aaaaactgta
    10861 aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg
    10921 cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta
    10981 aaaacagata ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt
    11041 taataatgtt ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt
    11101 tcactaatga cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag
    11161 agtgaacgtg gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca
    11221 gtctaaccgg tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat
    11281 caccaccaga tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac
    11341 ctgatattct tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta
    11401 gaaacggcct tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc
    11461 aaaagcgcgg ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct
    11521 ctgtactggt tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac
    11581 gcgtttacca aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg
    11641 aatgcctggc agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg
    11701 tgccaccaca atgctatcga gaatatggcg taccagcatc tcattaggat cgcggaccga
    11761 agtcaggttg tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt
    11821 tttctggtga tcagtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag
    11881 cacggtgatt acctgttctt gatgcgttgc ttggtaagcg ggtgcttacc aggcattttt
    11941 aatgcgttat gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga
    12001 gatggccgca ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ccggtttgtg
    12061 atcgttaagt ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt
    12121 cgcgggtagc agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc
    12181 gatataacct tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa
    12241 cgcaggggca aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag
    12301 cagatcttca ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc
    12361 tgcagcttcc gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat
    12421 attctcaagt ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg
    12481 accgatttca gtcaaacgca gatccgcatt atcttcgcgc agcatcagac gatattctgc
    12541 gcgcgaagta aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaaccag
    12601 tacgccgaga tacgcctgag aacgtgccgg agcccaacct tctttgtcag cagacagacg
    12661 ggcagcgtta agaccggcca gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc
    12721 gttaatctga ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag
    12781 gtcgcgagga tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt
    12841 ttccatcccc tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga
    12901 gataccgttc ggataaattt cattagaggt cagtccttcc ggttcaagga agatctgatg
    12961 ctgatttctg tctgcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc
    13021 gacaccttcg atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc
    13081 atgggttttc tcgttggtat gagtgatata acacggcacc tgctggggat gcagggacgc
    13141 attgcccata aacgagaata ccggcattgg gttatcgcca tgttgttgcg ccagcacgct
    13201 aaagtcgatg gttcgagcat caatacgcgg cggagtaccg gttttcagac gaccaacgcg
    13261 cagcggcagt tcacgcaaac ggcgagagag cggaatggat ggcggatcgc cagcacggcc
    13321 accgctgtaa ttatccagac cgatatgaat tttgccgtcg aggaacgtcc caacggtgag
    13381 caccacagct ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc
    13441 gttttcgaca ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc
    13501 cagcgccgta cgtaccgcct gacggtagcg cacacgatcc gcctgagctc gggtagcgcg
    13561 aaccgccggt cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc
    13621 tttcgccatc agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc
    13681 gccgatcgcc gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag
    13741 agtctgttga cccatacgcg ccgcagccat cgcggcctcg gtgcctgcat gacccccgcc
    13801 aatgatgatg acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt
    13861 atgaaaatgg attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga
    13921 cctgggatcc tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat
    13981 ctcttattag gatcgcactg ccctgtggat aacaaggatc cgacttttaa gatcaacaac
    14041 ctggaaagga tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag
    14101 aatgaggggt tatacacaac tcaaaaactg aacaacggtt gttctttgga taactaccgg
    14161 ttgatccaag cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt
    14221 gagtaaatta atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc
    14281 aagaatgttg atcttcagtg tttcgcctgt ctgttttgca ccggaatttt tgagttctgc
    14341 ctcgagttta tcgatagccc cacaaaaggt gtcatattca cgactgccaa taccgattgc
    14401 gccaaagcgg accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga
    14461 aaggttgtcc ggaatatctc cggcaccgtg ggtggagctg ataaccagcc agatccccga
    14521 ggcagataaa tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc
    14581 cagcttttca gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat
    14641 aagagtgata tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga
    14701 acgcgttggg atctacctgt ggaaaagtat gggattaaaa aagccgatca gggcttgatg
    14761 gtacgcatga tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt
    14821 gtttggatct tgttgataag tacatgctgg agagcgtcga tcgaacggca cataactttt
    14881 ataaagatgc tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc aaggctttcc
    14941 agctttgcca gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag
    15001 cctacgtcat aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc
    15061 gcctgcttca ttttctctac tcgaacgtga atcgtccccg gactgacgcc aaattgtttc
    15121 gccagttcgg cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc
    15181 agattgtcga tcagataatt ttccatagga ttttcttatg cagattgatg attcattcta
    15241 ttttagcctt cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg
    15301 catgtgtgtc ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg
    15361 aaaaccgctt acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc
    15421 caactggaag aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg
    15481 gatggcacgc aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct
    15541 ctgcctgatg cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta
    15601 gggcaacacg acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc
    15661 gatgaagacc gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc
    15721 gtaatgggcg acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg
    15781 gcgggaatta aagcaaccga agctgcggtt aacgaagagt ttggcctggc accgttcctg
    15841 ccggatcaga ttcacttcgt acacagccag gagttactgt ctcgttatcc ggatctcgat
    15901 gccaaagggc gtgagcgggc gattgcgaaa gatcttggcg cggtattcct cgtcgggatt
    15961 ggcggcaagt tgagtgatgg tcatcgtcac gacgtgcgcg caccggatta tgatgactgg
    16021 agcaccccgt cagagctggg ccatgcgggc ctgaacggcg atattctggt gtggaacccg
    16081 gtactggaag atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgctg
    16141 aagcatcagc tggcgctgac cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg
    16201 ctgctgcgcg gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgact
    16261 atgctgctgc tgcaactgcc gcatatcggc caggttcagt gtggagtatg gccagttgct
    16321 gttcgcgaga gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc
    16381 ggcttcgcat cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt
    16441 tgccgtgtgc cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt
    16501 tcactttact cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg
    16561 catcggcatc aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg
    16621 caagatcggt gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt
    16681 gtgggcctga aagctcataa cggacgatct cggtggaaaa tagcataata taacagcgcc
    16741 ggttttcagc gagagcaatg cgcatcaaag ccaggcagaa cgctttcgca cactgttcat
    16801 taaagccgcc cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt
    16861 catcgtaatc tttatgtacc accggacgtt cgagcacttt ttcacgccac gactcaccgt
    16921 gcaggcgata ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt
    16981 ccgttatccc tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt
    17041 gcagaccatc aacctgctca ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt
    17101 ccatctacgc atcgttgcgc ggtattgatt tggcttcccg agaacgcccc ggctgctctg
    17161 ccaggcgttt cagttccggc tgttcgttaa gaaattcacc gtatttcaca ataaactgat
    17221 agtcgccacg tttaagctga ccggcgctca tatcccacag acgaccagct gcggtattgt
    17281 tatctgcgag aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactta
    17341 acagttgttc gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca
    17401 gacttaaacg ccaacgctgg agaaaaagcg tgtgtaacgc tgaagttatt gtgctgttag
    17461 catcaaccaa ctgccgggct tgttctgccc atggggaatt cagacgatgc agtaagtcta
    17521 ggatctgtgg tagctgcaca ataaactgcg gcgtggagag gagctggctt tgctgatagc
    17581 acatcacctc ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg
    17641 cctcacgcca gcggggaaca tcatcagtga ttgccgcctt cagtcgtggg aatttttcaa
    17701 agaagaccgc cagctgcggt gaggccagca gtgcgatgat catctcttcg atcaatccct
    17761 cttcgctgac ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg
    17821 aatctgtgca ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat
    17881 aaacagacat ttttgctgtt cgctgaataa agcgtgttgc ttacgccagt cgctttcaag
    17941 ctcttccagt tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc
    18001 cagcgtactg ccttgtaaac ttacatcgcg tacaacaaga tgttgggtgc tatcaacttc
    18061 cagattcagt ttctgggcaa agccgatacc gttcagtttg ccgcgaattt caccaccttt
    18121 actcagccac tgttccagcg cgctacgctc aaaggagata tgaaccactt ccatatcatg
    18181 cagttttaac ggtttttgca gcagcagagt cagagtggaa gcagtaacgt taacaggaag
    18241 ttgatactgc tgacgacggc tgaaaatgcc gcccagacga attaacgtta aggctgtttt
    18301 atcgctttgt tgctgctgga gttgcaggtg acgttgcaca atcgcgccca ggcgggtcaa
    18361 catcccttgc tgttgccagg catgaccggt catcaataca tcaatttgtt gttgtatcaa
    18421 attcaggctt tgcgcgtcat accacaggca atctttcagc aaaatgagat caaccggggc
    18481 aacagcactg cgaccgctaa aaaaggcgct ggcctgcaat aagcggatcg cttttttcca
    18541 gcgacgatcc gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa
    18601 aatcagctca aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc
    18661 atattcttca tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg
    18721 actggtcagc atggagcgga aattcgcttt atcttgcact ttatctaacc atagacgaat
    18781 cagcatgcgg tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc
    18841 cgccaccagt agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc
    18901 gttaatggcg gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag
    18961 aaagacgatt tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc
    19021 ttttagcgcc tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat
    19081 cagatattca aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact
    19141 tttggcaata cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa
    19201 acacaagcgg atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg
    19261 ggaaattctt tccgctaata aatgagggtg agccataatg aagtggcgtc ctttcgtcaa
    19321 aagttctgcg taaattgcga gtatagacgt ttcttgctgg tggctaaaat agtctcaaag
    19381 gggggtaat
//
LOCUS       DAJTRQ010000072        19244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000072 DAJTRQ010000000
VERSION     DAJTRQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1868
                     /gene="scpA"
                     /locus_tag="OO332_002553"
     CDS             <1..1868
                     /gene="scpA"
                     /locus_tag="OO332_002553"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5942988.1"
                     /translation="RTLSSKQGLSVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVED
                     MKVLFDQIPLGKMSVSMTMNGAVLPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLC
                     RNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISGYHMGEVGANCVQQVAFTLADGI
                     EYIKAAISAGLKMDDFAPRLSLFFGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKS
                     LALRTHCQTSGWSLTEQDPYNNVIRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSA
                     RIARNTQIIIQEESELCHTVDPLAGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIE
                     AGLPKRMTEEASAREQSLIDQGKRVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASL
                     ERIRAARDDAAVTAALNALTHAAQHNENLLAAAVNAARVRATLGEISDALEVAFDRYL
                     VPSQCVTGVIAQSYHQSEKSASEFDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGA
                     KVIASAYSDLGFDVDLSPMFSTPEEIARLAVENDVHVAGASSLAAGHKTLIPELVEAL
                     KKWGREDICVVAGGVIPPQDYAFLQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            1861..2856
                     /gene="meaB"
                     /locus_tag="OO332_002554"
     CDS             1861..2856
                     /gene="meaB"
                     /locus_tag="OO332_002554"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5942989.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            2867..3652
                     /gene="scpB"
                     /locus_tag="OO332_002555"
     CDS             2867..3652
                     /gene="scpB"
                     /locus_tag="OO332_002555"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5942990.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            3676..5154
                     /gene="scpC"
                     /locus_tag="OO332_002556"
     CDS             3676..5154
                     /gene="scpC"
                     /locus_tag="OO332_002556"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR5942991.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5096..6046)
                     /locus_tag="OO332_002557"
                     /pseudo
     CDS             complement(5096..6046)
                     /locus_tag="OO332_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6213..6953)
                     /locus_tag="OO332_002558"
     CDS             complement(6213..6953)
                     /locus_tag="OO332_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5942992.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(7046..7681)
                     /gene="argO"
                     /locus_tag="OO332_002559"
     CDS             complement(7046..7681)
                     /gene="argO"
                     /locus_tag="OO332_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5942993.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(7820..8680)
                     /gene="mscS"
                     /locus_tag="OO332_002560"
     CDS             complement(7820..8680)
                     /gene="mscS"
                     /locus_tag="OO332_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5942994.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(8871..9950)
                     /gene="fbaA"
                     /locus_tag="OO332_002561"
     CDS             complement(8871..9950)
                     /gene="fbaA"
                     /locus_tag="OO332_002561"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5942995.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(10165..11328)
                     /gene="pgk"
                     /locus_tag="OO332_002562"
     CDS             complement(10165..11328)
                     /gene="pgk"
                     /locus_tag="OO332_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5942996.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(11378..12397)
                     /gene="epd"
                     /locus_tag="OO332_002563"
     CDS             complement(11378..12397)
                     /gene="epd"
                     /locus_tag="OO332_002563"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5942997.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            12769..13200
                     /locus_tag="OO332_002564"
     CDS             12769..13200
                     /locus_tag="OO332_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5942998.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            13223..13801
                     /locus_tag="OO332_002565"
     CDS             13223..13801
                     /locus_tag="OO332_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5942999.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            13802..14110
                     /locus_tag="OO332_002566"
     CDS             13802..14110
                     /locus_tag="OO332_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943000.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            14126..14509
                     /locus_tag="OO332_002567"
     CDS             14126..14509
                     /locus_tag="OO332_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5943001.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNELAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            14497..15174
                     /locus_tag="OO332_002568"
     CDS             14497..15174
                     /locus_tag="OO332_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5943002.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            15168..15845
                     /locus_tag="OO332_002569"
     CDS             15168..15845
                     /locus_tag="OO332_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5943003.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(15817..16530)
                     /locus_tag="OO332_002570"
     CDS             complement(15817..16530)
                     /locus_tag="OO332_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5943004.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(16527..17036)
                     /gene="fumE"
                     /locus_tag="OO332_002571"
     CDS             complement(16527..17036)
                     /gene="fumE"
                     /locus_tag="OO332_002571"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5943005.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(17058..18023)
                     /gene="yggF"
                     /locus_tag="OO332_002572"
     CDS             complement(17058..18023)
                     /gene="yggF"
                     /locus_tag="OO332_002572"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5943006.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(18020..>19244)
                     /locus_tag="OO332_002573"
     CDS             complement(18020..>19244)
                     /locus_tag="OO332_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5943007.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
BASE COUNT         4631 a         5171 c         4810 g         4632 t
ORIGIN      
        1 tccggacatt atcttccaaa caaggtcttt ccgttgcgtt tgaccttgcc acccaccgtg
       61 gctacgactc cgataaccca cgcgtggcgg gtgacgtcgg caaagcgggc gtcgctatcg
      121 acaccgtgga agatatgaaa gtcctgttcg accagatccc gctgggtaaa atgtcggttt
      181 cgatgaccat gaatggcgca gtgctaccag tactggcgtt ttatatcgtc gccgcagaag
      241 agcaaggtgt tacacctgat aaactgaccg gcaccattca aaacgatatt ctcaaagagt
      301 acctctgccg caacacctat atttacccac caaaaccgtc aatgcgcatt atcgccgaca
      361 tcatcgcctg gtgttccggc aacatgccgc gatttaatac catcagtatc agcggttacc
      421 acatggggga agtgggtgcc aactgcgtgc agcaggtagc atttacgctc gccgatggga
      481 ttgagtacat caaagcagca atctctgccg gactgaaaat ggatgacttc gctcctcgcc
      541 tgtcgttatt cttcggtatc ggcatggatc tgtttatgaa cgtcgccatg ttgcgtgcgg
      601 cacgttattt atggagcgaa gcggtcagtg gatttggcgc acaggatccg aaatcactgg
      661 cgctgcgtac ccactgccag acctcaggct ggagcctgac tgaacaggat ccgtataaca
      721 acgttatccg caccaccatt gaagcactgg ctgcgacgct gggcggtact cagtcactgc
      781 ataccaacgc ctttgacgaa gcgcttggtt tgcctaccga tttctcagca cgcattgccc
      841 gcaacaccca gatcatcatc caggaagaat cagaactctg ccacaccgtc gatccactgg
      901 ccggatccta ttacattgag tcgctgaccg atcaaatcgt caaacaagcc agagctatta
      961 tccaacagat cgacgaagcc ggtggcatgg cgaaagcgat cgaagcaggt ctgccaaaac
     1021 gaatgaccga agaggcctca gcgcgcgaac agtcgctgat cgaccagggc aagcgtgtca
     1081 tcgttggtgt caacaagtac aaactggatc acgaagacga aaccgatgta cttgagatcg
     1141 acaacgtgat ggtgcgtaac gagcaaattg cttcgctgga acgcattcgc gccgcccgtg
     1201 atgatgccgc cgtaaccgcc gcgttgaacg ccctgactca cgccgcacag cataacgaaa
     1261 acctgctggc tgccgctgtt aatgccgctc gcgttcgcgc caccctgggt gaaatttccg
     1321 atgcgctgga agtcgctttc gaccgttatc tggtgccaag ccagtgtgtt accggcgtga
     1381 ttgcgcaaag ctatcatcag tctgagaaat cggcctccga gttcgatgcc attgttgcgc
     1441 aaacggagca gttccttgcc gacaatggtc gtcgcccgcg cattctgatc gctaagatgg
     1501 gccaggatgg acacgatcgc ggcgcgaaag tgatcgccag cgcctattcc gatctcggtt
     1561 tcgacgtaga tttaagcccg atgttctcta cacctgaaga gatcgcccgc ctggccgtag
     1621 aaaacgacgt tcacgtagcg ggcgcatcct cactggctgc cggtcataaa acgctgatcc
     1681 cggaactggt cgaagcgctg aaaaaatggg gacgcgaaga tatctgcgtg gtcgcgggtg
     1741 gcgtcattcc gccgcaggat tacgccttcc tgcaagagcg cggcgtggcg gcgatttatg
     1801 gtccaggtac acctatgctc gacagtgtgc gcgacgtact gaatctgata agccagcatc
     1861 atgattaatg aagccacgct ggcagaaagt attcgccgct tacgtcaggg tgagcgtgcc
     1921 acgctcgccc aagccatgac gctggtggaa agccgtcacc cacgtcatca ggcgctaagt
     1981 acacagctgc ttgacgctat tatgccgttc tgtggtaacg ccctgcgact gggcgttacc
     2041 ggcacacccg gcgcggggaa aagtaccttt cttgaagcct ttggcatgtt gttgattcgt
     2101 gagggattaa aggttgctgt tattgcggtc gatcccagca gcccggtcac tggcggtagc
     2161 attctcgggg ataaaacccg catgaatgac ctggcgcgtg ccgaggcggc gtttattcgc
     2221 ccggtaccct cctccggtca tctgggcggt gccagtcagc gagcacggga attaatgctg
     2281 ttatgcgaag cggcgggtta tgacgtagtg attgtcgaaa cggttggcgt cgcgcagtcg
     2341 gaaacggaag tcgcccgcat ggtggactgt tttatctcgt tgcaaattgc cggtggcggc
     2401 gatgatctgc agggcattaa aaaagggctg atggaagtgg ctgatctgct cgttatcaat
     2461 aaagacgatg gcgataacca taccaatgtc gccattgccc ggcatatgta cgagagtgcc
     2521 ctgcatattc tgcgacgtaa atacgacgaa tggcagccac ggggtctgac ttgtagcgca
     2581 ctggaaaaac gtggaatcga tgagatctgg cacgccatca tcgacttcaa aaccgcgcta
     2641 actgccagtg gtcgtttaca acaagtgcgg caacaacaat cggtggaatg gctgcgtaag
     2701 cagaccgaag aagaagtact gaatcacctg ttcgcgaatg aagatttcga tcgctattac
     2761 cgccagacgc ttttagcggt caaaaacaat acgctctcac cgcgcaccgg cctgcggcag
     2821 ctcagtgaat ttatccagac gcaatatttt gattaaagga atttttatgt cttatcagta
     2881 tgtgaacgtt gccactatca acaaagtggc ggtcattgag tttaactatg gacgaaaact
     2941 taatgcctta agtaaagtct ttattgatga tcttatgcag gcgttaagcg atctcaaccg
     3001 gccggaaatt cgctgtatca ttttgcgggc gccaagtgga tccaaagtct tctccgcagg
     3061 tcacgatatt cacgaactgc cgtctggcgg tcgcgatccg ctctcctatg atgatccatt
     3121 gcgtcaaatc acccgcatga tccaaaaatt cccgaaaccg atcatttcga tggtggaagg
     3181 tagtgtttgg ggtggcgcat ttgaaatgat catgagttcc gatctgatca tcgccgccag
     3241 tacctcaacc ttctcaatga cgcctgtaaa cctcggcgtc ccgtataacc tggtcggcat
     3301 tcacaacctg acccgcgacg cgggcttcca cattgtcaaa gagctgattt ttaccgcttc
     3361 gccaatcacc gcccagcgcg cgctggctgt cggcatcctc aaccatgttg tggaagtgga
     3421 agaactggaa gatttcacct tacaaatggc gcaccacatc tctgagaaag cgccgttagc
     3481 cattgccgtt atcaaagaag agctgcgtgt actgggcgaa gcacacacca tgaactccga
     3541 tgaatttgaa cgtattcagg ggatgcgccg cgcggtttat gacagcgaag attaccagga
     3601 agggatgaac gctttcctcg aaaaacgtaa acctaatttc gttggtcatt aatccctgcg
     3661 aacgaaggag taaaaatgga aactcagtgg acaaggatga ccgccgatga agcggcagaa
     3721 attatccagc ataacgacat ggtggcattt agcggcttta ccccagcggg ttcgccgaaa
     3781 gccctacccg ccgcgattgc ccgcagagct aacgaacagc atgaggccaa aaagccgtat
     3841 caaattcgcc tgctgacggg ggcgtcaatc agcgccgccg ctgacgatgt actttctgac
     3901 gccgatgctg tttcctggcg tgcgccatat caaacatcgt ccagtttacg taaaaagatc
     3961 aatcagggcg cggtgagttt cgttgaccta catttgagcg aagtggcgca aatggtcaat
     4021 tacggtttct tcggcgacat tgatgttgcc gtcattgaag catcggcact ggcaccggat
     4081 ggtcgagtct ggttaaccag cgggatcggt aatgcgccga cctggctgct gcgagcgaag
     4141 aaagtgatca ttgaacttaa tcactatcac gatccgcgcg ttgcagaact ggcagatatt
     4201 gtgattcctg gcgcgccacc gcggcgcaat agcgtgtcga tcttccatgc aatggatcgc
     4261 gtcggtaccc gctatgtgca aatcgatccg aaaaagattg tcgccgtcgt ggaaaccaac
     4321 ttgcccgacg ccggtaatat gctggataag caaaatccca tgtgccagca gattgccgat
     4381 aacgtggtca cgttcttatt gcaggaaatg gcgcatgggc gtattccgcc ggaatttctg
     4441 ccgctgcaaa gtggcgtggg caatatcaat aatgcggtaa tggcgcgtct gggggaaaac
     4501 ccggaaattc ctccgttcat gatgtattcg gaagtgctac aggaatcggt ggtgcattta
     4561 ctggaaaccg gcaaaatcag cggggccagc gcctccagcc tgacaatctc ggccgattcc
     4621 ctgcgcaaga tttacgacaa tatggattac tttgccagcc gcattgtgtt gcgtccgcag
     4681 gagatttcca ataacccgga aatcatccgt cgtctgggcg tcatcgctct gaacgtcggc
     4741 ctggagtttg atatttacgg gcatgcaaat tcaacgcacg tagccggggt cgatctgatg
     4801 aacggcatcg gcggcagcgg tgattttgaa cgcaacgcgt atctgtcgat ctttatggct
     4861 ccgtcgattg ctaaagaagg caagatctca accgtcgtgc caatgtgcag ccatgttgat
     4921 cacagcgaac acagcgtcaa agtgatcatc accgaacaag ggatcgccga tctgcgcggt
     4981 ctttccccgc ttcaacgcgc ccgcactatc attgataatt gtgcacatcc tatgtatcgg
     5041 gattatctgc atcgctatct ggaaaatgcg cccggcggac atattcacca cgatcttagc
     5101 cacgtcttcg acttacaccg taatttaatt gcaaccggct cgatgctggg ttaatggttt
     5161 gtcccgtcac gatcttcagc aatatgctga agatcgtggc ggtataacga tgatttctga
     5221 ttgaataaag atattcctgc atatacatcg gcctgtgcgg cgcatcaaaa tatttcagcg
     5281 ttgccggatt caccagtcac caggcaaaat gcgggatcac cgtcagaaaa tgtccgcgct
     5341 ccacggcgct gattttggcc ataaaactgt agggacgata aataatggtg ggattgatcc
     5401 cacaaggtcg catattcgca tcaatcattg cttcgaagtt agcccggttt tgaaagcgca
     5461 tttgtagcca ggggagctcc tgaagcagct cttgctcttg acgctcttta taacggcgag
     5521 aaacgagaaa gcccaatcac agcggtggta attcactgat agtcaggttt tccagttcct
     5581 gaactcgtgc agatacatgc tgtggggaga taataaaatc taactgacgg tcaaaaagat
     5641 tatcgataac gccgttttcg ctgaactcaa ctggctgggc ggtcacgcct tcatacttat
     5701 ctccgagact aatcaactga tcgaaaataa ttgtcggata agtattatct atgccaatca
     5761 caatttctcg tgaacgacgc cctgagttat ggatttcatt atcaatcgca gaaagtcgtt
     5821 gataaaccgg gaacagtttt tgatacagtt cctgaccggc tttattgagg ctgatattat
     5881 tatctttgcg ggtaaatagc gtatagccaa tttgttcttc cagcgcggca atgcttttac
     5941 caaatggcga agccgtcata tggatttttt ctgccgcccg ggcaatatta ttggtttgcg
     6001 ctaataaaat gaaattgcgc atctttttcg aaataaaaat gtccatcccc cctccccgca
     6061 tccttgtccg cttaaccgtt tcagtcagcc tatccttgat gaaaccgcga gcaaagatag
     6121 gtgattacgt catggtttta cagaaaatta cagaaaaagg aggcaatatc gggtaaaggc
     6181 attagcccga cgaatacgtc gggctacaag tattattgtg ctgcaggtgt tttagcgggt
     6241 tgttgatcca caggttctaa ctggaagacc acatcgacct gatcatcaaa ctgaatagcg
     6301 gcctgctcgt aagtttcctg ggcagacacc ggcgcggcat cggctttcat catccgcacc
     6361 attgggctgg gctgatagtt ggaaacatgg tagcgcacgc tatataccgg ccccagttta
     6421 cgatgaaagc cgttcgccag ttcctgcgcc tgatgaatcg cgttatcaat cgctgcctta
     6481 cgcgctttgt ctttataggc atccggctgc gccacgccca gcgacacaga acgaatttcg
     6541 ttaagacccg ccttcagcgc gccatccagc aaggaattca gtttgtctaa ctgacggagc
     6601 gtgacttcca ccgttctcac agctcggtag cctttcagga tacttttacc atcctgatag
     6661 tcataatctg gctgggtgcg taaattcgct gagctgatat cttttttcgc gatctgattg
     6721 agttcaagga aggaaatgta ttgtgcgacg cgctcatctg cctgtttctt ggctgtagcg
     6781 gcatccttcg cggccacgtt aacttcaatc gcaagagtgg caatgtctgg caccgcatcc
     6841 acacttgccg taccggaggt gacaatatgc ggtccatccg gcaattcgtt agcctgcgct
     6901 gccatcccgc taatacccat taatgccgcc agggcgataa ctttgaactt cacgttagtc
     6961 tctccgtgtt aattgacttg cccaaaattc aggacctctg gaggcaagct tagcgcctct
     7021 gttttatttt tccatcagat agcgcctaac tgaacaaggc ttgtgcatga gcaataccgt
     7081 ctctcgccag ctgcaaggct ataaaccaca tgacacatcc cactaccaga ttgataatgt
     7141 gctgtgattt tgccgtgcgc aggcgcggtg ccagccaggc tgcgagaata gccagaccaa
     7201 agaaccacag gaaagaggcg ctaattgtcc cgagcgcaaa ccaacgtttt ggttcgacgt
     7261 caagctgccc gccaaggctg cccagtacaa caaaagtatc caggtaaaca tgcggattca
     7321 gccaggtcac tgccaacatg gtggcgataa ttttccatct gccttgcttc aggacttcgg
     7381 cactagctaa ctcaatatta ctactcatcg ctgttttaaa agcgccaaaa ccataccaga
     7441 gcaagaagac tacgccgccc caagtgacca gcgctagcaa ccacggcgac tgcatcaata
     7501 acgcgctacc accaaaaatc cccgcgcaaa tcaggaccaa atcgctgata gcgcaaagta
     7561 aggcaatcat aatgtggtac tgacgacgaa tgccctgatt catcacaaaa gcattttgtg
     7621 ggccgagcgg cagaatcata gctgccccaa gagcaagacc ttgaaagtaa taagaaaaca
     7681 cgttggatat tccgaattaa gtatctgatg cagagactat atcgcgggat tgttattagc
     7741 ggaaattgat aatattaatt ggcaatcaga ataactaata agagagaggc cagagagtat
     7801 tatctggcct cagcgttgat tacgcagctt tgtcttcttt cacccgctta aagttcacat
     7861 ccatttgcgg gtacgggaag ctgataccgg cggcatcaaa ttcacgttta atacgctcca
     7921 gcacatccca gtacacgttt tgcagatcgc cgctgttgct ccagacgcgg accacgaaat
     7981 taatcgacga tgcaccaagt tcgttcaggc gcacagtcat ttcgcgatct ttcaaaatgc
     8041 gatcttcaga ctggataata ttggtcagga tctgcttaac ctgatcgata tcggaatcat
     8101 acgccacgcc aataataaat tcgttacggc gaactggctc gcgggagaag ttaataatat
     8161 ttccggcaat aattttaccg ttcggaataa cgataatttt accgtctgca gtacgcatgg
     8221 tggtggagaa aatctgcaca ctcagcacag taccggctac gccgccgagg tcaacatatt
     8281 ctccggcacg gaacgggcgg aacatgacaa gtaacacgcc agcggccagg ttagaaagtg
     8341 acccctgcaa agccagacca acagctaagc ctgcggcacc gagtacagca atgactgacg
     8401 ctgtttgtac acccacgcgt cccagggcag cgattagcgt aaaggcgata ataccgtaac
     8461 ggactaatgc agaaagaaaa tcagcaacag tggcatcgat tttacgggag atcatcaggc
     8521 gattcaccgc gttggaaatc atccgcgcga taatcaaacc aacgatgatg atcgcgagtg
     8581 ccgccacgat gtttactgca taacttagca gcagcgcctg gttagctacc agccagcttc
     8641 ccgcgccgtt tatgctatcg acaacattca aatcttccat tcaatattcc ttttcaactg
     8701 actcaaaatg gagaaataca ctccgccgcc ttatgacggg cagtctgaca agggtaaaca
     8761 ataaagcata attttgccaa atagatcaca gatttctcat ctgaatcacc ttgcttttgg
     8821 cgaaaaaaag acccgcagag cgggccttga gataagcaga aaggaatgac ttacagaacg
     8881 tcgatcgcgt tcagttcctg gaatgctttt tccagacgag cgatcatcga agtctgaccg
     8941 gcacgcagcc atacgcgcgg atcgtagtat ttcttgttcg gctgatcttc gcctttcggg
     9001 ttacccagct gaccctgcag ataagcttcg ttcgctttgt agtagttcag aacgccttcc
     9061 caggttgccc attgggtatc ggtatcgatg ttcattttca ctacgccgta gcttacggag
     9121 tctttgattt cctgagcagt agaaccggaa ccaccgtgga atacgaagtt caggctgttg
     9181 tgcggcaggt tgtgtttctt agaaacatat tcctgagaat cacgcaggat ggtcggagtc
     9241 agaaccacgt tacccggctt gtaaacaccg tgtacgttac cgaaggacgc agcgatggtg
     9301 aaacgcgggc tgattttgct cagctcggtg tatgcgtaat caacgtcttc cggctgggtg
     9361 tacagtgcag aagcgtccat gtggctgttg tccacgccgt cttcttcacc accggtgcaa
     9421 cccagttcga tttccagagt catgccgatt ttggacatac gctccaggta tttagagcag
     9481 atttcgatgt tctcttgcag agattcttca gacaggtcga tcatgtgaga agagaacagc
     9541 ggcttaccgg tagctgcgaa gtgtttttca cccgcgtcca gcagaccgtc gatccacggc
     9601 agcagtttct tcgcgcagtg gtcagtgtgc aggataaccg gaacaccata atgttcagcc
     9661 atctggtgaa cgtgatgcgc accagagatc gcgcccagga tagcagcacc ctgcggaacg
     9721 tcagatttca cgcctttacc agcgataaag gaagcaccac cgttggagaa ctgaacgata
     9781 accggcgctt taactttagc agcagtttcc agtacggcgt tgatggagtc agtaccgacg
     9841 cagtttactg ctggcagtgc gaagttgttt tcttttgcta cctggaaaac tttctgtacg
     9901 tcatcaccag tgattacgcc aggttttacg aaatcaaaaa tcttagacat gtctcttgtc
     9961 ctgtatcgtc gggccttata ctcataatac ttcaagttgc atgtgctgcg tctgcgttcg
    10021 ctcaccccag tcacttactt atgtaagctc ctggggattc actctcttgt cgccttcctg
    10081 caactcgaat tatttagagt atgaaaaaat gcgtgctcta aaagcgcgct gaaacaaggg
    10141 caggtttccc tgccctgtga ttttttactt cttagcgcgc tcttcgagca tcgctactgc
    10201 aggcagtact ttaccttcca cgaattcgag gaatgcgccg ccgccagtgg agatgtagga
    10261 gattttgtca gcaatgccga acaggtcgat tgctgccaga gtgtcgccgc cgccagcgat
    10321 ggagaacgct tcgctgtctg cgatagcgtt agccacgatt tcagtacctt tgcggaagtt
    10381 cgggaattcg aacacgccaa ccggaccgtt ccacagaatg gttttcgcat tcttcaggat
    10441 ttcagccagt tcctgagcgg aagcatcacc gatatccagg atctgctcgt cagctttcac
    10501 atcgttaaca gatttcaggg tagccggtgc agtttcagag aactcggttg ctacgcgaac
    10561 atcagacgga accgggatgt tgcaggtggt cagcagacgt ttagcttcgt caaccaggtc
    10621 agcttcgtac agggatttac ccacatcgtg gccttgtgcc gcgataaagg tgttagcgat
    10681 accaccacca acaatcagct ggtcagcgat tttagacagg gagtccagaa cggtcagttt
    10741 ggtagatact ttagaaccac caacgatagc caccatcggg cgagcaggtt ctttcagtgc
    10801 tttacccagc gcgtccagtt cagctgccag cagcgggcct gcgcacgcaa cgtcagcgaa
    10861 tttaccgata ccgtgagtag aagcctgcgc gcggtgagca gtaccgaatg cgtccattac
    10921 gaacacgtca cacagtgcag cgtatttttt ggacagggtt tcgtcgtctt tcttctcgcc
    10981 tttgttgaag cgaacgtttt ccagaacaac cagttcacct tcagcaacgt caacgccgtc
    11041 gaggtaatct ttaaccagac gaaccgggtt agacagtttg tctttcaggt agttaacaac
    11101 cggcagcaga gagaattctt cgttgtactc gccttcggta ggacgaccca ggtgggaagt
    11161 taccatcact tttgcgcctt gtttcagggc cagttcgatg gtcggcagag aagcacggat
    11221 acgcgcgtcg ctggttactt tcccgtcttt tactggtacg ttcagatccg cacggataaa
    11281 tacacgtttc ccagcaagat ccagatcggt catcttaatt acagacatgg tgaatcctct
    11341 cgttgattct aaaagttttg cagacgctgc ttgcgtctta cctgaaagca acagtagcca
    11401 tagctaacgt cgtgtcgagc attcggttag caaagcccca ttcgttatcg caccagacca
    11461 acgttttgat caggtgtgcg ccactgaccc gggtttgggt gccatcgaca atggcactgt
    11521 gcggatcgtg gttaaaatct acagagacca acggcaattc cgtatagtca actataccat
    11581 gaaatgcacc ttgtgctgct ttttgcagca acaggttgac ttcattggct tttacaggtt
    11641 tcttcaccgt cacgcttaaa tcgattgccg tcacatttat ggttggcaca cgtaccgcaa
    11701 tcgcttcaaa gcgatcgtta aattgcggaa aaaatcgtgt gataccagcg gccagtttag
    11761 tatcgaccgg aatgatcgac tggctggctg cccgggtgcg acgcaggtca ggatggtatg
    11821 catcaataac ctgttgatcg tgcatggcgg agtgaattgt ggtcacagtg ccggactcaa
    11881 taccgtacgc atcatctaac aatttgatga cgggaattat gcaattcgtg gtacaggaag
    11941 cgttagaaac gatgcggtgt tccgcacgaa gttgatcctg attgacgccg taaacaacgg
    12001 tcgcgccgag atcgttactg ccaggatgtg aaaagagcac ttttttggcc ccggcggcaa
    12061 tatgcgcttc gctatgctcg cgggagccat atacgccggt gcagtcgagg actacatcaa
    12121 cgccaagttc acgccagggg agcgattgca gtgaacgttc atgcaatacg cggatggcgt
    12181 catcaccaac aaaaagttga tcgcgttcct gtcgtacttc ccatgcaaaa cggccatggc
    12241 tggtgtcata tttcaacaaa tgcgccatgc ccgcagcatc cgccagttcg ttgattgcca
    12301 ccacggtaat ttccgcccgg cgtccggatt catacaaagc acgaaccaca ttacgcccga
    12361 tgcgaccgaa gccatttatc gctacgcgta cggtcataga tctcctgcaa ggttttccct
    12421 gagcaaattt gccagacaga gtaatctagc aaatcgtccg gggaaacctt acctgtcgca
    12481 aactgcgact gattggttaa ttgtcgaaca tttaatcgac tgaaacgctt cagctaggat
    12541 aagcgaaacg tggaataaaa ggaatgattg tccagtcaaa gaaagcaatt atctgactta
    12601 cgtcacgttt tcagccccgc ccctgtggaa tttattccac tttgcagaat cacgaataag
    12661 cgtagcaaaa tcttttcgtt gtgccgtcac aaaccctgac ttatatcaga atatggctga
    12721 tgcagcgtcg atattatccg caatacgtcc gagtttcgga gtactccaat gatgacattg
    12781 ccctacccat attcttcctc cgcacgcttt tacgcactgc gcctcctccc cgggcaggaa
    12841 gttttttccc agctacacgc ttttgcacag caacatcaac ttcatgccgc ctggattgca
    12901 ggctgtaccg gtagcctgac tgacgtcgcc ttgcgctatg ccgggcaaga aggcaccacg
    12961 ctcctgaacg gtacattcga agtgatctca ctaaacggca cgctggaaca gagtggtgag
    13021 catcttcatc tctgcgtttc cgatccacac ggcacgatgc ttggcggtca tatgatgccc
    13081 ggctgtaccg tgcggaccac gcttgaactg gtgatcggta gccttgagga actggagttt
    13141 agccgccaac cttgcgcgct ttctggttac gacgagttgc atatttcccc cgttaaataa
    13201 ctactttcaa agaggttttc ttatggcgcg tagccacttt tccagtcagg cactggtgtt
    13261 gatcgttata tctatcgcca tcaatatgat cggcgggcaa ctggccagca tggtcaaatt
    13321 acctatattt ctcgactcca tcggcacact tattagcgcg gtactgcttg gtccggtgat
    13381 cggaatgctg accggcttac tgaccaattt actctgggga ttgctgaccg acccgatcgc
    13441 cgccgcattt gcacccgtcg cgatggtcat cggcctggtc tccggttggc tggcgcgagc
    13501 gggttggttt cgcaccctgc caaaagtcgt tgtcagcggc gtgattatta cgcttgcggt
    13561 aacggtcgtc gctgtaccgc tgcgtactgc gctgttcggc ggtgtcaccg gtagcggagc
    13621 cgatttgttc gttgcctgga tgcactcgat ggggcaaaac ctggtggagt cggtggcgat
    13681 tacggtgata ggcgcgaacc tggtcgataa aattcttaca gcggtgattg tctggctttt
    13741 actgcgccag ctgccgattc gcaccacgcg ccattttccg gcaatggctg ccgtgcgcta
    13801 aatgcatcca tttacctcat taacactgtg ggcattagcg gcctgcacca cgcttatcct
    13861 gcccgcgcag accattttgc cgatctatag cgcggcgacc tttttctgcc ttattgcgct
    13921 aaaggccacg cggcggcggg caaaatatgt cgtctggctg atgttctcgc tgggagccgg
    13981 gctgtggctg gtacacggtg gctggctacc agaatggcta agcggcacgc cacgctcgcc
    14041 agaacggtgg tcgcatgcta ttacgctctg gttgctgatt ctggcgattg tttcaacctc
    14101 acagttgtga atgcagtacg tccccgtgca atgttttatt cgcgctctgt ttgcttctcg
    14161 cctgccgcca ggcgtcgctt atctgtttgc cgggccgtta ttggtcgttg agcagttaaa
    14221 acggcaatta gcgattattc atgaagcgca gcgtgcccgc ggcgtgccgc tggatgaggg
    14281 ctggtatcag cgtttacgtg ctatgcccgc gttaattatt ccgctaacac acaatgcgct
    14341 taacgagctg gctgtccgtg gtgcggcgct ggatatgcgg gcatttcgga tccacaaccg
    14401 acgaacaacg ttgtgggcac cagccgatag caccctacaa cgcgtagcgc gttacaccat
    14461 gatcttgctg atgctgacag aattcggagc gtggatatgg ttacgttaga acagttccgt
    14521 tattgcccga cacactcaac gcatcctccg ttctgttatg actttcacta tgtcaaaccg
    14581 gggatggtcg ccatctttgg tgacaacggc agtggcaaaa gtacgctggc acagctgatg
    14641 gcgggctggt atccggactt tctgccaaga gagataacag gcacggggac gctcctcggt
    14701 acgcccattg ggcatctccc gcttaatgaa caatccgcca ccatccagct cgtccagcaa
    14761 tcgccctatt tgcagctctc cggctgtacc tgtagcgtgg aagaagaagt tgcctttggg
    14821 ccagaaaatc tgtgtcttgc tgaagcagag attatggcgc ggattgatgc cgccctgact
    14881 ctgaccgagt gccagccgtt gcgccatcgt cacccggcaa cactctccgg tggagagaca
    14941 cagcgcgtgg tcatcgcctg cgccatcgcg atgcagccaa agctcttaat tcttgatgaa
    15001 gcctttagcc gcctgacgcc gcaggccagc gaaatgctgt tgcagcgctt acaacactgg
    15061 gcgtttgaac gtggttcgct gattattctt tttgaacgtc atcgcacacc tttcctgaac
    15121 tactgccagc aagcgtggca attgcaaaac ggagcgctcc agccactatg ttaacgttaa
    15181 ataagatatc ttatcgctgg cctggcgcgg caacggattg tctttgcgac atttctctgc
    15241 aactcaaaca aggtgaatgg ttggcgctga ccggtgacaa tggcgcaggg aaatcgacgc
    15301 tgttacgagt aatggcaggt cttcttacgc ccacagccgg tacggttatg ctgcaacaac
    15361 aggcgatgaa aaacctgaaa aaccgccagc gcgcggcaaa ggttggcgta ctgtttcagg
    15421 aagcggaaaa ccaacttttt cacagcaccg tcgctgacga gatcgcattt ggtttgaaac
    15481 tgcaaaaatg cccggcggat gaaatcactc aacgtaccca tgctgccctg caatgttgtc
    15541 agttagcaga cactgccaac tcgcacccgt tggatctgca tagtgcgcag cgacggatgg
    15601 tcgccgtcgc ctgcctggaa gcactctctc cgccactatt actgctggac gagccgagtc
    15661 gggattttga cgaaaattgg ttgagcgtgt ttgaaagctg gctggagaag tgccgccagc
    15721 gaggcacgag cgtggtagca atcagtcatg acgccgcatt tacccggcgt catttctctc
    15781 gtgtggtgcg actggaagat ggtgtaatca ggaacgttaa tccaccagac gatattcacc
    15841 cgtagcggtc atcattaagg tcaaatttgc aggcagactc tcttccagca cccggcgaac
    15901 attcggcccg tcggtacggt cataaaaggc ttctgcatct gccagcgata cccctcccgc
    15961 cagcttgcgc cccaccagac gctcccgcaa cgcagcagct ggcgcgttga taaagatcga
    16021 gaaatcacaa aattgcgcaa gctggcacca cttctcgtcg tccagcaaca accagtttcc
    16081 ttcgacgatg acgagtggtg cggtaacgtg caacgcatct tcaacaggat catgcttttg
    16141 tcgatcgtac tgcggccacg tacaatcccc ttccaccacc tggcgcagat tttccgccag
    16201 tttcgcaacg tcaaatgtct ctggtgcgcc tttgaagggg cgcaattgat gcgcgtccag
    16261 ccagctattg taatggtgaa aaccatccat cgggagcgtc tgaatagcgg gcagctccgg
    16321 gtcttgttgc gcgagatatt cccagaaggt ggttagggta gatttacccg tccccggcgg
    16381 agcgcaaaga aaaaccaccg tacgccgctg cggatttacc gtctgtaagg ccgccagcat
    16441 acgcagcaac ggtttatgca cgcgttctat ctcttcatca tggtactgcg cttgcacgtt
    16501 cagcccattc accgttaatt caattttcac ggcgttaatt cctttaaggt ggtcgttact
    16561 gccagcgtca gcgccgtttt gaccattccg ctaaaacgcg cctctgacca gacggcgaaa
    16621 tcggcgaact tcatcgcttt taacgcatcg tagagcgtcg cattgcgagt tatactgttg
    16681 acattgctga taaaatccca ggttatatca tcggcaaatc cgggcgtttc atcctgttcg
    16741 ttcaggtaat gccagtactg ggaaaagtgc gtgatatcgg catacagcca tttatccatt
    16801 ttccccagcg cataaatcaa acgcagcgca acatcaatat cgtcaagcgg gccgctttgg
    16861 gcgagtaacg gcttcactgc atattcgacg atctcttcat cgttatcgac aaacagcgac
    16921 ggcagaaact ggcgtatccc ctggagcaaa atagaatgcg cagttttcat aaatgaaaat
    16981 aaattgtcct gtgcatccag ttgctcaagc acatcatctt ctgtcagagt cgccattggt
    17041 cttccgatag atcaccatca atgcagggag tctattatat tacacgtttg gtccgcgccg
    17101 ccgatcagta atgtctgcgt ccgcacccca ttcgccgtct gttggatacc gttcaccaac
    17161 tcgccgcccg tcacgcccgt agcgctaaag aggatatcgt tccccctcac cagttcatcg
    17221 agcgagtaca cacggttaac gtcaacgccc attgccttac aacgcttgcg ctcctgctct
    17281 gcaatctgcc gattttccgt gtaatcccct ttagcctgac aaaagtcaat cagctcggcc
    17341 tgcatatccc cgcctaacgc tttaacggca caggcagaaa tcacgccttc tggcgcaccg
    17401 ccgatggtgt acatcacatc gtaaggatta tcctgccagc aggtcagcac gctggcggcg
    17461 acatcgccat ccggcagggc aaaaaccttc acgcccagct gtgttgcttc ttcaatcgcg
    17521 gcactcagac gcggtttatc cagcgtaacc atgcgcagct tgtccagcgg tttacctaac
    17581 gcccgcgcca cgttacgcaa gttgtccgcc agaggcaaag acagatcaat cgccccggca
    17641 gccagtcgat taacaaccag ctttttcata tacatatcgg gcgcatgtaa caggctatct
    17701 cgaggagcga aagccatgac cgccagcgca ttgctctgcc ccatcgccac catccgcgtg
    17761 ccttcaatgg gatcaaccgc gatatcgact tctggcccat cgcctttgcc cacctcttcg
    17821 ccaatccaca gcatcggcgc atggtcaatt tctccttcgc agataaccac tcgtccacga
    17881 aaagcgacgt cgtttaatgc ctggcgcatc gcggtgaccg ccaggccatc aattttattt
    17941 ttgtcgccac atccggtttg cggccaggca gccaacgcgg cctgttccgt aacgcgaaat
    18001 aatggccacg ccagggacat cattgcgcgg cctccccgat atcaacgcca aattgtgcca
    18061 gcagatattt ctcggcctgt tcattccaga tgccgtgcgt ttcctcaacc agccgcgcca
    18121 actctttaaa cagcggatcg gttttgcctt tttcggcaaa atcggcaatg gcagtgagcg
    18181 gcatggtcac gccgttataa atgagttttt taccgccagg gatatccggc agattgagca
    18241 cggtttctgg caccgcatcc aggccaccaa tatgggtcac cataaacgac ggctgtaact
    18301 gtcctgtggc gctaagggca atcgcctctt tcatgtcgtc cgttgaaccg ccggatgtac
    18361 cgactacgtg cgtgctgttg taatggacgt tgtagaaatt aaacggcact ttgaagtttt
    18421 tatccgtcag cccggcaaag aagttcagac agccatcttc cgccagtaat tcgtcagcca
    18481 tctcaacgac agcaggcacc gccgcataaa caaaaatgtc atcgaacccg acatctcctg
    18541 tcagcgcccg cagcatctgg ataggatcgc tcatcccttt ggtattcaca tacaccagct
    18601 caatgccttt actggccgcc agatccaccg gcagcagctt ctgcacctgc gccaggcgtt
    18661 tgtcgtcgat atcgaccacc accacccgtg acggttgtat gccaccgtta atggcgtaat
    18721 cgatagcgcc aatgcccatc ggacccgcac acgccagcag tgcaatattg ccgccaggtt
    18781 tgacgcccat gcgatgctca taaacatatt gcgtggtgtg ataattggca tgataagcac
    18841 caataatgca gcacataggc tctgccagcg acgccgcagc aaaataagag ccgtgatacg
    18901 gtaatacgca gcccaaatta atggcgattt cgggaataat catataagtg gcattgccgc
    18961 caaaatattc gtagctgtag cccgctgaat atccgcttgg taaccccatc gccggttgca
    19021 atacaaaacg ctggcctttt ttatatttgc cagtgagatt tttacccact tcgacaataa
    19081 tcccggcaca ttcatgcccg gtaatgaccg gatgattttc taaatcgtcg ggtacgcgtt
    19141 tatgttcact accgagtaac gccgctttcc aggtcgataa acagacgctg tcagaaatta
    19201 cactcactaa cagttcatta tcggtaattt ctggcagttc aaat
//
LOCUS       DAJTRQ010000073        19095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000073 DAJTRQ010000000
VERSION     DAJTRQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(47..139)
                     /locus_tag="OO332_002574"
     tRNA            complement(47..139)
                     /locus_tag="OO332_002574"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO332_002575"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO332_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5943008.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO332_002576"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO332_002576"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5943009.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO332_002577"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO332_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5943010.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO332_002578"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO332_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5943011.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO332_002579"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO332_002579"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5943012.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO332_002580"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO332_002580"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5943013.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8063
                     /gene="srlA"
                     /locus_tag="OO332_002581"
                     /gene_synonym="gutA"
     CDS             7500..8063
                     /gene="srlA"
                     /locus_tag="OO332_002581"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5943014.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8060..9019
                     /gene="srlE"
                     /locus_tag="OO332_002582"
                     /gene_synonym="gutE"
     CDS             8060..9019
                     /gene="srlE"
                     /locus_tag="OO332_002582"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5943015.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9030..9401
                     /gene="srlB"
                     /locus_tag="OO332_002583"
                     /gene_synonym="gutB"
     CDS             9030..9401
                     /gene="srlB"
                     /locus_tag="OO332_002583"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5943016.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9405..10184
                     /gene="srlD"
                     /locus_tag="OO332_002584"
     CDS             9405..10184
                     /gene="srlD"
                     /locus_tag="OO332_002584"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5943017.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10289..10648
                     /gene="gutM"
                     /locus_tag="OO332_002585"
     CDS             10289..10648
                     /gene="gutM"
                     /locus_tag="OO332_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5943018.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10715..11488
                     /gene="srlR"
                     /locus_tag="OO332_002586"
                     /gene_synonym="gutR"
     CDS             10715..11488
                     /gene="srlR"
                     /locus_tag="OO332_002586"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5943019.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11481..12446
                     /gene="gutQ"
                     /locus_tag="OO332_002587"
     CDS             11481..12446
                     /gene="gutQ"
                     /locus_tag="OO332_002587"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5943020.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO332_002588"
     CDS             complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO332_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5943021.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14144..15583
                     /gene="norV"
                     /locus_tag="OO332_002589"
     CDS             14144..15583
                     /gene="norV"
                     /locus_tag="OO332_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5943022.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15580..16713
                     /gene="norW"
                     /locus_tag="OO332_002590"
     CDS             15580..16713
                     /gene="norW"
                     /locus_tag="OO332_002590"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5943023.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO332_002591"
     CDS             complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO332_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5943024.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
BASE COUNT         4486 a         5310 c         4884 g         4415 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
     7381 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     7861 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7921 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
     7981 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8041 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8101 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
     8161 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8221 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8281 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8341 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8401 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8461 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8521 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
     8581 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8641 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8701 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
     8761 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8821 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8881 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8941 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9001 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9061 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9121 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9181 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
     9241 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9301 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9361 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9421 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9481 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9541 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9601 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9661 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9721 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9781 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9841 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9901 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
     9961 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10021 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10081 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10141 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10201 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    10261 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    10321 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    10381 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    10441 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    10501 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10561 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10621 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10681 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10741 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10801 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10861 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    10921 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    10981 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    11041 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    11101 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11161 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11221 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    11281 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    11341 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    11401 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    11461 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11521 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    11581 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11641 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    11701 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11761 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    11821 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    11881 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    11941 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    12001 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    12061 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    12121 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    12181 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    12241 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    12301 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    12361 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12421 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    12481 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    12541 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12601 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12661 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    12721 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    12781 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    12841 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    12901 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    12961 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    13021 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13081 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    13141 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    13201 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13261 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    13321 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    13381 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    13441 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    13501 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    13561 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    13621 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    13681 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    13741 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    13801 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13861 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    13921 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    13981 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    14041 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    14101 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14161 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    14221 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14281 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14341 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    14401 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    14461 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    14521 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    14581 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14641 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14701 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14761 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14821 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    14881 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    14941 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    15001 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15061 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15121 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    15181 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    15241 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    15301 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    15361 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    15421 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15481 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    15541 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15601 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    15661 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    15721 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    15781 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    15841 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    15901 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    15961 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    16021 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16081 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    16141 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    16201 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    16261 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    16321 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16381 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    16441 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16501 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16561 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    16621 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16681 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    16741 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    16801 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    16861 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    16921 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    16981 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    17041 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17101 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    17161 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    17221 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17281 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    17341 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    17401 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17461 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    17521 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    17581 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    17641 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    17701 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    17761 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17821 cgtcacttaa atcgcccaga tgctgactca acaccacttg ttcaccgcgc accaggcaga
    17881 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    17941 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18001 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    18061 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    18121 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18181 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18241 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    18301 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    18361 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18421 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18481 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    18541 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    18601 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18661 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    18721 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    18781 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    18841 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    18901 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    18961 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    19021 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    19081 tgttttttgc cattg
//
LOCUS       DAJTRQ010000074        18678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000074 DAJTRQ010000000
VERSION     DAJTRQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..507)
                     /locus_tag="OO332_002592"
     CDS             complement(<1..507)
                     /locus_tag="OO332_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5943025.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGI"
     gene            complement(507..1748)
                     /gene="clsB"
                     /locus_tag="OO332_002593"
     CDS             complement(507..1748)
                     /gene="clsB"
                     /locus_tag="OO332_002593"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5943026.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(1745..2506)
                     /locus_tag="OO332_002594"
     CDS             complement(1745..2506)
                     /locus_tag="OO332_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5943027.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            2639..3049
                     /locus_tag="OO332_002595"
     CDS             2639..3049
                     /locus_tag="OO332_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5943028.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3011..4117)
                     /locus_tag="OO332_002596"
     CDS             complement(3011..4117)
                     /locus_tag="OO332_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5943029.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4128..5261)
                     /locus_tag="OO332_002597"
     CDS             complement(4128..5261)
                     /locus_tag="OO332_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5943030.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(5254..6990)
                     /locus_tag="OO332_002598"
     CDS             complement(5254..6990)
                     /locus_tag="OO332_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5943031.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(6983..7978)
                     /gene="hlyD"
                     /locus_tag="OO332_002599"
     CDS             complement(6983..7978)
                     /gene="hlyD"
                     /locus_tag="OO332_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5943032.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(7981..8652)
                     /gene="cecR"
                     /locus_tag="OO332_002600"
     CDS             complement(7981..8652)
                     /gene="cecR"
                     /locus_tag="OO332_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5943033.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            8881..10068
                     /gene="rhlE"
                     /locus_tag="OO332_002601"
                     /pseudo
     CDS             8881..10068
                     /gene="rhlE"
                     /locus_tag="OO332_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(10275..10757)
                     /gene="ybiA"
                     /locus_tag="OO332_002602"
     CDS             complement(10275..10757)
                     /gene="ybiA"
                     /locus_tag="OO332_002602"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5943034.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            10877..13027
                     /gene="dinG"
                     /locus_tag="OO332_002603"
     CDS             10877..13027
                     /gene="dinG"
                     /locus_tag="OO332_002603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5943035.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13055..14017
                     /gene="ybiB"
                     /locus_tag="OO332_002604"
     CDS             13055..14017
                     /gene="ybiB"
                     /locus_tag="OO332_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5943036.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            14158..15243
                     /gene="hcxB"
                     /locus_tag="OO332_002605"
     CDS             14158..15243
                     /gene="hcxB"
                     /locus_tag="OO332_002605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5943037.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(15472..15732)
                     /locus_tag="OO332_002606"
     CDS             complement(15472..15732)
                     /locus_tag="OO332_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5943038.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(15997..16263)
                     /gene="ybiI"
                     /locus_tag="OO332_002607"
     CDS             complement(15997..16263)
                     /gene="ybiI"
                     /locus_tag="OO332_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5943039.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(16337..17014)
                     /gene="ybiX"
                     /locus_tag="OO332_002608"
     CDS             complement(16337..17014)
                     /gene="ybiX"
                     /locus_tag="OO332_002608"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5943040.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17056..18678)
                     /locus_tag="OO332_002609"
                     /pseudo
     CDS             complement(17056..18678)
                     /locus_tag="OO332_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4306 a         4918 c         5076 g         4378 t
ORIGIN      
        1 aatgccgaga atacgcagcg tagtttgatc gacataccag tgatccggca actccaccac
       61 gcctgcggta aagataatcc ctgccagtaa aatatagccc agccagttgg tggtaatact
      121 gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct agtcgggagt acaaacgata
      181 gcgcatacca atgccgccga cccaggtact gagcgtcagg ttgaaggcgt agcagataaa
      241 cgacaccagc atcacctggc gcttcgccag tttgtgaccg cagtaaaagc gggcgagcag
      301 gtcatagcag ccgtaaatca gatagctgac gaccaccagc ccgaccgcac taagcagcgc
      361 aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt ttttggcgta
      421 gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct tctttgctaa
      481 gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc agtttctacc
      541 cgatcctgcg tttccattgt cggttgtgcg gggggatcaa cctgcgtcag acgtggtgtg
      601 tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa cgccagcacg
      661 cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac ctgctgacaa
      721 tctgcggcaa taatgccgtt cagattatcg cgcagcgtct ggttaaaatg acgatcgtgg
      781 atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt actggaccct
      841 actgtcgccc agtgatcgtc catcaatgcc actttgccgt ggagcgggcg gcggcggtac
      901 tcaaaaacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc accgactctg
      961 acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc gcgccgtgcc
     1021 gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt ggcgataatc
     1081 acttcccgcc gcgcctgagt gagcattttc agataatggc gttcaatatc atcgcgatgt
     1141 tcttcgttat cgcgccagac cagcaatacc tgcgcttctc ccggctggcg gttctcttcc
     1201 gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag gttttccagc
     1261 tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta atcctgttta
     1321 gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc aataaaggcg
     1381 atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt ggtgcgcata
     1441 ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc cgtcagttca
     1501 ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac ttccgcttta
     1561 accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac gttatcctca
     1621 aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc cttaaacacc
     1681 gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc gcgccagcta
     1741 catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt cgccatgtcc
     1801 gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc ctgtccagtc
     1861 gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg cgggtaaaaa
     1921 tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc cagtcattga
     1981 aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat tcggcgagca
     2041 tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac atcacatgaa
     2101 tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc ttttccgcac
     2161 catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac agtacggcgt
     2221 tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg ctccacatag
     2281 tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc agcggatgaa
     2341 cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg gtacgcacgg
     2401 catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct ttgtgaatgt
     2461 tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt ttcctgtctt
     2521 tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg tcttacggaa
     2581 ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga gagaagccat
     2641 gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga ttatgttgca
     2701 tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg ttcacgtcgg
     2761 cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt ttcagcgcca
     2821 ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca cgacctggta
     2881 ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa acaacttttt
     2941 gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt tgctggcgaa
     3001 agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac cctgtcgtgg
     3061 ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc gcatccttca
     3121 aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac gtcagatttt
     3181 gcagccacac tggcatgttt tccaccggcg aaacgtaacc ggaaaggaga atggcgggca
     3241 tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt gatgaaatca
     3301 acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag aacagcgcta
     3361 gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca atggtggcct
     3421 ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc caggtggtga
     3481 gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg acggaaagtg
     3541 aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc accacaaacc
     3601 atttgtagtc gagattcggg ttataccagt tgcgtaccac cagctcgctg ttgttgggtt
     3661 tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc aggtagttgg
     3721 cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac ggcgcggtct
     3781 ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt aacgcctttt
     3841 gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag cagcagcaca tgtgtaaagg
     3901 cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg ctcgccgtta tcttcatcgt
     3961 agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac aggatcacct
     4021 gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc aacgactgca
     4081 actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta atccagccga
     4141 cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat caaaaacagc
     4201 acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg cagggtgctg
     4261 acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg catactgtcg
     4321 atctgaaaaa taaagccgga aagcataatc gacggcagaa aagcggcgtt cagggcgacc
     4381 tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat tcccagggta
     4441 ctgagtaaaa acaggctgga gataaaaaac agaatcagca gcgaaccgcg atacggcacg
     4501 ccgagaataa acactgacac cagcatacac agcaacatcg ccagcatccc gagaaagtaa
     4561 taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga gagcagagcc
     4621 tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat cgcgccgatg
     4681 accgtcatga taatggtcac cgcacccggg ataataaagt gctggcttat cgccgccggg
     4741 ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc gttgtcctcc
     4801 gctcgctgca tttgccagat ctgccagatc ccttcgacat acccctgcac aaagttagcg
     4861 gtattcggct cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt ggcgcgctcc
     4921 atctgttcgg cgaaatccac gggaataacc accagaccgc gaattttccc cgactgcatt
     4981 ttggcgatca gttcctgacg gttatcgctg atggtggcgt cgatgtaggg cgaaccggtc
     5041 atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt ccagcaaaat cccgacccgc
     5101 agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa acagcagtag cagtgggatc
     5161 actaccgcaa tcagccagct actcggatcg cgaacgatct gccgcgtctc tttgacgcac
     5221 agcgcccgca cgcgatgcca ggaaaggatc gggttactca ttgctatgct ccttatccca
     5281 gtcgtggatc aactgaataa aggcttgctc catggtggga tcgggttgct catcgttagc
     5341 tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt aatttcccac ggtacaccag
     5401 gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg gtggtgacca tcaccgtgac
     5461 gcctttctct accatgctgt tgatgtgcag ccaaaattca cggcgggtga gggggtcaac
     5521 gccggaagtc ggttcgtcga gaaagagaat atccggttca tgcatcagcg aacaggccag
     5581 cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg gcgtgtgagg caatactttt
     5641 caggccaaat gcctcgctca tacgggagat tttttcgttc tgcgcccgac cgcgtaagcc
     5701 atacacacca gagaaaaagc gtaaattctg ttcgaccgtc aggttgccgt agagcgaaaa
     5761 tttttgcgcc atatagccga gatgctggcg cgctttaccg gaactctctt tcagatccat
     5821 ccccagcacc agcgcctggc cggaagtcgg caccagcaaa ccgcacatca tcttaaaggt
     5881 ggtcgattta cccgcgccgt ttggccccag caaaccaaaa atctccccac gtttaacggc
     5941 aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt ctttcgcttc
     6001 gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat attgcaccca gcggcgattc
     6061 tgaggttccg gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg gcgtagtttc
     6121 gttgatgttg atttccggca tcccgtcggc atggcgaata tcgtctggtg tggcctcttt
     6181 tttgaggatc agacgtaccg atttcccctg aatcatgccg tcgctgacct gcggcagttt
     6241 caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga ctggtcatca gaaagctgcg
     6301 tccggccatg gtttgtgtca gggcttttgg ttctccctga tacagcaact cgccttcgtt
     6361 catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac tccagaggat
     6421 taacatccct tcgcccgcca gctcatgcac catctgccac agttcgcgcc gtgagatagg
     6481 gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca ccagggtaca
     6541 ggccaggccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc gtccggtaaa
     6601 cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg cctcgccggt
     6661 gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat cttcatacag
     6721 accaaatttc tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt ttttgatcgg
     6781 atcaaagcca atcaccgtgg cactgccgct gtcgggtttc agtaatcccg ccaacatccg
     6841 catcagcgtg gttttacctg caccgtccgg ccccaccaac cccgtcacat aaccggcgtg
     6901 aatggtacaa tcgagcggcg cgacggcggg cttgtccatg cccggaaagc gtttttccag
     6961 gccgttcagc gtgataacgg catcattcat gtcctgcctc gtcaccgaat tgtaccgtca
     7021 ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc aggcgataga
     7081 cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg ggtaaattca gcagtcggcg
     7141 aaacgaaacc aatctgcccg tgatacggct tgtccgggcg accatcggta taaagcagca
     7201 ctttgcgccc cggctgggcc tggtcaagat tccgttcatc aacataagcg cgcacccaca
     7261 ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgagaacc gtgcctggct
     7321 ctaccgcgcg cgttaacagc gtgccatcag acggtgctat caacgttgag tcctgtaaat
     7381 tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc gcctgagcga
     7441 tatcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct gatttcagcg
     7501 ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc gaaatggtgc
     7561 ggcttttcca cagtccttgc tggcggttat agaagttctg cgcatagtca taggcggctt
     7621 gcgcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac ccggcaagca
     7681 tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc agggcaatct
     7741 catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca tcaccttcgt
     7801 ccaccgccag cgattcaacg cgccccccaa cacggaaact aagattcacc gtacgaatat
     7861 ccacgttgcc atacagcgtc aggccgttat cttggcggct ttgataccac cagtagcctc
     7921 cggcaaccac ggcggcaagt accactaccg ccagtccgat cacgacaggt tttttcatca
     7981 ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg tcaccgtctg
     8041 gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta acagaattgt
     8101 ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgggaat gaaggatcat
     8161 gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc aggcgtgtca ggtggctgtg
     8221 tagcggacta atcacctgtt cgtgcaccag gtggtaggct gccgtgggag agagctgctc
     8281 acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct taatcatgtt
     8341 cctgcaggcg cgaaggatca gttcacggat ggcagcccga tcaggctgtg gttgggcgaa
     8401 caagcgttcg gcttcctcgg catgcggacg gaactgctcg ccaataaaat cggcaatcca
     8461 ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga tggcagcgat
     8521 attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg ttcattccat attcaccaaa
     8581 ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga ttgtcatggc
     8641 aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt atgactccat
     8701 atgggagttg tccagtatgg ctaagaattt tagctacgcc agtcacaggg ataatttatg
     8761 cgctgcgtca caaaaactgc tacactccgc tccctcatga cattgtggtt tttgtcattt
     8821 tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt cggagtagtt
     8881 atgtctttcg attctttggg tttaagccct gatatcctgc gcgccgttgc cgagcagggt
     8941 taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga aggccgcgac
     9001 ctgatggcta gcgcccagac cggcaccggc aaaacagcgg gctttacgct gccgctgttg
     9061 caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg tgcgctcatt
     9121 cttaccccga cccgtgaact ggcggcacag attggcgaaa acgtccgtga ttacagcaaa
     9181 tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca gcattaaccc acaaatgatg
     9241 aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg gacgtttgct ggacctggaa
     9301 catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga agctgaccgc
     9361 atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact gccagcgaaa
     9421 cgccagaacc tgctgttctc tgcgaccttc tctgacgata ttaaagccct ggcggaaaaa
     9481 ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc tgatcaggtg
     9541 acccagcacg ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc gcacatgatt
     9601 ggtaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg cgctaaccat
     9661 ctggctgagc agctcaataa agatggcatc cgtagtgtgg cgatccacgg gaataaatcg
     9721 caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg gtgacattcg cgtactggtg
     9781 gcgaccgata tcgctgcgcg cggtctggat atcgaagagt tgccgcacgt cgtcaactat
     9841 gaactgccaa acgtaccgga agattatgtc caccgtatcg ggcgtaccgg tcgtgcggct
     9901 gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct gcgtgatatc
     9961 gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga gccggatccg
    10021 tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcat caggctgatg
    10081 aacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggac tctgcaatat
    10141 attgaatttg catgcttttg taggccggat aaggcgttta cgccgcatcc ggcatttcac
    10201 aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt taccgcccat
    10261 aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat taaaaggtag
    10321 cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc gttttccgta
    10381 tgctcaacca gttttgcggg cgcggttgcc agcaagagca cacgcagttc tgcatgctgt
    10441 tcgaatttag cgcgaagtgc tttacgcatc acctgttctt taaccgactc ccagttttta
    10501 cgaagtggct tggaacgatt acgccccatg cgagcggcga ccatggggga agaaacccga
    10561 cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata gtgttcggag
    10621 gtgggccagg tattcccgtc aaccttgatg ggcctggcgg caaagttaga aaaatcaccg
    10681 tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg ttggatcctt
    10741 tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc gctttccaga
    10801 aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct gtttatacag
    10861 tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg ccgcctggta
    10921 taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc ggcagatgat
    10981 tgcggacgtc gccaaaacgc tggccggaga agaagggcgg catctggcga ttgaagcccc
    11041 caccggcgtt gggaaaacgc tctcctattt gatccccggc atcgccattg cccgcgaaga
    11101 gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg caggatcaga tttacagcaa
    11161 agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg ctttcgggcg
    11221 tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc agcactgaac ccacgcaaca
    11281 ggatctgctg gcgtttcttg acgacgaact gacgccaaat aatcaggaag agcaaaaacg
    11341 ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg gatggtctgc gcgatcatac
    11401 ggatatcgct atagatgatg atctctggcg tcgtttgagt accgacaaag ccagctgcct
    11461 caaccgtaac tgttactact atcgtgaatg cccgtttttt gttactcgtc gggagattca
    11521 ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg aaagcgaagc
    11581 cgtactgcct gacccgaaaa atctgctgct ggtgctggac gaaggtcatc acctgccaga
    11641 tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc gcgccctggt atcgactaca
    11701 gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc cgaagaccat
    11761 cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt atgagcttat
    11821 cgcctcgtta aacaacattc tcaatctcta catgcctgcc gggcaggagg cagagcaccg
    11881 ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc tgccagcggc tggcaaaact
    11941 caccgagatg ctgcgtggtc tggcggagtt atttcttaac gatttaagtg agaaaaccgg
    12001 cagccacgat attgtgcgtc tgcatcggtt gattttacag atgaaccgcg cgctggggat
    12061 gtttgaggtg caaagcaaac tctggcggct ggcgtctctg gcgcaatctt ccggtgcgcc
    12121 ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctacacctct ggtttcactg
    12181 cgtgggaata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta ttccgcacat
    12241 tattgtcacc tccgcaacct tgcgttcgct gaatagtttt tcgcgtttgc aggagatgag
    12301 tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct ttaaccactg
    12361 cgaacagggc aaaattgtta ttccccggat gcgctttgag ccttccatcg acaacgaaga
    12421 gcaacacatt gccgaaatgg cggccttttt ccgtgaacag gtggagagca aaaaacatct
    12481 cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg actatgtgac
    12541 ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg cgttatcgct tagttgaact
    12601 gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg gtgggcttac agtcatttgc
    12661 cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca aaatcgcttt
    12721 tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa gcctcaaccg
    12781 ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc agcaggttgg
    12841 gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca aacgcttgct
    12901 gacaaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga tagagcaacc
    12961 ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac gccgtcggcg
    13021 gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa taattaaaga
    13081 gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc gcggtctgta
    13141 tgctcatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat tgattgcgct
    13201 gcgtatcaaa ggggaagggg aagcagagat gctcggcttt tacgaagcca tgcaaaatca
    13261 caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg attgtcatcc ccagttacaa
    13321 cggcgcgcgt aaacaggcta acctgacgcc attattggcg attctgctgc ataaactcgg
    13381 ttttcctgtg gtggttcacg gagttagcga agatccaact cgcgtgctga cagaaaccat
    13441 tttcgaactg atgggtatta caccaacgtt gcacggcggg caggcgcagg cgaagctcga
    13501 cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc ccgccgctgg aaaaacaact
    13561 ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga aactggcgac
    13621 gccatttgcc gaaggtgagg cactgtgtct ttccagtgtt tcgcatccgg aatacattgg
    13681 acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg ttactaatgc atggtactga
    13741 aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg accgtgaagg
    13801 gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc gagttactgc cacaagcaaa
    13861 agatccggaa accacggcgc agtggactga gcgttgcctg gctggcagcg aagcgattcc
    13921 cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag cggcaactat
    13981 cagcgatggc ctggcgcgcg ttaatcaggc attttaattt ttatctcagc cggatgagcc
    14041 acgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat tgaccttccc
    14101 gcgcccggcg tgcagcataa aaaaacaaca aacacataac ataaacagga gttaaccatg
    14161 gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc tgtatttcgt
    14221 cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat cgcggcaaac
    14281 ctggcagggc atgattctca tggtattggc atgatcccaa gctatgtgcg ttcctggagt
    14341 caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta aagaggcggg ggcggcagtc
    14401 acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac acgaagcgat ggcgctgggg
    14461 attgagaaag cgcatcagca cggtattgct gccgtggcgc tacataactc gcatcatatc
    14521 ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg ggtttgtctc tatccacttt
    14581 gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag ccgctttggc
    14641 accaatccct tctgtgtggt tttccctcgt aaagataatt tcccgctgtt gcttgattac
    14701 gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg cgtccccgtg
    14761 ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc ggtaatgcag
    14821 gagtcgccgt tggggtcgct gttgaccttt gccgaacata aaggctacgc ccttgcagcg
    14881 atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa cgacgcataa ggaaacgttg
    14941 caaaccagcc ccgatgccat tcttaactgc atgaccacta tcatcatcaa cccggaactg
    15001 ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt gaaagcctcg
    15061 ccgcatgatg acgataagcc gattttgcta ccgggcgagt gggaagtgaa cacgcgtcgc
    15121 gaacggcagg agcaggggat tccattggat gcgggaagct ggcaggccat ttgtgatgcg
    15181 gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt tctgtcagca gttagccagc
    15241 taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga tcaatgaggg
    15301 ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg acgtagcgta
    15361 aacgcctgat ccgtccaacg gttcggattt tgtaggcctg ataagacgcg gtagcgtcgc
    15421 atcaggcatc tgttgccgga tgcggcataa acgccttatc cgtcctacga attacttata
    15481 aattaccgca gtaccgctta atttattgtt gttggtggcg gaagtgatgc tgtatccact
    15541 ggcaccggct gccgccgctt tctcagccag tttcgcttcc agggcatcga gggtggatgc
    15601 gccatcggca gaaaccacgc cgattttatt catgttctgt gcctgggatg ccgttaccgg
    15661 ttccgccgcg aacacgccaa atgacagagt tgaaagagcc atagcagcaa caacagtatt
    15721 gatagttttc ataattaatc tctcgcaggt gatttttgta taagtgacgt tgtttcgtcg
    15781 atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg accgccttgt
    15841 tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg tacttatgga
    15901 cttgttttat gccgcgtggc agtcacagca gcgttaaagg cacggaatgc cacgcaaaat
    15961 acaaattata ctcgctatgc atctcgccag ttgtagttaa cgtaactggc tgtctttcga
    16021 acctctgcga ttatatcctg tatacgctgg tttttgtaaa tctttctcct gctgacaatg
    16081 aatacataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg gggcaccgca
    16141 ctcttcacat tcatacaggc tttcgccacg cggaatttca ccccgagcgc gggcaatcgc
    16201 atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc aagcggatgc
    16261 catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatat agggacgaac
    16321 ctgggcagtt caagcgtcag atctccgacc attcccgcag cagattatga taaagattga
    16381 gcagcgacag gatctcttca ttttcaccat agcggctttt cagcgactga atatttttgt
    16441 ccagttcaaa cagcatggcg cgctttttat catcgcggat catcgactgg atccacataa
    16501 atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg ctggagggat
    16561 acaacaccag atcacctgcc gggagtttta cccgatgttg tccaaaggtg tcattaacga
    16621 ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca gaaaggtcag
    16681 tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatgg aaaccatagg
    16741 tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc aaggcagcgg
    16801 caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc attttgcagt gcggcgtata
    16861 acgcgctgcg ggtgtctact tgctgattgt tcttaacttg tgcaccttgt gctccggtgg
    16921 tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaatgg gcgacgtcct
    16981 gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa gtggggcctg
    17041 cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc ttggctcgcc
    17101 cgggtgataa cggtagccgc tcttgttgat tgaggcgacg taatcggtat caaacaggtt
    17161 gtaaacgttt agctggaagt cgagattgcg attaacccga taccccagtt tggcatctgc
    17221 gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg ccgtctgaac ctttatgtat
    17281 actgccgata tagcgtgcgc ccgcgccaac agagatatca tcggttgcct gatattggct
    17341 ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat cctgggcaac
    17401 atctttgccg tttttgatgg ttgctttttt ctgggtatag ccgccaatca cctgccacgc
    17461 gggagtgata ttccctgcca cggatatctc atagccttcg acgcgtttct taccgtattg
    17521 cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg atatcagtac ggaacagcgc
    17581 ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct cgctggtgtt
    17641 ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccactgccag actgcgcaag
    17701 ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat agacattgcc
    17761 gttttccgtc aggtgataca gcgccccggc tttccagttc accagattgc ccgacttggc
    17821 ggtgtcgacg gtggtgactg gagaaccttt tgccacacca gctgggcagg tgatggcacc
    17881 gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat tatccagacg
    17941 gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg cgtaaattgc
    18001 gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc cgggatgaat
    18061 tctgctgtca ggatgataaa tatttaccgc cggtaacgtc accggattaa cgccatagtt
    18121 agtctgcgtc tcacgggtaa attccacgcc ggtactgaca tcatgaccga tagaaccggt
    18181 atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca catctttggt
    18241 attcgccgtg cgtgactagg tccagctatt cacatcgctg gtgggctgag taatattcga
    18301 cgccccgccc ataatcgccg tcatcaggta atcctgcttt acgcgcgacc aacgggtagt
    18361 attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg cggtgtcggt
    18421 ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa cttttccgga
    18481 atgattcagg gccgccgctc ccgcagatgg ggcagaatag cccggcaaac cgatggtcgg
    18541 aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga taattaagat acaaacgatt
    18601 cgctgtacca aggccaaaag agacagaagg ggcgacgccg taacgctcat ttttgacttt
    18661 gtcgcgtccg gcatcgtg
//
LOCUS       DAJTRQ010000075        18161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000075 DAJTRQ010000000
VERSION     DAJTRQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..720)
                     /gene="aroD"
                     /locus_tag="OO332_002610"
     CDS             complement(<1..720)
                     /gene="aroD"
                     /locus_tag="OO332_002610"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5943041.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVN"
     gene            complement(751..1617)
                     /gene="ydiB"
                     /locus_tag="OO332_002611"
     CDS             complement(751..1617)
                     /gene="ydiB"
                     /locus_tag="OO332_002611"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5943042.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1629..2900)
                     /locus_tag="OO332_002612"
     CDS             complement(1629..2900)
                     /locus_tag="OO332_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943043.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3083..4287)
                     /locus_tag="OO332_002613"
                     /pseudo
     CDS             complement(3083..4287)
                     /locus_tag="OO332_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4387..4743)
                     /locus_tag="OO332_002614"
                     /pseudo
     CDS             complement(4387..4743)
                     /locus_tag="OO332_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4879..4986)
                     /gene="rprA"
                     /locus_tag="OO332_002615"
     ncRNA           complement(4879..4986)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO332_002615"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5172..6284)
                     /gene="ydiK"
                     /locus_tag="OO332_002616"
     CDS             complement(5172..6284)
                     /gene="ydiK"
                     /locus_tag="OO332_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5943044.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6673..9729
                     /locus_tag="OO332_002617"
     CDS             6673..9729
                     /locus_tag="OO332_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5943045.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9726..10136
                     /gene="menI"
                     /locus_tag="OO332_002618"
     CDS             9726..10136
                     /gene="menI"
                     /locus_tag="OO332_002618"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5943046.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10236..10424
                     /locus_tag="OO332_002619"
     CDS             10236..10424
                     /locus_tag="OO332_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5943047.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10972..11340
                     /gene="sufA"
                     /locus_tag="OO332_002620"
     CDS             10972..11340
                     /gene="sufA"
                     /locus_tag="OO332_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5943048.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11349..12836
                     /gene="sufB"
                     /locus_tag="OO332_002621"
     CDS             11349..12836
                     /gene="sufB"
                     /locus_tag="OO332_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5943049.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12846..13592
                     /gene="sufC"
                     /locus_tag="OO332_002622"
     CDS             12846..13592
                     /gene="sufC"
                     /locus_tag="OO332_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5943050.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13567..14838
                     /gene="sufD"
                     /locus_tag="OO332_002623"
     CDS             13567..14838
                     /gene="sufD"
                     /locus_tag="OO332_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5943051.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14835..16055
                     /gene="sufS"
                     /locus_tag="OO332_002624"
     CDS             14835..16055
                     /gene="sufS"
                     /locus_tag="OO332_002624"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5943052.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16068..16484
                     /gene="sufE"
                     /locus_tag="OO332_002625"
     CDS             16068..16484
                     /gene="sufE"
                     /locus_tag="OO332_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5943053.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16633..17637
                     /gene="ldtE"
                     /locus_tag="OO332_002626"
     CDS             16633..17637
                     /gene="ldtE"
                     /locus_tag="OO332_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5943054.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17701..17937)
                     /gene="lpp"
                     /locus_tag="OO332_002627"
     CDS             complement(17701..17937)
                     /gene="lpp"
                     /locus_tag="OO332_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5943055.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4780 a         4387 c         4667 g         4327 t
ORIGIN      
        1 atttaccgag atttgccctg gcgcagaggc tttttttacc gcaccaaaag ttgccgccga
       61 gccaaatact tcaccagcca gacgagaaat tacgccagtt tttgccatcg acatcgtgat
      121 gattggacga tcggcatact gctcttgcat ctccagggtc gcggcaagca acgtcagcac
      181 atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcgtcga aggactgcat
      241 tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt catggttgga
      301 catgacaact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc
      361 gccggtaaat aactccagat cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt
      421 gagagcaata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa
      481 ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgccg ccgccatgac
      541 agactccaca ttggagaggt cggcaaagtg gtccacacgc cattccagaa tatcaaagtc
      601 cgcttcacga taggcgagag cttcggattt cacgcgggcg atatctttcg ccatcagcga
      661 gacgatgatt ttaggtgcgc ccgcaccaat gacgagatct tttacagtta cggttttcat
      721 tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga cctgtttaac
      781 atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc cttgccacaa
      841 caacatgccg tatccatcaa tcgttttgca accagcttgt tgtgcctgct gcaataactt
      901 cgtcatatgc gggttataca cgcattcagt gaccagcagt cccggatgta acagactgat
      961 atcattaacc aatgattcat tctcaagggg tttcatacct acttttgtgc cattggttaa
     1021 aatgtcggcg gaagccaggg cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt
     1081 gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa
     1141 ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc
     1201 aatcgccgtt gaggcacctc cggcccctaa cagcaccatc gttttgcctt tgatatcaaa
     1261 accgctctct ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc cacgcagata
     1321 gccatcatca ttaacgatgg tgttgatggc acccaccagt ttggctgctg gcgttaattc
     1381 atcaacatat tcacacgcca gttgtttgtt cggcatcgac acaccagttc cgcgcatttt
     1441 gagggctttt aatccttcaa ttgctccggg aaagctatcg ttatccactt cgaaggccat
     1501 ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcag gcgataaact
     1561 gtggcggata ggataagcca tcaatccaat caattcgtat tttgcggtaa catccataat
     1621 taactccttt aacctctatg cttaattgtg tttaaccggg tgctaaattt gcgttcgcca
     1681 aaccgcacat cattctcagg aataataaaa acgcggtaat agcggctaaa aacaataatt
     1741 gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata ttgcaggccg
     1801 atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc acccatcatc
     1861 atataaatac tggtgacttt ggctttgctt ttggggaaaa actctgacat gaccgaaaca
     1921 ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggcgcc ggcattacac
     1981 accagtggag aagggtacag gtagataatg gcagctgtta ttgttgccag cgcagagtta
     2041 aatacattag cccagatggg ccggaccatt tttttcagta gtgcggcaaa aataaagaca
     2101 cagaccaacg agcccatact gtaataagag atggttttta atgcctcagc ttctgacata
     2161 ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa ggtcgagaat
     2221 gcggctacac cgaacagtac cgatgaaaca ccttccagcc agactaacgg tttgctgttc
     2281 atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa tttgcttttc
     2341 aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc atagccgtac
     2401 cagatattat tgagcaacat atagctcacc agcattgggt agaacatttg cccaaatgac
     2461 accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca ttccatgagc
     2521 gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac tgccagaccg
     2581 taggcgagag ttaaattcgg gcaagcggga ataccaaaga agaatagcag atacattatt
     2641 actgccatta atatcaccgc ccgacgacca aacttatcgg agatcacacc gaagaataaa
     2701 atactgatca atcgccccaa accgataccg gaaattaaat aggcaatgcc cgcgttgtca
     2761 gtggaaaact tttctgccag agatgtcata ttttgggcaa gcgtaataac actaatgccg
     2821 tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt gctgaaagat
     2881 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     2941 ttttccatgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3001 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3061 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3121 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3181 tatcgaacca cataatatcg gcaatacttc tttgggacag atgagccgta atcaacggaa
     3241 tagtaaaggt cgcaatactg cccgcactgt aatagatccc tgtagcttta cctttagcgt
     3301 aagggaaacg ttcagctatt aacgtcaggc caatttgcac aacacctcca gcagaggtaa
     3361 aaccaatgac aaaagcaaat attatcacca cataaaatgt gggatgcagg cagacggtaa
     3421 acagagcaat aaatgagata aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat
     3481 tacgaatgag tggagcggta ataaatacac aaagcagcga acccacggtg tagatactga
     3541 gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc
     3601 actggctaac cagataaaac gttgccattg agatatagcc atataaggta taactggcta
     3661 aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg acaggtaagc
     3721 gacgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta ataaacataa
     3781 tgcctgcagc aatcataaag gaccaaccga accacagttc agcccacact aacaggctaa
     3841 tgattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa atattggctg
     3901 tcccaggtga gcgtggaaaa gcttccatca aactgggata agtgcctgca tcgagaaaac
     3961 tgtttgccat tcccgccaga aagccaaaaa cataagcgat aatgatgtta ttggtctgca
     4021 ggatgccaaa aaagaaggcc atatagcagc acattccgag catgataaaa gggcggcgac
     4081 caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca atgcccagcg
     4141 atgagataac tatcgagaca cccgcggcat tagtctgaca aagtgtctcc agcgaggcca
     4201 tattcaggct catcaaaatg acgcccatac catgcaccag gtaataaaat acaacccaag
     4261 tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct ttaaaaagtg
     4321 tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt cctttctgtg
     4381 gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta ccgattgata
     4441 gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg
     4501 gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga tggcatgaag
     4561 atgttttttt cgttactgac gcatagccaa cagttgctcg actacacaat caggaatggc
     4621 gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg caatccaggt
     4681 cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt cataagcgtt
     4741 catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat cagcctgctg
     4801 acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg aggggcgagg
     4861 tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa agactacaca
     4921 cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat gttgatttat
     4981 aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg tctcattagt
     5041 ttaaaaactg taaagaattt gcgacaggta ctaccagctg aatggcggta agcaggaaaa
     5101 tatggggaag agcaagcaaa tatcaattag cggtgattaa caatcaggcg gcagagccgc
     5161 ctgatgagaa attacttatt cggtttctcg atttcgccaa gttcttcgag aatttcttcc
     5221 ggttggtccg tcggcggcgg gacttcttcc acccacgcgg caaacagacg ccaggaaacg
     5281 gctaacagaa ccggaccaat aaacagaccg atcatcccga aagcaatcaa accaccaata
     5341 acgccagaga ggatcaggat cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg
     5401 atgacgttat ccagcgtgcc aaccacaccg ctccacacta acaataccgt tccccaggtg
     5461 gtatcgccgg tccagtagag ccagataatc gccggaatca gtaccggcaa cgggccaagc
     5521 tggacaaggc aggagaggat cattaacacc gttagcagcg ttgcataagg tacgccggat
     5581 acggcgaggc cgataccgcc aagcactgcc tgtactaacg ccgtcaccac cacacccagc
     5641 gccaccgcga ggatagcctg tgccgccagc agcacggcgg catctccgcg aacgcctgcc
     5701 agacgggttg caaaatggcg aatgccttgt gccacctgtt caccgcgcca gtacagcagg
     5761 gcactgaaga gaagcatcag cgcacaatgc accataaagc gcccgatatg cgccgcctgc
     5821 ccaacgaacc aagtggtggt ggtgccaata taagggcgga ctttcgccat gatcgccgta
     5881 ccccccatat ccagcaagtt gtgccagcct gcatacagct tcgcgccaat caccggaatg
     5941 gtattaagcc acgccagatc gggtaacgtc atgtcgccgc tggaaatggc tttaattagc
     6001 gggccgctgc cgtcgacgat actgttaacc agcaaagcaa tagggatgat aaacaccatc
     6061 actaataaca gcgtcatcac cagaacggcg agagagcggc ggccaaacat gatcttttgc
     6121 aaacgtaaca ataccggcca ggtggcgata accaccgtac cggcccatgc aaagccgaga
     6181 ataaagggtt gaacaatcca cagacatgcc acaatcatga tggctaaaaa cagcaccgaa
     6241 agcagaattt gtgcgacatc cctgggctga cgaacattta ccataactac ttttcacctt
     6301 ttttgtgcgt caaatcgtcg acgcgacgtg gacacgcgaa actcacctgc ataatgatga
     6361 gcaatttcag cggtttttaa caggccgatt ctgtccttaa ttctgatggg cgtgttagaa
     6421 aaaaatgtgg tacaacaact caggcaacac gcaaacgatt tactcgtcgt atttcaagtt
     6481 gcatgtgctg cggctacgtt cgttcacccc agtcacttac tattgtaagc tcctggagtt
     6541 cactcgcttg ccgccttcct gcaactcgaa atccatagag taaataaaca tcacctttca
     6601 gtgcgctatt gcagccgcct gaaagaatag tgtatcgacc tacatcacag accgcaggaa
     6661 agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca actggtgctt
     6721 aattttttgc aagagctgga gcaacaaggt tttaccggcg atgcggcgac aagttatgcc
     6781 gatcgtctga caatgtcgac cgacaacagt atttaccaac ttctccccga tgcggtggta
     6841 tttccgcgtt caaccgcaga tgtggtgctg atcgcccgtc ttgccgcgca ggaacgctat
     6901 tcatcgctga tctttacccc ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac
     6961 caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat taaccctgaa
     7021 gagggctggg tgcgcgttga ggccggggtg ataaaagacc aactcaatca gtacctgaaa
     7081 ccgttcggct acttttttgc gccggaactt tcgaccagca accgggcaac gctcggtggg
     7141 atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac gtcagatcac
     7201 gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca acctttaccc
     7261 gtcgagctgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat ttataacacg
     7321 gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc caaacttaac
     7381 cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac cgagttcgac
     7441 ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga agcgcggctg
     7501 gatattacgc gcttgcctaa agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac
     7561 tctgcgctgc gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt agagacggtg
     7621 gactcaaaag tgctgaatct ggcgcgggaa gatattgtct ggcattccgt cagcgagttg
     7681 attaccgatg tgcctgacaa agagatgctc ggactgaaca ttgtggaatt tgctggtgat
     7741 gatgaggcgc tgattgacga gcgagttaat gcactctgtg tgcggcttga tgagctgatt
     7801 gctagccaac aggcaggtgt gattggctgg caggtgtgcc gcgagctggc gggggttgaa
     7861 cgtatctatg cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct
     7921 aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacatctggc cgattatatt
     7981 gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt cggtcacgtc
     8041 gacgcaggtg tcttgcacgt ccgtccggca ctggatatgt gcgatccgca acaagagatt
     8101 ttgatgaagc aaatctctga tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg
     8161 tggggcgagc acggcaaagg ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa
     8221 ctttttgcag aactgcgcaa agtgaaagcg gcatttgacc cgcataaccg actcaaccca
     8281 gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag
     8341 cgcggtacat tcgatcggca gatccccatt gcggtacgcc agcagtggcg cggtgcgatg
     8401 gagtgtaacg gcaacggttt atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg
     8461 atgaagatca cccagaaccg gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa
     8521 tggctgcgct tgttggcgga tcgcggcgtt gacccactca aactggaaca agaactgcct
     8581 gaatccggcg tcagtttgcg gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat
     8641 aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg tctggcctgt
     8701 aaagcttgtt cgacccagtg ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt
     8761 ctgcagctct atcacacccg ttatttacgc ccgcttcgcg accacctcgt tgctacggtc
     8821 gagagctacg caccgctgat ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag
     8881 ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg
     8941 gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac gctggaacag
     9001 cttgaagctc tcaatgcaga gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt
     9061 accagctatt acgatgcgca agtggtggcg gattttgtcc gtctggttga aaaattaggt
     9121 ttccagcctg tgttactgcc attttcgcca aatggcaaag cccagcatat caaaggcttt
     9181 cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg tatggctaag
     9241 cttgggatgc caatggtggg cgtcgatccg gcgctggtac tttgttatcg cgatgaatat
     9301 aaactggcgc tgggcgagga acgtggcgag tttaacgtct tacttgcgaa tgaatggctg
     9361 gcaagcgcac ttgagtcaca tccagtggct acagtcagcg gtgaatcatg gtatttcttt
     9421 ggtcactgta ccgaggttac cgctttgccg ggcgcgccag cacaatgggc cgcgatattt
     9481 gcccgttttg gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact
     9541 tacggacatg aagcgaaaaa ccatgaaaat tcgctcggga tctatgagtt atcctggcat
     9601 caggctatgc agcgactgcc gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc
     9661 caggtaaaac gggttgaagg cacgggggta cgccatcctg tgcaggcttt actggagatt
     9721 attaaatgat atggaaacgg aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa
     9781 acatggtggg gttcctggat attcgctttg aacatattgg tgatgacacc cttgaagcga
     9841 caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga ggagcatccg
     9901 tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa
     9961 aagtggttgg tctggaaatc aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc
    10021 gcggcatatg taaaccgttg catctcggtt cgcgtcacca ggtctggcag attgaaatct
    10081 tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt
    10141 gtcaccagaa aagtgtgacg catattgctt ttggtcatta tctgcacagt aaacggtggt
    10201 atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt gatcccaccc
    10261 agctggccat tgaattttta cgccgtgacc agagcaatct ttcacctgcc caatatctca
    10321 aacgtctgaa acaactagaa cttgaatttg ctgacctgct tactctctct tctgctgaat
    10381 taaaagaaga aatctacttt gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg
    10441 caagttcgcc aaatcgacct ggattaacct ccgtccaggt tcgatgacct tcttaatact
    10501 gcttatctac cgcctgatat tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg
    10561 cgggttagct ttatgagatt attcttatcg ccccttcaag agctaagcca ctgagagtgc
    10621 cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag acttaagggt
    10681 tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat tttcaataca
    10741 actttttctt tttcatcaca gccttaatgc tccttagata ataaccatta tctaacaatg
    10801 agatacctaa ttcttagcgt gcctgttaac ccacacatca ggggctatgc ttattaaaag
    10861 caggcaaaaa gcatgtttta cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca
    10921 ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaata gatggacatg
    10981 cattcaggaa cctttaaccc acaggatttc gcctggcaag gcttaacgct gacacccgca
    11041 gcggcgatac acatccgcga gctggtggca aagcagccag gtatggtcgg cgtgcgctta
    11101 ggtgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg
    11161 gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg
    11221 atgccgttta ttgatggcac ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc
    11281 aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag
    11341 gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg accggcggcc
    11401 cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg gcaaagggga
    11461 taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg
    11521 agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac tggttgaaag
    11581 cgcactacga caagctgaat tatcaggatt acagctacta ctcagcacca tcgtgcggta
    11641 attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct
    11701 ttttaagtaa agaggtggag gcggcgtttg agcagttggg cgttcccgtg cgggaaggca
    11761 aagaggtggc ggtggatgcc attttcgact ctgtttcggt tgccaccact tatcgcgaaa
    11821 aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac gatcacccgg
    11881 aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac
    11941 ttaatgcggc ggtagcctct gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc
    12001 cgatggaact ttccacctat tttcgcatta acgcggaaaa aaccgggcag tttgagcgca
    12061 caattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt tccgctccgg
    12121 tgcgtgacag ctatcagttg cacgcggcag tggtggaagt catcatccat aaaaacgccg
    12181 aggtgaaata ttccacggta caaaactggt tccctggcga taacaacacc ggcggtattc
    12241 tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc
    12301 aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg cgcggcgata
    12361 actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg
    12421 gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg aaagggatct
    12481 ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg acggcaacca
    12541 atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt ggggcgcata
    12601 ccttcccgta tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag gcaacgacat
    12661 cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc gaagaagacg
    12721 ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcagaactg ccgctggaat
    12781 ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc ggataaggaa
    12841 taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct atcctgcgtg
    12901 gattaagcct cgacgttcgt cccggcgaag ttcacgccat tatggggcca aacggttcgg
    12961 gcaaaagtac tttatcggca acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca
    13021 cggttgagtt caaaggcaaa gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag
    13081 gcatctttat ggccttccag tatccggtgg agattccagg cgtcagtaac cagtttttcc
    13141 tgcaaacggc acttaatgca gtgcgcagct atcgcggcca ggaaacgcac gaccgctttg
    13201 attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa gatttattaa
    13261 cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa
    13321 tggcggtgct ggaaccggag ttatgcattc ttgatgagtc ggactccggg ctggatattg
    13381 acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag cgctcattca
    13441 tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac gttcatgtgc
    13501 tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc
    13561 agggttatgg ctggcttacc gaacagcagt aatgcgctgc aacagtggca tcacttgttt
    13621 gaagctgaag ggacaaaacg ctcaccgcaa gcacagcagc atttacaaca attgctgcgt
    13681 accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga agggctgacc
    13741 aatagtcagt ttgtcagcat tgcgggagat atatccccac agcagcgtga tgccttagcg
    13801 ttaacgttag acgccgtgcg gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc
    13861 gatgcaattg aaggcagcgg atatgaagtg agcattaacg acgaccgtca ggggttaccc
    13921 gacgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg
    13981 catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat gcatatcacc
    14041 cagggcgtgg caggtgaaga ggtgaacact gcccattacc ggcatcatct ggatctggcg
    14101 gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt
    14161 accggggcac ggttcactat caatgtcgca gcgaacgccc acttgcagca tatcaagctg
    14221 gcgtttgaaa acccggtcag tcaccacttt gcccataacg atttgttgct ggctgacgat
    14281 gccaccgcat ttagccatag tttcctgctg ggtggcgcag tgttacgaca caacaccagt
    14341 acgcaactca atggcgaaaa cagcacgctg cggatcaata gcctggcgat gccggtgaaa
    14401 aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg taacagccga
    14461 cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac
    14521 gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaataatct gctgatgggc
    14581 aaactggcgg aagtggatac aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc
    14641 agccacggcg cgacggtggg gcgtattgat gatgaacaga tgttctatct gcgctcgcgc
    14701 gggatcaatc agcaggatgc ccagcagatg atcatttacg cctttgctgc cgaactgacg
    14761 gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg tcaacggctg
    14821 ccaggaggtg caagatgact ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt
    14881 cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt gcacagaaac
    14941 cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg gcggtgcatc
    15001 gcggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg
    15061 catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg
    15121 aagggatcaa tctggtcgcc aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca
    15181 tcatcatcag tcagatggag caccacgcta acattgtccc ctggcagatg ctttgcgcac
    15241 gcgttggcgc agagctgcgt gtgatccctc tcaaccccga cggtacgctg caactggaga
    15301 cgctgcctac gctgtttgat gagaaaactc gcctgctggc aattactcat gtctccaacg
    15361 tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa
    15421 aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggatgtt caggcgcagg
    15481 gttgcgactt ttacgtgttc tccgggcata aactgtatgg ccccaccgga attggcattc
    15541 tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc ggttctatga
    15601 tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg cggtttgaag
    15661 ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg
    15721 cgctggggct taatagcata gccgagtatg aacagaatct gatgcattat gcgctatcac
    15781 agctggaatc tgtaccggat ctcactctct atggccaaca aaacaggctt ggcgttattg
    15841 cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat aattacggca
    15901 ttgctgtgcg taccggacat cactgcgcta tgccattaat ggcctattac aacgtccctg
    15961 cgatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat cgtctggtga
    16021 ccggcctgca acgtattcac cgtctgctgg gataacaggg aggcactatg gctttattgc
    16081 cggataaaga aaaattgctg cgtaattttt tacgctgcgc caactgggaa gagaaatatc
    16141 tctacattat tgagctgggc cagcgtctgc cagaattacg cgccgaagac ggaagcccac
    16201 aaaatagcat tcagggctgc cagagtcagg tgtggattgt catgcgccag aatgcgcagg
    16261 gaattattga attgcagggc gacagcgatg cggcgattgt gaaagggctt attgcggtcg
    16321 tctttattct ctacgatcag atgacgccgc aggatattgt caatttcgat gtgcgtccgt
    16381 ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa ggtctggaag
    16441 cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa gggacagctt
    16501 tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc acaggagtgt
    16561 tttggtttca ggtgaacata agcaccatcc gacgtttcag cgtgagtctc cggcaaatac
    16621 aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg cgcttttagc
    16681 gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag ccgactggtt
    16741 gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc tattgcccga
    16801 cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc cccggtgcca
    16861 aaacctggta cgacgataac tattccttca caactgttat tacctgatgc accgcgtcag
    16921 gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg agaaaatatt
    16981 gtgcaggttt atccaatagg tattggattg caggggctgg aaacgccggt gatggaaacg
    17041 cgtgttgggc agaaaatccc taacccaacc tggacgccta cggcaggcat tcgtcagcgt
    17101 tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa taacccgcta
    17161 ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca tggcaccagt
    17221 gcgccggaca gcgttggttt gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat
    17281 attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg
    17341 gtgaaatatt ccgtggaacc taacgggatg cgttatgttg aagtacatcg accactatca
    17401 gcagaagaac agcaaaacgt tcagacaatg ccatacacac tgccagcagg ctttacgcaa
    17461 tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta tcgtcgggca
    17521 gggtatccgg tttcggtgag cagtggagca actcccgcag ccagcaatgc gccttcagta
    17581 gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgcgac gcagtagtgg
    17641 taaatagcag acaaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc acaggtacta
    17701 ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc
    17761 tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt
    17821 agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc tggagcaacc
    17881 tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag ctttcattat
    17941 taataccctc tagattgagt taatctccat gtagcgttac aagtattaca caaagttttt
    18001 tatgttgaga atattttttt gatgggaagg cacttatttt tgatcgttcg ctcaaagaag
    18061 catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt ccatcggatt
    18121 catcttaaat aaagtgagat tatatagtga aatccgattt g
//
LOCUS       DAJTRQ010000076        17571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000076 DAJTRQ010000000
VERSION     DAJTRQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(266..1294)
                     /gene="epmB"
                     /locus_tag="OO332_002628"
     CDS             complement(266..1294)
                     /gene="epmB"
                     /locus_tag="OO332_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5943056.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1336..1902
                     /gene="efp"
                     /locus_tag="OO332_002629"
     CDS             1336..1902
                     /gene="efp"
                     /locus_tag="OO332_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5943057.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1954..2079
                     /gene="ecnA"
                     /locus_tag="OO332_002630"
     CDS             1954..2079
                     /gene="ecnA"
                     /locus_tag="OO332_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5943058.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2190..2336
                     /gene="ecnB"
                     /locus_tag="OO332_002631"
     CDS             2190..2336
                     /gene="ecnB"
                     /locus_tag="OO332_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5943059.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2512..2741
                     /locus_tag="OO332_002632"
                     /pseudo
     CDS             2512..2741
                     /locus_tag="OO332_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2738..3271)
                     /gene="blc"
                     /locus_tag="OO332_002633"
     CDS             complement(2738..3271)
                     /gene="blc"
                     /locus_tag="OO332_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5943060.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3360..4493)
                     /locus_tag="OO332_002634"
     CDS             complement(3360..4493)
                     /locus_tag="OO332_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5943061.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4556..4915)
                     /gene="frdD"
                     /locus_tag="OO332_002635"
     CDS             complement(4556..4915)
                     /gene="frdD"
                     /locus_tag="OO332_002635"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5943062.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4926..5321)
                     /gene="frdC"
                     /locus_tag="OO332_002636"
     CDS             complement(4926..5321)
                     /gene="frdC"
                     /locus_tag="OO332_002636"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5943063.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5332..6066)
                     /gene="frdB"
                     /locus_tag="OO332_002637"
     CDS             complement(5332..6066)
                     /gene="frdB"
                     /locus_tag="OO332_002637"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5943064.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6059..7867)
                     /gene="frdA"
                     /locus_tag="OO332_002638"
     CDS             complement(6059..7867)
                     /gene="frdA"
                     /locus_tag="OO332_002638"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5943065.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8192..9169
                     /gene="epmA"
                     /locus_tag="OO332_002639"
     CDS             8192..9169
                     /gene="epmA"
                     /locus_tag="OO332_002639"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5943066.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9388..10890
                     /gene="yjeM"
                     /locus_tag="OO332_002640"
     CDS             9388..10890
                     /gene="yjeM"
                     /locus_tag="OO332_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR5943067.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11041..14352)
                     /gene="mscM"
                     /locus_tag="OO332_002641"
     CDS             complement(11041..14352)
                     /gene="mscM"
                     /locus_tag="OO332_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5943068.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14374..15342)
                     /gene="psd"
                     /locus_tag="OO332_002642"
     CDS             complement(14374..15342)
                     /gene="psd"
                     /locus_tag="OO332_002642"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5943069.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15439..16491)
                     /gene="rsgA"
                     /locus_tag="OO332_002643"
     CDS             complement(15439..16491)
                     /gene="rsgA"
                     /locus_tag="OO332_002643"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5943070.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            16586..17131
                     /gene="orn"
                     /locus_tag="OO332_002644"
     CDS             16586..17131
                     /gene="orn"
                     /locus_tag="OO332_002644"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5943071.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17342..17417
                     /locus_tag="OO332_002645"
     tRNA            17342..17417
                     /locus_tag="OO332_002645"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17375..17377,aa:Gly,seq:gcc)
BASE COUNT         4071 a         4520 c         4611 g         4369 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg ccgataagcg tagcgcatca ggcaatttta
      241 atgtttaact tccctgttta atcagttact gctggcgtag ctggagatcc agcggcgttt
      301 tgctgggttc gccgccaatt tctcgcgcca gtttcggcac cagatatccc gacaccaatg
      361 tcagcaactc acgcataatc tgccgtgctt cgtcatcact caccataaaa tgcgccgcgc
      421 cctgtacttt atctagcaca tgcaggtaat acggcatcac gccggcatcg aacaacgcat
      481 tactcaggtt tgccaacgtt tgtgcgttat cgttcacacc acgtaacaga acgctctggt
      541 taagcagggt gacaccgaca cggcgcaatt tagccatcgc ctgacggaac gtttcatcta
      601 cctcattggc atgattgatg tgattcacca gcaagatttg cagcgtagaa cgggcaaagc
      661 gttcaaccag cgcctcggtg atacgtgccg ggatcacaat cggcagacgg ctgtgaatcc
      721 gcagacgttt gatatgcggg atggcttcca gttgtgtgag caaccagtcc agctcgtgat
      781 ctttcgccat cagcggatcg ccgccggaga aaatcatctc gtccagttcc ggatgcgcag
      841 caacatactc aagtgcagtc tgccagttac gcttgttgcc ctgattttcg gcatagggga
      901 agtggcgacg gaagcaatag cggcaattta ccgcgcagcc gcctttgacc agcaaaagcg
      961 cccggttgtg gtatttatgc aacaaaccag gcactacgct gtgctgttct tccagtgggt
     1021 cggtggagaa tccggacgcg acgacaaact catcttgcga ggtaagtacc tgacgcaaaa
     1081 gaggatcgtc cggattgcct ttctccatgc gatcgataaa tgagcggggc acacgcaggg
     1141 caaacagctt tttggcgctg cgtccggcca acagtttttc gtccgcgtct atattcaaaa
     1201 gacgcagaag ttcatcagga tcggtcacaa catcggcaag ttgcgttaac caatcttctc
     1261 tggatggggt atttagggtt acaatatgcg ccattttgtg gcttagctac caattaacaa
     1321 atttcagagg gccttatggc aacgtactat agcaacgatt ttcgtgctgg tcttaaaatc
     1381 atgttagacg gcgaacctta cgcggttgaa gcgagtgaat tcgtaaaacc gggtaaaggc
     1441 caggcatttg ctcgcgttaa actgcgtcgt ctgctgaccg gtactcgcgt agaaaaaacc
     1501 ttcaaatcta ctgattccgc tgaaggcgct gatgttgtcg atatgaacct gacttacctg
     1561 tacaacgacg gtgagttctg gcacttcatg aacaacgaaa ctttcgagca gctgtctgct
     1621 gatgcaaaag caattggcga caacgctaaa tggctgctgg atcaggcaga gtgtatcgta
     1681 actctgtgga atggtcagcc gatctccgtt actccgccga acttcgttga actggaaatc
     1741 gttgataccg atccgggcct gaaaggtgat accgcaggta ctggcggcaa accggctacc
     1801 ctgtctactg gcgctgtggt taaagttccg ctgttcgtac aaatcggcga agtcatcaaa
     1861 gtggataccc gctctggtga atacgtctct cgcgtgaagt aatgcggttg tggtgcggcc
     1921 tgcaggctgc accatcactt attcaggtca gagatgatga aacgccttat cgttcttgtt
     1981 ttgcttgcca gcacgctgct cacgggctgt aacaccgctt gcggtttcgg cgaagacatc
     2041 aaacatctcg gcaactccat ctctcgcgct gccagctaat ttttcttctc ttccgaaaaa
     2101 tcatcagatt tccatcattt ttggcgatgt tgtctattat taatttgcta taggcaaaca
     2161 taaataacat tacctaaaag gaagacgtta tggtgaagaa gacaattgca gcgatctttt
     2221 ctgttctggt gctttcaaca gtattaactg cctgcaacac cacgcgtggc gttggtgaag
     2281 acatttctga tggcggtaac gcgatttctg gcgcagcaac gaaagcgcag caataagcaa
     2341 taacggtacg acagcagtgt cgtgccgttt gttttttctg cgatcgtcat aaaggtaata
     2401 gttgaaattc ccctgccacc tggcaaaata tccgttcaac catcagcttt gcaggacgac
     2461 ctgcaaacgc ctcttttcac cggggacggc cccaaatttc cggagcctga tatgtcctgg
     2521 attatcttag ttattgctgg tctgctggaa gtggtatggg ccgttggcct gaaatatacc
     2581 cacggcttta gccgtttgat gccgtgtgga cgggtattgg cgcagtcggc gcggccatca
     2641 ccggcattgt gctgctcggt gagtccgcta acccgatgcg tctggcgagt ctggcgttaa
     2701 tcgtattggg gattattggt ctgaaactca gcactcacta actaccgggc tgctgtaccc
     2761 aaataaactt actgacatca aacccttccc gggtcgcgac tgccagcatc tcctgtttca
     2821 cttcgtcaga aatggttggc gtgcgggaga gtatccacag gtagtcgcgg tccgggccgc
     2881 aaaccagcgc atggcggtat tcccgatcga gtgcaataac gttataaccg ccatagaaag
     2941 gaccaaagaa tgacactttc agcgcagcgc gagttggtgc gccggtaaag tacgctttcc
     3001 cttcactctg ctgccacatt cctctgtcag ggttatagcc tttattaatg acattcaggc
     3061 cgccgtcatc acgcaggctg tatgttgcgg tgactttttc cagtccacgt tcaaaacggt
     3121 gatcaaaacg ggcaatctca taccaggtac cgagataacg tttggcgtcg aaattattca
     3181 ctacggtcac gccacgcggc ggcgtaggag aactacaggc aacgaccaga aatgcagctg
     3241 tcgctgcggc aacgagaggg agcaggcgca taaatgtttc cttactggtt tttttctaag
     3301 tgtagatgac agcaaggaaa agcgcagaaa aggtccgaaa attcggaccc gatgaaattt
     3361 tactgtagag cgttgagaat ctgacaggcg gcggcgactc tctctggatt gggatagttt
     3421 ttgttagcca gcatcacgat acccagctct ttttctggaa taaacgcgac atagctacca
     3481 aatccgccgg tcgcccccgt tttatgtacc catgatgcgc gtactgcagg agttgggggc
     3541 ttaatcgctt ttaccgggcg tgctgccagt gcaattttat tgtcactgcc gttaatgatg
     3601 ctgtcaggat tgaccggcca gtccagcatt tcccagccca ggccctgata catatcgccg
     3661 gtttgccagt agcgagattg tgccagttgt atcccttgtt gaagcgtttt ctcattgata
     3721 tcaagggggg gtaaattgct ttgcacccag cgggccatat cttcaatggt cgacttcaca
     3781 ccataagctt cagcatctaa cgcccctggc gaaacatgca ctgccttacc ttcgcgatat
     3841 ccccaggcgt aattcttttc ttctggggga ggtacattaa tccacgtatg gttgagtttg
     3901 agtggctgga agacacgagt ttgcatcgcc tgctcaaaac tcaaaccaga cggcttcaca
     3961 gccagtgcgc cgaacaaacc gatactggag ttggcataca gacgttgtgt tcctggggcc
     4021 catgcaggct gccagttttg atagaagcgc agcaagtcgc ttgaggattt cacttcatcc
     4081 ggcacctgca atggcaggcc gccagcggtg taggtcgcaa gatgtaatag tgtgatccca
     4141 ttccactgtt tagcggtaag ttcaggccag tattttgttg cgggatcgct taacttgatt
     4201 tcccctcgag caatagcgtc gccaccaagc acgcccgtaa atgttttgct gaccgaacct
     4261 aactcaaaca acgtttgctg tgtgacgggc tgctttttgg cgatgtccgc atagccccaa
     4321 gtaaagtaat aaggtttacc ctgataaatt accgccaccg ccataccggg gatcttttgt
     4381 tgctctataa gcggggtaat tgtgcgatgc acaatatcgt tgatttgttg aggggcagca
     4441 aatgtggagc aagaggcggt aattaataag gcgcagagcg tcgttttgaa cataaggtct
     4501 ggtttccata caaaacggcc cgccataggc gggccggatt tacattggcg atgcgttaga
     4561 ttgtaacgac accaatcagc gtgacaactg tcaggatagc agccagaccg tagaaaaccc
     4621 atttgcccgc aggcacgtgg attttcagat cgtgcatcgc gtggtgcata cggtgtaaac
     4681 cacaccacag cggcagaacg atcatcagga acaggaatac gcgaccaatg aagctctgcg
     4741 cgaacgccag aacgcgctcg tagctcagcg catcacccgg aaacagcccc agtggcagca
     4801 gaatacccac cagcaggatc atcaccggcg caataattgc gctccacata ccaccggccc
     4861 cgaagaggcc ccagaatacc ggttcgtcag aacgctttgg atttggatta atcatctcag
     4921 gctccttacc agtacagggc aacaaacagg attacgatgg tggcaaccac agttaccgcc
     4981 cagagacttt tgataattgg ctctggtccc attttttcgt cttttacaat gatattggcc
     5041 gctttcggtg ccagttcaaa ccaggttttg gtgtgcagca gagctgccgt cagagtgatc
     5101 aggttaatga tcacgataac cgggttttgt aaaaagtcga cgaatcccgc ccaggcttcc
     5161 gggccatttt tcagggcaaa cagcccgaaa atcagttcaa tgctgaacca cacagccgga
     5221 accgccgtgc cttcgcgcag catgtaaaag cgataaaacg gcaatttttt ccaccaggtg
     5281 gacgtcattg gccgtacata cggtttacgt ttagtcgtca tgttgcactc cttagcgtgg
     5341 tttcagggtc gcgataagaa agtctttcga actttctact ttgccctgct gaatggccgc
     5401 agccggatcg acgtgtttcg ggcagacttc ggagcaatag cccacgaaag tacagctcca
     5461 tacaccgttc tggctgttca actgcgccat acgctccttc ttaccgtggt cgcggctatc
     5521 ttcgttataa cgatgcgcca gcgtaatggc agccggaccg atgaactctg ggttcaggcc
     5581 aaactgcggg cacgcggcgt agcacagacc acagttgatg caaccggaga actggtgata
     5641 ctttgccatc tgcgccgggg tctggatgtt agtaccctga tccgcggtgc gggagttgcc
     5701 gatgatgtac ggtttgatcg cttccagact ttcgatgaag tgggtcatat cgaccaccag
     5761 atcgcgttca atcgggaagt tagctaacgc ttcaaccttc ataccgtcgg tgtaatcacg
     5821 caggaaggtt ttacatgcca gttttggcac gttgttaacc atcatgccgc aggaaccaca
     5881 aatcgccata cggcaggacc agcggtaact caggtccggt gccaggttgt ctttgatgta
     5941 gcccagcgca tccagtaatg aggtagttgc gtcataaggc acttcataga atgcgctatg
     6001 cggtgcggta tcgacttccg ggttatagcg caccacctca attttcaggt ttttcatctc
     6061 agccattcgc cttctcctta ttattggctg cttccgcctt atcggctgca tccgcttcgc
     6121 caccgtaaac gcgtttagct ggcggcagcg tagtaatctt cacgtcgctg tactccaggc
     6181 gagtcgtgcc atcagcatcg cggaaggcga gggtgtgttt gagaaagttg acgtcgtcac
     6241 gctcggtgca accttcgtcc agacgctggt gtgcgccgcg ggactcttta cgtgccattg
     6301 cggagtgcgc catacattca gcaacgttca gaccgtggct cagttcaatg gtgtagagca
     6361 ggtcggtgtt gaacacgctg gaggtgtcgg tgatgcgcac gcgcttgaag cgttcctgca
     6421 gctctgccag cttgtcgatg gttttctgca tcagttccgg cgtacggtag ataccgcaac
     6481 cttcttccat tgccaggccc atttcgtcgc ggatcttcgc ccagttttcg ccgccatcct
     6541 ggttaaccag atctttcaga cgttgttcaa cgccagctgc ctgcgcttca attgccgctt
     6601 cgttgccatt accggcagtt gctgcacgct ctgtcgcttg ttcaccggcc agacggccga
     6661 agaccaccag ttccgccagg gagttggagc ccagacggtt tgcaccgtgc agaccaacag
     6721 aggaacattc acccacggcg aacagacctt taatgcgggt ttcacagttc tgatcggttt
     6781 cgataccgcc catggtgtag tgtgcagtcg gacgtaccgg aatcggttct ttaaccggat
     6841 cgacgccaac gtacgctttc gccagttcgc agatgaacgg cagacgttca tgcagttttt
     6901 tctcgccgag gtgacgcagg tcgagataaa ccacatcacc acgcggcgtg gagatggtgt
     6961 tgcctttacg ccattcgtgc cagaaggcct gagaaacttt gtcgcgtgga cccagttcca
     7021 tatatttgtt tttcggctcg cccagcggag tttccgggcc catgccgtaa tcttgcagat
     7081 aacggtagcc atttttgttg accagaatac cgccttcacc acggcagcct tcggtcatca
     7141 ggataccgga acctggcaga ccggttgggt gatactgaac gaattccatg tcacgcagcg
     7201 gaacgccgtg gcttagcgcc atacccatac cgtcaccggt aacgatgccg ccgttggtgt
     7261 tgtaacgata aacgcgaccc gcaccgccgg tagccataac gaccgcgtta gcacggatct
     7321 gcaccaacgt gccttccatc atgttcattg ctaccaggcc gcgaacatga ccatcatcaa
     7381 ccagaatatc cagcacgaaa tgttcgtcaa aacgctggat ctgcgggaat tgcagagagg
     7441 tctggaacag cgtgtgcagc atatggaagc cggtcttatc ggcggcgaac caggtgcgct
     7501 cgattttcat gccgccgaag cgacgtacgt tgacgctacc atccgggcga cggctccatg
     7561 ggcatcccca cagttccagt tgggtcattt cggttgggca gtggtggacg aaataatcca
     7621 cgacatcctg ctcacacaac cagtcgccac ccgctactgt atcgtgaaag tgatattcga
     7681 agctgtcatg atcctgcgcg acagcggcgg agcccccttc tgcagcaacg gtatggctac
     7741 gcatcgggta tacttttgag attagtgcga tttttgcatt tggatttgcc tgcgcggcag
     7801 caattgcagc acgtaatccc gcgccaccgg cgcctacaat ggcaagatcg gcttgaaagg
     7861 tttgcacgac attcctccag attgttttta tcccacagcc ccgtacttca gggtaagtgc
     7921 ctgaaagtta cggtctgcga acgctattcc actgctcctt tataggtaca acagtatagt
     7981 ctgatggata agtctgaaat ttgacgagat cgattttttt agtgcgcaag agggctaaat
     8041 tatcactgaa gatgattaat ttaattacta aaccatcaga tcgtgctttt tttagtcact
     8101 gcccaccgct gtttgattcc tgcgtaaaat ttgtctcgcc gtcgcgttgc gagtagactt
     8161 cgtgcccttg tcaaaaactg gagatttaac tatgagcgaa acggcatcct ggcagccgag
     8221 cgcatccatt cctaacttat taaaacgcgc ggcgattatg gcggagatcc gtcgtttctt
     8281 tgccgatcgt ggagtgctgg aggtggagac gccttgtatg agccaggcga cggtaaccga
     8341 tattcatttg gtcccgtttg agacacgttt cgttggcccc gggcattcgc aggggatgaa
     8401 tctctggtta atgaccagcc cggaatacca tatgaaacgc ctgctggttg ctggttgtgg
     8461 gccggtattc cagctgtgcc gcagcttccg taatgaagag atggggcgtt atcacaaccc
     8521 tgagttcact atgctggagt ggtatcgacc gcactatgat atgtaccggt tgatgaacga
     8581 ggtggacgat ctcttacaac aggtgctgga ctgcccggca gcagaaagcc tttcttatca
     8641 acaagctttc ttgcgttatc tggaaattga cccgctctct gccgacaaaa cgcaactgcg
     8701 ggaagtcgca gcgaaactgg atttgagcaa tgttgctgat accgaagaag accgcgacac
     8761 gttgctacaa ttgctgttta cctttggcgt agagccaaat attggtaaag aaaaaccgac
     8821 ctttgtgtac cactttccag ccagccaggc atcactggcg caaatcagta ccgaagatca
     8881 tcgggtcgct gaacgctttg aggtttatta taaaggtatt gagctggcga atggtttcca
     8941 tgaattaacg gatgcccgtg agcagcaaca acgctttgaa caagataacc gtaagcgcgc
     9001 ggcgcgcggt ttgccgcagc accccattga ccagaatctg attgaagcct tgaaagtcgg
     9061 tatgcctgac tgctccggcg tggcattagg tgttgatcgt ctggtgatgt tggcgctggg
     9121 cgcggagaca ctggctgaag tcatcgcctt tagcgttgac cgggcataat tctgaaaaat
     9181 tactgaatta acagcgaaga atggcgtgat aagcgccatt ctgtagcata tgttttcatt
     9241 tttccgttaa aggccacaat ttaagactgg atatttgata tcatcaaggt atcaattctg
     9301 tattggtttt tacgctgcct gctcaatgtt gcgcagtgca gttcagtggg cacgtgttat
     9361 acacgcgctg aaatgaagga tggtttcatg cctcacacga taaaaaagat gagtctgata
     9421 ggactcatat tgatgatctt tacttccgta tttggatttg ccaatagccc atcggcttat
     9481 tacttaatgg gttatagtgc gattcccttt tatatatttt ctgcattgtt attctttatt
     9541 ccattcgcct taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggtggtatc
     9601 tattcctgga tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg
     9661 ttttcctctt atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca
     9721 acattcctct atggtagtga catgacccag cactggcgta ttgccggact ggagcctacg
     9781 caggtggttg gtctgctggc agtagcatgg atgattctgg tcaccgtcgt tgcttcaaag
     9841 gggattaata aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat
     9901 ttagtattgc tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag
     9961 gatattaatt tccttgcatc accgaacccg ggttatcagt ccggtctggc aatgctatcg
    10021 tttgtggtat ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat
    10081 aaaacggaaa atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt
    10141 tcaatcggtt attcgctggc aatattttta tggggcgtca gcacaaactg gcagcaggta
    10201 ttaagtaatg gttccgttaa ccttggcaat attacctatg tgctgatgaa gagcctcggg
    10261 gtgacgctgg gtaacgcact gcatttgtca cctgaagcgt cattgtcgct gggcgtatgg
    10321 tttgcgcgta ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt ctttacgctt
    10381 tgctattcac cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg
    10441 atgacgcgcc tgaatgcgat ggggatgccg tctatcgcca tgtggatgca gtgcgggttg
    10501 gttactgtct tcattctgct ggtttcgttt ggtggcggta ccgcatcggc gttctttaac
    10561 aagctgacgc tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct
    10621 ttcccgtttt ttaaagcacg tcaggatctc gacagaccat ttgtgatttt caaaacgcgt
    10681 atgtcggcaa tgattgcgac ggtggttgtc gttctggtgg tgacatttgc gaacgtcttc
    10741 accattattc agcctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc
    10801 ggccctgtct tcttctcgct gttagcgatg gcgatttacc agaactattg cagtcgcatg
    10861 gcgaataaac ctgagttagc tctcgactga tcatatgaaa tcctgcaact catcgtctct
    10921 ccggcgatga gttgtcagga agtaatgcgt atggatgata ctctccaggg ccggtgataa
    10981 ctgaagatga tgttaagctg ctaaatgagc cacgttgaca tcatttggca gcttcatgca
    11041 ttacaaactt cccgcctgac gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt
    11101 accgttgagg ctttccagac gcatctggaa gggcgggaat ggcatatcga taccgtgggc
    11161 gtggaagcct gccagaatca gctggtggat ctcatggcgt agcggcatac ggtgacccat
    11221 ctcagcggcg taaatacgca gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa
    11281 gacttccggt gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat
    11341 ttccgtcact tcttcactgt tggcatcggc aggggccgga atcgtcaaca ctacgcgcgt
    11401 gaccgagtca gagagcgacc agttgataaa ctgctcggta ataaacgcct tgttcggcac
    11461 gattatctct ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa tttttgtcac
    11521 gctaccagta agatcgcgga tcgttaccgt atcgccaatg cgaatcggtt tttcgaacag
    11581 gatgatcagg ccagagataa agttggcgaa aatttcctgc aaaccaaaac cgagaccaac
    11641 accgagcgcg gcaaccagcc actgcaattt cgaccactca ataccaatca ttgagaagcc
    11701 gaccagcccg ccaatcagca tcagcagata ttttgtgatg gtggtgatgg cgtaacccgt
    11761 acccggcgtt aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg
    11821 caccagctgc gtggtgatga taaataccag aatggcaatc agcaccgcac cgagggtaat
    11881 tggctccaga ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg aaatattttc
    11941 gaggaagccg aaagcggaat ggatttctga ccacagcaca atgaccgaaa gcagggcgat
    12001 caacatcaaa attgagcgca ccagccgcaa ggattgcgca ctgatggcat cgagatcgac
    12061 ttcgctttca tcgacttcaa ttgctccttc cgggctactg tgatgatgcg cttcctcttc
    12121 gccacgcgca cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt caaacgccag
    12181 cctgcgacgc tggatcagca tccagcggcg gataacgtga taaaccacca gtagcaggaa
    12241 ccagatggca atcgaggttt caagcctcgc taacagtgcc tgcgccgttg ccagataacc
    12301 caccgccgac gccagaatgg caaccagtgg tgcgccaatc atcatgttcc acagcatatg
    12361 gttggtaatg ttgtcgccgc tgccttcttt gttgagatac aacggaatac cggctttttt
    12421 caggcttagg gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag
    12481 cgaaccggaa aattcgcggt cgtcgaggtt atcgaacatc atcagcgcca taatcagccc
    12541 gatgctcatc aggtagtagc gcatcccacg ggaaacacgt tcgcgcggcc agccaaaatg
    12601 agcgataaac aagccgttcg ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag
    12661 cggcacggtg gccgttacac catcgccaat cgcgaccgcc agcggataag gccacgcctc
    12721 gcgcaagccg taacccagcg tcatccacag caccggtaac ggtgacgcga cgagaatcga
    12781 ccagaaaaga gtgcgcaacg tcagccagaa gtgatcctga gtcactttgc cgactttcgc
    12841 cgccgaacgt tcaagaaaac gggtgaaata gcggcgcgag taaatactgc aaccgaccag
    12901 aatcaacgcg ccaaacagcg gcaaaattgt ctctttgctg gtcagcatca tcacactggc
    12961 tttgcccaac tgactgaagg tgtccagcga aatgagacga cgcagatcct gggcgatttc
    13021 cagcggccag gcgatggtca tcgggcgcac gtcagaggtc cagaacagat agcggtgcgt
    13081 tgcttcgttc acctctttca gcgcatcctc cagttgcccg ttggagactt tcagcttggt
    13141 cagttccaat agtagcgtgt cgccaccctg caacaatgag ttcagcaact cacgctgagt
    13201 gcgcagttgt gcttccagaa tacggttttg ctcggcggtc agcggctgac cgtcggcctg
    13261 atggatttgc cgtagcagcg gctgtttatt aagtaaatcc tcataacgta accgttgcac
    13321 acgcaactgc gccatttcgg tatcaagctg ttgtggtttt ggcatttctg gtagccgtgc
    13381 cacctgcgcc cgcagcgctt cgccgagcag gttggacgat cccagccatt gcgactgttc
    13441 acgcagcgta ttcaacgcct gccggacctg taacgtctgg ctggcagcct gacgctgttg
    13501 cgaggcaacg agctccatcc gctgcgcctg ttgattcagc gctgccgata gttcgcggtt
    13561 aattttgaat tgcgcgacga tatctttcgg caaatcggca ctgttttctg ccagtaattc
    13621 ggtactttcc aacgcccgct ccgcctcaag ctgacgttgg ctattcaact gattacgtaa
    13681 gccctgcaaa tacgcatcca gttgctggct ctctttttcc gccagctctg agcgtaagcg
    13741 cgctaattcc tggcggttat tggcagacag ctgcgccagc tccagttcat caacgagcgc
    13801 cttaagacgt gcagagtcag actgcaacgc gaaattttgt gcctgattga gcggagtatt
    13861 gccggtaagc gttcccaggc ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg
    13921 ctgttgcggc agttgattca gcgaatcggc aatctcgcgg gcgcgctcct gctcttgctg
    13981 ggcctgacgg cttttatcca gcaactggct gctgacctgg agaatttcct gattcagcgc
    14041 gtcggtagac attcccggcg acacgctgcg cggctcgtca cgcatgttgt ttaattgtgc
    14101 gcgcagagta gcggagagtt tcggataatt atcgataact tgctgatatt gtttgatgcg
    14161 ctcaagggaa ccttttcgtt cctcaagcgc atttaaggca gactggagcg cctctacgac
    14221 ttccggctgt gcgggtttcg ccgcttttgc ctgctccagt tcctgagtga tttgtttgct
    14281 atcgggggcc gtcgcggcgt acgcccccca actgaggcac caggccatca gaaaagtgat
    14341 aatcaggcgc acgtcagcgt ttcctttgat ggattagacc tggtcttttt tatcgtcaac
    14401 caatggcctg gcgtcgtgtt ctgcttcgat ctcttcagca ggaagtgggg cgggttcagc
    14461 gtctggcgta acaaaggttt cggtagatac tgccagcggc tggccaattt tcgtgacaga
    14521 caggctttcc agttgctcaa ccagattcac tttacccggt gcaaacaggt tgataacggt
    14581 ggaaccgagt ttaaagcgac ccatttcctg gcctttcagc agtgccacag aaccgtcgtt
    14641 ttccccggca ggccaggtcc agcgcttgat gataccttcg cgcggcggcg taatggtgcc
    14701 cgcccagacc gtctcaatgc tgccaacaat cgtcgctccg accagaatct gcgccattgg
    14761 gccaaattcg gtatcgaaaa ggcaaatcac gcgttcgtta cgggcaaaca gattcggcac
    14821 gttctgagcg gtgagatggt taacggaaaa gagatcgccc ggcacgtaga tcatctcacg
    14881 cagaataccg ttgcacggca tgtgtacgcg atggtagtca cgcggggaga ggtaagtggt
    14941 cacaaacgta ccgttgcgga acaggtccgc catcagatag ttgcctgcca gcagggcttc
    15001 gaggctgtag ttgtggcctt tggcttgcag gattttatct tcttcgattt tacccaactg
    15061 gctgataacg ccatcggcag gcatgaccag tacattcgga tcggtatcga ttgggcgtac
    15121 ttcgtcacgc agcggacgga caaagaattc gttaaaggtg cggtagctgg cggtgtccgg
    15181 cttttgcgcc tctttcatgt cgaccttgta gtatttaacg aacagatcga taaccagttt
    15241 tgtcagccat cctgcccgct tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt
    15301 cggcagaatg tactgtagcg aaagtttaaa tgaatttaac aaggtagcct ccaggccatt
    15361 gttttgtcgt tcctgatccg gccaacatgc cggatcctga aaaaaagggg acgattctaa
    15421 cgacggttag cttaattgtc agccatccgt atcagaaaag tttttacgcg tttttacctg
    15481 cgccatgctt tccagaatac ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc
    15541 ctcaaccgct tcccggatag cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt
    15601 acacagacct aaatagtcat ggaattcgac aaagccctga gtgatttgtt ccggctccag
    15661 gtgccagagg ccgaactcac gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa
    15721 gtgatacagc cgagcggcgg tcgtggtgtg ctgaccgagc cccgagttgt cagagacatc
    15781 gttggtcagg atctcttttt gcagccccag caacgcgttc agcaggctgg atttaccgac
    15841 gccagactgc ccggcaaaaa tgctgatgcg cccggtcaac gcctcttcca gcggtttcag
    15901 cccatcctga gtatggctgg aaaccatcaa tacgcgataa ccgatgttgc ggtagatatc
    15961 catctgctcg ttgacgaacg ccatgccttc gtcgtccagc aggtctatct tgttgagcac
    16021 gataatcggc tcaatctgca aggtttcgca ggccaccagg taacggtcga taatattgag
    16081 cgacagctcc ggcaaaatgg cggagacaat gacaatctgg tcgatgttgg cggctatggg
    16141 tttcacgccg tcgtagaaat ccggacgcgt taacaccgag gtgcgctcat gcaccgcttc
    16201 cacgatccct ttgatattta cgccttccgc cgccggttta cccggacgcc agactacgcg
    16261 gtcgccggtt accagcgaac ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc
    16321 ggcggattcc acatcagcgt gcataccaaa gcggctgatg acgatacctt catcaggctc
    16381 gccaaacaga ttgtcgtcgt agtcgggctt ctccttagac gttttaagac gacgctggtg
    16441 attggcgttc acgcggcgct gctggccttt ggagagttta tttttactca atcgtactga
    16501 ctcctggtcg cccctgatgg gcaaaacatc tatgatacac gcaattgtgg atcaatatag
    16561 tcactgtgaa tgggtggaaa atagcatgag tgccaatgaa aacaacctga tttggatcga
    16621 tcttgagatg accggtctgg atcccgagcg cgatcgcatt attgagattg ccacgctggt
    16681 gaccgatgcc aatctgaata ttctggcaga agggccgacc attgcagtac atcagtccga
    16741 tgaacagctg gcgctgatgg atgactggaa cgtgcgcacc cataccgcca gcgggctggt
    16801 agagcgcgtg aaagcgagca cgatgggcga tcgggaagct gaactggcaa cgctcgaatt
    16861 cttaaaacag tgggtgcctg cgggaaaatc gccgatttgc ggtaacagca tcggacagga
    16921 ccgtcgtttc ctgtttaaat acatgccgga gctggaagtc tacttccact accgttatct
    16981 cgatgtcagc accctgaaag agctggcgcg ccgctggaag ccggaaattc tggatggttt
    17041 taccaagcag gggacgcatc aggcgatgga tgatatccgt gaatcggtgg cggagctggc
    17101 ttactaccgc gagcatttta tcaagctgta aaatttttcc tggtcacgta agcgccggaa
    17161 tccacttgcg gcggcgtgat tttgacgcta aatcgaacgt cttgctgata atttcagcac
    17221 ttgagataaa aacgcaaaaa aaactttttt ggggggttgc agagggaaag atttctcgta
    17281 taatgcgcct cccgtaacga cgcagaaatg cgaaaattac gaaagcaaaa ttaagtagta
    17341 tgcgggaata gctcagttgg tagagcacga ccttgccaag gtcggggtcg cgagttcgag
    17401 tctcgtttcc cgctccaaaa tttgaaaagt gctgcaaagc acagaccacc caatcgcagt
    17461 atttcgaatt gcgacaaggc ggcaactgaa cgaatccttg ggaacttaca aaagtatgtg
    17521 actaaggtga gtgaaggcag ccaaggcagt agcggttcga aagacgaaga t
//
LOCUS       DAJTRQ010000077        17448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000077 DAJTRQ010000000
VERSION     DAJTRQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            564..1334
                     /locus_tag="OO332_002646"
     CDS             564..1334
                     /locus_tag="OO332_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5943072.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO332_002647"
     CDS             1325..1798
                     /locus_tag="OO332_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5943073.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO332_002648"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO332_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5943074.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO332_002649"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO332_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5943075.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO332_002650"
     CDS             3233..3727
                     /locus_tag="OO332_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5943076.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO332_002651"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO332_002651"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5943077.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO332_002652"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO332_002652"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5943078.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="OO332_002653"
     CDS             7571..8485
                     /locus_tag="OO332_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5943079.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="OO332_002654"
     CDS             8699..10060
                     /locus_tag="OO332_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943080.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO332_002655"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO332_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5943081.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO332_002656"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO332_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5943082.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="OO332_002657"
     CDS             12610..13899
                     /locus_tag="OO332_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5943083.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="OO332_002658"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="OO332_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5943084.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="OO332_002659"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="OO332_002659"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5943085.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="OO332_002660"
     CDS             16225..16605
                     /locus_tag="OO332_002660"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5943086.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17448
                     /gene="hpaH"
                     /locus_tag="OO332_002661"
     CDS             16673..>17448
                     /gene="hpaH"
                     /locus_tag="OO332_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5943087.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGN"
BASE COUNT         4293 a         4624 c         4371 g         4160 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcat
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcat ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacat
//
LOCUS       DAJTRQ010000078        17393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000078 DAJTRQ010000000
VERSION     DAJTRQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO332_002662"
     CDS             complement(770..1966)
                     /gene="hemY"
                     /locus_tag="OO332_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5943088.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1969..3168)
                     /gene="hemX"
                     /locus_tag="OO332_002663"
     CDS             complement(1969..3168)
                     /gene="hemX"
                     /locus_tag="OO332_002663"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5943089.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(3190..3930)
                     /gene="hemD"
                     /locus_tag="OO332_002664"
     CDS             complement(3190..3930)
                     /gene="hemD"
                     /locus_tag="OO332_002664"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5943090.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3927..4868)
                     /gene="hemC"
                     /locus_tag="OO332_002665"
     CDS             complement(3927..4868)
                     /gene="hemC"
                     /locus_tag="OO332_002665"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5943091.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5255..7801
                     /gene="cyaA"
                     /locus_tag="OO332_002666"
     CDS             5255..7801
                     /gene="cyaA"
                     /locus_tag="OO332_002666"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5943092.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7841..8161)
                     /gene="cyaY"
                     /locus_tag="OO332_002667"
     CDS             complement(7841..8161)
                     /gene="cyaY"
                     /locus_tag="OO332_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5943093.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8209..9522)
                     /locus_tag="OO332_002668"
     CDS             complement(8209..9522)
                     /locus_tag="OO332_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5943094.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9519..10997)
                     /locus_tag="OO332_002669"
                     /pseudo
     CDS             complement(9519..10997)
                     /locus_tag="OO332_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11186..11389
                     /locus_tag="OO332_002670"
     CDS             11186..11389
                     /locus_tag="OO332_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5943095.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11426..12250
                     /gene="dapF"
                     /locus_tag="OO332_002671"
     CDS             11426..12250
                     /gene="dapF"
                     /locus_tag="OO332_002671"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5943096.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12247..12954
                     /locus_tag="OO332_002672"
     CDS             12247..12954
                     /locus_tag="OO332_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5943097.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12951..13847
                     /gene="xerC"
                     /locus_tag="OO332_002673"
     CDS             12951..13847
                     /gene="xerC"
                     /locus_tag="OO332_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5943098.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13847..14563
                     /gene="yigB"
                     /locus_tag="OO332_002674"
     CDS             13847..14563
                     /gene="yigB"
                     /locus_tag="OO332_002674"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5943099.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14647..16809
                     /gene="uvrD"
                     /locus_tag="OO332_002675"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14647..16809
                     /gene="uvrD"
                     /locus_tag="OO332_002675"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5943100.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16956..17348)
                     /locus_tag="OO332_002676"
     CDS             complement(16956..17348)
                     /locus_tag="OO332_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5943101.1"
                     /translation="MRLGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGAMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4058 a         4481 c         4601 g         4253 t
ORIGIN      
        1 cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg acagcaacaa
       61 caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca tgtcatcaaa
      121 atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga tgattacata
      181 aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga aaatcaggag
      241 agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat tatatcgcgt
      301 tgattattga tgctgttttt agttttaacg gcaattaata tatgtgttat taattgaatg
      361 aattttatca ttcataataa gtatgtgtag gatcaagctc aggttaaata ttcactcagg
      421 aagttattac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
      481 tgttatttcc cgattctctg tggcataata aacgagtaga tgctcattcc atctcttatg
      541 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      601 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      661 ccacttgttg tcatacagac ctgttttaac gcctgctccg taataagagc aggcgttttt
      721 ttatgcatca gaaaggtccc ggaggtgctt tcctccgggt gagaaggaac tactgtggcg
      781 ggttattctg caacgttaac atcaaaccgt cgcgacgcat cgctgcagct tcttccggct
      841 tgtgcagtct gtccagcgcg tcggcaagcc atgcgtaatc gtaggcgtcc ggacgttgtt
      901 tcagcgccgc gcggaaggcg agcgatgctt cctgccattc accgtgcttc atcagtgact
      961 gacccaatgt gctccacaac agcgggcgat caccgacgtt tttgatttgc tggcgtagca
     1021 ctttttccag ctgttccggg ttattggttt tcagacgcgg gatcggcagc agtaggcgat
     1081 cgtcatactg gcgtttcagg ccgtcgatga taatttgctg ggcagtatca tgatcgtcac
     1141 attcaataag atgttccgcc attgccacct gcaacgctac ctgatgacgc gttttccggc
     1201 tttggttttt ccaccagtta cgcaaacctt cactaccgtt atcggcacgc gcctgatcca
     1261 tcaggccaat ccatgcctgt tgttccagca ttgcacgatg ttcttcatca ccaacatggg
     1321 ctttcgccat tgatgggatg atatccagca gagaactcca tgcccctgtg cggatatacg
     1381 cctgttccgc cagacgtaat acttcaggat ggcgtggcgt aacttccagc agcttatcta
     1441 cgccgtggcg tgcagcatgg ttttcattac gggccagttg cagacgtaca cgggtgattt
     1501 ctaccggaat ggtgtcattg ccggccagct ccgctgcgcg ttccagatgt tggttggcgc
     1561 gtgcttcatc accacgttgt tgcgcggctt cggcagccag tagatagttc accaccggtt
     1621 gttcagcgtg atcggcattt ttcgccatca gcttttcaac ttgctgataa tcaccttccg
     1681 ccagtttcag cagcgcctgt tcggtctgct tacgcgcacg gcgacgctta cgtccgacaa
     1741 accatccacg ggtgtgcgcg ccagtgcgga agatccgccg cagtagccac tcaatggcaa
     1801 acagcactac catcgccaga atcaatatga tcgccaggcc cgtgacgctg gtttcgatat
     1861 tgtagttgtc ggtctggatc agcacataac cctgatgacc ggcaatcatc gggccaacca
     1921 ctatccccgc aatcagcaaa acaaagagta ataacacttt tagcatggtt attctccttg
     1981 cggcgcggct gccggagtat caccttgcgg tgcaggtgca ggtgcaggtg caggtgcagg
     2041 tttagcttcc gttgtccccg ctgccggttg tgccagcagg ttacgcacgc gagtctgcat
     2101 cagtttttcc agcatcgcct ggctttgcag ggtttccgga agatccatcg agatattttg
     2161 ctggcttaac tggtccacct cgtcgaggaa cgctttggtg gtggcatcat cagtatcgta
     2221 gtaagcacgt acccaggtgg agacgttctc cagcgcctgg cgataagtct cttcctgatg
     2281 acgcggtaca gcttgtgctg cgaccagcag gcgagagcga atattttcgc gcagatagat
     2341 atcctgattt ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa tcgtaatgaa
     2401 gttgtccata aagttctgcc agcttttttg cagattgata cgccattcgc tgatggaact
     2461 ggaaagctct tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat cggccagacg
     2521 caggttatct acctgatttg aaagctgatt aagcttaagg atgatgccgt cataatccac
     2581 ctgcgatact gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa cggtaatcag
     2641 actcgggtca ttcatatccg ccaggctggc gtctgcactt ttcagcaacg ctgcagaggt
     2701 cgtgacgtcc tgatcgctcc acagcttccg tccggcgagt ttcaccagaa aatcggcctg
     2761 agccagcagc caggttttag catcgctgcc ggaaatggtg gcgatctttt gttggacttc
     2821 atccaactgt tttgccagcg tttcttgctg acgattcgcc tgcttaagct gcgcagcttg
     2881 ttgcttaata atgccttcca gctcggcttt ttggctctcc tgggcttttt gcaatgccgt
     2941 cagttggtta gccagggcat cgctggtggc ggtctgattg acggcctgtt gtttacccca
     3001 gccatacaaa ccgacacccg ccgccagagc aatagcgata gccaccgcgc tgagaatcaa
     3061 tgcagtattg ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg tgtccacggc
     3121 ctccctggtc tcttcaacca cggcggaggt tttttcttgt tccgtcatta tggcttcctg
     3181 ttatgagagt tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat cggcgacctt
     3241 aatgtcttgc cagcccagtt cccgggcgag tttcgccaaa cgctcactga cgaccaatag
     3301 tcgacagtgt agtaaccagt gctcacgata ccattgtggg atcagcgacc agagttgctg
     3361 caacatttca ccgctggtaa caacgaccat cgtcacctcg cgggattgcc agcgcatcgc
     3421 ttcttctgca ccatcgtaat ggattgcgca tcgttgataa cattcacaaa aagtgacctc
     3481 agcaccgcgc accgtcaggg tatccccaat tagctcacga ccgccattgc cacgtaatat
     3541 cagcgcacgt ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga cttcgctgat
     3601 ttcccgatcc tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg ccagtgcggt
     3661 ggtgcgtcca atggtgaaat aagtaggtag ttggggccat ttacgatctt gctgatgcag
     3721 ctgtgattgg gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat cgctctcccc
     3781 cagcgctgcc agttgatcag caagttgcgg taattgtcga cccggagaaa actcaatcag
     3841 cggaaaatgc caggccacct gccccagtgt gcgcagacgt ctcactaact cttctccagc
     3901 gggagacggg cgggtgacca ggatactcat gccggggcct ctccgttata gacttcagcg
     3961 aggatctcgc gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc catttgttcg
     4021 gcatcttgcg gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc cggcgcgccg
     4081 accagcgcac atagccagat ttcgccatca ataagctcgg cgtagctacc aattggcacc
     4141 tgacatccgc cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg cagtgcagtt
     4201 tcgtggtgat tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag gcggcattca
     4261 ataccgactg caccttgtcc taccgccgga agagaaatct cgggtggtaa cgcggcgcga
     4321 atgcgggact ccagacctaa acgttttagt ccggctacgg caagaatgat ggcatcgtat
     4381 tcgccgttat ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga gcggataatc
     4441 agatccgggc ggcgttcagc cagttggcac tggcgacgta aactggacgt cccgacgata
     4501 ctgcctgctg gtaacgcatc cagactgtca tagttattgg acacaaaggc atcgcgagga
     4561 tcttcacgct cgcaaatagt gaccagcccc agaccttgcg ggaattcaac cggcacatct
     4621 ttcattgaat gtacggcgat atcagcgcga ttctccagga gcgcgacttc cagctctttt
     4681 acaaataagc cttttccgcc tactttcgcc agcggcgtat caagaatcac atcgccgcgc
     4741 gtcaccatcg gtaccagttc aacgaccagg cccggatggc tcgccatcaa cttgtctttg
     4801 acatagtgtg cctgccagag tgcaagtggg ctttggcgtg tggcaattct taaaacattg
     4861 tctaacatgc ttgttaccgt cattatcatc cgtggtccat cctaacatcc ttgccagagt
     4921 gatgtcagtg ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga ggtctggcag
     4981 tggatcctga caggcgtttc acgccgtcgt aataaggaat ttacagagaa taaacggtgc
     5041 tacacttgta tgtagcgcat ctttctttac ggtcaatcag caaggtgtta aattgatcac
     5101 gttttagacc attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag tggtaacggt
     5161 tacctttgac atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt ggcggaatca
     5221 cagtcatgac gggtagcaaa tcaggcgata cgtcttgtac ctctatattg agactctgaa
     5281 acagagactg gatgccataa atcaattgcg tgtggatcgc gcgcttgctg ctatggggcc
     5341 tgcattccaa caggtctaca gtctactgcc gacattgttg cactatcacc atccgctaat
     5401 gccgggttac cttgatggta acgttcccaa aggcatttgc ctttacacgc ctgatgaaac
     5461 tcaacgccac tacctgaacg agcttgaact gtatcgtgga atgtcagtac aggatccacc
     5521 gaaaggtgag cttccaatta ctggtgtata caccatgggc agcacctcgt ccgtagggca
     5581 aagttgttcc tctgacctgg atatctgggt ctgtcatcaa tcctggctcg atagcgaaga
     5641 gcgccagtta ctacaacgta aatgcagcct gctggaaagc tgggccgcct cgctgggtgt
     5701 ggaagtcagc ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa gcggcagcct
     5761 ggggggcgaa gattgtggct ccacccagca tatactgctg cttgacgaat tttatcgtac
     5821 tgccgtgcgt ctcgccggta agcgtattct gtggaatatg gtgccgtgcg acgaagaaga
     5881 gcattacgac gactatgtga tgacgcttta cgcgcagggc gtgctgacgc caaatgaatg
     5941 gctggatctc ggtggcttaa gctcgctttc tgctgaagag tactttggtg ccagcctttg
     6001 gcagctctac aagagtatcg attccccata caaagcggtg ctgaaaacac tgctgctgga
     6061 agcctattcc tgggaatacc cgaacccacg tctgctggcg aaagatatca aacagcgttt
     6121 gcacgacggc gagattgtat cgtttggcct cgatccatac tgcatgatgc tggagcgtgt
     6181 tactgaatac ctgacggcga ttgaagattt cacccgtctg gatttagtac gtcgctgctt
     6241 ctatttaaaa gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct ggcgtcgcgc
     6301 agtgttgagc cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg caatgctcga
     6361 taaccgtgct aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc tgctcgacgc
     6421 gatgatgcag agctaccgta atctgatccg ctttgcgcgt cgcaataacc ttagcgtctc
     6481 cgccagtccg caggatatcg gcgtactgac gcgtaaactg tatgccgcgt ttgaagcatt
     6541 gccaggtaaa gtgacgctgg taaacccgca gatttcaccc gatctctcgg aaccgaatct
     6601 gacctttatt tatgtgccgc cgggccgggc taaccgttca ggttggtatc tgtataaccg
     6661 cgcgccaaat attgagtcga tcatcagcca tcagccgctg gaatataacc gttacctgaa
     6721 taaactggtg gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc gtttgtatat
     6781 taaaggtaac ggcattgtcg atttgcctaa gttgcaggag atggtcgccg acgtgtcgca
     6841 ccatttcccg ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc cgtgtgagat
     6901 tcgccatctg gcgattatcg ttaacctgga atatgacccg acagctgcgt tccgcaatca
     6961 ggtggtgcat tttgatttcc gtaagctgga tgtcttcagc tttggcgaga atcaaaattg
     7021 cctggtaggt agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc gtacgctgca
     7081 cttcaacggc gagcaatcga tgatcgaagc cctgaaaact attctcggca aaatgcatca
     7141 ggacgccgca ccgccagata gcgtggaagt cttctgttat agccagcatc tgcgcggctt
     7201 aatccgtact cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc tttccagcac
     7261 ccgccaggaa accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct ggggcttgtt
     7321 cttcgaacgc ctgaatgtat cggtacagaa gctggagaac gccatcgagt tttatggcgc
     7381 tatttcgcat aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc acgtcaaatt
     7441 accggcggtg gtggacggct ttgccagcga agggatcatc cagttctttt tcgaagaaac
     7501 gcaagacgag aatggcttta atatctacat tctcgacgaa agcaaccggg ttgaggtgta
     7561 tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct tctactcgtc
     7621 atcgcacgac cgcttcacct acggctcaag cttcatcaac ttcaacctgc cgcagttcta
     7681 tcagattgtg aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa aatcgattgg
     7741 caacctgccg cccgccaatc aggatcacga tacgccgcta ttacagcagt atttttcgtg
     7801 atgagcgtgc cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa ctgactgttt
     7861 cacccgcctg ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg ccgctgcgat
     7921 cgcaaatcca ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg gttgccagcc
     7981 acacctggtg cagcggctcc tggcggttaa taatgatttt gctgccgttt tcaaaggtaa
     8041 tggtcagtac gccgccgttg atttcgcagt cgatatcgct gtcgccatcc ctgtcgtcca
     8101 gacgctcttc aatggtcagc cacagttgat cagccaggcg atgaaattca ctgtcgttca
     8161 ttgttgtacc ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc agggcttcaa
     8221 agtttgcacc tctgctgctg cgttccggca tgattcatcc gtcaccggaa taatgatgtc
     8281 tctgttttga acttcacgta cgttctcctc tctgacacga gttttcgccg ggcaggggga
     8341 gggcgtaatc cttacctctg tatcggtcca tgtataaaca ttgcgatcgt cataagcgaa
     8401 gcgatcgttt ttagttagcg gaccgttaac tggaataatt tgcaagctct ttgaccgtaa
     8461 ttggcggata aagtaagcat gctgtttgcc gctattaaat gagcgatcaa tgaccaagtc
     8521 tggcgtatcc agcgggatta tctcaagctg gtcttcacca aatgtcgata attttgttac
     8581 atacagccga tctttatatt gcgcaatatt gccagtaatc agatggaatt gttcaggttc
     8641 aatacctggt aggtctatcc acttgctcca ctgacggtcg ggcgtgagct gtttacgcca
     8701 gcgaacgcga ctttccccta cttcaaaaga gacgtcatca tctacgggta tcgctttgcc
     8761 tgataattga ccaaagggat aacgatgcag acccttattg tcgcgaaaaa ccagcagtga
     8821 atgaggtatc cagcgaatta tctcaaatga gtctgcattc gccgtaaggt gaacgccatt
     8881 taaataaata ttctgcccac tgcggataag cagatcattg gaatatgaat ttctgtttac
     8941 actcataaga cgtggattaa taccccacca tcttttttct aatacagtaa catcactgct
     9001 gctcccctga cgtcgaccgg acaagtagag atttttacta tccaccagcg tggtagagtt
     9061 accttcgata gcttttagtg tcgccagatc aacacgtgac gcgccgctat tactttcggt
     9121 gcgctgtccg tcaaaataga ggctatattt atcgaccgcg aagcggccca tagcctgaaa
     9181 gctggcaata tcgacagtgg gtgttcccgg tggattgcta acgattttgc cacgccagag
     9241 aataacgcgg ccgtctgtca cccagtggta ttcctcattt tctatgctta tttcatcgga
     9301 aggtatataa cccgttttcc cagagatatt tttcacaagg cgcaaaccgg ccctgctcat
     9361 ctccggaaac tgggcaatga ttttcttatc cccccaataa cctttaccgt aagacataac
     9421 accgtgatca ttgtaatagt cgaaccctat ggcctgtaca gcaggcgtca gaaatgtgtg
     9481 tcccaacagg atgagtgcaa ctgtgattat ttgacccttc attttgtcac ctcattgcgc
     9541 ggggcaggaa taaattcgcc gtcatcagta atttttccgt ccagatatcc cgttttgtaa
     9601 tttacgccat agacatatcc cgaatgttgt tccttccaga ttaagcgaac ttgtttcgga
     9661 tcagcggtgc ggtatcgcca gagctgcgcg ccatcaaagc tgtaaatgta gcggttatcg
     9721 cgggcatacc atctgcgcaa aaagggatgg tcattaagat gggcttcgcg ttcatccgga
     9781 gaacggttat ccagtaagat cagcggtccg tcagattcaa acacgtgtaa accggaatgg
     9841 tatggctacc cttcggcacg cgtaagtaaa tagccgtggc gtttcccggc gttgaaacgt
     9901 ttgttgataa taagattggg atcatcaagt ttcacgatct ccagcttacg gtcaccagac
     9961 tctgtttcga tgatggtata aagcgagtcc tgatactggg cgacagcatc actgatgggt
    10021 tgaaactgct ccggatcgag tccgggtaat tcttcctgtt tacaagcagg tcctttacgc
    10081 cagaacactt tcttttccag cagagaaaaa tccagacagt gctttgttaa atcttcatcc
    10141 cacgccagat tttccttgtc gaggacttta cgctgcagcc cgtttttatc acgccaggtc
    10201 aagagtgagc caggcatcca gcgcacgacg gaaaaggtgt ccgggtcggc atcaagctgt
    10261 tcgccgttga gcaggatttt ggtccgtgta cgagccaggg tatcccaggg gccaaatggt
    10321 acggcaatgc aggggttaaa cgtggtggaa aactttcctc gctgatccca ggacttctgc
    10381 gccagcacac ggaaactctc ggggtcagcg aggcgatgcc cattgatata caagaaatta
    10441 gcgtcacgta atatcagccc cagtaagtca ggatcccagg gaggccggaa ttccacctga
    10501 tgcagcgttt tgatatccaa gctatttcca ccaccattgt catcggtgcg cttgccttca
    10561 aaatagagac tgtgtttatc tgcggcaaaa tcaccccatg ccttgaacga ggcgacatcg
    10621 acgggaggtg tgccgggagg gttttgcaca acttcacccg cgtagaggat attgcgacca
    10681 tcgctatgcc atgaataatg aggatactag gcctgtgcgc cctcatagag gtagtcggtt
    10741 atcaaatcag tcagaggcgg ttctttatcc cattcgtcta gtgttgtcgg gtctattaat
    10801 aaattgggta accgacgcaa actcttgtaa ttcacgtcag gtagagaaag atttgtttct
    10861 ttataagggc gcaggtacac cacgcctttt ccctgtttct ggtaaatatt atgttcaggt
    10921 gcggaaagcc gacatcccca tgccgacgat aaaaacaatg aacaagccaa caaacaaacc
    10981 acattgcgat agtgcataaa gccatcctgg cgcgaggtgt tgatcacgaa actgccagca
    11041 gaacataaat cctcacgagt aagcgttata ctcgcagcat ttcctcactt ttcagacttc
    11101 ataaagagtc gctaaacgct tgcttttacg ccttctcctg cgatgataga aagcagaaag
    11161 cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca ctgtattact
    11221 tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc cgcctgcaga
    11281 taaaaacgca ccgccgccga ccaaaccggt agagacgcaa acgcaatcca cggtgccgga
    11341 taaaaacgat cgcgccactg gcgatggtcc atcccaggtg aattactaaa agtcagtttc
    11401 tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc ttggcaacga
    11461 ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc tgattcgtcg
    11521 cctggcggat cggcacctgg gggtagggtt tgaccagctg ctggtggttg agccgccgta
    11581 tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg aagtggcgca
    11641 gtgcggtaac ggtgcgcgct gctttgcccg ttttgtgcgt ctgaaaggac tgaccaataa
    11701 gcgtgatatc cgcgtcagca ccgccaacgg gcggatggtt ctgaccgtca ccgatgatga
    11761 tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tccgccgtgc cgtttcgcgc
    11821 taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct tatgcggcgt
    11881 ggtgtcgatg ggaaatccgc attgcgtgat tcaggtcgat gatgtcgata ccgcggcggt
    11941 agaaacgctt ggtcctgttc tggaaagtca cgagcgtttt ccggagcgcg ccaatatcgg
    12001 ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc gtggggcagg
    12061 agaaacccag gcctgcggca gcggcgcgtg tgcggcggtg gcggtaggga ttcagcaagg
    12121 tttgctggac gaagaagtac gcgtggaact ccccggcggt cgtcttgata tcgcctggaa
    12181 aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg acggatttat
    12241 tcatctatga agcaactagg ggaagaactg caggaaacac tcacggagct tgatgaccgg
    12301 gcggttgtcg attatctgat taaaaatcct gagtttttta tccgcaatgc gcgcgcagta
    12361 gaagcgatac gtgtgccgca tccggtacgc ggcaccgttt cgttggtcga gtggcacatg
    12421 gcccgcgcac gtaatcacat tcatgttctg gaagagaaca tggcgttgtt gatggagcag
    12481 gctatcgcca acgaagggct gttttatcgc ctactctacc tgcagcgcag tctcaccgcc
    12541 gccagcagtc tcgacgatat gctgatgcgc tttcaccgct gggcgcgcga tctcggcctg
    12601 gctggtgcga gtctgcgcct gtttccagat cgctggcgct taggtgcgcc gtcgaaccac
    12661 actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg tttggggcag
    12721 gaacagcact atcttgggcc gcttaacgga ccggagctgc tggtggtgct accggaagcg
    12781 aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt gggtgttgtg
    12841 ctgtttacca gtcgcgatgc cagtcactat caacaagggc agggaacgca gttacttcat
    12901 gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt atgaccgatt
    12961 tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcgtcag cttagcccga
    13021 taaccctact taactaccag cgtcagcttg aggcgatcat caattttgcc agcgaaaacg
    13081 gcctgcaaag ctggcaacaa tgcgatgcag cgatggtacg caattttgct gtacgcagtc
    13141 gccgtaaagg gctgggagca gcaagtctgg cgttacggct ttctgcgcta cgtagctttt
    13201 ttgactggct ggtcagccag aacgaactca aagctaaccc ggcgaaagga gtttcggcac
    13261 cgaaagcgcc gcgtcatctg ccgaaaaata tcgacgtcga cgatatgaat cggctgctgg
    13321 atattgatat caacgatccc ctcgctgtac gcgaccgtgc aatgctggaa gtgatgtacg
    13381 gcgcgggtct gcgtctttcc gagctggtgg ggctggatat caaacacctc gacctggagt
    13441 ccggcgaagt gtgggtgatg gggaaaggca gcaaagagcg ccgcctgccg attggtcgca
    13501 acgctgtggc gtggattgag cactggcttg atttgcgcga cctgtttggt agcgaagacg
    13561 acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg cagaaacgct
    13621 ttgccgaatg gggcataaaa caagggctga ataatcacgt tcacccacat aaattacgtc
    13681 actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg caggagctac
    13741 tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt caacaccttg
    13801 cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc gtttttaccg
    13861 gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt acgataaccg
    13921 tccggtgatt ttgcgcaccg agcgagaggc gcttaccttt gtgcaaaatt atcatccggc
    13981 gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac gggaagcgga
    14041 acccgagatt tatcacgacg tgacgcgttg gcgttttcgt tcgattgaac aagcgatgct
    14101 cgacgccggg ctgagtgccg aagaagccag tgcaggcgca cacgcagcaa tgattaactt
    14161 tgccaaatgg cgcagccgaa tcgacgtccc gcagcaaact cacgacacct taaaacagct
    14221 ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gcccagccgg aactgtttgg
    14281 tttgggggat tattttgagt ttgtgctgcg cgctggcccg cacgggcgct caaaaccgtt
    14341 cagcgatatg tactttttgg ctgcggaaaa actcaacgtg ccgatcggcg agatcttaca
    14401 tgttggggac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa tgcaggcttg
    14461 ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gacagccgtt tactgccaca
    14521 tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa atctgtatat
    14581 atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt ttacgcggcg
    14641 gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacaacg cgaagcggtg
    14701 gccgcgccac gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa gacgcgcgta
    14761 ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata ctcgattatg
    14821 gctgtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg acaactgatg
    14881 ggcaccagcc agggcggcat gtgggtaggc accttccacg ggctggcgca ccgcctgctg
    14941 cgtgcgcacc atatggacgc caatctgccg caggatttcc agatcctcga tagcgaagac
    15001 cagctacgcc tgcttaagcg tctgatcaaa gccatgaacc tcgacgagaa gcagtggccg
    15061 ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg tccgcatcat
    15121 attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca ggcgtatcag
    15181 gaagcgtgcg atcgcgcggg cctggtggac ttcgccgagc tgctgctgcg cgctcacgaa
    15241 ctgtggctta acaagccgca tatcctacaa cactaccgcg aacgttttac caatatcctg
    15301 gtggacgaat tccaggatac caacaacatt cagtacgcgt ggatccgcct gctggcgggc
    15361 gacaccggca aagtgatgat cgtcggtgat gacgaccagt caatctacgg ctggcgcggg
    15421 gcgcaggtgg agaatattca gcgtttcctt aatgatttcc ccggtgccga aattattcgt
    15481 ctggagcaaa actaccgctc taccagcaat attctgagcg ccgctaacgc cctgattgaa
    15541 aacaataacg ggcgtctggg taaaaaactg tggaccgatg gcgcggacgg tgagcctatt
    15601 tccctctatt gcgcttttaa cgaactcgat gaagcgcgtt ttgtggttaa ccgcatcaaa
    15661 acctggcagg acaacggcgg ggcgcttgcc gagtgcgcca ttctctaccg cagtaacgcc
    15721 cagtcgcgtg tgctggaaga agcactgttg caggcgagta tgccgtaccg tatttacggc
    15781 gggatgcgct tcttcgaacg ccaggaaatc aaagatgcgc tctcgtatct gcgcctgatt
    15841 gccaaccgta acgacgacgc ggcctttgag cgtgtggtga atacgccaac gcggggtatt
    15901 ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt aacactctgg
    15961 caggcatgta gtgaactgtt gcaggaaaaa gccctcgccg gacgtgctgc cagcgcctta
    16021 cagcggttta tggaactgat cgacgcctta gcgcaggaaa ctgccgatat gccgctgcat
    16081 gtacagactg accgggtaat taaagactcc ggcctgcgta ccatgtacga gcaggagaag
    16141 ggcgaaaaag gtcagacgcg tatcgaaaac ttagaggaac tggtgacggc aacgcgccag
    16201 ttcagctaca acgaagaaga cgaagattta atgccgctgc aggcgttcct atcccatgcg
    16261 gcgctggaag caggcgaagg gcaggcggat acctggcagg atgcggtaca actgatgacc
    16321 atgcactcgg cgaaaggtct ggagttcccg caggtgttta tcgtcggtat ggaagagggc
    16381 atgttcccaa gccagatgtc gctggatgaa ggcgggcgtc tggaagaaga acgccgtctg
    16441 gcctacgttg gcgtaacccg cgcgatgcag aaactgacgc tgacctacgc ggaaactcgc
    16501 cgtctgtatg gcaaagaggt ttaccatcgc ccgtcgcgct ttattggcga gttgccggaa
    16561 gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag ccatcagcgg
    16621 atgggtacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg tgtacgccac
    16681 gctaagtttg gtgaaggcac catcgtcaat atggaaggca gcggtgaaca tagccgtttg
    16741 caggtggcat tccagggcca gggaatcaaa tggctggtgg cagcttacgc ccggctggag
    16801 tcggtgtaac gttgccggat gcggtgctgc gcaccttatt tggcctaaaa aatcattcag
    16861 attcaataaa ttgcaacgtc atgtaggcag gataaggcgt ttacgccgca tccggcatca
    16921 gcagcatatt tagtatctga cacaaagcta tcattctaac ctttccgctc gacggaaatc
    16981 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
    17041 atcgtgccac caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
    17101 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
    17161 atcccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt
    17221 acaccgtttt ccatcaacat tgctcctgac tgcaccgcaa actgaatatc tttactggct
    17281 ttaaaagcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
    17341 agacggataa agaaattccc ttcacctgaa gcgagattta acgctacctt ctg
//
LOCUS       DAJTRQ010000079        17254 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000079 DAJTRQ010000000
VERSION     DAJTRQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..642)
                     /gene="rlmB"
                     /locus_tag="OO332_002677"
     CDS             complement(<1..642)
                     /gene="rlmB"
                     /locus_tag="OO332_002677"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5943102.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELI"
     gene            complement(822..3263)
                     /gene="rnr"
                     /locus_tag="OO332_002678"
     CDS             complement(822..3263)
                     /gene="rnr"
                     /locus_tag="OO332_002678"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5943103.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3302..3727)
                     /gene="nsrR"
                     /locus_tag="OO332_002679"
     CDS             complement(3302..3727)
                     /gene="nsrR"
                     /locus_tag="OO332_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5943104.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(3932..5230)
                     /gene="purA"
                     /locus_tag="OO332_002680"
     CDS             complement(3932..5230)
                     /gene="purA"
                     /locus_tag="OO332_002680"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5943105.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5334..5531)
                     /locus_tag="OO332_002681"
     CDS             complement(5334..5531)
                     /locus_tag="OO332_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5943106.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5613..6617)
                     /gene="hflC"
                     /locus_tag="OO332_002682"
     CDS             complement(5613..6617)
                     /gene="hflC"
                     /locus_tag="OO332_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5943107.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6620..7879)
                     /gene="hflK"
                     /locus_tag="OO332_002683"
     CDS             complement(6620..7879)
                     /gene="hflK"
                     /locus_tag="OO332_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5943108.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7965..9245)
                     /gene="hflX"
                     /locus_tag="OO332_002684"
     CDS             complement(7965..9245)
                     /gene="hflX"
                     /locus_tag="OO332_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5943109.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9321..9629)
                     /gene="hfq"
                     /locus_tag="OO332_002685"
     CDS             complement(9321..9629)
                     /gene="hfq"
                     /locus_tag="OO332_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5943110.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9715..10665)
                     /gene="miaA"
                     /locus_tag="OO332_002686"
     CDS             complement(9715..10665)
                     /gene="miaA"
                     /locus_tag="OO332_002686"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5943111.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10658..12505)
                     /gene="mutL"
                     /locus_tag="OO332_002687"
     CDS             complement(10658..12505)
                     /gene="mutL"
                     /locus_tag="OO332_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5943112.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12515..13852)
                     /gene="amiB"
                     /locus_tag="OO332_002688"
     CDS             complement(12515..13852)
                     /gene="amiB"
                     /locus_tag="OO332_002688"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5943113.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13871..14332)
                     /gene="tsaE"
                     /locus_tag="OO332_002689"
     CDS             complement(13871..14332)
                     /gene="tsaE"
                     /locus_tag="OO332_002689"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5943114.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14304..15851)
                     /gene="nnr"
                     /locus_tag="OO332_002690"
     CDS             complement(14304..15851)
                     /gene="nnr"
                     /locus_tag="OO332_002690"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5943115.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15850..16989
                     /gene="queG"
                     /locus_tag="OO332_002691"
     CDS             15850..16989
                     /gene="queG"
                     /locus_tag="OO332_002691"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5943116.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16972..17025)
                     /locus_tag="OO332_002692"
     CDS             complement(16972..17025)
                     /locus_tag="OO332_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943117.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3899 a         4925 c         4342 g         4088 t
ORIGIN      
        1 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
       61 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
      121 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg caaggttagt
      181 cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg ccgtagcgtt
      241 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg cgtccgcact
      301 acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca gcaggaacgg
      361 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac gtcctggctt
      421 cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga gatattggcg
      481 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
      541 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
      601 cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
      661 cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata tattgaattt
      721 gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaagcgcatt
      781 ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc cacttttttc
      841 ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc cgatggcttt
      901 ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc accgcggaag
      961 gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc gccttttttg
     1021 cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg cggtgcgcgt
     1081 tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc ttcgacgcga
     1141 acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat caggcgttgc
     1201 cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac cagaccatca
     1261 atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga aattacgcct
     1321 ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc agcgacatcg
     1381 cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag ttgcaacatc
     1441 tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg cccctgctct
     1501 ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg acgaatcggc
     1561 gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt ttctggatcg
     1621 taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc atcaggacgg
     1681 tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt accacccggc
     1741 agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc ggtgctcggc
     1801 ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg cgccgccgag
     1861 atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg ctgggtctgt
     1921 tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc gctctcaaat
     1981 gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt atgcaactct
     2041 tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc ctgcagaata
     2101 tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc gtagaatttg
     2161 tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca caggcggtct
     2221 acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat cgggataacc
     2281 tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag cggcgttggc
     2341 ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc gccgccgcgt
     2401 tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc aatggtgacc
     2461 agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac ttcttctttc
     2521 agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc atgggtacgc
     2581 agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc gacgatttta
     2641 cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac cacaaagccc
     2701 atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag acggctatcg
     2761 tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat ctggctggtt
     2821 tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc agcacccagc
     2881 ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct ggagagatac
     2941 aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg gccaataacg
     3001 gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg agtgaagacc
     3061 agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag ctgctcttcg
     3121 ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc acgtttggtt
     3181 aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc agcttcgcgt
     3241 ccctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat gaagattttc
     3301 gtcactccaa cagcaataat ttataaagcg gttgattctc ttcaaccaaa tcggcaagcg
     3361 tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc tgcttcaacc
     3421 gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat aagggctcca
     3481 gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg cgaatgccgc
     3541 cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg attattttga
     3601 ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg gtcatccgcc
     3661 cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg aaactcgtta
     3721 actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg atgataaacc
     3781 agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc gaggatttaa
     3841 cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac tgttagcccg
     3901 gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg cgcagaatca
     3961 tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg gtcagctctt
     4021 caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct ttcacgccga
     4081 aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct ttccagtcgt
     4141 cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa gccacgcaga
     4201 gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag ccagacaggg
     4261 agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta cgacgacgac
     4321 gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca gtttcatcaa
     4381 acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg aggataccca
     4441 gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca ccagcagtgg
     4501 tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc gtaccctgcg
     4561 caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg tcagaaacgt
     4621 caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga actttctggt
     4681 aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat tccatcactt
     4741 ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc agaccgcgac
     4801 gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg atcgctttcg
     4861 cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg atcagcggac
     4921 atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc agttctttca
     4981 tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg ctggttacat
     5041 tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg ttgattacga
     5101 gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc cgttcagtca
     5161 gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt acgacgacgt
     5221 tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat cgctttttca
     5281 gagttacagc actttttgca ctcaaaaatg acctcgaaca gtctgttttt tgttcagcca
     5341 atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc accgccaaaa
     5401 cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt ccatgccttc
     5461 gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc cagccagatt
     5521 gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct gaggatgcgg
     5581 tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt cggcgtcttc
     5641 atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt gccagagaag
     5701 ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc tttactgaat
     5761 gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat gcggccctga
     5821 cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg cagtttttcc
     5881 gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc ggcgcgcata
     5941 cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat acgcacatcg
     6001 acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac tttgcccttc
     6061 gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc cggggtagta
     6121 acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc gagggtcaga
     6181 cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc agaacgcaga
     6241 cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc accacccgtt
     6301 gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc gacgatcagg
     6361 tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg aatacgtgcg
     6421 tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg ctcataaacc
     6481 aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat accacgctca
     6541 ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat gataatcgcg
     6601 ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc tgcgcgttgg
     6661 cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt gctccactgg
     6721 ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta tcgctcttcg
     6781 ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc aggttgccac
     6841 ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc gtctcgatat
     6901 acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt ttagcaaagc
     6961 gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc gcctcttcga
     7021 ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac gcttctgctt
     7081 cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac gccgctttta
     7141 cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata cccatgtcat
     7201 acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg gtacgacctt
     7261 ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg ctgtcggtag
     7321 cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc ggattggtga
     7381 cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc agcatcacac
     7441 cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg tcgataaacg
     7501 tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag cgtgttacca
     7561 cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata atgacaatcg
     7621 ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct tgcgatgaac
     7681 tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt ttgctcagtt
     7741 tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg tttccatttc
     7801 cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt ccgttattac
     7861 cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc cctgttgtct
     7921 ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat caggtaatcg
     7981 atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat acgaacttgc
     8041 agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag ctgataaaaa
     8101 cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc cacctcgccg
     8161 gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt ctgtgcggaa
     8221 agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc gaaatcttcc
     8281 agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc gatctcttca
     8341 agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc atcaatgacg
     8401 tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc tgccaccaga
     8461 tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc accgacatct
     8521 gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc tgccgcgtag
     8581 acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt atatcccacc
     8641 agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc acgctgcttt
     8701 tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa acgacggtcg
     8761 gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg tctttcaagg
     8821 tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag ctcaacctgc
     8881 aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag gccggtgcgg
     8941 tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg gctcagggca
     9001 tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat ttcaactgct
     9061 ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt aatcacctgc
     9121 aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc ttccatatct
     9181 ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc ataacggtca
     9241 aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg gctccccgtg
     9301 taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc ggaagtattc
     9361 tgtgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt gttactgtga
     9421 tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac catctggctg
     9481 accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc ttgcagctta
     9541 ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc gttcaggaac
     9601 ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct tatttgtact
     9661 ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac acattcagcc
     9721 tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt tttcactgtc
     9781 aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg ccaactgtct
     9841 cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt caaggtaaga
     9901 ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca aatctcctcg
     9961 ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat gaaaacgctg
    10021 ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact gatgcacctg
    10081 atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac ccgaaataaa
    10141 aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc ttgctgccgc
    10201 aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct ctgccgcctg
    10261 ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca acccttccag
    10321 caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc ccgccgcggt
    10381 gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt aagcctgcga
    10441 cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag cgttcggctt
    10501 cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta tcaactctac
    10561 tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg tcggccccat
    10621 caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc agggctttta
    10681 tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc acaagttgcg
    10741 gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac cactgcgcat
    10801 gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg aatacggact
    10861 gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt tggcgtaagg
    10921 gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca atacccaatt
    10981 ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa acttttagcc
    11041 gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc aattgtgcct
    11101 gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca tcgcgctcca
    11161 gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga ctgttcgccg
    11221 caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg ggcgtttgca
    11281 aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca ttcggccagg
    11341 gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct accggctcac
    11401 gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc gggatggcac
    11461 gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc tgttgtagca
    11521 cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg aaacgcactt
    11581 cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc agatacaaca
    11641 caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg atcgcatgat
    11701 tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt tccgccagtg
    11761 cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg ccgtgttgcc
    11821 attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct aagcgccgtt
    11881 ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg ttatgcgaca
    11941 ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca tcaatatggc
    12001 taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg tagaacagat
    12061 ccagcacctc cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc acgtccatgt
    12121 cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt gaagtgagcg
    12181 tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc aggctgataa
    12241 tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc agcgccagcg
    12301 ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt ttcgccccac
    12361 cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt tccactagtt
    12421 ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg ttcgccagtt
    12481 gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg cgatctggcg
    12541 tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc atcggatgcg
    12601 caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt tggtaatcgt
    12661 cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg accagtactg
    12721 atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga cgtttatgta
    12781 tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac cctacccgct
    12841 gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca gactgactgt
    12901 tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc tgctccagcc
    12961 agctggccat ttcactgttg gcgcgacggt tagagagcac caatacggaa gcgccagtcg
    13021 cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg ttttgcttat
    13081 gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg gttaaaacgc
    13141 ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg gcgatggtga
    13201 catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg ccgtgtccgg
    13261 catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct gccggacgcg
    13321 ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta aacggattgc
    13381 gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt ttcgcaacca
    13441 cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg acagtgtaat
    13501 tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga tcgaccacca
    13561 gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc agattattgc
    13621 cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg agcgccacgg
    13681 tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc aacgttatcc
    13741 gtgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca cccaccggcg
    13801 tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc atcccgccac
    13861 ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag gaaaccgcac
    13921 tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca tccgggtcag
    13981 gaagaacacc tgtaccttgt tgtggccact ccacaaggca gatggcatcg ttggcaaaat
    14041 aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac aaatcaaagt
    14101 gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg gggcttttga
    14161 cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt ttacctgcgc
    14221 ctaaatcgcc atacagatag attacggttg ctccatcgca ggctttcgct acccgctcgc
    14281 ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc atggttttta
    14341 tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt tgccagcatc
    14401 ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc gacacagcct
    14461 gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat aataccagag
    14521 agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat aatgcctaaa
    14581 gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac gccgccataa
    14641 cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc gacggaacag
    14701 cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg acgcttatcg
    14761 ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt gcgaaaattc
    14821 tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg accaatcacc
    14881 accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc atgcaccatc
    14941 aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag tactcggact
    15001 aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc cgccgtgccg
    15061 tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt cgggcgacgc
    15121 ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt ctcctgacct
    15181 gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac atcccgcgct
    15241 tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc ggcgttgatc
    15301 actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc aaccgccaca
    15361 atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc gcggggcgct
    15421 tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc gggccagaca
    15481 atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc cagcgcggcc
    15541 tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat gccgaccgct
    15601 ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc gcacagcacc
    15661 agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc ctcgccagcg
    15721 cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg ttctccgcgg
    15781 cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt cttcattgta
    15841 tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat taaacagtgg
    15901 gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag cgagtccgag
    15961 cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg gatggcacgt
    16021 cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg tgtaatcagc
    16081 gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa aaaccccaaa
    16141 ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct gcgcaaccga
    16201 ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt tagaccgttt
    16261 gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct cggctggaca
    16321 ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt aggcgaattg
    16381 ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg caaatgcgtg
    16441 gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt cgatgctcgc
    16501 cgctgtatct cttatctcac catcgaactt gaaggggcga tcccggaaga gttgcgaccg
    16561 ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc gtggaatcgc
    16621 tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca cgcaccggaa
    16681 ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga aggatcggcg
    16741 attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt aggcaatgcc
    16801 ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca cccacttctc
    16861 gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc gtgcatagtc
    16921 gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg gttaccgcgt
    16981 gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa ttcaagacga
    17041 ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac ttatttatca
    17101 ataggttaaa aaataccatt caccgtgcga agtttctttg aaattaagat tacagcctga
    17161 ggctgtggat aactctgttt acaagagttt gtcagagaca acaaaaacca gcaataacgc
    17221 atcgcgtcgc tgtggatatt ttattgagag aaga
//
LOCUS       DAJTRQ010000080        17039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000080 DAJTRQ010000000
VERSION     DAJTRQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..171
                     /locus_tag="OO332_002693"
     CDS             <1..171
                     /locus_tag="OO332_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5943118.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(172..363)
                     /locus_tag="OO332_002694"
     CDS             complement(172..363)
                     /locus_tag="OO332_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5943119.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(468..704)
                     /locus_tag="OO332_002695"
     CDS             complement(468..704)
                     /locus_tag="OO332_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5943120.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(822..2261)
                     /gene="rsmF"
                     /locus_tag="OO332_002696"
     CDS             complement(822..2261)
                     /gene="rsmF"
                     /locus_tag="OO332_002696"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5943121.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2341..4839)
                     /gene="yebT"
                     /locus_tag="OO332_002697"
     CDS             complement(2341..4839)
                     /gene="yebT"
                     /locus_tag="OO332_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5943122.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4808..6092)
                     /gene="yebS"
                     /locus_tag="OO332_002698"
                     /pseudo
     CDS             complement(4808..6092)
                     /gene="yebS"
                     /locus_tag="OO332_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6222..6719
                     /gene="msrC"
                     /locus_tag="OO332_002699"
     CDS             6222..6719
                     /gene="msrC"
                     /locus_tag="OO332_002699"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5943123.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6816..7514
                     /gene="proQ"
                     /locus_tag="OO332_002700"
     CDS             6816..7514
                     /gene="proQ"
                     /locus_tag="OO332_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5943124.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7534..9582
                     /gene="prc"
                     /locus_tag="OO332_002701"
     CDS             7534..9582
                     /gene="prc"
                     /locus_tag="OO332_002701"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5943125.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9774..10655
                     /gene="htpX"
                     /locus_tag="OO332_002702"
     CDS             9774..10655
                     /gene="htpX"
                     /locus_tag="OO332_002702"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5943126.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10701..12074)
                     /locus_tag="OO332_002703"
     CDS             complement(10701..12074)
                     /locus_tag="OO332_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943127.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12251..13042
                     /gene="kdgR"
                     /locus_tag="OO332_002704"
     CDS             12251..13042
                     /gene="kdgR"
                     /locus_tag="OO332_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5943128.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13185..13424)
                     /locus_tag="OO332_002705"
     CDS             complement(13185..13424)
                     /locus_tag="OO332_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943129.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13583..13726
                     /gene="mgrB"
                     /locus_tag="OO332_002706"
     CDS             13583..13726
                     /gene="mgrB"
                     /locus_tag="OO332_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5943130.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13801..14088
                     /locus_tag="OO332_002707"
     CDS             13801..14088
                     /locus_tag="OO332_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5943131.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14758..14901
                     /locus_tag="OO332_002708"
     CDS             14758..14901
                     /locus_tag="OO332_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5943132.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14914..15123
                     /gene="cspE"
                     /locus_tag="OO332_002709"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14914..15123
                     /gene="cspE"
                     /locus_tag="OO332_002709"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5943133.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15289..16098
                     /gene="rlmA"
                     /locus_tag="OO332_002710"
     CDS             15289..16098
                     /gene="rlmA"
                     /locus_tag="OO332_002710"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5943134.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16095..16661)
                     /gene="mntP"
                     /locus_tag="OO332_002711"
     CDS             complement(16095..16661)
                     /gene="mntP"
                     /locus_tag="OO332_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5943135.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4497 a         4127 c         4305 g         4110 t
ORIGIN      
        1 tgtagccgct cgcgattagg tgaacgccca aaagggcagg gaattacagg tgctgatcat
       61 ttctggtttg gtcatacacc gttgcgacat cgcgtggata ttggcaacct gcattatatt
      121 gataccggtg ctgtctttgg gggcgaactg actcttgtgc aactgcaata attaaaaatc
      181 accatactcc tgtgctggtc gccagaaacc atctataaaa tcctcaatcg gaaaacaacc
      241 gccatggcgg atccgttgat cgctcataga ataaagacac tgctgttccg tgttgtagac
      301 atccacaaca atatcttcac aaccgccatc caggtagcaa acaaaaagta ccagcgcgaa
      361 catttcatcc ccgaagtgtg gtgccgtacc gttaagttta ggagagattt tacaacgggg
      421 gaataaccag gacaaataac ccgccatgcc gacgggttct ttttggatca ggcaagacgc
      481 ataatcctgg catcagattg ttgatcgtaa cgggtacggt aaaggacaga gtgttcgcca
      541 tttagcaggg cagggatact ggtttcagca tcccaggcat ccagttgcat acataaatcg
      601 gggtcagatt tacaaggaat ggttaacgtt cgatcacccg gcgattcacc gtgtaattcg
      661 ccgtcgtcga tttcaaaagc gcctatgcgc acactggttt tcatcttcgt gctctccatt
      721 tagctgctga ttgtggtacg accagtccga tcgcacaatt tttcagccta gttgaggccg
      781 ccgaatgtgc cagtcaaaaa gtgcgctttt tttgtgcgct gtcatgcgtt actggtaaaa
      841 agtttcccgt cccgcaccag ttcacgcggg tagctgtttt tcagtcgcga accaatccgt
      901 ttggctaaac caatcggctg atgctggaaa gtaaccaaca cgtcatccgc cactggcgcg
      961 gcttgcgggt aaacatcgcg cccgcgatac cactcctccg cttcctgcgg tgtcagttca
     1021 aaagcgttta cattgtcggg ggaggcatgg gcaataaccg cttcatgctg ccagcgataa
     1081 cctttgttat gcgtttcggc aagtttaatc cctaagcgag aaaatcggac tttaccgatc
     1141 agggcttcaa tgcctaccgg gaacaaccac agttctttgt cgcgttgcca gagtcgcagg
     1201 tttccatccc agtttaagcc aacacttgca gccgcctgac gaatttgtcc ggcttcgcga
     1261 tctttcaccg ggctgaacgg gaaattgccc actttgtatt ttggggcggg taaggcgggg
     1321 atcgcctgag ttttacgcag acgagcaacg aagaagcctt cgcagtcgta aatttgtggg
     1381 aaaacatgca aaaaaccttc ttcggtcagc gccttgtttg caccagggaa gagatcgcca
     1441 agtggtaaaa actctacttc gtcggggtaa gtctctttca gccaccggca aacggcttcg
     1501 ttttcttccc ggtttaaggt acaggtcgag taaaccagcg taccgccagg acgtaatgca
     1561 tgaaaggcgc tgtcaatcag ttcccgttgg gtagctgcga tttcctgatt gctttcgggt
     1621 gaccagtttt ttagcgcatc gggatcttta cgcaccacgc cttcgccgga gcagggagcg
     1681 tccagcaaaa tggcatcaaa catttctggc actgacgcac caaacacgcg gccaccaaaa
     1741 tgtgtgagcg caacattact gatgccacag cggctgatat tggcatgtaa cacttttacc
     1801 cgactggcgg aaaactcatt ggcaaggatt gccccttcgt tattcatccg cgcggcaatt
     1861 tgcgtcgttt ttgagcctgg cgcagcagcg acatccatca cccgctgtgg tgcattaccg
     1921 tcagcaaaca aggcggcgac gggcaacatt gaactggctt cctgaatata aaacaggcca
     1981 cttaaatgct cggtggtact acccaatggc aatgcatctt cattgtcgcg ttcaatccag
     2041 aaaccttctt cacaccacgg aattggcgta agcgtccagc cataaggagc ggttaattgc
     2101 aggaaatcag caacggagat tttcagcgta ttaacgcgaa tgctgcggcg caacgggcgc
     2161 tgacaggcgg caagaaaatc atcaaatgag agcgtcgaag gcatagcttc gcgcatttgt
     2221 gtcagaaacg cgtccgggaa ataaacggcg tgttgggcca cgagcatgta ccactggcaa
     2281 aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga gcagtgggca
     2341 ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt cctgcaacag
     2401 gaagtgtttg ccttcctggg ctttcggtgc aagtggcgta cccggaggcg tggcgaaggc
     2461 gatgccgcca cggataaatt ggttaaaggt gccggttttc actacgccgc ccgtcagacc
     2521 aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca ccaggtgttg
     2581 atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc ccagcgtcat
     2641 tccggtaacc gtacctactt ccagaccacg gaacagcaca ggcgtaccga tgcctaacga
     2701 accggcttcc ggcgcttcaa caataatgct taaaccatct aggtaacgcg aatcagtaat
     2761 ggtggcctct tctaattcaa agtcgcgacg aggattgcca cggcctggtt cgacgttgat
     2821 atacggctgg aggatagtat ctagatgctc aacgccagct gccgagattt gcggtgtgac
     2881 cactgagaag cgcgtaccac cgcgagcaaa ggtctggaca tattccggat agagcaccgc
     2941 ctttgcctgt acttcattgc gtgcggtaat cagatccagc gtctggattt gcccgatatc
     3001 aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga aagcgtgaag
     3061 cgtaatctgc ctgccaaccg cacgggccgc tgtttcggaa gcatacagaa ttcgcttgtc
     3121 gcctttacgc tgactggcgc tggcaccact gaggttatcg aagctaatgg ccccctttaa
     3181 tgctctggag agcggggatg cctgtacggt caggccacta ccattcagct gaactttcgc
     3241 cccgccttct gcccagaaca cgctgttgct ggtcaaaagg ttgcgatatt ccggtttaat
     3301 gtgcagatcg atatcgaacg cgttagctcg cggacgcacg gtaataactt caccaacttc
     3361 aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct ctgcactcaa
     3421 actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt ccagattggc
     3481 atacagtgga tagctggctt tcatctcgcc tttatcgccc ggcagaatac gtatcccgcc
     3541 gttaatccat tctgaggcgc tggcaccgag aaactcaacg ccatccagcc ccaccttcac
     3601 gtcgacacgg ctgttgacga caaatttgct atcgcctttt accagttctc gatgctgagg
     3661 ctcgatggcg acggtaaagg tgacgccttt gctggtgagt ttacgatcga taacctggcc
     3721 tacctgcacg ccgtgaagaa tgagcggctg acccgcatca ataccgtaac tttccggtgc
     3781 tgtcagggtc agtgtcagaa cgtccggctc ttgcagcagg gctttttcgc ccggcacaac
     3841 aacgaactct ttgcgtggct cgccatcgcc gggcaccaac tcaaaggttt ttccggtcag
     3901 cagggcgctg agattagcat cgctaaggga taatttcggg ttgcgtaatt ctatgcgggt
     3961 attttcacga agcagggtaa cgacgctggg atcaacggtc atttccccag tgactttacc
     4021 accaggattt aaatccagtt tagtcagctg tccgacttcc agcccctgat acattaacgg
     4081 cgtcgagtcg gcggttaatc ctgctccact cggcagttcc agtttcacct tcgcgccact
     4141 gatactgacg ttggcatcaa cgccggaaac gttccagaaa cggctacctt ttttcaccag
     4201 atcggtaaaa cgccgctcga tcaggacatc aatcaccacg ccttgcttgt tgggattgat
     4261 ggcatagtcg tagacttttc ccaccgggat cttgcggaaa tagaccaatg aaccgctgtt
     4321 cagcgaaccg agatcggggg cttgcaggtg gatcatcaga tcgccattgt ccagccgata
     4381 tttcggttgg gtatcgagcg cgacaaagtg atcctgctct ttacctttac ccggcatcat
     4441 gccgatatag ttcccaccga cgagggcgtc cagcccggag acacctgcca acgatgcttt
     4501 tggcgtcatc agccagaact gagtctcttc gcgcagcgca tctttcatat cggacttgat
     4561 gctgaccttg acttcaatct tacgaagatc gtcgctgagg ctgatatcct gcactgttcc
     4621 gacttcaacg ccctgataac gaacaggcgt acggcccgga acaataccat ccgccgacat
     4681 aaagtcgatg gtgatggtat taccccggtc ctgataactg tcccaaatca gccaaccggc
     4741 aatcattagc gcgatgaaag gcagcagcca gaaaggtgag atacggcgtt tatttttaat
     4801 ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg tgcatcccaa agtaagcggc
     4861 tgtccagcca ttccacagca agaatagtca aaattacggc agcgccaaaa taaaacgcag
     4921 ccggtcccat agtaaaagcg aggatctgat cgcgattaat cagcgacatg gttaaagata
     4981 tgacaaacag gtcgagcatc gaccagcgtc caatccaggt caccatccgc agtaacagaa
     5041 tgcgtgtgcg taaaccttgc tggcatttaa aatgaatgct caacagtaaa gtgaacatga
     5101 cgatcacttt agtaaacggt accagaatac tggcgataaa cacgattccc gcaaccgcaa
     5161 tgttgctact tgccagcgac ataattccgg aaagaatcgt atcttcctgc cggccaccat
     5221 tcagataaat gatagaaata ggcaataggt tggctggtaa caacaaaacg attgacgcta
     5281 acagcgccgc ccagcatttt tgcaaactat gacgacgacg caggcgtagc gggatatggc
     5341 aacgcgggca gagaccacgc tgatcaggat agccggtaaa atggcaccca agacagacac
     5401 gaagtttctc gtccctacgc gtagcggggc gctgcggata atatcgctcc cacagctcct
     5461 caacattaag atgtgacaac gtcaccgtcg ttaaaatcac caacgccaca aaagagaaca
     5521 agccgacacc cgcctggata tgggcataat cctgtacctt atatagaagc aacgccaatg
     5581 ccgaccaggt agatatccag cattacccac tctttaagtc gctcaagcat cagcagtacc
     5641 ggacgtaaat tcattcccag tcggttacca aaccataaat atgctatgga ggacaccaga
     5701 atgagggggg caccaataac gcagaaaaag accattgatc ccgttatcgc atcgccctgt
     5761 ttggtcattt gccagatgcc ttgcatgacg ttggcgtcaa tacgaatgcc taacagccag
     5821 atatgcaaca gcggttcgcc ccaggcaaac ggcataagca acagcatggt gaaggccatt
     5881 gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa tttttgcctg acagcgcgga
     5941 caataggcac tttgatgaga atttatctcg ggcaggctaa acagcatgtc acattgcggg
     6001 caacgttggt aatcaccacg cggcagttcc tcgccgattg ccctcactgt tatctttttt
     6061 gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc aaatgtaata agaattaaca
     6121 ttaatcttaa cccatgatgg gaatgatctt gagcattaac gcatttaatg cttattttaa
     6181 taggctggca gttccaggta agacaaccaa gtgattaaat aatgaacaaa acagaatttt
     6241 acgcggattt aaatcgcgac tttaacgcgc tgatggcggg agaaaccagt tttctggcaa
     6301 cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac tgacgttaac tgggcaggtt
     6361 tttatttgct tgaggacgat acactggtac tcggaccatt tcagggcaaa attgcctgtg
     6421 tccggatacc cgtcgggcgc ggggtgtgcg gcacagcggt tgcccgcaat caagtgcagc
     6481 gtatcgagga tgttcacgcg tttgacgggc atattgcctg tgatgcggcg agtaattctg
     6541 aaattgttct gccgctggtg gtgaaaaatc agattattgg tgttctcgac atcgatagta
     6601 ccgtcttcgc tcgctttaca gacgaggacg agcaaggctt acgtcagctt gtggcacagc
     6661 ttgaaaaagt gcttgcaacg acggattaca aaaaattctt tgcgagcgtc gcaggataat
     6721 caacggataa cgtagcattt actggtagcg tcattataat gacgcctgtt catgcctgcg
     6781 cttgttggct acgtccgttg taatcaggaa atttcatgga aaatcaacct aagttgaata
     6841 gcagtaaaga agtaatcgcg tttctcgccg aacgttttcc ccactgtttc agtgcggaag
     6901 gtgaagcgcg tccgctgaaa attggtattt ttcaggattt ggtcgatcgt gttgccgggg
     6961 aaatgaacct gagcaaaacg caattacgat ccgctttacg tctctacact tcgagctggc
     7021 gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg
     7081 gtgagctgga cgagcaacat gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc
     7141 gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg
     7201 gtgagaaaga agacgcaccg cgccgcgaac gcaagccacg tccgaccacg ccacgccgca
     7261 aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag
     7321 taaaagcacc tcgcgaagaa cagcacaccc cggtttctga catttcagct ctgactgtcg
     7381 gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca
     7441 ccaaagacgg cgtccgcgtc cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac
     7501 acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca tgttttttag gcttaccgcg
     7561 ttagctggcc tgcttgcaat agcaggccag accttcgctg tagaagatat cacgcgtgct
     7621 gatcaaattc cggtattaaa ggaagagacg cagcatgcga cggtaagtga gcgcgtaacg
     7681 tcgcgcttca cccgttctca ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa
     7741 atctttgacc gctacctgaa tctgctcgat tacagccaca atgtgctgct ggcaagcgat
     7801 gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc
     7861 gacgttttct acgatctcta caatctggcg caaaagcgcc gttttgaacg ttaccagtac
     7921 gctttgtcgg tactggaaaa gccgatggat ttcaccggca acgacactta taaccttgac
     7981 cgcagcaaag cgccctggcc gaaaaacgag gctgagttga acgcgcagtg ggacagtaaa
     8041 gtcaaattcg acgagttaag cctgaagctg acaggaaaaa cggataaaga aattcgtgaa
     8101 accctgacac gccgctacaa atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat
     8161 gttttctcgc tggcaatgat ggcgtttgcg cgtgaaatcg atccgcatac caactatctt
     8221 tccccgcgta ataccgaaca gttcaacact gaaatgagtt tgtcactgga aggtattggc
     8281 gcagtgctgc aaatggatga tgactacacc gttatcaatt cgatggtggc aggtggtccg
     8341 gcagcgaaga gtaaagctat cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc
     8401 aagccgatgg ttgacgtgat tggctggcgt cttgatgatg tggttgcctt aattaaaggg
     8461 ccgaagggca gtaaagttcg tctggaaatt ttacctgctg gtaaagggac caagacccgt
     8521 actgtaacgt tgacccgtga acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg
     8581 aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc cgggcttcta tgtgggtttg
     8641 acagacgatg tcaaagttca actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc
     8701 atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag cggtatcgct ctcaggtctg
     8761 tttattcctg cgggtcccat tgttcaggtc cgcgataaca acggcaaggt tcgtgaagac
     8821 agcgataccg acgggcaggt gttctataaa ggcccactgg tggtactggt tgaccgtttc
     8881 agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg attacggtcg tgcgctggtt
     8941 gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat accgttcatt gaaccgtatt
     9001 tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt ctgtgcagta cacgatccag
     9061 aaattctatc gcgttaacgg cggcagtacg caacgtaaag gcgtaacacc agacatcatc
     9121 atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg
     9181 tgggatagca tcgatgccgc gacttatgtg aaatcaggag atttaactgc ctttgaaccg
     9241 gagctgctga aggaacataa tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg
     9301 aaggatatcg cgcgcttcaa cgctatgaag gacaagcgca atatcgtttc tctgaactac
     9361 gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc gtctggcgcg tttgaacgaa
     9421 cgctttaaac gcgaaggtaa accggagttg aagaagctgg atgatctacc gaaagattac
     9481 caggagccgg atccttatct ggatgagacg gtgaatatcg cactcgatct ggcgaagctt
     9541 gaaaaagcca gacccgcgga acaacccgct cccgtcaagt aatatcaatc aggcacaaga
     9601 aattgtgcct gattttttaa cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa
     9661 agttgtgtct ttctggtgac ttacgcacta tccagacttg aaaatagtcg cgtaacccat
     9721 acgatgtggg tatcgcatat tgcgttttgt taaactgagg taaaaagaaa attatgatgc
     9781 gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt cgttttcggg ctggtactga
     9841 gcctgacagg gatacagtcg agcagcgttc aggggctgat gatcatggcc ttgctgttcg
     9901 gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg gatggcatta cgatctgttg
     9961 gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg ttggctggtc aatactgtag
    10021 caacccaggc tcgtcaggcg gggatcgcta tgccgcaagt ggctatctac catgcgccgg
    10081 acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg
    10141 gtttgctgca gaacatgagc ccggatgaag ccgaggcggt aattgctcac gaaatcagcc
    10201 acatcgccaa tggtgatatg gtcaccatga cgctgattca gggcgtggtg aacaccttcg
    10261 ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg
    10321 atgaaggtga agagagcaac ggcaacccgc tgatctactt tgcggttgca acggttctgg
    10381 aactggtgtt tggtattctg gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat
    10441 tccatgctga tgccggttcg gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc
    10501 agcgcctgaa aaccagctat gaaccgcaag aagcaaccag catgatggct ttctgcatta
    10561 acggtaagtc gaaatcgctc agtgagttgt tcatgactca cccgccgctg gataaacgta
    10621 ttgaagctct gcgtacgggt gaatacctga agtaatatga agtaaatact aaaaagcgcg
    10681 tcctgatcga cgcgcttttt ttatgccctg gctcgtggct gagtgatacg taaaccactg
    10741 acacaggcag caatcactgc cagaatagcc gcagccatca gcgagacgtg cgtaccatta
    10801 tcaccaaact gatttagcat cagcgccacc agcgccgcgc cactactctg acccagtaga
    10861 cgagccgttc ctaacatgcc actggcccca ccgctacgtt cgcgcggagc ggaggtaata
    10921 atggtgtggt tattgggtga ctggaataag ccaaatccag cgccacataa gatcatcggc
    10981 cagataatat tgatatccgc aggtgacgcg ggcagcagaa ccagggaaaa aagccccgca
    11041 gccatgatga acaaccctaa agcacccagc aatcctgcat gtacgcgttc aatcaaatag
    11101 cctgccagcg gagccatcac catcgttgct aacggccacg gtgtcagaag taaacctgtt
    11161 tcgacttcac tacgcccgag cacggtttgc aggtaaaagg gcagggaaac cattgccagc
    11221 atttgtgcgc agaaagagca aacagatgtg caaatagaaa gtgaaaacag cgggatacgc
    11281 agtaaatcca ccggtagcag cggtacggga agagaaagct ggcggcgaat aaagaaaata
    11341 ccaacaacaa ccattaccac cagttccgca gcaattaacg tcagcgattg cccctgagcg
    11401 aaaccactca acgcagtgat aagcaggccg aaggttaacg cgttcatcac ggcgctgggc
    11461 aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga
    11521 agcagggcga taatacctaa cggtacgttg attaaaaata accatttcca ggatgcgatg
    11581 gagaggattg ctgcagcaat tgtcggcccg gcagcagaag agacggcaac aataaacgag
    11641 tttatgccca tccctctccc cagaaaacgt tgtggataga tcaggcggat aagtgcggta
    11701 ttaacgctca tcaacgctgc accgccgaaa ccttgtatga cacgcgcaag ggtgagcatt
    11761 tgcagcgaat cagaaagggc gcagaacagt gaagacaaca gaaaaacgac cagaccgcat
    11821 ttataaatac gtcgatagcc aaacatatcg cccagaaacg aaaacgagag cagggagatg
    11881 acaatggcga tttgataggc gttcactacc cagatggaac tggctggtgt ggcatgaagg
    11941 tccgtggcga ttgttggcag ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa
    12001 ataccaatca caatggttaa tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc
    12061 tgaacttttg gcataggaat tttatatctt tggtgaataa tcaacagggg tatgattcta
    12121 gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg
    12181 accaccctgg tgcgaaatta taataaaaaa cagttctgat ttttataaaa cactcgcaat
    12241 gaggtgataa atggctaacg cagatctgga taaacagcct gattctgtat cttccgtgct
    12301 aaaagttttt ggcattttgc aggcgctggg tgaagagcgc gaaataggga taaccgagct
    12361 gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc tttttacaga ccatgaaaac
    12421 cttaggttat gtggcgcagg aaggggagtc ggagaaatat tccctgaccc tgaaattgtt
    12481 tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt cgtagcgcag atatccagat
    12541 gcgtgagatc tcccgcctga ccaaagaaac tatccacctc ggcgcactgg acgaagacag
    12601 tattgtttac attcacaaaa ttgactctat gtacaatttg cgcatgtatt cacggattgg
    12661 gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg
    12721 cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa cgcagtaccg agcggaccat
    12781 caccagtaca gaagcgttat tacccgttct ggaccaggtg cgcgagcagg ggtatggcga
    12841 agataacgaa gagcaggaag aagggctgcg atgcattgcg gtaccggtat ttgatcgctt
    12901 tggcgtggtc attgccggtt tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg
    12961 tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg
    13021 ttatcacgac tatccgttct gatgagagta agaacctgtc ggaatatcaa acagacaggt
    13081 tctttattta gcatgagaaa aataaagttg aaggtgcgtt atattaaacg cgcttgctat
    13141 aagaatattt tactcatgag tgagaatctg gttatttatt acccttaacc attatcgacc
    13201 acgatattgc ttttacgtaa cagtgggcaa tctgttatcc ccaagaaacc acttttagtg
    13261 tgcaagtatt gtaccgtgct ggtgcctctg gccgtcagat aggtacattg caaacctaat
    13321 cctgcggcat tctctttgct tccaatcaaa acgccatatc cgctgagtaa taatcctatc
    13381 cataccagtg ctatcagcat aactgtgcga atgatgaatc gcattacaat ctcttctctt
    13441 tttatgttcg cttaatcgta gcggcaatat gcgctgaagc aagctactca ttccgaaaaa
    13501 gcacgaatat cgacatagtt aggcgctgtt taactaacgc atgctagttt aatgacataa
    13561 ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg gtcgttctgg ttgtcgtggt
    13621 gttggcttgc ttgctgcttt gggcgcaggt attcaacatg atgtgcgatc aggatgtaca
    13681 atttttcagc ggaatttgtg ccattaacca gtttatcccg tggtgatatc attttttcag
    13741 cactagattt tcttcctctc cagcagtggt agaatagcgc ctctactcag aggtggtgaa
    13801 atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt ctttggttgt atcggtggtg
    13861 gttcttctta tcgggctcat cttgtggttt tttatcaatc gtgccagttc acggactaac
    13921 gaacagattg aactgcttga ggcgttgctg gatcagcaaa aacgtcaaaa tgcactgtta
    13981 cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata aaaagaccat tgagagtcaa
    14041 aaatcggttg atgacgaaga tattattcgc ctggtcgccg aacgataatc cttcctcaat
    14101 cttgagtaac cccgtcgttg aagggttact caattcatcc tgattatacg cactgtcatg
    14161 gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa taattatcat attatttatt
    14221 tgcgtaacgg gatatcagaa aaagatattt tcctcattaa tttattagga tgtttacatc
    14281 agatttgtga ttaagcgtgg tattatttat tagcgaaacg tttctctctt gatttttttg
    14341 ctcactcatc aatttttctt attttaaatt tacaatccct ttggggattg acttctcttt
    14401 agggtaattg atagccgtta actgactgtt ttatgagaaa aagtgatata actttttatt
    14461 cattgcatag cagaaaatgt gatattgcac gcactatgta ataacttctc cctctggcct
    14521 ggaacaactg aacttattga actatgttag aaactacgcc agtttaagta tctgcctgaa
    14581 ctggcaaggt taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa
    14641 aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc
    14701 ataattaacg ttgtatgtga taaaagattt cgggttaaac gaggtacagt tctgtttatg
    14761 tgtggcattt tcagtaaaga agtcctaggt aaacacgttg acgttgaata ccgcttctct
    14821 gccgagcctt atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg
    14881 cgggcgaaga aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag
    14941 tggttcaacg agtctaaagg ttttggcttc attactccgg ctgatggcag caaagatgtg
    15001 ttcgtacact tctccgctat ccagggtaat ggcttcaaaa ctctggctga aggtcagaac
    15061 gttgagttcg aaattcagga cggccagaaa ggtccggcag ctgttaacgt aacagctatc
    15121 tgatcgaatc cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg
    15181 aatgcgaggc tttttactat gctttatctt cgctcctggc gttcggatat ttgccagcca
    15241 cgtgattcgc gttacacttg cggcctttag tatcctgccg gagttgtcat gtcgttttcc
    15301 tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg tccccaacga
    15361 catcagtttg atatggcgaa agaagggtat gtcaatctgc tgcccgttca gcataaacgg
    15421 tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc
    15481 ggacattatc agccgctgcg tgatgcaatt gtcgcccaac tgcgggaacg gcttgatgaa
    15541 aaggccacgg cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc
    15601 gatgcgttgc ccgaaatcac cacgtttggc ctggatgtct cgaaggtagc gataaaagcg
    15661 gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt
    15721 tccgatacca gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta
    15781 gcacgagtag tgaagcccgg cggctgggtc attactgcca cgccgggacc gcgacatttg
    15841 atggagctga aggggctgat ttacaatgaa gtacatcttc atgcacctca tgcagaacaa
    15901 ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt
    15961 gatgaagccg tcgcattatt gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc
    16021 tggcaaacac tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc
    16081 tggcagcgtt cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc
    16141 agcaccagcc cgccgagaat ttccgctttt ttcccaataa ttgagccgat aaagcgacca
    16201 accatcatcc ctaatgtcga cataatcaag gttgcacaac caatggccaa tgcggtcgcg
    16261 ataatgttga cctgaaggaa agcaagacca acacccacag ccatggcatc caggctggtg
    16321 gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca
    16381 tcatctgagc cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca
    16441 aacgcaatcc agtgattcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag
    16501 ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca
    16561 gaaaatttcg gtttatggag gggggcacct ttaccgattg atgcagcaaa tgcatccatc
    16621 gacataccaa acgcaagaag aacagtagca gtgatattca tgacaacgtc ctgaccgggg
    16681 tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac aagccgcgat
    16741 gggttttcgc gttcatgcag gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa
    16801 gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt tgacacgtac atttccggaa
    16861 tctggaaatc ggctactccc caaatgaggg cgcaacctta acatattttg agaatataaa
    16921 acaactgttg gaatatatta tttcattgct ttattgataa cgattttcat ttatgtttat
    16981 atatcaatga aacgttaaaa taaataacaa ataagggaag agataatata gcctaagct
//
LOCUS       DAJTRQ010000081        16819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000081 DAJTRQ010000000
VERSION     DAJTRQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..949
                     /locus_tag="OO332_002712"
     CDS             <1..949
                     /locus_tag="OO332_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCR5943136.1"
                     /translation="YLTGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEI
                     DPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETE
                     GTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPI
                     DLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKV
                     KARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKH
                     LVTPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            949..4098
                     /gene="mdtB"
                     /locus_tag="OO332_002713"
     CDS             949..4098
                     /gene="mdtB"
                     /locus_tag="OO332_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5943137.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFI
                     PKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTN
                     PSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRT
                     QYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLG
                     ISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGV
                     VPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVD
                     ITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGA
                     LLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLL
                     RFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLL
                     FDRLVLWTKSRFARHEEEA"
     gene            4099..7164
                     /gene="mdtC"
                     /locus_tag="OO332_002714"
     CDS             4099..7164
                     /gene="mdtC"
                     /locus_tag="OO332_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5943138.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7165..8580
                     /locus_tag="OO332_002715"
     CDS             7165..8580
                     /locus_tag="OO332_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943139.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8577..9980
                     /gene="baeS"
                     /locus_tag="OO332_002716"
     CDS             8577..9980
                     /gene="baeS"
                     /locus_tag="OO332_002716"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5943140.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9977..10699
                     /gene="baeR"
                     /locus_tag="OO332_002717"
     CDS             9977..10699
                     /gene="baeR"
                     /locus_tag="OO332_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5943141.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10890..11222
                     /locus_tag="OO332_002718"
     CDS             10890..11222
                     /locus_tag="OO332_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5943142.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11369..12730
                     /gene="yegQ"
                     /locus_tag="OO332_002719"
     CDS             11369..12730
                     /gene="yegQ"
                     /locus_tag="OO332_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5943143.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13072..13389)
                     /locus_tag="OO332_002720"
     CDS             complement(13072..13389)
                     /locus_tag="OO332_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943144.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13795..14694
                     /gene="yegS"
                     /locus_tag="OO332_002721"
     CDS             13795..14694
                     /gene="yegS"
                     /locus_tag="OO332_002721"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5943145.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14776..15549)
                     /locus_tag="OO332_002722"
     CDS             complement(14776..15549)
                     /locus_tag="OO332_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5943146.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15655..16695)
                     /gene="gatD"
                     /locus_tag="OO332_002723"
     CDS             complement(15655..16695)
                     /gene="gatD"
                     /locus_tag="OO332_002723"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5943147.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
BASE COUNT         3888 a         4503 c         4408 g         4020 t
ORIGIN      
        1 ttacctcacc gggcttggca ccattaccgc cgctaatact gttacggtgc gcagccgcgt
       61 ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag caggcgattt
      121 actggcagaa attgacccca gccagttcaa agttgcatta gcacaaaccc agggccaact
      181 ggcaaaagat aaagccacgc ttgctaacgc ccgccgtgat ctggcgcgtt atcagcaact
      241 ggcaaaaacc aatctcgtct cccgtcagga gctggatgcc caacaggcgc tggtcagtga
      301 aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga
      361 ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt ctcaagcaag ttgatgttgg
      421 taaccaaatc tccagtggtg ataccaccgg aattgtggtg atcacccaga cgcatcctat
      481 cgatttagtc tttaccctgc cggaaagcga tatcgctact gtagtgcagg cgcaaaaagc
      541 cggaaaaccg ctgatggtag aagcctggga tcgcaccaat tcgaagaaat taagtgaagg
      601 cacgctgtta agcctcgata accaaatcga tgccacaacc ggtacgatta aagtgaaagc
      661 acgctttaat aatcaggatg atgcgctgtt tcccaatcag tttgttaacg cgcgcatgtt
      721 agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc gccctgcaaa tgggcaatga
      781 aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc tggtgacgcc
      841 gggcattcag gacagtcaga aagtggaagt ggtggaagcc cagagcgcca ccactccgga
      901 agagaaagcc accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta
      961 cccccgagca gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg
     1021 ctgctgatgg tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg
     1081 gcgctgccgg aagtggacta tccgaccatt caggtggtca cgctctaccc aggtgccagc
     1141 ccggatgtca tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct
     1201 ggcctgaaac agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc
     1261 cagctaacat taccgctcaa tgtcgccgag caggaagtgc aggccgcgat taacgctgcg
     1321 accaacttgt tgccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca
     1381 gatccgccga tcatgacgct cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa
     1441 gatatggtgg aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg
     1501 acgctttccg gcggtcagcg tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc
     1561 gccctcggcc tgaccagcga aaccgtgcgc accgccatta ccggcgctaa cgttaactcg
     1621 gcaaaaggta gcctcgacgg cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg
     1681 caatccgccg aagagtatcg ccagctaatc atcgcctacc agaacggcgc gccaattcgt
     1741 ctgggcgatg tagcaactgt agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg
     1801 aacaaagaac aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc
     1861 accgccgaca gcattcggca gatgctgcca cagctcactg agagtctgcc gaaatcggtg
     1921 aaggtgacag tgctttccga tcgcaccacc aatatccgcg catccgtcga tgatactcag
     1981 tttgaattga tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat
     2041 attccggcga ccatcattcc cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg
     2101 gttatggtgt ttctcgattt ttcaatcaat aacctgacac tgatggcgtt aactatcgcc
     2161 accggattcg tggtcgatga cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa
     2221 aaaggcgaaa aaccgttggc ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt
     2281 atctcgctga ccttctcact gattgcggtg ttgatcccac tgctgtttat gggcgatatc
     2341 gtcgggcgac tgttccgcga atttgctatt accctggcgg tagcgatttt gatctcagcg
     2401 gtggtgtcgc tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg
     2461 cgtaaacaga accgcttctc ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc
     2521 tatggtcgtg gactggcgaa agtgctgaat catccgtggc tgaccttaag cgtggcactc
     2581 agcacgctgc tgcttagcgt ggcactcagc acgctgctgc ttagcgtgct gctgtgggtg
     2641 ttcattccga aaggtttctt cccggtacag gacaacggca ttattcaggg cactttgcag
     2701 gcaccgcaat ccagctcctt tgccaatatg gcccagcgac aacgccaggt cgcggacgtg
     2761 attttgcagg atccggcagt gcaaagcctg acctcatttg ttggcgttga tggcactaac
     2821 ccgtcgctga atagtgcacg tttacaaatc aacctcaaac cgttggatga acgtgatgat
     2881 cgggtgcaaa aagtcatcgc ccgtctgcaa acggcggtag ataaagtgcc gggcgtcgat
     2941 ctcttcctgc aaccaacgca ggacctgacc atcgatactc aggtcagccg cacccagtac
     3001 cagtttacct tgcaggccac gtcactggat gcgctcagta cctgggtgcc acagttgatg
     3061 gaaaaactcc agcaactgcc acagctttct gatgtctcca gcgactggca ggacaaaggg
     3121 ctggtggcgt atgtcaatgt tgatcgcgac agcgccagcc gtctggggat cagcatggcg
     3181 gacgtcgata acgccctgta caacgcgttt ggtcagcgac tgatttccac tatttatact
     3241 caggctaacc agtatcgcgt ggtgctggag cacaataccg aaaatacccc aggcctcgcg
     3301 gcgctggata ccattcgcct gaccagcagc gacggtggcg tggtgccgtt aagctcaatt
     3361 gccaaaattg agcagcgttt tgcgccgctt tccatcaacc atctggatca gttcccggta
     3421 acaaccatct cctttaacgt gccggataac tattcgctgg gcgatgcggt gcaggcgatt
     3481 atggacaccg aaaagacgct gaatctgccg gtggatatca ccacgcaatt ccaaggcagc
     3541 accctcgcct tccagtcggc gctgggcagc actgtctggc tgattgtcgc ggcggtggtg
     3601 gcgatgtata tcgtgctcgg cattctgtac gagagcttta ttcacccgat caccattctc
     3661 tcgacgctac ccaccgcagg ggtcggcgcg ctgctggcat taatgattgc cggtagcgaa
     3721 ctggatgtga ttgcgattat cggcattatt ttgctgatcg gtattgtgaa gaagaacgcc
     3781 atcatgatga tcgacttcgc gctggctgct gagcgcgagc aaggcatgtc gccgcgcgat
     3841 gcaatctacc aggcttgtct gttgcgtttt cgtccgatcc tgatgaccac cctggcggct
     3901 ctgcttggcg cgctgccgct gatgttgagt accggggtcg gcgcggaact gcgtcgtccg
     3961 ttaggtatcg gcatggtcgg cggtctgatt gtcagccagg tgctgacgct gttcaccacg
     4021 ccggtgattt atctgctgtt cgaccgcctg gtattgtgga ccaaaagtcg ctttgctcgc
     4081 catgaagagg aggcgtaagt gaagtttttt gccctcttca tttaccgccc ggtggcgacg
     4141 attttactgt cggtcgccat taccctgtgc ggcatactgg gtttccgtat gctgccggtc
     4201 gccccgctgc cgcaggtcga ttttccggtg attatggtca gcgcctcgct gcccggtgcg
     4261 tcgccggaga caatggcgtc ttccgttgcc acgccgctgg agcgctcact tgggcgcatt
     4321 gccggggtta gtgaaatgac ctccagcagt tcgctcggca gcacgcgcat tattttgcag
     4381 tttgattttg accgggatat caacggcgca gcgcgtgatg tgcaggcggc gatcaacgct
     4441 gcacaaagtt tgctgcccag cgggatgccc agccgcccga cctatcgcaa agcgaacccg
     4501 tcggatgcgc caattatgat cctcacgctg acatccgata cttattcgca gggtgaactg
     4561 tacgatttcg cctcgacgca gctggctccg acgatttcgc aaatcgacgg tgtcggtgat
     4621 gtcgatgtcg gtggcagctc gctgcccgcc gtgcgcgtcg ggctgaaccc gcaggcgctg
     4681 tttaatcagg gcgtgtcgct ggacgacgta cgcactgcca tcagcaatgc caacgtgcgt
     4741 aaaccgcagg gggcgctgga agatggcact caccgctggc agatccagac caacgatgaa
     4801 ctgaaaaccg ccgctgaata tcagccgctg attattcact acaacaacgg cggcgcggtt
     4861 cgtctgggcg atgtggcgac ggtgactgac tcagtgcagg atgtgcgcaa cgccgggatg
     4921 accaacgcca aaccggctat tttactgatg atccgcaaac tgccggaagc caatattatc
     4981 cagacggtag acagcatccg ggcaaaatta ccagagttgc aggagaccat tccggcggcg
     5041 attgatctgc aaattgccca ggatcgctcc cccaccattc gcgcctcgct ggaagaagtc
     5101 gagcaaacgc tgattatctc ggtggcgctg gtgattctgg tggtcttttt attcctgcgc
     5161 tcgggtcgcg ccactattat tcccgccgtt gcggtgccgg tttcgctgat tggtacgttt
     5221 gcggcgatgt acctgtgcgg attcagtctc aataatcttt cgctgatggc gctcaccatt
     5281 gctactggtt tcgtggtgga tgacgccatt gtggtgctgg aaaacattgc ccgtcatctg
     5341 gaagcgggga tgaaaccact acaggccgcg ctgcaaggta ctcgcgaagt cggctttacg
     5401 gtgctgtcga tgagtctgtc actggtggcg gtgttcctgc cgctgctgtt gatgggcgga
     5461 ttgccgggcc gactgttacg cgagtttgcc gtgacgcttt ctgtcgccat tggtatttcg
     5521 ttgctggttt ccctgacgct aacgccaatg atgtgcggct ggatgctaaa agccagcaag
     5581 ccgcgtgagc aaaagcgact gcgtggtttt ggccgcatgt tggtagccct gcaacaaggc
     5641 tacggcaagt cgctgaaatg ggtgctcaat catacccgtc tggtgggcgt ggtgctgctt
     5701 ggcaccattg cgctgaatat ctcgatcccg aaaaccttct tcccggagca ggacaccggc
     5761 gtactgatgg gtgggataca ggcggatcag agtatttcgt ttcaggcgat gcgcggcaaa
     5821 ttgcaggatt tcatgaaaat tatccgtgac gatccggcag tggataatgt caccggcttt
     5881 accggcggtt cacgagttaa cagtgggatg atgtttatca ccctcaagcc acgcgacgaa
     5941 cgcagcgaaa cggcgcagca aattatcgac cgtctgcgcg tgaagctggc gaaagaaccg
     6001 ggggcgaatc tgttcctgat ggcggtacaa gatattcgcg ttggtgggcg tcagtcgaac
     6061 gccagctacc agtacacgtt gttatccgac gacctggcgg cactgcgcga atgggagccg
     6121 aaaatccgca aaaaactggc gacgttgccg gaactggcgg acgtgaactc cgatcagcag
     6181 gataacggcg cggagatgaa tctggtttac gaccgcgaca ccatggcacg gctgggaatc
     6241 gacgtacagg ccgccaacag tctgttaaat aacgccttcg gtcagcggca aatctcgacc
     6301 atttaccagc cgatgaacca gtataaagtg gtgatggaag tggatccgcg ctatacccag
     6361 gacatcagtg cgctggaaaa aatgttcgtt atcaataacg aaggcaaagc gatcccgctg
     6421 tcgtatttcg ctaaatggca accggcgaat gccccactat cggtgaatca tcagggatta
     6481 tcggcggcct cgaccatctc gttcaacctg ccgaccggaa aatcgctctc ggacgccagt
     6541 gcggcgatcg atcgcgcaat gacccagctt ggtgtgcctt cgacggtgcg cggcagtttt
     6601 gccggcacgg cgcaggtttt ccaggagacg atgaactcgc aggtgatcct gattatcgcc
     6661 gccatcgcca cggtgtatat cgtgctgggt atcctttacg agagttacgt acatccgctg
     6721 acgattctct ccaccctgcc ctcggcgggc gttggagcgc tgttggcgct ggagctgttc
     6781 aatgccccgt tcagcctaat cgccctgata gggatcatgc tattaatcgg catcgtgaag
     6841 aaaaacgcca ttatgatggt cgattttgcg cttgaagccc aacggcacgg taacctgacg
     6901 ccgcaggaag ctattttcca ggcctgtctg ctgcgttttc gcccgattat gatgaccacc
     6961 ctggcggcgc tgtttggcgc gctgccgctg gtattgtcgg gcggtgacgg ctcggagctg
     7021 cggcaacccc tgaggatcac cattgtcggc ggtctggtga tgagccagct cctgacgctg
     7081 tataccacgc cggtggtgta tctctttttc gaccgtctgc ggctgcgttt ttcgcgtaaa
     7141 cctaaacaaa cggtaaccga gtaaatgaca gatcttcccg acagcacccg ctggcaatta
     7201 tggattgtgg ctttcggctt ctttatgcag tcgctggaca ccaccatcgt aaacaccgcc
     7261 cttccctcaa tggcgcaaag cctcggggaa agtccgttgc atatgcacat ggtcattgtc
     7321 tcttatgtgc tgaccgtggc ggtgatgctg cccgccagcg gctggctggc ggacaaagtc
     7381 ggcgtgcgca atattttctt taccgccatc gtgctgttta ctctcggttc actgttttgc
     7441 gcgctttccg gcacgctgaa cgaactgttg ctggcacgcg cgttacaggg cgttggcggt
     7501 gcgatgatgg tgccggtcgg caggttgacg gtgatgaaaa tcgtaccgcg cgagcaatat
     7561 atggcggcga tgacctttgt cacgttaccc ggtcaggtcg gtccgctgct cggtccggcg
     7621 ctcggcggtc tgctggtaga atacgcgtcg tggcactgga tctttttgat caacattccg
     7681 gtggggatta tcggtgcgat cgccacattg atgttaatgc cgaactacac catgcagacg
     7741 cggcgctttg atctttccgg ctttttattg ctggcggttg gcatggcggt attaaccctg
     7801 gcgctggacg gcagtaaagg tacaggttta tcgccgctgg cgattgctgg cctggtcgca
     7861 gttggcgtgg tggcactggt gctttatctg ctgcacgcca gaaataacaa ccgcgccctg
     7921 ttcagtctga aactgttccg cactcgtacc ttttcgctgg gcctggcggg gagctttgcc
     7981 ggacgtattg gcagtggcat gttgcccttt atgacaccgg ttttcctgca aattggcctc
     8041 ggtttctcgc cgtttcatgc cggactgatg atgatcccga tggtgcttgg cagcatggga
     8101 atgaagcgaa ttgtggtaca ggtggtgaat cgctttggtt atcgtcgggt actggtagcg
     8161 accacgctgg gtctgtcgct ggtcaccctg ttgtttatga ctaccgctct gttgggctgg
     8221 tactacgttt tgccgttcgt cctgttttta caagggatgg tcaactcgac ccgtttctcc
     8281 tccatgaaca ccctgacgct gaaagatctc ccggacaatc tggcgagcag cggaaacagc
     8341 ctgctgtcga tgattatgca attgtcgatg agtatcggcg tcaccatcgc cgggctgttg
     8401 ctggggcttt ttggttcgca gcatgtcagc gtcgacagcg gcaccacaca aaccgtcttt
     8461 atgtacacct ggcttagcat ggcgtttatc atcgcccttc cggcgttcat ctttgccaga
     8521 gtgccgaacg atacgcatca aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga
     8581 agttctggcg acccggtatt accggcaaac tgtttctggc gattttcgcc acctgcattg
     8641 tcttgctgat cagtatgcac tgggcggtgc gtatcagttt tgagcgtggc tttattgatt
     8701 acatcaaaca cggcaatgaa cagcgattac agctgttaag tgatgcgctt ggcgagcagt
     8761 atgcgcagca tggtaactgg cgcttcctgc gcaacaacga tcgctttgtc tttcagatcc
     8821 tgcgttcatt tgaacacgat aattcggaag ataaacccgg cccgggtatg ccgccgcacg
     8881 gctggcgtac ccagttctgg gtggtcgatc aaaacaacaa agtactggtt ggtccgcgag
     8941 cgccgattcc acctgacggt acacggcgac ccattctggt caacggtgcg gaagtgggcg
     9001 cggtgattgc ctcccctgtt gagcggctga cccgcaatac tgatatcaat ttcgataaac
     9061 aacagcggca aaccagctgg ctgatcgtcg ccctggcaac gttactcgcg gcgctcgcca
     9121 cctttctgct ggcgcgcggt ttgctggcac cggtaaaacg acttatcgat ggcacgcaca
     9181 aactggcggc gggcgatttc actacccgcg taacgcccac cagtgaagat gaactgggca
     9241 aactggcgca agacttcaac cagctcgcca gcacgctgga gaaaaaccag caaatgcggc
     9301 gcgattttat ggccgatatt tctcacgaac tgcgtacgcc attagcggtg ctgcgcggtg
     9361 aactggaagc cattcaggat ggcgtgcgca aattcacgcc ggagacggtg gcgtctttac
     9421 aggcggaggt cggtacactg accaaactgg ttgacgatct tcatcagttg tcgatgtctg
     9481 atgaaggcgc tctcgcctat caaaaagcac cggtagattt gatcccactg ctggaagtgg
     9541 cgggcggcgc atttcgcgaa cgattcgcca gccgtggcct gaaactgcaa ttttccctgc
     9601 cagacagtat caccgtattt ggcgatcgcg accgtttaat gcagttattc aataacttac
     9661 tggaaaacag cctgcgctac actgacagcg gcggcagcct gaaaatctct gccgagcagc
     9721 acgacaaaac ggtgcgcctg acctttgccg acagtgcgcc aggtgtcagt gacgatcagc
     9781 tacaaaaatt gtttgaacgt ttttatcgca ccgaaggttc ccgcaaccgc gccagcggcg
     9841 gttccgggct ggggctggcg atttgcctga acattgttga agcccataat ggccgcatta
     9901 ttgccgccca ttcgcctttt ggcggggtaa gcattacagt agagttaccg ctggaacggg
     9961 atttacagag agaagtatga ccgagttacc aatcgacgaa aacacaccgc gtattttgat
    10021 cgtggaagat gaaccgaagc tggggcagtt gctcattgat tatctgcgtg ctgcgagcta
    10081 tgcgccgacg cttatcagcc acggcgatca ggtactggcg tatgtgcgcc agacaccacc
    10141 ggatctgatc ctgttggatc tgatgctccc tggcaccgat ggcctgacat tatgccggga
    10201 aattcgtcgt ttttccgatg ttccgatcgt gatggtgacg gcaaaaatcg aagagatcga
    10261 ccgcctgctg gggctggaga ttggcgccga tgactacatc tgcaagccgt acagcccacg
    10321 ggaagtggta gcgcgtgtca aaaccatttt gcgccgttgt aaaccgcaac gcgaacttca
    10381 gcaacaggat gccgaaagcc cgttgattat cgacgaaggt cgttttcagg cttcatggcg
    10441 cggtaaaatg cttgacctga cgcctgcgga atttcgtctg cttaaaacgc tctctcacga
    10501 accaggaaaa gtgttctccc gcgagcaatt gctcaatcat ctttatgacg actaccgcgt
    10561 agtaaccgac cgcaccattg acagccacat taaaaatctg cgccgcaagc tggaatctct
    10621 cgacgccgaa cagtcattta tccgcgccgt ttacggcgtt ggttaccgct gggaagccga
    10681 cgcctgccgc atcgtttagt tttagcgaca ttactttgtt agccggagat ggcgttcaga
    10741 cccgatccgg caacattatt ccacgcatgg tcagcaaact gacattatct ctccgccctg
    10801 gcaggcatca gaaaatcgct catactttaa tcagtaaaca gcacctttag atgctgtttt
    10861 cgataagcaa tttcaatcaa ggagtcatta tggctggttg gtttgaactc agtaagagca
    10921 gtgataatca gttccggttt gtgctaaaag cgggcaatgg tgagactatc ctcaccagcg
    10981 agctgtatac ctcaaaagcc tctgcggaaa agggcatcgc gtcggtgcgt agcaacagcc
    11041 cgcaagaaga gcgctatgag aaaaaaacgg caagcaacgg caaattctat ttcaatctga
    11101 aagccgctaa tcatcaaatt atcggctcca gccagatgta cgccaccgcg caatctcgtg
    11161 aaaccggaat tgcctccgtt aaagccaatg gcacaagcca gacggtgaaa gacaatacgt
    11221 aattacgatg ccgggcgtgg gttgttggcg tccggcgctt taccagatcc cccctacagc
    11281 gctacaatgc ccgcccttaa agtgggggcc tcccctaacc gcttcatcag gtgaagcgga
    11341 tctgacctgt catcagaacg agagaattat gtttaaaccg gaactccttt ccccggcggg
    11401 aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca gatgctgttt atgcgggcca
    11461 gccgcgttac tccctacgtg tgcgcaacaa cgaattcaac cacgaaaatc ttcagctcgg
    11521 catcaatgaa gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca ttgcaccaca
    11581 caacgccaag ctgaaaacct ttatccgtga cctgaaaccg gtggtggaaa tggggccgga
    11641 tgcgctgatt atgtccgatc cagggctgat tatgctggtg cgtgagcact tcccagaaat
    11701 gccgatccac ctctcggtgc aggctaacgc cgtgaactgg gcgacggtga aattctggca
    11761 gcaaatggga ctgacccgcg tgatcctctc tcgcgagctg tcgctggaag agattgaaga
    11821 gatccataat caggtgccgg atatggagat cgagatcttc gttcacggcg cactgtgcat
    11881 ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac aagcgcgacc cgaaccaggg
    11941 cacctgcacc aacgcctgcc gctgggagta caacgtccag gaagggaaag aagatgacgt
    12001 tggcaacatc gtacacaagt acgagccgat tccggtgcaa aatgttgagc cgacgctggg
    12061 tatcggcgcg ccaaccgaca aagtgtttat gatcgaagaa gcccagcgtc cgggcgagta
    12121 tatgaccgcg tttgaagatg agcacggcac ttacatcatg aactcgaaag atctgcgcgc
    12181 catcgcccat gtagaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa tcgaaggtcg
    12241 taccaaatct ttctactatt gcgcacgcac cgcacaggtt taccgcaaag ctatcgatga
    12301 cgccgctgcg ggaaaaccgt tcgataccag cctgctggaa actctggaag gtctggcgca
    12361 tcgtggctat accgaaggtt tcctgcgtcg tcatactcac gacgattatc agaactacga
    12421 atacggttat tcagtttctg accgccagca gtttgttggt gagtttaccg gtgagcgcaa
    12481 gggggacctc gcagcggtag cggtgaaaaa taaattctcc gttggcgaca gtcttgagct
    12541 gatgacgccg caaggcaaca ttaactttac ccttgagcac atggaaaacg ccaaaggcga
    12601 agctatgccg gtcgcaccag gcgatggtta tactgtgtgg ctcccggtcc cgcaggatct
    12661 tgagctaaat tacgcgctgc taatgcgtaa tttctccggg gaaaccacgc gtaaccccca
    12721 cggtaagtga ttaatttcga ttatttttcc cggatggaaa attcttagaa accgatcaca
    12781 tacagctgca tttattaagg ttatcatccg tttcgctgaa aaacataatc cataaaatgc
    12841 tagctgtacc aggaaccacc tccttagcct gtgtaatctc ccttacacgg gcttattttt
    12901 tgtacataag ctgtaaagaa ggattgatcc ctgataaccg tcttccattg accacatcga
    12961 tagaatcccc cttcatagca caatgccttt cacgtaacgg tatcgtgctc gcccaggttc
    13021 cggctacgca cagccagaac gcgcatgttt gacgcttacc aaaaaatatt ctcactctcc
    13081 acatttgaat gtcagacgag cgacgccatg taatcctgca ccttctgtct tcaggtcaac
    13141 tatctgcatt tttttgccct gagtaacaca gaaatgggct gcatcatttt ttactatatt
    13201 ttctgcacca gatattctgc ccctggctaa agaagcttcg gcttcggtgt agtattggtt
    13261 atcgagttta cgctgaatat tacttttata tgcaagacca aatttaccga tacttgtttc
    13321 atcattatgc acagcacaac cagatataag aaaaatacta attaatgata tagcagctat
    13381 ctttttcatc tcaccttccc ccattaaata ccaacgacac tctctagtgt ttaaatataa
    13441 taatggcatg attattataa ttgaatagga ttataataaa tgttctgtac aacatttcct
    13501 acataagtag gaattacgga cattgaggcc cttcagggta actccatggg ggctttaata
    13561 tattatattg aagatgccac tgtttagttg aatattaggt atatgctctt ttttgaaatt
    13621 tataggtggc agccgttagt attcgctgtc cccattgcaa gctcctggtg gtaaccactg
    13681 aatcctccat acttgaactg actttttatc ctccgacttt catcctgttc tgactccacc
    13741 ttttgttttc tgctctacac tatctacaga ccaatcataa aggcacatac gatcatggca
    13801 gaatttcccg ccagcttact gattcttaat ggcaaaagta ctgacaatct acccttgcgc
    13861 gaagcaatta tgctgttgcg tgaggaagga atgacgatcc atgtgcgggt cacctgggag
    13921 aaaggtgatg ccgcacgatt tgtagaggag gcccggaagt tgggcgtcgc aacggtgatt
    13981 gccggtggtg gcgatggcac cattaatgaa gtttctacgg cgttgattca gtgtgagggg
    14041 gatgacatac ccgcgctggg aattttgcca ttaggaaccg ccaatgattt tgccaccagt
    14101 gtagggattc ctgaggcact ggataaggcg ctgaaactgg caattgccgg taatgccatt
    14161 gcgatagata tggcgcaggt caacaaacaa acctgtttta ttaatatggc gacaggcgga
    14221 tttgggacgc gtattaccac agaaacgccg gaaaaattaa aagccgcgct gggtggcgtc
    14281 tcttacatca ttcatggctt aatgcgcatg gatactctgc aaccggaccg ttgtgaaatc
    14341 cgcggtgaaa actttcactg gcaaggtgac gccctggtca ttggtattgg taacgggcgt
    14401 caggccggtg gcggtcaaca attgtgcccg aacgcgttaa ttaacgatgg cttgctgcaa
    14461 ctgcgcattt ttaccggcga tgaaattctt ccggctctcg tatcaacctt aaaatctgac
    14521 gaagataacc cgaatattat cgaaggcgct tcgtcgtggt ttgatataca agccccacac
    14581 gaaatcactt ttaatcttga tggcgaaccg ttgagtgggc aaaacttcca tattgaaata
    14641 cttccggcgg cgttgcgttg tcgattacca ccagattgtc cattattgcg ttaatcaaac
    14701 tattctctct tgaaccaggg agaatccgta atgccggatg cggtgtaaac accttatccg
    14761 gcatacagaa caatactaca ccgtaattaa ttttacccgt gtcttctgca atgccaattt
    14821 atccacctct gacacagcat catcggtaac aataatgtca attttttcga ttggcagtac
    14881 ctgattaaaa ccgcgacgat taaatttcga tgaatcgaga acggcaatca ctttatttgc
    14941 ggcggtgacc atcgccccgc taatcgaata accttcatta aaggtcgtaa taccattaac
    15001 cgcatcaata ccatcagcac cgacaaacat taaatcggca ttaatatcct gcaatgaacg
    15061 ctcggcaatg gaaccatgca tcgagcgcgt tttatggcgg accgtgccac cacagacgac
    15121 cagagtaatg tctttatttt cggaaagggc aaacgctgcc gggagactgt tggtaatcac
    15181 cgtgatattt ttagcggtca ttaatccttc ggcgataagc attgtggtac ttccgctgtc
    15241 gagaataacg gtcatccctt catggatcat tttgactgcc gcctgcgcta ttctgttttt
    15301 tggcgcgctg gcgagctgaa atcgctcttt aaacccgact tcctgggtct cggtttcact
    15361 attaccggac attattttcg ccgcaccgcc atgaaagcgc gtaaccacgc ctttttgttc
    15421 gagaaagcgc aaatcggcac ggattgtcgc ttccgaggca gcaaatactc ccgccagatc
    15481 ctgaacaagc acggttccct gttcattcac taattggatg atcttattcc ttcgctcgaa
    15541 tgaattcatc gtcatttacc catattcaat tgtggctagt gtaaacgaag ggggaccatt
    15601 ggtgaatgat ctgttttgca caataccggg ccagcatcac gctggcccgc ggtttcatgg
    15661 aatgagcaac actttgcccg gcatggcatt acgagcgatg tcacgcaccg cctgggtgaa
    15721 gctttcaaag cttccacggt gagcgattaa tggatccagg cttaacttac gttctgtcag
    15781 caaccggctc gccgtttccc actcctgccc cggccaaggg ctggaatagt tcatccaact
    15841 gccgataacc gtcagctctt tacgcaatat tttgccaaac gttgccgatg ttaaatgcag
    15901 atcctgatgc aacgtgccca ccagcgccag ttgggcatga ggaccggcaa tctctaccgc
    15961 cagttcgaca gtttgcggta cgccagccgt ctcgaggata agctgattaa agcgcagttc
    16021 gcgtaaaacg ctctgcattt gcggcgcgct catttcgcta ctgttaaatg tttgcatcgc
    16081 accgaaagat tttgccagtg ccagtttttc tgagctaatg tcgatcgccg tcacactctt
    16141 tgctcccagc gcgacagcgc actgaatagc cagcaggcca atggttccgg caccaataat
    16201 aataacgttt ttattctcac aaccttgcgc taaatgaaaa gcatgcaggc caacggtaat
    16261 cggctcaata aaagccccat cctcaatagg catatccgtg ggtagagcaa agacattttt
    16321 tcgcttaacg acaatatatt cagcaaatcc accatcacgc cgcgagccaa taaaatcata
    16381 ttttgcgcac tgggaataaa accctttcaa acactctgga caagtaaaac agggtaataa
    16441 cggcacacag gcaaccgcat cgccagggtg taaatcatca acaccggatc ccaccgcatc
    16501 aatatagccg ctaaattcat ggcctaacgt tattggataa taatgtgcac catttttaaa
    16561 tatcctgggt aaatcggaac cacataagcc cgagctggca atttttaccc gcacctcatc
    16621 ctgatgttta atttcaggaa tgacgctttt tgcaacgcgc acgataccat cagtatcatt
    16681 cactactgat ttcataaaaa ctcctgattg ttaaggggga taaccccccc ctcaggtaaa
    16741 aattattctg cgagaacggc tttctcttgt ttagtaaaac cacgcgctct acgccaggtc
    16801 atgaaaatac cggtcagat
//
LOCUS       DAJTRQ010000082        16759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000082 DAJTRQ010000000
VERSION     DAJTRQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            192..2039
                     /gene="ldtD"
                     /locus_tag="OO332_002724"
     CDS             192..2039
                     /gene="ldtD"
                     /locus_tag="OO332_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5943148.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2220..2768
                     /locus_tag="OO332_002725"
     CDS             2220..2768
                     /locus_tag="OO332_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5943149.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2795..3442
                     /gene="gloC"
                     /locus_tag="OO332_002726"
     CDS             2795..3442
                     /gene="gloC"
                     /locus_tag="OO332_002726"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5943150.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO332_002727"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO332_002727"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5943151.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO332_002728"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO332_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5943152.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="OO332_002729"
     CDS             complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="OO332_002729"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5943153.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO332_002730"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO332_002730"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5943154.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12383
                     /gene="pepN"
                     /locus_tag="OO332_002731"
     CDS             9768..12383
                     /gene="pepN"
                     /locus_tag="OO332_002731"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5943155.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12590..13357)
                     /gene="ssuB"
                     /locus_tag="OO332_002732"
     CDS             complement(12590..13357)
                     /gene="ssuB"
                     /locus_tag="OO332_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5943156.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13354..14001)
                     /locus_tag="OO332_002733"
     CDS             complement(13354..14001)
                     /locus_tag="OO332_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5943157.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14013..15158)
                     /gene="ssuD"
                     /locus_tag="OO332_002734"
     CDS             complement(14013..15158)
                     /gene="ssuD"
                     /locus_tag="OO332_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5943158.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15155..16114)
                     /gene="ssuA"
                     /locus_tag="OO332_002735"
     CDS             complement(15155..16114)
                     /gene="ssuA"
                     /locus_tag="OO332_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5943159.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16107..16682)
                     /gene="ssuE"
                     /locus_tag="OO332_002736"
     CDS             complement(16107..16682)
                     /gene="ssuE"
                     /locus_tag="OO332_002736"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5943160.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4187 a         4322 c         4292 g         3958 t
ORIGIN      
        1 ataacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac gtatcggcgt
       61 ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt tcgtgagtat
      121 tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat gataaaaaca
      181 gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca atcagtttgt
      241 gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct gaagtaatcc
      301 ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag gcgcaagcca
      361 cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa gcccgcacgc
      421 aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag cttcaactgt
      481 tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt aaagccttcc
      541 agcaacagct ggcagaggtg gcgattgctg gtttccagcc gcagtttaat aaatgggtag
      601 agttactgac cgatcctggt gttaacggga tggcacgtga cgtggtgctc tctgatgcga
      661 tgatgggcta tctccatttc attgcaaata ttccgatcaa aggcactcgc tggctatata
      721 gcagtaaacc ttatgcgctt tcaacgccgc cgctttcggt gattaaccaa tggcagctag
      781 cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag catccgcaat
      841 atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg tggccccaac
      901 tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg gcgttgcgcg
      961 aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta cctggcgatg
     1021 acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca gcagaaacta
     1081 agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt cgtgccgcct
     1141 acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga ttgggagcag
     1201 atggtgctat tggcccggca acgcgtgact ggttaaacgt aacgcccgcc cagcgtgctg
     1261 gcgtgttggc actcaacatc cagcgattgc gcttgctgcc aacagagctt tctaccggga
     1321 tcatggttaa cattccagcc tattcgctgc tctactatca gaacggcaat caggtgctgg
     1381 attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc agtgcactta
     1441 acaacgtagt ggtaaacccg ccgtggaacg taccaccaac tctggcacgc aaagatattc
     1501 tgccgaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg gtgatgcgcg
     1561 gctggaacag cagagaagcg attgacccat ggcaggttga ctggtctaca atcacggcct
     1621 cgaatttacc gtttcgcttc cagcaggctc caggcccacg gaactcgctg gggcgctata
     1681 aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac cacaatctgt
     1741 tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa gcttccgatc
     1801 tggcgaatat gctgttgcag gatgcaggat ggaatgacaa acgtatttct gatgcgctga
     1861 agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat ctctactacc
     1921 tgacggcctt tgttggtgca gatgatcgta cccagtatcg tacagatatt tacaattatg
     1981 atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta atcaggtaaa
     2041 tgaagtagtt cggggaaatc aattgtcgta ataattagca tgattggggg cgattctctg
     2101 cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg cggttaaggt
     2161 gcctcttgtg cgccagaagt gcatataaac gataacattg acctgtagac ttgattatca
     2221 tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt gcactcggtg
     2281 ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg cgcattttga
     2341 cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat ggcagaggct
     2401 atattcagga agaattggca aaacttaacc attttttccg cgattaccgc gcgaacaaaa
     2461 taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg ttgttaggca
     2521 cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac aatgaactac
     2581 gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag gcgatggatt
     2641 tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct atgcgcgcag
     2701 gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg ccagcacggc
     2761 actggtagta atcgcttaac gaaacagggg cagtatgaac tatcgtatta ttccggtcac
     2821 cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg ccgcactggt
     2881 cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg gcctgacact
     2941 gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggcgcagcgg cggaactggc
     3001 gcaacattac ggcgtgccgg tttttggccc ggaaaaagaa gatgagttct ggctgcaagg
     3061 cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagtcgctga cgccagatcg
     3121 ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg tgttacattg
     3181 ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc tgctgatttc
     3241 tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg gcgatcataa
     3301 tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg tgacatttat
     3361 tccgggtcac ggaccattat ctacacttgg ttatgagcgc ctgcacaatc ccttcctgca
     3421 agacgaaatg cccgtctggt aaaggcgcat aaaaaagtcc acttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 acgcttacag cactgccaca atcgattcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaacag catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcaga ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcacct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaat gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggagg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacgatttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gagaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcgttcag actgttaatc agtgcatcga
     4381 agtcaagggt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacaca cgcttaacgc
     4501 tggtattttt tgccaggaag tcggcagcca cgcgtagtgc gccagtgcca cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaattatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gcataaaaaa aacaggacca acgtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccctggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt ttttatgaca cctgccactg ccgtcaataa
     6181 gttctgtcaa taaaaattta cggaactatt gatgagagtt tggtgtcttt atgtgtctgc
     6241 aggcatcttt ccattcaaac taacgtttcg ctaccgtgaa agtgctacaa agataaagat
     6301 ttagtttcaa aaagaaaaaa tatgtaacca aaagtaaaaa ttaaggagct ttgtgaacac
     6361 cgtcatattt ccatagaaac gtgatgatat ttacagcaat ttttatctat ttatatgatt
     6421 tacttatatt taaattaact aaacggaaat tttgtttctg atggaaactt tatcgacctg
     6481 gcacaaaatc ctctttcaga catccagaat gccaaaaaat agtatgaaaa ttgtgctatc
     6541 agctaagaaa aaactaatcc gcaataagaa tgcgggttat tttttgtgcg taaatgttgc
     6601 aatgtaatat tccgtaacag gatgatcgtt atggcggggg tgcaaataga ttgcttgact
     6661 taacaaacag ataacttgac agaaaggata aaaaagagcc agcaggcgct ggctctttga
     6721 ggaagaaaat cagaagctgg cgttgcgcgg agtacgtggg aacggaatca catcacgcac
     6781 gttttgcacg ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg
     6841 cggaacagta ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc
     6901 catttccagc atacgctcgt ccagaacgtc cagacgttct tcacgctggg agccaccaat
     6961 gatctcaccg atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt
     7021 gaggcgcata tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt
     7081 aaagtgttct tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac
     7141 cgggttttca aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac
     7201 ctgtgcgaaa tcggcttcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg
     7261 ttcagcgaag aatttcatgt cgtctgcgcg ttcttcgaga accgctttga agacatattt
     7321 cagcatggct tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc
     7381 cagcatccag aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg
     7441 gccgaaggta taaattttgg ataatgcgca agcgtaggtt tcgccgttca actggccaga
     7501 tacggtcagg aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc
     7561 gttacgcggc aggttttcca gatccagcgt agaaacgcgg aacatttcgc ctgcaccttc
     7621 ggtatcagat gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa
     7681 gcgatgcagc gcctgcgcca gcgtatggcg aacgcgcgcg acggcaccaa tcaggtttgt
     7741 gcgcggacgc aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat
     7801 cgggtaagtg tctggatctt caacccaacc agcaacttca accttgctgg cctgaatttc
     7861 aaattgttgc ccctggcccg gcgatgccac gactttaccc gtcacaatga ccgagcagcc
     7921 ggtggtcaga cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg
     7981 tacaggatca aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc
     8041 tcggcgggta cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg
     8101 gagtacgtcg gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa
     8161 taaacacttg tccacccgta aatggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccggatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctccg cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga agagggcgca ctggtcatca
    10021 gtaatttgcc ggagcgtttt acgcttaaga tcattaatga aataagcccg gcggcgaata
    10081 cggcgctgga agggctttat cagtcaggcg atgcgctttg cacccagtgt gaagccgaag
    10141 gtttccgcca tattacgtat tatctcgacc gcccggacgt gctggcgcgt tttaccacca
    10201 aaattattgc cgataaaatc aaatatccct tcctgctttc caatggtaac cgcgttgcgc
    10261 aaggcgaact cgaaaacgga cgccattggg tacagtggca ggacccgttc ccgaaaccgt
    10321 gctacctgtt tgcgctggtg gcaggcgact ttgatgtact gcgcgatacc tttaccacgc
    10381 gttctggtcg cgaagtagca ctggagctgt acgtcgatcg cggcaacctt gatcgcgcgc
    10441 cgtgggcgat gacctcgctg aaaaactcca tgaaatggga tgaagaacgc tttggcctgg
    10501 agtatgacct cgacatctat atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg
    10561 agaataaggg tctgaatatc tttaactcca aatatgtgct ggcccgcacc gacaccgcca
    10621 ccgacaaaga ttacctcgat attgaacgcg ttatcggcca tgaatatttc cataactgga
    10681 ccggtaaccg agtgacctgc cgcgactggt tccagctcag cctgaaagaa ggtttaaccg
    10741 tcttccgcga tcaggaattc agctctgacc ttggttcccg cgcagttaac cgcatcaata
    10801 atgtgcgcac catgcgcgga ttgcagtttg cggaagacgc cagcccgatg gcgcacccaa
    10861 tccgcccgga catggtcatt gagatgaaca acttctacac cctgaccgtt tacgagaagg
    10921 gcgcggaagt gatccgtatg atccacaccc tgctgggcga agaaaacttc cagaaaggga
    10981 tgcaactcta tttcgagcgt catgatggta gcgcagcgac ctgtgacgac tttgtgcagg
    11041 cgatggaaga tgcgtcgaat gttgatctct cccatttccg ccgttggtac agccagtccg
    11101 gtacgccggt tgtgaccgtc aaagacgact acaatccgga aactgagcag tacaccctga
    11161 ccatcagcca gcgcacgcca gccacgccgg atcaggcaga aaaacagccg ctgcatattc
    11221 cgtttgccat cgaattgtat gacaacgaag gcaaagtgat cccgttgcag aaaggcggtc
    11281 atccggtgaa ttccgtgctg aacgtcaccc aggcggaaca gacctttgtt tttgataatg
    11341 tctacttcca gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg aaactggaat
    11401 ataagtggag cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat gatttctccc
    11461 gttgggatgc ggcgcaaagt ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc
    11521 agcaagggca gccgctgtct ctgccggtac atgtggctga cgctttccgc gcggtgctgc
    11581 tcgatgagaa gattgatccg gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg
    11641 aaatggctga actgttcgat atcatagacc cgattgctat tgccgaagta cgcgaagcac
    11701 tcactcgtac tctggcgact gaactggcgg atgagctgct ggctatttac aacgcgaatt
    11761 accagagcga gtaccgtgtt gagcatgaag atattgcaaa acgcactctg cgtaatgcct
    11821 gcctgcgctt ccttgctttt ggtgaaacgc atctggctga cgtgctggtg agcaagcaat
    11881 accatgaagc aaacaatatg accgatgcgc tggcagcgct ttctgcggcg gttgctgcac
    11941 agctgccttg ccgtgacgcg ctgatgcagg agtacgacga taagtggcat caggacggtc
    12001 tggtgatgga taaatggttt atcctgcaag ccaccagccc ggcggcgaat gtgctggaga
    12061 cggtgcgtgg tctgttgcag catcgctcat ttaccatgag caacccgaac cgtattcgtt
    12121 cgttgattgg tgcgtttgcg ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg
    12181 gttaccagtt cctggtggaa atgcttaccg acctcaacag ccgtaacccg caggtagctt
    12241 cacgtctgat tgaaccgctg attcgcctga aacgttatga tgccaaacgt caggagaaaa
    12301 tgcgcgcggc gctggaacag ttgaaagggc tggaaaatct ctctggcgat ctttacgaga
    12361 agataactaa agcactggct tgatactttt gagccaaaat gcctgatgcg acgcttgacg
    12421 cgtcttatca ggcctacaac tccgttgaaa ttgctggttt tgtaggctgg ataaggcgtt
    12481 cacgccgcat ccggcagccg tgcccaactg cctgatgcga tgcttgacgt gtcttatcag
    12541 gcctacaagt ccgttgaaaa tgctggtttt gtgcgccaca tccggcatat taaccatgac
    12601 gccgaatcag ttgttcagaa tgcccacgcc gcattacccg ttgcaatact tccgcttcca
    12661 gctccgctaa tcgcaccgac cctaatcgac gcggacgggg gatatccact gtcaaatcca
    12721 gactaatttt tccctcttca attaacaaca cccggtcagc cattgctacc gcttcgctca
    12781 catcatgcgt caccaacagt acggtaaagc cgtgctgctg ccaaagtgac acaatcaaat
    12841 cctgcatctc gagtcgcgtt aaggcgtcca gtgccccgag cggttcatca agcaacaata
    12901 aacccggtcg atgaatcaac gctcttgcca gcgccacacg ctgtttctgc ccgccagaaa
    12961 gtgcggcagg ccattccccg gcgcgattct ccagtcctac cgcagccaac gcccgacgcg
    13021 cggcatcgcg ccactgacct ttaaggccta acccaacgtt atcaatcacc gatttccacg
    13081 gcagcagacg cgcatcctga aacatcattc gcgtatcatc ctgaatttca gccagcggtg
    13141 tggtgcccgc taacacatcg cctgcggttg gcgtttccag accggccagc aggcgcagca
    13201 gggtactttt accaccaccg ctgcggccca ccaccgccac aaactgacct gccggaatat
    13261 gtaaatccag ttggttcagg acgatatttt ccgcgtaatg tttgcttact gcattgagca
    13321 acaatggtgt gccctggttc agacgagcag tattcatatc gtggcctcct tcaaatgata
    13381 agccgggttc cagctcaacc agaggcgctc taacagctgc gcgctgacgt cagcaagttt
    13441 gccgagcagg gcgtaaagaa taatagcgac taccaccacg tctgtttgca aaaactctcg
    13501 cgcattcatc gccagataac caatgccgga attggcagaa atggtttcgg caacaatcag
    13561 cgtcagccac atcaggccca acgcaaaacg cacgccgacc ataattgagg gcagggcatc
    13621 gggcaggatc acatggataa acagcggtat gccggataat ccatagctac gcgccatctc
    13681 caccagcccg cgatcgatat tacggatgcc atgccaggtg ttgatataaa tggggaacag
    13741 cgtaccgagc gccaccagaa agattttcgc ggattcatca atgccaaacc acaaaatcac
    13801 cagcgggatc agcgccagat gctgccacag ttcgccgctg gcggagagcg tccagaacgc
    13861 cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac agccagccaa ccgaggaggc
    13921 cagttgccac accgccacga tgcccaccgg taaaaaccag ggggcaacgc gcaataacca
    13981 cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt gcgcgacttt acgggggata
    14041 aaatcattcg ccaccgcttc gccttgcgga ttcagcggct gcggctgggg aatttccgga
    14101 atggcgacat ccagatgtgg gaacagcaac tcgccaaccc gatacgcttc ttccagatgc
    14161 gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg cgtattcgtt gattcgcgcg
    14221 gcgaccgtag gaccatcgcc caccagcgcc gttccggcac cgccgcgcac taagccaacg
    14281 cccgcccata aattggggct gatctccaga ttgtcgcgct tgccgttatg tagcgccgcc
    14341 attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg cttgtgcttt ggcgatagtc
    14401 tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc acgcttcgtc gttagtttca
    14461 cgaacaatca catgcagacg aataccgaaa cgaattttgc gcccatacgc ggcagctttc
    14521 gcccgcactt gttcgatttt ctctttaaca agttccggcg gttcgcccca ggtgaggtag
    14581 agatcaactt gttctgccgc cagctcctgg gcgacatctg acgatccgcc aaagtaaagc
    14641 ggaggataag gctgttgaat cggcgggaag agcagttttg ctccgcgcac atgaatatgt
    14701 ttaccgttga aattgacggt ttcccccagc aataagcgcc gccagacctg ggtaaattcc
    14761 gccgaggctt cgtagtgctc gctatgatca aggaacacgc cgtcgcctgc cagctcttgt
    14821 ggatcgctgc ctgtgaccag attaaacaac gcacgaccat ttgagagacg gtcaagcgtg
    14881 gcggcctggc gggcggcaac agtaggtgag gttacgctgg gacgcagggc gacaagaaac
    14941 ttcagccgct gcgtcaccgg gatcatcgac gctgcaacca gccacgcatc ttcacacgat
    15001 cgccccgttg ggatcagcac accggtatag cccagacgat ccgccgcttg cgcaatttgt
    15061 tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg ttcccagata gtgcccgtca
    15121 ccgtgggtcg gtaaaaacca gaacatattc agactcataa ttgttttcct tccagttgag
    15181 tgggctgcca gatgcgctgg cgaatatcga cttttttcgg caccagacgg ttttcataaa
    15241 acagatctgc cgtttgctgc tgtaaggcgg caacctcggc gttaaccggt ttgatggtgg
    15301 taggagggcg atgatctaag taagaggcaa tcaccggtgc cggtaagcca atcgtttttg
    15361 ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa cgcatcggcc tcactaaagg
    15421 ttgccagtac gccctgaata aaagcgccgt ttttttctgc atagggacga gctgccagat
    15481 aaaacgatcc agtttgattg agatcggtgc cgtctttcag cacccgcacg ccgccctgta
    15541 ataacgcagc ggagtagtag ggatcccaga tagcccaggc gtcaacgtta ccttgctgga
    15601 acgcggcgcg ggcaccggca ggcgtcaggt aagtgggctg gatatcggtg aatttaagtc
    15661 cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact ggaacctttc tgaaaggcaa
    15721 ctttgtgacc tttaagatcg gctacggttt tgatcgggct gttttctgcc accagaatca
    15781 cttcggcttt gggcttcggt ggctcgacgc ccacatatac caaatcagcc ccggcagcct
    15841 gggcaaagat tggcggaata tccccggtac tgccgagatc aatactgcca acgtttaacg
    15901 cttccaacat ttgcggaccc gcggggaact ccacccagga gatttttgat tgcggatagc
    15961 gtttttccag taactggtgg ctttttgcca gcaccatacc aatactgcct ttctgatagc
    16021 ctatacgtaa cgcttcaggc gaggattctg cagcaaccgc aaaagtagag acgctaagca
    16081 atcccgccag cgccagttta atgatgttac gcatgggcat tacctcgcag agacagaagg
    16141 tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga aagtttctag cgcggtatca
    16201 agatggggtt gcagatttgg cgtgaactgg ggtctgtgat ggtaatcaat taattgtgag
    16261 tcatcggcaa acacgccgtg caggatctcc tgagctttca gtgcgcttaa aactggttta
    16321 agggcataat cgaccgccag cagatgggcc acggtaccgc ccgtcgccag cggtagcacc
    16381 actttgcctt gcaaagcgcg ctctggcagc aggtcgagca gggttttcag cgcgccggaa
    16441 taggcggctt tatacacagg cgtggcgaca atcagcccat cagcctgttg cagctgttcg
    16501 gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa gcagatcttc cggggcgaag
    16561 ttttgcagat tccagtgata aacctctaca tctaggccat ttagtttttc ccgcgcatat
    16621 tccagcaagg agctggagcg agaaggaaag cgaggactac ccgccagggt gatgacacgc
    16681 atactctctc cttataacca attgttcttt tttttgttaa cattgataac aattcggtca
    16741 gtctgtcgga gagacaaga
//
LOCUS       DAJTRQ010000083        16672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000083 DAJTRQ010000000
VERSION     DAJTRQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(32..616)
                     /gene="kptA"
                     /locus_tag="OO332_002737"
     CDS             complement(32..616)
                     /gene="kptA"
                     /locus_tag="OO332_002737"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5943161.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            865..1548
                     /locus_tag="OO332_002738"
     CDS             865..1548
                     /locus_tag="OO332_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5943162.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1545..2006
                     /locus_tag="OO332_002739"
     CDS             1545..2006
                     /locus_tag="OO332_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5943163.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2019..3191
                     /gene="iadA"
                     /locus_tag="OO332_002740"
     CDS             2019..3191
                     /gene="iadA"
                     /locus_tag="OO332_002740"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5943164.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3256..4167
                     /gene="hypT"
                     /locus_tag="OO332_002741"
                     /gene_synonym="qseD"
     CDS             3256..4167
                     /gene="hypT"
                     /locus_tag="OO332_002741"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5943165.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4160..4552)
                     /gene="iraD"
                     /locus_tag="OO332_002742"
     CDS             complement(4160..4552)
                     /gene="iraD"
                     /locus_tag="OO332_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5943166.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4549..4632)
                     /gene="idlP"
                     /locus_tag="OO332_002743"
     CDS             complement(4549..4632)
                     /gene="idlP"
                     /locus_tag="OO332_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5943167.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5225..6055
                     /locus_tag="OO332_002744"
     CDS             5225..6055
                     /locus_tag="OO332_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5943168.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6196..6969)
                     /gene="uxuR"
                     /locus_tag="OO332_002745"
     CDS             complement(6196..6969)
                     /gene="uxuR"
                     /locus_tag="OO332_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5943169.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7184..8644)
                     /locus_tag="OO332_002746"
     CDS             complement(7184..8644)
                     /locus_tag="OO332_002746"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5943170.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8725..9909)
                     /gene="uxuA"
                     /locus_tag="OO332_002747"
     CDS             complement(8725..9909)
                     /gene="uxuA"
                     /locus_tag="OO332_002747"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5943171.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10249..11592
                     /gene="gntP"
                     /locus_tag="OO332_002748"
     CDS             10249..11592
                     /gene="gntP"
                     /locus_tag="OO332_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5943172.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11843..12745)
                     /gene="fimH"
                     /locus_tag="OO332_002749"
     CDS             complement(11843..12745)
                     /gene="fimH"
                     /locus_tag="OO332_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5943173.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12765..13268)
                     /gene="fimG"
                     /locus_tag="OO332_002750"
     CDS             complement(12765..13268)
                     /gene="fimG"
                     /locus_tag="OO332_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5943174.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13281..13800)
                     /gene="fimF"
                     /locus_tag="OO332_002751"
                     /pseudo
     CDS             complement(13281..13800)
                     /gene="fimF"
                     /locus_tag="OO332_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13810..16446)
                     /gene="fimD"
                     /locus_tag="OO332_002752"
     CDS             complement(13810..16446)
                     /gene="fimD"
                     /locus_tag="OO332_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5943175.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16513..>16672)
                     /locus_tag="OO332_002753"
     CDS             complement(16513..>16672)
                     /locus_tag="OO332_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HCR5943176.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     PKMTGVME"
BASE COUNT         4047 a         4289 c         4063 g         4273 t
ORIGIN      
        1 aggcctatat aacccctgca atatattgaa tttattgttt ttgtacccca gtcggcataa
       61 acgaccactc aagaaactca acagcaaccg ttgatgtcag ccagaccccg ttttccgctt
      121 gccagaaagg gatgcctcgt ttcgccattt cctgtgcttt gacggttaag acaaccggcg
      181 atccgtgtct tgctcccact ttgcgtgccg ttgcttcatc agcagagaga tgcacgtaat
      241 gacgatcccc tgcaatcagc ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac
      301 cgtggtagag aaactgtggc ggtgtttttt cggcaaagga aatagcgacc tgcgaagtcg
      361 aatgcccctg aaccgcgcgg atgcacctac catcactgga ataactaaaa cgctttttat
      421 cgctggttgc gaccacggta tccagcaatg cacgggttaa tcgcttgccc gctttttggg
      481 cacaaagtat gagcttattg atatccgccc atccttcacg gtccagtacg ataccaatcg
      541 cctccggttt gtgtcggagg acaaaactta aaaacttgct cgtatccgcc agctcttttt
      601 cgttgtactt tgccatcgcg ttttcgtccc tggattttct gtttttattt actaatgtca
      661 ttgtcactgg gtttatgaat tttctgcaac tcaatcctgc gcagaacctt ttccacacag
      721 cgtaattttt cccctatgca aaaaacgcat agccagaaac atacttgcat tttccccata
      781 cccacatggc tgataaggtt tttcagttcc aggaattttc ctggctcaag caacacacaa
      841 acacaaaaaa caatggggat tgttatgaca caacaagggg atgctgtagc cggggagctg
      901 gcgacagaaa aagtgggcat taaagggtat ctggcttttt ttctcaccat aatctttttt
      961 tccggtgttt tttccggaac agacagctgg tggcgcgttt ttgatttttc cgttctgaac
     1021 ggatcgtttg ggcaactgcc tggtgcgaat ggagccacca cctcatttcg cggggtgggt
     1081 ggcgcggggg ctaaagatgg ctttctcttt gcgctggagc tggcaccgtc agtcattctc
     1141 tctctgggga ttatttcaat taccgatggt cttggtggat tacgcgccgc gcagcagtta
     1201 atgacacctg tgctaaaacc gttgttgggc attccaggga tctgctcgct ggcactgatc
     1261 gctaacctgc aaaacacgga tgccgccgcc ggtatgacca aagagctggc gcaggaaggt
     1321 gaaattaccg aacgcgataa agtgattttc gccgcatatc aaaccagcgg tagcgccatc
     1381 atcaccaact acttctcttc cggcgtcgcg gtgtttgcct ttctgggcac gtccgtaatc
     1441 gtacctttag ccgtcatcct ggtgtttaaa tttgtcggag ccaacatttt gcgcgtctgg
     1501 cttaacttcg aagaacgtcg caacccaacg caaggagcgc aagcatgaca actcaggtcc
     1561 gcaaaaacgt catggatatg tttatcgatg gcgcgcgccg tggctttact atcgccacga
     1621 ccaacctgct gccaaacgtg gtgatggcgt ttgtcatcat tcaggcgctg aaaattaccg
     1681 gcctgctcga ctgggtgggg catatctgtg aacccgtaat ggcgctgtgg ggattacccg
     1741 gtgaagcggc aaccgtcctt ttagccgcat taatgagtat gggcggcgca gtcggtgttg
     1801 ccgccagtct ggcaaccgct gacgcattaa ccggccacga tgtcaccgtt ttactgcccg
     1861 caatgtattt aatgggcaac ccggtgcaaa atgtcggtcg ctgcctgggg acagccgaag
     1921 tgaatgccaa atattacccg catatcatta cggtctgcgt gattaacgca ttgctgtcga
     1981 tctgggttat gcagcttatt gtttaacaag gagttaccat gattgattat accgcagccg
     2041 gttttaccct gctgcaggga gcgcatttgt atgcgccgga agatcgggga atttgcgatg
     2101 tcctcgtcgc taacggcaaa attatcgccg ttgccagcaa tatcccttct gacattgtac
     2161 cgaactgcac ggttgtcgat ctcagcgggc agatcctctg cccaggtttt attgatcaac
     2221 acgtccattt gattggcggt ggcggcgaag caggtcccac gacgcgcacg ccggaagtgg
     2281 cgctaagtcg cctgacggaa gcgggcgtca cgtcagtggt tggtctgctg ggcaccgact
     2341 ctatctctcg ccacctggaa tccctgctcg ccaagacccg tgcgctcaat gaagaaggca
     2401 tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc ccgcactatt acgggttccg
     2461 tggaaaaaga cgtggcgatt atcgatcgtg tgattggcgt gaaatgcgcc atctctgatc
     2521 accgttctgc cgcaccggac gtttatcacc tggctaatat ggcggcagaa tcccgcgttg
     2581 gcggtttgct cggcggtaaa cctggcgtca ccctgttcca catgggcgac agtaaaaagg
     2641 cgttaaagcc tatttatgac ctgctggaaa actgcgatgt gccgatcagc aagctgctgc
     2701 cgacccacgt taaccgcaac gtaccgttgt ttgagcaggc gctggagttc gcgcgcaaag
     2761 gcggcaccat cgatatcacc agcagcattg acgaaccggt cgcccctgcc gaaggtattg
     2821 cccgcgccgt tcaggcgggt attctgctgg cacgcgtcac cctcagctcc gacggcaacg
     2881 gtagccagcc gttcttcgat gacgaaggga atttaaccca tatcggtgtt gcaggttttg
     2941 aaacgttgct ggaaaccgtg caggtgctgg tcaaagacta tgatttcagt atcagcgatg
     3001 ccctgcgccc gctcaccagt agcgtagccg gtttccttaa catgagcggg aaaggcgaaa
     3061 ttctgccagg caatgacgca gacttactgg tcatgacgcc agaattgcgc attgagcagg
     3121 tatacgctcg cggcaaactg atggtcaaag acggcaaagc ctgcgtgaaa ggaacgtttg
     3181 aaacggctta aacggttgtc agccgcccta atggacgtag aatgccccca agggcggctg
     3241 acagagtaaa acgtaatgga tgactgtggt gcggttttgc ataatattga aaccaaatgg
     3301 ctttatgatt ttctgactct ggaaaaatgc cgcaattttt cccaggcggc agtcagtcgc
     3361 aacgtctcgc aaccggcatt cagccgccgc atccgtgcac tggaacaggc gattggcgtt
     3421 gaattgttta accgccaggt gacgccactg caactctcag aacaagggaa aatcttccat
     3481 tcgcagatcc gccatctgtt gcaacagtta gaaagcaacc ttgcagagct gcgtggcggc
     3541 agcgattacg cgcaacgtaa aatcaagata gccgctgcac actctctttc cctcggtctg
     3601 ttaccgtcca ttatcagcca gatgccgccg ctctttactt gggcaattga agccattgat
     3661 gtcgatgaag cggtcgataa actgcgtgaa gggcaaagtg actgtatttt ttcctttcac
     3721 gacgaagatt tgctggaagc gccgtttgac cacattcgct tatttgaatc tcaattgttc
     3781 cctgtctgcg ccagtgacga acacggagaa gcacttttta acctcgcgca gccacacttt
     3841 ccgttactga attacagccg caactcctac atggggcgat tgattaatcg caccctgacg
     3901 cgccacagtg agttaagttt cagcgccttt tttgtctctt cgatgagcga gcttttaaag
     3961 caggttgccc tcgacggctg tgggattgcc tggctgccgg agtatgccat ccaacaagaa
     4021 attcgcagcg ggcagctcgt tgtgctgaat cgggacgaac aggtgatccc gattcaggcc
     4081 tacgcatacc ggatgaatac ccgcatgaat ccagttgccg aacgcttctg gcgtgaactg
     4141 cgcgagctgg agattgtgct tagctgacat tctcaagcgt cgcactgcgc caaccgccat
     4201 tatcccagac cagatcaaac gttagcggtt catcgtcgtg gtacagcatt atctcaaggc
     4261 tgatcgccag agtgtggcag tacgcttctt tcaaattaac ctgcaacgcc gcaatccgtg
     4321 gctcatgaaa taacagtgca gaacgcagct gacggcaata ccagctctgg agatgtttgt
     4381 cgctcgggtt atgcacttgc cactcgggca tgcctgaaac aagtaatgac ggtaatgccg
     4441 aatgaggaga attaaaaaga gtaaggagat cgctgcatac cgatttacgc agcaatgacg
     4501 ttttattgcc tgaaatttca ggtaaaacag cctgaagtga ttggcgcatc attttgcgca
     4561 ctcctgacgt ttagcaacat atgcggcttg ccctgaacac cgggcaccac tgtattgatg
     4621 ctcattttcc atagccattc ccttttgttc attacatcca tgtcagtctg agacaggcct
     4681 gaatcctgtc agcaaagtat aaaaccttac ttgcctatga atatctactg acttttcagg
     4741 aaaaaagtca tgatttgata aaaatcatca aactcaaaaa aaaaccgcta gtatattctg
     4801 aatattccga atagttacgt agatgaaaat acttgtcacc gctgctactt aattccggtg
     4861 ggaaattatt tatttccgca taatcagcca tgtattattt gaaacaatta gtttgtaaat
     4921 aatataaatt gataacatat caaatacatc aaaaatacca ttcacaggca ttattaatat
     4981 agacctcaat atagcaacat caaattccaa ttagtaataa gatatgcttt taaattgcat
     5041 gcaaataatt ttaaatgagc gttttttgat actttatagt gtttcctacc ctttaatatc
     5101 actgtgtaga gttgatatca ctacaacaga aacatgcatg tcatttgttt acttctcttt
     5161 ttactacaga aaaatttatc tatttttcta aacatcagtt aaatactcat aaaaggtaac
     5221 tattatgtct atgccattaa gcaatgccgc taataaccaa ttcaatacaa ataatcactt
     5281 ccttcatcat ccaaaagtcg acagtgagtt aacacgtcga tatgaatatg cacgcctgga
     5341 tacggaaaat atctatcttc tgcctcttgc tcgtggcaat aaccataatt acgatggaaa
     5401 aagcgttgtc gaaattagaa aacttaatat tggaaaacag tcctggccat ttaattatgt
     5461 gacagataca tgcagagaat tcaatggtat tacaacaaca ggacgtatgc tctatcgaaa
     5521 cttgaaaatc acctcagctt tagacgaaat atatggtggt atatgtaaaa aggcgcatgc
     5581 tgctacagag ttagcagaag gtttacggct aaatttattt atgaagagcc ctttcgatcc
     5641 tgttgaggac tatacagttc atgaaataac attaggccct ggttgcaatg ttcctggtta
     5701 tgccggaacc acgataggat atatttcaac ccttccagct tctcaggcaa aaagatggac
     5761 caatgagcag ccaagaattg atatctatat tgatcagata ataactgtct caggggtagc
     5821 aaattcttcc ggattcgccc ttgccgcatt acttaatgct aatattgaga tgggaaatga
     5881 cccaatcatt ggtatagaag cctatcctgg taccgcggag atccattcaa aaatgggata
     5941 taacgttatc cccggtgatg aagatgcgcc actcaaacgc atgactctgc aaccgtcatc
     6001 tcttccagaa ctgtttgaat taaaaaatgg tgaatggaat tatattggta aatagccact
     6061 gagaagaatg agtactaata ttcaggttat acagatattt aaacaaaacc ctgcaattta
     6121 caaagtaata ctcattgcga cttcttaatt taagaaagcg caaggaacgt ttacccttgc
     6181 gcttattata ataagtcagg cgtcgacttt atccagcggc catgaatcaa agagatagcc
     6241 gtcaaaataa atatcgtcaa cgttcgagaa ttccagcaga cgttgcttaa cgttttccag
     6301 atgctgccac attgccagct tcgccgctcg ggcatctttt ttgattaacg cggcgaggat
     6361 ctgtttgtga tcgcccaacc actctttgcg atacaggctg tcatccagat ggctgtgcaa
     6421 ctggatccac attggattgt tttcccgcca ctgccaggac tgacggaata actccaccag
     6481 catgctgtta tgcgttgctt cagcaatggc gagatggaac tgcatgtcac cgctttcgct
     6541 gctacccggc gcgctggaag ccagttcacg ctcttccagt tgcaatgcct gacgcatttt
     6601 gacgatatct tcgcgggtag cctgcagtgc ggcaaactcg gcgatgttac tttccagtaa
     6661 ctgacgcgcc tgtaacagct caaaaggacc cgcatcattg cagacgttgg catccggact
     6721 gtctgtgttg tggctgcctg agctatcaag cacatagata ccggcacccc ggcgcacttc
     6781 caccagccct ttgatctcca gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc
     6841 aagcatttct gcaatttcac gctccggcgg cagccgttcg ccaggattgt acggcgtctt
     6901 tatgatcaga tcgcggatca tcgccccgac ttcctggtaa ggtctttgca cagaggtgac
     6961 agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt
     7021 gaacgcctta tccggcctac aacgggcaat tttacggccc ccgttaatct caaactatac
     7081 cattagcact ggaagttggc acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa
     7141 tatcagtttg attgaccagt aagtctgtag ggtaatcagt tagttacagc gcagccacac
     7201 actcgcgcgc accgcgttcg cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt
     7261 cggcattctg cggcagatca tcggcaaaaa taccgctcag gcccagcagc gctttcacgc
     7321 ggtctgcgcc ctgatactgc gcgttaatct tctggaactc ggccagcatc gggtcgacca
     7381 cgtcaatggc attaccctgc tcatccacgc cctgggtgta acgcatccag ccagccacgc
     7441 ccagcgtcag gtgacgccag ctgccgccgt tttgcaggtg cagacgcacc gggtccagta
     7501 gacgctgcgg taacttctgg ctgccgtcca tcgcaatctg ccaggtacgg tgacgcagag
     7561 acgggttgct gaaacgctcg atcagcagcg tcgcataggc gttcaggtcc gtaccttccg
     7621 gcatcgacag cgttggcgct tgttcctgca tcatcagggc aaaggccgct ttgcgataat
     7681 ccgggttagt catggtgtcg gcaatggttt catagccgcc gaggtaaccg aggtacgcca
     7741 ggaaagagtg gctgccgttc agcatacgca gcttcatcat ttcgaacggc acaacgtctg
     7801 caacgaactg tgcgcccact ttatcccagt ccgggcgacc attaacgaag ttatcttcaa
     7861 tcacccactg acggaacggt tcgcaggcaa tggcgcatgg gtcgtaaaca cccagctggt
     7921 cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca accatggtgc
     7981 acgggaaggt gacgttctct tcaatccatg ccgctagttg cgggtcacgc gcctgagcca
     8041 gccccagtac cgcgaccttc gccacatgac cgttttcacg cacgttatcg caggacatca
     8101 ccgtaaatgc tttcagccct ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa
     8161 tcgcggactt cggcgcagtc gggttttcca gatcgtgctt gatcagcggg ttattgagat
     8221 ccagttgacc gcttgccgca tcagcgcagt agcctttttc cgtgaccgtt agagagacaa
     8281 tcgccgtttg cggacgcgcc atcgcgttga gaataccttc gcagccgtcg atttccggat
     8341 gcagcgcttc tttcatcgaa ccgataattt tcagctcggt gctttctgcg cctttttccg
     8401 ctacggtgta cagcagttgc tgttttttca ggttttcgat cagcatacgg tcgttgcctg
     8461 gcatcaggtt aacttcgcag atgccccagt cgctgtcggt gctttccagc agatgatggg
     8521 tatacaacgc ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt
     8581 ccagacgaga atgatcccat gacgggcggg caaccggcag attgctgtca acaatagtag
     8641 tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc aaggaagaag gaaccggcac
     8701 ggcgcgcagc catgccggtg ggtattaacg gctaaagaaa gcgcgctgga tcgccagttc
     8761 gacaccgcga acttcggcca ggcctttcag acgaccaatt gcggagtaac cagggttggt
     8821 tttcttcttc aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc
     8881 ttctttgcct tccgctttac gacggtgttc ttcttcaaca atcgctttca ccacttcgta
     8941 catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg
     9001 catggtggag cgcagatggg tgaagtaaat acgcggaccg aactgcttga tcatatcaac
     9061 cagatcgttg tcagcacgca cgccgtagga accggtgcac atggtgaaac cgttcgccat
     9121 gctgtttacg gtatcaacca tccactgcat atcttcaatg gtggaaacaa tgcgcggcaa
     9181 gccgaggatc gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc
     9241 agcaactgga ataatcgctt tcaggaagac ggcaaagttt tcgcgcagtt tggctttgtc
     9301 gatatctttg tacagctcca ggtgcttacg gaactggtcg agggtatacc cttcttccgc
     9361 gcccggcaga ccggcaatga tgttacgcgt cagacgcgct ttgtcttcgt cgctcatagt
     9421 ggcgaagcgt tcagctgcct gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg
     9481 acgtttcagg atatgcattt cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt
     9541 ggagccgtct ggcagcacgt attcgaggtc agtacgggtc cagtcgagca ccggcatgaa
     9601 gttgtagcac acggtgcgaa tgccacactg cgccaggttg cgcagagtct gctgatagtt
     9661 agcgatccac tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa ttggtacgct
     9721 ttctacgaca gaccacacca ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc
     9781 ttctacggac catacttcgc cgttcgggat atggtgcagc gcggtaacca cgccagttgc
     9841 gcccgcctga cggacatcag ctaaagaaac cggatcgttt gggccgtacc agcgccaggt
     9901 ctgttccatg atttttcctc tttcacatag tgggttccac agcaaagaga cgcggaacct
     9961 tggagttgtt taggtatccg gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg
    10021 agatggcgta taagttctac cttttggtaa gacatgtcaa cccgaactct gaaattggtt
    10081 aaccacatca caagaatttc actctcatgg ttgttgctgc aaattgcagc aaagggtaat
    10141 cgatggttat tgatcagagg atgtgacatt catcgcaaca atggttgacc aatttgcatg
    10201 acatgtcgcc caaaataaca acggttcaac caagtaaggt aaaacgttat gcatgtgctt
    10261 aacattctct gggtggtatt cggcattggc ctgatgctga tactgaattt gaagttcaaa
    10321 atcaattcaa tggtggcttt gttggtggcg gcgctgtccg tcgggatgct ggcaggcatg
    10381 gatttgatgt cgctgctgca caccatgaaa gcgggcttcg gcaacacgct gggagaactg
    10441 gctatcatcg tggtgttcgg tgcggtcatc ggtaaattga tggtcgactc cggcgcggct
    10501 caccagatag cacatacgct gctggcgcgt ctaggtctgc gctatgttca gctgtcggtg
    10561 attatcatcg gccttatttt cggtctggcg atgttctatg aagtggcctt tatcatgtta
    10621 gcgccgctgg ttattgttat tgccgccgaa gctaaaattc cgttcctgaa actggcgatc
    10681 ccggcagtag cagctgccac taccgcacat tcactgttcc caccgcagcc gggtccggtg
    10741 gcgctggtga atgcttatgg cgcggatatg gggatggttt atatctatgg cgtactggtg
    10801 acgatcccaa gtgtaatctg cgcaggtctg atcctgccga agttcctcgg caatcttgag
    10861 cgcccaacgc catcattcct gaaagcagat caaccggtag atatgaataa tctgccctct
    10921 ttcggcgttt cgattctggt gccgctgatc ccggcgatca ttatgatctc taccaccatc
    10981 gccaatatct ggctggtaaa agatacccct gcctgggaag tggttaactt tattggttcc
    11041 tcgccgattg cgatgtttat tgcgatggtg gttgcattcg tactctttgg caccgcgcgt
    11101 ggtcatgaca tgcagtgggt gatgaacgct tttgaaagcg cggtgaagag tattgcaatg
    11161 gtgattctga tcatcggtgc gggtggcgtg ctgaagcaga ccatcatcga caccggcatt
    11221 ggcgacacca tcggcatgtt gatgtcccac ggcaatatct cgccctacat tatggcatgg
    11281 ctgatcactg tgctaattcg tctggcgacg ggtcagggtg tcgtttcggc gatgaccgcc
    11341 gccgggatta tcagtgctgc aatccttgat ccagcaaccg gtcagctggt tggcgtgaat
    11401 ccggcgctgc tggtactggc gacggctgcg ggttccaaca ccctcaccca cattaatgat
    11461 gcatctttct ggttgttcaa aggttacttt gacctgtcag taaaagacac gttgaaaacc
    11521 tggggtctgc tggagctggt caactccgtg gttgggctga ttattgtgct gattattagc
    11581 atggtagcgt aaaagaacac cagagcctgc caatggcagg ctcagactga tgactaaagt
    11641 ctaaaaatgt gcccggacag tcccctcgcc cctccgggga gagggttagg gtgaggggaa
    11701 cagaccagta aagtgcgctt tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc
    11761 tacaaagggc taacgtgcag gttttgtagg tctgatagcg tagcgcctca ggtaccagca
    11821 ttagcaatgc gctgtaattt ttttattgat aaacaaaagt cacgccaata atcgattgca
    11881 cattccctgc agtcacctgc cctccggtac gtgcgtaatt tgccgttaat cccagactta
    11941 ccgccgaagt tcctactgct cctaacgata ccgtgttatt cgttggaata atcgtaccgt
    12001 tgcgcgtcaa ctgtacgccg acgccctgcg ctggtgaaaa cgacgcggta ttggtgaaaa
    12061 tcgagttgcc cgcatctgcg gttgtgccgg agaggtaata ccccaggttt tggcttttcg
    12121 cacaataaac ggtaagagga attggcactg aaccagggta gtccggcaga gtaacggtga
    12181 catcacgagc agaaacatca caaccgccag tgggcaccac cacatcatta ttggcgtaaa
    12241 tattccacac aaactgaaaa tcatcgctgt tatagttgtt ggtctgtcgc aaaataagca
    12301 cggcaattaa tgaaccagct ttaatcgcca ccccgcccgc actgctcaca ggcgtcaaat
    12361 aaagcgccac cggccacggc ttatccgttc tcgaattata aacaacccgc ggcgtttcgc
    12421 tggtggtcgg aaatggatag ctactgccac tatattttac ggtcccggaa aaattagata
    12481 acacgccgcc gtaagccgag cctcgttgca gtgtgacata gtctgtaatg gtttccggat
    12541 aatcgttatg gcaaaagatt tgcgtcgaaa gatctacgac caggttttgc cccacattca
    12601 cgacaggcgc aaggtttaca taaacattag cgctgccacc gccaataggg atagcggtac
    12661 cattggcggt tttacaggcg aatgaccagg catttaccga ccagcccatc agcagtacag
    12721 caaacagggt aatagctcgt ttcattacaa ttatctcttc gggttcagct gtaggtatag
    12781 gtgatgctaa tcactgcctg aatggttccc tgagtggctc cgccatttac cgtcaatgct
    12841 ctgacctgta acgggaagtg cgctgattgt gaggaatcat ccacctgaac tgttttggtt
    12901 gcgccagtat tcaatgtgtt gccactgtca tcctgtagct ctaactggat gttttgcgcg
    12961 gtcccctggt ttttataata tccggtgctg tcggctgccc cgctgaagct ggcagtgacc
    13021 ctcgacgttc ccaccggaca attagtcaac tcaagcgcaa catcatgcca ggccgatgcc
    13081 gccccggcag acataagact gaaagaataa agatcgccga gatctaccgt ggcattggtg
    13141 gtggaaaccg tacacggttt ggcgacgacc ttaccgttca ccgtgatggt gacatcggct
    13201 gcctgtatcg tcgcacttgc gagcgccaac attgccgcca atacataccc acgtttgcac
    13261 catttcatga gcatctccag ttactgatat tcaagagtga aggtagccgt agcattgata
    13321 tgccccgcag tgacaggcac ctgtgtcgcc attagccggg cgtaaaaatt cagcgtattt
    13381 ggtttacccg gcgtcagggt cgtccacgaa atcgcagacg atggagcatt aaggggtatt
    13441 tgattttgct gctcattcag aagctgtatt cccagtcccg cagccgctga caccgtattt
    13501 tcaagtgcaa gcaggttggc attgtggcta tctgcaacgc cggtaaaccc aacttttacg
    13561 gcagaaacag ggtgacagca aaatacgaaa tggaacgaca ggagtcgtcg cgccaatgtt
    13621 gttaaattgc ttcgccgcgt tttccatcag atcaacagta aaattggttg attcagcggc
    13681 cacactacag ccgttatctc tgacatagcc gcggatagta atcgtgctat ccgcagccaa
    13741 aacgcgactt accgccagcc acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat
    13801 cacgccccct taacgacatt cagctgatag ctgggttaat aactgctgct gactctctgg
    13861 tggcagttga taattggcga cacagtgagc attttcctct tctccccatt tcacctgaac
    13921 ttttcccgct aaaggcattc cgctgaggta aaactgacca ttatccgcaa caatgccgct
    13981 actctggcta ctctctgatg tcaccatcgc cccaaacggc agcggcttat tattgtgagt
    14041 tagcgtcatg agcagtttta tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc
    14101 acgagtggga acaacgttag cgaccgcgtt atctaaatcg acgttatcag ccaaggtatt
    14161 ggtatccagc gccactctat tttcccgata ttcagtggca taaggcagca cggcataacc
    14221 acgccagtcg gtacgcaccc ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc
    14281 tttaacaagc accaccgtat cgtttaacgg ctgccccagc gttacgccat tggcatgagc
    14341 caatacccca ccgctgactc cgtaatagag ctgcttaata tcatcgctat ggctgtaacc
    14401 gatattggca ttgccgtaac caccgcgata attcagcgtg gcgtagcctg tacttccgct
    14461 attaccatcg cctcccccgg catagccggt ttgcacgcta taactgaggt tgttgtcttc
    14521 cagcaacgta ccgtatacac cagccagatt ggtcatccga ccgttgagat cgtgtgacat
    14581 gctgtagctg gcactggcat gtcgccactg agatttactg tcagaacgca gccagtggct
    14641 gaaaggaata ttgacgttaa gcgctaacat ctgatcccgt cctttttgcc aggcgttttt
    14701 cgtcaggcta tagctgagcg tccagttgat atcttcgaac gcagtattta atccagcctg
    14761 gaattgctca tcgacattac tcgttcccca ataagtttga tggctaccac tcaaatacag
    14821 tgttgatgtg cgcccgagtt gctgagtaac ggtgagctgt aatttcccgc gtttgttata
    14881 agcgaggttg taatagtcgg tgaatttcgg cttaacctga ataactccat cctgtgtttc
    14941 gatgttgtag ccattcattc gactgtatgt tgtatcagcg aaattaaaat atccgctggt
    15001 cgaataacgg taacccacta actgaatatt cgtgcctgat tcattgagcg atttgttata
    15061 gagaaaacgc accgattgtc cgtcatgctg actgtcatcg ggaagtgtgg aattagcctg
    15121 cgtcatatcc acagacagag cgcccagtgc ctccatgttt ttcccgatac cgaaattaaa
    15181 agcacgataa cgatccgcca gttgcgttcc accatatatt gtccagccag ccggaaggcc
    15241 gtggagtaat gtactctgga aaaagcgggg tttttcctgc tgcgcatttc cactacggta
    15301 ttctcctgcc ataatggaat aacgagtatg cccttcacgt tgcaaaagcg ggactgacga
    15361 atagggtacg gtaaaaatct gcgtgctgcc gtcagcctct ttgatcgtta cctgcaagtc
    15421 accactatta cctgcggcat agatatcgtt gatggtaaaa ggccccggtg gcaccgtact
    15481 attataaatg ccatatccat tttgtttaat agtgacctgt gcagtaccat gagcaatacc
    15541 gtggatcacc ggggcaaatc ctctttggct atcgggtaac atattgtcat ctgaggccaa
    15601 ttgtgcgccg cgaaagttaa taccatcgaa aatatcgccc tgggtataac catcacccaa
    15661 cgtcagccgg gaacgtaaag gaattatgtc tcgctcaatc caggtaatga tatgctgcca
    15721 tttatttttg ctacctgatg aactgtcgct actgttataa ctccaggtgg tattgtcgcg
    15781 taaacgccac gcaccaatat ttaacccact ctgtaggttt aaatatgcat aatggctgtt
    15841 acccccaatc cgattctgta cactatttcc gctgaaatta taattgagca atccggcatt
    15901 aataccggga tcccataact caggaggaat ataaccacgc gcgcgattac tcataaatgc
    15961 ctgagggatc gtcaggttca gtcgctgctg accaacatcc agatgcgcag tagcgtcctg
    16021 gaccattgtg gttaatggca cacaggcatc atccgccagc agattcatac cggcgacaga
    16081 agccgtattc agccccatac tggcgagttg cgcgcgtgtc aggcagggaa caatcccttg
    16141 ttcactgtcg cccgtattaa atgtgacatc acgcgttgcc atataaccat tattcaaata
    16201 gatatcgacg cgatacgtcc ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc
    16261 ggccacagcc tggggatcat ccgctaaaaa gcgcagatta aaatagaggt cggcagatga
    16321 caaaggtgcc tgtgcggcaa aagcacaggc gacaacgagt cggacaaaaa aaccagccaa
    16381 acgatgctta cgaatatgca agcattgtgt gtttcgctgg taaagtctta aattcagata
    16441 tgacattatt cctccggttt aaaatcaccc ggcagtcaat tctttttatt caggcgaaaa
    16501 attccccctg cgttattcca ttacgcccgt cattttgggg gtaagtgcgc cataatcatt
    16561 tattgttcgg taagtaatat tgcttcctgc atcagaaggc aatttaaccg tgctttcgcc
    16621 cattggaggc accaatgcat tttcaagaac ccgggttccg gcattcaact ct
//
LOCUS       DAJTRQ010000084        16663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000084 DAJTRQ010000000
VERSION     DAJTRQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..2071
                     /gene="pbpC"
                     /locus_tag="OO332_002754"
                     /pseudo
     CDS             1..2071
                     /gene="pbpC"
                     /locus_tag="OO332_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2221..2652
                     /gene="ndk"
                     /locus_tag="OO332_002755"
     CDS             2221..2652
                     /gene="ndk"
                     /locus_tag="OO332_002755"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5943177.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2802..3956
                     /gene="trmG/rlmN"
                     /locus_tag="OO332_002756"
     CDS             2802..3956
                     /gene="trmG/rlmN"
                     /locus_tag="OO332_002756"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5943178.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4241..5254
                     /gene="rodZ"
                     /locus_tag="OO332_002757"
     CDS             4241..5254
                     /gene="rodZ"
                     /locus_tag="OO332_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5943179.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5281..6399
                     /gene="ispG"
                     /locus_tag="OO332_002758"
                     /gene_synonym="gcpE"
     CDS             5281..6399
                     /gene="ispG"
                     /locus_tag="OO332_002758"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5943180.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6510..7784
                     /gene="hisS"
                     /locus_tag="OO332_002759"
     CDS             6510..7784
                     /gene="hisS"
                     /locus_tag="OO332_002759"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5943181.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7802..8422
                     /gene="yfgM"
                     /locus_tag="OO332_002760"
     CDS             7802..8422
                     /gene="yfgM"
                     /locus_tag="OO332_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5943182.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8433..9611
                     /gene="bamB"
                     /locus_tag="OO332_002761"
     CDS             8433..9611
                     /gene="bamB"
                     /locus_tag="OO332_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5943183.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9729..11201
                     /gene="der"
                     /locus_tag="OO332_002762"
     CDS             9729..11201
                     /gene="der"
                     /locus_tag="OO332_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5943184.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11271..11486
                     /locus_tag="OO332_002763"
     CDS             11271..11486
                     /locus_tag="OO332_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5943185.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11483..12853)
                     /gene="xseA"
                     /locus_tag="OO332_002764"
     CDS             complement(11483..12853)
                     /gene="xseA"
                     /locus_tag="OO332_002764"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5943186.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13015..14481
                     /gene="guaB"
                     /locus_tag="OO332_002765"
     CDS             13015..14481
                     /gene="guaB"
                     /locus_tag="OO332_002765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5943187.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14550..16127
                     /gene="guaA"
                     /locus_tag="OO332_002766"
     CDS             14550..16127
                     /gene="guaA"
                     /locus_tag="OO332_002766"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5943188.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16222..>16663)
                     /locus_tag="OO332_002767"
     CDS             complement(16222..>16663)
                     /locus_tag="OO332_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5943189.1"
                     /translation="QQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAATAA
                     ANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFIDQEL
                     KHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3841 a         4164 c         4665 g         3993 t
ORIGIN      
        1 tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc tcggtggcgc
       61 gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc acgctcacta
      121 tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa attctccagc
      181 tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg atcttgtatc
      241 ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt tgggcttatc
      301 tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg gtgttaccgc
      361 aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc gcgcgtaata
      421 aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta aaagagtcaa
      481 gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg ctgttttcgc
      541 gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat gccggtcttc
      601 aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc gcagctcact
      661 ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg gatcggttga
      721 tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc gatcgccagg
      781 atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga tccacccggc
      841 atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta actttgatag
      901 cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga acttacctgc
      961 tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac gcaatgttgg
     1021 attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc tcggcggcgc
     1081 tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc acggcaaggc
     1141 aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa tgtcgtcggg
     1201 ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc cggatagtgc
     1261 cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct atcgtgacgc
     1321 ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca gacctgacgg
     1381 cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga atcaggtcaa
     1441 taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga actcagtcac
     1501 tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg gtaactgtcg
     1561 ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt tactgtcaga
     1621 gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag ggcagcgcgt
     1681 cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc taccgctgga
     1741 accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct cgacaatttg
     1801 cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc gcgatggcgc
     1861 gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat caagtggagg
     1921 ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg ggcgcaacgt
     1981 gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg atgtggggca
     2041 aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg ttgctaaaac
     2101 tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg ttgctcatta
     2161 taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac agaggtaaaa
     2221 atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa aaacgtcatt
     2281 ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac caaaatgctg
     2341 cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa accgttcttt
     2401 gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct ggaaggtgaa
     2461 aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa cgcactggct
     2521 ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca cggttctgat
     2581 tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga agtgtgcccg
     2641 cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg cgccagaatt
     2701 tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt ttttcaaccc
     2761 tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa caattagtca
     2821 cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc aaccgtcagc
     2881 agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat caggtgatga
     2941 agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc aacaaagtgt
     3001 tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt gaagaacagc
     3061 gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc gtcgaaacgg
     3121 tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg gggtgtgcgc
     3181 tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg cgggtatcgg
     3241 aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa gtcaccggtc
     3301 agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc aacctgaaca
     3361 acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg tctaaacgtc
     3421 gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc gatatgatcg
     3481 acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac gaaattgtgc
     3541 cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt tatctggaga
     3601 aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac cacgttaacg
     3661 acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg tgtaagatca
     3721 acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg aacagccgta
     3781 tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg cgtaaaacgc
     3841 gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc gaccgtacga
     3901 aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg gtctgatagc
     3961 cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga agtattaagt
     4021 tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca gtatgtaacg
     4081 atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac agacggtctt
     4141 atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag catgatggtt
     4201 caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg aagccacgca
     4261 cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg aacaactagg
     4321 acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg tacgcgacat
     4381 tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat atatccgctc
     4441 ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg aaaagcaggc
     4501 tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta aacgccgcaa
     4561 aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg ttatcggcct
     4621 gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca ccactatggc
     4681 cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg ttccgttaaa
     4741 tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg tggatactac
     4801 cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg atccgcaaca
     4861 gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc cggtaccaac
     4921 ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg gcgtgaccac
     4981 gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct ggctggaggt
     5041 cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg gtaatttgaa
     5101 tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag tacagatcca
     5161 gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg ttgcgcgtct
     5221 gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg gagatttttc
     5281 atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt tgggaatgtg
     5341 ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg tacgacagat
     5401 gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga tatcgtccgt
     5461 gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca gcgggttaac
     5521 gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt agcggaatac
     5581 ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg tattcgcatg
     5641 gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa cgccggatcg
     5701 ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt gctggaatcc
     5761 gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa agtcagcgtg
     5821 aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa acagatcgat
     5881 cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc agtaaaatcc
     5941 gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg cgtatcgctg
     6001 gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc gctgcgtatc
     6061 cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga atttgatgtt
     6121 atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc gatggacgtt
     6181 tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac actcggcgtc
     6241 accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga ccgtctggac
     6301 aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag tcagctggac
     6361 gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg gaagcgcctc
     6421 gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag cgttgagggt
     6481 tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc attcgcggca
     6541 tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc acactgaaaa
     6601 acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag cagaccccgc
     6661 tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg tacacctttg
     6721 aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc tgtgtacgcg
     6781 ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat atcgggccga
     6841 tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag ttgggctgcg
     6901 aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc actgcccgct
     6961 ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct atcggttcgc
     7021 tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag cataaagaaa
     7081 agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg ctggattcaa
     7141 aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat tatctggacg
     7201 aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg gggatcgctt
     7261 acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc gttttcgagt
     7321 gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt tatgacggtc
     7381 ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg ggcctcgaac
     7441 gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct gttgtcgata
     7501 tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta gctgagcgtc
     7561 tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc aactttaaga
     7621 aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg ggtgagtctg
     7681 aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa acggcagttg
     7741 cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg agaaggacag
     7801 cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct tttttgctga
     7861 aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga ttggctggcg
     7921 ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct atcaaaatgc
     7981 ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa aatttgctgc
     8041 tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc aatttgttga
     8101 caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag acacgagcga
     8161 tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac agctcaagca
     8221 ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg ctgccattgt
     8281 tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg cgcgtagtgc
     8341 atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga tgcagatgaa
     8401 aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta ctgctgccag
     8461 gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa gaagatgtgg
     8521 taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg gcgtggagca
     8581 cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca ctggcggaca
     8641 acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg gatgatggca
     8701 aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa gagcctgcat
     8761 tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc gaaaaggcgc
     8821 aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca gtcgcgggtg
     8881 aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc agtaacggtc
     8941 agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac ctcgatatgc
     9001 cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg gctgtcgtgg
     9061 gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg atttggcagc
     9121 agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat gttgacacga
     9181 ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac ctgacggcgc
     9241 ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg aatgatttca
     9301 tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg gcgttgacca
     9361 ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg ctgacttctc
     9421 cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg cactggatta
     9481 acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt ttccagactg
     9541 aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc gtgtactcta
     9601 ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag gggccgtttt
     9661 cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat aaaaatgagg
     9721 ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga aaatccacgt
     9781 tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg ggtctgactc
     9841 gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt attgataccg
     9901 gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag tcgctgctgg
     9961 cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc ctgatgccgg
    10021 cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc ctggtggcaa
    10081 acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg cttggtttag
    10141 gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg ctggagcatg
    10201 tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa gacgctgaat
    10261 actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac gacttcgacc
    10321 cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt aagtctacac
    10381 tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct ggcacgacgc
    10441 gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc attgacaccg
    10501 ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc gtaatcaaaa
    10561 cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg cgcgaaggta
    10621 tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc tcacttgtca
    10681 ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg aaagaaacgc
    10741 tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct gccttgcacg
    10801 gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc tccacccgtc
    10861 gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat caccaaccgc
    10921 cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt tataacccgc
    10981 cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac aagcgctact
    11041 tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt attcagttca
    11101 aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc cagatgcgta
    11161 aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta agtattttat
    11221 ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat atggaactgc
    11281 attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt tgcccatcct
    11341 gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg ttgcaggtgt
    11401 taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg atatcgaaaa
    11461 aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt accggctgga
    11521 tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc atttcacccg
    11581 ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta acgctgtatc
    11641 cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca ttaccgaaac
    11701 gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat tccagttgct
    11761 gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc agccgctgtg
    11821 ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca aagctcatcc
    11881 gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc ggatgctgct
    11941 gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga taataatcca
    12001 tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct tgctgattac
    12061 ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca acaaaatcgg
    12121 caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg ctggcaaaaa
    12181 tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa ccgccgccgc
    12241 gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg gcgcgaacaa
    12301 tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc ggtagagaag
    12361 gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt tttgaggtga
    12421 tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga tcgaacaaac
    12481 cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc ccttcaccgg
    12541 ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag agcgtaatat
    12601 tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg cggttgctgt
    12661 tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag taccagtgac
    12721 cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt cccatctcat
    12781 gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt gcaggggatt
    12841 gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca gtcgatagta
    12901 acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca atcggttacg
    12961 ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt gcccatgcta
    13021 cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc tcattctacc
    13081 gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg tctgaatatc
    13141 cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat tgctctggct
    13201 caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca ggcagaagaa
    13261 gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac tgttctgcca
    13321 accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc gggctatccg
    13381 gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt gcgttttgtt
    13441 accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct ggtcaccgtg
    13501 cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg cgttgaaaaa
    13561 gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa agacttccag
    13621 aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg tgttggtgct
    13681 gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc cgctggcgtt
    13741 gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca gcgtatccgt
    13801 gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc aacagctgca
    13861 ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat cggccctggt
    13921 tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac tgccgttgct
    13981 gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg tggtattcgc
    14041 ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat ggtaggttcc
    14101 atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg ccgttcttat
    14161 aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc tgaccgttat
    14221 ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg tcgcgtagct
    14281 tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg ctcctgtatg
    14341 ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt tgtacgtatc
    14401 agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa agagtccccg
    14461 aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga tttatattat
    14521 ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag catcgcatcc
    14581 tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg cgtgagctgg
    14641 gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt gacttcaatc
    14701 caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac agtccgcgtg
    14761 cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat ggcatgcaga
    14821 ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa tttggctacg
    14881 cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat gcgctgaccg
    14941 cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt accgctatcc
    15001 cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt atggctaacg
    15061 aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc cgccagggta
    15121 tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg tggacgccag
    15181 cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac gataaagtca
    15241 tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg caccgtgcta
    15301 tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc aacgaagcag
    15361 agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac gtaccggcag
    15421 aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa cgtaaaatca
    15481 tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa gacgtgaagt
    15541 ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct gcaaccggta
    15601 aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag atgaagatgg
    15661 gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt ggtctggagc
    15721 tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc cttggcgttc
    15781 gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct gacgccatct
    15841 tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg ttcaccgtct
    15901 tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac tgggttgtct
    15961 ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat ctgccgtacg
    16021 atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt tcccgcgtgg
    16081 tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt tgatccctgc
    16141 actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca agaatcatag
    16201 gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct cttctgcttt
    16261 gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg cgtcagcttc
    16321 agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga taaattcgtt
    16381 ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct gaatctctaa
    16441 gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg cttcggcgtc
    16501 cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt tgttcgcggc
    16561 ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc gctccgcgac
    16621 ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgt
//
LOCUS       DAJTRQ010000085        16393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000085 DAJTRQ010000000
VERSION     DAJTRQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO332_002768"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO332_002768"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5943190.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO332_002769"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO332_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5943191.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="OO332_002770"
     CDS             2337..2552
                     /locus_tag="OO332_002770"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943192.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="OO332_002771"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="OO332_002771"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5943193.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="OO332_002772"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="OO332_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5943194.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="OO332_002773"
     CDS             5918..6415
                     /locus_tag="OO332_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5943195.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="OO332_002774"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="OO332_002774"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5943196.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="OO332_002775"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="OO332_002775"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5943197.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="OO332_002776"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="OO332_002776"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5943198.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="OO332_002777"
     CDS             9100..9663
                     /locus_tag="OO332_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5943199.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="OO332_002778"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="OO332_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5943200.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="OO332_002779"
     CDS             complement(10255..11235)
                     /locus_tag="OO332_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5943201.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="OO332_002780"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="OO332_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5943202.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="OO332_002781"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="OO332_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5943203.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="OO332_002782"
     CDS             12538..12828
                     /locus_tag="OO332_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5943204.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="OO332_002783"
     CDS             12836..13429
                     /locus_tag="OO332_002783"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5943205.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="OO332_002784"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="OO332_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5943206.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="OO332_002785"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="OO332_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..16353
                     /locus_tag="OO332_002786"
     CDS             15880..16353
                     /locus_tag="OO332_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5943207.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALGNAANLLI"
BASE COUNT         4052 a         4219 c         4126 g         3996 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttctaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaaaaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggtcgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg cctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaattga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtgga ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtctcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 agccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccga ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa caatcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cacgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgttcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aagcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattacc
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 gccttgggta atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
    16381 cgtggcttcc att
//
LOCUS       DAJTRQ010000086        15980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000086 DAJTRQ010000000
VERSION     DAJTRQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(18..350)
                     /gene="emrE"
                     /locus_tag="OO332_002787"
     CDS             complement(18..350)
                     /gene="emrE"
                     /locus_tag="OO332_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5943208.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(695..1009)
                     /gene="fliE"
                     /locus_tag="OO332_002788"
     CDS             complement(695..1009)
                     /gene="fliE"
                     /locus_tag="OO332_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5943209.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1223..2881
                     /gene="fliF"
                     /locus_tag="OO332_002789"
                     /pseudo
     CDS             1223..2881
                     /gene="fliF"
                     /locus_tag="OO332_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            2874..3869
                     /gene="fliG"
                     /locus_tag="OO332_002790"
     CDS             2874..3869
                     /gene="fliG"
                     /locus_tag="OO332_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5943210.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3862..4548
                     /gene="fliH"
                     /locus_tag="OO332_002791"
     CDS             3862..4548
                     /gene="fliH"
                     /locus_tag="OO332_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5943211.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4548..5921
                     /gene="fliI"
                     /locus_tag="OO332_002792"
     CDS             4548..5921
                     /gene="fliI"
                     /locus_tag="OO332_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5943212.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5940..6383
                     /gene="fliJ"
                     /locus_tag="OO332_002793"
     CDS             5940..6383
                     /gene="fliJ"
                     /locus_tag="OO332_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR5943213.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6380..7507
                     /gene="fliK"
                     /locus_tag="OO332_002794"
     CDS             6380..7507
                     /gene="fliK"
                     /locus_tag="OO332_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5943214.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7612..8076
                     /gene="fliL"
                     /locus_tag="OO332_002795"
     CDS             7612..8076
                     /gene="fliL"
                     /locus_tag="OO332_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5943215.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8081..9085
                     /gene="fliM"
                     /locus_tag="OO332_002796"
     CDS             8081..9085
                     /gene="fliM"
                     /locus_tag="OO332_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5943216.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9082..9495
                     /gene="fliN"
                     /locus_tag="OO332_002797"
     CDS             9082..9495
                     /gene="fliN"
                     /locus_tag="OO332_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5943217.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9498..9863
                     /gene="fliO"
                     /locus_tag="OO332_002798"
     CDS             9498..9863
                     /gene="fliO"
                     /locus_tag="OO332_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5943218.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            9863..10600
                     /gene="fliP"
                     /locus_tag="OO332_002799"
     CDS             9863..10600
                     /gene="fliP"
                     /locus_tag="OO332_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5943219.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10610..10879
                     /gene="fliQ"
                     /locus_tag="OO332_002800"
     CDS             10610..10879
                     /gene="fliQ"
                     /locus_tag="OO332_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5943220.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10888..11592
                     /locus_tag="OO332_002801"
     CDS             10888..11592
                     /locus_tag="OO332_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR5943221.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            11877..12500
                     /gene="rcsA"
                     /locus_tag="OO332_002802"
     CDS             11877..12500
                     /gene="rcsA"
                     /locus_tag="OO332_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5943222.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12544..12732)
                     /gene="dsrB"
                     /locus_tag="OO332_002803"
     CDS             complement(12544..12732)
                     /gene="dsrB"
                     /locus_tag="OO332_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5943223.1"
                     /translation="MKVNDRVTVKTDSGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12895..13122
                     /gene="yodD"
                     /locus_tag="OO332_002804"
     CDS             12895..13122
                     /gene="yodD"
                     /locus_tag="OO332_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5943224.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13420..14235
                     /locus_tag="OO332_002805"
                     /pseudo
     CDS             13420..14235
                     /locus_tag="OO332_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14232..15926)
                     /gene="yedQ"
                     /locus_tag="OO332_002806"
     CDS             complement(14232..15926)
                     /gene="yedQ"
                     /locus_tag="OO332_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR5943225.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         3967 a         4123 c         4211 g         3679 t
ORIGIN      
        1 tcaaggagaa ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca
       61 ccggcacaaa tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat
      121 ccccaggaaa gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc
      181 cctgtaggta tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata
      241 attgtgccaa cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta
      301 ccaatgacct ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct
      361 ttcctgttca tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc
      421 tagtgtatgt gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg
      481 atcagaagga accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca
      541 aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca
      601 ttttaaacca gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata
      661 taattaaaca agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct
      721 gatacgccgc caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt
      781 tttgcatatc ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt
      841 ctgcctgcgt gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct
      901 gcccggcaaa actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg
      961 tagcctgtaa ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc
     1021 ggataatttc tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca
     1081 acaaaaaaac gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca
     1141 atttttcgag tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc
     1201 ataatcagcc acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg
     1261 gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt
     1321 ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag
     1381 caatctttcc gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc
     1441 ttaccgcttc agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact
     1501 gcgcctgcgc ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct
     1561 cgatcaggaa aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct
     1621 ggaaggcgag ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca
     1681 tctggcaatg ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt
     1741 gacggtaaat ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca
     1801 tctggtttcc agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg
     1861 cgggcatctg ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa
     1921 atatgccagc gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat
     1981 tgttggtaac ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga
     2041 acaaacggaa gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg
     2101 ccagcttaat gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt
     2161 gtcgaatcaa ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa
     2221 taaccgccag cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa
     2281 tgaaaccagt aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga
     2341 tgtgcaacgt ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc
     2401 attgcctctc agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg
     2461 cttttctgaa aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga
     2521 cgaaagcggc ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc
     2581 tgccggtcgc tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg
     2641 cccgcagcta acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg
     2701 cgaggaagtg gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg
     2761 gcgcgctaac caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga
     2821 taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta
     2881 acctgacagg caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg
     2941 cagaggtgtt caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga
     3001 acgtcacgca gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag
     3061 ctgaacagtt tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca
     3121 aagctctggg tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata
     3181 ccgccagcgg tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc
     3241 gcgatgagca tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg
     3301 ccgatattct ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca
     3361 cctttggcgg cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc
     3421 tcgacggtca gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta
     3481 tcaacctgat gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg
     3541 gcgagctggc gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg
     3601 atgatcgcag cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc
     3661 tgaaaggggc cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg
     3721 ccgatattct gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa
     3781 acgaacagaa agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga
     3841 ttggcagcgg cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac
     3901 gatctcgcgc caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt
     3961 gaagaggccg aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag
     4021 cagggttatc aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag
     4081 gaaggactgg cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca
     4141 attcatgccc ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat
     4201 agtgtgatag cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt
     4261 cagacaccaa cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa
     4321 gaaccgttat tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg
     4381 gatgatatgc tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc
     4441 ctccaccctg gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc
     4501 actcgctggc aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc
     4561 tgactcgatg gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg
     4621 tacgtcgcta cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac
     4681 aattgccgct cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag
     4741 tagaaagcga agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag
     4801 tggaaggtgt cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc
     4861 aaagcggcaa gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg
     4921 gtaaaccgct cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc
     4981 cgccatttaa cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc
     5041 caatcaatgc cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg
     5101 gcgttggtaa aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg
     5161 tcgtgggttt gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg
     5221 gtgccgaagg acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc
     5281 tgcgaatgca aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc
     5341 agcatgtgtt gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg
     5401 cgctggcgat tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat
     5461 taccggcact ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt
     5521 tttataccgt gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg
     5581 caatcctcga cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg
     5641 ctatcgatat tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt
     5701 acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg
     5761 ttagcgtcgg cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt
     5821 ggccgcagct ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt
     5881 ctctccaggg gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga
     5941 tggcggaaca tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg
     6001 ccgctcgcct gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa
     6061 tgctgatcga ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga
     6121 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
     6181 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
     6241 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
     6301 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
     6361 ccgccatgag gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac
     6421 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag
     6481 cgaagcatta gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac
     6541 agataagccc acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca
     6601 agcggattta ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac
     6661 atcaacaccg ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac
     6721 gacaaaagac gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct
     6781 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt
     6841 gttaccggca gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc
     6901 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
     6961 ccagagtaaa gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct
     7021 aatcactcca caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc
     7081 gctgggttct cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca
     7141 ggggcaacaa agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc
     7201 cctcaaagtg gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg
     7261 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
     7321 gttagggcaa agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
     7381 gcaacagcaa agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac
     7441 gcttccggtt cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt
     7501 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
     7561 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
     7621 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
     7681 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
     7741 gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg
     7801 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat
     7861 gaagcaaccc gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta
     7921 ctgttttcac gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
     7981 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
     8041 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
     8101 tcaagctgaa attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc
     8161 cagtgttagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
     8221 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
     8281 ggggctgttc aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca
     8341 gccataccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct
     8401 gaaaccgcta cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt
     8461 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
     8521 ccatactgaa cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga
     8581 cgcctggaaa gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga
     8641 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
     8701 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
     8761 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
     8821 cgataacctg gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga
     8881 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga
     8941 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg
     9001 cactctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
     9061 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
     9121 gcaatggacg atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc
     9181 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
     9241 atcgacctga ttatggatat tccagtcaag ctgaccgtcg agctgggccg tacgcggatg
     9301 accatcaaag agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg
     9361 ggcgaaccgc tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt
     9421 gttgccgata aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc
     9481 cgcctgagcc gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg
     9541 ctgagccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
     9601 ctgcctggct ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga
     9661 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag
     9721 atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc
     9781 cttcagcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc
     9841 ttaagcgtgg cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg
     9901 gctgattatg cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg
     9961 cggtggacaa agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt
    10021 cattccagcg attttactga tgatgaccag tttcacccgc atcattattg tttttggttt
    10081 attgcgtaac gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc
    10141 actgtttttg acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta
    10201 ccagccattc agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc
    10261 gctgcgtgag tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact
    10321 ggcgaatacc ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc
    10381 ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt
    10441 tttgattatc gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc
    10501 ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca
    10561 attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga
    10621 atcggtcatg atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct
    10681 gttgctggta gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat
    10741 taacgaaatg acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat
    10801 tgccggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct
    10861 gccgtatatc atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc
    10921 tggttaagcc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca
    10981 attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg
    11041 ttcgccattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg
    11101 tggctggccg tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc
    11161 tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg
    11221 acatttaacg gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg
    11281 attggtagcg aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg
    11341 attttcctta acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat
    11401 ctggcattag gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt
    11461 ccattaactc tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct
    11521 ttttgcgaac atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta
    11581 ccattaatat aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata
    11641 tctgcgaaca tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat
    11701 atcgcaacat ttactttaag acaattccag gcaaattata caacacttta cgggatagta
    11761 agtccgcctg aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc
    11821 atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt
    11881 caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga
    11941 gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag
    12001 cttgtgattc acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg
    12061 cttctaacag tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg
    12121 tttttatggc aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat
    12181 tgatcagttc taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga
    12241 aaaaagagac aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg
    12301 aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa
    12361 tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa
    12421 cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta
    12481 tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata
    12541 agattactct gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca
    12601 gatgccgagc gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc
    12661 aactgccagt accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc
    12721 attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc
    12781 tggcagcgcc aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc
    12841 aggcggactt aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa
    12901 accgcaaaag tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg
    12961 ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa
    13021 cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa
    13081 ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc
    13141 ccgaccctga gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt
    13201 acaccaggaa atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa
    13261 tattcgcgct atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct
    13321 tggtggctct ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg
    13381 ttttgagaat catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca
    13441 ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa
    13501 ccggcagccc cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc
    13561 agtaaaactt cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg
    13621 ctgattgcag agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt
    13681 tttccacgca tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta
    13741 cgcgagaaag aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc
    13801 gaatggacgg gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta
    13861 acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa
    13921 ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga
    13981 aaagatcagg ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca
    14041 accacacttg gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac
    14101 gcggtgattg tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc
    14161 cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt
    14221 ttctccgccc gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg
    14281 aggtaaagcc gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc
    14341 tcgctgctac ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc
    14401 atctcttttt catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga
    14461 ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc
    14521 gcacgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga
    14581 tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa
    14641 ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca
    14701 cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga
    14761 acatacatgt tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta
    14821 aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct
    14881 tcgcttagcg tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta
    14941 acatagcgac tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc
    15001 aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc
    15061 aaaaatatca gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg
    15121 tttcttaaaa attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag
    15181 tagttattac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt
    15241 tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg
    15301 ataaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag
    15361 gtcggctgcg tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc
    15421 gacgaggagt tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca
    15481 ttgtcgaggc tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat
    15541 acgccattga ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg
    15601 cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg
    15661 agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc
    15721 ttatcgagcg cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct
    15781 tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag
    15841 cagagattaa cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg
    15901 ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct
    15961 ggcataaccc ggcaatagcg
//
LOCUS       DAJTRQ010000087        15835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000087 DAJTRQ010000000
VERSION     DAJTRQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..587
                     /locus_tag="OO332_002807"
     CDS             <1..587
                     /locus_tag="OO332_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5943226.1"
                     /translation="SSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWK
                     ALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWL
                     QSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFE
                     ELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            584..2035
                     /gene="djlC"
                     /locus_tag="OO332_002808"
     CDS             584..2035
                     /gene="djlC"
                     /locus_tag="OO332_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5943227.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2095..3765)
                     /gene="hscC"
                     /locus_tag="OO332_002809"
     CDS             complement(2095..3765)
                     /gene="hscC"
                     /locus_tag="OO332_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5943228.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="OO332_002810"
     CDS             complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="OO332_002810"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5943229.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="OO332_002811"
     CDS             complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="OO332_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5943230.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="OO332_002812"
     CDS             complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="OO332_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5943231.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="OO332_002813"
                     /pseudo
     CDS             complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="OO332_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="OO332_002814"
                     /pseudo
     CDS             complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="OO332_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="OO332_002815"
     CDS             complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="OO332_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5943232.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10078..10956)
                     /gene="corC"
                     /locus_tag="OO332_002816"
     CDS             complement(10078..10956)
                     /gene="corC"
                     /locus_tag="OO332_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5943233.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="OO332_002817"
     CDS             complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="OO332_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5943234.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11510..12550)
                     /locus_tag="OO332_002818"
     CDS             complement(11510..12550)
                     /locus_tag="OO332_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5943235.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="OO332_002819"
     CDS             complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="OO332_002819"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5943236.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14273..15448
                     /gene="ubiF"
                     /locus_tag="OO332_002820"
     CDS             14273..15448
                     /gene="ubiF"
                     /locus_tag="OO332_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5943237.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15602..15676)
                     /locus_tag="OO332_002821"
     tRNA            complement(15602..15676)
                     /locus_tag="OO332_002821"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15642..15644),aa:Gln,seq:ctg)
     gene            complement(15714..15788)
                     /locus_tag="OO332_002822"
     tRNA            complement(15714..15788)
                     /locus_tag="OO332_002822"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15754..15756),aa:Gln,seq:ctg)
BASE COUNT         3835 a         4048 c         4123 g         3829 t
ORIGIN      
        1 tctcatcatc ctggggaata aataatcgtg atgatttaat tcagactatt tatcagatga
       61 ctgatgatgg tcatgccaat gatttagctg gcttatatct gacatggcaa cgctcttccc
      121 ctgaagagtg gaaagcatta attgctggtg gctcggagag agggttgatt tatacccaat
      181 tcgttgccca aaccgctatg tgctgtggag aagggggaat caaagcctgg gattatgttc
      241 gaatggggtt tctgtcacgg gccggtgtgc tcaacaactg gctgacggaa gaagagagtt
      301 tatggctgca atcgcgcgtc tatgcacgag ctcaccatta ctatcacagc tggatgcact
      361 atttttctgc ctattcacta gggcgtctct actggcaatc ttctcagtgc gaagacaacg
      421 cttcgctacg cgaggcgcta accctgtaca aatacgatag cgccgggagt cggatgttcg
      481 aagagctggc agcgggaagc gaccgtttct atgccacgct tccctggcag ccattaactg
      541 tccagcctga atgcccggta acgcttaagg atgtgagcga cctatgaaaa catgttggca
      601 aatcctcgaa attgaaagca cgacgcaaat agacattatc cgccaggctt atcttgctcg
      661 cttaccgttg tgtcatcccg aaaccgatcc gcaggggttt aaagcattac gccaggccta
      721 tgaagaggcg ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga
      781 tgccaccgct gaacatgaaa tactacgtgc attcaggaca ttactggatt cagaaagtga
      841 tcgttttcag ccctccgcct ggcagaaatt tattcagcaa ttaaatacct ggaacatgga
      901 ggatgtcgat caattacgct ggccgctgtg tgcaatcgct atagaagcgc gatatctgtc
      961 attaaattgt gcttctttgt tggcagagcg tttgaactgg cattcgttta atgacagcga
     1021 aggaatggat gaggaagaaa gggaggcttt tcttgaggcc attcaggctg gtgattgttt
     1081 cgatttcctt agccttctgg aatatcccgt cgcgttacag aaccagactg ttgaatatta
     1141 cttcgcgctg gaacgttgct gccgttacca tcctgactat gtcactgcgt ttttggcgat
     1201 ggaaggaccg tggttcattc ctgatgatgc aaagttacat cgcaaactgt tgcgctggta
     1261 cagctcggtg caaacaggta tggtggaact cattcctgta gccaaacagt ggcaagcgga
     1321 agaaccagaa agcgaagatg cccggtatta ccagtgtgca caacgtttgt actgcggcga
     1381 gggggaaagc ctgcttgctg atctcggcgc gtactgggaa agttacccat ctacacaagc
     1441 tgataatttg ttgttgcagt ggagcaagcg tcattgcccg gattatttcg cgttattagt
     1501 gatggttatc gaagcgcgga gcatggtaga tgcgcaaggt caaccgctga aatatgttcc
     1561 tggtgagagc gcccggacgc ggctgttatg ggcggaaatt ttacatagcg gaaaattatc
     1621 gccgttaggt caatcgttta ttgagtcgtt attcttcaaa cgcaaagcat gggcgtggtg
     1681 gaaatcgaga gtcggtagcg agacagaaga agattcaccg ttactggatt tgtatcgggt
     1741 agcggaacag gtagtacttg aagcgtttcc gaaacaagag atgctggccc gtcttaatac
     1801 aaggctggaa ggcggagatg ctcatccatt agaggccatt atcacccgga tgctgttgac
     1861 gaaagtaaaa ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg aaaaccatga
     1921 agaaaaaaat gatgagggtg aaaaaccaca gagcattacc agcattatca aaatcagttt
     1981 aacggtgctg gttataggtt atgctctcgg caaaatcgcg atgttgttta gttgacgatg
     2041 tgttttttgc gccgatgccg gatgcagcat ttacgccgca tccggcagcc gtgattatga
     2101 ggtctcaatg gctaaataaa cgctcgcccg ccgcctgacc tcatcgacgc gggccatatc
     2161 gtttgactgc agcgccgcat caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc
     2221 acccagcgcc cgcgcccaca actcttccag tttggcttta aaggtgcgat tgatgagcat
     2281 atcgcgcgga taaattttca atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc
     2341 gctcaatgtt accgggctgt ggttaatcac tctggactta acgctgccgt cttccagaag
     2401 cacgtcaact tcaagcaacc cgttgatatc ataactaaag cgaatatcaa tcgactgata
     2461 agccccggtt ttcttcaacg gcacatcgaa ggattccacc agaatgttgt ttttaacttt
     2521 gtggttttct ccctgataga cgttaaccgt aattgaatcc tgttccgggt gcatggttga
     2581 ataagtttct acacgcgaca cgggcacagt ggtgtttcgt tcaataatcg gcgagaaaat
     2641 gccggaaacg ccctggcggt taacctcaac gcccaacgag taagggcaaa tatcagtgag
     2701 gatcacttct tcaatatctt cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag
     2761 cgcgacaatg gtgctcggat cgtaactttg atacggtaat ttgccaaaca gacgcacggc
     2821 gattcgctgc accagcggca tttgtgacgc gccgccaacc agcaccagac tgtcgatttg
     2881 actcggcttc agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag
     2941 cagcggcaac cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcatc
     3001 ctggtactgc cagcgaatgt gcattggcga ttgattgcta cattttgccg cttccacaca
     3061 ggcgtacaag gctgccagtt cactctcgtt aagcgtggtc cttgtgacat ccgcgcgctt
     3121 taaaacgtca tcgaccaaca tatgggtaaa atcttcgcca ccgaggaagt tgtcgccagc
     3181 ggaggcgtga acttcaatca ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc
     3241 gccaccgaga tcaaacacca gcgaacgggt attttgttgg gtatgcaggc catacgccat
     3301 cgcagctgct gtgggttcat taattaagcg taccgcattt aacccggcta agtccgctgc
     3361 taaacgggta tgcttgcgtt gtttatcgct gaaataagcc ggaacggaga tcaccacatc
     3421 tttaatcgga cgttgcagaa attcttcggc atcttctttt aatgagcgta ataccaaaga
     3481 ggacagttct ggcgcgttaa atgtgtcgct gcctaaccgc cagttggtat tactgcccat
     3541 tgcacgttta aataacgctg ccgttttatc cggatgcgaa gtacgccgtg atacagccgg
     3601 ttttccgact aaaatatgat tattttcatc catgctaatt atggatggtg ttaaatattc
     3661 accgaactta tttggaatta attgcgcggc accgtctttc cagacggcaa ttaaactatt
     3721 ggtagtgccg agatcgatac ctatggcgag ttctgcatta tccattgcat ttatatcttg
     3781 aaagaaaaag agataaatag attaagacca cccaggtaac gcgtggtgat cttaatcaat
     3841 gacgagtgtt aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga
     3901 tcaacatcga caattacagt ggcattcggt ttattgccag tcagataata ataatcaaca
     3961 accgtcatac ccagggtata tttcccctgt gtttccacgc caacccagcg ctcaacagtg
     4021 gtaaacaact cgggtttcaa cagccaggcg atggtgcatg ggtcatgcag cggtgcgccg
     4081 acaaagcccc atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca
     4141 atggttgaaa cagggttgcc aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgc
     4201 gctttatgag taacatccag accggccatc accaccggga tccctgactg gaagacaatt
     4261 tctgccgctt ccgggtcaac gtaaatatta aattcagccg caggcgtcca gttaccaagc
     4321 cccattgcgc cgcccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta
     4381 ttgagcagca aggcaacgtt agtttgcggt ccggtagaaa caatcgtgac aggttccgca
     4441 ctttcgcgca gcgttttcgc catcagctcc accgccgtac agttttgcgg tgcgaacgtc
     4501 ggttccggta atgccgggcc gtcgagaccg ctttcgccgt gcacattgtc cgcgataatc
     4561 aactcacgca ttaacggttt taccgcgccg cctgccaccg gaatatcggt gcgattaagc
     4621 aaggtcagca tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa
     4681 gacgtaattg ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg
     4741 tcatcatgac ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt
     4801 gtgcttcttt gcgacaaggg taacgccagg atgtaacaga tacgaggggc gaaacgataa
     4861 agcgtgagat ggcgcgcaat tgggtatgcg cgccagagtg attaatgcag gattttcgcg
     4921 aggaagtctt ttgcgcggtc cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct
     4981 tcgacaattt taccctcgtc cataaagatc acccgattcg ccactttgcg ggcaaagccc
     5041 atttcgtggg tcaccaccat catggtcatt ccttcgttcg ccagttccac catcacgtcc
     5101 agcacttcgt tgatcatctc cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca
     5161 ataggagcca tacacaatgc gcgagcgatt gccacacgct gctgctgacc gccggaaagc
     5221 tgcgccggaa atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct
     5281 ttttcacgag cgggcgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt
     5341 tcgataatcg acagatgagg gaacagctcg aaatgctgga ataccatccc gacgcgggag
     5401 cgcagctttg ccagatcggt tttcttgtcg ttaaccacga taccatcgac ggtgatttca
     5461 ccttgctgca ctggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca
     5521 gacgggccgc aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc
     5581 acctgaaagt gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc
     5641 ttttcaagta gctgaccaac aacgacgcgc taaggctaat aacgaaataa acaaatccgg
     5701 caaacaggat catctcgacc tgcgtaccat cacgctcacc aatggttgag gcggtacgga
     5761 agaaatcggc cagagataac acatacacca gcgaggtatc ctggaacagt acgatgccct
     5821 gagtgagcag cagcggcacc atcgcgcgga aagcctgcgg cagaataatc agtttcatcg
     5881 actgccagtg agtcatcccc aacgccagcg cggcgctcga ctgaccacgg gaaatacttt
     5941 gaataccggc acggataatc tctgaatagt aggccgcttc aaacatcgaa aacgccacca
     6001 tcgccgaaat taaacgaata tcattttttg gcgataatcc cagcacattt tgcagaaatc
     6061 ccggcacaat caagtaaaac cacagcaaaa ccatgactaa aggaatcgag cggaatacgt
     6121 taacgtaggc tttggcaaac cacgaaacag gcgcgaagct ggacaagcgc atcaccgcca
     6181 gcatggtacc ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agggtgatca
     6241 ccagcccgtc gagcagatat ggcagggaag gaacaatgga actccagtca aactcgtaca
     6301 ttatttgccc cccatgttgc cgggcaggcg aactttacgt tcaaccagcg tcatcaccag
     6361 catgataaaa gcgttaatca acacatacgt aagcgtgatg gcggtaaacg actcccaggc
     6421 gtgagccgag tagtcgagca atttacccgc ctgcgccgcc atatccacca gaccgatggt
     6481 cgaggcgatg gcggagtttt tcaccaggtt catcatctcg gaagtcatcg gcgggacgat
     6541 aacgcgataa gcattaggca gcagtacgta tcgataagtc tgcggtagcg tcaggcccat
     6601 cgccagcgcg gcattcttct gcccacgcgg tagcgactga atagcggcgc gtacctgttc
     6661 gcaaacacgg gaggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat
     6721 attgggatcc agctcggcct taaaccacat gccgattttc tccggcagca gctccgggat
     6781 caccagatac caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata
     6841 caacgtaccc agaccggaga gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga
     6901 accgacgagg aaagcgataa ttcaggcgca gatcgacaaa gcgatcgtca cctgaaaacc
     6961 actccagacc caaccaagat aggtggtatt gccgaacggg gcttgttgta aaaaaatacc
     7021 ccagttccag tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg
     7081 ctaccctcga agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca
     7141 tcaggcgtat agtctgtccg tgctacgtaa caatcgagag ggctggaatt tccgcctctg
     7201 gttcttgtaa ttagttcagt gccttgtcat tcggttcttt gaacagtgct ttcatttcgt
     7261 ctgacagttc gaaattcatg ttcaggtttt tcggcggaat tggatttttg aaccatttat
     7321 caaaccattt ttccgcttca ccggaggtct gcacctgagc gatagtgtca tccatcagct
     7381 ttttgaactg cggatcgtct ttacgcaaca tgcaaccgta ggcttcctga gactgcggct
     7441 tgccgacgat atcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcagag
     7501 cgtcatccat cataaaggca acggcacgac cgctttccag ggtgcggaaa gagtcaccgt
     7561 gatctttggc gctgatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca
     7621 aaacttcaga ggtagtgccg gaagtgacga ctacggcttt gcctttcagg tcggcaaaat
     7681 ctttgatatc gccgcccttt ttggtcaaca ggcgcgtacc gaccacgaaa atagtgtcag
     7741 agaaagccgc ctatttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcga
     7801 aagtgccgtt ttgcagcagt ggaatacggt ttgtgaggta atcggaatca gttttacctg
     7861 caagtccggt ttgttgagtt tctttttcac tgcttcaaca atggcgttgg agtaatcctg
     7921 cgagtaaccc accacttttt gctgattgtc gtaataagag aaaggtactg aagattcacg
     7981 gtgaccgacg acaatcacac cgtttttggc gattttgtcc agcgtactgc ctgctgccgg
     8041 ggcggcgtca tctgcctgtg ccagtcctgc ggaaagcgcc agggcgagga ttgctgtggc
     8101 aggtttacgt aattgcatat ccaactcctt tatcttctgc gttaagaacg catggatacc
     8161 cgttgtgagt gtttgtgttg ttatcgtctg caactttatt gtgcagtgtt gtgcctgtta
     8221 ggtaacattt agtttggcta aatgtaaaga tattgatgtt ttattgtttg tttttgcgag
     8281 gtgcaccgca ccattctgaa gcaaaattct taaaatgcac tcttttggtg ctaccggtgg
     8341 attgctgtgg tgcaaccggg ttgtactgat gctggttcaa gctttgcctt gtataacaaa
     8401 gcaatagatg tgccaaagtt ggataggaga atattgttat ccggataatg cactgatgcc
     8461 gcatccggtg agcgtgccga aatatgggat gtattccggc acgataagaa gggattattt
     8521 acgtcgctga cgcagactca tcaacacagc agcaaaacca aacagtgccg tcagcaccca
     8581 cagcggccag ttgccggtac gtgcgtatgg tgtgagtccg gtggtcggcg tcacgttagt
     8641 ggttaacacc tcgcgggtga actgcgggat catcgcctga atctcaccct gcgggccaat
     8701 caccgccgta atgccgttgt tggtgctgcg caacagtggg cgcgccagct ccagcgcacg
     8761 cattcgcgcc atctggaagt gttgccatgg accaatagat ttaccaaacc acgcatcgtt
     8821 ggagatagtc agcaggtagt cggtatccgg gcggaagtta tcgcgcactt gctcgccgag
     8881 aatgatctcg tagcaaatag ccgcagtaag ctcaatacca tttgccgaca gcggcggctg
     8941 gatatatggc ccacggctga acgacgacat cggcagatca aagaacggtg ctaacggacg
     9001 cagaatcgac tccagcggga caaactcgcc aaacggcacc agatggtttt tgttatagcg
     9061 atcggctgat tcgtagctgt acggtgcacc tttacccagc gtgatgatgg tgttgtaggt
     9121 atcgtagcgg ttctgcttat tgagacgcgc gtcgacaatc ccggttacca gcgagctacc
     9181 tttatcacgc aactcaccgt ccagtgcttt gaggaacggt tgctgattaa tttccagatc
     9241 ggttatcgcc gactccggcc agataatcaa cgatgatttg cccatcagcg gtgccgttgc
     9301 gttgtagtaa atctttagcg tattaagaag ctggccttcg tcccatttca gcgattgcgg
     9361 aatatcgccc tgaaccatcg aaacctgaat ggttttctcc ggttgcgggg taaaccattg
     9421 gatgtaacgc agcgggaagg gtagggcaaa cagcacgacg gccaccacca gcggacgcca
     9481 gttgcgtttg accaacgcca gtgccagcag gccactaacc atcatcagca ggaagttaat
     9541 ggcttccacg cccattagcg gtgccagccc ttttaacggg ccatcaatct ggctatagcc
     9601 gaactgtaac cacgggaagc cagtcagcac ccaaccgcgc agaaactcgg tcacttgcca
     9661 gagagcaggg gcggcaatcg ccacgcgcag ccaggtggtt ttcggccaca gacgcgacag
     9721 cacgccagca aacagtccgg tatacagcga caaatacgcc gccagcagca ccactaggaa
     9781 gatgttaacc gggccaggca tcccgccaaa ggtcgcgatg ctgacataga cccagttaat
     9841 accgctgcca aagaggccaa atccccagca aaagccaata gcggcagact ggagtgggcg
     9901 acggttaaag gtcaacgcct gaagccccat cagcgaaata atcgccgcag gccagacgtc
     9961 gtaaggagag aaggccagcg ttccgcaggc accgaataat aacgccagca gcaggcgaat
    10021 gcgctggcgt tcaattaatg aggcaaaagc catgtagtta tctatccagt ttcggtttta
    10081 ttcatccagc tttggctggg gtgagtcatc cgggattttg acatgaacct gaataatacg
    10141 ccgactatcg gccatcgcca ctttgaactg gtaaccgtcg atgtcgatag tttcgccacg
    10201 cgccggaaga tgcccaaacg cctgcatcac cagaccaccg atagtgtcga cttcttcatc
    10261 gctaaagtgg gtgccgaacg cttcgttgaa gtcttcaatg gaagccagtg cgcgcacggt
    10321 ccaggtatga cgactcagct gacggaagtc gatatcatct tcttcgtcat actcgtcttc
    10381 aatctcacca acaatcagtt ccaggatgtc ttcaatggtc accagaccgg aaaccccacc
    10441 gaattcgtca ataacgatcg ccatgtggta acgctgagag cgaaactctt tcagcatccg
    10501 gtctacgcgc ttactttcag gaacgacaac cgcctgacgt aacactttgt ccatgccgaa
    10561 ggcttcagca tcgctgcgca taaacggcag caagtctttc gccatcagaa tcccttcaat
    10621 gtgatctttg tcttcgctaa tcaccgggaa acgtgagtgg gcggactcga tgatgacatc
    10681 aaggcattcg tccagcgtct ggttgcgttt cagggtaatc atctgggagc gggggatcat
    10741 gatgtcgcga acgcgttggt ctgcgatgtc catcacccct tcgagcatat cgcgcgtatc
    10801 ttcgtcgata aggtcgttct gcccggaatc acggatcagc gccagcagtt cgtcacggtt
    10861 tttcggttca ccgtggaaaa gttggctgag taacagggag aaaaatccct tcttgttgct
    10921 tatcgtgtca ctactgtgtg aattgtcgtc gctcatggcg tcgtatgggt tctcatgtta
    10981 gttaatcaaa acgccgtcgt taatcaccaa cggcggggac gtctgccagt caaatgcctg
    11041 gcaaattatt ctttctcggc aatgtacgga tcctcatagc ccagagcaag cataatctct
    11101 gtttcgaggg cttccatttc ttctgcttcg tcatcttcaa tgtgatcgta acctaacaaa
    11161 tgcagactgc cgtgcaccac catatgcgcc cagtgcgcct ccagtggttt gccttgctcc
    11221 tgagcttcct tctcaaccac ctgacggcag ataaccagat cgcccagtag cgacatctcc
    11281 atgccaggcg gcacttcaaa cgggaaggag agcacgttgg tcggcttatc cttaccgcgg
    11341 taggtcagat tcagactgtg gctttcggcg gtatcgacca cgcgaatagt cacttccgac
    11401 tcttcctgaa actgcgggat caccgcattc agccatgttt gaaactggct ctcttccggt
    11461 aacccggaat tatcttcaca tgccagttgt aaatcgagga tcacctgact catttttgtt
    11521 cctgttcttc gcgcttgcgt tctgctgcca gcgccgcttt acgtttttgt tctgcttctt
    11581 ccctggcttc ataagcgtta acgatacgcg ccaccaccgg gtgacgaacc acgtcttcgc
    11641 tgtggaagaa gttaaagctg atctcttcga catcggccag cacttcaatg gcatgacgta
    11701 agcctgattt agtattacgc ggcaggtcga tctgtgtgac gtcgccggtg ataaccgctt
    11761 ttgagttaaa accgatacgg gtcaggaaca tcttcatctg ttcgatggta gtgttctggc
    11821 tctcatcgag aatgataaac gcgtcgttca gcgtacgacc acgcatatag gccagcggcg
    11881 cgacttcaat aacgttgcgc tcaatcagtt tctcgacttt ctcaaagccc agcatttcaa
    11941 acagcgcgtc gtacagtggg cgcagatacg ggtctacttt ctggcttaaa tcgccaggca
    12001 ggaagcccag tttctcaccg gcttcgaccg ccggacgagt cagcagaata cggcgaattt
    12061 cctgacgctc cagggcatca actgccgcag ccactgccag gtaggtttta cccgtacccg
    12121 ccgggccaac gccgaaggta atgtcatggt cgagaatatt ggcgatgtac tgcgcctggt
    12181 ttggcgtgcg cggcttaatt acgccgcgtt tagttttgat attgaccgct ttgccgtact
    12241 ccggcacgct ctccgcgctt tgctccagta cccgcgcttc tttaatcgca aggtggatct
    12301 gttccggttc gatatcctga atctgaccgc gcatcggggc agtatcgaca tacaggctac
    12361 gcagaatgtc tgccgcagcg gtgacgcaaa tcggacggcc ggtcagttta aagtggttat
    12421 cgcggcgatt gatctcgatg ccgagacggc gttcgagctg cttgatgttg tcatcaaacg
    12481 ggccgcacag gctcaacaga cgcgcattgt ctgctggctc cagggtgatt tcgcgagtgt
    12541 ctatgttcaa accgttcctc ttatctgtat gccgccggaa gctgaacatt caccggccta
    12601 taaggaaatt attcacgcca caggaaaaag gcgcaagcat tgcgataaag atggggataa
    12661 agagagaaaa aacaaggccc accggaacgg caggcctgag aattacggct gataataacc
    12721 cacgccaagg tcgttttctt tgcgggtgcg ggcaatcacc gactccggtg tttctgccat
    12781 gcgcagaccc atttcatctt cagttcgcac cactttaccg cgcagagagt tcgggtagac
    12841 gtcggtaatt tctacatcga cgaatttacc gatcatatcc ggtgtgccct cgaagttgac
    12901 cacgcggtta ttttcggtac gcccggaaag ctccatgatg ctcttacgcg aagtcccttc
    12961 taccagaata cgctgggtgg tgccgagcat ccgacggctc catgccatcg cttgctgatt
    13021 gatacgttct tgcagaatat acagacgctg cttcttctct tcttccggaa catcatcaac
    13081 catatcggcg gcaggcgttc ctggacgtgc agagaagata aagctgtagc tcatgtcgaa
    13141 attgacgtcg gcaatcagct tcatcgtttt ctcgaagtct tcggtggttt cgccagggaa
    13201 gccaacaatg aagtcagaac tgatctgaat atccggacgc gccgcacgca gtttacggat
    13261 gatcgctttg tactccagcg ccgtatgggt acgtcccatc aggttcagaa tgcgatcgga
    13321 accgctctgt accggcagat gcaggaagct caccagctca ggcgtgtcgc ggtacacttc
    13381 gatgatatcg tcggtgaatt cgatcggatg gctggtggta aagcgaatac gatcgatccc
    13441 gtcgatcgca gcaaccagac gcaacagatc ggcaaacgat ccggtggtgc cgtcgtagtt
    13501 ctcaccacgc caggcattca cattctgacc gagcaggttg acttcacgca cgccctgagc
    13561 cgcaagctgg gcaatctcaa acagaatatc gtcagacgga cggcttacct cttcaccacg
    13621 ggtgtaaggc accacgcagt aggtgcaata tttattgcag ccttccatga tggagacaaa
    13681 cgcggtcggc ccttcggcgc gcggttccgg cagacggtca aacttctcga tttccgggaa
    13741 gctgatatct acaaccgggc tgcggtcgcc gcgcacggag ttgatcatct ccggcagacg
    13801 gtgcagcgtt tgcggcccaa aaataatatc gacatagtgg gcgcgctggc gaatgtgctc
    13861 gccttcttgc gatgccacgc agccaccgac gccgataatc aggtctggat tcttctcttt
    13921 taacagtttc cagcgaccca actgatggaa gactttttcc tgagccttct cgcggattga
    13981 gcaggtgttc agcagtagca catccgcttc ttccgccacg tcggtcagtt gatagccgtg
    14041 ggtggcatcc agcagatcgg ccatcttcga tgaatcgtac tcgttcatct gacagcccca
    14101 ggttttaata tggagttttt tggtcatcga cttgctcttg cgaaatagta gccaggaatg
    14161 cagggcgcat agtgtaatgc tttgctgccg ttgtgaccag tatgagcgtt atcagccctt
    14221 gggggtaaaa atcctgtaaa cttaaagcat attgctaaca ggatgattga ccatgacaaa
    14281 tcaaccaacg gaaattgcca ttgtcggcgg aggaatggtc ggcggcgcac tggcgctggg
    14341 gctggcacag cacggatttt cggtaacggt gatcgagcac gcagaacctg caccgtttgt
    14401 cgctgacagc caaccggacg tgcggatctc ggcgatcagc gcggcttcgg tatcattgct
    14461 taaaggggta ggggtctggg atgcagtaca ggctatgcgt tgccatcctt accgcagact
    14521 ggaaacctgg gagtgggaaa cggcacatgt ggtatttgac gcagccgaat taaagttgcc
    14581 gttgcttggc tacatggtgg aaaacacagt cctgcaacag gcgttgtggc aggcgctgga
    14641 agcgcatccg aaagtaacgt tacgtgtgcc aacctcgctg attgcattac atcgagataa
    14701 tgatcttcag gagctggaac tgaagggcgg tgaaacgatc cgcgcgaaac tggtgattgg
    14761 tgccgacggc gcaaattctc aggtgcggca gatggcggga attggcgttc atgcctggca
    14821 gtatgcgcag tcgtgcatgt tgattagcgt acagtgtgag aacgatcccg gcgacagcac
    14881 ctggcagcaa tttaccccgg acggaccgcg tgcgtttctg ccgttgtttg ataactgggc
    14941 atcgctggtg tggtatgact ctccggcgcg tattcgccag ttgcagaata tgaatatggc
    15001 gcaactccag gcggaaatcg cgaagcattt cccgtcgcgt ctgggttacg ttacaccgct
    15061 tgccgctggt gcgtttccgc tgacgcgccg acatgcgttg cagtatgtgc agccggggct
    15121 tgcgctggtg ggcgatgccg cgcataccat ccatccgctg gcggggcagg gggtgaatct
    15181 tggttatcgt gatgtcgatg ccctgattga tgttctggtg aatgcccgca gctacggtga
    15241 agcgtgggcc agttatcctg tcctcaagcg ttaccagatg cggcgcatgg cggataactt
    15301 cattatgcaa agtggtatgg atctgtttta tgccggattc agtaataatc tgccgccgct
    15361 gcgttttgtg cgtaatctcg ggttaatggc ggcagagcgt gctggcgtgt tgaaacgtca
    15421 ggcgctgaaa tatgcgttag ggttgtagcc ttacaacatt gccggatgcg tgccaaccgt
    15481 aggtcggata agacgcggca gcgtcgcatc cgacattgaa ggataagacg tgtcaacatc
    15541 gcattcgaca ttgaatgaac gcagaaaagc aaaaagctcg ccgaagcgag cttttttaat
    15601 gtggctgggg tacgaggatt cgaacctcgg aatgccggaa tcagaatccg gtgccttacc
    15661 gcttggcgat accccaactg ggtgcactta cgatgtaagc gtcttgaata aattggctgg
    15721 ggtacgagga ttcgaacctc ggaatgccgg aatcagaatc cggtgcctta ccgcttggcg
    15781 ataccccaac aaattggttt ttgaatttgc cgaacgtatt cgacacattc agaat
//
LOCUS       DAJTRQ010000088        15817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000088 DAJTRQ010000000
VERSION     DAJTRQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..870)
                     /locus_tag="OO332_002823"
     CDS             complement(<1..870)
                     /locus_tag="OO332_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5943238.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNSFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIG"
     gene            977..1273
                     /locus_tag="OO332_002824"
     CDS             977..1273
                     /locus_tag="OO332_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5943239.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1407..1832)
                     /gene="arsC"
                     /locus_tag="OO332_002825"
     CDS             complement(1407..1832)
                     /gene="arsC"
                     /locus_tag="OO332_002825"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5943240.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1845..3134)
                     /gene="arsB"
                     /locus_tag="OO332_002826"
     CDS             complement(1845..3134)
                     /gene="arsB"
                     /locus_tag="OO332_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5943241.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3188..3541)
                     /gene="arsR"
                     /locus_tag="OO332_002827"
     CDS             complement(3188..3541)
                     /gene="arsR"
                     /locus_tag="OO332_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5943242.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4286..4369
                     /locus_tag="OO332_002828"
     CDS             4286..4369
                     /locus_tag="OO332_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5943243.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4423..5775)
                     /gene="gorA"
                     /locus_tag="OO332_002829"
     CDS             complement(4423..5775)
                     /gene="gorA"
                     /locus_tag="OO332_002829"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5943244.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5847..6689)
                     /gene="rlmJ"
                     /locus_tag="OO332_002830"
     CDS             complement(5847..6689)
                     /gene="rlmJ"
                     /locus_tag="OO332_002830"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5943245.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6892..8934
                     /gene="prlC"
                     /locus_tag="OO332_002831"
     CDS             6892..8934
                     /gene="prlC"
                     /locus_tag="OO332_002831"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5943246.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8942..9694
                     /gene="rsmJ"
                     /locus_tag="OO332_002832"
     CDS             8942..9694
                     /gene="rsmJ"
                     /locus_tag="OO332_002832"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5943247.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9743..11212)
                     /gene="dtpB"
                     /locus_tag="OO332_002833"
                     /pseudo
     CDS             complement(9743..11212)
                     /gene="dtpB"
                     /locus_tag="OO332_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11518..11952)
                     /gene="uspA"
                     /locus_tag="OO332_002834"
     CDS             complement(11518..11952)
                     /gene="uspA"
                     /locus_tag="OO332_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5943248.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12343..12678
                     /gene="uspB"
                     /locus_tag="OO332_002835"
     CDS             12343..12678
                     /gene="uspB"
                     /locus_tag="OO332_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5943249.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12749..14248)
                     /gene="pitA"
                     /locus_tag="OO332_002836"
     CDS             complement(12749..14248)
                     /gene="pitA"
                     /locus_tag="OO332_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5943250.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14480..15679
                     /locus_tag="OO332_002837"
     CDS             14480..15679
                     /locus_tag="OO332_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5943251.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3982 a         4043 c         4149 g         3643 t
ORIGIN      
        1 gccgatgatt aatgccatta atgctcccag acccattcct tttgtcatca gaacagatgc
       61 cagagggata accgcctcag cccggatata cagcggaata ccgacaacgg cgcttaatgg
      121 gatggcgaag gaattatctg ctcctgcatg agcggcaatc cacgctgagg gaataaagcc
      181 ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat ctttaaactg
      241 ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag ctttggtact
      301 gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac aactcacttt
      361 tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat ttgctgatga
      421 gcttttactg gcaatgatat gacgttcaaa ccccagagaa tccaggataa tactggcaag
      481 aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac caaaggtcat
      541 ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa aagtgagagt
      601 cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg tcgagcaact
      661 acagaacggg gtaacggctc ccaacagagc agccaggaga tagcctctcc cttttctggc
      721 ccccatcatc tgctggattt tatggtctgg caccttttgt cttatcaggc tgacaccggc
      781 actaatcact ataaacagca acgaaagctc gacggcgaga aacacaaaca tctctgcggc
      841 atcttgcagc atcgcaagcc aactactcat acgtaacctc attgggtaaa tctatatttc
      901 cagaataatc gaaatgaaat tcagatcaag atatttctgg tattatcgaa atatcaacca
      961 gacagagggg tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag aactaggtca
     1021 cccgacccgt ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag ggttgccggt
     1081 cggtgagttg caaaagcagc ttgggatacc tggctccaca ctcagccacc atatatcagc
     1141 actggtatcg gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga tgtgtgtttc
     1201 gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct gcgtaaatag
     1261 caaaattgcc tgatgcgcta cgctctacat atttcatgcc atatattgaa tttgcacggt
     1321 tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc gcactttacc
     1381 aactatcggc agtggatatc gccgctttat ttcaggcgct tacccgcttc atcaaccact
     1441 ttctcgccat cttccttgga gaatgcgcct ttttgcgcat ctggcagaat ttccagcacc
     1501 acttctgaag ggcggcacag gcgagttccc agcggcgtca ccacaatcgg gcgattaatc
     1561 agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt atcttccgca
     1621 aggcccagct cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac ggaaatcccc
     1681 atatcggcaa tgagtttgac cagttcatcg cgcgttggcg gagtttccag ataatggata
     1741 atggtcggtt ctgtgccgct gttgcggatc atctccagcg tattacgcga cgtgccgcag
     1801 gccgggttgt gataaatggt aatgttgctc atatcagtat ctcattacaa agtgaaagag
     1861 agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat gataatccct
     1921 gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac gtgcagccag
     1981 agcagcgttg ccaggctacc aatcggggta attttcggcc ccaaatcgca gccaatcacg
     2041 ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc aatggacaac
     2101 gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc ggtgaggaat
     2161 ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag tacgccagaa
     2221 agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat gccgagcgag
     2281 aagatgacaa tctgccaggg ggcaccgcgc aggactttac ccgtattaat cgcatgaccg
     2341 cgtttagcga cgacaaataa tatcagtgcg cccacagctg caatggcgct caccggaatg
     2401 ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac ccagccagtt
     2461 ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagcagcgc catatcgtag
     2521 ttctgcggaa tatctttgcg aaaatagaga tgtagcatca ccagcgtggc aacaatcgcg
     2581 gcgatatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc aaagaaatca
     2641 gcggaaacga tattcaccaa gttggagaca ataagcggca ggctggcggt atcggcaatg
     2701 aatccggccg ccatcacgaa cgccagcgtc gtgcctttac tgaatcctaa agccagcagc
     2761 atggcgatga caatcggcgt caaaataagc gccgcgccat cattggcaaa cagggccgca
     2821 acggcggcac cgagcaggac aatccaggta aacagcaagc gaccgcgtcc attgccccag
     2881 cgtgaaacgt gcagcgctgc ccattcaaaa aagccggact catccagcag caggctgatg
     2941 ataatgacgg caataaacgc cgccgtcgcg ttccagacga tattccacac caccggaata
     3001 tcacccggat gaaccacgcc cgtaaccaac gccagtaccg cgccgagcgt tgcactccag
     3061 ccgatgccta aacctttcgg ctgccagata accaatacga tggtcaggac aaagatagcg
     3121 cctgccagta acataatgcc tcccggataa aacacatgtg caaatccata tgtgtttagc
     3181 taaattttta actgcaaatg ttcttactgt ctccggaaca gttttgtctg gccaggttgc
     3241 ggacaattgc ctgaatcttt tcctgttcac atcgccaggc ttcatcaata attttcgccg
     3301 cccatgctgg aatatgcggc gataagcggt aatgaaccca cttaccttgc ttgcggtcca
     3361 gcaatagccc gctttcacgc agcaatgcca gatggcggga tatcttgggc tgcgactgat
     3421 cgagggcagt gcagagatcg cagacgcata actctcccag ttcgctaagc agtaaaacga
     3481 tgcccagacg ggtttcatca gcaagaattt tgaacaattg gatgggtaac agaaatgaca
     3541 tattgcgctc ctgattgttg caggtagtgt ctctcttcga agcggataag tcaaaaacat
     3601 atatgactta acgaatgtgt aagtgcagag gaaggtaata ggtgtgaatt ttgagttggc
     3661 tattcatttg aaaggcggtt tgaatcagga cgtaaaaaaa accgactttg cgtcggtttt
     3721 tttactcttc agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt
     3781 tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca gctggagaag
     3841 ggacagtttg ctatacatgg tggtgaaaga ggatttatat actttcatcg ggtcgctgcc
     3901 gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac ttcgcgtcgg tttttttact
     3961 cttcagccca gagttggtgg ctctggctgg agtgagaatg ccatcatcta acgcatcaat
     4021 gctaatacca tactgaaact attgcaaggt tatgctggtt atttagccta attacactgt
     4081 atgattatcc agttagctct gaggcatttt cactctggca atgcgcataa acgctttcaa
     4141 agtcctggtc agaagtacgg gtggtgccgt taactgatgc tctggccgga gtgagattgc
     4201 ccacatctaa caatgaggca tgcgccgtga caggcagtgg atgagtaagc ggatgcattc
     4261 tcactccatc gcatggagaa aacgggtgat tgataaagca atcatcgttc ttggggcgtt
     4321 aattgtgctg ctggaactga tccgctttct gcttcagctt ctgaactgat agcggaaacg
     4381 taattaaggg ctaagagcac actactctta gccctttaac atttaacgca tggtcacgaa
     4441 ctcttctgcc gccgttgggt gaatggcgac ggtattgtcg aagtcttttt tggttgcgcc
     4501 catcttcagc gccaccgcga agccctgcaa catttcgtcc ataccaaagc caatgccgtg
     4561 aataccgaca atcttctctt ctggcccaac gcacaccagc ttcatgcggc acggctggcg
     4621 gtgagtggtg acggcggtat acatcgcggt gaaagaggat ttatacactt tcacctgatc
     4681 gtcgccgtac tgctcgcgcg cctgcggttc cgttaaacca acagtaccaa tcggcggatg
     4741 gctgaagacc acggtcggaa tgttgctgta atccagatgc tcatccggct tgttattaaa
     4801 caagcgttca gagagacggc gacccgctgc aactgccacc ggtgtcagtt ccactgcacc
     4861 cgtgttatcg cccaccgcgt aaataccttc aatattggtg ttttgatatt tatcgacgac
     4921 gatatagcct ttttcgttgg ttttaacgcc agcggcttcg aggttgatgt tgtcattggc
     4981 aggctcgcga ccaatcgccc aaatcaggca atccaccgtt tcactgcgac catcttccag
     5041 ctccagcgtc aggctaccat cggcattttt cactaccgct ttcgggatgg cgttggtatg
     5101 caactgtggg ccttcggcgt tcatcacttc gaccagcgtt tcagagatca tcgggtcgaa
     5161 gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtt ttcgcgccga ggccgttaat
     5221 cacgccagcc agctcaacgg cgatgtaacc cgcgccaaca accgccacgc gctctggcaa
     5281 agcaggaagg gcgaagaagc catcggaatc aataccgtat tccacgcctg gaatatccgg
     5341 gtggctcgga cgaccgccgg tggcgatcag aatatgatcg gccgtgatgg tttcgccgtt
     5401 aacttccagc gttttggcat caacgaagcg ggcaaagcct ttgattacat caacgttatt
     5461 tttaccgagc acgttttcat aggaagtatg aatacggtcg atataggcgg tacggctggc
     5521 gatcaacgtt tcccagttga acttgttgat ggtggtatcg aaaccataat cagggccgta
     5581 catatggatc gcttcacgga tttgcgccgc gtgccacatc acttttttcg gcacgcagcc
     5641 gacgttcacg caggtgccgc ccagctcttt ggcttcaatc agtgcgcatt tctggccgta
     5701 catagccgcg cggttgatgg aggcgatacc gccgctgccg ccgccgatgg cgatgtaatc
     5761 atagtgttta gtcatgacat attgtcctta tcgttgatta ccgcgattgt agcgcgagac
     5821 gtaataggtg ccagcaatgg ctgcaattac tccggcacga tccagcttac ggtggcgtgc
     5881 ccggtacctg ccggaaccag tttgctgtgc agccacgaca gcacgttatt catctgctgt
     5941 tccagcttcc acggcgggtt aatcacaatc atgccggaag cggtcatgcc acggcgatcg
     6001 ctgtctggca gcaccgccag ttcaatttgc agaattttgc gaatgccggt cgcttccagg
     6061 tcgtggatca tgcgcttaat ttgctggcgc agcaccaccg gataccacaa tgcgtaagtg
     6121 ccagtggcga aacgtttgta accttctgct atcccgctga ccaccgcctg atagtcggtt
     6181 ttcatttcat acggcgggtc gataaggatt aaaccacggc gagaaaccgg cggcagtttg
     6241 gccttaagtt gctggaaacc gtcggctttt tcgacgcgtg cacggctgtc tttctgaaat
     6301 ccagaacgca gcaacgggta atcgctcggg tgcagttcgg tcagttgcag gctgtcttgt
     6361 tcgcgcagta gctggcgagc aatcaacggc gaaccggggt agtaacgcaa ctggccgcta
     6421 cggttgaagt gttttaccac attgatgtac gcctccagtt ctgcgggcaa atcgtcctgc
     6481 tgccagatac gggcgatacc ttcgagatat tcgccggtac gctcggcatg ttcgctacct
     6541 aactgataac gcccggcccc tgcgtgggtg tcgagataga gaaacggttt atctttctct
     6601 ttcagcgact cgatgatcag gctctgaacg gtatgtttaa ggacgtcggc gtggttgcca
     6661 gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg ttccggtaag taaaaattgg
     6721 cccgtattcg gggcgcacaa aagcctatca ggacagtata ccgaaaagag gccgccgccg
     6781 cgaaagcgta acgtttccca ttgaaattca ctacacttaa ccccatgcta cacacattat
     6841 gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca ggactgcgct aatgacgaat
     6901 ccgttactga ctccctttga attgcctccg ttttctaaaa ttctcccgga acatgtcgtt
     6961 ccagccgtga ctaaggcatt gaacgactgc cgcgaaaatg tggagcgcgt agtagcgcaa
     7021 ggggcaccgt acacctggga aaatctctgc cagccgttgg cggaagtgga cgatgttttg
     7081 gggcgtatct tctccccggt cagccacctg aactcggtga aaaatagccc ggaactgcgt
     7141 gaagcgtacg aacaaaccct gccgctgctg tcggaataca gcacctgggt agggcaacat
     7201 gaagggctgt ataaagcgta ccgcgacctg cgcgatggcg atcattacgc cacgctgaac
     7261 acggcgcaga agaaagcggt tgataacgcc ctgcgtgatt ttgaactctc tggcataggt
     7321 ctgccgaaag agaaacagca gcgttacggc gaaattgcta cccgtctttc tgaactgggc
     7381 aaccagtaca gcaacaacgt cctcgatgcg acgatgggct ggaccaaact cgttaccgac
     7441 gaagcggggc tggcggggat gccagaaagc gcgctggctg cggcaaaagc ccaggccgaa
     7501 gcgaaagagc tggaaggcta cctgctgacg ctggatatcc caagctacct gccggtaatg
     7561 acctactgcg acaaccaggc tctgcgcgaa gagatgtatc gcgcttacag cacccgcgcc
     7621 tccgatcaag gcccgaacgc cggtaagtgg gataacagca aggtgatgga agagatcctc
     7681 gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa actacgcctt taaatccctt
     7741 gccactaaaa tggcagaaaa cccgcagcag gtgctggatt tcttaaccga tctggcaaaa
     7801 cgcgcgcgtc cgcaaggcga aaaagagctg gagcaactgc gtgccttcgc taaagccgaa
     7861 tttggtgtcg atgagttgca gccgtgggat atcgcttact acagcgaaaa acagaaacag
     7921 cacctctaca gcatcagcga cgagcagctg cgtccgtact tcccggaaaa caaagcggtt
     7981 aacggcctgt ttgaagtggt gaaacgtatt tacggcatca ccgctaaaga gcgtaaagat
     8041 gttgatgtct ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa taacgaactg
     8101 cgcggcagct tctacctcga tctgtatgcc cgtgaaaaca agcgcggcgg ggcgtggatg
     8161 gatggctgcg taggccagat gcgtaaagct gatggttctc tgcaaaaacc ggtcgcgtat
     8221 ttgacttgta acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac tcacgacgaa
     8281 gtgatcaccc tgttccacga gttcggtcac ggcctgcacc atatgctgac ccgcatcgaa
     8341 accgctggtg tttccggtat cagcggtgtg ccgtgggatg cggtcgaact gccgagtcag
     8401 tttatggaaa actggtgctg ggagccggag gcgctggcgt ttatctctgg tcactatgaa
     8461 accggcgaac cgctgccgaa agagttgctg gataaaatgc tggcggcgaa gaactaccag
     8521 gcggcgctgt ttattctgcg tcagctggag ttcggcctgt ttgatttccg ccttcatgcc
     8581 gagttccgcc cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat caagaaactg
     8641 gttgccgtgg tgccatctcc gtcctggggc cgtttcccgc acgctttcag ccatattttc
     8701 gccggtggtt atgcggcagg ttactacagc tacctgtggg ctgacgtact ggcggcagat
     8761 gctttctcgc gctttgagga agagggcatt ttcaaccgtg aaaccgggca gtcgttcctc
     8821 gacaacattc tgagccgtgg cggttcagaa gagccgatgg atctgttcaa acgcttccgt
     8881 ggtcgtgaac cgcagctgga tgcgatgctg gagcattacg gcattaaggg ctgatcattc
     8941 agtgaaaatc tgcttaattg atgaaacagg cgccggagac ggcgccttat ctgttctggc
     9001 ggcccgctgg gggctggagc acgatgaaga caacctgatg gcgctggtgt taacgccgga
     9061 acatctggaa ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg ttgattttgt
     9121 tggcggagcg atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg cggtggcgaa
     9181 agcggtgggc attaaaggcg attatttgcc ggatgtggtg gacgccaccg cagggctggg
     9241 gcgcgatgcc tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg agcgtaatcc
     9301 agtggttgcc gcgctactcg acgacggcct ggcgcgtggt tatgcggatg cggaaatcgg
     9361 cggctggttg caggagcggt tgcagttaat tcacgcctcc agcctgacgg cgctaaccga
     9421 tattacccca cgcccgcagg tggtctatct cgatccgatg ttcccgcata agcagaaaag
     9481 cgcgctggtg aaaaaagaga tgcgtgtctt tcagtcgctg gtggggccgg atcttgatgc
     9541 cgatggatta ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg taaagcgccc
     9601 ggactacgcg ccaccgctgg cgaatgtcgc cacgccaaac gcggttgtga ctaaagggca
     9661 tcgctttgat atctatgcag gtacgccagt gtaaaagtaa agcagggtag cgataacgct
     9721 accctgcttt ctgcaagaat aattaatggc tttccggcgt cgcaatcatg cgtttcagcc
     9781 acggcaccat caacagcatc actactgcaa cgcccagcgt gaccagacca atcttaccaa
     9841 acacgttggt atagacgggc aacgtctcaa gcggatcggt aatgttgtcc ggcaccgcgg
     9901 taaatgttgc cacatagccg cccagcaaga acgcggcagc ctgcgtcagg aaccacatcc
     9961 cgagaataaa gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc aggccaaggg
    10021 cgctaataaa caattcaccc aggctctaga ataaatacac cagcacgata aaccatggcg
    10081 atgtcagccc ctggtcatcc gcaaaccaca ttcccgcagc agcagtcgtc aaaaagccca
    10141 gtgagcacat aaacatgccg agagtaaatt tcatcggcat cgagaggtct ttgcctttgt
    10201 tacccagatg cgtgtaaatg cctgccagta ttgggctggc gagtaccacc cagaacgggt
    10261 taagcgcctg gaagctgacc gggttgatgg aaaaaccgag aatttcatga tgcacgttgt
    10321 tgatggcaaa gaagttcagc gatgttggca tctgggcgta gagaatgtaa aacaccaccg
    10381 cttcgagcat caggacaaag gcgacaaaca ttttattgcg cccggtttta tccagcttga
    10441 atgcctgacg aaagaagatg atggtgacga cgatggagag aacaatcagc accagattgg
    10501 cgacttctac gttgtgcatc agccatgcac atacgaagat catcaccacg ctgccaagta
    10561 atacgtacaa cagtttgctg aagctcatcg gcttgaagtc gggttcagaa ccaatgtctt
    10621 tcaccattcc acgacaggcg atgtaaacca gtaatgcgat aattaacccc gccccgcaca
    10681 ggttgtaggt gactgaataa ccgaatctat cagcgatcac aggggccagc gataacgcta
    10741 tcaacgagcc gatattgatc gacatataga acagggtgaa tgcgccatca agccgcggat
    10801 ctttcggcgg atagcacttc gaaagcaagc tggctgggtt agctttaaac aggccgttac
    10861 cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc gacatgccgg
    10921 tcatgaagta gccaatcgcc agcacaagtg ctccaagaac aatggtgcgt ttggtcccca
    10981 acaggtggtc gccgacatag ccgccaatgg aaatgaggcc atagaccagt gcagcaaaag
    11041 caccaaaagt gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg aagaaaaccg
    11101 ccagtacgcc ctgcacgccg tagtagccga atcgctccca taactcgaca aaaaagatca
    11161 tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt tgttgtatta atatttatta
    11221 accttccaat accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg aaaaagttta
    11281 catctgtcag agattcttaa tattgatgcg gtgaatagat taatacacta ttacaacaga
    11341 aaataaccag ctgatcacac taatgatggt gttaaatttt gtattggcag gagattttga
    11401 ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta acaaggcgcg
    11461 ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga gggatgatta
    11521 ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt tgatcagctg
    11581 acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc
    11641 catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc tgccgctcag
    11701 ggtttcagtg attggatagc ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc
    11761 ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg tgtataggtc
    11821 agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc gagccataga
    11881 gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga tgagaatgtg
    11941 tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca gctagcgttt
    12001 cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa cccagccgta
    12061 aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg tcaatccgcc
    12121 ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg gttaaccttc
    12181 tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat gttggtcacc
    12241 acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg ggagcggtag
    12301 ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag caccgtcgca
    12361 ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt ctcatcacta
    12421 cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata tgttgatgga
    12481 gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc
    12541 tatgcccaac gttatcgcga tcatcacgat gatgagttta ttcgccgctg tgagcgggtg
    12601 cgtcggcagt ttattctgac tagcgcattg tgtggtctgg tggtggtcag cctgattgca
    12661 ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc gggccagtaa
    12721 actgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagaac cagtacagcc
    12781 cgccggaaag tagtaccgca gccggaaggg taaacaccca agccatcaga atactggtta
    12841 cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca gaagaggaga
    12901 gtacgtgagt ggtggaaacc ggcatcccgg tataactcgc caggccgatt gacactgccg
    12961 ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg attttctcac
    13021 cgatagtcgt tgccacacga cgccagccaa tcatcgtacc gatacctaac gccagcgcga
    13081 ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg gacttcagtt
    13141 tcttcaacag gcgctgatcg tcagcactca caccaggcat cttcaccact ttgtcgatag
    13201 tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc gacagcttgt
    13261 cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg ttaatggtat
    13321 tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga gccggtacta
    13381 actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag taagcttcga
    13441 cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc atgttcacca
    13501 cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg cctttctgac
    13561 catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc gcgatacgcg
    13621 tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg gtcaggtgga
    13681 tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc agaccgccag
    13741 caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt aatactttcg
    13801 ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt aaaccaatcc
    13861 cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag taccaggtac
    13921 ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg ccatgagacg
    13981 atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag gcaacactca
    14041 gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg gcgagctgag
    14101 aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca tggaaaccat
    14161 tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc ccggtatgca
    14221 aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg acatgaacgc
    14281 ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt tttttgattt
    14341 gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg caaatacttc
    14401 cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc acgcccgttt
    14461 tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg ctggtgcggc
    14521 gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc tgatcgataa
    14581 tggtaaaata ctagggcgca aaatccttat gtctggcggt gggcgctgca actttaccaa
    14641 cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta agtctgcact
    14701 cgcacgtttt acccagtggg atttcattga tctggtcaat aaacacggta tcgcctggca
    14761 cgagaaaacg ttagggcaac tcttctgcga tgactccgcg cagcagattg tcgacatgct
    14821 ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag tgctgagtgt
    14881 ggcgaaggat gaaacaggct tcacgcttga tctgaacggc atgactgtcg gttgcgaaaa
    14941 gctggtcatc gcgaccggtg ggctgtcaat gccggtgctg ggcgcatcgc cgtttggtta
    15001 taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc tggtgccatt
    15061 cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg cggtgccttc
    15121 cgtgattacc actgaaaacg gcaccgtttt ccgtgagaac ttactcttca cccaccgcgg
    15181 cttatctgga ccggcggtgt tgcagatttc aagctactgg caaccgggtg aatttgtcag
    15241 tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc gtaacgcaca
    15301 tccgaatcaa agcctgaaaa acacactggc ggttcatcta ccgaagcggt tggttgaacg
    15361 cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg tgtgtgacca
    15421 acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca ctgaaggcta
    15481 tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactttctt cacggacgat
    15541 ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg ttaccggctg
    15601 gctggggggc tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg cgcaggattt
    15661 ggcgtcaatt gtgaaataac catgacgttt tcccgtcagc cgtttgtata tgtcgttatt
    15721 aagtcatttt ggtacggttc tgcatccgac attctggtgt taactcgctg ttttgttagc
    15781 gaattaacgc tattgccgtt gtggttattt tatagtg
//
LOCUS       DAJTRQ010000089        15658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000089 DAJTRQ010000000
VERSION     DAJTRQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..140)
                     /locus_tag="OO332_002838"
     CDS             complement(<1..140)
                     /locus_tag="OO332_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5943252.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEA"
     gene            complement(137..2821)
                     /locus_tag="OO332_002839"
     CDS             complement(137..2821)
                     /locus_tag="OO332_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5943253.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(2913..3413)
                     /locus_tag="OO332_002840"
     CDS             complement(2913..3413)
                     /locus_tag="OO332_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5943254.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3443..4159)
                     /locus_tag="OO332_002841"
     CDS             complement(3443..4159)
                     /locus_tag="OO332_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5943255.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4455..5231
                     /gene="exuR"
                     /locus_tag="OO332_002842"
     CDS             4455..5231
                     /gene="exuR"
                     /locus_tag="OO332_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5943256.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5576..6238
                     /locus_tag="OO332_002843"
     CDS             5576..6238
                     /locus_tag="OO332_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5943257.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6242..6625
                     /gene="mzrA"
                     /locus_tag="OO332_002844"
     CDS             6242..6625
                     /gene="mzrA"
                     /locus_tag="OO332_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5943258.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            6772..7126
                     /locus_tag="OO332_002845"
                     /pseudo
     CDS             6772..7126
                     /locus_tag="OO332_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7164..7469
                     /locus_tag="OO332_002846"
     CDS             7164..7469
                     /locus_tag="OO332_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5943259.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7472..7876
                     /locus_tag="OO332_002847"
     CDS             7472..7876
                     /locus_tag="OO332_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5943260.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            7866..8165
                     /locus_tag="OO332_002848"
     CDS             7866..8165
                     /locus_tag="OO332_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5943261.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8351..8743
                     /locus_tag="OO332_002849"
     CDS             8351..8743
                     /locus_tag="OO332_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5943262.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            8813..9799
                     /gene="yqjG"
                     /locus_tag="OO332_002850"
     CDS             8813..9799
                     /gene="yqjG"
                     /locus_tag="OO332_002850"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5943263.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10092..10460
                     /locus_tag="OO332_002851"
     CDS             10092..10460
                     /locus_tag="OO332_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5943264.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10667..11563)
                     /gene="yhaJ"
                     /locus_tag="OO332_002852"
     CDS             complement(10667..11563)
                     /gene="yhaJ"
                     /locus_tag="OO332_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5943265.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDHSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11668..12369
                     /locus_tag="OO332_002853"
     CDS             11668..12369
                     /locus_tag="OO332_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5943266.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12392..12556
                     /locus_tag="OO332_002854"
     CDS             12392..12556
                     /locus_tag="OO332_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943267.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12768..14060)
                     /locus_tag="OO332_002855"
     CDS             complement(12768..14060)
                     /locus_tag="OO332_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5943268.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14088..15419)
                     /locus_tag="OO332_002856"
     CDS             complement(14088..15419)
                     /locus_tag="OO332_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5943269.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4085 a         3981 c         3637 g         3955 t
ORIGIN      
        1 gcttcatcct tccagaagta ataatccgct ctaccagatg ccaaatcata cgtaatttca
       61 ctggtcacgt caggagctgc ctgaacgcca gtgagcgtgc caaacaagcc caatatcgcc
      121 gcaatcaatc gatttctcat ggtgcaacct ccctttgcgc cgtctgaacg tttccacgta
      181 atttaggctg tttcaactgt gcctgaacct gaatcgactc cggtaattgt gaataagtca
      241 ccacactaca agtaacatct ccgctgtaac gaatgctggc gcgtttcttt acaacactca
      301 gtggacactt aaggaattgc tgcccggaaa gcagatagag ctctttttcg ctgccgttaa
      361 tttcaagtgt aaatccgcca tcttcagcta ccagtaacat ggcgctgttc agccccacaa
      421 tcgggaattt tttcaccgct gccggcagta ataaacgacc gatatagttg tagttaaagc
      481 tggcagacac ttcgcgatag cgcagcttgc caggcaacaa cattaattca cgattccctg
      541 caccgttaat gatgtttgtt gtcccctgct gattgccctg gctactgtcg ttaacctcaa
      601 caaaaccaga ctgaaatcca ggataaggga acaacacact gtcactttga ccaagctgca
      661 aagttcggct gcctgccacc cgcgcttcga gttgcagatc ctgactctct tcatcctgtt
      721 cagagaagcc tttcacatcc accagtaccg cacccagcaa atcgtctgta ccactggctc
      781 ccaaattaac gccatagcga ctgacagcca gcgtagagct ataagtgccc gtaaaggccg
      841 aaagatgatc atgattctta cggtcataac tatcagatac ggtagcgtag acatttccgt
      901 attgcccatc ccaacgcccg ttaaccgcgc tgtttatggt atcggtgttc aggcctccga
      961 cggttacgcc aagttcttta tggctgaatg aatcgttata aaaggtatgc gataactgcc
     1021 atgacgtttg atcgccatcc tgttcgctat aacgataatc cgtagacaca ttggttgaat
     1081 ggctgttgtt attgctatcc atcgttggcg tgtcagataa cgaaaacgat aagtacaagc
     1141 cgttgtcgtc gttatcgcca ccgttgtcat tacgatggaa aacaccgact gacgtactca
     1201 aaatccagtc acgcatcaca atagcgcggg aagcgctaag ttgtacagtt tcagagatac
     1261 tttgcgctgt tgtctggcga agatagttat tttcaaagct ctgctgttta tcccaacgca
     1321 aactatcatc agtgttattt tgtgtgcagg tataacccag catcatattc cagccgacaa
     1381 aattcagtga aactgtcgcg gaaatacttt cataacagct taaggcgtta tagctctctc
     1441 tgctgtcggt tgtacaagca tcgccgtcag agttggtccg ataaaagccc aacgtcggcc
     1501 atcccggatt actccagttg gcctgttgca tatcaccttt gccgccgtca tcgttacgga
     1561 acacgctggc gctgattgca agattcccca ccccgcctaa atctgccgtc cagttattac
     1621 ctaactcgaa agcactcaca tcatcggcat tcgccagccc tgcgtagagc tcatattgcg
     1681 gatgtaatgg caggcgtact cctagctgat aagctgaact ttcatcatca gaaacctgtg
     1741 atgtagtttt acctgcctgt aagaaccatt gcgcatttcc gtcagtcaga ccgccggttt
     1801 tggtaaacgg cacaagctcg gtgcgggtga gttggttatt ttcgtagact tttaatgcca
     1861 cgctgtagct gcctggcgga aaggaactgg tatcaataaa ttgcgaacca ctattgagat
     1921 aaaacgatcc caaaagttgc tcattacgat aagcgtcaac acgagaattg cgcgaaagca
     1981 gaaccattac tggggttcct tgctggcttt gcgcctggtt taaatagctc agtgtcgacc
     2041 cgatacgcat cccatcgatt gcaccgagtg gcagaaagtt aaaggtaaag ttcccgcctt
     2101 gtgcattaaa tagtgtgcgg ttgtccatgc gacccgcctg cacataataa cgacgtaaaa
     2161 aatcataacg ataatagaga tcgctgacgt cagtattact gtcactgaca tcatctgcat
     2221 cataaccgtt gaaattccag tgtgcaccaa tatagctatt ttctgttatt cccagcgcac
     2281 cgtttccctg aatagacaac gattggtaat catcctgtgc cagaacattg atgtcttgct
     2341 gatgtataaa agcgttaaca gtatttttgt ctggatttaa atataatgac ttcgcatcga
     2401 aagctgaatt ccattgcgga ttaataaaca acgttgccga actttcttca tcattataga
     2461 ttatttccag cgtatcggta ttcagaaaac cacaccctga gtcagtacgt acataaccgc
     2521 aagccaattc accgtggcga gctagcggct gcgataattt ttctttcact atatgcgcga
     2581 ttttctgatc gtctacatcc agttccagct tgttatatag acttgctgga tcaagaaatg
     2641 ttacggtatc aagattaacc attgcataat atttaccgag acttttccct gaaaaataaa
     2701 cctcgatata ctcctgctgt ccctgagcaa taacctcaaa ccctgcggga attttggtta
     2761 atgtcgcctc tgccgggctg agactcgcca ggatcaaaag agccagtaat tttttatcca
     2821 tggcaatcct gaaatccata acaaatacta aaacagttaa aataagggag ggctacccct
     2881 ccctttgtta cgtgttaatt ctgttatccg gattaaatat cctgagacag ataaatgctt
     2941 actacaccac ggtagatgcc tgtttccaga acacctttgg tggtttgagc aaacatcagg
     3001 tttttagcca gagaaccgtt agttaacgca tcggaagcaa agattttggt tgcggtaaaa
     3061 gtggttgccg catcagcttt aatttcttca ccaccccagg ttactgtcag cggaacaatc
     3121 ttgctagcat ccaggctttg aaccagttgc gctggggaca ctaactgcat ttttacatct
     3181 ttggtaacgt cgttagacca gattttggtc atcaactggt aagattgcag accctgcccc
     3241 ggcagatatt gcatttctac tgctttcggc aacgcggtgt tatcggtctg tgtcatatcc
     3301 agagcggcat caacgttggc cgttacagta atatcctttt gtactgccaa tgcggagcct
     3361 gcaacagaaa acatcgctgc gaccagaaga gattttgcaa ataccttttt cattactatt
     3421 ccttcgttta ttacaatgat aattaatgga ccgccagttc aaactcgcca ttatctttgg
     3481 tgatccagtt attatatttg acaacgattt tgtcgtagcc agatatgctt tcaagatttc
     3541 tttcctgacc agggaatatg tttttattta cggtctgtat cttacagttt tctttgctat
     3601 tatttttaca ataagcgatc gttaaaagct ttaagcgttg attccctgca ttaattaatt
     3661 tttgggcaga atttacttct aacttagcta taggttgctg aggtggaaca ctaacaacga
     3721 ttccccaaat taagttaact gttaactgca tagagagatc ttttttatct gtggcaacgt
     3781 catcaatact gggaaccgct tcaaatttaa cgcgataatt tttctctttt tgctctggtt
     3841 ccatcgcaac aaaacgtacg gttttgctac taccggctgg taaagcgaat ttaggtggca
     3901 taacaaccag atgatttgca tcaccggatt taatctcaac ttcattttcc tgaggcgtcg
     3961 atggattatc gatacggaat actgtcgcct tgatgtactg aacttcatta cttttagata
     4021 ttacgcgaac gttgccttca ccctgactat taatggaaac aggcatagga taaacagtca
     4081 tatttgactg tgccaaaaaa ggagcgaaca cgaataaaag cggcgtaagc cgtaataaga
     4141 gattaatttt cgccaccata taattttgat cttacaatat cgtttcaaaa aagttggtgt
     4201 attagagatg tagatttatt gtaagtcaat ataaaaaagc atttttagtt aacattaaat
     4261 ttaaaggctg ggaattaact tactgaaatt tagtagcttt cacaatgacg atacttattt
     4321 tttaggaatt tataaatatt cacaatatta atttaataca ttaattcaat catcaaattt
     4381 atatatgacc aatggcatac ccatgaataa agtggtatga taacttcgtc cgcattccac
     4441 tccgggagac gcatatggaa atcactgaac cacgccgttt gtatcaacaa cttgctgctg
     4501 acctgaaaga gcgcattgaa cagggcgtct atctggtggg tgataaactg cctgccgaac
     4561 gctttattgc cgatgaaaag aacgtcagcc gaacggtagt tcgtgaagcc atcatcatgc
     4621 tggaagttga aggctatgtg gaggtgcgta aaggttcagg tattcatgtg gtttccaacc
     4681 agccgcgtca tcagcaggcg gctgacaata atatggaatt cgccaattac ggtccgtttg
     4741 agttgcttca ggctcgccag ctcatcgaaa gtaatattgc cgagttcgcg gcaactcagg
     4801 taacgaaaca agacatcatg aaactgatgg ccattcagga acaggcgcgc ggcgaacaat
     4861 gcttccgtga ttccgagtgg gatttacagt tccacattca ggtagccctg gcgacgcaga
     4921 actccgccct ggcggccatc gttgaaaaaa tgtggaccca gcgtagccat aacccgtact
     4981 ggaaaaaact acacgaacac attgattccc gtaccgttga taactggtgt gatgaccacg
     5041 atcaaattct caaggcgtta attcgcaaag atcctcatgc tgctaagctg gcaatgtggc
     5101 agcatctgga aaacaccaaa atcatgttat ttaacgaaac cagcgacgat ttcgagttca
     5161 atgccgaccg ctatctgttc gccgaaaacc cggtagtgca tctcgatacg gccaccagcg
     5221 gcagcaaatg aaattcctgt ccgacaggcg ctgcaatgcg cctgtttgtc tcgtcggtaa
     5281 gcgaaaagta taaagtgtca gcctgtgtaa atcctctcgc caccctcccc tgcaatcagc
     5341 aaaatcagac tccacggaca tgtaattttg ataacgaaca acgttgacct ttgttacaat
     5401 tagattcaat ttgaatttat gcttttgaat gctttcttat ctcacgtttt aacagggaat
     5461 agttcaggct gtgttgatgt atcaaacccg cagaacatac caaaacagca ataacattgc
     5521 ggtagtgcat cttttaaaac cagcgtggcg ttaaccgatt caccaggaat aatgaatgga
     5581 acttttgacc caattgctgc aagccctgtg ggcgcaggat tttgaaaccc tggccaatcc
     5641 atcgatgatt ggcatgttgt attttgtctt gtttgtaatt ttgttccttg aaaatggctt
     5701 gcttccggcg gcctttttac cgggcgacag tttactggta ttggtcggcg tgttgattgc
     5761 gaaaggcgcg atgggttacc cgcaaacgat tctgctgctg accgttgccg ccagcctcgg
     5821 ttgctgggtc agctatattc aggggcgatg gctgggcaat acccgcaccg tacaaaactg
     5881 gctatctcat ttacccgcgc attatcatca acgcgcacac catctttttc ataaacacgg
     5941 tttatcggcg ctgttaattg gtcgctttat tgcatttgtc agaacactgc tgccgacgat
     6001 tgccgggtta tcagggctga ataacgcgcg ctttcagttc ttcaactgga tgagcggtct
     6061 gctgtgggta ttgatcctga caactctggg ttacatgctc ggcaaaacgc cggtattttt
     6121 aaagtacgag gaccagctga tgtcatgcct gatgctgctc ccggtggtgc tgctggtgtt
     6181 tggcctggca ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc gggggtaagg
     6241 gatgcaaata cctcgcatgt cgcttcgcca gctagcctgg tccggcactg ttttacttct
     6301 ggtcggcacg ctgttgctgg cctggtccgc ggttcgccag caagagtcta cgctggcgat
     6361 tcgtgccgtt catcaaggca caacgatgcc agacggtttt tcgatctggc atcaccttga
     6421 cgctcatggc attcctttca aaagtatcac ccccaaaaac gacactctgt taattacatt
     6481 tgactccagc gaccagagcg ccgccgcaaa agcggtcctc gacagaacat tgccccatgg
     6541 ctacatcatt gcgcagcagg acaataacag tcaggctatg cagtggctga cccggttacg
     6601 ggataactct catcgcttcg gataacttcc tgttatccga aacatatcac tcactttggt
     6661 gatttcaccg taactgtcta tgattaatga ggcggtggtt aacactaccg cttcgttgtc
     6721 tggacaatcg ttcctttgta ataggtccga atcacaatgg aaggttcaag aatgaaatac
     6781 cgcatcgctt tagctgtttc tctctttgct cttagtgccg gtagttatgc cactaccctg
     6841 tgtcaggaaa aggagcaaaa tatccttaag gagatcagct atgccgaaaa acaccaaaac
     6901 cagaatcgta ttgacggtct gaataaagcc ctgagtgaag tccgggccaa ctgttcagat
     6961 agccagctgc gtgccgatca tcacaagaaa atcgcaaagc agaaagatga ggtggcggaa
     7021 cgccagcaag atttagccga ggcgaagcaa aaaggcgatg ccgatagaac ggaaactggc
     7081 agaagcgcag gaagagctga aaaagctgga agcgcgcgac tactaactca caatagtcac
     7141 tacttactca cctggagaaa actatgtcga aagaacacac tacggaacat ctgcgtgctg
     7201 agttgaaatc cctttccgat acgctggaag aggtgcttag ctcatctggc gagaagtcga
     7261 aagaagagtt gagtaagatt cgtagcaaag cggagcaggc actgaaacag agccgttatc
     7321 gcctgggtga aaccggtgat gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg
     7381 atgagtatgt gcgtgaaaat ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag
     7441 tgctcggcgt tctgctgtcg cgtcgttaat tatggcggac actcatcacg cgcaagggcc
     7501 cggtaaaagc gttctgggca tcgggcagcg aattgtttcc atcatggttg aaatggtgga
     7561 aacacgtctg cggctggcgg tggtggagct ggaagaggaa aaagctaatc tctttcaact
     7621 tttactgatg ctgggcctga cgatgctttt cgctgcattt ggtcttatga gcctgatggt
     7681 gctaattatt tgggcggttg atccgcaata tcgcctgaat gcgatgattg ccaccaccgt
     7741 ggtgttgctg ctactggcac tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc
     7801 caccttgctg cgccatacac gccatgagct ggcaaacgat cggcaactgc tcgaggagga
     7861 gtcccgtgag cagtaaagtc gaacgtgaac gacgtaaggc gcaactgctt agccagatcc
     7921 agcaacaacg gctggatctt tccgccagtc gtcgtgaatg gctggaggca acaggcgctt
     7981 acgatcgtcg ctggaatatg ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg
     8041 tgatggcgat ctggacgatt cgccatccta atatgctggt ccgctgggcc agacgcggtt
     8101 ttggcgtatg gagcgcctgg cgtctggtta aaacgaccct caagcagcaa cagcttcgcg
     8161 gttaacttcc ctctggccgg agcaattccg gccttatccc tcaaattttt tgaatatttt
     8221 tgacagtttt ccttgctaac aatcatcatt caccacgttt atgattctct ccatcgacag
     8281 caacgacgct aataccgcgc ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt
     8341 ggagagtatg atgaaaaaat tagaagatgt tggtgtactg gtagcgcaca ttttaatgcc
     8401 gattctgttt attaccgcag gctggggaaa aatcaccgct tactctggta cccaacaata
     8461 tatggaagca atgggtgtcc cgggttttat gctgccgctg gtgattctgc ttgagttcgg
     8521 tggtggtctg gcaatcctat tcggtttcct gactcgcacc acaaccctgt ttactgcggg
     8581 ctttacgctg ctgacggcat ttttatttca cagcaacttt gctgaaggcg tcaactcgct
     8641 gatgttcatg aaaaacctga caatttctgg cggattcctg ctgctggcaa ttaccggtcc
     8701 gggcgcgtat agcatcgacc gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa
     8761 cgataaaaaa cgaggaggaa gctcctcgtt tttgctattg gaggagagaa aaatgggtca
     8821 actgattgac ggcgtctggc atgacacctg gtacgatacc aaatctaccg gcggtaaatt
     8881 tcaacgttca gcttccgcat ttcgtaactg gctcactgcc gatggcgcac ctggccccac
     8941 tggcacaggc ggttttatcg cagagaaaga tcgttatcat ctctatgttt cactcgcctg
     9001 cccgtgggcg caccgcacgc tgatcatgcg caaactcaaa ggactggaac cgtttatttc
     9061 cgtttccgta gtgaacccgc tgatgctgga aaacggctgg acctttgatg acagttttcc
     9121 gggagcaacc ggagatacgc tctatcaaca tgaatttctg tatcagcttt atctccacgc
     9181 cgatccacac tacagcggac gagttactgt tcccgtgctg tgggacaaaa agaaccacac
     9241 catcgtcagc aacgaatcag cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct
     9301 gggcgcaaaa gcgggtgatt actacccacc agccctgcaa acgaaaattg acgaacttaa
     9361 cggctggatt tatgacaccg ttaacaacgg cgtgtataaa gctggttttg ccaccagcca
     9421 gcaagcctac gacgaggcgg tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat
     9481 tttaggccag catcgttacc tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg
     9541 gactacgctg gtgcgttttg atccagtgta tgtgacccac ttcaagtgtg ataagcaccg
     9601 catcagcgat tacctgaatc tgtatggctt cctgcgcgat atctaccaga tgccggggat
     9661 cgccgaaact gtcaatttcg atcacatccg taatcattac ttccgcagcc ataagaccat
     9721 caaccctacg gggattattt caattggtcc gtggcaggat ctcgatgaac cgcatggacg
     9781 agatgttcgc ttcggttaaa aaaaggcacc ttacgaggtg cctctgataa ttagctaagt
     9841 gcatgttgtt catgtcggat gcggcgtgaa cgccttatca aacctacaaa gcgtttatat
     9901 tcaatatgtt acaatgaccg tgtaggccag ataagcgtag cgcatctggc gattttgcgc
     9961 ttgccatcag tcttagcgcg ccttcacggc actttattaa ttccttgaac gaatatttac
    10021 tgccatttca ttcacgtcta ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa
    10081 ttgaggcaaa aatggactgg tatctgaaag tactaaaaaa ttatgtcggt ttccggggtc
    10141 gtgcgcggcg caaagagtac tggatgttta ttctggtcaa catcatcttt acgttcgtgc
    10201 tggggctgct ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc atcctgacga
    10261 caatctacgg tattctggtg tttttaccgt ggtgggcggt tcagttccgc cgcctgcacg
    10321 acaccgaccg ctcggcgtgg tgggcactgc tattcttaat cccgtttatc ggctggctta
    10381 ttatcatcgt ctttaactgc caggcgggta cgccaggcga aaaccgcttt ggaccagatc
    10441 caaaactgga acaagaataa tgattaagca ccttgtaaaa ggtgcttcaa attgttgaca
    10501 aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccagccta caaattgcca
    10561 aaattcaata tattgcagtg actgcgtagg cctgataagc gtagcgcatc aggcagtttt
    10621 gcgtttgtca gcagtcagaa gcaccttgtg aaaggtgctt aatagattat tttccagcga
    10681 acaactttgg aatttcccgc agacaccagg atttcgcttc ccccatactg tcacgacgcc
    10741 aggccataat aatatcgatc tcgctggtcg attccgggct gacgacacgc aaccgccctt
    10801 ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta
    10861 atgcctgacg tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagct
    10921 gtacggtcaa caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca
    10981 cacgcgtcac ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa
    11041 cgtagacgtt cattaacgta tagagcttgc gcgagttgat ctccgacgag gaacgaaaat
    11101 gcatatccgg cgcgataacg atatccgccc gcccctgctc cagccgttcc cacgcccccg
    11161 ccagcacttc tgtgatgatt gccagttggg tattggcttt tgccgccagt ttgtcgatta
    11221 acgggaaaaa ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc
    11281 aaccgcgcgc gagagcttcc gcatcagtag tcagtttatc tgcggcttcc agcaaaacgc
    11341 gccctcgctc cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagtggt
    11401 caaacagcac cacatccagc tcttcttcca gtttttgcat gatgtagcta agtgcggaag
    11461 gcacgcgtcc cagctcatcc gctgccgccg caaaactgcc ccggcgatcg atcgcatcca
    11521 taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga
    11581 aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata
    11641 aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga
    11701 caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac
    11761 ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt
    11821 gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga
    11881 gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg agaggcgttg
    11941 ctgctctcta cccagccggg tgtcagctat agcgaacaca atctcagcaa agacaaaccg
    12001 ttaacgcgaa tgcagctttg gctggatacc tgcccgcagc gagagaatcc gctgattcaa
    12061 aagctggcgc ttaatatgga caagcagcaa ttaatcgcct cgccagaggg tgcgatggga
    12121 agcctgcaat tacgccaaca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt
    12181 gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat
    12241 gcgcttacgc atcatgaaga gaaagcggcg ctaacctgcg gtgatggggc gtttattcgt
    12301 gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg
    12361 cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcgacca aaaagcgcca
    12421 gctagcgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga
    12481 agagatgttg agcgagctgg aagctatcgt cgcggatgct gaaacgcgtt tagccgagga
    12541 tgaagctacc gcgtaaagac aggttgccgg atgcggcgta aacgccttat ccggcctaca
    12601 aaacacaaca acatcaacaa attaaacacc ccgtaggccg gataagatgc gccagcatcg
    12661 catccggcat caggtacaaa acacaacaac atcaacaagt tgagcacccc gtaggccgga
    12721 taaggtgcgc cagcatcgca tccggcacaa tactcaaaac ctggcgttta tctggccttg
    12781 ctcgccataa tctcgataat ctgccgatcc gtttgctgca tcgaatggct tgctaacgca
    12841 cacaggttgg caatcgactg ctcaacatca tgcgccacga tcccttcatt gccggtcacg
    12901 gcggtatcat ccagcgccat taacaccgct ttccacgcag ccgaagcact ggtcgaaacc
    12961 ttcgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag
    13021 atagtttcat aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg
    13081 gtcgttgcgg cacaaagtgc agacaaacgc ggtaactggt tatggatgta aattgcgctc
    13141 aaatgagaaa gcatcagcgc acgcgccagt cgttcatcat ccgctccgaa gtgttctgct
    13201 acaaccacca caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata
    13261 gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg
    13321 gatgaagaga gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca
    13381 atatgcagcc cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg
    13441 gaatcgagaa taaagcggat cgccgcaaat gggacttcat tgacgaactt caggatctca
    13501 gccagcgtcg ttctggaaag caccgtcagc ggcgactctt gctcgccctc tgccacacac
    13561 gcctgctggg taaacaccac accattgtgc gtctcaatat gcacaatgtt ggtatgcccg
    13621 ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg
    13681 atttcattgc aaggttcctg gatcttaaca gagactttcc ccgccgccag cagtgctttg
    13741 gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttggcattt
    13801 ccacctaacg cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg
    13861 gtgatgccca gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc
    13921 ggaccttcca gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta
    13981 catcccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga
    14041 tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc
    14101 aggaacagag aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct
    14161 ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc
    14221 atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg
    14281 gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta
    14341 atcttctcgg caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg
    14401 cgaaagccta aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg
    14461 ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc
    14521 gcggaaatat tctgctcgta ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac
    14581 gacacggcgt agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt cattgcccgc
    14641 aatgctttat gccgcgccac ttcaattgat ttttcccgcg agcgataaga gatcaccatc
    14701 gggcttaacg tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg
    14761 tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc
    14821 accatcgaca cgcccagcgc agcgactacc agcagcttgg tcagcaccat gtcggtcgaa
    14881 attttgaata acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc
    14941 agaccataaa agggactgtc tgacagcaac ccttccgtca cgccgaaggt atgcagatag
    15001 gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg
    15061 aaatagagcg cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc
    15121 gggtaatctt tacattctgg tgattctgcc agcgtattaa taaacaaccg ctgaaacaga
    15181 tacattgccg ggtaaccaat caccgatgag agcaaaaata cccacaatcc catcaaaccg
    15241 acctgcaccg ggagaaaaac aatccccgcg ccaatcgcca tcccgatact cataatcacc
    15301 cagcaggtgt cagtactgtc gaatttgatc gcttctcgcc actcgctctc actcattcca
    15361 gcacgacccg ccggggtcga agcgtctgca atgacgcctt tattcgatgc aatttccata
    15421 atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat caaggcgata
    15481 tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc gcgaggtcag
    15541 agaaaatctt cacaatcaag actttaaaat aacagagatg gataagaatt ttctactaaa
    15601 ttaatcgcaa gagaatattc attctctatt tgcggcagat cacaaaaaaa aggtgcac
//
LOCUS       DAJTRQ010000090        15601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000090 DAJTRQ010000000
VERSION     DAJTRQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..96
                     /locus_tag="OO332_002857"
     CDS             <1..96
                     /locus_tag="OO332_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943270.1"
                     /translation="IHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            279..473
                     /gene="bfd"
                     /locus_tag="OO332_002858"
     CDS             279..473
                     /gene="bfd"
                     /locus_tag="OO332_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5943271.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            546..1022
                     /gene="bfr"
                     /locus_tag="OO332_002859"
     CDS             546..1022
                     /gene="bfr"
                     /locus_tag="OO332_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5943272.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1019..1486)
                     /locus_tag="OO332_002860"
     CDS             complement(1019..1486)
                     /locus_tag="OO332_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5943273.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1865..2176
                     /gene="rpsJ"
                     /locus_tag="OO332_002861"
     CDS             1865..2176
                     /gene="rpsJ"
                     /locus_tag="OO332_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5943274.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2209..2838
                     /gene="rplC"
                     /locus_tag="OO332_002862"
     CDS             2209..2838
                     /gene="rplC"
                     /locus_tag="OO332_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5943275.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2849..3454
                     /gene="rplD"
                     /locus_tag="OO332_002863"
     CDS             2849..3454
                     /gene="rplD"
                     /locus_tag="OO332_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5943276.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3451..3753
                     /gene="rplW"
                     /locus_tag="OO332_002864"
     CDS             3451..3753
                     /gene="rplW"
                     /locus_tag="OO332_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5943277.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3771..4592
                     /gene="rplB"
                     /locus_tag="OO332_002865"
     CDS             3771..4592
                     /gene="rplB"
                     /locus_tag="OO332_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5943278.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4609..4887
                     /gene="rpsS"
                     /locus_tag="OO332_002866"
     CDS             4609..4887
                     /gene="rpsS"
                     /locus_tag="OO332_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5943279.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4902..5234
                     /gene="rplV"
                     /locus_tag="OO332_002867"
     CDS             4902..5234
                     /gene="rplV"
                     /locus_tag="OO332_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5943280.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5252..5953
                     /gene="rpsC"
                     /locus_tag="OO332_002868"
     CDS             5252..5953
                     /gene="rpsC"
                     /locus_tag="OO332_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5943281.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5966..6376
                     /gene="rplP"
                     /locus_tag="OO332_002869"
     CDS             5966..6376
                     /gene="rplP"
                     /locus_tag="OO332_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5943282.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6376..6567
                     /gene="rpmC"
                     /locus_tag="OO332_002870"
     CDS             6376..6567
                     /gene="rpmC"
                     /locus_tag="OO332_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5943283.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6567..6821
                     /gene="rpsQ"
                     /locus_tag="OO332_002871"
     CDS             6567..6821
                     /gene="rpsQ"
                     /locus_tag="OO332_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5943284.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6986..7357
                     /gene="rplN"
                     /locus_tag="OO332_002872"
     CDS             6986..7357
                     /gene="rplN"
                     /locus_tag="OO332_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5943285.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7368..7682
                     /gene="rplX"
                     /locus_tag="OO332_002873"
     CDS             7368..7682
                     /gene="rplX"
                     /locus_tag="OO332_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5943286.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7697..8236
                     /gene="rplE"
                     /locus_tag="OO332_002874"
     CDS             7697..8236
                     /gene="rplE"
                     /locus_tag="OO332_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5943287.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8251..8556
                     /gene="rpsN"
                     /locus_tag="OO332_002875"
     CDS             8251..8556
                     /gene="rpsN"
                     /locus_tag="OO332_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5943288.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8590..8982
                     /gene="rpsH"
                     /locus_tag="OO332_002876"
     CDS             8590..8982
                     /gene="rpsH"
                     /locus_tag="OO332_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5943289.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8995..9528
                     /gene="rplF"
                     /locus_tag="OO332_002877"
     CDS             8995..9528
                     /gene="rplF"
                     /locus_tag="OO332_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5943290.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9538..9891
                     /gene="rplR"
                     /locus_tag="OO332_002878"
     CDS             9538..9891
                     /gene="rplR"
                     /locus_tag="OO332_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5943291.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9906..10409
                     /gene="rpsE"
                     /locus_tag="OO332_002879"
     CDS             9906..10409
                     /gene="rpsE"
                     /locus_tag="OO332_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5943292.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10413..10592
                     /gene="rpmD"
                     /locus_tag="OO332_002880"
     CDS             10413..10592
                     /gene="rpmD"
                     /locus_tag="OO332_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5943293.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10596..11030
                     /gene="rplO"
                     /locus_tag="OO332_002881"
     CDS             10596..11030
                     /gene="rplO"
                     /locus_tag="OO332_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5943294.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11038..12369
                     /gene="secY"
                     /locus_tag="OO332_002882"
     CDS             11038..12369
                     /gene="secY"
                     /locus_tag="OO332_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5943295.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12401..12517
                     /gene="rpmJ"
                     /locus_tag="OO332_002883"
     CDS             12401..12517
                     /gene="rpmJ"
                     /locus_tag="OO332_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5943296.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12664..13020
                     /gene="rpsM"
                     /locus_tag="OO332_002884"
     CDS             12664..13020
                     /gene="rpsM"
                     /locus_tag="OO332_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5943297.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13037..13426
                     /gene="rpsK"
                     /locus_tag="OO332_002885"
     CDS             13037..13426
                     /gene="rpsK"
                     /locus_tag="OO332_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5943298.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13460..14080
                     /gene="rpsD"
                     /locus_tag="OO332_002886"
     CDS             13460..14080
                     /gene="rpsD"
                     /locus_tag="OO332_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5943299.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14106..15095
                     /gene="rpoA"
                     /locus_tag="OO332_002887"
     CDS             14106..15095
                     /gene="rpoA"
                     /locus_tag="OO332_002887"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5943300.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15136..15519
                     /gene="rplQ"
                     /locus_tag="OO332_002888"
     CDS             15136..15519
                     /gene="rplQ"
                     /locus_tag="OO332_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5943301.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4014 a         3670 c         4159 g         3758 t
ORIGIN      
        1 atccacccga tcgcgatgga cgacggtctg cgtttcgcaa tccgtgaagg cggccgtacc
       61 gttggcgcgg gcgttgttgc taaagttctg ggctaattac acgttaatta gttttgaatt
      121 gaaaagggcg cttcggcgcc ctttttgctg cccgtaattt gagctcgaaa tatttagtcg
      181 taattttgct cgctgaacta tggcgcaaat aaaactgtgc tattgtaatc ataaccattc
      241 tcatttacac tttgtgcaga atttgaacgg gagcaatcat gtacgtttgt ttgtgtaatg
      301 gtgtaagtga caaaaaaatt cgccaggcag tccgtcagtt tcatccgcaa tctttccaac
      361 aattgcgtaa gtttattcct gtgggaaatc aatgcggtaa gtgcattcgc gccgcgcgcg
      421 aagtgatgca ggacgagttg atgcagatgc cagaatttaa agagattgcc tgaagcccac
      481 gcttattttt tgacatcccg ctagccccat ctacgctcta atagtggaag cggagggact
      541 ataaaatgaa aggtgatgtt aaaatcataa attatctcaa taaactattg ggaaatgagc
      601 ttgtcgcaat caatcagtat tttctccatg cccggatgtt taaaaactgg ggcctgatgc
      661 gtctcaatga cgtcgaatac catgaatcca tcgatgagat gaaacacgct gataaatata
      721 tcgagcgtat tttgttttta gaaggaatcc ccaacttaca ggatttaggc aaactgggga
      781 ttggtgaaga tgtcgaggag atgctgcaat ctgacctgcg attagaactg gaaggcgcga
      841 aggatctgcg tgaggctatc gcatatgccg acagcgttca tgactatgtt agccgcgata
      901 tgatgatcga gatccttgct gatgaagaag gtcatatcga ctggctcgaa actgaactgg
      961 atctcatcgg taaaatcggc ctacaaaatt acctgcaatc acaaattaaa gttaaagatt
     1021 aacggcctat cagcaaacta tcccagccta taacgaaacc cgcagccgcc agaaatggcc
     1081 caaaaggcag cgggtttttt aatgattctt ttcctctcat gagcagacca ataactacgg
     1141 ctccgcaagc gaatgaggca gcaaggaaaa ccagtcgtgg taagaaggcc catgaatgcc
     1201 aggcgccaag ggcggcgaga aacttcacat caccatagcc taatccttct ttatgtcgca
     1261 gtatgcgata cccccagtaa atgacagcga atgtgccgta accaacaatt gccccccata
     1321 acgcatcggc taaaccatca ggatgacaaa cttgatagaa caaaagacct gaccagagta
     1381 acgggcacgt aaatctgtca ggtaacaagc catgctttgc atcccagaaa aaaagtaggg
     1441 ctgataaaca agcgtacaaa attagaaagg gaagggtagt gtccataaaa catcctctgt
     1501 aattggagta tcaccatcga ataactccgg atgctcagca acagagagag cgctgatttg
     1561 ttaacctgat tccggtgaaa aacgctatct tcacaacctt acaaatttcg tggaatgcag
     1621 ctcgcaatct gcgcgttgcg attaatttct acccgatgct tgcgtcaggc tcagatttgc
     1681 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1741 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1801 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1861 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1921 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1981 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     2041 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     2101 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     2161 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     2221 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     2281 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     2341 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     2401 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     2461 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgacgtt
     2521 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     2581 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     2641 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     2701 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2761 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2821 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2881 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2941 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     3001 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     3061 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     3121 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     3181 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     3241 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     3301 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     3361 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     3421 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     3481 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     3541 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     3601 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     3661 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3721 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3781 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3841 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3901 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3961 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     4021 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     4081 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     4141 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     4201 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     4261 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     4321 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     4381 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtactg
     4441 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     4501 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     4561 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     4621 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     4681 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4741 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtaaccgac
     4801 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4861 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact attgctaaac
     4921 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4981 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtacagg
     5041 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     5101 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     5161 cgcgtgcaaa aggtcgtgca gatcgtatcc tgaagcgcac cagccacatc actgtggttg
     5221 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     5281 tcgcctgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     5341 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     5401 gtccgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     5461 tgctcgcccg ggtatcgtta tcggtaagaa aggtgaagac gtagaaaaac tgcgtaaggt
     5521 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     5581 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     5641 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     5701 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5761 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5821 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5881 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5941 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     6001 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     6061 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     6121 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     6181 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     6241 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     6301 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     6361 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcattga agagctgaac
     6421 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     6481 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     6541 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     6601 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     6661 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6721 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6781 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6841 atgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6901 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6961 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     7021 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     7081 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     7141 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     7201 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     7261 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     7321 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     7381 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     7441 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     7501 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     7561 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     7621 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     7681 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7741 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7801 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7861 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7921 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7981 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtatcc gtgacttccg
     8041 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     8101 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     8161 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     8221 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     8281 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     8341 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     8401 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgtccg
     8461 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     8521 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     8581 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     8641 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     8701 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8761 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8821 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8881 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8941 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     9001 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     9061 gttattacga tcaaaggtaa aaacggcgag ctgacccgta ctctcaacga tgctgttgaa
     9121 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     9181 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     9241 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     9301 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     9361 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca ggtgatcggc
     9421 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg caagggtgtt
     9481 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     9541 gataagaaat ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     9601 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     9661 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9721 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9781 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9841 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9901 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9961 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
    10021 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
    10081 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
    10141 gcactctaca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
    10201 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
    10261 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
    10321 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
    10381 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
    10441 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ttgggtctgc
    10501 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
    10561 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
    10621 ccgaaggctc caaaaaggcg ggtaaacgcc tggggcgtgg tatcggttct ggcctcggta
    10681 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10741 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10801 gtaaagcagc gattacagcc gaagttcgtc tatctgacct ggctaaagta gaaggcggtg
    10861 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10921 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10981 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    11041 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    11101 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    11161 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    11221 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    11281 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    11341 ttggcagaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgt
    11401 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctaccgg tctgccgaat
    11461 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    11521 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat cactgaacga
    11581 ggtatcggca acggtatttc aatcattatc ttcgccggta ttgtcgcggg actcccgcca
    11641 gccattgccc atactatcga gcaagcgcgt caaggtgacc tgcacttcct cgtgttgctg
    11701 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tgtttgttga gcgtggtcaa
    11761 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta tgctgcacag
    11821 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11881 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11941 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    12001 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    12061 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    12121 acggcgaagt atatcgataa agtaatgacc cgcctgacct tggttggtgc gctgtatatt
    12181 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    12241 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    12301 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    12361 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    12421 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    12481 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    12541 aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat
    12601 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    12661 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12721 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12781 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12841 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12901 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12961 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    13021 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    13081 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    13141 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    13201 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    13261 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    13321 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    13381 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    13441 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    13501 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    13561 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    13621 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    13681 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13741 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13801 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13861 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13921 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga
    13981 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    14041 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    14101 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    14161 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    14221 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    14281 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    14341 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    14401 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    14461 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    14521 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    14581 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    14641 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    14701 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14761 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14821 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14881 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14941 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    15001 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcttgg
    15061 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    15121 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    15181 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    15241 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    15301 aagactgata gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga taacgagatc
    15361 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    15421 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    15481 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaac gtaaaaaaac
    15541 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    15601 t
//
LOCUS       DAJTRQ010000091        15594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000091 DAJTRQ010000000
VERSION     DAJTRQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..643)
                     /locus_tag="OO332_002889"
     CDS             complement(<1..643)
                     /locus_tag="OO332_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5943302.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALT"
     gene            complement(780..1232)
                     /gene="moaE"
                     /locus_tag="OO332_002890"
     CDS             complement(780..1232)
                     /gene="moaE"
                     /locus_tag="OO332_002890"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5943303.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1234..1479)
                     /gene="moaD"
                     /locus_tag="OO332_002891"
     CDS             complement(1234..1479)
                     /gene="moaD"
                     /locus_tag="OO332_002891"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5943304.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1472..1957)
                     /gene="moaC"
                     /locus_tag="OO332_002892"
     CDS             complement(1472..1957)
                     /gene="moaC"
                     /locus_tag="OO332_002892"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5943305.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(1960..2472)
                     /gene="moaB"
                     /locus_tag="OO332_002893"
     CDS             complement(1960..2472)
                     /gene="moaB"
                     /locus_tag="OO332_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5943306.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2494..3483)
                     /gene="moaA"
                     /locus_tag="OO332_002894"
     CDS             complement(2494..3483)
                     /gene="moaA"
                     /locus_tag="OO332_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5943307.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3471..3615)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3880..4788
                     /gene="yvcK"
                     /locus_tag="OO332_002895"
     CDS             3880..4788
                     /gene="yvcK"
                     /locus_tag="OO332_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5943308.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(4980..7001)
                     /gene="uvrB"
                     /locus_tag="OO332_002896"
     CDS             complement(4980..7001)
                     /gene="uvrB"
                     /locus_tag="OO332_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5943309.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7580..8257)
                     /gene="bioD"
                     /locus_tag="OO332_002897"
     CDS             complement(7580..8257)
                     /gene="bioD"
                     /locus_tag="OO332_002897"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5943310.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8250..9005)
                     /gene="bioC"
                     /locus_tag="OO332_002898"
     CDS             complement(8250..9005)
                     /gene="bioC"
                     /locus_tag="OO332_002898"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5943311.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(8992..10146)
                     /gene="bioF"
                     /locus_tag="OO332_002899"
     CDS             complement(8992..10146)
                     /gene="bioF"
                     /locus_tag="OO332_002899"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5943312.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10143..11183)
                     /gene="bioB"
                     /locus_tag="OO332_002900"
     CDS             complement(10143..11183)
                     /gene="bioB"
                     /locus_tag="OO332_002900"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5943313.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11270..12559
                     /gene="bioA"
                     /locus_tag="OO332_002901"
     CDS             11270..12559
                     /gene="bioA"
                     /locus_tag="OO332_002901"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5943314.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12537..13007
                     /locus_tag="OO332_002902"
     CDS             12537..13007
                     /locus_tag="OO332_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5943315.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13561..14562
                     /locus_tag="OO332_002903"
     CDS             13561..14562
                     /locus_tag="OO332_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5943316.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14596..>15594)
                     /locus_tag="OO332_002904"
     CDS             complement(14596..>15594)
                     /locus_tag="OO332_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5943317.1"
                     /translation="VNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSC
                     HFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLH
                     MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVS
                     GVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFN
                     KPLRYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSG
                     SAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3669 a         4142 c         3911 g         3872 t
ORIGIN      
        1 aggttaacgc gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct
       61 gctcacccat atttttcaat ttctgagtgt catacgccgt caatccgaca aagacaatca
      121 cgccgatgta ggtaactgcc cacatcaatg cttcgctttt cagccagaag ttgaccagcg
      181 atgccagcac aatgccgatt aacgccataa acagcatatt gcccaagcca cttaaatcgc
      241 gcttcgtggt gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag
      301 tactggcgat agaagcagcg gtatagacaa tgaatatact ggaaagcgta agacccgtca
      361 gcgccgaata aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg
      421 ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt
      481 tagtgaacaa cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg
      541 tcagcaacaa gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag
      601 cccggggttg tacgattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat
      661 ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac
      721 gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaaac
      781 taccagcgtt ttgccgcctg cagatcgctc tcccgggctt caacccagcg gtcgccttcc
      841 ggcgtggctt cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc
      901 ccggcttcaa acgcactgct gcgatgcgca ctggtgacac cgacaaaaac gatttcatcg
      961 cccggccata attccccgat gcggtgaatc accgtgacgc gccccagcgg ccagcggtta
     1021 cgcgcttcat caacaatttc tgccagtgct ttttcagtca tccccggata gtgttcgagg
     1081 gttaatgctt tgacgctgtc gcccaggtta tggttgcgca cctttccggt gaaggtgact
     1141 accgcaccgt cttcgtcacg ctccgccagc cacgggtact cttctcctac gctgaacggc
     1201 tgcgggccga caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaggaa
     1261 agctacttcg tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc
     1321 agccagcaat ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccaggtg
     1381 ctggcgtaac gcttcaacgg ttgggtaatc cgcagccact tcggttgcat ctgttcccac
     1441 caactcgcgc acctgggcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac
     1501 ccgacttgcc gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca
     1561 ccgctttgca catgtcataa atggtcagcg ccgccacgga ggccgcggtt aacgcttcca
     1621 tttcgacacc ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct
     1681 ccggctcggc ctgtaaattg acttcaactt tgctgagcat cagcgggtga cacagcggga
     1741 tcagatccca agtgcgtttt gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt
     1801 cgcctttgtg gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa
     1861 aggcttccgc ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg
     1921 cttcgccagc ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg
     1981 gaaattacac ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt
     2041 acggcacgct ttggtcgaac ccggcatggc gaaaatcagc gttttgttag cgacgcccgc
     2101 taccgcacga gattgcaacg tggaagtgcc aatctcttca aacgacaaca tacggaacac
     2161 ttcaccaaaa ccttcaacct cacggtcgaa caacggcagc agcgcttcgg gagcctgatc
     2221 accttccgtc aggccagtac cacccgtaat caataccact tgtacatcgt cgctggcgat
     2281 ccacgcagat acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac
     2341 aacgtgatgg cccgcttctt gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc
     2401 ttcaccgcga cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct
     2461 tacctgactc atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg
     2521 cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc
     2581 tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt
     2641 aacgccgcct tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg
     2701 gttgcaagtg gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta
     2761 atctggatgg caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca
     2821 gccgcgacgc agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag
     2881 ctcgctgccc tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg
     2941 gatccagttc agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt
     3001 gactttgacc ttctcaaaac cggcctcaaa tgcagcatca atccctgcca tgacctggtt
     3061 gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg tccaaactgt cgacactgac
     3121 gttaatgcca gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca gacggtaacc
     3181 attggtggtg accgcaatct ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc
     3241 ggtaaagtcg cggcgtaaag acggctctcc tcctgtcagg cgcacttttt cggtgcccag
     3301 gctggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc ctttattggt
     3361 gacgccgctc ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc
     3421 ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc
     3481 catgtacacc tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat
     3541 ctttacgagc attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga
     3601 gtgtttagtg gttaagccga tactagcgtg taaatgagat ttttaccatc cacactttcg
     3661 ctatgcaatc atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat
     3721 ggcggtttta ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagagat
     3781 agaagaaaat agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg
     3841 cgctactgta gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct
     3901 gatcttgatc gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca
     3961 ctttcgtctt tgggttctcg tttaacgggt atcgtcacca ccacggataa tggtggctcg
     4021 acggggcgta ttcgccgttc agaaggcggc attgcttggg gcgatatgcg caactgcctc
     4081 aaccagctga taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg ttttggcggc
     4141 aatggcgaac tttccggtca taatctcgga aacttgatgt taaaggcgct ggatcacctt
     4201 agcgtgcggc ctctggaagc catcaattta attcgtaatc tgctgaaagt ggatacgcat
     4261 ttgattccaa tgtcagagca tcctgttgat ctgatggcga ttgacgatca ggggcatgaa
     4321 gtttacggcg aggtcaatat cgaccagtta actacgccga ttcaagagtt attgttaacg
     4381 cctaatgtac ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga tctcatcatt
     4441 attgggcctg gcagttttta taccagcctg atgccaattc tgctgctgaa ggaaatcgcc
     4501 caggcattac gccgcacgcc agcgccgatg gtttatatcg gcaatctggg gcgtgagttg
     4561 agtttacctg cggctaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt
     4621 ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa
     4681 gagcggattg tgatccagga ggtactggag gccagcgata ttccgtatcg tcatgaccgc
     4741 cagttgttac ataacgcgct ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa
     4801 ggaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg
     4861 aacgccttat ccggcctaca aaccgcgcaa attcaatata ttgcggagaa aatgtaggcc
     4921 tgataagcgt agcgcatcag gctgttttcc gtttgtcatc agtcttcttc gctatcctgt
     4981 tacgatgcgg caataaacaa ctcacgcaac tgatgcaact ggtcacgaat ttgtgccgct
     5041 tcttcgaact ccagattctg cgcgtgttgc atcatcagcc cttccagttc atggattttc
     5101 tgctgcaacg ctttcggcga catatccatc ggcacattat ccggctcaac aatcgggcgc
     5161 gattttcctc tgcccttcgc tttggttttg gcaatgttct gccccagcgc caggatatcg
     5221 accactttct tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt
     5281 ttctcacggc gacgttcggt ttcgccaatc gctttcgcca ttgatggagt gatcttatcg
     5341 ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac gaccaatggt ctggatcaac
     5401 gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga ggatcgccac cagcgacact
     5461 tccggcatat ccagaccttc acgcagtaag ttgatcccta ccaatacgtc gaactcaccc
     5521 agacgcaagt cgcggataat ctccatacgc tcgacggtgt cgatatctga gtgaagataa
     5581 cgcacgcgct caccgtgttc ttcgagatat tcggtaagat cttccgccat tcgcttggtc
     5641 agtgtggtga ccagcacgcg ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga
     5701 tcatcaacct gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa tccggttgga
     5761 cgcaccacct gatccaccac atcgccgccg gatttttcca gctcgtaatt acccggcgtc
     5821 gccgaaacat agatggtttg cggcgctaat gcttcgaact cttcaaactt aagcggacgg
     5881 ttatccagcg ctgatggcag gcggaagccg tactccacca gtgtctcttt acgcgcccgg
     5941 tcaccgcgat acatgccgcc aatttgtgga atggtgacgt gagattcatc gacgaccagc
     6001 agcccatcgg caggcaggta atcaaacagc gtcggcggtg gctcacccgg tccacgaccg
     6061 gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt catcatctcc
     6121 agatcaaact gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt gttttccaac
     6181 agcactttgc gtctggcggc cagttcttct ttgatctcct ccatcgcctg tacgatgcgc
     6241 tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa aacgtggaat agtggaaaca
     6301 atctgcccgg tcagcgggtc aaataacgac aatcgttcca cttcctcgtc aaacagttcc
     6361 acgcgaagtg caatgtcatc cgattctgcc gggaagatat ctatcacctc gccacgaacg
     6421 cggaaagtac cacgctggaa tgcttgatca ttacgagcgt attgcagctc cgccagtcgg
     6481 cgcagaatcg cgcgctgatc gataatcata ccgaccgtga gatggagcat catcttgaga
     6541 tataaatcag gatcgcccag accataaatc gcggaaacag acgccactac aatcacatca
     6601 cgccgctcca gcatcgcttt ggtggcggac aaacgcatct gctcaatatg ttcgttaacc
     6661 gaggcatctt tctcaatgaa ggtgtcggaa ctcggtacat aggcttccgg ctgatagtag
     6721 tcgtagtagg agacgaaata ctccaccgcg ttttccggga agaactcttt catttcgcca
     6781 tacagctggg ccgccagcgt tttgttgggc gcaagtacca tggttgggcg ctgaaggtca
     6841 gcaatgacat tggcaatggt gaaggttttc ccggagccag tcacgccaag taacgtctgg
     6901 tgcgccaggc catcttccag cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca
     6961 gaaggtttaa aagcggaatt cagtttgaac ggtttactca tgagtcgcta cctgaaggag
     7021 ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat tatgccaaca aattatactg
     7081 gataaaaaaa cagttcatca ccataatatt tctgatacag cgtaaactcc gctgtcaatc
     7141 atgagcaaaa tttactctgt ggcgagataa aactccggcc ttaccgggtt atccccaaag
     7201 caacggcttt tttaacattt gtcaagatga gtgatgacag ttttatggca agagatgcct
     7261 gtacagtgac tgccattgca tttatctaac cagttaaaaa ataaaagata tttctttgag
     7321 ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc tcgcttgcct cgtgttttct
     7381 aactctaata cacatggtta tccacaggaa tagtggataa ctgtctccag cccctatccc
     7441 ccgccgcctg ggcaattccc acattcgccg catgaggcgg aagtaaaatt tttttcgtga
     7501 ttattttaga atttaattgg ttaaattgca gtcaatcgaa gacgcaatct cgctcgcaat
     7561 ttaaccaaat acagaatggc tacaacaagg caaggtttat gtactttccg gttgccgcat
     7621 tttctggatt ttctgcaagc caggggatct ctcccagcag cggcgcggga atcatgcggg
     7681 tgagcgtggt catatattca gcgtgacgtt ttcccggagg cgtaacatcg ttcgccaccc
     7741 aacccgccag agtcagtccg gcgtgttgta ttacctgcgc agtcaacatc gcgtgattaa
     7801 tacagccgag tttcacacca actaccagta tcaccggcag ttgttcctgt gttacccaat
     7861 ctgcaaaagt gaaagtgtca gaaagcggcg taaaccagcc gccagcacct tccactaaca
     7921 cccagtcagc ctgttgttca agcgcgcgta atccggcgct cattaccaat gattctatcg
     7981 gcctgccctc ttgggcgctg atgatgtgcg gcgaggtggg ttctgcgaag gtgtaaggat
     8041 ttaccgttgc gtaatccagc tgcaggctgc tgttgtgctg taacgccagc gcgtcactat
     8101 tacgtaagcc ttccggggtc ttttcgctgc cagaggcgac cggtttataa cctgccgtcc
     8161 gataacctac tgccttcgcg gcttgtaaaa gtgcacaact ggcgacggtt ttccccactt
     8221 cggtatccgt tccggtgaca aaataacgtt tactcacgag caatcactcc caaaaaaaga
     8281 tgatacgtca gaggatatcg cccctgttgt tgcggccagg ccagttgcaa tcgctgcaac
     8341 tgcgaacgcg ttaatattcg cgggtcgcgc ccttcatgaa gatgcgtagc accgatgcct
     8401 ttcagcgaac gcatggcact gagcgcatca tcaaaccata gtgtgatgag ctgaatatga
     8461 tgttgataac gcacgccgtt cagcgactct tcgatttcat ctggcggtaa aaagcgatta
     8521 gcatgcggac gctcgtccac cgcctgccgc gcctgatgca gttcgggtaa cgatccctgc
     8581 accagcgtgg taaacgcgac cacgcctccg gggcgcacca cccgatacag ctcgcggagt
     8641 gccgtggata aattaccgca ccactgcacc gcgagattgc tccatgcaag atcgaacgtc
     8701 gcagtcgcta acggcaggga ttcgatatct cccgccagat aatgatctgc ggcatcttta
     8761 tggcgggctt gcgcaagcat tggcggcgag agatctaacg ccgtcacctg cgcgtgacgt
     8821 tcccgccagt ggcggctcat ccagccaggt ccacaacccg cgtccagtac gtgggtgtat
     8881 ttacgctgtg gaagcattgc cagtaaggcg tcagcactct ggcgctgtag atctgcatgt
     8941 tgctcatagt gtgcggctgc ccgaccaaat gccgctgcaa tggcttgttt attaaccgtt
     9001 gccatgcagc acctccagca gacggtcgat atcctgcatt tcatgcgcag cggttagcgt
     9061 taagcgaagt cgcgcagtac cagcgggtac ggttggcggg cgaatcgccg tgacccagca
     9121 gccttgctgg cgcagttttt ctgccagttg taacgcacgg ctgttatcac cgacgatcaa
     9181 tggctggatg gcgctgcatg aatcagccag cgtaaacggc aaatcctgta ctccggcacg
     9241 aaaacgcgca atgagtgaca ccagtttttc gcgccgtgca tccccctcat cacggcgaat
     9301 gaccgccagc gacgcacgta atgcctgcgc ctgagcgggc ggcatactgg tgctgtagat
     9361 aaggtggcgg gcgaattgca gcagataatc cgccaccgta cttgagcaaa gcaccgcagc
     9421 cccactgacg ccaaatcctt tgccaaaagt cactaccagc aattctggtt ttaccttttg
     9481 cagccagcag ctgccgcgcc cctgctcccc gataacgccc gtgccgtggg catcatcgac
     9541 catcaaccag ccattgtgct gttgcgttac ctgctggatt tccgccagtg gcgcgctatc
     9601 gccgtccatg ctgaacacgc cttctgtcac caccagttgc tgccccggac agggggaagc
     9661 aagcagtcgc gccagatgag cgacatcgtt atgagtaaaa cggcgaagct gcgacgggct
     9721 taaactggca gcctccagca atgaggcatg gctaagccgg tcggcaacaa tacggtcctc
     9781 tttcgccatc atcgcggcaa tgactgcctg attcgcggca aaaccagaga taaacagcag
     9841 cgcccgcgaa tagccaagcc actcggccag ttcttcttcc agcgcctgat gcaccacgct
     9901 ataaccgctg acgtgaccgg agccgccgct accgatgcca aattgctccg ccccctgctt
     9961 ccaggcacgg ataatttgcg gatgatggct taaacctaaa taatcgttac tggaaaagtt
    10021 cagatactgg cgatcatccg ccaccagcca gcgtccggct ccttgcgcca ccggataacg
    10081 gcgacgcagg gcatcggcag cacgccgcgc atcgagcgcc gcgttgattt tctcctgcca
    10141 gctcataatg ctgccgcgtt gtaatattcg tcggtgtccg gggtcatcag cgcctgtcca
    10201 agacgttgct gttgttcgtt atcccctgcc agcacggcag tttgctgcgg atttagcccc
    10261 agtttgcgga acagttgcag gtctttatct tcttccggat tcggcgtggt cagcagtttg
    10321 caaccgtaga aaatcgagtt tgcgcctgcc ataaagcaca tcgcctgagt ctgttcgttc
    10381 atctgctcgc gtccggcaga aaggcgcacg taagaggtcg gcatcatgat ccgcgctacg
    10441 gcaatggtgc gaataaaatc aaaggcatca acgtcatcgt tatcggcaag cggcgtgcct
    10501 ttcaccttca ccagcatgtt gattggtacg ctttccggcg gcgtcggcag gtttgccagt
    10561 tgcagcaata atccggcgcg atcttttacc gtttcgccta agcccacaat gccacctgag
    10621 cagactttga tcccggcatc gcgcactttt tccagcgtat cgaggcgttc ctgataagtg
    10681 cgtgtggtga tgatattgcc gtaaaactcc ggcgaggtgt ccaggttgtg gttgtagtaa
    10741 tccagcccgg cgttcgcgag gcgctgcgcc tgagattcac tcaacgtgcc cagcgtcata
    10801 cacgcctcca gccccatcgc ttttaccccc tgcaccattt gttccaggta cggcatatcg
    10861 cgttcgtggg gattcttcca tgccgcgccc atacagaagc gcgtcgatcc tgccgctttc
    10921 gctttgcgcg ccgactccag cacctgttca acttccatca accgctcggc ttccagcccg
    10981 gttttgtagc gcgagctttg cgggcagtat ttgcaatctt ccggacaagc tccggtctta
    11041 atcgacagca acgtgctgac ctgcacctga cgaggatcga aatgctggcg atgcacctgc
    11101 tgcgcttcaa acagcagatc cagcaacggt ttttcaaata attctgtgac ttgcgacaat
    11161 gtccagcgtg ggcggtgagc catggggctt ctccaaaacg tgttttttgt tgttaattcg
    11221 gtgtaggctt gtaaacctaa atcttttcaa tttggtttac aagtcgatta tgacaacgga
    11281 cgatcttgcc tttgaccaac gccatatctg gcacccatac acatccatga cctcccctct
    11341 gccggtttat ccggtggcga gcgccgaagg ttgcgagcta attttgtctg acggcagacg
    11401 cctggttgac ggtatgtcgt cctggtgggc ggcgatccac ggctacaatc acccgcagct
    11461 taatgcggcg atgaagacgc aaattgatgc catgtcgcat gtgatgtttg gcggtatcac
    11521 ccatgcgccc gccatcgagc tatgccgcaa actggtggca atgacgccgc aaccgctgga
    11581 gtgcgttttt ctcgcggact ccggttccgt agcggtggaa gtggcgatga aaatggcgtt
    11641 gcagtactgg caagccaaag gcgaagcgcg ccagcgtttt ctgaccttcc gcaatggtta
    11701 tcatggcgat acctttggcg cgatgtcggt gtgcgatccg gacaactcaa tgcacagcct
    11761 gtggaaaggc tatctgccag aaaacttgtt tgctcccgcc ccgcaaagcc gcatggatgg
    11821 tgaatgggat gaacgcgata tggttggttt tgcccgcctg atggcggcgc atcgtcatga
    11881 aatcgcagcg gtgatcattg agccgattgt ccagggcgca ggcgggatgc gcatgtacca
    11941 tccggaatgg ttaaaacgaa tccgcaaaat gtgcgatcgc gaaggtatct tgctgattgc
    12001 cgacgagatc gccaccggat ttggtcgtac cggcaaactg tttgcctgtg aatatgcaga
    12061 aatcgcgccg gacattttgt gcctcggtaa agccttaacc ggcggcacaa tgaccctttc
    12121 cgccacactt accacgcgcg aggttgcaga aaccatcagt aacggcgaag ccggctgctt
    12181 tatgcatggg ccaactttta tgggcaatcc gctggcctgc gcggcagcaa acgccagcct
    12241 ggcgattatc gaatccggcg aatggcagca gcaggtggcg gctattgaag tgcagctgcg
    12301 cgagcaactg gcaccagccc gtgatgccga aatggttgcc gatgtgcgcg tactgggggc
    12361 aatcggtgtg gtcgaaacca ctcgtccggt gaatatggcg gcgctgcaaa aattctttgt
    12421 cgaacagggg gtatggatcc ggccttttgg caaactgatt tacctgatgc cgccctatat
    12481 tattctcccg caacagttgc agcgtctgac cgcagcggtt aaccgcgcgg tacaggatga
    12541 aacatttttt tgccaataat ggcgataagt tgccgcatcg tcatgtcttt aacggcatgg
    12601 gttacgatgg cgataatatt tcaccgcatc tggcgtggga tgatgttcct gcgggaacga
    12661 aaagttttgt tgtcacctgc tacgacccgg atgcgccaac cggctccggc tggtggcact
    12721 gggtagttgt taatttaccc gctgataccc gcgtattacc gcaagggttt ggctctggtc
    12781 tggtagcaat gccagacggc gttttgcaga cgcgtaccga ttttggtaaa accggatacg
    12841 atggcgcagc gccgccgaaa ggcgaaaccc atcgctacat ttttaccgtt cacgcgctgg
    12901 atgtagagcg tattgatgtc gatgaaggag gcagcggcgc gatggtcggg tttaacgttc
    12961 atttccactc tctggcaagc gcatcgatta ccgcgatgtt tagttaatca ctctgccaga
    13021 tggcgcaatg ccatctggta tcacttaaag gtattaaaaa caactttttg tctttttacc
    13081 ttcccgtttc gctcaagtta gtataaaaaa gctgaacgag aaacataaaa cgctgtaaat
    13141 atcaatatgt tatgaaagat ttagtctaaa taatagactg cataatacta caaaacacaa
    13201 catatccagt cactatgaat caactactta gatagtatta gtgacctgag gcagagcatt
    13261 agcgcaaggt gattttttgt cctcttgcgc taattttttg tcaacggact gggttagttg
    13321 aatttacaac catgctaagt atctcgataa gcgcagagaa atgatgcagt ggtgggcgga
    13381 ttggcttgat gagaaggtta ggtaggcaaa tccatgcctg agcagagcct tgccctctac
    13441 ctgagcccat gctcattctt tggcctcaac gcgagcatga cctcactggc tggacccaaa
    13501 atggactatg acagaaagat ctgatatttt tcctcgcaaa aacttagagg ctttatttta
    13561 atgcataaga gcaactgctt tgacacggca aggcttgtgg cagctatgat ggttctggtc
    13621 agtcaccact acgcgctctc tgggcagccg gagccgtacc tgttcggctt tgaaagtgct
    13681 ggcggcattg cagttatcat attcttttca atatcaggat atctgatttc aaaatcagct
    13741 atcaggagtg attcctttat tgatttcatg gctaaaagag cgagaagaat attcccggcg
    13801 ctggtccctt gctctatact gacatatttt ttatttggat ggatacttaa cgacttcagc
    13861 gcagaatatt ttagccatga cattgtcagg aagaccataa gctctatttt tatgagtcag
    13921 gctccagatg ctgacattac atcacattta attcatgccg gaattaacgg cagtttgtgg
    13981 accctaccgc ttgagttcct gtgttatata ataacgggag tagcagttgc cctactaaag
    14041 aacggaaaag cattcatagt tatactgttg gtttttgttt ctctttcatt aattggaagt
    14101 gtgtcagaaa acagggatgt aatgttctct attccgttat ggctgtatcc tttgcgtgga
    14161 ctggcgttct tttttggtgc cactatggca atgtatgaga agtcatggaa tgtaagtaat
    14221 gttaaaatta ctgtagtttc tttgcttgct atgtacgcct acgctagtta tggcaaaggc
    14281 attgattata ctatgacttg ctatatcctg gtttcattct cgacaatagc catatgcaca
    14341 tccgttggcg acccactggt taagggcagg tttgattact cgtatggtgt gtacatttat
    14401 gcattcccgg ttcagcaggt tgttataaac acattgcaca tgggttttta cccgtcaatg
    14461 ttgttaagtg ccgtgactgt tcttttcttg tcccatcttt catggaatct ggttgaaaaa
    14521 agattcctga cacgaagttc gcctaaatta tccctggatt gaacaataaa aggggccata
    14581 tggcccctaa aataatcaga tggccagata ctcaaccatt gaaggcagcg cgactcctac
    14641 tgggcttggg cgagtaacat taattgtcac cacggaaaca ctaaccttgt caccacttga
    14701 tgcagggtca ccaaaaaggc ttcttactct gtatgtcagg ttccccgggt tcggtatagc
    14761 tgcagatccg cttgtggcac ctaccgcaac tgggacatta atgatcccat ttacagacgg
    14821 tacgcctgaa cagccacgat acgtattgta tctgccttcc ggtttctgca cctcggtaat
    14881 gccagccaaa taacagtcag taactgacac gccaccgcta cgaagatagc gtagaggctt
    14941 attgaatatg cacttatcga aagaaacaca ttcattgatg tcgacaatat ttacagccca
    15001 ttcatcacca gttaacgtac aaccacttac aaagacgaag atcgccgact gttctatcgc
    15061 tatactttga ttgtttgcag ccgtgttttt gtttgcagtg ttgatatgaa caccactgac
    15121 aattttatgt gactgcgttg aggtcggcgt tctaaaatat atccccgtaa attgttcagt
    15181 gccatcggca tctgctgcta tccatgagtt tgagaaagaa aatcctccgt ttacattctc
    15241 tataaccacg cctgttgaac cgcaataatc taaatccagt tgttccatat gcaaatcaag
    15301 gcaatcatga acatagacgg catttttaaa accaatgcac atggtctcac tgtctaaaat
    15361 tattgcttct gacctaactg cctctgacga ccacgcatac gtttgaggct gaatcctgat
    15421 gccaaagctt tcatcagcag aataatttcc tctgctgaaa tggcaggagc tgacactaac
    15481 acttacgcac tggcctaaaa agtgaagacc gactctacag gttccagcct gacatgctat
    15541 aaattttgag tgccagagcc cttgcattaa ataagcggtt tcaacttcat tcac
//
LOCUS       DAJTRQ010000092        15541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000092 DAJTRQ010000000
VERSION     DAJTRQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..947)
                     /locus_tag="OO332_002905"
                     /pseudo
     CDS             complement(1..947)
                     /locus_tag="OO332_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            complement(1350..1595)
                     /locus_tag="OO332_002906"
     CDS             complement(1350..1595)
                     /locus_tag="OO332_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943318.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(2240..2315)
                     /locus_tag="OO332_002907"
     tRNA            complement(2240..2315)
                     /locus_tag="OO332_002907"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2280..2282),aa:Phe,seq:gaa)
     gene            complement(2421..3128)
                     /locus_tag="OO332_002908"
     CDS             complement(2421..3128)
                     /locus_tag="OO332_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5943319.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3526..5661
                     /gene="speC"
                     /locus_tag="OO332_002909"
     CDS             3526..5661
                     /gene="speC"
                     /locus_tag="OO332_002909"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5943320.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5710..6966)
                     /gene="nupG"
                     /locus_tag="OO332_002910"
     CDS             complement(5710..6966)
                     /gene="nupG"
                     /locus_tag="OO332_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5943321.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7168..8247)
                     /gene="mltC"
                     /locus_tag="OO332_002911"
     CDS             complement(7168..8247)
                     /gene="mltC"
                     /locus_tag="OO332_002911"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5943322.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8312..8587)
                     /locus_tag="OO332_002912"
     CDS             complement(8312..8587)
                     /locus_tag="OO332_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5943323.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8615..9667)
                     /gene="mutY"
                     /locus_tag="OO332_002913"
     CDS             complement(8615..9667)
                     /gene="mutY"
                     /locus_tag="OO332_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5943324.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9828..10547
                     /gene="trmB"
                     /locus_tag="OO332_002914"
     CDS             9828..10547
                     /gene="trmB"
                     /locus_tag="OO332_002914"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5943325.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10547..10873
                     /locus_tag="OO332_002915"
     CDS             10547..10873
                     /locus_tag="OO332_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5943326.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            11057..11776
                     /locus_tag="OO332_002916"
     CDS             11057..11776
                     /locus_tag="OO332_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5943327.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11952..12998
                     /gene="ansB"
                     /locus_tag="OO332_002917"
     CDS             11952..12998
                     /gene="ansB"
                     /locus_tag="OO332_002917"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5943328.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13115..14122
                     /locus_tag="OO332_002918"
     CDS             13115..14122
                     /locus_tag="OO332_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5943329.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(14178..15479)
                     /locus_tag="OO332_002919"
                     /pseudo
     CDS             complement(14178..15479)
                     /locus_tag="OO332_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(15479..>15541)
                     /locus_tag="OO332_002920"
     CDS             complement(15479..>15541)
                     /locus_tag="OO332_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5943330.1"
                     /translation="ALCFINQSIQQLKQPAQEAS"
BASE COUNT         4008 a         3761 c         4009 g         3763 t
ORIGIN      
        1 acttccacgc agattatgcc gtttcagttg cgcctgataa tctgctgaac agtactggcc
       61 tccacggtcc gtgtgaacga taacgttccg gggcctctta cgccgccaca gcgccatctg
      121 cagggcatcg caggccagtt gcgccgtcat gcgtggcgac attgaccagc caataacggc
      181 acgtgaccac aggtcaatga ccactgccag atacagccag ccttcatctg tacgtaagta
      241 cgtgatgtct cctggccact tctggttcgg gccactggcg taaaaatcct gctccaacag
      301 attttctgac acaggcaggc cgtgtgcgcg gtagctgacc gggctgaact tccgggaggc
      361 ctttgccctc agtccctgac ggcgcaggct tgccgccacg gtttttacgt taaaggggta
      421 accctgagca cgcagttcat ccgtcaggcg tggggcaccg taacgctgtt ttgaccgggt
      481 aaaagccgcg aggacaacgc tgtcgcagtg ttggcggaac tgctgacgcg tgcttatcct
      541 tgtccgccgc tgacaccacg tataccagcc gctgcgggcc acccggagca cgcggcacat
      601 tgctttgatg ctgaactcag cctgatgttt ttcaataaag acatacttca tttcaggcgc
      661 ttcgcgaagt atgtcgcggc cttttggagg atagccagct cttcatcccg ttctgccagc
      721 tggcgtttga gacgtgcaat ctcggtagac atctccagtt cacgttcaga agacgtctgc
      781 tgattttgct gtttactgcg ccagttgtag agctgtgatt catacaggct gagttcacgg
      841 gctgcggcag taacaccgat gcgttcagca agcttcaggg cttcactgcg aaattcaggc
      901 gaatgctgtt tacggggttt tttactggtt gatactgttt ttgtcatgtg agtcacctct
      961 gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat caggctgagg
     1021 agtccccaga aaagaagaca ggcatagagt tttgtgatct actgattgcg ctaacaaatt
     1081 caatgagatc acctctatgc ctgcccgtta agtatgtcag gcttttttcc gtaatgctct
     1141 aacttccacg cattggtttc tgacatcaaa taaggctatg actttctctc ccttttaatc
     1201 tcattctgat ctaaaaccat atcttaaaaa aatgcatggg tattttaatt attttgatgt
     1261 tgatctaacg cactcaagga ccattttatt accgattatc cattctattt aactgttgcg
     1321 atataaatgt ttgttttagg tgctttaatt cagtacaagt catcaaaccg taccagaccc
     1381 tgactgtcag tgacaccgac aggtgggggc ggcggtgtgg ggtcaaactg agtgtgtctg
     1441 cctctggccc acagagccag ctctgccgcc attgcccgtg gatgatactc cagctgcacg
     1501 ttgagaccgg aaactgggtt gttgttctca tccacgaact gcagcgccag ccagtgcatc
     1561 cgctgcttac cgcaggcgac ttctttgtac ttcattttgt gactcaggca ggtgactgtc
     1621 gtgttgccgg ttgcgtccat tttgcggtta aaccctttta attcaatttg ttaaattgct
     1681 ttccaccttt tgcggggcgc gacttaacca aaatattcac tgttatcata atatgcaatg
     1741 ggctggaggt tgcaggtcat ttagggattt tctgtaagcc ccgttttgca gaacgttcta
     1801 tctccgattg agatcctgtc tctcggtatt accggtagtc tggccataat cccctatgga
     1861 tcatattgat atagtaggca taacacccaa aagttgacta aagctaaatc aatcagagtg
     1921 aaaaatgttc ggtttgatgt agggggggac tgaggagggg ccaacgcagg tggtatgcgt
     1981 tttacttcag accggacaaa gaccggatgt atccgtgcag taatgaatcc gttagccagg
     2041 tgctcatgtg gaaatagtgt ctattgggga tttgaagttt gacagaaaag gccacagtga
     2101 ccagaagggg gctacttaga agaaatgttc aggctggatg gaagaggtaa agcagtgaca
     2161 tcagcattca aagtgaccgg atatctacca gaaccccaga aagcaaaaaa ccagcccgta
     2221 ggctggttct tctaagtagt ggtgcccgga ctcggaatcg aaccaaggac acggggattt
     2281 tcaatcccct gctctaccga ctgagctatc cgggcaacgg ggcgcattaa acctgattcg
     2341 cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac tgcacaatct atcatcactt
     2401 tgccgctatt gcacgcattg tcaggcaaac caggcggtcc aggcggcgga aagcggcatt
     2461 gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc acagatgcgt
     2521 aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat catcgacggt
     2581 gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat gatcagtaat
     2641 gggatactaa ttaccgacac cgcaataccc agcgagcagg cgaagatcat cgccgtaaag
     2701 aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc ttcgttcatc
     2761 gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac ataattctga
     2821 ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg tgctttggcg
     2881 actgctgtat tgacaccttt ctccagcaaa caaatttcgc caattagagc gccaagtagc
     2941 gtagctaaaa ccatcgctgg aaggttggcg catttcacaa ccagtaaaat accaatcccc
     3001 agcgatgcca gaccaaaaat agatgtcatg gagacgcgga tacgttccgg taagcgttgg
     3061 ctgagcagtg cgccgagaac gccacccagt aagactgcac tagcgttgat aaaagggccg
     3121 atgaccacaa gagttcctgt tagctgtatt gtttgatttt cttattatga actttttgcc
     3181 ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gccttagcga aaggtgccat
     3241 gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca tctccttaca
     3301 ttctctcgct tatcgccgtt tcgcgcgaaa cgtttccctt tctatgttac tgctcatgcg
     3361 gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagcgttt ctgcttttac
     3421 tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg gcagggtttt
     3481 ccaccttgtc cggtattctt acttccccga aacgggtttg cgcttatgaa atcaatgaat
     3541 attgccgcca gtagtgaact ggtatcccga ctttcttctc atcgccgcgt ggtggcgttg
     3601 ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga tagtcgcagt
     3661 ggcattcttg cgttgcttaa gcgcaccggt tttcatctac cggtgttttt gtattccgaa
     3721 catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga gcagcagtgg
     3781 ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc gttttatgac
     3841 acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg acatcaacat
     3901 ggtgcgttct ttaaaaagca tcctgccgga cgccattttt acgatttctt tggtgagaac
     3961 gtctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct tattcatgaa
     4021 ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga taaaacctat
     4081 tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct gttaacgcgt
     4141 ggcgatctgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg cgcgctgatt
     4201 caggcgggga cgacgccggt ctatctggaa gcttcacgta acccgtttgg tttcattggc
     4261 ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg cgacgttgcg
     4321 ccagaaaaag ccgacctgcc gcgcccgttt cgcctggtga ttattcagct gggaacctat
     4381 gacggcactg tctataacgc ccgtcaggtg atcgatacca ttgggcatct ctgtgattac
     4441 attctgtttg attccgcgtg ggtcggttac gaacagttta tcccgatgat ggcggatagc
     4501 tcgccgctgc tgttagaact taacgaaaac gatccgggga tttttgtgac ccagttggtg
     4561 cacaaacagc aggctggatt ctcacagacg tcgcagatcc ataaaaaaga taaccatatc
     4621 cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat gctccatgct
     4681 tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa aattcatgaa
     4741 ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga gtcgcgcaag
     4801 gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt tgatggcaaa
     4861 ttgtggcagg attatccgac atcagtgtta gccagcgacc gccgtttttt cagttttgag
     4921 ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt tgttgatccg
     4981 tgcaagctgt tactcactac accaggtatc aatgccgaaa ccggcgaata tagcgacttt
     5041 ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt gccggagaag
     5101 tgcgatctca actccattct gttcttatta actccggcgg aaagccacga gaagctggca
     5161 caactggtgg tgatgctggc gcaatttgaa cagcatattg aggatgactc gtcgctggct
     5221 gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta caccctgcgc
     5281 cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga cctacaaaaa
     5341 gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga tgcgcatagc
     5401 gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg gcgaattgcg
     5461 gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg ggaagtctgg
     5521 ggtggggcgg ttcaacgtta tttccttgcg ctggaagaag gcgtgaattt gttgccggga
     5581 ttttcgccgg agttgcaagg tgtctatagc gaaaccgatg cggatggcat gaaacgattg
     5641 tacggttatg tgttgaagta agaataaaaa aacgggtcac cttctggcga cccgtttttc
     5701 tttgcgtaat tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt gtttatattt
     5761 gaacatcgcc atgaacgcga aggccagaac cacggaataa ccagcgaaaa tcaaccatac
     5821 ggtctgccag tcggtaatgc cgttttgggt gtacatctca acaactttac cgctcacgat
     5881 gccgccgagg atacagccga agccgttagt catcatcagg aacgtccctt gtgcactggc
     5941 gcgaattgcc gggctaactt ctttttcgac aaacaccgaa ccagagatgt tgaagaagtc
     6001 gaatgcgcaa ccgtaaacga tcatcgacag taccagcagt acagtaccga acggagtcgg
     6061 gtcgccgtaa gcaaacagcg caaaacgcag gatccacgcc acaatactga tcatcattac
     6121 gttcttaata ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca gggtttcaga
     6181 gatctgcgaa atcgacatga tgattgacgc atgctgcacg atgaagctgc tggcaaacat
     6241 cggatctttg tcgaagctgt gcaggaaggt attaccgaac atgttggtaa tctgcagttc
     6301 cgcgcccagc agcattgaga agatgaagaa gattgccata cgcttgtttt taaacagcgc
     6361 gaatgcatcg aggcccagca gggttgtcca gctctgattc gcttgctgtt tagcaaccgg
     6421 aatatgcggc agagtcaggg taaacagaac cagaatggcg gaaagtgctg cgccaatata
     6481 cagctgcatg tggcttaatt cgaagccaga caggctcacc acccacattg ccatgataaa
     6541 gccgatggtg ccccagatac ggattggcgg gaagtcagta acgatatcca tcccggcatt
     6601 ttgcaggcga tagtaagaga tggtgttgat taacccaagc gttggcatat aagcaaacga
     6661 gttaatcaat atcacaagga acatcgcttc cggcgtcgtg acctgtgccg ccatgaacag
     6721 cgtgatagcg ccaatggtgt ggcaaatggc atatacccat ttcgcactta accatttgtc
     6781 ggccacaatc cccagtagcg caggcataaa taccgcggcg atacccagtg agctataaac
     6841 tgcgccaata gaagcaccgt caaacttcag ggtaacaaac atataggagc cgagggtcgt
     6901 cagccaactt ccccacagac agaactgcag aaaagagagg attttcagct gcagcttaag
     6961 attcatgtta atttcctcac atcgtgattc ggatgagtgt ttgaaaaagc acatttgacc
     7021 gtgcgagatg atgggattct atcaacttcg cggactggtt ttttgttacc tgtggcaaat
     7081 aattgcaaac attcccaatt tgcaaaacga aattgtgatg tggataacat ttttgcccct
     7141 gagcatcgtc aggggcggtt aatggaatta tcggcggcgg taggattttt gcgcggtatt
     7201 cactttataa agataacggc gagattccgc agagggatgg cgggtcgtca gcgtctgata
     7261 aacatcgccc ggcgtcatgg tgttaataat attggcagcc tgaatcttat cattcgaaaa
     7321 gactcgcagc acgctgcctg cgccgccgtt ataggcggtg atgacggcat aacgccgcga
     7381 tgttgggtta tcaattccgc cgagataaac attgttcagc atcgccagat acgcggtgcc
     7441 ggtatcaata ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc cggatttccc
     7501 ctgcgagcgg aacacatctt tcccggcagt attttgtacc acctgcatta atcccagcgc
     7561 atcggaacgg ctgaccgcat acgggttaaa ggaagattca gtctgcataa ttgccagaat
     7621 cagcgactca tcaacgccat atttccgtga cgcctggcgg accatgccga gatatttgtg
     7681 cgcacgttta tcaaggtggt tcggcaccat gttaatggtg acgctgtaga tgatacgtag
     7741 cccgttgctg cggcttttca gacggttttt cagcagataa tccgcgaagt tgcttgcgcg
     7801 accttcccag cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa ggaaaggttc
     7861 tttcgaaatt gtaatatcat caacgtcgga atagagatcg accgaactcg gatcgtcacc
     7921 catcagcaac gttttgataa ttgcccggcg caaatgcgcg gcaggttctg tcccggcgat
     7981 ggtttcgata gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct gatattgatc
     8041 ggtgtatttc acgtagtcct taggaccggc gatcaccacc tctttgaagc cccagatgtt
     8101 ctcaatattg tgggcaaatt gccccatcag aatatcaaaa ccgttggtat ctttgaccca
     8161 ggcttcgtta taggtatcgc cttttttggt cgtcgaacag gagatgagca acggcgcaat
     8221 caaagccagc gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt gtttttatgc
     8281 aagctgccgg aggcgtgctc cggcactgtt tttatttttt atcttccggc gtatagccct
     8341 cgatatgcac ctctttaccc tcgaacagga agttgaccat ctcctgctcc agcagcttgc
     8401 ggtgctcggc attcatcatg ttgagtttct tttcattaat cagcatggtt tgtttgtgct
     8461 gccactgcgc ccaggcttct ttggagatct cgttatagat gcgttttccc agctcgccgg
     8521 ggtacagctg aaaatcctga ccttctgctt cacgttgcag gaaagtacaa aaaatcgttc
     8581 tgctcataaa tcatcctctt tatcgactca cgcgctaaac cggcgcgcca gtgcgtaact
     8641 gctgtaacaa acgctccacg ggagccgcca ggccaactga cggcggttgc gctaagttat
     8701 accagagcgc attgccttca tccatgcagc cggtgaatga cgacacggga agccacatag
     8761 gcacaatatc taagtggaaa tggctgaagg tatggcgaaa cgcggtcagt tgcgtcaggt
     8821 tatcggcagc aatctgccgt tgtgccagcc aatgccgcaa actttcttcg tcggcaaact
     8881 tcgggaaaca gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc agcaatactt
     8941 catcttcgtg ctgtaacagg agaaagtagc cggtgcgctc cggcagcatc tgtttcggtt
     9001 ttttgcccgg ataaagcgac cagctattgt tggtggcggc aatacatccg ttttgtagcg
     9061 gacagagcga acatttcggc ttcgaacgcg tgcaaatcat cgcgcccaaa tccatcatcg
     9121 cctgattaaa ccgttccacg ccgaccgcgg gcgtcacctg ctcgctcaaa ctccatagtt
     9181 tattctcgac ctcttttttc ccaggccagc cgcttacagc atagcagcgc gccagcacgc
     9241 gtttgacgtt accgtcgaga atcggaaagt gcttgcccag agaaagcgag agaatcgcgc
     9301 ctgcggtgga acgcccgacg cccggtaacg gcgcgacttc ttcaaaggtt tccgggaatt
     9361 taccaccgtg taaggtcgcc acttgttgtg ccgctttatg caggttgcgc gcgcgggcgt
     9421 aatagccaag cccggtccac aagtggagaa cttcgtcgag cggcgcattg gcgagatcgg
     9481 tcaccgtcgg gaagtgcgcc ataaagcgtt caaaataggg gataacggtc gcaacctgag
     9541 tttgttgcaa catcacttct gagagccata ctttgtaggg cgtcttgtca atttgccagg
     9601 gcagcgtttt ccgcccgtat ttatcgtacc agtccagaac ctgggctgaa aattgcgacg
     9661 cttgcatggt caccgacttc actgttgttg ggggcaagat tgcagcacag cgacggcagg
     9721 gtgtaaaccg gaactttccg cagccacggc cttatcatgc ttgcatccgg caactaactt
     9781 tggataatgc ccgttttcag aacactttca caagcgacta aatctttatg aaaaacgacg
     9841 tcatttcacc ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt agttttgtgc
     9901 gccgccaggg gcgactgacc aaaggccagg aacatgcgct ggaaaattac tggccggtga
     9961 tgggcgttga gttcagtgaa gatatgctgg atttccccgc gctttttggc cgtgaagcgc
    10021 cggtgacgct tgagattggt tttggcatgg gggcgtcgct ggtggcaatg gctaaagatc
    10081 gccccgagca ggacttcctc ggcattgaag tgcattcacc gggcgttggt gcgtgcctgg
    10141 cttctgcgca tgaagaaggt ttaagcaacc tgcgcgtgat gtgtcacgat gcggttgaag
    10201 tgctgcataa aatgattcct gacaattcat tgcgcatggt gcagctcttt ttccctgacc
    10261 cgtggcacaa agcgcgccat aataaacgcc gtatcgttca ggtgccgttt gccgaactgg
    10321 taaaaagcaa actgcagctg gggggcgtat tccatatggc gaccgactgg gaaccttatg
    10381 cggaacatat gcttgaagtg atgtcttcta ttgacggtta taaaaacctg tcagagagca
    10441 atgattacgt accgcgtccg gcatcacgtc cggtgacgaa atttgaacaa cgtggtcatc
    10501 gtcttggtca cggagtatgg gacttaatgt tcgagagggt gaaataatgg caaagaaccg
    10561 tagccgtcgt ctgcgtaaaa aaatgcacat cgacgaattc caggaattag gattttcggt
    10621 ggcatggcga ttcccggaag gtacatcgga agaacagatt gataaaatcg ttgatgactt
    10681 tattaacgag gttatcgaac cgaacaaact ggcctttgac ggcagcggtt atctggcctg
    10741 ggaaggtctg atctgcatgc aggaaatcgg caaatgcacc gaagaacatc aggcgattgt
    10801 gcgtaagtgg ctggaagagt gcaaactgga tgaggtacgc accagcgaac ttttcgacgt
    10861 ttggtgggac taagaaagca tacgggcgat gacaaatgca aaactgcctg atgcgctacg
    10921 cttatcaggc ctacagagat gcacgatcga gtaggcggga taaggcgttt acgccgcatc
    10981 cggcatggaa aacgcgtact ttgttatcaa tctggggcca gcatatgctg gcctgatttg
    11041 ttcttgaggg aagactatga tgcgcaaaat gctgctggcg gcagcacttt cagtgacggc
    11101 aatgaccgct cacgccgact accagtgcag cgtcacgccg cgtgacgatg tgattgtcag
    11161 cccgcaaacc gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc cagacggcaa
    11221 cgtgatgtat aacggtaagc aatattccct gaatgccgcc cagcgcgagc aggcgaagga
    11281 ttatcaggct gaactacgta gcacgctgcc gtggattgat gaaggcgcga aaagccgcgt
    11341 cgagaaagcc cgtattgcgc tggataaaat tatcgttcag gagatgggcg acagcagcaa
    11401 aatgcgcagc cgtctgacca aacttgatgc gcagcttaaa gagcagatga atcgcattat
    11461 cgaaacgcgc agcgatggcc tgacgttcca ctataaagcc attgatcagg ttcgcgccga
    11521 aggccagcaa ctagtgaatc aggcaatggg cggaatttta caggacagca ttaatgaaat
    11581 gggcgcgaaa gcggtgctga aaagcggcgg taacccatta cagaatgtgc tgggaagctt
    11641 gggcggtctg caatcctcaa tccaaaccga gtggaaaaag caggaaaaag atttccagca
    11701 gtttggcaaa gatgtttgta gccgcgttgt gactctggaa gatagccgca aagccctggt
    11761 cgggaattta aaataatcct ctattttaag acggcataat acttttttat gccgtttaat
    11821 tcttcgtttt gttacctgcc tctaactttg tagatctcca aaatatattc acgttgtaaa
    11881 ttgtttaacg tcaaatttcc catacaaagc taagggataa tgcgtagcgt tcacgtaact
    11941 ggaggaatga aatggagttt ttcaaaaaga cggcacttgc cgcactggtt atgggtttta
    12001 gtggtgcagc attggcatta cccaatatca ccattttagc aaccggcggg accattgccg
    12061 gtggtggtga ctccgcaacc aaatctaact acacagcggg taaagttggc gtagaaaatc
    12121 tggttaatgc ggtgccgcaa ctaaaagaca ttgcgaacgt taaaggcgag caggtagtga
    12181 atatcggctc ccaggacatg aacgataatg tctggctgac actggcgaaa aaaattaacg
    12241 ccgactgcga taaaaccgac ggcttcgtca ttacccacgg taccgacacg atggaagaaa
    12301 ctgcttactt cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg gtcggcgcaa
    12361 tgcgcccgtc cacgtccatg agcgcagacg gtccattcaa cctgtataac gcggtagtga
    12421 ccgcagctga taaagcctcc gctaatcgtg gcgtgctggt ggtgatgaac gacaccgtac
    12481 tggacggtcg cgatgtcacc aaaaccaaca ccaccgacgt agcgaccttc aagtctgtta
    12541 actacggtcc tctgggatac attcacaacg gtaagattga ctaccaacgt accccggcac
    12601 gtaagcacac cagcgatacg ccattcgatg tctctaagct gaatgagctg ccgaaagtcg
    12661 gcatcgttta taactacgct aacgcatccg atcttccggc taaagcactg gtagatgcgg
    12721 gctatgatgg catcgttagc gctggtgtgg gtaatggtaa cctgtataaa tccgtgttcg
    12781 acaccctggc aaccgccgcg aaaaacggca ctgcagtagt gcgttcttcc cgcgtaccga
    12841 cgggtgctac cactcaggat gctgaagtgg atgatgcgaa atacggcttc gtcgcctctg
    12901 gcacgctgaa cccgcaaaaa gcgcgcgtcc tgctgcagct ggctctgacg caaaccaaag
    12961 atccgcagca gatccagcag attttcaatc agtactaatc acctcgtccc ggcatcgtgc
    13021 cggggctttt tcacttcaga cttacgtcca ttgccaattt tcattaccct aatgataatc
    13081 accggaataa attattccgc gcgagggttt tcgggtgaaa aagcaatgga ttgttggcac
    13141 ggcgctgctt atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc caactgaaaa
    13201 tatcttaaaa gatcaattca aaaagcagta tcacggcatt ctcaagcttg atgccatcag
    13261 cttaaaaaat cttgatgcta agggtaatca ggccacctgg tcagcggaag gcgatgtctc
    13321 ttccagtgac gatctctata cctgggtcgg tcagttggca gattacgaac tgctcgaaca
    13381 gacctggacg aaagataaac cggtaaaatt ctcggcgatg ttaaccagta aaggaacgcc
    13441 cgcgtctggc tggtcggtga acttttactc ttttcaggcg gcagccagcg atcgtgggcg
    13501 ggtggtcgac gatatcaaaa cgaataataa atatctgatc gtgaatagcg aagatttcaa
    13561 ttatcgcttt agtcagcttg aatctgcgtt gaatacccag aagaactcga ttcctgcgct
    13621 ggaaaaggaa gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag cagcggatgc
    13681 atattgggga aaagatgcaa acggtaagca aatgacccgc gaagaagcct ttaaaaaaat
    13741 tcaccaacag cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg cggttaaata
    13801 tgacaaagag gtttatcaac cggcgattgc ggcatgtcat aaacagagtg aagagtgtta
    13861 tgaagtgccg attcagcaga aacgagattt cgatatcaac gaacaacggc gacagacttt
    13921 tctgcaatca caaaaactca gccgtaaatt gcaggatgac tgggtaacgc tcgaaaaagg
    13981 gcaatatccg ttaacgatga aagtttcaga aataaacagt aaaaaagtcg cgattttgat
    14041 gaaaattgac gatatcaacc aagctaatga acgctggaaa aaagataccg aacaactgcg
    14101 gcgcaatggg gtgattaagt aactcatgtt aagtgccttt gccggataat ataaaaagtt
    14161 atccggcaaa ttatttttta cattagccct agtagctgtg gaatgaacaa ggatatttgc
    14221 ggaatgtaag tgatcatcat caacgctact agcaatgcag cataaaatgg cagcaatggt
    14281 ttgattaaat gttgaatctt cacacccgag atagaacaac caacaaatag cgcactaccc
    14341 accggcagtg ttaacagccc aatacaaagg ttggccacca tcataatacc gaaatgtact
    14401 gggtccattc ccagctcttg tgcaatcggc aagaagatcg gtgtaaagat tagtacagcc
    14461 ggtgtcatgt ccataaagat gccgacaatt aataagacga tattaatcag cagcaatatg
    14521 atcagtgggt tatcagaaat ccccatcaat gcgtcgctga tcatataagg aatatcagca
    14581 ttagtcattg cccaggacat gcccacggaa aaaccgatca acagtaatac aatcgaagtg
    14641 gtaacaaccg actccagaat cagtttgggt aaatggcgcc attttacttc ccggtaaatc
    14701 agcaccgaga gaataaatgt gtagacaacg gcaattgctg acgcttcagt tgcggtaaaa
    14761 atgccgccca gaatgccgcc catcacaatg aacaccagaa gcaaacttgg taaggcatcc
    14821 agaaacgctt tacaggcctg agcaaaggtt gctctttctg aaagggggta gccacggcgt
    14881 ttagcaataa tgccgcagac tatcattacc gcaagaccca ttaagatccc tggcaaatat
    14941 cccgccataa acagcgatgc tacagaaacg ccgccagctg tcagggaaaa cacaattaac
    15001 acgtttgagg gcggtatcag taatccagta atacaagatg agacgtttac agcggtagaa
    15061 aatgccggat cataaccttc tttggtctga atgggattca gtgtgccacc aacggcagca
    15121 gctgccgcaa cggctgaacc agaaatggaa ccaaacatca tattcgccag gacgttcaca
    15181 tgaccaagcg aaccaggaac gcggccaacc attacctagg cgaggttaat caaacgtatc
    15241 gcaataccgc cgctgttcat cagtgttccg gcaaagatga aaaaggggat tgccaacagg
    15301 gcaaagttat ccagtccatt agccagtcgt tgcgaaacag tgattgccgc gatatcccag
    15361 ggaaacatca aaagcattga agctacagtg gcaataccga ttgagaacga aatgggcaca
    15421 ccaataaaga ccagcacaaa aaaacagcca aacagtgtaa tcgcgatata actatccatt
    15481 aactggcctc ctgcgcaggt tgtttcagtt gttgaatgct ttgattaata aaacacaagg
    15541 c
//
LOCUS       DAJTRQ010000093        15400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000093 DAJTRQ010000000
VERSION     DAJTRQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..1165)
                     /locus_tag="OO332_002921"
     CDS             complement(<1..1165)
                     /locus_tag="OO332_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5943331.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGE"
     gene            complement(1364..1448)
                     /locus_tag="OO332_002922"
     tRNA            complement(1364..1448)
                     /locus_tag="OO332_002922"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1412..1414),aa:Leu,seq:caa)
     gene            1644..2663
                     /gene="ahr"
                     /locus_tag="OO332_002923"
     CDS             1644..2663
                     /gene="ahr"
                     /locus_tag="OO332_002923"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5943332.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2792..4294
                     /locus_tag="OO332_002924"
     CDS             2792..4294
                     /locus_tag="OO332_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5943333.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4413..5495)
                     /gene="lptG"
                     /locus_tag="OO332_002925"
     CDS             complement(4413..5495)
                     /gene="lptG"
                     /locus_tag="OO332_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5943334.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5495..6595)
                     /gene="lptF"
                     /locus_tag="OO332_002926"
     CDS             complement(5495..6595)
                     /gene="lptF"
                     /locus_tag="OO332_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5943335.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6862..8373
                     /gene="pepA"
                     /locus_tag="OO332_002927"
     CDS             6862..8373
                     /gene="pepA"
                     /locus_tag="OO332_002927"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5943336.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRTGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8507..8950
                     /gene="holC"
                     /locus_tag="OO332_002928"
     CDS             8507..8950
                     /gene="holC"
                     /locus_tag="OO332_002928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5943337.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8950..11805
                     /gene="valS"
                     /locus_tag="OO332_002929"
     CDS             8950..11805
                     /gene="valS"
                     /locus_tag="OO332_002929"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5943338.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11859..13055)
                     /locus_tag="OO332_002930"
     CDS             complement(11859..13055)
                     /locus_tag="OO332_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5943339.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13248..13751
                     /locus_tag="OO332_002931"
     CDS             13248..13751
                     /locus_tag="OO332_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5943340.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13797..14213)
                     /gene="rraB"
                     /locus_tag="OO332_002932"
     CDS             complement(13797..14213)
                     /gene="rraB"
                     /locus_tag="OO332_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5943341.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14375..15388
                     /gene="argF"
                     /locus_tag="OO332_002933"
     CDS             14375..15388
                     /gene="argF"
                     /locus_tag="OO332_002933"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5943342.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3968 a         3925 c         3969 g         3538 t
ORIGIN      
        1 gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta cgctcctggt
       61 gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc aacgaactac
      121 aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac cccattacgc
      181 gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga tcgcccacaa
      241 aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt agggcctgcc
      301 gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa tgcttgactc
      361 cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa tctacttctg
      421 accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg agttcaaccg
      481 ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat tcaggtatac
      541 ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca cctgcaatct
      601 cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata atcgcggtag
      661 tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact gctttaatgg
      721 gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata gcagtaaaga
      781 gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac ttctgattgc
      841 tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc tgttctacct
      901 taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta cgagcctcat
      961 cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat gcaagcatct
     1021 tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta gggtgaacca
     1081 gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc ttagccgaac
     1141 gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg tgtgggtaca
     1201 aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt gatgtagatt
     1261 gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat actggatttt
     1321 aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc gaaggccgga
     1381 ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc
     1441 acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg cacgccacca
     1501 tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat tacactcagc
     1561 gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac acttcccaaa
     1621 caacaccaga gaaggaccca aaaatgtcga tgataaaaag ctatgccgca aaagaagcgg
     1681 gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa gatgttgaag
     1741 tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat aacgaatggg
     1801 gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgtg gtggcactcg
     1861 ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt ggctggacgg
     1921 cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac tgcgagcaag
     1981 gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt gcggactggc
     2041 aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg ctgttgtgcg
     2101 gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc agccgcgttg
     2161 gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcat gcaatgggat
     2221 gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg gcgatgggtg
     2281 ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg gggcagtttg
     2341 atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt gaggcgctga
     2401 cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct gttccggcct
     2461 ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg ccttatgagc
     2521 tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc gaactgttcc
     2581 cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg cgctaccgag
     2641 tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc acttaaatct
     2701 ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca gccgttgcct
     2761 atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa ttgcccgcac
     2821 gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc tgattactgg
     2881 cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat tgtcggaaat
     2941 cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg cgcaggcagg
     3001 aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg actggcaacc
     3061 gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca cggtgcgggc
     3121 gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca acgatgtgca
     3181 atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt tgctgctcga
     3241 ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat ccttccagaa
     3301 tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat tactgtcgct
     3361 ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca aagactggat
     3421 gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga aactttatca
     3481 gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt atgaacaatt
     3541 gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg aggcacatct
     3601 gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga taaggcttat
     3661 tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata ttccggataa
     3721 tgtgctcggc cagctcggta atcgtgttca gcacgctttg cgagctttta cgccaaaaga
     3781 tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat ttgataccga
     3841 aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg atgcaaaagg
     3901 aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa tggggccggt
     3961 gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca aatatgaaga
     4021 tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg ccagtaccga
     4081 gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg gtattcttgg
     4141 tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga aagatggtgt
     4201 ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg tacgcgggat
     4261 gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt gcccctcacc
     4321 ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac tttgtcaaca
     4381 ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa cagccacagg
     4441 ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg gatgccgtaa
     4501 accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc gaagctgata
     4561 cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat aaacgacagc
     4621 gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca catgttgagc
     4681 tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt gtgcaaacca
     4741 ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg cgtgaggtcg
     4801 gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt ggtcagatca
     4861 gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt cgcggtagcg
     4921 gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact gatgccacct
     4981 aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc tttcgcccat
     5041 aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg gtagttacgc
     5101 gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag caagaccagt
     5161 ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc agaagcctgc
     5221 atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc cccaagaaga
     5281 gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt atacattcct
     5341 gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac aaatttgata
     5401 atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa aatagtttta
     5461 ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta cgcgaaaaac
     5521 tggcgcgcag gcggcggatc ggcacggtgt cccaaaggtt gagaacaatc gccaaagcca
     5581 gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct ttaccgccgt
     5641 tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg gctggcagca
     5701 tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc ataagtgcca
     5761 tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga gcacgatcgg
     5821 tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg tcgagcgcca
     5881 ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg aaatcacgta
     5941 acagtgcagt gccttcgaag cgtgttccct ggttgagagt gacgacctgg gagccgtcgc
     6001 gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca ttaccttttg
     6061 gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt tcgatgaaca
     6121 gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc gccatgccag
     6181 ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac ggtcccgccc
     6241 acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt gccgctttca
     6301 ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt tcggtataca
     6361 gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata agctgcgcca
     6421 tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata tcgccgtcaa
     6481 ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc aagatgaaga
     6541 gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt atcactttaa
     6601 atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc ttaaacgtca
     6661 tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg gattcacctc
     6721 tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac gaagtcatcg
     6781 caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg ccgttgtctt
     6841 taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc ggagaaacag
     6901 cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc gattgcagaa
     6961 cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg cgaactggaa
     7021 ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc cgagcgaatt
     7081 ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa gcaggttatt
     7141 cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg ctttctgact
     7201 gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt cgagacagca
     7261 aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc gcgtcgtccg
     7321 ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg tgagcgcgcg
     7381 atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct cggtaatatg
     7441 ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct ggctgacagc
     7501 tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga gctgggaatg
     7561 cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc ggtgattgag
     7621 tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa aggtttaacc
     7681 ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat gaagtacgat
     7741 atgtgcggcg cggcggcggt ttacggcgtg atgcgtatgg tcgcggagct gcaactgccg
     7801 attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg tgcctatcgt
     7861 ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa caccgatgcc
     7921 gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga gccggaagca
     7981 gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca tcatattact
     8041 ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc tgaacaatcc
     8101 ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct ggagtccaat
     8161 tttgccgata tggcgaacat tggcggtcgt actggtgggg cgattaccgc aggttgcttc
     8221 ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg aaccgcctgg
     8281 cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc acagttcctg
     8341 ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc accctaaccc
     8401 tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc atctttcagg
     8461 gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga aaaacgcaac
     8521 gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg agcaactggt
     8581 gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg cctgtgaaga
     8641 tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag aaagctttgt
     8701 tccgcataat ttagcgggag aaggaccgcg cggcggtgcg ccggtggaga tcgcctggcc
     8761 gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa gctttgcaga
     8821 ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt ctctgaaaca
     8881 actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga atacggcaac
     8941 ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct ttacgagcac
     9001 tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag tttctgcatc
     9061 atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt ccagcaaacc
     9121 atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct gtggcaggtc
     9181 ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat tgccgcagaa
     9241 gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat ctgggaatgg
     9301 aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa ctccgtcgac
     9361 tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa agaagttttc
     9421 gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa ctgggacccg
     9481 aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa aggttcgatg
     9541 tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa agattatctg
     9601 gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc tgttaacccg
     9661 gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct ggttaaccgt
     9721 cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg ctgcgtgaag
     9781 atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc cctgccgatg
     9841 atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt cgataccaaa
     9901 ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa actcgagcgt
     9961 tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct ggaagaaatt
    10021 aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat cgaaccgatg
    10081 ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt tgaagcggtt
    10141 gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt ctcctggatg
    10201 cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg tatcccggca
    10261 tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt gcgcaaggaa
    10321 aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga cacctggttc
    10381 tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga cgccctgcgt
    10441 cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt ctggattgcc
    10501 cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc gcaggtgccg
    10561 ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa gatgtccaaa
    10621 tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct gccagaactg
    10681 ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat ccgtaagcgc
    10741 accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct gcgcttcacc
    10801 ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg tctggaaggc
    10861 taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat gaacacagaa
    10921 ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga ccgctggatt
    10981 ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag cttccgcttc
    11041 gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga ctggtatctg
    11101 gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg tactcgccat
    11161 acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat tccgttcatc
    11221 accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga caccatcatg
    11281 ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact ggccgacacc
    11341 gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat gaacatcgcg
    11401 ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga acgtcgcgta
    11461 aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac cgtgctgcct
    11521 gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga gctgctgatc
    11581 ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa agaagtggcg
    11641 aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg ctttgttgcc
    11701 cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta tgcagaagcg
    11761 aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac acagtgatga
    11821 aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg taaagaaggc
    11881 atgactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc tttattgatt
    11941 aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca gtcaatcatc
    12001 catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac caccaacgca
    12061 cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat acgcccatca
    12121 ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata actggtgcta
    12181 acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg catttccata
    12241 aattgctgta aattctcaat atcatcattt gcatacccag aactatcata ctcatttaat
    12301 atttgatcga agataatata accagcaaca gcgagaaaag gtaataaatc taaaaatgtg
    12361 aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg atgtataccg
    12421 aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc attaaaaata
    12481 ccaatggaaa caataccaac tattgccatc agaaccacag atataataac actacttgaa
    12541 ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc aatggccatt
    12601 aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt aaaactaaat
    12661 cttacgctgt tgagactggt cattaaggcc tgataacgca gtccttttgc tgccataaat
    12721 ataagcaaaa ccaacagtaa taaagtcaaa acacagccaa caagcggcat acctgctgac
    12781 actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac aaaaacattc
    12841 ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc ataaagataa
    12901 cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat cgttaacaac
    12961 acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac aaaagaatgg
    13021 gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat taaaaaatcc
    13081 attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa tactcttatc
    13141 agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt aagaagagag
    13201 tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg aataacattg
    13261 cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct atcgccagcg
    13321 tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac accgttgccg
    13381 atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct tattgggtgg
    13441 ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca ggtagtgaag
    13501 cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt aaagggctag
    13561 cgaaaaaact ggccttaatg gcgatggagc aggctcgaga gatgggcttc aaacgctgct
    13621 atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat ttgggctttg
    13681 agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg cggatgctac
    13741 gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc aattaattag
    13801 tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc ttcgccgtta
    13861 ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc cgccagcgtc
    13921 atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc gctgaggatg
    13981 tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc ggtcacttcg
    14041 taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc gtctgcggaa
    14101 agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag taattcttca
    14161 ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc catggctcat
    14221 tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg cctccacctt
    14281 tgtgacaaag atttatgctt tagacttgca aatgaataat catacacata aatttaattt
    14341 taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc ataagcattt
    14401 cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt tagccgcgaa
    14461 gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta aaaacatcgc
    14521 gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg ccgcatatga
    14581 ccagggtgct cgcgttactt atcttggccc aagcggtagt cagattggtc ataaagagtc
    14641 gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt atcgcggcta
    14701 tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat ggaacggcct
    14761 gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc aggagcattt
    14821 acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac gtaacaacat
    14881 gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc tggtcgcgcc
    14941 acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg cacaacaaaa
    15001 tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg ctgactttat
    15061 ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg aacgcattgc
    15121 attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta acccggaggt
    15181 caaattcctc cactgcctgc ccgcgtttca tgacgaccaa acgacgcttg gcaaaaaaat
    15241 ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct tcgagtctgc
    15301 cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag cggtgatggt
    15361 cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJTRQ010000094        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000094 DAJTRQ010000000
VERSION     DAJTRQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..403)
                     /locus_tag="OO332_002934"
     CDS             complement(<1..403)
                     /locus_tag="OO332_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5943343.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLV"
     gene            complement(390..1412)
                     /locus_tag="OO332_002935"
     CDS             complement(390..1412)
                     /locus_tag="OO332_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5943344.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1426..2928)
                     /locus_tag="OO332_002936"
     CDS             complement(1426..2928)
                     /locus_tag="OO332_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5943345.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3068..4025)
                     /gene="ytfQ"
                     /locus_tag="OO332_002937"
                     /pseudo
     CDS             complement(3068..4025)
                     /gene="ytfQ"
                     /locus_tag="OO332_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4335..4865
                     /gene="ppa"
                     /locus_tag="OO332_002938"
     CDS             4335..4865
                     /gene="ppa"
                     /locus_tag="OO332_002938"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5943346.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5245..5586)
                     /locus_tag="OO332_002939"
     CDS             complement(5245..5586)
                     /locus_tag="OO332_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5943347.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5589..9368)
                     /gene="tamB"
                     /locus_tag="OO332_002940"
     CDS             complement(5589..9368)
                     /gene="tamB"
                     /locus_tag="OO332_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5943348.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9365..11098)
                     /gene="tamA"
                     /locus_tag="OO332_002941"
     CDS             complement(9365..11098)
                     /gene="tamA"
                     /locus_tag="OO332_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5943349.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11304..11942
                     /gene="msrA"
                     /locus_tag="OO332_002942"
     CDS             11304..11942
                     /gene="msrA"
                     /locus_tag="OO332_002942"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5943350.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12265..13608
                     /locus_tag="OO332_002943"
     CDS             12265..13608
                     /locus_tag="OO332_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5943351.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13670..13876)
                     /locus_tag="OO332_002944"
     CDS             complement(13670..13876)
                     /locus_tag="OO332_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5943352.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14201..14755
                     /locus_tag="OO332_002945"
     CDS             14201..14755
                     /locus_tag="OO332_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5943353.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(14818..>15344)
                     /locus_tag="OO332_002946"
     CDS             complement(14818..>15344)
                     /locus_tag="OO332_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943354.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3635 a         4190 c         3778 g         3741 t
ORIGIN      
        1 aaacgagata gctgacgccg cgcaaaaaga acatccccgc aagcgtaatg ataaatgccg
       61 ggatcttcag ggcgtcgatc agaagcccca taaatgcgcc gaaggcacag cccatcacca
      121 gcactagcgg aaacgccagc agcggcgaga ggccgaaatc gccaaccact tttgccagaa
      181 acacgccagt aaaggcgatc accgaaccga cggagagatc gataccgcca gagaggatca
      241 caaaggtcat gccaacggcg attatcccaa gaaaggcgtt atcggtcagg atattgcaga
      301 tcacccttgt ggaagcaaaa ccgggaaact gggtcaggca gtagagatag cccagcacaa
      361 agacgccgat ggtgatcatc agcggcaagt tacgttttat catggctacg tactccttta
      421 atcagactga taaagcgttg cgactgaaca atcagcacac aaagcaccac caccgctttc
      481 accaccaggt tcatctccgg cggaaagccc gaaagcaaaa ttccggtgtt catcccctga
      541 ataatcagcg cccccactac cgagagcagc aggttaaagc gcccgcccat cagcgatccg
      601 ccgccaatca ccaccgcgag aatggcgtcc agctccagcc ataatccggc gttgttggca
      661 tcggcaccgc aaatatccgc cgccacgata atgcccgcaa tcgccgcaca tagcccgctc
      721 aacacgtagg tgagcatgac gatgattcgc gtgtttaccc cggcattttt tgccgcccga
      781 atgttgatac caacgacttc gataaacatc cccagcgccg ttttgcgggt caacagccag
      841 aacaggagaa gcgtcagcac cgcgataatg accggcgttg gcaggaacaa cagcgatccg
      901 ctgccaaacc atgagagatc cggcgagtta aacgtgacga tctgcccggc ggtgatcagt
      961 tgcgccacgc cgcgcccggc gaccatcagg atcagggtgg cgacaaacgg ctgaattttg
     1021 aggatcgcta ccagtatgcc gttccacaat cccgccagga tgccggtgcc cagggcgctt
     1081 aacaaaacaa tcggcaggct gaatcccgcg accgtcatcg cagccgttgt ggctccggcg
     1141 atagccatca ccgcccccac agagagatcg ataccaccgg tggcgatcac cagcgtcata
     1201 ccaatcgcca gtaacgcaac gggagctgca cggttaagaa tgtcgatagg gctaccgaac
     1261 aaacgcccat cctgaagcac cacctgccag aaatgcgggg ctaccaggct atcgaccagc
     1321 agcaccagca acagtgccgc cagctgcggc atccccgtcg gccagtgaaa acgccttttt
     1381 ggcggcgtag tgtcagggag agattgaggc atcacactgt tctccttacg ccgcaatggc
     1441 gttcatgatc gccggaacgg aaagctgtgc cagcgggatc tccgccacct gtttgcgatc
     1501 gcgcatgatg atcacccggt cggcatagcc caccagctct tccagttcgg aggagatcac
     1561 cagcagcgcc agaccgtcag cacacagcgt ttcaatcagg cggatgatct cggcgtgggc
     1621 accaacatca atgccacggg ttagctcatc aaggatcaga aattgcggtc gggtcagtag
     1681 ccaacgtgaa agcagcactt tttgctgatt gccaccggag agaaattcaa tcggttgttc
     1741 agttgaaggt gtgcgaatgc caagctggcg gataaagcgt tcggcaatct cttgctgctc
     1801 tttgcgggaa atgggtcgta gccagccgcg ctgggcctgg agagcaagga tgatattttc
     1861 ccgcaccgag gcggcggcga tgatgccatc ggttttcctg tcttccgggc agaatccgat
     1921 gcccagtact gaagcctgat gtggcgatcg cagggtttgc ggtttgcctt tgatcaacgc
     1981 cgtgccgctg tcagcaggtt tgataccgaa gatcacttcg gcggtttcgg tacgtccgga
     2041 tcccagcaag ccagccagac cgacgatctc gccggggcgc acttcgagat caaacggtgc
     2101 tatcgttcct tttttgccgt aatttttgaa cgcggcaacg ggtttgtcgc tcaacaatgt
     2161 tcgcccggca cgctgtagcg cgtgggtatc cagctcgcgc cccagcatca tttttaccag
     2221 ttcgatctgc ggtagctcgc gcgtttcccg acagcctacg aaactgccgt tgcgtaagac
     2281 ggtgatccga tcgctgacct gatagacctg atcgagaaag tgagtgacaa agatcaggct
     2341 gacgccgcga tcgcgcaact gacgcatcag gccaaataat agctccactt cttgggtgtt
     2401 gaggctggcg gtgggttcat cgaggatcag cactttggcg gagagatcga tagcccggca
     2461 aatggcgacg atttgctgca tcgccaccga aaaacggtta agcggttcgc gcatgtcgag
     2521 ggagaaaccg taagatgcca tcagttcggt ggcgcgcttt tccatctctt tacggcgtag
     2581 aaggccgaaa cgtttgggtt cgcgacctat gaatagatta tcagcaaccg acatattggg
     2641 tagcaggttg acttcctgat agacggtgcc gataccgagt tgttgtgcat gggcggtatt
     2701 tttcggtgag atagtctggc cttccagcca gatggtgccg cgatcggcgt ggtatacacc
     2761 agttaatgct ttgattagcg ttgattttcc cgccccgttt tcaccaagca gcgccatgat
     2821 ttcgccacgg cgcaggctga aatcaacgtt gtctaacgct ttgacgccgg ggaaaaattt
     2881 acttaatcct tcggtgcgga ggatctcctg gtgttggtcg gtcgtcatgg tttcccccgg
     2941 tgtaaagcgt gctcccagta ggcatgataa gacgcggtaa gcgtcgcatc aggcttcagt
     3001 gcaccatcgc cggatgcggc gcgagcgcct tatccggcct acgaatcggc atcaggcata
     3061 gcaaccctca ataccccata tttttcttct tctctaactc ttcttttgcg gtatcaggca
     3121 ggtaaagggt ggatttggtt aacgtcagct tttcaggcat ggtgccgtct tttttgtatt
     3181 tctccagcgc atcgaaagcg ggtcccgcca tgttcggcgt cagttcaaca ctggcgttcg
     3241 cttcgccatc catcatcgct ttgtagatgt ccggtacacc gtcaatggaa cccgtgagga
     3301 tatctttgcc cggtttcagg cccgcttctt taattgcctg aattgcacca atcaccatgt
     3361 cgtcgttatg ggcgtaaacc atgcagatgt ttttgccgtt gttttccgct ttgataaagc
     3421 tctccatgac ttctttgcct ttactgcggg tgaagtcacc tgactgcgag cggataattt
     3481 tgatatttgg cgcattctta atggcttcgg caaagccttt cttaacggtc aatggcgacg
     3541 ctggccccaa cggtgccctg cagctccacc acgttgcatg gtttgccatt cacttctttt
     3601 accagccagt caccgatcaa cttaccttcg aggatgttgt cggcagtaac ggtggtcata
     3661 tagagagatt tgtctttcac atcgatggaa cggtcgagca agaagaccgg gatttcggca
     3721 tctttcgcct cttttaatac cggttcccaa cccgtcgcaa ccactggagc gataaagatc
     3781 gcatccaccc cttgtgcaac gaaggaacgt accgctttaa tctggttttc ctgcttttgc
     3841 tgaccatcgg caattttcaa cgtaattccg cgtttttcgg cctcactttt cgccacattg
     3901 gtttctgcgg cgcgccagcc ggattccgat ctgacctgcg aaaatcctac ggttaatgga
     3961 gcggccaacg ccatagacga catggctgcc gagactgcag agactacaag taagcgtttc
     4021 cacatagggt tgtcctcgtc ggggtaatta ttattggtga aaagatgttc gcgaaaaaac
     4081 tatagacaat tcgttatgta acggattgcg ttacatcaca cttcaaaaaa gtaaataaaa
     4141 cgttaatcac aagtttgtaa tcgctttcat ctcactatga aaaatgcggc tacggttatg
     4201 gattttcctg ctctgtatac cgtcttaaaa ctggcgaaaa aggaaaatga agacgaaaac
     4261 aagcaaagac attcggcgcg agttggctat aatacttggc acttgtttgc cacatatttt
     4321 taaaggaaac agacatgagc ttactcaacg tccctgcggg taaagatctg ccggaagaca
     4381 tctacgttgt tattgagatc ccggctaacg cagatccgat caaatacgaa atcgacaaag
     4441 agagcggcgc actgttcgtt gaccgcttca tgtccaccgc gatgttctat ccgtgcaact
     4501 acggttacat caaccacacc ctgtctctgg acggtgaccc ggttgacgta ctggtcccga
     4561 ctccgtaccc gctgcagcct ggttctgtga tccgttgccg tccggttggc gttctgaaaa
     4621 tgaccgacga agccggtgaa gatgcgaaac tggttgcggt tccgcacagc aagctgagca
     4681 aagaatacga tcacattaaa gacgtaaacg atctgcctga actgctgaaa gcgcaaatcg
     4741 ctcacttctt cgagcactac aaagacctcg aaaaaggcaa gtgggtgaaa gttgaaggtt
     4801 gggaaaacgc agaagccgct aaagctgaaa tcgttgcttc cttcgagcgc gcaaagaata
     4861 aataagttct tctagcgcaa taaccctgaa cgccgggctt cggttagtaa gggttttttt
     4921 atgcccgcga taaataaact ctctattcca ccatcattat tctcagcggt tgcaaggctt
     4981 gaacggtaag aacaagcaaa cccgaccacc attttgctgt tcatagccac ttgctggaag
     5041 ttagccgacc tcactcatac tcaccgttca aagcgtgccc ccgttttacc cctgcacatt
     5101 tgtgaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggct cggagagaaa
     5161 agcaaaagat ggaaaagtta caaaaaaaaa tcgcgagata catcgcataa aacaacgcca
     5221 cccgaaggag gcgtgctttt attattactt atccctgtct aaccagtcgc cgctttcaat
     5281 tagctttaat ccatcgacgg gtcgttgata aacgtacatc catgcactcc catacggcgt
     5341 ctgaatcaac tggcgcgcgt attcaccgcc cctggtgcgc aaggcatcaa gttcggccag
     5401 cgtggcgttg tcaatacgat aaacttcacc gtgtaccgtt ccgttccccg gaactgcgcc
     5461 tggatagtgg cccaggctat acaactggta gttatcgata ctgaaatcgc ccagtaactg
     5521 ggcattggtc atccagtgac tgttgccttg tttgtggcgt aaactgccgt agacaaatat
     5581 tcgcattgct aaaactcgaa ctgatagagc aaatccagtg cctggtctac accagacacg
     5641 gcttccagat atagcttagg catcaggcga taacgtaacg tgagtgttgc tatagagtca
     5701 aatataccca cgccgtattt cacttgcaga cctggcaata catagccgct gaccactacc
     5761 tgggaggagt cgcctactcc cagggtgtcg agcgctaaat tgcttacgcc aaacgtctcg
     5821 ccgattttac ccacaatctg gccactttgc gcaaccccca gaccaatcag catcgaggtc
     5881 attgccgcac tgtcgctctg atcgctctcc agcccttgtc cacgtagcaa ataagacaat
     5941 gcagcttgtt gcgacatcgc cgggtcagag aagatctccg ctttcggttc gtccgccaga
     6001 ccagtgacgc gaactccggc gattacatcg tcttctgtag catccgggtt acgaatagct
     6061 tcaatattaa gatacggttg atccggcgga ccagagaaca gtaactcacc cttacgcaca
     6121 atcagatcct gaccataggc atggaagcgc ccttcaggga tgttgatctg cccgttcagg
     6181 cccagccctt gtttgtcctg tacgacgtta agatcgcccg tcagccgcgc tttcaggcca
     6241 aaggcgtcaa tgcgcacatt gttgccaacg tggacaatca ggttactgtt aatcggaatc
     6301 gacgccgttt tcggctcttc cggttgcagg ttatcgttaa gcatcaccac atcgctggag
     6361 acgcctactg cgctttccgg cagatcgtgc accacgatgc gcgcccacgg gacatccacg
     6421 cgaccatcga gggtaaacag gtttggtgta gcctcgaata caacatctgg cgatacatcc
     6481 attcgtacca tcggcggcac ggtgatccgc actttactgc ctttcgccgt tactcgtgcc
     6541 cgccagtttt caatttggct ccagtcggca tcaccgttca ggtagatttc accctgctgg
     6601 gtccgtactg taccggcaag cgtcgagcgc ataccgttaa agttgaccgc aagctggctc
     6661 ggctgcatat caaacggcat aaagttgccg tcgatatcca cacccgtaac ctgaagctga
     6721 ccaaacaact gcgggctttg cacatcacca cccagacgca agttggcact caccatcccc
     6781 gctgcttttt ccccacgggt aaagatgggg tttatcatcg caaggttgaa gttacggata
     6841 ttgacgttgc caccaagatt acggcggcct tgcggatcgg tcacctgcac ctgtccatca
     6901 aactggccgt tattggtcag gcggatggtc cagcccaatt cggcacggtt gttacgcaat
     6961 tccgccgtca gattcagtgt ctgaaacgcc accggcagcg ccgcatcgtt gacggtttgc
     7021 gttacctgca cgttacgccc cgaaagggtg atactgccct gcggcagccc ctctttcgtg
     7081 gtgtcccagg caacatccgc tttacccgtg aagataccgc tggcctgagt ggtttctggc
     7141 ataaacggtt tcagcatggc gaggtcgaag cggttgagat tcaccaccgc acgcccttcg
     7201 gcccccgcat cgatagtttg cggcacgcac agttccgcat tcgggttaag ccagcagtgt
     7261 ggcccgatgc tgattttttg ctccttattg cggtaatcca gcgcaatatc gcgggtcagc
     7321 gaccacgggc cgaccggcgt ctggaagcgg gtattgctaa gagttccctt ccagcgttct
     7381 tctttgcgat caaaacttcc tgccagatta agctgcccgg agacaggctc gccctgaatc
     7441 cgcaactgta gctcgtgctg cttttcgctg cctttggcat tcagggtgac gaggttgata
     7501 tttacatccg gctgcgaaat ttgctcaacg cgtacgtcga gtttcccggc gatctgatcg
     7561 gtggatttaa tgtcgccttc cacgcgaacc tgcgccacgg aaagttcctg ccagcgcagg
     7621 ccgcgcgcgg tgatatctgc cagtagttgt ggcgcttcca ccgtgccgcg tactttcacc
     7681 agccctttcg ctgtaccgcc aagccccggc agcgcgttat ccagccccgg cgcgttgatg
     7741 gtggcatcaa gattgagatc ttttaccccc agctcgcctt tcacttcggc actgtttggc
     7801 ccgagttcca gatgaagccc agggatcatc cactgcatat aactgttgcc tttcagcgtg
     7861 ccgtcaacgt tcactttgtt ctgtttgacg ttaccggtca gcttcagttc tggcacctcc
     7921 atctgccagg tgccgccgta caggctaccg cgggttttaa tcaagccatt gagtttcgac
     7981 ggccactccg ggaactcttt ggcggtgtaa atgccattaa gcgttagctc accgcgccaa
     8041 ctaatggcct gctgccagtc gagcaacgcc ttgagttcag ttttcccttc cagcgccgcg
     8101 acggtgagtt tgtcgagatt gacctgctgt tcattacctt tggcatcaag ggtaatggtg
     8161 gctggcggga tctcctgtcc cttcactgcc gtacgcatag agagcgtgta atcggtcatt
     8221 ttgccggtaa gtttcagttt cagatcatcc gcctgatact gcttctcacc agtgaacggc
     8281 cagtaaagct gtttgctgtt cacttccacg ttgagtggca atccggcttc cgccagtcgc
     8341 gtctgggcgc gtaaatccat atccaccgga ccggaaaggt taacgcctat ctccagctgt
     8401 tcgcgcagcg cgccgcccac tttcagcttc accttttcac ctttcaacgg ctccacgttc
     8461 agtgtgctgt tgagggtgat atccaccggc cagttgtctg acagctgcgc cgtgccgctg
     8521 gcgttgacga tcccctggtt ggaatcgata tccaaggcgt ccagtttagt attgccgtca
     8581 atgctgctca ctttcagcag catggtgcgc acggtgatgt ccgtgtcgcc cgtcacgcgc
     8641 agctgctcgc ccttaaactc ttcaatgttc aggttaagcg gcaaatgcac gtcggtcatt
     8701 tccggcaata ccgggcgaga aaagagatct ttcagcgttt cgccgagcgg cttttcctcc
     8761 ggctgcggat tttcaatttt cggttcgacc acttcttcct gcgccacttc caccactttc
     8821 ggcagagcaa tcagcaggcc tttcagcgac gtcggtttca gggtcagggt tttctcctgc
     8881 cagttcaggc cggaggtgaa gtccatcacc gacaccgtgg tgtcatcaat cttgatgttg
     8941 acgttgtcca gcgccacccg tgtcagggtg atgggatacg gcgtggagag atccagcgga
     9001 ccgctatctt cctcttcttc aacctgttca gaaggaggca tttttttact gtcgatgttg
     9061 acctgaatgt ctttcagcgc caggtcatta atacaaacgc tactgttcca caggcactca
     9121 agaccgaccg ccagatgcag attacccgct tttaccgcca cgcctagctg ctcataacga
     9181 acgtcagaca aggtgagatc gcgccagccg ccggtgacct tgccaatatc cagtcctggc
     9241 acccagcgat ccgccgcttt aaatacaaga tgcaggccgc tggtggtgcc caccagaaac
     9301 gccaccgatc ccagcaacag taagataacg ataaccacgc cgaggctgat ttttttccat
     9361 aaactcataa ttctggcccc agaccgatgt aaaactgtaa cccgtgttcg tctttatccg
     9421 cgaccggtac ggcaaaatcg agtttgattg gcccgaccgg cgattcccag cgtacgccga
     9481 ccccggtacc ggttttaaag tcgctgcggc gaatatcgct taccgcttcg ccactatcga
     9541 caaacaccgc gccccaccat tttccggtca cgttgtactg gtattccagc gatccggtta
     9601 tcaacttcga ggcccctttc aggtcaccgt tggcgtattt cggggcgata gatttgtatt
     9661 tgtagccgcg aatactgcga tcgcccccga cgaagaaacg cagatccggc ggtactttgt
     9721 cgaaatcacc ggtttcaatc cagcccagcg taccgcgtgt cacaaagcga tggcgatcgt
     9781 acagtgtgcg gatccagacg ttctgcgcct ggaaaacgga gaaatcgaca tctgaacccc
     9841 aggccgtgtt ggagtagtcg atagagtagc gttgcgagtc tccccaggtt ggcatcaggc
     9901 caccacgaga acgcgtgcgg ctaatcatca ccccaggata aaacagcatc gtggtgttgg
     9961 taatttcacc ctgggtaaag tggtcgaggc tccagcgcag gttaatggca cgctgccagc
    10021 cgctggagag atcccagtag cgagaagcca ccagcgtagt ggagtcagat tcggtatcgt
    10081 tcaggtcagt gcgcttaaaa ccgccctgca ccaaataata ttgttccagt ggattcttca
    10141 gcagcggcat tttatagctg aagtcgaggg tctgttccgg cgcggaaata ctggtactgg
    10201 tggtcaaact gtgaccgtat gagttcatcc acggcttttt ccacgtcgct ttcacgcgcg
    10261 gtcccacgtc cgtagagtaa ccgcccccgg tttcgatggt gttttctgtt cgcggcgaaa
    10321 ccacgcccgt caatggtaat actttcgttt cgcgcgcttt atcaaattgt ggagccacca
    10381 ccaccgagtt aaaccagccg gtagcagaaa gtcggcggtt cagttccgcc aaatctttcg
    10441 attcatactc atcgccctct ttaaacggca ccagattttg caggtattca tcgcggattt
    10501 gtgatccttc aaaggtcaca tgcccaaagc ggtaacgttc gccactgtta taatcaatat
    10561 cccagaaggc tttatgcagg ccgagcgcaa tgcccagctg cgctttggta aattcgctat
    10621 cgaaataacc tttacgcaac gcaatactgg ttaaggactt tttgaaattt tcataatcgc
    10681 cctggttcag taccgtacca atagccgggc gagtatcgag caatttcaaa tagtctttat
    10741 cggtccgcgc gccgccgcgc aataccacat cggtgccgcc aattaacacc ggcacacctg
    10801 gcgtgacttt ggcgatcaat acctgccgcc ctttctttgg cggtggacgg agatcaaatt
    10861 caatggtcgg ctggtaataa cccagcgctt tcagaccttc acggatggca tcatcgacgc
    10921 gtgcgcgaaa gcgacggtct ggcgtcactt catcactttc aatcgtagaa agctgcgcac
    10981 gaacgttctt ttccagctgt cccgataacc cctcgacctg tagacggacg ttcgcggcga
    11041 cggcagatcc gcttaagcag agtaagctta cacagcataa ctgtcggata tagcgcacat
    11101 tttctcctga atatcctttt ttcctgcccc tggaacgccg ttaaacggct taacaaaaat
    11161 ccagtaatat ggattaaaaa gcagactaaa ccccaaatat ttcttatgtt ttactttaga
    11221 cctattcacg gtggttattg tgtgcaaata cgcctcttgt tacaacctta accccaatga
    11281 ccgattttcg ggagagcgac accatgagtt tatttgataa aaagcatctg gtttcccccg
    11341 ccgatgccct gcctggacgt aacaccccga tgcccgtagc cacgctgcat gcggtcaacg
    11401 gtcactcaat gaccaatgta cctgacggaa tggagattgc catttttgcg atgggttgtt
    11461 tctggggtgt ggagcgtctg ttctggcagt tacccggcct ttacagcacc gccgcaggct
    11521 ataccggcgg ctatacgcca aatccgactt atcgggaagt gtgctccggt gatacgggtc
    11581 atgccgaagc ggtacgcatt gtttacgatc cttccgtcat cagctatgag cagttgctac
    11641 aggtattttg ggagaatcac gatcccgccc agggaatgcg tcagggcaat gaccacggca
    11701 cgcagtatcg ttcagcgatt tatccgctga ccccagaaca ggatgccgca gctcgcgcca
    11761 gtctggaacg ttttcaggcg gcgatgcttg ccgccgatga cgatcgtcac atcaccacgg
    11821 aaatcgctaa cgccacaccg ttttattatg ccgaagatga ccaccagcaa tatctgcata
    11881 aaaacccata tggttactgt ggaattggcg gaattggcgt ctgtctgccg ccggaagcat
    11941 agcgttacgg gtacaaatgt agtttgttga taaagtgctc tttgtctatg cctgatgcgg
    12001 cttaaacgac ttaccaggcc tacaaaatcg tccaaattca atatattgca aggactgcgt
    12061 aggcctgatc ggcatagcgc atcaggcagt attgcattta tcagcagtct gaattttaac
    12121 cctctggcga ctttacagta ccttacgcta tactagcgac tgaaaatgcc ggttcacttt
    12181 cttcgaatcg gctttcaatg tgtatttcac acaaattaat caactttccc ttccgaggat
    12241 ctggcctgaa aggtcggata agatatgtta aacagtattt tagtcatact ctgcttgatc
    12301 gctgtaagtg cgttcttctc gatgtccgag atctcgcttg ccgcctcacg caaaatcaaa
    12361 cttaaactgc tggctgatga aggcaatata aatgcccaac gcgttctgaa tatgcaggaa
    12421 aatcccggca tgttctttac cgtggtccaa atcggtctga acgcagtggc gattctcggc
    12481 ggtatcgtcg gtgatgcggc attttctcca gcttttcaca gcctgttctc ccgctatatg
    12541 tcggcagagc tctctgagca actgagcttt attctctctt tctcgttagt gactggcatg
    12601 tttatcctgt ttgcggattt aaccccgaaa cgcatcggta tgattgcgcc agaagcggtg
    12661 gctttgcgta tcatcaaccc gatgcgcttc tgcctgtacg tttgcacccc gctggtgtgg
    12721 ttcttcaacg gcctggcgaa catgatcttc cgtattttca aactgccaat ggtacgtaaa
    12781 gatgacatca cttctgatga catctacgcg gtagtggaag ccggtgcgct ggcgggcgtg
    12841 ttacgtaaac aggaacacga gctgattgaa aacgtctttg agctggaatc ccgtaccgtt
    12901 ccgtcttcaa tgacaccgcg tgaaaacgtg atttggtttg atctccacga agatgagcaa
    12961 agcctgaaga ataaggtggc ggaacatccg cactctaagt tcctcgtctg taatgaagat
    13021 attgaccaca tcatcggtta tgtcgattct aaagacctgc tgaaccgcgt gctggctaac
    13081 caaagcctgg cactgaacag cggcgtacaa attcgcaaca cgctgattgt gccggatacg
    13141 ttaacccttt cagaggcgtt ggaaagtttt aaaaccgcag gtgaagactt cgcggtgatc
    13201 atgaacgagt acgcgctggt ggtggggatc atcaccctca acgacgtgat gaccacgctg
    13261 atgggcgatc tggtcggtca ggggctggaa gagcagattg tcgcccgtga tgagaactca
    13321 tggctgattg acggcggcac cccaattgac gacgtcatgc gcgtgctgga tattgacgag
    13381 ttcccgcagt cgggcaacta cgaaaccatc ggcggcttta tgatgtttat gctgcgtaag
    13441 atcccgaaac gcaccgattc ggtgaaattc gccggctaca aatttgaagt ggtggatatc
    13501 gataactacc gcatcgacca gctgctggtg acccggatcg acagcaaggc caccgccctt
    13561 tcgccaaaac tgcctgacgc taaagataaa gaagaaagcg tcgcgtaacc cccagaaaca
    13621 tcaacggctc ctgaatcagg agccgtctta ttactgcata gcactttggt taagccatct
    13681 ctgtttgcag acgcataacc tgacggttaa cttcggacat cactgacaaa tgcagcttat
    13741 ccttcacttt tgggataaga atcttaccct tatcaaattc aaaagcgcca acgtccttga
    13801 tgtataaccg tccacggaac aggatcttta cgtacttcgc cacctgaagt gggttgtacc
    13861 gttggaaaat tttcattgtt atctcctgct gagtattacg cctgtgcggt gccacaatcg
    13921 gcccaactat tatgaggcgc aaattttaat gcttagtgac tatagactat ccgggcaatg
    13981 tttccaccgc gtataacttt ttttacctaa aggttacaat tattcagaat tatcttttta
    14041 ccgaagcgcg gtcttcagta taagcattca tttttcatat gatttgtgcg cttgaccgca
    14101 aactggcatc acacttgcgg gaaattcgat aaatagcaca tatgattaaa actcagaccc
    14161 aagtggtcgg atcacctgca tatcataaga aggaaacacc atgaccctac gcaagattct
    14221 ggcactcacc tgcctgctgt tgccgatgat ggcttccgca catcagttcg aaatcggtca
    14281 gcgagtgccg ccgattggca ttaccgatcg gggcgagttg gtgcttgata aagatcagtt
    14341 tagctacaaa acctggaaca gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat
    14401 tgctggtcgc acctctgcaa aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc
    14461 gaagttaccg cacgatcgtt accagaccac caccattgtt aacaccgacg acgcgattcc
    14521 gggttccggc atgtttgtgc gcagcagtct ggagagtaat aaaaagcttt atcccaggtc
    14581 gcagtttatt gtcgatagca atggcgtcgc gcgcggtgcc tggcagctgg atgacgaaag
    14641 ttccgctgta gtggtgctgg ataaagacgg tcgcgtgcaa tgggccaaag acggggcgct
    14701 cacaccggaa gaggtgcagc aagtgatgga cctgctgcaa aagttgctca aataatggca
    14761 ataacataaa ttctggaaaa ataattttcc cctatcgtta tctatgggat aggggaatta
    14821 agtctctgag ttcttacacc gaactaatac tggtaacgct agttgctctt ttttaaacgc
    14881 ctcaatgatc gatttatatt tctcgcaaat cttttctttg gtaatattac tatctgcggc
    14941 cagattcgca cttactatcg catccacttg ttcggctaat tcgacttgtt ggcaacctgc
    15001 attaaccaga aaattataaa taagagagta atcttcatcc tccaaaacaa cagaggaacc
    15061 attacgaaaa cgttcatccc ctccatagat tttaagcgca aagcccaaag attctatgac
    15121 tatggcaaaa ttatcatcaa ttattttttc gtaaatatac catccatcac attctttaat
    15181 tccaccgcct ggtatttgga gttcaaattt accccgtttt aatttgctta ctacatcctc
    15241 caataaggca taaccgaaag atacgtcacc aaacgcctgg cgaatataac ctttaatcct
    15301 ttttggtatg tcagactgtt ttttctgcaa aatatcacag gcat
//
LOCUS       DAJTRQ010000095        15037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000095 DAJTRQ010000000
VERSION     DAJTRQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            2..238
                     /locus_tag="OO332_002947"
     CDS             2..238
                     /locus_tag="OO332_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5943355.1"
                     /translation="MLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAVLAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO332_002948"
     CDS             248..886
                     /locus_tag="OO332_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5943356.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO332_002949"
     CDS             889..2052
                     /locus_tag="OO332_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5943357.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..3760
                     /locus_tag="OO332_002950"
                     /pseudo
     CDS             2136..3760
                     /locus_tag="OO332_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="OO332_002951"
     CDS             complement(3877..4152)
                     /gene="yjfN"
                     /locus_tag="OO332_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5943358.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="OO332_002952"
     CDS             complement(4301..4630)
                     /gene="bsmA"
                     /locus_tag="OO332_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5943359.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4812..5561
                     /gene="yjfP"
                     /locus_tag="OO332_002953"
     CDS             4812..5561
                     /gene="yjfP"
                     /locus_tag="OO332_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5943360.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="OO332_002954"
     CDS             complement(5558..6313)
                     /gene="ulaR"
                     /locus_tag="OO332_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5943361.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="OO332_002955"
     CDS             complement(6421..7485)
                     /gene="ulaG"
                     /locus_tag="OO332_002955"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5943362.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7840..9237
                     /gene="ulaA"
                     /locus_tag="OO332_002956"
     CDS             7840..9237
                     /gene="ulaA"
                     /locus_tag="OO332_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5943363.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9253..9558
                     /gene="ulaB"
                     /locus_tag="OO332_002957"
     CDS             9253..9558
                     /gene="ulaB"
                     /locus_tag="OO332_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5943364.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9568..10032
                     /gene="ulaC"
                     /locus_tag="OO332_002958"
                     /pseudo
     CDS             9568..10032
                     /gene="ulaC"
                     /locus_tag="OO332_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10046..10696
                     /gene="ulaD"
                     /locus_tag="OO332_002959"
     CDS             10046..10696
                     /gene="ulaD"
                     /locus_tag="OO332_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5943365.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10706..11542
                     /gene="ulaE"
                     /locus_tag="OO332_002960"
     CDS             10706..11542
                     /gene="ulaE"
                     /locus_tag="OO332_002960"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5943366.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11542..12228
                     /gene="ulaF"
                     /locus_tag="OO332_002961"
     CDS             11542..12228
                     /gene="ulaF"
                     /locus_tag="OO332_002961"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5943367.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12357..12632)
                     /locus_tag="OO332_002962"
     CDS             complement(12357..12632)
                     /locus_tag="OO332_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5943368.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12959..13354
                     /gene="rpsF"
                     /locus_tag="OO332_002963"
     CDS             12959..13354
                     /gene="rpsF"
                     /locus_tag="OO332_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5943369.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13361..13675
                     /gene="priB"
                     /locus_tag="OO332_002964"
     CDS             13361..13675
                     /gene="priB"
                     /locus_tag="OO332_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5943370.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13680..13907
                     /gene="rpsR"
                     /locus_tag="OO332_002965"
     CDS             13680..13907
                     /gene="rpsR"
                     /locus_tag="OO332_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5943371.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13949..14398
                     /gene="rplI"
                     /locus_tag="OO332_002966"
     CDS             13949..14398
                     /gene="rplI"
                     /locus_tag="OO332_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5943372.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14469..>15037)
                     /locus_tag="OO332_002967"
     CDS             complement(14469..>15037)
                     /locus_tag="OO332_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5943373.1"
                     /translation="SVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQ
                     DVESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYID
                     QLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDI
                     RSDRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3513 a         3662 c         4051 g         3811 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgtgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacgttttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaggccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcaattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatggc aggttgaggc
     2521 tgggtcgtta tgtccaataa cgatgacctt tgccgccacg ccattgttgt tacagatgtt
     2581 acccacgccg tttcaggact ggaccacgcc gctgctgagc gatcgctacg attctcactt
     2641 attgccaggt gggcaaaaac gcggtttgtt gattggcatg ggaatgacgg aaaagcaggg
     2701 cggttccgat gtcatgagca acaccatccg tgcagagcgt ttggaagatg gctcttatcg
     2761 gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc tggtgctggc
     2821 gcagaccgcg ggcggtctgt cctgcttttt tgtgccgcgc tttttgcctg acgggcaacg
     2881 caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta acgccagttg
     2941 cgaagtggag tttcaggatg ccattggctg gttattgggg caggaagggg aaggaattcg
     3001 tctgatcctg aaaatggggg ggatgacgcg ttttgattgc gccctgggta gccatgccat
     3061 gatgcgccgt gcattttcgc tggcgattta tcatgcacat caacgccatg tttttggtaa
     3121 tccattgatc caacagcccc ttatgcgtca tgtcttaagt cgcatggcac ttcagcttga
     3181 agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgtg ccgatgccaa
     3241 agaagccctg tgggcgcgtt tatttacgcc tgcggcaaaa tttgtgatct gcaaacgagg
     3301 tatgccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt gcgaggagag
     3361 cgagctgccg cggctttacc gggagatgcc ggtaaacagt atttgggaag gttccggcaa
     3421 tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat acgacttatt
     3481 gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg cggttcgtcg
     3541 tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta ctcatcagct
     3601 attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa tggcgcaggc
     3661 gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc agatccagaa
     3721 tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga ctgatgcgac
     3781 gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa ggcgctcgcg
     3841 ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta tcgcctgtac
     3901 gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc atgacgcacc
     3961 ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc ctgaaatatt
     4021 gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac tggcgaattc
     4081 tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg gtgaggcaag
     4141 taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg cattccatcg
     4201 cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa acgcataggg
     4261 acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg tacaaaatgg
     4321 cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca acgtaataat
     4381 cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc ggagaacccc
     4441 gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg cggcgaatct
     4501 cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt gcgctacagg
     4561 cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta ttacgtttcc
     4621 tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc cgaattgtta
     4681 cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc tcaacattat
     4741 gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata ccagacaaaa
     4801 aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc gttcttcatg
     4861 cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt aattttttat cacggcttta
     4921 cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct ggtttgcggg
     4981 tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa gcgcggcggt
     5041 taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact ttacgtgcgg
     5101 caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc gcgtcgatgg
     5161 gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt accgccagca
     5221 tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg ctgatacctg
     5281 aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca gagtgggaag
     5341 cgacaaacca cctagaacaa cttggggaca gacctctact gctgtggcat ggcctcgacg
     5401 atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc gagacgggac
     5461 gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgcatt acgcctgagg
     5521 cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga atgctgaccc
     5581 cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg atcagcatat
     5641 cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta ctgctatcaa
     5701 ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt gctgtcagca
     5761 tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac atccagtgcc
     5821 cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga cttttgttgt
     5881 actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa tttgccagcg
     5941 gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca agcagaaacg
     6001 cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc gaggccgctt
     6061 tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc ggcgtccagc
     6121 gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt ttgccgcttt
     6181 cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga cgctcaacga
     6241 ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt tgatgtcttt
     6301 gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata tttgaaatag
     6361 ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat aaacgacaga
     6421 ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat cgaaaccgcg
     6481 cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc cgccaacctg
     6541 ccagataaac ggcttgaagc catacttcag acgatctttt ttcatctccc acagcacgcg
     6601 gatctcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg ggataaccac
     6661 tttcgcattc agggcttcac ccatacgcag catatcggcg ctggtcattt tgtcggtaat
     6721 accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt cgttaccgtg
     6781 cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac cgccaggggt
     6841 tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc cagccgcttt
     6901 ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg catgaatttc
     6961 aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct ctttcggtac
     7021 gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta cgtcatcagc
     7081 acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt gatcgtgagt
     7141 cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg gggtggtgcg
     7201 taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac cctgtttcat
     7261 taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc aaacgttggt
     7321 gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg caaatgtgcc
     7381 aggggcgacc tgctcttgtt caatttcttc attcagccag ctaccccact ccgggaaagt
     7441 gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca tttaccttca
     7501 tgatagttca attcgaatca atatgtgatt ggttttgatt aatcctgaca ctattttttc
     7561 aggaaggcaa tgaccatttt ttgacttttt gccagggaag ttgttgttga ttttgagtgt
     7621 agaaagattt aatggaatgt atagttaaat taactaaatg aatttaaata aataattttt
     7681 tcgttgtgtg aatcccactc tatccgtgtg gaattatttg cgggtcgcgt cacatttaat
     7741 cataaataat cttgttgtga ttacttttga aaattagagt gagtgcacaa cattccgggt
     7801 gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc
     7861 tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc
     7921 tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa
     7981 accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa
     8041 ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac
     8101 gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg
     8161 gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt
     8221 cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat tgccgttacg
     8281 ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg
     8341 ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc
     8401 tgcggtttct ccatcggtca ccagcaacag tttgcatcat taattgccta taaagttgcg
     8461 ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat
     8521 attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt tggtgccatt
     8581 ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca ctggacggtc
     8641 tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac tcagggtgtg
     8701 cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg cctgatccct
     8761 ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgctccgaa cgccgtggtc
     8821 tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc
     8881 ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa cgccaccatc
     8941 ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg tctggtgatg
     9001 gggatgatcg aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat gagtgcctgg
     9061 atggggatgg cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc
     9121 ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc
     9181 cgcgcactgc gcgcagaaga agatgcagaa aaacaattgg cagaacagtc tgcttaataa
     9241 ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc
     9301 atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta
     9361 aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct
     9421 tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc
     9481 aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc
     9541 ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc ggagaataaa
     9601 tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac
     9661 ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     9721 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     9781 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     9841 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggtggt cgatgccaac
     9901 acgcactagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     9961 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
    10021 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
    10081 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
    10141 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
    10201 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
    10261 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
    10321 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
    10381 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
    10441 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
    10501 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
    10561 ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
    10621 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
    10681 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
    10741 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
    10801 cttcgatttt gtcgagatgt cggtagatga aactgacgag cgcctgtcgc gcctcgactg
    10861 gagccgcgag cagcgtctgg cgctggtgaa tgcgattgtt gaaaccggcg tgcgtgtgcc
    10921 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
    10981 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
    11041 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
    11101 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcac aggtgacgct
    11161 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
    11221 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
    11281 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
    11341 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
    11401 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
    11461 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgca tggccaaagc
    11521 aggcatggtg gaggcggcat aatgcaaaag ctaaaacagc aggtgtttga agccaacatg
    11581 gatctgccgc gctatgggct ggtgaccttt acttggggca acgtcagcgc tatcgaccgc
    11641 gaacgcgggc tggtggtgat caagcccagc ggcgttgcct acgaaaccat gaaagcagac
    11701 gatatggtgg tggtcgatat gagcggcaag gtggtggaag gggagtatcg cccatcttcc
    11761 gacactgcga cgcatctcga actctaccgt cgttacccgt cgcttggcgg cattgtccat
    11821 acccactcca ctcatgccac cgcatgggcg caggcggggc tggcgatccc ggcattaggc
    11881 accacgcacg ctgactactt ctttggtgat atcccgtgta cgcgtggatt aagcgaagaa
    11941 gaggtgcagg gtgagtatga actgaacact ggcaaagtga ttatcgaaac gctgggcaac
    12001 gccgagccgc tgcatacgcc gggaattgtg gtgtatcagc acggaccgtt cgcctggggg
    12061 aaagatgccc acgacgcggt gcataacgcg gtagtgatgg aagaagtggc gaaaatggcg
    12121 tggattgcgc gcagtattaa tccacaactc aatcatatcg acagctacct gatgaataaa
    12181 cacttcatgc gtaagcacgg tcctaacgct tattacgggc agaagtaaaa cacgcgctgc
    12241 ggaaatttcc ttcctcggga gataactggt ctaattccgc agccgttttt caaaaaaagc
    12301 cccctgcgaa gggggcaaag caaactatgg caatgtttcg ttggttatac ctggtgctag
    12361 cgataaatat ccgcgctggc gtgcatattg ccgttactcc ccggttcccg catcagaatt
    12421 acgtggtagt acgttgcgcc ttgcgcatct gtttcttcat ttaatgcctg acgtgcttcg
    12481 ctttcagtgg cgaaattatg attgatataa atcacgccta agctttgcac atcgtccata
    12541 tttctggcct ggtggttatt aatttcaatg gctgcccatg tatttgcact tagcaaaagc
    12601 acagccagaa gggctaaaac tcgactgaac atagatacct cctcgacggc tgactttgtg
    12661 tgctctcctc tgtgatgatc ttctgattta attttaatca atgataaaga agttgatggt
    12721 gaccatttct gatgcagttg ttcaaaaaaa cactgtgacg gaatgtgatg aacttcaaat
    12781 cagcggttta gaggttattt gcgaaagggg agatttattt ctgctccgcc cttgagttta
    12841 gcggggtatg tcagtactat aacgcgtcat ttttcagccg acctttaaca cgttccttgc
    12901 ctccccggga ttcggctgac ccagacagga ggctgaataa tccgtaagga gcaattcgat
    12961 gcgtcattac gaaatcgttt ttatggtcca tcctgaccag agcgaacagg ttccgggcat
    13021 gatcgagcgc tacactgctg ccatcactgg tgcagaaggc aagatccacc gtctggaaga
    13081 ctggggccgc cgtcagctgg cttacccgat caacaaactg cacaaagcac actacgttct
    13141 gatgaacatt gaagctccgc aggaagtgat cgatgagctg gaaactacct tccgcttcaa
    13201 cgatgccgtt atccgcagca tggttatgcg taccaagcac gctgttaccg aagcatctcc
    13261 gatggttaaa gcgaaagacg agcgccgtga gcgtcgcgat gatttcgcaa acgaaaccgc
    13321 tgatgatgct gaagctgggg attctgaaga gtaatttctg atgaccaacc gtctggtgtt
    13381 gtccggcacc gtgtgcagga ctccccttcg aaaggtcagt ccatcaggaa ttcctcactg
    13441 ccagttcgtg cttgagcatc gttctgtgca ggaggaagcc ggctttcacc ggcaggcatg
    13501 gtgtcaaatg cccgttattg ttagcggaca cgaaaaccag gccattactc acagtataac
    13561 ggtcggcagt cgcataaccg ttcaggggtt catttcatgc cacaaggcaa agaacggact
    13621 gagcaaaatg gttttgcacg ccgagcagat tgaattgata gattctggag actagccata
    13681 tggcacgtta tttccgtcgt cgcaagttct gccgtttcac cgcggaaggc gttcaagaga
    13741 tcgactataa agatatcgct acgctgaaaa actacatcac cgaaagcggt aagattgtcc
    13801 caagccgtat caccggtacc cgtgcaaaat accagcgtca gctggctcgc gctatcaaac
    13861 gcgctcgcta cctgtccctg ctgccgtaca ctgatcgcca tcagtaatcg gtcacggtcc
    13921 attaatacga ctttgagagg ataaggtaat gcaagttatt ctgcttgata aagtagcaaa
    13981 cctgggtagc ctgggtgatc aggtaaacgt taaagcgggc tatgctcgta acttcctggt
    14041 accgcagggt aaagctgttc cagctaccaa gaaaaacatt gaattcttcg aagcacgtcg
    14101 cgctgaactg gaagctaaac tggctgaagt tctggcagct gctaatgctc gcgctgagaa
    14161 aatcaatgca ctggaaactg ttaccatcgc gtctaaagct ggcgacgaag gtaaactgtt
    14221 cggttccatc ggtactcgcg acatcgctga cgctgtaact gcagctggcg ttgaagtggc
    14281 taagagcgaa gttcgtctgc cgaacggcgt tctgcgtacc actggcgaac acgaagtgag
    14341 cttccaggtt cacagcgaag tattcgcgaa agtgatcgta aacgtagtag ctgaataatt
    14401 cgttattcaa cgagacgtaa aaacgccgac cattggtcgg cgttttgctt tctatttttc
    14461 gtcaggcatt aatttcgcaa gtagatccat tcaccattct cttgtgtcca taagtcagga
    14521 tggtttgctg gcgttagatg catacgatca ctccttatgt ctcggtcctg cggcactcgc
    14581 tcgaaaccta aacccgagta gattgcggct gaatttgata aggaacgtag accaattttc
    14641 aaatcatggg gtaaatcaga atacgcgttt agcaatgcag caatgatgaa tccgagggca
    14701 ttagaaacgc ctgtaacagt gcgaagttgg tcgatataaa gatcgattgt tggtagttca
    14761 gatgaccagc aattaggcat ttgggcagga agggttacaa cgtatcctat cgtttggccg
    14821 gtatagtctg gagtactata accaggtccc aaaacaacct cgtgtattgt gtatttactg
    14881 ttgggttcga aggattcgac atcttgcaat aaatatttca gttctgcggt taacctcgca
    14941 cccgcgtaag ctttattgta tttctgggcg tgaatattat ctaatgtagc ttcatggttc
    15001 catccaaaag aattatcacc attcattgtt actgaat
//
LOCUS       DAJTRQ010000096        14925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000096 DAJTRQ010000000
VERSION     DAJTRQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            597..794
                     /locus_tag="OO332_002968"
     CDS             597..794
                     /locus_tag="OO332_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943374.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO332_002969"
     CDS             complement(744..1937)
                     /locus_tag="OO332_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943375.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO332_002970"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO332_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5943376.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO332_002971"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO332_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5943377.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO332_002972"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO332_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5943378.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="OO332_002973"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="OO332_002973"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5943379.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="OO332_002974"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="OO332_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5943380.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10552..10695)
                     /locus_tag="OO332_002975"
     CDS             complement(10552..10695)
                     /locus_tag="OO332_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943381.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="OO332_002976"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="OO332_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR5943382.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3554 c         3707 g         3644 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactccaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgttta aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcac aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat
    14881 gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DAJTRQ010000097        14685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000097 DAJTRQ010000000
VERSION     DAJTRQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="OO332_002977"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="OO332_002977"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5943383.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="OO332_002978"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="OO332_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5943384.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="OO332_002979"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="OO332_002979"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5943385.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="OO332_002980"
     CDS             complement(3965..5611)
                     /gene="fumB"
                     /locus_tag="OO332_002980"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5943386.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="OO332_002981"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="OO332_002981"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5943387.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="OO332_002982"
     CDS             7086..8594
                     /locus_tag="OO332_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5943388.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="OO332_002983"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="OO332_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5943389.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..10291)
                     /locus_tag="OO332_002984"
     CDS             complement(10124..10291)
                     /locus_tag="OO332_002984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5943390.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10307..11497)
                     /gene="uidB"
                     /locus_tag="OO332_002985"
     CDS             complement(10307..11497)
                     /gene="uidB"
                     /locus_tag="OO332_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5943391.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="OO332_002986"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="OO332_002986"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5943392.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /gene="uidR"
                     /locus_tag="OO332_002987"
     CDS             complement(13693..14283)
                     /gene="uidR"
                     /locus_tag="OO332_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5943393.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="OO332_002988"
     CDS             complement(14512..>14685)
                     /locus_tag="OO332_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5943394.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3820 c         3495 g         3685 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgtagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagggata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacaaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgctggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatca aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcag gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gcacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DAJTRQ010000098        14640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000098 DAJTRQ010000000
VERSION     DAJTRQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..561)
                     /locus_tag="OO332_002989"
     CDS             complement(<1..561)
                     /locus_tag="OO332_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5943395.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQAESEVSTPVHPSASMVGLDAGVA"
     gene            569..1069
                     /gene="tnpA"
                     /locus_tag="OO332_002990"
     CDS             569..1069
                     /gene="tnpA"
                     /locus_tag="OO332_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5943396.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1108..1455)
                     /gene="grxD"
                     /locus_tag="OO332_002991"
     CDS             complement(1108..1455)
                     /gene="grxD"
                     /locus_tag="OO332_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5943397.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1790..2617
                     /gene="mepH"
                     /locus_tag="OO332_002992"
     CDS             1790..2617
                     /gene="mepH"
                     /locus_tag="OO332_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5943398.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2745..3326
                     /gene="sodB"
                     /locus_tag="OO332_002993"
     CDS             2745..3326
                     /gene="sodB"
                     /locus_tag="OO332_002993"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5943399.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3472..4641)
                     /locus_tag="OO332_002994"
     CDS             complement(3472..4641)
                     /locus_tag="OO332_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943400.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4807..4896)
                     /gene="ynhF"
                     /locus_tag="OO332_002995"
     CDS             complement(4807..4896)
                     /gene="ynhF"
                     /locus_tag="OO332_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5943401.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5195..6220
                     /gene="purR"
                     /locus_tag="OO332_002996"
     CDS             5195..6220
                     /gene="purR"
                     /locus_tag="OO332_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5943402.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6217..7149)
                     /locus_tag="OO332_002997"
     CDS             complement(6217..7149)
                     /locus_tag="OO332_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5943403.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7262..8473
                     /locus_tag="OO332_002998"
     CDS             7262..8473
                     /locus_tag="OO332_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5943404.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8764..9912
                     /gene="cfa"
                     /locus_tag="OO332_002999"
     CDS             8764..9912
                     /gene="cfa"
                     /locus_tag="OO332_002999"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5943405.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9952..10593)
                     /gene="ribE"
                     /locus_tag="OO332_003000"
     CDS             complement(9952..10593)
                     /gene="ribE"
                     /locus_tag="OO332_003000"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5943406.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10808..12181
                     /gene="mdtK"
                     /locus_tag="OO332_003001"
     CDS             10808..12181
                     /gene="mdtK"
                     /locus_tag="OO332_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5943407.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12222..13478)
                     /locus_tag="OO332_003002"
     CDS             complement(12222..13478)
                     /locus_tag="OO332_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5943408.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13786..13862
                     /locus_tag="OO332_003003"
     tRNA            13786..13862
                     /locus_tag="OO332_003003"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13820..13822,aa:Val,seq:gac)
     gene            13867..13943
                     /locus_tag="OO332_003004"
     tRNA            13867..13943
                     /locus_tag="OO332_003004"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13901..13903,aa:Val,seq:gac)
     gene            14051..14356
                     /locus_tag="OO332_003005"
     CDS             14051..14356
                     /locus_tag="OO332_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5943409.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14482..>14640
                     /locus_tag="OO332_003006"
     CDS             14482..>14640
                     /locus_tag="OO332_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5943410.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMM"
BASE COUNT         3576 a         3785 c         3479 g         3800 t
ORIGIN      
        1 agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag ttgatacttc
       61 actttctgcc tgaatactga tgtaccactt accgcaggac tggctgacag tgacattttt
      121 cacaactccc gtgacctgac ggctgttccg gtagcgcatc cagccaagtt tcggcagaaa
      181 aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaatg cagcattctg
      241 tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt tgtaagcccg
      301 ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa gccattgcgt
      361 ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
      421 atttccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa cgaaacgaca
      481 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta actggaattt
      541 aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga
      601 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccag
      661 ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt acttttcaaa
      721 tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
      781 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
      841 catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa
      901 aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc caatatccgt
      961 catccgccaa tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
     1021 tccccgccct gaaggacgag gttttacggc gcaccggata aaggactgac ggatgcccgt
     1081 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
     1141 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
     1201 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
     1261 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
     1321 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
     1381 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
     1441 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
     1501 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
     1561 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
     1621 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
     1681 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
     1741 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
     1801 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
     1861 ggcccatgct tcaaagcaag ccagggagag ttctgctacc gctcatatca ccaaaaaagc
     1921 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc agaaaacagc
     1981 gaaaaaagcc gccagtaaaa gtacgaccaa aagcaaaacc gcttcttccg ttaaaaaatc
     2041 ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca ataaaacggc
     2101 ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc attgtgtgaa
     2161 agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg tgcaaaaagc
     2221 cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc gttggggcgg
     2281 cagctcaccg cgtaccggtt ttgattgcag cggcctggtt tattacgctt ataaagattt
     2341 ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg atgcaggacc
     2401 tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc agggacgcgg
     2461 tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt caccgcgcac
     2521 aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc actatgttgg
     2581 cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt tgttacggca
     2641 acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca tacgcacaat
     2701 aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca ttcgaattac
     2761 ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa actatcgagt
     2821 atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg attaaaggta
     2881 ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt ggcgtattca
     2941 acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca ccgaacgccg
     3001 gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc agctttgccg
     3061 atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc tggacctggc
     3121 tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg ggtactccgc
     3181 tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct tattacatcg
     3241 actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg aactgggaat
     3301 tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg cctgatgcgc
     3361 ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag ataaatcgca
     3421 cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta attagctgtt
     3481 agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca ataatgccag
     3541 tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag cggaaattac
     3601 cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa tattgactga
     3661 tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta acggaggtac
     3721 aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga actcattgcg
     3781 ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc ctttcaacgt
     3841 gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag agaatccgac
     3901 accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa tactttgcag
     3961 taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta ccgtcgtcag
     4021 caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca cttcaggtcg
     4081 tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa tcactcccag
     4141 ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc aggtcgccgc
     4201 cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa ctgcgctggc
     4261 ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa aaaatgctcc
     4321 gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg gcgcgatggc
     4381 ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac tgcggcgggc
     4441 acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa ctgcataggc
     4501 actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa tgacgggcaa
     4561 caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg ccagcgccag
     4621 caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac gcgatattca
     4681 ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa aagagaaaac
     4741 acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg ctccctcgag
     4801 aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga ggacgataat
     4861 cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt ttttattacc
     4921 acacaaaaag tgatattacg catttttaca cactgttatg aaaaaatctc ccgtcattta
     4981 taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa ttccacgctt
     5041 acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt tttccggcgt
     5101 accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct tgcgattttg
     5161 caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag atgtagcgaa
     5221 acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac gtttcgtcgc
     5281 tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact cccctagcgc
     5341 ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg cgaccagcag
     5401 cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct tccagaaagg
     5461 ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg cttatctgtc
     5521 gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt acccagagcc
     5581 gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg actggggtga
     5641 agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg gctacatggc
     5701 cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg gcccgctgga
     5761 acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag aagcgatgat
     5821 caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg gttatcgcgc
     5881 catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg gtggcgatat
     5941 catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc cgcaggatgt
     6001 ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg cgctgaccac
     6061 gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt tggatcgtat
     6121 cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga ttgaacgccg
     6181 ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg cgggagtctc
     6241 ttccggctcc cgcagccact ccttattcag cgtctcacta tcgccgagat actcaagcag
     6301 ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg ccgaatccgg
     6361 gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg gtttggcgaa
     6421 atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg atgattccca
     6481 gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt taggtagcgt
     6541 tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat cgctgaacgg
     6601 gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc ttagcatccc
     6661 catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc caattgccag
     6721 ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct gaaagacaag
     6781 aagttcaaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg tacgttctgg
     6841 cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg ctatctgctg
     6901 acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt tgagaaacca
     6961 cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg gtaccgccag
     7021 ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacgcgat gcagctcctg
     7081 tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga gtgaatattc
     7141 tgaccacata gtcagcctgc aaaatttttg aaaccagtca tcaaatatta ccgtttcaca
     7201 acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt agagaatata
     7261 catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac tcggttttct
     7321 ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc aaacgcctgc
     7381 gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag cccagcttct
     7441 gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg gcctgacaat
     7501 ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc tgctggtatt
     7561 gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag cattagtgac
     7621 agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc cgctggtggg
     7681 tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt tttcctggca
     7741 ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta ttttctggct
     7801 caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta ccgacctgct
     7861 acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag ccagtttttt
     7921 tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc cggcagttat
     7981 tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg gctgtcgcac
     8041 cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc tgtttgctgt
     8101 cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg tcgaaatcct
     8161 gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg ttgtcgccca
     8221 ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga acactctgca
     8281 actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta tcagcacgcc
     8341 attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc tgggttacat
     8401 gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag tcgctcatag
     8461 cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa attttgttgt
     8521 atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat aaatattatg
     8581 tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg caagggttcc
     8641 tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg agatttctca
     8701 caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag tgatggagaa
     8761 actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg gtaccgtatc
     8821 gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc ggcggatatt
     8881 cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt ggggttaggc
     8941 gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt tagcaaagtc
     9001 ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct gcgtattgcc
     9061 ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg caaggagcat
     9121 tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca atattcctgc
     9181 gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct caaaatgatt
     9241 tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg cggctggggc
     9301 ggactggcgc actacatggc atctaactat gacgtaagcg tggtcggcgt caccatttct
     9361 gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac cattttgctg
     9421 caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg gatgttcgag
     9481 cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa tttgaaaccg
     9541 gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa tgttgatccc
     9601 tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca gattgctcaa
     9661 tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga ttacgatact
     9721 acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc ggataactac
     9781 agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg tgctttccgc
     9841 gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa cggccttcga
     9901 gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag atcaggcttc
     9961 tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta ccgtatctac
    10021 cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt ttttcttccc
    10081 aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg ttggcgtgac
    10141 ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca ggatatattt
    10201 catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg aggttaatat
    10261 tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc caatttcatc
    10321 actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat cgccaagatt
    10381 ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt taatttccgt
    10441 cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc cgtccagcat
    10501 gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa tcgacaccag
    10561 ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa tcagttaaga
    10621 cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg gtaagaatgg
    10681 ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt tcccctgctt
    10741 cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac aaataaaagg
    10801 tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag caatcccggt
    10861 gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga tggcgggcgg
    10921 ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc ttccggcgat
    10981 cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat taaatggttc
    11041 cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg caggttttgt
    11101 ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct ccatggaaaa
    11161 catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt tgtggggcgc
    11221 gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg caaaaaccaa
    11281 gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga actatatctt
    11341 tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg tggctactgc
    11401 ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg cccgctccat
    11461 gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgat cctgcggtta tgaaacgact
    11521 gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac tgtttgccgt
    11581 cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc agattgccct
    11641 gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg taactatccg
    11701 cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg cgcggaccgg
    11761 gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt cactgcggga
    11821 gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg cgcatttgat
    11881 gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca gtgggatttt
    11941 gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt actgggtgct
    12001 gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac ctatggggcc
    12061 agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta tgatgatgct
    12121 gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag cgtcccgcta
    12181 ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg ctggagcgtt
    12241 gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt ttgccgactg
    12301 gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt gatgaagtca
    12361 ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg caggcgtttc
    12421 actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc gccattcaga
    12481 ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc gtcgagaatc
    12541 aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc ataaccaata
    12601 atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg tgcgcgatct
    12661 tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac tgttccacct
    12721 tgtattaccg cattttccag cgtaccggca tgcacttcag cccgatcccc cacattcacc
    12781 gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc cagacgataa
    12841 atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt catggtgcca
    12901 ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc ctgattaatc
    12961 tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc atctaccacg
    13021 accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat gattttttcc
    13081 gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa accgtaggca
    13141 taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt agcgagcgta
    13201 gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc accgtcgctg
    13261 accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc ggttgccact
    13321 ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc ttcaacaatc
    13381 aactcgcctt cagtaagttg cgtcgcgcca atgacctcgc cggtcttaac aatttgcaca
    13441 ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt tgctgcatct
    13501 gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac gacagatttg
    13561 agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt tgtatcagat
    13621 gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag ccaatttgct
    13681 taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc ttgccacctt
    13741 tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt catagctcag
    13801 ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa ttgaacgcac
    13861 catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg gggtcggtgg
    13921 ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt ttccctctgt
    13981 ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg ttatcagaag
    14041 gagaatcgct atggcaactt tgttacaatt tcattttgct tttaatggcc catttggcga
    14101 cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac ctggttttct
    14161 gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct accttttcac
    14221 tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga aaaatctcgg
    14281 cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc aaatcaatca
    14341 ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc atccctgtct
    14401 gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta aattcgaaag
    14461 aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg gcctacgggg
    14521 atctacacct tattctcgct tctacagcaa caaactccac tttctatttc tatcttcgag
    14581 caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc aaaaatgatg
//
LOCUS       DAJTRQ010000099        14636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000099 DAJTRQ010000000
VERSION     DAJTRQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            25..480
                     /locus_tag="OO332_003007"
     CDS             25..480
                     /locus_tag="OO332_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943411.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(558..1469)
                     /gene="rdgC"
                     /locus_tag="OO332_003008"
     CDS             complement(558..1469)
                     /gene="rdgC"
                     /locus_tag="OO332_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5943412.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1594..2502
                     /gene="mak"
                     /locus_tag="OO332_003009"
     CDS             1594..2502
                     /gene="mak"
                     /locus_tag="OO332_003009"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5943413.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2645..3826)
                     /gene="araJ"
                     /locus_tag="OO332_003010"
     CDS             complement(2645..3826)
                     /gene="araJ"
                     /locus_tag="OO332_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5943414.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(3952..7095)
                     /gene="sbcC"
                     /locus_tag="OO332_003011"
     CDS             complement(3952..7095)
                     /gene="sbcC"
                     /locus_tag="OO332_003011"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5943415.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7092..8294)
                     /gene="sbcD"
                     /locus_tag="OO332_003012"
     CDS             complement(7092..8294)
                     /gene="sbcD"
                     /locus_tag="OO332_003012"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5943416.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8484..9173
                     /gene="phoB"
                     /locus_tag="OO332_003013"
     CDS             8484..9173
                     /gene="phoB"
                     /locus_tag="OO332_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5943417.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9231..10526
                     /gene="phoR"
                     /locus_tag="OO332_003014"
     CDS             9231..10526
                     /gene="phoR"
                     /locus_tag="OO332_003014"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5943418.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10933..12252
                     /gene="brnQ"
                     /locus_tag="OO332_003015"
     CDS             10933..12252
                     /gene="brnQ"
                     /locus_tag="OO332_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5943419.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12328..13701
                     /gene="proY"
                     /locus_tag="OO332_003016"
     CDS             12328..13701
                     /gene="proY"
                     /locus_tag="OO332_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5943420.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            13857..>14636
                     /locus_tag="OO332_003017"
     CDS             13857..>14636
                     /locus_tag="OO332_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HCR5943421.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWF"
BASE COUNT         3227 a         3732 c         3944 g         3733 t
ORIGIN      
        1 gcgtcaccgg cttgaggaaa aacaatgaca caacgtccat ggagcaagtt acagcgggag
       61 atctatgact tactgacgcc aacgatcaac ctgcaaattc actgtacccg ttatcccatg
      121 cgcagccaaa atggtggaag taccgattta ccgcgttatt ggatcacgtt ggataaaaac
      181 gtgatctggg attacccgaa agactttatt gcaggtaacg ctggagtgcg taattttcac
      241 ggtgaaactt gctggtatcc ttacctgacg gatatttgtt caatttctga tttattgcgt
      301 gaatatattg ataccccaaa agcggagctg ttaacaaaac aatttaccag cgataaatgg
      361 gggttagtga atattctgcg tgctgctgac aggcgaatag ggatgaggcg attagatcag
      421 ttacggcgta aaacgcataa tattgctgcg ctaaaaatta ttgctcgccg tagcgaataa
      481 ttatatgcct ggtgtggctt cgtacgccgg ataagacgcg gcaggcgtcg catccggcat
      541 taaaggaaaa tcagcaatta acgttgtgct tcgccaccta atccttcaat caggttttga
      601 attaacgctg ccagttcacc agtcatcagg atgaaatcgg catcaaaacg ctgggcgaaa
      661 tcttcacggt cgatatcttc gttttgatcg tgcagctcgt cgcagaactt aagacgcttg
      721 agcgaaccat cgtcgcacat cacaaactga atgcgctgct gccagtcgag cgccagttta
      781 gtcaccactt ttccggcttc aatgtgattg gtgatctctt cgctggtcag atcttgtttc
      841 ttcgcgcgga tcacgccgcc atcttccagc aacgatttca gctcggcttc atcaagcagc
      901 tggaagccct gtgccgcact accggagcga acccattcgg tcagcgtcag ttcaatcggg
      961 ttttccatgc tcaatggtac aaccggtaac gaccccaggc ttttacgcag taatgccagc
     1021 gtatcttccg cttttttggc actggcgcag tccaccataa tcaaaccgtt aaccgtgtcg
     1081 agccacatca ttgtctggct aaaacggctg aaagcacgcg gcagcagaga gtgcagcact
     1141 tcgtctttca gcgaatcttt ttcggttttc ttcagcttac gcgcctgttc cgcttccagt
     1201 ttggcgattt tcgcttccag cgcctgttta atcaccggag acgggaggat tttttcttct
     1261 ttgcgcgcgc agataacaat ttgaccattg gcaacgtgcg ttaacgcatc gctgtgcgat
     1321 cccatcggag gaacccagcc catcttcgcc atgtcctggc tgccgcatgg ggtaaatgcc
     1381 atcgaggcta gctgtttttc catctcttct gcacgcagcg aaatctcgcg gctaagacgg
     1441 taaaccatta aatttttgaa ccacagcatg ataatttcca cggccttgtc gttaaattta
     1501 gcgggcatga taacgaattg tcggcggcct tgcattgcca atccggttgt ccgtctctac
     1561 gctattgata ttgaaaaaaa taaggagagt acagtgcgta taggtatcga tttaggcggc
     1621 accaaaactg aagtgattgc actgggcgat gcaggggagc agttgtaccg ccatcgtctg
     1681 cccacgccgc gtgatgatta ccggcagact attgaaacga tcgccacgtt ggttgatatg
     1741 gcggagcagg cgacagggca gcgcggaacg gtaggtatgg gcattccagg ttcgatttcg
     1801 ccctacaccg gtgtggtgaa gaatgccaat tcaacctggc tcaacggtca gccattcgat
     1861 aaagacttaa gcgcgaggtt gcagcgggaa gtgcggctgg caaatgatgc caactgtctg
     1921 gcggtttcag aagcagtaga tggcgcggca gcgggagcgc agacggtatt tgccgtgatt
     1981 atcggcacgg gatgcggcgc gggcgtggca ttcaatgggc ggacgcatat cggcggcaat
     2041 ggcacggcag gtgagtgggg acacaatccg ctaccgtgga tggacgaaga cgaactgcgt
     2101 tatcgcgagg aagtcccttg ttattgcggt aaacaaggtt gtattgaaac ctttatttcg
     2161 ggcacgggat tcgcgacgga ttatcgtcgt ttgagcggac atgcgctgaa aggcagtgaa
     2221 attatccgcc tggttgaaga aagcgatccg gtagcggaac tggcattgcg tcgctacgag
     2281 ctgcggctgg caaaatcgct ggcacatgtc gtgaatattc tcgatccgga tgtgattgtc
     2341 ctggggggcg ggatgagcaa tgtagaccgt ttatatcaaa cggttgggca gttgattaaa
     2401 caatttgtct tcggcggcga atgtgaaacg ccggtgagta aggcgaagca cggtgattcc
     2461 agcggcgtac gcggcgctgc gtggttatgg ccacaagagt aaaaaacgta ggcaattggc
     2521 gcatcatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaaaatg tgccagaacc
     2581 gcgtagggcg gataaggcgt tcacgccgca tccggcaata agcaacgcgc accgtgacag
     2641 taatctaccc cagtggtttc gccagcaccg gactatccgc cgcttgctgg cgcttatagc
     2701 gaccatacag cagcaacgat gacatcgcag caaacgaaaa cagggcggca ggcagtgcca
     2761 cgtaattata tgccagcccc agcgtcagca tcatacctcc gcaatatgcg ccgacggcgc
     2821 taccgaggtt aaacgctatt tgcccacctg cggcacctaa taactctccg cctttggcat
     2881 tttgcagtaa caatatttgc agaggtgctg aaagggcaaa taatcccgcg caacaaataa
     2941 aagcaaaaat aagcgacgtt gttttcatgc cgccgcagaa aaagagcatc agcagtgcca
     3001 gtacaatgat aaagtcagtc actgctgcaa tgcgcagtgg tgaataacgt cctgaaatcc
     3061 tgccacttag catatttccc agcaccatcc ctagcccaac taacatcata ataaaggtca
     3121 tcgccgtttc cgaaaaaccg gaaataaaca tcatgtatgg ctttacgtag ctgaaccagg
     3181 caaacacacc tgcgttgcca aacatcgtgg cggcgaaaat taaccacggg gccgggctgc
     3241 gcaaaaagtg aaattgttcg cgcagatttc ctttcgcctc gtcgcgaata tctggcaccc
     3301 aaaaatagac cgatgccatc accgcaatat taaaaacagc gatcaataaa aaggtgtaac
     3361 gccagcattc ctgacttaaa tacgttccca gcggaatgcc cagcaaattg gcgactgtca
     3421 tcccggaaac catccccgcc acggcggcgg tgacttttcc gggtttgata atttttgata
     3481 acacgatcgc tccgacgcca aaaaatgcgc catgcggaaa gccggatacc agccgaccaa
     3541 tggcgagcat caggtaagac gaagagagcg tgaacatggc gttgccaatg acgcacaacg
     3601 ccaccagaaa caacaagata tgtttgagtg agtagcggct ggaaaagagt gcgatgattg
     3661 gcgcaccgac caccaccacc agtgcataat acgagatcat atgcccggca gcaggaatcg
     3721 aaattcctac gttatgcgcc agctccgtga gcacgctcat aatgccaaat tcggctatcc
     3781 ccaaaccaaa cgtgcccaga gccaaagata aaatgacttt tttcatacca ccatcacaac
     3841 caccagaacg gctaccaatc accgtagcca ataaagtgat agcttgcagg ataacgacca
     3901 gcaaggccaa gagataattc gcccctctgt attcattatc ctgctgaata gttatttcac
     3961 tgcaaacgta ctttccagtt tgctgtagcc caggccgttg atctttttca ctttgatctg
     4021 caccggaata cgctctttca tcgcttctac gtggctaatc acaccgatgg ttttgccact
     4081 ggcgttcagg gcatccagcg catcaagggc ggtatccagc gtttcgctat ccagcgtgcc
     4141 aaaaccttca tcaaggaaca gcgagtcaat acgtgtttta tggctgacca gatccgaaag
     4201 cgccagcgcc agcgccagac taacgaggaa actttcgccg ccggaaaggg tacgggtatc
     4261 gcgtaccgca tctgcctgcc aggtatcaac aacctcgact tccagcgcct cgctggcttt
     4321 gcgctgtaac agatagcgcc cgtgcagccg ggtaagttgc tgattagcga gatggactaa
     4381 attatccagc gtcagcccct gggcaaactt gcggaattta tcgccctctt tggaacctat
     4441 tagcgaattc agatatcccc agtcctcaac ctgctgcgtc atttgagcaa tttgctgcat
     4501 taaggtttgt tgttgctgac ggttatctgc atcctgcttc agctgctggc gaatctcgcc
     4561 ttgactcgtg gtgttttcac gcaacttttg gtgagtttgc gctaactctt gctgaatctg
     4621 ctccaccgtc acagtgagag ccaacccgtc aggtcggtgt tgttgatgct gtgccagcgt
     4681 ttctgctgtc tgagtgacca gcgtttgcgc ctgacggcgc tggttttcca gattctgctt
     4741 gagctgttcc agctgcgtta gtgtttgttc atccattagc gccgcaagga acgcctgctg
     4801 atcgtcaaag acgctggcct gtagcgtggt gtcaaactgc gcttgggctt tttgcagact
     4861 ttgcgccgcc agaacatcct gttgctgtaa cgtctgctgc tggctgtgta atgcgagaca
     4921 ttgttcatga acctgccgcc agttatccag cgccacagtt tcttcgctgt gcgggagatc
     4981 atcactttgc ggcaacgttt ccagaatcgg cgtcagctgc tgaatacggt tttgcagcgc
     5041 ggttaattcg ttctggcgtt gctgccagct ctgcgcttct tgctgacgtg tcgccaacca
     5101 gctctcttct tcatcttcct gtggcaatgt cagtgcataa cccgccaatg ccgttaagag
     5161 ctgctgctga cgttgttcaa tttgctgttg atactggata atttgctgat tatgcgcggc
     5221 aatctgccct tgtaattcat gtcgttggct gagtaaccgc agctggcgtt cgtgctcatc
     5281 ttgtgcatcc agccacggtt gaatatcgtc ctgtggctgc aaggtgatat tgaggctggc
     5341 cgtgacggct tgccattgtt gagtaagtgc ttgctcatct tgtcggaggc tttgcgcttc
     5401 gttttcatcg cgctgaagct gcttcgttaa tgcatccagt tgcccgcgca gcgccgcacc
     5461 ttcttcgccg agctttttaa cttcgttttc cagcgccagt aatcgagact gattaacacc
     5521 aggctccagc gcctgatacg cctcgaccgc cgggtggctg gtggaaccac aaagtggaca
     5581 aggctgaccc gcctgtaact gtgcacgctg ggcttccagc gttttgatgc gcgcttcctg
     5641 ctcgcaaatg gttttcacat cggcaagttg ctgcgtcttt tctttataac gctggcgcat
     5701 ttcgttaagt gcgacattac gttgcgtctg ttccagagtg acattctgga tagtgaccat
     5761 taactgcgcc agacgttttt gttggggaac aatctgccca tgcagcgcga ccaggcgctg
     5821 acgcagtggg cgttgctcgg catgttgcgc cagggcggta gcaacttcat cggcggttaa
     5881 cgtcaacgtg atcgccgcaa gcgcattaag tttttgctca gcatgggtta actgttgctg
     5941 ccattgccgc agatgctcgc gatcgctggt ttgttgggag aactgcgcac gccaacccgc
     6001 cagttcgttg ttccactgac ggaagcggtc gtgttcctgt aaccaggtat tcaggctttg
     6061 ttgctgctgc tgtaattctg ctgactgctt cgccgcgtgg tggcgaatgc tcgcgcgaag
     6121 cgccattgtg ctctgtaagc gagtatttac ttcttcaatc tgctggcgaa tatgcgccag
     6181 cgccgcgctg tgttctgcga tgcgttccca gtgtggacga agatttcgtg ccggttgtgc
     6241 cagactaagc gccgccagtt gaggttgcgc tttttcttct tcggctaacg cctgttgcaa
     6301 ggcctgctga cggcggctgg cttcttgctg caattcgtcc tgacgcgtta accagtttag
     6361 cgattgttgt tcttgctgct gcgcggtaat taactgtttt tcttcgtcag taagtacctg
     6421 caaactcgct gtcagcgatt gcacttgttc cggcgtgagc aacgtgacgc cgctggcctg
     6481 cgcttgcagc ttctccagct cagtacgggc cgatttgtgc tgctcaaaaa ccatcgccga
     6541 gatttgcccg tagatttcag tgccggttaa ctcctcgagc aattccgcgc gttctttggg
     6601 tttggcattc aggaaggcag caaattgccc ctgcgaaagc agcatcgaac gggtgaagcg
     6661 cccgtaatcc agcccggtta acgtcgctgt cagttccagc ttatctttca ctttgtcggc
     6721 gagaattttg ccgtcggcgc agcgcgccag ctctacgcga ggcacctgca aattaccgtc
     6781 cggctggtta cgcgcccgat tctggctcca gaacgcgcga tacgcttcac ctttcacttc
     6841 aaactccacc tccgccagac attcagcggt atcgcgggtc atgagatcat tttgcgattg
     6901 tgaaacgtta gagagacgcg gagtttcgtg atacagcgcc agacaaatgg cgtccagcag
     6961 ggtggttttc cccgcaccgg ttgggccggt aatggcaaac agcccgttgc tggcgaacgg
     7021 ctcgcgggtg aaatcaatct tccattcgcc ttttaatgag ttcaggtttt tcaggcgcag
     7081 gctgagaatt ttcatgcttc gtgttctccg gcgagggtat gcaacgtcgt ggtgaaaaga
     7141 tgctgcagac gttgctgctg cgattcatcc agttcttcca gtgccagacg gcgattgaac
     7201 acctcttcga cgctgagttc gctgagggtt tcacgctgtt ggctggctaa cacacgctcg
     7261 cgctgttcac gactccgacg taccagcaat acttcgacag gcaatgattc ggttaatgcc
     7321 tggattttgc gctgaatatc atgcagatac tcatcagtag tgatttcgat atccagccag
     7381 acaggtggct cctgcgatac atcgcgccac tgttccagct gtgcggtaat cgacgccaga
     7441 tcgcctttca gcaccgccat gggttgagtt accggtacgt tcaggttttc cacgctctct
     7501 aatttgccgt ttgaaaatgt caccagatgg acatatttac tcttaccgca ttcatcaaaa
     7561 ctcagtggaa tgggggaacc gcaatagcga acatgttcca tgccgccaat aatctgtgcg
     7621 cggtgaatat gcccgagcgc gatgtagtcg gctggtggaa agttttgtgc cggaaacgcg
     7681 tccagcgtgc caatataaat gtcacgcacg gcgtcacttt tactggcacc cacggtcgtt
     7741 aaatgtcccg tggcgatgat gggcagaggc tgatcgccgc gcagtttgca ggcatcggca
     7801 tagtgttgtt ggtaataatc ggtaatcgct gccagtaaat gctgctgttt ttcaataccg
     7861 ttaagccccg cctggctgga aataatgtca cgcgggcgta aaaacggaat ggggcacagc
     7921 actgcgcctg gcgtcccgtc gcgacgaggc aagatttgcg gcgcatgtcc ggcgctggcg
     7981 accacggttg tattgaggaa cgccatgata tcgcgcgatt cattcagcgt ggcgaccgag
     8041 tcatggtttc ctgccagtac caccagatga cagccagttt gctgtaaatt gacaacaaaa
     8101 cggttgtata acgtgcgggc gtaactgggc ggcgagccgg tatcgaaaac atcaccagca
     8161 acaataatcg catccacctg atgggtttgt gctgtctcca gcagccagtc aagaaaagcc
     8221 tgatgttcag cttcgcggct tttactgtag aagttctggc cgagatgcca gtctgaggtg
     8281 tgaaggatgc gcataacggt tccctggcga aaaagcatgg tcgcgattat acccaaacag
     8341 atgtgccatt tgcttttttc tgcgccacgg aaatcaataa cctgaagata tgtgcgacga
     8401 gcttttcata aatctgtcat aaatctgacg cataatgacg tcgcattaat gatcgcaacc
     8461 tatttattac aacagggcaa atcatggcga gacgtattct ggtcgtagaa gatgaagctc
     8521 caattcgcga aatggtctgc ttcgtgctcg aacaaaatgg ctttcagccg gtcgaagcgg
     8581 aagattatga cagtgctgtg aatcaactga atgaaccctg gccggattta attctccttg
     8641 actggatgtt acttggcggc tccggtatcc agttcatcaa acacctcaag cgcgagtcga
     8701 tgacccggga tattccagtg gtgatgttga ccgccagagg ggaagaagaa gatcgcgtgc
     8761 gcggccttga aaccggcgcg gatgactata tcaccaagcc attttcgccg aaggagctgg
     8821 tggcgcgaat caaagcggta atgcgccgta tttcgccaat ggcggtggaa gaggtgattg
     8881 agatgcaggg attaagtctc gacccgacat ctcaccgagt gatggcgggc gaagagccgc
     8941 tggagatggg gccgacagaa tttaaactgc tgcacttttt tatgacgcat cctgagcgcg
     9001 tgtacagccg cgagcagctg ttaaaccacg tctggggaac taacgtgtat gtggaagacc
     9061 gcacggtcga tgtccacatt cgtcgcctgc gtaaagcact ggagcccggc gggcatgacc
     9121 gcatggtgca gaccgtgcgc ggtacaggat atcgtttttc aacccgcttt taacgccttg
     9181 ctcatcggac gcagagcagg gcttatgatt tcttaactgg agtatcttac gtgctggaac
     9241 ggctgtcgtg gaaaaggctg gtgctggagc tgctactttg ctgcctcccg gctttcatcc
     9301 tgggtgcatt ttttggttac ctgccctggt ttttgctggc atcggtaaca ggactgctta
     9361 tctggcattt ctggaattta ttgcgccttt catggtggct gtgggtggat cgcagtatga
     9421 ccccgccacc ggggcgtggt agctgggaac cgctactata cggcttacac cagatgcagc
     9481 tgcgaaataa aaaacgccgc cgtgaactgg gcaatctgat taaacgcttt cgtagcggcg
     9541 cggagtcgct gcccgacgcg gtggtgctga ccacggaaga gggcggtatt ttctggtgta
     9601 acggtctggc gcaacaaatt cttggtttgc gctggccgga agataacggg cagaacatcc
     9661 ttaacctact gcgttacccg gagtttacgc aatatctgaa aacgcgtgat ttttctcgcc
     9721 cgctcaatct ggtgctcaac accgggcggc atctggaaat tcgcgtcatg ccttataccc
     9781 acaaacagtt gttgatggtg gcgcgtgatg tcacgcaaat gcatcaactg gaaggggcgc
     9841 ggcgtaactt ttttgccaac gtgagccatg agttacgtac gccattgacc gtgttacagg
     9901 gttacctgga gatgatggat gagcagccgc tggaaggcgc ggtacgcgaa aaagcgttgc
     9961 acaccatgcg cgagcagacc cagcggatgg aagggctggt gaagcagttg ctaacgctgt
    10021 cgaaaattga agcctcgccg acgcaattgc tcaatgaaaa ggttgatgtg ccgatgatgc
    10081 tgcgcgttgt tgagcgcgag gctcagactc tgagtcagaa aaaacagaca tttacctttg
    10141 agatagataa cggcctcaag gtgtctggca acgaagatca gctacgcagt gcgatttcga
    10201 acctggtcta taacgccgtg aatcatacgc cggaaggcac gcatatcacc gtacgctggc
    10261 agcgagtgcc gcacggtgcc gaatttagcg ttgaagataa cggaccgggc attgcaccgg
    10321 agcatattcc gcgcctgacc gagcgttttt atcgcgttga taaagcgcgt tcccggcaaa
    10381 ccggcggtag cggattaggg ttagcgatcg tgaaacatgc ggtgaatcat cacgaaagtc
    10441 gcctgaatat tgagagtaca gtaggaaaag gaacacgttt cagttttgtt atcccggaac
    10501 gtttaattgc caaaaacagc gattaatccg cctttgtcat cttttattgc cataagccag
    10561 ccgatgctgg cttattttct ttgcagtcaa aatacgggcg ttagatttta caacggttgg
    10621 tgattttttg ttcgcatgat tagccatgtc tttttcacgg aaatagtgtt ttatactggt
    10681 tggtgatttc ttatcgctat atacctctgg tttttagatc cctccttgct ttaaaacgtt
    10741 ataagcgttt aaattgcgct tcaggtgctg tcatactgac tgcattaacg cggtaaatcg
    10801 aaaaactatt cttcgccgcg cctggttggg agtatttccc gctaaaattg tttaaatata
    10861 ccgctgtatc atccccaggg attggcacaa aaatttaacg ttacaacacc acatccacag
    10921 gcagtatgat ttatgaccca tcaattaaga tcgcgcgata tcatcgctct gggctttatg
    10981 acatttgcgt tgttcgtcgg cgcaggtaac attattttcc ctccaatggt cggcttacag
    11041 gcaggcgaac acgtctggac tgcggcattc ggcttcctca ttactgccgt tggcctgccg
    11101 gtattaacgg tagtggcgct ggcaaaagtt ggcggcggtg ttgacagcct cagcacgcca
    11161 atcggtaaag tcgctggcgt actgctggca acggtttgtt acctggcggt ggggccgctt
    11221 ttcgctacgc cgcgtacagc taccgtttcc tttgaagtgg ggattgcgcc gctgacgggt
    11281 gattccgcgc tgccgctgtt tatctacagc ctggtctatt tcgctatcgt tattctggtt
    11341 tcgctctatc cgggcaagct gctggatacc gtgggcaact tccttgcgcc gctgaaaatt
    11401 atcgcgctgg tcatcctgtc tgttgtcgcg attgtctggc cggcgggttc tatcagcacg
    11461 gcgactgagg cttatcaaaa cgctgcattt tctaacggct tcgttaacgg ctatctgacc
    11521 atggatacgc tgggcgcaat ggtgtttggt atcgttattg ttaacgcggc gcgttctcgt
    11581 ggcgttaccg aagcgcgtct gctgacccgt tataccgtct gggctggcct gatggcgggt
    11641 gttggtctga ctttgctgta cctggcgctg ttctgtctgg gttcagacag cgcgtcgctg
    11701 gtcgatcagt ctgcaaacgg cgctgctatt ctgcatgctt acgttcagca cacctttggc
    11761 ggcggcggta gcttcctgct ggcggcgtta atcttcatcg cctgtctggt cacggcggtt
    11821 ggcctgacct gtgcttgtgc agaattcttt gcccagtacg taccgctctc ttatcgtacg
    11881 ctggtgttta tcctcggcgg cttctcgatg gtggtgtcta acctcggctt aagccagctg
    11941 atccagatct ccgtaccggt gctgaccgct atttatccgc cgtgtatcgc actggttgta
    12001 ttaagtttta cacgctcatg gtggcataat tcgtcccgcg tgattgctcc gccgatgttt
    12061 atcagcctgc tttttggtat tctcgacggg atcaaagcat ctgcattcag cgatatctta
    12121 ccgtcctggg cgcagcgttt accgctggcc gaacaaggtc tggcgtggtt aatgccaaca
    12181 gtggtgatgg tggttctggc cattatctgg gatcgcgcgg caggtcgtca ggtgacctcc
    12241 agcgctcact aaatcactga acatttgttt taaccacggg gctgcgatgc cccgtggttt
    12301 tttattgtgt tgatgggtta ggaattgatg gaaagtaaga ataagctaaa gcgtgggcta
    12361 agtacccgcc acatacgctt tatggcactg ggttcagcaa ttggcaccgg gctgttttac
    12421 ggttcggcag acgccatcaa aatggccggt ccgagcgtgt tgttggccta tattatcggt
    12481 ggtatcgcgg cgtatatcat tatgcgtgcg ctgggggaaa tgtcggtaca taacccggcc
    12541 gccagctctt tctcgcgtta tgcgcaggaa aaccttggcc cgctggcagg ttacattacc
    12601 ggatggacct actgctttga aatccttatt gtcgccatcg ccgatgtgac cgcttttggt
    12661 atctatatgg gtgtcttgtt cccgacggtg ccgcactgga tttgggtact gagcgtggtg
    12721 ctgatcattt gcgccgtaaa cctgatgagc gtgaaggtct tcggtgagct ggaattctgg
    12781 ttctcgttct ttaaagtcgc caccatcatc atcatgattg tcgccggttt cggcatcatc
    12841 atctggggga ttggcaacgg cgggcaaccg accggtattc ataacctgtg gagcaacggc
    12901 ggcttcttca gtaacggctg gcttggcatg gtaatgtcgt tgcaaatggt gatgtttgct
    12961 tacggtggga tcgaaattat cgggattacc gccggtgaag cgaaagatcc tgagaaatcg
    13021 attccgcgtg cgattaactc cgtgccgatg cgtattctgg tgttctacgt cggtacgctg
    13081 ttcgtcatta tgtctatcta cccgtggaat caggttggca ctgccggtag cccgttcgtg
    13141 ctgacgttcc agcatatggg cattaccttt gccgccagca ttcttaactt tgttgtgctg
    13201 accgcttcgc tgtcggcaat taacagtgac gtatttggcg taggccgtat gctccacggt
    13261 atggcagagc agggcagcgc accgaaaatt ttcagcaaaa cgtcgcgtcg cggtattccg
    13321 tgggttacgg tgctggtgat gactaccgcg ctgctgtttg cggtgtatct gaactacatc
    13381 atgccggaaa acgtcttcct ggtgatcgct tcgctggcaa ccttcgccac ggtatgggtg
    13441 tggattatga tcctgctgtc gcaaatcgcc ttccgtcgcc gtttgccgcc agaagaagtt
    13501 aaggcgctga aatttaaagt gccgggtggg gtagcaacga ccatcggcgg gctgattttc
    13561 ctgctcttta ttatcgggtt gattggttat cacccggata cgcgtatctc gctgtatgtc
    13621 ggtttcgcgt ggattgttgt gctgttgatt ggctggatgt ttaaacgccg ccacgatcgt
    13681 cagctggctg aaaaccaata atacttgcgt tctgtaagcc ggttcaggag gaaatcctga
    13741 tccggctatt ccgaaagtta tccgcccccg tcctcctccc ccaaatatcc ttcagatgat
    13801 gagtgatcct gcattaggct atggcaaggt gatcagattt tcatcacagg ggaattatga
    13861 tgttaaatgc atggcacctg ccggtgcccc catttgttaa acaaagcaaa gatcaactgc
    13921 tcattacact gtggctgacg ggcgaagacc caccgcagcg cattatgctg cgtacagaac
    13981 acgataacga agaaatgtca gtaccaatgc ataagcagcg cagtcagccg cagccaggcg
    14041 tcaccgcatg gcgtgcggcg attgatctct ccagcggaca accccggcgg cgttacagtt
    14101 tcaaactgct gtggcacgat cgccagcgtt ggtttacacc gcagggcttc agccgaatgc
    14161 cgccggcacg actggagcag tttgccgtcg atgtaccgga tatcggccca caatgggctg
    14221 cggatcagat tttttatcag atcttccctg atcgttttgc gcgtagtctt cctcgtgaag
    14281 ctgaacagga tcatgtctat taccatcatg cagccggaca agagatcatc ttgcgtgact
    14341 gggatgaacc ggtcacggcg caggcgggcg gatcaacgtt ctatggcggc gatctggacg
    14401 ggataagcga aaaactgccg tatctgaaaa agcttggcgt gacagcgctg tatctcaatc
    14461 cggtgtttaa agctcccagc gtacataaat acgataccga ggattatcgc catgtcgatc
    14521 cgcagtttgg cggtgatggg gcgttgctgc gtttgcgaca caatacgcag cagctgggaa
    14581 tgcggctggt gctggacggc gtgtttaacc acagtggcga ttcccatgcc tggttt
//
LOCUS       DAJTRQ010000100        14526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000100 DAJTRQ010000000
VERSION     DAJTRQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1037
                     /locus_tag="OO332_003018"
     CDS             <1..1037
                     /locus_tag="OO332_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5943422.1"
                     /translation="YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINL
                     IDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQA
                     LDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLD
                     ALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLA
                     EVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYV
                     DIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEA
                     VHQVYTYPAP"
     gene            complement(1070..2263)
                     /gene="lplT"
                     /locus_tag="OO332_003019"
     CDS             complement(1070..2263)
                     /gene="lplT"
                     /locus_tag="OO332_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5943423.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2256..4415)
                     /gene="aas"
                     /locus_tag="OO332_003020"
     CDS             complement(2256..4415)
                     /gene="aas"
                     /locus_tag="OO332_003020"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5943424.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4804..5835
                     /gene="galR"
                     /locus_tag="OO332_003021"
     CDS             4804..5835
                     /gene="galR"
                     /locus_tag="OO332_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5943425.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(5842..7104)
                     /gene="lysA"
                     /locus_tag="OO332_003022"
     CDS             complement(5842..7104)
                     /gene="lysA"
                     /locus_tag="OO332_003022"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5943426.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7226..8161
                     /gene="lysR"
                     /locus_tag="OO332_003023"
     CDS             7226..8161
                     /gene="lysR"
                     /locus_tag="OO332_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5943427.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8148..8840)
                     /gene="ygeA"
                     /locus_tag="OO332_003024"
     CDS             complement(8148..8840)
                     /gene="ygeA"
                     /locus_tag="OO332_003024"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5943428.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(8969..10357)
                     /gene="araE"
                     /locus_tag="OO332_003025"
                     /pseudo
     CDS             complement(8969..10357)
                     /gene="araE"
                     /locus_tag="OO332_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(10672..11433)
                     /gene="kduD"
                     /locus_tag="OO332_003026"
     CDS             complement(10672..11433)
                     /gene="kduD"
                     /locus_tag="OO332_003026"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5943429.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11463..12299)
                     /gene="kduI"
                     /locus_tag="OO332_003027"
     CDS             complement(11463..12299)
                     /gene="kduI"
                     /locus_tag="OO332_003027"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5943430.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12619..13767)
                     /locus_tag="OO332_003028"
     CDS             complement(12619..13767)
                     /locus_tag="OO332_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5943431.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14022..14526
                     /locus_tag="OO332_003029"
                     /pseudo
     CDS             14022..14526
                     /locus_tag="OO332_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         3761 a         3944 c         3446 g         3375 t
ORIGIN      
        1 aatatcaccg tataccccac agttcgctgg aagtcagcac gctggggctt ggcacgatga
       61 cgtttggtga acagaacagc gaagccgacg cccacgcaca actcgactat gccgtcgctc
      121 agggcattaa ccttatcgac gttgccgaaa tgtacccagt acctccgcgc cccgaaacgc
      181 aagggttaac cgaaacctac gtcggcaact ggctggcgaa acatggcagc cgcgaaaagt
      241 taattatcgc ctccaaagtg agcggaccgt cgcgcaataa tgacaagggc atccgcccgg
      301 atcaggcgct ggatcggaag aatatccgcg aagcgctgca tgacagcctc aagcgcctac
      361 agactgatta cctcgatctt tatcaggtgc actggccgca gcgcccgacc aactgcttcg
      421 gcaaactcgg ttatagctgg acggattctg cgcctgcggt ttcgctgctg gatacgctgg
      481 acgcactggc agagtaccaa cgcgcgggaa aaattcgtta tatcggcgtg tcgaacgaaa
      541 ctgcatttgg cgtaatgcgc tacctgcatc tggcggacaa acacgatctg ccgcgtattg
      601 tcaccattca gaacccttac agtctgttaa accgcagttt tgaagtaggt ctggcagaag
      661 tcagccagta tgaaggggtc gaactgctgg cctattcgtg cctgggtttc ggcacgctga
      721 ccgggaaata tctcaatggt gcaaaacccg ctggcgcacg taatacgctc tttagtcggt
      781 tcacccgcta tagcggtgag caaacgcaaa aagccgtcgc ggcgtatgtt gatatcgcca
      841 gacgtcatgg ccttgatcct gctcagatgg cgctcgcgtt tgttcgccgt caaccgtttg
      901 ttgccagcac tctgctgggc gcaaccacga tggatcagct gaaaactaac atcgaaagtt
      961 tgcatctgga gttaagcgaa gacgtattag ctgaaattga agcggtacat caggtttata
     1021 cttatcctgc accataaaga ttaacgccgg ttaaaaccgg cgttaaatat taatgacggc
     1081 gctgccagat ccacagcgcc gttattgcca gcgcaaatag cgcaccgaag ccaatgccaa
     1141 tgggcacgac cgggatgcct accattaccg ccagcgagta aatgcccagc atcaacaaca
     1201 tggcgctgtt ttcgccaagg ttttgtactg caatcgcatt ccccgccccg acgctttttt
     1261 taccccgctc ctgtagcaac gcattgagcg gaacgacaaa aaagcccccc agcacgccaa
     1321 tcagcatcaa caaggcatag gctggcagca gctcgtgttg cagggagaaa atcagtacca
     1381 ccacgccaat caaaatcccg gctggcatac agcgtgacac ggtttccagc gtaaccagct
     1441 tcgctgccgc acctgcgcca accacgatac caatcgctac catcgcgttg agataggtcg
     1501 gcgtagcgtt atcggtaatg cccagcgcca ccggtaccca cagcaccaac aggaaacgca
     1561 gcgtgacacc cgctccccag aataaactgg tgcccaccag cgaaaaacgc gtttcaccat
     1621 tgcaccatag cgaggtgcag gcattcagga aactgcgggt catgttgatg agattccagg
     1681 actgccccgg acgcgccgcc gccagtttgg gaatgtagat attggcaacg accgcaccac
     1741 cgtaggccag tgcgcatgcg gccagggcga ccaggacatg ccagtcagcc agcacaccac
     1801 cggctacgga accgagtaaa atcgccgcta tggcagaagc ttccattaaa ccgttagctt
     1861 ttactaactt actacccgtg gttaattcgc cgagaatacc gtatttcgcc ggtgaatagg
     1921 ctgcagcacc aacacccacc agcgtatagc cgagaaacgg attgatacca aagcagatac
     1981 tggctgcgcc cagcagcttc aggccgttgg caaacatcat cacccggcct ttggcgaagc
     2041 tatccgccac ctgcccgaca aacggcgcca aaagaatgta agcacctaca aacaccattt
     2101 gcaggatggg ctggctccac tccggataga actgcgcttt cagtaacgcc agagtggcaa
     2161 acagtagcgc gttatcgcca aacgcagaga gaaactgcgc cacgataacc gctttcatcc
     2221 ccttcgacca caacgaagtg ttagtgtgca ctgactcact catcgtgttg ttccgcttcg
     2281 tctacccagc ttttcaacgt gacaaagtca ggtttgccgc tgccaagtaa tggcatctgt
     2341 ttcagatagc gaatatcgcg cggtacagca agctccggca cgccgtgctc gcgggcatac
     2401 tgttgcaact tatcgcgcgt cagttcgtta tctgtggtga aaagcaccag tgcctcgcct
     2461 ttgctggcat cgctcttaat cgcagtggca tggactttat ctggcgaaac accaagtgcc
     2521 agttgttcca ccatttccag cgacaccatt tcgcctgcaa ttttggcaaa gcgttttgcg
     2581 cggccctgaa tctgcacaaa gccctgctcg tcaaaacgca caatatcgcc agtgtcatac
     2641 cagccgcgct ccatttcgcc gcgaacattc tcggcggtgg gcacttccag tacacctggc
     2701 ttctccaccc gcagatagcc gttcattatg ttcggccctt tcagttgcag gcgtccgccc
     2761 tcttcgatac cagggaccga caacaggcgt gcatccattc ctggtagaat acgccctacc
     2821 gtaccgggtt tcgccgccat cggtacgttg atagaaacga caggcgcgca ttcggtcaca
     2881 ccgtagcctt caaggatgcg caggccaaat ttatcctgcc aaagctgttt ggtactttct
     2941 tgtagttttt ctgcgcctgc caccacatag cgtagacgat agaagtcata cgggttggcg
     3001 aaacgcgcgt agtgaccgag gaaagtcgag gtaccgaaca acacggtgca actgcggtca
     3061 tacaccaagt ccggcacaat gcggtaatgc agcgggcttg gataaaggaa cacttctgca
     3121 cctgtaagca gtggcgtaaa caggcctacc gtcagcccaa aggagtgaaa cagcggtaat
     3181 gccgacataa agcgatcgtt ggtggtgaag tcggcaatcg ttttaatctg ctcgacgttc
     3241 gccagaatgc ttttatggct atggacgacg ccttttggat ggccttcaga accggaggta
     3301 aagaggatca gcgcctcttc ttccggctgc tgttttacct gcgccaggcg cggcatcagc
     3361 aaatgagcga agatccatac tttgtcggca gtggtgacat ctgcttttaa atcttccaga
     3421 tagacccagc gcacctgagt aagttgctcc ggcagatgcc agagtttgcc tttatcgaga
     3481 aactggcggg aagtgaagat ggttttgatt tcagccgccg taatagcact ggtcagccct
     3541 tttaccccgg cagtgtagtt catcattgcg ggaatgcggc gacgcgcgat ggccccaaaa
     3601 atcactgccg cactgatgcc tgcattgggc agcattaagc cgatgcgttc gccttcaaca
     3661 ctgtattttt caaggatgcg tccaacaaac agcgttttcg taagcaattt gcgataggag
     3721 tctggggtaa agttgacgtc ttcgacacat ttcttcccgg ctccgaagcg gtacattgca
     3781 ctcagtaaag attcgtacag agtttcacgc gggcgcaccg ccatacgcgc ttccatcatt
     3841 atttgatgca gcatttcgcc agcgattttg cgacggtcac gggcacgcgg cgcatccggc
     3901 atcgccacct gcgttggagg caaaatatgc agagtaattt gcgggaacaa gcgacgttta
     3961 accagacctt tcaggcggct gaagtgcgta agttccgccc cttcaatacg cacaggaata
     4021 accgttgcac cagacttcgc cgcgacaaaa cccgcgccat cgtagatttt catcagcgag
     4081 cctgtagtgg tgatgcgtcc ttcagggaaa atcaccactg gtcggccctg ttccaccaga
     4141 cgtaccagat gtttaatagc cataggttgc gtcgggtcga gaggaacaaa gtcgataaat
     4201 gatttcagcc aacgcatata ccactgttgg cttattgagg tgtaaacggc aaacactgga
     4261 cgcacaggta aaaacagtcc aagcaaaatg ccatcaataa aagagacgtg attaggcgta
     4321 attagaacgc gctcgccctt cagtgcctgg gtgtcacccg taacgcgaac gcgatacaaa
     4381 acacggcaca aatttcgaaa aaagctaaaa agcattccaa ctccctttgc tctgattcag
     4441 taaaagcgaa tggagggaga ttacacgaga taaagaacgc gagcgacagt aaattaggtg
     4501 cgaaaaaaaa cctgcgcatc cgcgcaggtt ggtgcaagag acagggtacg aagagcgtac
     4561 cgaataatct caccaatcaa tacctctggg atcaccactt tagcaacctg aagccaaacg
     4621 ccaccagcgg tcaatcgcaa cagcgtttcg caaagtgtaa ccaaaggttt gcaatctctt
     4681 tttttgtctt acttatttgc gtttggctca cattcccacg atgaaaacac gccacccctt
     4741 gaaccaacgg gcgttttccg taacactgaa acaatgtaag cgtttaccca ctaaggtatt
     4801 ttcatggcga ccataaagga tgtagcccga ctggcaggcg tttcagtcgc caccgtttcc
     4861 cgcgtcatta ataattcacc caaagccagc gaagcttccc ggctggctgt gcatagtgca
     4921 atggagtctc ttagctatca cccgaacgcc aacgcccgtg cgctggcgca gcagaccact
     4981 gaaacggtcg gtctgatcgt tggtgatgtt tccgatccgt ttttcggtgc aatggtgaaa
     5041 gcggtcgaac aggtggctta tcacaccggt aatttcttat tgattggcaa cggttaccac
     5101 aacgaacaaa aagagcgtca ggctattgag caactgatcc gccatcgctg tgctgcgttg
     5161 gtcgtccatg ccaaaatgat cccggatgcc gatttagcct cattaatgaa acaaatgccc
     5221 ggtatggtgc tgatcaaccg tatcctgcct ggctttgaaa accgttgtat tgctcttgac
     5281 gatcgttacg gtgcctggct ggcaacgcgt catttaattc agcaaggtca tacccgcatt
     5341 ggttatctgt gctctaacca ctctatttct gacgccgaag atcgtctgca agggtattac
     5401 gatgcccttg ctgaaagtgg tattccggcc aatgatcggc tggtgacatt tggcgaacca
     5461 gacgaaaacg gcggcgaaca ggcaatgacc gagcttttgg gacgaggaag aaatttcact
     5521 gcggtagcct gttataacga ttcaatggcg gcgggcgcga tgggcgttct caatgataat
     5581 ggtattgatg taccgggtga gatttcgtta attggctttg atgatgtgct ggtgtcacgc
     5641 tatgtgcgtc cgcgcctgac caccgtgcgt tacccaatcg taacgatggc gacccaggct
     5701 gccgaactgg ctttggcact ggcggataat cgccctctcc cggaaattac taatgtattt
     5761 agtccgacgc tggtacgtcg tcattcagtg tcaactccgt cgctggaggc aagtcatcat
     5821 gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc agtaattctt cgatggtctg
     5881 gcgacggcga atcaaccgcg cctgaccatt atcaaacaga acttctggta acagcggacg
     5941 gctattgtag ttggatgaca ttgatgcgcc atatgcccct gtatcatgca gtaccagata
     6001 atcacctgcc ttcacttccg gcaaggcgcg ggtttcaaca tttccccctt cctgctgggt
     6061 aaagacatcg cccgattcac ataacggccc ggcgacgacg gtttccaccg ttggcgcgtg
     6121 ttccagagaa cgaccatcag ctgccagggc actgatatgg tggtaactac cgtacattgc
     6181 cgggcgcatc agatcgttga acccggcatc aaccagcaca aagtggcggc tacccatttg
     6241 tttgacgctc cgcacctgag taattaatac gccagactgc gctaccagga agcgacccgg
     6301 ttcaatttcc agtttcacag ggtggcccaa atggcgagcg atttgctcac gcgcggcatt
     6361 ccacagacca taataatgtt cggtatcaac cgcctcttca ccctgttgat aaggaataga
     6421 aagcccaccg cccgcagaaa tagcctgtaa atcctgaccg aattcgatga cctgacgcac
     6481 catagcacca cacacctgct ccagatgggc ataatcaacg ccagaaccaa tgtgcatgtg
     6541 aatgccgact agctgcagat gatgacgttg tatcacgtcc agtgcggcgg gcagatcggt
     6601 gtaccagata ccgtgcttgc tgttttcgcc accggtattg gttttttggc tatgtccgtg
     6661 accaaacccc ggattaacgc gcagccatac ccgatgccct ggcgaaacct ggcccagttg
     6721 gtcgagcata tcaacagaac ccgcattcac cggaatttgc aattcactga cgcgttcaag
     6781 cgtcgcctga tcgataacat ctgccgtaaa aacaatatca tcggggtgcg tttgcggatt
     6841 gtaacccgcc gccaacgcac gctctatttc gcctaacgag acggaatcca ctttcacgcc
     6901 ctgctcacgc attaagcgca aaatatgaat attggaacag gctttctgtg caaagcgcac
     6961 cacttcaaac tgtttcagcg ctgctatctg ccgacgaata atttgcgcat cgtagaccca
     7021 caccgggcag ccaaattcag cgggcaaacg cagcagattt tcggcggtga gatcggtatc
     7081 ggtgctgaac agtgaatgtg gcataacaaa ctccagatga gtgctttttt atgattacgc
     7141 cacatcataa aaagaataaa aaatatcgat ttatgtcgaa tctatgcaaa aatgatatgg
     7201 attaccggat tgcgagagag cgctaatggc cgccgttaac ttacgtcata ttgaaatttt
     7261 tcatgcggta atgaccgccg gaagcctgac tgaggcagca cacctgctac acacctcaca
     7321 gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg atcgggctga aattgtttga
     7381 gcgcgtacgt ggacgattac atcctaccgt gcaaggactg cgtctgtttg aagaagtgca
     7441 acgatcctgg tacggactgg atcgcattgt cagcgccgca gaaagtctgc gcgagtttcg
     7501 ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg caatcttttt taccgcagct
     7561 cctgcaaccc tttctggcac gttatcccga tgtcagctta aatatcgtgc cccaggaatc
     7621 accgctactt gaagagtggc tctcggccca gcgtcatgat ttaggactca ctgagacact
     7681 ccatacgcct gcgggaacag aacgtaccga attactctct ttagatgaag tgtgtgtgtt
     7741 acctccgggt catccgctgg cggtaaaaaa ggtattaacg ccggatgatt ttcagggtga
     7801 gaattacatc agcctttccc gtactgacag ctatcgccag ttgctggatc agctatttac
     7861 tgaacatcag gttaaacgac gcatgatcgt agaaacccac agcgccgcgt cagtctgcgc
     7921 aatggtacgg gcgggggtag gtgtttcggt ggttaacccg ctcaccgcac tagattatgc
     7981 ggcaagcggt ttagtggtgc ggcggttcag tattgcggtt ccgttcaccg tcagcctgat
     8041 ccgccccctg caccgcccgt catcagcgct ggttcaggcg tttagtgggc atttacaagc
     8101 ggggttaccg aaactggtca cttctcttga cgctattttg tcgtcagcta cgacagcata
     8161 aaagcgacag catcctcggc atggatcgac gcggtatcaa acacaggcaa aacactgcgc
     8221 tcttctggca ccagtaaacc aatttctgtg cagccaaaaa tgacgccctg tgcgccctgt
     8281 tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg acgcttcggt aaatttcccc
     8341 agacacagtt cttcaaaaat aatctgatta attttcgccc gttcatccgc ttcaggaata
     8401 agacaattga tggaaaattg ttccgtcagc cgcccgcgat aaaaatcctg ttccatggtg
     8461 taacgcgtac ccagcagcgc cacacgagtc attccggccc cggtaattgc acgtccggtg
     8521 gcatccgcaa tgtgtaaaaa aggcagagtg caacgtgact caatggcatc cgccacttta
     8581 tgcatcgtat tggtacataa cacaatacct tctgcgcccg cccgctgtaa gccaagcgcc
     8641 gcctcagcca gaatgtcccc ggttttatcc cattccccgc gacgctggca ctcttctatt
     8701 tcatgaaaat cgacgctatg tagcagcatt tgcgcagagt gaagcccacc aagccgctgt
     8761 ttaatgcctt catttatcag acgatagtaa ggaatggtgg attcccagct cattcctccc
     8821 agcaaaccaa ttgttttcat tcctctctcc tgatgtgtgt taccgcaaca aggctgaata
     8881 aggaacgaga caaacgcctc aacggccagg tgcccaatct ctattaacga aaaaagggcc
     8941 ggatgtacag cacatccggc ccgtgaaatc agacgccgat atttctcaac ttctcgcctg
     9001 ccatcagttt gcgttcgata tgttccagcg tgacattttt ggtttccgga atgagccaga
     9061 aagtaatgcc cacaaacgca atgttcaacg cagtgtagag ccagaacgta ccggcagcgc
     9121 caatgctatc aagcagtgtc aggaaggccg cgccgataat catattcgac acccagtttg
     9181 tcgtcgtcga acaggtaata ccgaaatcgc ggcatttcag cggctgaatt tcagagcaca
     9241 ggatccacac cactggcgcg gcgctcatcg cataaccggc aatacacatc atcgtcatgc
     9301 caacagagag ccaggacaag ccactggaag ccgtaccgtt atcaaactgc atcaggcaat
     9361 agcccagcac cagagtgcct aacgccatca cgctgaaacc aattttcaga gccggtttac
     9421 gacctgcttt atctaccgta aagaccgcaa tgaaggtcgc gaacataaag gtcagtccca
     9481 cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa gcccgccatt ttgaagatac
     9541 gcggcgcgta gtacatgatg atgttcatac cggtaaactg ctgcatcgcc tgcaacaaca
     9601 taccgagaaa cacagcacga cggacgttac ggttgatctt aaacagtgcc caaccgccct
     9661 gttttaactt caggctttca cgaatttcgt tgagttcttc tcgcgctttt tccgacgtat
     9721 cgcgcagcat acgcaacact tcttccgcct caatatgacg ccccttttcc gccagccagc
     9781 gcgggctatt tggcaggaag accaccagaa taatcagcag aactgctggt aaagcaagaa
     9841 ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata aaaacgccag
     9901 cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc gaacgtactt
     9961 gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc
    10021 accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg
    10081 agtacaaaca ggatggcccc aggcggaacg acagccaacc attaaacagc gcaccaattg
    10141 ctgcgccgag catcatgcta ctaaccaccc attcctgcaa acgactggtc agcacaaagt
    10201 gatcggtaat gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc
    10261 ctgcgaccgc agcagctacc gaaacaaaca tattcatacg ccgcgtatca cgcaaagaac
    10321 gtggcgttaa agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa
    10381 gacattgtga aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa cagcataaat
    10441 atggattaaa ttgctgcgac atgccgttat gtgatggata ttccaatttt aaaattaagt
    10501 tgaattattg agattattat taaccaccta attttaaagt agataaaatt cataaagttc
    10561 attaattgat aattgatatg gattatttca taaccatgat atggattatg atgatctaga
    10621 gatataaaaa accctgccat gcggcagggt cataaaagta agaggaatga attaacgcgc
    10681 cagccaaccg ccatccacag caatggtata accatttaca taatctgaag cgctggaggc
    10741 aagaaacact accggcccca tcaggtcact cggcagtccc caacggccag ctggaatgcg
    10801 atcgagaatt tccgcgctac gttgttcatc tgcccgcagt tgttgggtat tgttggtcgc
    10861 catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgtttgccat
    10921 caatcgcgtc acacccatca ctccgctttt tgatgcggta taagaaggca cacggatccc
    10981 gccctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc cttgcgcgat
    11041 aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac
    11101 atcgtcccag tctttttcgc tgaactcgag agcgtcttcg cggcgaatca atccggcgtt
    11161 attcaccagg atatcaatat gaccaaattc agctaccgcg cgatccagca gtccaggaat
    11221 gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca gcgccgtgac
    11281 ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg
    11341 cgccagcccc aacgccatcc cctggcccag cccagtatca caaccagtga cgaccgcaac
    11401 tttaccttgg agagaaaatg cacttaaaat cataacaaca ccttattcgt ttatgcccac
    11461 aactagcgca aatctttaac ggccacatgg tccatatcat taaagacctg gttttcaccg
    11521 accatgcccc agataaaggt ataagctttg gttccgacgc cggaatggat cgaccagctc
    11581 ggggagatca ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc
    11641 atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg
    11701 cgctcgtggg tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc
    11761 cccatactca attgacaggt ttccagtaca tccgggacaa aatatttgtt aatcgtgcga
    11821 cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt
    11881 ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg
    11941 gtatcaatac tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg
    12001 tgaccgattt catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata
    12061 acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg
    12121 ccaacggaaa ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg
    12181 ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattacgc
    12241 agcccttggg tatccagcgt ttttgcgtgc gcaccgtgga tgctctgtct tacgtccaca
    12301 tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt
    12361 tttctctata aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat
    12421 gttttatttt taatttaagg attaaaaaaa gtgcagtgtc gatcacgaat atgtcatgag
    12481 aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac
    12541 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc
    12601 aatggtcaat gccacgccct agcccccgcc gatacaaagc gttgccagcc ctttgcgggc
    12661 attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat
    12721 cgggtgaccg agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag
    12781 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
    12841 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
    12901 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
    12961 taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt
    13021 tatggatgat gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg
    13081 ggctaaacct tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
    13141 ctgaccgtta ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
    13201 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
    13261 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
    13321 ccacaaccca tcatgcacca gactgtcaac caactggcta ttgccaagct gtgcaccggt
    13381 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
    13441 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagaccgga
    13501 accgcaaacg tcattaatcg tgattgcaga aacgctgtta ggaagaccac ctttaatagc
    13561 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
    13621 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc
    13681 aagttccacg gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa
    13741 cgccccgaca accacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
    13801 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga
    13861 aatatgcctg agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc
    13921 accaatagat gggtagatag ggcgttaatc tcccatactt aacttggttt atggtaaatt
    13981 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta
    14041 aagaagaagc tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctat
    14101 tccttcctat tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg
    14161 cctggccttt aacatattag ccacataaag ccttatgcca gttcatcctc tcatcaaaaa
    14221 catcagcagg tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta
    14281 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
    14341 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
    14401 gtatgttggt gagtctgggt gttgtattaa ttctgaatct catttttctg atgggacgtc
    14461 atgccactat tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc
    14521 tttcca
//
LOCUS       DAJTRQ010000101        14518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000101 DAJTRQ010000000
VERSION     DAJTRQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            84..365
                     /locus_tag="OO332_003030"
     CDS             84..365
                     /locus_tag="OO332_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943432.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="OO332_003031"
     CDS             complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="OO332_003031"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5943433.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="OO332_003032"
     CDS             complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="OO332_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5943434.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3462..4832)
                     /gene="purB"
                     /locus_tag="OO332_003033"
     CDS             complement(3462..4832)
                     /gene="purB"
                     /locus_tag="OO332_003033"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5943435.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="OO332_003034"
     CDS             complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="OO332_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5943436.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="OO332_003035"
     CDS             complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="OO332_003035"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5943437.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="OO332_003036"
     CDS             complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="OO332_003036"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5943438.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="OO332_003037"
     CDS             complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="OO332_003037"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5943439.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7969..9219
                     /gene="icd"
                     /locus_tag="OO332_003038"
     CDS             7969..9219
                     /gene="icd"
                     /locus_tag="OO332_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5943440.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9322..9645)
                     /gene="iraM"
                     /locus_tag="OO332_003039"
     CDS             complement(9322..9645)
                     /gene="iraM"
                     /locus_tag="OO332_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5943441.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9898..9942)
                     /locus_tag="OO332_003040"
     CDS             complement(9898..9942)
                     /locus_tag="OO332_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943442.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10178..10288)
                     /locus_tag="OO332_003041"
     CDS             complement(10178..10288)
                     /locus_tag="OO332_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943443.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10341..10745)
                     /locus_tag="OO332_003042"
     CDS             complement(10341..10745)
                     /locus_tag="OO332_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR5943444.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10966..11697)
                     /gene="bluR"
                     /locus_tag="OO332_003043"
     CDS             complement(10966..11697)
                     /gene="bluR"
                     /locus_tag="OO332_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR5943445.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11902..13113)
                     /gene="bluF"
                     /locus_tag="OO332_003044"
     CDS             complement(11902..13113)
                     /gene="bluF"
                     /locus_tag="OO332_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR5943446.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13427..13663
                     /locus_tag="OO332_003045"
     CDS             13427..13663
                     /locus_tag="OO332_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR5943447.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13706..13978
                     /locus_tag="OO332_003046"
     CDS             13706..13978
                     /locus_tag="OO332_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR5943448.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14007..14273
                     /gene="ariR"
                     /locus_tag="OO332_003047"
     CDS             14007..14273
                     /gene="ariR"
                     /locus_tag="OO332_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR5943449.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         3890 a         3319 c         3398 g         3911 t
ORIGIN      
        1 gcatgaggat tttgcttttg atataagcga cagccaccgc cagaaactgg aagaatttag
       61 cggggtgtaa tgaggtcagc agcatgacag aagccgaaat aacgggatta atccgccgcg
      121 ttgccggaat cagccagcag gctgacgaac aggccacgca gccggacagc gtgatagccg
      181 aaaactatgc gcgtgttgtt gctgaggtga tgcgccgtga tggtatagaa cttaatggcg
      241 tggatatgcg caacatacga accagagttc ttgagttgct ggcctaccgt cgccgcgtgg
      301 agatgtatcg ggagaaggag aaaatcacgt accacgggaa gaagccggag cggttacggc
      361 ggtaactcac tgagattaaa gcaaagcgca aaattgcgct ggctacatcc ctcacatctg
      421 aggactgtta accagcaggc cggaatatat tcagaaacta cgaaattttc gcagtttaat
      481 tactcgtcag cttaatcgct aacccgctct gatattttcg gaaacctcaa tttgaggaag
      541 tcggcgaggt aactgtttga tattcccgat agcgcaaaaa tgcgcggtcg ggcagaatga
      601 ccaccacctc aatcgtgacg gagagtgaca accagtgaca caccgtgaca aaaagacggt
      661 ttaaccgttc attaaaaatc gacgtgattt tgtgctttcc ggatgacgtg gccaacgtca
      721 ttttttagcg aaaaattctg attaaggtca ctggctgaac ggtcataatg accacgcaga
      781 ccatcgaggc aaccatagcg atcatcgtta cggttggagc ttttccgcca ttggtggaaa
      841 aggtgcacat ctggatcatc attacggtga tcaagggtat gtaaaaccca ccagcctgat
      901 taaaggttaa ccggaaaaaa aatccggtag ccccgttatg gggatcccca tatcgacatt
      961 aacgcccctc atgaattggt gcgcttcccc ctggaaagat tacccgcctg tatatttctt
     1021 gtgtctattt gttccacgtt gtttcataca gtgcaccgaa cggtgtagtt aatggtgtag
     1081 tcaatttgcg atttttggca ctttttcaat gaaaattgtt taatcgaatc aatgacttga
     1141 tgtagtggta aaaggacata tttattcatc tttcggcgca gaatgctggc gaccaaaaat
     1201 cacctccatc cgcgcaccgc ccagcatgct ctctccggcg acgattttac cctcatattg
     1261 ctcggtgatt tcgcgggcta ccgccagccc tacaccttgc ccagggcgta aagtatcaac
     1321 ccgttgacca cggtcgaaaa tgacctctcg cttgcttaat ggaataccgg ggccatcatc
     1381 ctcgaccaca atatagagat gctcgtcggt ttgccttgca gaaatttcga caaactcgag
     1441 gcaatattta caggcattat ccagcacgtt gcccatcacc tcgacaaaat cgttctgctc
     1501 accgacaaag ctgatctctg gcgaaatatc gagagagata ttgacccctt tgcgttgata
     1561 cactttgttc agcgctgagg tgagattgtc cagcagtggg gcgaccggat gcagctcgcg
     1621 gctgagcaat gtcccgccgc gcatactggc acgatgcagg tagtagccaa tttgctgtga
     1681 aatgcggctg atttgctcca gcattaccgg ctcagcatca ctgacgctca tcttttcact
     1741 acgcagagaa cgcagcgtac tttgcagcac cgccagtggc gttttcagac tatgggtcag
     1801 gtcggtgagc gtcgtacggt atttgtcgta acgttcgcgt tcacttttta acaatcggtt
     1861 caggtttcgt accagactgg tcagttctcg cgttgtggct ggattgagca attcgcggtt
     1921 atgttcttcc agttcgcgga cttcttttgc cagggcttcg atggggcgta aactccacca
     1981 ggcggcgacc cacagcagcg ggatcactaa cagcagattg gctgagagta catagataaa
     2041 ccagctccag accatatagg aactttttag ctccaccgga atggtatcca ccaccacaat
     2101 ggttaatttt ggcatccgcg atgttgccgg gtagacgttt accgccaccg agtgggtcat
     2161 ctccgcgtcg tcatcatctt cccgcacttc ctgcaactgt tgctgtatcg aatgatctcc
     2221 actcagcaag aggctggtat cgttaacatc cgcttcaatt tcatgaaaac cattcgattt
     2281 cagccagtca ggctggatca ttttcatcag ccagggcacg tcacgttgcg cccataaaag
     2341 ctgcccgttc tcatcataaa ttagcgtcat ggtggggctt tgcttgtcga tattttcggg
     2401 taactcgaca tgcaacttat tgttttccca ctgcgcaagg gtatagaaca gattgctctc
     2461 gccacgtaac agccgaaacg tagttttatc gaaactgacg ctataaccga tcagcgcgac
     2521 cattccgtag gcaagcgaaa gcaccagtac taccgctgcc gttgccaaca gaaaacgtac
     2581 ccgcagcgag agcgggaaaa aaagacgcag taattttttc atcagcgcaa ttcgaacagg
     2641 tagccctggc cgcgaacggt ggtaatgact tcttgcggat attgtgcctg aatttttttg
     2701 cgcagacgtc ccatcagtac atcaatggta tggctttccc gcagctccgc atccggatag
     2761 agttgtagca ttaacgaatc tttgctgacc actttgccat tattgcgtat caacgtttcc
     2821 ataatggtgt attcgaacgc ggtcagtttg atcacttcgt cattaataga taattcacgg
     2881 cgagagagat caacctgaaa cggggggagc gaaatgacct gtgaagccag accgctatta
     2941 cgccgcatta atgcctgcat tcgcgccatc acctcttcaa tatgaaacgg tttagtcacg
     3001 taatcatcag caccggcact taatacttcg actttgtcct gccagctttc acgggcggtt
     3061 aataccagaa tcggcagtga aacatcgttg ctacgccagc ggcgaatcag tgacagaccg
     3121 tcctcgtcag gcaatccgag atcgacaatc gcaatatccg gtaaatgttc attgagataa
     3181 taatcggctt ctttggcatc ttctgcgtca tcgacctgat gaccagcatc ctgaatctga
     3241 actttaaggt ggtgacgtaa caacgcattg tcttcaacaa ccagtacgcg catttttatt
     3301 tctccctgtc ttaattatta aaatagtgta acgcgattat gtgtttatgg gggtaaacat
     3361 taaataaacc agcggggagg ggaggtaaag tgaaaaaaga aaaagtggat aatcttaata
     3421 agcaggccgg acagcatcgc catccggcac tgatttgagg cttatttcag ctcatcaacc
     3481 atcgtgatag ctcgaccaat atagttagcc ggcgtcatcg ctttcaggcg ggctttctct
     3541 tcttctggca acgccagacc atcgataaac tgcttcatgc cttcggcgtc aacgcgctta
     3601 ccgcgagtaa gctctttcag cttctcgtac ggtttttcga tgccatagcg acgcataact
     3661 gtctggattg gttctgccag cacttcccag ttgtgatcca gctcatccag cagatggtca
     3721 cggttcactt ccagtttgct cacgcctttc agggtggatt gatacgcaat cagcgcataa
     3781 ccgataccca cgccgaggtt acgcagcacg gtggagtcgg tcaggtcacg ctgccagcgg
     3841 gaaaccggca gtttgcttgc cagatgctgc aataccgcgt tggaaaggcc cagattccct
     3901 tcggagtttt cgaagtcgat cgggttaact ttatgcggca tggtggaaga accaatctca
     3961 ccagcaatgg ttttctgttt gaagtggtta agggcgatat aaccccagac gtcacggtca
     4021 aagtcgatca gaatggtgtt gaagcgcgca acgcaatcaa acagttcggc aatgtagtcg
     4081 tgcggttcaa tctgggtagt gtacgggttc cattgaatac ccagcgaggt gacgaactct
     4141 tcgctgaact gatgccagtc aacttccggg taagcggcga tgtgggcgtt atagttaccg
     4201 accgcgccgt tgattttgcc gaggatctcc acctggttaa gctggcggtg ctggcgctcc
     4261 atacggtagg cgacgtttgc catctcttta ccgatggttg acggcgtggc tggctgaccg
     4321 tgggtacgag acagcagcgg gatatcgcgg tactgaacgg cgagatcttt aatgccatca
     4381 atcagttgac gccagtacgg caggatcact tcatcacgcg cggttttcag cattaatgcg
     4441 tgggagaggt tattgatatc ttccgaagta caggcaaagt ggatgaattc agaaaccgcg
     4501 tgcagttccg ggatctccgc cactttttct ttcaggaaat actcaaccgc tttaacgtcg
     4561 tggttagtgg tacgctcgat ggttttgatg cgtgcggcat cttcttcgct gaaattggcg
     4621 acaattgcat caaggtaacc gattgcgtcg gcagcaaaag caggaacttc cttgatcgct
     4681 gcgtgcgcgg ccagtttttg cagccaacgt acttcaactt gtacacggaa tttcagcaaa
     4741 ccatattcgc tgaaaatccc gcgcagcgcg ctgagtttat cgccgtagcg tccatcgaca
     4801 ggggaaacgg cggtcagtga ggataattcc atagatcaca actccggggt taaatgagca
     4861 agaatttgtt ttgcctgagt ggtcaggcga ttacgagaaa acatcagttg tagacgtccg
     4921 ccgccgacct ggtgccagag cacggcggcg cgaatgcctg ccagcagggt tgcgcgaact
     4981 ttcgcctgca cttgtgggct ttgcagtaca gcaggggaac cggtgacctg aatgcgcggg
     5041 ccaagcgggc tgatcacatc aacatagata gcagccatcg cgctcatcag cgtttcggac
     5101 tgtaaatcga agtgttcgag ctggcgttgc aggccgttga tccggttgcc cagagtgtcg
     5161 agcgcgcctt tcgctgagga gagtttgcgc tcaagcacca tcaagctgag tgtgtagcgg
     5221 gttaattcgg cgtttaagcc ctggcaactg ctggcattga gcacgccgag cagggtttcc
     5281 agcccgacgc gcaggttggc ttcgctaccg ccaaaaaccg ccagcgtcga gctggggttc
     5341 atatcaataa tactgttgag tgagacgtgt agcgcatcgg catcacaatg cccctggtga
     5401 gcgagttgtt gcaccaggcg tgccgactga caaataccgg ccagggcgag ggtgatgtca
     5461 tagtaattct ttgccacgtt cactgcttcc ttgttttaag taaagataat aatcagaccg
     5521 gcagcggcag acgctgctca ataataccgc caccgaggca cacttcaccg ttatagaaga
     5581 cggcagactg gcccggcgtc acggcggcaa ccggctcatc gaaaatcact ttaatgcgat
     5641 cgtcgtccag cgccttgacg gtgcacggaa tgtcggtctg gcgatagcgg gtttttaccg
     5701 tgcaacgcat agtgccggtg aatggttcgc gatcgaccca gtgcagctgc tgggcaatca
     5761 acccgacaga catcagccgc gggtgttcat ggccctgagc gacaaccaga atgttgtttt
     5821 cgacgtcttt gtccaccaca taccacggtt cttcggtgcc ttctttggtg ccaccgatac
     5881 ccagaccttt acgctgaccg agagtgtgat acatcagccc ctggtgctca ccaatttcat
     5941 ggccatcgac ggtaatgatt ttgcccggtt gcgccgggag ataacggccc aggaactcgc
     6001 ggaatttacg ttcgccaatg aagcaaatgc cggtagagtc ttttttcttc gcggtgacca
     6061 gaccaagatc ttcagcaatc ttacgcacct gcggtttttc cagttcgccg accgggaaca
     6121 ggctttgcgc aatctgctca tggctgagcg tataaaggaa gtagctctgg tctttattgc
     6181 tgtccagacc acgcagcagg cggctcttgc catcgacatc ggcccgacgt acgtaatgac
     6241 cggtagcgat ataatcggca cctaaatctt cggcggcaaa ttcgaggaag gctttaaatt
     6301 tgatctcctt gttgcacaga atatccggat tcggcgtgcg accggcttta tattcggcaa
     6361 ggaacagttc gaagacgttg tcccagtact cggcagcaaa gttaacggtg tgcagttcaa
     6421 tgccgagctt gtcgcagacc gcctgggcat cagccagatc cgctgccgct gtgcaatatt
     6481 cctcaccgtc gtcttcttcc cagttcttca taaacaggcc ttcgacctga tatccctgtt
     6541 gttgcaacag ccaagcagaa acggaggaat cgacaccgcc ggacatgccg acgattactt
     6601 tttttgcggt ttcagacatt ggatcactca cgacattgaa cttcaaggcg gcgtattcta
     6661 tcacgcagcc ttttagatga caccctttgt aaaaggccag ttaaaatcgc caatcatctc
     6721 cagcggataa cgttgcccgc tttgataaca acgaatactt tccgccacca gcggcgaacg
     6781 aagatttgac gcctgtaaaa tttcttcggc gctgacccaa cggcagcagt cgatatcgct
     6841 gtcatgaggc tgggtcgggc atatttgctc aagctcaatg gcaaagagga aacgcaaaaa
     6901 cggcgtttta tctggcgcaa tccactgatg catacgaata aagtgttgcg gctgcgcgct
     6961 gatgccggtt tcttcccaca gttcacgggc ggcggcttcc actaaggttt catcggcttc
     7021 cagatgcccg gcaggttggt tccataacgc tttaccatta atcgtctctt caacgactaa
     7081 aaatttgcct tctgcgtgca ccacgcaagc aacggtaacg tgcggtttaa acattttgca
     7141 tccttaatct gtcacttctc gccattcgcc attggcaaga ttatccaaag agtaatctcc
     7201 catcgcatag cgaatcagtc gcagcgtggg gaaaccaaca tgggcggtca tgcggcgcac
     7261 ctggcgatta cgtccttcat ataaggtgat cttcagccag ctggtgggaa tacttttgcg
     7321 ttcacgaatt ggtggattcc gcggccataa ccacgcgggt tcgtcaacca gctccgcgcc
     7381 tgcgggcagg gtagggccat catttaaggt tacgccattg cgcaaggctt caagtgcgtc
     7441 ttgtgtggga ataccttcca cctgcacata atagattttt ccggtgcgtt tacccggctg
     7501 ggttaaacgc gcctgcaacg cgccgttatt ggtcagcacc agcaaccctt cgctatcgcg
     7561 gtcaaggcga cctgctgcat aaacgccctg aaccgggatg aattctttta atgttttgcg
     7621 tccggcttca tcggtgaact gcggaagaac atcgtagggt ttattgaaga ggatcacacg
     7681 cgtgggctgg ttttcatgtt tacgcctggt agaacgttgc gagctgaatc gcttaacctg
     7741 gtgatttcta aaagaagttt tttgcatggt attttcagag attatgaatt gccgcattat
     7801 agcctaataa cgcgcatctt tcatgacggc aaacaatagg gtagtattga caagtcaatt
     7861 acaaatcatt aacaaaaaat tgctctaaag catccgtatc gcaggacgca aacgcatatg
     7921 caacgtggtg gcagacgagc aaaccagtag cgctcgaagg agaggtgaat ggaaagtaaa
     7981 gtagttgttc cggcacaagg caagaagatc accctgcaaa acggcaaact caacgttcct
     8041 gaaaatccga ttatccctta cattgaaggt gatggaatcg gtgtagatgt aaccccagcc
     8101 atgctgaaag tggtcgacgc tgcagtcgag aaagcctata aaggcgagcg taaaatctcc
     8161 tggatggaaa tttacaccgg tgaaaaatcc acacaggttt atggtcagga tgtctggctg
     8221 cctgctgaaa cccttgatct gattcgtgaa tatcgagttg ccattaaagg cccgctgacc
     8281 actccggttg gtggcggtat tcgttctctc aacgttgccc tgcgtcagga actggatctc
     8341 tacatctgcc tgcgtccggt acgttactat cagggcactc caagcccggt taaacaccct
     8401 gaactgaccg atatggttat cttccgtgaa aactcggaag acatttatgc gggtatcgaa
     8461 tggaaagctg actctgccga cgccgagaaa gtgattaaat tcctgcgtga agagatgggc
     8521 gtgaagaaaa ttcgcttccc ggaacattgc ggtatcggta ttaagccgtg ttctgaagaa
     8581 ggcaccaaac gtctggttcg tgcagcgatc gaatacgcaa ttgctaacga tcgtgactct
     8641 gtgaccctgg tgcacaaagg caacatcatg aagttcaccg aaggcgcgtt taaagactgg
     8701 ggctaccagc tggcgcgtga agaatttggc ggtgaactga tcgacggcgg cccgtggctg
     8761 aaagttaaaa acccgaatac cggcaaagag atcgtcataa aagacgtgat tgctgatgca
     8821 ttcctgcaac agatcctgct gcgtccggct gaatatgatg ttatcgcctg tatgaacctg
     8881 aacggtgact acatttctga cgctctggca gcgcaggttg gcggtatcgg tatcgcccct
     8941 ggagcaaaca tcggtgacga atgcgccctg tttgaagcca cccacggtac tgcgccgaaa
     9001 tacgccggtc aggacaaagt aaaccctggc tctattattc tctccgctga gatgatgtta
     9061 cgccatatgg gctggactga agccgcagac ctgattgtta aaggtatgga aggcgcgatc
     9121 aacgccaaga ccgtaaccta tgacttcgag cgtctgatgg aaggcgctaa actgctgaaa
     9181 tgttcagagt ttggtgatgc gatcatcaag aatatgtaat cactacatgt gttaaatatt
     9241 taaacgggcg tataacacgc ccgttgtttt atttatgtgg atattattaa tagcatatcg
     9301 agcatattta tataaaacct attacttgag cccatatggg catattttta taatacaact
     9361 cttatgcaaa catttatttg ttatttcgct ttctcctgga ggacactctt gactgctttt
     9421 gagtaaactc cataaattct tgttgaatgg tgcaatgtta taaatagtaa taggatattc
     9481 tttttcctta aggataatac caagcttaac cggtgtaaat atccttccag gaggaagaaa
     9541 tatagtagat tgataccaga tgatcatttt catatcaccc catatggctg aaaaagatat
     9601 gccgcatgta ggttgaatta ccgtgtcaat tactacccac ttcatttgtt atgtcttatc
     9661 ccacggtatt taatatagtt catttggatg ttcatttctt tattttgcat atgagtatat
     9721 taccccttca aaaaataaat taattaaaac gattgcttat ataaaacaaa atttaaatca
     9781 aggaatctca atggatgtta aacaaaatga gatttagtga aaacaataaa ttattcactt
     9841 cgttttagat ttgtttagct ataatgttat acattcaaat gactgaacat cctgtaatta
     9901 aaacatagcc tttatgctac ttggtgccaa tttgctaaac attatggttg cctttttata
     9961 taacgataat aatgaatata aacatgacat gagaataagg tttcaatttt tgagttatat
    10021 aggaaaggtt taacctgttc ctggctaaaa tacatataac tggatgatga ctaaaccaaa
    10081 acacatatgc gttaagtatt gaaacgggcg tatggcacac ccgttgtttt tataaatata
    10141 ttaaccgtta taaaataacg tataaaaagt caagtgatca catttcaaat atcaatttga
    10201 tagtatgggc ttggtgatta tttatgggta gcaataaaag gacagtattt atcatccata
    10261 gggatagtct ctgtactttt attcccatta tgctaatgcc ttactgaatt atgaagcatt
    10321 tcttaagcat ccaactttag ctagattaat ggtttattat tttctacatc ttcaatatat
    10381 aaaagcgtat tatcaatggc gtagtaactg cgtttgttat gattaacatc agtaacccaa
    10441 cggaaaacgc ccgcgcctgc tagtgttgaa cagtattccc gaaatgtaga ttttccgcaa
    10501 atatgaagca atgcggcctc ttttatttta gcaggattct tggtcgtact aacttttaac
    10561 tggttcctgg ttcctcttaa taacaaaacc gtgtcatcgt gagtaataat tctgatgtta
    10621 tccgtagcca gataataaat gtaatgtgca atacggtgat gttttaattc tgaataaaac
    10681 caggagaagt tttgctcttt tctcacttgc tcaaacatct tttgaaaaac aacgacctga
    10741 tccatcagga taataacctc ttgttagttg tgagactgcg tagtgtgcac gatcggtttt
    10801 accacttcaa tctggtctgt cctttggctg tgatatgtac agagtgtgat agagggatta
    10861 cctgaattct cccggtgagc attttgcaac ggaccagctc cggtacaaat gctgttgtgg
    10921 gttcagatta taacattctg tctaaggggc gggataaagg tgaaattagg gggcatgaaa
    10981 gatgacttta taaccttgct caccccaatg ttgtaaaagt tcgttttgcc ttctcgttgg
    11041 tgccatgcct gtccagacaa tcaatgtttg cgtcgggaac agttccgggc gcggcgattc
    11101 aatgggatca gcaagaacag aaatgtgcca tcctgacaat gataaacgcc atgcttccag
    11161 ccacaatcgt gctctctctt caacattcca cgccatcaga atggcttctt taccgggctt
    11221 acggcgcgtt tcgaaaagcg aggttgctgc gtattcaatt aatgcgccgt caaacatact
    11281 gctcataatg cgggaggtgt tgtgatcaag cacgagacgc tggcgaacag gaaggtaaac
    11341 atgattaatc aattgatcta ctgggtactc tcgacccagt gaaataattc tcgcgcgtag
    11401 tttggcagga ttagccatac gaagaattga catcatctct tcttgcaggc ggctccagtc
    11461 atcttccgta tcctggctgg tggtttccag taatgcttta actttgccta cagggacgcc
    11521 attactgatc caacgcttga tctcttcgat gcgttgtatg tcttcttcat caaagagtcg
    11581 gtgtccgcct tcactgcgct gtggttttaa caaaccgtag tggcgttgcc aggcccggag
    11641 agtgacagga ttaatcccgc aacgttcagc aacatcacca atgctgtaat aagccacaat
    11701 tcctccttgc ggtcacaaat ctccgtcgcc ggtacacgac ccaataatac tttgtacaat
    11761 atacgctaaa attgtacaaa gtataaataa gatttcagct aaattggatg aaacattatt
    11821 tttaatgtag attaaattta aacataacgt attcattttc acgatgattt actgaaatca
    11881 tgtgaaagaa tgtgctgaaa attatttttt ctctggccac gctatggaag ggataccatt
    11941 caatttagct ttagcaaaca gatctccctg aaacatctca attcctgcgg attcaagcca
    12001 catccactct tctggtgttg ccacgcccat agcactgact tgaatttcaa gtgatgtaca
    12061 gcattttatg atcgcttgaa taattgcctg ccgtggcccg cttttatgaa cattggtaat
    12121 caattcctga ctgattttaa ttctgtcagg ctggaagcgt gacaggagta acaaaccggc
    12181 aaaacctgcg ccaaaatgat caattgctac actgataccg gcagccttaa gcgatttaat
    12241 cgcttctgca aactcatcaa accgagatat aacttcactt tcagtaaatt caacgatgat
    12301 ttgttcaggc accagagcat tggcctttat ttcatcaagt aaaaaagaga ctgcgtcagg
    12361 ttcgttaacc agggtcatag gtaatagatt gattgaaatc attttatcac cgagctcaag
    12421 tgcgtgtgtc atcgcgaatg caagcgcctt acttttgaga tccgctgtgt agatttcccc
    12481 gtctttacgc tgcccaaccg ctatggctga tgggctatct tcattttttt gcacaatggc
    12541 ttcaaaagcg attatccgcc gcgataaggg atcgacaata ggatgaaagg caaagtggtc
    12601 gtttatagtt ggtgaaaggg tacaggaatc aagttcttta tcagatccgt cagcgataaa
    12661 aagccaggag tcttcggcag ggatctcgaa ataggttgat tgttcggttg caaggacaaa
    12721 agtacgaaaa aattgtagcg ctctgtcatc ataagttagc tgaaattttg atgtgccttt
    12781 gtcgaatacg gcctgtaaaa cgtcatctcg ctcgtgcagg cgcaaatcaa ataattccat
    12841 tcccgctttg ccaaaacggc gagcaggcgc gtaatcgcac agcagctcaa caatattata
    12901 gtgccgtgga tcttggcata tagcccgata tatcatttta acctgttctt ccggaccttc
    12961 cagaagctgg aaaaaatgag aaccattaaa cagtaagatc cctgttacgt cagactgcat
    13021 gttcctgcga tttgctatcg aaaccatttc ttcgattttt ttgacaggtt cgtcgtcacg
    13081 tatatggcta cgataaataa gggtggtaag cattaacaat ccagggtaat gggtgaggcg
    13141 agagtaagac ggtaacagac atatcttctt gtgtctttct tttaatacca aaacataacc
    13201 gtttctttac attgataaaa aatggaaaaa gttgaacacg agttggcgaa aaatcttgta
    13261 tagattgtca gttaaatgat gcaatatgtt ttatcataac acattgtttt atatacatta
    13321 gtactaattg caaaaaatta atttatcatt ctgtacacat atttcgtaca agtttgctat
    13381 tgttacctca cttcacattg attaacattt ttaacagagg cgtagcatgc atcaaaattc
    13441 agtgacttca gattctgcgg gagcaatcac tcgttacttt gcaaaagcta acttgcctac
    13501 tcagcaggaa actctcgggg aaattgtgac tgagattttg aaagatggac gtaatctgag
    13561 tcgaaagtcg cattgtgcca aacttctttg ccgactggaa caagcgacag gggaagagga
    13621 acagaaacat tataatgcac taattgggtt gctttttgaa taatgcaaca gcctgattat
    13681 ttaacaggtt agctatgaag tcgttatgaa gacatctgat aatgaacgta taaaatatga
    13741 aattactggc caggcggtgc tccagatact gcgcatgaag ataaattttt cattgcagac
    13801 tcttattaag caattactcg taatgaaatc ggctgaggaa gatgctttcc gacgcgattt
    13861 aattgacagc ataattcgtg attttagcaa tagtgattca ggagggccaa accgaagaac
    13921 agcgacagct gacaataaaa gtatgttcaa tggtaagaaa ataaacagaa tacattaaaa
    13981 tttcataagt aagatgagag gttaccatgc ttgaagatac tacaattcat aatgcaataa
    14041 ctgataaagc gttagcaagt tactttcgca gttcgggtaa tttgttagaa gaagagtcag
    14101 cagtgttagg gcaggctgtc accaatttaa tgctttcagg cgataatgtt aataataaaa
    14161 atattatctt aagtctgata cactccctgg aaacaacaag tgatattctc aaagctgatg
    14221 tgattagaaa aacactggaa atcgtgttgc gatacacagc tgatgatatg taacttctaa
    14281 ttattaagta taagtttata gaaaactcat tcatcttatt tttgtctgtc gctttagact
    14341 atacacagca taattttatt gggttaatat ttctacgagg ctgacatgaa taattcaatc
    14401 ccagagagat ttatttttca atgtgctttg tttaaaaatc tcgagaggga ggtgttcatg
    14461 acgcatgggt atgttgatag tcatattatt gatcaggctt tacgcttgcg tttaaaag
//
LOCUS       DAJTRQ010000102        14002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000102 DAJTRQ010000000
VERSION     DAJTRQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..172
                     /locus_tag="OO332_003048"
     CDS             <1..172
                     /locus_tag="OO332_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5943450.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            277..1629
                     /gene="celF"
                     /locus_tag="OO332_003049"
                     /gene_synonym="chbF"
     CDS             277..1629
                     /gene="celF"
                     /locus_tag="OO332_003049"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5943451.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1642..2400
                     /gene="chbG"
                     /locus_tag="OO332_003050"
     CDS             1642..2400
                     /gene="chbG"
                     /locus_tag="OO332_003050"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5943452.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2447..4708)
                     /gene="katE"
                     /locus_tag="OO332_003051"
     CDS             complement(2447..4708)
                     /gene="katE"
                     /locus_tag="OO332_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5943453.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4891..5154
                     /gene="cedA"
                     /locus_tag="OO332_003052"
     CDS             4891..5154
                     /gene="cedA"
                     /locus_tag="OO332_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5943454.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5431..6247
                     /locus_tag="OO332_003053"
                     /pseudo
     CDS             5431..6247
                     /locus_tag="OO332_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6251..7641)
                     /gene="tcyP"
                     /locus_tag="OO332_003054"
                     /pseudo
     CDS             complement(6251..7641)
                     /gene="tcyP"
                     /locus_tag="OO332_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7774..8364)
                     /locus_tag="OO332_003055"
     CDS             complement(7774..8364)
                     /locus_tag="OO332_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5943455.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8527..9194)
                     /gene="hxpB"
                     /locus_tag="OO332_003056"
                     /pseudo
     CDS             complement(8527..9194)
                     /gene="hxpB"
                     /locus_tag="OO332_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9341..9877
                     /locus_tag="OO332_003057"
     CDS             9341..9877
                     /locus_tag="OO332_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5943456.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9918..10778)
                     /locus_tag="OO332_003058"
     CDS             complement(9918..10778)
                     /locus_tag="OO332_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5943457.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10884..11174)
                     /gene="ghoS"
                     /locus_tag="OO332_003059"
     CDS             complement(10884..11174)
                     /gene="ghoS"
                     /locus_tag="OO332_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5943458.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11275..12204)
                     /gene="pfkB"
                     /locus_tag="OO332_003060"
     CDS             complement(11275..12204)
                     /gene="pfkB"
                     /locus_tag="OO332_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5943459.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12490..13248
                     /locus_tag="OO332_003061"
     CDS             12490..13248
                     /locus_tag="OO332_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5943460.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13301..13408)
                     /locus_tag="OO332_003062"
     CDS             complement(13301..13408)
                     /locus_tag="OO332_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943461.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13580..>14002)
                     /locus_tag="OO332_003063"
     CDS             complement(13580..>14002)
                     /locus_tag="OO332_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943462.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSRANIMDLL
                     KGATAQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3612 a         3218 c         3458 g         3714 t
ORIGIN      
        1 gatccgtatt aattttgcca aaaaacaact ggaaatgacc aactattccg tgacggatat
       61 tgcgtttgag gccggttata gtagcccgag tttgtttatt aaaacgttta agaaattaac
      121 gtcctttacg cctaagagat atcgtaagaa attgactgaa tttaatcagt aagtcgttat
      181 acctgacaat tcacatatct gtcctgttgc tggattattt atgtccgggg gggcagatat
      241 gccagatatc agtattctgt actgaaggga gaaattatga gccagaaatt aaaagtcgtc
      301 actattggtg gcgggagcag ctataccccg gagttactgg aaggatttat taagcgttat
      361 cacgaattgc cggtcagcga attatggctg gtggatgtcg agggtggcaa agctaaactg
      421 gatattattt tcgatctctg ccaacggatg attgataacg ctggcgtccc gatgaagctt
      481 tataaaacgc tggattgccg cgaagcattg aaagatgctg atttcgttac tacccaactg
      541 cgcgttggcc aattaccggc gcgtgaactg gatgaacgta ttccattaag tcatggttat
      601 cttggtcagg aaaccaacgg cgcgggcggt ttgtttaaag gtctgcgtac cattccggtg
      661 atttttgaca tcgtaaaaga tgtcgaagaa ctttgtccga atgcatgggt gattaacttc
      721 actaacccgg cgggaatggt tactgaagcc gtttatcgtc ataccggatt taaacgcttt
      781 atcggcgtgt gtaatattcc gatcggcatg aagatgttta ttcacgatgt tctgatgctg
      841 aaagacagcg atgatttatc tatcgatttg tttggcctca accatatggt gttcattaag
      901 aatgtgctgg taaatggcaa gtcgcgcttt gccgaattgc ttgatggtgt ggcatccggg
      961 cagttaaaag catctggcgt taaaaatatt ttcgatctgc catttagcga aggcttaatt
     1021 cgttctctga atctgttgcc gtgttcttat ttgctttatt acttcaagca aaaagagatg
     1081 ctggctattg aaatgggcga atactacaaa ggcggcgcac gagcgcaggt agtacagaaa
     1141 gtcgagaaac aactttttga gctgtataaa aacccggagt tgaaagttaa gccgaaagaa
     1201 ctggaacagc gcggtggggc ttattactct gatgcagcgt gcgaagtgat caacgctatc
     1261 tacaacgaca agcaagctga acattacgtt aatatcccgc atcatgggca tattgataat
     1321 attccggcag actgggcggt agaaatgacc tgtaagctgg ggcgcgatgg cgcgacgcca
     1381 catccgcgca ttacgcattt cgatgataaa gtgatggggc tgattcacac cattaaaggc
     1441 ttcgagattg ctgccagcaa cgccgcactt agcggagaat ttaacgatgt gttactggcg
     1501 ctaaacctta gtccgttggt gcattccgat cgcgatgctg agctgctggc acgcgagatg
     1561 attctggcgc acgagaaatg gctgccaaat tttgccgact gcatcgcaga gcttaaaaaa
     1621 gcacattaac cgaggctgat tatggaacgc ttactgattg ttaatgccga tgattttggc
     1681 ttaagcaaag gtcagaacta cggcattatc gaggcctgtc gcaatgggat tgtcacatcg
     1741 acgacggcgc tggtgaatgg gcaggctatt gaccatgcgg tgcagttgag tcgtgatgaa
     1801 ccgagtctgg ccatagggat gcactttgtc cttactatgg gcaagccact gacagctatg
     1861 ccggggttaa cccgcgatgg tgtgctggga aaatggatct ggcagttggc agaagaagat
     1921 gctttaccgc tggaagaaat tactcaggag cttgccagtc agtatttgcg tttcattgag
     1981 ctatttggac gcaaacctac gcatcttgat agccatcatc atgtgcatat gttcccgcag
     2041 attttcccga ttgttgccag gtttgcggct gaagagggga ttgcgttgcg catcgaccgt
     2101 cagccgctat ctaacgctgg tgatttaccg gctaatctgc gcagttcgca gggattcagt
     2161 agcgcgtttt atggtgaaga gattagcgag gcactgttcc tgcaagtgct ggatgacgca
     2221 agccatcggg gtgatccttc gctggaggtg atgtgtcatc cggcgtttat cgataatact
     2281 atccgtcaga gtgcttactg tttcccacgg ttaacggagc tggaagtgct gacatcagcg
     2341 tcgttgaaat atgcaattgc tgagcgtggt tatcggttgg gtagttatct tgatgtgtaa
     2401 gtaaggtggt aaggtcactc cccaggggac gtgaccttat ccatatttac gccggaattt
     2461 tgtcaatctt aggaatgcgt gaccacacgc ggtgttctgc catcagcgtt aacagttcat
     2521 ccataaaact accgtcggtg ctgtcagctt ccacaatccc ttcttcaccc tggtcagcta
     2581 ccttgattgt tgctttaaac ttgcgcgcgt ctcccgccag cgcaatcggt ttaaggtgtt
     2641 tgtaggcttc catcagatag tagttggcat cgccgttgtc agcgatatcc gcgatattgc
     2701 cgcaagggac aatgaccgca tcgaccgtca gcgaaggtgc accggcaaag gtagcggcta
     2761 taggcaacac cgtaccgtca tccgcagtca cttcacccat tcgggagtag agcagtttgg
     2821 catgaacgcc tttggccttc agggccttga gaatggcaag aaggtctgcc gatctcactt
     2881 catcattaag taaaatcgct accacgcgac ctttcacatc accgtcagga atggcgtaca
     2941 aacttaagga tggatccttt ttcagaccgt tgacgtccgg aggtggggtg atattcagct
     3001 ggtcgtcagt cagttcgata ccgagatttt tcgccaccgc ctgggccaga gtgagatcaa
     3061 tatgcgccag ctggtcaaca acgtgctcac gaatatacgg acgaacgact ttgcttaact
     3121 caaaactgaa accatcgaca atatggcgct gctcgaatgg cgtctgactt agccagaaca
     3181 gacgcggatg ggaataatat tcgccaaacg atgggctgcg ctcgcgaact ttattgcctt
     3241 ccacgcgctc ttggtatgat tcaaaaccgc cgcgtttcgg ccccggtggt gtttcgcgcg
     3301 gccagttatc gttgatcgag ttcggttcat aattcgccgg gttagtgtcg atccccatac
     3361 gatgcatgcc gtcacgctgg aaattatggt aagggcaggt cgggcggtta atcggaatct
     3421 catggaaatt cggcccacca agacgactga tttgtgtatc ggtataggag aacaaacgtc
     3481 cctgcaacag gggatcgttg gtgaagtcca gaccaggaac aatatgacct ggatggaatg
     3541 ctgcttgttc gttttcggca aagaagttat ccggattgcg attgagcacc attttgccga
     3601 cacgctgaac gggcaccagt tcttccggga tcagtttggt tggatcgaga agatcgaagt
     3661 cgaacttgaa ttcatcttct tcaggaatca actggaagcc cagttcgtat tccggaaaat
     3721 cgcctgcttc aatggcttcc cacaactcgc ggcggtggaa gtccgggtca cgtccggtga
     3781 gtttttgtgc ttcatcccaa acgagtgagg ctttacctgc cagtggtttc cagtggaaac
     3841 gtacaaacgt tgccttccct tcggcattaa tcaggcggaa ggtgtgaata ccgaagcctt
     3901 ccatggtgcg gtaactgcgg gggatgccgc gatccgacat cgcccacatc acgttgtgca
     3961 gagtttcagg ttgcagagaa acataatccc agaaagtatc gtgggcactt tgcccttgtg
     4021 gaattgccca gtgcggttct ggttttaccg catgaacaaa atcggggaat ttatgcgcat
     4081 cctggataaa gaagattggc gtgttattgc caacgaggtc aaaaataccc tcttcagtat
     4141 agaacttggt ggcaaaacca cggatatcac gcacggtatc ggcagagcca gcgccaccct
     4201 gaacggtaga gaacctaaca aatactgggg tgattttgtt cggatctgag aggaaatccg
     4261 ctttggtgat atcgcttaag cttttatatg gctggaaata accgtgagct gctgatccgc
     4321 gtgcatgaac aatacgttcc gggatgcgct catggtcaaa gtgggtgatt ttctcgcgca
     4381 gaataaaatc ttccagcagc gttggaccac ggttaccggc acgcagtgag ttttgatcat
     4441 cggcgatgcg cacgccctga ttagtggtca gcgcataatt ttcactgcct ttgcgtacgt
     4501 cttccagaga attaagtttt tcgttacgtg tatcaggggc tttcaggctc ccaggggcgg
     4561 taggttgtgc acctggcggt gttggttcag ccgctggacg atgagatccg tcctcaggtg
     4621 ccagtgagtc catccccggt ttcgcttcgc tggaatcgtg tagtggtgac tggtgctgat
     4681 gtgggttctt ttcgttatgt tgcgacattg aactcgtctc cttaatttat tactgaaagg
     4741 gccgctgttt tggtcaaaaa actctctaac tatagaccac cagccagatt ccgcttcgga
     4801 gactattctg gaaacggtgt tatgaaatac acgcaaagcg ggacgtacag gggctaaatc
     4861 ggctaaacta cagtttttct aatttttaat atgcgtttag tgaagccagt aatgaagaaa
     4921 ccgctccgcc agcaaaaccg ccagattatt agctatgtcc cacgcacgga acccgcgccg
     4981 ccagaacatg cgataaagat ggattcgttt cgtgatgtct ggatgctgcg tggcaaatat
     5041 gttgcgtttg tactgatggg agagtcattt ctgcgctcac cggcgtttac tgtgcctgaa
     5101 tctgcccaac gttgggcaaa tcagatccgc caggaagggg aagtgactga gtaaggatta
     5161 catacctcct ggttttgccg ggagttacag gacctgaaat tctgcatttc gttgctacat
     5221 tcattcaaac cacctgtaac aaaaagctgc ctttgacgag ctattgctat aaataagtga
     5281 tcttttttct ttaattgttt atttatttta aaatggttaa gttttgctaa tcatgatgag
     5341 ttatttttga tagttcatcc tgtggcgtcg ctcttaaact tatattgtaa atggcttaga
     5401 tttaaataag ataataattt tgttaacaat atgaggcggg agatgttttc tacaaactta
     5461 atacagagta attatggtga tttaaatatt aaaagcctgg ctttcgactc ttttaaagaa
     5521 aggcttgagt ctaccatggc ggcactcact ttctttatat caacgggtca atgtgactgc
     5581 gatgaaatag cagagtcgaa cttcaatcgt atgattgcct atatgtctaa cattaactat
     5641 gatgcctcaa agctaggagc gccagcactt agttttgata cgtatctgca ggacaatgtt
     5701 aaatatagag tgataattaa taatctttat ggcagtgagt ttcggataag agatattaat
     5761 gaggattttg ttgggatgga ttttacctgt gaattccgac ccgaaaaaat gacgaatttg
     5821 atcaatataa aaaataggtt ggttattcat tatttaagga ctatatacta tgaaaaaaat
     5881 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa
     5941 gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc
     6001 gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa
     6061 acgtttcggg tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg
     6121 tattgataag ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat
     6181 cgccgcgatg gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc
     6241 agcataagaa ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat
     6301 cggtttgctt cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta
     6361 gcgccgtacg gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta
     6421 ctggcaggcc catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac
     6481 cggcaacgcc tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt
     6541 ccagcgggtt aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac
     6601 cggcgcagcc gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt
     6661 caggaacgcc cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg
     6721 aacggctggt aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac
     6781 tcacgccatt aatgcccagc agaatgccat gcacagcaaa cataatcagc agaccgaggt
     6841 aggacgcgac aacgaaactt cccagtttga tgatgtcttg caagttagaa cctgcaacca
     6901 ctttggtcat tagagccaga acgccgtaag gggtcaactg catgacaagg cgaaccagtt
     6961 tcatcaccca gctttgtagg gtatcgatag cggttaagac gcgttcacct ttcggcgcat
     7021 catccttcag cagtttcaat gcagctacgc cgaggaatgc ggcaaaaatt accacgctga
     7081 taattgacgt cggattggct ccggtaagat cggcaaacgg gtttttcggg ataaaggaca
     7141 agaccagctg cggaacgctc agatcagaga ctttaccaac atagttactt tcaatggcgt
     7201 tcagacgtgc agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt
     7261 ggtgaccagc acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccgatggt
     7321 cagaaaactg attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat
     7381 agaggcgaag actaacggca taacgatcat ttgcagcagt tgaacattgc cgttaccaac
     7441 gatgttaaac cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg
     7501 cagggcaagg ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact
     7561 ccactgttta tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt
     7621 cgcaattaat ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag
     7681 cagtcatagt gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga
     7741 attgcttatt ccagaaaggt gatttttgcc tttttagtat tcaacctgat gcttaataag
     7801 ccgatgaaac tggatctgca aggtattgat catttgcgat gaccaacgaa cagcgctgtt
     7861 ctcgggtaat gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg
     7921 ttccagttgg ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg
     7981 ccagagcagg ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc
     8041 cagcaccatt ccttgtagcg catcagccgg aatgaaccag ccttccggaa ccttaaggta
     8101 aaaggttgcc agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa
     8161 agcgcgggcg atcggttttg atatccattt taatcgctgc ccaagaaacg actttgggtg
     8221 atcgatgtcc ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc
     8281 ctgtgccagc acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa
     8341 gagaaggtga ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata
     8401 ggatttttat acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat
     8461 taatccgtca gttgaatgca aattcagata aagaaccagg ggcagcgtga tcactgcccc
     8521 tggtcattaa ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtt
     8581 tgctaataca aaacgtggat cattttgcgc ttctggcgca ggaacgacga tggaacgcat
     8641 gcaggctgct ttagaagcga tcatgccatt taccgagtct tccagcgcca cgcaggtcag
     8701 tgggtcaacg cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta
     8761 aggcagtttt tcagccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa
     8821 cactttttcc agcatatgta gtggtgacgc ggaggccagt cccaccaata aaccttgttc
     8881 tttgcataac gcaacagctt cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag
     8941 tgaaatggca cgggcaataa cccgttctac tacttcctga cggcttggcc cattccacgg
     9001 ttgccgggcg tacaaagatc gaccaccata tcgatgcgta aacctaaggt gtccggcagc
     9061 tcgttacgac gggagatatc tacccccagg cttgccatca catccagttc ggctcgatcc
     9121 cataaaggtt ctgagtcgat aagtaatcca tccatatcaa aaattgcagc aagaatctga
     9181 cgcggggttg acattacaac ctctccttta gagcattatg tgtaaggtat taagccgtgc
     9241 ttatgattaa gcgcctatca tatcgccttt aaagaatggg cgaaaacaga tatctacagg
     9301 taaactaagt gaaacgacgc gtcttaatca aggagaactc atgacgtatc aacaagctgg
     9361 acgcattgct gttttgaaaa ggattttggg ttgggtgatt tttatacctg cattgatctc
     9421 tacattgatt tctttgctga aatttatgaa tacccggcag gaaaaccagg aaggcattaa
     9481 tgcggtcatg ctcgactttg ctcatgtcat gatcgatatg atgcaggcga atacgccttt
     9541 tttgaatctg ttctggtata actccccgac ccctaatttt aatggtggtg tgaacgttat
     9601 gttctgggtg attttcatcc tgatttttgt cggactggcg ttgcaggatt ccggtgcccg
     9661 gatgagtcgc caggcgcgct tcttgcggga aggtgttgaa gatcaactca ttctggaaaa
     9721 agccaaaggg gaagaggggc taacgcgtga acaaattgag tcccgtattg ttgttccgca
     9781 ccatactatt ttcctgcagt ttttctcact atatatcctg ccggttattt gtattgctgc
     9841 aggttatgtg ttcttttctc tgcttgggtt tatttaatca aaaggccgca cggagcggcc
     9901 attatcctca acccatatca tgctgctaat aatctatcca atgactgctg agcaataacc
     9961 aaatgctgac cgccaaataa tcttgcacga tttaacagcg tgtagagttg gtaaaccggt
    10021 tgacgttcaa ggaaatcggc aggtagcggg gatactgact gatagccgtc atagatttgt
    10081 ggcggctgtt cagtatgcag cggtaacatc gccaggtcgc actctcggtc accccagtag
    10141 caggcagggt cgaaaatgta cgggccatcc ggacccagcg cacagttgcc ggaccataaa
    10201 tcgccgtgca acagagaagg ctgcggttga tgcgaggcca gacgctgctg aatatgctcg
    10261 acgatggcgt cgatattgcc gaaagcgatc cctttctctg ctgccagttc caactgccag
    10321 ccaatccgtt gttcagcaaa aaacgttgac cagcgacgtt gccaggtgtt gggctgtgga
    10381 gttgtggaga gcgagttatc aaaatcgagg ccaaattgtg gttggtcact ccattgatgt
    10441 aaacgcgcaa tttgctgacc aagaataaat gcgctatgcg catccagcgg acgaggtggg
    10501 agataatcca tcaccagaaa actgtagtca cggtcagcgc ctactgccca aaccttaggc
    10561 acggtgacgg ttttactacg cgacagtaac tccagttggt cggcttcggc ggtaaaactg
    10621 ggaagcagtt ccctttcatc acatttgacg aaaaagtcat gtcctgcata gcgcaaatgc
    10681 catgcggcat ggacttctcc gccaggcagt tcattacgca gttcgatttc gccttcacct
    10741 aactgctcgc tcaaaagacg actgattgcc tgccacatga tgtctctccc atgttgtctg
    10801 ccagatcata aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag
    10861 actgattatt gcccaaaaac catttatttt ctgttgctat tccattcctc ccaggtcgtg
    10921 atggtgattt caggatcttt gccggttgca ctttgggtaa gccccgaaac cagttctcta
    10981 atttcttctt cactttgggt actgataagt ccaaaagtgt tagtccccag ctcatggata
    11041 tttccctcat catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta attattcaac
    11101 tcgttaattt cagtcaatgt atcctcatgc aacatgacgg ttatgacata acggacaaga
    11161 tcgccactgg ccatagttca cccctttgtt atcacatgac ttttgagcat agtcggagaa
    11221 acgcgttgcc gacaggttgg tggtgattcc cccaatgctg ggggaatgtt tttgttagcg
    11281 ggaaaggtaa gcgtaaattt tttgcgtatc gtcatgggag cacagacgtg ttccctgatt
    11341 gagtgtggct gcactccccg cagctacgcc aaaacggacc atctcttcaa gagaggcatt
    11401 ttctgccagt ttcagtgtca tcgcgccgac catgctgtca ccagcgccaa cggtactctg
    11461 gcttttcacc ggtggtggca ccacctgaat gcagttttca ctatcaacac ccagcgctcc
    11521 ttgtggacca agggaaacga caacccgttt ggccttgccg ctattaacga tttcctgcgc
    11581 ggctttgcgg acatcgtctg gctgggtgag ttcgcgactc accagcgcac tgagttcttt
    11641 ttggttaggc ttaaccaact cgatgttacc aatcgccagt gctgcactta acgcttcgcc
    11701 agaactgtcg atgatgcagc ggatcccttg gttttgcgca gcggaaatca gttgggttaa
    11761 tttttccagc ttcacacctg gcggcaggct tccgcttatg accaggatgg ccccggattc
    11821 aatttccaga acttgctctt caagctggcg aaactcatct tcatttaatg ccgcgcctgg
    11881 cataacaaaa cgatactgtt caccgcttgc ttccacatgt acgtgtaaat tctgccgggt
    11941 ccagtctttg gcttctacag tagcgacggg gacattttca tccgccaaca gtgaaaccag
    12001 gtgttcgccg gtcgcgccac ccgccgggaa gatcgctgtg gcactgcctc caagatgggc
    12061 aatggcgcgg gcgacgttga tgccgccgcc cccgggttcg aacaccggtg cggtacagcg
    12121 cagttttcct tcgggataaa tttgcggggt aattgttgcg ctatcgagag agggcgcaag
    12181 tgtcaacgta tagatacgta ccatcatttc ctcctatagg ctgatttcag tctggcaccg
    12241 aatcagcgga aagtgaagaa attaacaata tgatttatta gagctttatt taaaattttg
    12301 cagataaata tatataaata aaaatctgtt ttcgccacat gcttattcaa aaaatgaaat
    12361 aagaatacat tgctaagtta ttcgcgcctt tttataattc gttacctttc cagggacgct
    12421 aatctttgat ccttaagaaa gtttccggga ccgtgatggt ctcttttttg ttgtacggac
    12481 tcttgataaa tgaagctttt gaagacagtt cctgccatcg ttatgttggc ggggggcatg
    12541 tttgcgtcac tgaatgcagc tgccgatgat tccgttttta ctgtcatgga cgatcctgcc
    12601 agcgctaaaa aacctttcga aggtaatctg aacgcaggat atcttgcaca atcaggcaat
    12661 acgaaaagct cctcacttac ggctgacacc accatgacct ggtatggaca aaccactgcc
    12721 tggtcgctgt gggggaacgc cagtaatact tcttctaatg atgagcgttc ttcagaaaaa
    12781 tatgcggcgg gcgggcgtag ccgctttaat ttaactgatt atgactattt atttggtcag
    12841 gcaagctggc tgacagaccg ttataacggc tatcgagagc gcgatgtgtt gaccgcgggt
    12901 tatggtcgtc agtttttgaa tggcccggta cacagtttcc gctttgaatt cggtccaggt
    12961 gtgcgttacg acaaatatac cgacaatgcc agtgaaaccc agccgctggg ctatgcctcc
    13021 ggggcttatg catggcagtt gactgacaac gctaaattta cgcagggtgt ttcggtcttt
    13081 ggcgcggaag acacaacgct gaactccgaa agcgcattaa atgttgctat taatgaacat
    13141 tttggactga aggtggctta caacgtcaca tggaactctg aacctccaga gtctgcaccg
    13201 gaacataccg accgtcggac aaccttatcg ctgggctaca gcatgtaaaa ttatctgccg
    13261 gaccgtgtta tgcggtctgg ctgtcttcgt ttgtcactgc tcagccccaa atcattgctg
    13321 cccaacgtag caacaacata gcgccacaga ttgccagtag caccaccacc atcttccagt
    13381 gttttttatc aaagcgttta gtcggcatac cagtgtcctg catatctctt gtgcgtgata
    13441 acggagtgta acaaaaggga caggcatcaa gggatttttt gttattacag ccgtcgggtg
    13501 gcgttgtgca catggtcact gtttctgcgt tttatgaaaa aaataaaatt atgtatcgat
    13561 atatatcgta aatagctggt tataaatacg tctttatttc atcagcacta aaactcaggc
    13621 tttggctgat gacatttata gcagcataat atgtttctac agttttataa tgcttatgat
    13681 gaatggctat atgaacagct gacatgttgt catgattttt agcggccaat agtgtaaata
    13741 actgatgttg actaaaagaa tgtttttttg caaaagcacc cagcgtcgat atataagata
    13801 acacgacgtc ttcattaccc ttgctcatgg cgatgtataa tgcagggggt ccctgtgctg
    13861 tagctccttt taataaatcc atgatgttag ctctgctcaa cttgtattta aaggcgatgc
    13921 cattaatttt agagaggaac cgtgtgacac acaaagggtg attattttcc attgcggcgt
    13981 ataatcctgg ctcaccatca tg
//
LOCUS       DAJTRQ010000103        13954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000103 DAJTRQ010000000
VERSION     DAJTRQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..493)
                     /locus_tag="OO332_003064"
     CDS             complement(<1..493)
                     /locus_tag="OO332_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5943463.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO332_003065"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO332_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5943464.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="OO332_003066"
     CDS             complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="OO332_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5943465.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2440..3306
                     /gene="folD"
                     /locus_tag="OO332_003067"
     CDS             2440..3306
                     /gene="folD"
                     /locus_tag="OO332_003067"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5943466.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3308..3520
                     /gene="ybcJ"
                     /locus_tag="OO332_003068"
     CDS             3308..3520
                     /gene="ybcJ"
                     /locus_tag="OO332_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5943467.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3628..4149
                     /locus_tag="OO332_003069"
     CDS             3628..4149
                     /locus_tag="OO332_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5943468.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="OO332_003070"
     CDS             complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="OO332_003070"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5943469.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5744..6238
                     /gene="ppiB"
                     /locus_tag="OO332_003071"
     CDS             5744..6238
                     /gene="ppiB"
                     /locus_tag="OO332_003071"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5943470.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6241..6963
                     /gene="lpxH"
                     /locus_tag="OO332_003072"
     CDS             6241..6963
                     /gene="lpxH"
                     /locus_tag="OO332_003072"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5943471.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7081..7590
                     /gene="purE"
                     /locus_tag="OO332_003073"
     CDS             7081..7590
                     /gene="purE"
                     /locus_tag="OO332_003073"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5943472.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7587..8654
                     /gene="purK"
                     /locus_tag="OO332_003074"
     CDS             7587..8654
                     /gene="purK"
                     /locus_tag="OO332_003074"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5943473.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8791..9684)
                     /locus_tag="OO332_003075"
     CDS             complement(8791..9684)
                     /locus_tag="OO332_003075"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5943474.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9681..10328)
                     /locus_tag="OO332_003076"
                     /pseudo
     CDS             complement(9681..10328)
                     /locus_tag="OO332_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10331..11563
                     /gene="allC"
                     /locus_tag="OO332_003077"
     CDS             10331..11563
                     /gene="allC"
                     /locus_tag="OO332_003077"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5943475.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11574..12359
                     /gene="allE"
                     /locus_tag="OO332_003078"
     CDS             11574..12359
                     /gene="allE"
                     /locus_tag="OO332_003078"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5943476.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12587..13732)
                     /gene="glxK"
                     /locus_tag="OO332_003079"
     CDS             complement(12587..13732)
                     /gene="glxK"
                     /locus_tag="OO332_003079"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCR5943477.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13754..>13954)
                     /locus_tag="OO332_003080"
     CDS             complement(13754..>13954)
                     /locus_tag="OO332_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR5943478.1"
                     /translation="QQITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSN
                     GLLVGILLAVLMDNLIPWERIE"
BASE COUNT         3386 a         3680 c         3497 g         3391 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga taaacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa gattgctttg
     1261 gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac aaagtgaacg
     1321 tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta ctttgttata
     1381 agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca taataaaagt
     1441 cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga
     1501 aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc tattcccatc
     1561 aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg ttgctgttgt
     1621 tgtatttgta ctggatgcaa tggccaataa attatcattg attgcatcag cctgaccaga
     1681 aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact gaatggtgaa
     1741 tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat attgaccgag
     1801 tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca ccagttcgcc
     1861 ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag cggcgaatag
     1921 tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt caatctcatt
     1981 tatcgactcc acatccgtat ataaccgatt actttattta agacactgat agtagtaaaa
     2041 ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc taaaaaatag
     2101 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
     2161 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
     2221 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg tacgaaaaat
     2281 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
     2341 cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aatagacgca
     2401 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
     2461 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggctcgtatt
     2521 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
     2581 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
     2641 tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat
     2701 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
     2761 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac
     2821 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
     2881 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
     2941 tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg caccaccacc
     3001 gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctgttgatc
     3061 gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaagaagg cgcaattgtg
     3121 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     3181 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     3241 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt
     3301 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     3361 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     3421 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     3481 agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     3541 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     3601 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc
     3661 ctttgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     3721 atcatcctag cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg cgttgcttac
     3781 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     3841 ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg ctgctggctg
     3901 tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac tggcggtaag
     3961 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     4021 aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca ggtgattatt
     4081 tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc
     4141 agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt acgacgccag
     4201 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgtgcc
     4261 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     4321 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     4381 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     4441 gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagcacg
     4501 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     4561 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagaggcgc
     4621 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag
     4681 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     4741 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccattccg
     4801 gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg cgcgatttcg
     4861 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     4921 agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc cgcgccccac
     4981 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     5041 ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc gagatcctga
     5101 cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     5161 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     5221 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     5281 tgcatttcgg caatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     5341 cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     5401 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata
     5461 tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata
     5521 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     5581 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     5641 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     5701 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     5761 caatcacggc gatattgtca tcaaaacttt tgacaataaa gcacctgaaa cagttaaaaa
     5821 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     5881 cggctttatg attcagggcg gcggttttga acctggcatg aaacaaaaag ccaccaaaga
     5941 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     6001 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     6061 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     6121 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtaacggtat
     6181 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     6241 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     6301 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     6361 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     6421 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     6481 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     6541 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     6601 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     6661 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc
     6721 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgctgtggtc
     6781 agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg ggcataccca tcgcccggcg
     6841 gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg tgcctggcat
     6901 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     6961 taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     7021 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     7081 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     7141 gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca ccacgttgaa
     7201 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     7261 aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt
     7321 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     7381 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     7441 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     7501 gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga cgaagtgctg
     7561 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     7621 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     7681 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     7741 gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     7801 atgtgttccc gattattgct gaccgtctga ctcagaaaca gcttttcgat aagctccacc
     7861 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     7921 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     7981 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt gaatgtattg
     8041 tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc ggctttgatg
     8101 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     8161 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     8221 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     8281 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     8341 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     8401 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgtgaatt
     8461 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     8521 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     8581 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     8641 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     8701 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8761 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     8821 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     8881 gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     8941 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9001 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9061 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     9121 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9181 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccagc gcaaatattt
     9241 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9301 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     9361 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9421 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     9481 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     9541 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     9601 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     9661 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     9721 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     9781 gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga ggcgaaatat
     9841 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     9901 tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata ataccaggcc
     9961 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10021 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10081 tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc gaaaagtccg
    10141 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga aggcggcgta
    10201 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
    10261 ttagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10321 gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct ggctttcctc
    10381 ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg aatggctgga
    10441 aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac gtttcgatga
    10501 agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag tggttctgag
    10561 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    10621 ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt tacgtacggt
    10681 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    10741 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    10801 aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgcctaacg ccccactaac
    10861 tccgcgtcag gatataaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    10921 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gctatacggt
    10981 gacgctgaac ggcgagtcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    11041 ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga
    11101 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    11161 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    11221 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    11281 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    11341 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    11401 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc ccagcatcaa
    11461 cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg aaggggtcaa
    11521 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    11581 atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg attgttaaac
    11641 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    11701 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    11761 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    11821 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    11881 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    11941 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    12001 caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat gaaggtatgg
    12061 atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg aacatgcata
    12121 tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    12181 gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg atcccggtga
    12241 aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat ggcgtagggc
    12301 gtggtgaagc gttcagctat atttactcga aagattgcaa ccgcgacgta gagatttaag
    12361 tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt tataccgtgg
    12421 ctttttttgt aacattgcct gatgcgcttc gcttatcagg cctacatgac ctctgcaata
    12481 tatagaattt gcgcgatttt caggaaggat atagtactca tgccgcatcc ggcatgagta
    12541 ctgcgctctt atacgaaata aatcgcatcc gctttaaagg caagagtcag tttttaattc
    12601 cctgacctat tttaatggcg caggcaatat ttcgcgcgct gttgaagaga ttggtttcac
    12661 cgctggcgag cacttcggct aaaggtgcca gacgaggcaa aatgctgaat agcgcgtcaa
    12721 tgccgtactg gtgcaccact tccacgccat cacccaatac accagcaatc ccaatcaccg
    12781 gtacattaaa ctgcttcgcc accgacgcaa cgcccagcgg cgctttaccg cctgccgttt
    12841 gcgagtcaat gcgcccttcc ccggtgatca ccagtgctgc cccctgcacc gcctgcgcaa
    12901 gattgaccgc attcaacaca atctcaatgc ccggtttaat atccgcattg agaaataccg
    12961 ccgccgcaat ccccataccg cccgcagcgc cgccgcccgc catctgacag acattaattt
    13021 cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc ctgttccagc tcttcaacca
    13081 tctccggcgt tgcccctttt tgcgggccaa ataccgccgc cgcgccgcgt gcccctacca
    13141 gcgggttatc aacgtcacat gccacttcaa tatggctgtt cgccagacgc ggatcgcatt
    13201 cctccatctc aatgcttgcc acgcgcgcta aattgccgcc gttagccgcc agcacctggc
    13261 cgtcggcatc aaggaaacgc acaccaagcg cctgcgccat gcccataccg ccgtcgaccg
    13321 tcgcactgcc gccaatgccg agaataatat ggcgaatgcc gttatccagc gcatggcgaa
    13381 ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt acgcttttca ggcgcgacca
    13441 gcatcaggcc acttgctgcc gccatctcaa tcaccgctgt tttcccgtct ccggtaaggc
    13501 cataaaaagc attcactttt tcgcccatcg gcccgcagac ttcaagcgtc acgatgttgc
    13561 cacccgtcgc ggcgaccatc gcatccaccg tcccttcgcc gccatccgct atcggcaaac
    13621 agatatagtg cgcatcggga aagagggtcg aaaacccggc tttaattgcc tgacaacatt
    13681 tttctgcact taagctctcc ttaaaagagt ctggcgcaat gacaatcttc attatcaatc
    13741 ctttttcaac aaattattcg atgcgttccc acggaataag attatccata agaacagcca
    13801 gtaaaattcc gaccagtaag ccgttactga gcagaggacg aagcgttaat ggaaggtctt
    13861 gcaaatacac aggcggtaat gccattaaaa atatgccgac aaataacggc aatgcgagtc
    13921 gataaatatt gcgctcggta aacgttattt gctg
//
LOCUS       DAJTRQ010000104        13789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000104 DAJTRQ010000000
VERSION     DAJTRQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..139)
                     /gene="csgB"
                     /locus_tag="OO332_003081"
     CDS             complement(<1..139)
                     /gene="csgB"
                     /locus_tag="OO332_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5943479.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QA"
     gene            893..1543
                     /gene="csgD"
                     /locus_tag="OO332_003082"
     CDS             893..1543
                     /gene="csgD"
                     /locus_tag="OO332_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5943480.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1548..1937
                     /gene="csgE"
                     /locus_tag="OO332_003083"
     CDS             1548..1937
                     /gene="csgE"
                     /locus_tag="OO332_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5943481.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1962..2378
                     /gene="csgF"
                     /locus_tag="OO332_003084"
     CDS             1962..2378
                     /gene="csgF"
                     /locus_tag="OO332_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5943482.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2405..3180
                     /locus_tag="OO332_003085"
                     /pseudo
     CDS             2405..3180
                     /locus_tag="OO332_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3244..3735)
                     /locus_tag="OO332_003086"
     CDS             complement(3244..3735)
                     /locus_tag="OO332_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5943483.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3837..4391)
                     /gene="ycdY"
                     /locus_tag="OO332_003087"
     CDS             complement(3837..4391)
                     /gene="ycdY"
                     /locus_tag="OO332_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5943484.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4415..5152)
                     /gene="ycdX"
                     /locus_tag="OO332_003088"
     CDS             complement(4415..5152)
                     /gene="ycdX"
                     /locus_tag="OO332_003088"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5943485.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5207..6145)
                     /gene="ghrA"
                     /locus_tag="OO332_003089"
                     /pseudo
     CDS             complement(5207..6145)
                     /gene="ghrA"
                     /locus_tag="OO332_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6379..6466
                     /locus_tag="OO332_003090"
     tRNA            6379..6466
                     /locus_tag="OO332_003090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6413..6415,aa:Ser,seq:gga)
     gene            complement(6616..6897)
                     /locus_tag="OO332_003091"
                     /pseudo
     CDS             complement(6616..6897)
                     /locus_tag="OO332_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7179..8183)
                     /locus_tag="OO332_003092"
     CDS             complement(7179..8183)
                     /locus_tag="OO332_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5943486.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8255..8815)
                     /locus_tag="OO332_003093"
                     /pseudo
     CDS             complement(8255..8815)
                     /locus_tag="OO332_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(8900..9097)
                     /locus_tag="OO332_003094"
     CDS             complement(8900..9097)
                     /locus_tag="OO332_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943487.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9109..9600)
                     /locus_tag="OO332_003095"
     CDS             complement(9109..9600)
                     /locus_tag="OO332_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943488.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9597..9971)
                     /locus_tag="OO332_003096"
     CDS             complement(9597..9971)
                     /locus_tag="OO332_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5943489.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10018..10392)
                     /locus_tag="OO332_003097"
     CDS             complement(10018..10392)
                     /locus_tag="OO332_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5943490.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10555..10776)
                     /locus_tag="OO332_003098"
     CDS             complement(10555..10776)
                     /locus_tag="OO332_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5943491.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10839..11315)
                     /locus_tag="OO332_003099"
     CDS             complement(10839..11315)
                     /locus_tag="OO332_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5943492.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11331..11804)
                     /locus_tag="OO332_003100"
     CDS             complement(11331..11804)
                     /locus_tag="OO332_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5943493.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12146..12967)
                     /locus_tag="OO332_003101"
     CDS             complement(12146..12967)
                     /locus_tag="OO332_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5943494.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13067..13240)
                     /locus_tag="OO332_003102"
     CDS             complement(13067..13240)
                     /locus_tag="OO332_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5943495.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13360..>13789)
                     /locus_tag="OO332_003103"
     CDS             complement(13360..>13789)
                     /locus_tag="OO332_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943496.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3523 a         3372 c         3321 g         3573 t
ORIGIN      
        1 ctgcctgatt aaatgaagac ttactcaatt catttaccgc gaagttatat tctgaattag
       61 ctaaatcata acctgctgcg gctgcaatcc caggcgcacc cagtattgtt aacatcataa
      121 ataacaattt gtttttcatg ttgtcaccct ggacctggtc gtacatttaa gaaattaaat
      181 catttcaact tggttgttaa cgcaacctgt attttgttaa cgctgcgtta cgatggaaag
      241 tatgtctgcg gaaatatttt taataactca cccgcgcgtt gtatttttct ttttttgttc
      301 ataccaaagt attaaaaccc gcgtaaatac gctgttatct acgcaaaaaa tattttgttt
      361 tctcttaaat ctccgttttc cgctatcaaa aatcaccaga cagtcattct tcttgcccgt
      421 cgctgattgc tgcgatatgt cttgcgcaca agccgtgaca aacctcgtct ttttgcagta
      481 aaaaaattgt ccacggaggt gtggagaaaa aacaaaaacg ttttacatga cgaaaggact
      541 acaccgaaat attttttata tgtattatta gtaagttagc accatttgta tgatttttta
      601 aaattgtgca ataaaaacca aatgtacaac ttttctatca tttctaaact taataaaacc
      661 ttaatgttaa cattttaata taatgagtta cattttgtta catgtttaac acttgattta
      721 agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat tttacccatt
      781 tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc tggtgcttct
      841 attttagagg cagctgtcag gtgtgcgatc aataaaaaag cggggtttca tcatgtttaa
      901 tgaagtccat agtattcatg gtcatacatt attgttgatc actaaacctt ctttgcaggc
      961 gacagctctc ttgcagcacc ttaaacaatc gctagcaatt acaggaaaat tacataatat
     1021 tcaacgttct ctggacgata tctcttcagg ctctattatt cttctggata tgatggaagc
     1081 ggataaaaag cttatccatt attggcagga tactttgagc aggaaaaaca acaatatcaa
     1141 aatattgttg ctaaatacgc ctgaagatta cccgtaccgc gacattgaaa actggcctca
     1201 tatcaacggc gttttttatg ccatggagga tcaagaacgt gttgtcaatg ggttgcaagg
     1261 cgtcctgcgt ggcgaatgct actttacgca aaagcttgcc agttacctga ttacacattc
     1321 tggtaactat cgttataaca gcacggaatc agccctcctt actcatcggg aaaaagagat
     1381 cctgaataag ctgcgtatcg gcgcgtctaa taacgagatc gctcgttcgt tgttcatcag
     1441 cgaaaatacg gttaaaacgc atctttataa tcttttcaag aagatagccg taaaaaaccg
     1501 gacacaagcg gtttcctggg caaacgataa cctcaggcga taaagccatg aaacgttatt
     1561 tacgctggat tgtggcggca gaatttctgt tcgccgcagg gaatcttcac gccgttgagg
     1621 tagaagtccc gggattgtta actgaccata ctgtttcatc tattggccat gatttttacc
     1681 gagcctttag tgataaatgg gaaagtgact atacgggtaa cttaacgatt aatgaaaggc
     1741 ccagtgcacg atggggaagc tggatcacaa taacggtcaa tcaggatgtt attttccaga
     1801 cttttttatt cccgttgaaa agagacttcg agaaaactgt cgtctttgca ctgattcaaa
     1861 ctgaagaagc actaaatcgt cgccagataa atcaggcgtt attaagtacg ggcgatttgg
     1921 cgcatgatga attctaaata aaaaattgtt cggaggctgc aatgcgtgtc aaacatgcag
     1981 tagttctact catgcttatt tcgccattaa gttgggctgg aaccatgact ttccagttcc
     2041 gtaatccaaa ctttggtggt aacccaaata atggcgcttt tttattaaat agcgctcagg
     2101 cccaaaactc ttataaagat ccgagctata acgatgactt tggtattgaa acaccctcag
     2161 cgttagataa ctttactcag gccatccagt cacaaatttt aggtgggcta ctgtcgaata
     2221 ttaataccgg taaaccgggc cgcatggtga ccaacgatta tattgttgat attgctaacc
     2281 gcgatggtca attgcagttg aacgtgacag atcgtaaaac cggacaaacc tcgaccatcc
     2341 aggtttcggt tttacaaaat aactcaaccg atttttaagc cccagcttca taaggaaaat
     2401 aatcatgcag cgcttatttc ttttggttgc cgtcatgtta ctgagcggat gcttaaccgc
     2461 cccgcctaaa gaagccgcca gaccgacatt aatgcctcgt gctcagagct acaaagattt
     2521 gacccatctg ccagcgccga cgggtaagat ctttgtttcg gtatacaaca ttcaggacga
     2581 aaccgggcaa tttaaaccct acccggcaag taacttctcc actgctgttc cgcaaagcgc
     2641 cagggcttac aaaacctgct taacgagcgc aaggttattc gtgcggcaca agaaaacggc
     2701 acggttgcca ttaataaccg aatcccgctg caatctttaa cggcggcaaa tattatggtt
     2761 gaaggttcga ttattggtta tgaaagcaac gttaaatcgg gcggtgttgg ggcaagatat
     2821 tttgggatcg gtgcagacac gcaataccag ctcgatcaga ttgccgtgaa cctgcgtgtc
     2881 gtcaatgtga gtaccggtga gatcctctcc tcggtgaaca ccagtaagac gatactttcc
     2941 tatgaagttc aggccggggt tttccgcttt attgactacc agcgcttgct ggaaggggaa
     3001 gtgggttaca cctcaaacga acccgttatg ctgtgtctga tgtcggctat cgaaacaggg
     3061 gtcattttcc tgattaatga tggtatcgac cgtggtctgt gggatttgca aaataaagca
     3121 gaacggcaga atgacattct ggtgaaatac cgccatatgt cggttccacc ggaatcctga
     3181 cagataaaaa tgccggaatt aataatccgg catttactgt ttaagcgaaa gcgtgagtgc
     3241 ccctcacgct ttatttttga tttcctgctc acggtgagcc gtctttgcac tacgcgccgc
     3301 cagccacagg ccgctgtttt tcattgcgta accaaatacc agcccaagcg ccagcgaagg
     3361 taacaccagt ttccagtcac cctgcccggc aaacgtcgcg catgcgccta taaacgtccc
     3421 cggaacaaat gaaagcagca gctgtttggc ctgaatacac atcagaaacg cgacaatacc
     3481 ggttatgaca taaccgagaa tttccagatg tggtgccagt gcactaccgt aaataatgac
     3541 catcgcccac accacgccac taagcagggt tgcagcggag atcgccagcc ctttcagccc
     3601 accttgcggg caagcaaagt aggccgtaca gcccaggaag cccgcccagc ttagtaaacc
     3661 cagggaaaca gccacccatc cccagatacc ggagagaatg cctgttgtga ttgcaataga
     3721 gagaagtatg ttcatgccgc gcatgatagc agaaaacgcg gccatgaatg agatcgagca
     3781 cacattttat gcaacaacaa attatctgtt gcagttatga gtgacgatga tcacatttac
     3841 tcttcagaat cttcttccag ctcgtcccac attgcactaa tggcatcacg ggttagcggt
     3901 gccatggtgc gccagaaagg cgtggttgca tgggcctcca ctttgccgag gaacgcacca
     3961 caccagggta acagatactc actgaacagt gtttccagtg cttcgctctc atcttccgtt
     4021 gactgatctt ccagccagga agccgcaagc agcaatgtgc cgatgtgatc ggctggcgta
     4081 tccgccagtg gcatccctcg ttcggaaaga aaagcgcgca cttccgcttc cgttgcgtcc
     4141 tcaacccatg cgctacgata tggcggcacg gcacattcat cgccgataaa caacgcattg
     4201 taatcggcag agacttgcgt catatcacag cttttctgta aacgcgtcag taactcatcc
     4261 tgctccagtg gccagttcgc agccagtttc ccctcacgaa tcagagtaaa cagcggcacc
     4321 agtaaaggat cttgcggttg gcggtaatag agcgaaccca gcacacgaca gaggatagaa
     4381 aactcgttca ttaatatatt tccattacta gtaattaaag gtctgcaaat tccgcaatcg
     4441 gtgccatacc gcgagattca aggaagttca gtaagcggcg cggagaaaca ttcaaaatgc
     4501 gctctggcgg aaaatctacc gcgtcgagga ttttaagaca ctcttcaaat tcccccatgg
     4561 taaacgcggt gtgagaatcc gagcctaatg ccacccaacc tccagcatca cgtaccgctg
     4621 cagctaccgc acgacagttg tcttcactgc ccttacgtga gtgtaaaaat gaggaattat
     4681 tgatttccag cgccacctga tgtttcgcgg ctgcttcagc aacggcttta acatctattt
     4741 catatttcgg atttccggga tggcttatta tatgcacatt gccgctggcg atagtggcga
     4801 tcatcgcttg tgtattggtc gctttgtcat gtggcgcaaa aaccggctca tgaaaaccgg
     4861 caataatcag atccagcgag tcaaacattt taccgctgca gtcaatttca ccatcaacat
     4921 ttttaatgtt agcttcaatg ccgcgcagga tccctacccc atcaaccact cgcggccaga
     4981 tacgcatgtt aatgaagtgc cagtgatgcg gcgcatcttc catatccggg ccatgatcgg
     5041 tgatcgcaaa aagtttaatg cccttttgtt tggcctgggc aatgtaatca ctcaatgtgc
     5101 tatatgcatg tgtgctggca acggtatgca tatgaaggtc gacgggatac ataactctct
     5161 cctgtattca attcccgcca aggatagcag gaatcctgat gctttattag tagccgcgtg
     5221 cgcggtcgac ttgcccgcag accctctccc ctttttcgag ctgggcaata gtgcgagaaa
     5281 tgtactccac agcttcagcg ggacgggtaa tcgcggcgac atgtggtgtt atcgtcacgc
     5341 gtggatgttg ccagagcgga ctttcaggcg gtaagggttc acgattaaaa acatccagca
     5401 ttgcgccttt aactttcccg ctatccagcg ccgcgagcag gtcatcttcc acaacatgaa
     5461 caccacgcgc caggttgagg agatacgcgc catcaggtaa tttttcgagt aattgttgat
     5521 taataatgcc gacggtttca ggggtattcg gtaacaaatt aatcaatacc cgacattggc
     5581 tcagaaatgc agacagttct tcccgtccgg caaagctttg cacgccaggc cacgatttac
     5641 gggttcgact ccagcaacgc agcggaaagc gctaggtttg cagactctga gcaactttac
     5701 agcccagtac gcctgcgccc aaaatgccga tggtaaaatc ttcccgatga tattcaggca
     5761 gcggttgcca atgcgaacta ttttgctgga tgcgataatc atcaaaacgt cgaaaccaat
     5821 gcagcacctg actgacagca tattcctgca tttgctcgcc cataccggta tcttccaggc
     5881 gaaaaagtgg aacagaaggg ttcagcattt cagggtgtgc ctgtagcttg ctcaaaatag
     5941 aatcaacacc ggccccgagt gcgaacaccg ctttaagatc gcgccctgcc agcatttcaa
     6001 caggaggatg ccagactaaa acataatcag cagagtcatt atctccgctt ttccatgccc
     6061 tgactcttgc ctgaggaata gctttgcgca gtgcctcaat ccaccattgg gtatcgaacg
     6121 ttgggtgata aaagatgata tccatactga ctcccgaaaa gcgttgtgcg aaatttattc
     6181 gcacttatcg ttatgatcta caaaggccac cagcataaca aatccgtggt cggtggcaaa
     6241 aaaagcagat ttcgcttatt aaaaccacac attgattgaa gtttgaataa acgcgcgatt
     6301 ctttcaaaaa gtttgttgac ctcaggtcat gatttcccta aattagcgcc cgttccagca
     6361 agacaggaac gacaatttgg tgaggtgtcc gagtggctga aggagcacgc ctggaaagtg
     6421 tgtatacggc aacgtatcgg gggttcgaat ccccccctca ccgccagatt ataagtgcat
     6481 gttattgttt aacatgcgct tttttatttt tccttccaga cagtatgcca tcgggtatgc
     6541 catctgaaac cggtttatat gccccgcgat catatcttat cggacaattt ccgatctttg
     6601 agcatactgg caaagctata ctgccagcat atcaggatag tgttttgcga taatatcatt
     6661 cagtctgtca accagttctg gttttctgaa cgtgatatgc gcggagccct tcttaaagta
     6721 tctgatgctg aacatctcat cttcatagct ctctttaccc tgaacagact gaatgtgatc
     6781 atccagacga agggtgatgt ttccccggtt gtcggggatt ggtttaccgc tgaacaggtg
     6841 caacattctt tccagatcgg cgagtcggtc agcctgttgt ccggtgataa gatgaaatct
     6901 atggtcaccc ccgtttttgc aacactgatt tttgattaat ggttggcttg cttaaatcta
     6961 tccggcgtct ctgtgggcaa ggatgcccgc gccccgatga gttccgcacc tgataatcct
     7021 aaaaatcccc tcggcttcag tgagccattt catctgttag gtttttaatc aggtcgattt
     7081 gccgttcatg tcatcaataa tcagtcttcg caaaaccaga aaggtaactt gtatcttatt
     7141 gtttaaaata aactaattga gttatatcag catctatatc aggcctgata ttcttttttc
     7201 tgagtagtga ttgcccaggc aattcgagcc agtttatttg ccagagcgca ggtaaccaca
     7261 ttcgaatgct tcttgttgag ttgctcccga acccactcag caagttttcc atgcttgcgt
     7321 tcaagtcgca ttatgaatga gcgggcacac tgaaccagta accggcgtaa atttttatca
     7381 cctcgcttac tgatacccaa cagagtcgat tttcctccag tgctatactg ccggggaaca
     7441 agtcctgtgg aagctgcaaa atccctgctg catgagaact gtttcccgtc accaagttgc
     7501 gacgatagta agctggcagt aatcggacca acaccaggaa ttgtcataat gcgctgagcg
     7561 gtatcatcag cgttgattga ctgacttaat tctgattcca gctctgctat ttgctcaaca
     7621 aggtaaaagt agtgggtatg caacctcacc aaaaggcggc tcagatattc ggggatttcg
     7681 tgttcagcca acacgagaga caaccgtttt attacagcat cacctatcgg caggctgatt
     7741 ccaaattcca gcaagaaacc gtggatctgg ttggttgttt tcaccttatc tctgatgagc
     7801 gattctctga cccggtgtaa cgccctcatg gcctgctggg tctcagttct tggctggaca
     7861 aaacgcattg atggtcgtga agccgcttcg catatggctt ctgcatcaac aaaatcattc
     7921 ttattacttt tgacaaacgg gcgaacgaac tgtggagaaa tcagcttagt ttcatgccca
     7981 aaatcagaga tacagcgagc cataaagtgt gcacccgcac aggcttccat tacaacaact
     8041 gagggcggac atgtcgccag aaactgcatc agtttagtac gggtaaattt tttacgcaga
     8101 agagccttac cagacttgtc ttgacaatga atgtggaagg agtgctttcc gagatcgata
     8161 ccaataagtg tgatgttctg catgatggtc tctccgttaa agggatttac cttaacagcg
     8221 tactgctgtt aaggtggggg tgaccatatc attatcccca ccggtcccat ctcaccagat
     8281 tgttgacgat aattttactg ccaaatttac aggggcaatt ggttttgtaa ttccagttca
     8341 gccctctgaa gacgttgatc acccctcgct caaacacctc atccttattc tggtgcagtt
     8401 gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatcg tactccagtg
     8461 accggtacca tgtatcacgg gcctgcgtat ccattaatga cagcatcccg gaacgttgca
     8521 tcaggtcacg ccagatttcg cgatcaagat tccgggtaat ggctttcatt gctgtttcgg
     8581 gtttctccat cagccagcag ccgcagcgaa aatcctgttt catggcccag tccggggcag
     8641 ttttacctcc gatgctttcc gtcagcaccg agatttcagc cagttgtcgg ataagtgtgt
     8701 cgatttgcaa cagtgtactc tttcgtacag ccagaatgtg gcgaatgctg gttgagcaga
     8761 taacgtccgt atgcccgttg accacttccg gacgggagat tgtgatatct gacataatcg
     8821 atattccttt ttgcagataa atgaaaacgc cagccctgtg caggctggcg tcagaggaat
     8881 gaagtctggt gagtgagctt caccggttct ggtgcagaag ctgcagatga cgcagggtac
     8941 ggacatcctc ccgggcctgt tcgatacagg gcaacagtgc tgctgaatcc gttttatcct
     9001 catcgttgtc aaagataatt cccgatccgc agtcgatatt gtcctgcagc cacgcaatca
     9061 gaatatccag cgctgtttcc gtggttaatg atttcatgtt gttcctggtt accagtcgaa
     9121 agtgggtaaa cccggcaggc attcagcgct ggcatccaga tgaatgagac tgacgccata
     9181 acgccggatg agtgtgacga ccagacgacg gaacgttttt gacagcccca ggcgtttaag
     9241 ccgcagaacc gggtatgacc acgcatcggt acgtaacaga tatccgctac ctgtaaaatg
     9301 aatccattct gattcaccga agtcactggt ctggtgtgac agcgagtaca accaggcgtt
     9361 gtccgtttcc gtgatatgtg cggtactgca gcgtatgccg gtaagggtag caaatggtgg
     9421 cgtgcattct gctgaggtct ggtcagattc atcaacaaca cgaagggagt agccgttatc
     9481 gcagaccatg ttaatcagtt cagcgagctc aataccatcg acgtcaacga caatgcgccc
     9541 tatgttcagt gcctgtacgt taacgctgtc ggcttccagt gtcagggcca gtttcatcat
     9601 ttcgcctccg gatacttacc cagggtaatg ttatttaccg ttctgtaatt gtcgcgggtc
     9661 atcaggccgg tcgcccggcg agcccggagg atatcaatac tgtttattaa ctgagagcgg
     9721 ggacaggtgc tgaatcccgg ctggtcggta cgcaccagcg cgtatttttc cacgagaaaa
     9781 ttaactgcat cacacagtga aatgcctgcc tcaatatgct gctcaatcac acgttcatcg
     9841 acaaacggtg tgtcattcag tgtgaggcca taatgctggt ccagcagtcg gatgagcagt
     9901 gtctgccaga tggtgacggg agacgggcag cgagatacct cccgtacatg tgtgtcggat
     9961 aatgttttca tcttaagttc tctgtaactg gtagtgctaa aggtgagagc ttcggggtta
    10021 tgaggtggtt gcgggtgctg gtgtcggata aacagccaga taaacgtaac cacaggagcc
    10081 gagggtgtcg gcttcgcagg tcagcccttt tgcatacagc gtgacggtat gctgatatcg
    10141 gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gcggaaaggc
    10201 ctggtccaga tggtatgcat ccgcattgct gaaccggcct ctgataccgg cgcggtctgc
    10261 aaggtaatac aggcggttac cctcttgcac cagacgagcg ccaaaacagg gcctcactgt
    10321 gcagggaagc ccccaccagg ggcggtcgtg attgtcatcg ggatgagtta tcccggagag
    10381 tttgtctgac acgataaaat cctctctgaa aagtggttaa gaatggttta acgatgacag
    10441 gtcagccggc gcgctgaccg gtttcggtga agatgtatgc attgatgaga atggcctcat
    10501 ccacagcggc gtctgaggtc atctggtcgt actcgtttcc cgggtgacgg aacatcagtt
    10561 cagggtgaca ctcatcagcc ggacatacgg gccaaaactg tccttacggc gttctgcaaa
    10621 cacggcaaga acgccgggaa tatcctgcac ttcacgacca gtatacgctt cagcactgcc
    10681 gtgccagcga tacttaccgg tacagaacgg aaaaagacgg gatgcaggat gctgttggtg
    10741 aatacgcatg gcttcaccac gggtgatgat tctcatgatg tgatacctct gaagacagaa
    10801 gataaaagtg aaaacaggtg tgatgtggtt gtgacgggtt aaagcagacc atgttcggca
    10861 aaggagaaaa cctggttgcc accgactatc agatggtccg gcacccggat atccaccagg
    10921 cccagtgcct gtaccagacg ttcggtgata agccggtctg ccttactggg tgtgacttca
    10981 ccggacgggt gattgtgcgc cagcaccacg gcagcggcat tgtggtacag ggcgcgttta
    11041 atcacttccc ggggatgaac ttccgtacgg ttgatggtgc cggtgaagag ggtttcaccg
    11101 gcaatcagct gattctggtt gttcagatac agcacccgga actcttcccg ctccagtccc
    11161 gccatgttca gaatcagcca ttcccgtgcc gcacgggtgg aggtgaaggc cacgccgggt
    11221 tcatgaagat ggcggtccag ggtttgcagg gcccgcagaa tgagactgcg ctcgccgggc
    11281 gtcatgtttc cgggcagaaa ggaaagctgc tgcattgtcc ctgcctccat tcagtcgatg
    11341 atgcgcataa tggcgctgca ttccggatgc tgcagggcgt aatcccgcag ccggtaataa
    11401 tggaccgtca tggcataaca ctccgtacga caggcatgat gactgtacgt catcagacag
    11461 gcagcaatgc cggcggcttc cgggctcatt tcagcgcggt taccgttcat ggcattgaac
    11521 agtacccatg tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg
    11581 ttcagggtgt acagattcca gataccaccg cagtagtctt cacacagacg gtccatccag
    11641 ccgaagacac ggggttccag ggtgacccac tgtggaatga ggccaaaatg ctgcggccag
    11701 aagctgatgc gctgttcatc agggacggga gtggcaacca gttgttgcag gccagtctcc
    11761 ggtgcacagg ccgaagcggt ggtgttctga gacaatgttt tcatgtatga aatccttgta
    11821 atgtgaataa atgagaggga gtaaaggtcg ggagctggca acatcacgtt catcactgac
    11881 cgtgatgacc cggtaatatc agggatgaat gatattcggc tgaaattccg gacagccgta
    11941 cgcgtaaccg ttccggtcac agacggatgg ccagatgcca ttgcctgtat actcagtctg
    12001 cacagactga tttctccggg acggtaaagc agacgggtac accaggcgca caggtcatca
    12061 tcagaaatgg aacgacaccc cggcagcgcc tccggggcgt cgggtgattc tggtgcctga
    12121 atcacgcctt cagggacatg ctgcctcagc gcaggctctc cagcagcgtt tctgccatta
    12181 cccacaatgc gcggttgagc ttaatatcgg tgtcgatgtt gtgaatggca cgggtgtgga
    12241 tacgttttcc ttttgcactg cggccggaaa tcccgccttt cagcatattc tcctggatgg
    12301 tctgatatgc gctccacagg tctttaccgt aatcctccct gcgtcgtggt gtcagaatgt
    12361 cagcggtggt gactggctga tgttcgtcac cataacggta agtcagtgcc gcctgtgcca
    12421 gcgcctggcg tgccggtggc ggcaggacca gcgactgcat ggcatcacgc ttctcctcta
    12481 tccggtcaaa cacgcccacc acctcgtaag ccccttcaat aactttctcc accacatttc
    12541 cccggtgtgg aacacgcaat tctcccagag actgaccgca gaccagtcca ttggtacaaa
    12601 tggcacgaaa atatccgggt aacatctggt agctggatga accgtcatga gagttgagca
    12661 gaataatttc agggacatgc tgaccggtta tctgtccggc ccgccgcaga cgcagcatgt
    12721 gttttgtgta ttcccggcga cccgggtcac gcacacgtgt ctggcaggcg aagaatggct
    12781 gaaagccttc ccgctgcagg ctttccagta cggtgatggt gggagtgtac gcatagcgtt
    12841 tactgcggga agtatgccgg tcttcaccga aaatactggg aacatagtgc atcagttctt
    12901 cgtgtgtcag cggacggtca cggcgtatct ggtttgcagc ataaccaaaa cgactggcta
    12961 gtcgcataat ttgctcctta tcggtggtta agatttactg gtgtaataaa tgaaaaagcc
    13021 acgtctcccg gagaagacgc ggcctgatgg acgaaattaa tgaggtttat tgtctgagaa
    13081 gaccgtcact ggcgaacctt ccggcgtcag tctctgcgtt gcggataacc cgacggaaat
    13141 gagttccctg gtcatcgtcg ataaacacat tgcggtaagc tgttgtgatg acaacagcct
    13201 gctcgcgtga gaaagggact tcgggcaata aacgtgacat acaaccagac ctgtatttgg
    13261 ttccctgaaa atataagtcc ctgtcatcat ggcagagccg tatttacttt tgatatcaat
    13321 aaattaataa cgcgaagatg aaccgcataa cttacgcctt taatttgttt tgtttacgta
    13381 gtccctgacc aggtacgcga attcgtgaac gccagtttca ccaaaccaaa tcaggtaacc
    13441 agggtcgatg ttgtaatagt aaccaacgac tttatgcgtt tttgggttat agagtgttac
    13501 gatatagtcc cagccgcagg cgtgggcttt acaaccgcca ccttctaccc aggtcgttcc
    13561 gttaatgttt acaggatacg caccaccatc aggaccatcc atatagccgt cttccccaaa
    13621 cattccgttt aacccaacag ggtttaatgc tttgtcgata gcagctctgt aggtagcgtc
    13681 accttcgtat aactcgtacg ttgttggaca tgcaccaggc tggtcagccc gggcttttga
    13741 gtcgcattta agtgtctgat ttacagtggc ggcgtttgtg tcaggactt
//
LOCUS       DAJTRQ010000105        13776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000105 DAJTRQ010000000
VERSION     DAJTRQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(194..1447)
                     /locus_tag="OO332_003104"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="OO332_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="OO332_003105"
     CDS             1554..2447
                     /locus_tag="OO332_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5943497.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="OO332_003106"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="OO332_003106"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5943498.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="OO332_003107"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="OO332_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5943499.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="OO332_003108"
     CDS             complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="OO332_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5943500.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="OO332_003109"
     CDS             complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="OO332_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5943501.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6683..7537)
                     /locus_tag="OO332_003110"
     CDS             complement(6683..7537)
                     /locus_tag="OO332_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5943502.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7539..8156)
                     /gene="dmsB"
                     /locus_tag="OO332_003111"
     CDS             complement(7539..8156)
                     /gene="dmsB"
                     /locus_tag="OO332_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5943503.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8167..10437)
                     /locus_tag="OO332_003112"
                     /pseudo
     CDS             complement(8167..10437)
                     /locus_tag="OO332_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(10446..11112)
                     /locus_tag="OO332_003113"
                     /pseudo
     CDS             complement(10446..11112)
                     /locus_tag="OO332_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            11095..12695
                     /locus_tag="OO332_003114"
                     /pseudo
     CDS             11095..12695
                     /locus_tag="OO332_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13189..13557
                     /locus_tag="OO332_003115"
     CDS             13189..13557
                     /locus_tag="OO332_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943504.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         3453 a         3512 c         3520 g         3291 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 cccgcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcaaaaa ggccacgaaa ctggggggga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gtgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaatga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaattgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
     5221 tcgctatgat taatcagatt gctaaccagc aatgccacga cggcggaaat aatcaccggg
     5281 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
     5341 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
     5401 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
     5461 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
     5521 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
     5581 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
     5641 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc agggggaaag gtttgtcgct
     5701 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
     5761 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
     5821 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
     5881 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
     5941 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc
     6001 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
     6061 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
     6121 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
     6181 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
     6241 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
     6301 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
     6361 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
     6421 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
     6481 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
     6541 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgctccc aacacgcgcg
     6601 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
     6661 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
     6721 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
     6781 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
     6841 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
     6901 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
     6961 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
     7021 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
     7081 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
     7141 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
     7201 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
     7261 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
     7321 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
     7381 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
     7441 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
     7501 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
     7561 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
     7621 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtacc gtgcttctgg
     7681 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
     7741 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
     7801 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
     7861 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
     7921 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
     7981 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
     8041 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
     8101 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
     8161 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgctag
     8221 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
     8281 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
     8341 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
     8401 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
     8461 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tgtagtggaa
     8521 gcccgttaac tgcagtggat aggttttccg caggggagcg tcccagccat caaaacctga
     8581 ggtatacgca ggaatgggat gaataatttc atcttttttc aattcccagg tatctgcaat
     8641 cttcgccagt cgttcagaat aaatttcgat tttccccgaa ggtgttttca acgggtttgc
     8701 ctgtggatct tcacggaatg cgcggaaagc gacgtagtgt tcttccgggc attttttctt
     8761 aaagatcccg gtcgttttca tctcctcgta gtcgggcatc tcagggttac gttccttcgt
     8821 tttcgcatgg agatatttga tccattcatg ctgactgcga ccttcagtaa aggtttgata
     8881 aacgtctggt cctaagcgtt tggcgacttc actcagcatc cagtagatgg gtttgcgttc
     8941 aaattttgct gaggttgcgg gttgggcgag gatcacatag cccatattcc ctgcagattc
     9001 atgagagata aggtcttctt gctctgttgg catcaggtca ggcaacagga tatcgcaata
     9061 cttagccgag gccgtcatga agtggtcaat gccaacaatc atctcgcact tgctgtcatc
     9121 ctgaagcacc tcatgggtgt gattgatgtc gccatgttga ttgatcaatg tgttactggc
     9181 gtagcaccat aaaaacttga tggggacatc cagtttttct tttccacgaa caccatcacg
     9241 ggtcgcggtc attttcgtac catggtcgat ggcatctgtc catgtaaaaa cggaaatctg
     9301 cgttttaaca ggattctcga gcatcgggaa ccattctacc cccagatccc agctaccttt
     9361 gcgtacacct gagttgccgc cgtttatgcc gacgttaccg gtaagaacgg aaagcatggc
     9421 aatagcgcgg gacgtttgtt cgccgttgga gtgtcgttgt ggcccccaac cctgacaaat
     9481 ataagcaggt tttgctgaac cgatctctcg tgccaactgg ataatttttt ctgccgggat
     9541 gctggtgatt tttgctgccc attccggcgt tttagctatg ccgtcaggcc cttcgcccag
     9601 aatataggct ttataatgcg cgttacgtgg tgcgttggcg ggcagcgttt tttcatcgta
     9661 accaacacaa tatttgtcga gaaatggctg atcgaccatg ttttcagtaa tcagtaccca
     9721 ggcaatcgca caggccagtg cgccatcggt gccagggcga atgggcagcc attcatcttc
     9781 acgccctgca gctgtgtcgt tatagcgtgg atcgatgacg atcatgcgtg cgtttgaacg
     9841 ttcgagggct tgctcgacgt agtaagtgac accaccgccg ctcatccgcg tttctgccgg
     9901 gttatttccg aacataacga ccagtttcgt attggcgata tcatccgggc tgttgccatc
     9961 attggcaccg aacatataac tcattgcggc actgatctgt gcggtactgt agctgccata
    10021 gcgactgaga aaacccccgc aagagttcat cagacggtac gggacgttcg agttggtgat
    10081 atttccgcca tctacgcctg ttccgtacag gacatgtaca gcctcattgc cgtaatcttt
    10141 caggatccgc cgaagattat cactgatggt atccagggct tcgtcccaac ttatccgttc
    10201 aaatttacct tcaccgcgct cgccgacgcg cttcatggga tatttcaacc tgtcaggatg
    10261 attcatccgt cggcggatag agcgcccgcg taaacatgcc cgaacctgat gattaccgta
    10321 aacgtcgtca cctgtcgtat cagactccac ccagtacacg gtgtcatctt tcacatgcaa
    10381 acgtagcaga cagcggctcc cgcagttaac ggtgcaggaa ctccagaccg ttttctcttc
    10441 tacccatctt tttccagttc ccaggtacgg gcaatttccg ccagcttgct ggaataaatt
    10501 tcaatcttac cggaaggcgt tttaagtgga tttgcctcgg ggtcgtcacg aaatgctttg
    10561 taggcgacaa aatggccatt gggatcttta cgcttataga tacccatttt tttcagttcg
    10621 tcgtaagacg gtaacgccgg atctttggca agcattttgg cgtacagatg ttgtaaccat
    10681 tgttcctgcg tgcgaccttc tgtgaacttt tgatagacgt caggtccaag acgtttcgcg
    10741 acttcactca ggatccagta aatcggttta cgttcgaatt tttcgctggt gactggctgg
    10801 aggaaaatga gatatcccat gttaccggcg tagtcgttag gaataatatc ttcctgctca
    10861 acggtcatca ggtctgccag cagaatgtcg gcatattttg ccgtatgagg tcataaagtt
    10921 ttcgatgacc acaatcattt cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt
    10981 gatgtcagaa tgctgattaa cgagggtatt tcccgcgtag ttccagatgg actccactgt
    11041 cagacggatg gatggacttg aagtagttga ctcaacgcta cttaaggtcc tgatatgact
    11101 gacattaacc atgataatac tgtatatgca tccagttctt tctctgggtc tccctatctg
    11161 tatcagcaag tctacacctc aagtcctgac acaaggctga cacaaatcaa ggaccttgca
    11221 gtcccgcact tgctcgcaag gctcgctacg ccacagttag tccgtagagc taacggtcga
    11281 tatactatcc gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc
    11341 agcacagaca ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc
    11401 ttcatgttgg ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg
    11461 gctgagcagt tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact
    11521 ttggtggacg agaagtccaa cctgagggag ctagccgcta cgcaagcctt gagtctggac
    11581 cagcagaagg gcataaggtt agcgcttgaa gtcctgaccg cagcacagca gagggtagac
    11641 actggtgata cctctggtct gattaagctg attgatgata ataaccatac tgattactca
    11701 actataggcg actctacgtc aattctgaag aatgagcagg gggatagcca gcagttttca
    11761 cacaggagcg ccaaccatct gtagtttttt caagcctagt gtcctcgctg ctggctgaga
    11821 aggcccagac cctgaagtca tccagctaca aggacctgtc gtcctcactg aataccgtct
    11881 ctcggttctt acctgaagac atggacctga tgtcccgctc tgagtggctg gctgtcaggg
    11941 acgctatgct ggcttctgag gtgagaccgt cgacgattaa caagctgctg actaaggcga
    12001 agatgtgtct tgattacggt ctgatgaatg ggcaacttga gggccgcaac cctattgaac
    12061 ggatgaagct gaccaaggat atcgactcca agcgcagagc gttcacagac gaggagctgg
    12121 agagacttct ggtccgtgtt gaggctgagt atcagttcac acgacacacg gctcacacga
    12181 cctctgaggc ccgtagatgg gcgtctctgg tttctgtagt tactggcgct cggtctgctg
    12241 aggtctgcca cctgactaaa cgtgacattg taaccttaga caatggactg gtctgtatcg
    12301 atataaacga agatggggac ggtaagtctg tgaagaacaa gcattcagtc cgacttgtgc
    12361 cgctgaccga tggcgttcat gggttcgacc ttgagtcttt cctcaaatgg gtagacatgc
    12421 agcctgatga tggtcctctc ttcgggatga ctccgagcgc ctactctagc tggtttaact
    12481 caagggtcct gactgaggcc ctaggagact ctcaggatgt ctctctccac agtctgagac
    12541 attggctggc gacccgaatg aaagagcgtg gagtcaatct ggtggacgct caaggcatct
    12601 taggtcacag cagtcagtca attacgtatg acctgtacgg gaaaggacac gctgtgggta
    12661 ggctggctga tgtgcttaag acagctttac tttaagtagg gagacccaag gtggagacca
    12721 tatacggctt gatggttgag accttgggtc actgaagtct gactgaagtt agggagggtg
    12781 ataataatca taatccctcc tgactatagg cccactggtc cgccttcggc tgtccgactc
    12841 taagtctgtc tgtggtctta tttaaactgc gtcctaaact ccagtccctc caaattccta
    12901 tggtctcctc ttgacattcc tgtttaactg tgagtcgcct ctggtctctc tttatatata
    12961 cttccttaat tcctgaggcg tctttagtcc ttgacatttg gttcaacctg tgagtcacgt
    13021 ccggtccccc tctttatatt tacttcctta attcctaggg tcgccttaat ccgtgacatc
    13081 cagtccagcc tgtgagtcac atcctgtcgc gctactgtat gcatcaccag tgatggactt
    13141 gattatcact ataggggaac caactaattc aactacagga gatactctat ggcacgacca
    13201 atcgcaactc acgacaacac gttcaccaaa gcctacttgc agcagcactg cggggacctg
    13261 ctgtcattcg atggtcaggg cgacctgagt ggttggctgg atgatgtcct tactggtgct
    13321 ggtcgcctga acgaatcaat ggcaagcaat accaagcctg tcagcccata cttgattctg
    13381 acccaactac taacgcacga cacattaact gtaagcgctg tgcaggagtc cctgagccgt
    13441 aagcgtgttg ccttgggtga acctatggtt tcaactcgct acgctcgcta cgtctatgct
    13501 accgtcgtca gcgccagtaa atccgtccag taccacgcca gtaaagctgg ttcatgatta
    13561 cggaccctaa actatcacta tagggagacc agcctccgcc tctgagtctg atgcgtcaga
    13621 acatgcgagc atctggtcaa ccgacattaa gtatctctcc taagtaatca aggcatgtga
    13681 ccgcctctgt cagacctcgt gtatcgacct ccagctcgtt agcgttcttc agtcatctca
    13741 gttagtagct cgtctactca ctacattggc ttaagt
//
LOCUS       DAJTRQ010000106        13630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000106 DAJTRQ010000000
VERSION     DAJTRQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(62..978)
                     /locus_tag="OO332_003116"
                     /pseudo
     CDS             complement(62..978)
                     /locus_tag="OO332_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1219..2019
                     /locus_tag="OO332_003117"
     CDS             1219..2019
                     /locus_tag="OO332_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5943505.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2097..2867
                     /locus_tag="OO332_003118"
     CDS             2097..2867
                     /locus_tag="OO332_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5943506.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2915..4273)
                     /gene="mltD"
                     /locus_tag="OO332_003119"
     CDS             complement(2915..4273)
                     /gene="mltD"
                     /locus_tag="OO332_003119"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5943507.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4345..5100)
                     /gene="gloB"
                     /locus_tag="OO332_003120"
     CDS             complement(4345..5100)
                     /gene="gloB"
                     /locus_tag="OO332_003120"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5943508.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5134..5856
                     /locus_tag="OO332_003121"
     CDS             5134..5856
                     /locus_tag="OO332_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5943509.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5853..6320)
                     /gene="rnhA"
                     /locus_tag="OO332_003122"
     CDS             complement(5853..6320)
                     /gene="rnhA"
                     /locus_tag="OO332_003122"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5943510.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6385..7116
                     /gene="dnaQ"
                     /locus_tag="OO332_003123"
     CDS             6385..7116
                     /gene="dnaQ"
                     /locus_tag="OO332_003123"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5943511.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7249..7325
                     /locus_tag="OO332_003124"
     tRNA            7249..7325
                     /locus_tag="OO332_003124"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7283..7285,aa:Asp,seq:gtc)
     gene            complement(7456..8502)
                     /locus_tag="OO332_003125"
     CDS             complement(7456..8502)
                     /locus_tag="OO332_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5943512.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8513..9508)
                     /gene="tssG"
                     /locus_tag="OO332_003126"
                     /pseudo
     CDS             complement(8513..9508)
                     /gene="tssG"
                     /locus_tag="OO332_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9505..11388)
                     /gene="tssF"
                     /locus_tag="OO332_003127"
     CDS             complement(9505..11388)
                     /gene="tssF"
                     /locus_tag="OO332_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5943513.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11404..11898)
                     /gene="tssE"
                     /locus_tag="OO332_003128"
     CDS             complement(11404..11898)
                     /gene="tssE"
                     /locus_tag="OO332_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5943514.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11895..12725)
                     /locus_tag="OO332_003129"
     CDS             complement(11895..12725)
                     /locus_tag="OO332_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCR5943515.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12712..13626)
                     /gene="tagK"
                     /locus_tag="OO332_003130"
                     /pseudo
     CDS             complement(12712..13626)
                     /gene="tagK"
                     /locus_tag="OO332_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3123 a         3546 c         3635 g         3326 t
ORIGIN      
        1 atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc
       61 cttaactgat cggcattact cctccgggag ctgtttttac atgctcgcta aggaaatcga
      121 taaaagcccg gatgcgcgta cttaccgcac ggtcgctgta atagacggca ctgaatggca
      181 tttccactgg caacacttta tctgccatta actccaccaa ttatccgcga gcgatttctt
      241 tgtcgatcat gtagtcggac aaacacgcaa tcccgttgcc actcaggcaa agctgtttca
      301 gtgtttcccc actattggat gacaaaccgt acttcacctc atgtaattgt ccatcactac
      361 aggctatcgg ccaggtattg agggaagcgg gttcagtgaa tcccaggcaa acatgttgct
      421 ttaaatcgtc gatcgtttct ggcttcccgt agcgggaaat ataatcgggg gaggcgataa
      481 tttttcgata actgttaaat aacggcctgg cacgtaagct ggagtccgtt aacgtaccag
      541 cgcgtatcgc gacatccact tttctttcga tcaaattaat aatcgtttcg gaggagacta
      601 gcgataaagt gacttccgga tagcgttcac ggaaaggctt aattaacggc atcagaaagt
      661 gcagcaccac tggagttgcg gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct
      721 ccataatttc tgattctgcc gctgccatct cctgcaaaat tgactgtacg cgacgaaaat
      781 aacgctcgcc ttcttccgtc aggctaagtt gccgcgtggt ccgattaagc aggctaacgc
      841 caagtttcat ttccagcttt ttcaccgccc ggcttaccgc tgagtttgct tgccctaaaa
      901 ttgttccgct gcccggctaa agctgccgct ttcgacgacc gaaacaaaaa tggcgagttc
      961 ttccgacgtg gctttcattt ttgctcctgt tgcaaaatag atgagatatt ttgaatttat
     1021 ttgtcattaa accatcagga tgcgtgatat gtcatccgat ttaatgttct caataatgag
     1081 caaatttctg accggtgtaa gcacttgctt acataacaat atacaattgc tcgttgaaag
     1141 agtgagctaa aatccctata acagtagaac cctcccgagt gcggaagggt tgacgtaata
     1201 gaggtttcaa agtcaaaagt gcgaaaaaac acctatgcca tgcgctatgt tgccggacaa
     1261 cctgcggaaa ggatcttacc gccggggtct tttgcgagca tcggccaggc attaccacct
     1321 ggagaaccgt taagtaccga agagcgtatt cggatcctgg tgtggaacat atacaaacag
     1381 caacgcgctg aatggttgtc ggtattaaag aactacggca aagatgcaca tctggtgtta
     1441 ttgcaggaag cgcagacaac gccagagtta gtacagtttg cgaccgctaa ctatcttgcc
     1501 gccgatcagg tgcccgcttt cgtgctgcca caacatcctt ccggtgtaat gaccctttcg
     1561 gcggcgcatc cagtgtattg ctgcccgtta cgtgaacgag aacccatttt gcgtctggcg
     1621 aagtcggcac tggtgacggt ctatccatta cctgacaccc gcctgttgat ggtggttaat
     1681 atacacgccg tcaacttcag tctgggcgtg gatgtctata gtaagcagtt acttcctatt
     1741 ggtgatcaga tagctcacca cagcggcccg gtcattatgg cgggagattt caatgcctgg
     1801 agccgtagaa ggatgaacgc gttatatcgc tttgcgcggg aaatgtcgct gcgccaggtg
     1861 cgttttaccg atgatcagcg ccgtcgggcg tttggccgcc cgctcgattt tgttttctac
     1921 cgtggtctga acgtcagcga agcttctgta ttggttacgc gcgcttccga tcacaatccg
     1981 ctactcgttg aattcagtcc cggcaagcct gataaataag gtatgtcagg tctgccacag
     2041 ggcagaccaa cgtttggcgc tgtcctttta tactcaaaca acaaaaggac agcgcaatga
     2101 caacacaatc ccaccatgac cacgtagaaa agcagtttag ctctcaagcc tgcgaatatc
     2161 tgaccagtac cgtacatgca tccggacgag atttacagcg cctggcggtg cgtctggctg
     2221 attatcctgg tgcaagtgta cttgatatgg gctgcggagc agggcatgcc agctttgttg
     2281 ctgcgcaaaa cgtgagcgcg gtggtggcgt atgacttatc tgcccaaatg ctggatgtcg
     2341 tggcacaagc tgccgaagcc cggcaactga aaaatatcac cacccgccag ggatatgctg
     2401 aaagtctgcc atttgccgat aacgcatttg atattgttat cagccgttat tctgcccatc
     2461 actggcatga tgttggtgca gcactgcgtg aagtgaaccg gatattgaaa cctggcggta
     2521 ggatgattgt gatggacgta atgtctccgg gtcatccagt gcgcgacatc tggttacaga
     2581 cggtagaagc attacgcgat acctctcacg tacgaaacta cgccagcggt gagtggttga
     2641 ggttaatcaa tgaagccaat ctgatagttg ataatttaat tacagataag ttaccgctgg
     2701 aattttgttc atgggtcgcg agaatgcgta cgccagaagc gttagtagac gctattcgca
     2761 tttaccaaca gagcgcatcg acagaggtga gaacgtattt tgccttgcag aatgacggct
     2821 ctttcaccag tgatatcatc atggtagaag cacataaagc ggcataaata aaaaaggcac
     2881 cgggggaatc ggtacctttt tattatctgg tttgtcagga atctggcatg ttgttgtttt
     2941 tcacaaacaa cgtcagcttg tcgcctggtt gcagattcgc agtgtcgctg ttccagcgca
     3001 tcacatcttt gatgttcacg ccgtggcgtt tagcaatgct tgaaagcgaa tcgcctttgc
     3061 gcacacgata cgtaatgcta tcgctgttgt ttgccagtcg ctgtgcgcta ctgcctgcac
     3121 caatcgtcaa actttggcct ggcttcagct tagatccgcg cagtttgttc cactgctgca
     3181 aatctttggt gcttacgccg agacgtgaag cgatgcttga aagcgtgtcg ccagagcgta
     3241 cggtgtaaac acggctgtta agcggcgtat tgtcggcaac cagcgtcgac tgtacagcag
     3301 caatttcgcc tgaagcgaga gattcacgca gttgatctgc atgcttcttt ggcaccatca
     3361 cgtactgcgg cccacttgcg cccagcgtgg agcctttcac gccagcgttg aatgtcttca
     3421 gcttgctgac ggaaatcccc gccatatctg caaccttcgc catttcaacc gggctactca
     3481 ggtgcacacg cgccagagca cggctttcat cggtcgttgg cagacgtacg ccataacgct
     3541 tgctgttttt gagaatatca ctcaatgcca gcattttagg cacgtactgc ttcgtttcct
     3601 gcggcaacgg taacgaccag aagtccgtgg atttcccacg cgctttgttc gttttaattg
     3661 ccttcatgac ccgaccttcg ccgctgttat aagccgctac ggtcagaagc cagtcgccgt
     3721 caaacatttt gttcagacgc tgcatcatat tcagcgcggc agttgttgaa gcaacaacat
     3781 cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata attgcgcccc gtgctcggaa
     3841 tgatctgcca gatgcctgcg gcattggcgc cagacgttgc gtgaggatca aaagcgctct
     3901 ccactatggg tagtagtacc agttccatag gcatgttacg ttttttaact tgccctgcta
     3961 tccagtacat atacggctct gcccgtaaag ttacatcgtg gagatagctc ttattgcgta
     4021 aatatttctg tttctgttcg cgaatccggt cattttccgg aattcccatc tttagctcgt
     4081 cgccaatgaa agcccacaag tcaccatctg gcgcgataga cgtcccatcg tccatccatc
     4141 gtgcctgact tgtaaacttt gctgcttccc cttgaccagc tgcagaaagg ctctgtgcgt
     4201 gctgttgaac gttgccggta ctctggcaac ccacgagcag gacagaggcg agtaatatcg
     4261 cttttgcctt catgtgtgtg tcaatagttg cttaaaagac gaccgatcat aacggcgaac
     4321 ggagccgatg acaagaaagt tttatcagaa cctatctttc tttgacctta accatgcaaa
     4381 acgctcttca ggttgttgca ataatgtttc ttcattaatt acattaatta aatcaatatc
     4441 ttccgttctt aaaaaaacat taatttgccg ctcatttttc agaattacgg gtagtgttat
     4501 ttgatttttt gcccgtaact ccttaacttt acgataataa tcatttatgg acaaatcgtg
     4561 cggaaaaata ctcaaagcaa acttcatatt tgataacgta tattcatgag cacaacatac
     4621 caatgtatcg tcaggtaacg cacttagctt tttaagtgat tgatacattt gtaaagctgt
     4681 cccttcaaac aaccgaccac acccaccaga aaacagcgtg tcgccgcaaa atagataagg
     4741 tttactgaag taacagatat gtcctaaagt gtgacccggt gtggtaatta cactaaattc
     4801 atgccctaaa acgaaggcag tttcgccatc tttgactacc tgtgttgttc ccttatcttg
     4861 tgtctcttgt ggaccataca ccacaatttg cggaaacttt tccaccagtt cttttacgcc
     4921 gccaacgtga tcgtggtgat ggtgggttag aaatatggcc tccggttgcc agttattggc
     4981 ggtaatggcg tttaatactg gctctgcgtc tccgggatcg acaatcaggc agcgacctgc
     5041 ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg ggaatactgt taagattcat
     5101 agattacctc tcagtgtaaa acggaaggtt gtgatgaaac cggcaagagt ccctcaaact
     5161 gtcgtggctc ctgattgctg gggcgatttg ccctggggag agctttatcg caaggcgctg
     5221 gagcgccagc tcaacccgtg gttcactaaa atgtatggtt ttcatctgct taagattggc
     5281 aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt ctcatcaagt gaatgtttct
     5341 gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc atcttccttt tgccgataaa
     5401 tccgttgata tttgtctact ggcacatacc ttgccgtggt gcaccgatcc gcatcgttta
     5461 ttacgtgaag ccgatcgggt actgattgat gatggctggc tggtgattag tggcttcaac
     5521 cccatcagtt taatgggatt acgcaaactt gtgccggtat tgcgcaaaac ctcgccctat
     5581 aatagccgga tgtttactct gatgcggcaa ctggactggc tctctttgtt gaattttgaa
     5641 gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca aacacggagg aaaactattg
     5701 aatgcgcata ttcctgcgct tggttgctta caacttattg ttgcccggaa acggactatt
     5761 cctttaacgc taaatccgat gaaacagagt aaaaacaagc cacgaattcg ccaggcggtt
     5821 ggagccaccc ggcaatgtcg taaaccacag gcttaaactt cgacttggta gcccgtatct
     5881 tccagtgtgg gattcatcgc cgcggcacga gccagttcat cacagcgttc gttttccggg
     5941 tgtccggcat ggcctttaac ccattcccat ttgatttgat gctgccccaa tgcagcatca
     6001 agacgttgcc agagatcgac attttttact ggttttttgt ctgctgtttt ccagccacgt
     6061 tttttccagt tatggatcca ctgggtgata ccctggcgga catactggct gtcggtactc
     6121 aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga caatagcggc catcaactcc
     6181 atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg ttttctcgcg tccgcgatag
     6241 cgtaaaatag cgccgtaacc cccaggtcca ggattaccca gacacgaacc atcggtgaaa
     6301 atttctacct gtttaagcat ctctggtaga cttcctgtaa ttgaatcgaa ctgtaaaacg
     6361 acaagtctga cataaatgac cgctatgagc actgcaatta cacgccagat cgttctcgat
     6421 accgaaacca ccggtatgaa ccagattggt gcacactatg aaggccacaa gatcattgag
     6481 attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca ataacttcca tgtttatctc
     6541 aaacccgatc ggctggtgga tccggaagcc tttggcgtac atggtattgc tgatgaattt
     6601 ttgctcgata agcccacgtt tgccgaagta gccgatgagt ttatggacta tatccgcggc
     6661 gcggagttgg tgatccataa cgcagcgttc gatataggct ttatggacta cgagttttcg
     6721 ttgcttaagc gcgatattcc gaagaccaat accttctgta aggtcaccga tagccttgcg
     6781 gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg atgcgctatg tgcccgctac
     6841 gaaatagata acagtaaacg aacgctgcac ggggcattac tcgatgccca gatccttgcg
     6901 gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg cttttgcgat ggaaggagag
     6961 acacagcagc aacaaggtga agcaacaatt cagcgcattg tacgtcaggc aagtaagtta
     7021 cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg aagcccgtct cgatctggtg
     7081 cagaagaaag gcggaagctg cctctggcgg gcataaatac atgtaaaagg cgctaaaaat
     7141 agcgacttgg gcgatttttg cagcaaacga ttcaaaagat gagaaaaatc gttgacgaag
     7201 gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa cacaacgcgg agcggtagtt
     7261 cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg ggttcgagtc ccgtccgttc
     7321 cgccaatatt cagaaagccc gaatcagtaa tgattcgggc tttcgtcgtt ttggtgaagt
     7381 gagaattttt cactccgaag taaaacggat atgatttaaa aaatggccag acattataaa
     7441 ttctggctac ttctgttact ccagcgcatc ccatttctcc agcagttcta ctggaatttc
     7501 atttattacc tccgagacgc gttttccgac caggcgttct gcctggcgca gcaaaggaat
     7561 ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg atgacacgca ggcgttccag
     7621 cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg gtcatcggtg atatttcctc
     7681 cgcgatatgc accggtagcg caatctgctc tgcctgcatc tgttcagtat gaacgatggt
     7741 ggcgttttcc ggctgctcag cctgtggttg tggcaggatc tccggcgtgg tgctctgcac
     7801 tgctcccggt aacagggcca gcagttgcgt cagacgggag aagtccggtg cctggtcatt
     7861 cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc tctgctgcct ggcgaaaggc
     7921 atccagcggc agtgtgccgc gcgcttccag gtctgccagt tgctggcgta ccgattctgg
     7981 tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc tccacatccc gcacctgaag
     8041 gcgcatggca gcattatttg acagtgtaat gccacgaata tccgccatta caccttcgtg
     8101 gtccagcaac gccgccagcg cgttactgcg tgccaccgca gcatcttcgg cggtgatatc
     8161 ttcagtcgcg agcagttgag gatggatggc gtccgggtac gtggagcaca acgtttccag
     8221 cagcgtgagc gcctccgaca gtcccctggc accggcctgc tggatacggc agcgcagcag
     8281 gacaaccaga atgcggatat ctttgctgcg catcagcaga cgatgggcgt cccgctcaag
     8341 ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg ccatattgcg cttcttcacg
     8401 cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg tactccagat ccgggccgca
     8461 ggggcattcc gccgaaattg ggtcagacag gaatatttcc atttaattat cctcagtgcg
     8521 ccaggtagtg ttctggttcg aaaatcatgc ccgtcaccgg gttgtcgtgc atggctttac
     8581 ccgcccatgc agtccaaccc agttggtacg gaccaccggt aacagccgga ggggcggcgt
     8641 ggggctttag ctgtagctca atttcccagc agtattcata accactaaag gcgcggacca
     8701 gttctgtcag gacgggcagg ttattgctgc caggcagaaa acgtcggtaa tcctccagcg
     8761 tcagcgggcc gataaccagc cggaacttat actgcatgtc cggcacggcc tggccgatga
     8821 gtgcactatt gccaataacg gaagaggggg caggcatccc cagccgggtg atctcatcgt
     8881 ccgccacggc tatccagtgc aggacatact cctgtatccg gaaaggtaca ccgaaatagt
     8941 gcgccagcgt ggtggccaga ccgtccggat tgcgggactc acgcaccaga tgggccgatg
     9001 ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct ctcccgcaaa tcctgcccgc
     9061 tgaggctggc gacataaaag gcaaagcggt catgttgcgg tttatccaga ccgcccccag
     9121 cggactgggc gctgcgccat gcctggtaaa actgcgtcag ccagcggtga tgaaaaagat
     9181 cagaaaaacg ggtcagcgtc ggatcgcgtc ggctttcagt ccggttacgc gccagttcgg
     9241 tgtagtgcag cggcagtgga ccgttcggcc cccacagccc gaggctgtac agactgatgt
     9301 gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag gtcagcgcgg
     9361 gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcta ggtgttgccc gccggaacgg
     9421 tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaatgc caggggggtt
     9481 cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc tgacaggcca
     9541 ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg tattaatgga
     9601 aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac tgatgccgga
     9661 aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga ccagtagtcc
     9721 ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc tgcgcacctg
     9781 gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc gcaacgcctg
     9841 gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata actggcggat
     9901 caaccgccat gccatctcac gttcagccag cgggggctgc ggcgagcgtg gcgggcggat
     9961 aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc ggccattgcg
    10021 ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga tgtggcgcag
    10081 attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt ccgagcccgt
    10141 atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg gctcccggcg
    10201 cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt agagcgggcg
    10261 aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga ccgagaacac
    10321 ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg tgctgtgcgt
    10381 gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac agaacaggct
    10441 gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt tcagcaggat
    10501 aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc ctgtcggggt
    10561 gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat gaccgtgaaa
    10621 cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt acataacggg
    10681 ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag ccagggtcgc
    10741 gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct cgccgcagag
    10801 ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac caaaggtgcg
    10861 cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc ggtgcagggc
    10921 aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg gccacagcgt
    10981 tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa tggcggagaa
    11041 aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt ccgtatcggg
    11101 atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc tgaccacttc
    11161 cagcgggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg tgcgggcggt
    11221 caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat ccgttccctg
    11281 catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc ccgtttcccg
    11341 cagatagctc agttcgcggt tgtagtatcc cagtagccgt ggatccatcg gtctctctct
    11401 gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta cccggaatgc
    11461 agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg tgttatggcc
    11521 actctggcgg cgctcatcct gaagtgggat gatttgcagg gtggacgctt tcagccgggg
    11581 ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact tgtgctcata
    11641 cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg ccgcgtacgg
    11701 ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa atgacaaatc
    11761 ccggcggatg atctccttaa tctgaaccgg gctgacgctg ataccgtggt cgcgtttctg
    11821 gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat tgcctggacg
    11881 ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca ggtggacaaa
    11941 tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag cgcctgtacc
    12001 agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag ggagtcggca
    12061 gccttttcag agccgctgta gcggggaaac agccaggcac tgtgtgtatc gccattgttc
    12121 agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg cggcgatttc
    12181 agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat cagttcgagt
    12241 accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc attccactgc
    12301 ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc ttcctgcgct
    12361 tcaccacggg cgttacaggc gagggtggtc agcagatcct ccatccaggc gggcggcggg
    12421 agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat ctcacagcgg
    12481 atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg ggcgcatagc
    12541 tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg aaacaggctg
    12601 tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat ctgaatgccc
    12661 tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc gttattgttt
    12721 gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggttgt caatgccaat
    12781 gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg tttccggggc
    12841 gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag gccagatagt
    12901 ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta acccaccagt
    12961 gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat ccagatcctg
    13021 cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat cttgttcggg
    13081 agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc ctttttccgc
    13141 accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat tttgtgatgt
    13201 cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt ccgacgtgat
    13261 ggcagtctgc gatatcgttt ttactgtgct caggtccgga gatgatggat tgccatgtac
    13321 aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac gcatacggtg
    13381 atgaggttcc acaatctggt cgtttaccgt acagcaatat tcggtagact gattgataat
    13441 ccaccatgct tcttcgtggc gggtcagagt gagggcaatc tttacatcat cattcacctt
    13501 aaaaacagga atccagggag aggtaacggt gaagataatt tgtgttcctt cagtcgcgta
    13561 aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg agtccccacg taatatttgg
    13621 tttcattgtg
//
LOCUS       DAJTRQ010000107        13454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000107 DAJTRQ010000000
VERSION     DAJTRQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            178..1431
                     /gene="kch"
                     /locus_tag="OO332_003131"
     CDS             178..1431
                     /gene="kch"
                     /locus_tag="OO332_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5943516.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1486..1659)
                     /locus_tag="OO332_003132"
     CDS             complement(1486..1659)
                     /locus_tag="OO332_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5943517.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1802..3262
                     /gene="cls"
                     /locus_tag="OO332_003133"
     CDS             1802..3262
                     /gene="cls"
                     /locus_tag="OO332_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5943518.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3297..3626
                     /locus_tag="OO332_003134"
     CDS             3297..3626
                     /locus_tag="OO332_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5943519.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3679..4683)
                     /gene="oppF"
                     /locus_tag="OO332_003135"
     CDS             complement(3679..4683)
                     /gene="oppF"
                     /locus_tag="OO332_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5943520.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4680..5693)
                     /gene="oppD"
                     /locus_tag="OO332_003136"
     CDS             complement(4680..5693)
                     /gene="oppD"
                     /locus_tag="OO332_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5943521.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5705..6613)
                     /gene="oppC"
                     /locus_tag="OO332_003137"
     CDS             complement(5705..6613)
                     /gene="oppC"
                     /locus_tag="OO332_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5943522.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6628..7548)
                     /gene="oppB"
                     /locus_tag="OO332_003138"
     CDS             complement(6628..7548)
                     /gene="oppB"
                     /locus_tag="OO332_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5943523.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7634..9265)
                     /gene="oppA"
                     /locus_tag="OO332_003139"
     CDS             complement(7634..9265)
                     /gene="oppA"
                     /locus_tag="OO332_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5943524.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10003..10650)
                     /gene="ychE"
                     /locus_tag="OO332_003140"
     CDS             complement(10003..10650)
                     /gene="ychE"
                     /locus_tag="OO332_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5943525.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11127..>13454
                     /gene="adhE"
                     /locus_tag="OO332_003141"
                     /gene_synonym="adhC"
     CDS             11127..>13454
                     /gene="adhE"
                     /locus_tag="OO332_003141"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5943526.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYA"
BASE COUNT         3520 a         3334 c         3125 g         3475 t
ORIGIN      
        1 tgatgtgagc atgttcagga aaaaagccct ttcctgacgc agagttgctc actcaaaacc
       61 tttgattact gcggataaag tcatctacta ttgtttaaca cattcacgcg ctgcatgtat
      121 gttcagaaaa tgtgcacgct aagataatta aacaaaattg tggttacaga ggtatcagtg
      181 agtcactggg ctacattcaa acaaacagca acaaatttat gggtgaccct acggcacgac
      241 attctcgcgc tggccgtctt tttaaatgga ttgcttattt ttaaaacaat ctatggtatg
      301 tcggtcaatt tgcttgatat tttccatatc aaagcatttt cagagttgga tctctccttg
      361 ctggcaaacg ccccactatt tatgctcggc gtctttcttg tcctgaactc cattggctta
      421 ctgttccggg caaagctcgc atgggcaatc agtatcattt tgttgttgat agcgctaatt
      481 tacaccctgc atttttatcc ctggctgaaa tttagtattg gattttgcat ttttacgctg
      541 gtgtttttgc tgatactgcg caaagacttc tcccacagta gcgccgcagc cgggacaatt
      601 tttgcattta ttagtttcac gacattactg ttttactcaa cctacggtgc actttattta
      661 agcgaaggtt ttaatccgcg aatagaaagt ttgatgaccg cgttctattt ttcgatagaa
      721 accatgtcaa ccgtcggcta tggagatatt gtccctgttt ctgaatcagc acgattgttc
      781 actatttcgg tcattatttc cggcattacc gtttttgcca catctatgac ctcaattttt
      841 ggtccgctta tccgcggggg attcaacaaa cttttaaaag gaaacaatca tacaatgcat
      901 cgtaaagatc attttattgt ttgcggacat tcgattctcg ccatcaatac gatcctgcaa
      961 ctgaatcaac gcggacaaaa cgtaacggtt atcagcaact tgcctgaaga tgatatcaag
     1021 caacttgagc aacgcttagg cgataacgct gatgttatcc ccggtgacag taatgacagt
     1081 tcagtattaa agaaagcggg aatcgatcgt tgccgggcca ttctggcgct gagtgataac
     1141 gatgcagata acgcgtttgt tgtactctcg gcaaaagata tgagcagtga tgtcaaaaca
     1201 gttctcgccg tcagtgatag caaaaacctg aataagatta agatggtaca tccggatatc
     1261 attctctcac cgcaactgtt tggcagcgaa attctggcgc gagttttaaa tggtgaagag
     1321 attaataatg atatgctcgt ttcaatgttg ttgaactcag gtcatggtat tttcagcgat
     1381 aacgatgaac aagaaacgaa agctgacagt aaagaatcgg cgcaaaaata gagtttaacg
     1441 acggaagagt aaacttattg ggcaccgcaa tggtgcccag tacatttaga tattgtagat
     1501 cgcaaacaat aacgagttac gctgtttgtt tagaatgcac ttcctgatgc tgtggatacg
     1561 catatttcgg cgcgattgcc cctcaagcca acgcgattta cgtcggctag cctgacgctg
     1621 catgcgccag cgccccactt ccgttctact ccgcttcatg ttaactactc tatgcaataa
     1681 cagaatggtc attatacccc cgcccccgac gctgaccagt gcttttcccg cgtttttatt
     1741 tgccagatga atccatatgc gtaaactcat aacaatgcgc tttcaaaagg atttctaatc
     1801 tatgacaacc gtttatacgt tggtgagttg gttggccatt ctgggatact ggttgctcat
     1861 tgcaggcgta actttacgca ttctaatgaa acgacgcgca gttccctccg cgatggcctg
     1921 gctgttgatt atttacattc tgccgttagt cggaattatt gcctatcttg ccgttggcga
     1981 gctccattta ggcaaacgcc gcgctgagcg cgccagagcg atgtggcctt ccaccgcaaa
     2041 atggcttaac gaccttaaag cctgtaagca tatcttcgcc gaagaaaata gcagtgtcgc
     2101 tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc gctggggtca aagggaatca
     2161 gctacaactg atgaccgagt cagatgatgt gatgcaggcg ttaatccgcg acatccagct
     2221 cgcgcgccat aatattgaga tggtgtttta tatctggcag cccggcggca tggcggacca
     2281 ggtggctgaa tcattaatgg cggctgcacg gcgtggtatt cattgccgat tgatgctcga
     2341 ctccgccggg agtgtggctt ttttccgcag cccgtggccc gagctaatgc gtaatgccgg
     2401 tattgaagtg gtcgaagcct taaaggtcaa tctgatgcgt gtgtttttac gccgtatgga
     2461 cctgcgccaa catcgcaaga tgatcatgat cgataattac atcgcgtaca ccggcagcat
     2521 gaatatggtc gatcctcgct acttcaaaca agatgcgggc gtagggcaat ggattgatct
     2581 gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg attatttatt cctgcgactg
     2641 ggagattgaa accggaaaac gtattctgcc gccaccacca gatgtcaata ttatgccgtt
     2701 tgaacaggcc agtggtcaca ccattcacac aattgcttct ggccccggct ttccggaaga
     2761 tctcattcac caggcattat tgactgcggc ttattcggcg catgaatatt tgatcatgac
     2821 cacgccctac tttgtgccaa gcgatgattt acttcatgcg atttgcacgg cggcgcagcg
     2881 cggggtggat gtcagtatta tccttccgcg aaaaaatgac tcgatgctgg tcggctgggc
     2941 cagtcgcgca ttctttacgg aactgcttgc tgctggggtt aaaatttatc agtttgaagg
     3001 cgggttactg cataccaaga gcgtgctggt cgatggcgaa ctaagtctgg ttggcaccgt
     3061 taaccttgat atgcgtagtc tgtggctgaa tttcgagatt accctggcaa tcgacgataa
     3121 aggttttggt gcagaccttg ccgccgttca ggacgattat atttcgcgtt cacgtctgct
     3181 cgatgcccgt ttatggctaa aacgtccatt atggcaacgt gtcgccgagc gactgtttta
     3241 cttcttcagc ccgttgctgt aaaacgtcgc caacagaggt taaacaggta gtgactatgg
     3301 atatggatct aaacaatcgc ctgactgaag atgaaacgct taagcaggcc tacgatattt
     3361 ttctcgaact ggctgccgac aatctcgatc cagctgatgt tctgctgttc aatcttcagt
     3421 ttgaagagcg cggcggcgcg gagttattcg atccggcgga agactggcag gaacatgttg
     3481 atttcgattt gaacccggac ttttttgccg aagtggtgat tggtctggcg gacagtgaag
     3541 atggtgaaat caacgatgtt ttcgcccgca ttctgttatg ccgcgaaaaa gatcacaaac
     3601 tttgccatat catctggcgg gaataaataa tcgcttttag cgtgaaaaag ggctgacaac
     3661 tgtcagccct tattgttttt aaagcggatc gactttcagg caagaaacgg cgtgtctgaa
     3721 actcccctcc agaacaggac gtgttttggc gcactccgga ccggcaatcg ggcaacgggt
     3781 acggaaaaca caaccggaag gcgggttgat cggcgacggt aattcccctt ccagtaactg
     3841 gatggttttg ttcttctcca gatccggatc aggtatgggg actgccgaca tcaatgccct
     3901 ggtgtaagga tgtagtggat tgtggtagac ctcatcatag gtccccagtt ctactgcatg
     3961 gccgagatac atcaccaaca cacgatcgga aatgtgtttt accacggcca ggtcatgagc
     4021 aataaaaatt aatgacaatc ccatctcacg ttgcagctgc tggagcaggt tgaccacctg
     4081 cgcctgaatt gacacgtcca gcgccgaaac cggctcatcg cagataatca gcttcggttc
     4141 aagaataaga gcacgagcaa tcccgatacg ctggcactgc ccaccggaga actcatgcgg
     4201 atagcggtta atcaggttag gcaataaccc gactttcagc atcatcgcct tcacgcgctc
     4261 gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc agtggttcag cgatgatctc
     4321 gccgatggtc atacgcgggt ttagcgatgc caacggatcc tggaaaatca tctgaatatc
     4381 actgcgaacg gcacgccatt catcgggctt catgcccagc aactctttac ctaaccaggc
     4441 aacatgaccg tcggtcgcct tgaccaaacc gatgatggcg cgagcaaagg tggacttacc
     4501 gcatcccgat tcccctacca cacctaatgt ttccccttca tacaggcgaa gagtgacacc
     4561 atcgacggct ttgagcgttt tcggcggttg ccagaaccac tgtttgccat ctttgatttc
     4621 aaagtgcact ttaagatcgg cgatttcaag gaggactttt cttccttcag ttacagcatt
     4681 cataacagtt cctccaccgg tttaaagcaa gcacgcagac ggccaggcgt aaactcttcc
     4741 agcggcggtg cgctactaca aatttccatc gcatgtggac aacgtggctg gaacgggcaa
     4801 cctttcggta atcgcagcaa gtttggcgga ttacccggga tggtcaacat tgtttcacct
     4861 tccgcatcaa gacgcggcac cgcgttgagc aaaccgatag aataaggatg aacgggttga
     4921 taaaagacat cgcgcgcatt gccatattcc atcgtgcgcc cggcgtacat taccagcact
     4981 ttgtcacaga tccccgccac cacgccaaga tcgtgggtga tcataatgat ggcagtatta
     5041 aattcccgct tcagttcatt caatagcgtc atgatctgcg cctgtacggt gacgtccagc
     5101 gctgtagtgg gttcatccgc aatcagcaac ttaggtcgac atagcaatgc catcgcaatc
     5161 atgactcgct gacgcatgcc gccagaaaat tcgtgcgggt acattttcat gcgtttacgt
     5221 gcttccggca tttttaccgc atcgagcatc cgcaccgact cttcaaacgc ctcagctttg
     5281 ctcatgttct tatgcagcat cagcacttcc atcaactgct caccgacgcg catataggga
     5341 ttcaacgaag tcattgggtc ctgaaaaatc attgagattt gttcagcgcg cagtttattg
     5401 agttcacgct ctggcaaatt gaggatttca cgcccattga aggttgccgc tccgccaatg
     5461 cgtccgttgg cagccaacag gcccatcaac gcaaatgcag tttgcgattt acccgaacca
     5521 gactcaccta caatgcccag cgtttctccg gcacgtaggg aaaaatttaa atcattgacc
     5581 gccgtgacgt cgccgtccgg ggtactaaag gtgacacgca aatctttcac gttcagcagt
     5641 gcgtcagcct gttgttgtgc gagcggcaca gttgcagttt caattacgct catggctgca
     5701 ctccttaacg atctttcggg tcgagggcat cacgcaagcc atcgccgata aagttgaaac
     5761 aaaacagtgt caccacaagg aatcccgctg ggaacaacag taaccatgga gagacttcca
     5821 tcgagttcgc gccatcactc agcaatgccc cccagctgct taacggctct tgcgtaccca
     5881 accccaggaa gctaaggaaa gattcaaaga ggatcatgct gggcaccagt agcgatgcgt
     5941 agaccaccac cacaccgagt acgttcggca caatgtggcg aataacaatg cccggcgtcg
     6001 atacaccgcc aacttgtgcc gcctcaataa actctttgcg cttcagactc agggtttgcc
     6061 cacgcacaat acgagccata tccagccagg aaaccatgcc aatcgccacg aaaatcagca
     6121 ggatgttttg gccgaaaaag gtcaccagca aaatgacgaa gaacatgaat gggaaggagt
     6181 tgaggatttc cagcagacgc atcattaccg aatccacttt accgcccaga taaccggaaa
     6241 gcgaaccata aagtgtcccc acgaccactg ccaccagtgc cgcagcaaca ccgaccatga
     6301 gtgagatacg cccgccaatc gcaacgcgca caagcaggtc gcgaccggat gagtcagtac
     6361 caaagtagtg accggactcc atatccgggg cgctggacat catcgcccag tcagtatcgt
     6421 cataggcaaa ctgggaaagc atcggtgcca ggattacaaa taacgcgatc agcaccagca
     6481 ctatcagact ggcaaccgcc gcacggttat gcataaaacg tcgacgtgcg tcctgccaca
     6541 agctgcgccc ttcgacctcc agcttttcac tgaaattttc cagcgtctcg ctgtttttct
     6601 tacttaacat catcgcgagc tccagtatca gtaacggatt ttcgggtcga taaccgcata
     6661 tagcacatcg acaatggcat taaacaaaat ggttaacgca ccaaccagga tggtcaggct
     6721 taacactaag gaatagtcac ggttcaatgc gccattaacg aacagttgcc caatccccgg
     6781 caaaccataa atggtttcga tgaccataga accggtaata atgccgacaa atgcggggcc
     6841 catataggag agcacgggta acagagcagg ttttaatgcg tggcgtaaaa tgatccgccg
     6901 cataggtaac cctttcgccc gggcagtacg aataaagttg gagtgtaata cttcaatcat
     6961 agagccacgg gtaatacgcg caatactggc gatataggcg agtgacaacg ccaccatcgg
     7021 caatatcatg aatttaagcg ccccaccatt ccagccaccg cccggcagcc aatgcaaaat
     7081 gatcgcaaat atcatgacta ataatggcgc aaccacaaaa ctggggataa caaccccggt
     7141 cattgccagc cccatcacgg tatagtccca tttggtgttt tgttttaatg cggcaataac
     7201 gccagcacta acacccagta ttaccgcaag gaaaaatgcg gcggctccca gtttggcaga
     7261 aacgggaaaa ctggatgcca ccaggtcatt gaccgaataa tctttatatt taaacgatgg
     7321 accgaaatcg ccatgcgcca gttgtttcag gtagctgaaa tactgtgtca tgatcggatc
     7381 attaagatga tatttcgctt cgatattggc catcacttct ggcggtaaag tacgttcgcc
     7441 ggtaaaaggg cttcccggcg cgaggcgcat cataaagaac gaaatagtaa taagaataaa
     7501 tagcgtcgga atcgcttcca gacagcgacg tagaataaat tttaacattg ccctaccttc
     7561 tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg tggagcagga cactcctgcc
     7621 ccacgtattg ccattagtgc ttcacaatgt acatattccg ggtataggta ttatccagcg
     7681 gatctttgcc ggtatagcca ccaacccacg gtttcaccag acgcgcattc acgtagtaat
     7741 aaacaggaac aatggccgaa tccttatcca gctgttgttc tgctttagtg tacagagctg
     7801 tgcgctgcgc ctcgtcagtc actttcagcg tttccgccat aatgctgtca aaggccgggc
     7861 tcttataatg cgcggtattc atcgagctgt tcgacagcat ggtgttcagg aaggaagttg
     7921 gttcgttgta gtctgcacac cagcctgcac gggccacatc aaaagtgccc tggtgacggg
     7981 tgtcgaggaa cgttttccac tcctggttaa ccagtttgac gtttacacca atgtttttct
     8041 tccacaatga agaggcagca atcgccagct ttttatgcag atcggaggtg ttataccgca
     8101 ggttgatggt caacggtttg tctgcggtat aacccgcttc agccagcagt tttttcgctt
     8161 cttcgttacg tttttcctgg ctccagccaa accattctgg ctgagtcaat tttgcgccat
     8221 cagtatacgg tggagtgtaa ccataggcgg gcatgtcgcc ctgcgctttc actttattaa
     8281 caatgatatc gcggtccata cctagtttca gtgcggtacg cacacgcaca tcgttgaatg
     8341 gcggtttctg gttgttaatt tcgtaatagt aagtgcacag gtatggatca acgtgaactt
     8401 cgtccgggat ctcttttttc agcttctgga acaattcgat cggcatgttg ttatacgtca
     8461 tgtcgatttc accactacgg tagcggttga catcggtaac ttcagaagca ataggcaaat
     8521 aggttacctg gttaataacg gttttcgcgt tgttccagta ggtcgggctg cgttcaagaa
     8581 cgattcgttc gtttacgacc caatctttta aggtataggc accgttggtg acgatattac
     8641 caggctgggt ccatttttcg ccgaatttct cgatagcggc ttttggcacc ggtgaagttg
     8701 atgggtgaac aagtaattta tagaagtacg gaacgggttc acttaaggtg acttctaatg
     8761 tgtgatcatc aatagctttc acgccgagat cggtaatcgg ttttttccct tcaagaattt
     8821 catcaatacc ggcgatatgc ccatattgca gataactggc atacggagaa gcagtgttcg
     8881 gatcaacaga acgttgccag ctatacacaa agtcttgtgc tgtgactggc gtgccatcag
     8941 accatttcgc atctttacgc aaatggaagg tccagacttt cgcgtcttta ttatcccagg
     9001 attcagcgac gccaggtgct ggatgaccgt caagatcgct gaccagtaag ccttcaaaca
     9061 ggtctcggct gatattagac tccggaacac cttcaatttt gtgcggatct aatgactgaa
     9121 cttctgaacc attgttacgt accagtgttt gtttttccgc cagtgtgacg cctgcgggta
     9181 catcagctgc cagcgcgaca ttccctgcca ttagcgcagc cagaacgcca gctgctacta
     9241 aacttctctt ggtgatgttg gtcattgttt tttggactcc ctcattataa ttactggtat
     9301 taaccagcat gtgtaatccc ggattgggac cctgcacagg ctcggagact ttctgctgtc
     9361 aggttacttt atcgttacgt attactatca ccgactttat ttattctggt gattcagacg
     9421 atcaccttat gtcgattcgt actgtgtctc ccctgtcgag acacattatt ctttcgcgac
     9481 gctctttaat tgttaattat tctcattacc aataaagaat cgtctggcgg ttgcccggaa
     9541 agtaccaaat gcggttctta ctcgccaata cattttgcaa aattgttaag cgattctctt
     9601 ttattgaccg tatcagaatc tatagcttgc atccatgcct taacaaacat ctttcaattc
     9661 aatcaaatag agattggcta agcatgtcta tcttttctga ttacagttcc agtagtgaaa
     9721 tgcacaataa tttaacaatt gattattatt tagcactttc ctctaccaaa ggtagtggaa
     9781 ttactaatat catttcaatt attcttcagc aagcccagga ttatgatgtg gcgaaaataa
     9841 catgatggtg aggagaagca gttaaggaaa tccttataac taactgtaat ttgagaagaa
     9901 tatttttacc tattcctttt gcttttgata actgcgcggg aacaaagtaa aaataatgac
     9961 gaagtaaacg gagcatggca gctccgtttc atttgaaagg aattaattaa gcaggccggg
    10021 gaaaatcccc ttaataccag taacgataaa ttcaatcccc aatgccatca gcaatagccc
    10081 cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa acccgtacca gccacggtgc
    10141 catgcggaac aatccccagc aacataaagc gaacaatgca atagccacaa agaaaccaaa
    10201 cagatagcta atgctgtgat aacgcgtacc ccagacgatg gtagaactga tcgcccctgg
    10261 ccccgccatc aacggcaacg ccaatggcac cacaccaata ctttcacgta ccgcggtttc
    10321 tgatttttct tgcttgttct gtttatcctc gccaagcttg ccgctgatca tcgacatcgc
    10381 tatcgtcacc accaggatgc ccccggcgat acggaacgaa tcaattgata taccaaaaag
    10441 ttgtagaatc gtgtcgccga gaaaaagcga gatccacaag ataatggcca cagacaggtt
    10501 ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga taactggtca tgctgataaa
    10561 gacgggaata atccctaccg ggttgaccag cgcaaataac ccgatgaaaa atttgaagta
    10621 aacgggaaaa tcaaaaaggg tctgaatcac ggttagctcc gaagcaaaag ccggataatg
    10681 ttagccataa ataaggttga aaagacgcgc tgacaatacg ccttttgaca gcatttttca
    10741 cctcctaact acttaaaatt gctatcattc gttattgtta tctagttgtg caaaacatgc
    10801 taatgtagcc accaaatcat actacaattt attaactgtt agctataatg gcgaaaagcg
    10861 atgctgaaag gtgtcagctt tgcaaaaatt tgatttggat cacgtaatca gtacccagaa
    10921 gtgagtaatc ttgcttacgc cacctggaag tgacgcatta gagataataa ctctaatgtt
    10981 taaactcttt tagtaaatca cagtgagtgt gagcgcgaat aagcttttga ttttcatcgg
    11041 ttaagcaaat catcaccgca ctgactatac tctcgtattc gagcagatga tttactaaaa
    11101 aagtttaaca ttatcaggag agcattatgg ctgttactaa tgtcgctgaa cttaacgcac
    11161 tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa gagcaagtag
    11221 acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa
    11281 tggccgttgc cgaatccggc atgggtatcg tcgaagataa agtgatcaaa aaccactttg
    11341 cttctgaata tatctacaac gcctataaag atgaaaaaac ctgtggtgtt ctgtctgaag
    11401 acgacacttt tggtaccatc actatcgctg aaccaatcgg tattatttgc ggtatcgttc
    11461 cgaccactaa cccgacttca actgctatct tcaaatcgct gatcagcctg aagacccgta
    11521 acgccattat cttctccccg cacccgcgtg caaaagatgc aaccaacaaa gcggctgata
    11581 tcgttctgca ggctgctatc gctgccggtg ctccgaaaga tctgatcggc tggatcgatc
    11641 aaccttctgt tgaactgtct aacgcactga tgcaccaccc agacatcaac ctgatcctcg
    11701 cgactggtgg tccgggcatg gttaaagccg catacagctc cggtaaacca gctatcggtg
    11761 taggcgcggg taacactcca gttgttattg atgaaactgc tgatatcaaa cgtgcagttg
    11821 catctgtact gatgtccaaa accttcgaca acggcgtaat ctgtgcttct gaacagtctg
    11881 ttgttgttgt tgactctgtt tatgacgcag tacgtgaacg tttcgcaacc cacggcggct
    11941 atctgttgca gggtaaagag ctgaaagctg ttcaggatgt tatcctgaaa aatggtgcgc
    12001 tgaacgcggc tatcgttggt cagccagcct ataaaattgc tgaactggca ggcttctctg
    12061 taccagaaaa caccaagatt ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt
    12121 tcgcacatga aaaactgtcc ccgactctgg caatgtaccg cgctaaagat ttcgaagacg
    12181 cggtagaaaa agcagagaaa ctggttgcta tgggcggtat cggtcatacc tcttgcctgt
    12241 acactgacca ggataaccaa ccggctcgcg tttcttactt cggtcagaaa atgaaaacag
    12301 ctcgtatcct gattaacacc ccggcttctc agggtggtat cggtgacctg tataacttca
    12361 aactcgcacc ttccctgact ctgggttgtg gttcctgggg tggtaactcc atctctgaaa
    12421 acgttggtcc gaaacacctg atcaacaaga aaaccgttgc taagcgagct gaaaacatgt
    12481 tgtggcacaa acttccgaaa tctatctact tccgccgtgg ctccctgcca atcgcgctgg
    12541 atgaagtgat tactgatggc cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca
    12601 acaatggtta tgctgatcag atcacttccg tactgaaagc agcaggcgtt gaaactgaag
    12661 tcttcttcga agtagaagcg gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg
    12721 caaactcctt caaaccagac gtgattatcg cgctgggtgg tggttccccg atggacgccg
    12781 cgaagatcat gtgggttatg tacgaacatc cggaaactca cttcgaagag ctggcgctgc
    12841 gctttatgga tatccgtaaa cgtatctaca agttcccgaa aatgggcgtg aaagcgaaaa
    12901 tgatcgctgt caccaccact tctggtacag gttctgaagt cactccgttt gcggttgtaa
    12961 ctgacgacgc tactggtcag aaatacccgc tggcggacta tgcgctgacc ccggatatgg
    13021 cgattgtcga cgccaacctg gttatggaca tgccgaagtc cctgtgtgct ttcggtggtc
    13081 tggacgcagt aactcacgcc atggaagctt atgtttctgt actggcatct gagttctctg
    13141 atggtcaggc tctgcaggca ctgaaactgc tgaaagaata tctgccagcg tcctaccacg
    13201 aagggtctaa aaatccggta gcgcgtgaac gtgttcacag tgcagcaact atcgcgggta
    13261 tcgcatttgc gaacgccttc ctgggggtat gtcactcaat ggcgcacaaa ctgggttccc
    13321 agttccatat tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca
    13381 atgcgaacga caacccgacc aagcagactg cattcagcca gtatgaccgt ccgcaggctc
    13441 gccgtcgtta tgct
//
LOCUS       DAJTRQ010000108        13429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000108 DAJTRQ010000000
VERSION     DAJTRQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..343
                     /locus_tag="OO332_003142"
     CDS             <1..343
                     /locus_tag="OO332_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5943527.1"
                     /translation="ECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDN
                     LLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLR
                     ETIKNLYASMA"
     gene            500..1249
                     /locus_tag="OO332_003143"
     CDS             500..1249
                     /locus_tag="OO332_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5943528.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1271..1837
                     /gene="yieF"
                     /locus_tag="OO332_003144"
                     /gene_synonym="chrR"
     CDS             1271..1837
                     /gene="yieF"
                     /locus_tag="OO332_003144"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5943529.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1891..3228)
                     /gene="adeP"
                     /locus_tag="OO332_003145"
     CDS             complement(1891..3228)
                     /gene="adeP"
                     /locus_tag="OO332_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5943530.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3393..4058
                     /gene="yieH"
                     /locus_tag="OO332_003146"
     CDS             3393..4058
                     /gene="yieH"
                     /locus_tag="OO332_003146"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5943531.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4125..4598
                     /locus_tag="OO332_003147"
     CDS             4125..4598
                     /locus_tag="OO332_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR5943532.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4647..5234
                     /locus_tag="OO332_003148"
     CDS             4647..5234
                     /locus_tag="OO332_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5943533.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5296..6018)
                     /locus_tag="OO332_003149"
     CDS             complement(5296..6018)
                     /locus_tag="OO332_003149"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5943534.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6020..7195)
                     /locus_tag="OO332_003150"
     CDS             complement(6020..7195)
                     /locus_tag="OO332_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5943535.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7222..8838)
                     /gene="bglH"
                     /locus_tag="OO332_003151"
     CDS             complement(7222..8838)
                     /gene="bglH"
                     /locus_tag="OO332_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5943536.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8924..10321)
                     /gene="bglB"
                     /locus_tag="OO332_003152"
     CDS             complement(8924..10321)
                     /gene="bglB"
                     /locus_tag="OO332_003152"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5943537.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10337..12214)
                     /gene="bglF"
                     /locus_tag="OO332_003153"
     CDS             complement(10337..12214)
                     /gene="bglF"
                     /locus_tag="OO332_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5943538.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12348..13184)
                     /gene="bglG"
                     /locus_tag="OO332_003154"
     CDS             complement(12348..13184)
                     /gene="bglG"
                     /locus_tag="OO332_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5943539.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3544 a         3372 c         3078 g         3435 t
ORIGIN      
        1 ggagtgtccg ttaaactcag gctacctcag ggcgctggac aatgtgttac aggagctggg
       61 gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt ttatggcagc
      121 gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact
      181 ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct
      241 ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg
      301 gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg
      361 cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt
      421 tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc ataatggacc
      481 attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca
      541 tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca
      601 tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta
      661 tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga
      721 taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac
      781 aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag
      841 cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca
      901 aaacgatcct aacgaagcgc gggcgcagct cattacgctg cgccgaagcg tgctaaaact
      961 aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg
     1021 cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt
     1081 gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa
     1141 acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat
     1201 gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac
     1261 aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg
     1321 ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt
     1381 caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga
     1441 aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt
     1501 gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg
     1561 gctttcccgc ctgccggatc aaccgctggc cggtaaaccg gtgttgattc agaccagctc
     1621 aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct
     1681 cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga
     1741 tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac
     1801 cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat
     1861 gcatggcggg tttttaatgc gctatcgatt ttaatgagcg tcgataaata caatcttcag
     1921 gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc
     1981 aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct
     2041 gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt
     2101 cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc cagctgcagc
     2161 gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga acagcagacc
     2221 aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag aggactcaat
     2281 ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga tactgtcgac
     2341 atacagcgcc tgcttcatgc gcgggaattt ccccttctca tccgccagac ctgctttatc
     2401 ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accaacatga aagagaaaat
     2461 cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca caactgtcat
     2521 tacgctcggc ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca tccagcccag
     2581 cagcgtcgtc accacgatag aaaccagcac cgctgcgtga atgttgcgcg aggccagaat
     2641 agcaatgatg aaaaagccga ggatacccag aagtacgctg tgagaagtca gattaccgat
     2701 gctcaccagc gtttccgagt tagcgacaat cacacctgcg tttttcagcc ccatcatgcc
     2761 gataaacagg ccgataccgc tggtaatacc cacacgcaga cttaccggaa tgttagcaat
     2821 catccagtaa cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc cccagaagat
     2881 tgcgcccata ccgatctgcc acggcagccc cattgcctgg acaaccacaa aagcgaaaaa
     2941 ggcgttaagc cccatcgcag gagccagcgc caccggaagg tttgcaaaca atcccatcat
     3001 aatgctaccg aaagcagcaa tcagacaggt tgttacgaag acggcactgg tgtccatgcc
     3061 agcaacgccc agaatttgtg ggttaacaaa tacgatgtag accatcgtca ggaaggtggt
     3121 gaacccggca atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt taaaaacgcg
     3181 ttccagcatc ctctgaccag aagtctgggt ggtatgttga tgactcatta tctatttccg
     3241 aacaaggagg gaaaatccgt cgctatcgta taccaaaatg cgacaatagg cgcgtttgtg
     3301 agagactttt ttattggatt tacttatacg gcaacgattg cgttgcgcaa atcggctcta
     3361 cgaaaacgtt aaactgatta aaaaggaaag gcatgtcccg gatagaagcg gtatttttcg
     3421 actgcgacgg tacgctggtc gacagtgaag tcatttgctc ccgcgcatat gtaacgatgt
     3481 ttcaggaatt tggtattacg ctcgatcctg aagaggtatt caaacgtttc aaaggtgtaa
     3541 aactgtacga aattatcgat attgtttccc ttgaacatgg tgttacgtta gcgaaaacag
     3601 aagctgaaca cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa ctggaagcca
     3661 tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg gtatctaacg
     3721 gcccaaataa caaaatgcag cattctatgg gcaagctgaa tatgttgcac tacttcccgg
     3781 ataaactgtt cagcggctac gatattcagc gctggaagcc agacccggcg ttaatgttcc
     3841 atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat tctggttgat gactcagttg
     3901 ccggtgcgca atctggtatc gacgcaggta tggaagtgtt ctacttctgc gccgacccgc
     3961 acaataagcc gatcgttcac ccgaaagtca ccacctttac ccatctttcg cagttaccgg
     4021 aactgtggaa agcgcgtggt tgggatatta cggcatagtt cttcacactc ccttcactta
     4081 ccccgcttaa attggcgctc aaaggtaagt aaagggagtt tgatatgtct gtttcacgtc
     4141 gggtaataca tcacggactt tattttgcgg ttttaggacc gttaattggt gttctgtttc
     4201 ttgtcctcta catattcttc gcaaaagaac cgctggttct tttggtaata atacaggtac
     4261 ttccattgtt tctcttattg tcgattacta cgggagctat tcctgcattg ttaaccggcg
     4321 tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt tgtctggctg
     4381 gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc agttattgta catattaagg
     4441 gcatggcttc ctcggagttg tttgaaaaca ttctttctgg tgacagtctc gttgtccgca
     4501 tcattcctgc attgctggca ggtgtggtga tgagcagaat cattacccgt ctgcccggat
     4561 tggatatttc atgtcctgaa acagactctt taagttaagc gggatacttt atctttgggc
     4621 tactcaaaag cagacaggat gttactatga ctcaaaatat caggccgtta ccccaattca
     4681 aatatcatcc caagccactg gaaacaggcg catttgaaca ggataaaacc gtagagtgcg
     4741 attgctgtga acaacagacg tcagtttatt actcgggtcc cttttattgc gttgatgaag
     4801 ttgaacatct ctgtccgttg tgtattgcgg acggttctgc tgctgaaaag tttgcaggta
     4861 gttttcagga tgatgccagc atagaaggtg ttgaatttga gtatgatgaa gaggacgaat
     4921 ttgccggtat taagaacaca tatcctgatg aaatgctgaa agagttggtt gaacgcacgc
     4981 caggttatca tggatggcaa caggaattct ggcttgcgca ttgtggcgat ttctgtgctt
     5041 ttatcggcta tgtgggctgg aatgatataa aagatcgcct cgatgaattt gccaaccttg
     5101 aagaagattg tgagaatttc ggtattagaa attctgatct agctaaatgc ctgcaaaagg
     5161 gtggtgattg tcagggttat ctcttccgct gtctccactg cggcaagctg agactgtggg
     5221 gtgatttttc gtagttattt aaataatgag aacaggccgg agcgtaattc atacatccgg
     5281 ccttatttct tcagcttaac cgaacgctaa ttctgcggca gcggctttat cagcgattac
     5341 catcagcgaa ggatgcagct gcaacacgga agcaggaaca tcttcggtaa ccggaccttg
     5401 cagcatattt ttcagtgcct gcgctttacc tgcgccactg acaattatca gcaagttttt
     5461 cgccgccatg atgcttttcg gccccatcgt cacgtagctg tcaggcacta acgaaaagtc
     5521 gccgcccaac tcaccatgcg ccacaatatc aaccatctct cccaggatcg ggaactccac
     5581 ggtttgctca tggaaatggg tcgtattcgg caaattgcca caaaaatgac catctgcacc
     5641 taatcccagc accaccaaat ccaggccgcc ttcacgcgcc agtttctgat catgctcgcg
     5701 gtagttatca atagtgagct tctggatatt ctcttctttg atccccgcag gggtgaaaaa
     5761 cagattacgc agattggtaa tcgttacgcc ttctccctct ttgccgcgaa agggaatttc
     5821 atcgaaatta tagaaatagc agttatcgta ccaaggctta cctttaacca gggtggtgag
     5881 gtattcatac atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa cacgacgcgt
     5941 cttcgacata taaccaagca gatggtgtgc cgcgacacgg ctcatttcct ggtaatcttc
     6001 ggtaatgatt aatttcatct taatgcctta ttttaaattt ctgtacgaag gctgcatagg
     6061 caggccgcca gacatccatt tcatggttca ggccgggata ttcctggtaa tcaaacttaa
     6121 tttttttctg ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt
     6181 tatcaccaac gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca
     6241 gccgggccgc gacaccttca tccggtacgg ttgtcgtggt aacaccactg aatgtggcca
     6301 gccagccaaa gctttccaga tgattcattc cggaaaccag cgcctggtac ccgccttgtg
     6361 aaagccctgc cagcgcgcga ccatcggcat ctttacggac actaaaacgc ttgctaatca
     6421 gcgggataat atcgttcatc agttcgcgat ctgccgcttt agcattcagc agataaaaga
     6481 ctttacgtct ttcctgaggc acgaaatctt cgggaataat gcccttcgca tcggtttcgg
     6541 tatccgggat caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga
     6601 tttgcgggat acgcccctga tcgatagcgg aacgtccagt atcaccaaaa ccgtgataga
     6661 aatagagcac tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc cagacataca
     6721 tctgacgttc agattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgcg
     6781 caacagaacg cgtatccaga taactgccta gcaccagaat catactggag ttaacctggc
     6841 gctgaggctt agtcattgcg gtgcctgtat caatgctgcg tacaccatca acattgaaaa
     6901 aatactcgta caggttgcct ttcaggatag gtgtgcgcca cgaccagact cctgcttcgt
     6961 ctttggtcat cgggtgaata ttgtccggaa ccggaacacc caccactaca gagacatttt
     7021 ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc tgagtcacat
     7081 actgtttcac cgggatagtg ggtgccgggc tggctggcat atccgctgct atggcccact
     7141 gagcagaaat accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa
     7201 tattcctttc attaagttcc attaccacca gatttcagcc tgggcaccga cagcaaactg
     7261 gtcgtcttta ttgtcttcga aggtgaagcc atccagttcg ttttccaggg ctttgatata
     7321 cgtggcgtag aaacgaattt ccagacgcga ggtcaacata ctggtattga ctttaaaggt
     7381 atggaagagc gtggttttat aaccagactc attaaattta ttactattcg catctttgtt
     7441 ttgctgggtg aaatagccca gttcaacacc tgtctggtta tattggtccc aaatataggc
     7501 cgggcgaaca accgcacgaa tagattcgaa atcagagtgt gcgcctgttt cgtagctata
     7561 aatatcgtta ccgaaggagt aaacaatcgc gttagccaca ataaaatgat cgccgatata
     7621 ggcttcgccc tgcgaggtca gacgaactgc tgttccgccg gtgtgatcac cataataacg
     7681 accattaaag gtggtaaatg gacttgcgcc agcataacgg ccaaagttcc tggcgataga
     7741 gttattcgca accaggaagg agaattcgtt gaagccacct ttatcaaatt tctgcgttaa
     7801 agatgtgccg aacatccagg tatctttcca gtcataatac ccgttattat cctgattatc
     7861 tttttccgat gcgctttcgt ttgccgtgac ataacgacca ctcaccatta aggtcgcttt
     7921 atcccataac gggatatctt tatagcgtaa atcaatagta tgggtattaa tctgctgttt
     7981 gttttgcagg ctgcgatcgt aatcatcgac atcttcgcga accagcgcga tatcaatttt
     8041 acccggaccg actttccagt tttccagacc tacacccgct gcggcatcag tacgctgcgt
     8101 tttccagtcc agcatctgga tttcaatttt cggcgcaccg tgtttaccca cccagaaatc
     8161 agcctctggc gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg agaactgcat
     8221 aaagttctcg ccaccggcgt tatcgccaaa ccagccggta gagtactgct gaccaacgtt
     8281 accatccatc atcacaacgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt
     8341 taactgcaaa tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaatcgc
     8401 ccatgattta ggtgaaccat gagaggcggt cccccaacca gaacggtaat agccgttgta
     8461 actaaagcca atttcatctt tcacaaactt actgaaatct ttcagagtca tggaagaata
     8521 aatcgagcct gtctcactgg catttttctc ttcatttttg accagtacag catcaggttt
     8581 tgcggcacta ctggtcggga acggattggc ggcataccct tgatcattcg tacttacgct
     8641 acgattcacc gtcgctggcg tatatttttt cttctcttca tctttatact ttttgagttc
     8701 gctttgcgtt tctcttaacg ctttttctaa taactcaagg cgttgttcca ccgttaatga
     8761 ttcagccaat gattgtgcag aaaaggctaa cggtgccatt aataagatgg cagaggtaat
     8821 aagatttcgt ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgtatcgcat
     8881 ccagactgtt ctgaatgcga cgataattaa ggtgctttat tggttatttt tttaatgaca
     8941 gcccccgcgt tttgatcact tctgcatacc atccgaagct tttcttacgc gttcttgtga
     9001 ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt
     9061 gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca
     9121 caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag gcaattcgat
     9181 aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tacgcctaat ccattctcga
     9241 caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc cgtaatccaa
     9301 ccggatcaat ttgccacccc ctctctgaac ttttcagatg cggattgggg atcatattca
     9361 gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt
     9421 aataactaaa agagatgaaa tctacagtat gttttaaatc ttctgcgtca ctttcagtca
     9481 tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga tactggccac
     9541 gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat
     9601 cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccgattttcg
     9661 cttcggggat caggctatga taggctttaa ctgcccgcgc actggcaacc agttgatgat
     9721 ggatcgcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg
     9781 gcgcgtgtaa cgacatattg atttcattaa acgtcagcca taacgctact ttatgttggt
     9841 agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc cgattagccc
     9901 aaccgccgta gtttttcacc agcccatata gcatttcgta atgggataac gttaccagcg
     9961 gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt
    10021 cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca
    10081 gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat
    10141 cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta
    10201 cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc atcttccagc caggcacctt
    10261 ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcgtaa
    10321 ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg cttatctgcg
    10381 ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt actgattaat accggcgtcg
    10441 tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg
    10501 tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg
    10561 tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg ccaatggcgt
    10621 gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg
    10681 agggcagaat ggcgatacct ttacccagca ggccactggc aaacgtggta tcagcgacgt
    10741 gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg
    10801 taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttactggtga
    10861 tgaaatgaag catcaacgta ccaacaaatg cgcaacctat ggcaatgaca ccgccaataa
    10921 cgctggccca gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg aaggtgaaaa
    10981 tacttggcaa accaaaggag taaactttcg tttgcgcgta gccaatgatg gtggccccca
    11041 atgctccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg acgccatata
    11101 ccgctggttc ggtgatacca aacagacccg tcaacgccgc tgatcccgcc accacttttt
    11161 tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg
    11221 cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca
    11281 gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta
    11341 ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcattt
    11401 cgcttatcca ggttgatagc ggccccacca gcagaaaggt gacgggtgtg ataaccatca
    11461 gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc
    11521 gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat
    11581 tcaacaatgt gaccggaata cccaggaaat ccagccccgg cgcatccgct ttttgcccgt
    11641 tctcgaaggc agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa
    11701 atggattacc gccgaagcgt ttgcccgcgg tgtatcccag gattatcggg aagaacccaa
    11761 acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact
    11821 gaaaagtgag cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcagaccga
    11881 tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct
    11941 catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttaaccgcca
    12001 ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca
    12061 ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta
    12121 acttaaaacg taatcgcgtt gcacaatgca tcagactcac aatgttatct gcgcccccga
    12181 ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca
    12241 aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg
    12301 ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa taatatttca gtgttctttg
    12361 cgcacgcgct ctatatttat ggctaaaaac ataatctctg cgggtgaaat tttacgttga
    12421 tactgcaaac caataaaaat ggcgatccgt tccgcacatt gccatgcttg cgggtaattt
    12481 tgttttactg cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg ttcaagaata
    12541 cgccaggata aaaacttcag atgtgtaacc agtcgctgat aactcaagct ttcttcctgg
    12601 taattaaggc tgaactgaaa ttttattaat tgcagcattt cgcgcattaa ctgcgtgaca
    12661 cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag
    12721 cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct
    12781 tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc
    12841 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
    12901 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag
    12961 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
    13021 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
    13081 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
    13141 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
    13201 ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
    13261 tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat
    13321 taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc
    13381 gcagttatta actttgtgta attttaggaa tttataaagt tatatataa
//
LOCUS       DAJTRQ010000109        13057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000109 DAJTRQ010000000
VERSION     DAJTRQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..575)
                     /gene="yehW"
                     /locus_tag="OO332_003155"
     CDS             complement(<1..575)
                     /gene="yehW"
                     /locus_tag="OO332_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5943540.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTV"
     gene            complement(580..1506)
                     /gene="yehX"
                     /locus_tag="OO332_003156"
                     /pseudo
     CDS             complement(580..1506)
                     /gene="yehX"
                     /locus_tag="OO332_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(1499..2657)
                     /gene="yehY"
                     /locus_tag="OO332_003157"
                     /pseudo
     CDS             complement(1499..2657)
                     /gene="yehY"
                     /locus_tag="OO332_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(2664..3581)
                     /gene="osmF"
                     /locus_tag="OO332_003158"
                     /pseudo
     CDS             complement(2664..3581)
                     /gene="osmF"
                     /locus_tag="OO332_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(3833..6130)
                     /gene="bglX"
                     /locus_tag="OO332_003159"
     CDS             complement(3833..6130)
                     /gene="bglX"
                     /locus_tag="OO332_003159"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5943541.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            6326..8041
                     /gene="dld"
                     /locus_tag="OO332_003160"
     CDS             6326..8041
                     /gene="dld"
                     /locus_tag="OO332_003160"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5943542.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8079..9011)
                     /gene="pbpG"
                     /locus_tag="OO332_003161"
     CDS             complement(8079..9011)
                     /gene="pbpG"
                     /locus_tag="OO332_003161"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5943543.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9185..9772)
                     /locus_tag="OO332_003162"
     CDS             complement(9185..9772)
                     /locus_tag="OO332_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5943544.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            9942..10520
                     /locus_tag="OO332_003163"
     CDS             9942..10520
                     /locus_tag="OO332_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5943545.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(10637..11398)
                     /locus_tag="OO332_003164"
                     /pseudo
     CDS             complement(10637..11398)
                     /locus_tag="OO332_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(11451..12887)
                     /gene="mdtQ"
                     /locus_tag="OO332_003165"
     CDS             complement(11451..12887)
                     /gene="mdtQ"
                     /locus_tag="OO332_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5943546.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
BASE COUNT         3111 a         3661 c         3302 g         2983 t
ORIGIN      
        1 accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc
       61 agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc tcgactcatt
      121 cccatacctt tcgcaacttc tgtcacgctg ggatcaatcg ctcccagccc ggcaagtgtc
      181 acctgcagga tgggcagcac accgtaaagg atcaaggcga taatcgctgg ttgcagacca
      241 aagccgatca ccggaacggc aatcgccagc actgcgacag gcggaaaagt ctgtccaacg
      301 gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt aacagcaatt
      361 ccggcacctg tgccaatgat caccgcaaac aaactcgaaa ttcccaccag ccagaaatga
      421 gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag ttgtgggaac
      481 aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagcgccac aaaaagagca
      541 atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcca ccagcagatc
      601 ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc accaccggca gcacctcgca
      661 tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc
      721 accttctgcc cgttcttcgc gacgcacgta atccgccacg ctacgtaacg aaagcaggtg
      781 cacccccagt tcactacgtc caaaaaactg gcggacaaaa tcattagccg gacgagtcag
      841 catcgtcagc ggattcccct gctgcaccac ttcaccgtga tccatcaata ccagatgttc
      901 tgccagccgt agcgcctcat caatatcatg agtgaccagc acaatagtgc gccccagcaa
      961 acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc
     1021 aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcacgcg ccactcccac
     1081 acgttgctac tgaccaccgg aaagctgatg cggataacgc tcacgcaaat ttgactccag
     1141 ccccagtagc gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg accatttttg
     1201 taattgcggc acggtagcga tgttttgcgc cacgctccag tgggggaaca ggccaataga
     1261 ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc
     1321 tccggcaaag cggatcactc cgctgtcatg ctccaccagg cggttaatca ttttcagggt
     1381 ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt cctgaaaatt
     1441 gagattgaga tcgttaacgg ctttttgtgc gccgaacagt ttgctgacat ggctaaattc
     1501 aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagcgcg tcggtaagca
     1561 ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggcg ctgcttagca
     1621 gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca
     1681 tacctacagt ttgcaccatc accacccgca ggctgcgcag aaataccggt aacgccagcg
     1741 gtaactgaac atgcaggaaa cgctgcgccc cgctcatccc catcgctctg gcgctctcca
     1801 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     1861 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcttcc
     1921 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     1981 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgcgcag
     2041 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     2101 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     2161 attctttcat ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     2221 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     2281 gcatcgctac aggccagcaa tgccagcgcc gccgccagcc agaaaccact gccgaggctg
     2341 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc agctccccac
     2401 accaacaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     2461 gggacaaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     2521 ggccacagtt gccagagatg aagcccttca cccgagacta aacgattagg cgcgtaactg
     2581 ataaacggca gcgccgctgc gattgccgtc agcaacaaca gcagcgccag aacaggatta
     2641 atacgtagat aagtcacggg aaattacttc gcccaccctt tttgtttcag gtagtcggca
     2701 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     2761 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactccttc
     2821 aacaccgatt cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc ttgcggatcg
     2881 cttaaggttt gcagccccag cgccgcgacc ggagcgtcag tgccgtaagc cattgcggca
     2941 ttaacgccag aggtttgctg ggcagcggct ttaatcgtca ccgccgtgtc gccaccagcc
     3001 agtgacagca actgatcctg accgagctta aagccatagg ctttttcaaa cgcgggtaac
     3061 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctcttgc
     3121 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     3181 acggcgatag tccaggtgtt atttgcaggc gcgggcgtca gccagattaa cttgttgtgc
     3241 tccgcatcga gctttttgac tttctcgtag ccctgcccgg catttttcca cgctgcatcg
     3301 ttttcatctt taaagaagaa agcgccattg ccggtatatt ccggatagat atccagttca
     3361 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     3421 ggtacgccgt ggctttcgag tacctgcaag atgatgttgc cgagcagcgc gccttcggta
     3481 tcgatttttg aaccgacttt aacgggggaa gccgcctata gcagcaggct cacggctgcc
     3541 aacataacca gtgaacctgc ccagagcttt aagagtggca tgatgctttc ctcattcttt
     3601 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     3661 gcggaaggat tcagttgcag aatcagataa atacttaaga ggcatattcg gtccggcgtt
     3721 tcctgccgga tgcggcgcga gcgccttatc cggcctacaa agggcgaaaa ttcagcaagt
     3781 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     3841 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaataca ttgaacttgc
     3901 caggctcggc gtcatatttc atctgttgat tccagaactt cagcgcctca atatcgatcg
     3961 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     4021 gtttcacagg gcgactcatg gaagccgtca catcctgcaa gtacatctgc actaccgtgg
     4081 ccccctcgcg cttaccggtg ttcgtcacct gcacgctggc cgtcactttg ccgtcacgct
     4141 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     4201 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     4261 gcttgtcggc attatacgga cgaccggtgt tcagatggct gtagtacacc gggatctgcc
     4321 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     4381 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     4441 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     4501 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gccttgcgga atagtgatat
     4561 cggtccggct ggaggcttcg tgcgccatcc cctgtgcttc accgactaca gccaccacca
     4621 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gaacgcggat
     4681 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     4741 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggaattt ttaatcccgg
     4801 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     4861 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     4921 tttcgagacg gtttttcagc aacactaggc tttcgcgcgc cacttcacgc gcttctttac
     4981 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     5041 tgtatgggtc gttaaacaac cccatatcat atttaacgtt cagtacatgg cgggcagcat
     5101 cgtccagctc tgccatggtc actttgccgg atttaatcaa cccaggcaga tacttcgagt
     5161 agtactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     5221 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     5281 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     5341 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     5401 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     5461 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     5521 tcaccgagta acgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     5581 ttattgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     5641 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     5701 aagcagagac acgcccgacc gttttcaccg catcgaggtt aaaagacgag gccagaccga
     5761 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     5821 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     5881 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     5941 gattatccgg gcctacgctg attaagcgca gctgaccaat tttctcatca accatcattt
     6001 tcttgagcag atcggtgacg aacgcatccc gcgcttcagg cgttaatgga tggttaccga
     6061 ataaatcatc cgccagtgct ggctgcagga ccagactcac cgcgattcct actgaacata
     6121 gccatttcat atggattttt cttcctttgt tgcccgacgt ggcagcgaaa atggtgcaaa
     6181 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atactcagtt tattgtctga
     6241 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     6301 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     6361 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     6421 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     6481 tggctcacta ctggaattgt ggcgggtgct gaaagcctgc gtcaccgccg acaaaattat
     6541 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     6601 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttctcggcaa
     6661 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     6721 accgctggga cgcgaaccgc attcggtgat tggatcatcg tgtataggcg catcggtcat
     6781 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     6841 aatgtcgtta ttcgcgcgta tagatgaaga tggcaaactg acgctggtga accatctggg
     6901 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgacg atcgcatcaa
     6961 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     7021 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatccggatc ggttatttga
     7081 atcttctggt tgcgccggga agctggccgt ctttgcggta cgtcttgata ccttcgaagc
     7141 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     7201 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatgcaccg
     7261 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     7321 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     7381 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgca atgcaaaaat tcggtcacct
     7441 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     7501 gctgttaaaa atggcaggcg atggcgtcgg tgaagccaaa tcgtggctgg tggattattt
     7561 caaacaggcc gagggcgatt tctttgtctg tacgccggag gaaggtagca aagcgttttt
     7621 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     7681 cgaagacatt ctggcactgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     7741 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     7801 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttaaaaga
     7861 acagatgctg gaactgctac agcagcgcgg cgcgcagtac cctgccgagc ataacgtcgg
     7921 tcatttgtat aaagcaccgg agacgttgca gaagttctat cgcgagaacg atccgaccaa
     7981 tagcatgaat ccggggatcg gtaaaaccag taaacggaaa aactggcagg aagtggagta
     8041 aaaattacgg atggcagagt atcgccatcc gaattcactt aatcgttctg tgccgtctgc
     8101 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgccggcaca
     8161 ggcatcactt taccggtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
     8221 tatttgccaa acgcgtccat cactaccagc gccaccggtt tattattgat aaccgtacgc
     8281 atcaccagac aatggcccgc cgcattggta aagccggttt tggttaactg aatattccag
     8341 ttatcgcgat acaccagatg attagtattg cggaacggca gcgtatacgt cgggttagag
     8401 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
     8461 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
     8521 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
     8581 ataaaggctt tgtaaccacc gggataatgg tgcgcaaggc ttgccgccgc gcggttttct
     8641 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
     8701 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
     8761 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
     8821 ggacgcacca gatccgggtg gttcgaatag atcactttgt tggtattcag atcaacaatc
     8881 atcgcgctac cggaggcaat ttccggttgt gaagcggtgg tagcggctgc cgttttcgca
     8941 acggcctgcg gtgcaaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
     9001 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acgggttgcg caccgccgga
     9061 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
     9121 gagaatcgtg agtaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
     9181 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggtg agcagcactt
     9241 ccagtaccag cacgccaatc gccagcgtcg aactggagaa gctaaggcct tcctctttgt
     9301 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
     9361 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
     9421 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagagac ggacgctgcg
     9481 ggtaattgcg cgccatccac cagatgaccc gccccatcac cgcaacccca gccagcataa
     9541 cgccgtaaaa caagacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
     9601 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
     9661 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
     9721 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
     9781 cttgttgtga gttcatgaac ctgacagttc aagtataagc cagcttgtga ttattttttg
     9841 ttcatccatg taatttgcac cgtctgatgt atgtcctttt tcagcgtgaa tgattcacag
     9901 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
     9961 tctcacaata tggttatgcc gcgctggtta tcggtagcct agcggaaggt gaaaccgtga
    10021 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccactg gtggtacttt
    10081 ctgtggcgct tggtggcatg attggcgacc aggtactcta tctgtgcggg cggcggtttg
    10141 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    10201 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    10261 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    10321 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    10381 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    10441 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    10501 agccggataa tcaggcgtaa agcagttgcc acagataagc attcactcca catccggcac
    10561 cgggtgttcc atgcctgatg cgacgctggt gtcttatctg gcctaaccca ttgaaatatc
    10621 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    10681 ccactatcaa cgactgccct gtcgtgtaat ttgcgccctc cgaacaaagc cacaccacca
    10741 ggctggcaat ctcatgcgtt gcgccaaaac gccgtagagg aatcgaaggc tccgcgtcgg
    10801 gcttcacgtc gctatcatcc atgctgttca ttggcgtggc tatcgcccca ggcgcgactg
    10861 cgttcaccaa aatcttatgc ctgaccaact ccagcgccat cgttttggtt aacccgccga
    10921 gcgcatgttt agcggctgtg taggcgctgg catccggcag cggcgtatgt tcatgtaccg
    10981 acgtaatgtt gatgatgcga ccgccctgcc cttatttcac catctgacga gccgcaattt
    11041 gcgagcataa aaatgcacca tcaacatcaa cggtaaaaat cttgcgccac tcatcaaaag
    11101 ccatatcaag aaacggcgct ttggtcattg cacccgcatt attcaccagc acatcaatac
    11161 gcccaaatcg ctggatgagt gtctccagcg cctgcgcacc ttctggcaga ttgccgagat
    11221 ccagttgcac gatctccgca cgtacgccgt gggtaaccac ctcacgcgcg gtatcttttg
    11281 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgatgc gccagtaata
    11341 acgcgcactc tttgccgatc cccgaatcgg aagcggtaat tatcgcgacc tatgccatcg
    11401 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg
    11461 taacccccgc ccagcgcacc ggtgagtcga atggaggcat ccagccactg cccttgcaat
    11521 aacaggccat tggcccgctc acgcagcgcc gggattctgg cttcgctgac gcgggaacca
    11581 gcaatgatgc ccgcgttaaa gcgggcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    11641 tcaatttgcg cctgatgctg gtttttctcc gccagtgtct gaacctgact ggctgcccgc
    11701 gccacgtcat tcactgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    11761 tcggcttttg cgatatcgag attggcgtta agacgaccac tatcgaaaat aggtagcgtc
    11821 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    11881 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    11941 tcaatagtgc ttaacgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    12001 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    12061 ggtttaagct tcaatgactt agtctggtga tttgtgagtg catttaaccg tgcctcaata
    12121 attttcattt tccccacgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    12181 gtttccatac cgtcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    12241 ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    12301 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    12361 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    12421 cagatatcga gatgccagcc cgccgttaag ccaaaagttc cgttggtgta ccacggaccg
    12481 gtcgtacctg cggccggatc gttcagagca aacggcccca ttagcccttc tgccgacatt
    12541 ttttgccgct ccatatccgc cgaaaagtcg atctgcggac catcctgagt ggcaactgcc
    12601 ttcgcctggg cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    12661 tgtagtgcat tgttaattaa tgaagtgagc tgattatcgt gatactccag ccaccattgg
    12721 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    12781 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    12841 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    12901 ggtaagaaac ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    12961 caatacatca gtagcaataa aacgacatac ccagaaaaat atagactaag taaatgatat
    13021 cttccgattt atcttaatcg tttatggata acggcaa
//
LOCUS       DAJTRQ010000110        12952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000110 DAJTRQ010000000
VERSION     DAJTRQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(56..2005)
                     /locus_tag="OO332_003166"
     CDS             complement(56..2005)
                     /locus_tag="OO332_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5943547.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2090..2426)
                     /locus_tag="OO332_003167"
                     /pseudo
     CDS             complement(2090..2426)
                     /locus_tag="OO332_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2426..2782)
                     /locus_tag="OO332_003168"
     CDS             complement(2426..2782)
                     /locus_tag="OO332_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5943548.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2782..4278)
                     /locus_tag="OO332_003169"
     CDS             complement(2782..4278)
                     /locus_tag="OO332_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5943549.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4275..4436)
                     /locus_tag="OO332_003170"
     CDS             complement(4275..4436)
                     /locus_tag="OO332_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5943550.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4446..5009)
                     /locus_tag="OO332_003171"
     CDS             complement(4446..5009)
                     /locus_tag="OO332_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943551.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(5006..5530)
                     /locus_tag="OO332_003172"
     CDS             complement(5006..5530)
                     /locus_tag="OO332_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943552.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5496..5912)
                     /locus_tag="OO332_003173"
     CDS             complement(5496..5912)
                     /locus_tag="OO332_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5943553.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5909..6232)
                     /locus_tag="OO332_003174"
     CDS             complement(5909..6232)
                     /locus_tag="OO332_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5943554.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6277..7380)
                     /locus_tag="OO332_003175"
                     /pseudo
     CDS             complement(6277..7380)
                     /locus_tag="OO332_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(7381..7996)
                     /locus_tag="OO332_003176"
                     /pseudo
     CDS             complement(7381..7996)
                     /locus_tag="OO332_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            complement(7974..9215)
                     /locus_tag="OO332_003177"
     CDS             complement(7974..9215)
                     /locus_tag="OO332_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5943555.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9215..9397)
                     /locus_tag="OO332_003178"
     CDS             complement(9215..9397)
                     /locus_tag="OO332_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943556.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9409..11166)
                     /locus_tag="OO332_003179"
     CDS             complement(9409..11166)
                     /locus_tag="OO332_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5943557.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11166..11648)
                     /locus_tag="OO332_003180"
     CDS             complement(11166..11648)
                     /locus_tag="OO332_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5943558.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11796..12146)
                     /locus_tag="OO332_003181"
     CDS             complement(11796..12146)
                     /locus_tag="OO332_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5943559.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12189..12653)
                     /locus_tag="OO332_003182"
     CDS             complement(12189..12653)
                     /locus_tag="OO332_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5943560.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12650..12952)
                     /gene="rrrD"
                     /locus_tag="OO332_003183"
                     /pseudo
     CDS             complement(12650..12952)
                     /gene="rrrD"
                     /locus_tag="OO332_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
BASE COUNT         2912 a         3823 c         3013 g         3204 t
ORIGIN      
        1 attgttagca tgttacgcga taattttgcc tcatctttca ccacaaaaaa agaactcacc
       61 ttttaaatgg agaataccca acatcatggg tgattttcat aaagggatcg cctgttttcg
      121 gcaaatcgat aacgcgcatt ccaggcggcg cgttgtcaaa cgtcacctta agttcgctgc
      181 gggttgccgg tgaagatgca aggcttaaca gctgattcct ttccagtgat acattcgggg
      241 tgtagcgttt atcgggagat aaatacccat caccacgtgg catctgccat ccggtcatgt
      301 catagacaaa atcatggaac tgtttgcccc actcgtcgaa cttatcattc agaccaaatc
      361 cactgttcag tgcatcagcc acaaaatttt taacaagcca gggatgttct ttttcaaact
      421 cctgcgccat catgcccagt tcaagcaatc caccaatcag actgatcttt ccgaggccct
      481 ttaatttcag ccctgttttt cctgccgccg ttgtccattg attctgggcg actgttgccg
      541 cacgaatact tgccaccgcc ttcacaccaa tatacagggc agaaaccgtt gctattgttt
      601 ttactgcctg ttcccatccc cccattgctc tgacaacctg atcaacctcc tgccagactt
      661 ttttcacaac cggaccgacc gtttcccagt tatcaattat gagatatgca ccaccgacga
      721 gaagcgcaat taatcccttt gccggcgtca tattcatcac gctacccatg atttttgtta
      781 tgcgtgtcaa tgtgccaatc gccacgccca tcgtcagtaa tgccgcacca gttttcgcga
      841 tggtttttac gacctcagga ttttctctga caaacgtgcg cacttcttcc agaaaaggct
      901 tcatctcttt tatgccttca ttgagtgaag gcaggaaggt ttcccccagc gtggaagaaa
      961 tcgcattgat ctggttctgc agcagcaaca actgattttc tgtcgtcgcg gcacgggcgg
     1021 catattcttt ctgcattgag ccgccatact gctgcgcatc cgccacacga ttaaagttgg
     1081 ttcgcaacaa atccagattc gtgagcagcg gcgcaatggc ccccagggat tccttcccga
     1141 acagcgcttt cagcacagca gcctgttttt ctttcggcac ttttgccaga gaatccagta
     1201 cgtgcagcat ggccccgcgg gcgtctttct gcatatccgc cgccagtttt ttcgggctaa
     1261 tgcgcaacga gcggagcact tttttctgcg atttggtggc agaatccctc gctgtcagcg
     1321 acaacataaa attctttatc cccgtcgcag caatttctga ctccaccccc attccggcaa
     1381 tggttgcccc cattgcggca atctctccgg aggccacacc cgcaacactg cctaaagggc
     1441 caatacgggt cacaacatca gaaatctttt tcgcacttgc aggaccggta ttgccaagat
     1501 agttaatctt atccgcaagc cctgccacct ctccctgtgt cagtttaaag gcagtgcgcc
     1561 actgcgccat catctgaccg gattcttctg ccgtggcgtc aaaggccacg cccatcttca
     1621 cggcatcgtc agtaaactgc atcagctcgt cacgggcgat accagcctga ccacccgccg
     1681 ccacaatttc ggcgatacct tccgccgaca tgggcagttc tgttgacagg tcgcgcacct
     1741 gctccgtcat tgccttaaac gcttccggcg tatccagacc atccaccact ttccggacat
     1801 ccgccatttt tgattcaagg gcaatggcag atttgaccgg gagcgccagc gcccccagta
     1861 ctgcggttcc ggcacccgct gcgcccagtg acaggctggc aaactccttt ttaaaacctt
     1921 tcagctggcg ctgcattccc ttcagcggtg ccgatacctt atccacggca gtgatgatcg
     1981 ccttcagctg aaaactgtca gccatgcttc atctcctcgt taatgcggac ggcctcggct
     2041 tccagttctg taaactgcga aacagccacc cgtcgcagct ccagtggatt cagtttccag
     2101 aaccaggcaa cattgtagag tcgtttcccg gaggtgcttc ccgtctccga ctgggtaaaa
     2161 aaacgcagga tctgcatgct ggtcttaaaa atatccagtt ttgccatctg cgccgccgat
     2221 gaacgcggga tccccgccag caacgggata tatttcagtg ccacctggct gtccagttta
     2281 atactgcctt caccggaaat aatgaaagga aaacccagcg cctcgatttc gtcatacgtg
     2341 ggctcgcgca actccagcac atgcagcatt tcgttatgcg ctgtcaccgg tttttttaaa
     2401 actatttctg taacgttcat tgccattact gataccctcc ctcttcgccg tggaattcaa
     2461 gatctgccgt gccttcttcg gcattatgat tagcctctcc gtgcagccat gccgccgaaa
     2521 gcacatacac catgccgttt gccagctcag cggtaatcgt catctggtct gaggtggtaa
     2581 ttttgtcgac cggaaagttt ttgggcactt taaacgtgcc cttaacgtaa ggtgaccgcc
     2641 aggtctcctt gtaatccaca tcccctgcca tgccgacgat gtcatcccgg acattggtgt
     2701 tcatcggcac ttcaatgcca cctgtcagcg ataactgctg accatccact ttaaaaaaac
     2761 aggttccggc gatctttgcc attatgctga ctcctctgaa tactgaagac ggaactggtt
     2821 aaccaccgca aagacacgta actgattaac ataatccggc gggaacagcg tattcagtcg
     2881 gttcgggtta tctgcatcac gctcaactat cagatactgt ttaaacagat cgtaattttc
     2941 cacaataccg gcacgctcca tctgacgata tgtcgccaga agttcccctt tgataacggc
     3001 aggagtgaca atcgcctgcc ccggaccaaa acgggtacca tcatttgcca gcttgtgacg
     3061 tccgtacttg cttgtgatga ccgatttcag tttgcgaaga acatgtgcac tggtatgcag
     3121 agtttcactg tccagatagc tgttgtctgc cacgccatac gcattctttt tgtaggtggt
     3181 tacggaacgc tggatctgca acgtgccgcc ttccacataa gccgtcgcca cgccgtgaga
     3241 taaaagggtc tgctgctctg tcatgatgaa tcgcttacct ttcggtgccg gaagcatccc
     3301 caccagctcc cccgtctgtg tcggacgggc aggatcattc cggataaata ccgcttcacg
     3361 ggcaaggcga ctggcaacca gttcatcgac aggcgactgg gtttcttttt cgtaaccggc
     3421 aagcgtgata tgttgctgat tatgcatatc tccggcacca accagctctg acagcgttcc
     3481 cagttttgcg gtatagacat gcccgtataa ctggcgcgca taactccagc gaccgctgct
     3541 gtcattcatt tcggtcatca tcatattgat ggaggcggca tcgttgaacg gcagaccgat
     3601 aaagtcgaat gcctcatcgc ccatagcagc aacagcggcg gtaagatcag gcgctccgct
     3661 accggcagca ccgacttccg tcaccacccg aagccctgct ggcagaatct caccaccacc
     3721 agaaccataa taattcagac agaccggtaa ctcattacca tacagcccct tatgacgggc
     3781 agtcagcgtc accacgcctg catcagatga cgccgtaaac ggcagggtga taaccccgtt
     3841 taccgcttcc ttaatcgcgg tggcaaccgc agtggcatca tcgccattca ccacaggcac
     3901 ctgtacacgg gagcgcccga catacagact cagggtgccg ctttcctctg cttctccggc
     3961 aaccgtcacc ctgaccgtcg ccgccgcccc tctggcttcc ggtactgcaa taacatacag
     4021 ttcaccgaaa ggatctgtct gacggtagac ttccaccata cgcgccagct ggctccccgc
     4081 cccacaaatc tggcgggcat aatctgccga tggcatcagc accaggctgt taacctcaat
     4141 ggctgcatcg ttgctggcat gcccgatcag taatgcaggc gcgctggtca ccgctgtatt
     4201 tgccgcagaa ttgttcatct cggcgtagaa caacggcacc agcgtattcg acggaacagt
     4261 attaaaactt atcgtcatga ttttttagcc ttatcctgtt taacacgtac cacatcaccc
     4321 gccgctatac ggcggaacca gtagctgctt tcttccacat ttcgcccttc ggaaggcaaa
     4381 aggtctccac gggcagggtc aggaaccgac cgccctttca ggggtttcac aaacatgagg
     4441 gactcttaat tctgagggaa acgcatttcc agatggtgct caatgtcacc atccgggcca
     4501 gtaccaggat cgatgtaatc cacatcaata ctcagcaggg ataaatccgg cagggcattc
     4561 acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt aaaatcaaac
     4621 tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata aacaatctcg
     4681 cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct ccggacatca
     4741 tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct gagcaccaca
     4801 atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt ttgttccccc
     4861 ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc ggcatcaggg
     4921 atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca tcgctcacgc
     4981 agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga cgtaacgaac
     5041 gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca ataacgtcag
     5101 ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc ttctgccgct
     5161 tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga aacgccggat
     5221 aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg tcaggggaaa
     5281 ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa ccaatggagc
     5341 gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt ccgcgtttaa
     5401 tcaccagacg tctggcatca cgcatgtaaa cgcgcccgat ttgcacaaac gcccgacgca
     5461 gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt aaaaatgctg
     5521 tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca ttccagtaat
     5581 aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc ttggccgtaa
     5641 taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa ataatgcgta
     5701 acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg agccaccttt
     5761 gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc ggtgggttca
     5821 tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg taaaaggtaa
     5881 gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa gggccagcaa
     5941 gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt gagcgatttt
     6001 cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc agatgcatcc
     6061 cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg ttcaggtatg
     6121 tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg tcactgtaca
     6181 aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc atattttcct
     6241 cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt tctggcgggt
     6301 tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac cgccggttcg
     6361 cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca ggatacgggt
     6421 accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa tggcttttgc
     6481 atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg ccagagaaga
     6541 aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat ccttcagcag
     6601 acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc ggtgcgcctt
     6661 acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag ccgcgccgga
     6721 agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg tggactcata
     6781 agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa tttcttcctg
     6841 ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa agaaagcatc
     6901 atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa agggctcaat
     6961 cagtcccagt tttgaggtgg cggtttccgg acgcgcttcc gtttccccca cccatccgga
     7021 cgtcgtgccg cccagattca ccagtttttt ataatccgag ccactcagag tgattacggt
     7081 ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc gatccagttc
     7141 ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct ttcgctcaag
     7201 ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt ctttatgttc
     7261 cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca gctcagcctc
     7321 aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt ccacttctcc
     7381 gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat agcccccggc
     7441 catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc tttcgatttt
     7501 tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga tccgcgcctc
     7561 gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt tcagcagaaa
     7621 ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag aaaggccggt
     7681 taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat catcaataag
     7741 caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt aaacaccgat
     7801 gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt cactccacgc
     7861 ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt cctttacacc
     7921 aaagacggag ccatgccctt caaactcacc ggagtcactg acagattggt acatcaagac
     7981 gttgtttcgt ctgcattggc gttatccttc tgcttaccgg ctttactgcc atcggagggt
     8041 ttcgtggtca tgttcatcgg tgtgagatag acatccccac cgggacgcgg attcatatct
     8101 tccaggtcgc ggcagtcatt gggagagtaa atcccccagt taatcccggt ggcgtaggct
     8161 tcaaaacggg atttcatatc cccgcgcagt aacgccccgg cgttaaattt ggcgtaaaac
     8221 ttcccctgtt tgctctccct gaccagccct gtattgatcc gttgttcaat acgggtcaga
     8281 tacggcacaa gggaatagtt gataaatccg agccccagct cttcaatatt gttgaaagtg
     8341 gcgcgatcgg tgttctgcac catgtgcagc ggcacgcgga aaagacgaca gatttcttcc
     8401 aactgaaact tgcgggtttc caggaactgg ctgtcctcgg cattcagtgc caccggcttc
     8461 cagtccagcc ccatttccag aatcatcgga cggtgcgcat tacccagccc aagatgacgc
     8521 tcctcaaaat ccctcttcat gcgctcataa gcatccggcg tgagcttttg ttccgtgcgc
     8581 aacacaccgg atgtcaccgc accattacca aacaacctgg caccgtgctc ctcggttgcc
     8641 gctgccagtg aaatggcctc gcgcgcatat gcaatgggat tcagcccgac cagtccgtcc
     8701 agcgtcaggg tgcgcacatg ccagatttca tcctgggtca acacatccac ggaaccatcc
     8761 ggaaacgtca cctgataaac cggctgccac tggctgttca gcttcggttc cacacagccc
     8821 ggatctatcg gaagaagctc cacaacttcc cccagcgcct tcaccttgta agcgtaaaaa
     8881 ttcccccgca gacacaggca gacgataacc agctcccaga attcctgcgg cgtcatgtag
     8941 ccattgggtt ttgctgaaat cagcttatgc agccgttcat ccaccgcccg tgttttaagg
     9001 gtgccgctga ttttgtagag actgcagggc agcataccaa cagactcagc cagcaccctg
     9061 acgcaggaat agaccgccgt cagccgcatg gcccgctggc tgctgatccg ctttccggta
     9121 taggtgtcgt atgacaaccc cagctcttcc gcaagcatcc cgggcgttgt gacgggggtg
     9181 ttatttttgc gttgaaaaag cccctggaaa aacattactc acctccggag gcgacccggt
     9241 gaccgcgatc gagataacgc gcaaccagcc acgaccagca cagacacagc gccccggcaa
     9301 caacaaaacc tgccggggga taaatcagcc atgtgccata cgacagcaaa agcgccccca
     9361 gcacgcccac cagtggcgtg agaattatca gaaacataat gacctcggtt aaagcgagcg
     9421 aataccaacg ctgaccagat gctcagacag atccggctcc ggttcaccgc cattgaccag
     9481 catccggctc attgctgtaa acatcgcaac agggccgtcg attttggctt ccggcgtgga
     9541 tttgttcggg aagatattgt cgtttttgtc cggtttcacc gtaacgttag acatcatcca
     9601 gttcatgacc gggtgattac tgtgatggaa acgcccggca tagaccagtg attccgtttc
     9661 cttcatggcc tctgacagat tgcggaccgt ctgcggaact tccaccagcg gtataccttc
     9721 ttcagccagc gccagactga actgcattgc gctccacggg tcaaatccca gttccctcag
     9781 gttttcaccg ccaatccatt ccagtaagtc actttttatc tgagcatgat cgataacatc
     9841 accatccgtc agaatcagct tatccatctc cgcccacttc cggtaaagtt ctgcctgctg
     9901 ccgcgagcat cgttccagcc gtccttccgg aagccagaat ttaaaatcgg catgaacatg
     9961 cccgttatcc gttcgccaga gttttgccgc cgcacagata tcaatcttat gagcaaggtc
    10021 aacgccgacc cacatgggat acgttttcag ctcatgtcgt ggggcaatgt attcgcactt
    10081 ctcccactta atcatgtcca tccaggcaga ctctgctgtt acccacacat tcatgtgttt
    10141 ggtaaaaaaa ttcacccgcg cagagacctg ttctttcgct tttttcgcca ggcgacgcag
    10201 atcatcccag cgtttacaga tgcccaggcc gggattcgct ttctgccaga ccgtttcatc
    10261 aaacggatca tctccctcat cgagggtgta aataatcgca aagtaggagt cgtcttttac
    10321 cgcgccctcc acgtcgctgt tatagccacg caataccttg atggcgtaat cacgctgctc
    10381 gtaacaaatt ccttccttgt taaaccctgc cgtggtgata ccaaataaaa gggactgcag
    10441 acgggcaccg gttgccgttt ccagaacgtc ccacacgtca cgggttttat gtgcatgcag
    10501 ctcatcaata atggcgcagt ggatgttcag accatccagg ttgtttgcat ctgaagaaag
    10561 cggttcaaat tttgatgcgc tctgctcctg ataaatcgcc agcttgttga aatcaaacaa
    10621 ccgcccgagt gtcgaccggg cttttctgac catatttttg gcgtcttcaa acacgattct
    10681 ggcctggtca cgcgtggttg cggctgaata cacctcagcc ccgccttcac catctgcccc
    10741 cgtcatatac agaccgatac ctgctgacag ggttgatttt gcgtttttac gggcgacttc
    10801 gttgtacgct gtccggaaac ggcgcaccat caccgggcgt ccgctgccat cgctgcgcat
    10861 gacaacttcc ccggtctctt cattcaccag cggaatgaca aaaccaaaaa tattaatgag
    10921 gataaacaca tgccagtcca tcaactcaat gggctgacct gccagcgccc cttttacatg
    10981 gggcacaaat ttgtagaaat tcaggatgtg ctgcgcacgg ggttcactga aataaatccc
    11041 ccgctcttcg ccgtacttca gatcatcaag aaaacgctgg caggccagac ggacaaattc
    11101 accagcgaca atttctcctg caacaacacg ttcggcgtag cggatcccgt cagccacttt
    11161 tgccatcagt ctctcgcttt taaaagttct gccagcggat caacatcatc cggtccggcg
    11221 atatttactt tcgcccggct tgccggtgac ataccaaatt ctgcaagcat cgcccggatc
    11281 cgcttccagg catccgcttt catcgcagca gccgggtgtg ccttaatcag cacatcaccg
    11341 ttctgcgttt ccgtgcggta ggtataaccc tcaacatcga gggtttcgca gtgatgccgg
    11401 tattcggtgt aggcttccac cagcaactcg agtgcacgcg catcaagctg agaaatgatc
    11461 ccttccgcat tcagctcttc cgccattcgc ctgaaccagt actttccctg cgcccctaaa
    11521 tgctgcggaa ttttagggag acctttttcg tcctttttag cggttttttt ggcgtcttta
    11581 acgggacgct ttgagggatt gcctcgtatc aaatgcaggc gtggcggggt tttcgggggt
    11641 cctgacataa tcgattttac ctatcaatcg cttgatcgca ttcccaaaaa aaagttttcg
    11701 aacctgcggc gatgcgagga agggttggcg ggcggtcccg gacagccagg gctgcaggga
    11761 tttgacccgc ccctccctac aagtgagaat aattatcacc tgattcgttc gcgcgctgtt
    11821 ttcgctttat ggcagggcca gcacagactc tgcaggttgc tgtctgcgtc tgttccgcca
    11881 tgcgctttcg ggatgatgtg gtcgacggtt ttcgcctcgc tcaccacacc gacacgcaga
    11941 cacaactgac acagaccttt atcgcgcttc agaatacggg cacgaatcac cgtccatttt
    12001 gagccatagc cacgctggtg gcggctcagt ccgcgctggt gctgcaccca gccttcaccg
    12061 cgatgcttat cgcagtaacc cgaactgtct gtggttgtac ctgcacatcc acgcttacgg
    12121 caggcacgtg ggattagtga tggcataaat acctcatacc ctgcgaaatg tttaccacga
    12181 taaaaaggct acttaatgca ctgagtgcgg atatactcct gtgccccttc cagttgcatc
    12241 tgcatcgtca tcagccgctc tctgagggtg aaataatccc gttcagcggt gtctgccagt
    12301 cgggggctgg ttgcattatc cacgccggag gcggtggtgg cttcacgcac tgactgacag
    12361 actgctttga tgtgcaaccg acgacgacca gcggcaacat cagcgcgcag agtttcattt
    12421 tcagctttcg catcagctaa ctccttcgtg tatttagcat cgagtgcatt aacatcacgc
    12481 tgtctctgct gcatgtcagt aatggttgcg ttcgccagct tcagttctct tgcgtttttg
    12541 tcgcgctgct ctttgtaggt aatagcgtta tcacggtaat gattaacagc ccatgacagg
    12601 cagacgatga tgcagataac cagagcggag ataatcgcgg tgactctgct cattgctgcc
    12661 cccacaaaca gacttcacgc tcaatctcac gacgggtcat cagccctttc cattgcttac
    12721 cgccagcata tgtccagcga cgtagctggt cacatgcgcc tttgatatcg ccctagttta
    12781 ttttgcgaag aagcatcgat gttctgaaat tgccagcgcc cacgttgtaa acgaacgagt
    12841 aaagagcgcc gcgcgttgtt tccggtatat cgactttgat gtacgggtta atttgtctgg
    12901 cgacagtggc aaggtcttta ttcaggaggg ctttgcattc tgcttcggta ta
//
LOCUS       DAJTRQ010000111        12947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000111 DAJTRQ010000000
VERSION     DAJTRQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..615
                     /gene="mxiE"
                     /locus_tag="OO332_003184"
     CDS             <1..615
                     /gene="mxiE"
                     /locus_tag="OO332_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5943561.1"
                     /translation="VRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALVEHSHAE
                     KCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRT
                     IEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTI
                     TSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            602..2302
                     /gene="mxiD"
                     /locus_tag="OO332_003185"
     CDS             602..2302
                     /gene="mxiD"
                     /locus_tag="OO332_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5943562.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            2321..3388
                     /gene="mxiC"
                     /locus_tag="OO332_003186"
     CDS             2321..3388
                     /gene="mxiC"
                     /locus_tag="OO332_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5943563.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            3401..5461
                     /gene="mxiA"
                     /locus_tag="OO332_003187"
                     /gene_synonym="virH"
     CDS             3401..5461
                     /gene="mxiA"
                     /locus_tag="OO332_003187"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5943564.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            5474..5875
                     /gene="spa15"
                     /locus_tag="OO332_003188"
                     /gene_synonym="spaK"
     CDS             5474..5875
                     /gene="spa15"
                     /locus_tag="OO332_003188"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5943565.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            5879..7171
                     /gene="spaL"
                     /locus_tag="OO332_003189"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5879..7171
                     /gene="spaL"
                     /locus_tag="OO332_003189"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5943566.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            7264..7620
                     /gene="spa13"
                     /locus_tag="OO332_003190"
                     /gene_synonym="spaM"
     CDS             7264..7620
                     /gene="spa13"
                     /locus_tag="OO332_003190"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5943567.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            7589..8467
                     /gene="spa32"
                     /locus_tag="OO332_003191"
     CDS             7589..8467
                     /gene="spa32"
                     /locus_tag="OO332_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5943568.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            8461..9342
                     /gene="spa33"
                     /locus_tag="OO332_003192"
                     /gene_synonym="spaO"
     CDS             8461..9342
                     /gene="spa33"
                     /locus_tag="OO332_003192"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5943569.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            9347..9997
                     /gene="spa24"
                     /locus_tag="OO332_003193"
                     /gene_synonym="spaP"
     CDS             9347..9997
                     /gene="spa24"
                     /locus_tag="OO332_003193"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5943570.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            10012..10272
                     /gene="spaQ"
                     /locus_tag="OO332_003194"
     CDS             10012..10272
                     /gene="spaQ"
                     /locus_tag="OO332_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5943571.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            10272..11042
                     /gene="spa29"
                     /locus_tag="OO332_003195"
                     /gene_synonym="spaR"
     CDS             10272..11042
                     /gene="spa29"
                     /locus_tag="OO332_003195"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5943572.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            11049..12077
                     /gene="spa40"
                     /locus_tag="OO332_003196"
                     /gene_synonym="spaS"
     CDS             11049..12077
                     /gene="spa40"
                     /locus_tag="OO332_003196"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5943573.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            12090..12332
                     /locus_tag="OO332_003197"
     CDS             12090..12332
                     /locus_tag="OO332_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943574.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         4156 a         1839 c         2526 g         4426 t
ORIGIN      
        1 gtccgaaatc aaaatataaa attcagtgat aatgtgaact atcattatcg ctttaacatt
       61 aatagttgtg caaaatttct tgctttttgg gactatttca gtggtgctct agtagagcat
      121 agccatgctg aaaaatgtat tcatttctat catgagaatg atcttcggga tagttgtaat
      181 accgaatcga tgcttgataa gttaatgtta agattcatct tttcatctga ccagaatgta
      241 agtaatgcac tggctatgat acgaatgact gagtcatatc atttggttct ttatcttctg
      301 agaactattg agaaagaaaa ggaggtaaga ataaagagtc tgacagagca ttatggtgtg
      361 tcggaggctt attttagatc tttatgtaga aaagctttag gggcaaaagt caaagaacaa
      421 ctgaacactt ggcggttagt aaatggttta ttagatgtat ttttgcacaa tcaaacaatc
      481 acttctgctg ccatgaataa tgggtatgca tcaacatccc atttttcaaa tgagataaaa
      541 acacgactag gcttttctgc aagagaatta tcgaatatta cttttttagt gaaaaaaata
      601 aatgaaaaaa tttaatatta aatctttgac tctcttgatt gtattgttac cattgatagt
      661 taatgctaat aatattgact ctcgtttatt agaacaaaat gacatagcga aatatgttgc
      721 tcaaagtgat acggtaggat ctttttttga acgtttttct gcgttattaa attatcctat
      781 tgttgttagc aaacaagctg caaaaaagag aatatctggg gagtttgatt tatctaatcc
      841 agaagaaatg cttgaaaaac tgacattact tgtgggactc atatggtata aagatggaaa
      901 tgcattatac atatatgact ctggagaatt aataagcaaa gttattttgc tggagaatat
      961 atcacttaat tatcttattc agtatctgaa ggatgctaac ctttatgatc acagatatcc
     1021 tataagagga aacattagtg ataaaacatt ctatatatct ggtcctccgg cattggttga
     1081 actggtagct aatacagcaa ctttgcttga taagcaggtt tcttctattg ggacagataa
     1141 agttaatttt ggggttataa aattaaaaaa tacatttgtt agtgacagaa cttataatat
     1201 gagaggggag gatattgtca tcccaggggt ggctactgtt gtagaacgtt tattaaataa
     1261 tggtaaggct ctgagtaaca gacaagccca aaatgatccg atgccaccct ttaatattac
     1321 acagaaagta tctgaggata gtaatgactt ttcatttagt tcagttacca atagttctat
     1381 attagaggat gtatcactta ttgcatatcc agaaaccaat tctatattag tcaaaggtaa
     1441 tgatcagcaa atacagatta ttcgcgatat tatcactcag ttagatgtag ccaaacgaca
     1501 tatagaatta tcattatgga taatcgatat tgataaatct gaattaaata accttggtgt
     1561 taattggcaa ggaacggctt catttggtga ttcgtttggt gcgtcattta atatgagctc
     1621 atcagcatcg ataagcactt tggacggcaa taagtttatt gcttcagtta tggcattaaa
     1681 tcagaaaaag aaagcaaatg tagtttcaag gccggtgatt ttgacacaag agaatattcc
     1741 ggctatattt gataataaca ggacttttta tgtgtcatta gttggtgagc gcaattcttc
     1801 gttggagcat gttacgtatg gaacattgat aaatgttata cctcgctttt catctcgggg
     1861 acaaattgag atgtctttaa cgatagagga tggaacagga aatagtcagt caaattacaa
     1921 ttataataat gaaaatacat ctgtccttcc agaagttgga aggaccaaaa tcagtacaat
     1981 agcgagagtt ccacaaggca aaagtttgtt gataggtggt tatacgcatg aaacgaactc
     2041 taacgaaata gtatctattc cttttctctc ctctattcct gtaatcggta atgtgttcaa
     2101 gtataaaaca agtaatatca gtaatattgt aagggttttt ctgattcaac cccgtgagat
     2161 aaaggagtca agttattata atactgcaga atataaaagc ctcatatctg aaagagaaat
     2221 acaaaagact acacaaataa tcccatctga aactactttg ctggaagacg aaaaatcatt
     2281 ggtttcatac ttaaattact aactataaag taggtgatgt atgcttgatg ttaaaaatac
     2341 aggagttttt agctctgcat tcattgataa gttgaatgca atgacaaatt cagatgatgg
     2401 agatgagact gctgatgcag agcttgattc tggcttggct aatagcaagt atattgactc
     2461 atctgatgag atggcttccg ctctttcgtc atttataaac agaagagacc ttgagaaact
     2521 gaaaggaaca aatagtgata gtcaggaacg tattttagat ggggaagaag atgaaattaa
     2581 tcacaagatt tttgatttaa agagaacgtt aaaagataac cttcctctag atcgggattt
     2641 tatagacaga ctaaagagat attttaaaga tccaagtgat caagtcttag cattaaggga
     2701 acttttgaat gaaaaagatc ttactgctga acaagtcgaa ttattaacta aaattattaa
     2761 tgagataata tcaggtagtg aaaaaagtgt taatgctgga ataaattcag ctatacaggc
     2821 taaattattt ggcaacaaaa tgaaacttga accacagctt ttgcgtgcat gttatcgtgg
     2881 ttttatcatg gggaacatat caacaacaga tcagtatata gaatggcttg gtaattttgg
     2941 ttttaatcac agacatacaa ttgtgaattt tgtagagcag tcactgattg tagacatgga
     3001 ttctgagaaa ccgagctgta atgcttatga gtttggtttt gtgttatcta aattaattgc
     3061 aattaagatg attagaactt cagacgtaat ttttatgaag aaactggaat cctcaagctt
     3121 gctaaaagat ggcagtttaa gtgcagagca gctattgcta actttattat atatttttca
     3181 atatccaagt gaaagtgagc aaattcttac ttctgttata gaagtatcac aagccagtca
     3241 tgaggattct gtagtgtatc aaacatatct atcttctgtt aatgaaagtc ctcatgatat
     3301 atttaaaagt gaaagtgaaa gagaaattgc gatcaatatt ctacgagagc ttgtcacaag
     3361 tgcatacaag aaagagcttt ctagataaca ggagataaaa gtgatccagt cttttcttaa
     3421 gcaagtaagt actaagcctg aattaattat attagtgctt atggttatga tcattgctat
     3481 gttgatcata ccattaccta cataccttgt tgatttttta atagggctta atattgtact
     3541 tgctattctc gtttttatgg gatcatttta tattgaaaga atattgagtt tttctacatt
     3601 cccttcggtt ttgttgataa cgacattatt tcgtctcgct ttatcaataa gtactagtcg
     3661 actaatttta gttgatgcgg atgcgggtaa aattattact acatttggcc agtttgtcat
     3721 tggtgatagt ctggctgtag gttttgttat tttttcaata gtaactgttg tgcagttcat
     3781 tgttataaca aaaggatctg aaagggttgc ggaggtagct gctcgctttt ctcttgacgg
     3841 tatgccgggg aaacaaatga gcattgatgc ggatttaaag gctggaatta ttgatgccgc
     3901 aggagctaaa gaaagacgca gtattctgga acgtgagagt caattgtatg gttcatttga
     3961 tggagcaatg aagttcatta aaggtgatgc aattgctggc atcattatta tttttgtcaa
     4021 tttaatagga ggtatttctg ttggtatgag tcaacatgga atgtccctat ctggtgcatt
     4081 atctacttat accatactaa ctattggtga cggattggtc tctcagattc ctgctctgtt
     4141 aatttctata agtgcaggat ttatcgttac aagagttaat ggtgatagcg ataatatggg
     4201 acgtaacata atgtcccaga tatttgggaa tccttttgtt ctcattgtta catcagctct
     4261 tgccttggcg atagggatgt tgccaggttt tccgtttttt gtttttttcc tgatagcagt
     4321 tactttgacg gctttattct attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga
     4381 gtctgattct agtggctata ctggtacatt tgatattgat aatagccatg actcatcttt
     4441 ggcaatgata gaaaatctgg atgctattag ttcagaaacc gttcctttaa ttttattatt
     4501 tgccgaaaat aagataaatg caaatgatat ggaaggtctt attgaaagga taagaagtca
     4561 gttctttatt gattatggtg tcaggcttcc aactatttta tatagaacaa gcaatgagct
     4621 taaggtcgat gatattgttt tgttaataaa tgaggtgcgt gccgatagtt ttaatatata
     4681 ttttgacaaa gtgtgtatta cagatgaaaa tggagatata gatgctctag gtattcctgt
     4741 tgtttcaact tcatataatg aacgtgttat ttcttgggta gacgtttcat atacagaaaa
     4801 tctaactaat attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc
     4861 acaagcttta ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt
     4921 agatcagttt gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat
     4981 acagagaatt tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt
     5041 aaaacttatt atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt
     5101 agttgaacat gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg
     5161 tgagattaaa gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag
     5221 gcaaacctct ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga
     5281 aactttagca catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt
     5341 atcagtagat atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact
     5401 cgttatatcg tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta
     5461 gtgaggttta aatatgagta acattaattt agttcaatta gttagagata gtcttttcac
     5521 gattggttgt cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc
     5581 tttggatagt atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc
     5641 taattttgat gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt
     5701 aatgttaatg aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga
     5761 gtacttacag ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc
     5821 agagattctg catgagtttt atcagaggat ggaaatctta aatgaggtct tataatcaat
     5881 gagctataca aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt
     5941 ggaaacaagt cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga
     6001 aagtaatgaa attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt
     6061 aagcttgatt ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca
     6121 gtttcttcat acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga
     6181 ggttactgat aagtttgctg ttacggataa cagtgaaatt ctttatcgac ctgtagataa
     6241 tgctcctccg ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa
     6301 ggttattgat tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc
     6361 tggttgtggc aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata
     6421 tgttattggg ttaattggtg agcgaggtcg agaggttaca gaaacggttg attatttgaa
     6481 aaactctgag aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt
     6541 tgatcgttgt aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg
     6601 acataaagta gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt
     6661 ggccttagcc gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag
     6721 cttacccaga cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt
     6781 ttacactgtt cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc
     6841 cattttagat ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc
     6901 aattgattcc ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg
     6961 tattatggcc gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat
     7021 tgactttggt gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat
     7081 atctgttgtt gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac
     7141 aatggagctt attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac
     7201 ggtctgttaa attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga
     7261 gatgtggagg cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa
     7321 cctgtaggcg ctcagagcgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta
     7381 aaaaagcata ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa
     7441 attcaaaaag atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt
     7501 aaaatatgta aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt
     7561 gatgagttat cacaagagga tatgaagtat ggcattagac aatataaacc taaattttag
     7621 tagtgacaaa cagatagaaa aatgtgagaa actatcttca atagataata ttgatagtct
     7681 cgttttgaag aagaagagga aggttgaaat tcctgagtac tctttaattg cagctaacta
     7741 ttttactatt gataagcact ttgaacataa gcatgataaa ggagaaattt atagtggcat
     7801 taaaaatgcg ttcgaactta gaaacgaacg agcgacatat tctgatattc cagaatcaat
     7861 ggccattaaa gaaaatattt tgataccaga tcaagatatc aaagcaaggg aaaaaataaa
     7921 tatcggcgat atgaggggga tcttttcata taataagagt ggaaatgcag acaagaactt
     7981 cgaaagaagt catacttctt ctgtaaaccc tgataatctg ctagaatctg ataatagaaa
     8041 tggtcaaatt ggtttaaaaa atcatagctt gtctattgat aagaatattg ctgacatcat
     8101 ttctttacta aatggaagtg ttgctaaatc atttgagctc cctgtaatga ataaaaatac
     8161 tgcagacata accccatcca tgtcattgca agaaaaatca atagttgaaa atgataaaaa
     8221 tgtttttcaa aaaaatagtg aaatgactta ccactttaaa cagtgggggg ctggacattc
     8281 tgttagtatt tcagtggagt ctggttcttt tgttctaaaa ccgtcagatc aatttgtagg
     8341 aaataaactt gacttaattt tgaaacaaga tgctgagggt aattacagat ttgatagcag
     8401 tcaacataat aaggggaata aaaataatag tacaggatat aatgaacaga gtgaagaaga
     8461 atgctaagaa ttaaacattt tgacgctaac gaaaaactac agattttata tgcaaagcaa
     8521 ctctgcgagc gtttttcgat tcagacattc aaaaataaat ttacaggcag tgaaagttta
     8581 gtcactctta cttccgtgtg tggggattgg gtaattcgta ttgatacatt atcttttttg
     8641 aaaaaaaaat acgaggtatt ttcaggattt tctacacaag aatctttact gcatttatca
     8701 aaatgtgtct ttatagagtc gtcatctgta ttttcgattc cagaactgtc tgataagatt
     8761 actttccgga tcacgaatga aatccagtat gcaactactg ggagtcattt atgctgtttt
     8821 tcctcttctt taggtattat ttattttgac aagatgccgg tattacgtaa tcaagtttct
     8881 cttgacttat tgcatcatct tttagagttt tgcttaggtt catctaatgt aaggctggct
     8941 actttaaaaa gaattcgcac tggtgatata atcatagttc agaaacttta taatttatta
     9001 ttgtgtaatc aagttattat tggggattat attgtgaatg ataataatga ggcaaaaatt
     9061 aatctgtcag aaagtaatgg tgagtcagaa cacacagaag tttctttggc attattcaat
     9121 tatgatgata tcaatgtaaa agtggacttt attcttttag aaaaaaatat gacaatcaat
     9181 gaactaaaaa tgtatgtaga aaacgaatta ttcaagtttc ccgatgacat agttaaacat
     9241 gtaaatatta aagtaaatgg ttctttggtt gggcatgggg aacttgtttc tattgaggat
     9301 ggttatggta tcgagattag ttcttggatg gtaaaggagt aatctcatgc tgagtgacat
     9361 gtccctcata gctacacttt ctttttttac gctattacca tttttagttg ctgctggtac
     9421 ctgttatata aagttttcta ttgtttttgt aatggtacga aatgcacttg gtttgcagca
     9481 ggttccatca aatatgactc tcaatggtat tgcattgatt atggctttgt tcgtgatgaa
     9541 acccattatt gaagctggtt atgaaaatta tctaaatggc cctcaaaaat ttgatacaat
     9601 atctgatatt gtccgttttt ctgattctgg cttgatggaa tataagcaat acttgaaaaa
     9661 gcatacagat cttgagttag cacgtttttt ccaaaggtct gaagaagaaa atgctgattt
     9721 aaagagtgca gagaataatg attattcatt gttttccctc ttgcctgcat atgcgctaag
     9781 tgaaatcaaa gatgcgttca aaataggttt ttatttatat ttgccatttg ttgttgttga
     9841 tcttgttatt tcaagcattt tacttgcact tggtatgatg atgatgagtc caatcacaat
     9901 atcagtacca ataaaacttg tattatttgt tgcgcttgat gggtggggta tcctgtctaa
     9961 ggccttgatt gaacaatata tcaatattcc tgcttagatg atggaggtaa tgtgtctgat
    10021 atagtttata tgggtaataa ggctctttat ttaatcctta tcttttcctt atggccagta
    10081 ggtatagcta cggttattgg attatctatt ggtttgttac agacagtgac tcaactccaa
    10141 gagcagacac ttccttttgg tataaagctt ataggtgtct caatatcttt gctacttctt
    10201 tctggatggt atggtgaggt tttattgtct ttttgtcatg aaataatgtt tttaattaag
    10261 agtggggttt gatggacatt tcaagctggt tcgaaagtat tcatgtgttt ttaatactcc
    10321 tgaacggcgt tttttttaga ttggctccat tatttttctt tcttccattt ttaaataacg
    10381 gtataatttc tccatctatt agaatacctg tgatttttct tgttgcatca ggattaatta
    10441 cttctggtaa ggtagacata ggttcttctg tttttgaaca tgtttatttc cttatgttca
    10501 aggaaataat tgttggcctc cttctctctt tttgcttgtc tcttcccttt tggatatttc
    10561 atgctgttgg tagcattatt gacaaccagc gtggggcaac gcttagtagt tcaattgatc
    10621 ctgccaatgg tgttgatacg tctgagttgg caaaattttt caatcttttt tctgcagttg
    10681 tatttctata cagtggtggt atggtcttta ttttagaatc catacaattg tcttataata
    10741 tatgcccgtt attttctcaa tgttctttcc gtgtctcaaa tatcttaaca tttctgactt
    10801 tattggcaag tcaggctgtt attttagcca gtcctgttat gatagtattg ttactatcag
    10861 aagtattact tggtgtatta tcgagatttg ctccgcagat gaatgctttt tccgtatcat
    10921 taactattaa aagtttactt gcaatattta ttattttcat ctgttcttct actatttact
    10981 tttctaaagt tcaatttttc ctcggtgaac ataaattttt cacaaatcta tttgttagat
    11041 aaaatattat ggcaaataaa acagaaaagc cgacacctaa aaaactaaag gatgccgcaa
    11101 aaaaaggaca gtcatttaaa tttaaggatt taacgactgt tgttattatt ctggtaggga
    11161 catttactat aatatcattc ttttccttaa gtgatgtaat gctcttatac agatatgtaa
    11221 taattaatga cttcgaaatt aatgagggta aatacttttt tgcagtggtt attgtctttt
    11281 ttaagataat tggcttccca ctttttttct gtgttctttc ggctgtgttg ccaacattgg
    11341 ttcaaacaaa gtttgttctt gcgactaaag ctatcaagat tgatttttca gtattaaacc
    11401 ctgttaaagg gttaaaaaaa atatttagta taaagacaat aaaagaattt ttcaaaagca
    11461 ttctgcttct tattattcta gcattaacaa cctatttctt ttggattaat gaccgaaaaa
    11521 taattttttc tcaggtgttt tctagtgttg atggcttata tcttatttgg gggaggctgt
    11581 ttaaggatat aatattattt ttcttggcat tttctgtttt tgttattatt cttgactttg
    11641 tgattgagtt cattttatac atgaaagata tgatgatgga taaacaggag ataaaaagag
    11701 aatatataga gcaagaggga cactttgaga caaagtcgag aaggcgtgag ttgcatatcg
    11761 agattctttc agagcagact aaatctgata tacgtaattc aaaattagtg gtaatgaacc
    11821 cgactcatat tgcaattggt atttatttta atccagaaat agcgcctgca ccttttattt
    11881 ctctcattga aactaaccag tgtgccttgg ctgtcagaaa atatgcaaat gaagttggta
    11941 taccgactgt gcgtgatgtg aaattagcta gaaaactata taaaacacat acaaaatata
    12001 gttttgttga ttttgaacac ttggatgaag tcctacgtct tattgtttgg cttgagcagg
    12061 ttgaaaacac tcattaataa gtaaaggaga tgtgttatat gggagttaat ttctgtaata
    12121 aaataggtat tgatcagtct gaatttgaaa tagaatcttc catcattaac tccattgcta
    12181 atgaggtatt gaacccaata tctttcctta gcaataagga tataataaat gttttactca
    12241 gaaagatttc atctgaatgt gacctggtta gaaaagacat ttatcgttgt gctctggagt
    12301 tggtcgttga aaaaacacct gatgatttat aatcattcat aataactctt ttgctgatat
    12361 aaatattttt gttgaagata tttatgtagt cacaggatca attgtagaaa attgttatgt
    12421 ttttgttagg aactgcacta aataatatat gataagacag caaaaacgac tgaccataat
    12481 ccttctttta ttaggagttg ataagagaga ctattcatcc tgtaatgtta aaactctgtt
    12541 atatagtatt cgcgattatg caaagtctgt aaatgatcat gagatactaa ccgagagtaa
    12601 tcgtttatta tctcattgca tttcagatag taacggagct ttctttaaaa gtagtaaata
    12661 cgttcccctt aagtacttac gtaaaaggag aatagcacga aagataccaa atgattagca
    12721 ctttgctagt aagcttgctg gtaaactata ttgaccgttg ggtttgtttt gagactgtaa
    12781 tttaaattgt gtaattgcct gtttttgata tgtttactcc aacaacggag acaggtaaat
    12841 tatggacgaa aagaagctta aagcccttgc ggctgaactg gctaaaggcc tcaaaactga
    12901 ggccgacctc aatcagtttt cccgtatgct gacgaaatat cccggct
//
LOCUS       DAJTRQ010000112        12943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000112 DAJTRQ010000000
VERSION     DAJTRQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..462
                     /locus_tag="OO332_003198"
     CDS             <1..462
                     /locus_tag="OO332_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5943575.1"
                     /translation="GNTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNL
                     PAIKLNTTRRRAGEGLRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLR
                     KNRLPVEVVKIPMSGPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            459..858
                     /locus_tag="OO332_003199"
                     /pseudo
     CDS             459..858
                     /locus_tag="OO332_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            869..1609
                     /locus_tag="OO332_003200"
     CDS             869..1609
                     /locus_tag="OO332_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5943576.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1668..2054
                     /locus_tag="OO332_003201"
     CDS             1668..2054
                     /locus_tag="OO332_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5943577.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2063..2392
                     /locus_tag="OO332_003202"
     CDS             2063..2392
                     /locus_tag="OO332_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5943578.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2364..5303
                     /locus_tag="OO332_003203"
                     /pseudo
     CDS             2364..5303
                     /locus_tag="OO332_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5303..5632
                     /locus_tag="OO332_003204"
     CDS             5303..5632
                     /locus_tag="OO332_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5943579.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5632..6330
                     /locus_tag="OO332_003205"
     CDS             5632..6330
                     /locus_tag="OO332_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5943580.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6335..7078
                     /locus_tag="OO332_003206"
     CDS             6335..7078
                     /locus_tag="OO332_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5943581.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7015..7616
                     /locus_tag="OO332_003207"
                     /pseudo
     CDS             7015..7616
                     /locus_tag="OO332_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            7677..11156
                     /locus_tag="OO332_003208"
     CDS             7677..11156
                     /locus_tag="OO332_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5943582.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            11224..11823
                     /locus_tag="OO332_003209"
     CDS             11224..11823
                     /locus_tag="OO332_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5943583.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11887..>12943
                     /locus_tag="OO332_003210"
     CDS             11887..>12943
                     /locus_tag="OO332_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5943584.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     AIAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLCIRWTDARPETTEPRYRAKSLTFYGI
                     NGPIYHTRYCYWPISRLT"
BASE COUNT         3055 a         3090 c         4113 g         2685 t
ORIGIN      
        1 gggaataccg ttgcgggcga taaccaggtg aaagggatcc ccctgaaact ggtacgtcag
       61 cgtgtccggg tgtttaaagc cagtccgtca ggaaaaatga cggccaggat ccgcgttaac
      121 cggggcaatc tgcccgccat caaactgaac acaacacggc ggcgtgctgg tgaaggactg
      181 agagtgggaa aatacttttt ccggggggca tttgttcagc aactggcgaa tggccgctgg
      241 catgttctga ggtgtcttcc tgaagcgcgt tttgcaacag ggcatgacca tcagggcagg
      301 ctaagaaaaa atcgtcttcc tgtggaggta gtgaaaatcc cgatgtccgg accgctgaca
      361 caggcatttg aagatgcccg cgatcgcatc attgctgcgg aaatgccgaa acagctgggg
      421 tatgccctga aacaacaact gaggttacat ctgagtaaat gaaccggcac acacaaatcc
      481 gtcagagcgt gctggcacgc cttcgggaac agtgtggaga cagcgccacg ttttttgacg
      541 ggcttccggc atttattgat gcgcaaactg cctgccgtgg cggtgtggct gagtgatgct
      601 cagtacaccg gaaaaatgac ggatggagat gactggcagg ctgttctgta tattgccgtc
      661 ttcatccggg cacaggcacc ggattcagag ctggatatgt ggatggagag caccattttc
      721 ccggccctga atgatgtacc ggcactttcc ggactcatcg acaccctgaa cccactcggt
      781 tttaactatc aacgtgataa tgagatggct acctgggcga tggcggaaat cacgtaccag
      841 atcacgtaca cgaattaagg aggtggtaat gaccacacca aatccactgg caaaaacgaa
      901 aggtgcggga acgacgttat ggatgtatac cggcaacggc gacgcatttg cgaacccttt
      961 gtcggacact gactggctgc gtcttgcgat ggtgaaggat ctgcaacctg gcgagatgac
     1021 cgctgatgca gaagatgaca cttatctcga tgatgaagat gcagactgga aaacgacaac
     1081 ccaggggcag aaatccgtcg gtgatacttc ggcgacgctg gcctggcgtc cgggtgacag
     1141 cgggcagaaa aaactggttc agttgttcga ttccggtgaa gtctgcgcgt ttcgtatcaa
     1201 ataccccaac ggtacggttg atgttttccg tggctggctg agttcactgg gtaaaaccat
     1261 tacctcaaaa gaggtgatga cccgtactgt aaaaatcagc ggtgtggggc gtccgtatct
     1321 ggcagaggag ggcgctgaaa ccgtgggtgt taccggactg acggtggcac cggcatctgt
     1381 cagtgtcaaa gcgggagcaa ccaccacact gacctttaca gtaaaacctg acggggccag
     1441 tgacaaagcg atcagtgtgc attcgtcaga tcctcagact gcttcggtgc ccctgagcgg
     1501 gcttgtggcc acggtgaaag gcgtgcagca gggcagtgtc agcatcgtgg gcatgacctc
     1561 tgacggagag tttgtggcag tggctgcggt gaccgtcagc gcaccataac aggacgatac
     1621 tcatcattgc cccggttatc cggggctttt tgcatccgga gaacatgatg tttctgaaac
     1681 aggatacgtt taattatgaa aaacagtccg tggtgctcag tgagctgtcc gggctgcaga
     1741 gaattgaata tctgacgttt gttcagcagc gaaaggcaaa gtttgatgca caggagggag
     1801 aactgccgga ggctgaacga cagattgctt ttctgcgtat gggaatggat atcaatgcct
     1861 ggctggtttc ccgctcactg tggaatgctg agcagtctca ggatgttgag acgctttgcg
     1921 catccattat gacaacatgg tcgtatgatg cgctgggcgc gggggcggag agggttctgt
     1981 cgctgagcgg tatggggacc attgagaatg ccggggatga tgatcatgag gcgctgacgc
     2041 cggaaaagtc ctgacgcggg aaatgcagtt tgtcatgcgg cttgcccggg agttccggcg
     2101 ggcagactgg cggcagatgc tgtcggatat gtcggccact gagcttggtg agtggggcga
     2161 ttatttccgg atgcagagct tcagtgatgt gtggatggat gcgcagtttg cctcgctgaa
     2221 ggcattgatc gtgagaatgg tgtccggcag cagcgatgct gcggtggctg atttcagcct
     2281 tttaccggaa gagaacggga taccggagcg aacggacgaa gaactgatgc atcttgggga
     2341 aggtatttcc ggaggtgtgc gttatggacc agatagccaa cctggtcatt gatttgggga
     2401 ttgatgcggc agagtttaaa aatgaaattc cccgtatcaa aaaccttctg aatggtgcag
     2461 ccagcgatgc agaacggtct tctgcccgta tgcagcgttt tatggagcgt cagactcagg
     2521 cggcccggca gacaatgcag gcggcttctt cggctgcaac agcagcatca gcccatgcgc
     2581 agacggtgga gaagaatgca cgggctcatg aacgcatggc ccgtgaggtg gaacaaaccc
     2641 gtctgcgcgt ggatgccctg aatcagaaaa tgcgcgagga acaggcgcag gccagggcac
     2701 tggcggaggc gcaggataaa gcggctgccg ccttttatcg ccagattgac agtgtgaaac
     2761 aggccggtgc ggggcttcag gaattacagc gtattcagca gcagatccga caggccagaa
     2821 acagtggcgg ggttggtcag caggattatc tggcgctgat ttcggagatc acggcgaaaa
     2881 cccgtgccct gacgcaggca gaggaacagg ccacccggca gaaagcggcg tttatccgcc
     2941 agcttaaaga gcaggcaacc cgccagaatc tgtcgtcttc tgagttgctt cgcgccaggg
     3001 cggctcagct gggggtgtta atcagtcaga tggcgcgcgg caattttggt gcgctgaggg
     3061 gatccgggat aacgctggct aaccgtgccg gatggacagg cgcactgatg tcgccgaaag
     3121 gcatgatgac tggcggcgtt attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag
     3181 cctggcatga cggcggaagg agggcgagga atttaaccgt cagctggcgc tgacgggaca
     3241 ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat ccgggaatgg
     3301 tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg gggcatttca
     3361 tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat cggttggcca
     3421 gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg taaatgccgc
     3481 gaaggcgctg gacaatgcgc tgcatttttt gactgccact cagcttgagc agatacgcgt
     3541 ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg cgctggcaga
     3601 ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg gtagtacgct
     3661 gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata ttggtcgtga
     3721 agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg cgaaaaaata
     3781 tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta acgatcttca
     3841 ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac ggaattacca
     3901 ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga atgaaacgga
     3961 agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg ccgaccaggc
     4021 tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca ttaagaaaaa
     4081 tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca gtcagcagca
     4141 ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg ccactgaaag
     4201 gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg acctggacgg
     4261 gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc tgattcaggc
     4321 gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc ttaacgacct
     4381 gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg cactgcgtga
     4441 gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc agcgctacca
     4501 ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg agaatgacag
     4561 tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg tgctgaaggg
     4621 gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg aagtggcgca
     4681 gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg atgcggataa
     4741 tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg gtaatgcact
     4801 ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt ccctgttgtc
     4861 agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg gcgtcggttc
     4921 tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg atttgagtcg
     4981 ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag gcgcgggtgt
     5041 gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg atggtaagct
     5101 ggggggttgt ggcggatact ggtggttcag gcatggtgat gtttgccccg cagtacaaca
     5161 ttgagatcaa taacgacggc acgaacgggc agataggtcc ggctgccctg aaggtggttt
     5221 atgacctcgg gaaaaaagcg gccgcggact ttatgcaaca gcaggcccgt gatggtggcc
     5281 ggttaagtgg agcataccgg taatggagac gtttcactgg aaagtgcgcc cggatatgaa
     5341 tgtggtatca gagccgaaag tggtgacagt gaagctgggc gatggttatg aacagcgtcg
     5401 tgcggcggga ctgaataacc agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg
     5461 tgaacaccca tctttaaaag cctttctgga acggcacggt ggcgtccgcg catttcagtg
     5521 gacgccacct tatgactgga aactgatcag ggaggtttgt cgtaaatggt cggcaagcgt
     5581 gggggcgtta tgggtgactg taacggccga ttttgaacag gtggtgaact gatgcaggat
     5641 atccggcagg aaacactgaa tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc
     5701 tgggaaatcg atctgacaga ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac
     5761 gaaaaaggtg agccggtcac ctggcagggg cggcagtatc aggcataccc cattcagggg
     5821 gcggggtttg aactgaacgg caggggctgt gctgcccgtc cgacactgac ggtctctaac
     5881 ctgcacggca tggtcaccgg gatggcggaa gatctgcaga gtctggtcgg cggaacggtg
     5941 gtccggcgta aggtttacga ccgttttctg gatgcggtga acttcgtcaa cggaaacagc
     6001 gacgccgatc cggaacagga ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg
     6061 agcgcggtca gtgcctcttt tgtgctggcc acaccaacgg agacggatgg cgcggttttc
     6121 ccggggcgta tcatgctggc gaatacctgt atgtggactt accgttctga tgagtgtggt
     6181 tacacgggca gggcagtggc tgacgagttc gacaaaccaa cgacggatat ccggaaggac
     6241 aaatgcagca agtgtatgcg cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt
     6301 ttcctttcca tcaataaact ttctcagtaa atccatgaca cagacagaat cagcgattct
     6361 ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgtgga ttcgtggtga gaacgccgga
     6421 gggagacagg tatcttccca gcgagaatat ctccggtgag ccggaggaac ggttccggat
     6481 ggctccggag gactggctgc gggcacaaat gcagggtgag attgtggcac tggtccacag
     6541 tcatcccggt ggtctgccct ggctgagtga ggctgaccgg cggctgcagg tgcagagtga
     6601 tttgccgtgg tggctggtct gccggggggc gattcacaag ttccgctgtg tgccgcatct
     6661 caccgggcgg cgctttgagc acggggtgac ggactgttac acgctgttcc gggatgctta
     6721 tcatctggcg gggattgaga tgccggattt tcaccgcggg gatgactggt ggcgtcacgg
     6781 tcagaatctc tatctggata atctggaggc cacagggctg tatcaggtgc cgttgtcatc
     6841 agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca tcggtgccga atcatgccgc
     6901 catttactgc ggcgatggtg agctgctgca ccatattcct gaacaactga gtaaacgaga
     6961 gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc gggaatggca
     7021 cgcatctgcc tttacgggga tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa
     7081 acgggggctg aagccatccg cgcactggcc acacagctcc cggcgtttcg tcagaaactg
     7141 agtgacggct ggtatcaggt gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct
     7201 gcccgtctta atgagccgct ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg
     7261 ggagctaaaa gtggcggtgt gtttcaggcg gtgctggggg cagctgttat ggcggttgct
     7321 atatggatgc cggggtagga attatggcga gtaatctgct gttttctctc ggtgccagta
     7381 tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc cgtacacaga
     7441 caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg gttgcccagg
     7501 gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg gtatctcagg
     7561 agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt cgctgatgca
     7621 aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg tgaggaatgg
     7681 gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg aaatccacgc
     7741 agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg gtggatggat
     7801 taaaaagcgt gctgctgaac ggtacgccgg tcctggacag cgaggggaag acaaactttt
     7861 ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg gaggggtttg
     7921 aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg ccgatcaccc
     7981 ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg caggcactgg
     8041 tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg gttcagatac
     8101 agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa accacttcgc
     8161 agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat atccggatgc
     8221 gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc tggtcgtcgt
     8281 acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc ggcgtgcagg
     8341 tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt cgcgggcgca
     8401 ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt atctgggacg
     8461 gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac atgctcactc
     8521 atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa tgggcgctgt
     8581 atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc acggagccgc
     8641 gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt ctcagtgatt
     8701 tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg ttcgtgcagg
     8761 accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg ccggatgatg
     8821 gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc gttgaggtga
     8881 actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag gacacgcagg
     8941 ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt accagccggg
     9001 gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa acgcagaccg
     9061 tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc attgaaatct
     9121 gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg aacagccaga
     9181 cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc acgctgataa
     9241 gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc accgacggcg
     9301 tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg tgggggctga
     9361 agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag aacgacgacg
     9421 gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc gtggataacg
     9481 gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg ccagcggtgc
     9541 agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg gagcgatggg
     9601 acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg gcagcggacg
     9661 acggcagtga gcggctggtc agcacaggca ggacgacgga aaccacatac cgcttcaggc
     9721 agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg gggcagcagg
     9781 gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc actattgaac
     9841 tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat gacccgacgg
     9901 tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag gttgaaacca
     9961 gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat atcaggccgg
    10021 gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg gcatttgtgg
    10081 aggccgtcgg tcgggcgagc gattatgcgg aaggttatct gagtttttat aaagggttga
    10141 tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt cagcttgcgc
    10201 cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc acgcaaaccg
    10261 tcaataaaac actggaaaat cagagtgctg caatccagca gatacagaaa gttcaggttg
    10321 atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg aaggacggac
    10381 gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga atgcagagtc
    10441 aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc aacacaaagc
    10501 cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg aaacgcctga
    10561 cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca cctggcggac
    10621 ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc gggacgctca
    10681 acaacgtcac gactaataag aactgtcggg ttctgggaaa attgtccgcg aaccagattg
    10741 aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg gcaccggaga
    10801 ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt gatcggcaaa
    10861 ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat aacaatattt
    10921 attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac cgaacgaccc
    10981 tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac ggtcacatga
    11041 cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca gcaagtatca
    11101 gcgatttgct ggttgttgtg atgaagaaag ccactgcagg catcacgatt agctgaattt
    11161 tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa gcgagaggta
    11221 attatgcgta aagtttgtgc agccattttg tccgcagcca tctgtctggc tgtatccggt
    11281 gcgcctgcat gggcgtctga acatcagtcc acgctgagcg ccgggtatct tcagccccat
    11341 actgatatgc caggcagcga tgacctgaag ggcattaacg tgaaataccg ttatgagttt
    11401 acggacacgc tggggctggt gacgtcattc agctatgcca acgctgaaga tgagcaaaaa
    11461 acgcattaca gcgatacccg ctggcatgag gattccgtgc gtaaccgctg gttcagcgtg
    11521 atggcggggc cgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat ggcgggcgtg
    11581 gcttacagcc gtgtgtcgac cttctccggg gattatctcc gcgtaactga caacaagggg
    11641 aaaacgcacg atgtgctgac cgggagtgat gacaaccgcc acagcaacac gtctctggcg
    11701 tggggggctg gtgtgcagtt taacccgacc gaatccgtgg ccattgacct tgcttatgaa
    11761 ggttccggca gtggcgactg gcgcactgac ggtttcattg tgggtgtcgg ttataagttc
    11821 tgattagcca ggtaacacag tgttatgaca gcccgctggt tcaggcgggc tttttgtggg
    11881 gtgaatatgg cagtaaagat ttcaggtgta ctgaaagatg gggcgggtaa acctgtcgta
    11941 aattgtgcga ttgaactgcg ggccagaaga accagtccga ccgttgtggc acacgttgtt
    12001 gccacttgcg tgacggacaa taacggtgct tatgtgattg aggctgagcc gtggtattac
    12061 gaggttgcgc ttcactgtaa cggctggcag ccaacccgtg tcggggatat tgatgtggca
    12121 ccgactgatg caccggggac actgaacgcg tttctgaatg caccaaagga tggtgattta
    12181 cgtccggagg tgatgaagcg ctttgaggaa atggtggcgc aggcgcagca gagtgccggg
    12241 gctgcagccg gaaacgcaca gcagacggcg caggatgtgg cggcagccgc aacggcccgt
    12301 gatgatgcac aacgttttgc ggagaaagcc cgacaggatg caaccgtcac agctgaggac
    12361 agaaaggcca ttgcggaaga tgtgacaagc acaggagcaa atgcagccgc agccggacag
    12421 agcgcacagg atgccgcagg ttatgcccgc gcagcagaac aggccaaaaa tgacattgat
    12481 gctgcgctga ccggcaccct gaaaacggct aaccatctgt ctgaaatcgc agcagcaggc
    12541 gaaaaggcac aacagaagtc ccgggataat ctggggctga aaagtgcggc cacgatggaa
    12601 gcacagagcg acatttacga ccggacaaaa ggccgtctgg cgatacccgg cgcattcggc
    12661 tttgggtgtg cttttctgcc tgaagatgtt atccgttttg acactaagag tgatttcctg
    12721 gcctgggtaa ggaatgcgct gccaggtgaa tattccgttg ctggccctta cggcatcatc
    12781 atacccgaca cacggtttga aggggtgctc tgcatccggt ggactgatgc acgccctgag
    12841 acaacagaac cgcggtacag agccaaatcc cttacttttt acggcattaa cggccccatt
    12901 tatcataccc gctactgcta ctggcccata tccagactga ctg
//
LOCUS       DAJTRQ010000113        12800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000113 DAJTRQ010000000
VERSION     DAJTRQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(308..1399)
                     /gene="ychF"
                     /locus_tag="OO332_003211"
     CDS             complement(308..1399)
                     /gene="ychF"
                     /locus_tag="OO332_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5943585.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1516..2100)
                     /gene="pth"
                     /locus_tag="OO332_003212"
     CDS             complement(1516..2100)
                     /gene="pth"
                     /locus_tag="OO332_003212"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5943586.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2378..2656
                     /gene="ychH"
                     /locus_tag="OO332_003213"
     CDS             2378..2656
                     /gene="ychH"
                     /locus_tag="OO332_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5943587.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2711..4390)
                     /gene="dauA"
                     /locus_tag="OO332_003214"
     CDS             complement(2711..4390)
                     /gene="dauA"
                     /locus_tag="OO332_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5943588.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4515..5462)
                     /gene="prs"
                     /locus_tag="OO332_003215"
     CDS             complement(4515..5462)
                     /gene="prs"
                     /locus_tag="OO332_003215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5943589.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5613..6464)
                     /gene="ispE"
                     /locus_tag="OO332_003216"
     CDS             complement(5613..6464)
                     /gene="ispE"
                     /locus_tag="OO332_003216"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5943590.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6464..7087)
                     /gene="lolB"
                     /locus_tag="OO332_003217"
     CDS             complement(6464..7087)
                     /gene="lolB"
                     /locus_tag="OO332_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5943591.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7301..8557
                     /gene="hemA"
                     /locus_tag="OO332_003218"
     CDS             7301..8557
                     /gene="hemA"
                     /locus_tag="OO332_003218"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5943592.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8599..9681
                     /gene="prfA"
                     /locus_tag="OO332_003219"
     CDS             8599..9681
                     /gene="prfA"
                     /locus_tag="OO332_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5943593.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9681..10514
                     /gene="prmC"
                     /locus_tag="OO332_003220"
     CDS             9681..10514
                     /gene="prmC"
                     /locus_tag="OO332_003220"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5943594.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10511..10903
                     /locus_tag="OO332_003221"
     CDS             10511..10903
                     /locus_tag="OO332_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5943595.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10907..11716
                     /locus_tag="OO332_003222"
     CDS             10907..11716
                     /locus_tag="OO332_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5943596.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11752..12606
                     /gene="kdsA"
                     /locus_tag="OO332_003223"
     CDS             11752..12606
                     /gene="kdsA"
                     /locus_tag="OO332_003223"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5943597.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12755..>12800)
                     /locus_tag="OO332_003224"
     CDS             complement(12755..>12800)
                     /locus_tag="OO332_003224"
                     /inference="COORDINATES: protein motif:HMM:NF025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5943598.1"
                     /translation="IITAAIVGWWRNRK"
BASE COUNT         3190 a         3237 c         3327 g         3046 t
ORIGIN      
        1 cccttcaggt aagtcctttt ctcgccagca tattggagcg agatcaatat tgatcagctg
       61 tgaagagggc tgatgtgtag tggaacaaaa actgacgtta tatcactgct aatgagatat
      121 cagtcatctg aacacttatg tctttaatta ttctcgtggt tcactgtagg caataagcac
      181 aaaagtgtag gatgttacaa gaatgattag gactcggtga aatgaaaaat ccacgcaatt
      241 gcgtggattt tatatacttt tgcgctcttc atgagattta gcgaaacctc atgagacaat
      301 aaattaatta gacgttgaaa aggaagttca tcacatcgcc atctttcacg atgtaatctt
      361 taccttctgc acgcattttg cctgcttctt tcgcgccttg ttcacctttg taagtgatga
      421 aatcttcaaa cgagatggtt tgtgcacgga taaagccttt ttcaaaatca gtatggattt
      481 tgcccgctgc ctgcggcgcg gttgctccaa ccggaatggt ccatgcacgc acttctttca
      541 ccccagcggt gaagtaagtt tgcaggttca gcagcttata accggcacgg atcacacggt
      601 tcaggcccgg ttcttccagc ccaagctcct gcataaactc gtcacgttct tcgtcgtcca
      661 gttcggcaat gtctgcttca acagcagcac aaaccggaac cacaacagaa ccttctttcg
      721 ccgcgatttc acgcacctgg tcaagatatg ggttgttttc aaaaccgtct tcgttgacgt
      781 tggctatgta cattgttggt ttcagcgtca ggaagctcag gtaacgaata gccgctttct
      841 cttcagcgct taaatccagc gcgcgcagca tacctgcgtt ttccagctgg ggcaggcatt
      901 tttccaggac cgccagctca gctttcgcgt ctttatcgcc acctttggct ttcttctgta
      961 cgcgatgaat cgcacgttcg caggtgtcga ggtctgccag cgccagttcg gtgttgataa
     1021 cttcaatatc gtcagccggg ttaactttgc cggaaacgtg aatgatgttg tcattttcaa
     1081 agcagcgaac aacgtgaccg atcgcttcgg tttcacggat gttggtcagg aactggttac
     1141 ccagaccttc gcctttcgat gcgcctttta ccagaccggc gatatcgaca aattccatgg
     1201 tcgtgggaag cgtacgttgc ggttttacga tttcagccag ttgatccagg cgagggtcag
     1261 gcattggtac gacgcctgtg ttcggctcaa tggtgcagaa tggaaagttg gccgcttcaa
     1321 taccggcttt ggtcagcgcg ttgaacaggg tagatttccc gacgttgggc aaaccgacga
     1381 taccgcattt gaatcccatg atttaaatca ccttaatatc ttaataatca acctgttatt
     1441 gctaacagat tgcagaaatg gaaataactt tgcctattat acacggcact cggcaaaaat
     1501 gccgcagaca acgacttatt gcgctttaaa ggcgtgcaat cggttcgttg ctttggtcaa
     1561 gccatctgta aaccacattt cagtacaacg cgccgcttcg tcgatggctt catcaattaa
     1621 cttctgttca ctaacaggcg gtttgcctaa cacaaaaccg acaactttat ttttatcgcc
     1681 cggatgaccg attccgatgc gtaaacggtg aaagttaggg ttattaccca atttactgat
     1741 gatgtctttc agtccattgt gaccaccatg gccaccgccc aacttaaatt tggcgacgcc
     1801 aggaggcaga tccagttcgt cgtgggccac cagaatttcg tccggattaa tgcggaaaaa
     1861 actggccatc gccgcaacgg ctttgccgct gagattcata aatgtagtcg ggactaacag
     1921 gcggacatct tcgcctccaa gggtgactcg cgaagtataa ccaaagaatt tagcctcttc
     1981 gcgcagcgga gcgcgcaaac gctctgccag taagtcaacg aaccaggcac cagcattatg
     2041 tcgcgttgcg gcgtattcag caccggggtt cgccaggccg acaatcaatt taatcgtcac
     2101 gtttttttgt cctgagtgtg tacataactg gcgcgtagtt tactggttgc ggccccgctt
     2161 gacaaaaaac tgcgtatcaa atgcagataa cgtaataatt gcctgagtgg actattagaa
     2221 agtcaaggtg ttcaggcgtt tatttgtaaa gttttgttga aataagggtt gtaattgtga
     2281 ttacgcccgc acataaccca cagggtgttg tctatacttt acacataagg aagaggggta
     2341 ttccctgtta caacccagaa agttccggag gtgacatatg aaacgcaaaa acgcttcgat
     2401 actcggtaac gtgctcatgg ggttgggtct ggtggtaatg gtggtcggcg tggggtattc
     2461 aatcctcaac cagttaccac agtttaatat gccccagtat ttcgcacatg gtgcagtgct
     2521 aagtattttc gtcggtgcca ttctctggct ggcgggtgcc cgtgttggcg ggcatgaaca
     2581 ggtgtgcgac cgttactggt gggttcgtca ctatgacaaa cgttgccgcc gtagcgataa
     2641 tcgccgtcat agctaacaaa taatgcagtt tgctgaccag tcagtttgcg ctgactggtc
     2701 aatctcgtac ttataaatcc gccatcgccg cgcgacgatt cgggaagaac gcgaggcgtc
     2761 ccgggatcgg ttgaatgcct gcgcgagcca tagttcgcag tggctggaat tccacgttgc
     2821 acacgcgcag ttcacatcct tcgggcagac gcttcacaaa acgctggaac gcatcaagac
     2881 caccagcatc aagtaccgga acggcatccc acttcagaat cacaatccgt ttgccttcaa
     2941 gacgtgactc caggtccgtg aataagcctt cagcagcagc aaaaaacagc gggccaataa
     3001 cgcgcagaac caggacatcg tctggaacat ctacgactac cggtgccagg cgagtcatac
     3061 gtgcgatacg acgcataaac agcagcgatg ccagcacgat ccccacgctg atggcaataa
     3121 ccatatcaaa cagcacggtc agtgacatac acagcagcat gacgatgatg tcatctttcg
     3181 gcgcatgacg cagcaagtcg accactttgt gcgcttcact catgttccac gccaccatca
     3241 acagcagggc tgccatggcg gaaagcggca gccaggagag cagtggtgcc agtaccagca
     3301 gggcgagaat aaccagaata gagtggatca ccgccgagat aggggaagtt gccccggcac
     3361 ggacgttagc ggcagaacgc gcgatggcag cagtagcggt aataccacca aagaatggag
     3421 cgatgatatt ccccagcccc tgaccgacca gttcactatt cgccttgtgc ttagtcccgg
     3481 tcataccatc cagtaccaca gcgcagagca gagattcgat cgccccgagc attgccattg
     3541 agaatgccgc aggcagcagg gtgcgaatag aatcccaggt tagcgtgaat tctgaattag
     3601 gcagatccca cggcagcacc agttgcggca gcagttgcgg aataccgtta ccctgagaac
     3661 catcggccag gacgtagtgg aattgcgaac cgatggtagc aacatgtccg ccgagcaggt
     3721 taacaatccc catcaccgcg caaccagcca gcaaggccgg aaggtgaccg ggtaaacgaa
     3781 tgcccagacg cggccagaaa accagaatgc caagcgtcac aatgccaatg gcagcatcac
     3841 ccacattaat ggtcggcagc gccataaata atgcgccgac tttttgtaga taatgttccg
     3901 ggacatgggc catttgcaga ccgagaaaat ctttaatctg catggtaccg atggtgatcc
     3961 cgatacccga ggtgaaacct aaggtgacgg aaaccggaat atactcaatc agacgaccaa
     4021 agcgtgccag ccccatcaga atcaaaaaga ttcccgacaa caaggtcgca accagcagtc
     4081 ctgccagtcc aaactgttgc gaaacgggat agagaattac cacaaatgcc gcagtcggac
     4141 cggaaacgct aaagcgtgac ccacccgtta gagcaatgac aatccccgca acagctgcgg
     4201 tatataaacc gtactggggt gccacaccac taccaatagc caacgccatc gccagcggga
     4261 tagcaataat cccgacggtt atcccggcaa tcaggtcacg ggtaaaccgt gcggcagtat
     4321 atttttcttt ccagcaagcg tcgatcagag cgcggaaagg catcacatgt gaggaaaata
     4381 ttttgttcac aataatgttt catccgtgag cgcatcatct gtcaactaaa tggcaggtga
     4441 aggaggcata ggtcatacaa atggatatta cagacaaaaa aacccgccgc agcgggtctt
     4501 tgagccgggt tcgattagtg ttcgaacatg gcagagatcg attcttcgtt gctgatacga
     4561 cgaatcgctt cggccagcat acctgacagg gtcagagtac gcacgttcgg cagtgatttg
     4621 atttcatcgc tcagcggaat ggtatcgcag acaacgactt catcaattac agagttacgc
     4681 aggttgttcg ccgcgttgcc agagaagatc gggtgagtcg cgtacgcaaa tacacgttta
     4741 gcaccacgtt ctttcagcgc ttcagcagct ttacacagcg taccgccagt gtcgatcata
     4801 tcatcgacca gtacgcagtc acgacctgca acgtcaccga tgatatgcat cacctgggaa
     4861 acgttcgcac gcggacgacg tttgtcgatg attgccatat cggtatcgtt cagcagctta
     4921 gcgatagcgc gggcacgcac aacgccgccg atgtccggag aaaccacaat tgggttatcc
     4981 agattcagct gcagcatgtc ttccagcagg atcgggctac caaatacgtt atcaaccgga
     5041 acgtcgaaga aaccctgaat ctgttcagcg tgcagatcca ctgtcagcac acggtcaaca
     5101 ccgacgctgg agaggaagtc tgcaaccact ttcgcagtga ttggtacacg agcggaacgg
     5161 acgcgacggt cctggcgcgc atagccaaag taggggataa cagcggtgat acgacctgcg
     5221 gaagcacgac gcagggcatc aaccataacg actaattcca tcaggttgtc gttagtaggg
     5281 gcacaagtgg actggatgat gaaaatatca ccaccgcgta cattttcgtt aatttgtacg
     5341 ctgacttcgc cgtcgctaaa gcgacctaca gcggcgtcgc cgagtgaagt gtacaggcgg
     5401 ttggcaatac gttgtgctag taccggggtg gcgttaccag caaaaagctt catatcaggc
     5461 acgagaagaa cctcaggcat gcgtccattg gtggaaagaa tctgccgaaa actgtgcggg
     5521 ccaggcatga tcctttccag gcggtgtatt aaagagcgcg atgcaacgtc tggaacaagg
     5581 tgacgttgtc accgaaactc agcttgcccg gcttaaagca tggctctgtg caatggggaa
     5641 agattaacgc ctttcgccac aaagccattg agccattccg gggcttgctc tagcacctgg
     5701 cgggcttcag actctgtatc aaattcagca aagacacagg cccctgtccc agtcaggcgc
     5761 gacggggcgt attttaacag ccaggaaagc accgcatcaa cctcgcgaaa acgttttctt
     5821 gcgataacct cgcaatcatt gctgaattca cattttagca acgtttctat tgaccttttt
     5881 ggcgtattgc gcgggagttc aggatcttta aaaatcaccg gagtcggaat acttacacca
     5941 gggtgcgcca ccagatacca cttctctggc ggatccaccg gcgttagtat ttcaccaacg
     6001 ccttcggcaa acgcggcatg cccccgaaca aagacaggaa catctgcgcc cagcgtcagc
     6061 cccatttccg ccagctcatc catgcttagc ccgcattgcc agagatgatt taatgccacc
     6121 aggaccgtcg cggcattgga tgaaccaccg ccgagaccgc cgcccatcgg caaacgcttg
     6181 tcaatgctga tattcgcacc gcttcccgtc ggaagacgcc cgctgtctgc cgcagttttc
     6241 atcaacaacc gcgctgcgcg aacgatcagg ttatcttcat gttccacgcc ttcaacgggc
     6301 gttaacagac gaatatcccc atcgtcacga agctcaatgc tgatggtgtc gccgtaatca
     6361 agaaactgaa acagcgtttg cagcgtgtgg taaccatccg cacgctgacc ggtaatgtat
     6421 aaaaacagat taagttttgc cggagagggc cactgtgtcc gcattatttc actatccagt
     6481 tatccatttt taacttgatg cgttgaccac cgtcggtgag ttccatattg gttggcatcg
     6541 caggttgcgt tttggtgtca taaccaccat aaacaacctt ccagtttttg ccattctggc
     6601 tgtaggtaat ttcgctcagg cggtactggt cgtccagttt gtagtcggtt gcatcacccg
     6661 gtaaacctaa aatccactgg cgcaagctgt tgagcggaat tggcattccg gtcaatttgc
     6721 caatcatctc ttcggcgtca tcggcggtat aacgctgacc tttattgtcg actaactgca
     6781 cgttacccgg ttgagcattc agctccagtt ccgtgctgcc caatgggtta gtgagcagca
     6841 gacggtagcg atcctggccg gtttgctgcc agaaaaagcg ggcgtacact ttttgttggt
     6901 cagaaatata agcgaacgcg ccgcgagtct gatactgatt aagattgcgc acgtcttgct
     6961 gatgctgacg ccattgtggc gaatccgggc ttttgccagg acctttgggc gtggtaacgg
     7021 aacaggcagt gagcacaaga gcagccagcg gtagcaggcg gataagacga aaatcgggca
     7081 ggggcatagt gatgacaagt ccttgagata cgttgcagtt ataaccctta atgctagcgt
     7141 taccgtccgc tatcgtctat gttcaagttg tcttaattgc cagaatctaa cggctttcgg
     7201 caattactcc aaaagggggc gctctctttt attgatctta cgcatcctgt atgatgcaag
     7261 cagactaacc ctatcaacgt tggtattatt tcccgcagac atgacccttt tagcactcgg
     7321 tatcaaccat aaaacggcac ctgtatcgct gcgagaacgt gtatcgtttt cgccggataa
     7381 gctcgatcag gcgcttgaca gcctgcttgc gcagccgatg gtgcagggcg gcgtggtgct
     7441 gtcgacgtgc aaccgcacgg aactttatct tagcgttgaa gagcaggata acctgcaaga
     7501 ggcgttaatc cgctggcttt gcgattatca caatcttaat gaagaagatc tgcgtaaaaa
     7561 cctctactgg catcaggata acgacgcggt tagccattta atgcgtgttg ccagcggcct
     7621 ggattcactg gttcttgggg agccgcagat cctcggtcag gttaaaaaag cgtttgccga
     7681 ttcgcaaaaa ggtcatatga aggccagcga actggaacgc atgttccaga aatctttctc
     7741 tgtagcgaaa cgcgttcgca ctgaaacaga tatcggtgcc agtgctgtgt ctgtcgcttt
     7801 tgcggcttgt acgctggcgc ggcagatctt tgaatcgctc tctacggtca cagtgttgct
     7861 ggtaggcgcg ggcgaaacca tcgagctggt agcgcgtcat ctgcgcgaac ataaagtaca
     7921 gaagatgatt atcgccaacc gcactcgcga acgtgcccaa attctggcag atgaagttgg
     7981 cgcggaagtg attgccctga gtgagatcga cgaacgtctg cgcgaagccg atatcatcat
     8041 cagttccacc gccagcccgt taccgattat cgggaaaggc atggtggagc gcgcattaaa
     8101 aagtcgtcgt aaccaaccaa tgctgttggt ggatattgcc gttccgcgcg atgttgagcc
     8161 ggaagttggc aaactggcga atgcttatct ttatagtgtg gacgatctgc aaagcatcat
     8221 ttcgcacaac ctggcgcagc gtaaagccgc agcggttgag gcggaaacta ttgtcgctca
     8281 ggaaaccagc gaatttatgg cgtggctgcg agcacaaagc gccagcgaaa ccattcgcga
     8341 gtatcgcagc caggcagagc acgttcgcga tgagttaacc gccaaagcgt tagcggccct
     8401 tgagcagggc ggcgacgcgc aagccattat gcaggatctg gcatggaaac tgactaaccg
     8461 cttgatccat gcgccaacga aatcacttca acaggccgcc cgtgacgggg ataacgaacg
     8521 cctgaatatt ctgcgcgaca gcctcgggct ggagtagcag tacatcattt tcttttttta
     8581 cagggtgcat ttacgcctat gaagccttct atcgttgcca aactggaagc cctgcatgaa
     8641 cgccatgaag aagttcaggc gttgctgggt gacgcgcaaa ctatcgccga ccaggaacgt
     8701 tttcgcgcat tatcacgcga atatgcgcag ttaagtgatg tttcgcgctg ttttaccgac
     8761 tggcaacagg ttcaggaaga tatcgaaacc gcacagatga tgctcgacga tcctgaaatg
     8821 cgtgagatgg cgcaggatga actgcgcgaa gctaaagaaa aaagcgagca actggaacag
     8881 caattacagg ttctattact accaaaagat cctgatgacg aacgtaacgc cttcctcgaa
     8941 gtccgtgccg gaaccggcgg cgacgaagca gcgctgtttg caggcgatct gttccgtatg
     9001 tacagccgtt acgccgaagc ccgccgctgg cgggtagaaa tcatgagcgc cagcgagggt
     9061 gaacatggtg gttataaaga gatcatcgcc aaaattagcg gtgatggtgt gtatggtcgt
     9121 ctgaaatttg aatccggcgg tcatcgcgtg caacgtgttc ctgctacgga atcgcagggt
     9181 cgtattcata cttctgcttg taccgttgcg gtaatgccag aactgcctga cgcagaactg
     9241 ccggacatca acccagcaga tttacgcatt gatactttcc gctcgtcagg ggcgggtggt
     9301 cagcacgtta acaccaccga ttcggcaatt cgtattactc acttgccgac cgggattgtt
     9361 gttgaatgtc aggacgaacg ttcacaacat aaaaacaaag cgaaggcgct ttcagtgctc
     9421 ggtgctcgca tccacgctgc tgaaatggca aaacgccaac aggccgaagc gtctacccgt
     9481 cgtaacctgc tggggagtgg cgatcgcagc gaccgtaacc gtacttacaa tttcccgcag
     9541 gggcgcgtta ccgatcaccg catcaacctg acgctctacc gcctggatga agtgatggaa
     9601 ggtaagctgg atatgctgat tgaaccgatt atccaggaac atcaggccga ccaactggcg
     9661 gcgttgtccg agcaggaata atggaatatc aacactggtt acgtgaagca ataagccaac
     9721 ttcaggcgag cgaaagcccg cggcgtgatg ctgaaatcct gctggagcat gttaccggca
     9781 gagggcgtac ttttattctc gcctttggtg aaacgcagct gactgacgaa caatgtcagc
     9841 aacttgatgc gctactgaca cgtcgtcgcg atggtgaacc cattgctcat ttaaccgggg
     9901 tgcgagaatt ctggtcgttg ccgttatttg tttctccagc gaccttaatt ccgcgcccgg
     9961 atacggagtg tctggtggag caggcactgg cgcggttgcc tgaacaacct tgccgtattc
    10021 tcgatctcgg gacggggacc ggggcgattg cgcttgcgct ggctagcgag cgcccggact
    10081 gcgaaattat cgctgtagat cgtatgcctg atgctgtctc cctggcacaa cgtaatgccc
    10141 agcatctggc gatcaaaaat atccacattc tgcaaagcga ctggtttagc gcgctagccg
    10201 ggcagcagtt tgcgatgatt gtcagcaatc cgccgtatat tgacgagcag gacccacatc
    10261 ttcaacaagg cgatgtccgc tttgagccgc tcactgcgct ggttgcggca gacagtggaa
    10321 tggcagacat cgtgcatatc atcgaacagt cgcgtaacgc gctggtatcc ggcggctttc
    10381 tgcttctgga acatggctgg cagcagggcg aagcggtgcg acaagcattt atcctcgcgg
    10441 ggtatcatga cgtcgaaacc tgccgtgact atggtgataa cgagcgcgta acgctcggcc
    10501 gctattatca atgacaagtt tttctacact gcttagtgtt catcttatta gtatcgcgct
    10561 ttctgttggg ctattaacct tacgtttctg gctacgttat cagaagcatc cacaggcatt
    10621 tgctcgctgg acgcgcattg tgccgccggt tgtcgatacg gtgttattgt taagcggcat
    10681 tgcgttgatg gctaaagcgc acatcctgcc attttccggg caggcacagt ggctgactga
    10741 aaagctgttt ggagttatca tttatatcgt tttgggtttt attgcactcg attatcgtcg
    10801 tatgcacagt cagcaggcgc gcattattgc cttcccgctg gcgttggtgg tgctgtacat
    10861 catcattaaa ctcgccacca caaaagtacc gttactgggg taagttatga gatcgttagc
    10921 tgatttcgaa tttaataaag cgccattgtg cgaaggcatg atcctggctt gcgaagcaat
    10981 ccgccgcgat tttccctcgc aagatgttta cgacgaactg gagcgtctcg ttagtctggc
    11041 gaaggaagaa atcagccagc ttctgccttt agaagagcag ctggaaaaac tgatcgcgct
    11101 gttttacggc gactggggat ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt
    11161 atggctggac caggtgttaa agaatcgaca gggcagtgcg gtatcattag gtgcggtttt
    11221 attatgggtc gcgaatcgtc tcgatttgcc gctgctgccg gtgattttcc ctacgcagct
    11281 gatattgcgc attgaatgtc cggatggcga aatttggctg attaatcctt ttaacggtga
    11341 atcgttaagc gaacatatgc tggacgtatg gttaaaggga aatatcagcc cgtcggcgga
    11401 actgttttat gaagaccttg atgaagctga taacattgag gtaatccgca aattgctgga
    11461 tacactcaaa gcctcgttga tggaagaaaa tcagatggag ctggcgttac gcaccagcga
    11521 agctttatta caattcaacc ctgaagatcc ctatgaaatt cgcgatcgcg ggttgattta
    11581 tgcgcaactg gattgcgaac acgttgcgtt gaacgattta agttatttcg ttgaacagtg
    11641 tccggaagac ccgatcagcg aaatgatccg tgcgcaaata aataacatcg cgcataaaca
    11701 tattgtgctg cattaattaa tcgacatttt actcaagatt aaggcgatcc tatgaaacaa
    11761 aaagtggtta gcattggcga catcaacgta gcaaatgacc tgccgttcgt actgtttggc
    11821 ggtatgaacg tgctggaatc tcgcgatctg gcgatgcgca tttgcgagca ctacgtaact
    11881 gtgacccaga aactgggtat cccttacgtg ttcaaagcct cttttgacaa agccaaccgc
    11941 tcctccatcc actcttatcg tggaccgggc ctggaagaag ggatgaaaat cttccaggag
    12001 ttgaagcaga cttttggcgt gaaaattatc accgacgttc acgaaccaag tcaggcacag
    12061 cccgttgctg atgtcgtgga tgtgattcag ttgccggcgt ttcttgctcg ccagactgac
    12121 ctggttgaag ccatggcgaa aaccggtgcg gtaattaacg tcaagaaacc acagtttgtc
    12181 agcccggggc agatgggtaa tatcgttgat aaattcaaag aaggcggcaa cgaaaaagtg
    12241 attctttgcg atcgtggtgc taacttcggc tatgacaacc tggttgtcga tatgctgggc
    12301 ttcagcatta tgaagaaagt gtctggtaac tcgccggtga ttttcgacgt gacccacgca
    12361 ctgcaatgcc gcgatccgtt tggcgcagct tccggtggtc gtcgtgctca ggtggctgag
    12421 ctggcacgag ccggtatggc ggtaggtctg gcggggctgt ttattgaagc gcatccggat
    12481 ccggaacatg cgaaatgtga tggtccatcc gcgctgccgc tggctaaact ggaaccgttc
    12541 ctcaagcaga tgaaagcgat tgatgatctg gtgaaaggtt tcgaagaact ggataccagc
    12601 aagtaatctt ttttgcttga aaaataaagt cttagcgttc tgcgttaaga cttttttcat
    12661 gggtgccaga tacaaaaaag gccgcaggct gttacccctg cggccggttt cgggcgcata
    12721 ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc caccagccga
    12781 caatcgctgc ggtaataatt
//
LOCUS       DAJTRQ010000114        12438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000114 DAJTRQ010000000
VERSION     DAJTRQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..391)
                     /gene="yafO"
                     /locus_tag="OO332_003225"
     CDS             complement(<1..391)
                     /gene="yafO"
                     /locus_tag="OO332_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5943599.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRM"
     gene            complement(394..687)
                     /gene="yafN"
                     /locus_tag="OO332_003226"
     CDS             complement(394..687)
                     /gene="yafN"
                     /locus_tag="OO332_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5943600.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(739..1794)
                     /gene="dinB"
                     /locus_tag="OO332_003227"
     CDS             complement(739..1794)
                     /gene="dinB"
                     /locus_tag="OO332_003227"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5943601.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1865..2650)
                     /gene="lafU"
                     /locus_tag="OO332_003228"
     CDS             complement(1865..2650)
                     /gene="lafU"
                     /locus_tag="OO332_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5943602.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2622..4334
                     /locus_tag="OO332_003229"
                     /pseudo
     CDS             2622..4334
                     /locus_tag="OO332_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4551..5048)
                     /locus_tag="OO332_003230"
     CDS             complement(4551..5048)
                     /locus_tag="OO332_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5943603.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5224..5745)
                     /locus_tag="OO332_003231"
                     /pseudo
     CDS             complement(5224..5745)
                     /locus_tag="OO332_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6129..6869
                     /locus_tag="OO332_003232"
     CDS             6129..6869
                     /locus_tag="OO332_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5943604.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6840..7607)
                     /locus_tag="OO332_003233"
     CDS             complement(6840..7607)
                     /locus_tag="OO332_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5943605.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7813..8391)
                     /gene="lpcA"
                     /locus_tag="OO332_003234"
     CDS             complement(7813..8391)
                     /gene="lpcA"
                     /locus_tag="OO332_003234"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5943606.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8631..11075
                     /gene="fadE"
                     /locus_tag="OO332_003235"
     CDS             8631..11075
                     /gene="fadE"
                     /locus_tag="OO332_003235"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5943607.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11118..11564)
                     /gene="ivy"
                     /locus_tag="OO332_003236"
     CDS             complement(11118..11564)
                     /gene="ivy"
                     /locus_tag="OO332_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5943608.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11745..>12438
                     /gene="yafV"
                     /locus_tag="OO332_003237"
     CDS             11745..>12438
                     /gene="yafV"
                     /locus_tag="OO332_003237"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5943609.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAE"
BASE COUNT         2930 a         3250 c         3278 g         2980 t
ORIGIN      
        1 gcatgcgaaa cgcttctgcc atttttccaa ttttatgcat ttggttgtta tctcgagcca
       61 gtttatgagg ttcaggcttc agaatggcaa tgagcaacca tgcttgctca tcaaacgcac
      121 cctgacaata taccaaatgc gcttcgtcat tcgttctgct gaattggcgc aactgtggcg
      181 gaaatggatt attcacattt gccagatgaa tatgagcaac tcgctcaaat ttgattaatg
      241 gccaggtaaa cgagtcgtcg tagagtgcat cgcgaccaaa tatatctggc aaaacaccgt
      301 cacgcttata ggaaataaaa tccgccgtta acgcatcaag ttcctctgct gtaagttgca
      361 ggcgaataag ttttgttttg aatacccgca tccttattcc ttaaagtcat tgaaatcatc
      421 atccgtcata tcattaagat attgtttagc gcggcgtgta atttcctcta atcttgcagc
      481 acttggacga aagcgcgcct ttatgggctt tttctcctct tgttcagcaa gcatgtccat
      541 taacgcttcg aatgcgctgg cacttaagag atatcctgcg gggcgattat tagaaagaac
      601 cgcaactggt tgatcaataa agtatttagc tgggttttta cgtaactcag tgatattgac
      661 cgatttttca gcgagaatcc gatgcataca gtgataccct cataataatg cacaccagaa
      721 tatacataat agtatacatc ataatcccag caccagttgt ctttccattt gcgggtcaag
      781 caacgtcaca tgcagcccca ccagacgcac accgcgcccg ccgcggcgtt catcccaggt
      841 tttacgcgcg gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgggt
      901 ggtctgctga aaatcgtcga actttaattt caccccctgg cgagcaatca gtaaatcagg
      961 ttttaccttt gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca
     1021 ttcagaccag tgatgaatat cttccgccat cgtgcgttcc acgccgacgg attttcgtaa
     1081 ccgttcgctg ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc
     1141 aaatttgcca aagcgtttaa gcagaatcac cagatcacac ttttgtacat caccgcaggt
     1201 ccgcagcccc atcgcttcca gttttgccgc cgagactttg ccgacgccgg ggattttttc
     1261 cagtggtaag gtttgtaaaa atgccggaac ttctgccggc gtaatcacaa actgaccgtt
     1321 gggtttattc atgtcggagg cgattttggc gagaaacttt actggtgcca cgcccgcaga
     1381 tgccgtcagt tgcagctcgt tgaagatcgt ctggcggatt tcctgggcga tgagggtcgc
     1441 agaaccgtgg caatggacgc tatcggtaac atcgagataa gcctcatcca gtgacaacgg
     1501 ttcaatgcgc gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta
     1561 ggcgtcaaag cgccccggaa gcaaggtgag atgtgggcat aatttgagcg ccatcccagt
     1621 aggcatagcg ctacgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc
     1681 ccgacgttcg cggctgccgc caatagcaat agggatatcg cgcagagcgg gattgtcgcg
     1741 catctccacc gctgcgaaaa agcagtccat atccacatga atgattttac gcattgctca
     1801 cctctcaaca ctggtaaagt atacagtgat ttcaggtttt gagaaatgcg taaagattca
     1861 gcatttagcg cattcgctgc gaaagcacct gctgcttatc cagcttttgc accagcggct
     1921 gcaccacttt atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg
     1981 tcagtaccat aatctcaata cgccgattgc ccgcgctttg cggattcttc gcgtccagca
     2041 gcatctggtc cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt
     2101 ccagcacccg acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat
     2161 tgtttttgta ggccatcgca tcggtatgcc cggtaataat aattttgtta tagagcgagt
     2221 cgaacactgg cgcgagctcc accagcaaag ttttaaagaa cggcataatc tgcgcgctgc
     2281 cgcgttcgaa catattgcga ttctggtcgt ctttaatcag cacgcgtaat ccctggggaa
     2341 caatctccat ctccagattc gcttccatat gcgcatcacg ggcaatggtg ttgatactgg
     2401 ttgccaactc gccaagctcg gtggcggatt tcttcttcag cagcgccgtt ttctcattca
     2461 catcacgcgc ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat
     2521 gagacgggct gattttattt aacggcgaca gcccaccacc gttaaaaatc gactgcccgt
     2581 gcagcgcggc gataatgctt tcacgctcgg atttactgac gctgttgaca atcaacttta
     2641 tcgtcgtcac caaagaggcc gagcgtattt ccgaggtttc tgcccgcttt accctggacg
     2701 cgatgcccgg caaacagatg gcgattgacg ccgatcttaa cgccggattg atcaaccagg
     2761 cgcaggcgca aacccggcgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg
     2821 acggggcatc gaagtttgta cgcggagacg ccatcgccgg gatgatgatt ctggcgatca
     2881 acctgagcgg cggcgtctgt atcggcatct tcaaatacaa cctgagcgcc gacgccgcct
     2941 tccagcaata cgtgctgatg accatcggcg acggcctggt ggcgcagatc ccttccctgc
     3001 tgctctccac cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc
     3061 atgacgtgcg ccaccagttg ctggcaagcc cgtcggtgct ctacaccgcc accgggatca
     3121 tgttcgtgct ggcggtggtg ccggggatgc cgcatctgcc gtttttgctg ttcagcgccc
     3181 tgcttggctt taccggctgg cggatgagca aacgcccgca ggcggcggag gcggaagaga
     3241 aaagcctcga aacgctgacc cgcaccatca ctgaaaccag cgaacaacag gtcagttggg
     3301 aaactattcc gctgatcgag cccatcagct taagcctcgg ctacaagctg gtggcgctgg
     3361 tggacaaagc gcagggcaac ccgctcaccc agcggattcg cggcgttcgg caggtgattt
     3421 ccgacggtaa cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgcctcaagc
     3481 ccagccagta cgctattttc atcaacggca ttaaggctga tgaagcggat attccggcgg
     3541 ataaactgat ggcgctgccc tccagcgaaa cctacggcga gattgacggc gtgctgggga
     3601 acgacccggc gtacgggata ccggtcacct ggatccagcc tgcgcagaaa gcgaaggctc
     3661 tgaatatggg gtatcaggtg atcgacagcg ccagtgtgat tgccacccat gtgaacaaga
     3721 ttgtgcgcag ctatattcct gatttgttta actatgatga catcacgcag ttgcataacc
     3781 gtttgtcgtc gatggcaccg cgcctggcgg aggatttaag cgcggcgctc aattacagcc
     3841 aattgctgaa agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca
     3901 ccatcgccac cgtgctggtc gccagtagca cggtgactaa agatcatatt ctgctggcgg
     3961 ccgatgtgcg cctggcgctg cggcgcagca ttactcatcc gttcgttcgc aagcaggagc
     4021 tgacggtgta tacgctgaat aatgagctgg aaaatctgct gaccaacgta gtgaatcagg
     4081 cgcaacaggc cggcaaagtg atgctcgaca gcgtgccggt tgacccgaat atgctcaacc
     4141 agttccagag cacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagatccgg
     4201 tgctgctggt gccgccgcag ctacgccctt tgctggcgcg ttatgcaagg ttgtttgcgc
     4261 cggggctgca tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg
     4321 gggcgttgat gtaagaatcc ggcatcggtg cacaattgcc ggatgcggcg taaacgcctt
     4381 atccggccta ccgttcaggc acaagttttt aggcatgata agacgcgcca gcgtcgcatc
     4441 aggcatctgc gcataactgc cggatgcggc gtgaacgcct tatccggcct accgttaaag
     4501 cacgatttgt aggccggata agcgtaagcg catccggcac cagcgcacag ttataaaatg
     4561 atacgctccc cggcacttaa atccgttatg tctcccgccc aatcgatggg atataaccct
     4621 cgcgcgacat cgcgatggaa cgttgagaat ggccaatcac tcacctgctt tacccagcca
     4681 tgtttctctg gatttatata aatataatca acatgatggc gataatcttt tgtattgcga
     4741 atggcatgct cccaaaaacg cggttgccag atatttttca atccgcaagc atgggtgaac
     4801 tgctttttaa tctcccgcca gcgagaggaa aaatcatcat cgctttcggg taatgtccat
     4861 atgcaatgca tatgctccgg caaaacgacc caggcgttaa tttcaaaagg tctgtctcgc
     4921 ttaacgttaa taatggcgtt acggagcgtc tggaactgga tggtcagaag atggcttcga
     4981 cgattgcgta aatttaccgt gaaaaaccat gttcccccct tgatgtaata acgacgatat
     5041 tccgacatag cgactccttt ttctcaacgg taagaggaaa aggaaaggcc gacaatgacg
     5101 ctgtgttata tcgggaaagc ggctcgcaaa aacgcacaga tttgtaggca tgataagacg
     5161 cgtcagcgtc gcatcaggca tccgtgcaca accgccggat gcggcgtaaa cgccttatcc
     5221 ggcctacaaa atcatctctt ccgtcaaaat cctgcgtgcg cctaaaaaat ggtcctgcca
     5281 gaaaggttcg gctaaccggc ttacccgaat ggtttccccg gtgcgcagtg actcaataaa
     5341 ctgaccgtcg cccagataca cccccatatg gtcggcaatc tcgcggctgt ggatatggaa
     5401 aaatagcaaa tctccccggc gcaggtcgtt attcgccaca atcgttgccc gacggtagtg
     5461 gtacatctca ttagccgtgc gcgggagttt cgcctcaagg atcttgttgt aggcataaaa
     5521 aactaaccca ctacaatcga agccttgatc gggccgcgtg ccgccccaga cgtaaggctt
     5581 gccaagctgc tgctccaggc ggtgaatcgc cacttcggtg atgttatgca ggtgatcgct
     5641 gcttaaaaag tggttgtttt ccgccagctc ctgccagcgt ctgtcactcg ccttgagcgg
     5701 cgcaggaaac cattctggat gctgttttat ctgctcccgg ttgtggtggc gctggcgagt
     5761 aaagggagca aaacgcataa cgaaaacacg cctggatgca aaaatctgcc gagcaagaac
     5821 gaagacatga aagacataaa ccggctgaat gaaggtgaga attttaaaag accggaggag
     5881 taaacgggat tattaattgc ttatttgcat attactattg agatttcata atattaggaa
     5941 ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa atttgtaaac
     6001 ccagcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag
     6061 actattccga tttgggagcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa
     6121 taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt
     6181 ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc
     6241 agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatcttt
     6301 acgtcaaaat gggcgagcaa tatcaactgc tcgacagtta taaaatctgt aaatattccg
     6361 gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg ttttatagcg
     6421 tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat attggattcc
     6481 ccaatgctta tgaccgtgcg catggttatg aagggaaata cctaatgatc cacggcgatt
     6541 gtgtatccat cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg
     6601 ttactggtgc gctggtgttt ggtcagccga gcgtgcaggt gagtatttac ccattccgca
     6661 tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc tgggagcagc
     6721 tgaagccggg ctacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggcca
     6781 atggccgtta cgtggtcagc aagccgttaa gccacgaagt ggtgcagcca caactggcat
     6841 caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat tttttgccag
     6901 gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt cggtgtggtc
     6961 tgcgagctga agtcgatttc cacatcctga tccagcaacg ttgccacgcc aaacggcgcg
     7021 cggcgggtga tccagtgtaa attagtcgag caatacgcca ttacatagcg cccgtccgaa
     7081 agcagcatgt tgaaaacgcc cttcgcccgc agttcatccg ccagtgaggc gatatattta
     7141 aatactgccg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt atgcagcagc
     7201 cagcaaaagg ctttttcgct gtcggtttca ccgaccgggc ggaagttgcc ggtttccagt
     7261 gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg
     7321 cgggtaaatg ggtgagtatt ttccagcgcc acctcgcccc gattagcctg gcgaatatga
     7381 gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta
     7441 aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc
     7501 cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag
     7561 cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac
     7621 aacagcggga gcgcatggac cccggcgaag attgatttaa gaattttcag gtcggatgcg
     7681 gcgtaaacga attattcggc ctacggttcg ggtacagatt tgtaggcatg ataagacgcg
     7741 tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg
     7801 cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac
     7861 tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggaacgc gaatttcgat
     7921 atccgccgtg ccagccattt taccgccgtc tttaccggtc agggtgatca ctttcatccc
     7981 cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat
     8041 ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt
     8101 gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg
     8161 gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc
     8221 gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc
     8281 taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc
     8341 cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcctggtaca tgaggatatc
     8401 cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt
     8461 acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat
     8521 gtgagctttg ttgtaattat attgtaaaca cattgctaaa tgtttttaca tccactacaa
     8581 ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt
     8641 tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat
     8701 ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct ggtctgtggt
     8761 cggcgtgggt actggtgcct ctgaccatta tcctcgtgcc atttaacttt gcgcctatgc
     8821 gtaagtcgat gatttccgcg ccggtatttc gcggtttccg taaggtgatg ccgccgatgt
     8881 cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc
     8941 agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg accgctgaag
     9001 agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga
     9061 ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgct
     9121 tcttcgcgat gattatcaaa aaagagtacg gcggactgga gttctcagct tatgcccagt
     9181 ctcgtgtgct gcaaaaactc tctggcgtga gcgggatcct ggcgattacc gtcggcgtgc
     9241 caaactcatt aggcccgggc gaactgttgc aacattacgg cactgacgag cagaaagatc
     9301 actatctgcc gcgtctggcg cgtggtcagg agatcccctg ctttgcactg accagcccgg
     9361 aagcgggttc cgatgcgggc gcgattccgg acaccgggat tgtctgcatg ggcgaatggc
     9421 agggccagca ggtgctgggg atgcgtctga cctggaacaa acgctacatt acgctggcac
     9481 cgattgcgac cgtgcttggg ctggcgttta aactctccga cccggaaaaa ttactcggcg
     9541 gtgcagaaga tttaggcatt acctgtgcgc tgatcccaac caccacgccg ggcgtggaaa
     9601 ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa cggaccgacg cgcggtaaag
     9661 atgtcttcgt gccgatcgat tacataatcg gcgggccgaa aatggccggg caaggctggc
     9721 ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc aactcaaccg
     9781 gcggcgtgaa atcggtagcg ctggcaacgg gcgcgtattc tcacattcgc cgtcagttca
     9841 aaatctctat cggtaagatg gaagggattg aagagccgct ggcgcgtatt gccgggaacg
     9901 cttatgtaat ggatgctgcg gcatcgctga ttacctacgg cattatgctc ggcgaaaaac
     9961 ctgccgtgct gtcggctatc gttaagtatc actgtaccca ccgcgggcag cagtcgatta
    10021 ttgatacgat ggatattacc ggcggtaaag gcattatgct cgggcaaagc aacttcctgg
    10081 cgcgtgctta ccagggcgca ccgattgcca tcaccgttga aggggctaac attctgaccc
    10141 gcagcatgat gatcttcgga caaggagcga ttcgttgcca tccgtacgtg ctggaagaga
    10201 tggaagcggc gaagaacaat gacgtcaacg cgttcgataa actgttgttc aaacatatcg
    10261 gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg cctgacgcgc ggtttaacca
    10321 gcagcacgcc aaccggcgat gccactaaac gctactatca gcacctgaac cgcctgagcg
    10381 ccaacctcgc cctgctttct gatgtctcga tggcactgct gggcggcagc ctgaaacgtc
    10441 gtgagcgtat ctcggcacgt ctgggggata ttttaagcca gctttacctc gcctctgccg
    10501 ttctgaagcg ttatgacgac gaaggccgta atgaagccga tctgccgctg gtgcactggg
    10561 gcgtacagga tgcgctgtat caggcagaac aggcgatgga cgatttactg caaaacttcc
    10621 cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt cccgaccgga cgtcattatc
    10681 tggcaccgtc tgacaagctg gatcataaag tggcgaagat tttacaagtg ccgaacgcca
    10741 ctcgttcacg tattggtcgc ggtcagtacc tgacgccaag cgagcataac ccggttggtt
    10801 tactggaaga ggcgttggtg gatgtgattg ccgccgactc gattcatcag cggatctgta
    10861 aagagctggg taaaaatctg ccatttaccc gtctggatga actggcgcac aacgcgctga
    10921 cgaaggggct gattgataaa gatgaagccg ctattctggt gaaagcggaa gaaagccgtc
    10981 tgcgcagtat taacgttgat gactttgatc cggaagagct ggcgacgaag ccggtaaagt
    11041 tgccggagaa agtgcggaaa gttgaagccg cgtaattgaa taacggagcc gaaaggctcc
    11101 gtttctttat ccgctaatta tttaaaatta aagccatccg gatggttttc caggctgccg
    11161 gtcaacgccg cgaacagcac cgttttacca tcaatcgaaa gcgcatcgtt cacattcagc
    11221 caggtgagtt tctcttgcga cgttttctca tcaatagccg agaacagccc cgtcatctga
    11281 ttagatttct cggaccacat cacagcgata cgttgcgagc cacagtcatg cggtttgcac
    11341 gcgctcatca cctgatacgt ctcatctccc aacgttacgg tttgtgcggg agtataagta
    11401 ccgcctttca tcacccatgc aggtagctta tgcccttgca ccatctgatt aaacgcagct
    11461 ttggtggttt cgccctttgc aaggctgcta atggttaaat catcctgcgc cattgcactg
    11521 gtggcgatga ccagtgcggc gactgtcgtt attgccttaa acatcattcc tcccgagctt
    11581 atcctgccca tgttattaac ctcctaaagc ctggcgataa cacgataatt tgcaaatcag
    11641 atatttccaa tatcagcatt tgttatcaat ttattctgcg ctaaggcaac tgcctgataa
    11701 ggtgttaaag ttataaaacc cttcgcataa aggagttgta caccgtgcct agtttgaaga
    11761 ttacgctttt gcagcaaccg ttggtgtggg tggatggtcc tgccaacctg cgccattttg
    11821 atcgtcaact ggaaggtatt accgggcgcg atgtgatcgt tctaccggag atgtttacca
    11881 gcggctttgc catggaagcg gcagcttcgt cgcttgcaca agatgacgta gtgaactgga
    11941 tgacagctaa ggcgcagcag tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg
    12001 agtctggttc ggttaaccgc tttttgctgg ttgagccggg cggcacggta catttttatg
    12061 ataagcgtca tctgttccgc atggcagatg agcatctgca ttataaagcg ggcaatgcgc
    12121 gagtgattgt ggaatggcgc ggctggcgta ttttgccgct ggtgtgctac gacttacgtt
    12181 ttccagtgtg gtcgcgcaat ctcaacgatt atgacctcgc cctgtacgtc gccaactggc
    12241 cagctccgcg ctctctgcac tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg
    12301 cgtatgtggc gggatgcaat cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg
    12361 acagccgggt gattaatccg caaggcgaga ttatcgctac cgccgacgcg cataaggcaa
    12421 cgcgtattga tgcggagc
//
LOCUS       DAJTRQ010000115        12226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000115 DAJTRQ010000000
VERSION     DAJTRQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO332_003238"
     CDS             complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO332_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5943610.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1033..2124
                     /gene="rutA"
                     /locus_tag="OO332_003239"
     CDS             1033..2124
                     /gene="rutA"
                     /locus_tag="OO332_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5943611.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2124..2816
                     /gene="rutB"
                     /locus_tag="OO332_003240"
     CDS             2124..2816
                     /gene="rutB"
                     /locus_tag="OO332_003240"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5943612.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DTLSPTSFARIA"
     gene            2828..3214
                     /gene="rutC"
                     /locus_tag="OO332_003241"
     CDS             2828..3214
                     /gene="rutC"
                     /locus_tag="OO332_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5943613.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3222..4022
                     /gene="rutD"
                     /locus_tag="OO332_003242"
     CDS             3222..4022
                     /gene="rutD"
                     /locus_tag="OO332_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5943614.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4032..4622
                     /locus_tag="OO332_003243"
     CDS             4032..4622
                     /locus_tag="OO332_003243"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5943615.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4633..5127
                     /gene="rutF"
                     /locus_tag="OO332_003244"
     CDS             4633..5127
                     /gene="rutF"
                     /locus_tag="OO332_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5943616.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5148..6476
                     /gene="rutG"
                     /locus_tag="OO332_003245"
     CDS             5148..6476
                     /gene="rutG"
                     /locus_tag="OO332_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5943617.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6558..6665)
                     /locus_tag="OO332_003246"
     CDS             complement(6558..6665)
                     /locus_tag="OO332_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943618.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7038..7634
                     /gene="wrbA"
                     /locus_tag="OO332_003247"
     CDS             7038..7634
                     /gene="wrbA"
                     /locus_tag="OO332_003247"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5943619.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7655..7882
                     /locus_tag="OO332_003248"
     CDS             7655..7882
                     /locus_tag="OO332_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5943620.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO332_003249"
     CDS             complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO332_003249"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5943621.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9454..10710)
                     /locus_tag="OO332_003250"
     CDS             complement(9454..10710)
                     /locus_tag="OO332_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5943622.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10971..11891
                     /gene="cbpA"
                     /locus_tag="OO332_003251"
     CDS             10971..11891
                     /gene="cbpA"
                     /locus_tag="OO332_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5943623.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11891..12196
                     /gene="cbpM"
                     /locus_tag="OO332_003252"
     CDS             11891..12196
                     /gene="cbpM"
                     /locus_tag="OO332_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5943624.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         2921 a         3102 c         3088 g         3115 t
ORIGIN      
        1 tgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
       61 cggttaaaca ggtagccgga ggatgttaca acctcctccg gcatctttaa cgtggtcgaa
      121 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
      181 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
      241 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
      301 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
      361 catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
      421 gataatcgcg tgagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcgggg
      481 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
      541 cggcaatata cagcgcctct tttgacggga agtaatacag cagattggtt tttgaaacac
      601 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
      661 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
      721 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggt aacaggatag
      781 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacggatg catgttttat
      841 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata acacaatgct
      901 aacacattaa taacattcac tttttaaaaa ctggcatccg ctttgcaaac aagccaatac
      961 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
     1021 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
     1081 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgtgcaaaaa
     1141 gcggagcatt accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
     1201 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg tctggcagcc
     1261 gtgacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
     1321 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
     1381 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
     1441 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
     1501 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
     1561 caaccgagta tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
     1621 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac acccacggct
     1681 ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc aaaccggacg cgacgttggc
     1741 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccactcg cgccaaatgg
     1801 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
     1861 aaagataccc gctccggtac tgacaccaac gttcgtcaga tggccgatcc cacttcggcg
     1921 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
     1981 gaagtcgcaa gcgtgcctgg tgcagaaggc gtgctgttga ccttcgatga ttttctgtcg
     2041 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctctct
     2101 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
     2161 tcgatccgca gcaaagtgcg cagatcgtgg tggatatgca aaacgcttat gccacgccag
     2221 gcgactactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
     2281 ttcaaaccgc tgtgaccgca gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg
     2341 gctgggatga acagtatgtc gaggctggcg gccccggctc accgaatttt cataaatcga
     2401 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
     2461 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
     2521 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttgcgc agccgtggaa
     2581 tacaccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
     2641 atggcttttt tctggagcat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
     2701 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
     2761 acgtcgaaac gttctgcgac acgctttctc ccacgtcctt tgctcgtatc gcttaaggag
     2821 tttaacgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
     2881 cgttcccggc acgctggctg atggcttggt gtatgtctcc ggtacgctgg cttttgatca
     2941 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
     3001 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
     3061 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
     3121 gggtgataaa ccggcgcgat tctacattca gtgcggactg gtaaaacctg acgcgctggt
     3181 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcac
     3241 ctccccctta tgctgatgca cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
     3301 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
     3361 agcgcggcac cggcaataat cccgacacac tggcagaaga ttacagtatc gcccagatgg
     3421 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
     3481 atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga ttatcccgcg tcggtaacta
     3541 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
     3601 ttcgcgaacg attactgtat agcggcggcg cgcaagcatg ggtggaagcg cagccgttgt
     3661 tcctctatcc cgccgactgg atggcggtcc gcgcacctcg cctggaggca gaagacgcgc
     3721 tggcactggc gcattttcag ggcaaaaata atttactacg tcgacttaac gccctcaaac
     3781 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
     3841 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
     3901 gccagaaaat ggtgatgccc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
     3961 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
     4021 aaggaattgc tatgaacgaa gccgttagcc caggtgtgct tagcaccctg ttcaccgatg
     4081 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta caggagcttt
     4141 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
     4201 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
     4261 aaaccctgac cgcgcccgtc accgctatcg tcgcttggga cagtgaattt tatgaacggt
     4321 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
     4381 ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc ctatctgatc gtcgcttgcc
     4441 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaacac gttgacgacg
     4501 ccttttttgc gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
     4561 gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
     4621 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
     4681 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
     4741 agcgccgtct gcagcgtgac cgacacgccg cccacattac tggtgtgcct gaatcgtggg
     4801 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
     4861 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
     4921 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
     4981 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
     5041 atcgaagcga ttcatcgtca caccacaccc tacgggctgg tgtggttcga tcgcagttat
     5101 cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
     5161 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
     5221 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
     5281 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
     5341 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
     5401 gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
     5461 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
     5521 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
     5581 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
     5641 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
     5701 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
     5761 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
     5821 ttactctcgt cagctatgct gcctggttcg gtctgccgca tttttccact cctggtttta
     5881 atggccaggc gatgatgttg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
     5941 gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcgag
     6001 cattcgtcgg tgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtca
     6061 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
     6121 ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc gccgaaattt ggcgcgctga
     6181 tccataccat tccggccgcg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
     6241 ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga tttaagccag aacggtaatt
     6301 tgattatggt cgcagtaacc ttcgttctgg gcgcgggtga ttttgctctc acgcttggcg
     6361 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttat
     6421 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
     6481 aaaaccgccg ggttaccggc ggtgatgact ttttgacgag cttaagaaaa aaatgcattc
     6541 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
     6601 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
     6661 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
     6721 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc gggcagaccc
     6781 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct ccaataatta
     6841 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
     6901 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
     6961 ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
     7021 acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg tacggacata
     7081 ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa agtggatggt gcagaagttg
     7141 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     7201 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     7261 gtacacctac ccgctttggc aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     7321 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     7381 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     7441 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     7501 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggctca cgccagccaa
     7561 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     7621 aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7681 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7741 cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt tgggaagaag gtagtgatga
     7801 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7861 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt ctcgcggggg agttttctgt
     7921 tatttcaccg cctcattcaa cacgctatca aacttatcca tcgggcagaa accgttggca
     7981 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     8041 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     8101 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     8161 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggctatg
     8221 ttggagtcgt gcccaactaa caccgtgatt ttcggagcac tggcgcgatc ggtgaccagg
     8281 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     8341 aacagactgt cctggtaacc gtttttcagc ttcgacaata ccttccactg ctggtcagat
     8401 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     8461 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cacctggttc ttgttgatac
     8521 ttggcgctaa aggtattttt gccatccacc agcgaacact gttgtttctc tttacaggca
     8581 ggggaatctt tatagttaac gattttttcc agtagctggt agctgtcggt aagctggagt
     8641 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8701 gtgatcatcg ggttaaaggt tgggtccata gtgcccattt tttcctggtg atgcacagga
     8761 atatcacacc cagggaatgc accagtaata aagaactggg cggtagcgac ggtacgttgc
     8821 agactattgg cataggcata aacggtgtcc ggcggcgggc attcccccga tttcactatc
     8881 ccctgctctg ccagccattc acgcatgtaa tggcccatat acacttcgag cacgccacct
     8941 ttggtggtga gttgcccacc ggggacgtcc cattcgggcc atttattcgg cgtcgactgc
     9001 tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatggcggct catcatgagc
     9061 acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga agcgagtaaa
     9121 actatccctg ccacagttgc ggcgattagc gttttgttca ttcctggcac ctcttttgtt
     9181 atcaatcatt aaagagtgtg acagaaatat gacttttttc cgggaacagg ttcgcaaaat
     9241 ggcttgttga acgcctgttt ttctatatac atcttcaaaa acaggcaagg tttgacgcaa
     9301 actatgcctg attagcccct gcctcgcgta cgccgctgat aattgcacct gtggctaaac
     9361 agaatatctg cggcgttacc aatacagaga caatatttac caataaaaaa gtgtgagact
     9421 aataacaatg gtttttagca tatgcctttg ccattaagtt gcataacgat ttaaatagtc
     9481 aagttgcgca ttcatttcct tattttttaa cttataagcc tgagaaaaaa gttcaggagg
     9541 gaggtaaaca tcataatgat tgccagtgtt tattaaataa ccatactcaa gatcttcatc
     9601 tgttgaaaag gattcatttc ccaaggagat tgatgccaca ttactattaa caataaataa
     9661 tgagcaaatg ctatctgaaa gaacagcaag aaaattcttc accgctttaa tcctatccga
     9721 cgtgtcggta tctaaagcaa gcaaattctt gttatcatta aatataaact cgggtaaatg
     9781 cagtatgcat tttttgtcat tcaagcaact atcaatgaat tgttttttaa aaaacagaaa
     9841 gctccatgac ataaaactac ccacaccctg aattttgttg tcagattgca gatcaacgcc
     9901 aaataatgtc tttacatctt ccctgagatt atagaatgcc gtatcaacaa gagcctcgat
     9961 cgtttgttta tcaacaattt taataaactc ttcgtatact tgcttttgcg tcttatggct
    10021 gcccagcgcc catgaatact catgttcttt taaaaccagt atggctctta acagacaata
    10081 tccatcgcca tctactggag cgtattttaa tccatatgtt ttattaattt cactctgaat
    10141 aaaagatatg ccagacaaat caataccagt atctaaatca gatattctcg gcatatttat
    10201 ctgttctggc tggatactca tattttcatc atctaaacaa tactcagaaa aaacatcatc
    10261 acacgcatta atgttttcga tatattccat aaagccatac caataataac ctttaaaatc
    10321 tgtgatagac tctgaacctc tgcccggcaa ggacactcta acaatatcaa catcttgaac
    10381 atattgtata gtatatgtaa tacctcctga aacccctgaa taaatttgtg tatcattagt
    10441 acgcaatgtt ctcataaata atgatagatc attatcattc tgcatttcct caaattcttt
    10501 ttctagtgat tcacattcca gacatggagt atcctcttca tttactttac gtctgagttc
    10561 attgtacaac cgcataaaac acaccagtgt gttttcaaaa agcgtgattg aatatgtttg
    10621 atttttttcg tactcatttt ttatatcgtt ccaggcctgt ttcaaataat tatttgcagt
    10681 gcaactcatt ccatgtatat tactacccat atatagcgtc tataaaattt aataaataat
    10741 gacgccctag ttaaacttaa agtgcctggt tcaactatca aaaatcgctc accctttttc
    10801 acctgtttaa aatatgttca gcaacccatc ttgatggcga cctcctctcc gcgatgattt
    10861 caataacata ttctgtgttg gcatatgaaa ttttgaggat taccctacac ttataggagt
    10921 taccttacag gggttccttc aatttgtgtt gatttacgcg agataacgct atggaattaa
    10981 aggattatta cgccatcatg ggcgtgaaac cgacggacga tctcaagaca atcaagaccg
    11041 cctatcgtcg actggcccgt aaataccatc ctgatgtcag caaagaaccg gatgccgaag
    11101 cccgcttcaa agaggtcgct gaagcctggg aagtgttaag tgatgaacaa cgtcgcgctg
    11161 agtatgatca gatgtggcaa catcgcaacg atccgcaatt taaccgtcag ttccatcatg
    11221 gcgacggtca gagttttaat gccgaagatt ttgacgatat cttctcgtca attttcggtc
    11281 agcatgcccg ccagagccgt caacgccccg ccgcacgcgg ccacgatatt gaaatcgaag
    11341 tggcggtatt cttcgaagaa acgcttactg agcataagcg taccatcagc tataacctgc
    11401 cggtttataa cgcctttggc atgatcgaac aggaaatccc gaaaacgctg aatgtgaaga
    11461 tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg acgccgggcg
    11521 aaaacggcgg tccaaatggc gatttgtggc tggtgattca tattgcgcca catccgctgt
    11581 tcgatattgt cggccaggat ctggaaattg tggtgccggt tagcccgtgg gaagcggcgc
    11641 tgggtactaa agtcaccgtt ccaacactga aagaaagcat tttgctgact atcccgccag
    11701 gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc aaaaaacaga
    11761 ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat gaaaacactg
    11821 ccgcgctgtg gcagcaactg gcagacgccc agtcgtcttt tgatccacgt aaagattggg
    11881 ggaaagcata atggctaatg ttacggtgac ttttactatt accgaatttt gcctgcatac
    11941 cggcatctct gaagaggagt tgaatgaaat tgtcggtttg ggggtggttg aaccgcgtga
    12001 gatccaggaa acaacctggg tatttgacga ccatgccgcc attgtggtgc aacgcgcggt
    12061 acgcctgcgt catgaactgg ctctggactg gccggggatc gcggtggcgc tgacgttaat
    12121 ggatgatatt gcgcacctga agcaggaaaa ccgcctgcta cgccagcggc tttcccggtt
    12181 tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac gcatcc
//
LOCUS       DAJTRQ010000116        12102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000116 DAJTRQ010000000
VERSION     DAJTRQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(124..291)
                     /locus_tag="OO332_003253"
     CDS             complement(124..291)
                     /locus_tag="OO332_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943625.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(479..1318)
                     /locus_tag="OO332_003254"
     CDS             complement(479..1318)
                     /locus_tag="OO332_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5943626.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1320..2006)
                     /locus_tag="OO332_003255"
     CDS             complement(1320..2006)
                     /locus_tag="OO332_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5943627.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1993..2307)
                     /locus_tag="OO332_003256"
     CDS             complement(1993..2307)
                     /locus_tag="OO332_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943628.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2304..2789)
                     /locus_tag="OO332_003257"
     CDS             complement(2304..2789)
                     /locus_tag="OO332_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943629.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2786..2950)
                     /locus_tag="OO332_003258"
     CDS             complement(2786..2950)
                     /locus_tag="OO332_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR5943630.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2961..3254)
                     /locus_tag="OO332_003259"
     CDS             complement(2961..3254)
                     /locus_tag="OO332_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR5943631.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="OO332_003260"
     CDS             complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="OO332_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5943632.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3775..4482)
                     /locus_tag="OO332_003261"
     CDS             complement(3775..4482)
                     /locus_tag="OO332_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5943633.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4737..4889)
                     /gene="kil"
                     /locus_tag="OO332_003262"
     CDS             complement(4737..4889)
                     /gene="kil"
                     /locus_tag="OO332_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR5943634.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4874..5005)
                     /locus_tag="OO332_003263"
     CDS             complement(4874..5005)
                     /locus_tag="OO332_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR5943635.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5030..5998)
                     /locus_tag="OO332_003264"
     CDS             complement(5030..5998)
                     /locus_tag="OO332_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR5943636.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6166..6408)
                     /locus_tag="OO332_003265"
     CDS             complement(6166..6408)
                     /locus_tag="OO332_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943637.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6611..6943)
                     /locus_tag="OO332_003266"
     CDS             complement(6611..6943)
                     /locus_tag="OO332_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5943638.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7404..7808)
                     /locus_tag="OO332_003267"
     CDS             complement(7404..7808)
                     /locus_tag="OO332_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943639.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7805..8437)
                     /locus_tag="OO332_003268"
     CDS             complement(7805..8437)
                     /locus_tag="OO332_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5943640.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8541..8756
                     /locus_tag="OO332_003269"
     CDS             8541..8756
                     /locus_tag="OO332_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5943641.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8876..9169
                     /locus_tag="OO332_003270"
     CDS             8876..9169
                     /locus_tag="OO332_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCR5943642.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9202..9348
                     /locus_tag="OO332_003271"
     CDS             9202..9348
                     /locus_tag="OO332_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCR5943643.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9341..10174
                     /locus_tag="OO332_003272"
     CDS             9341..10174
                     /locus_tag="OO332_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCR5943644.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10164..11600
                     /locus_tag="OO332_003273"
     CDS             10164..11600
                     /locus_tag="OO332_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5943645.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11677..>12102
                     /locus_tag="OO332_003274"
     CDS             11677..>12102
                     /locus_tag="OO332_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5943646.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWG"
BASE COUNT         2923 a         2971 c         2695 g         3513 t
ORIGIN      
        1 ttttcagtcc acatgcgatt gaggcaagag ttgcgctact gatgccaaca tcgccagtcg
       61 attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccaa taaaaaaccg
      121 ccatcaggcg gcatggtgtt ctttcagttc ctcaattcga atattggtta cgtctgcatg
      181 tgctatctgc gcccatatca tccagtggtt atagcagtcg ttgatgtcct ctgcttcgat
      241 aaccctgtcg aatggctctc cattccattc acctgtgact cggaagtgca tttatcatct
      301 ccataaaaca aaacccgccg tagcgagttc agataaaaga aatccccgcg agtgcgagga
      361 ttgttagttg ctatctgctg cttccttggt cattaccata tccacccaat ttcaccagac
      421 atgattctcg caatcactat tatcaccaaa gtgataatca caactttaac tggcggcatc
      481 attcaccatc ctgctccggc ggttctagca gcggcatcca gtgggttacg tcatccaaga
      541 tatttcctga taaatatgtg aaagctctat attttttgta atcaattgga ctcattaccc
      601 agttccaata tgctgccact atttgacctt gactaaatgc cagtaacatt ttggtgtctt
      661 ccggcattcg ctcactacag cttatccaac catccggagt taccggatag ttgcgcattg
      721 cgacctttaa tgcctcatag aagcaacctt tcagattgtt gaactgacgc ccatttagcg
      781 ggccatgttc agtcagcatg ttgtgtaatt tccatgccgc gtcgtttact tcgttggatg
      841 acaggggagg caacttgtaa gtttggctta caggttcggc accatgaagc atggcgtcgc
      901 tccgctctat gccatccagc gcgattcgta gtgcctgaat tgtggtagag ctatcgtttg
      961 gggctattcc atatcgctcg aatacagcta aatggttgcg cataatctca ggcgtaagct
     1021 ctttgtaagc ataagcgaga ggctctgatg cattatccgg cacaaccgac gcaggcgcgg
     1081 cagcataaac aggaataacg tccgcttgct ctttattgct ttcatccgtt aaagcccaga
     1141 ataatttccc ggccggatgt ttgaaaatat aagcaactgg ctctgcttcc agcgatgcca
     1201 gtgcaattcg tgccagttct tccgcttctt ctgctggcag tacaacgttg ctaccaggtc
     1261 cgtatgtttc gcgccactgc ttgattgtca gtagtcgctc tttggtaata gtggtcatct
     1321 cactctcctt tgatgcgaat accagtagcg cggattgcat cgatgacttc agaaactttg
     1381 tatgccatta ccgtttggta atcctcgtga aaatctgttc gatgaagcat gctgctacgt
     1441 tccgggagca gtatttcccg ctcctccagt tctgctatgc gcttctctgc ggattccagc
     1501 ttctcgcgca tatcgtcaac gtactcgacc agagaaccgc cagcaggaat ttcgcactcc
     1561 tcgaccagtt ggaagtagat atcagctgcg gcccgtgtgt tgctatacct agcgtcgccc
     1621 atctcacctt cacgaagagc atcgcgttcg gcggtaagat tggctatttt gctgtctttg
     1681 ccttccagct caacgcgcag cttccctacc gtaagcgcaa tatcctcgtt ctcctggtcg
     1741 cggcgtttga tgtattgctg gttcctttcc cgttcatcca gtagtgcctg cgctacttca
     1801 gggttgaaag ctgcgatata acgagcgttg ttctctgcgt tgttctgtcc atcaaagccg
     1861 gtccatttga taacgtcttc acatcgttta tcaccaggtg tatgcaccgc atatgtacca
     1921 gtaccagacg aaataaatgc gacccattca ccctgtgttg ccagttgcgc cgctttacgc
     1981 agtgcctggt aattaatttc gctcactggt tgcctccttt gctgggcttt ctaacttttg
     2041 agtggttgta tcaaattcaa acaacttaac cacgtcatca aacaggacat aatcaccatc
     2101 agaatcttca gtcatgtcag cgccacaatc ctgaccgcat gagtcacaac cacccatatc
     2161 aagctcgtat cgcttcaggt ttgcgatatt tgataaattc agcgccagta cagccaggtc
     2221 ataaacctct tcggcagtga catcgctgct cagtcccatt tcatggcgat atatgatttt
     2281 ttctactcgt tgttttgtga ttgtcatttt tgctcgcctc cctgttcttc cagaaaaata
     2341 cgcatagtct caagcatctc ttcggtgtca tacggtgaca acttgtcacg caggatgtgt
     2401 tcaatgctgt taatgaactt gcggattgct ttgcgttcaa tttcagccag aaaagcatcg
     2461 gttgccgggg tttcaggttt aatggcttca agcacggcgc gaatgacatc ggtatcgttc
     2521 tccacccatg accattcgct tgtatcattc cagtcgtggt ctatgatagc tgtttcattg
     2581 aatgcctcga ctgccgtatc cgggattact tcatgattta aagctgcctt cagccccgca
     2641 ttctccgccg ccagcgccgc gcacttggcc tccgcttcag caaatttacg caccagatat
     2701 tcagcgtttg tttcgttaac ctttaaatca cttgggatgc atttaccttt cagaaatcca
     2761 tccatctcaa ttagtgtcat ttgtttcatt tcttcccatt ccgccacatt gcattcagat
     2821 atttgttttg atttactgac ggaaaagaat ttcttttaag caattcctct ctcgatggca
     2881 ttggctttac gcgttggcga ataatcattt ctgccggaag aatgccgaga ttgtatgcaa
     2941 gtcctctcat ggtaaattcc tcagtcatta ctgatagcgc catagcgtga gcggtaatta
     3001 cgcaggcgcg ggtcaatttc agggaagtgg gtatatgtgg ctttgcggaa tggtcggatt
     3061 gatgtctggt aaattcgctc gcgctcttct ttctctgcaa gccatatgca gtggcgaaat
     3121 tccttttcct ctttcgtttc ctgcggtagc gacattatcc ggtcgtagtt tttcctgaat
     3181 ttatccagca cctccgatac ggaattgccg gaacagcggc gcgggtcatc cgcaccatac
     3241 tgaggcgctg gcatgatttt ctcctgatta aattgcgtga atagcgtgac gagggaaggg
     3301 gagagttact ggtgcaaagg gtatatcgtc gtcaaaatcc atcggaggtt cgttgtgttg
     3361 tgctggtgat gattgctgct gtggcttctg tgattgcctg ctggctgctt gttgtttgct
     3421 gtcgccaatg ccgccaagca tttgcatcac gccattaatt ccgacatgaa cctcggttgt
     3481 gtaacggtct tgccctgact ggtctttcca ctttctggtt ctcagcattc cctcgaaata
     3541 aatctgatca ccttttttca catactgccc cacgacctca gccagtttcc cggatacagc
     3601 aacacgatgc cattcagtca attccttttg ctcgccagta tttttatctc gccattgttc
     3661 tgacgtggct attgtcaggt tagcgaacgc tgttcctgat ggtgagtatc gaacttccgg
     3721 gtcttgtcct acccgaccaa ggataatcac cttatttacg cctctgcttg ccatttatgc
     3781 cgcctgtttt agttcgttaa ctctgatgtt cattacctga acgcatttag cctgcgcttc
     3841 ctcgttgcca gccattaatt gccagtcacg ctgataacgc tcgatgagtt ttttcttgtc
     3901 agtttctgtt gacgcataat cgctgaagtc tttcaggatt tgttcgcagt caaccgatgg
     3961 agatttctgg ttggtatttt ctggtgatgg tttgttatct gatgctggga ttgcccatcc
     4021 cggcagcgat ggagggagcc agtaaaatcc tgttccatcc ttcagttttg ccctgtgcca
     4081 cccatgcttt ttatcgagag atgtttgtgc gaaaccttcc tcaaggttat acagataccg
     4141 accgattccc cactgaacgg cagcacgctt cattgcacct gaacgaccac ctttgacggc
     4201 ttctacctgc gtgttttcag cagcatccca tttggttacc cattcggaat caatcctgat
     4261 tgatatgccg cattcaacgc ctccgttgtt gggaatatcg cggtattcat tgcgccatcc
     4321 tgctttgccg caaacatcgt ccaggcgttt catgattgcc cggttcgtga cataagccag
     4381 caccatagcc cacactttgc catcgcgtgt tttaccgctt tgctgtattc gccattcgat
     4441 atcttcaggg ctgaatggct catcgaattt attcaaatcc ataaatcacc tcagaatgga
     4501 catggcccaa gaaaataacg ctgatttaat acttcgactc gggacaaatt aaggcatacc
     4561 cgcattcctt cgcggtcgcc attatggcga taccagagag ctttctgggt gtacatgcgt
     4621 ctctgtaact tgctctcctt cactgtggtt gcaagtgaca tgaatatctc cttcgtttcc
     4681 gattaattct ttcatctgac gaatgaattc ttcgtctgac cagttatctg taaaactcat
     4741 ttcctgcgat accacggaag gttgatagct gacttcatcg ctttatttgc ttcaagccac
     4801 attttggaat caccaataaa tctggctatt actgctttgt tctgtgcagc acgaagcatc
     4861 tggtgattga tggctatttc attgcgcata ataagacatc aactcttttc catccgtcac
     4921 gtaatttacg ggtgattcgt tcaagtaaag attcatttaa ttggaaggca cccatgcgag
     4981 cgcctcccgc gattgcgtaa atcatgggtg gttccttatg ttggttttat tagtaggtta
     5041 tttttgttgc gaatacttcg ccttttacga tggctgttat gatattttta gcaacatctt
     5101 ctgatgcgcc aaccttgata aggtcagcaa gtattttgtt atttacttct tttcggtgag
     5161 ctttatcctt tgctctacgc tcttcttcgt ccttgattct tttttcttct gctattctgg
     5221 cttgctcttt tgcttcagct tcgcgccgga ttcgttcagc ctcctcctgt gcttttcggc
     5281 gttctgcttc aattgccgcc tgcttttctc tttcagctcg ttctgctgcc tcttttgctt
     5341 cgcgctgtgc tcgttgctcg gcttcaatgc gttcacgctt tgcacgttcc gctgcggcct
     5401 tagcttctgc ttctcgcctt gctgctgctt caatttcggc ttttgccttt gcttcggctt
     5461 ctgctctggc tttctcttca gcttctcttt ttaagcgttc ttcatgctct cgcttttcct
     5521 gctccgcttt gagtcttgcc tcttctcttt ggcggtcaaa ttcgcgatcc atcaaaatcg
     5581 ctatttcatg gtcagactca atttgctttg cgagagcttc agctgctgcc ttagcttctt
     5641 cttcggcttt aatccgcgcc tgttcctcct cataatcagt aagaggctgg cgcgccttgg
     5701 ctttcagctc atcaagtcga tcatgcactg ttttgcggtt ggcatcaatt agctttggaa
     5761 tttccttcag ttcagcaaca aggtctttgc caagaccatc gagatatgtt ttcgtctgcg
     5821 caactttata cgccagagaa gcgatctcct ttctgccctt tgccgttgtg atatcaggca
     5881 caaaggacat aacttcacgt tcaacctttt gaaggatttc ttcaatctgg tcggcagact
     5941 gaaatacagt cattgcattt gctttttcaa taacaactaa atctgttact tcactcatat
     6001 ttcccccgtc aaaaaaattg ccctcacact ggagggcaaa gaagatttcc aataatcaga
     6061 acaagtcggc tcctgtttag ttacgagcga cattgctccg tgtattcact cgttggaatg
     6121 aatacacagt gcagtgttta ttctgttgct tattccaaag caaatttaac catcaacaaa
     6181 ctcttctggt aatttatcaa ccagttgatg acttattatc agccatttgc catccttcgt
     6241 ttcgtatgca tatttctggt cttttatcat catgtgttcg gctactgccc tcactgcctg
     6301 atcggtgaca tcttctctct ttcctaccca cattcctttt tcagtgttta atgttccttg
     6361 aaaaatacga ccacttaata ggcttgcgcc catagttttt actctcatgt atcagtcctc
     6421 aaataagtgg tttgctgcga aagtaaatac gcttaagtta cctgttattt atcccaccaa
     6481 gttccgtatc tatctatcca gttacaccaa tcatcgacac tctattttgt tgtgtcgcat
     6541 tttggcaact ggcaagaata tctaccttct ttgtaaagtc ggcgtttgac tttcttgagc
     6601 atggctcacc tcaatcgtaa taagctggaa ttgattttcc gcgttgcttc tggcggcctg
     6661 agcaagtcac acctatttca ctgcgtggct tgctgtacca tgtgcgctta ttcttgcgct
     6721 cagtacgctg caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta
     6781 tcggattcag tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc
     6841 ggttaactgg ttttgcgctt accccaacta acaggggatt tgctgctttc cattgagcct
     6901 gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc catctggatt ctcctgtcag
     6961 ttagctttgg tggtgtggtg gctggtagtc tagctccagc ttgttgagtc tcattcggag
     7021 gggtataacc ggcaccccag cgatttttcc atgcgacaac gtgcgcgtta tggcggcctt
     7081 atcgcccgcg gctccccatc tcgtccacgc tattgctagc gttgggagcg cttcaccgct
     7141 caacagtagg taagcacttg ccagtgacta gctggcttca ccacacccca aagccttctg
     7201 ctttgaatgc tgcccttctt cagggcttat tttttaagag cctcaccttc atggtggtca
     7261 gtgcgtcctg ctgatggctt aaaattacaa gaaagattgt atgttgtaaa caagaaatat
     7321 tgtgaaaagg ggcgtgaaaa acaaactcca ttgtttttaa atgaaaaata gtttgttttt
     7381 ggttatcgag attgaggtgg ggattactgg ttgcaggttc cgactacatc accaacaaat
     7441 gatttggttg atgtaagttg ttgcatacct gggatattca ttactttgga gtaaagagct
     7501 tttttgtctg tagtgattga ccaagtttca acggttattc ctcctccaga ctggtattct
     7561 cctaccatag tgttcgatga caaagcagtg tatttcatct ctggatagac gccagaaact
     7621 gattcataaa ctgatgattt atcgccattt attgttacgt ggaaaacgga atcttccgtg
     7681 ctgtcttttg taaactcgta acgatcgcca ttcattgacc cgtacccgtg caggtttgtg
     7741 acaatccagc attcagaatt ggcgctggta gttaagagta ttgagagtag cgccgcaatc
     7801 ctgatcatac gaattttacc ctcgcttcca cgacaacacc gataatcttg cagttcccgt
     7861 tgataggagt cataggccat gaagggttca ggcctttcag gtacttctgc ccgccatcta
     7921 taaccagttt cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc
     7981 cattcactgg ctcgcgtcca gtatctacta acaccatatg accttcaggg atgctttgac
     8041 ctacaggtga ggtcatggaa tcaccttcaa ccttcagcca gaatccatcg cctaataagt
     8101 taacgtcact gtcataccat tcatcaatgt ccttgatatc gtagggttca caagcttcac
     8161 accacgaacc agctctaacc atgctaatca atggatattt ccctttgggt tcaacatgcc
     8221 caacaaatct aacattcgaa tcagaggtgc cattgagcag ccagtcaaca cttacgccaa
     8281 gagctgacgc aagttctggt aaaaagcgtg gtcgcttagt tttaccgttt tcgagctgct
     8341 ctatagactg ctgggtagtc cccacctttt gagcaagttc agcctggtta agtccaagct
     8401 gaattctttt gctttttacc ctggaagaaa tactcataag ccacctctgt tatttacctc
     8461 caatcttcac aagaaaaact gtattttaca aacaagatac attgtatgaa aatacaagaa
     8521 agtttgttga tggaggcgat atgcaaactc tttctgaacg cctcaagaag aggcgaattg
     8581 cgttaaaaat gacgcaaacc gaactggcaa ccaaagccgg tgttaaacag caatcaattc
     8641 aactgattga agctggagta accaagcgac cgcgcttctt gtttgagatt gctatggcgc
     8701 ttaactgtga tccggtttgg ttacagtacg gaactaaacg cggaaaagcc gcttaagaca
     8761 ttcccgctct tacacatccc agtcctgaaa aagggcatca aattaaacca cacctatggt
     8821 gtatgcattt atttgcatac cttcaatcaa ttgttatcta aggaaatact tacatatggt
     8881 tcgtgcaaac aaacgcaacg aggctctacg aatcgagagt gcgttgctta acaaaatcgc
     8941 aatgcttgga actgagaaga cagcggaagc tgtgggagtt gataagtcgc agatcagcag
     9001 gtggaagagg gactggattc caaagttctc aatgctgctt gctgttcttg aatggggtgt
     9061 cgttgacgac gacatggctc gattggcacg acaagttgct tcgattctca ccaataaaaa
     9121 acgcccggct gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     9181 gatcaatcca caggagtaat tatgacaaaa caactcagtc cttaccagga caaaattcac
     9241 aaacacatac tacgtgatcg cttcctgtcc agcttcaagc agcctggtcg attccgggct
     9301 gagttggaaa aagtgaagct gatgcagaag gagaaaggtc atgagtaaca tatctaatct
     9361 agccgaagcc agagaggcca gaaggctaca acaaccgcat caaagcagcg gtaaggggta
     9421 tgccttgctg caccgtaaaa ttatggatgt gccgttttac aaggacgcag aagcagcgca
     9481 tctgtgggtt cacttaatcc tcaaagcaaa gcatacgcct gagtatgtaa tgactgacgc
     9541 aggagaaatt ctggtaggca gagggaagct acttggcggt agaaactctc tggcgtttga
     9601 aacaggactc aaaccagatc gcgttcagta cctgcttaga aagttcaaaa aactcggcat
     9661 gattgactgg gtttcacacg gtaaattctc agttttctcg gtagagaaat atgacgatta
     9721 tcagtcaaat tttgtaccag cagattacca gcaaattacc acagataaag aatataataa
     9781 tattatctct aatactgacg tattagagag taccgcagca gacaaaaagt ctgacaagaa
     9841 aaaaccttcc gttagctgtc aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc
     9901 gccaaaaatc cgcgcactga atgacaagcg taaaaaccag atccgaacgt tctggcgcaa
     9961 agccggagtg ataacccgcc agcttgacgg gcatgggttc acgatgcagg actggagaaa
    10021 ttatttgagc tacgtaggcg aaaattgccg atggatgttc gaagagcgcc caaaccatca
    10081 gcgcggaacc gtctggcaca aaaagggatt tgatttcctg cttaacgata atacctacct
    10141 gaaagttcgt gagggtgaac acgatgaccg ataattttta tgcgccgccc catagcatcg
    10201 aggcagagca ggcggtgatt ggtggattgc ttctggatga tgacagcagt gagcgcgtcc
    10261 agaaggttct ggcgatgctg aagcctgatt cattttacag ccgaccacac aaaatcattt
    10321 tcgaagaaat aaccagaatg caccgggagc aaaagccagt agatggcctg acgcttttcg
    10381 atgaactgga gcgtaaatcg ttaacggcgt ctgttggcgg ttttgcttat atcgctgaga
    10441 tcgcaaagaa cacgccaagc gccgcaaaca tcgttgccta tgcaatgcag gttcgcgaaa
    10501 ccgcaatgga acgctacgcc atcaaccgca tgactgaagc gacggaattg ctctattccc
    10561 gcaacggaat gactgcgacg cagaagtacg aagctattca ggcgattttc acgcaactga
    10621 cagaccatgc aaaaacagga tcgcgtcgcg gccttcgctc atttggtgag gtcatggaag
    10681 actgggttag cgaccttgag aagcgatttg acccatcagg cgaacaacgg ggaatgagca
    10741 cagggatccc atcgctggac aggatgctgt caccgaaagg tctggtgaaa ggctctctgt
    10801 ttgtcattgg cgctcgacct aagatgggga aaacgacgct atacagccag atggcaatca
    10861 actgcgcagt gcatgagaaa aagcccgctc ttatgttcag ccttgaaatg ccaggtgacc
    10921 agatactgga aaaactggta gggcagaagt ctggtgttaa cccgaatatt ttttaccttc
    10981 cggcgacaaa tgacgctgat gacggctatc agggtgatta cgatggtgac ttcaacaggg
    11041 cgatcgaaac agccaatcgc ttgagtgaaa tcgacctgct ttacatcgac gacacgccgg
    11101 gattatctct ggctcaaatc gtcagcgaaa gccgtcgaat caagcgagaa aaaggatgtg
    11161 ttggcatgat tctggtcgat tacctgacac taatgaccgc tgaaaaggcc gatcgcaacg
    11221 accttgctta cggcatgatc accaaaggac tgaagaacct tgccaaagag cttgattgcg
    11281 ttgttgtgct tctgacacag cttaaccgcg cactggaaag ccgaaccaat aaacgcccat
    11341 taccaagtga ctcacgagat acagggcaga ttgaacagga ttgcgattat tgggtgggga
    11401 tccatcgtga aggtgctttt gatgacagtg ttccacctag tgaaaccgaa ctaatccttc
    11461 gtctcaatcg tcatggcaat accggcacgg tgtattgcat tcaggcaaat ggcgctattt
    11521 atgacacaga ccaacagtct gctgaaatgc gccgacgtga acgcgaggaa ccgcagtcca
    11581 agaagaaagg aggattctga tgaccatcta catcactgag ctaataacag gtctgctggt
    11641 aatcgcaggc ctttttattt gggggagagg gaagtcatga aaaagctaac ctttgaaatt
    11701 cgatctccag cacatcagca aaacgctatt cacgcagtac agcaaatcct tccagaccca
    11761 accaaaccaa tcgtagtaac cattcaggaa cgcaaccgca gcttagacca gaatcgaaag
    11821 ctttgggctt gccttggtga cgtctctcgt caggttgaat ggcatggtcg ctggctggat
    11881 gcagaaagct ggaagtgtgt gtttaccgca gccttaaagc agcaggacgt tgttcctaac
    11941 cttgccggga atggctttgt ggtaataggc cagtcaacca gcaggatgcg agtaagcgaa
    12001 tttgcggagc tattagagct tatacaggca ttcggtacag aacgtggcgt taagtggtca
    12061 gacgaagcgc gactggctct ggagtggaaa gcaagatggg ga
//
LOCUS       DAJTRQ010000117        11839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000117 DAJTRQ010000000
VERSION     DAJTRQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(35..1399)
                     /gene="tdcG"
                     /locus_tag="OO332_003275"
     CDS             complement(35..1399)
                     /gene="tdcG"
                     /locus_tag="OO332_003275"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5943647.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1471..1860)
                     /locus_tag="OO332_003276"
     CDS             complement(1471..1860)
                     /locus_tag="OO332_003276"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5943648.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1874..4167)
                     /gene="tdcE"
                     /locus_tag="OO332_003277"
                     /pseudo
     CDS             complement(1874..4167)
                     /gene="tdcE"
                     /locus_tag="OO332_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(4201..5409)
                     /gene="tdcD"
                     /locus_tag="OO332_003278"
     CDS             complement(4201..5409)
                     /gene="tdcD"
                     /locus_tag="OO332_003278"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5943649.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5435..6766)
                     /gene="tdcC"
                     /locus_tag="OO332_003279"
     CDS             complement(5435..6766)
                     /gene="tdcC"
                     /locus_tag="OO332_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5943650.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6788..7777)
                     /gene="tdcB"
                     /locus_tag="OO332_003280"
     CDS             complement(6788..7777)
                     /gene="tdcB"
                     /locus_tag="OO332_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5943651.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7876..8814)
                     /gene="tdcA"
                     /locus_tag="OO332_003281"
     CDS             complement(7876..8814)
                     /gene="tdcA"
                     /locus_tag="OO332_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5943652.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9004..9348
                     /gene="tdcR"
                     /locus_tag="OO332_003282"
     CDS             9004..9348
                     /gene="tdcR"
                     /locus_tag="OO332_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5943653.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9604..10143
                     /locus_tag="OO332_003283"
     CDS             9604..10143
                     /locus_tag="OO332_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943654.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10165..11341
                     /locus_tag="OO332_003284"
                     /pseudo
     CDS             10165..11341
                     /locus_tag="OO332_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         3338 a         2874 c         2570 g         3057 t
ORIGIN      
        1 tttgtgcacc caaggatgaa agctgacagc aatgtcagcc gcagaccact ttaatggcca
       61 gtcctccgcg tgatgtttcg cggtatttat cgttcatatc tttgccggtt tcatacatcg
      121 tctcgatcac tttatcgagt gaaacacgcg gtgccgaggt gcggcgcatc gccatccgcg
      181 cggcgtttac tgctttcacg gcattaatgg cattacgttc aatgcacggg atttgtacct
      241 gtccggcaac cggatcgcag gtcagcccaa ggttatgctc catcgcgatt tccgccgcat
      301 tgcatacctg cgccggactg ccgcccagta gttcagttaa ccctgccgct gccattgaac
      361 aggccacgcc aatctccccc tgacagccga cctccgcgcc agagatggag gcgttcattt
      421 tatacagcgc gccaatagcc cccgctgcca gaaaatagcg ggcaattgac cgctcgttta
      481 ccggacgacg gaacttatcg taataagcca gcactgccgg aataatgccg cacgcaccgt
      541 tagtcggtgc cgttaccacg cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt
      601 acatgttgat ccagtcgatg acattcatcg gatcgttaga gatgttatcg ctagaaacca
      661 gctgacgatg cagcgctacg gcacgacgcg gcacattgag cggaccaggc agcacgcctt
      721 cagtgttcat cccgcgttca ataccgtcat gcatcacttg ccagatacgg gcaaaccctg
      781 cgtcaatttc cgctttgctg cgcagcgcca gctcgttgtg catcatcagg ccagatatag
      841 acaggccgtt gtaatcacac attttcagca gttcacctgc tgagtggaaa tcgtaaggta
      901 cggacgtttc gacatcgtgc gacaggccga agtgttcttc ttcgacaata aacccgccgc
      961 cgacagagta ataggtttta cttaatagcg cttcctgtcc cttccaggca gtgatccgca
     1021 ttccgttctc atggcgaggc aacatttcgg gatggaagat aatgttcttt gctacaggaa
     1081 aatcaacaat atgcgcacca gatgccactg gcagccgccc gctgcgcgtt actaactcta
     1141 taaatgcagg gatctcatca atgacaacat cctgcggact gtttcctgcc agtcccatga
     1201 taatggcgac atccgtggca tggcctttgc ccgtcagtga caacgacccg tacagatcga
     1261 ccacaatatg gctcgtcgcg gttaataagc cgctactttc cagccgatca ataaaacttt
     1321 ttccggcatt cattggcccc acggtatgcg aactggaggg accaatccca attttgaaaa
     1381 tatcgaatgc actaatcata tccacaccct cggattgccg ttcagtgaag tggagcggaa
     1441 cgaccttgcg accgtcccgc tcacgaggct ttacgcacta cgtactgcga tagcttcaat
     1501 ttccagcttc acatctttcg gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt
     1561 cgcctgatgc tcatcgaaga actgcttata gacttcattg atggtggcaa aatcattcag
     1621 atcggtgatg aacacggtca tcttgatgat atcgcccacg ctcagcccgg cagcaaccac
     1681 gatcgctttg acgttttcga ggcttaaacg cgcctgatct tgcacatcag ccgggatctc
     1741 accggtctgc gggcaaaccg gaatttgccc ggaggtgaag accatgctgc ctaaatcaac
     1801 gccctgaaca taagggccga ttgcgcctag ggcacgttgc gtttcgataa tctttttcat
     1861 acatcctccg gcgtcagagc gcctgggtaa aagtacgtga aataacatcc tgttgctgtt
     1921 cacgggtcag tgcgttgaag cgcacggcgt agccagagac acggattgtc aggttaggat
     1981 atttttccgg gtgctcgatg gcatccagca gcatttcccg attcattacg ttgacgttga
     2041 ggtgttgacc gccttcgaca tccgcttcgt ggtggaaata cccatccagc aggccgacaa
     2101 ggttggtttt acgtactgga tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt
     2161 acgagatccc atctttggcg taggtgaacg gcagtttcgc caccgacgtc aatgaggcca
     2221 cggcaccttt gcggtcacga ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg
     2281 cgcgacgacc gtccggcgta ttaccggttt tctggccgta caccacgtta gaagtgatag
     2341 tcagaatcga ctgggtaggg acggcgttgc gataggttgg cagcgcttta attttcttca
     2401 taaagcgttc aaccaggtcg caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact
     2461 gcggatattc accgtcgatt tcaaagtcca ccgccaggcc gttttcgtca cggattggtt
     2521 tcacgcgggc atatttgatg gcagacaggg agtccgtcgc caccgacagg cccgcgatgc
     2581 cgcatgccat agtgcgatag acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc
     2641 tgtacttgtc gtgcatgtag tgaatgatat tcagcgcgct gatgtactgc accgccagcc
     2701 agtccatgaa gtgatcgagg ctgtccatca ctttgtcgta atccagcacg tcgtccatca
     2761 gcggtgctgt tttcggcccg acctgaatct tcagcttctc gtccaccccg ccgttaattg
     2821 cgtagagcag cgttttcgcc aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa
     2881 tcaccattgg gctgacgcag caggcaatcg cgtaatcgtc gctgttgaag tcagtacgca
     2941 tcagatcgtc attttcatac tgcaaggaag aggtgacgat cgacacctgc gcggcatatt
     3001 ttttgaaggc aatcggtaat tcttccgacc aaagaatggt caggttaggt tccggtgccg
     3061 gccccatagt gtgcaggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc
     3121 gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga
     3181 atcaaattcc ggtgtacgca ggaagcgtac catacggatc ttcatgatga agtgatcgat
     3241 cagttcctgt gcctgctgct cattgaggac gccagcttta aagtcgcgct caatgtagat
     3301 atccaggaac gatgccgtgc gccccagcga catcgcgccg ccattttgcg atttcactgc
     3361 cgccagataa gcgaagtaga gccactgcac cgcttcctgt gcattctgcg ccgggcgaga
     3421 gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc
     3481 tgccagctcc tcacgcagac ggatggtggc ttccagatcc tcgccttttt ccagacgaga
     3541 ctggagatcg gcaaattgca gttcgcgttc acgcaccaga taactgatgc catacagcgc
     3601 tacgcggcga tagtcaccga taatgcgccc acgaccatag ccatctggta aaccggtcag
     3661 cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt
     3721 atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata
     3781 ggcgtggaat gaacttttaa tcatattaat gccaccgaac gggtgtagcg cacgtttcaa
     3841 cggcgcatcc gtttgcaggc caacaatttt ttccagcggc tggttaatat atcccgcatc
     3901 atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt
     3961 ttcgatacgg atgccttcca ttactttttc tcacaattcc gtggtggcag gcgtcgcttc
     4021 ggcgagaaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac
     4081 attaatttcg cttttccagt ccgtaccttt aaagccaagc catgcgtcag cgtacagctt
     4141 atcgctggta tcaatatcta ccttcatgaa aaataatctc tctacaatac ttcaactaaa
     4201 ttatgcaaat tctgcgggcg cgttaacttt acctaaatga atggcatcca aagcaatcat
     4261 tttttcttcg ttagtcggaa taacgacgca aatgacatgc gcattttcac tggaaacaat
     4321 tcgctcgcca aaagagttag atcgattatt catttctgta tcaatctcta cgcctaatac
     4381 agccaaatgt tccatgacca gacgacgaat taaacttgaa ttctctccta ttccgccggt
     4441 gaatataatt ccatccagcc gacgtaatga agctgcatgt ccggcaatat gacgggcaat
     4501 tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt
     4561 ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt
     4621 cactacgcgt tccaggtcac ccaggctctg gttggtttgg ctggcgaccc aggacatcgc
     4681 accaaagtcg acatcgccac tgcgggtacc catcatcaag ccttccagcg gcgtcattcc
     4741 cattgaggtg tcaacactct gaccgttgcg aactgcgcag attgacgcgc cattgccaag
     4801 atgcgccaca accaggccgg agtcatcttc cgccagattc agcagcgaat gtgcgcgctg
     4861 ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta actcttcata
     4921 atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt
     4981 atcaaatacg gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact
     5041 taaatttgcg taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac
     5101 ttcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc
     5161 aattaaggcc acgctgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa
     5221 tgcaccttcg tagctgtgga gagccagcgg tgctggctct cccccattta cggataagaa
     5281 tgcattttcc gagttaatac cgtcggcaat acctgacatt aatacttcac agtcgctggc
     5341 atcgagtacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa
     5401 ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca
     5461 ggatggtcag cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg
     5521 ccagagacgg cgctttacgg atagcataca tcggcaacag gcacagcagg gatgcgataa
     5581 tcggtgcgcc catggcttca atcaggtcga ggatgttcgg gttggcgtag gcaacaaccc
     5641 aggtggagcc catgatgaag atcatgctga gagtattcag tttacccagc gacactttgg
     5701 ttttgtcacc tttataaccg aacttcagaa tcagaccatt caagccttcc agcgtcccca
     5761 gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt
     5821 ccagtgtaat cgcgaacgtt gttttcgtac cggccatgga cgcaaagtgg ttagccagat
     5881 aagaaagcac tggaatattc tgcactttgg cttccgccat gttggccgga gacagagtaa
     5941 acaggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa
     6001 tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac
     6061 gtttagaaac cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa accatgatgg
     6121 aaatccccag ccagacagtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga
     6181 ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca
     6241 ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac
     6301 caaaccagat gacgaaagcc atcagcagca acaggaacag cgccacaaag ccgcgattca
     6361 gcggtgcaaa tccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc
     6421 cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc
     6481 cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag
     6541 aaagacacag acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca
     6601 acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc
     6661 cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg
     6721 acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac
     6781 gaattaatta agcgtcaaca aaaccggtga tttgagagac gcgagaaaga tcgatattgc
     6841 cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta
     6901 ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg cgctgaatta
     6961 aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt
     7021 cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg
     7081 tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa
     7141 cgttttctga ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg
     7201 caataccagc aattaaacca ccaccaccaa ttggcacaat cacgttatcg acatcataga
     7261 gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt
     7321 aaggtgggat aaaaatacga ccttccattt cgacaatttc gctcacttta gcgatagtgt
     7381 cgttgaagtt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt
     7441 tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagt atcgcgcagg
     7501 agagcgaaac cccttgcgca tggttgcccg cagaacaggc caccacgcct ttgcgttttt
     7561 ccgcatcggt cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac
     7621 gctgcatatt ttcaaacttc aggaatattt cacctttgca acgttcacta aaatagttgg
     7681 agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatat
     7741 catcaatagc aaccggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt
     7801 aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta tttatgttta
     7861 aataaaaaca aataactaac caacttctat taattgcctt cgtctacacc cattataaga
     7921 tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac gataattttt
     7981 cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa tagtaataaa
     8041 ttgattagaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga aatcagcatt
     8101 gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt caatactgat
     8161 gccatttctt tgtaacgtag taagcagttc gctgtagtac cccatattag tttgtggcaa
     8221 cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg ttcgggactt
     8281 actggctacc agcacaaact cggactcgaa cagcggctca acatgtaaat cctgaagctt
     8341 catttctgca cttaacgtac caatcgcaaa atccagtcga ccgtcgcgga ttgccggtaa
     8401 gaacgaagac agttgcgctt catacataga aacctgcgct ttcgggaaca cctctttgaa
     8461 cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac caaatgagac
     8521 ttccaccacc gcctcagaag acataccgct tatctcatta accatatttt tcatttcacg
     8581 ggtaatggat tcggaacggg agagtaacaa ttgaccggca ggtgttaatg ttacaccggt
     8641 atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa tgattttact
     8701 gacggccggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc cacttctaat
     8761 gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc tcataacgac
     8821 ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaataaa caggatatgt
     8881 gcgaccactc acaaattaac tttcattact ttccaaggta tcgttattac aattaaatac
     8941 ctttaaatat caacaagtta aagcataaaa atcagcataa accctgattt ctatcaaaaa
     9001 aaaatgacgg ggataaccat attttatggc gataacatca ttcgttatgt ggttaataca
     9061 aaaaaggggc tgagaccata tttcaagcaa ttaccggata attaccaggc aaaatttgag
     9121 ttaaatttaa tgagcaagtt ctcaaatttt attataaata aaccattttc agcgataaat
     9181 actgcggcac gtcacatttt ttcacgctat ttgttggaga acaaacattt attttatcaa
     9241 tattttaaaa tttcgaatac aggtattgat catctcgaac aattgattaa cgtcaacttt
     9301 ttctcttctg acaggacgtc attttgtgaa tgcaatcgtt ttccataaat tcttctcccc
     9361 tcataggcga cgaatagcat tttgtgttga ggatcacaaa acgaataatt gctggtcgcc
     9421 gcgataaggt cagacgaagt caacaagggg gattttcact gtgctttaca tcagcgtagg
     9481 acacagaatg ttgcttcgtt tactgtaacg ccgggtaaat gagcgttttt tgatagtgcg
     9541 taagtgtcgt gcgaggaaaa taagcatcta ttattgtttg tcatgacgac aaggcaggca
     9601 tcaatgaaag gcttcccaat tgcacatatt tttcaccctt caatcccgcc aatgcacgca
     9661 gtggttaaca atcacaatag aaatattgat tattggacgg taaaaagaaa gtttgcagaa
     9721 attgtctcca ccaatgacgt taataaaatt tacagtataa gtaatgaact gcggagagta
     9781 ttatctgcaa ttactgcatt gaatttctat caaggcgatg ttccttctgt catgatccga
     9841 atccaaccgg aaaatatgag tccattcatc atagatattt ctacaggaga acatgatgat
     9901 tatatcatac aaacattaga tgtaggcact tttgcacctt ttggtgaaca atgtacttgc
     9961 tcagccgtca ataaaaaaga gctggaatgt attaaggaga cgatttctaa gtattgcgca
    10021 aagttcaccc aaaaagaagc catcttaaca ccccctaggc actttaataa aacctgcatt
    10081 acttcagatt gttggcaaat tctctttttt tcacccgatc attttaataa tgatttttat
    10141 tgattattta taaaggataa aataatgttc cctgtttcat caattggtaa tgatattagt
    10201 agtgatttag tccgtcgaaa aatgaatgac cttcctgaaa gccccatagg aaataacctc
    10261 gaagcactag cacccggtat agaaaaacta aaacagacct ctattcaaat ggtcacttta
    10321 cttaacgcgt tacaaccaag tggaaaatgc attatcactg gtgattttca aaaagaatta
    10381 gcgtacttac aaaatgtaat tctttataat gactcgtctc ttcgtctgga ttttttaggt
    10441 tataacgccc aaattattca acgatcggac aatacctgcg aacttaccat taatgaaccg
    10501 ttaaaaaacc aggaaatatc tacaggcaat atcaatgtta attgcccatt aaaagatatt
    10561 tacaatgaaa tcaggaggtt gaacgtaatc tttagttgtg ggactggaga tatagttgat
    10621 ctatcctctc tggacttacg taatgtcgat ttagattatt atgatttcac agataaacat
    10681 atggctaata ctattctaaa tcattttaaa ttggattcaa caaattttac taatgccaac
    10741 atgtttcagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
    10801 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
    10861 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
    10921 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
    10981 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
    11041 ttaccagaga tgaaagaatt ctcatcaaaa aaatatatga ttacatcgat acaatcattg
    11101 aagattatga aaatatcaaa gaaaatgctt atctggtcgt cccccaaatt aattatacta
    11161 tggatttaaa catagaagac tctagctcag aagagttaat accctcgaga aagacgaaaa
    11221 ttctccggac aatggctttg aggtcgggga atataacaca tatgaagcat ataactcaga
    11281 gaatcaatat tttaccagag aggactatac gtataattac gaccttttaa atgcaatata
    11341 gacttaataa cttcttacaa agtctattat taataccgta gatattcatc ttaccctgtg
    11401 caccaaaagc aaaaacttat cgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
    11461 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
    11521 gtaggcagga taaggcgttc acgcagcatc cggcatgaat aaagcgcact caacaatctg
    11581 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
    11641 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
    11701 ccgaagcggg tttttacgta tatcaggtga aactgaccga taagccgctt tcttttgggt
    11761 atagcgtcgt ggacagtcat tcatctttct gcccctccaa aagcaaaaac ccgccgaagc
    11821 gggtttttac gtacatcag
//
LOCUS       DAJTRQ010000118        11827 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000118 DAJTRQ010000000
VERSION     DAJTRQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1009
                     /locus_tag="OO332_003285"
     CDS             <1..1009
                     /locus_tag="OO332_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5943655.1"
                     /translation="EKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTKEEL
                     LYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQGIK
                     PLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDIDMKA
                     GTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKVQLR
                     EYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQSRHT
                     YACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVPWSP
                     K"
     gene            1022..1792
                     /locus_tag="OO332_003286"
     CDS             1022..1792
                     /locus_tag="OO332_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5943656.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1845..2240
                     /locus_tag="OO332_003287"
     CDS             1845..2240
                     /locus_tag="OO332_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5943657.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2281..3024
                     /gene="cmoA"
                     /locus_tag="OO332_003288"
     CDS             2281..3024
                     /gene="cmoA"
                     /locus_tag="OO332_003288"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5943658.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3021..3992
                     /gene="cmoB"
                     /locus_tag="OO332_003289"
     CDS             3021..3992
                     /gene="cmoB"
                     /locus_tag="OO332_003289"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5943659.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4157..6586)
                     /gene="torZ"
                     /locus_tag="OO332_003290"
     CDS             complement(4157..6586)
                     /gene="torZ"
                     /locus_tag="OO332_003290"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5943660.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6611..7711)
                     /locus_tag="OO332_003291"
     CDS             complement(6611..7711)
                     /locus_tag="OO332_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5943661.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8099..8845)
                     /gene="cutC"
                     /locus_tag="OO332_003292"
     CDS             complement(8099..8845)
                     /gene="cutC"
                     /locus_tag="OO332_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5943662.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8859..9425)
                     /locus_tag="OO332_003293"
     CDS             complement(8859..9425)
                     /locus_tag="OO332_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5943663.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9641..11374
                     /gene="argS"
                     /locus_tag="OO332_003294"
     CDS             9641..11374
                     /gene="argS"
                     /locus_tag="OO332_003294"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5943664.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11551..>11827
                     /locus_tag="OO332_003295"
     CDS             11551..>11827
                     /locus_tag="OO332_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR5943665.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLN"
BASE COUNT         2930 a         3003 c         2862 g         3032 t
ORIGIN      
        1 agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca atcgctatga
       61 atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt catcggtaac
      121 caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga attcaacgaa
      181 aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca tgacgacaat
      241 cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc ccttccaggg
      301 aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg acgaatttat
      361 tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat tggctgtgta
      421 cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg atatgaaagc
      481 aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc tacctaaaac
      541 tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg tcctgaaaaa
      601 tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac tacgtgaata
      661 tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg ttagaaaaag
      721 caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg aaacagccat
      781 taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt atgcgtgctg
      841 gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc atgcaagtgc
      901 ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag aacagatcgc
      961 aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac acaagagact
     1021 tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg agggcaacac
     1081 cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac agaccacaga
     1141 tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag cattacgaca
     1201 ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc cgcgcattgg
     1261 gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg cgctttggca
     1321 ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc atccacagtt
     1381 tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc gcggcgttaa
     1441 tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg aagctattcg
     1501 cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg cgacaaatcc
     1561 gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat ttcaggtctg
     1621 gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt tacatacgcc
     1681 agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac gtaaaacgct
     1741 tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct gaatttgcca
     1801 cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta agcgcgctat
     1861 atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt cgcctgcgaa
     1921 tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc gctattcgca
     1981 ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg tttatggaaa
     2041 tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt gctggtcgtc
     2101 tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc ggcatgagcg
     2161 ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg ccctgggagt
     2221 tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga tttttacgtt
     2281 atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga ctggaccttt
     2341 gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc cggctattcc
     2401 aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt
     2461 tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat
     2521 gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg ctgccgtcgt
     2581 catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga tattcgcgat
     2641 atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt cctggaacct
     2701 tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg cggcgcgctg
     2761 gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt gttcaacatg
     2821 caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca gaagcgcagc
     2881 atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg cctgcataaa
     2941 gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc actggtggca
     3001 ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg attgccaaaa
     3061 atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc
     3121 agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct gaaattaaac
     3181 cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca ctgagcaccg
     3241 ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc aaagggccgt
     3301 tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa tgggatcgtg
     3361 ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc tgcggcagcg
     3421 gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg atcgacccta
     3481 cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac gatcagcgcg
     3541 cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt gataccgtct
     3601 tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg cagttgaaag
     3661 atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat ggcgatgaga
     3721 acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat ttcatccctt
     3781 ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc cgcattgtgg
     3841 atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt accgaatctc
     3901 tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat cctgcgccta
     3961 aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc ggatgcggcg
     4021 tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg gactatgtag
     4081 gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc gcccgtttcc
     4141 gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt aacttcggtg
     4201 cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag gcatttgcca
     4261 gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta cacaagccgt
     4321 tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg atcccgtcag
     4381 tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc agatcgccat
     4441 tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca cgatcggcaa
     4501 ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg tgtgccggat
     4561 gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca tcaggcgcaa
     4621 gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt ttggagtaaa
     4681 tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg cggaaatcac
     4741 cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt ttattttgct
     4801 gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct ttctgagcgg
     4861 catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag atctcttttc
     4921 cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta cgcgcttcaa
     4981 attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa tcaccggtca
     5041 tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct gcgtgtttgg
     5101 ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc caggctttga
     5161 tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac cagatcattt
     5221 tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca ggattttcca
     5281 gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca gcggcctcgc
     5341 tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca cgcatcgggt
     5401 tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta ccaatctgcc
     5461 ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat tgctggcgct
     5521 gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca gcgagtttga
     5581 ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc ttcggcgtat
     5641 tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg tatttttcaa
     5701 ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct aacatcagtg
     5761 ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag aattcgatgg
     5821 tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc agctgatgga
     5881 agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta ttcagcgggt
     5941 tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag gaggcctgct
     6001 gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct gccccggttg
     6061 aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt tgcagtaagg
     6121 tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca aaaatggccg
     6181 atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt aatgcctgtt
     6241 cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc agtggattgt
     6301 cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt acctgatccg
     6361 ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc gaagaaacaa
     6421 tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg aggattttac
     6481 cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc agcgctccag
     6541 cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact tcctccctga
     6601 tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa taccgcgtta
     6661 aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg gcaatggacg
     6721 gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca gcgcaatagg
     6781 tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat gcgggatcgg
     6841 taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca gcctgccatt
     6901 ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc gccagccgtt
     6961 gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc tgcaacagaa
     7021 cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga ttcacggtag
     7081 ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta gtcactgaag
     7141 tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt tctggcggaa
     7201 aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt gctttgttat
     7261 gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa gaatggcaac
     7321 tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg gccatttcct
     7381 ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat tcatgataaa
     7441 tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt ggaatatggc
     7501 aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt ccctgatatt
     7561 cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc gacgttttat
     7621 gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct atcagcaaaa
     7681 acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt ccagaacacc
     7741 gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc ggcacaacac
     7801 tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa ttgctaagga
     7861 ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa atcccataat
     7921 caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac ccgctcaaca
     7981 gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc cctgttgaaa
     8041 ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac ggtaaaaatc
     8101 atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc ccgtctacga
     8161 tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct tgattacgat
     8221 aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc actccggcat
     8281 cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg attggagcat
     8341 cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct gatttttgcc
     8401 ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta tataaagggt
     8461 tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca gcagccatta
     8521 ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc gtcaccagtc
     8581 caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca aactcaccgt
     8641 cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg atcgtcaccc
     8701 gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct tttggggctg
     8761 cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat tccatgctgt
     8821 aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg ctcgctggca
     8881 acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc ggtgaccgcc
     8941 agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt aactgagatc
     9001 cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt cagcgtttct
     9061 ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta acgtttttcc
     9121 ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac cggttcatgc
     9181 aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag ctcgccacac
     9241 tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg caaagaaatg
     9301 tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc atctaatgcg
     9361 tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga ttgccagttc
     9421 gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc cctgtgccaa
     9481 ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata cgccacgcgc
     9541 acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct ttaactggtg
     9601 cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc aggctcttct
     9661 ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt gcgaaccgca
     9721 gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca tgatggcagt
     9781 tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga ctcatctgga
     9841 tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca acattttcct
     9901 tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt
     9961 tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg tggcgaaaga
    10021 gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc gtactctgga
    10081 gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca ctcagttcgg
    10141 tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa tggagctggc
    10201 tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg aagagtttgc
    10261 cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat
    10321 gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg atcgtctcaa
    10381 cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa tgctgccagg
    10441 aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg cgaccgtcgt
    10501 attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca ttcagaagaa
    10561 agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc gttatgaaac
    10621 actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac acctgatgca
    10681 ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc tggaacacca
    10741 catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac
    10801 agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc tggtggcaga
    10861 aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg
    10921 tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct tcgactggga
    10981 caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt acacgcgtgt
    11041 attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag ctccggtaat
    11101 catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct
    11161 caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt acgatctggc
    11221 cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa acgaagaagt
    11281 gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc tgggtctgga
    11341 tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga gtgaagcctg
    11401 atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga aaacggacat
    11461 gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg cccaggaaaa
    11521 cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt aacattaggc
    11581 attatctctt gccaggctta cgcagaagat acggttatag taaacgacca tgacacttca
    11641 gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa cgtgatcaaa
    11701 tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc ctatggtgaa
    11761 gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga tactgaacaa
    11821 cttaact
//
LOCUS       DAJTRQ010000119        11553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000119 DAJTRQ010000000
VERSION     DAJTRQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO332_003296"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO332_003296"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="OO332_003297"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="OO332_003297"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5943666.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="OO332_003298"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="OO332_003298"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5943667.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="OO332_003299"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="OO332_003299"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5943668.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /gene="garP"
                     /locus_tag="OO332_003300"
     CDS             complement(3367..4701)
                     /gene="garP"
                     /locus_tag="OO332_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5943669.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="OO332_003301"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="OO332_003301"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5943670.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="OO332_003302"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="OO332_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5943671.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="OO332_003303"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="OO332_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="OO332_003304"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="OO332_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5943672.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="OO332_003305"
     CDS             9047..9826
                     /locus_tag="OO332_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5943673.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="OO332_003306"
     CDS             9816..10625
                     /locus_tag="OO332_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5943674.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="OO332_003307"
     CDS             10712..11146
                     /locus_tag="OO332_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5943675.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11553
                     /locus_tag="OO332_003308"
     CDS             11143..>11553
                     /locus_tag="OO332_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5943676.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         2755 a         3093 c         2952 g         2753 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacaaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaataac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggtgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ctaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactggaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cttttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg cat
//
LOCUS       DAJTRQ010000120        11422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000120 DAJTRQ010000000
VERSION     DAJTRQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="OO332_003309"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="OO332_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5943677.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="OO332_003310"
     CDS             complement(2051..2974)
                     /locus_tag="OO332_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5943678.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3191..4534
                     /locus_tag="OO332_003311"
                     /pseudo
     CDS             3191..4534
                     /locus_tag="OO332_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="OO332_003312"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="OO332_003312"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5943679.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="OO332_003313"
     CDS             6554..6778
                     /locus_tag="OO332_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5943680.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="OO332_003314"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="OO332_003314"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5943681.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="OO332_003315"
     CDS             complement(7372..8352)
                     /locus_tag="OO332_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR5943682.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="OO332_003316"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="OO332_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5943683.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="OO332_003317"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="OO332_003317"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5943684.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11029
                     /locus_tag="OO332_003318"
     CDS             10361..11029
                     /locus_tag="OO332_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5943685.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11047..11422)
                     /gene="tnpA"
                     /locus_tag="OO332_003319"
                     /pseudo
     CDS             complement(11047..11422)
                     /gene="tnpA"
                     /locus_tag="OO332_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2951 a         2666 c         2865 g         2940 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgtgtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccctc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg ccgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atacagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagtgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagca gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagactttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgtgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg taaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgctgt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg cactagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aagaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc tgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgtacagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcaggcatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactcgca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcccg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaggaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gcgcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tattctggtt ttttaaacaa ttactctgat ttaaaagaaa caacctcagc
    10501 aacaggtaaa cctgttttac gttgggtaga cccgagtttt gatcaaagca aatatgacag
    10561 catcgtctgg aacccaatca cttattatcc ggtaccaaaa ccttctaccc aggtagggca
    10621 gaaagttctg gataaaattt tgaactatac caacaccgaa atgaaagaag cgatagcgca
    10681 gcgtaaacca gtggttacca ccgctgggcc gcgtagtctg attttccgtg gggccattac
    10741 cggtgtagat accagcaaag aagggctgca attctatgaa gtggttcctg ttgcattagt
    10801 ggttgcgggg acgcaaatgg ctacaggcca ccgtaccatg gatactcgcc tctattttga
    10861 aggtgagctg attgatgcag cgactaataa accggttatc aaagtcgttc gtcagggcga
    10921 aggtaaagac ctgaataacg aaagtacgcc aatggctttc gaaaatatta aacaagttat
    10981 tgatgacatg gcgaccgatg ccaccatgtt tgacgttaac aaaaagtagt tccagacgcg
    11041 ccatcgttag atggcgcttt ttatccggtg cgccgtaaaa ccccatcctt cagggcgggg
    11101 atataaggcg cggttttcca cctgaccagg tgtttgctgt tgttcaatgt attggcggat
    11161 gacggatatt ggcgcacctc cgcagctact ggcaaaatag ccaggactcc acaaaacgcc
    11221 tttgtagtaa tacctgactg caatatctgg tcgatctcgt cgcagtaacc tacccgatac
    11281 gcctttgagg ctgtttacca gactggatat cgccagtttg ggaggttagt taattaacaa
    11341 atggacgtga tctggttcgc catccatttc aaccagttca gcttcaaaat aagcacatac
    11401 atttgaaaag taagtgcgta gt
//
LOCUS       DAJTRQ010000121        11379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000121 DAJTRQ010000000
VERSION     DAJTRQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..1699)
                     /locus_tag="OO332_003320"
                     /pseudo
     CDS             complement(1..1699)
                     /locus_tag="OO332_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(1723..2346)
                     /locus_tag="OO332_003321"
     CDS             complement(1723..2346)
                     /locus_tag="OO332_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5943686.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2343..4031)
                     /locus_tag="OO332_003322"
     CDS             complement(2343..4031)
                     /locus_tag="OO332_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR5943687.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4180..6807)
                     /gene="gyrA"
                     /locus_tag="OO332_003323"
     CDS             complement(4180..6807)
                     /gene="gyrA"
                     /locus_tag="OO332_003323"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5943688.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6954..7676
                     /locus_tag="OO332_003324"
     CDS             6954..7676
                     /locus_tag="OO332_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5943689.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7804..11379)
                     /gene="yfaL"
                     /locus_tag="OO332_003325"
                     /pseudo
     CDS             complement(7804..11379)
                     /gene="yfaL"
                     /locus_tag="OO332_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2639 a         3169 c         2798 g         2773 t
ORIGIN      
        1 acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca accgactcgc
       61 cgctattact gtattgtgcg gcggtactta aagagtaatg cgccaggccg cgttcaatga
      121 ggagctcttt ggtggtggtg acgcgatacg ccgcgccgtc actggcggag acggttaaca
      181 aatagcggct cggtttatcg gcggcgggga gatttaacgc cacataaccg ctggcgtcgg
      241 acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc aaatcgttac
      301 cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt tttaccggct
      361 cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt ttgaactctt
      421 ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc acgcgaaaac
      481 tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg gcaacggcat
      541 tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg acatcgacgg
      601 tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg ctgacgatgg
      661 gggatgaatg caacggatcg cggaactcgc ggcccatcac tttaacatcg acacgatcgc
      721 ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag atttcgcttt
      781 cgtagaagaa gttctcggag acaaaaacgc cgccttcagc atccttaccc agaatgtatg
      841 aacgttctgg cgatatatgt tgtaactgca aagtaccgct gtcatcggtc accccgcggg
      901 tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct tcaccctgtt
      961 ttttacccgc ggtccatacc agaagctcgt tacctgacac tttgctaagc gccacggtat
     1021 cggaaacaaa caccaccgtc gtcgcccgat acccacctac catcgcctcg acgaggtaca
     1081 gccccggctc ttgttggccg agaggaatat aaatattacc cggctgcggc gagatgaaat
     1141 tgctggatgc gccttccagt tttatccctt gctgcggctc gaccggttta gcctgccata
     1201 gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac tggttgttct
     1261 gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt aatgcctgag
     1321 tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga gatttgccgt
     1381 accagttatc ccacagctag gtcagcgtat tgttcagccc gtcgcccaga tattgcggtt
     1441 gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg tcaggaatac
     1501 gatacaggcg aacgtccacg ccgccgtact cttccatctg ataacgccga taatcacgcc
     1561 ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg tcagcaagca
     1621 aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa tcatcagctt
     1681 tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg ccagaaaatt
     1741 taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc gggtgtcctt
     1801 ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt tcgtggcgct
     1861 tccggtgtgg ttgatgacgt aacgccccat ccagaccatt aagtgctggg catcgccctg
     1921 atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcttggccaa taaactggct
     1981 gttgtactga atcaaattaa tcgcggtcac gtaggggccg tttttcccgc tcccctgatt
     2041 ccagttttgc gccagttgac gctgttcagg tgttagcgtc atctctggcg gcaaatactg
     2101 gctagataaa ccgttacttt ttagccattt actgtcgtga actttcagcg tctcgttcgc
     2161 cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg gcccctggcg
     2221 gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt gttcgacatt
     2281 cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca gcagcccgtg
     2341 tctcatagcg gctgccaggt tatgggtagc cactgccagg cgacaccggg ttccatctgg
     2401 gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg cattaataaa
     2461 gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt caacgtttcg
     2521 ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat gccatcagtg
     2581 ggtattacat ccaccattgc cgggcgggtt ttattcagtg tttgcagggc gttattaacc
     2641 agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga cacacggaaa
     2701 aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata ctgaccgtat
     2761 cgggaactca cttcgcgaca ccagatttgc gcttcgccct gctgagtctg gcttaccggc
     2821 aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa cagtttccct
     2881 ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg ggtttgcaat
     2941 tttgagtctt cataccagca cagccccacg gcaccgtcta acgccccatt cagcttgtcg
     3001 ttttcctggc tgatgtgcga aagcatctct tcggcaatac cgtgtgaata cggcaccgcc
     3061 acacagaagc tggctccggc aggcatactg ttccagaccg gcgtaaaatc gaaactggca
     3121 tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc gttacccatt
     3181 tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc cagcaacctg
     3241 gcgctggcca cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc ttccaggcca
     3301 aagctggctt gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc cgtggcttcg
     3361 gtatcctgct gatcatcttt aaacaacata tccgtactgg aaaacaccag catcttgtcc
     3421 tgatatgtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata aacaggaata
     3481 gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct gtcgctggta
     3541 gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat cagcaccatg
     3601 taatgtgaaa gatggccttg tttatcgtgc catagcgccg cctgcgcggg ctgatctaag
     3661 agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat gcggcgaatg
     3721 ctgccttcaa tgcccagacg atctgcatga ttctgataat agaaaacgaa atcctcgctc
     3781 agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct cagagagtcg
     3841 ctgtctagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg caccaccagc
     3901 ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac gccagcggaa
     3961 agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt cgcctttttt
     4021 tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg cacagccaaa
     4081 ctttaccgcg ccctaatacg acaaaagccc agactttgca gcctggactt ttcaattcaa
     4141 acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt ctggctcgtc
     4201 gtcaacgtcc acttccggag caatttcatc atccccttcc gcggcactgc cgtcgatggt
     4261 atccagatct tcctcgtcaa ccggttcagc aacacgctgc agacccacta cgttttcatc
     4321 ttccgcagta cggatgagga tcacgccctg ggtgttacgg cccacgatgc tgatttctga
     4381 aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt cgcagtcatc
     4441 tacctgcacc gcgccaacca ctaaaccgtt acgttcggta accttgatgg agataacccc
     4501 tttcgtcgca cgcgacttgg ttgggtattc cgccactgca gtacgtttac cgtaaccgtt
     4561 ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga cgactttatc
     4621 gccttcacct aagcgaatac cgcgcacacc agtggtattg cagcccatcg cacggacaga
     4681 agactcttta aagcgcacca ctttaccttc ggcggagaac agcattactt catcttcgcc
     4741 actggtcaga tcaacgccga tcagctcatc gccgtcaacc agtttgatcg ccactttacc
     4801 ggcggtacgc agacggttga attcggtgag gacggttttc ttcacagtac cgttagcggt
     4861 cgccatgaag actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg cggtgatacg
     4921 ttcgtcctgt tccagcggta gcaggttgac gatcggacga ccgcgcgcgc cacgagtggc
     4981 ttccggcaac tggtaaactt tcatcgaata gacgcgacca cggctggaga agcacagaat
     5041 atggtcgtgc gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt taatacgtgc
     5101 ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg gctgatattt
     5161 aacgtagccc tggtgagaga gcgtcacgac cacatcttcc tgggtgatca gatcttccag
     5221 gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga actgttcacg
     5281 aaccagctcc agctcttcgc ggatcacttc catcagacga tcggcgctac caagaatacg
     5341 caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt tttcgtgctc
     5401 aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct gttcggtcag
     5461 gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac gcgcagcatc
     5521 gtcgccagca cgttccaaca tggcggcaac gttgcccagc tgccacggat tagcaaccag
     5581 cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga tcgggtcaat
     5641 gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag ctttacgcag
     5701 ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg ccgcgatgat
     5761 gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt tgataccgaa
     5821 agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac cgaccgcatc
     5881 gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac gcagcgcgct
     5941 gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca ggcgcgcttt
     6001 gtttacctga tacggaattt cgtggacgat aatggtttca cgtccggttt tggcgtcaac
     6061 ttccacttca gcacgggcgc ggatatatac cttgccgcga ccggtacggt aagcttcttc
     6121 aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg ggatgtgttc
     6181 catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac cgttgatgac
     6241 ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac cggaagaacc
     6301 gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt ccgtgccgtc
     6361 atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt catgggcaat
     6421 tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt cgatggaacc
     6481 gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg ccatacggac
     6541 gatcgtgtca taaaccgccg cgtcaccatg gggatggtat ttaccgatta cgtcaccaac
     6601 gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt tcatggcgta
     6661 aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg cacggccaac
     6721 aatgaccgac atcgcataat ccagatagga gctctttagc tcttcctcaa tgttgaccgg
     6781 tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta tcccggattc
     6841 aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg ctttattcac
     6901 atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg ccaatgaatg
     6961 ccgaaaaatc gccggaaaac cataacgtag accatgaaga gatcgctaaa tttgaagccg
     7021 tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc attaacccgc
     7081 tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag gtgctcgatg
     7141 tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg acggtgaccg
     7201 gtctggatat gggctttgag ccattgcagg tggcaaagct gcacgcgctg gaaagcggca
     7261 ttcaggtgga ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat gccgggcagt
     7321 atgatgtggt gacctgcatg gagatgctgg agcacgtacc cgatccgcag tcagtggtca
     7381 gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg cttaaccgca
     7441 acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc atggtgccca
     7501 aaggtacgca tgatgtgaag aagtttatta aaccggcaga gctgcttggc tgggtggatc
     7561 agaccagttt aaaagagcga catataaccg ggctacatta caacccgatc actaattcct
     7621 ttaagctcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat aagtgaggtt
     7681 gatgtttggc cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag gcctacaaat
     7741 gctctccgta ggccagataa ggcgtttacg ccgcatccgg caattgtgat ggtcagacag
     7801 cgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat catcactccc
     7861 tttctgccac gccacgctgc cgcgcagaga cactcgctga ctgatattgc ccgtgactcc
     7921 cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc catcttcttc
     7981 aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg tgacaccaac
     8041 cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc cctgcgattg
     8101 tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct gataaatcac
     8161 ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc cggcttccag
     8221 cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt cggaaacatc
     8281 attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct tctgattacc
     8341 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8401 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     8461 aagcatccac tcgccatccg tgccccagca cccgctaaac aagccgccgc taagcagcac
     8521 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     8581 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     8641 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     8701 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     8761 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     8821 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     8881 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     8941 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9001 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9061 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9121 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9181 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9241 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9301 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9361 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9421 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
     9481 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
     9541 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
     9601 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
     9661 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
     9721 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
     9781 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
     9841 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
     9901 cacgtagacg ccatctttcc cgtcaccgcc gatatgcaaa ttgtcgccac ctgcaagatc
     9961 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10021 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10081 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10141 aagcaccgca ccatcatcaa cgactatatc tccggttagc gccatcgact gcgcccctgc
    10201 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10261 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10321 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10381 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10441 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    10501 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    10561 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    10621 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    10681 atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg cgctattgtt
    10741 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    10801 atagggagcc ctcacgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    10861 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    10921 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    10981 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11041 acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac gtaaatcgac
    11101 ggcaccagta tcattagtac cagaagaata gattgcgcca ctatagccgc ctgcgacgtt
    11161 accggaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa atatggcccc
    11221 gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc cagtttcatt
    11281 ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggccccac cgctggcatt
    11341 attggtcata tccgaaaaaa ccagccattg cccatcggc
//
LOCUS       DAJTRQ010000122        11228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000122 DAJTRQ010000000
VERSION     DAJTRQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(88..427)
                     /locus_tag="OO332_003326"
                     /pseudo
     CDS             complement(88..427)
                     /locus_tag="OO332_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(743..1387)
                     /locus_tag="OO332_003327"
     CDS             complement(743..1387)
                     /locus_tag="OO332_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943690.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1408..1677)
                     /locus_tag="OO332_003328"
     CDS             complement(1408..1677)
                     /locus_tag="OO332_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5943691.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1756..2394)
                     /locus_tag="OO332_003329"
     CDS             complement(1756..2394)
                     /locus_tag="OO332_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5943692.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2379..3611)
                     /locus_tag="OO332_003330"
     CDS             complement(2379..3611)
                     /locus_tag="OO332_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5943693.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4112..6793)
                     /locus_tag="OO332_003331"
     CDS             complement(4112..6793)
                     /locus_tag="OO332_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5943694.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(7034..8080)
                     /locus_tag="OO332_003332"
     CDS             complement(7034..8080)
                     /locus_tag="OO332_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HCR5943695.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8184..8333)
                     /locus_tag="OO332_003333"
     CDS             complement(8184..8333)
                     /locus_tag="OO332_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943696.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8755..8946
                     /locus_tag="OO332_003334"
     CDS             8755..8946
                     /locus_tag="OO332_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5943697.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMRDGYPHPCM
                     QPMYNFLGKFKPEPREETE"
     gene            complement(8999..9232)
                     /locus_tag="OO332_003335"
     CDS             complement(8999..9232)
                     /locus_tag="OO332_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943698.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9328..9951)
                     /locus_tag="OO332_003336"
     CDS             complement(9328..9951)
                     /locus_tag="OO332_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5943699.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(10040..10549)
                     /locus_tag="OO332_003337"
     CDS             complement(10040..10549)
                     /locus_tag="OO332_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943700.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            11007..>11228
                     /locus_tag="OO332_003338"
     CDS             11007..>11228
                     /locus_tag="OO332_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943701.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSL"
BASE COUNT         3122 a         2829 c         2257 g         3020 t
ORIGIN      
        1 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
       61 actctccagg aaacccgggg cggttcaaac ctgattctgg aaaaggactt tgtggattgt
      121 tgctattgcc agtgagattg aatgaagtcg ttttaactcg ctttgaatac gtcttgcccc
      181 cagatttcgc tgagaacgta actccagaat cagagcgact tcgcaagtgc cggttttcgt
      241 tgatggtgaa cgcttggggc gacggctgtg gctttccaga ccagcgatgc cctgagccag
      301 atatctgcgc caccatttta cgcaatgtgg gccgagaaat gccacagcga cggcatacaa
      361 atcccgcatc accggacgtt tcatagagtt ttacccactg tagccgctgc tgaatttccc
      421 tgttcataat ttggcattag ttcagctcaa tcccgactta aaaagtccct acaggatgtt
      481 ccggacctga ggcacagttc atatccttat ccataaaaaa tttagctatg tctatagcat
      541 taatcttgac aagacgacgt tagggcctgg cgatgaatca tttcccctgc cagatatcct
      601 ttggggtgca ccagaactac cgttttatat ctatataact gaaacgtccc tcgccatcct
      661 tcatattttg atgtagtaac accattcact ttcatctacc ccccgtttta ttaacatata
      721 gtaaaaatca cctcgccgca ttttattctt ttgaagtaaa atcatttacc agttcactta
      781 aagctctctt ttgttcatca tcatacagag attgaatccg gaatttaatc tcttctgcaa
      841 ccagtcttgc atcgggctca taaaatattt ttgttattcg ctgtttctga tgttcaggct
      901 ggtcttccat atttcgtaaa accagatgaa ataatatatc ataaagtgcg caatgccatg
      961 aataaccatc attggttaaa ataagctccc aaacccgctc ctgctcggca ataagctgca
     1021 gaacaaagga ctcaacctgt tctgtttgtt cgggggcaac acggtatagc ggagcaatca
     1081 cagcaaaaga tagttcattt aaccaggatt ccgcttcctt ctcgctcctg aacggtggac
     1141 agttttgtat tcttcgttcg tattcacggg taatctgctc cagaatatcc tccggcggtg
     1201 tcagccactt attcctgagc aatacactgg ttgatggatg acagctggct atttcagcaa
     1261 taatttcacg taatgtttcc gggctgaact tcttcagctg attgtagtgc tcccgggtaa
     1321 aaccatgttc atcgcataaa agttgccatg tcagtgatat actgtttgcc attccttgct
     1381 tcatcattaa tacctgttac ctgaatatca acagaccggt gtaccttttc tgaatacctc
     1441 cccggacgga gtggcaatcc ccattcgcct cagcgtggca tctgcagctt tcgccacctc
     1501 gctgatatcc agcttttcag gtagcggcgg ggacgatatc tgcgccagac aataattccg
     1561 gcgcaacaac aaccactccc gttgtgcttc tgtacattca acatttccca taacaaaaag
     1621 ccagcggtca gaagctctgc gccagcaacc agcagcctcc agtttctgcg caaccatgtc
     1681 ttccacaact tccggggtac tctttttcct gctcaaaaca ttaactcact gaaataatat
     1741 acatatctga cggtctcagc gaaccgtcca tgccgggctg aaatcctctt cctgaaacag
     1801 ctccgtcagc ccgctaatct gtttcaatct gagaacttca tcctggtcca tacccagctc
     1861 cgtaccaatg cgttcattcg tccagcccag gcgcgacagg tccctgacaa tatcagacat
     1921 tgaggttatc tgatgtttgc ccctggcccg gttatgtcgg atggtcgcag caatacgtga
     1981 ggcctgtccc tttctctccg gattaataca gaccaccggc agccagccct tcagacgttt
     2041 tcccgtacct gcttccctgc ccagcacctg gcgatgaaaa ccatccacca ccaggtaatg
     2101 gttcttttcc tcagacacca ccaccggttg tgtgaaacca tctttctcca gtgactgctt
     2161 cagcagtttc ttctcacccg gcgccatgac attaggattg taatcattgg ccaccacctc
     2221 atctgctttt acccacagca cacagtccac tggctcatgt gcaaaaggac tgacttcatg
     2281 aagcactctg cgcaggtgat taagggcctg tatggtctct tcttctggtc gttcctggct
     2341 gaagtactga cgaatcatgt tgccaattat ctccggtgtc acagaatccc ccattccttc
     2401 ctcctttctt tcatacgctg cagataacgt tcatagtgcc ggggtttgtt cggactgaag
     2461 gagagggtcc ggcaccagaa atcattcttt ataagtgtct tacagatacg ccgccaggac
     2521 ggaatatccc ggctccccag gtcattctcc tgttcatccg ggatgtcatc cgggaagccc
     2581 cgcgtctgat accagcgcag gtagacagca attttattac ggtaatgttc tgccgttctt
     2641 tccggcatca catccagcag gaacatcgca tagctgcgcc aggtatgatg ggctggtttt
     2701 gatatacgtt tacgcagggc aaaataggca ccgctttcat tggcataaag cgccccgctg
     2761 gcagcgcctg acacccgctc acacatcctg gcccaggttt ccggctccag aatatggtaa
     2821 agccacagcc ccttacgttg ttcaggacca aaaggttcac agacacgcat gttgcgtaac
     2881 ggcacgtcgg cacggtacat caggtcatac agaggattgt aaatggcaca agctctggcg
     2941 ttatatatcc agatatcgcg ggctttccag tcatacaacg gatacatggt gtaataaaac
     3001 ccttcaggcg atgccgtggt ccagggctta tcatcggcat atctcagttt gcgctgagac
     3061 accagcccca taaagcgatt gagggattca tccgcacgaa caccggtcag cactgccact
     3121 ccacaccggt taccggcaaa ccaggaagaa aatgccggaa caaattcttc aaacgtcatg
     3181 gcataccgga aaaagggaaa atatgtcata tccgtaatgg cctcttccgg tggctggcga
     3241 acccaggtca ctcctggctc ccagcatatc cactccggct gaaactgcga gacgccgttt
     3301 accgtggtca ggggaagtgc cacccagtaa aaggtttccg tcacatcatg gtacatttcc
     3361 cgcatcttct gaatatgttc aatggtgcac cgatactggg cctcccagtc aatgaacaga
     3421 acagagaaat gacgttttct cctgcgggcc acctctgcca caagatggaa aagcacggtg
     3481 gaatcctttc cacctgaaaa agacagacat accgacggaa atgtttcaaa aatccactca
     3541 atgcgggcga ttgcggcacg caaaacattt tctccggtaa ggattttttc ggttaaaagc
     3601 gtatcactca ttttcctggc tttatgtcga ataattctgt ttatacattc aggcaaactt
     3661 cattaataac tcaccgcttt ggggataaat aataacaaca aaaatcttca gatattaacc
     3721 tggttgttgg atttgttatc acatccttta tccacctgct tcttactggt aacacatctg
     3781 gaaaatccat gcattttcaa catatactat cctttcaact tgtaaataag aaaatatgat
     3841 gcagatggtg aacaattcaa atatcatttt gcaggaagta ttcactgtaa tagaattacc
     3901 ccctgataaa gtttattcac taaggaatta ttcaacagaa acattccctg ttagctttga
     3961 gcaatagcac ttatttttat atgtatcagg aatacgtacg ttgatgtaac caaaacaaag
     4021 aagcctgtgt ccatggggtt cgtacaattg tgtgtggcac atgaccaaat caggtttgag
     4081 acctcccgct gcaatatttt ttattcagat tttagcctgt caggcctgac agaaacgctc
     4141 ccaacttccc ttctatatca ctgtctatag tacgtaatct tagcaatggc aggccgcatt
     4201 tcttcagtat actgttttta agttcatcac gttcagcctg aacactggtc agatgataac
     4261 ctccatccac ttcaataacc cctaatggag tcttcccgac cttatagtaa acaacaaagt
     4321 cacaactggc ccggttacgc atgaataact gttcacggtg tgtaaaatcc ccccgctcca
     4381 gcaacaccaa ctgattcagc gctatttgtg aatgaaacat catactccga taactatcct
     4441 gcgacaggat atcccgcaac aggcaggcca caatctgttc tgacttaaag tgtgaatcat
     4501 ttgaattcaa tctactattc agtcgttcca gtgatttgtc atactcactg tacagcaaat
     4561 caaatgctga tatgaccggg ctctcgaaaa tctctccgtc atcagcgtag tatttgatgt
     4621 aacgaatcag cgccgcaata tgtccagcgt ggcgttcaaa tacatcatta ccagtcacca
     4681 gcgtaaattt atttcgggcc cttgaaacag cgacattcac cagttcagga ttatcaacaa
     4741 acgcgatgtt ccgactgtgc tggctgctgc gcttcttatc cagcacagtg gaaaacacaa
     4801 tttcatcgca ctcccgtccc tgaaatttat gcaccgtcga tttcacaaaa tcagcaggca
     4861 gcactttttc agccagatta acctgtgcat tatatggggc aatgtatccc ctgctacttt
     4921 cttcatccca gtaatgacct tccagagatt caagctccct gagatttgaa aaattacggg
     4981 tatggtttcc ttttgccgtg atgaccagag ataatgaggc ttcaccggaa tcaaccgtta
     5041 acggtatcag agcattatca taaaactgct tattacaaaa ttgaatgatt ttaggatgac
     5101 aacgataatg ctctttaaga agggttactg gcaccatgtt cctgaacaac atacaaaccg
     5161 aatcaagtag actatatttc tcacaattat agtattctgc cggtggagat gggctgttcg
     5221 ggagtaatac aggaacatgt ggaagttgct tacgatcccc tacaacgatt acattacggg
     5281 cacatcccag tcccagaatg cccggaacaa tatcctgctg cgaggcttca tcaataataa
     5341 cataatcaag aagggccccc ttgccgatag agttaataat cgaatgcgta ctgctaccaa
     5401 taatcggaaa acgctttata aaacggtcaa attcatccct gtatgtctct gccgagaatg
     5461 aacttcttgt tgaaacattt cttcgcagat gttgctttaa ataaagcata gaccatgatg
     5521 tcaggcgccc cagtaaaatt ttaaaatcag attttttaaa tacttcctca tattcattca
     5581 gagaaatttg tttttcccgg atattttttt cataataaga aagctgaaga gaagtaaata
     5641 gcgccagcct tctctccgga atcatcaatg gtttgacttt cagtatcatg aagtttaaca
     5701 acaaatcaat tctgtttctg aatccaatcc gcctgtcaga aaggtaatga atataagcca
     5761 tcaggtcggt tgttttttgt gaggaaaatc ggtatttgtg agaaaaaagc tcctccggtc
     5821 gaagattctg actctgccat tgctgcaggt atttatactc aatattcagc tcattaattt
     5881 ctgcttttaa cgaagcgacc tggttaatgg cattcaggtg ttttttcact tgttgaagta
     5941 catcatcaat cgcatttgca gacggtgagt caggtaagac ttcctcagaa cgcgaaatcg
     6001 acgttgaaaa aaactgttgc ctgttctccg tactccccag tcgtgccatc acatatccca
     6061 gttgctgttt gtccatcttt tcataaacat tactgacagc tgagttatta ttggacaaaa
     6121 ttgccactgt tttattctga attaatatat tagcaacaat attgagtata gtctgtgttt
     6181 tacctgttcc aggtggtcct tcaataactg aaatctgtga ggaaaatgcc cgtttgacag
     6241 ccagcaactg actttcatta agaccaaacg gaaaaatcaa accccatggc gaatcacgtt
     6301 ttttactctg tcctgaacag tatgcatgca aggcggtatc cggatgagga actattttct
     6361 ttatctgcct aaggatattc tcatcgatat ttctgttatt accacgggca taaaaaatac
     6421 gttcattagc caccctacac aagtaattaa acacggcatg ctctgtaaat gctgaacact
     6481 gttgaaagtc aaggccggtc gttttcatga tgtagggtcg ttcaccctcg cggtaataaa
     6541 cagcagcata tttatcacca tatataataa ccagttcagc ccgggatgtt acagcgttcc
     6601 ctttcctttc gaggaaaaca tttcgcaaat ttacagttgg cgttatctga catacagaaa
     6661 gcggacgagt atattttctc ccgctgcgaa aatgacaggt cagcataacc tctctgtcgt
     6721 catttccagc ccacagcgtc cagtcggtaa ttttatgagt cttattttca ccgtcaacac
     6781 aaatcgtgac catattctca ctcagaaatt atcatttaac ttacggaaaa ttgttaaggc
     6841 actcccgata ttagctccgg aggattttcc acaactccgg gactgttaac atatttctgc
     6901 ctgccatata tacattgaat ttagtcataa cagtcaatac cagaattaca ggaatgcgat
     6961 gccgatacgc tggttattga atactggtag cccctgagca ctaattcatc acgatactct
     7021 gtatttactg ttttcactgc tttttacaac cattccccca ggtaaaatgc tgctcctccg
     7081 gtgatggctg tgtcagaaac tgccatcccg gaatagccac gctgaccgcc agcaatgccg
     7141 cacagacacc agccacaaac ggtttcagaa acgtgggcgg ggatttctga agggagggtg
     7201 actgctccgc ctgcactttc cccttgcgat tatcaaggct cagtgcatcc agacgcaccg
     7261 cgccatttgc cgcaagcagc ccttcccggt tatccacgct tcccggcaga ctcagcgaaa
     7321 aatcccctgt tcccgtctga atcagactgc ctttacgatt tgacaggctt tgtgccgata
     7381 tctgcagtgt gtcggcattt atcgtaccgc cattactgct gaactgcccg gcagtccggg
     7441 cggaaagcgt accggcactc agttgcgcat tctgtgtaga cagatttcca ctggttgcat
     7501 tcagctcagt atgttgtccc tgtatccggc tgttacctaa atccagcccc tgctcaatgt
     7561 cccgtaaatc cagcagagaa ccttcgtgtg ccgggaatga ggaatacaac tgaatatagt
     7621 catcactgtc cggtgtcaga cgcacatgtc gcacaacacc gggaataatg accagccgga
     7681 ggataccgct ttttaaatcc tgcgaaggtg ccagaacacg ggtggtgaca taaccatgat
     7741 cgaccagacg gttctgcaat gtactcatca gcagattaat tcctttcgcc cccaggcaat
     7801 gcccgaccgc cccgttggcg attttttgta aaggcagcca gtgtggtaac gcatcagccc
     7861 ccttcagttc cgtctgttta atctgaaaac agggcgtttc aacaggaaaa ttaatcttat
     7921 gggcaaaaga gccaggtgca gaaaggcgaa catcaggggc tgaaggcgtc agttgttgct
     7981 caagggcttc ctgacgctgc tgctgaatga taaatgcttc atctccgcta cgccggacat
     8041 ccgctgcctg aagagataaa ggcgataata aagcagacat cagtgtcagt gacagcaagg
     8101 acggacagcg ccggaataag ctttgttcct cagtcctctc gttcctgcaa cataattttc
     8161 cagtcaaacg aaaaaccttt tattcatgtc ataccaacaa tcaccagcct cctgacgata
     8221 cctcccacca tgttttgcga tggcacgcta ccgcccttcc ggtatttacc cgcatcaacc
     8281 ggtaccagaa tgctgagtaa caaaccagcc agaattgact taattttgat cattatagaa
     8341 taatgatctg aactacagca acatgacaga tttatcgatc gatataacac aaatgaacag
     8401 ttctttattc tgccatatat cgcaaaaaca gcacattaca aaatttataa ccataattac
     8461 tggaaaagtg cagaaaatta ttccactgaa tgcttttaat acagttggtt agcttaaaaa
     8521 atagcaattt ttgatttcag tcattgaaag ttgcaatgtt tgacgattca acagtattta
     8581 agtgagtttc aggatatggg aaggacatga aaggaggagc gcaataaaat acacaggaag
     8641 aaggggaaaa tgtttcgtaa aaacagaaaa ttaacagcgc acatcaggaa acgcagtctc
     8701 ctgattcagt gctaccacat gaaaatttag ttaactgtta ccgtactaaa atttatgtca
     8761 gaagatgttc cattaccgaa agtcaatcag cgatacaaag acgatcatgg tgcgcttgtc
     8821 accctgacca gtgttgagga aacccgggtg gtgtttatgc gggacggcta tccgcatccc
     8881 tgcatgcaac cgatgtacaa ctttctggga aaattcaaac ccgaaccccg ggaagaaaca
     8941 gaataaaacg aaatccctgc cggcaatcac actacctgat agcagcataa tccacttatt
     9001 aattctcatc cccggagaca agcccagaaa accactccgg gtgttcactg tatgccgtgt
     9061 tcagcgcatc cctggaaacg tttatataaa agctgtcctg caatttgcgg gtaacaactt
     9121 ctgccaggcg attccggtta tccaccagtc cggcataacc tctctctgcc agccactcat
     9181 tcagttcatg ttccagagac agattgataa tatcataatc ggaccttgcc attcccgttt
     9241 ccttagcaca tgttttcagg cacggggata aagtataaag aatgtcatac tgctgctgcc
     9301 agagcaatat tccccctgat ggttgtatca gagatggtat gccggtactt tgcgggttgt
     9361 tcccgtcagg ttatcgtgca cctccacggt cgtggtcacc accggcattc cggcctttct
     9421 cagcctcaga acatcagccg caatacgctg actgccaaac cagaacaggc cgtccagtgc
     9481 agtcaccagc aaacccgcct ccagtgcata cttcagccgt tcacggggcg ctttcacttc
     9541 ccgggcaacc tgctgatatg gcaatgatgt gttttcattc ccaaccacgc gacgaatacg
     9601 gtgagacaga tgataccggt atgtatccgg cacaccagaa aggctggcct tcaggctgta
     9661 cacgcagcca aaccgtttat cgttaaacac cacatttttc tggctgatgc cccattcttc
     9721 acgcagcgcg gcaatcaatt gcggtgtacg ggtaagcagc aagcgaaaag gcagttcaaa
     9781 actggtgacg taatccacat tcagcagtgc aatacgcaac cgttcttctg gcccggcctc
     9841 tgtctgccgg cactcctcca gaacatcctg ccactgccgg cgaagacggg gggattcgtt
     9901 ccgttctgta aaacagtatt tatccgccag atagtcaatt cgtgtacgca tactgaaagg
     9961 aattccgtat aaagattcag cagacaaaac tttatcagcc agtaaaaatt aacggaagca
    10021 ttgatattcc tcccgacaat caccggatga ttgttgcaat aacctgcgac ataaaggact
    10081 gaacagccgt ttttaacgcc acgtattgcc ctgatgaacc aggccgggca acccgaaaac
    10141 atctttccac ccggcattgt ccgccagcac ttatcaccga ccaggtctgt tgcagtaaat
    10201 ccaccagttg cgcacattct tcatcagcca tgacgccgac tcgcggatgg caaatactcg
    10261 tctggctgag cagcaacaga catcgtatct gtgctccttc atgttgttcc cgcaaccagc
    10321 gtaataccac cggcgctgat gggctgaccg tgtgattatg ctccgtaata cgttcgtgta
    10381 cccgtcggtg aaaggaacta caaaatgtct gaatctgttg accatagatt ccttctgccg
    10441 aatagagcgt gaagtgaaat gccaacagat ttggccagtg gtccaccgcc tgctgaacaa
    10501 aacgccggat ttcccccggc tctgaaatta aggcttcggt tatttgcacg aaatgttcct
    10561 tttcaacccc tgttgaattt tgtcaagccg gtgcagacac agtaaagcgc gcatcaacac
    10621 ctgaaaggta cgatacaaac acaagttaca aacaataaga acggaaggac aattaatcag
    10681 tgaggaataa taactaaatt aaccaaaaga atgttactga caatactacc aatacttaaa
    10741 tagcaatata ttaattaatg taagaacaga tgcccggtaa gacatataca caatacatcc
    10801 acaaacacac tcttttcatt aaatcaatca cctgattaaa ccatgaaagt gtgatgtaag
    10861 tcaaaattac tgatactatc aattgataat gattatctct atcattatcg tgaaattcga
    10921 gaatgaaagg agaagtagtc attataagcc ttttataaat cgtcatcatc atagtaaagt
    10981 agaacacttt ttatatgagt tactatatga aaatatactg gacaagaaaa cgtattcctg
    11041 aactttcagc acttccacct tcattaagaa agaaaaattt tactgacgca tataatgcag
    11101 catcttctca tatcgaatac tggataggtg ctggcgtctc atttatctcg atgatgattt
    11161 tatccagagt atatgacttt cttttacccg cccaagatac tttcccgggt gatatcatca
    11221 gaagttta
//
LOCUS       DAJTRQ010000123        11200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000123 DAJTRQ010000000
VERSION     DAJTRQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            34..393
                     /locus_tag="OO332_003339"
     CDS             34..393
                     /locus_tag="OO332_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5943702.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="OO332_003340"
     CDS             complement(513..692)
                     /locus_tag="OO332_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5943703.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="OO332_003341"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="OO332_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5943704.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="OO332_003342"
     CDS             2131..2709
                     /locus_tag="OO332_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5943705.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="OO332_003343"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="OO332_003343"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5943706.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..5986
                     /locus_tag="OO332_003344"
     CDS             4388..5986
                     /locus_tag="OO332_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5943707.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="OO332_003345"
     CDS             complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="OO332_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5943708.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8003..8974
                     /gene="manX"
                     /locus_tag="OO332_003346"
     CDS             8003..8974
                     /gene="manX"
                     /locus_tag="OO332_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5943709.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9037..9837
                     /gene="manY"
                     /locus_tag="OO332_003347"
     CDS             9037..9837
                     /gene="manY"
                     /locus_tag="OO332_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5943710.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9850..10701
                     /gene="manZ"
                     /locus_tag="OO332_003348"
     CDS             9850..10701
                     /gene="manZ"
                     /locus_tag="OO332_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5943711.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10756..>11200
                     /locus_tag="OO332_003349"
     CDS             10756..>11200
                     /locus_tag="OO332_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5943712.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2749 a         2829 c         2827 g         2795 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgataatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac agcggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg ggggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5701 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5761 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggagg gcgtggaaac
     5821 gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag ggtatttgta
     5881 cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc aaccgccgcc
     5941 cgcccggcat aatggacata tcacgcccat tatgtcgtta cgttaacgct tactcatctt
     6001 catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa tcattggctt
     6061 caaggatggt gatatgcagt ggccctacat caatcacatc gcccacacgg ggaatgtgac
     6121 catttgccga gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct
     6181 caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc agccagccgt
     6241 taccatcggt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt
     6301 ccagcacatc cagcggcgtg accaagcctt gtaccacacc aaactcgttg gtcacgataa
     6361 caaagctccc gcgagcgcga cgcagcacgc ccagcaggtt gatcggatcg agggtttccg
     6421 ggacgataat cgccggagac gccgaagcaa tcgccgccac atcaacgccc tcttccagcg
     6481 ccaccagcag ttctttagca cgtacgatac cgatgatttc atccagttca ccgcgacata
     6541 ccgggaacag actgtgcggt gaagagagca gttgctcgcg gatttcatcg accccgagat
     6601 tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg
     6661 ccagcgtcag tacgccgtta atcatgtacc gttcttcttc ggcaaatgca ccttccggga
     6721 tcggcatcgg gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac
     6781 gcaggatggc atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt
     6841 tgcgacgcgc aatctggtta aacacttcga taatgatcga gaagccaatc gcggcataca
     6901 ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta
     6961 acaggaagct cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg
     7021 gtttcgatgc cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca
     7081 gatggttaac catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga
     7141 tgacaatctg tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat
     7201 gatcgcagtt ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc
     7261 ccccgaacaa cataatcaga tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg
     7321 gtttggtcag cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg
     7381 ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa
     7441 gaatggcgat gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga
     7501 gtagccccgc ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga
     7561 atagctattc gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg
     7621 gcatagagtg ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg
     7681 atccatactg cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc
     7741 agagacgcct ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata
     7801 ttttaccttt cgaaatttct gataatcgaa agttaaatta cggatcttca tcacataaaa
     7861 taattttttc gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta
     7921 tctgaatcga ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta
     7981 acgataataa aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc
     8041 tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg
     8101 gatcgatttc attccaggtg aaaatgccga aacgctgatt gaaaaataca acgctcagtt
     8161 ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc
     8221 gttcaatgct gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt
     8281 taacattcca atgctcgtgg aaacgttaat ggcccgtgat gatgacccga gctttgatga
     8341 actggttgcg ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc
     8401 ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc
     8461 aaaaccaatg gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct
     8521 gattcacggt caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt
     8581 tgttagtgat gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc
     8641 tccgggcgta acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc
     8701 gaaatatgct ggcgaacgcg taatgctgtt atttaccaac ccaacagatg tagagcgtct
     8761 cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg
     8821 taaaacccag gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa
     8881 actgaatgca cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa
     8941 aatgatggat ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcattcagtt
     9001 ttcacactta agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt
     9061 gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag
     9121 tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc
     9181 ggtattatta tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct
     9241 gcagttgcgc ctgacgccgc tctggcttct atcatttcta ccattctggt tatcgcaggt
     9301 catcagagca ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta
     9361 ctgaccatca tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct
     9421 gataacggca acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca
     9481 atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag
     9541 aacatgctga atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cgggggcatg
     9601 atcgtggtgg ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg
     9661 ttcttctacc tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt
     9721 gtgattggta ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta
     9781 gccggtgcgc ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag
     9841 gtgagcgaaa tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat
     9901 attcgtggcg tattcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg
     9961 caggcactgg gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac
    10021 aacgaagctc gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc
    10081 gtggctgcac cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca
    10141 gagatcgatg acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt
    10201 gtaggcgacc cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt
    10261 atcgcgatga gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg
    10321 cgtctggcaa cccgttacta cggcgtagct tatggttact ccaaaggtat cgatatcgtt
    10381 aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg
    10441 tttgtcatgg gggcattggt taacaagtgg acacatgtca acatcccgct ggtggtttcg
    10501 accatcaccg ggcaggatgg acagacccgt gtcaccaccg tgcaaactat tcttgaccag
    10561 ttaatgccag gccttgtacc actgctgctg acatttgctt gtatgtggct gctgcgcaaa
    10621 aaagttaacc cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct
    10681 tgcggcctgc tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt
    10741 ttatctggag gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc
    10801 ctggccttcg cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg
    10861 gcaattcctt tgctccgccg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc
    10921 attcttatct ataacaacgt cacgaatcat ggcgcgctaa taacaacatg gttattaagt
    10981 gcactggctc tgatgggttt ttatatattc tggatccgca ttccgaagat catctttaaa
    11041 caaaaaggtt ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac
    11101 ttgtcggaag atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt
    11161 cgaaatatcg acgatctgga aaaaatttat aagcttctcg
//
LOCUS       DAJTRQ010000124        10946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000124 DAJTRQ010000000
VERSION     DAJTRQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..419)
                     /locus_tag="OO332_003350"
     CDS             complement(<1..419)
                     /locus_tag="OO332_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5943713.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKAL"
     gene            548..883
                     /locus_tag="OO332_003351"
     CDS             548..883
                     /locus_tag="OO332_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5943714.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(880..1602)
                     /gene="folM"
                     /locus_tag="OO332_003352"
     CDS             complement(880..1602)
                     /gene="folM"
                     /locus_tag="OO332_003352"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5943715.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(1639..3021)
                     /locus_tag="OO332_003353"
     CDS             complement(1639..3021)
                     /locus_tag="OO332_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5943716.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(3207..4151)
                     /locus_tag="OO332_003354"
     CDS             complement(3207..4151)
                     /locus_tag="OO332_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5943717.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            4675..6207
                     /gene="pntA"
                     /locus_tag="OO332_003355"
     CDS             4675..6207
                     /gene="pntA"
                     /locus_tag="OO332_003355"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5943718.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6218..7606
                     /gene="pntB"
                     /locus_tag="OO332_003356"
     CDS             6218..7606
                     /gene="pntB"
                     /locus_tag="OO332_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5943719.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(7631..8665)
                     /gene="tqsA"
                     /locus_tag="OO332_003357"
     CDS             complement(7631..8665)
                     /gene="tqsA"
                     /locus_tag="OO332_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5943720.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            9076..9441
                     /gene="mdtJ"
                     /locus_tag="OO332_003358"
     CDS             9076..9441
                     /gene="mdtJ"
                     /locus_tag="OO332_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5943721.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            9428..9757
                     /gene="mdtI"
                     /locus_tag="OO332_003359"
     CDS             9428..9757
                     /gene="mdtI"
                     /locus_tag="OO332_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5943722.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9796..10617)
                     /locus_tag="OO332_003360"
     CDS             complement(9796..10617)
                     /locus_tag="OO332_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5943723.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10717..10800)
                     /locus_tag="OO332_003361"
     CDS             complement(10717..10800)
                     /locus_tag="OO332_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943724.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
BASE COUNT         2749 a         2656 c         2814 g         2727 t
ORIGIN      
        1 tgcagggctt tgtagggagt cagagacgtt tcctgaagac ctttggtcag tgtggcttgc
       61 tcattctgac gcaaatgcaa acgtaaacgc gctagcaaaa cagcaggggg cgtcgttttg
      121 agaatatagt cgcaggcacc catttccagt gccaggatgt ggttcatatc gctatcgaga
      181 gaggttagaa gaacaatcgg tccagaccac tttgcgcgta aatcacgaca aatggtcatg
      241 ccgtccttgc ctggtagcat gatgtcgagt aacaccaaat ccggattttc tcgcaaaatg
      301 gtttcttcgg cctggtcgcc gcgcggttct acggtaacct gcatatcatg ttttgccagg
      361 tacgcggcaa tcagtgaacc gacttccgca tcatcttcca caaatacgat agtgttcata
      421 acattcacgg tagatataaa aacgtcacaa tacaccgcgc tgtttttact tactatcaat
      481 cgtttctaaa caagttttca tcaggtattc tactgatgac ttgttatgga agtgttaagg
      541 taaaaagatg gggctggtaa tcaaagccgc gctcggtgcg ctggtagtgc tgttgattgg
      601 tgttttagca aaaacgaaaa attattatat cgccgggctg attccacttt tcccgatctt
      661 tgcgcttatc gcgcattata ttgttgccag cgaacgcggc attgaagcct tacgcgcaac
      721 catcattttt agtatgtggt cgattattcc ctattttgtt tacctggtgt cgctgtggta
      781 tttcaccgga atgatgcgct tgcccgccgc ttttgttggt tctgttgcgt gttgggggat
      841 aagcgcatgg gtgttgatta tatgctggat aaagctgcat taacgcagat gacgaccgcc
      901 atcaagtggg aaactgcgtc cggtgacaaa gcaactggta agtaagtaat cgaccaggtc
      961 gatcacttct ttctcgccag gcgcggtttt catcagtgat ttattcagcg cctgttgtcg
     1021 atattcggca tcatcatgtt cattaaacag gatcagcgat ggcgcaatag aattcacttt
     1081 cacttccggt gccagcttgc gggcaaacga gcgggtcata ttatccagtg ccgctttgct
     1141 tgcagcatac gcaacatgtt tgtcgctgcc gcgctccacc acataatcgg taaagtgaat
     1201 gatatcgctg gcggcgtgtc cgtgcccacg cagtaatctt tccagcgcat ggttgagcag
     1261 gtatggggta ttaacgtgga tctgcatcat gcaagccagt acgtcggcca gtggcgcacc
     1321 cggtttttcc gcaatccacg cactggcgtt atgcaaaata gcacgcagac catgggtggt
     1381 ttttagtact tcatcggcaa acgccatcac accgtcgttg gtcgaaaaat cagcctgaat
     1441 acactgcgca cctgcattaa tcagtccatc aatggctgga tagtgtgtcc gatagctgac
     1501 aatcaccggt tgcttttgat taatgaaatg ccatgcgagg gcgaggccga tgcgacgacc
     1561 tccgccagta attaatattg gcaagggctg ggttttaccc atcgttatct cctttgctat
     1621 ccaacgatgg gatgaacgtt atcccaccag catccaggtc gccggaactg aggcaattaa
     1681 cagcatgcca atcagcacca tttcctgacg gttcagtaca ttatcatgtg tatgcgtttt
     1741 gcgcgcatac aggaaaacca gcagccccgg tgcatacaga acaacggata acagcaagtg
     1801 cattggacca gaggcgtaca ataaccataa gccataaatg caggcaccga cacctaccgc
     1861 tttatgcagt ggacgggttg cgattttcag caagaacgcg ccgaccagga aatagggcac
     1921 cagaatcatt tctgaggcga tggtcagcaa cgtgttgtaa tcggaaccgg tcagccagat
     1981 gagtaccaga caaatttgca cgcagatgtt ggtcaaccac agtgaggcgg atggcgcagc
     2041 ttgtgcgtcc tggcgcgcaa aaatgcgtgg gaatgcttta tgagtggcag ccaggaacgg
     2101 aacttctgcc gccatgatgg tccagcttaa gtacgcgccg caaacggaaa cgatcaaacc
     2161 ggcagcgatg atgatttcgc cccatggtcc catcatttcc accatcagac cggccattga
     2221 cgggttacga atttcagcca gttcaggacg tgccaccaca cccagcgaaa gcagcgttac
     2281 cagcaagtaa acgcccagag cggagagaac cgccagcagt gtcgctttgc caacatcacg
     2341 tttattacgc gcacgcgcag aaacaaccac ggcaccttcc acaccaatga aaacccacag
     2401 ggtgatcagc atggtgtttt tcacctgttc ccaaacaggt acgccaagtg caagtccggt
     2461 gaagtcgagc ttgaaggtat ccagtttgaa catcatcatc gccagcacaa caaacagacc
     2521 cagcggcaac aattttgcca gtgtcgccac caggttaatg ctggcagcgg tttgcacccc
     2581 gcgcaggatc aaaaagtgaa caatccacaa taaagccgat gcaccgacta tcgattgcca
     2641 ggtattacca tcgccaaaca ggcgcaattc cggcgtgtcc gtaaaaaagc ttaacgcgga
     2701 aaaaacgatc accagatagg agacgttggc gatgaccgcg cacagccagt atccccatgc
     2761 ggaacaaaag ccgattagct cgccaaaccc ttcgcgggca taggtaaaga taccgccatc
     2821 aagttcggga cgaatgcgcg tgaggatcag catggcaaag gccagcaata aaatgccagc
     2881 gccagtaata ccccagccga tgagcagtgc tgccgggctg gcaactgccg ccatattttg
     2941 cggcagactg aaaacacccg cgcccagcat tgagcttaat accagcgcgg tgagtgcgct
     3001 cagtcccagt ttcttttcca tcgatatcca gtgattaaaa cgtgataaga agaataaata
     3061 ttagggcaaa acgcataaaa atggagtttg ccactgaagc gcgggatttt acgaaaagat
     3121 gtgtctgcat gcaatggtcg cggggaaatt tgtgactaaa aatgcgaaag gcggcatcaa
     3181 gccgccttat aggagtaact gaatagttat ttatacagat ctgcgctgac ggtcaggtta
     3241 ttaccacgtt tctgccactg gcgggtgatg tggtaatact tagcaccttt cttcgcggca
     3301 cgtttcgcaa cctgatagga gacttcggtc atgttgccgt agttgccaga gaatttgatg
     3361 ctgtcgaacg ggaccatcat cgctgctgtc gctttgttca gttcttcgac tttagtgcca
     3421 tccgggagcg tgacggtgta acgcccgcct tttgatgact gggtttcaaa gaagcgaccg
     3481 acttcagaac ttggtgatgc ggtagtcgca acacccggga tctcaacttt cttcgcggct
     3541 tcgccaccgg cagccagagc tgcacgtcct gcttcggaat ctgccgggat cacatccggg
     3601 ctctggacga tacgtttctt agcatctttt ttatagatga atgcagtaat acgctggttg
     3661 ccgccctggt tggcatcgat ttgacgaacg atgtagaaag agtaggcacc tttcgctttt
     3721 gccgctttgg tgatggcatc attgacttct ggctggctac gatagaagcc ctggacggtg
     3781 accgtgtcaa acggttcaat cagaacagcc tgatctttcg gcagttcgac aacaccgtta
     3841 attacgcgat tacttgtttc ttctgctttt tcagcatcgg ctttatagag gtcagcgacc
     3901 acacgccagt taccgctgtt accaaaatca gaagtgtcga caacataaaa agaggcggca
     3961 ccttctttat ctgcgcgacg agaaacggct ttcaccgctt cgccaatagc attaaaacga
     4021 ccggtaacca ctacacggtc aaaaggttta accgctgccg cttgctccgg tgtcagttct
     4081 gttgctgcgt taacggagaa tgccgtagca gaaagcagtg ccgacgccag gagggtgttc
     4141 ttaagcttca taaaaataat ccttcgcctt gcgcaaacca ggtactggta ttgttattaa
     4201 cgagaaacgt ggctgattat tgcatttaaa cggtgtaact gtctgcgtca tttttcatat
     4261 cacattcctt aaaccaattt taatcctgct caaatgaccg tctatgctta aaaaacagcc
     4321 gtatcagcat cattactact gaagcaactg aattgtataa gttaatttaa tgttaagtag
     4381 tgattcgtgc cggggcgatg tctcgtttta cccgaccgtc gaagacaatt atcagtcttt
     4441 atccggcgtt ctaaggtgtt tatcccacta tcacggctga atcgttaata ttttgcgagt
     4501 tcacgccgaa atactgattt ttggcgctag atcacaggca taattttcag tacgttatag
     4561 ggcgtttgtt actaatttat tttaacggag taacatttag ctcgtacatg agcggcttgt
     4621 gtggctcctg acacaggcaa accatcatca ataaaaccga tggaagggaa tatcatgcga
     4681 attggcatac caagagaacg gttaaccaat gaaacccgtg ttgcagcaac gccaaaaaca
     4741 gtggaacagc tgctgaaact gggttttacc gtcgcggtag agagcggcgc gggtcaactg
     4801 gcaagttttg acgataaagc gtttgtgcaa gcgggcgctg aaattgtaga agggaatagc
     4861 gtctggcagt cagagatcat tctgaaggtc aatgcgccgt tagatgatga aattgcgtta
     4921 ctgaatcccg ggacaacgct ggtgagtttt atctggcctg cgcagaatcc ggaattaatg
     4981 caaaaacttg cggaacgtaa cgtgaccgtg atggcgatgg actctgtgcc gcgaatctca
     5041 cgcgcacaat cgctggacgc actaagctcg atggcgaata tcgccggtta tcgcgccatt
     5101 gttgaagcgg cacatgaatt tgggcgcttc tttaccgggc aaatcactgc ggccgggaaa
     5161 gtgccaccgg caaaagtgat ggtgattggt gccggtgttg caggtttggc cgccattggc
     5221 gcagcaaaca gtctcggcgc gattgtgcgt gcattcgaca cccgcccgga agtgaaagaa
     5281 caagttcaaa gtatgggcgc ggaattcctc gagctggatt ttaacgagga agcgggcagc
     5341 ggcgatggct atgccaaagt gatgtcggac gcgttcatca aagcggaaat ggaactcttt
     5401 gccgcccagg caaaagaggt cgatatcatt gtcaccaccg cgcttattcc aggcaaacca
     5461 gcgccgaagc taattacccg tgaaatggtt gactccatga aggcgggcag tgtgattgtc
     5521 gacctggcag cccaaaacgg tggcaactgt gaatacatcg tgccgggtga aatcttcagt
     5581 acggaaaatg gtgtcaaagt gattggttat accgatcttc cgggccgtct gccgacgcaa
     5641 tcctcacagc tttacggtac taacctcgtt aatctgctga aactgttgtg caaagagaaa
     5701 gacggcaata tcactgttga ttttgatgat gtggtgattc gcggcgtgac cgtgatccgt
     5761 gcgggcgaaa ttacctggcc ggcaccgccg attcaggtat cagctcagcc gcaggcggca
     5821 caaaaagcgg caccggaagt gaaaactgag gaaaaatgtg cctgctcacc gtggcgtaaa
     5881 tacgcgttga tggcgctggc aatcatcctg ttcggttggc tgtcaagcgt tgcgccgaaa
     5941 gagtttcttg ggcacttcac cgttttcgcg ctggcctgcg ttgtcggtta ttacgtggtg
     6001 tggaatgtat cgcacgcgct gcatacaccg ttgatgtcgg ttaccaacgc gatttcaggg
     6061 attattgttg tcggagcact gttgcagatt ggccagggag gctgggttag cttccttagt
     6121 tttatcgcgg tgcttatagc cagcattaat attttcggtg gcttcaccgt gactcagcgc
     6181 atgctgaaaa tgttccgcaa aaattaaggg gtaacatatg tctggaggat tagttacagc
     6241 tgcatacatt gttgccgcga tcctgtttat cttcagtctg gccggtcttt cgaaacatga
     6301 aacgtctcgc cagggtaaca acttcggtat cgccgggatg gcgattgcgt taatcgcaac
     6361 catttttgga ccggatacgg gtaatgttgg ctggatcttg ctggcgatgg tcattggtgg
     6421 ggcaattggt atccggctgg cgaagaaagt tgaaatgacc gaaatgccag aactggtggc
     6481 gatcctgcac agcttcgtgg gtctggcggc agtgctggtt ggctttaaca gttatctgca
     6541 tcatgacgcg ggaatggcac cgattctggt caatattcac ctgacggaag tgttcctcgg
     6601 tatcttcatc ggggcggtaa cgttcacggg ctcggtggtg gcgttcggca aactgtgtgg
     6661 caagatttcg tctaaaccgt tgatgctgcc aaaccgtcac aaaatgaacc tggcggctct
     6721 ggtcgtttcc ttcttgctgc tgattgtatt tgttcgcacg gacagcgtcg gcctgcaagt
     6781 gctggcattg ctgataatga ccgccattgc gctggtattc ggctggcatt tagtcgcctc
     6841 catcggtggt gcagatatgc cggtggtggt gtcgatgctg aactcgtact ccggctgggc
     6901 ggctgcggct gcgggcttta tgctcagcaa cgacctgctg attgtgaccg gtgcgctggt
     6961 cggttcttcg ggggctatcc tttcttacat tatgtgtaag gcgatgaacc gttcctttat
     7021 cagcgttatt gcgggtggtt tcggcaccga cggctcttct actggcgatg atcaggaagt
     7081 gggtgagcac cgcgaaatca ccgcagaaga gacagcggaa ctgctgaaaa actcccattc
     7141 agtgatcatt actccggggt acggcatggc agtcgcgcag gcgcaatatc ctgtcgctga
     7201 aattaccgag aaactccgcg ctcgtagtat taatgtgcgt ttcggtatcc acccggttgc
     7261 ggggcgtttg cctggacata tgaacgtatt gctggctgaa gcaaaagtac cgtatgacat
     7321 cgtgctggaa atggacgaga tcaacgatga ctttgctgat accgataccg tactggtgat
     7381 tggtgctaac gatacggtta acccggcggc gcaggatgat ccgaagagtc cgattgctgg
     7441 tatgcctgtg ctggaagtgt ggaaagcgca gaacgtgatt gtctttaaac gttcgatgaa
     7501 cactggctat gctggtgtgc aaaacccgct gttcttcaag gaaaacaccc acatgctgtt
     7561 tggtgacgcc aaagccagcg tggatgcaat cctgaaagct ctgtaaccct gacggcctct
     7621 gcggaggccg tcactcttta ttgagatcgc ttaacagaac ggcgatgctt tgacctcccg
     7681 ctgtttgttc aagcgcaatt ttgacaataa ttgtcaacgg cacggaaagc agcataccca
     7741 ccggtcctaa caaccatccc caaaaaatca acgacaaaaa taccaccaat gtcgaaagcc
     7801 ccagcccacg tcccatgatg cgcggctcca gaatattgcc gaagaccaga ttaatcagca
     7861 gatatcccgc cagcaccagc aacgcttcgt agaagccatt aaacaccagt acctgagcga
     7921 taggggggat tgccgcgaga actgaaccaa tattagggat gtaattaagc gcaaaggcca
     7981 gcaatcccca gacaaaagcg aagtgaacat cgagtgcggc gagcatcgcc caggcgacca
     8041 agccggtgat gatgctgatg gctgttttca gcaccagata atgagaaaca ctgtcaatcg
     8101 cacgttgaat cgccgccatc ccttcaaccg gacgcgccat catttgctga aattttccgg
     8161 gcaattgtgg cacttcgagc agcataaaca gcaccgtcag cagcaataaa aatattgatg
     8221 acatggcatt agataactgc gtcaataagt tggtgagcaa cgtcatcgcc gcgttcggat
     8281 caatgtaatg cgccagttgg tcaactgaga cgtcaatccc tacgcgttgc aacaacggct
     8341 caagagcttg cagcggcgtc ataatagagt tgcgatattg cggtaacgtc cgcgtcaact
     8401 cgttgagcgc ggaacccaga taagctaata gcaacaccat cgccatcacg atgattgtca
     8461 tcaaaatcga cactgccagt acacgcggca cacgccagcg gaccatatgt tgcaccagcg
     8521 ggttaagaat aacagcaata aataatgcga gaataaacgg cacgatgatc tcggcggcaa
     8581 aacggatacc gcagagaata atgaccagca ttcccaacat aatgacgatt tttaggccat
     8641 tgagcgtgat gatcggcttt gccatgcttg tccccgtttt tcttcctttg ctaataataa
     8701 tgtctttttc gctccgtcat cataacgaaa atcaaaccgc tcaggtaaag aagtgaaaat
     8761 attttgagtt aattcttaag ctatgataca aatcaggcgt gttcaactac cgaggacaat
     8821 tatcatctgc gatgacgaga agcaacactg cggataattg taatattatg gacaatttgt
     8881 tcaggacgtt attttctaca tttacccatc ttcgtacctc atccactatc ttgctcgtag
     8941 gtgagcagca ctggcgcaac gcgctttagt cctttcttct gcctaagctt gccctatgcg
     9001 cagggcttga ccactttact ttggtttcgc tgaattaagc gaaaattaaa atagttctct
     9061 tgcaggagaa ggacaatgta tatttattgg attttattag gtctggctat tgctacagaa
     9121 attaccggta cgctgtcaat gaaatgggcg agcgtcagtg agggaaatgg cggctttatt
     9181 ttaatgctgg tgatgatttc tctgtcgtat atatttctct ctttcgccgt taaaaaaatc
     9241 gccttaggcg tagcttatgc gctgtgggaa ggtatcggta ttttatttat taccttgttt
     9301 agcgttttgt tattcgacga aagtttatcg ctgatgaaaa ttgccgggtt aaccaccctg
     9361 gtcgccggga tagtgttgat caaatcaggt acccgtaaag cgcgtaaacc tgaactggag
     9421 gtgaaccatg gcgcagtttg aatgggttca cgccgcctgg ctggcattgg caatcgtgct
     9481 ggaaatcgtt gctaacgtct ttttgaaatt ttctgacggc tttcgtcgca aaatatttgg
     9541 cttgctctcc ctggcggcgg tgctggcggc ctttagtgcg ctttctcagg ccgttaaagg
     9601 gatcgacttg tctgtcgctt atgcattgtg gggcgggttt ggtattgccg ccacgttagc
     9661 cgcaggttgg atcttgtttg gtcaacggtt aaatcgtaaa ggctggattg gcctggtctt
     9721 gctgttggct ggaatgatca tggtgaaact tgcctgatga agacgctgcc cgcgctgtcg
     9781 ggcagcgttt gaacattatt tttgcgacag ttgatccagc ttgtcgcgaa aaccggtaac
     9841 agaaatggcc cggttatcgg cgcgccagcg atctttcgcg gcaggagccg aactttgcac
     9901 cccaattaat tgccagccgt catcggtatg caacatcaga ggcgaaccgc tgtcacccgg
     9961 caaggtatcg cactgatgtg acatcaccga cgtttgcgcc cagccagtca cttcacagtt
    10021 ttgatgactg tataacgtat cgagatgatc ttcagggtat cccgcctgag tcactttacg
    10081 accagccgat tttaatgcgg cagtaagcgc ggctttatct ccctcaaata acggcaacgg
    10141 tgtaatgcca gaagggggat tacgtagcac aatcaatccg aagtcccacg gtgcagctgc
    10201 gggaggaaca atccaaccat ccccatctgc ttttaaccgc tttcccagtg tcggatcaac
    10261 gcggccttct atgtcgtgga tctcatagcg ccaaagacct ttatttgaca caaaacgcag
    10321 cgctaccgcc ttatccgctt tacccttagg cggcgtcaat aaacagtgtc ctgccgttaa
    10381 tgccagattg ggtgcaatca gcgtcgccgt acataaattg ccgctggccg tttccagttg
    10441 cccaaccgca tcccacggtg attgggtcgt gtcattcact ggcacacgat catcatgacc
    10501 aaaaaacagg gtgctgacct catcgtttgc cgatttggca acgtctggtt tatctgcaaa
    10561 cacaaaagca gacgtcaaac taattgcacc caacactaca gcaatggttg tacgcatatc
    10621 acactctggt gggtaattat gattattaaa agcaaaccct cataaatact atagacggga
    10681 cgggatgaaa gtgggagtaa aatcagataa ctacagtcag gaaagataaa aacgggcggc
    10741 aatcagtgcg cataaaataa ggatgataag aatgaactca aagcgataac ggcccaccat
    10801 actaccctcc gctaaaggcg gtgctaaacg ttacccgctt agcaccgtaa ttttcaggcg
    10861 cgagggggcg cgccagcatt actgttgaaa acttacgctg cgggttgtgc agcaggtttt
    10921 gccggttgct gatggccgtg tttccc
//
LOCUS       DAJTRQ010000125        10879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000125 DAJTRQ010000000
VERSION     DAJTRQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            129..1826
                     /gene="maeA"
                     /locus_tag="OO332_003362"
     CDS             129..1826
                     /gene="maeA"
                     /locus_tag="OO332_003362"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5943725.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1960..2970
                     /gene="adhP"
                     /locus_tag="OO332_003363"
     CDS             1960..2970
                     /gene="adhP"
                     /locus_tag="OO332_003363"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5943726.1"
                     /translation="MKTAVITKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3116..3400
                     /locus_tag="OO332_003364"
     CDS             3116..3400
                     /locus_tag="OO332_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5943727.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="OO332_003365"
                     /pseudo
     CDS             complement(3628..4281)
                     /gene="fdnI"
                     /locus_tag="OO332_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
     gene            complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="OO332_003366"
     CDS             complement(4274..5158)
                     /gene="fdnH"
                     /locus_tag="OO332_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5943728.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="OO332_003367"
     CDS             complement(5171..8218)
                     /gene="fdnG"
                     /locus_tag="OO332_003367"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7631..7633),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5943729.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8450..9331
                     /gene="yddG"
                     /locus_tag="OO332_003368"
     CDS             8450..9331
                     /gene="yddG"
                     /locus_tag="OO332_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5943730.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9590..10690
                     /gene="ompC"
                     /locus_tag="OO332_003369"
     CDS             9590..10690
                     /gene="ompC"
                     /locus_tag="OO332_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5943731.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2675 a         2786 c         2689 g         2729 t
ORIGIN      
        1 gacgattaaa caccattgcc tgcgcaatgg tgtttttatc tgctttatac ttggggacga
       61 cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt caaaaaagag
      121 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
      181 ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
      241 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
      301 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
      361 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
      421 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
      481 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
      541 ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac ggtgaacgta
      601 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
      661 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
      721 gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
      781 tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
      841 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
      901 accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact gcggcggtaa
      961 cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
     1021 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
     1081 cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg gtcgatcgct
     1141 ttggcctgct gacggacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
     1201 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg ctggatgtgg
     1261 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
     1321 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcta
     1381 acccgacgtc tcgcgtggaa gccacaccgc aggacattat cgcctggacc gaaggtaacg
     1441 cgctggtcgc cactggcagc ccgtttaatc ctgtggtatg gaaagataaa atctacccta
     1501 tcgcccagtg taacaacgcc tttattttcc cgggcatcgg cctgggtgtt attgcttccg
     1561 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacactt gctcagtatt
     1621 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
     1681 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtca
     1741 aaacgtctgc tgaagctttg caacaagcca ttgacgataa tttctggcaa gccgaatacc
     1801 gcgactaccg ccgtacctcc atctaagcct gcacccggta gtgaaggcta ccgggctatt
     1861 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
     1921 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaagactgc agttattacg
     1981 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
     2041 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
     2101 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
     2161 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
     2221 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
     2281 aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt caccgattac
     2341 gcggtaaaag tgccagatgg tctggactcg gcggcgccca gcagcattac ctgtgcgggg
     2401 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
     2461 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc
     2521 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatgggtgca
     2581 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
     2641 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
     2701 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
     2761 gatatcccac gtcttgtgct ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc
     2821 caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
     2881 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
     2941 ggccgtatgg tgattgattt ccgtcactaa gaggtctttg ctgcgactcc catgttcggg
     3001 tcgcagcatc gcacactctc caacatgaaa tggctaaatg gattattgtt gcgtaggaag
     3061 gaacaataat gatagtgttc ccagagaagt aacgctactg agagtaaaga cactaatgaa
     3121 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
     3181 cagcctgcgc gagtttgcca gagcaatgga aattgcgccc tcaacggcaa gccgattgct
     3241 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
     3301 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac
     3361 ggtggttgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cttataaagc
     3421 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
     3481 acgttcactt caatctattg tatttgaagg gttttcatgc cggataaggc attcacgacg
     3541 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatggcgcct tttacagcgc
     3601 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
     3661 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
     3721 cttcccttcg atcatccctt taatcgaccc tttcacccag aatgccatat acatatggat
     3781 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac
     3841 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
     3901 cagcacgaaa atcatgctca tgatcgacta gaacatcatc ttttgcccgg cgttgtactt
     3961 accaacatcc gccactttgt gctcattgcc ttttaatact tcgacaatgt tcaacagcca
     4021 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
     4081 tgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc
     4141 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
     4201 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
     4261 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
     4321 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
     4381 gcggtttcaa cgcgcctttc cacaggctta ctgaggtgtc gatcttcgga tctttcggca
     4441 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
     4501 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
     4561 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
     4621 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
     4681 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
     4741 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
     4801 gaatgattgc accagcagac gggcacgcct tcaggcagcc cggatcttca cagtgcatac
     4861 agccgtcttt acggatcagc cactccagct tgccattctg ttcggtttcg ctaaagcgca
     4921 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaacaccg acgcagtgcc
     4981 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
     5041 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
     5101 gcggcgtgat ggagttagtt gcggaccttt tgataatatc ctgcgtttcc atagccatct
     5161 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
     5221 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
     5281 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
     5341 gcggtttcag acggcgcgtt accactgcca ccgcgcggat aaagcctcgc ttgctggaga
     5401 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc ctgcgtttcg ctgatttcca
     5461 caaactgttc cggctgagca attgcgttga gcaacgcgtg cttggtccag gtgtggaagt
     5521 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
     5581 gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
     5641 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
     5701 tgttgatggc aaacaggcgt cccatccctt ccggctgcat aataaacggc ccggttggcg
     5761 tacccggtgc ggcattgccg aagtccggaa tatcgttacc cgtccacttg ctgccgttcc
     5821 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
     5881 tgtagagtac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
     5941 cggacgggtc ggagttatcg cggtttgcca tctggttacc ctgctctgtc cagctaccgg
     6001 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
     6061 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccaacgcat
     6121 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
     6181 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac aactcgcgca
     6241 gatgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
     6301 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcctcttcag
     6361 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
     6421 tcgactcgcc gtggttctgc cagaaggtag aggtttcagt caccagcgga tcgataacca
     6481 ccatgtactt cagtttgctc aggcagctca ccactttgtt tttatccggg aaggacgcaa
     6541 ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc atgttgaaat
     6601 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
     6661 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
     6721 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
     6781 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
     6841 gcagacctaa gtcagtcagc ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
     6901 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
     6961 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
     7021 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
     7081 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
     7141 cacagcgcgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
     7201 agttccagga cgatttgtcg tactggcgtt tttcagcgtc ataaccgctg aacaggccat
     7261 cttcgaaagc aaaatcatca cgcaccagta ggctggcgtt ggtgtaatgt ttaacgtatt
     7321 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
     7381 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
     7441 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
     7501 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
     7561 tatccaccca gtggttggtc atagcacctc gaccaaatgt tggagcaaga cttgctaccg
     7621 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
     7681 atttctgggt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
     7741 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
     7801 cagctttcat cagcttcgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
     7861 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcactg ttgacgtaat
     7921 ccagcaaacc ggcccctttc gggcacagcg caccacggct taccggatga tccgggtcac
     7981 cttcaatgtg ataaatcgct tctctggcgt tttttgcgcc atcacccagg ctatacatca
     8041 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
     8101 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
     8161 ttgttccagc catacctccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
     8221 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
     8281 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
     8341 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
     8401 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
     8461 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
     8521 tcgcggtgtc agcgaggggc tcggcccggt cggcggcgca gctgctatct attcattaag
     8581 cgggctgctg ttaatcttca cggttggatt tccgcgtatt cggcagattc cgaaaggcta
     8641 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
     8701 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tgtggcccag
     8761 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
     8821 aggattatta ttagccctgg tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca
     8881 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
     8941 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
     9001 cggaattacc gtttttgtcc tgctaacggg agcatgtctg tggatttact attttctcac
     9061 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
     9121 taccttagga tttgcttatg ctgcatggaa tgtcggtata ttgcatggca atgtcaccat
     9181 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
     9241 cagcgccccg ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct
     9301 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
     9361 taatcagata aattccgaca atattaaaaa ataaatttca aataccagta attcacctta
     9421 tatctcattt cgacttcgat cacatatcga taaataatat tatttcatct atttttctca
     9481 ttgtttttaa tcaccattct attttaaaaa cagtatgtca tttataaatg acgccgttta
     9541 aaattcgttt agaaaatacg tttaacgata attaaaagga ttctgttaaa tgaaattaaa
     9601 aatagttgcg gtggttgtaa ctggtttgtt agctgcgaac gtagcacacg ctgccgaagt
     9661 ctataacaag gatggtaata aactcgacct ttatggcaag gttaccgctc tacgttattt
     9721 tactgatgat aagcgtgacg atggtgataa aacttatgcc cgtctcggct ttaaaggaga
     9781 aacgcaaatc aatgatcaaa tgattggttt tggtcactgg gaatatgatt ttaaaggcta
     9841 taacgatgaa gccaacggct cgcgcggcaa caagacccgt ctggcctatg ctggtttaaa
     9901 aattagtgaa tttggctctc tggactatgg tcgtaactac ggtgtcggct acgacattgg
     9961 ttcatggacc gatatgttgc cagaatttgg tggcgatacc tggagtcaga aagatgtctt
    10021 catgacatac cgtaccaccg gtctagcaac ctatcgcaac tacgatttct ttggcttaat
    10081 tgaagggctg aactttgccg cgcaatatca aggcaaaaat gaacgtactg acaacagtca
    10141 tctttatggt gctgactaca cgcgtgccaa cggtgacggt ttcggtatct cctcaactta
    10201 tgtttatgat ggctttggta tcggtgcggt atataccaaa tccgatcgga caaatgcgca
    10261 ggaaagagcc gctgctaatc ctctcaatgc ctccggtaag aatgcagaac tatgggccac
    10321 aggtataaag tatgatgcca acaacatcta ctttgcagct aattacgctg aaacattaaa
    10381 catgaccacc tatggggatg gttatatctc taacaaagca caaagttttg aagtggtggc
    10441 gcaatatcaa ttcgacttcg gcttgcgccc atcactcgct tacctgaaat cgaaaggcag
    10501 agatctgggc cgctacgccg accaggacat gattgagtat atcgacgttg gtgcgacgta
    10561 tttcttcaac aaaaatatgt cgacctatgt tgattataaa atcaacctga ttgatgaaag
    10621 cgactttacc cgtgccgtag atattcgcac cgataacatc gtcgctacgg gcattaccta
    10681 tcagttctaa cgtcctctcc tgaccagccc ttctctgggc tggtttttga tttaagtgct
    10741 gctgtatgac tccttaagta aaaatgagca atttttgata tttctggtca ttcacctcaa
    10801 ttaatattat tccatatcct ccggggacat cttactataa tatttattga tgagtgatta
    10861 tgaccaatat taatacagc
//
LOCUS       DAJTRQ010000126        10569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000126 DAJTRQ010000000
VERSION     DAJTRQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..171
                     /locus_tag="OO332_003370"
     CDS             <1..171
                     /locus_tag="OO332_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5943732.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNL
                     SQSKNKESTNDV"
     gene            521..883
                     /locus_tag="OO332_003371"
     CDS             521..883
                     /locus_tag="OO332_003371"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR5943733.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            880..1797
                     /locus_tag="OO332_003372"
     CDS             880..1797
                     /locus_tag="OO332_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5943734.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1810..3075
                     /locus_tag="OO332_003373"
     CDS             1810..3075
                     /locus_tag="OO332_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5943735.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3142..3765)
                     /locus_tag="OO332_003374"
     CDS             complement(3142..3765)
                     /locus_tag="OO332_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5943736.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4516..4854)
                     /locus_tag="OO332_003375"
                     /pseudo
     CDS             complement(4516..4854)
                     /locus_tag="OO332_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4976..5236
                     /locus_tag="OO332_003376"
     CDS             4976..5236
                     /locus_tag="OO332_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR5943737.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5254..7989)
                     /locus_tag="OO332_003377"
                     /pseudo
     CDS             complement(5254..7989)
                     /locus_tag="OO332_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7990..9321)
                     /locus_tag="OO332_003378"
     CDS             complement(7990..9321)
                     /locus_tag="OO332_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5943738.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPNVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            complement(9331..10023)
                     /locus_tag="OO332_003379"
     CDS             complement(9331..10023)
                     /locus_tag="OO332_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5943739.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(10026..10481)
                     /locus_tag="OO332_003380"
     CDS             complement(10026..10481)
                     /locus_tag="OO332_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5943740.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(10481..>10569)
                     /locus_tag="OO332_003381"
     CDS             complement(10481..>10569)
                     /locus_tag="OO332_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943741.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
BASE COUNT         2705 a         2478 c         2303 g         3083 t
ORIGIN      
        1 tcagtgttac aggaaatggg aggctgggag tctatcgaaa tggttcgtcg atatgctcac
       61 cttgcaccta atcaccttac cgaacacgca cggcaaatag actcgatcct gaacccatcg
      121 gtcccaaatt tgtcccagtc aaaaaataag gaaagtacta atgatgtgta acttattgat
      181 ttaaatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa tctgtagcac
      241 caatcataac tatctgtttt agcaagcatt aaccgcattc actaagatat agttgatggc
      301 acaaacagaa agttgatgaa tgatgttgcc atatatatgt cacaaatacg gcacaacgat
      361 cttcaaacat atagccacac attcacagaa gagcacaaag ccttgcaatc cagtgcaaag
      421 atttgtgtgc cagagttttt ctcatcaact accgcaagta tcgatcgatt gagacttgga
      481 tgatagactt catgcctttg attattagct gatagaagaa atgttaaagc tatttgcaaa
      541 gtacacatct ataggcgtgc tgaacacact tatacactgg gtggtttttg gtgtatgtat
      601 ctatgccgcg cataccaatc aggctatggc aaacttcgca ggtttcgttg tggctgtgag
      661 ttttagcttc tttgcgaatg caaaattcac attcaaagca tcaactacaa cgatgcgcta
      721 catgttatat gtcgggttca tgggaacact gagtgctact gttggatggg ttgctgatag
      781 atgttcactt cctccaattg tcactcttgt taccttctcc gccatcagct tggtgtgcgg
      841 tttcgtctat tcaaagttca ttgtctttag ggatgcgaaa tgaagatatc tcttgtcgtt
      901 cctgtcttca atgaagaaga agcgatacca attttttata aaacggtacg tgaattcgaa
      961 gagttgaagc catatgaagt agaaattgtt ttcataaatg acggaagcaa agacgctacg
     1021 gagtcaatca ttaacgctct ggctgtttct gatccgctag ttgttccgct gtcatttaca
     1081 cgcaactttg gtaaagaacc agcattgttt gcagggttag accatacaac cggcgatgcc
     1141 gtgattccaa ttgatgttga cctgcaagac ccgattgagg ttattcctca tcttattgaa
     1201 aagtggcagg caggtgctga catggtgctt gctaaacgtt cagaccgctc aactgatgga
     1261 cgactgaaac gtaagacagc tgagtggttc tataaattac acaacaaaat aagcacccca
     1321 aaaatagagg aaaatgtcgg agattttcga ctcatgtctc gtgaggttgt ggagaacatt
     1381 aaactattgc ctgagcgcaa tcttttcatg aaaggcatac tgagctgggt tggtggtcag
     1441 acagatgtcg ttgaatatgt acgcacagag cgtgttgctg gcatctcaaa atttaatggc
     1501 tggaaattat ggaatctggc actggaaggt atcacaagtt tttcaacctt tcctcttcgc
     1561 gtatggactt atataggctt gtttgttgca agcatttcat ttttatatgg tgcatggatg
     1621 attatagaca ccattgtctt tggtaaccca gtacgcgggt atccctccat gcttgtatca
     1681 atacttttcc tgggtggagt gcaactgatc gggattggtg ttctcggtga atatataggt
     1741 agaatttatt tagaaacaaa atcaagacca cggtacttaa ttaaaagcag aaaataacag
     1801 gatgatctta tgaagatatt cataatgcgc aattggcaca agatatcaat ttttatactt
     1861 gcatttactt tgatttggct taggcgcatt gacattttga caaatgccca gttttgggct
     1921 gaggatgcag ttttttggta taaagatgca tacgaacaag gttttttcag ttcactcata
     1981 accccaagaa atggttattt tcaaacagta tcaacactca ttgttggtgc gacgacattt
     2041 attaatccaa tttacgcacc attattatca aacttttttg gaataataat tagagctata
     2101 attatatggt tcttatttac cgatagattt aaattcctaa gcaccacatc aaaaatcttc
     2161 atctctgtat atttaatttg catgcccggg cttgatgaag tccaagccaa tataacaaat
     2221 gctcactggt atttatcatt gtatgttgca atgataataa tatctgaaga atccaagtca
     2281 aaattatgga aagcacatga tttatttttt atagtattat ctggattgag cggtccattt
     2341 attatattca taattgcatc atctgttttt aaatatttac acttatcaaa aggagtaata
     2401 agcataagag gtttatactt attttatact cgactgccat atttggcaat gatcttgtgt
     2461 gggctgatac aggcaacatc aataattctt acatttaatg gaacaagatc acatgctcca
     2521 ttaggattca gttttgatgt tatgtcatct ataatatcat ctaatgtatt tcttttttca
     2581 tttgtccctt ggaatatcgc taatgctgga tgggataatc accttttatc gtacacacta
     2641 tcaactttag taattacatt atgtgttttc atttatatta aaggaaattg gcaaatgaaa
     2701 gtgtttgcca cccttccaat acttattgtc gcattttcta tggcaaaacc tcaactggca
     2761 gattctgttc ctcaactgcc aacacttgca accggaaacg ggtcaaggta ttttgtaaat
     2821 attcatattg caattttttc attaatatgc gtttgtttgt ttcaatgcat aaagaataaa
     2881 accttaaaaa tattctttaa aatctatgtt tcagtcatcc ttattgcaat gataaaatta
     2941 aactttttta taacccctct tcctgacatg aactggtctc aaggagcaga attaataaat
     3001 aaagcaaaac atggagaagc cgtctcattt acagtgctcc ctccatggtt aactcttgat
     3061 ttaataaaaa aataaaactt cacattaata aataacaatg aatccgcctc cctacgtatt
     3121 cccgtaggga ggctgtcgtg tttatagtag cctggtaatg ctaaatgcaa acgaactgga
     3181 gattcctgct ccgctaagcg tattgcgcag ggtcacccta aatgtatcta caccaatata
     3241 agtaacatca caactaactg aatcaggcgc agatataggt gttatctgaa ccacataacc
     3301 attagtagct gctgcgagcg gatgagaaaa cgttacatca tacacaccag tagcatgcgc
     3361 gttaacggat gaaacacccg gcgcaatgtc tacactacca ttggcgttaa ttctccctga
     3421 gcgcgtaact gactctgcag aaaccgatgt ggccttactg gttatttcca taaaggtgca
     3481 accaatatcg atcaattcgc aattgttccc tgttatccag tatggtcgat ctgcacttgg
     3541 ctgataatcc cctgcgctct ggaacgttgt gccattgagt aagactataa cttttccccc
     3601 acctgagctt gttacttgta tattcgtgtg cgtgtagcgt tcactgctca cgcgatgaaa
     3661 atttccaccg tttataacta ccgttacagg tcgcgtccca gtattatcta tagcaaggtc
     3721 tgcccccccg gcattagcct cgaagtatgg gttgtttaac accaaagcac acgaaccgtt
     3781 aaggccattt atggctaatt gcgcaccacc tgtaccggaa tccccctgag taccatttcc
     3841 ttcacaagtc aggctgttga tagtagttcc tgccccccat ctatcaccaa gtatacccca
     3901 tcgtgtattc tggcttgcag ttaatcggtt aactgtaatg gcattaagct ctgacccttc
     3961 actggagtta agtgcgcgcc agccaatacg gttaccctgt aagtacacat tatctaactc
     4021 tgacgttaac accgagtctg tacgcaaccc ttcgccggta gtgtgcccat aagacacaat
     4081 gttctctaat ttagtgtatg ctttattggt tacgagaaga ccgtaagacg atcccgaacc
     4141 tttaagtgta atgtccccca gataatcata ggcgtgtaca cgttgggttg ccccgactgt
     4201 atcaccgagc atctttaagt tgaaatccga ggcatggcag ttaaatatcg tttctgccat
     4261 attttctccg gaataatgag cacggactcc tgtcgaacca ataaatttag cgtcatagtt
     4321 gccgtaatca agaacgagtg gtgacgcgat atcgtacacc ccttgcttta tttttaatgt
     4381 tccaccgaaa ggccacgatg taagcgggtc ttgatatatt tcatttttta ttgcaaatag
     4441 ctcctcttgg agatttttgc cgctattagt gccaatgtga gcagctcctt catctgattg
     4501 aagtaactgc tttaactgat caggatcgta cttcagcaca ttctgaaaat agaactgctg
     4561 cgaaccatac gcatcatata cagccattga atggccttgc tcagttacaa atttggcaat
     4621 ctgcccgttg tacacaggat atccggcagc attgataaca attggctgag aaacagggac
     4681 gtgagatccg tcttcattct ctacataaac ctgaatctgg ttttcaggat ttaccgggtc
     4741 agtgtcaatt tttccgatat aaattttgcc attggctacg gctttaaaag aacgcgccat
     4801 agtgaagagt tgcgaaggca tgctgaccac aacatttgcg gcgatatctg acatttcatt
     4861 gctccagacg aatgatatga tgcaatcatg atgtgattgc ataccgaaat ggcactattg
     4921 aatatttatc cagtaggtta cgatgccatt ccacccaact ggtgaggcat caaggatgta
     4981 cagcaaatac gacgaggcac agtttcactt gagacttccg catgaactcc acgcgaaaat
     5041 taaacagtgt gcgaagatga ataacagttc gctgaactca gagataattg cagcgattga
     5101 agaatcattg gctaaacaaa gctctgcatc agtttacatt gacgatgcag agcgtatggc
     5161 agaacaacaa tctgatatgg ttaagaaaat tgtctttgat acgctcaagg agctatataa
     5221 aaaagacagc aactaaccat ccgtggcttt ccgttacttc tggttatcat cactgttcat
     5281 ccagtacatg atgcctagcc ttgatatggt tttcttatct gattctggaa gtgagttgta
     5341 aaacgagcgc caagcagaag agcgtcttac agttgtatca acgatgcttt ctggtgcttg
     5401 ttttactgcc agccttgctg ctttctggaa ttctggtgag ctaattagct tttctgcaga
     5461 ttcaattgat tcagcgccgc ccatcgccct gattttgctg gcgacttttg ccccaatttc
     5521 tgacccagca acagcaccta ctgcactgaa cggtcctgac acagttgaac caaccgcagt
     5581 gccaatgcgt tgggcatggt tagcaacaaa ttcatttgcc gcagtgacgc gattaaaccg
     5641 tttgacgaac tcgtttagtc ttcctgtagt tatctcgtaa gattttgcat ctttgatagc
     5701 ctttgcgacc ttatatacat cgttcagacc tgacatagtc tcttttggta aatgtctggc
     5761 aagattgcgc atctgcccgt ttgctaacat gttttgatac cagtcagcaa accctgcagg
     5821 attaaaatca gcgccacgtt ttccgttcga aagcatatcc cgaagacctg ttcccagtag
     5881 ctcggttctt agcttcctgg acggcgtgtt ttgcatcaat tcacgaaatc ctttggcatc
     5941 gcctttcgac attgcctgta gtgctgtagt tgctttgcga gaaacatcac cgttcagcgt
     6001 tctgccagtt agattaatca tttgctcttc catgcttttt cgcatctgaa caagcctctg
     6061 agcaacttca aaatcacgta atgcatttgt ctctgacagc gccgcctttt gatcatcggc
     6121 gagcgaaccg taaagcctcg ataaagcagc gcgatcagca tctttatatg gtccagaatt
     6181 cttatgaagt gcatcaccaa caagtcgtcg ttgcttattg agatttgcat aagttaatgc
     6241 gccgttcttg cctggattaa ctgcccgaaa gactctcttt tcaatagggt caaggttttt
     6301 catcccgcca agatcttctg ccacctgttt gagcattgac cttgttgatg gcgcatcaat
     6361 ttttgcacct gcaggcatcg ccttttcaac attgcgataa agctggtcgc ttcgtttcat
     6421 cagcgcagac attcttccat tgaccgtatc aataaacttg tcgctcatac caagagcatc
     6481 ttttgcaccg gaaacgcgat caattatctc acccgcgccc tgtgctagct gcctgattgc
     6541 ttcattttcc tgcacctgta gagcagaacc cgcacgggac ttgattgctt gctcaactgc
     6601 cttgtattgc tcgttcccgg aaaagtgtga agggagtaat gaatcaacat taagcctttc
     6661 agcagactcc agaacttcag cctgaggatt gatatctagt tcatcaagcg atgaagcgag
     6721 atttggcctt ttttgtgctg ctactgtgcg aacgacttcc tctgggttct tcgtcgcagg
     6781 cgtcattggt acaccttgtg ctactcttgc ctctgcggca tgaaccgcag gatttgttgc
     6841 tgcagcggca tcaccggaga atggtgatgt ttctgttggc gcagtggtcg ctctctgtgc
     6901 agatgtttta ttgccactaa ttatatttga tgcaccacga acaccacggg taacaccatt
     6961 tatcaggcca tgcgtagcaa tgctaagtcc ggcattaata gcggcatttt cggcaaaatc
     7021 accccgttga ttcgccgcgt cagcgagaga accaatgacc atgtttccag ctacacctgc
     7081 tccaggaaca agataaccgc ctattgactc tccagcttgc gcgtaggggt ctgtcggtct
     7141 gtctactgga cgataaacat catccaatac ttttggcccc ccaagcccct gactgattgc
     7201 attaatcaga cttgcgccac cctgcaatac gtcaaatggt atgtttacca gaccacgacc
     7261 agcctgttct gcaatttacc ctgcactttg accaccagtg agccaatcgc cagcttgttg
     7321 catcaatgat ggttcttccc gtgttggtgc attattggcc tgattaactg tttgttgctg
     7381 aacaggtttc ggtgcgacag aacttacggg ttgaggtggc tgctgactgg ctgcctgctg
     7441 ctcaatctga gcaaatggat tatttgggtc tgactgcaca cctgatgccg atacttgttc
     7501 ggatgactgt acttccagtt gtgcaaacgg gttgttaggg tcttgctgag gatgtacctt
     7561 tgcagaggtg gcgcgctgtt cgactgttga gtctgtcacc gggtcaccag cccattgagc
     7621 aaagcgatca tcaacgtaac cgcggccttc aggtcctggc gtatattcac cacgctttgc
     7681 cttcataacg ttgccgggac cgtcgtgata agcctgaaga gcgtcacgcc agttaccaaa
     7741 ctgctggtat atctttgcca gatagcgcgc gccagcgtca gcctgatatt cggggttttg
     7801 catttgctca tcggtataac ccatatcacg ccatgtccca ggcatgacct gagtcaatcc
     7861 tacagccccg gcggagctta ctgcggcagg gttgtaagaa gactccttgg cacccagtgc
     7921 agtcatcaac ccttctggca caccgtaacg tgcgccagcc tgctctaaca aatcacggta
     7981 attagccatt tactgcccca aagatggaag atatccgtag cgattaatga agtcgattga
     8041 cagctcgggg tgctgcttca ggtaatctat agaagcctga ggcgcttcca ctcgcttgat
     8101 accgttttgc tgaacgtact taccaaccgc ctcattacgc ttctggttga gcgtgttcag
     8161 gatgacgcca gcgttgcgac gaaaggactc ctcgctctgc gagttctgca gcgaaccaac
     8221 agcctggtcg agctttttgc cctcggcatc agaaagtgcg cccatgcctc gcatggcctg
     8281 aaccgctgtc aggtatgctt gggatttaaa ggtatcaagt cgtgcctgag tgtctgcagc
     8341 ctgtgagcct ggaacgttgg ggattactcc acgtaagcct gtaatgctct taagtgaagg
     8401 agaactaacg atatcgttca gagttaacat gctggttgtg agggtgttga tgccgtcttt
     8461 gtagccatca tttagcgctt gctgcttctg ctgcaactgc tggttgttgg ctgctatgcg
     8521 gctctgtatt tcctggcgct tcaggtcgtt agtttctgct gatagcatcc ggtcaaggcg
     8581 cttattttcg ttgttaatgc ggtttgtttc tgcgtccaga ttaatgcgct gctgacctaa
     8641 attcgcctgg atatcttgcc cgcgcattgt gattgcctga ttccgagcgg cggtttgcga
     8701 atccaggtcc tgaccgcgca tggtaacctg gcgaccctgc attttatcct gtaggtcaaa
     8761 gtatttttcg ggtccgagac tgttcatccc caggtgatcg acaaattcgc cgaactgccg
     8821 cgggttctgt tggtacatct gagcgacgtc ctgaggatta acgccaacac gagctaactc
     8881 accggcgttg ttttgcagcc atgattgcat tgcttctgga gacgatgacg caaggcgtgc
     8941 gccagccgct aaggtgccga tagaattacg ctggtcttca tcaatgaatc ccatgccttt
     9001 acgaacggat tcaatctggt ctggatattg agtagccaac tgacgcaaag caccgcgatc
     9061 accagacgca taagcattag cgtacgcctg ctgaaattct ttctgccgct gagcctgctt
     9121 ttcctgctga aacacccccg caatacctga aaggccttgc aaagcagtca gcccaacatt
     9181 gttagcgcct gaacgctcaa tatcattgtt ctgcctgata agctgaagcg tattgccgat
     9241 gtcatttacg ttcggagcgt ttgagttgac gccgccgata ccagccaata atccgccatt
     9301 tgatccttgc caagtagcca tgattacccc ttaaaacaac gagccaagca atccgatacc
     9361 agcaccaatg ccagcgcccc aaggtgttga tgttcccaaa aggctggcaa gacctgcacc
     9421 ggcaatcgca ccagacgttc cgccactaat tgctgtctga agacttgatg gtttgttggc
     9481 attagcagcg gcaagagctg cgctttgctg tgcaatgctg ctcatgttgt tggcgtatgt
     9541 ctgcccggcg tttgcctgac cttgcagcgc accaagccca acgtttgcca gattgttgta
     9601 attgctcatc tggtttgata accaagactg accgagtgtc ggcgcgatcg tagccagttg
     9661 attgcttgtg gctgtcgaac caagtccacc cgtcgcctcc gcagcagcaa gactctggta
     9721 acgagcctga cctgcaaggt ctttatactg ctgagagttg taatactgat taagtgcctg
     9781 cccctgacct tctaaactgg aaaggttctg aagctggtta acatactgct ccgcaagagg
     9841 cgtgaacgga gcaaggtttt tcatgatcgt ctgccactgc tgattttgca ggtctgcggc
     9901 atacttctga gcttctgctg catactttgc gcttttatca gagctgccac ctttcccgcc
     9961 tttttcaggg caataaggtt cctcgccgcg cagttttctg cccagcttaa atgcatataa
    10021 catggctatc tcccgtgatt caggaagtcg attagttctt cgcgtgtggc gctgtaaaac
    10081 gtcacgtcat ccacgccttt gaagtatttc ttgatggttc ctacacgctt aaggccaatc
    10141 attgcgcagt acatctgacc gtggcggaat ttgcgcgcag cgaacgatgt gacgcactga
    10201 acggtggtgt tagtcagaat gtatcgccag aacgccagcc cgatttcctt gctgaatcca
    10261 cgaatctctg gcaggtacat ggcgtggcaa tcaaaggtta gcggctgaat ctcctgatag
    10321 taaacaattc cgccgaactg cccgtgcacg ttaacctcaa agtaacggca ttcaggcttg
    10381 tagtcgtatc catcgccgtt gttgctcccg gcgataatgt cagtgtgatt tcctactgct
    10441 tcgatcaggt cgatgtttcg cgttggtttg aactgaatca tcactgctcc gcgattatct
    10501 tgatggttgt ggcagtaaac gccgcaccat ttgactgaat agttaacgta ctgccatttg
    10561 aggcaagaa
//
LOCUS       DAJTRQ010000127        10525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000127 DAJTRQ010000000
VERSION     DAJTRQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            87..1172
                     /locus_tag="OO332_003382"
     CDS             87..1172
                     /locus_tag="OO332_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5943742.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1243..1851
                     /locus_tag="OO332_003383"
     CDS             1243..1851
                     /locus_tag="OO332_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5943743.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1949..3340
                     /gene="selA"
                     /locus_tag="OO332_003384"
     CDS             1949..3340
                     /gene="selA"
                     /locus_tag="OO332_003384"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5943744.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3337..5181
                     /gene="selB"
                     /locus_tag="OO332_003385"
     CDS             3337..5181
                     /gene="selB"
                     /locus_tag="OO332_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5943745.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5371..6522
                     /gene="yiaY"
                     /locus_tag="OO332_003386"
     CDS             5371..6522
                     /gene="yiaY"
                     /locus_tag="OO332_003386"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5943746.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6687..8225
                     /gene="aldB"
                     /locus_tag="OO332_003387"
                     /pseudo
     CDS             6687..8225
                     /gene="aldB"
                     /locus_tag="OO332_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
     gene            complement(8444..8599)
                     /locus_tag="OO332_003388"
     CDS             complement(8444..8599)
                     /locus_tag="OO332_003388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943747.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8770..9093
                     /locus_tag="OO332_003389"
     CDS             8770..9093
                     /locus_tag="OO332_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5943748.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9099..10235
                     /locus_tag="OO332_003390"
     CDS             9099..10235
                     /locus_tag="OO332_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5943749.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10232..>10525)
                     /locus_tag="OO332_003391"
     CDS             complement(10232..>10525)
                     /locus_tag="OO332_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5943750.1"
                     /translation="GNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQH
                     PYEWWLDLVAIFRKREYLPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2498 a         2589 c         2909 g         2529 t
ORIGIN      
        1 gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac
       61 tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc
      121 tggcgacggc ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact
      181 acaaccaccc ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac
      241 aggtgacggg aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg
      301 aggtgctttt taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg
      361 acctgatgac ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg
      421 ccaacaccac ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttacc
      481 tgaaaggcag ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc
      541 aaaatttcct cgcgcaggat gcgttagtga aaggctcggt ggcggagcag gcgcagatcc
      601 agagtcagct cgacagtatg gtcaacggcg agcaatcgca gattgtgagc ttaagagcgc
      661 aacttactga agcaaaatat aatcttgagc agactgtcat tcgcgcacca agcaatggct
      721 acgtcactca ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg
      781 tgatggtctt catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc
      841 tgttacgtct gaaacctggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg
      901 tgttccacgg caaattgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc
      961 agggggtatt gcaatcatta acggtcgttc ccggcacgga cggtgtgctg ggaaccattg
     1021 aactggaccc taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg
     1081 tttactccga ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca
     1141 gttggatgca ttatctttat ttggatcatt gagacttata tcacctgtgt tttgcgtgag
     1201 cctgagccat actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc
     1261 tacaccagcc cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc
     1321 gaatttatta atgaactgcc ctataacgcg gataacggcg tggcgcaatt taacccgtta
     1381 gggaaagtgc cggtgctggt gaccgaagag ggcgaatgct ggtttgattc gccgatcatc
     1441 gctgaatata ttgagttaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag
     1501 tcgttgcggg tgcgcaaaat tgaggcgctg gcggatggca ttatggatgc cgggctggta
     1561 tcggtgcgtg aacaggcgcg tccggcggcg cagcagtctg aagatgaatt gttacgccag
     1621 cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc
     1681 aaaaccgata cggtcaatct ggcgactatc gccattgcct gtgctgtcgg atatctcaat
     1741 ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa
     1801 aacctgttta gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata
     1861 tgtcctcctg acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt
     1921 gagagggcct tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca
     1981 acttccggct attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta
     2041 tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat
     2101 tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg
     2161 gttgacgaaa gaggcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct
     2221 gcataccaac cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc
     2281 tatgcgttcg ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga
     2341 tcgggcgctg gcgcagctgc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa
     2401 taacaatgcg gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt
     2461 ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg
     2521 tcaggcaggc tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta
     2581 tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag
     2641 cattcagggg ttcaccaaag cgatagacga agctgaactg gtggcgctcg gcaaagagct
     2701 ggatgttcca gtggtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg
     2761 tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagctt
     2821 ctccggcgac aagttgttag gcgggccgca ggcagggatt atcgttggta aaaaagagat
     2881 gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct
     2941 cgcggcgctg gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt
     3001 accgaccctg cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt
     3061 acaggctccc cttgtcacac attacggcgc ggagtttgcg gtacaggtta tgccatgtct
     3121 ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt
     3181 tacaccccat gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt
     3241 gccagtgccg gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct
     3301 tgaagatgag caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac
     3361 gttgaccacg gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg
     3421 ccggaagaaa aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg
     3481 gatggtcgcg tgcctggttt tatcgacgtt cccggtcatg aaaagtttct ttccaacatg
     3541 ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg
     3601 atggcacaga cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca
     3661 gtggcgctga ccaaagccga tcgtgtggac gaagcgcgtg ttgatgaggt tgaacgccag
     3721 gtaaaggagg ttctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca
     3781 accgaaggtc ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag
     3841 cacgccagcc aacatagttt ccgcctcgcg attgaccgcg catttaccgt aaaaggtgcc
     3901 gggctggtcg tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg
     3961 ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca
     4021 gaaaccgcca atgccgggca gcgtatcgcg cttgacatcg cgggtgatgc ggaaaaagag
     4081 cagattaacc gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg
     4141 attgtcgagc ttcaaaccca tgcaccgctg acccagtggc agccgctgca tattcaccac
     4201 gccgccagcc acgtcacggg acgcgtttca ctgctggaag ataaccttgc tgaactggtc
     4261 ttcgacaccc cgttatggct ggcagataac gaccgcctgg tattgcgcga tatctctgcc
     4321 cgcaacacgc tggccggagc gcgcgtcgtg atgcttaacc cgccgcgtcg cggtaaacgt
     4381 aaaccagagt atctgcaatg gctggcttcc ctcgctaggg cacagaatga tagcgaagcg
     4441 ttagccgttc acctgcaacg cggcgcggtt aatcttcctg atttcgcctg ggcgcgccag
     4501 ctcaacggcg aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat
     4561 agcttgttga atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg
     4621 acttatcatg agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg
     4681 gcgttgccaa tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc
     4741 ggcgacatcc tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc
     4801 gaagagcagc aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg
     4861 gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc
     4921 caggcggcgc agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat
     4981 cggattgtcg aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
     5041 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
     5101 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gcgcgacgca
     5161 ttattatttc cggaaagata aggaaatgat taattaattt ttaaaataaa ttaatacaaa
     5221 tttcttatga ttttaaataa agcacattgt ttaatgaata caatgtgctt tttattagat
     5281 taattttgat aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc
     5341 cacccttttt tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct
     5401 gtgaatgtca tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga
     5461 tttacccgta ccttaattgt cactgacaat atgttaacga aattaggtat ggcgggtgat
     5521 gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct
     5581 aaccccacca cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat
     5641 agcgtgatct ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg
     5701 gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag
     5761 ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc
     5821 atcatcactg acgaagcgcg tcataacaaa atggcgattg ttgataagca cgtgactccg
     5881 ctgctttctg tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca
     5941 acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg
     6001 atcactgacg cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc
     6061 gttgaagatg gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttccttgcc
     6121 ggtatggcgt tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc
     6181 ggtttctaca acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta
     6241 ttcaacagca aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac
     6301 gtgacaggta aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg
     6361 gcgaagaaag tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc
     6421 gcggttctgg cgactaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca
     6481 actcacgaag aaattgtggt gatttatcgc gccgcaatgt aatcatcatt ccaacaacgg
     6541 ctggcaaatt gtcagccgct ttttcaacta tctctgtaat ccttgcccgt aaattcgtga
     6601 tagctgtcgt aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt
     6661 atacctcaca ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc
     6721 ccggcgagta tggtttcccc atcaagttaa aagcccgcta cgacaacttt attggcggtg
     6781 aatgggtagc ccctgccgat ggtgagtatt accagaacct gacgccggtg accgggcagc
     6841 tgctgtgcga agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc
     6901 acaaagtgaa agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta
     6961 agattgccga tcgaatggaa caaaacctcg agctgttagc gacagctgaa acctgggata
     7021 acggcaaacc cattcgcgaa accagtgctg cggatgtacc gctggcgatt gaccatttcc
     7081 gctatttcgc ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa
     7141 ccgtggccta tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact
     7201 tcccgctgct gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg
     7261 tgctgaaacc cgcacgtctt accccgcttt ctgtactgct gctaatggaa attgtcggtg
     7321 atttactgcc gccgggcgtg gtgaacgtgg tcaatggcgc aggtggggaa attggcgaat
     7381 atctggcgac ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc
     7441 aacaaattat gcaatacgca acgcaaaaca ttattccggt gacgctggag ttgggcggta
     7501 agtcgccaaa tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag
     7561 cgctggaagg ctttgcactg tttgccttta accagggcga agtttgcacc tgtccgagtc
     7621 gtgccttagt gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg
     7681 aaagcattcg cagcggtaac ccgctcgaca gcgtgacgca aatgggcgcg caggtttctc
     7741 acgggcaact ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgctgacg
     7801 tgctcacagg cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg
     7861 aaccgacgat tctgtttggt cagaacaata tgcgggtgtt ctaggaggag atttttggcc
     7921 cggtgctggc ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata
     7981 cgcaatatgg cttgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg
     8041 ggcgcggcat acaggctggg cgcgtgtgga ccaactgtta tcacgcttac ccggcacatg
     8101 ctgcgtttgg tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg
     8161 agcattacca gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggccgt
     8221 tctgatataa gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct
     8281 tatcatacct acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat
     8341 aaacaaagcg cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact
     8401 acgccaacag aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct cgcgtttcga
     8461 tggtgagcgg gcgaaccagg ccgtaaatca ccattggtga tccgaggcca atcagcaata
     8521 aacggtagat aattgaaggt ttaatacgct taatctgtac tgtggatgtt ttactggagt
     8581 tattcattag tttggccaat agttccacta tttcaatatg ataaacctcg tcctgttgaa
     8641 aaaccagaca gcacatttca gaaagtatat aaaaaacaat atgtcgttat ataaataagg
     8701 aaattgcact gcatcatggc atttcataat atgcgttaat tgttcttttt tatattcttt
     8761 gagggaaata tgttcctgga ctattttgca ctgggagtgc ttatttttgt atttctggtg
     8821 atattctatg ggattattat tttacatgat attccctacc tgatttctaa aaaacgtaat
     8881 catcctcatg ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt
     8941 atctggccat ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg
     9001 caaatccatg actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag
     9061 ctcgccgaca tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga
     9121 cctatgtggc ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga
     9181 ccatttccac agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga
     9241 actataacca tccgtatacc ttcaaggcgc aaaaagcggt tatttctatt cctgttgtcc
     9301 cacaggtgac aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag
     9361 gtgaggtgct atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg
     9421 cggatatcgt taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg
     9481 cggcgaatac tcagcaggca aaggccacgc gggataaatt cgctaaagag tatcagcgtt
     9541 acgcacgcgg cagccaggcg aaagtaaacc cgttttcaga acgcgatatt gatgtggcgc
     9601 ggcaaaatta tctggcgcag gaagcctccg taaagtcatc ggcggcggaa caaaaacaga
     9661 tccagagcca gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag
     9721 cgcagctcgc ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg
     9781 gttatgtgac ccaggtgctg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc
     9841 cggtgatggt gtttataccc gatcagaaac gacaaattgt ggcgcagttc cgtcagaact
     9901 ccttgctgcg actggcccct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta
     9961 aggtattcag cggcaagctg gcagccatta gtccagctgt tcccggcgga gcttatcagt
    10021 cgaccggcac cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca
    10081 ttgaactgga tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg
    10141 cggtctactc tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga
    10201 ccagctgggt gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag
    10261 gccgcagttg ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca
    10321 catactggac ccgccagggc aggtattccc gtttacggaa gatagccacc agatccagcc
    10381 accattcata cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga tcgctctgca
    10441 cacttttgtc tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgccgatt
    10501 gcgtgttatc gcaaacgtaa tttcc
//
LOCUS       DAJTRQ010000128        10466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000128 DAJTRQ010000000
VERSION     DAJTRQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..1280
                     /locus_tag="OO332_003392"
                     /pseudo
     CDS             1..1280
                     /locus_tag="OO332_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
     gene            1332..2487
                     /locus_tag="OO332_003393"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="OO332_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="OO332_003394"
     CDS             complement(2453..2575)
                     /locus_tag="OO332_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943751.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..4463
                     /locus_tag="OO332_003395"
                     /pseudo
     CDS             2778..4463
                     /locus_tag="OO332_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4501..6873
                     /locus_tag="OO332_003396"
     CDS             4501..6873
                     /locus_tag="OO332_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5943752.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6930..9713
                     /locus_tag="OO332_003397"
     CDS             6930..9713
                     /locus_tag="OO332_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5943753.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10075..>10466
                     /locus_tag="OO332_003398"
     CDS             10075..>10466
                     /locus_tag="OO332_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5943754.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEAC"
BASE COUNT         3084 a         2315 c         2266 g         2801 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact
     3661 ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc
     3721 gccaggcatt tatgctagta tcgaacaatt taagctggtt tatttataaa tatgacgaac
     3781 ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc actgaatagc
     3841 gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctaat gcgagtattc
     3901 gtacgcctga taataagatc atattagaga acttgaactt tcatgtttcg ccaggcaaat
     3961 ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa acattatccc
     4021 actgctggcc gtgatttaaa ggtgatattt cttctcctgc tgacagttgg tatgtgtcac
     4081 aaacaccgtt aatcaaaagc ggcttactga aagagattat ttgtaaagca cttcccctgc
     4141 ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtctgggg aaattggctg
     4201 cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa aaacaacgta
     4261 tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac gaaactacct
     4321 ctcatcttga ggaacaagag gctatacgct tactgcgttt agtgcgtgaa aaactaccca
     4381 caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggct gatgatattt
     4441 gtgacattag cacggtttta taacaacaat aaaagccctg ctgtacggag atataaataa
     4501 atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc
     4561 gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc tcctgtccct
     4621 gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt accgaccggg
     4681 aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa
     4741 agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca cggtgcgtcg
     4801 ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct gaacccagcg
     4861 aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg gtattatctt
     4921 gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt
     4981 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
     5041 gtgaaattgg gttatcgcac tacgcgtttg gactggttaa catcgcatat cgatgagaat
     5101 aacaagagcg catttaatca aggttcttca ggaagtacct atttctctcc agattttaaa
     5161 aagaactttt ataccttgtc gtttaatcag gagctggctg ataactttgg cgttaccgcc
     5221 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
     5281 gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
     5341 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
     5401 tataatacca gcaccttccc gcaatctgat cgcgaaatgg gtaataaatc ctatggtctg
     5461 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
     5521 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
     5581 tatggtgata ttacagggcg ttgtacccgt ggcggattag gacacatttc ccaggctgta
     5641 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
     5701 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
     5761 cagtctgaat cctatgtgat taatgctgca ggaaagaaaa ctaaccatac catttaccat
     5821 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga tcacattagc
     5881 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
     5941 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     6001 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     6061 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
     6121 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
     6181 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     6241 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6301 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6361 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6421 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6481 tacgatagcg ttccagtggc agattttaat accccattaa agatctcctt aaacatggat
     6541 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6601 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6661 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6721 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     6781 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     6841 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     6901 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     6961 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     7021 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     7081 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     7141 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     7201 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     7261 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     7321 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     7381 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     7441 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     7501 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     7561 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     7621 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     7681 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     7741 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     7801 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     7861 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     7921 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     7981 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     8041 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     8101 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     8161 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     8221 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     8281 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     8341 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     8401 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     8461 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     8521 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     8581 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     8641 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     8701 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     8761 accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg attaacgaca
     8821 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     8881 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     8941 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     9001 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     9061 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     9121 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     9181 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     9241 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     9301 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     9361 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     9421 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     9481 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     9541 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     9601 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     9661 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     9721 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     9781 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     9841 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgcca
     9901 ccacccaaaa attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     9961 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
    10021 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
    10081 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
    10141 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
    10201 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact
    10261 ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc cattaacaaa
    10321 aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaac
    10381 atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac
    10441 accattggtt cttccgaggc ctgtat
//
LOCUS       DAJTRQ010000129        10423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000129 DAJTRQ010000000
VERSION     DAJTRQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO332_003399"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO332_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5943755.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO332_003400"
     CDS             complement(1151..1237)
                     /locus_tag="OO332_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5943756.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO332_003401"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO332_003401"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5943757.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO332_003402"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO332_003402"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5943758.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO332_003403"
     CDS             3586..3630
                     /locus_tag="OO332_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943759.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO332_003404"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO332_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5943760.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OO332_003405"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OO332_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5943761.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OO332_003406"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OO332_003406"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5943762.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OO332_003407"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OO332_003407"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5943763.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OO332_003408"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OO332_003408"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5943764.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OO332_003409"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OO332_003409"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5943765.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..>10423)
                     /locus_tag="OO332_003410"
     CDS             complement(10298..>10423)
                     /locus_tag="OO332_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943766.1"
                     /translation="VILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV"
BASE COUNT         2726 a         2740 c         2547 g         2410 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccatc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aagcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaatgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 acccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccatttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattatt ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca ttaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat tac
//
LOCUS       DAJTRQ010000130        10349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000130 DAJTRQ010000000
VERSION     DAJTRQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            9..461
                     /locus_tag="OO332_003411"
     CDS             9..461
                     /locus_tag="OO332_003411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5943767.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DEFRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="OO332_003412"
                     /pseudo
     CDS             707..1751
                     /locus_tag="OO332_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1813..2427
                     /locus_tag="OO332_003413"
                     /pseudo
     CDS             1813..2427
                     /locus_tag="OO332_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="OO332_003414"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="OO332_003414"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5943768.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="OO332_003415"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="OO332_003415"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5943769.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="OO332_003416"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="OO332_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5943770.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /gene="crl"
                     /locus_tag="OO332_003417"
     CDS             6055..6456
                     /gene="crl"
                     /locus_tag="OO332_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5943771.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="OO332_003418"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="OO332_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5943772.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="OO332_003419"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="OO332_003419"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5943773.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="OO332_003420"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="OO332_003420"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5943774.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         2520 a         2548 c         2703 g         2578 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatttc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggttcat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcg
//
LOCUS       DAJTRQ010000131        10303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000131 DAJTRQ010000000
VERSION     DAJTRQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..179)
                     /locus_tag="OO332_003421"
     CDS             complement(<1..179)
                     /locus_tag="OO332_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5943775.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSG"
     gene            complement(317..1963)
                     /gene="groL"
                     /locus_tag="OO332_003422"
     CDS             complement(317..1963)
                     /gene="groL"
                     /locus_tag="OO332_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5943776.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2007..2300)
                     /locus_tag="OO332_003423"
     CDS             complement(2007..2300)
                     /locus_tag="OO332_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5943777.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2576..3832
                     /gene="yjeH"
                     /locus_tag="OO332_003424"
     CDS             2576..3832
                     /gene="yjeH"
                     /locus_tag="OO332_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5943778.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(3848..4324)
                     /gene="fxsA"
                     /locus_tag="OO332_003425"
     CDS             complement(3848..4324)
                     /gene="fxsA"
                     /locus_tag="OO332_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5943779.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            4661..6097
                     /gene="aspA"
                     /locus_tag="OO332_003426"
     CDS             4661..6097
                     /gene="aspA"
                     /locus_tag="OO332_003426"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5943780.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6215..7516
                     /gene="dcuA"
                     /locus_tag="OO332_003427"
     CDS             6215..7516
                     /gene="dcuA"
                     /locus_tag="OO332_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5943781.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7632..7970
                     /gene="cutA"
                     /locus_tag="OO332_003428"
     CDS             7632..7970
                     /gene="cutA"
                     /locus_tag="OO332_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5943782.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            7946..9643
                     /gene="dsbD"
                     /locus_tag="OO332_003429"
     CDS             7946..9643
                     /gene="dsbD"
                     /locus_tag="OO332_003429"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5943783.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9680..10255
                     /locus_tag="OO332_003430"
     CDS             9680..10255
                     /locus_tag="OO332_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5943784.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2402 a         2693 c         2570 g         2638 t
ORIGIN      
        1 tgcccggaaa gccagttgct ttgcttacct gtcgcagtac caatcagctg gcactctgcg
       61 cctggctgct cgtccacaat gcgtacactc tgaccggcag cactcaattc gttgctggag
      121 ctacaacccg ccatcagtag cgcggcaccg acaatccctg ctaagtattt tacgtgcatg
      181 ttattcccca taatcaatga gctggacgcc actcgcgtcg tccgtgtctg aatcttatac
      241 tagaaagatg accaaaagaa aaacccccag acatttctgc ccgggggttt gtttatttct
      301 gcgaagtgca gggcaattac atcatgccgc ccatgccacc catgccgccc ataccgccag
      361 cagcgcctaa gtcagctgca tcgtttttcg gcaggtcggt aaccatgcat tcggtggtga
      421 tcatcaggcc agccacagaa gctgcgtact gcagagcaga acgggttact ttggttgggt
      481 ccaggatacc catgtcgatc atgttgccgt attcttcggt tgctgcgttg taaccgtagt
      541 tgccgtcgcc gcctttaacg gtgttagcaa caacagacgg ttcttcgccg cagttcagga
      601 cgatctgacg cagcggagct tccattgcac gcagtgcaac tttgataccc acgttctggt
      661 cttcgttctg accacgcagg tcagccagtt tagacgctac gcggatcagc gcaacaccac
      721 caccagcaac cacgccttct tctaccgcag cacgggtcgc gtgcagggca tcttcaacgc
      781 gtgctttttt ctctttcatt tcaacttcgg tagcagcacc tactttgata actgcaacgc
      841 cgcctgccag tttcgctacg cgctcctgca gtttttcacg gtcgtagtca gaagttgctt
      901 cttcaatctg ctgacggatc tgagcaacac ggccctggat tgcagcttct tcacccacgc
      961 catcgatgat agtggtggtg tctttgttga tcacaacacg tttagcctga cccaggtctt
     1021 ccagggttgc tttttccagc tccataccga tctcttcaga gattacggta ccgccagtca
     1081 gggttgcgat atcctgcagc atagctttac gacgatcgcc gaagcccgga gctttaaccg
     1141 cagcaacttt cacgatgcca cgcatggtgt taacaaccag agttgctagc gcttcgcctt
     1201 caacatcttc agcgatgatc agcagcggtt tgcctgcttt ggcaacagct tccagaaccg
     1261 gcagcatttc gcggatgttg gagattttct tgtcagccag caggatgaac gggctttcca
     1321 gttctactgc gccagtttcc ggcttgttga tgaagtaagg agacaggtag ccacggtcga
     1381 actgcatacc ttcaaccacg tccagttcgt cctgcagacc ggtaccgtct tcaacggtga
     1441 taacgccttc tttaccgact ttgtccatcg cttcagcgat cagtttacct acggtttcgt
     1501 cggagttagc ggagatggta ccaacctgag caatcgcttt agaatcagag cacggtacgg
     1561 acagcgcttt cagttcttca actgcagcgg taaccgcttt gtcgatacca cgtttcaggt
     1621 ccatcgggtt catgcccgca gcaacagctt tcagaccttc agtgatgata gcctgagcca
     1681 gtacggttgc agtggtggta ccgtcgcctg cagcgtcgtt cgctttggag gcaacttctt
     1741 tcaccatctg cgcacccata ttttcgaact tgtcttccag ttcgatttca cgagcaacgg
     1801 aaacaccatc tttggtgatg gtcggtgcac cgaaagattt atccagaact acgttacggc
     1861 cttttggacc gagggtaact ttcactgcat ctgccagtac gtttacgccg cgcagcattt
     1921 tcacacgagc gtcgttaccg aattttacgt ctttagctgc cattatcttt attccttaaa
     1981 ttcgtatgtt cagtgtcgtg cgcggattac gcttcaacaa ttgccagaat gtcgctttcg
     2041 gacatgatca acacttcttc attgtcgatc ttctcggatt tcacaccgta gccatcgttg
     2101 aaaataacga tgtcgccaac tttcacgtcc agcggcttca cttcgccatt ttcaaggata
     2161 cggccattgc cgacagccag cacttcgccg cgggtggatt tagccgctgc agagccggtc
     2221 agaacgatgc cgccagcaga tttagtttca acttctttac gcttgacgat cacgcgatca
     2281 tgcaatggac gaatattcat tgataactct cctttgagaa agtccgtatc tgttatgggt
     2341 gacgccggag cttacgtggt ttcccggctg gtgaccagag aaatggggat gaggcttcgc
     2401 cccttcaagg gggaaaaaga aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg
     2461 attcacctca catttcatcg caatttcttc atcgccgtca taagcgaatc tgattgtgct
     2521 accatcgaaa atctacgcag ttgcccaaaa tttgggcgca atcggatatc aacccatgag
     2581 tggactcaaa caagaactgg ggctggccca gggcattggc ctgctatcga cgtcattatt
     2641 aggcactggc gtgtttgccg ttcctgcgtt agctgcactg gtagcaggca ataacagcct
     2701 gtgggcgtgg cccgttttga ttatcttagt gttcccgatt gcgattgtgt ttgcgattct
     2761 gggtcgccac tatcccagcg caggcggcgt cgcgcacttc gtcggtatgg cgtttggttc
     2821 gcggcttgag cgagtcaccg gctggctgtt tttatcggtc attcccgtgg gtttgcctgc
     2881 cgcactacaa attgccgccg gattcggcca ggcaatgttt ggctggcata gctggcaact
     2941 gttgttggca gaactcggta cgctggcgtt ggtgtggtat atcggtactc tcggtgccag
     3001 ttccagtgct aatctacaaa ccgttattgc cggacttatc gtcgcactga ttgtcgctat
     3061 ctggtgggcg ggcgatatca aacctgcgag tatccccttc cccgcgccag gaaatatcga
     3121 acttaccggg ttatttgctg cgttatcagt gatgttctgg tgttttgtcg gtctggaagc
     3181 atttgcccat cttgcctcgg aatttaaaaa tccagagcgt gattttcctc gtgctttgat
     3241 gattggtctg ctgctggcag gattagtcta ctggggctgt acggtagtcg tcttacactt
     3301 cgacgcctat ggtgaacaaa tggcggcggc agcatcgctt ccaaaaattg tagtgcagtt
     3361 gttcggtgta ggagcgttat ggattgcctg cgtaattggc tatctggcct gctttgccag
     3421 tcttaacatt tatatacaga gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc
     3481 tgaccactac ctggcacgcc tctcttctcg ccatatcccg aataatgccc tcaatgcggt
     3541 gcttggctgc tgcgtggtga gcacgttggt aattcatgcc ttagagatca atctggaggc
     3601 tcttattatt tatgccaatg gcatctttat tatgatttat ctgttatgca tgctggcagg
     3661 ctgtaaatta ttgcaaggtc gttatcgact actggcggtg gttggcgggc tgttatgcgt
     3721 tctgttactg gcaatggtcg gctggaaaag tctctatgcg ctgatcatgc tggtggggtt
     3781 atggctgttt ctgccaaaac gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt
     3841 ttcgacatta atcctggcga tcgtctttat gatcaaggcg gtcgcggtca tcatcctttc
     3901 gctgatactc accatcaaaa gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg
     3961 gcatgcgaga aaagcgcaaa tgtggcatca acttcactgt cagatgcttt tgcaccggcg
     4021 gcaataaaag cagaagaccg aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac
     4081 cggcaataat cagcgaaaca cttttaatca tctccgccgc agggttttca cccgccgcca
     4141 ttttttgctg catcagcacg aaattcttaa agccctggtt acgtaccagt gacataccga
     4201 taactgacgt gaatataacc agcacgaggg tcagcaatac ccccaataca tgggcaacct
     4261 gaataaagat tgaaatctca atatagacat aaaggaaaat ggcaataaaa ggtaaccagc
     4321 gcaaaggttt ctcctgtaat agcagccggt taaacccggc tacctgaatg ggttgcgaat
     4381 cgcgttcagc ttatattgtg gtcattagca aaatttcaag atgtttgcgc aactattttt
     4441 ggtagtaatc ccaaagcggt gatctatttc acaaattaat aattaagggg taaaaaccga
     4501 cacttaaagt gatccagatt acggtagaaa tcctcaagca gcatatgatc tcgggtattc
     4561 ggtcgatgca ggggataatc gtcggtcgaa aaacattcga aaccacatat attctgtgtg
     4621 tttaaagcaa atcattggca gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat
     4681 cgaagaagat ctgttgggta ccagggaagt tccagctgat gcctactatg gtgttcacac
     4741 tctgagagcg attgaaaact tctatatcag caacaacaaa atcagtgata ttcctgaatt
     4801 tgttcgcggt atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac
     4861 cattcctaaa agtgtagcga atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg
     4921 aaaatgcatg gatcagttcc cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa
     4981 catgaacacc aacgaagtgc tggccaatat cggtctggaa ctgatgggtc accagaaagg
     5041 tgaatatcag tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca ctaacgacgc
     5101 ctacccgacc ggtttccgta tcgcagttta ctcttccctg attaagctgg tagatgcgat
     5161 taaccaactg cgtgaaggct ttgaacgtaa ggctgtcgaa ttccaggaca tcctgaaaat
     5221 gggtcgtacc cagctgcagg acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt
     5281 cagcatcctg ctgaaagaag aagtgaaaaa catccaacgt accgctgaac tgctgctgga
     5341 agttaacctt ggcgcaacag caatcggtac tggtctgaac acgccgaaag agtactctcc
     5401 gctggcagtg aaaaaactgg ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct
     5461 gatcgaagcg acctctgatt gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct
     5521 ggctgtgaag atgtccaaaa tctgtaacga cctgcgcttg ctctcttctg gtccacgtgc
     5581 cggcctgaac gagatcaacc tgccggaact gcaggcgggc tcttccatca tgccagctaa
     5641 agtaaacccg gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga
     5701 caccactgtt accatggcag cagaagcagg tcagctgcag ttgaacgtta tggaaccggt
     5761 cattggccag gccatgttcg aatccgttca cattctgacc aacgcttgct acaacctgct
     5821 ggaaaaatgc attaacggca tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa
     5881 ctctatcggt atcgttactt acctgaaccc gttcatcggt caccacaacg gtgacatcgt
     5941 gggtaaaatc tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct
     6001 gttgactgaa gcggaacttg acgatatttt ctccgtacag aatctgatgc acccggctta
     6061 caaagcaaaa cgctatactg atgaaagcga acagtaatcg tacagggtag tacaaataaa
     6121 aaaggcacgt cagatgacgt gccttttttc ttgtgagcag taacttaaaa ataacaatct
     6181 aatatcaact tgttaaaaaa caaggaaggc taatatgcta gttgtagaac tcatcatagt
     6241 tttgctggcg attttcttgg gcgccagatt ggggggaata ggtattggtt ttgcaggcgg
     6301 attgggggtg ctggttcttg ccgctattgg cgttaaaccc ggtaacatcc cgttcgatgt
     6361 catttccatt atcatggcgg ttatcgccgc tatttctgcc atgcaggttg ctggcggtct
     6421 ggactatctg gttcatcaga cagaaaagct gctgcgccgt aacccgaaat acatcacgat
     6481 cctcgcaccg atcgtgacct atttcctgac tatctttgct ggtactggca acatctctct
     6541 ggcgacactg ccagttatcg ctgaagttgc gaaggaacaa ggcgttaaac cttgccgtcc
     6601 gctctctact gcagtggtat ccgcgcagat tgcgatcacc gcatcgccaa tctcagcggc
     6661 agtggtttac atgtcttccg tgatggaagg tcatggcatc agctacctcc atctgctctc
     6721 cgtggtcatc ccgtccaccc tgctggcggt tctggtgatg tccttcctgg tcactatgct
     6781 gttcaactcc aaactctctg acgatccgat ttatcgcaag cgtctggaag agggcttggt
     6841 tgaactgcgc ggtgaaaagc agattgaaat caaatccggt gcaaaaacgt ccgtctggct
     6901 gttcctgctg ggcgtagttg gcgtggttat ctatgcaatc atcaacagcc caagcatggg
     6961 tctggttgaa aaaccgctga tgaacaccac caacgcaatc ctgatcatca tgctcagcgt
     7021 tgcaactctg accaccgtta tctgtaaagt cgataccgac aacatcctca actccagcac
     7081 cttcaaagca ggtatgagcg cctgtatttg tatcctgggt gttgcgtggc tgggcgatac
     7141 tttcgtttcc aacaacatcg actggatcaa agataccgct ggtgaagtga ttcagggtca
     7201 tccgtggctg ctggccgtca tcttcttctt tgcttctgct ctgctgtact ctcaggctgc
     7261 aaccgcaaaa gcactgatgc cgatggctct ggcactgaac gtttcaccgc tgaccgctgt
     7321 tgcttctttc gctgcggtgt ctggtctgtt cattctgccg acctacccga cgctggttgc
     7381 tgcggtacag atggatgaca cgggtactac ccgtatcggt aaattcgtct tcaaccatcc
     7441 gttcttcatc ccgggtactc tgggtgttgc cctggccgtt tgcttcggct tcgtgctggg
     7501 tagcttcatg ctgtaatgat caatcgcggg gcgttcacgc cccgctttct ttcccgccga
     7561 ctaacatcct tcccccgtcc gttgtatagt gacctctctc ttgcggttcc atctgttctt
     7621 gcgaggtgtt tatgcttgat gaaaaaagtt cgaataccgc gtctgtcgtg gtgctatgta
     7681 ccgcaccgga tgaagcgaca gcccaggatt tagccgccaa agtgctggcg gaaaaactgg
     7741 cggcctgcgc gaccttgatc cccggcgcta cctctctcta ttactgggaa ggtaagctgg
     7801 agcaagaata cgaagtgcag atgattttaa aaactaccgt atctcaccag caggcactgc
     7861 tggaatgcct gaagtctcat catccatatc aaaccccgga acttctggtt ttacctgtta
     7921 cacacggaga cacagattac ctctcatggc tcaacgcatc tttacgctga ttctgctact
     7981 ttgcagcact tccgtttttg ccggattatt cgacgcgccg ggacgttcac aatttgtccc
     8041 cgcggatcaa gcctttgctt ttgattttca gcaaaaccaa catgacctta atctgacctg
     8101 gcagatcaaa gacggttact acctctaccg taaacagatc cgcattacgc cggaacacgc
     8161 gaaaattgcc gacgtgcagc tgccgcaagg cgtctggcat gaagatgagt tttacggcaa
     8221 aagcgagatt taccgcgatc ggctgacgct tcccgtaacc atcaaccagg cgagtgcggg
     8281 agcgacgtta actgtcacct accagggctg tgctgatgcc ggtttctgtt atccgccaga
     8341 aaccaaaacc gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac agcctgtgtc
     8401 tgtttcgcag caagagcagc acaccgcgca attgcccttt tccgcgctct gggcgttgtt
     8461 gatcggtatt ggtatcgcct ttacgccatg cgtgctgcca atgtacccac tgatttctgg
     8521 catcgtgctg ggtggtaaac agcggctctc cactgccaga gcattgttgc tgacctttat
     8581 ttatgtgcag gggatggcac tgacttacac ggcgctgggt ctggtagttg ccgccgcagg
     8641 gttacagttc caggcggcgc tacagcaccc atacgtgctc attggcctcg ccatcgtctt
     8701 taccttgctg gcgatgtcaa tgtttggctt gtttactcta caactcccct cttcgctgca
     8761 aacacgtctc acgttgatga gcaatcgcca acagggcggc tcacctggcg gtgtgtttgt
     8821 tatgggggcg attgccggac tgatctgttc accatgcacc accgcaccgc ttagcgcgat
     8881 tctgctgtat atcgcccaaa gcgggaacat gtggctgggc ggcggcacgc tttatctcta
     8941 tgcgttgggc atgggcctgc cgctgatgct aattaccgtc tttggtaacc gcttgctgcc
     9001 gaaaagcggc ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact
     9061 gccggtcttc ctgctggagc gagtgattgg tgatgtatgg ggattacgct tgtggtcggc
     9121 gctgggtgtc gcattctttg gctgggcctt tatcaccagc ctacaggcta aacgcggctg
     9181 gatgcgcgtg gtgcaaataa tcctgctggc agcggcattg gttagcgtgc gcccacttca
     9241 ggattgggca tttggtgcga cgcataccgc gcaaactcag acgcatctca actttacaca
     9301 aatcaaaacg atagatgagt taaatcaggc gctcgttgaa gccaaaggca aaccggtgat
     9361 gttagatctt tatgccgact ggtgcgtcgc ctgtaaagag tttgagaaat acaccttcag
     9421 cgacccgcag gtgcaaaaag cgttagcaga cacggtctta cttcaggcca acgtcacggc
     9481 caacgacgca caagatgtgg cgctgttaaa gcatcttaat gtccttggcc taccgacaat
     9541 tctctttttt gacggacaag gccaggagca tccacaagca cgcgtcacgg gctttatgga
     9601 tgctgaaacc ttcagcgcac atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg
     9661 aaagacggag gaaaatagcg tgcaacgtga agatgtactg ggagaagccc tgaaattatt
     9721 agaattacaa gggattgcca acaccacgct ggagatggtt gctgaacgtg tggattatcc
     9781 actggacgag ctacgccgct tctggccaga caaagaggca atcctctacg atgcgctgcg
     9841 ttatcttagc caacagatag atgtctggcg tcgtcagctg atgctggacg aaacgcaaac
     9901 cgccgaacaa aagctgctgg cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg
     9961 ctatccgggc tgtctattta tcgctgcctg tacgttttat cccgatcctg gccaccctat
    10021 tcatcaactg gccgatcagc aaaaaagcgc ggcctacgat ttcacccacg aactgttaac
    10081 cacgctggaa gttgacgatc cggcgatggt agcaaagcag atggaactgg tgctggaagg
    10141 ctgtttaagc cgaatgctgg tgaatcgtag ccaggcggat gtcgacactg cacatcggct
    10201 ggcggaagat atcctgcgct tcgcccgctg ccgccagggt ggtgcattga cctgacagaa
    10261 acacagaaaa gaagcgattt gccgcaatct taagcagttg aat
//
LOCUS       DAJTRQ010000132        10257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000132 DAJTRQ010000000
VERSION     DAJTRQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="OO332_003431"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="OO332_003431"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5943785.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="OO332_003432"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="OO332_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5943786.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="OO332_003433"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="OO332_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5943787.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3187..4124
                     /locus_tag="OO332_003434"
                     /pseudo
     CDS             3187..4124
                     /locus_tag="OO332_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="OO332_003435"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="OO332_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="OO332_003436"
     CDS             5927..6439
                     /locus_tag="OO332_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5943788.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="OO332_003437"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="OO332_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5943789.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="OO332_003438"
     CDS             9032..9235
                     /locus_tag="OO332_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5943790.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="OO332_003439"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="OO332_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5943791.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2446 a         2670 c         2650 g         2491 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga acacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga tcccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcagtc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagatccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tattcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtggaa
//
LOCUS       DAJTRQ010000133        10181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000133 DAJTRQ010000000
VERSION     DAJTRQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            291..1007
                     /locus_tag="OO332_003440"
     CDS             291..1007
                     /locus_tag="OO332_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5943792.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1463..1538)
                     /locus_tag="OO332_003441"
     tRNA            complement(1463..1538)
                     /locus_tag="OO332_003441"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1503..1505),aa:Asn,seq:gtt)
     gene            complement(1639..3266)
                     /gene="yeeO"
                     /locus_tag="OO332_003442"
                     /pseudo
     CDS             complement(1639..3266)
                     /gene="yeeO"
                     /locus_tag="OO332_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3271..3346
                     /locus_tag="OO332_003443"
     tRNA            3271..3346
                     /locus_tag="OO332_003443"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3304..3306,aa:Asn,seq:gtt)
     gene            complement(3384..4334)
                     /gene="cbl"
                     /locus_tag="OO332_003444"
     CDS             complement(3384..4334)
                     /gene="cbl"
                     /locus_tag="OO332_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5943793.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4436..5353)
                     /gene="nac"
                     /locus_tag="OO332_003445"
     CDS             complement(4436..5353)
                     /gene="nac"
                     /locus_tag="OO332_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5943794.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5680..5755
                     /locus_tag="OO332_003446"
     tRNA            5680..5755
                     /locus_tag="OO332_003446"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5713..5715,aa:Asn,seq:gtt)
     gene            complement(5811..6746)
                     /gene="ldtA"
                     /locus_tag="OO332_003447"
     CDS             complement(5811..6746)
                     /gene="ldtA"
                     /locus_tag="OO332_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5943795.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(6808..7887)
                     /gene="cobT"
                     /locus_tag="OO332_003448"
     CDS             complement(6808..7887)
                     /gene="cobT"
                     /locus_tag="OO332_003448"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5943796.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7899..8631)
                     /gene="cobS"
                     /locus_tag="OO332_003449"
                     /pseudo
     CDS             complement(7899..8631)
                     /gene="cobS"
                     /locus_tag="OO332_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8628..9173)
                     /gene="cobU"
                     /locus_tag="OO332_003450"
     CDS             complement(8628..9173)
                     /gene="cobU"
                     /locus_tag="OO332_003450"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5943797.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9800..10063)
                     /locus_tag="OO332_003451"
                     /pseudo
     CDS             complement(9800..10063)
                     /locus_tag="OO332_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2894 a         2582 c         2158 g         2547 t
ORIGIN      
        1 ctggcaaata gcctgcaata aacgcgggct gcttgcttcc ttgggcagat gcgagtttta
       61 ttatcgtctt aatgatttcc acatattaaa agcaagtatg ctttcaaaac acaattataa
      121 aaaatcccgc caacaacatt agtttttata aaattaaata taagattatg gctttagaat
      181 atttttattt ctaatagacg agatttttcc tgttatgata taatatgccg aattaacaca
      241 tgttaacgat ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta
      301 aatgggccaa tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg
      361 caaaattcgg tgtagaaatc tatgctgctg ccaaacaagg tgaacccgat ccagaattaa
      421 acacatcttt aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta
      481 ttgataaagc aattgataaa gccaaaggcg gcggagatga aacgttcgta cagggacgtt
      541 atgaaggctt tggtcctaat ggctcaatga ttattgctga gacattgact tcaaatgtta
      601 accgtacgat tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg
      661 caggttctgt cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggaacagacc
      721 ctgaccatat ttttgaaatt ttgcttgacg ctgaagttga tgttcgtgat gtaactgaag
      781 aagaaggtaa tattgttatt tatactgaac ctgctgacct tcataaagga atcgcggcgc
      841 taaaagcagc tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg
      901 aagttgagct ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag
      961 atgacgacga tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tattttaaag
     1021 aaatctgtct ttatggcaga tttctttaat ctcatataat tcttataaaa aatataatgt
     1081 ttaactcgtc atattgctta taaaaaatcc ccgttcccag agaaataata tttattaaaa
     1141 tggcaggtct tctttttctg attaccgaag acaaagtggc aattacgtat agtttcccga
     1201 taaagacgcg acagctacat cccgcataag gcatttttct ctttatcttt gtacggtact
     1261 tcatggaaca gaatttttga ccttgtgaat cgtgatgtct gttggggagg ggcaatttgc
     1321 tcactgaagc gtgagactcg attaagcgca cgaaacacag aaatcaaaaa acccggccac
     1381 ttttttacaa agtggccggg taaaaataat ttttattttt taactgtcct gagtctcata
     1441 gagatgtctc aaaactaaaa tttggctcct ctgactggac tcgaaccagt gacatacgga
     1501 ttaacagtcc gccgttctac cgactgaact acagaggaat cgtgagaacg aggcgaatat
     1561 tagcgatgcc cacccacaat gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa
     1621 taatctgcat tttgtcgttt attccgacac aactggcttt ttttcacact tttgcggctc
     1681 ggatcgaggg tatttccaca gccaacgtcc agtaaccatt cgccagtaaa acagcacggc
     1741 ccgcacagcc cagtaggcaa acattcccat ccagacacca accacacccc agccaagcat
     1801 gattccctgc acataaccga ctacaacccg gcaaccccac atgctcaaca tagaaaccca
     1861 catggcgtaa cgggcatcac gagcaccctt aaatccagcg ggtagcaccc atgaggcgga
     1921 ccaaataggc ataaataaag catttagcta aatcagaatc acaacgacgt gtttaacctg
     1981 tggatcctgg gtgtaaaacg atgccataac cccggcaaag ggagccgtta gccaggcgat
     2041 ggccgttaat ccaagagtgg acagccagaa cacatgccgc aactgaatct ctgcttgcgc
     2101 tatctgccct acccccaacc ttcggcctgt aatgatcgta gaagcagaac cgagcgcact
     2161 tccgggtaag ttgataagag ccgcaattga gaacgcgata aaatttccgg caataacgct
     2221 ggtccccatc ccggcaacga acatttgggt taataaccga ccactggtaa ataacactga
     2281 ttcgacactc gctggaatac caatccccat gacttcccag ataatgctaa aattcagcgg
     2341 tttaaaatag ctctttaacg aaatccttag cgcaggatta aaaccaatcg ccagcaccca
     2401 caaaattgca actgcgccaa tataacgaga aatggttaaa cccaacccag ccccgaccag
     2461 gagaaaaggc cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg
     2521 ttaatcagta gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaataaga
     2581 gtgatggcag cggctggata actgagtacg gtcagctcca gataagtcaa cgccagtgct
     2641 ttaacttctg tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc aaaatgatga
     2701 attagcgttg ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc
     2761 accctcgctc gtcgtcgatc ccgcttaccg agactaaatg ctacaacgac agtagtacca
     2821 agatcgatag ctgcaaaaaa agccataatg accatattga agctgtccgc caaccccacg
     2881 ccggccatcg catcttttcc cagccagctg accagaaaag tgctcaggcc ccccatcaac
     2941 aggacacaga cgttctccat gaagatagga acagcaagcg gggttatctc gcgccagaac
     3001 aacactttgt agctcttgcg tttagagtgc cagcgagtgc cgtgaacaac ctggcgtaaa
     3061 gcagaggaga tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaatgat
     3121 gagggagaat cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa
     3181 actgttgata gaaacggcaa acagttggga aatttaaaaa tcgggtttga caaaatattt
     3241 ttcgccgtta agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg
     3301 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt tctgaaaagc
     3361 ccgcttttat agcgggcttt ttgctatatc tgataatcaa tttcctcttc actgttttcc
     3421 atcacctggc gcttgatatc ctcaactgac agtcctgcat tacaaagttc cagaaagcgc
     3481 caaacatagt tacgctgaag ttgtcctcgc ttcagtccca accagacagt attagcatcg
     3541 aaaagatgcc gcgtatccag gcggattaaa ttctcttcct cttgttcgcc gctggattgt
     3601 tcggcaacta atccgatccc aagcccaaga gcaacatagg ttttaatgac atcagaatcc
     3661 tgtgcactta atacaatatc tgccggcaaa cctttgcggg taaatgcgtc atcagtgcgt
     3721 gagcgccccg taattccctg tcggtaagta attaacggcc acttcgctat tgattccagc
     3781 gtcaatggtg taatttgcgt taagggatga tcgagtggaa caagcaaact atggtgccaa
     3841 caaaaccacg ggaaggcgac gagctgcggg tcattactca aacgctcgct ggcgatacca
     3901 atatcagctt cgccattttg caacaacgtc gcaatttcct gtggcgaacc ctggattagc
     3961 tcgagccgaa cctccgggaa aagttcgcga aaagctttaa tgacctctgg caagctataa
     4021 cgtgcctgag tatgcgtcgt tgcaatagtg agaacaccag acgtatcgtt ggtaaacagg
     4081 tctgcaagcc gacgaacatt actggcttca ttcagaatac gttctgcaat gaccagtaat
     4141 gctttgcccg gttcagtcat ccccagcagt cgcttacctc gtcgaacaaa tatttcgatg
     4201 ccaagttcat cctccagttc ccgaatatga cggctaacgc ctgactgtga ggtaaaaagc
     4261 atattcgcaa cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat tatctttagt
     4321 tgttggaaat tcacggtaaa ctccgggcag ttcagatatc ccgttattgt taaagtctaa
     4381 tgcccggcat aacaaataat aaaaacccgc atcttattcc atcccgatat aacgcttagc
     4441 tcaccaattg ccactgcctt ttttccatca ctggagaact aatcactgac attaacaact
     4501 ctttcactgc ctgtgcctgt ggtgacaagt ttgctctggc gggtaaattt aaggacaaag
     4561 agagactcat tgaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg
     4621 aacgcgcagc cgattcaggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa
     4681 gcgtggcaat agactcaatt tcaccaatga cttttgccgt gagccgccgt agggaaaaag
     4741 cctcatcaac acgaagtcta acagcgctgt aatcactggg gagaaaaagg ttcatttgcg
     4801 caatagcatt aacatcaacg ttttgccccg ggcaatcttg agttcctacc aggaaaagat
     4861 cttctttcag caaagcctga ctggataccc cagccacagg ggaatgctca taaatcaccg
     4921 ccatatcgag ttggtgattt atcagttttt cgttaagcac tgcgccacta ttttcatgaa
     4981 gatagataac gacctccggg aattcagcgc gaaccgcctg taataagggc atggtgatgg
     5041 atgacgcagc ggttcctggc gcaaagccaa tcgagacttg ccccgataat gactgaccaa
     5101 cgttatgcac cgccagttgg gcctgctcac actgacgtaa aatggcccgt gcatgggtat
     5161 agagaatttt tccggcgtct gttggcgtaa cgccccgttt tgtgcgaatc aaaagctgtt
     5221 gatttaactc accttccagt atggcaacct gctggctgag cgctggttgt gcgatatgca
     5281 atacttcagc agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc
     5341 gtctgaagtt catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac
     5401 aaccagtatg caagatgcct gccagttcta ttttggcgtt gatgcgcaag ctaaccaact
     5461 gtcaaataag agattatgat agattcgtca ttggcccttt ttatcagcag tcgcattcac
     5521 ctgccctata aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct
     5581 caccactttg ttgaaattgg cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc
     5641 accatgcact gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta
     5701 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc
     5761 aaaaaagcct gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc
     5821 tgtcttgaac cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt
     5881 gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc
     5941 aatggcactt ttcggtcaga ctcatattct gcacgattgc gtgacagcgg ctcatgaact
     6001 tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt
     6061 acacgtgtgc ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga
     6121 atacagccct gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca
     6181 tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca
     6241 aatgcgggca aactctcccc tcgtttcgca tattcgcgcc gggtgttcgg cgttggcgtc
     6301 caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc
     6361 ccagcctggc cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa
     6421 taaagacgca tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc
     6481 agttgctgcg gaatggtgag ttgtgagcca ggcttcggta aaaaaacatc agcgcccgga
     6541 ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc
     6601 tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct
     6661 ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag
     6721 caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa
     6781 gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg
     6841 tagaacaata ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc
     6901 gatgatggac atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag
     6961 ataaggctcc agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg
     7021 agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc
     7081 ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac
     7141 tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg
     7201 aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt
     7261 atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt
     7321 gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag
     7381 cgtgacaccg tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc
     7441 agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg
     7501 catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac
     7561 gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc
     7621 agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg
     7681 atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg
     7741 cattcctgcc agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgag
     7801 taacccgtca acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt
     7861 attcagtaaa tcggcaatta tttgcatgtc atgtctcttt atagcagtgc cagcaggaag
     7921 accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta
     7981 cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca
     8041 ccatgcatac ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag
     8101 gtttgtcgcg ccaatgaaca cattgccaag cccttcttcc cgcgcataac gatgacgata
     8161 catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat
     8221 cggttcgcca cgcagagcca actcgcttaa caccagaatc tttgccagta ccacaaaaat
     8281 caatgccaga ccgccgtggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc
     8341 gcggctacgt gcagaaaata cgccatcaca ggtatcagcg aggccgtcca gatggaattc
     8401 cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggtg cgccacacca
     8461 tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc caatcaaagg
     8521 aaaagtaata ataccgcgag aataatgctc gaaatccagt ccctgggacc agcgacgcgg
     8581 tacaggcaga cgcgtaataa atgagagcat cgcccaaaat aatttgctca tttgattttt
     8641 actccaatac ccgaaaccac cagccatact tcatttgccg cagcggccaa ctgctgattt
     8701 acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt
     8761 cccacttcgt tagtcactaa tacaaccttt gcggggcaac gttggcaggc agcaatcaac
     8821 gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc atcagggtct
     8881 ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaac
     8941 acagcctcat tcgggttaat gtctgcatga attaattcat caagatgttg ccagcgctcc
     9001 actgtgcgcc agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc agccatctca
     9061 tcatcaagga tttgcgaggt agcgatatac agaacctgtg aagagtcccc aataagcacc
     9121 tctgcatggc gactcttccc gctccatgcg cctcccgtca ccaaaatcat cataccgact
     9181 cctgattcaa atgtatgatg gtatacattg aacagagcaa tggatccccc accgttgctg
     9241 accgcaaaac atttacgcga ttaatactgc gcgtaatata attaaaaatc cagcattctc
     9301 aatccattac gaaataagat ggcattacgg aatagccaga ctttctcgtg ttaacgtccg
     9361 acaaaatgcg tcattgacag cggtttctgt ggattgacac atttcattca gataatgaat
     9421 taatgctact gccggaccaa ccagcaaacg ggcattgtgt tctgaaatcc aggctattga
     9481 ttcaacgtca gcgacagatc tgcgctgtaa aagaacttgt aaaacaacgc aatagaatta
     9541 tccggaatgg tggcgactat gcactagaac accacaattt cgctctctcg gtaaggcact
     9601 gtcacagcat tcaacagaat gtgacttgcc agatttatta gcgccaccag atgtttaacc
     9661 gggtaaccac gacccagtcg gggatatatt ccatcttctc gctgacatca tcaagaataa
     9721 aaaggtttat cacactcaga gcaacgttct tcagcaggcg ggataattct tcaacattta
     9781 aaagatgtgt tattaaaggc atctcaaatt ggacagggct gccggttttc tgttgcaaca
     9841 ccgttgctct gcttgagaca gaactgccat atgcagcatc tgatactttg agtttgacca
     9901 aatcacagcc ccgtagctta ctgtccaaag ccatgttgaa cagagctaga tcgcgtgttt
     9961 taccttccaa ctcaagtcta atccggatcc cccagatatg agatatctga agtggccttt
    10021 tttgaccgat ggtacggtct ttattccacg gtgacgtttt catattcaaa tctcctgcaa
    10081 tgtgggagat ttgagtatgg ttgtctctca agagcgaaaa taagtcaact atcactagtt
    10141 gttgcgcatg tacgtattct gtcaattaat tattgacaat c
//
LOCUS       DAJTRQ010000134         9957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000134 DAJTRQ010000000
VERSION     DAJTRQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..272)
                     /locus_tag="OO332_003452"
     CDS             complement(<1..272)
                     /locus_tag="OO332_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5943798.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO332_003453"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO332_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO332_003454"
     CDS             complement(1132..1338)
                     /locus_tag="OO332_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5943799.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO332_003455"
     CDS             complement(1445..2008)
                     /locus_tag="OO332_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5943800.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO332_003456"
     CDS             complement(3383..5218)
                     /locus_tag="OO332_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943801.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO332_003457"
     CDS             5319..5606
                     /locus_tag="OO332_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5943802.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO332_003458"
     CDS             5578..7107
                     /locus_tag="OO332_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5943803.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO332_003459"
     CDS             complement(7277..8494)
                     /locus_tag="OO332_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5943804.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="OO332_003460"
     CDS             complement(8861..9847)
                     /locus_tag="OO332_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943805.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..>9957)
                     /locus_tag="OO332_003461"
     CDS             complement(9844..>9957)
                     /locus_tag="OO332_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5943806.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3138 a         2262 c         1846 g         2711 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttc
//
LOCUS       DAJTRQ010000135         9785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000135 DAJTRQ010000000
VERSION     DAJTRQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO332_003462"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO332_003462"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5943807.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO332_003463"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO332_003463"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5943808.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="OO332_003464"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="OO332_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="OO332_003465"
     CDS             3456..5216
                     /locus_tag="OO332_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5943809.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="OO332_003466"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="OO332_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5943810.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="OO332_003467"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="OO332_003467"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5943811.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="OO332_003468"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="OO332_003468"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5943812.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2507 a         2451 c         2348 g         2479 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgct
//
LOCUS       DAJTRQ010000136         9774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000136 DAJTRQ010000000
VERSION     DAJTRQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..579
                     /locus_tag="OO332_003469"
     CDS             <1..579
                     /locus_tag="OO332_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5943813.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO332_003470"
                     /pseudo
     CDS             597..1235
                     /locus_tag="OO332_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO332_003471"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO332_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5943814.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO332_003472"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO332_003472"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5943815.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO332_003473"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO332_003473"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5943816.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO332_003474"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO332_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5943817.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO332_003475"
     CDS             complement(5552..6613)
                     /locus_tag="OO332_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5943818.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO332_003476"
     CDS             complement(6610..8007)
                     /locus_tag="OO332_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5943819.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO332_003477"
     CDS             8163..9161
                     /locus_tag="OO332_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5943820.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..>9774)
                     /gene="ompG"
                     /locus_tag="OO332_003478"
     CDS             complement(9272..>9774)
                     /gene="ompG"
                     /locus_tag="OO332_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR5943821.1"
                     /translation="RPDWDVKLTDDLRFNGWLSMYKFANDLNTTGYADTRVETETGLQ
                     YTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDR
                     WSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGV
                     GVNYSF"
BASE COUNT         2474 a         2599 c         2345 g         2356 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagcgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcataggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agctccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtaacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggg cgcg
//
LOCUS       DAJTRQ010000137         9511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000137 DAJTRQ010000000
VERSION     DAJTRQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..276
                     /locus_tag="OO332_003479"
     CDS             <1..276
                     /locus_tag="OO332_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5943822.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            432..1967
                     /gene="gadC"
                     /locus_tag="OO332_003480"
     CDS             432..1967
                     /gene="gadC"
                     /locus_tag="OO332_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5943823.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2098..3417
                     /locus_tag="OO332_003481"
     CDS             2098..3417
                     /locus_tag="OO332_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5943824.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3703..3744
                     /locus_tag="OO332_003482"
     CDS             3703..3744
                     /locus_tag="OO332_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943825.1"
                     /translation="MVQLVDLARCVSF"
     gene            3782..4927
                     /gene="dosC"
                     /locus_tag="OO332_003483"
     CDS             3782..4927
                     /gene="dosC"
                     /locus_tag="OO332_003483"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5943826.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4852..5469
                     /locus_tag="OO332_003484"
                     /pseudo
     CDS             4852..5469
                     /locus_tag="OO332_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5727..6059
                     /gene="ddpX"
                     /locus_tag="OO332_003485"
                     /pseudo
     CDS             5727..6059
                     /gene="ddpX"
                     /locus_tag="OO332_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6073..6969
                     /locus_tag="OO332_003486"
     CDS             6073..6969
                     /locus_tag="OO332_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5943827.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6966..7952
                     /locus_tag="OO332_003487"
     CDS             6966..7952
                     /locus_tag="OO332_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5943828.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7945..8871
                     /locus_tag="OO332_003488"
                     /pseudo
     CDS             7945..8871
                     /locus_tag="OO332_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="OO332_003489"
     CDS             complement(8927..9358)
                     /gene="osmC"
                     /locus_tag="OO332_003489"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5943829.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2317 a         2330 c         2393 g         2471 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacat acctgataac gtttaacggt aacggtgtcc
      301 cgaaacgaac ccgtttcggg ataatttcca aagtctgttc actggcatta gcaacggaaa
      361 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
      421 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
      481 gattctttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
      541 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
      601 tgggactttg tgctgcggaa atggccaccg tcgacggctg ggaagaaggt ggtgtcttcg
      661 cctgggtatc aaatactctg ggtccgagat ggggatttgc agcgatctca tttggctatc
      721 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
      781 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
      841 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
      901 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
      961 tttacctgca ctccggtgcc cccgttgcta tcgaaatgga ttcgaagacc ttcttccctg
     1021 acttctctaa agtgggcaca ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     1081 tagaagcttc cgcaactcac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     1141 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     1201 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     1261 ttctgatgtc ccatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     1321 tgttgctggg tgttctggcg gaaatcgcct cctggattgt tggtccttct cgcgggatgt
     1381 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     1441 taccggtaac gctggtcatt tcgcagctgg tgattacttc tatcgcgttg atcatcctca
     1501 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     1561 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     1621 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcaa
     1681 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     1741 tccagggtga ttctaccgat atgtatgttg aattactggt tgttagtttc ctggtggtac
     1801 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aggcaaagcg aataccggcg
     1861 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     1921 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     1981 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgct gtcgtattga
     2041 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     2101 gatatctgct cccgaaacaa gaaattaacg attagaagac cagcgatact ggttgcattg
     2161 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagca
     2221 ggttcaaagc caccagccac gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     2281 gccacggttt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taatcccacc
     2341 agccgagccc gtgtacaaca acaggcgatg atcgacaaac tggatcatct ccaacgtctc
     2401 ggcataaaca cggtcttttt ccaggtcaag ccggacggta ccgccctgtg gccatcgaaa
     2461 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     2521 ctgcaattca tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     2581 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     2641 tctcaacaac cggcgagcgt ctatgtgcaa caccgtgact ggatcagaac gtctggcgat
     2701 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgct
     2761 gaagtggttt cccgctatcc ggtagatggc gtgcagtttg acgactattt ctatactgaa
     2821 tcaccgggtt cacggctaaa tgataacgaa acgtaccgta aatacggagg cgcatttgcg
     2881 tcaaaagcag actggcggcg caacaatact cagcagttaa ttgcaaaggt atcacacacc
     2941 attaaaagca ttaagccggg agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     3001 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     3061 gctgacaccc gtcgatgggt ggaacaagga ttgctggatt acattgctcc ccaaatttac
     3121 tggccgttct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     3181 gttaaaccga ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     3241 tcaaagatag agccagactg gatgattaac ggcggcgtac cggaactgaa aaagcagctc
     3301 gatcttaacg atgcggtacc agaaattagc ggcaccatct tgttccgtga ggactatctg
     3361 aataaaccgc agactcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     3421 accatgctta ctttgtaaca agccggtgct ttacccaccg gcttgttaca ccttgtgaaa
     3481 tatcccacta gtaatcatgc ttacataagt caaattaacc acacttagtt aaacagctat
     3541 aaatccctaa taaaaatgta tgaatatgaa aaaccatacg atattcgctg tcaccgaaaa
     3601 ataattacga tgggctatac tcttctgaag aacagtagcg tagattgaaa ctgtcttaag
     3661 tcgcttattt cttttcagta tatcttcata tttcaggaga atatggtgca gttagtcgat
     3721 ctcgcacgtt gcgtttcgtt ttgattccgt ctgatttccc agccttataa gggtgtgaat
     3781 tatggagatg tattttaaaa gaatgaaaga tgagtggacc ggacttgtcg aacaggcaga
     3841 tcctctcatt cgtgctaaag ccgcagaaat tgcccttgcg catgctcatt atctgagtat
     3901 cgagttttat cgaattgtcc gcatcgaccc gcatgccgaa gaattcttga gtaatgaaca
     3961 agttgagcgg cagttgaaaa gtgcgatgga acgctggatt attaacgtgc tttctgccca
     4021 ggttgacgat gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag tgcatgcccg
     4081 cataggaatt ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa aaatcctcta
     4141 tccggtaatc ttctcttcgg attattccgc cgcagaaaaa cttcaggtct accatttctc
     4201 gattaacagt attgatatcg cgatggaagt gatgacccgc gcgtttacct ttagtgacag
     4261 tagtgcctca aaggaagatg aaaactatcg tatcttctcg ttactggaaa acgccgaaga
     4321 agaaaaagaa cggcaaatag cctcaatact ttcatgggaa atagatatta tctataaagt
     4381 cctgctggat tctgatttag gcagcagttt gcctttaagc caggctgatt ttggcctgtg
     4441 gtttaaccat aaaggtcgac attattttag cggcattgcc gaagtgggcc atatctcccg
     4501 tctgattcag gatttcgacg gtattttcaa tcaaaccatg cgtaacacca ggattttgaa
     4561 taacagaagt ttgcgggtga aatttctatt acagataaga aataccgtat cgcagattat
     4621 taccttgctg cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta tggatgtact
     4681 gacgaaatta cttaaccgcc gtttcctgcc gactatcttc aaacgcgaaa ttgcccatgc
     4741 caaccggacc ggtacaccgc tgtcagtgct gattattgac gttgataaat tcaaagagat
     4801 caacgatacg tggggccata acactggcga tgaaattctg cgtaaagtct cattcctctc
     4861 gcagaagaga ttggtgaaat cgaaaatatt gggcgctggg tcatcgcgga agcttgccgt
     4921 cagttagcag aatggcgtag ccagaatatt catatcccgg cgttatccgt gaacttgtcg
     4981 gcgctgcact ttcgcagtaa tcagctgcct aatcaggtgt ctgatgcaat gcaagcctgg
     5041 ggtattgacg gccaccagct gacggtggaa atcacggaaa gcatgatgat ggaacacgat
     5101 accgaaatct ttaagcgcat tcagatcctg cgtgatatgg gcgtgggttt atcggtagat
     5161 gattttggca cgggcttttc cggattatcc cgcttagtca gtcttccggt aacggaaatc
     5221 aaaattgaca aaagctttgt cgatcgttgt ctgacggaaa aacgcatcct ggccttactt
     5281 gaagccatta ccagcattgg gcaaagcctc aatttaaccg tcgtggcgga aggggtcgaa
     5341 accaaagagc aatttgagat gctacgtaag atccactgtc gcgttattca gggatatttc
     5401 ttttcccgcc cccttcccgc cgaagaaatt ccaggctgga tgagcagcgt gttaccgctg
     5461 aaaatctgac aaattcctct cgcccgcact cgcgggtttt cctttaaggt gacactgtca
     5521 cttaaatact gtgatttcag ctacgattcc gggagattcc ttccttaact ttccctcttg
     5581 caccaaaact gcactacgct gcacaaatgt tgatcaaaag ttaaaaaata taactttaag
     5641 ccagcattct ggcgcttatc ccggcatggc atgagatctg cataagcgga aagcgcagca
     5701 atttttgtct tatacaggaa ccgtttatgt cggataccac cgaactggtt gatttagccg
     5761 tgatcttccc tgatctggag atcgaattga aatatgcctg cgttgataac atcacaggta
     5821 aagctattta tcagcaagcg cgttgtctgc tacataaaga tgcgattacc gcgctggcga
     5881 aaagtatcag tatcgcccag ctgtcaggct tacaactggt gatttacgat gcgtatcgcc
     5941 cacaacaagc acaggcgatg ttgtggcaag cctgcccaga cccgcaatat gttgttgatg
     6001 tgacggtcgg ttctaatcac agccgtggca cggcgctcga cctgacgctt cgtgatgacg
     6061 tggaggtggt gaatgatgct aagcgaggaa acgtccgccg tacgcccaca aaaacaaacg
     6121 cgatttaacg gtgcaaaact ggtatggatg ctgaaaggca gtccgctcac cgtgaccggc
     6181 gcagtcatca ttgtattaat gcgactgatg atgatttttt caccgtggct ggcgacgcat
     6241 gatcccaacg ccattgattt aaccgcccgc cttttgccgc cttctgcggc gcactggttt
     6301 ggcaccgatg aagtgggacg cgatctgttt agccgcgtac tggtcggcag tcagcaatca
     6361 attctcgccg gattagtggt ggtcgccatt gcgggtatga ttggttcgct actcggatgt
     6421 ctatccggtg tgcttggcgg acgcgcagac gccatcatca tgcgcatcat ggacattatg
     6481 ctgtcgattc cttcgttggt actgacaatg gcactggctg ccgctctcgg gccgagttta
     6541 tttaacgcca tgctggcgat tgctattgtg cgaattccct tttatgtgcg cctggcgcgg
     6601 gggcaagcat tagttgtacg ccagtatacc tatgttcagg cggcgaaaac ctttggcgcg
     6661 tctcgttggc atctgatcaa ctggcatatt ttacgtaact ccctaccgcc gctgatcgtg
     6721 caggcatcgc tggatatcgg tagcgcgatt ttaatggccg ccacgttggg atttattggc
     6781 ctgggtgctc aacaaccgag tgctgaatgg ggcgcgatgg tagcgaatgg tcgcaactat
     6841 gtactcgatc agtggtggta ttgcgcattt ccgggggcag cgattttgct taccgccgtc
     6901 gggtttaatc tctttggtga tggtattcgc gatctgcttg acccgaaagc aggaggaaag
     6961 cagtcatgac ccaacccgtt ctggacattc aacagctgca tttgagtttc cccggtttta
     7021 acggtgatgt tcacgcgctc aacaatgtgt ccttgcagat taaccgcggc gaaattgtcg
     7081 gtctggtggg agaatccggc tcaggtaaat cagtcaccgc aatgctgatt atgcgtctgc
     7141 taccgacggg cagttattgc gtacatcggg ggcagatttc actgctggga gaagatgttc
     7201 ttaacgctcg ggaaaaacag cttcgtcagt ggcgcggcgc acgagtggcg atgatctttc
     7261 aggaaccgat gaccgccctt aatccgacac gtcgaatagg tcttcagatg atggacgtaa
     7321 tccgccatca tcaaccaata agtcgtcggg aagccagagc taaagcgatt gccctgctgg
     7381 aagagatgca aatcccggat gccgtggaag ttatgtcgcg ctatccgttt gagctttcag
     7441 gtggtatgcg ccagcgggta atgattgcgc tggcattctc ctgcgagccg caattgatta
     7501 ttgccgacga accgacgacg gcgctggacg tcacagtaca gttgcaggta ctgcgtttgc
     7561 ttaaacataa agcacgcgcc agtggaactg cggtactgtt catcagccat gatatggccg
     7621 tggtgtcgca actgtgcgat agcgtttacg tgatgtatgc cggaagcgta attgaaagcg
     7681 gcgggacggc agacgttatc catcatcccc ggcatccgta taccattggt ttgctgcaat
     7741 gcgcaccaga acatggagta ccacgccagc cattacccgc cattccaggg acggtaccaa
     7801 acctcaccca tttgcctgac ggctgcgctt ttcgcgatcg ttgctatgcg gcaggtgcac
     7861 agtgtgaaaa cgtcccggcg ctgacagcgt gtggtgacaa caaccagcac tgcgcctgtt
     7921 ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg cgacgtccat
     7981 atcaattttc cggcccgtaa aaactggctt ggtaaatcta cggaacatgt tcatgccatt
     8041 aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg cgagtcaggc
     8101 tgcggcaaaa gcaccctcgc acagctttta atgggcatgc tgcaaccgag ccacgggcag
     8161 tacatccgtt caggctcaca acgcattatg cagatggtgt ttcaggaccc gctctcttcg
     8221 cttaatccgc gcttaccggt gtggcgcatc atcacagaat cgctctggat agctaagcgt
     8281 agtagtgaac aacagcggcg agcgttggca gaggagttgg ctgtgcaggt gggtattcgt
     8341 ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca acgcatcgcc
     8401 attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc aacctcggcg
     8461 ctggatatct ccgtgcaggc gcagatcctc aatttactgg taacgctaca ggaaaatcac
     8521 gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat gagcgatcgg
     8581 gtggcggtga tgtatctcgg gcagattgta gaactgggcg acgcgcaaca ggtgttgact
     8641 gcttcggcac atccatacac ccgattattg ctggattccc tccccgccat tgataaaccg
     8701 ctggaggaag aatgagcatt acgtaaaacg gagctgccgg gaaaccgcac gttgccgcaa
     8761 ggctgttttt tctacgaacg ttgccctctg gcaacccacg gatgtgaagt ccggcaatca
     8821 ttagcaataa gggaggacgg acgtgagctc cggtgctggc gggcgctgta gagatgctaa
     8881 caacgcatca ggcattcacg cgcctgacgc gtcatccggc aatgctttac gatttcaact
     8941 ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct gcttttgctt
     9001 tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact tcactcttca
     9061 gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca tcggcggtgg
     9121 tatcaattga tgttggcgtg aatcccgctt cccccagcat taatgaaagc gccattgaga
     9181 aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt tcgccttcaa
     9241 aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc gctctcggtg gatactgttc
     9301 ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg attgtcattg
     9361 ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag cacgaggata
     9421 agcaggatat tccgaataaa atacggcgaa aatattactt ctgcacttaa attaaattcc
     9481 cacaatagat aaccgggaat atatatctca a
//
LOCUS       DAJTRQ010000138         9383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000138 DAJTRQ010000000
VERSION     DAJTRQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(437..643)
                     /locus_tag="OO332_003490"
     CDS             complement(437..643)
                     /locus_tag="OO332_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5943830.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="OO332_003491"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="OO332_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5943831.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="OO332_003492"
                     /pseudo
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="OO332_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="OO332_003493"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="OO332_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="OO332_003494"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="OO332_003494"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5943832.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..6345
                     /locus_tag="OO332_003495"
                     /pseudo
     CDS             5362..6345
                     /locus_tag="OO332_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6345..8041
                     /locus_tag="OO332_003496"
                     /pseudo
     CDS             6345..8041
                     /locus_tag="OO332_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            8038..8715
                     /gene="kdpE"
                     /locus_tag="OO332_003497"
     CDS             8038..8715
                     /gene="kdpE"
                     /locus_tag="OO332_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5943833.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8990..9094
                     /locus_tag="OO332_003498"
     CDS             8990..9094
                     /locus_tag="OO332_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5943834.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT         2075 a         2372 c         2609 g         2327 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aaacatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttacc actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc tactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcattt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccatag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttaatcg cactgttttt tattcaacaa
     1621 ggtgcgctgc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatac ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgctccgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctcc ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcaccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggtggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggcta ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaacgtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcgcggcg gcgcggctgg
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga accccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcag aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggcgcta ttttatggac gctggccttc ggtggttgcc accgtcatta
     6721 atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg
     6781 tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta
     6841 ctgctggcgt gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc acacggcact
     6901 tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca
     6961 gcgaacaatt tattgcctct acgtttcatg cccgcagtca ggtgttgttg cccgatgaca
     7021 acggtaaatt gcagccgtta acacatccgc aaggaatgac gccatgggac gatgccatcg
     7081 cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg ttacccggtg
     7141 taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacggctgg tggtggtgga
     7201 gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacttttac
     7261 gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg
     7321 gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt
     7381 acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag
     7441 cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac
     7501 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
     7561 gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg
     7621 tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
     7681 gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc
     7741 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
     7801 ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg
     7861 cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat
     7921 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
     7981 tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg
     8041 acaaacgttc tgattgttga agatgaacag gctattcgta gctttctgcg cacggcgctg
     8101 gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg
     8161 gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
     8221 gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc
     8281 agcgaagaga gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag
     8341 ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc
     8401 accgccgcgc ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc
     8461 gtgattcacc ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctagcg
     8521 gtgctgctca acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg
     8581 gggccaaacg cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa
     8641 aaactggaac aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattgggtat
     8701 cggtttatgc tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt
     8761 aaacctgcgc cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac
     8821 gaaattacaa catttaagta acaaaacagc atctaatatg gcgatattca taattaatga
     8881 tttattatct ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca
     8941 tggatagaat aactctacct tcaactgaca cagcaagcgg taaaggtaaa tggaaaataa
     9001 taaccgctta atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa
     9061 tagttttgac tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg
     9121 tacaggtctc cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat
     9181 actgctaacc cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg
     9241 acgagtatag ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact
     9301 gatttattta tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga
     9361 gaaattgagg acctgctatt acc
//
LOCUS       DAJTRQ010000139         9133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000139 DAJTRQ010000000
VERSION     DAJTRQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            373..1491
                     /locus_tag="OO332_003499"
                     /pseudo
     CDS             373..1491
                     /locus_tag="OO332_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1557..1805
                     /locus_tag="OO332_003500"
     CDS             1557..1805
                     /locus_tag="OO332_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5943835.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1870..2238
                     /locus_tag="OO332_003501"
     CDS             1870..2238
                     /locus_tag="OO332_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5943836.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2332..2985
                     /gene="nfsB"
                     /locus_tag="OO332_003502"
     CDS             2332..2985
                     /gene="nfsB"
                     /locus_tag="OO332_003502"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5943837.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3093..4340
                     /gene="ybdG"
                     /locus_tag="OO332_003503"
     CDS             3093..4340
                     /gene="ybdG"
                     /locus_tag="OO332_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5943838.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4408..5784)
                     /gene="pheP"
                     /locus_tag="OO332_003504"
     CDS             complement(4408..5784)
                     /gene="pheP"
                     /locus_tag="OO332_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5943839.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5885..9020)
                     /gene="cusA"
                     /locus_tag="OO332_003505"
                     /pseudo
     CDS             complement(5885..9020)
                     /gene="cusA"
                     /locus_tag="OO332_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
BASE COUNT         2251 a         2534 c         2195 g         2153 t
ORIGIN      
        1 atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg gaaagcttca
       61 ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat acagcaaatc
      121 ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt tttttctgca
      181 tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg ccacattgaa
      241 aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa agaaacattc
      301 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgtcaact
      361 taagagaaac ctatgccatt acccgatttt catttttctg aaccttttac cctcggtatt
      421 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
      481 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
      541 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcagttt
      601 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
      661 ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa cgaacgctat
      721 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
      781 gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
      841 tttgtgccgc attttattgc cctttccgcc gcgtcgccat atatgcaggg aacggatacg
      901 cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg cccgatgccg
      961 tgggtcagta actagcaaca atttgaagcc ctgtttcgct gtctgagtta caccacgatg
     1021 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
     1081 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtgaatat ggcgggatta
     1141 attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca ggagaaagat
     1201 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga aggcgtcatt
     1261 accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg attgctggaa
     1321 aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc actgcatcgc
     1381 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggcggc
     1441 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta agccgggaat
     1501 tgccttacag aagaatatcc ctgacaatgg tgttttaact tatatttaac aatgttatga
     1561 aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct ttcctctctt
     1621 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
     1681 tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg tttttagtgc
     1741 tgagcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
     1801 attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc ttcgcctaaa
     1861 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc tcttgagtta
     1921 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
     1981 aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct gaagtccgat
     2041 ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccaag gtatcacatg
     2101 aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc gttacttcgc
     2161 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
     2221 agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta ccatttctcg
     2281 ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt tatggatatc
     2341 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa aaaacttacc
     2401 ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag caccaactcc
     2461 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
     2521 gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca cgtcgtggtg
     2581 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
     2641 gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
     2701 ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
     2761 tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga cgcggtgccc
     2821 atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga gaaaggctac
     2881 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
     2941 ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc tcttgccggg
     3001 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
     3061 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
     3121 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
     3181 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
     3241 aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat aaactcttcc
     3301 accgtttagc ttttaccctg cagggaatta tcgtcaatat tcaggcggta ttctggctgc
     3361 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
     3421 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
     3481 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcaa
     3541 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
     3601 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
     3661 tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaaa
     3721 tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
     3781 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
     3841 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
     3901 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
     3961 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
     4021 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
     4081 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
     4141 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
     4201 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
     4261 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
     4321 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
     4381 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
     4441 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
     4501 catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga acggatagag
     4561 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg
     4621 cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg tcatcagcag
     4681 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
     4741 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
     4801 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
     4861 atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta ccacattgct
     4921 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
     4981 atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc gatacaccac
     5041 ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg ctgtaatccc
     5101 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
     5161 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
     5221 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga tcgccagcac
     5281 tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
     5341 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
     5401 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
     5461 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
     5521 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
     5581 gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg ccatctgaat
     5641 cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
     5701 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
     5761 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
     5821 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cgaggtatcc
     5881 tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc gccgggataa
     5941 taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggggcg gcaatccggc
     6001 tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg gcgataatca
     6061 ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac aacgcctcat
     6121 ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc tctatggcgt
     6181 gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca agggcaataa
     6241 agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac cagatgccgc
     6301 ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc cgacggaatg
     6361 ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt ttcagcttat
     6421 ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg cccggtttta
     6481 actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc atgtcacgat
     6541 cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc agcatcgacg
     6601 gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc ttcatcggcg
     6661 tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc ggataacgca
     6721 gattaattgg ataacgggca atcccttcca ccctttcgcc aaccatcgcc ccgcccaccg
     6781 cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg gctttttcac
     6841 ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc gcagaagcta
     6901 cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg atatccgcca
     6961 gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag agcatatcga
     7021 tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc acggtgttat
     7081 ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc tgtggcttaa
     7141 gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt tccgctttcc
     7201 cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg tcggtttttt
     7261 gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc atatacaaca
     7321 agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc ggccagagaa
     7381 ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc agtactttca
     7441 gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg ctttccggcg
     7501 gaattttgcc acggatccag taacccatca ggatagggat cactacgatc gccagcagcg
     7561 ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga cgcccttcct
     7621 gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga ctgataaaca
     7681 gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt ttattatcca
     7741 gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca ttctcgatca
     7801 tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc agcgacataa
     7861 tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac cccagcggca
     7921 acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg cagactaccg
     7981 ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc cggtcaatga
     8041 gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta cttttcagcg
     8101 tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg gatcgcagga
     8161 tcaccacccc gcccgccact tcgccttcgc cgtttaatgc cccggcgcat ctccgggcca
     8221 atctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact ggcttttaaa
     8281 acgatgtgat taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac catatattct
     8341 gcttccgcca gttcgattga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt
     8401 tttacttcgg cgagactgat gccatactgc gccaggcgct gaggatcgat aaccacctga
     8461 tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat ggttttcagc
     8521 tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg cttaccgctg
     8581 cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg tcccagctcg
     8641 gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgaa
     8701 cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac
     8761 tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga
     8821 taagtcacct gattttcaac gatttgcggt gcctggccgg gatagctggt tttaataatc
     8881 acctgcacat cggagagatc cggcaacgca tccactggcg tattaatgat ggtccaggtg
     8941 ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc caccgagcga
     9001 cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc
     9061 gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac aggccgccgg
     9121 aaaccacctt ttc
//
LOCUS       DAJTRQ010000140         9057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000140 DAJTRQ010000000
VERSION     DAJTRQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(330..674)
                     /locus_tag="OO332_003506"
     CDS             complement(330..674)
                     /locus_tag="OO332_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5943840.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            806..2716
                     /locus_tag="OO332_003507"
     CDS             806..2716
                     /locus_tag="OO332_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5943841.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2774..3469
                     /gene="tsaB"
                     /locus_tag="OO332_003508"
     CDS             2774..3469
                     /gene="tsaB"
                     /locus_tag="OO332_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5943842.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3509..4090
                     /gene="yeaY"
                     /locus_tag="OO332_003509"
     CDS             3509..4090
                     /gene="yeaY"
                     /locus_tag="OO332_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5943843.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4295..5980
                     /gene="fadD"
                     /locus_tag="OO332_003510"
     CDS             4295..5980
                     /gene="fadD"
                     /locus_tag="OO332_003510"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5943844.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6062..7177
                     /gene="rnd"
                     /locus_tag="OO332_003511"
     CDS             6062..7177
                     /gene="rnd"
                     /locus_tag="OO332_003511"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5943845.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7231..8196)
                     /gene="yeaX"
                     /locus_tag="OO332_003512"
     CDS             complement(7231..8196)
                     /gene="yeaX"
                     /locus_tag="OO332_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5943846.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8252..8869)
                     /gene="yeaW"
                     /locus_tag="OO332_003513"
                     /pseudo
     CDS             complement(8252..8869)
                     /gene="yeaW"
                     /locus_tag="OO332_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         2188 a         2239 c         2434 g         2196 t
ORIGIN      
        1 gagttaacct gttgacattc aggtgtttta aaggtagcag ctcatggtgt gttatccagt
       61 aagatggaga aaattaacgg tcagcccgca cgctataacc agattgttca gctaagaaag
      121 aaaaccagag ttgctaatca aagggcgaac acaacggcat ggttatgttt atttatatag
      181 ttagttttat ggataaaatt gaaattataa atataaataa tgaattagca gaagcatcaa
      241 cagtgatatc ttattctctg gtcgatttat gtaaaaccga ccagtattat cgataaatta
      301 tcgtctgact tattttgctt cgataaagct tacaccgcag ccacaatctt aatttcaact
      361 ttatacttcg ggttcatcaa acccgcttgt accgtgcagc gcaccggcgc atgacccgcg
      421 acaacccagg catcccacgc tttattcatc gccgcgaagt cgtttttatc ggccaggaaa
      481 atggtggcat ccagaatgct cgatttattg ctgccctgtt tttccagcac ggcgtcaatc
      541 tgtgccagcg tgttggcggt ttgctcaaag gcatcggcgt cgaggttttc cggtacacca
      601 gtgtagtaga gcgtgttgtt gtggattact acatcagacc agcgggcttc agcatcgata
      661 cgaacgatag tcatcatttt gtcctcatta tacttccatt ttcgtgctgc aaagactgcc
      721 acaatgtgag gttgccgtca ccacttcaac tggcgaaagc gccctcatcc tgacataatc
      781 cctgttcaaa tcaacagggg gtagtgtgac ggacgatttt gcaccagacg gtcagctggc
      841 gaaagcgata ccaggcttta agccgcgaga accacagcga cagatggcgg tagccgtcac
      901 ccaggcgata gaaaaaggcc agccgctggt ggtggaagca ggaaccggta cgggcaaaac
      961 ctacgcttac ctggctcctg cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc
     1021 aaaagcgttg caggatcagc tctacagccg cgatttgcca acagtctcaa aggcattgaa
     1081 atatacgggc aacgtggcgc tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct
     1141 cgaacagcag gcgctggcgg ggggcgatct gccggtacaa atcttaagcg atgtgatcct
     1201 gctgcgctcc tggtctaatc aaacagtcga tggtgatatc agcacctgcg tcagcgtggc
     1261 ggaagattca caggcgtggc cgctggtcac cagcaccaac gacaactgtc ttggcagcga
     1321 ctgcccgatg tataaagatt gctttgtggt caaagcacgt aaaaaagcga tggacgccga
     1381 tgtggtggtg gtaaaccatc atctctttct ggcggatatg gtggttaaag agagtggatt
     1441 tggcgagttg atccctgaag cggacgtcat gatcttcgac gaagcccacc agctaccgga
     1501 cattgccagc cagtattttg gtcagtcact ctccagtcga caactgctcg acctggcaaa
     1561 agacatcacc atcgcctacc gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc
     1621 tgatcgtctt gcccagagtg cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg
     1681 cggtaacctg cgtgagctgt tagctaatcc gcaaattcag cgggcatttt tactgctcga
     1741 tgacaccttg gaactttgtt atgacgtggc gaaactgtca ctggggcgtt ccgccttgct
     1801 ggatgcggca tttgagcgcg ccacgttgta tcgcacacgg ctgaagcggc taaaagagat
     1861 caatcagccg ggctacagct actggtacga atgcacttcg cgccatttta ctctggctct
     1921 cacgccgctc agcgtggcgg ataaattcaa agagttaatg gcgcaaaagc ccggtagctg
     1981 gatcttcacc tcagcaacgc tgtcggtgaa cgacgatctg catcatttca cctcgcggct
     2041 tgggatagaa caggcggagt cgttgctatt gccaagcccg tttgattaca gccgccaggc
     2101 gttactctgt gtgccgcgca atctgccgca aaccaaccag ctaggttctg ctcgccagtt
     2161 agcggcaatg ctgcgaccga tcatcgaagc taacaacggt cgttgtttta tgctttgtac
     2221 ctcgcacgcc atgatgcgcg atctggccga gcagttccgc gctaccatga cgcttcccgt
     2281 tttgttgcag ggggaaacca gtaaaggcca actgttgcag cagtttgtca gtgccggtaa
     2341 tgcgcttctt gtggcaacca gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt
     2401 gtcattggta attatcgaca aattgccgtt tacctcgctg gatgatccac tgttaaaagc
     2461 gcgcatggaa gattgtcgtt tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga
     2521 tgccgtcatt actctcaaac agggggtagg tcgactgatt cgcgacgccg acgatcgtgg
     2581 cgtgctggtg atttgtgact atcggctggt gatgcgtcct tacggcgcga cgtttctcgc
     2641 cagtctgccg cccgcgccac gcacccgtga cattgcccga gcggttcgtt tccttgcgat
     2701 accatcctcc aggtaatttg ttacggagta tggtatgatg cgcgcctttt tattgatcta
     2761 agcacgaaga cccatgcgaa ttctggctat cgataccgcg acagaggcct gctctgtcgc
     2821 cctgtggaac gacggtactg tcaacgctca ttttgagctt tgccctcgtg aacatactca
     2881 acgaatctta ccgatggtgc aggatatcct gaccaccagc ggaacttccc tgactgatat
     2941 taacgctctg gcttacgggc gcggccccgg tagctttacc ggcgtgcgca ttggtattgg
     3001 catcgcgcaa gggctggcgc ttggcgcgga attaccgatg attggcgtct ccacgctaat
     3061 gacgatggcg caaggggcgt ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga
     3121 cgcgcgaatg ggcgaagttt actgggccga atatcagcgt gatgaaaacg gtatctggca
     3181 cggtgaagaa accgaagccg tactcaaacc cgaactcgtt catgaacgaa tgcaacagct
     3241 ttccggcgaa tgggtgacgg tagggacggg ctggcaagcc tggccggatc tcggtaaaga
     3301 gagcgggctg gttttgcgcg atggcgaagt gttactcccc gctgcagaag atatgctgcc
     3361 gattgcttgt cagatgtttg ccgagggtaa aacggtggcg gtggaacatg ccgagccggt
     3421 ttatttgcgt aacaacgtcg catggaagaa acttccgggc aaagaatgaa tcttaagagt
     3481 atgccctgag aaaaaaagga gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat
     3541 actggcaggt acgtttgcgc taatgctgag tggttgtgtc actgtgccgg acgccattaa
     3601 aggcagcagt cccacgccgc aacaagattt agttcgggtg atgagtgcgc cgcagctgta
     3661 cgttggtcag gaggcacgct ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa
     3721 aacccgcctg gaaattgcta ccgtaccgct ggacagcgga gctaggccga cgctgggaga
     3781 accttctcgc ggtcgcattt atgccgatgt gaacggtttt ctggacccgg tggatttccg
     3841 tagacaactg gttacggtag tcgggccaat caccggtgcg gttgacggca aaatcggcaa
     3901 tacgccctat aaatttatgg tgatgcaagt aacgggttac aaacgttggc atttaaccca
     3961 gcaggtgatt atgccgcctc agccgattga tccatggttt tatggcggtc gtggctggcc
     4021 ctatggctac ggcggatggg gctggtataa tcccggcccc gcgagagtac aaacagttgt
     4081 aaccgaataa ttgcttgttt ttaaagaaaa agaaacagcg gctggtccgc tgtttctgca
     4141 ttcttacggt aaagataaaa ataaatagtg acgcgcttcg caaccttttc gttgggtaat
     4201 tatcaagctg gtatgatgag ttaatattat gttaacagcg tgtatatcat ttggggttgc
     4261 gatgacgacg aacacgcatt ttagaggtga agaattgaag aaggtttggc ttaaccgtta
     4321 tcccgcggac gttccgacgg agatcaaccc tgaccgttat caatctctgg tagatatgtt
     4381 tgagcagtcg gtcgcgcgct acgccgatca gcctgcgttt gtgaatatgg gggaggtgat
     4441 gaccttccgc aagctggaag aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt
     4501 ggggctgaag aaaggcgatc gcgttgcgtt gatgatgcct aacttattgc aatatccggt
     4561 ggcgctattt ggcattttgc gtgccgggat aatcgtcgta aacgttaacc cgttgtatac
     4621 cccgcgtgag cttgagcatc agcttaacga tagcggcgca tcggcgattg ttatcgtgtc
     4681 taactttgcc cacactctgg aaaaagtggt tgataaaacc gccgttcagc acgtaattct
     4741 gacccgtatg ggcgatcagc tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa
     4801 atacatcaag cgcttggtgc cgaaatacca tctgccagat gccatttcat ttcgtagcgc
     4861 actgcataac ggctaccgga tgcagtacgt caaacccgaa ctggtgccgg aagatttagc
     4921 cttcctgcaa tacaccggcg gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg
     4981 caatatgctg gcgaacctgg aacaggtcaa cgccacctat gggccgttgc tgcatccagg
     5041 taaagaactg gtagtaacgg cactgccgct gtatcacatt tttgccctga ccattaactg
     5101 cctgctgttt atcgaactgg gtgggcagaa cctgcttatc actaacccgc gtgatattcc
     5161 agggttggta aaagagttag cgaaatatcc gtttaccgct atcactggcg ttaacacctt
     5221 gttcaatgcg ttgctgaaca ataaagagtt ccagcagctg gatttctcca gtctgcatct
     5281 ttccgcaggc ggtgggatgc ctgttcagca agtggtggca gaacgctggg tgaaactgac
     5341 cggacagtat ctgctggaag gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa
     5401 cccgtatgat attgattatc atagcggtag catcggtttg ccggtgccat cgacggaagc
     5461 caaactggtg gatgacgacg acaacgaagt accaccgggt caaccgggtg agctttgtgt
     5521 caaaggaccg caggtgatgc tgggttactg gcagcgtccg gatgctacag atgagatcat
     5581 caaaaatggc tggttacaca ccggcgacat cgcggtgatg gatgaagaag gattcctgcg
     5641 cattgtcgat cgtaaaaaag acatgattct ggtttccggt tttaacgtct atcccaacga
     5701 gattgaagat gtcgtcatgc agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc
     5761 ttccggctcc agtggtgaag cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac
     5821 cgaagagtca ctggtgactt tttgccgccg tcagctcacg ggatacaaag taccgaagct
     5881 ggtggagttt cgtgatgagt taccgaaatc taacgtcgga aaaattttgc gacgagaatt
     5941 acgtgacgaa gcgcgcggca aagtggacaa taaagcctga gcgttaagtc agtcgtcaga
     6001 cgccggttaa tccggcgttt tttttgacgc ccactaaaga gaaaacaatt tgaattacca
     6061 aatgattact acggacgatg cgctggcttc tttgtgtgaa gccgtccgtg cctttccggc
     6121 gatagccctg gatactgaat ttgttcgtac gcgcacttat tacccgcagc tggggttgat
     6181 tcaacttttc gatggcgagc atctggcgct aattgatcct ctcgggatca ccgactggtc
     6241 accgctgaaa gcgatcctgc gcgatccgtc catcacaaaa tttctccatg caggcagtga
     6301 agatctggaa gtgttcctca atgtctttgg cgaattacca caacccttga ttgacacgca
     6361 aatccttgct gccttctgcg gacgcccgat gtcatggggt ttcgcttcca tggtggaaga
     6421 gtattccggc gttacgctgg acaagagtga atcgcgcgcc gactggctgg ccagaccgct
     6481 gaccgaacgt cagtgtgaat acgcagcggc ggatgtctgg tatctgttac cgatcactgc
     6541 caagcttatg gtagaaacgg aggcctccgg ctggctacct gcggcgctgg atgaatgccg
     6601 cctgatgcaa atgcgtcgtc aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac
     6661 caatgccagg cagttacgca cacgccaact ggcctgtctg caactgttag ccgactggcg
     6721 actgcgcaag gcgcgagagt gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt
     6781 gtggtcggtc gcgcgttata tgccgggaag tttaggcgaa ctggacagcc tgggtttatc
     6841 cggtagcgaa atccgctttc acggtaaaac gctgctggcg ctggtggaaa aagcgcagac
     6901 attgccggaa gatgccttac cgcagccgat gtttaacctg atggacatgc ctggttatcg
     6961 taaagcgttt aaagcgatta agtcgctgat tactgacgtg agcgaaacgc ataagatcag
     7021 cgccgaattg ctggcatcgc gtcggcaaat caaccaactg ctgaactggc accggaaact
     7081 gaaaccgcag aacaatttgc cggagctgat ttccggctgg cgtggtgagc tgatggcgga
     7141 agcattacac aatttattgc aggaatatcc gcagtaaaat cttccgaagc cggactgggc
     7201 gcgctcagcg ccacatccgg ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt
     7261 cgcacgcgaa caacagatca acatactttg ctggctggca cgctcttcat cgctaaaata
     7321 ctgatcccga tggtcagctt caccttccag tattgccgtt tcgcaggttc cgcatacccc
     7381 ttcacgacat aaacattcca ctttcgcggc tttattattt tcaataacct gcaaaatagt
     7441 catctcttcc ggcaccacaa actcttttcc ggaacgggca agcaccaggg taaatgcatc
     7501 gccggttttg tcttcgatag caaattgctc aaagtgcagc gtatcggcgg cgatgtccag
     7561 acgcgcagct tcacttctta ccgcttcaat tagcgcctcg gggccacagg tataaacgtg
     7621 tgtgccaggt tcgatatccg ccaataatcg cgctaattcc agtcgtgttc cggttgatga
     7681 atgatgcaaa tggactttct cagcctgcgg atgctggact agctcatcac gaaatgcgca
     7741 actttctgga tttcgcgagc agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc
     7801 tgccatgtgc gacaggaaag gggtgatacc aataccgccc gcgataaaca gatgctttct
     7861 ggctgaggga attagcgcaa acaggttatt aggcgttgag atcgttaacc gatcgcccac
     7921 ttttacctgc tgatgcaaaa agcgggaacc gccgcgcgag ttttcttcca gtcgaactgc
     7981 aatctgataa ctagaggtgt tatgcggtga actcagtagt gaatacgcat tgctgtactg
     8041 gttatcacca tcgctcatct gcactaggat gtgacttcct ccggtgaacg caggcagcgg
     8101 tttgccatcg gttgccacca gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg
     8161 gctcacctgt acttcaaaca tttgatagtc tgacattgct gtactccatc aaaaatcagc
     8221 ccagcggcgg aaaataccgc cgccgatgtc attagtcctt aaacacctgc gccagcagat
     8281 tatggaaatg ggcgatacca tgttcggaaa tgccactgcc gctactgtcg gccatgatgc
     8341 gcccctgacc acgatagcca cgcgatttca gccctttctg tacgctttca accagacgta
     8401 aatcttccgg atggaacaca tcgcgatacc actcaatcag cgatttttgc tcgttggtta
     8461 actcttcatt ggtgaagtaa atatcgtagt tttgcagggt agtttcagaa tccaccggga
     8521 attcataaat gaccgtcatc atccctttga tcggggtgac gttcagcatc gtgcacggcc
     8581 acagccagaa accgtggaat gccgcatccg taccctcttc aaatttaaac gactgttcgg
     8641 acggtttggc gaaaccgtat tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat
     8701 caacctgtac ggagtcggag aaacctagat gcgccggacc acagtgatag cactcgagat
     8761 agttatcgac aatgttcttc cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt
     8821 tcagattgtg gacttccggg caggcttcca gcactttcgc gcccagaccg gtgtattgat
     8881 tcacttgaag tacgaaaaaa aaccgggagg acattggatt attcgggttc tgatgggatt
     8941 agatttggtg gggcttgcaa gcctgtagcg caaattttag tcgttaatca atgaaacgcg
     9001 aaagatagta aaaaattgct tttgtttcat tgaaaatacg aaaaacaaaa acactgc
//
LOCUS       DAJTRQ010000141         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000141 DAJTRQ010000000
VERSION     DAJTRQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO332_003514"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO332_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5943847.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO332_003515"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO332_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO332_003516"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO332_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5943848.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="OO332_003517"
     CDS             complement(2403..2606)
                     /locus_tag="OO332_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943849.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO332_003518"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO332_003518"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5943850.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO332_003519"
     CDS             complement(3802..4014)
                     /locus_tag="OO332_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5943851.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO332_003520"
     CDS             4274..4582
                     /locus_tag="OO332_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5943852.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO332_003521"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO332_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5943853.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO332_003522"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO332_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5943854.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO332_003523"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO332_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5943855.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO332_003524"
     CDS             complement(8478..8981)
                     /locus_tag="OO332_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5943856.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2235 a         2314 c         2148 g         2332 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcagcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattat
//
LOCUS       DAJTRQ010000142         9000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000142 DAJTRQ010000000
VERSION     DAJTRQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(85..1461)
                     /gene="trkA"
                     /locus_tag="OO332_003525"
     CDS             complement(85..1461)
                     /gene="trkA"
                     /locus_tag="OO332_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5943857.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1483..2772)
                     /gene="rsmB"
                     /locus_tag="OO332_003526"
     CDS             complement(1483..2772)
                     /gene="rsmB"
                     /locus_tag="OO332_003526"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5943858.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2818..3765)
                     /gene="fmt"
                     /locus_tag="OO332_003527"
     CDS             complement(2818..3765)
                     /gene="fmt"
                     /locus_tag="OO332_003527"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5943859.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3780..4289)
                     /gene="def"
                     /locus_tag="OO332_003528"
     CDS             complement(3780..4289)
                     /gene="def"
                     /locus_tag="OO332_003528"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5943860.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4437..5561
                     /gene="dprA"
                     /locus_tag="OO332_003529"
     CDS             4437..5561
                     /gene="dprA"
                     /locus_tag="OO332_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5943861.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5533..6006
                     /locus_tag="OO332_003530"
     CDS             5533..6006
                     /locus_tag="OO332_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5943862.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6035..6577
                     /locus_tag="OO332_003531"
     CDS             6035..6577
                     /locus_tag="OO332_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5943863.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6582..7154
                     /gene="tsaC"
                     /locus_tag="OO332_003532"
     CDS             6582..7154
                     /gene="tsaC"
                     /locus_tag="OO332_003532"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5943864.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7159..7977
                     /gene="aroE"
                     /locus_tag="OO332_003533"
     CDS             7159..7977
                     /gene="aroE"
                     /locus_tag="OO332_003533"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5943865.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7974..8231
                     /locus_tag="OO332_003534"
     CDS             7974..8231
                     /locus_tag="OO332_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5943866.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8207..8761)
                     /locus_tag="OO332_003535"
     CDS             complement(8207..8761)
                     /locus_tag="OO332_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5943867.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2250 a         2188 c         2247 g         2315 t
ORIGIN      
        1 aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag ggaaataata
       61 aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga agagtctttc
      121 gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc cttgctcaat
      181 gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc caataatcgt
      241 tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc
      301 accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg aaacaccaac
      361 aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat
      421 tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct ggatcaatac
      481 catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat tggcctcgtc
      541 atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg ccagcagttc
      601 ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg ccagttcggc
      661 agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca gacgacgcgc
      721 cagccctgca ccgatattac cgccaccaac cagcatgatc cgtttatacg gtttttccag
      781 tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa agaacacttc
      841 atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt ggcggaaaat
      901 tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa gtgcattacc
      961 aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac cctcagcgaa
     1021 gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat cgataaccaa
     1081 ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata gcttatcggc
     1141 atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt tgaaaagtga
     1201 gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag caaccagcat
     1261 atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat gcccctgcac
     1321 gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg tatcgacaac
     1381 agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa cctggccggc
     1441 acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt tgattagctt
     1501 agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac ccggttgctc
     1561 tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg
     1621 caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc caccggattt
     1681 taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt ccgggatatc
     1741 gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg ttgctgaaca
     1801 aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg aagggtaacg
     1861 gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt cgtaaacgcg
     1921 agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg ccacctcaag
     1981 gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt caccgttttg
     2041 tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa cccatccgtc
     2101 ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag catcagggta
     2161 atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc agctgtcgcg
     2221 ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat tggcttcggc
     2281 gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc aggaaggatg
     2341 cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc gacgctggaa
     2401 ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg cgatagcgcc
     2461 ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca gttgatacaa
     2521 accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac gggccattaa
     2581 cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc acaactcttg
     2641 aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa tgttgcttaa
     2701 tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac gtaaattacg
     2761 ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag tggactatca
     2821 gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt cttgcgcgct
     2881 catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat caccagtcgc
     2941 aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt tggttgccgt
     3001 atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca gccagctcat
     3061 tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa gtgaccagtc
     3121 aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt cgtcctgaac
     3181 ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc cttgtgggcc
     3241 aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcagga
     3301 gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa tggttacacc
     3361 agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg
     3421 cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca cagctttagg
     3481 cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg cgaccagttg
     3541 ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt cttcctcagc
     3601 cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg
     3661 ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc
     3721 aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag ttcttatcct
     3781 taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg ttgttgtttc
     3841 agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc atgctgaata
     3901 cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg gtcaagggcg
     3961 cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat cgacaggcaa
     4021 ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat taacaccagc
     4081 cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat atcaacctgg
     4141 gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata
     4201 cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc
     4261 ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa
     4321 agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac ttgattgaca
     4381 gcatcactga ccaatcgcaa agattgctaa ggcttgctta tgacagggag ataaggatgg
     4441 tcgatacaga aatttggctg cgtttaatga gcatcagcag cttgtacggc gatgatatgg
     4501 tccgtatcgc tcactggctg gcaaaacagt cgcatattga tgcggttgta ttgcagcaaa
     4561 cagggcttac attgcggcag gcacaacgct ttctttcatt tccacgaaag agtatcgaaa
     4621 gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac agcgaatttt
     4681 atcctcctca acttcaggcg acgacagatt accccggcgc actgtttgtt gaaggagaac
     4741 tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat tcatggtatg
     4801 gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac gtgtggagtg acaattacga
     4861 gtggactggc gcgtggaatc gatggtgtgg tgcataaagc ggccttacag gtaaatggcg
     4921 tcagcattgc tgtattgggg aatggactta atatcattca tccgcgtcgc catgcccgac
     4981 tggctgccag tctgtttgaa cagggtggcg ctctcgtctc ggaatttccc ctcgatgttc
     5041 caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta agtaaaggtg
     5101 tactggtggt ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt tgtgcgcttg
     5161 agcaggggcg tgaagttttt gccttgccag gtccaatagg gaatccggga agcgaagggc
     5221 ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa attctggaaa
     5281 acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt tattcaccag
     5341 atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga gatgaggtaa
     5401 cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta gttactcaac
     5461 tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat gtccgattga
     5521 ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat tcacactgaa
     5581 gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc aggttttgag
     5641 cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta tcaggaaggg
     5701 ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta tacaccggaa
     5761 gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga gcagattcag
     5821 gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct ggataacgca
     5881 gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa catcccgggc
     5941 tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga aggtatgctg
     6001 cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac tgttcacggt
     6061 gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat ccgggaaaca
     6121 cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc ctctgaaatc
     6181 ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccccg tatgtggcgc
     6241 aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta actaccctga
     6301 atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat gcccccaatg
     6361 tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc actcctgtga
     6421 tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag aatgccctga
     6481 gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac acttttgtgc
     6541 cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat aacctgcaag
     6601 gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc gcctatccaa
     6661 cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg atgagactgt
     6721 tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca aattacgagc
     6781 agcttaaacc ctatattgat gacaccatgt tgactgacgt gcagcgtgaa accatttttt
     6841 cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca ccgcgctggt
     6901 tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg gtggttgctt
     6961 tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt ggattgccac
     7021 cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg gttgtgcctg
     7081 gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg ggtgaactgt
     7141 ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat agcccacagc
     7201 aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca tccctatggg
     7261 cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt tcgtgctggt
     7321 ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag agcggatgag
     7381 cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt agaagatgga
     7441 cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga acgtctgtct
     7501 tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc tcgcggcgta
     7561 ctactgccac ttctttccct ggactgtgcg gtgacaatca ctaatcggac ggtatcccgc
     7621 gcggaagagt tggctaaatt gtttgcgcac actggcagta ttcaggcgtt gggtatggac
     7681 gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg catcagtggt
     7741 gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta tgacatgttc
     7801 tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc aaagcgtaat
     7861 gctgatggtt taggaatgct ggtggcacag gcggctcatg cctttcttct ctggcacggt
     7921 gttctgcctg acgtagaacc agttataaag caattgcagg aggaattgtc cgcgtgaatc
     7981 aggccatcca gtttccggac agggaagagt gggacgagaa taaaaaatgt gtatgttttc
     8041 ccgctctcgt gaatggtatg caactgacat gcgcgatctc tggcgagagt ctggcgtatc
     8101 gctttactgg agatacgcca gaacagtggt tagcgagttt tcgtcagcat cgctgggacc
     8161 tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa ggctgggtct
     8221 ggttaccctg atccagatat tcgtccttcc atttcacgta attattcgcg gaatagcgta
     8281 acccagcctt ctcttcatca cttaacgggc ggatctgttt gacggggcta ccgagataca
     8341 gatatccgct ctccagccgt ttattttgtg ggaccagact acccgcacca atcatcacat
     8401 catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa actcgattgc
     8461 caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg ccaatggtta
     8521 atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata ctgccatcct
     8581 ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc acaacgagcg
     8641 gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacactg ctatcgtcga
     8701 tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa acatcagaca
     8761 tacttacctc agcaataaat gatttactaa tgactttggg ggcattattg gccttgtgca
     8821 agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa agggtgaaaa
     8881 aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca aaatattggg atccctataa
     8941 tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa caacgaagag
//
LOCUS       DAJTRQ010000143         8937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000143 DAJTRQ010000000
VERSION     DAJTRQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..2886
                     /locus_tag="OO332_003536"
                     /pseudo
     CDS             1..2886
                     /locus_tag="OO332_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO332_003537"
     CDS             complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO332_003537"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5943868.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3978..5330
                     /gene="yegD"
                     /locus_tag="OO332_003538"
     CDS             3978..5330
                     /gene="yegD"
                     /locus_tag="OO332_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5943869.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO332_003539"
                     /pseudo
     CDS             complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO332_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7279..8040)
                     /locus_tag="OO332_003540"
     CDS             complement(7279..8040)
                     /locus_tag="OO332_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5943870.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8037..8696)
                     /locus_tag="OO332_003541"
     CDS             complement(8037..8696)
                     /locus_tag="OO332_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5943871.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2176 a         2369 c         2330 g         2062 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct ctgtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc caattttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggttacgaa
     2101 ccacagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg
     2161 atgattaaag agaatcagtt gatgatgatc gcccacggtg tcgcttcgcc gcggatcccg
     2221 gaagcgcgta atttgtggct gatttcactt aagctctgga gttgcgaagg cgagattatt
     2281 gatgaacaaa aatttcgtcg tagcttcagc gatccggcac ttagccatgc tcttgaccga
     2341 cgggtattcc acgaattttt ccagcaggcc gcaaaagcgg ttgccagtaa aggcataagc
     2401 atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     2461 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     2521 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     2581 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     2641 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     2701 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     2761 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     2821 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     2881 aactgagaga gcgtttgccc attgcctgat gcgatgctaa cgcgtcttat caggcctaca
     2941 aatcgctcat tccgcaggcg ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg
     3001 cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg
     3061 gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccaga aatcgctgtt
     3121 taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat
     3181 aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca
     3241 catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca
     3301 gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcggg tcggctgctg
     3361 ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat
     3421 aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc agttggccta
     3481 aaatcgcccg cacgccctgc tcaaaagcat caacactgcc gggtaaacgc aatccgggcc
     3541 gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa
     3601 gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat
     3661 ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc
     3721 ccactgccag actacgggca taataactgt ccgcgaccgt ttccacacca ctcaccgcac
     3781 gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag ttcaggatat
     3841 acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg ttacgctttg
     3901 ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc
     3961 ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg
     4021 cggtcatgcg tgacggtaaa ccgcatttgc taaaaatgga aaacgacagc acgctgctgc
     4081 cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg
     4141 acgttccggc agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc
     4201 gcgaagaaga tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac
     4261 agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg
     4321 ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt
     4381 tgcacattcg ccagcaggcg caggcgcagc tgccagaagc aattactcag gcggtgattg
     4441 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4501 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg
     4561 tagcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg
     4621 atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag tggcgttcgc
     4681 gtctcgatcg tgaagccagt ctgctgggtc acagtggttg tcgtattggc ggtaacgatc
     4741 tggatatcgc cctagcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg
     4801 aaaaaggcat cgccctgccg atcctgccgt ggtggaatgc ggttgccatc aacgacgttc
     4861 ctgcacagag tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg
     4921 atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct
     4981 atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta gcggaaaccc
     5041 gcgcctcact gccgtttatc agcaatgaac tggctacgct gattagccag cgagggctgg
     5101 aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gccctggata
     5161 acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt tctccgctga
     5221 ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg
     5281 gctccgtcac cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg
     5341 ctcagcagcg gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
     5401 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
     5461 cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggccattttc gccattaccg
     5521 cctgtctgtc tgctggcgtt accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc
     5581 tcgctttcca gtcgatgacc gcctgggtca gatgatcacc aaacccttta acttgcttaa
     5641 cgccacgacg ggtaacacct gctgctgttt caataccaaa agaccataac gccgctttac
     5701 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
     5761 acttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg
     5821 gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt
     5881 ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat
     5941 taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa
     6001 taatccagat agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc
     6061 cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc
     6121 gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt
     6181 gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat
     6241 ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
     6301 ccagcgtaca ccacaggcag tcggtcaaat gagcgggata aacatgcatt gccgaaacgg
     6361 gacatttctt taactgttgg cgcagagaat caagcgccgc tacccacgct ttagccgtcg
     6421 gacgcccggt tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
     6481 tcggtaacat cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat
     6541 ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag
     6601 agataagcgg cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca
     6661 acgcaaggcc aaaattaccg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg
     6721 tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
     6781 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg cgacctacca
     6841 taaaactgtt ctggtttacg tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag
     6901 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt
     6961 gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
     7021 tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga
     7081 cataattcaa caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
     7141 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat
     7201 aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcgccagtag
     7261 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
     7321 cgtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
     7381 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg
     7441 cggagaatta tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac
     7501 tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt
     7561 aatgaagtgc gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc
     7621 gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
     7681 cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga actgctaatt ctttggcttc
     7741 cgcctcggca aaaagtcgct gacgaacggt cagcacaata tccgtggcga ggatatcatt
     7801 aagccccaat tccccaccct gcactttttg cgacatataa gccatcgctt cattgacggc
     7861 gagcatcgcg ccttcgcccc cctgcgaaac gctacctgcg ccatcagcaa ggaacatcac
     7921 cagtaatggc tgctggtcat ttagccaggc aacctgcatc tggcaggcat cctggcaagg
     7981 caagtccgcg ctgatgtgcg acgttccaac agcggatgca taaaccagac gccagctcac
     8041 actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa
     8101 accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa tccttgcaga
     8161 ggcaaaggct gacgaacgct gatttgtgcc agagtcttca tatccgcccc ctgaacaccg
     8221 atggaaaaga aagcaaacct tttatcttct tccccccgaa acactttgtt ggctgcagcc
     8281 tgccactcat cggttggtgc accatcggta atcaggaaaa tccacgggcg ataatacgaa
     8341 ataccatttg cacgatactc acgtttccgc tcctctacca tatccagggc tttggtaatg
     8401 gccgcaccca ttggtgtatc gccctgggca aacaggatgg gcggaaaaaa attagcggcg
     8461 ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caatgccgag ttccactctt
     8521 tttaaagcca gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggtgttaagt
     8581 tcattaatag gtctgccact catcgagcca gaaacatcca gcagcagaat gcaggggcaa
     8641 cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga
     8701 aatcctttct tagagaatta ttgaggtccg gtatcatacc gactgcaata atgaaataag
     8761 agcaaacgaa gaaactttca gaattagtcc cggcggcgca gaaaattttg ctattcgcct
     8821 cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga
     8881 gggttaggga gaggtttccc ccgttacttt attcgtaccc cttataatgg ggtgtta
//
LOCUS       DAJTRQ010000144         8632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000144 DAJTRQ010000000
VERSION     DAJTRQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(8..2551)
                     /gene="mdoH"
                     /locus_tag="OO332_003542"
     CDS             complement(8..2551)
                     /gene="mdoH"
                     /locus_tag="OO332_003542"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5943872.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2544..4079)
                     /gene="mdoG"
                     /locus_tag="OO332_003543"
     CDS             complement(2544..4079)
                     /gene="mdoG"
                     /locus_tag="OO332_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5943873.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4473..5630
                     /gene="mdoC"
                     /locus_tag="OO332_003544"
     CDS             4473..5630
                     /gene="mdoC"
                     /locus_tag="OO332_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5943874.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5638..7119)
                     /gene="clsC"
                     /locus_tag="OO332_003545"
                     /pseudo
     CDS             complement(5638..7119)
                     /gene="clsC"
                     /locus_tag="OO332_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7061..7594)
                     /gene="ymdB"
                     /locus_tag="OO332_003546"
     CDS             complement(7061..7594)
                     /gene="ymdB"
                     /locus_tag="OO332_003546"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5943875.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7689..8000)
                     /locus_tag="OO332_003547"
                     /pseudo
     CDS             complement(7689..8000)
                     /locus_tag="OO332_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8121..8453)
                     /gene="csgC"
                     /locus_tag="OO332_003548"
     CDS             complement(8121..8453)
                     /gene="csgC"
                     /locus_tag="OO332_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5943876.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8512..>8632)
                     /locus_tag="OO332_003549"
     CDS             complement(8512..>8632)
                     /locus_tag="OO332_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5943877.1"
                     /translation="MTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2160 a         2241 c         2045 g         2186 t
ORIGIN      
        1 tacgttttta ttgcgaagcc gcatccggtt tacgcaatgc cagtggattg agctttatcc
       61 cttcgtaata actcacccat gaagaatatc tttcagggga attccagacg cggaaatgca
      121 gacgggccat cgtcaccgga tcgcttagca gcaccaggcg acgatcgcga ttcagcttct
      181 ctggcgtctc gttaagcgcc tgttcaacgt ggcggtcacg ggcgatttcc agcaccttac
      241 tggcgcggtg acgcgctgtc gccattgcgg ttgccagagc gttaaatgac gggttaaaca
      301 ctgcgtgcat aaagccatcg tcaagggagc gttggcgatt catctcaagg aaccgatcgg
      361 tatcaaccag cacctgcggc ggtgaatact cttccgggat caggaacagt ttccagcgtt
      421 tggtgcgcag accaacggtg gcacggctgg aaatcaccga aacaaacggt gacaggatca
      481 acgagaagac aatcggtgcc agccagaaca ggaaacgcag atccagccac gccatcccaa
      541 cagcccacac taaccccaac agcagctgtg agccgtggcg tttgaacgct tcgccccatg
      601 aagtggagtc atcatcacgc tgcggtgaat tccacaccac ttcccagcca aggaacgcgc
      661 tgacaacgaa gaccgtatgg aacagcatgc gtaccggagc cagcagcacg gaaaacagca
      721 cttccagcag caacgataat gtaacgcgcc agaagccgcc gtattctttc gttcctttgc
      781 accagataag caaaatgctc aataacttcg gcaggaacaa cagcaccatg gtcgaagcaa
      841 aaagtgcaat cgccagctca ggacgccact gcggccacac cgggaacaac tgccgtggtt
      901 gcaggaagta ttgcggttcg gtcaacgcat gcactacctg caatgcagta gagagcgcga
      961 ggaacataaa ccacagcgga gcggagagat aagacatcac gcccgtcagg aacaccgcac
     1021 ggtgaaccgg gtgcataccc ttaaccagga acagacggaa gttcatcagg ttaccgtggc
     1081 accagcggcg gtcacgtttt agctcatcaa gcaagttagg cggcaattct tcataagaac
     1141 ccgggagatc gtaagcaatc cagacccccc aacctgcacg gcgcatcaac gccgcttcta
     1201 cgaagtcatg tgacaggatt gaaccggcaa aggaaccttc gcccggcagc ggagccagtg
     1261 cgcagtgctc gataaatggt ttcacgcgga taatcgcgtt atgtccccag tagtgcgact
     1321 cgccaagttg ccagaagtgc aaaccggctg taaacagtgg cccatacacg cgggtcgcga
     1381 actgctgaca gcgcgcatac agcgtatcca taccggacgc tttcggcgac gactgaatga
     1441 tcccggcgtt cgggttggct tccatcaagc gcaccaggcc gcataaacaa tcaccggtca
     1501 ttaccgagtc agcatccagc accaccatgt agctgtactg gctgccccag cgacggcaga
     1561 agtcatcgat attaccgctt ttacgcttca cgcggcgacg gcggcggcga tagaaaatct
     1621 gaccttcgcc accgacttca gcgataagct ccatccaggc tttttgctct gcgacgcaga
     1681 tatccgggtt ataactgtca ctcagaatgt agacatcaaa gtgtttggca ttcccggtgg
     1741 cttttactga ttcccacgtt gcacgcaagc cagcaaaaac acggttcacg tcttcgttac
     1801 agataggcat gatcaacgcc gtgcgatgct ccgggtttaa tggttcatcg ccaactgttg
     1861 acgcagatat actgtattta tcgcgaccaa taagcagttg caggaagccc atcaacgccg
     1921 tccagaatcc ggcggacacc caacagaaca gtaccgcaaa aaggatcagg ataccggttt
     1981 gcagcatata aggcagaagc tgcataaagg aaacccacaa atcctgacca accatatcca
     2041 taggattaat cagcgcccaa ccctgataag gaagaatggt cttcatatac caggtcgcga
     2101 caacagtttg cgcgagcgtc aggatcaaca gaatgtaacg gcggatggta ccaacggtac
     2161 gccacttttg ctcgctctcc tgctcttctt tggtcaaacg agccagatag cgcggcgtga
     2221 catcgcgtcc acgcaggcga tcccagaaac ggcctaccgg gttggtacgc cacgggtcgg
     2281 gaaacatcga ggagcgtttt gcttctggca tcgccttaag ctgatcgcgc ccttcgtcgt
     2341 ctttaattaa ctgtccatca gcaagtgaat ctggccaggc ttgttccaga cgcgccttta
     2401 ccgagccttg cggggaatca tcctcccgcg cccaggtgcg gtgttcggca tccagcgcct
     2461 gatgaacggc gcggatatca gtcttcggca atgctgcttt ctcgcttgcg gcgatgggca
     2521 ttgcgtcaat gtactcagtt gtcttattca ttggcaggta actggtagct ccaggtttca
     2581 ctcaacgtct gatcggcatt caccagcgca gcacgcattt cagtggtttt cttggcatct
     2641 ttcactttca cacgcatcac cagacgccag cctttggtaa ccgggttata gcgcaccgtg
     2701 ctttcaacta tctcaccatt atcaccaatg ctggtttgcg ctgtgaccgg ggtatcctct
     2761 ggcagttttt tcatctctgc gccggtaaaa tcgaccacaa aggcgatagt accgtcaggc
     2821 tggcgaatca ggttcgactg cttcacatcc cccgttgaac gacgcgtttg ttgcacccat
     2881 gcgttatctg gcgcatgcag tttgtcttca tcacggctga aggtgatggt gtatttaaag
     2941 ttcatctctt tacccggctc cggcagctga tccggcgtcc agtaagcgac gatgttatcg
     3001 ttggtttcat cgttggttgg aatttccacc agctcaacgc tgcctttgcc ccactcccct
     3061 ttcggagtca cccatgcgct tggacggaga tcgtaacgat catcgagatc ttcaaagcgg
     3121 gagaaatcac gacctcgctg caacagacca aagcctcgcg ggttttccat ggagaagctg
     3181 ctgaccgcta aatgtttcgg gttattcaac ggacgccaga tccattcgcc attaccagca
     3241 tggatagaca gaccgttaga gtcgtgcaac tccggacgat agttatttgc aggcgacggt
     3301 tggttcggcc caaacaggaa catactggtt aacggtgcaa cccccagttt gccgacttta
     3361 tcgcgcagat agattttcga ctgcacatcc acaaccgtgt cacgccctgg cataaccacg
     3421 aatttgtaag cacctgtcgc acgcggcgag tcaagcaatg cataaatggt taaacgttta
     3481 tcagtcggtt ttggacgctc gatccagaac tctttgaagc gtggaaattc ttcacccgat
     3541 ggcaaagcgg tatcaattgc taggccgcgg gcagaaaggc cataaacctg acctgcacca
     3601 atcacgcgga aatagctggc cccgagcatg ctgacgattt catcgttttt atctttgctg
     3661 ttgatcgggt aaagcacttt aaaaccggca aaaccaaggt cttttaccgt gtctttgtca
     3721 tgctgaacat cgccgaaagt gaaataatcc gggctgtatt tgattcgttt gactgcggtg
     3781 gcagtcactt catttatttt gaccggggta tcgaagtaca taccctgatg gtagaactcg
     3841 agtttgaatg gggtcttcag attgttccag tacgctttgt catgattaaa ctggatctgc
     3901 tgatagtccg cgtatttcat gtcgcggaaa acggagggca agttgctttt aggcgcctca
     3961 tagcctttcc cggctaagga ttgagcttgc tttgcgacat catcaatact gaaagcccag
     4021 cttgaagatg tatacagggt taacattact gcagcactca accaacgcat tttcatcatt
     4081 tgtagtttat gtttcataat tagtaagcac ttcccccttt gtgtgcttat atcgatccga
     4141 tctattttaa tggataacca gccattccga caactgaatc ccggtatcgt tcagcacgct
     4201 ggctcatgga ttaagcacct gattgcaacc gttttcactt tccgtgcttc tcctaaagac
     4261 agaagcatcg acttcgtgta gggtttgcca gcaaatttat ttatcaaccg tcttacagac
     4321 gcttccattc tggttagtgg tagcgatgat gctatgcata aaaaccttga agcctgcgcc
     4381 tggcgcggtc aggtcggtgt cgctatggca acaagcctaa aggcataacc cgacagggat
     4441 aagacgaaaa atccgagatt agttaaccat atatgaatcc agtacccgcg caacgtgaat
     4501 atttcctcga ctccatccgc gcctggctga tgttgttagg gatccctttt catatttctt
     4561 taatctattc gagccataca tggcatgtga atagcgccga accgtcattg tggctgaccc
     4621 tttttaatga cttcatccac tcgttccgca tgcaggtatt tttcgttata tccggatact
     4681 tttcctacat gcttttttta cgctatccct tgaaaaaatg gtggaaagta cgtgtcgaac
     4741 gtgtaggtat cccgatgtta acagccatcc ccctactgac attaccgcaa tttattatgc
     4801 tgcaatatgt caaagggaaa gcggaaagtt ggcctgggct atcattgtat gacaaatata
     4861 atacgttggc ctgggaatta atatcacacc tgtggttttt actggtgtta gtagtcatga
     4921 cgacgctgtg tgtatggata tttaagcgca tcagaaataa tttagaaaat tctgataaaa
     4981 cgaataaaaa attctcgatg gtaaaactat cggtgatttt tttatgcctc ggcatcggct
     5041 atgcggtaat aagaagaacg atttttattg tgtatccacc cattctgagt aatggcatgt
     5101 tcaattttat tgtcatgcaa acgctatttt atttaccgtt ctttatcctc ggcgcactgg
     5161 ctttcatttt ccctcatctt aaagccttgt ttaccacgcc gtctcgtggc tgtaccctcg
     5221 ctgcagcatt ggcgtttgtc gcttatttac tcaaccagcg ctatggcagt ggcgatgcct
     5281 ggatgtacga aaccgagtcg gtgatcacca tggtcctcgg cctgtggatg gtgaatgtgg
     5341 tcttctcctt tggccaccgt ttacttaact tccagtcagc gcgggtgact tattttgtta
     5401 acgcatcgct gtttatctat ctggttcacc acccgttaac gctgtttttc ggcgcataca
     5461 ttacaccgca catcacctcc aactggcttg gttttctctg tggcctgata ttcgtagtag
     5521 ggattgcgat aattctgtat gaaattcatt tacgcatccc gttactgaag tttttgttct
     5581 ctggtaaacc ggttgttaag cgtgagaacg ataaagcacc agcccgttaa gccacattta
     5641 caataaccat tccacgggca atatcgacgc cagtctgacc ataacccgct tccagaaact
     5701 ggtggcgggt tcttttttga gaataacctc tttctttgca tgacgatcaa cccagttgat
     5761 ccgtccccac ctgtccagac ggagctgcca ggccgcatcg tactggctct gaataaagcg
     5821 tttatcaatt aactgtgcca gcgtttcgct ctctatcacg aagcccattt cagtattgag
     5881 caatgttgaa cgcggatcga aattgaaaga gccgataaac accgttttac catcgatgct
     5941 aaaagtttta gcatgcaggc tggcaccgga attaccggtt atgccgcgat cgtgtaacgt
     6001 actgctttgt tcacgcgtcg gcttgagttc atataattcc acgccatagc ggagcaattt
     6061 tttgcgccag cgcgcgtatc cggcatggac gacagcaaca tcgttagcgg caagagaatt
     6121 ggttaggatc gcaatcttta ccccttttct caccatccgt aagagttgcg ccacacctgc
     6181 gcgtgtcggt acaaaatagg aagagataat atcgatgcgt tcactgggtg agcccatgat
     6241 atcgaacagg cgctgcggta acagtgaatg ccgttttgcc ttgccctccc ctttcgccgg
     6301 atcatcactt aataaacgtg tcttcgccca gataagcggc aacgttccat caaccagatg
     6361 atttataaat ggactggatt ccattttgcg taaataacga tgcgtcatgg catcgttatg
     6421 ccaggaggcg ggtaactcga tgcgatccgc catttcacct tccgggacat ccagcacctg
     6481 ctgtaagggt gaaaccgatt tgcaatacca gtagcgggcg aaatcatcgg caacgtcctc
     6541 taccacgggg cctattgcca tgacatctaa atccgaaaaa agtcgctctt cccctgcact
     6601 aaaataggca tcaccaatat ttagtcctcc cactagggta accacgccat cgacagtgaa
     6661 acttttattg tgcatccggc gattgagacg ggaaaagtcg gtgatatatc caagcggacg
     6721 tagcaagcga aacgagaaag gattaaaaag ccggacttca atgcgtggat gactgtcaag
     6781 caagcgtaaa atgtcgtcaa gtccgggcgt attgttgtcg tccagcaaca aacggacgcg
     6841 aacgccacgc tttgctgcgg ctaacagggc ggaaaacagt aaccgccccg acatatcgtc
     6901 ctaccagatg taatactgaa catccagcgt atgctctgcc atttcggcca gacgataccg
     6961 ggcggcaaac gcatccagac ttttctccag agggaaaaga ccacactgac cgggatgttg
     7021 cgaacacagt ggcagcaccg cgctcgccag ccggggcaaa tcattcatct ccttgttggg
     7081 taaggagtct ttcgtagagg tgggcgtttt cttcatcata acagacaaag tatacctgtt
     7141 cgggtaaagc gtgacgggta ataaattctg aaacggtttt aactgcgatt tcagccgctg
     7201 ccgcacgagg gtaaccataa accccagtac tgattgcagg aaaagccact gacgtatagc
     7261 tgtttgccgc caccagtcgt aggctattca gataggcatc ctgcaaaagc tggtcttcgt
     7321 tttgttcacc accacgccag actggcccga cggtgtgtac tacggcttta gcgggaagat
     7381 cgcctgcaag cgtaataacg gcatgccccg tagggcaatc gccctgctgt tgcctgactt
     7441 ttaaacaggc atccagcagg gccggacccg ctgcgcgatg aatggcccca tcgacgccgc
     7501 cgcctcccat taatgacgga ttagccgcat tcacaatcac atcaacggcc agtttggtaa
     7561 tatcaccctg cacaacatga atacgcgttt tcataacgcc tccttacacc ttcttgtttc
     7621 ttaagtgtac agcaggcttt ttggttaaat cgcatttttc tgctcagcaa cgtgagttgt
     7681 tcagaaactt agttatattc gatgttcatt acagccagct ttttctgttt atcaagatac
     7741 tgcactttta ctgaggctat ttgcgcgttt ttgacattgc cgctcattaa cgctttgctg
     7801 gagtaggttt tgcccggaat agaaccattc tatgggcagt tcatttctac gggtgatgac
     7861 tgggtgctga cgtcacatgc cggttccaca atttggccat aaaaatggat cacgccccct
     7921 tgcgcggtgc ttcctgcaat ggcaataaaa ggaaaaaaca aactgccgag cataagagag
     7981 tctaaaaaag gtctgaacat ttattctatt tcctcaatga ttagtcatcc ttgagggttg
     8041 tgttatccat actttcaggt tggctgattc gcagcagacc attctctcca gattcatctt
     8101 atgctcgata tttcaacaaa ttaagacttt tctgaagagg gcgtccattg ttgtgataaa
     8161 tgaagtgcct gtccatcaga aacagtaaca actattttca cccgatcttc cggggaaata
     8221 tttaaactca acttcgtcaa agcaatgggt tgattagcag gcaatgagag ggtcttttct
     8281 tgcttcgtct gactttgccc tgaactgcct tcgcgcaggg acaatatttg tactctgcac
     8341 agacaagatt gagtaagagt aacttcagga ataatggtgt acatatcccc ttgctgggtc
     8401 gtattaaagg ttatctggct ggaaagtgcc gcaaggagta ataacgcatt catattcttc
     8461 tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata caaatgatgt attaatactg
     8521 atgagcggtc gcgttgttac caaagccaac ctgagtcacg ttgacggagg agttcgatgc
     8581 agtctggtca actgcagcac cgttgccacc accgaactgt ttaacagtca tt
//
LOCUS       DAJTRQ010000145         8626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000145 DAJTRQ010000000
VERSION     DAJTRQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..390
                     /locus_tag="OO332_003550"
     CDS             <1..390
                     /locus_tag="OO332_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943878.1"
                     /translation="LAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFIS
                     MALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSF
                     NLALVIAACVTFTGALCYFFIVKDRIE"
     gene            642..1559
                     /gene="oxyR"
                     /locus_tag="OO332_003551"
     CDS             642..1559
                     /gene="oxyR"
                     /locus_tag="OO332_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5943879.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1542..2942)
                     /gene="sthA"
                     /locus_tag="OO332_003552"
     CDS             complement(1542..2942)
                     /gene="sthA"
                     /locus_tag="OO332_003552"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5943880.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3263..3913
                     /gene="fabR"
                     /locus_tag="OO332_003553"
     CDS             3263..3913
                     /gene="fabR"
                     /locus_tag="OO332_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5943881.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3913..4272
                     /locus_tag="OO332_003554"
     CDS             3913..4272
                     /locus_tag="OO332_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5943882.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4312..5412)
                     /gene="trmA"
                     /locus_tag="OO332_003555"
     CDS             complement(4312..5412)
                     /gene="trmA"
                     /locus_tag="OO332_003555"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5943883.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5526..5716
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5781..7625
                     /gene="btuB"
                     /locus_tag="OO332_003556"
     CDS             5781..7625
                     /gene="btuB"
                     /locus_tag="OO332_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5943884.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7570..8427
                     /gene="murI"
                     /locus_tag="OO332_003557"
     CDS             7570..8427
                     /gene="murI"
                     /locus_tag="OO332_003557"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5943885.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1996 a         2095 c         2298 g         2237 t
ORIGIN      
        1 ctggcgaaaa aaggctacga cctggcgaag acgcgtaaaa cggctattgt ctgcggtttg
       61 atgatgtcgg cattaggcac gttgctggtc gtgcaatctt cctcgccagc tcaggcggta
      121 gcgtttatct caatggcgct gttctgcgtg catttcgcgg gaacgtctgc atggggactg
      181 gtgcaggtga tggtgtcaga aacaaaagtg gcttccatcg ccgggattca aaactttggc
      241 agttttgttt ttgcttcctt tgctccgatc gtaaccggtt gggtagtgga taccacacac
      301 tcgtttaatc tggcgctggt tatcgcggcc tgtgtgacgt tcaccggggc gctgtgttac
      361 ttctttattg tcaaagatcg cattgagtaa tcgttactat caggctctct tgctgtgggc
      421 ctgtagaata aaaaaaagcg gatcctggag atccgcaaaa gttcacgttg gctttagtta
      481 ttcgagttga gaaactctcg aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc
      541 cgtttctgtg agcaattatc agtcagaatg cttgataggg ataatcgttc attgctattc
      601 tacctatcgc catgaactat cgtggcgatg gaggatggat aatgaatatt cgtgatcttg
      661 agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat tcctgccacg
      721 ttagccagcc gacgcttagc ggccaaattc gtaagctgga agatgagctg ggcgtgatgt
      781 tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg ctggtggatc
      841 aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc cagcagggtg
      901 agacgatgtc cggaccgctg catattggtt tgattcctac ggttggacca tacctgctac
      961 cgcatattat cccgatgctg caccagacat ttccaaagct ggaaatgtat ctgcatgaag
     1021 cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc gtgatcctcg
     1081 cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag ccaatgttgc
     1141 tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtactgatg gccgatctgg
     1201 caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt
     1261 tctgctttga agccggggcg gatgaagata cacacttccg cgcgaccagc ctggaaactc
     1321 tgcgcaacat ggtggcggca ggtagcggga tcactttatt gccagcgctg gctgtgccgc
     1381 cggagcgcaa acgcgatggg gttgtttatc tgccatgcat taagccggaa ccacgccgca
     1441 ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag cagctggcag
     1501 aggccatccg cgcaagaatg gatggccatt tcgataaagt gttaaaacag gcggtttaaa
     1561 ccgtttaacg cagctacccg ataggcttcc gccatcgtcg ggtagttaaa ggtggtgttg
     1621 acgaagtact caatagtgtt gccgccacct ttctgttcca taatcgcctg accgatatga
     1681 ataatttcgg cagcgcgctc gccaaagcag tgaataccca gaatctcttt tgtttcccga
     1741 tggaacaaaa ttttcagcgt gcccacgttc atgccgacga tttgtgcacg tgccagatgt
     1801 ttgaactggg cgcggcccac ttcatacggc actttcatcg cggtcagctg ctgttcggtt
     1861 ttgcccacag agctgatttc cgggatggtg taaatgccgg tagggatatc ttcaatcaga
     1921 tgtgcggtgg cttcgccttt taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc
     1981 gctgatgcca ggctcggata accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg
     2041 gtctgataca tgctgttgac cttcagctgt ccgcgactgt cagtttccag cccaatgttc
     2101 tgtaacgcca gcgaatcggt attaccggta cgaccgttgg catagagcag gcagtcagct
     2161 ttcagttttt tacccgactt cagatgcatg atcacaccgt cgtcacagct ttcgatcttc
     2221 tcgtactctt cgttgtgacg aatcactacg ccactgttcc agaagtgata ggagagagaa
     2281 tctgacatct cttgatcgag aaatgccagc aggcgatcgc gggtgttgat cagatccact
     2341 tttacatcca taccgcggaa gatcgacgca tattcacagc cgatcactcc agcaccatag
     2401 ataagtacat ggcgcggttc gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt
     2461 ggatgggtga aatcaacatc tgttggatga tatggacgag agccgcaggc aataacaaat
     2521 ttttcagcgg ttagtgtttc aacgctgccg tccgggcaat ccagcgccaa cgtatgctcg
     2581 tcaacaaagc gagcgttccc ctgcaatatt ttacagtgat tacgttcgta aaatccctga
     2641 cgcatgcgcg tttgttgatt aatcacgtta tcggcatggt taaggatatc ggcaaaagaa
     2701 gagcggagca gtcgggaatg gtcgctgtat agtgggtttt gattgaattc tataatgcgg
     2761 ctgacggcgt gacggagagc tttcgacggg atggtgcccc agtgggtgca accgccgcca
     2821 acattttgat aacgctcgat aactgcgacg cgcgcacctt gcttaaccag gcccattgca
     2881 gcgccttcgc cgccggggcc ggaacctatt actatggcat cgtaatcgta ggaatgtggc
     2941 atggtagggc ttacctgttc ttatacataa aatcaacata atggtaacat tctatcgcgg
     3001 gtaagccaat tgatccccgt catttatctg gctatatcct gagcggcctt tgctttgtct
     3061 gtttcttact tttgccctga cgttttattg gatttttatc gacgatactc tccgtttaag
     3121 cggcaggttt ccgctgtacg taaaagaacc ggccagtaaa tatgttttat tgcgttaccg
     3181 ttcattcaca atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac
     3241 ttttggcaag gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg
     3301 ccgttcgctg gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct
     3361 gagtttgcgt gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt
     3421 ccgcgacgta gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca
     3481 actcatgcgc caggcacgtc agcgtatcgc caaaggcggg agtgtgatcc gtacctcggt
     3541 ctccacattt atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga
     3601 acgctccggc acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat
     3661 tgcggaactt gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc
     3721 gcaagccgaa gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg
     3781 cgtcgaacaa cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa
     3841 aggggcttat tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt
     3901 gaaggacgag taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttggtt
     3961 gctggcttat cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct
     4021 gtattcccga ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat
     4081 cgcaccatgt cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg
     4141 ctgtacagca cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca
     4201 gtactctccg tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa
     4261 gttgctgagt aatcggtatc atgccggatg ctggcgcatc cggcatgggt tttacttcgc
     4321 ggtcagtaat acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag
     4381 acgttcgacc ttgtgcgtct ggcttaatgt ttccagattt ttgcataacg tttccggatt
     4441 gcaggagata tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag
     4501 accgctgcgc ggtgggtcga caaaaatggt ttcgaactga tagctcttta agtcgatccc
     4561 ttgcaggcgg ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat
     4621 acgaataatt tgtacgttat caatatggtt agcggcaatg ttgtattgcg cggcagcgac
     4681 tgacggtttg gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gcgctaatga
     4741 aaagttaccg ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc
     4801 cagcgcccat tccagcattt gaatattcat cgccgcgttc ggctgggtaa agctgttttc
     4861 tacctgacga tagatcatct ctttccctgc gaccggcaga cgctcgtcga tgtaatcttg
     4921 atccagcgcg attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc
     4981 gcgcagtgca tcgcgcagtg cttccgcttc ctgacgccac tcatcatcca gctttttatg
     5041 gtacagcagg gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa
     5101 cttgtggcgc agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt
     5161 gatgagttca ctggcggcgg ggaagctatc cacgcggatg cggcttttgg tttgttgatc
     5221 gaaaatgatg tgatacaggt catcgccatc gtgccagatg cggaactccg cgcgcatccg
     5281 gtaatgactg accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat
     5341 actttgcaaa cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg
     5401 ttcgggggtc atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca
     5461 tcagatgtcc agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt
     5521 acaacctgta gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatcta
     5581 gagctgacgc gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc
     5641 ggtgggaagt catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg
     5701 ccaacgtcgc atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct
     5761 tctattgtgg atgctttaca atgattaaaa aagcttcgtt gctgacggcg tgttccgtca
     5821 cggcattttc cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctatcc
     5881 gttttgaaca gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata
     5941 tcgaccgctg gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata
     6001 tcacccaaaa cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca
     6061 gtcatgtgct ggtgttaatt gatggcgtcc gcctgaatct ggcgggggtg agtggttctg
     6121 ccgaccttag ccagttccct attgcgcttg tccagcgtat tgaatatatc cgtggaccgc
     6181 gctccgctgt ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg
     6241 atgaacccgg aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg
     6301 tctctacgca gcaacaactg ggggataaaa cacgggtaac gctgttgggc gattatgccc
     6361 atactcatgg ttatgatgtt gttgcctatg gtaataccgg aacgcaagcg cagacagata
     6421 acgatggttt tttaagtaaa acgctttatg gggcgctgga tcataactgt actgatgcct
     6481 ggagcggctt tgttcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt
     6541 ctcccggttc accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc
     6601 tgcgctataa cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag
     6661 attacaacta cgatccccat tatggtcgtt atgattcgtc ggcgacgctc gatgagatga
     6721 agcaatacac cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg
     6781 gtgtcgactg gcagaaacag actacgacgc cgggtacagg ttatgttgag gatggatatg
     6841 atcaacgtaa taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg
     6901 aaggcgcagc acgcagcgac gataactcac agtttggtcg tcatggaacc tggcaaacca
     6961 gcgccggttg ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata
     7021 aggcaccaaa tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga
     7081 aaagcaaaca gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt
     7141 ccggatatcg taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt
     7201 acaacgaagg gaaagcgcgg attaagggcg tcgaggcgac agccaatttt gataccggac
     7261 cactgacgca tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc
     7321 cgttgttacg ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact
     7381 tcgactgggg tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt
     7441 atccttatca aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc
     7501 cggtcacctc tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg
     7561 agacagtcta tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct
     7621 tctgaaccac gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat
     7681 gacgagatcc ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct
     7741 ttcccgtatg gcgaaaaaag cgaagcgttt attgttgagc gagtggtggc aattgtcacc
     7801 gcggtgcaag aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc
     7861 gtttcacttc ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg
     7921 attaaacctg ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga
     7981 acagttaaac gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata
     8041 gaaatgctgg gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat
     8101 gtttctctgg atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca
     8161 gataccgttg tattgggttg cacccatttc cctctactac aagaagaact gttacaagtg
     8221 ctgccagagg gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcttgg
     8281 ttgttagaac atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg
     8341 gcaatgacgc cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg
     8401 ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt tgcgcggtcg gaaaattatt
     8461 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
     8521 caacggcaaa cacgccgccg ggtcagcggg gttctcctga gaattccggc agagaaagca
     8581 aaaataaatg cttgactctg tagcgggaaa gcgtattatg cacacc
//
LOCUS       DAJTRQ010000146         8589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000146 DAJTRQ010000000
VERSION     DAJTRQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..264)
                     /locus_tag="OO332_003558"
     CDS             complement(<1..264)
                     /locus_tag="OO332_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5943886.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGR"
     gene            complement(274..822)
                     /locus_tag="OO332_003559"
     CDS             complement(274..822)
                     /locus_tag="OO332_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5943887.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(822..1529)
                     /locus_tag="OO332_003560"
     CDS             complement(822..1529)
                     /locus_tag="OO332_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5943888.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1544..2221)
                     /gene="ydjY"
                     /locus_tag="OO332_003561"
     CDS             complement(1544..2221)
                     /gene="ydjY"
                     /locus_tag="OO332_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5943889.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2226..2936)
                     /locus_tag="OO332_003562"
     CDS             complement(2226..2936)
                     /locus_tag="OO332_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5943890.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3103..3909)
                     /gene="xthA"
                     /locus_tag="OO332_003563"
     CDS             complement(3103..3909)
                     /gene="xthA"
                     /locus_tag="OO332_003563"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5943891.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4355..4933
                     /locus_tag="OO332_003564"
     CDS             4355..4933
                     /locus_tag="OO332_003564"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5943892.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4949..5575
                     /locus_tag="OO332_003565"
     CDS             4949..5575
                     /locus_tag="OO332_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5943893.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5572..7305
                     /gene="astA"
                     /locus_tag="OO332_003566"
     CDS             5572..7305
                     /gene="astA"
                     /locus_tag="OO332_003566"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5943894.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7302..>8589
                     /gene="astB"
                     /locus_tag="OO332_003567"
     CDS             7302..>8589
                     /gene="astB"
                     /locus_tag="OO332_003567"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5943895.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2010 a         2203 c         2354 g         2022 t
ORIGIN      
        1 acgaccggca gcagcttcgg tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg
       61 aacaatcttc aggtttatag cgtaatgggt tttcatctcg ccgctaaccc agtcgagata
      121 gcggttaatt gcggtatcgc cgccccaggc gttaaaccag acggtttgcc ctttggcctc
      181 atttttaatt tcctgccagt cactggcatg tgttgccaaa gaaaacagcg ataacaatcc
      241 cagcaaccac ccacaatggc gcatcttccc tccttaagaa cgtttttccg gaatacggca
      301 cagcccctga gcgggaatgt ccatggcaga attaaaacga gccgacaggt tttgcaggcc
      361 gatcaatgcc gtcagttcgg tgagcgcctg agcgtcaaaa tgcgcagcca gtcgggtacg
      421 cagggcatca tcgactgttg gcggtgtgac gcttgcggct tcggtgtact ccagcgccag
      481 ccgttcttca tcgctaaaga gcgggctttg ccgccagtca gccactgcca gcagtttatc
      541 ggtgctgccg gtacgctcgg cgactttcat actggtgata tccacgcaaa actcacacag
      601 gcacatttgc gcaatgcgcg cgctgacaag cgatcgcact accggatcga gcggtgagcg
      661 tttgcgctcc agccacccaa caaacattga caccaaataa aagataaacg ggatccgccc
      721 ccaccagcga atcgggctta gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg
      781 acgtaaaatg aaaggagttt tgctaagcgg cggtaaaccc atcagtcatt cctcttctgg
      841 cgctcaagcc atattttctt cgccataaaa atcaccacgg tcagggcaaa cagaataaac
      901 agttcggtga caaaccagaa agtaccgcct gttaacatgc tgcccgccca ggaataaaca
      961 atagtcgccg gtaattgacc aagcccggtg gcgataaaaa acgagcgaaa acgtattgaa
     1021 gtcagaccgg cagcatagct gattggatcg aaagggacaa aaggcaataa ccgacagacc
     1081 aggatggtgt gtttgccgta gcgagtgaaa aagccgtcca tactgtcaag cacggttttg
     1141 ccggttaatt tttccaccac ttcgcggccc atcactctgg cgataaaaaa gcacagcgcc
     1201 gcgccggcca tcgaactggt ccacgacagc aagccgcccc agaacgcgcc aaacagcgac
     1261 gcattggcaa aggtgatcaa aaacgcaggt agcggtgcag caatggcctg taaaatcatc
     1321 aacaagaacg agacaactgc cgccagtgcg ccgtaagact gaataaagcg ttctataccc
     1381 tgttggtcca cggcggcaaa cgccgcaacg ctgcgattga taaactcatg gacgccggga
     1441 agcagcgccc atgcgagcag catagcgaac aacaggatga tgagggttat acggtagtac
     1501 cagattttac gcgattgtat cttcatcatc ctttatcctg gctttattcg gtgattttaa
     1561 aggtaaccgt tgccaacgtg ttatccgccg ggagaactga ggcattgcct ttgaatttca
     1621 cttcgccgcg tttttccacc gcaccataag tgtatgttgc attgctgacg atgccgaccg
     1681 ggcagctatc cagacacacc aggcaacccg ttttcttctc ttctgctgcc gttaaattac
     1741 cgccaaagcg catgtccagt ttcttgccat tactgtcaac aatcacttca tcgaaggaat
     1801 acgcttttgc cgccccttgc cagttgaccg aaatatccag tttgctgcct gtgacatgag
     1861 tcgtttcttt attatccatc gtcatgtttt cgcccggcgt accacctgcc tctttcaggg
     1921 cttcataaaa tgctttcggg gtcgcataag ccataaacag cgatttatgt ccgttggagc
     1981 catctttaaa cacaataccg tgacgagtgt cgtcggtgag gaaacgacca ttaatttgca
     2041 ccagcatggt taccgtatgg ttattggcat cgacgcgcag cggttggctg ttcgaaagtc
     2101 cgtcgtattc cacttttgcc gccgcattct ctttttgatc gcagccgcta agcccggcaa
     2161 cagccagtaa gcagagtgca gccagtcctt ttttccatga cactgaataa tgttgcgaca
     2221 tcgttctatc cttcattttt tgggtgagta agtgggctgc ggcgcgaagc agacagatcc
     2281 acatgtttgt gtcgggcgta gagttttgcg agctggatga gaataaacag cgccaggccc
     2341 gccagacaga gttgtaaaat aaagcgcagc gtaatgcctt cattggcgag atcgcttgcc
     2401 atcacggtat aaataacaat accgggcagg gtcgtgagtg ccgaaataag ggtataaggc
     2461 cagaaggcga tggtggttaa tccgtaagcg taattttgaa tattgtaagg aaacaacggg
     2521 attaagcggg tcagaataag aaaatcaata ccgttacgcg caatgccttt ttcaatggcc
     2581 tggaaggtat ggctatggcc cacgtatttc agcagtaaat cacgtcccag ccagcgcgcc
     2641 agcaggaatg agcacgagga ggccagcgtt gcggcaatta atgagagtag tgtccctaag
     2701 agcgggccaa aaactattcc tccagcgatc accagtatgc ttcctggtaa cagaaagagg
     2761 ggggcattga tgaataacag aatatagagg ttataaccga acaatccgct ctggcgaatg
     2821 agtgtctgta agtggggtaa atcggtgagc agatcgaata aaccgaaagc gtggatagcg
     2881 taaatgacca gcgcaaaaat aagacaggca aacagaaagt tacgctcagc gttcatcgtg
     2941 gagtttccta tatcaaactt gataatagag aagtggtcag acaactaaaa acgcagtgac
     3001 ggtataacaa aggacggcag gcaacaaatc attactgaca gtttgttgat ttttctcaag
     3061 gttaattctc ctgacccagt ttgagccagg agagctgcta aattagcggc ggaaggtcgc
     3121 ccagacgggg gcgtgatcgg acggtttttc catgctgcga atttcatagt cgatgccggt
     3181 ttctacgcaa cattctgcca gcggttggct ggcgagcagc aggtcgatgc gcagaccacg
     3241 gttatcgtca aaaccttttg agcggtaatc aaaccatgag aaacgatctg ctgtttgcgg
     3301 attagcatgg cggaaggtat cgaccacccc ccagctcatc agcctgtcca tccattcgcg
     3361 ctcttccggc aggaaagagc atttaccggt acgcagccag cgcttacggt tttcttcgcc
     3421 aatgccgata tccagatctg tagggctgat attcatatcg cccataatca gtaccggatt
     3481 atcacgtttg agttcggttt ccaggtagtt ttgcagattc tgataaaact gcgcttttgc
     3541 cgggaatttt atcggatggt cgcggctttc accctgcggg aagtaaccgt tgatcacggt
     3601 gacataaccc agcagtgagg ggatttccgc cataataatc cgccgctgcg cctcttcgtc
     3661 gtcaccggga aagccgcgac gcacggcaat cggcgtctct ttggtcagca gcgccacgcc
     3721 ataatggcct ttctgcccgt gataaaacac gttgtagccg agcttcgcca cctcttcgag
     3781 cggaaacata tcgtcatgaa cttttgtctc ctgcaggcca atcacatccg gttggtgctt
     3841 ttcgacgatg gcttcaagct ggtgaggtct ggcgcgcagg ccgttgatat taaaagagac
     3901 aaatttcata gtcgctgcca tttattcaga taaagagtgc gtggatggta gcagaatttc
     3961 gcgttgctta ccgcctgttg ttaagtgatt tgatcgatgg ttgttaatga tgtcaacgat
     4021 ggcgcaaaaa atgcccgctt ttggtgcgcg ctgcgtcaga atggcgcagt aatttccagt
     4081 aaatttcgtc aatgatcaca attccagaat tatattttac ttctgcataa cattgcgttt
     4141 tttattcttt ttattcaccc acatcgcaaa cgtattcact ttatatgcac tttaaatgca
     4201 tatggtttgg ttataactcc ttgatttcca gttagcctcc gccgtttatg cacttttatc
     4261 actggctgac acgaaccctg caatctacat ttacagcgca aacattactt attgttaaca
     4321 tacaaataac gaattattta ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa
     4381 ctttgatgaa tggatgatac ctgtttacgc tccggcaccc tttataccgg tacgtggcga
     4441 aggttcgcgc ttgtgggatc agcaggggaa agagtatatc gacttcgcgg gtagcattgc
     4501 ggtgaacgcg ctgggccatg cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag
     4561 taagttctgg cataccggca acggttacac caacgagccg gtactgcgac tggcgaaaaa
     4621 attgatcgac gccacgtttg ccgatcgcgt cttcttttgt aactccggtg cggaagccaa
     4681 cgaagcggcg ctaaaactgg cgcgtaaatt cgctcacgac cgctacggca gccataagag
     4741 tggcatcgtg gcgttcaaaa atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg
     4801 tgggcagcca gcctattcac aggattttgc gccactgccg gcggatattc gtcatgctgc
     4861 atataacgat attaactctg ccagcgcgct gattgacgac gccacctgtg cggtgattgt
     4921 cgagcccatc tagggcgaag gcggtgtggt gccagccagc aacgcgtttt tacaaggtct
     4981 gcgtgaattg tgtgaccgcc acaatgcctt gctgattttt gatgaagtac aaaccggcgt
     5041 cgggcgcact ggtgagctgt atgcctatat gcactacggc gtgacgcccg atctgttaac
     5101 caccgccaaa gcgctgggcg gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg
     5161 cgcccgcgtg atgaccgttg gcactcatgg caccacctat ggcggtaatc cgctggcctc
     5221 ggcggtagca ggcaaagtgc tggagctcat caacacacca gagatgctta atggcgttaa
     5281 acagcgtcac gactggtttg ttgagcgtct taatactatt aatcaccgct atggtttgtt
     5341 cagtgaagtt cgcggcttag gtttgttgat tggctgtgta ctgaatgccg attacgccgg
     5401 gcaagcgaaa cagatctctc tggaagcggc gaaagcaggc gtgatggtac tgattgcggg
     5461 tggcaacgtg gtgcgttttg cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg
     5521 actggatcgc tttgcagctg cttgcgaaca ctttgttagc cgaggttcat catgatggtc
     5581 atccgtcccg ttgagcgctc agatgtctcg gcgctgatgc agcttgccag caaaacgggc
     5641 ggcggcctga cgtcgcttcc cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca
     5701 atcaaaacct ggcaaggcga actgcctaaa agcgagcagg gctatgtgtt cgtgctggaa
     5761 gatagcgaga caggcaccgt ggcggggatt tgcgctattg aggtggcggt tgggctgaac
     5821 gatccctggt acaactatcg cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc
     5881 tataacgcat tgccgacgct gtttcttagt aacgatcaca ccggcagcag cgagctgtgc
     5941 acgctgtttc tcgacccgga ctggcgcaaa gagggcaacg gctatttgct gtcgaaatcg
     6001 cgctttatgt ttatggcggc ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg
     6061 cgcggggtga ttgacgaaca cggctattca ccgttctggc aaagcctcgg taaacgcttc
     6121 ttttcgatgg attttagccg cgccgatttt ctctgcggca ccgggcaaaa ggcatttatt
     6181 gcagaactga tgccgaaaca tccgatctat acccactttt tatcccagga agcccaggac
     6241 gtcatcggtc aggtacatcc gcaaaccgcg cctgcccgcg cggtgctgga gaaagaaggt
     6301 tttcgttacc gcaactatat cgacatcttc gacggcgggc caacgcttga gtgtgacatc
     6361 gaccgcgtgc gcgccatccg taaaagtcgg ctggtggaag tggcagcagg ctggcttgcc
     6421 gccgggcgtg aaacgggtca ggcgctgagt gcgctggagg atctcgacgg tttgctgttt
     6481 accggtagcg ccaataccgg ctaccagctg catcgccagc tctccggtca gccggagaaa
     6541 attctcgccc ttgagatggg cggtaataat ccgctaatta tcgatgaggt ggcggatatc
     6601 gacgcggctg tccatctgac cattcagtcg gcgtttgtca cagccgggca acgctgcacc
     6661 tgcgcccgcc gtttattgct caaaagcgga gcgcagggcg atgcgtttct tgctcgtctg
     6721 gttgccgtca gccagcgatt aacgccgggc aactgggatg acgaaccgca gccgtttatt
     6781 ggcgggctga tttctgaaca ggccgcacag caggtggtta ctgcctggca gcaactggaa
     6841 gcgatgggcg gacgaaccct gcttgcgccg cgcttattac aagcagggac atcgttgctg
     6901 acaccaggga tcattgaaat gacgggcgtt ggcggtgtgc cggatgaaga ggtgttcggg
     6961 ccgcttttgc gcgtctggcg ttatgatact tttgatgagg cgattcggat ggcgaacaac
     7021 actcgcttcg gactcttttg cggtctggtt tcccccgagc gggaaaaatt cgatcaactg
     7081 ttgctggagg cgcgggcggg gattgttaac tggaacaaac cgcttactgg tgctgccagt
     7141 accgcgccat tcggcggcat tggtgcttcc ggtaaccatc gccccagcgc ctggtatgcc
     7201 gcagattact gcgcatggcc tatggcgagc ctggagtcgg actcgttaac attgcccgcc
     7261 acgcttaacc ccgggctgga tttttccgat gaggtggtgc gatgaacgcc tgggaagtta
     7321 atttcgacgg gctggtaggg ctgacgcatc attacgcggg cctgtcgttt ggtaatgaag
     7381 cctctacccg tcaccgtttt caggtgtcta acccgcgact ggcggcaaaa cagggcttac
     7441 tgaaaatgaa agcccttgcc gatgcgggat tcccccaggc cgtgatcccg ccgcacgagc
     7501 gtccgtttat tccggtgctg cgtcagttgg gattcagtgg tagcgatgag caggtactgg
     7561 aaaaagttgc acgccaggca ccgcactggc tttccagcgt cagttccgct tcgccaatgt
     7621 gggtagccaa tgcggcaacg atcgcgccat ctgccgatac gctggatggc aaagtgcatc
     7681 tcaccgttgc caacctgaac aataaatttc accgttcgct ggaagcgccc gtcactgaat
     7741 cgctgttaaa agcgattttt aaggacgaag agaaatttag cgtccattcg gcgttgccgc
     7801 aggtagcgtt gctcggtgat gagggggcgg caaaccacaa tcgtctcggc ggtcattacg
     7861 gtgaaccggg tatgctactt tttgtctacg ggcgagaaga aggcaatgat acccggcctt
     7921 cccgttatcc ggcgcgacag actcgcgaag ccagcgaggc ggtggcaagg cttaatcagg
     7981 tgaatcccca acaggtgatt ttcgcccagc aaaacccgga cgttatcgac cagagcgttt
     8041 ttcataatga cgtgattgcc gtgagtaacc gccaggtgct gttttgccac caacaggcgt
     8101 tcgctcgcca gtcacagtta ctggcaaacc tgcgggcgcg ggtgaacggt tttatggcga
     8161 tagaagttcc ggcaactcag gtttccgtgt cggatgcggt gtctacctat ctgtttaaca
     8221 gccaactgtt gagccgcgat gatggttcca tgatgttggt gctgcctcag gagtgtcggg
     8281 aacacgccgg agtatggggt tatctcaatg aactccttgc cgctgacaac ccgattagcg
     8341 aactaaaagt ctttgactta cgtgaaagca tggcgaatgg cggcggcccg gcgtgcctgc
     8401 ggttgcgggt ggtattgaca gaagaagaac gccgggcggt gaatccggcg gtgatgatga
     8461 acgatacctt gtttaatgcg ctcaatgact gggtggatcg ttactaccgc gatcgtctta
     8521 ctgctgccga tctggccgac ccgcaattgc tgcgcgaagg gcgggaagca ctggatgtat
     8581 tgagccaat
//
LOCUS       DAJTRQ010000147         8453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000147 DAJTRQ010000000
VERSION     DAJTRQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..909)
                     /gene="ugpC"
                     /locus_tag="OO332_003568"
     CDS             complement(<1..909)
                     /gene="ugpC"
                     /locus_tag="OO332_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR5943896.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKIS"
     gene            complement(923..1582)
                     /gene="pgmB"
                     /locus_tag="OO332_003569"
     CDS             complement(923..1582)
                     /gene="pgmB"
                     /locus_tag="OO332_003569"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5943897.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1579..3846)
                     /gene="ycjT"
                     /locus_tag="OO332_003570"
     CDS             complement(1579..3846)
                     /gene="ycjT"
                     /locus_tag="OO332_003570"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5943898.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3843..4449)
                     /locus_tag="OO332_003571"
                     /pseudo
     CDS             complement(3843..4449)
                     /locus_tag="OO332_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(4497..5243)
                     /locus_tag="OO332_003572"
                     /pseudo
     CDS             complement(4497..5243)
                     /locus_tag="OO332_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5274..6116)
                     /locus_tag="OO332_003573"
     CDS             complement(5274..6116)
                     /locus_tag="OO332_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5943899.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6103..6984)
                     /locus_tag="OO332_003574"
     CDS             complement(6103..6984)
                     /locus_tag="OO332_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5943900.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTSFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7005..8288)
                     /locus_tag="OO332_003575"
                     /pseudo
     CDS             complement(7005..8288)
                     /locus_tag="OO332_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
BASE COUNT         2004 a         2369 c         2116 g         1964 t
ORIGIN      
        1 gctaattttg gcggaaatgt tattttcctc ttgcgcgtcc ggatgaatat cttccgggcg
       61 tattcccatc acgatgggct tatgcaaact ttcctgtgtt ttcagaaccg ctaatttctc
      121 ttcgagaatg gttaatttaa gcgtttccgt aacgaattta tcgccatcga tcgtgccgcg
      181 aataaaattc atcgctggtg atccaataaa tccggcaaca aacatattcg cgggttggtt
      241 ataaacggtt ttcggcgcac ctacttgctg aacaatcccg tctttcataa tcacaatccg
      301 cgtcgccatg gtcatcgctt cggtctgatc gtgggtcacg tagatcatgg tggtgttcag
      361 tttctgatgc agcttgctga tctctgcacg catttgtacg cgcagcttgg catcaaggtt
      421 agagagcggt tcatccatta aaaacacgcc cgcttcgcgc acaatcgccc gcccgagcgc
      481 cacacgctgg cgttgcccgc cggaaagcgc gcccggcttg cgtttcaggt actcacgcag
      541 gccgagaatt tgcgccgccc agttcacccg ctcatcaatc acctctttgg cgattttttg
      601 catcttcagg ccaaacgcca tgttgtcgta aaccgtcata tgcggataca acgcgtagtt
      661 ctggaacacc atcgcgatat tgcgtgcttt ggctggaacg tcattcattc gtttgccgtc
      721 gatcaacaga tcgccgccgc tgatctcctc aagcccggca atcatgcgca gggtggtcga
      781 tttaccgcag cccgacgggc cgacaaatac gatgaactct ttatcggcaa tttccaggtt
      841 gaagtccttc accacatgca cctggttatc gtagattttt tgaatatgtt gtaacgaaag
      901 ctgagccatg ttgattcctt tgctatacgt tttgccagaa ggccgataac cgcgaccagg
      961 tcagggagtc cgttgagggc aacagtaatt gcgccccggt taagcccgca ccgatcccca
     1021 ccgagcgcat accgctggcg ttaatggcgt caatgcccgc ctgcgcatct tcaatgccga
     1081 tacatgcctg cggcggcacg cccagcccgg cacaggcagc gagaaagatt tccggatccg
     1141 gtttcgagtt tttaagttgg gaagcatccg cgcagaaggt gaaaaactcg cgcagctcca
     1201 gcgccgctaa aatcgtcggc gcattcaggg agacggaagc cagccctacc ggaatctgct
     1261 gtgcacggag ctctgccagc aaattgcgaa taccgggcag cacagcatta accgtcagct
     1321 cgcgcagtga gtggacgtaa agcagatttt tgcgatacgc cagttgcgcc cgctcctgcg
     1381 ggttaaagtc gccctctttg cccccgtgtt gcagaatgcg ccgcagagac tcatcgcggc
     1441 tgatcccttt tagagattcg ttaaactgcg catcaatact gatgccaatt tcagcggcaa
     1501 tctgctgcca cgcctggaaa tgcagatgcg cggtatcggt gattacacca tccagatcga
     1561 aaattacccc ttgcagtttc attcatcctc ctgatgcgtg gtagcggtcc cattgaaggg
     1621 caaaataaaa tcacccaaac agaaaacaga ttcttcagcc acggatataa gctgaccgtt
     1681 caaacgcagt gaaacgggcg cagaagttcg aatcgcaata cgctgcgcgt cgagagtgac
     1741 ctgtaattcg cagccctgcc agaacagagg gaaagacaac tgttgccact gctcaggtaa
     1801 cgccggattt agatgcaatt caccgtcacg cacgctcacc ccggcaaaac cctgaatcgc
     1861 ccccagccag atagcgccag ttgcggcagc atggatacca tcatcacaac tatgcggatc
     1921 agcaccaaga tcgatttcag tcccctcgcg ccagaactga taactttggg tcagcaggcc
     1981 acagcgtgca gcaacaatgc cgtggattgc tttacttaat gacgagtcgt gaatagtgcg
     2041 cggttcataa aattgcagat tggcaagaca cgatgccgat gagaactgct ctggcagcat
     2101 gtaattgagc atcaccacat cagcttgttt gaggatctgc atctcgttca cttctgcgcg
     2161 tgaataatcc agcagaatgg tttgcttccc cgccgccgct ttgtatttcg ccagattaat
     2221 cgccggctta gtcataaacg aatcatcctg cggcaaaaca ccgtcgggct gaatttctgg
     2281 cagccgcagt tctttaagga acatttcggc acgatgaata aacgcgtcgt cactacagcc
     2341 aaactggcgg gcaatactca gcgcctgctg gacgttgtaa tacaccatat agctggtgaa
     2401 ggcgttatta ttgacatgtt cggtatattc gtctggccca ataacatcat gaatttccag
     2461 acggtcgtta accctcaccg cgcggctaat ccagaacttt gcagtttcca gaagtagcgc
     2521 catgccttca tgcgcaatga aactttcatc ccccgtggtc tgccagtatt gaataaccgc
     2581 ccaggcgata tcggccacca gatgatgttc cgcctgcgcc gaggccactt tttgccgcag
     2641 cccggtgcga atgttaatgg cggcaaattc cggcgtctct tcttcgccgc tgcgcgcgct
     2701 ttcccacgga aatagcgcgc cctgccagcc gttgcgtcgc gctttctcct gcgcgcctgg
     2761 caagttgtgc cagcgataac gcagtaaact tcgggcaacc gtcggatcgc taaacagatg
     2821 gaacggcagc aaaaatactt ctgtatccca gaaaacgtgg cctttgtagc cttcgccggt
     2881 taagcctttt gccgcaatac tgctgcgctc gccgtgagcc ggcgtcatga tgcgcagatg
     2941 ataaagcgca taatctaacg cttgctgatc gtgcgcatcg ccgccattta ccgtgatacg
     3001 acgtttctgc caccattgac gccagttttc tgttgatgct gcaagatgtt ggtcgtaact
     3061 ctgttgcgcg cacatttcaa gctggcgaag cgacacgctg ccccactcgt ctaaagcagc
     3121 ttgcctgtca tcccgccagt cgatccacac cagtttttgc aacgtcactg tctcgcctgc
     3181 atgaagctgc gcactggtat gttgcagtaa acggcgctct ttggcggtat agcattgctg
     3241 cacatcaccg ctcacctggc agcaacaact gatggccaca tcactgcgcc catcctgggt
     3301 ggtgtagatc ccctgcatca aatgctgacc aaacacccgc acctgggttt cgtcgagatg
     3361 ctggcgaccg tggttggttt gcgtggcgtc aatgcctgtt gaaatcagca ctgacgcgtc
     3421 ggcgtccagt ggcataatag tgatttccag cgcaatgagc ggcagttggt ctgccgaaac
     3481 aaagcgacgg ctggcgatgg tgtaacctgc gccgttgctg gtacgccaga caacgctacg
     3541 gcgtaattct ccactggcaa agtcaagttc acgctgccag gcttcgcggg ataacgagaa
     3601 aacctcacca tttatggcaa tctccatccc caagatatca ggcagattca ccagttcgtt
     3661 gatttcacct tttcccgccc gatgatacag ccccgccaga tacatccctc gcgcctggcg
     3721 cgtgtaatct tcttcatggc tggcgcgaag cccaagatag ccgttcccct gcgccatcag
     3781 cgatgcatac ttgttcaggg tatgctggct gaaatggggt tctgataacg ttactggcct
     3841 ggtcataatt ccacacgcgt ccctgtttct gcggattgat ataacgccgc caccagttgc
     3901 tggatgatgt acccctgctc ggcgtcggct atcatcacgg gcttgccctg tacgtgattg
     3961 ataaaggctt ccatgctgcg caaatggcgg ttgtcgtctg ccatttcccg ttgcatcagc
     4021 gtcattaatt caccgttgtt atcggtgtag atatgtgctg gaaacagcgt cgcaccggct
     4081 ttatcaccac aaaagctgac gttcataatc aactgttcgc ggatgttgag tgcaaatgac
     4141 gtttccagcc acagaatgcc gccgttatga aattcaatgg tgccaaacag cgaatcttcg
     4201 acgctgtaag ttgccggatc ccactcgcca aattgaccac agctcttttg cgtgccgatc
     4261 ttttgaaaac tatgcgcatt cacgcttttc accgccggaa agcccagcac atacatcgca
     4321 gcatccagca tatgaatgcc gatgtcgatc cagttcttta ttggtaaaga caccccagcc
     4381 gggaacgccg cagcgacgca gggcgcgggc ggtggtaacg taaatctctc ccaaaacgcc
     4441 gttggtcacc tgttcacgca gctgttgcgt atcgagcgca aacgatggtg aaagtcgtag
     4501 gccaggccgc gcagcgccga ttgcagcgcg tcggcgtaac cgctggtttc gataatcaca
     4561 tcggccccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt gccaatgggg
     4621 ttaaggcaga aatcagcacc gtggcgacgg gcaatatcac agcgatgggc gataggatcg
     4681 acgccaatta ccaccgatgc gccagcgcgt ttcgccagtt ggatggcaat ttgaccgatc
     4741 gcgccaagcc ctaccaccac gacaaaatcc cctacgcgta cgttggcatc gcgcacgccg
     4801 ctcatggcaa actgcgccgg gtcgtaacag acggcgtttt tccaggagct gccttgcggc
     4861 attttgcgca gcttgtagtt attcactgcg ttaatgatga ccgtctcgga gagcgggccg
     4921 tagccgcata cgctgtcgcc caccgcgtag tctgtaacgt cgctgccgca ctcgataatg
     4981 tcgccaacca ccatgttgcc aagctggaat ttgccaaact caatgccgcg cggcgcatct
     5041 gccggacgcg gggtgaacat ctgccactcg ccgttaaagt cttcatcgat aaacgggctg
     5101 gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg cacgcggatc
     5161 ttcacttcat tggctaaaat cgcccgatct tcatactcaa ccagcgcagc aacacgcggt
     5221 gctgtggcta ctaacttttt catgtaaaac tccttgtaaa gaaactggcc gctttagccc
     5281 ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc atacataatc
     5341 accaccggca atgcggtcac cagcgaggcc gccatcatcc gtccccagat gtaatcaggc
     5401 gtactgaaca gcgcgttcag gcccactggt aaggtgaaat tgctggcgct ggagaggaaa
     5461 atcgacgcaa acagatagtc gttccacgtc accataaagc agtagacgaa caccgatatc
     5521 agcccggaca tcgccagggg cacggtgata cggaagatga tttgcagacg gttgaggcca
     5581 tccatcatcg ccgcttcttc gatctcatca gggatggtgt cgaagtaact tttcagcata
     5641 aacacggcgg tgggcagggt ttgcgtcacc atggtaatga tcagcgccat ctcggtgtcg
     5701 taaataccaa gcgcggtgat gattttgaac agaggcacca ccagcaaaat gccagagaac
     5761 atatacaccg tgtaaaagct ggcgttgatg gtcatccggc ctttaaagcg caggcgggaa
     5821 agcgcgtaag ctccgagaat gccgagaaat accgccacca cggaggaaac caccgacacc
     5881 accatgctgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa aatgtcgacg
     5941 taatgctcca gcgtccactg ttgcggcagc agcgtaggat gcagcgatat cgcctctttc
     6001 gcgcttttga acgaggtcat cagcatcaca aaaaacggaa acagcgtgat gatgagaaac
     6061 agcgccagcc cgcagtaaaa accgatacga ctgagcgtgc gtttatttgt tgccattgag
     6121 gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga gcaccaccga
     6181 gatcgccgcc gctttcccta agtcattaaa ggcaaaggag gttttgtaga gatagacacc
     6241 aagaatatcg accttggttg tcagcagata aacatcagcg aacatgtaga acatccagat
     6301 ggtgcgcagg gtcaccaccg tcgccaagac cggcataatt gcgggcagcg tgacgatgcg
     6361 aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg atttgtcgat
     6421 ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc agatggcgag
     6481 cagcaccacc agcacaaagc tactgcccgg attatcaaac cacagcggtg cctgctcata
     6541 gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt tgaacatgta
     6601 tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca ccagcgaacg
     6661 cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccacggcca gcccgagcac
     6721 ggtgctgccc gccaccacca gcgccgtata ccagaccgtc atccacagcg agtgccagaa
     6781 gccgggatcg gagagaatgc gcacatagtt actcacccca acaaacgttg actcgatgtt
     6841 gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccatcg gccacgccac
     6901 cagaccgccc agcagtaata agctgggcgc gagaagcagc agcgcaaacg gcatatcgga
     6961 acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg ctgttcgatc
     7021 agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc tttacccacg
     7081 gtgacgttat gcaccatgga gctcaccaca ccagaacccg tcacatcgcc catgcgggta
     7141 aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc gattagctga
     7201 ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaagtggc ggtagtgaca
     7261 accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc ggcaatgttg
     7321 tccgcctgct ccataaaggt gacaaatttt tctgctgctt cggtcttttg tccggcggta
     7381 atggtcagcg aggtcaacat gccgtagacc gcagagtttt tctcggttgg caccacgaaa
     7441 ccgacgtttt tcgggtcgcc ttctttaatc acagccggaa ggatataggt ggagtaaatc
     7501 gccatcggcg cggtgccgtt cataaaggcg tctttcactt ccatgatgtc gttagaaccc
     7561 ggcatggtgt tggcagcaag gtcgcggtaa taggtcagcg cctgcatcat ctctggtgta
     7621 tcaagggtga ttttgccttc ggcgttaaag acgttggcct agttggataa cgcaaactgg
     7681 gagaaggatt gttccgtcag cacgctttct gccgtaggca gcgcaatacc gtatttttta
     7741 ttcgccgggt cattcagttt ctgtgcaacg tccagcagct gttgccagtt tttcggctcc
     7801 tccagccccg cttttgccag cacatctttg cgataccaga tgccgccaat ccaggcgctg
     7861 acaggaacac cggtccatgc gctaccatct tcggtacgca caatacgcag tacgccatcg
     7921 taaaacgcgc cttcaccaac gttgctgatg accgtggcaa ccgctttgcg atcgataagc
     7981 tgctctttat ccataacttt ggcgtagtca tggctggttt cgatcacttc cggcagcgag
     8041 ccgctacgtg aaagggtgat gactttggtg ttataagcgt cttcttccac gggcacttgt
     8101 ttaacggtga tacctgggtt ttctttttca aaacgggcaa tcaatttgct gataactgcc
     8161 tgacgctcct gctccaccga agaatgcata aattcgatgg atacatgggt tttattttct
     8221 tctttacagc ctgaaatcag ggcgcaggaa accagtgctg ataacagcac gatttttgat
     8281 ttaatcatga gatgttcctt tttatttaat ccacataacc tgccaagggc gcagcgttat
     8341 ttcattaccc aatatatcaa cttcactaat cagatcccga ccatgcagat cagtaatatt
     8401 tacatgctga atatttttac tgacattaaa caatccagtc agacaattac cat
//
LOCUS       DAJTRQ010000148         8349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000148 DAJTRQ010000000
VERSION     DAJTRQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            478..1452
                     /gene="hemB"
                     /locus_tag="OO332_003576"
     CDS             478..1452
                     /gene="hemB"
                     /locus_tag="OO332_003576"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5943901.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1558..2409)
                     /gene="tauD"
                     /locus_tag="OO332_003577"
                     /pseudo
     CDS             complement(1558..2409)
                     /gene="tauD"
                     /locus_tag="OO332_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2394..3233)
                     /gene="tauC"
                     /locus_tag="OO332_003578"
     CDS             complement(2394..3233)
                     /gene="tauC"
                     /locus_tag="OO332_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5943902.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3230..3997)
                     /gene="tauB"
                     /locus_tag="OO332_003579"
     CDS             complement(3230..3997)
                     /gene="tauB"
                     /locus_tag="OO332_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5943903.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4010..4936)
                     /gene="tauA"
                     /locus_tag="OO332_003580"
     CDS             complement(4010..4936)
                     /gene="tauA"
                     /locus_tag="OO332_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5943904.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5203..5718)
                     /locus_tag="OO332_003581"
     CDS             complement(5203..5718)
                     /locus_tag="OO332_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943905.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            5911..6162
                     /locus_tag="OO332_003582"
     CDS             5911..6162
                     /locus_tag="OO332_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5943906.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            6164..8257
                     /locus_tag="OO332_003583"
     CDS             6164..8257
                     /locus_tag="OO332_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5943907.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
BASE COUNT         2113 a         2177 c         2104 g         1955 t
ORIGIN      
        1 atgcatagaa aacatattat gtttcgcgct aacgattatg ctgcgttaca gacgaatggt
       61 tcaattattc tttcccctaa gtccgaatgt ggacatatat aatttatgga gaaaaacacg
      121 catcaaattg ataaaaggac gatatttcag catgttgcag ataatgttat ttttgatgca
      181 tacgcgttat aaatatcaaa aacacaaacc agataaatat cttatcaata tacaacaaag
      241 tttgaattat tcaatttata gcagcacaac caaacgccaa aaaaataata accaagagct
      301 ataaataaag attaaaaata ttttcagtga atatgaaatt attgctattt ctattacaaa
      361 gatgtcctgg tctctctttc attctcttct tgtcaacatc gcgacaactt tcgtaaaaca
      421 ctcctgcccc gcttcaggta tactgtgcac cttaattcca caaacatcag gcagaccatg
      481 acagacttaa tccaacgccc tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt
      541 gaagagacaa cacttagcct taacgacctg gtgttgccga tctttgttga agaagaaatt
      601 gacgactaca aagccgttga agccatgcca ggcgtgatgc gcattccaga gaaacatctg
      661 gcacgcgaaa ttgaacgcat cgccaacgcc ggtatccgtt ccgtaatgac ttttggcatc
      721 tctcaccata ccgatgaaac cggcagcgac gcctggcggg aagatggact ggtggcgcgt
      781 atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc
      841 tgtgaataca cttctcacgg tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac
      901 gcgactctgg aaaatttagg caagcaagcc gtggttgcag ctgctgcagg cgcagacttc
      961 atcgcccctt ccgccgcgat ggacggccag gtacaggcga ttcgtcaggc gctggacgct
     1021 gcaggcttta aagatacggc gattatgtcg tattcgacca agttcgcctc ttccttttat
     1081 ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg
     1141 aacccaatga accgtcgcga ggcgattcgt gagtcactgc tggatgaagc ccagggcgca
     1201 gactgcctga tggttaaacc tgctggagcg tacctcgaca tcgtgcgtga gctgcgtgaa
     1261 cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg agtacgcgat gattaagttc
     1321 gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc tcgaaagctt aggttcaatt
     1381 aagcgtgcgg gtgcggatct gattttcagc tactttgcgc tggatttggc tgagaagaag
     1441 attctgcgtt aatttttttc ctctcagaaa gggggagtta acctgttgaa agtacggtga
     1501 acgccccctc accctaaccc tctccccaag ggggcgaggg aaccgtccac actcgtatca
     1561 ccccgcccga taaaacggtt tatccccaag gatcgtcgcc cgatgcataa tccgccgctg
     1621 tggcaggtaa tcggcattgg catagtgctg agtcacgcgg ttatcccaaa tcgcaatatc
     1681 attcggttgc cagcgccaac gcacctgaaa ctccggttta gtgatatggg cgaataaaaa
     1741 gcctaacaag gcttcgctct ctttctcgct gacatcgaca attcgcgtag taaagccttc
     1801 attcacaaac agcgcctgtt taccgctcac cggatgcgtt cgcaccaccg gatgtagcaa
     1861 cggaggattt ttcgcgaccg cctcgcgcca gcgttgatgt tcctcctcgg ttttgcggta
     1921 tttgtattcc gggaatgatt tacggaaatc atgctccgca cgcagccagc tcagcagctg
     1981 gcggaaggga acagagagcg cctcataggc cgcaataccg ctggttcaga gcatatcacc
     2041 gccggtcgaa ggtaactctt tagctgccag aatcgcccct gcgggtggcg tttcaataaa
     2101 tgtcacatcg gtatgccagt tgtcgttatc cggcggatta tcgttatggg tatccagcac
     2161 gatgatctcg tcaacccctt cggcatgagg gtaaacaggg tgaatatgca gttcgccaaa
     2221 acgctgggcc agcgcgcgtt gctgctgcgg cgtaatagct tgatcgcgca gaaataccac
     2281 ctgatggcgc agcaccgcat ggtaaagctg ttcaaactga ttatcgctta acgggcgcgt
     2341 caggtcggca cccgaaattt gtgcgccgat atgcggcccc agcggggtaa tgctcagacg
     2401 ttcacccatt gtacttctcc atgccagggc gtcagacggc gctgtaacgc gcgcagaccc
     2461 agttctaaaa gaaaggcgat aatcgcaatc accgcgatcc ccgccagtac cacgtcagtc
     2521 gcgagaaatt cacccgctga ctgaaccata aatcctaaac cgcgtgtcac ggcaatcagc
     2581 tccgccgcta ccagcgtaga ccagcccaca cccagaccaa tgcgtaatcc ggtgaggatt
     2641 tccggcagcg cgccgggcaa aatgacaaac cacagcacct gcgcacggct ggcacccagc
     2701 gactgggcgg cacgaatgcg aacctgctgc gcgcttttca cccctgccag cgccgacatc
     2761 gccaccggcg caaaaatcgc taaatagatc agtaagatct tcgaggtttc accaatacca
     2821 aaccagatca ccatcagcgg caaataagcc agcggtggca ccggacgata aagttcgatt
     2881 accggatcca gaatgccgcg caccgtcggg cttagcccca tggcgatccc gaccggaata
     2941 ccgatcacca ccgccgcgag tagcgcctgc acaatgcgcg tcaggctggc agccagatgc
     3001 tgccacagcg tggcgtccat aaatccctgc ggtccggcaa tggtgagcag ttttgccagt
     3061 acttgttgcg gcggcggcaa aaacagcggg ctaatcagtt gcagcgccgc caccgtccac
     3121 catacagtga gtaaaaccgc taacgtgcca atgcttaagg tcacctgacg cgagagcggc
     3181 cagcgccatt tcagccgccg cgaatgcagt ttttcattaa tgagcacact catgagaacg
     3241 cctcccgttg ctcaaatacg cggcttaaaa catattcgcg catggcgatg aattgtggat
     3301 cggacttgat gctgcggctc gactctcccg caacaaagcg gcgagcaaag ttgagcggca
     3361 gccgctccag cacacggcca gggccggatg aaagcagaac cagttcagtc gccataaaca
     3421 ccgcttcttc tatatcgtgg gtaatcaaca gcacctgctt gcccgtctcc tgccagagtt
     3481 tcagcagcag agtttgcatc tggtcgcggg tgaaggcgtc cagcgcacca aacggttcat
     3541 cgagtaataa cagctgggga ttcgccgcca gcgcgcgggc aatccccacc cgctgacgtt
     3601 gaccatcgga aagctgccag atatagcgtt tttctgcgcc ttccagcccc acttttttca
     3661 gcatctggtg cgcgatttcc agtcgctgca ttttctctat acctgccaat tgcaggccga
     3721 acgccacgtt gtcctgtaca ttgcgccacg gtagcagccc ttcattctga aaaactacgc
     3781 cacgctctgc tcccggtccc tcaatacgct tacccgccag ttgaatgctg ccatgctgat
     3841 aaggcacaaa accggcaatc agattcagca gggtggtttt accgcagccg gacggcccca
     3901 gcaccaccag tagctcgccg ctttccagcg tcaggttgat atcttccagt gccggtttgc
     3961 cgccataatc ggcatagaga tgagagattt gcagcatctg cgcctccttt tattgcacga
     4021 agcgcgaggt aacgtactgg ctgtaatcat tcgctacagc cgggaccttg ccctgctctt
     4081 tcaaaaactg cgcggtgtcg atgattgctt tgttcaccgg tccggtcagt tctgccgttt
     4141 gttgctgcgg cgtcagatag gtatttccct tcaccagccc cggaacgtca ccttcaggca
     4201 cgccgcttaa acgcgccagt ttgctgatgt tttccggctg tttcagccac gcgtcagggt
     4261 tagcaatgta cggttgctgt gcatcgatgg cgcttttggc gaacgctttc acgacctcag
     4321 gatgtttctc ggcaaaatct ttgcgcacca cccagacgtc cagcgttggc gcaccccact
     4381 gcccgacctg ttcagaatcg gtcagcacct tgtcgtcttt ttccagggcg ttaaccgccg
     4441 gtgcccagac ataagcacca tcaatatcac cccgctgcca tgcggcgata atcgcgggcg
     4501 gctgcaggtt cacaatctcc actagccctg gtttaatccc ccagtgtttc agcgccgcca
     4561 gcaggctgta gtgggtggtg gagataaacg gtacggcaat gcgtttacca atcaaatctt
     4621 cgggtttgct gatagttttc tttaccacca gcgcttcgga gttacccagt tttgacgcca
     4681 gcaagaagac ttcaatcggc acctgttggc tggctgcaac cgctaatggg ctggatccga
     4741 ggttgccgat ttgcacgtcg ccagaagcca gcgcccgcac gatgctggct ccgctgtcaa
     4801 acttacgcca gtccacgctc gccccgctct ctttagcaaa ggtgttgtcg gcctgagcca
     4861 ctttcgccgg ttcggctgag gtttgatacg ccacggtgac gttcaccgcc tgtgcctgaa
     4921 aagcgatgaa tgccagtgcg gcaagaagtg tgttatatga ctaggtaatt tattcaagat
     4981 aaccgggaat gggtaaaaaa agacgggatt tgatgggatt caataagtta tgcgccgatt
     5041 gcattcaaga taaaacgagt taaaaatgct caatgcttcg atatcgataa aacattcaag
     5101 ataggcgcgc cgtgtatttt aattctttta agactcttca aaaatgtcat cgtagccttt
     5161 aaaaacgaat tttaaaggca ccctaaaggc aggtcaatga agttaacccg ccttcgcgtt
     5221 tacactctct gagagaatac tagcttcaat ctcttccaaa atctctctga cttgctcgtc
     5281 gggcatatca acgagcatca acgctgccag gcgtttacgg ccctctagtt gcagtagctc
     5341 ttgagtttcc ttatctaaca agcgtagcac agtctcaaca cctttccttg cgagcatgtt
     5401 tgtaagggct tctcgttcct cactggtaag aaacgacaac atttctgtta gctgatcccc
     5461 ttggcttaaa tcactttgtg ttttttgtgt ttttggtgga ttcccgttac cggtttttag
     5521 ccattcaaga ctaacacctt cactttctgc aatgcgtacc aatatgcttt ctcgaggttc
     5581 tggtgtattt ccctttttaa aatagctttg taacgtattg atatttatgc cacaggctct
     5641 ggcggcagca cttcttgatg gataccgcct aataagataa gccatgcgct ccacgatagg
     5701 ttcttttgcc tgctccataa aagtacctct aggtattttt ctgtataaag gttcttttcc
     5761 atctatctaa ttgaatggaa aggtaatttt aaaaatccaa ataaaataca aaaaaatacc
     5821 ttgtattggt gctttttgtg atctgcgatt ttatttactc ggatatccac gcgaatatcc
     5881 gcgcgggtaa ttttgtaggg taaaggaaag atgagacaaa gagaagatag tggccgcgat
     5941 tggccttcgc tgatgattca gcaagcactc aaagataatg ggtggtcttc ctttcgggag
     6001 ctttcactcc atgccggtta tgctcaggat tcactgaaag cggtagttcg tacgccaaac
     6061 aaaaaatacg aacgtgttat ttctgacgct ataggtgtac cgccagaaat catctggccc
     6121 agccggtatc gtaacgatgg atttatcagg aaggcgtcgt gatatgcagt cgtacgttac
     6181 cgtaaatgac cttctcggtg ttcctggaat gccagccacc gcgaaaggca tccgacaggc
     6241 gttacagcgt ttttctcgtg ctcttggtga cgttagtcgc cgtcgagcag gtacaaaagc
     6301 cattgagtac catatcgatt gcctgcctga ggtaacccaa aaggcactgc gtgaacgcta
     6361 tgtggaacaa ctggtggcta cagagaacaa cgtctccgaa gtgaagaccg ttaccaggac
     6421 ggcgcgtaac cctgatgctg ttcaggcagt tgaagcatat cggggttctc cgcaactaat
     6481 ggaagaacgc ctgaatgccc tgactgaaaa tcagcgccgg gtgtctgaag caagagcggc
     6541 gctggcagtg gaagtgctga agctggaaag cgccggtaac ctcgggcgac tgaaagccat
     6601 taactttctg gttgaaaaag cccgtaaagg tgagttgccg gaacgactgc aacaggctgc
     6661 gattaacgct aatgcaaaac gtggcactaa tcgcaccatc agccgtgatc cgctttacca
     6721 gtgggtactg aaatacaacc ggtcgcagaa cgccgcagaa cgactgttgt tactggcacc
     6781 aggaaaacgc gacgaaataa aaccggaaga aattagctgg ctgcctgaat ttctggcgca
     6841 gtatcgtcag gctaacggca gacctatgtc ggaagcctac gaggattttg tcacggaatg
     6901 gcaacgtcgc tatgcggacg aaccgtacat gctggaggtg atgccttcct atgatgttgt
     6961 ccggtatgcc atgaaaaaat tgccggaagt ggtgaaacaa aaaggacgcg ttaccggcag
     7021 tgaataccgc cagcttgagg gattcacccg ccgcgactgg acagctatgc cggtgaatta
     7081 tgtctggatt ggtgacggcc acggtatgaa gctgaaatgt gcgcatccga tccacgggcg
     7141 tccattttca ccggaagtca cgtttgttat cgacggtgga acccggtttg ttgttggctg
     7201 gagtcttgat cttgcggaga acgtgtttgc tgttgccggg gcgatacagc acggaattcg
     7261 caatcacgga aaacccttcc tgtattactc ggataatggt tccggtgaaa cggcagatat
     7321 gctggataaa gagatcgtcg ggattctgcc ccgtctgggt attaaccacc ccaccggtat
     7381 tgccggaaat ccgcagggac gcggcatcat tgaacgtctg aaccggacgc tgccgatgcg
     7441 tatagccaga cggtatcgca cctattttgg caagggggca gaccgggaaa cactgcgtgt
     7501 actcaatcgc gatctgcgtt cagcatttaa tgccctgcaa caggacaaac cgctgaatgc
     7561 ccgccagaaa tctgcaatgc gcgagctgcc gtcctggtca gaactgattg aggctattcg
     7621 tgaaggagtg gaatggtaca acaaccgccc gcattctgaa ctgccgatga aaccaaacgg
     7681 gaagcattac agcccggctg agttcagaaa aaaacgcctg gcggaagaaa acaccgaaat
     7741 tgaatggctg agtgatctcg aactgcgcga catgttctgc ccgatggtgg agcgtccggt
     7801 aagacgctgt gaaatcaaat ggcttgggaa tatttattac gcgccggagc tgcgtgatga
     7861 acatggtcgc aaagtgctta tcagctatga cattcatgat gcagagcgaa ttacagtgcg
     7921 ccgtaaggat ggcagtttta tttgtgaggc gatatggaac ggcaataaac gcgccgcctt
     7981 cccggtcagc gccgactatt acaaacaaca gcagcgtatt aaaggaatgc acaaacgcgc
     8041 agaagaaaaa atccgtgatg ccgtggatga aggtattcag gttattgaac aaaaaacgac
     8101 agaaccctgg ctggataatg tttatcgacc cgttggcaat gtgatgaccg ttcagcaaca
     8161 ggaatatgaa gaggagcacg acgaagaatc cgaacgcaat ttcaggcggg gattacagaa
     8221 actcattgcc atgcaggacg atgacccact ggcctgaata aaaaaacagt ccgcagcaac
     8281 ggactgttaa acaggtaaat aaagctgatt tgaaataacc gaggaaacaa tatgaccgat
     8341 attaatgat
//
LOCUS       DAJTRQ010000149         8344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000149 DAJTRQ010000000
VERSION     DAJTRQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO332_003584"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO332_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5943908.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(964..1437)
                     /locus_tag="OO332_003585"
     CDS             complement(964..1437)
                     /locus_tag="OO332_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5943909.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1597..2610
                     /gene="intI1"
                     /locus_tag="OO332_003586"
     CDS             1597..2610
                     /gene="intI1"
                     /locus_tag="OO332_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5943910.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2813..3163
                     /locus_tag="OO332_003587"
     CDS             2813..3163
                     /locus_tag="OO332_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5943911.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3289..3849
                     /locus_tag="OO332_003588"
     CDS             3289..3849
                     /locus_tag="OO332_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5943912.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3852..6818
                     /locus_tag="OO332_003589"
     CDS             3852..6818
                     /locus_tag="OO332_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5943913.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6885..7262
                     /locus_tag="OO332_003590"
     CDS             6885..7262
                     /locus_tag="OO332_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5943914.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7463..8122)
                     /locus_tag="OO332_003591"
     CDS             complement(7463..8122)
                     /locus_tag="OO332_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5943915.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1841 a         2434 c         2270 g         1799 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa cgcctggcac agcggatcgc
      901 aaacctggcg cggcttttgg tacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac
      961 ttgttaaccc ttttgccaga tttggtaact ataatttatg ttagaggcga agtcttgggt
     1021 aaaaactggc ctaaaattgc tggggatttc aggaaagtaa acatcacctt ccggctcgat
     1081 gtctattgta gatatatgta gtgtatctac ttgatcgatc aggcttttgt atatctcccc
     1141 accacctgaa acaatgacat gatccgttat tttctttagg ttggttaaag catctttaat
     1201 tgatggaaag atcactacgt tctcattgtc agatgtaaaa cttgaacgtg ttacgaccgc
     1261 atactttcgg ttgggtaatg ctcccattga ttcaaaagtc ttgcgtccaa ccaacagcca
     1321 ttggttatag gtaatagctt taaacaggag ctgttcacct ttggcactcc atggaatatc
     1381 agggccattc ccgataactc cattcttcga tatagctacc attagtgata gtttcacaat
     1441 tcttcctcag aggttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1501 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1561 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     1621 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     1681 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     1741 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     1801 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     1861 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     1921 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     1981 ctggaaggcg agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt
     2041 gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg
     2101 gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg
     2161 cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg accaggccga gggccgcagc
     2221 ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca ttcctggccg
     2281 tggttctggg tttttgcgca gcacacgcat tcgaccgatc cacggagcgg tgtcgtgcgt
     2341 cgccatcaca tgtatgacca gacctttcag cgcgccttca aacgtgccgt agaacaagca
     2401 ggcatcacga agcccgccac accgcacacc ctccgccact cgttcgcgac ggccttgctc
     2461 cgcagcggtt acgacattcg aaccgtgcag gatctgctcg gccattccga cgtctctacg
     2521 acgatgattt acacgcatgt gctgaaagtt ggcggtgccg gagtgcgctc accgcttgat
     2581 gcgctgccgc ccctcactag tgagaggtag ggcagcgcaa gtcaatcctg gcggattcac
     2641 tacccctgcg cgaaggccat cggtgccgca tcgaacggcc ggttgcggaa agtcctccct
     2701 gcgtccgctg atggccggca gcagcccgtc gttgcctgat ggatccaacc cctccgctgc
     2761 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga caatggaggt
     2821 ggtagccgag ggtgtggaaa cacccgactg ccttgcgtgg ttgcggcagg cgggttgcga
     2881 cacggtgcag ggtttcctgt tcgccaggcc gatgccggcg gcggccttcg tcggcttcgt
     2941 caaccaatgg aggaacacca ccatgaacgc caatgaaccg agcaccagtt gctgcgtgtg
     3001 ctgcaaggaa atcccgctcg atgccgcctt cacgccggaa ggggccgagt acgtggagca
     3061 tttctgcggg ctggagtgct atcagcgctt ccaggcgcgg gccagcactg cgaccgaaac
     3121 cagcgtcaaa ccggacgctt gtgattcgcc gccgtcaggt tgaggcatac cctaacctga
     3181 tgtcagatgc catgtgtaaa ttgcgtcagg ataggattga attttgaatt tattgacata
     3241 tctcgttgaa ggtcatagag tcttccctga cattttgcag ggaattccat gactggacag
     3301 cgcattgggt atatcagggt cagcaccttc gaccagaacc cggaacggca actggaaggc
     3361 gtcaaggttg atcgcgcttt tagcgacaag gcatccggca aggatgtcaa gcgtccgcaa
     3421 ctggaagcgc tgataagctt cgcccgcacc ggcgacaccg tggtggtgca tagcatggat
     3481 cgcctggcgc gcaatctcga tgatttgcgc cggatcgtgc aaacgctgac acaacgcggc
     3541 gtgcatatcg aattcgtcaa ggaacacctc agttttactg gcgaagactc tccgatggcg
     3601 aacctgatgc tctcggtgat gggcgcgttc gccgagttcg agcgcgccct gatccgcgag
     3661 cgtcagcgcg agggtattgc gctcgccaag caacgcgggg cttaccgtgg caggaagaaa
     3721 tccctgtcgt ctgagcgtat tgccgaactg cgccaacgtg tcgaggctgg cgagcaaaag
     3781 accaagcttg ctcgtgaatt cggaatcagt cgcgaaaccc tgtatcaata cttgagaacg
     3841 gatcagtaaa tatgccacgt cgttccatcc tgtccgccgc cgagcgggaa agcctgctgg
     3901 cgttgccgga ctccaaggac gacctgatcc gacattacac attcaacgat accgacctct
     3961 cgatcatccg acagcggcgc gggccagcca atcggctggg cttcgcggtg cagctctgtt
     4021 acctgcgctt tcccggcgtc atcctgggcg tcgatgaact accgttcccg cccttgttga
     4081 agctggtcgc cgaccagctc aaggtcggcg tcgaaagctg gaacgagtac ggccagcggg
     4141 agcagacccg gcgcgagcac ctgagcgagc tgcaaaccgt gttcggtttc cggcccttca
     4201 ccatgagcca ttaccggcag gccgtccaga tgctgaccga gctggcgatg caaaccgaca
     4261 aaggcatcgt gctggccagc gccttgatcg ggcacctgcg gcggcagtcg gtcattctgc
     4321 ccgccctcaa cgccgtcgag cgggcgagtg ccgaggcgat cacccgtgct aaccggcgca
     4381 tctacgacgc cttggccgaa ccactggcgg acgcgcatcg ccgccgcctc gacgatctgc
     4441 tcaagcgccg ggacaacggc aagacgacct ggttggcttg gttgcgccag tctccggcca
     4501 agccaaattc gcggcatatg ctggaacaca tcgaacgcct caaggcatgg caggcactcg
     4561 atctgcctac cggcatcgag cggctggttc accagaaccg cctgctcaag attgcccgcg
     4621 agggcggcca gatgacaccc gccgacctgg ccaaattcga gccgcaacgg cgctacgcca
     4681 ctctcgtggc gctggccacc gagggcatgg ccaccgtcac cgacgaaatc atcgacctgc
     4741 acgaccgcat cctgggtaag ctgtttaacg ctgccaagaa taagcatcag cagcagttcc
     4801 aggcgtcagg caaggccatc aacgccaagg tacgtctgta cgggcgcatc ggtcaggcgc
     4861 tgatcgacgc caagcaatca ggccgcgatg cgtttgccgc catcgaggcc gtcatgtcct
     4921 gggattcctt tgccgagagc gtcaccgagg cgcagaagct cgcgcaaccc gatgacttcg
     4981 atttcctgca tcgcatcggc gagagctacg ccaccctgcg ccgctatgca ccggaattcc
     5041 ttgccgtgct caagctgcgg gccgcgcccg ccgccaaaaa cgtgcttgat gccattgagg
     5101 tgctgcgcgg catgaacacc gacaacgccc gcaagctgcc agccgatgca ccgaccggct
     5161 tcatcaagcc gcgctggcag aaactggtga tgaccgacgc cggcatcgac cggcgctact
     5221 acgaactgtg cgcgctgtcc gagttgaaga actccctgcg ctcgggcgac atctgggtgc
     5281 agggttcacg ccagttcaag gacttcgagg actacctggt accgcccgag aagttcacca
     5341 gcctcaagca gtccagcgaa ttgccgctgg ccgtggccac cgactgcgaa caatatctgc
     5401 atgagcggct gacgctgctg gaagcacaac ttgccaccgt caaccgcatg gcggcagcca
     5461 acgacctgcc ggatgccatc atcaccgagt cgggcttgaa gatcacgccg ctggatgcgg
     5521 cggtgcccga caccgcgcag gcgctgatag accagacagc catggtcctg ccgcacgtca
     5581 agatcaccga actgctgctc gaagtcgatg agtggacggg cttcacccgg cacttcacgc
     5641 acttgaaatc gggcgatctg gccaaggaca agaacctgtt gttgaccacg atcctggccg
     5701 acgcgatcaa cctgggcctg accaagatgg ccgagtcctg ccccggcacg acctacgcga
     5761 agctcgcttg gctgcaagcc tggcataccc gcgacgaaac gtactcgaca gcgttggctg
     5821 aactggtcaa cgctcagttt cggcatccct ttgccgggca ctggggcgat ggcaccacat
     5881 catcatcgga cggacagaat ttccgaaccg ctagcaaggc aaagagcacg gggcacatca
     5941 acccaaaata tggcagcagc ccaggacgga ctttctacac ccacatctcc gaccaatacg
     6001 cgccattcca caccaaggtg gtcaatgtcg gcctgcgcga ctcaacctac gtgctcgacg
     6061 gcctgctgta ccacgaatcc gacctgcgga tcgaggagca ctacaccgac acggcgggct
     6121 tcaccgatca cgtcttcgcc ctgatgcacc tcttgggctt ccgcttcgcg ccgcgcatcc
     6181 gcgacctggg cgacaccaag ctctacatcc cgaagggcga tgccgcctat gacgcgctca
     6241 agccgatgat cggcggcacg ctcaacatca agcacgtccg cgcccattgg gacgaaatcc
     6301 tgcggctggc cacctcgatc aagcagggca cggtgacggc ctcgctgatg ctcaggaaac
     6361 tcggcagcta cccgcgccag aacggcttgg ccgtcgcgct gcgcgagttg ggccgcatcg
     6421 agcgcacgct gttcatcctc gactggctgc aaagcgtcga gctacgccgc cgcgtgcatg
     6481 ccgggctgaa caagggcgag gcgcgcaatg cgctggcccg tgccgtgttc ttcaaccgcc
     6541 ttggtgaaat ccgtgaccgc agtttcgagc agcagcgcta ccgggccagc ggcctcaacc
     6601 tggtgacggc ggccatcgtg ctgtggaaca cggtctacct ggagcgtgcg gcgcatgcgt
     6661 tgcgcggcaa tggtcatgcc gtcgatgact cgctattgca gtacctgtcg ccactcggct
     6721 gggagcacat caacctgacc ggtgattacc tatggcgcag cagcgccaag atcggcgcgg
     6781 ggaagttcag gccgctacgg cctctgcaac cggcttagcg tgctttattt tccgttttct
     6841 gagacgaccc ctattatggc gaaggtatca ttcaggaagc gttgatgaag gattatctct
     6901 gtaaaaagtt attcaaccgg ctttccggta ccctggtgat cagagcgcgc tgcggcaata
     6961 acattactgg cctggcatgt tgcaatattc tttatccctc gccccgatac agcggtcagc
     7021 tgcatattaa agagctgtat gtttctcagt gcgatcgaaa taagggcacg ggtaaagcga
     7081 taatgcgctt tatagcccgg cttgcgcttg aacaggaatg ccttagcctt agctggaacg
     7141 ctgaaaaatc caaccccggc gctaaccgtt tttatcaggc tctgggaggc agaataaatg
     7201 atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt
     7261 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     7321 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     7381 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc
     7441 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     7501 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     7561 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     7621 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     7681 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     7741 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     7801 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     7861 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     7921 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     7981 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     8041 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     8101 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     8161 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     8221 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     8281 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     8341 cgtc
//
LOCUS       DAJTRQ010000150         8309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000150 DAJTRQ010000000
VERSION     DAJTRQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..351)
                     /locus_tag="OO332_003592"
     CDS             complement(<1..351)
                     /locus_tag="OO332_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="catalyzes the formation of AMP from
                     adenosine-3',5'-bisphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5943916.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYA"
     gene            541..2484
                     /gene="cpdB"
                     /locus_tag="OO332_003593"
     CDS             541..2484
                     /gene="cpdB"
                     /locus_tag="OO332_003593"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5943917.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(2610..2990)
                     /locus_tag="OO332_003594"
     CDS             complement(2610..2990)
                     /locus_tag="OO332_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5943918.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3079..3939
                     /gene="qorB"
                     /locus_tag="OO332_003595"
     CDS             3079..3939
                     /gene="qorB"
                     /locus_tag="OO332_003595"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5943919.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4048..5013
                     /locus_tag="OO332_003596"
     CDS             4048..5013
                     /locus_tag="OO332_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5943920.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5121..5783
                     /gene="ytfE"
                     /locus_tag="OO332_003597"
     CDS             5121..5783
                     /gene="ytfE"
                     /locus_tag="OO332_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5943921.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5828..7240)
                     /gene="cycA"
                     /locus_tag="OO332_003598"
                     /gene_synonym="dagA"
     CDS             complement(5828..7240)
                     /gene="cycA"
                     /locus_tag="OO332_003598"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5943922.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7548..8168)
                     /gene="fklB"
                     /locus_tag="OO332_003599"
     CDS             complement(7548..8168)
                     /gene="fklB"
                     /locus_tag="OO332_003599"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5943923.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2132 a         2170 c         2098 g         1909 t
ORIGIN      
        1 cgcatacacc acacctaaaa tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa
       61 ttcgccatta cgtttaataa actctttagt accatccagc gggtctacca gccagtaacg
      121 ctgccagtgc tgacggactt cccaaccggg aggatcttct tcagaaagga ccggaatatc
      181 cggtgccagc gtacgtaaac cgtccatgat aacggtgtga gcggcaatat ccgctgccgt
      241 taccggagaa ttgtccgctt tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg
      301 cataatggca tcgcctgcat tccgtgcaag ctggcatact tgatctaaca tttctccacc
      361 tcgtctctgt gagcggtgtt aacttattgt tttacttata ccctatcgtt aatgaatgcg
      421 ccaactgtga tagtgtcatc attttcaaag cgtaaaattg tggcattctt cactgttcta
      481 taagtaaggc gtttattctc ccttttcttt cgtattcccg atgataaaag gatgtccctg
      541 atgattaagt ttagcgcaac gctcctggcc acgctgattg ccgccagtgt gaatgcagcg
      601 acggtcgatc tgcgtatcat ggaaaccact gatctgcata gcaacatgat ggatttcgat
      661 tattacaaag acaccgccac ggaaaaattc ggactggtac gtacggcaag cctgattaac
      721 gatgcccgca atgaagtgaa aaacagcgta ctggtcgata acggcgattt gattcagggg
      781 agtccgctgg ccgattacat gtcggcgaaa ggattaaaag caggtgatgt tcatccggtt
      841 tataaggcgc tgaatacgct ggattatacg gtcggtacac tcggcaatca tgaatttaac
      901 tacggtctgg attacctgaa aaatgcgctg gcgggagcga aattccctta tgtaaatgcc
      961 aacgtcattg acgccagaac caaacagcca atgtttacac cgtatttaat taaagatacc
     1021 gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg
     1081 ccgccgcaaa tcatgggctg ggataaagct aatttatccg gaaaagtgac ggtgaatgat
     1141 attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcatt
     1201 gtcgttctgg cgcattccgg gctgtctgcc gatccgtata aagtaatggc ggaaaactca
     1261 gtttattacc tcagtgaaat tccgggcgtt aacgccatta tgtttggcca tgctcacgcc
     1321 gttttcccgg gtaaagattt tgctgatatc gaaggggctg atatcaccaa aggcacgctg
     1381 aatggtgttc cggcggtaat gccgggcatg tggggcgatc atcttggggt ggtcgactta
     1441 caactcagta atgacagcgg taaatggcag gtgacgcagg cgaaagcgga agcacggccg
     1501 atttacgaca tcgccaataa aaaatccctc gcggcggaag acagcaagct ggtagaaaca
     1561 ctcaaagccg atcacgatgc cacacgccag ttcgtcagca agccaatcgg taaatctgcc
     1621 gacaatatgt atagctatct ggcgctggtg caggacgatc cgaccgtgca agtcgtgaac
     1681 aacgcgcaaa aagcgtatgt cgaacattac attcagggcg atccggatct ggcaaaactg
     1741 ccggtgcttt cagctgccgc accgtttaaa gttggtggtc gcaaaaatga cccggcaagc
     1801 tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg ccgccgatct ttatctctac
     1861 cccaatacgc tgattgtagt gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc
     1921 tccgccggac agtttaacca gattgctcct aacagcacga aaccacagtc actcatcaac
     1981 tgggatggtt tccgcactta taactttgat gttattgatg gtgtgaatta tcagattgat
     2041 gttacccaac ccgcccgcta tgacggcgag tgccagatga ttaatgccaa tgcagagcgg
     2101 attaagaacc tgacctttaa tggcaaaccg attgatccga acgctatgtt cctggttgcc
     2161 accaataact atcgcgctta cggcggcaaa tttgccggta cgggcgacag ccatatcgct
     2221 tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa
     2281 cgtgcggggg aaattcatcc ggcggcagat aacaactggc gtttagcacc gatagctgcc
     2341 gataagaaac tggatatccg tttcgaaact tccccgtcag ataaagccgc agcgtttatt
     2401 aaagagaaag ggcagtatcc gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt
     2461 tatcaggtgg atttgagtaa gtaaaacact tctttttcgg cctataaatc aaccgcatcc
     2521 ggcatttatt ggcggatgcg atgctgccgt atcttatcca ccctacaagc catgaaccgt
     2581 agaccggata agcgcagcgc atccggcagt tatgccgcac gttcatcccg caccgccaac
     2641 acctcgggca aattcaactc aatccagtcc gccagtgcag caaccttttc gctcacctgc
     2701 tcgcccagcg gcgtgaggct atattccaca tgcggcggca ccaccggata cgcgatacgg
     2761 ttaagaaaac catcctgttc taacgcctgt aacgactgcg caagcatctt ttcactcacc
     2821 ccaccgattt tgcgccgcag gtcgctaaag cgatgagtac cttcgcgtag cgccaccaga
     2881 atcaacaccc cccaacggct ggtgacgtgt ttcaacacct cgcgcgacgg gcactgttcc
     2941 gcaaagagat taccctcttt cagttgttgt gacaggctaa cctgactcat ttcatactta
     3001 cctttttgca cgtacttact aaaagtaagt ttaggtgtta gcgtatttaa acacaagaca
     3061 aaacgatgga gacttcccat gatcgctatt actggtgcca ctggccaact tggtcactat
     3121 attattgaat ccttgatgaa aacggttcct gccagccaaa tagtggctat cgttcgtaat
     3181 ccggcaaaag cccaggccct ggcagcacaa ggcattaccg tgcgtcaggc tgactacagc
     3241 gatgaagccg cactgacatc tgcacttcag ggagtggaaa aactactgct gatctcttcc
     3301 agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta ttaatgccgc aaaggcggct
     3361 ggcgtgaaat ttatcgctta taccagcctg ctacatgcag atacctcccc gctcggcctc
     3421 gccgatgagc acatcgagac ggagaaaatg ctggctgatt ctggcatcgt ttacaccctg
     3481 ctgcgcaacg gctggtacag cgaaaactac ctcgccagcg ccccggcagc actggaacac
     3541 ggcgtattta tcggtgcggc gggcgatggc aaaatcgcct cagcaacgcg ggcagattat
     3601 gcgacagctg cggcactcgt gattagcgaa gccggtcacg aaggcaaggt ttacgaactg
     3661 gcgggcgata gtgcctggac gttgacacag ttagcggcag agctgaccaa acagagcggc
     3721 aaacaggtta cctatcaaaa tctgagcgaa gccgatttcg ccgcggcact gaaaagcgtc
     3781 ggactgcccg acggactggc ggatatgctg gcggattctg acgttggcgc atcgaaaggt
     3841 ggtctgtttg ataacagcaa aacgcttagc aaattgattg gccgcccaac gacaacgtta
     3901 gccgaaagcg taagccatct ttttaatgtt aataactagt taattaaagt ggcatcctcc
     3961 cgcatcctct ctgataatga cgggatgccg ggagcaatca tgtctgcttc ctgaacgttt
     4021 cttctgacag accaatggat gccagtaatg attagcggcg tgctgtacgc cctgttagca
     4081 gggttgatgt gggggcttat ttttgtcggg ccgttgatcg tgccggaata cccggcgatg
     4141 ttacagtcga tggggcgtta tctggcgtta gggttaattg cgctacccat tgcctggctg
     4201 ggacgcgtgc gtctgcgtca gttggcgcgt cgggactggc ttaccgcctt gatgctcact
     4261 atgatgggca acctcattta ttacttctgc cttgccagtg ccattcaacg tactggcgcg
     4321 cctgtttcca cgatgattat cggcaccctg ccggtggtga ttcctgtctt tgccaatctg
     4381 ctttatagcc agcgcgacgg caaactcgcg tggggaaaac tcgccccggc actgatttgt
     4441 attggcatcg gcctggcgag tgtgaatatt gctgagttaa accacggact ccccgatttt
     4501 gactgggcac gttatacctc tggcatcgtg ctagcgttag tttccgtggt ctgctgggca
     4561 tggtatgccc tgcgcaacgc ccgctggctg cgggaaaatc ccgacaaaca tccgatgatg
     4621 tgggcgacgg cgcaggcgct ggtcacgctg ccggtttctc tcatcggcta tctcgtcgcc
     4681 tgttactggc tgaatataca aacgccggac ttctccttac cttttggccc ccgtccgctg
     4741 gtgtttatta gtctgatggt tgcgatagcc gtgctttgct catgggttgg cgcactctgc
     4801 tggaacgtcg ccagccagcg attaccgaca gtgattctcg ggccgctgat tgttttcgaa
     4861 acgctggcag gtttgctgta caccttttta ctccgccagc aaatgccgcc gctaatgacg
     4921 ctgagcggta tcgcgctgtt agtgattggc gtggtcatcg cggtcagagc aaaaccagaa
     4981 aaacctttaa ctgaatctgt ctcagaaagt tgacatgctg gcagtgagtt aaataagcct
     5041 ctgctacgta agggttatag cttttgcctt aaaggtgtat ttaaaataca tcttatctta
     5101 ttaagaatga ggtatcagct atggcttatc gcgaccaacc tttaggtgaa ctggcgctct
     5161 ctatccctcg cgcttcggct ctgtttcgta aatatgatat ggattactgc tgtggcggta
     5221 agcagacgct ggcgcgcgcg gcggcacgta aagaactgga tgttgaggtc attgaagctg
     5281 aactggcaaa gcttgctgaa caaccgattg agaaagactg gcgtagcgcc ccgctggcag
     5341 aaatcattga ccatatcatc gtgcgctacc acgatcgtca ccgcgagcag ctgccggagc
     5401 tgatcctgca agcgactaaa gtcgagcgcg ttcacgccga caaaccgagc gtgccaaaag
     5461 ggctgacaaa atacctgacc atgctgcatg aagagctttc cagccacatg atgaaagaag
     5521 agcagatcct cttcccgatg atcaaacaag gcatgggcag ccaggcaatg gggccaatca
     5581 gcgtaatgga aagcgagcac gatgaagcgg gcgaactgct agaagtgatc aaacacacta
     5641 ccaataacgt cacaccgccg ccagaagcct gcaccacctg gaaagcgatg tataacggca
     5701 ttaatgaact gattgatgac ctgatggatc acatcagtct ggaaaacaat gtactgttcc
     5761 cacgcgccct agcgggtgag tgatctaaaa gctggatggc attgcgccat ccagcatgat
     5821 aatgcggtta tttccgcagt tcagcagccc gcttcttacc aataaacagc cagcccaacc
     5881 ccagcgcgat aaaccacagc ggggtaacca gcagcgcctg gcgagtgtca tcttccagtg
     5941 tcagcaacac gaccacgaac acaaagaacg ccatacatac ccagcacatc agcttgccga
     6001 gcggcatctt gtagatcgac ttttcatgca gatgaggacg ctgtttgcgg tacaccaggt
     6061 acgagcaaag gataatcgtc cagacgaaca taaacagaat cgcggaaacg gttgtaatca
     6121 tcgtgaacgc gccaatcacg ctaggattca catacaacat caccacgccg ccgagcagac
     6181 agatacacga gaaggtcaac cctttcgcgg gtactgcgcg cttagaaagt ttagcgaacg
     6241 ctttcggcgc aacaccttcc tgcgccagac caaacagcat acggctggta gagaagacac
     6301 cgctgttagc ggaagacgcc gcagaggtca gcaccacaaa gttgatcacg ctggcagccg
     6361 caggcaaacc taccaacaca aacagctcaa cgaacgggct tttctccggg actaccgaac
     6421 tccacggtgt cacggacata atcacaatca gcgcgaagac gtagaacata atgatacgga
     6481 tcggaatgga gttaatcgcg cgtggcagtg atttctccgg atctttggtt tccgcagctg
     6541 ttgtacctac cagctcaatc cccacgaaag cgaaaaccgc tatctggaat ccggcaaaga
     6601 agccacttaa acctttcggg aaccagccgc cgtcattcca caaatgcgcg aatgacgctt
     6661 ccacaccggt cggtgactga aagtgcatcg ccaccatgac caggccgacg acaatcagcg
     6721 acacgatggc gacgattttg atcatcgcaa accagaactc catctcaccg aacattttca
     6781 cggtggcaag gttgagcgtc agcagcagca ctatcaccgc cagcgaggcg acccagtcgg
     6841 agaaatcggg gaaccagaac tgagcataag ccgtgatcgc caccacgtct gccataccgg
     6901 ttacaaccca gcagaaccag taagtccagc cggtgaaata tcccgcccac ggccccagta
     6961 aatcggaagc gaagtcacta aaagatttgt attccagatt cgaaagcagc aattccccca
     7021 ttgcccgcat cacgaaaaag agcataaaac caatgatcat ataaacgaaa atgatcgacg
     7081 gcccggcaag gctaatcgtt ttgccggacc ccataaacaa cccagtacca atggcaccgc
     7141 caatggcaat aagctgaata tgtcggtttg tgagattgcg ccgtagcgac tgttcagccg
     7201 gagcctgatc atcggcaacg acttttacct gatctaccat gttttttctt cctgtacctg
     7261 tctgtgttgt tcaggctcta cggcctttag tcagtctatg atacgacgaa tctgtatcgg
     7321 gactcatcga tattaggtaa caatacgcgg gatgaatact aagatttaga tctaatgtta
     7381 attttatgtt taaagtgagt gtcatatcac ggtgataacg cgaaacagct cacaaaaata
     7441 cacgcttacg gggtctttgc aacacaaaat agcgccgttt ggcaaactga agggtttatt
     7501 gctgaatgcc tgctcccctc tggttcgagg ggaacagaat gcgctgctta gaggatttcc
     7561 agcagttcga cttcaaacac cagggtgctg aacggaggga tggatgcacc tgcgccgcgc
     7621 tcgccatatg ccagttcctg cgggatagtc agttcccatt tagaacctac cggcatcaga
     7681 gtcagtgctt caatccagcc agggatcacg ccattaaccg ggaattcagc gggttcacca
     7741 cgagcaacgg agctgtcaaa cacggtgccg tcgatcagtt taccggtgta atgaacacga
     7801 acgcggtcgg tacgtgccgg aattgcgcct tcaccttggt tgatcacgcg gaattgcagg
     7861 ccagattcgg tgctattcac accttctttt ttggcgtttt cttccaggta tttcacacct
     7921 tcagcagcca tcgcctggaa acgctgacga cgaacggcat cggcgcgttc gtggatttca
     7981 cgcagcgcgc gatgcaccac atcaaccggc acagccggat gtttgccttc cagcgcatcg
     8041 gcaatacctg caatcagtgc ttctggcagc agcccttcca ggccagattc actcagttgt
     8101 tgccctacct gcaaaccaat gccgtagctt gcttgcgctt cgatggtgtc aaaagttggg
     8161 gtggtcatcg tatttccttt cttagggtat aaaagtagcg ggcagcatat cagccacact
     8221 tctttgggta aaactttgtc gcaggaatga tgacaaatcc cggggaaaaa gaaacaatag
     8281 caatatcccg tatgaatata ataaaggcg
//
LOCUS       DAJTRQ010000151         8243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000151 DAJTRQ010000000
VERSION     DAJTRQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(60..458)
                     /gene="vapC"
                     /locus_tag="OO332_003600"
     CDS             complement(60..458)
                     /gene="vapC"
                     /locus_tag="OO332_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5943924.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(458..685)
                     /gene="vapB"
                     /locus_tag="OO332_003601"
     CDS             complement(458..685)
                     /gene="vapB"
                     /locus_tag="OO332_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5943925.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            767..1186
                     /locus_tag="OO332_003602"
     CDS             767..1186
                     /locus_tag="OO332_003602"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5943926.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1173..6038
                     /gene="traI"
                     /locus_tag="OO332_003603"
     CDS             1173..6038
                     /gene="traI"
                     /locus_tag="OO332_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR5943927.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6058..6804
                     /gene="traX"
                     /locus_tag="OO332_003604"
     CDS             6058..6804
                     /gene="traX"
                     /locus_tag="OO332_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5943928.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6859..7419
                     /locus_tag="OO332_003605"
     CDS             6859..7419
                     /locus_tag="OO332_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5943929.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7550..7762
                     /locus_tag="OO332_003606"
     CDS             7550..7762
                     /locus_tag="OO332_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5943930.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8006..>8243
                     /locus_tag="OO332_003607"
     CDS             8006..>8243
                     /locus_tag="OO332_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5943931.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1887 a         2073 c         2637 g         1646 t
ORIGIN      
        1 aatgtgcacc gggaacgcgg ggaggatgtt gagccgggag atgatttctg atgaacaggt
       61 cagctccagt cttcagttct caggccgccc acacgttcaa attcccgggt gttattagtc
      121 acaataatca gcccccgact gcgggcatga cctgcgatca tttgatcaaa tggcccgaca
      181 gggcgtccct gacgggcaag ttctgctctt atctggccgg tgtgtgtggc tgcagcagcg
      241 tcgtaatcca gaacatcaat gcgggaaaca aatccctcga tcacagcgag attacgttca
      301 ggcatctggc ttttttctgc accatatatc agctccatca gggtgaccga actgatacac
      361 attttcccct ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt ctttatcgta
      421 aaaatgcaga tgttggtatc gagcataaac ttcagcatca gaatgactcc ctttcctgca
      481 tgccgggctg ttccctgtta tccataaaat cggtgctgac gctgtgtccg tcgaaccatt
      541 cgtcccatgt ctctccggct ggcgtaatga ttctggttcg tccgacagca atcacttcaa
      601 cgcgttttac gttttccggc aatgcaaccg cttttggcag tctgaccgcc tggctgcggt
      661 tgctgagaaa tacggtggtt tccattattt gcctccttat gtggatatgt cttgtgtata
      721 tctattgtgg gattgatacc gggatatgtc aaagggatat acgtttatga tgagtatcgc
      781 gcaggtcaga tcggccggaa gtgccggtaa ttattatacc gacaaggata attactatgt
      841 gctgggcagc atgggagaac gctgggccgg ccagggggct gaacaactgg ggctgcaggg
      901 cagtgtcgat aaggatgtct ttacccgcct tctggagggc aggctgccgg acggggcgga
      961 tttaagccgc atgcaggatg gcagtaacag gcatcgtccc ggctacgacc tgaccttctc
     1021 cgctcccaaa agtatctcca tgatggccat gctcgggggg gacaaacgtc ttattgaagc
     1081 acataaccag gccgtggatt ttgctgtccg tcatcacccg tgtggaggca ctggcctcca
     1141 cacgggtgat gacggacgga cagtcagaaa cggtgctgac cggtaatctg gtgatggcac
     1201 tgtttaacca cgacaccagt cgcgatcagg aaccacagtt acacacgcat gcggtggtga
     1261 ctaatgttac gcagtataac ggcgaatgga agacactgag cagtgacaaa gtggggaaaa
     1321 cggggttcat tgagaatgtg tacgctaatc agattgcctt tggcaggctc taccgggaaa
     1381 aactgaaaga gcaggttgag gcgctgggct atgaaactga agtggtcggt aagcacggta
     1441 tgtgggaaat gccgggtgta ccggtggagg ccttttccgg acgctcacag actatccggg
     1501 aggccgtcgg ggaagacgcc tcgctgaaat cccgggatgt ggcggcgctg gatacgcgta
     1561 aatccaaaca gcacgtcgat cctgaagtca gaatggccga gtggatgcag acgctgaagg
     1621 aaaccgggtt cgacatccgg gcatatcgtg acgcagcgga acaacgggca tatacccgca
     1681 cgcagacacc cggacccgct tcacaggacg ggccggatgt gcagcaggcg gtgacacagg
     1741 ccattgccgg attaagtgaa cgcaaagtgc agtttatgta tacggacctg ctggccagga
     1801 cggtcggcat actgccgccg gaaaatggtg tgattgaacg ggcacgcgcc ggtatcgatg
     1861 aggccatcag cagtgagcag cttatccccc tcgatcgtga gaaggggctg ttcacgtccg
     1921 ggattcatat gctcgatgag ctgtcagtcc gggcactcag tcgtgacatc atgaaacaga
     1981 accgggtgac cgtacatccg gagaaaagtg tcccccggac ggccggttac agcgatgccg
     2041 tcagtgttct ggcacaggac cgtccgtcgc tggccattgt gtccgggcag ggcggtgcag
     2101 ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc ccgggagcag gggcgggagg
     2161 tgcagattat cgctgctgac cgtcgctcgc agatgaacct gaagcaggat gaacggctgt
     2221 ccggtgaact gataaccgga cgtcgtcagt tgctggaagg catggccttc acgccgggca
     2281 gtaccgttat cgtcgaccag ggcgaaaaac tctccctgaa agagacgtta accctgctgg
     2341 atggtgccgc acgtcataac gtacaggtcc tgataaccga cagcgggcag cgaaccggta
     2401 caggcagtgc actgatggcc atgaaggatg ccggggtgaa cacatatcgc tggcaggggg
     2461 gagaacagcg accggccacc atcatcagtg aaccggaccg taatgtccgc tatgcccggc
     2521 tggccggaga ttttgcggcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg
     2581 gagtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acacagggcg
     2641 tgctcggaca cccggaggta accatgaccg ccctgtcacc ggtctggctg gacagccgga
     2701 gccgttatct gcgggatatg taccgcccgg gaatggtgat ggagcagtgg aacccggaga
     2761 cacgcagtca tgaccgctat gtgacagaac gtgtgacggc gcagagtcac agcctgaccc
     2821 tgcggaatgc gcaggtcgaa acgcaagtgg tgcgtatttc ttccctggac agcagctggt
     2881 cgctgttccg gccggaaaaa atgccggtgg cagacggtga gcgtctgagg gtgacaggga
     2941 aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
     3001 atgcgatgac ggttgttgtg ccgggacggg ctgaaccggc caccctgcct gtgagcgatt
     3061 caccgttcac ggcactgaag ctggagaacg gctgggtgga aacgcccggg cattccgtca
     3121 gcgacagtgc gacggttttt gcctccgtca cacagatggc aatggacaat gccaccctga
     3181 atggtctggc ccgcagtggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
     3241 ctgcggaaaa acttgcccgc catccctcct ttacggtggt ttctgagcag ataaaggcgc
     3301 gtgccggtga gacattgctg gaaaccgcta tcagtctgca gaaagccggg cttcacacgc
     3361 cggcacagca ggccattcat ctggcccttc ctgtgctgga aagtaaaaac ctggccttca
     3421 gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc ggttttgctg
     3481 acctgggagg ggaaatcaat gcgcagataa aacggggtga tttactgtat gtggatgtgg
     3541 caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca
     3601 ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc gctgatggag agagtacctg
     3661 gcgaactcat ggagaaactg acatcaggac agcgcgccgc cacccgtatg atactggaaa
     3721 cgtctgaccg tttcacggtg gtgcagggct atgccggtgt gggtaagacc acacagttcc
     3781 gggcggtgat gtcagccgtg aacatgctgc cggagagtga acgtccccgt gtcgtggggc
     3841 tggggcccac acaccgtgcg gtcggggaga tgcgcagcgc cggcgtggat gcacagacac
     3901 tggcgtcctt tctgcatgac acgcagcagc gcagcggaga aacgccggat ttcagcaaca
     3961 cgctgttcct tctcgatgag agctctatgg tgggcaatac cgacatggca cgggcatacg
     4021 ccctgattgc ggccggtggc ggtcgggctg tggccagcgg tgacacggac cagctgcagg
     4081 ccatcgcgcc cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggtca
     4141 tcatgaagga gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta
     4201 accgggatgt ggaaagggcg ctgtccgggc ttgagcgtgt gaaaccgtct caggtgccac
     4261 gtctggaggg cgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag
     4321 cgaaactggc agaagcgcag cagaaggcga tgctgaaagg cgaggctttt ccggatgtcc
     4381 ccatgacact gtatgaagcc attgtccgcg actataccgg caggacaccg gaagcacggg
     4441 agcagacgct gattgtcacg cacctgaatg aggaccggcg cgtactgaac agcatgattc
     4501 atgatgcacg ggaaaaggcc ggagaactgg ggaaggtgca ggtcatggtg cctgtcctga
     4561 acacagcgaa tatacgcgac ggggagctgc gtcgtctctc cacctgggag aataacccgg
     4621 atgcccttgc cctggtggat aatgtgtatc accggattgc cggtatcagc aaggatgacg
     4681 ggctgataac cctgcaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg
     4741 tggctgaagg cgtcacgctg tacaccccgg acaccatccg ggtggggacc ggtgacagga
     4801 tacgcttcac gaagagtgac cgggagcgcg gttatgtggc caacagcgtc tggacggtga
     4861 cagcggtttc cggtgacagt gtcacacttt ctgacggtca gcagacccgt gtgattcgcc
     4921 ccggtcagga gcgggcagag caacatattg acctggccta tgccatcacc gcccacggtg
     4981 cacagggggc gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac
     5041 tgatggccgg ctttgagtca gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg
     5101 cgtacaccga taaccgtcag ggctggacgg atgccatcaa caatgccgta cagaaaggaa
     5161 ctgcccacga tgtgtttgag ccgaaaccgg accgggaggt catgaatgca gagcggctgt
     5221 tcagtacggc gcgggagctg cgggacgtgg cggcaggccg tgccgttctc cgtcaggcgg
     5281 ggctggccgg gggagacagt cctacacggt ttattgctcc gggacgtaaa tatccgcaac
     5341 cgtatgtggc actgccggcg tttgaccgta acggcaagtc cgccggtatc tggctgaacc
     5401 cgctgaccac ggatgacgga aacgggctgc ggggattcag cggtgaaggt cgggtaaaag
     5461 gcagcgggga tgcgcagttc gtggccctgc agggcagccg taacggagaa agcctgctgg
     5521 ctgataatat gcaggatggc gtccggattg cccgtgataa tcctgacagt ggtgtggtgg
     5581 taagaatcgc cggtgaaggc cgtccgtgga atcccggtgc catcaccggt ggccgcgtgt
     5641 ggggggatat cccggacaac agcgtccagc cgggagccgg aaatggcgaa ccggtcacgg
     5701 cagaggtact ggcacagcgg caggctgaag aggccatccg ccgtgaaacg gaacgtcgcg
     5761 cagatgaaat tgtccgtaaa atggcagaga acaaacctga cctgccggac ggcaaaacag
     5821 agcaggctgt cagggaaatt gccggtcagg agcgtgaccg ggctgccata actgaacggg
     5881 aagccgcgct gccggagagt gtactgcgtg aaccacaacg ggtgcgggaa gcggtccggg
     5941 aagttgcccg ggaaaatttg ctgcaggagc gtctgcagca gatggagcgg gatatggttc
     6001 gtgacctgca gaaagagaaa accccgggcg gagactgata caggaagaca aactctgatg
     6061 acaacggata acacgaacac gacacgtaac gattcactgg ctgcacggac cgatacctgg
     6121 ttgcagtcat ttctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg
     6181 gtgctgatgg tactggacca catcaatctg atattccagc tgaagcagga atggatgttt
     6241 ctggccgggc gaggagcctt tccgcttttt gccctggtgt gggggctgaa tctgtcccgt
     6301 catgcgcata tccggcaacc ggccattaac cggctgtggg gatggggaat tattgcccag
     6361 ttcgcatatt atctggcagg ctttccttgg tatgagggga atatcctgtt tgcctttgca
     6421 gtggcggcac aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc
     6481 atactcctga tggccctgtg ggggcctttg tccggcacca gttacggtat tgccgggttg
     6541 ctgatgctgg cagtcagcaa ccggctgtac cgggcggaag acagagcgga acgtctggcg
     6601 ctggtcgcct gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgcagcg
     6661 gcggtggccg gtcttgtgat gacggtgctg accgttggtc tggtgttgtg tgccggaaaa
     6721 tcattacccc gtttctggcc cggagacttt ttcccgacgt tctatgcctg tcatctggct
     6781 gtgctgggcg ttctggcgct gtgacgggtg tggtatcttt ggccgcaaga ggatgattcg
     6841 tcagaggcag aacacagcat gatagagcag aagcgaccgg tactgacact gaagcggaag
     6901 acagagggaa cagcgcctgt ccgcagccgg aaaaccatca tcaatgtcac cacgccacca
     6961 aagtggaagg tgaaaaagca gaagctggcc gagaaagcgg cccgtgaagc agagctggcg
     7021 gcaaaaaaag cgcaggccag acaggcgctg tccatttatc tgaacctgcc cacgctggat
     7081 gaggctgtga ataccctgaa gccctggtgg ccgggattat ttgacggtga cacgccctgg
     7141 cttctggcct gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg
     7201 ctctcgcata aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg
     7261 tgtgccatga aagccggtgc ctgccggtat gacacggaag ggtatgtgac ggagcatatt
     7321 tctcaggagg aggaagcgta tgcgggagcg cgtctggcga aaattcgtcg ccagaaccgg
     7381 ataaaggcag aacttcaggc cgtgcttgat gagaaataaa aaaagcctcc cccagagaga
     7441 ggaggcaggg aaataaggct taaaaggaaa taacttccgc ttccgaaagg aagccagtcc
     7501 actgaattat ttatcacaat aatactgaca actaaagggg gacaagaaaa tgaatggatt
     7561 cagaaacagt tcacggaacg gccaggtctg gcgttaccag cgtgccggcg ggcgggccgt
     7621 tattctggaa gtcagtggac gctggatgga agcggcagaa gcatggcgac gggctgcctg
     7681 tgttgcgcct aggacagact ggcagcagtt tgcccggaaa agggctgaac actgccaccg
     7741 gcgctgccgg ggaagggtgt aacgaaaaaa gcagccttca ttactctagg agtcagaagg
     7801 ctgaaaggag tttatgagat ttgcttctta atatccggtc gggcagtatg accggacagt
     7861 tctgagtagc acagggtctt ttccgcatta agtcgatttt ttatattttg tgattaattt
     7921 cacaaaataa ggtgttattc aatgtgtgct gcactattca ggggggctga aatacaggtg
     7981 gcatttatct gatatggaga ataacatgag gaaatatatt ccactggtat tatttatctt
     8041 ttcatggccg gtattgtctg cagatattca tggacgggtt gttcgtgtac tggacggtga
     8101 taccatcgag gtcatggact cactgaaggc ggtgcggatc aggctggtaa atattgatgc
     8161 accggaaaag aaacaggatt atggccggtg gtcaacagac atgatgaaat cactggtcgc
     8221 aggaaaaacg gttaccgtca cat
//
LOCUS       DAJTRQ010000152         8200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000152 DAJTRQ010000000
VERSION     DAJTRQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            59..460
                     /locus_tag="OO332_003608"
     CDS             59..460
                     /locus_tag="OO332_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5943932.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="OO332_003609"
     CDS             570..1901
                     /locus_tag="OO332_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5943933.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /gene="narU"
                     /locus_tag="OO332_003610"
                     /pseudo
     CDS             2225..3613
                     /gene="narU"
                     /locus_tag="OO332_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3695..7328
                     /gene="narZ"
                     /locus_tag="OO332_003611"
                     /pseudo
     CDS             3695..7328
                     /gene="narZ"
                     /locus_tag="OO332_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7325..>8200
                     /gene="narH"
                     /locus_tag="OO332_003612"
     CDS             7325..>8200
                     /gene="narH"
                     /locus_tag="OO332_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5943934.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2191 a         2029 c         1911 g         2069 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 actcggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc
//
LOCUS       DAJTRQ010000153         8148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000153 DAJTRQ010000000
VERSION     DAJTRQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(52..591)
                     /locus_tag="OO332_003613"
     CDS             complement(52..591)
                     /locus_tag="OO332_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5943935.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(656..1204)
                     /gene="fimA"
                     /locus_tag="OO332_003614"
     CDS             complement(656..1204)
                     /gene="fimA"
                     /locus_tag="OO332_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5943936.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1685..2281)
                     /gene="fimE"
                     /locus_tag="OO332_003615"
     CDS             complement(1685..2281)
                     /gene="fimE"
                     /locus_tag="OO332_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5943937.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2759..3360)
                     /gene="fimB"
                     /locus_tag="OO332_003616"
                     /pseudo
     CDS             complement(2759..3360)
                     /gene="fimB"
                     /locus_tag="OO332_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4814..5530
                     /gene="nanC"
                     /locus_tag="OO332_003617"
     CDS             4814..5530
                     /gene="nanC"
                     /locus_tag="OO332_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5943938.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5550..6656
                     /gene="nanM"
                     /locus_tag="OO332_003618"
     CDS             5550..6656
                     /gene="nanM"
                     /locus_tag="OO332_003618"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5943939.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6721..7701
                     /locus_tag="OO332_003619"
     CDS             6721..7701
                     /locus_tag="OO332_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5943940.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2418 a         1741 c         1699 g         2290 t
ORIGIN      
        1 gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata
       61 ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc
      121 acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc
      181 tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt
      241 ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac
      301 accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat
      361 aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg
      421 ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat
      481 gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag
      541 agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt
      601 tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt
      661 gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg
      721 cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac
      781 taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtccaggatc tgcacgccaa
      841 cgtttgttgc actacctgca gcagaactct gcagagctag tacatcagta cgcgtagcat
      901 caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat
      961 tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg
     1021 tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg
     1081 cgttaacaac ttccccttta aagttaatgg tcccaccatt taccgtcgta gtatcggcca
     1141 gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt
     1201 tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca
     1261 ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa
     1321 catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt
     1381 tgttggctta atattctatt gttatcttta tttatagatt tttatattgc atgaggtggt
     1441 ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag
     1501 cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc
     1561 tttttgtcgt tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat
     1621 ggacagtttg gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt
     1681 taaatcaaac ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc
     1741 cggcaaaacg agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc
     1801 cgagataatc ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag
     1861 catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc
     1921 gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag
     1981 cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt
     2041 cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat
     2101 ttattcgacc ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac
     2161 gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt
     2221 aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca
     2281 caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg
     2341 gtcaaaatta gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc
     2401 aattacatta aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat
     2461 tgatttgatt caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata
     2521 aagaaaaaag tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt
     2581 ttatttattt tgaagaacat aaaccattag attttgtatg ggtatggtta acttaatgtt
     2641 tattatgata attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt
     2701 ttgatatatc gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct
     2761 ataaaacagc atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg
     2821 cattgctggc ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga
     2881 taagtcgcgt atctattcct atattcgcca aagcaaaacc acacgaatag cgtaacatgt
     2941 gcggatgaat ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa
     3001 actgttgccg agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat
     3061 gcggataaga agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata
     3121 gcgggtgcgt tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa
     3181 gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac
     3241 aaagcaaaat cagacaataa ttacgtgttg catgaggccg gtatttgctg ctttaaggag
     3301 tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct tattcctcat
     3361 cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat aatccaacat
     3421 ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg taagtggacc
     3481 agttcttctg agcgaactta aatggagtag cgatagttac ttataataac tttgccatac
     3541 aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga tacgaatcta
     3601 ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga tatgttctgt
     3661 aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa ctagccgtct
     3721 caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca tggaacgcag
     3781 taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta ttcctgcctg
     3841 gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga tatgggggaa
     3901 cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc actacgactt
     3961 aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta agtgaaacat
     4021 catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag tgaccagtta
     4081 caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct gatggcgagt
     4141 aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt gtttagattc
     4201 taaataaatt ttttataaaa catatagtta aaagaaaatc tttagttttt gaagaccacc
     4261 gcaagtgttc gtctggcttc atatggcatc ttcttcttag aaaaagatcg acatattttg
     4321 tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga tctggtataa
     4381 caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat ctatggcaaa
     4441 catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga aaggaatatg
     4501 aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt gagtaagtga
     4561 tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac tcaggtattt
     4621 aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg agaccttata
     4681 caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg caaaaaaata
     4741 ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa
     4801 atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc
     4861 gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc gtagtggaag
     4921 ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag
     4981 catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg ccgcactcaa
     5041 tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga cggtgcgccc
     5101 gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc
     5161 ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta
     5221 ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata
     5281 tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa
     5341 acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg cctttgttct
     5401 acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc ttgaccgcca
     5461 gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt
     5521 taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg cttgctatca
     5581 tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg ccatttaaaa
     5641 gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc gcaggtacag
     5701 catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta gctgcattcc
     5761 ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg tatgtgtttg
     5821 gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta cacaaataca
     5881 accccaaaac caatcgctgg gttaaattga tgtcgcacgc gccgatgggc atggcgggtc
     5941 atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac cagaatatct
     6001 tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct atagataaaa
     6061 tcaacgccca ctattttgac aaaaaagaag aatattattt cttcaataag tttctgttgt
     6121 cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg tacggaacgg
     6181 ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc gaagccaaac
     6241 caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat ttaaaatgga
     6301 ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg gggataagca
     6361 atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa aattaccaga
     6421 acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat attcatcttt
     6481 ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc tacggagtat
     6541 cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc ggcaaagcgg
     6601 tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa aactaacgct
     6661 tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg cgaaaattat
     6721 atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg tcagtctaat
     6781 gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc tcattccaga
     6841 attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg tcactttaac
     6901 gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat
     6961 cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca
     7021 cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc gtgttgccgt
     7081 ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca cggagccagc
     7141 catgatgctt gtcgttgggg aacggatact ccgctgtacc aggatttagt cagcagaacg
     7201 cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg gatgcaaggc
     7261 gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa tcatatggtt
     7321 gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat tactgacgcg
     7381 ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca ttcgtatgaa
     7441 gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag
     7501 caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga tttaagcacg
     7561 ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact gcgaagcagt
     7621 catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga agcaattttg
     7681 cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct tgttcgttct
     7741 cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt gcgggctcga
     7801 acagcaggca attgccaata taataatctg gcacaagggt gaagaacgcg caccgcataa
     7861 accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg ccttttcgat
     7921 tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg gccacagcgt
     7981 tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt ctggcaaata
     8041 gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat attattgctt
     8101 gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcat
//
LOCUS       DAJTRQ010000154         8144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000154 DAJTRQ010000000
VERSION     DAJTRQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..895)
                     /locus_tag="OO332_003620"
     CDS             complement(<1..895)
                     /locus_tag="OO332_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5943941.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            955..1233
                     /locus_tag="OO332_003621"
     CDS             955..1233
                     /locus_tag="OO332_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943942.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1234..1761)
                     /gene="gadE"
                     /locus_tag="OO332_003622"
     CDS             complement(1234..1761)
                     /gene="gadE"
                     /locus_tag="OO332_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5943943.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2560..3132)
                     /gene="hdeD"
                     /locus_tag="OO332_003623"
     CDS             complement(2560..3132)
                     /gene="hdeD"
                     /locus_tag="OO332_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5943944.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3387..3719
                     /gene="hdeA"
                     /locus_tag="OO332_003624"
     CDS             3387..3719
                     /gene="hdeA"
                     /locus_tag="OO332_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5943945.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3835..4161
                     /gene="hdeB"
                     /locus_tag="OO332_003625"
     CDS             3835..4161
                     /gene="hdeB"
                     /locus_tag="OO332_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5943946.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4225..4871
                     /locus_tag="OO332_003626"
                     /pseudo
     CDS             4225..4871
                     /locus_tag="OO332_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4913..5443)
                     /locus_tag="OO332_003627"
     CDS             complement(4913..5443)
                     /locus_tag="OO332_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5943947.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5599..6165)
                     /gene="slp"
                     /locus_tag="OO332_003628"
     CDS             complement(5599..6165)
                     /gene="slp"
                     /locus_tag="OO332_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5943948.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6412..7635)
                     /locus_tag="OO332_003629"
     CDS             complement(6412..7635)
                     /locus_tag="OO332_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943949.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2366 a         1575 c         1705 g         2498 t
ORIGIN      
        1 ggcggctacc ttcatccact aatgccgtga catacatgcc aggcagcaag tcaccatttg
       61 ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt ttcatcaacc gtcgggtcgg
      121 agaatttcag cgtgccggtc tggctgtaac gtttaccatt ttccagattg agctgtactg
      181 gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac ctcttctttc atgcgtaaga
      241 aatcttgcac cgactgcgtg agatcgacat aaatcgggtc cagacgttgt acggtaacca
      301 gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt caccgacgat ttcccgctga
      361 cgcccgtaat cggcgaggtg acattcgcgt attgcagatt gatcgtcgcc tgttcaacag
      421 ccgctttggc gacggtgaca ttggcttctg cttcattcag ctgggtgcgc gcggtgtcgt
      481 aatcctgacg gctaacgtag ttggtcttca gcaacgatgc ctggcggtta aaggtgatgc
      541 gggcattgct ggcggtagag agcgctttcg ccagcgagcc tttggcggag tttagctcgg
      601 cctgtaaagg tgcaggatca atctgataca gcgaatcgct ctggttcact ttatcgcctt
      661 cgataaagtt gcgtttaatg ataataccgc ccacctgggg acgtatctcg gcagcttcat
      721 aaggaacggt tctaccgggc aattcgctca acacattgac cgaactaggg gagagtgtga
      781 cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc cgatttgtca tcgcaggcgg
      841 tgagcatcgc gccgcagaat aacaacggta ttaacagctt tcttcttctg ttcattttag
      901 tccctgaaaa ttcttgagaa ataacggttc tttaaattgc gcgcgaggag agatatgagg
      961 gatttacaga ccagcggcat agtggggttg tcagccagta aggtgggata caggcacagt
     1021 gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg ttctgccaac agttcctgcc
     1081 agcattcggg caacgaaggg ttctactggt ggatacacat accaggggaa tcaagtcgtt
     1141 ttagcctctc ctttagtaat caccgggggt aatgaaatca gcatctgcat ccctcgtcat
     1201 gccagccatc aatttcagtt gcttatgtcc tgactaaaaa taagatgtga tacccagggt
     1261 gacgatgtcg ctcatacgtc ttagtttcag cttgtacata atgttgaatt tctgaatctt
     1321 taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg ctttcatttt tatttttcag
     1381 aatcagttgc ataattttga actgattatg tgaaaaacat aattgtgaat tcttatgggg
     1441 caagtgttta ccataagtaa tctcaagaag atttttgata ctttctttgc ggctaacaaa
     1501 ataaataggc gctttagctt ttagtttact gatgtgataa ggtgccaaaa caataattct
     1561 ctcggcatct aatttctcca gaaatttaat cgcttcttca tcaaggatat gattgtgata
     1621 ggtatcaatg ataaccttga agggtttatt gcctactttt ttgatctctg acagggaatt
     1681 gatttttatc atttcgtgat tatctttcaa ctgccaaaag ccctgtaaaa gaaaagaatc
     1741 tttcgtcatg agaaaaatca taacttgctc cttagccgtt attgtttcga acatgtcatc
     1801 cttttcatta tttacatcct tgtccgaatc gttgttcaat atagtaaacg cctataagaa
     1861 ctttgccctt cgtgaactaa tacgtctatt aacactaatc aatacttcag atttatccat
     1921 cacgcactcc atatattgat cctaagtaag attcctggtt gttatcagct tgtaaatagt
     1981 aaacaaaaag ttaacgtttg cggattactg cccaagaata agttatcaat aaccagtttg
     2041 tgatctctga agaatattac taaagttaaa atgtaatccg atttaaatat cgagtctcct
     2101 tgtttcgact taagctggca attggattgc cagctttctt ttaacgcatt gaaattacct
     2161 gagtaaataa cgcatttatg tcttcaggtt tttatatagc gtctggagac acggatattt
     2221 atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg gattacttat ttaaccgccc
     2281 atagcatcaa cgcttacgga actctttgtt cactttccca cagcactttt aaaacattaa
     2341 acctgactac ggaaaatatc agccatgata atgtttgaaa tggctaattt gccatggagt
     2401 gaaaaaaatt agatgaaatt cagtaggttg aaataatcac tagcaggtaa ttatttcaat
     2461 gatagtgcgc aattgatcta caacactgcg tagcggagag agtattaatc ggatcatagt
     2521 cacatcaagt gactatgatc cgggtgacaa ccggggtaat tattgctgct taacgaacaa
     2581 actggcgaag ctgaacaggc tggcggcgct aaatatcagt tcaattccca ccagtgtgga
     2641 aaccagcgtt acagacacca tcggcgttgc accaaggaat atccaggcaa tgacgatatc
     2701 cagcacacca ataacgagct gtagccagct gcctttcatt gaacgctgac gataccaact
     2761 catcaggcga ataacccctg caacacagaa caaaccggca ataaatgccg caatggcaaa
     2821 aatgcccagc tccggtgcac ggatgaagaa atagccgatc aataaatagg cgactgcgac
     2881 gaggaaaccg gataataccg gccagaaatt atgactgcgg ttgctgaata acccgacaat
     2941 aagcgcaata cccgagcaga ttaataatgc acccactact gtgcttaaaa tatcgccaga
     3001 gacgaacggg aaactgatac acagcaaccc gacgataaac agcagcacgg caataaactg
     3061 gattgctctg cgatgctttt tcagcatctc cagatcaaac ttcaaaattg ttgccttatc
     3121 tatatataac atagaaccac cctataaaat taagaagaaa atcccctgct atcaatctat
     3181 gccaaaaacg cgtctaagaa tgcagtcgat ttaataaaaa tttcctaatt gcagtatctg
     3241 atgcatctgt aactcattgt attgaaataa aaatatctga ttttgatatt ttccatcaac
     3301 atgacatata cagaaaacca ggttataacc tcagtgtcga aattgattcg tgacggctct
     3361 ttcactttat agttgaggat attacgatga aaaaagtatt aggcgttatt cttggtggtc
     3421 tgcttcttct gccagttgtg agcaatgcag cggatgcgca aaaagcagct gataacaaaa
     3481 aaccggtcaa ctcatggacc tgtgaagatt tcctggctgt ggacgaatcc ttccagccaa
     3541 ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa accagaagat gcggttttag
     3601 atgttcaggg tattgcaacc gtaaccccag ctatcgttca ggcttgtact caggataaac
     3661 aagccaactt taaagataaa gttaaaggcg aatgggacaa aattaagaaa gatatgtaat
     3721 tccgggaatg cgttacatcg tacttccttg catattgaac aggccggaat atcttcttta
     3781 aaagcagcta ttcctcctgt tcatatataa tctctatatt gaatgggtta caaaatgaat
     3841 atttcatctc tccgtaaagc gtttattttt atgggcgctg tagcggcttt gtcactggtg
     3901 aacgcacaat ctgcgctggc agccaatgaa tccgctaaag atatgacctg ccaggaattt
     3961 attgatctga atccaaaagc aatgaccccg gttgcatggt ggatgctgca tgaagaaaca
     4021 gtatataaag gtggcgatac cgttacttta aatgaaaccg atctcactca aattcctaaa
     4081 gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt ataccttcaa aaatcaagca
     4141 tctaatgact tgccgaatta atgaggtaca agtaaaaagg agtagcaagt tgagccatct
     4201 tgctgctcct ttttgcattt ttatatgaca gcagaattaa ttatacgtct tatactcgcg
     4261 gcaattgcct gtggcgctat tggcatggaa aggcaaatgc gcggcaaagg agcagggtta
     4321 cgcacacatg tattaattgg catgggaagc gccctgttta tgattgtttc gaaatatggt
     4381 tttgctgacg tgctgtcttt agatcacgtc ggactcgacc ccagccgtat tgctgctcag
     4441 gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc tcgttcgtaa ccaaaatatt
     4501 gtaggtctga cgacggcagc ggatatctgg gtgaccgccg cataggtatg gttattggca
     4561 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
     4621 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
     4681 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
     4741 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
     4801 caacgttggt agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
     4861 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
     4921 gataatcaat atgctgatgg cgtaccagct cattgatccg tttaagattg aggatcatca
     4981 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
     5041 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
     5101 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
     5161 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
     5221 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
     5281 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
     5341 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
     5401 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
     5461 gacattaact ttataatcag tttgccaaag gtaattaaca ggacagggct tataataata
     5521 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
     5581 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
     5641 gtagtaaggc gcacccaagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
     5701 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
     5761 ttccaggaag ttatacggga ctttattgat aaagcctggt tgttcaccct gaatggtacc
     5821 gaggatggta acaaagtgat tacgatagtt cactggatca aggaagccgc tttgtctggc
     5881 gagcagtcgg ccctgatagt tggcttcaat atcaggcttc gcatagctat ccaacggtaa
     5941 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
     6001 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
     6061 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
     6121 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
     6181 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggtgat
     6241 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttctgttta gttcaattcc
     6301 gttattttgg ggcgtggtat gagtatccat cgcccaataa tataaccctt tgttttaaaa
     6361 ttacataaaa tctattttta ttcaatgatt ataaagtgct aataatcaat attatggtat
     6421 tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt ctatttccac
     6481 cccataattt atatcttttt cattaggcag atctctcttt aaataaaagg ggtatgaaat
     6541 cttttcgggt tgaagtgtct cgtaatgaaa tgctatgaat tcatttttag acattgttgc
     6601 ccatttttga attttattgt atagagaggg tgttgtaaac tcttgaagtt gtttataaca
     6661 tatttttttg aactcttgct tatattttaa tatgtcatca aatgaaagat tcactacctt
     6721 tgcctctaag aaatcaatta ttctcgaagc aatttccgct ttcgcgcgta aagagttgtc
     6781 attaatagtt aacaatgtag aaaacaagcc attgatttgt ttgtcaagat gttgttcata
     6841 tttatgtagt ttggggatgg gattttcctt gtgtgaaaaa atagtgttaa ataaatttgc
     6901 gtcagtgaaa tttatattaa gtagatcttc aaaataaaat tttgataagg ttaggactag
     6961 cttggacata tcttgccctg tcatgttgaa atcaataaag tcgcatccag caagggcttc
     7021 catcctattt tcatttttac tgtgctcaat atacagcttc gccattttta agtacaagct
     7081 ttgaatgtct cttatatcgg ttggtaagtc agctcgttgc ccagcaaaat ttttattatc
     7141 ggaagaaacg gtttggacac tgaatctttc gggtattcct tcttctacga gaattttagt
     7201 tatgattgga ttgttgggtt ctggattaac gacaacaatg atttgacata cagaatcctt
     7261 atccttttcg tatctaatgt tatgaatctg attaagtatt gattgtgtct gttgcgtaag
     7321 tggtgtcgaa cttatcgttt caatgttatt aataagactt acaagttgaa ccacaacttt
     7381 tgagtagtct gcatttgtat atgtggtgtt cgtgtggcat gcattaattt gttggagttg
     7441 ttgttgcgct aaatcaaatt gttcagggtt gtatccttct ccagagaatg tggaattaat
     7501 gggttgttgt tcttcccaat tatcataagg ccacttcggt gtgtctggcg ttttaacatt
     7561 atcggtattt gttgtgcagt tataaatatc gccgtgatat gtatttatta tacctagggt
     7621 aaagtcaatt gacataacat tccttattca tcatctgttt tatgctaatg tttttaaatt
     7681 ggataattag aacaggaatg gattttcttc tacacacaat aaatataatg cgttatatct
     7741 tcctgatata attaccttta aattcccttt catttccacc cggtaatatt tgtgtataaa
     7801 aaaagggcag tgatagtatc gtaaaggaaa acgttaacaa ctatcaaaaa gcgctcattt
     7861 taaaaagtta ttatgttctc cgatagtgtg tgaagtaata tttttgtgat gttttagatg
     7921 tgtaataatg aaccaggtta aagtgtattt aaaaatgtgt tgtattagcg actgaaatct
     7981 gccttttaaa aaggaggagg gtactgagac aaagagtcat tgcttctccg ataaaatgtc
     8041 agagaagcat gattacgtgg ttattgtctg ttgatgactt gttgaccgag ccgtcatcat
     8101 tctcaatgca ttagttaaat catttgtgat ttttaaggat tatc
//
LOCUS       DAJTRQ010000155         8132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000155 DAJTRQ010000000
VERSION     DAJTRQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            322..3171
                     /locus_tag="OO332_003630"
     CDS             322..3171
                     /locus_tag="OO332_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5943950.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3271..5190)
                     /gene="dhaR"
                     /locus_tag="OO332_003631"
     CDS             complement(3271..5190)
                     /gene="dhaR"
                     /locus_tag="OO332_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5943951.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5418..6488
                     /gene="dhaK"
                     /locus_tag="OO332_003632"
     CDS             5418..6488
                     /gene="dhaK"
                     /locus_tag="OO332_003632"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5943952.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6499..7131
                     /gene="dhaL"
                     /locus_tag="OO332_003633"
     CDS             6499..7131
                     /gene="dhaL"
                     /locus_tag="OO332_003633"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5943953.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7142..>8132
                     /gene="dhaM"
                     /locus_tag="OO332_003634"
     CDS             7142..>8132
                     /gene="dhaM"
                     /locus_tag="OO332_003634"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5943954.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAA"
BASE COUNT         2111 a         2018 c         2040 g         1963 t
ORIGIN      
        1 taggattatt acaaaaaatc ttcaccaaat tttttaaccg ctgtttgttg taatattttt
       61 gccatattca tagtaacgaa ttggaataag taacgacaac ttagggaata actcgatata
      121 aactcacctt tgtaaggggg gtgttaaata tatattaatt cctgtgttcc tgattttgcc
      181 cggtaataga gaattattct cagagaagct catcatgatt gcaccataca gatatcatga
      241 tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag acccatagcc
      301 tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac caaagccgat
      361 cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt tggcgctatt
      421 ggtttgaatg caattatctt ttcccccctg ctgatagctg ctgatacagg aagtcaatat
      481 ggcaccaata ttactattaa tgatggtgac agaattacag gagataccgc cgatccatca
      541 ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat caacctgggt
      601 aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat cattattcag
      661 ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg tcaaacctct
      721 gccatcggca ttaacttaat tggtgactat acccatgctg acttaggcac aggcagcacc
      781 attaagagta acgatgacgg catcattatt gggcatagct caacactaac agccactcaa
      841 ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg caccagtgtc
      901 gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta tatcggtggt
      961 ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct gacaatcgat
     1021 gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt cgatctcgga
     1081 acaaacagta ccattaaaac caatggcgat aatgctcacg gcctctggag ctttggccag
     1141 gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg cgtcgaagtt
     1201 cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca gggcggtggc
     1261 ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc gcaacgaaac
     1321 agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc tgttatcaac
     1381 atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg tttgtgggcg
     1441 ctcagcggcg gtagaataac cggagacagt ttggctatca ccggcgcggc aggagccaga
     1501 gggatttatg ccatgaccaa cagccagatc gacctcacga gcgatctggt cattgatatg
     1561 agtacacccg accagatggc catcgcaacg caacatgacg atggttatgc caccagccgc
     1621 atcaacgcct cgggtcgtat gcttatcaac ggtagcgttc tttccaaagg tgggctaatc
     1681 aatctggata tgcaccctgg gtcggtttgg acaggttcct ccctcagcga taatgtcaat
     1741 ggcgggaaac tggacgttgc aatgaataac agcgtctgga acgtaacaag taattctaat
     1801 ctcgacacgc tggcgctgag ccattcaact gtcgattttg ccagccacgg gtcaactgcc
     1861 ggcacattta ccacattaaa cgtagagaac ctgagcggta acagtacctt tattatgcgt
     1921 gctgatgttg ttggcgaggg taatggcgtt aataatagag gggatttatt gaatatcagc
     1981 gggagtagtg ctggtaatca cgtattggct atccgcaacc agggcagcga ggccacaacg
     2041 ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt cagcgcgtct
     2101 tctcaggttg agttgggggg atatctgtac gatgtgcgta aaaatggcac taactgggag
     2161 ctttacgctt ccgggacagt tccggaaccg actcctaatc ctgaacccac accagctccc
     2221 gctcagcctc ccatagtcaa ccccgatcct acgcctaaac ccaccacgac cgcagatgct
     2281 ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg tacgctgatg
     2341 caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt gcgcagttat
     2401 gggggaagcc tggactcctt tgccagtggc aaactgagcg gctttgacat gggttacagc
     2461 ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta tgtcggtctg
     2521 tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac cgcacgttca
     2581 gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag cgatctcgtt
     2641 ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa caactgcgtt
     2701 aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca gaggttcaac
     2761 ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac atacagccac
     2821 cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa tcactacgaa
     2881 tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg caacagccag
     2941 ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac cgaatatctg
     3001 ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa cggcgtggga
     3061 gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta cacgcagggt
     3121 aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta agtgcgggat
     3181 agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa atacagatca
     3241 ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact gccctgcatc
     3301 aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa gtaaagccga
     3361 catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct ctttttcaac
     3421 ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg tcgcctgctc
     3481 ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac tactcagagc
     3541 aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac aagaaactag
     3601 gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt caagactgcg
     3661 taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag gcgggatggt
     3721 aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt gttccaccag
     3781 cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta ttaaacgccg
     3841 cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag actgtaactc
     3901 caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca gttccagccg
     3961 actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt ccgccagcgc
     4021 agcatcacca tataactcac aattgacggc gatataagga cctgcagcac gctcgctttc
     4081 attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac aaagcaggac
     4141 aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg tttgcggatc
     4201 gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg tcattaactg
     4261 ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg tttctattat
     4321 cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg ttgcttctac
     4381 gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta acgttaagag
     4441 ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg
     4501 ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat catccatact
     4561 ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca aactgtccgt
     4621 cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt
     4681 agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct tgctgtcaaa
     4741 caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt gatcggccat
     4801 cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac caataattcc
     4861 ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca aggtttgcgg
     4921 atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg cacactctcg
     4981 cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca gcatcgcttt
     5041 cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta cgttccatgt
     5101 ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc
     5161 ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca aaaggtgttc
     5221 cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca tttattaaag
     5281 catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc ctgatgcaca
     5341 atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg ccctaccgta
     5401 attgctggcg caaaataatg aaaaaattga tcaatgatgt gcaagacgta ctggacgaac
     5461 aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat ccggtgtatg
     5521 tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt ggcggcagcg
     5581 gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg gcctgtccgg
     5641 gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg caaattgatg
     5701 gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt aactttgaaa
     5761 cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc attgatgacg
     5821 acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc aacaccgtat
     5881 taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac gcctgtgcgg
     5941 aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc ggtgcctgta
     6001 ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg gagtttggcg
     6061 tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt gatcaaaccg
     6121 tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact ttgcgtttct
     6181 gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg ctccagtctg
     6241 gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct gagctgtacg
     6301 gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc gaacgtaatt
     6361 taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc ttactgaaag
     6421 ttgatgacga aacgctggct ctctgggacg tcccggtcca caccccggcc cttaactggg
     6481 gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg gctcactcgt
     6541 tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg cgagattggc
     6601 gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga aaaactccct
     6661 gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac gctgctttcc
     6721 agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc cgcacaggcg
     6781 acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg cgatggcgcg
     6841 gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg
     6901 gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt tccggtggcg
     6961 ctcgaagctg ccagtagcat cgccgaatcc gcggcacaaa gtacgattac gatgcaagcc
     7021 cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga tcccggcgcg
     7081 acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta aggaattggt
     7141 gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg tcggtgaatt
     7201 agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg gaattgacga
     7261 tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg aatctgttgc
     7321 tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga gtgctgaaac
     7381 tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt
     7441 ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata tcgacaaagt
     7501 tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt taccgtcctc
     7561 cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt ctctggcggt
     7621 ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg tttatacctt
     7681 atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca caccagagag
     7741 tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc tgattgcgaa
     7801 agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag ataactttgg
     7861 tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt cgggtaaagc
     7921 cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga ccgcggaaga
     7981 agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga tgacgttaac
     8041 agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg gtcaccatac
     8101 actgttaggt gatccggaac tgctggcggc gg
//
LOCUS       DAJTRQ010000156         7940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000156 DAJTRQ010000000
VERSION     DAJTRQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..167)
                     /locus_tag="OO332_003635"
                     /pseudo
     CDS             complement(1..167)
                     /locus_tag="OO332_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            165..489
                     /locus_tag="OO332_003636"
                     /pseudo
     CDS             165..489
                     /locus_tag="OO332_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(519..3431)
                     /locus_tag="OO332_003637"
     CDS             complement(519..3431)
                     /locus_tag="OO332_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943955.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3434..3550
                     /locus_tag="OO332_003638"
     CDS             3434..3550
                     /locus_tag="OO332_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5943956.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3914..4756
                     /locus_tag="OO332_003639"
     CDS             3914..4756
                     /locus_tag="OO332_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5943957.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4759..5847
                     /gene="capU"
                     /locus_tag="OO332_003640"
     CDS             4759..5847
                     /gene="capU"
                     /locus_tag="OO332_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5943958.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5852..6802
                     /gene="virK"
                     /locus_tag="OO332_003641"
     CDS             5852..6802
                     /gene="virK"
                     /locus_tag="OO332_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5943959.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6867..7811
                     /gene="lpxM"
                     /locus_tag="OO332_003642"
                     /gene_synonym="msbB"
     CDS             6867..7811
                     /gene="lpxM"
                     /locus_tag="OO332_003642"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5943960.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2138 a         1778 c         1887 g         2137 t
ORIGIN      
        1 accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt ggaggcactt
       61 ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg ccatgaaagc
      121 cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt cagcggcgga
      181 cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt tacccggtca
      241 aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg ttaccacttt
      301 aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg
      361 aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa tctgctggag
      421 caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac gtacttacgt
      481 acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg agcggcacga
      541 cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa acccttcttc
      601 cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg caaatcatgc
      661 cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc ctgccttatt
      721 ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag cttttttcca
      781 taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata actccttcgt
      841 ttgcggcgtt ccggcagcaa tgcttcagga agatgaacca cacactctgc cagtcgtgcc
      901 gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa cagcgcctgc
      961 atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag gcgtccttcc
     1021 acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact tttctgttgc
     1081 gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc cagcccgtca
     1141 ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag tgcaatatta
     1201 cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg atcaacacca
     1261 taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg ccggaaatac
     1321 ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt aaaatcttcc
     1381 cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc cagcgcctgc
     1441 agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg tcgtgcatcc
     1501 tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct acggatggtg
     1561 tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg ccactcatca
     1621 cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag cgcaagcaga
     1681 ctccacagag gatgctccac ctgctgttca acaagataca cattgtgtgt agcacgggtg
     1741 agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg atcctgcctg
     1801 tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag accctcgcaa
     1861 atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac cgtttcgtat
     1921 tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca gcatcgcgcc
     1981 tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag tgaggttttg
     2041 gcattcagtt cctgctttgt ctcttccctg tcgtccagca accggatgct cccttctgcc
     2101 tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa gcggacctgt
     2161 ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg gtaattcgcc
     2221 tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag acttttgagc
     2281 gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca gagtaaaaac
     2341 tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt catatcctga
     2401 acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata ctgatgactg
     2461 agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg ttcaaacagg
     2521 acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc cagtgacaga
     2581 tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac gcccctgaac
     2641 tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg gggcagccag
     2701 tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt ttccagaaat
     2761 tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga agcgtcatag
     2821 agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat atcaccagca
     2881 gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga gccaacaatc
     2941 accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga cagcatttta
     3001 tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc agtcgcgata
     3061 tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac cacgtttctg
     3121 cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac aaggcagata
     3181 gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact gagccgggcg
     3241 cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc ggtggcaata
     3301 gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg ataaagacag
     3361 attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg aaggaatgcc
     3421 ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc agaaaaatgc
     3481 tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc cgctggaaat
     3541 ggcgcagtag ctgcaggcct gtcatgatgc cggaatagaa gttcgtgtac aggttaatga
     3601 aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg gagcacctgt
     3661 gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg tgccacctca
     3721 aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta tgaattgttg
     3781 atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta ttcatccagt
     3841 atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc ttactaggtg
     3901 ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc atgttccggt
     3961 attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc ccgtgacatt
     4021 ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat cagatgaact
     4081 ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga catttgatga
     4141 tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta atctcaaagc
     4201 acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact ctcctggaaa
     4261 ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg ataatgttat
     4321 gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat ttcatgttca
     4381 tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac agtgtaagca
     4441 tcttcggcag gacttgttga gcgggagaga atatttgaaa gaaatgacag gaaaatgcag
     4501 taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg tcgctgaaga
     4561 acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg ctaaaggaac
     4621 tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc tgaaaagacg
     4681 acttttttat tatacaacac cgtttttttc atcgttgctg gcctttcata aggggccaag
     4741 attaccggat gattaataat gaatatacta tttacggaat catcaccgaa tataggtggt
     4801 caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca ttcagttctg
     4861 cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg aattgatatc
     4921 acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt actcggaata
     4981 gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag caatattgtt
     5041 ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca aaagacatat
     5101 ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt gattgttccc
     5161 ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt tactgttgtg
     5221 cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt gcctccaaat
     5281 gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt aggaatgttg
     5341 cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa aatgaatgga
     5401 cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga gcatttacag
     5461 tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa tgtttttcct
     5521 gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa cgaatcgttt
     5581 ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag tcagattggt
     5641 ggaatccctg atgttattca gaacaaccag accgggacat tgttaccagc aggtaataag
     5701 cacgcatgga tgtgcgccct gaatgatttt tttaatgaac ctgggcgttt ttatcagatg
     5761 gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac tgcgttaaaa
     5821 atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact tatcatttat
     5881 cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt gggaacacag
     5941 aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca gtattcgata
     6001 ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac aacccacgtt
     6061 gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt cccgggggca
     6121 atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt ctgaattttt
     6181 gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag aggattttga
     6241 tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt cattgttttt
     6301 caatcaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa atggacacac
     6361 cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgatg ttattcggtg
     6421 cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag cattttgtgc
     6481 gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca gtcatgtttt
     6541 ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct acagtgattt
     6601 ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa cacaggtggt
     6661 tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa aacgatacgc
     6721 tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc ctgtaaactc
     6781 agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat taatgatgat
     6841 tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt attcctgaat
     6901 ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg ggaatgattg
     6961 caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa atagggcgtt
     7021 gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta tcgctttgtt
     7081 tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt gccacagcat
     7141 tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag ttccagaaac
     7201 gagtgttctg gaaaggcctc gaaattcttg aggaaatccg gcacaataac agaaatgtga
     7261 tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg gcagctcagg
     7321 gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat tatgtctgga
     7381 attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg ataaaaccat
     7441 ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag gatcatggtc
     7501 ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta ccaattattg
     7561 gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt tatgatgaaa
     7621 aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt gccagcgcgg
     7681 acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg gggtcacatc
     7741 cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac gaagcggacc
     7801 cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac aggttaccaa
     7861 tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt gtagatgcag
     7921 ttcgttaaat ttaatttatg
//
LOCUS       DAJTRQ010000157         7895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000157 DAJTRQ010000000
VERSION     DAJTRQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.158"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..800)
                     /locus_tag="OO332_003643"
     CDS             complement(<1..800)
                     /locus_tag="OO332_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5943961.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTR"
     gene            complement(797..1675)
                     /locus_tag="OO332_003644"
     CDS             complement(797..1675)
                     /locus_tag="OO332_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5943962.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(1686..2593)
                     /locus_tag="OO332_003645"
                     /pseudo
     CDS             complement(1686..2593)
                     /locus_tag="OO332_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(2580..2813)
                     /locus_tag="OO332_003646"
     CDS             complement(2580..2813)
                     /locus_tag="OO332_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943963.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(2810..3667)
                     /locus_tag="OO332_003647"
     CDS             complement(2810..3667)
                     /locus_tag="OO332_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5943964.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            3692..4021
                     /locus_tag="OO332_003648"
     CDS             3692..4021
                     /locus_tag="OO332_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5943965.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4011..4319
                     /locus_tag="OO332_003649"
     CDS             4011..4319
                     /locus_tag="OO332_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5943966.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4361..4537)
                     /locus_tag="OO332_003650"
     CDS             complement(4361..4537)
                     /locus_tag="OO332_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR5943967.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4534..4944)
                     /locus_tag="OO332_003651"
     CDS             complement(4534..4944)
                     /locus_tag="OO332_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5943968.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(4941..5219)
                     /locus_tag="OO332_003652"
     CDS             complement(4941..5219)
                     /locus_tag="OO332_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5943969.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5294..7642)
                     /locus_tag="OO332_003653"
                     /pseudo
     CDS             complement(5294..7642)
                     /locus_tag="OO332_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            complement(7682..>7895)
                     /locus_tag="OO332_003654"
     CDS             complement(7682..>7895)
                     /locus_tag="OO332_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5943970.1"
                     /translation="NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLL
                     ERLGHIPPAEAEKAYYASIGNDDLAA"
BASE COUNT         1880 a         2146 c         1806 g         2063 t
ORIGIN      
        1 aaacgcgtcc agtcttcatc ttcaaagtga gccacagatt tcaggcgtga tactggcatc
       61 cctccagccg cagacaccat acgttcacca atctggatgt tcgccatctc catggtgaac
      121 agaagcacac catgcccctg ctcagtcacc ttgtcgatga tgtccagcgc aagttcggtt
      181 ttccccatcg aaggacgggc ggcaatgaat accaggtctc cgggctccat accgcctgtt
      241 tttgcgtcca gttcatcaat accggtcatc aacgtcctgg atttctccag cccctgattc
      301 cggcattcaa cacgctcaac cacttccgga agcacatcat caatatgtac cggctgaatg
      361 acgccctttc ctgtcgacag tgtgaccatc atgttctgcg catccttcag ggcatcttca
      421 gctgcttcac aggtatgcgc atcacgtaat ttctgcagcg cctcattcag tgttttttct
      481 gcattgcgca gtgcggcatt gcgccgcaac gctgcaacat agtgctccag tgaagacttc
      541 acccaggttt tacgcccggt atcagtaatc accggggcaa gttccggcat ctcattacac
      601 aacagcacgg ggtcaatcac tcctgaaaca cgggcctgtc tgcagatgcc tgtatagata
      661 tcctgatacg ctcgtacaga aaaaacgtcc gctggcagtg tggccagaat atccatcact
      721 tcaggatctg ctccgcgcag aaagaacgcg ccgatgacag cgccttcaag gtcatcgtta
      781 cgccatactg gagttgtcat gcagccacac ctctgatacg agaacggtaa ctgggccagt
      841 taaacgacaa ccagttgcgt cccccgtctg tgatcctgtc ggcaattcgg ggactgatga
      901 acgcccacaa ctcttccggt gagaggttac tgatcagaat ggtgggcaag atactttcgt
      961 acctggcgtt gataatttcc tgcaaaatag ccatttcagc cgcgctgcca aactgaacgc
     1021 caacttcgtc gatgatcagc aaatccattg acgcataacg ctcaataact tcatccgctg
     1081 ttttttcgct gtcattccgc cagcaatttt tcacagcacg ggtaaggcgc atcacgtcgg
     1141 tgatctccac actggccaga tagttacgga tgatgtgttt tgccatagcc acagccagat
     1201 gattttttcc ggtaccacaa ctgccggtca taacaagact ggtaccgttc tccagcatat
     1261 ctggccaatt ctccgcatag cggcgacagg ccgcaagatt tctggctgcg ccaggattaa
     1321 cctccagata attatcaaac tcgcagtccc gaaaacgcag ggcaattccg gcgttatcag
     1381 tcagctcttc cgccttgatg gacgacagct ccatggtcaa atcgttggcc tcagcgatta
     1441 agcagtcagg gcagcatgaa attttttctc tgtcctcgcc attacgatcg ctccacacca
     1501 gtatatgcgt gtgatattcg ccatgttttt cgcaatatcc gcgaccttca cgcatcaggc
     1561 aggaacgata aggccatggc ttttcgccct tctgagcaaa tgcaatctct gcccgtaact
     1621 catccattcg cgcctgtagt cttttttgtt gttcacgcag gttaaacgtc atcatcgctg
     1681 tcacctcaga atgtcagttt gtcactggat ttaccgaatt tgtcagacat ggctcccagg
     1741 ccagccagga catcgacctg tcgctgtcgc ccacctccgg gagcggctgg ctgttgccag
     1801 aagtcttcga agtgacgatc gggtccaaag aacgtcgcag cctgcttcac gaactgtgtg
     1861 ccggtatttc ctgtggcacg tacccaggca gcatagcgtt tcacgccatc aagcatggct
     1921 tcaggtttta ttccctccct gagccgggct ttccaggctt tgaaggctgc ggattttgag
     1981 ttaccaccag cacgttttgg atattcctgc caggcctgtt caaattccgg tgaatattcc
     2041 tgtcgggaag aacgcgctgg tgcagacgcg tcagcagatg cgccaatagt gtttttactc
     2101 tctgtagtat tctctgaagt aatctctgtt gtattctctg taagatcgaa attggttttc
     2161 ccttctccgc ggcgaggggt ttcccgtgtc cgcggtgaag gctttccctc ctccgcgaaa
     2221 ttgggtttta cagtttcccg aaaacgggtt tccccatttc gggaaactga ttgttttcat
     2281 tgataatttc attaaggcgc tcacaatcta tacggtagaa cattttgtgc tcaagacgct
     2341 tgttggtttc aaccaaaatg cctctggaca caagatgctt acgcgctaca gcctgttgtt
     2401 caaatgtaag tccggtttcg tgttgtatct cttcacgcgt tttatgtacg ccttccgctg
     2461 catgtgcttt atcctaccag taaaaaatct gaccaaagaa aataacagcg tgcggacttc
     2521 ccatgtattt aacgagccca gggtaataag caaccggatg tccaaaatcg agcagaagat
     2581 cagacggacg catagccacc tcccaggcgt ttaaacattt ttccgggctg aaacgccacc
     2641 agcggataac tcagggtatg agtacgtccc tgaacctggc agacaacctt ctggctttcg
     2701 gtactgacca ggcaaacccg cagaacgtag ccgttgctgg tggtgaacca ctgccccaca
     2761 cgggggcaat ggttgtatcg gtgatacagg aaattaacga tgtggcggat catgggagca
     2821 cctccttgtc agaaccattc agcctggaat caacaagtgc agcaccaaaa acagcatcac
     2881 ctacacggtc gtacagtttg ccagccagcg gagattcaac ggccttaagc attgggtaaa
     2941 gctggcttgt ccagatttga tggatttcac gcaaatgcag gtatacgcct ctggcgtttt
     3001 gtgcgacaga tgacatgtca gccgcaccag ctcctgataa gctcctctcc atctggttaa
     3061 aggcattgat gtatgcctct ttgaaccggg cggcacgttt accagtgaag cccatagcaa
     3121 ggaaggcgaa gccgtcgcgg gtaatttggt agcagggaag tttgcgagta ccgccgttgg
     3181 gctggcgtac caaaattgat gtctccgcaa aattgcgggc acaaaactct ggagaacaat
     3241 ccaaaatgcg gatctttttc agaacatcgt catgacgttt agagaagaag ttggcaacag
     3301 ccagggatga agtaacagcc tgaccatcaa cgatggcaat ttcaggttga gagagggttg
     3361 ggagagtagt catggtgaca gcccctatgt tgaattcaaa gaactcacca catgggacgc
     3421 caatcacaga ggtggtgaga cgtacagggt tggcgtaacc ggtcaacata gaacccggcg
     3481 catcttgcga tgcccctgca cgccccacca taatttgggc gtagcaatgc tcatgacacg
     3541 aaaaaaccgc atgagcgcgg ttatgctcta tattgaattt caggacgcca atcccggcac
     3601 ccgctttata aggtgcagag acagtgtaac gtcccgaaat tgcagaatca atatattgtg
     3661 cagatataac gattttgtta tgttttgctg tatgaactac actatcgaat actacagtga
     3721 agaggtccga ctggaggtcg atcagcttcc attgagtatg cgtgccagat accagcatct
     3781 tgttgaacgc atgaaggtat acggcagcaa tctcggagaa cctcacacca gcgcctttgg
     3841 tgacgggctt ttcgaactca gaattaaagg caacgatggg atcgcgcgtg ttttttactg
     3901 cactctgaca gggaaacgca tcatcatgct gcatagtttt gtaaagaaaa cgcagaaaac
     3961 accgccagcc gaacgcaaga aagctgaaac cagaatgaag gaggttaagc atgactggta
     4021 aacgcactct ccccaccatg acacacgacg aaatggcagc caaatggatg gaagacccgg
     4081 cttttaaagc agaatacgac gctatcgctg acgaattcgc actgcttgat gaaatgctgg
     4141 cagcacgcaa agaagctggc ttaactcagg ctgaagttgc cgagcgaatg ggaacaaaag
     4201 cgaccgcgat caccagaatg gaaagtaatc tcgcatcagg tatcagcggc ccatcatttg
     4261 ccacactaaa aaaattcgcc cgcgctactg gaaaaaaact ccagatccgc ttcgtttaac
     4321 cacaccgcgc cgtcattctg gcggcgcacc ggataaatag tcatttcctc gcacgatgtc
     4381 ttagccaccg gatatcccac aggtgagccg tgtagttgaa ggtttttacg tcagattctt
     4441 ttgggattgg cttgcgttta tttctggagc gtttcgttgg aaggtatttg cagttttcgc
     4501 agatgatgtc ggtgaaactt cgtcgctgtc gcctcattca tacctcctgt cggtaaatct
     4561 gacaccctga ccaatagccc aggctgttgt gtactcgatc agacttgcca tgcgcttcac
     4621 gctcatctgc gcgctgcttt cgcgaatgtt gacgcattct ccttcaaggc cgggcaaaac
     4681 atcagcttcc tgttttgttg ccactgcatg accgctgatc aacaaaacct tccattgttc
     4741 tggttttaac catttgccgc accattgaac ctgacgagcg atatccgcca gcatcgcgtg
     4801 aaattttgcg ttctggtcaa gatttcgctt gtagtcagta atgcggatgg tgactggctt
     4861 gtctttatcg agaggagttg agaggatggc atttattgcg gcttgctgtt gttgcttagt
     4921 tcggaggaag attgtttgct tcatcgaaaa ttcttctctt taattccagc ggctctgata
     4981 gctttcatta ctgcaattac cgttttgtcc ttcccatcct catgccccat cgcataagca
     5041 ccttcttcac catctttcca aaagtcgtca ttcgattcgg gccagtcgat atccagttca
     5101 atagctgctc gcggtgcctg ccatatcacc caggcaaact cttttaattc atcgtctcct
     5161 gtgaactggc ttttgtcttt tgaccaccag ttttcaaact gtcggtagct atcgttcact
     5221 tccctctccc ccaaataaaa aggcctgcga ttaccagcag gcctgctatt agctcagtga
     5281 tgtagatggt catttaatac tccgtcacgt tttcctgtcg ccacgcctcg tcatattccg
     5341 atttcggcat attggcgatg tagctatatg gcgatcctga ttcaagttgc aggaactggt
     5401 gcgattgctc gtcaaggaac aacgggacac caccttccca accttcgccg ttacgttgtt
     5461 tttcaagcat caaaacagat gccggagatg ccagtagctg ttcgtccttc tctgacatct
     5521 tttcaccact ctgaactctc tgtaacgctc tctcgcgagc cttgttacgc cagatgatga
     5581 aaaggttgtc tgtcaggtct gttatcgctc cagagccttt tacgtccatt ttcccggttg
     5641 gtttttcttc gctgtctcct tttcgcgagt gagtaacgag aatgacgtgg gagtttgttt
     5701 tgtttttgaa gtcgcaaatc gagtcaacaa acgccttctg cccgttatag tcatcgtcgc
     5761 ctatgccaca tttcatcagg ctgtcgatga tgaataactg gatcccgtat cggcggcgag
     5821 cgtagtcgaa tatttcgatc agcctgtcgg ctttcgccgt tccagtcagg ccaaacaccc
     5881 aaagtctttc gtcataaaat ttaaatgcag agtcaatttc cagcactggc ggcatcttgc
     5941 agcacgttgc ctgacgggta agtcgcttaa ggagaatacc aggcttcagc tcaagtgacg
     6001 cgatgcacgt cttcacaccc tgacgcattg cctcaagtgc catatgcccg acaacctcgg
     6061 tgttatgcgt cgccacatat cccctggtta catacaggtg gcgagggtga tcgaccataa
     6121 tacacaagca ttctgcattg ccgatttttt caacattcct gacgaatacc ccacagccct
     6181 cgaatcgatg cgcagttaat ctgccattga gtcgtggtga acggatggcc tccctgattt
     6241 ctcttgtcag tctgattctt gcctcgtatg aatccatccc atgccgctta tcgtccttgt
     6301 atgtgtatgt cacgccagtt ttaactcgcg tccggcatga cccgccgagt gagttaacca
     6361 gttgaacaac cccattgcgc agttcttcac ttgctgagga aaaaacaagc gttccatcct
     6421 tctcgacata cccatccgtt tcaagcagac cacacagcat gcctatacgc gttgatttat
     6481 ttgcggaaaa aaacacacga gggatgaatt tgttttttgc tgtgcacccc attagtccat
     6541 aacctcgcag ggtctccatg agtggattta cctgaccacg cgccgttgat atcagccagt
     6601 ccttaccatc tccagagaag ttgtaatcag gcagttcagc cttcatacgc tcgatcatgt
     6661 atggctcgac gtttgaaaac ttcacgctcc cattgctaag actaccatcc ccgagaaggg
     6721 agccgataac ccatgctaat ggctctgagt ggtcgccaaa gtcaccagtt atttcaggaa
     6781 tcctaacgcc atttttgtgc ctcttcgttt cactcaaccg cttcaacccg aaggtatcta
     6841 tcacgcggcg tttctcgcct ttcgtgaatc cacgactagt gacctcccat aggtgatcgc
     6901 ctgcgcaatc aacataacga ccatcttcaa atgtgactcg gtaaacatct ctaacaccct
     6961 gcgggaatat cccagtgaca gttgacggat tgccgtctac tgacgccacc tgatcgccaa
     7021 tttttacatt cccgtgagta gtccatgtcc catcagctaa aagaataggc tcattcagtg
     7081 ggcatgcctt tccgtgaccg ttcacaccat tgaccagcgt caactctgcc tcacggaact
     7141 ggaatttatc tgccagagat tcccacggtg gattaaacag atactgctgc ttgccgtaga
     7201 aagcgttgat agtgtcctgg taaaactctc gcgcactgta gagttcttca ggatcgaagt
     7261 aggatgccgt gccgatgtac tgccagattt catcctcggt aacaccgttc atcaggcatt
     7321 cgttgatgtc tttgtacggc agagtaacaa gacggcaacg atgttcaccg agtcggcttg
     7381 cgatttccct tgcggcttca cgaccaacat catcaacgtc catcgagatg aatatttcct
     7441 caaacctgtc gaggttatga tactcaaact caatccactg ttgcttagcg cctttcccgc
     7501 caccaaacgg cacggataac gccgagatgc cgtattgcgc atagctcata caatcaattt
     7561 cgccttcgca aagcacaacc gccctcacgc cagcgtccag agcctgccat ccgaacaggc
     7621 aaggttcgca atcaccttct gaaccgcccc gggtttcctg gagagtgttt tatctgtgaa
     7681 ctcaggctgc cagatcatcg tttccgatgg aagcataata agctttttct gcttctgccg
     7741 gagggatgtg gcccagcctt tccagcaatc gtcgattgtt ataccagtcc acccacgtta
     7801 gtgtggccag ttccacttct gcacggtttt tccagctctt acggtgtatt acctccgctt
     7861 tgtaaagacc attgatgctc tccgccatcg cgttg
//
LOCUS       DAJTRQ010000158         7836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000158 DAJTRQ010000000
VERSION     DAJTRQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO332_003655"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO332_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5943971.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OO332_003656"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OO332_003656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5943972.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OO332_003657"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OO332_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5943973.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OO332_003658"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OO332_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5943974.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OO332_003659"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OO332_003659"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5943975.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6421..7050
                     /locus_tag="OO332_003660"
     CDS             6421..7050
                     /locus_tag="OO332_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5943976.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7189..>7836)
                     /locus_tag="OO332_003661"
     CDS             complement(7189..>7836)
                     /locus_tag="OO332_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5943977.1"
                     /translation="SYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGA
                     SILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGEYKL
                     HVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALP
                     TLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT         2021 a         2003 c         1927 g         1885 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gacttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagct aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtaacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg gtcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaattgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagca cctggcagca tgctgccagg tgctgggcca cctcttttgc
     6361 gattgtaatt tcatgcttgc tggtgaatag tcagtatttt ccctgacttg cgaactcacc
     6421 atgacccgca cactcaagcc gttaattctt aacaccggcg cactggcgct aacgttaatc
     6481 ctgatttata ccggcatttc ggcccatgac aaactcacct ggctgctgga agtgacaccg
     6541 gtgattatcg tcgtgccgct actacttgcc accgccagac gttacccgtt aacgctgctg
     6601 ctctatacgc tcatcttctt tcacgccatc atcctgatgg taggcgggca gtacacctac
     6661 gcgaaagtcc ccgttggttt tgaggtgcag gaatggctgg ggttgagtcg taatccgtat
     6721 gacaagctgg ggcacttttt ccaggggctg gtgcctgcac tggtggcacg agaaattctc
     6781 gttcgcggga tgtacgtgcg cggacgtaaa atggtggcgt ttctggtctg ttgcgtggcg
     6841 ctggcgataa gcgcgatgta tgaattaatc gagtggtggg cggcactggc gatgggccag
     6901 ggagcggatg attttctcgg tacccagggc gaccagtggg acacgcaatc cgatatgttc
     6961 tgcgcgctgc ttggcgcatt aacgacagtg atattgctcg ctcgctttca ttgccgccag
     7021 ctacggcgct atggcttgat aaccggataa caacgtgcac tttgttgatg ccggatgcgg
     7081 cgtgaattcc ttatccggtc tacaaaattt gcaaatccaa agcattacat taagacgtgg
     7141 cctgataaac atatcgcatc aggccatttt cgttagctat tgtccacgtc aggctttcac
     7201 atcactcact gcaccgaccg gaatatcagc atgaacaggc tcgggcggta ctttgcgata
     7261 tttatccagc agatgaatct ggatcctgcg cagcgtgtcg ccattgaggc gatagaaacg
     7321 gaagtagaga atcagcgtta ccatgaaaaa cagagtcggt agagcaatca taataaactg
     7381 catacctaat agggcttgcg tagactgttc aacattcggt acatagccaa tcatccctaa
     7441 cacaaccgca atgaaaaaag ccgcaaaggc tgaaccgccc ttcaccacca tagtctgcac
     7501 ggagtaagcg atgctttcac agcgtacgtg cagtttatat tcaccgtaat caacggtatc
     7561 tgccaccatg atgacctgta atacccagaa aagcgccgtt cccacattca gcaaaatacc
     7621 cgcaatcaca atgaggacga cgttgtgata gctcattaat gccattaaca ggagaacacc
     7681 acagcttaac accggaagaa tagatgctcc ggcccataaa atgcgtcggg ataatgattt
     7741 aactaagcgt gggaagaata ctaacgtcac caggttagca gctcccgcat acgacagata
     7801 ataggggaac aaatccgcat caccgataac atatga
//
LOCUS       DAJTRQ010000159         7756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000159 DAJTRQ010000000
VERSION     DAJTRQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..608)
                     /gene="chbR"
                     /locus_tag="OO332_003662"
     CDS             complement(<1..608)
                     /gene="chbR"
                     /locus_tag="OO332_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5943978.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKS"
     gene            complement(616..966)
                     /gene="chbA"
                     /locus_tag="OO332_003663"
                     /pseudo
     CDS             complement(616..966)
                     /gene="chbA"
                     /locus_tag="OO332_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1025..2373)
                     /gene="chbC"
                     /locus_tag="OO332_003664"
                     /pseudo
     CDS             complement(1025..2373)
                     /gene="chbC"
                     /locus_tag="OO332_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2458..2778)
                     /gene="chbB"
                     /locus_tag="OO332_003665"
     CDS             complement(2458..2778)
                     /gene="chbB"
                     /locus_tag="OO332_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5943979.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3077..3415)
                     /gene="osmE"
                     /locus_tag="OO332_003666"
     CDS             complement(3077..3415)
                     /gene="osmE"
                     /locus_tag="OO332_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5943980.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3617..4444
                     /gene="nadE"
                     /locus_tag="OO332_003667"
     CDS             3617..4444
                     /gene="nadE"
                     /locus_tag="OO332_003667"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5943981.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4674..5561
                     /gene="cho"
                     /locus_tag="OO332_003668"
     CDS             4674..5561
                     /gene="cho"
                     /locus_tag="OO332_003668"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5943982.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5521..6096)
                     /gene="ves"
                     /locus_tag="OO332_003669"
     CDS             complement(5521..6096)
                     /gene="ves"
                     /locus_tag="OO332_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5943983.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6299..6784)
                     /gene="spy"
                     /locus_tag="OO332_003670"
     CDS             complement(6299..6784)
                     /gene="spy"
                     /locus_tag="OO332_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5943984.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7114..>7756)
                     /locus_tag="OO332_003671"
     CDS             complement(7114..>7756)
                     /locus_tag="OO332_003671"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5943985.1"
                     /translation="WQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGS
                     LHPQFGVLPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLEL
                     GKALPFGQNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMA
                     SDTLNFMPFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2049 a         1921 c         1793 g         1993 t
ORIGIN      
        1 tgtgatttgg ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta
       61 tcatgcatct tttctaccgt acttttcagc cactgcggta catcatcaat cacctgttct
      121 tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc
      181 agccctgttt cgcggaaatt taatgaagaa atcactgttt ccacataggt gagaaaagcg
      241 ttattggtcc ggtatacctg cgaagcaaca aagcaataag gcaacaacgg caggtaatgc
      301 tgctcaaaaa agcgtttact gatcccaacg ttcaatatgc gcgtggcacc aaactcataa
      361 aaactttgat ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg
      421 cgcttaccgt taatctcctg gaaataacgc ccggttaata ccagagtaaa ttcataatag
      481 tcgtgctgat gcagtccgct gatactatca gttttgttat agataaacac atggaagttt
      541 ttgccattaa acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc
      601 tgcatcatcg actccttatg ccttcagttt ttcatgaagc tcaattaatt cagtaatcag
      661 ttcacgcgca agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac
      721 tttcatctta ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc
      781 attcaatgcc atccgtgact gatccatcat ggctttcgct gcggcaaaat cgccctgctt
      841 cgcctgtttc aatgcggcat acgccaggct acgcgcttgt ccagagttga tgataagccc
      901 catcaccact tcttacagct cttcagcttc tgtttgcgta tcaggaatat tatcgagatc
      961 catcatacat cgttcctctc tcttcttacc ggcaagatta cccgtaccgg catcgattaa
     1021 aatttcaggg gttagcgata tcttcttcgc tctcttcttt atcaatcgca ttttgtgctt
     1081 tgttagccac cacaacaaag ggcagataaa ttaacgttgc gatgccaagg ttgaagagtg
     1141 cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa ggctcccaga ccggttggca
     1201 tggtccacgg tgcaatattg gtcaccggag gaatgatgcc catgtagtac gctgcgaggg
     1261 tgattgccgc cagaatcggt tgtaccagta caaacgggat aaacatcacc gggttcatga
     1321 taattggcag accaaacaga atcggttcgt taatctggaa gatgccggac ggcagcgcca
     1381 gttttgccac ctgacgataa tcagcacggc gagaggcgat aaagatagcc aggatcaggc
     1441 ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga
     1501 tatggaaggt cttaccggct gccagcgccg cttcgacgga accatattgc tgataggtcg
     1561 cgatattttc cagcgcccac ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg
     1621 cgccatgaat accgaagaac cagagcagtg gaacaaagat cacataggcc cagcccacca
     1681 cgctacccaa cgatgccagt ggggttgaga tggtatccat aatgatctga tggaagttgg
     1741 tgccccaggt attcaacgcc caggcaataa tccccatcac ggaaagaata ataaagccgg
     1801 gaattaatgc cgagaaagaa cgcgatactg aagcaggtac gctgtcgggc agtttaatga
     1861 cccaattgcg gcggacaata aaggtaaaca tttctgccac caccaggcca ataataatcc
     1921 cggagatgat attcgcccca cctaaccagt ttgcgccaac cgcataggcc tcaccgacac
     1981 tatatggggt gacggtcata aatgctgcaa cggataacaa cccagccgcc agcgcatcga
     2041 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattattc
     2101 ctaatgttcc gttatatacg ttgccgccaa tacctttcag accattaagt gtttcaatgg
     2161 ttgaggcgtc gaggcgaata cctaaggaat aaaaaacgac ccctccccaa agcttagaaa
     2221 aacgttgtta attaatacaa acatggcccc cgcaagggtt aacggcatta agcgaataaa
     2281 gccatttttg attgcattaa cgtgtggctg ctttcctatt ttaactgcaa aagggaggag
     2341 taccttttca agcgatgcaa taacattact catagaaaaa tacccttaaa aaccgcaatt
     2401 taaatattgc ggtattgatt tatgaaataa ctctttgacg ggaaaattta aaataaatta
     2461 atttgctgcg gcttttttaa tcgctgcaac cgcagcctta agcacgccta aaccatcgac
     2521 tttgccataa agcagcgagt caattacttc aaccggtttg ttgggtaaca aacgctggat
     2581 ttcgggcaac atataagcaa tctgcggccc taataacacg acatcggcat tctgaccttt
     2641 ttcaccagcc agtgtttccg gaaatgcttc aataatgacc ggaacttcat atttttctgc
     2701 ctgtgcgcgc atttttgata ccagtaaaga gatagacatg cccgcagaac aaaacagata
     2761 aatgtgtttc ttttccataa aactgccctc atcgacgatt atctgtcagc cagacactcc
     2821 gcaagcctta acctgcttcc atgctctggg taacttgcga aaccaacatg actttttttg
     2881 tggctgaaag gagtatacgg tatagacctg agcaatagta tttctttgac cttataaatt
     2941 gtttctcctt gacctgtggt tatgaccctc ttcacatttc gggcaaataa ttcgcgaaga
     3001 taattaagaa ctcaggccaa aaaaaaccgg cgcaatggcc ggtttccgtt gttactcaag
     3061 ctttcagacg aattgattac ttcgcagcct gtggatcagt gtcgtattca gcacaggtct
     3121 gataaccgga gttgatgaca tgtccggtat catctaacgc gacaaagtag gtttctgctt
     3181 taccatcacg ttgacccagg atgtaggtct ggcaagtacc gcgagcatgg atcatgctca
     3241 cttcagacga aggtttaccc gcaatttgtg caacctgcgc ccggctcatg ccttttttga
     3301 cgtctttcac cacaggctgt acaaactggt ctttggtacg atcataagct gtacaacccg
     3361 ccagcatggt taataccgcc gctgcactca gaattcctgc catattcttg ttcatattcc
     3421 gtcctcttgt ttatcagcgt gttagataag cctggaatac attgggcgct ttttcaagcc
     3481 cgtgaacgaa acggctccgc tttcagagga ttcctgtatg acgttttaac caccattcag
     3541 cccgctgtcg cttgtcgttt cagtagcaac gggttagctt taaggaagtt ttgtcttttc
     3601 tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg gcgcaaaacc
     3661 gcagattaat gctgaagagg aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca
     3721 aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg actccacgct
     3781 tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc caggaaactg gcaacgaatc
     3841 actgcaattt attgccgtac gcctgcccta tggtgttcag gctgacgaac aagattgcca
     3901 ggatgccatt gcctttattc aaccggatcg cgtattaacc gttaatatca agggcgcggt
     3961 attggctagc gagcaggcat tgcgggaagc aggcattgaa ctgagcgatt ttgtccgtgg
     4021 caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta tgaccagcgg
     4081 tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca ctaaatatgg
     4141 tgacggcggt acggatatta atctgctgta tcgtctcaac aaacgccagg gtaaacagtt
     4201 actgacggca ttaggttgcc cggaacacct ttataagaaa gccccaactg ccgatcttga
     4261 agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtgacctatg acaatatcga
     4321 cgactatctg gaagggaaaa acctacctga acaggtcgcc agaacaatag agaactggta
     4381 tctgaaaacc gaacataaac gccgtccgcc aattaacgtt ttcgatgatt tctggaaaaa
     4441 gtaataattt gcacctattg gattgtgcga aaaagagtaa tttgttcacg ccggatgcgg
     4501 cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca atacattgca ggagtcgaat
     4561 aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc gcctcttttt
     4621 taatcgcctc ccgcctgtta cactggatag ataaccagca ttcggagtca acagtggtac
     4681 ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa tatcccgaac
     4741 atttacattc attccttaat gacttaccca cccgaccagg ggtgtatctg tttcatggcg
     4801 aaagtgacac catgccgctc tatatcggca aaagcgttaa tatccgcagt cgggtgcttt
     4861 ctcatttacg caccccggat gaagccgcca tgctacggca atcccggcga attagctgga
     4921 tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc aaagaacaac
     4981 agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgtgccctg caattaaatg
     5041 aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc cccaacctgt
     5101 ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc gctgatgaac
     5161 aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt gcatgttttc
     5221 gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat gaggaacatg
     5281 cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt gtgttggcct tggcaaggtg
     5341 cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt caaaactggc
     5401 tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg acacccgccg
     5461 ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt aactatgaaa
     5521 ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga acaacagttt
     5581 accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcacg cgccctggtc
     5641 ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta atcacaaaca ccaccccgcc
     5701 gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct tacagacatc
     5761 caggcgcgtc ataatattga aatccatcga catctgccct gccgtcagtt tcgcttttac
     5821 cacctggtcc gccgtaaagg caaaaggctg taacggtttt aaggtatggt taaagcggtc
     5881 tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt ccatgccggg
     5941 aaacaaagaa aactccccat tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc
     6001 acgttttgcc ggtgggaaag tggaaatttc gcgcgtttca ccggcagcgt ttcgccacag
     6061 attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc cgttcttttc
     6121 attgttatcg ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt tacgttagtg
     6181 gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg atcctgtcca
     6241 ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaagatt
     6301 attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa
     6361 aattagcatt aaattgcttt ttctgttccg gcgtcaggat gttgtaaatt ttgttctggg
     6421 tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg atctgcgctt
     6481 cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg cggcgttctt
     6541 ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgtgg atctgctgtt
     6601 tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga ccgaacttgc
     6661 ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat
     6721 gggccaggtt agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac
     6781 gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg
     6841 ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg
     6901 aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt
     6961 gatgagagaa tattacaaaa cgatgatttt gcagagatta tgaagaacta taccggatga
     7021 ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact
     7081 taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc
     7141 cgcgcgtaat cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt
     7201 tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa
     7261 attcagcgtg tcacttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga
     7321 aaccacccga taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag
     7381 cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa
     7441 cgctttgcca agttccaacg tacaggccag cgcgccaaaa tgtctgacgc tgaaatgggt
     7501 aaacgtacca ccaggctcct gatggaacac cagcgcctcc agccccgccg cacccagcca
     7561 cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg
     7621 caaggagcca cgaattgcgg tatgtagatc aaggtgccaa cgcacagatt ctttgccctg
     7681 gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc
     7741 agcaaatagc tgccaa
//
LOCUS       DAJTRQ010000160         7589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000160 DAJTRQ010000000
VERSION     DAJTRQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            89..2269
                     /locus_tag="OO332_003672"
     CDS             89..2269
                     /locus_tag="OO332_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5943986.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2410..3693)
                     /locus_tag="OO332_003673"
     CDS             complement(2410..3693)
                     /locus_tag="OO332_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5943987.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3828..4898)
                     /locus_tag="OO332_003674"
     CDS             complement(3828..4898)
                     /locus_tag="OO332_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5943988.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(4876..5094)
                     /locus_tag="OO332_003675"
     CDS             complement(4876..5094)
                     /locus_tag="OO332_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5943989.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5179..5802)
                     /locus_tag="OO332_003676"
     CDS             complement(5179..5802)
                     /locus_tag="OO332_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5943990.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6124..6831)
                     /locus_tag="OO332_003677"
     CDS             complement(6124..6831)
                     /locus_tag="OO332_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5943991.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(6779..7213)
                     /locus_tag="OO332_003678"
     CDS             complement(6779..7213)
                     /locus_tag="OO332_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5943992.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7286..>7589)
                     /locus_tag="OO332_003679"
     CDS             complement(7286..>7589)
                     /locus_tag="OO332_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5943993.1"
                     /translation="ANAEFIAAFNPNVALALLDERERNLQYIKSRDQENEEIALTVGK
                     LRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTVKEVGDA"
BASE COUNT         1758 a         1892 c         1857 g         2082 t
ORIGIN      
        1 tcgccttcct gcaactcgaa ttatttagag tatatccatt tattatcttt ctgcgcactt
       61 cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag gcgtgtttct
      121 cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta aaaaagaaga
      181 agcaaaaaaa ggcactattg cctggtctat tctctcttct cataatacgt ccggaaatat
      241 ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct ttgttggtat
      301 tgtacagacc gctaaagcgt ccggaatgga acgtttcccg ctgccgtatg tgctgaccaa
      361 ctgccataac tcactctgcg ccgtcggcgg caccattaac ggtgatgacc atgtttttgg
      421 tttatcggca gctcagcgtt atggcggtat ttttgtgcct ccgcatattg cggtcatcca
      481 tcaatatatg cgtgagatga tggctggcgg gggcaaaatg atcctcgggt cagacagcca
      541 cacccgttac ggtgcattag ggacaatggc agtcggtgag ggtggcggtg agttggtaaa
      601 acagctgctt aatgacacct gggatatcga ctatccgggc gtggttgcgg tgcatctgac
      661 cggaaaacct gcgccgtatg tggggccaca ggatgtggcg ctggctatca ttggcgcggt
      721 gttcaaaaac ggttacgtca aaaacaaagt catggagttc gttggaccgg gcgttagcgc
      781 gctctctacc gatttccgta acagcgttga cgtgatgacc acggaaacca cctgtttaag
      841 ctccgtctgg caaactgatg aagaagtgca taactggctg gcgctgcacg gtcgcggcca
      901 ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca tcagcgttga
      961 tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg tgtatgaaat
     1021 cgacacactg aaccagaacc tgaccgacat tctgcgtgag attgaaattg agtccgaacg
     1081 cgtggcgcac ggtaaagcca aactctcgct gctggataaa gtggaaaatg gtcgcctgaa
     1141 agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg tcatcgcggc
     1201 ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg cggtttaccc
     1261 gtcatcacag ccggtgttta tggatctagc caaaaaaggt gtggtagcag atttgattgg
     1321 cgcaggcgca atcatcagaa ccgcattctg tggcccatgc tttggcgcgg gcgatacgcc
     1381 aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc gcgaaggctc
     1441 taagccagcc aatgggcaga tgtcagcggt ggcgctgatg gacgctcgtt ctatcgctgc
     1501 gactgcggca aacggtggct atttaacctc tgccagcgaa cttgattgct gggacaacgt
     1561 gccggagtac gccttcgatg taacgccgta taaaaaccgt gtttatcagg gctttgtgaa
     1621 aggggcaact cagcaaccgc tgatttacgg accgaacatt aaagactggc cggaattggg
     1681 tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg aagtcaccac
     1741 caccgacgaa ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc cgattggtct
     1801 ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc attaagcaga ttgctggtca
     1861 ggagcatatt gatccgctgc aaactgaaat tggcagcatg gtctatgcgg tgaaaccagg
     1921 cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt gtgattggcg gtctggcgaa
     1981 tattgccgag gagtacgcga ctaaacgcta ccgttctaac gtcatcaact gggggatgtt
     2041 accgctgcag atggcggaag tgccaacctt tgaagtgggg gattacattt acatccctgg
     2101 cattaaagcg gcgctggata atccgggtac gacgtttaaa ggttatgtga tccatgaaga
     2161 tgcgccggta acggaaatta cgctttacat ggaaagcctg acggctgaag agcgcgagat
     2221 tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat cagatgtaaa aagcgccatg
     2281 tgaatgtagg tcgcattcgg cacttattgt cggatgcgat gctggcgcat cttatccgac
     2341 ctacaaatcg catcgaaccg taggccggat aaggcgttta cgccgcatcc ggcaaatagt
     2401 taatagctct tacttcttcg cctctgcaac cactttgctg cccacgccgc ggttattgta
     2461 ttcccacatg cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt cgttatcatc
     2521 tacgctgccg gtattacccg caaacggacg attagagatc accgcatcgg cccacggttt
     2581 agccgtgtta aaaccttcgt tgatggcgct atcacggatc accacctgac cgttggtatt
     2641 agcatcaaca tccagcgagc ggcccagttg cgccacgcca tcaccggaag cattgaaacg
     2701 gctgtttacg gcgaggaaac cgtaatagat gttagacagc gtagccggtg caaacacata
     2761 tgcttcttgc tgggtacgtg agttcaccac gcggaattcg gtgttatcga acaccactgc
     2821 gccgcgacca gaaacgatat ccacatcccc ttcaatatag ctgttggtca ccagcgtacg
     2881 cggctgacgg ttggtttcca gacggttctg cacaccgctg ttggtgacaa agaaggtgtt
     2941 ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg tcaccgtcag tacgcagtgc
     3001 taccgccgga tggttacctg catctacact atcgcccagc gtgttttcga tggtcagatt
     3061 ttgcagttgc agtccattgt tttgtgacca gaagaccgca gaacacagaa caccgatact
     3121 gtcgctgcgt ttgctctggc agctatcgta catataccac gctggtttac ctggcatata
     3181 tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg ctcatgcccc catcaaggga
     3241 aagcccaatc ttcacatcaa tcggtttttc acctgtaccg tacagagtga ttccacctgg
     3301 agcagcaggg acatacaccg ttccctgata ctcaccaggc atcacggcaa tatactggcg
     3361 cttgttggtg cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg tatgcgttac
     3421 accttgagta cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg ttatcgggga
     3481 cggattccac gctgcagcac ctggtgtcag ggatgcaaaa tagtgttgcg catcgaaatt
     3541 cagcgcttct tttgccgaca gaatcgggcg agaagaggta ccaggcgcag tttgatcaga
     3601 aggacgttga tcgggtggtg ttgagctaca ggcggtcagc gtcacgccaa aagccaatgc
     3661 cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc tccgggctat gaaatagaaa
     3721 aatgaatccg ttgaagcctg cttttttata ctaagttggc attataaaaa agcattgctt
     3781 atcaatttgt tgcaacgaac aggtcattat cagtcaaaat aaaatcatta tttgatttca
     3841 attttgtccc actccctgcc tctgtcatca cgatactgtg atgccatggt gtccgacttg
     3901 tgcccgagaa gatgttgagc aaacttatcg cttatctgct tctcatagag tcttgcagac
     3961 aaactgcgca actcgtgaaa ggtaggtgga tccccttcga aggaaagacc tgatgctttt
     4021 cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa gcggttcacg acgagtagat
     4081 gcaattatgg tttctccgcc aagaatctct ttgcatttat caagtgtttc cttcattgat
     4141 atcccgagag catcaacatg caatgttgta gggatggcaa tttttacgcc tgttttgctt
     4201 tgctcgacat aaagatatcc atctacgata tcagaccact tcatttcgca taaatcacca
     4261 actcgttgcc cggtaacaac agccagttcc attgcaagtc tgagccaaca tggtgatgat
     4321 tctgctgctt gataaatttt caggtattcg tcagccgtaa gtcttgatct ccttacctct
     4381 gattttgctg cgcgagtggc agcgaccggg tttgttgtta tatggccttc agctattgcc
     4441 tctcggaatg catcgctcag tgttgatctg attaacttgg ctgatgccgc cttgccctcg
     4501 tctatgtatc cattgagcat tgccgcaatt tcttttgtgg tgatgtcttc aagtggagca
     4561 tcaggcagcc ccctccttat tgctttaatt ttgctcatgt aatttatgag tgtcttctgc
     4621 ttgattcctc tgctggcgag gattttttcg tagcgatcaa gccatgaatg taacgtaaca
     4681 gaattatcac tgttgattct cgctgtcaga ggcttgtgtt tgtgtcctga aaataactca
     4741 atgttggcct gtatagcttc agtgattgcg attcgcctgt ctcggcctaa tccaaactct
     4801 ttacccgtcc ttgggtccct gtagcagtaa tatccattgt ttcttatata aaggttaggg
     4861 ggtaaatccc ggcgctcatg acttcgcctt cttcccattt ctgatcctct tcaaaaggct
     4921 acctgttact ggtcgattta agtcaacctt taccgctgat tcgtggaaca aatactctct
     4981 tccatcctta accggaggag ggaatatcct gcactcgcgt acccatcgac gaactgtttc
     5041 aaggctcctt gggcgtcgct ggcgtgcgtt ccactcctga agtgtcaagt acatcgcaaa
     5101 gtctccgcaa ttactcgcaa gggagcgagt attggtttat tttctccaac aaaaaaggag
     5161 ccgaagctcc tttgatgatt aaaattcgaa ttgtctcgcc cgaaggcttt tcagcattgg
     5221 ttttgcccgt tcgaaaaggg cgcttgcttg gtcaagtcgt gtcgcctccc ttagcagtac
     5281 atctctgttt ttcgtcaccg tgtaaaaagt ttcaaacgcg atgtcataca gcttgctcgt
     5341 gtatgatgag ttcagttcct tcaatatcgg gtacagacgt ttgcagatgt cctgcgcatt
     5401 ctccatctgt acctgcatat agcaaaggag gatgatttct tcgtctgtga attgctgcgc
     5461 tggttgcatg ctgcgaagtt tcttttcgca ctcgatgaag tatcggcgta tctggcggcc
     5521 tttttcgtta cgctcgacca tcgccgtttc tttggctgta tcgagggtga ggtgatagtc
     5581 tttgctacgg cgatcgcctc cacgtccttt gatccccctg ttttggtgaa ccaaaatgta
     5641 atcgacatca acggcaaagc cgtattctgt tatgcgattt ttaatccatg tagagaagtc
     5701 acgacgtact tccaaaaatt cgtgtaaatc acgggcatta acgagaagag ttgtttcgtt
     5761 ggatatagtg ccgttgaata cggggatgag ttgactggtc atttttatgt cctttgcatt
     5821 tgttcagata gccctgtgtt cagcagggcg gtcaggtact tgaacaccgt gcaaagtcgg
     5881 cccgtatcct taacctttcg gctgtttttc ggtatacgcg ctacccgacc atatctgaaa
     5941 aatggacata aaaaatccgc atgactgacg ggtgcggttt ccgctttgca aggtgtgttc
     6001 agcaccatga agcggaatat agccccgtta atgcggtgtt gtcaaatcat gtaggcctta
     6061 tcttgctgta agccgcgcca ttcgggcttt tccccacatt tggggaaaac tatcttgtca
     6121 aaatcatgca gcctttcgct ctccttcgat gagttcctct attttgtgca ctccagcctc
     6181 gttgtaccga aatgtttcaa cctgcttgtc attgtgcgcc gatttgtcga tgaaccactt
     6241 cccgtacttt tctgttttga gcatgtaggt gttggctatg cgtcctacct tgttggctga
     6301 aataccgtag cgctggccga cttccgtagc agagtagtag tgctctgaga tagctggcag
     6361 aggtacaaca tcgcgaccaa caatcgggtt gaacactcca gctactgctg cctgctttga
     6421 ttctgaggac agattcggga acatattcac cagtcgatcc gccacgttca aagctatttc
     6481 catggctcgc gcacggtcta ggctactttc tggttgcggt actggttgag gatggttggc
     6541 aaccttttcg cgctgattga agtagccgtc ctccagtttt tcgaacacgt cccatgcctg
     6601 atccgtttcg agcatcttcg cgtgacgggc ggctccgcgc tctgtccaga ggatgaggtg
     6661 gcgtgcattt ggggcacttt tcacaaagta gtttaaagct actttgtgtt ttagcgcgcg
     6721 cagctcgtca ccaacgacct tgtagaagtg taccccctca acaaagcgcg acttgttttc
     6781 atgatggttt tgccggatgc gcacaacatc cgttccgtac aactgcgcca atgtttggtg
     6841 ggtagttgtt tttaaagtca gctgctaaca catagcattg tcgctctccc atttctggca
     6901 ttcgctcact acagcttatc caggcatccg gagttaccgg cactggctgg gcggtataaa
     6961 gcggtgttat atctgcccga aaattacatg ctttatgcag ccgcacccac cgttcgactt
     7021 ctgctttgtc agaatacata ccagtgaacg tgttatattc acggtcaatt tgcgtgaagg
     7081 ttaccttcca cgccaccggc tctgctgtca gcgatgccag agcaatttca taagcacggc
     7141 gttcaacatt gtctcgcaca tccaaactgc tgatacgctc tttgatttct ttaatcagtt
     7201 ctttgtcgtt cacttatgcc tccgattccc ggatgtcacc caaatcaacg ctacaatgcc
     7261 aaccagtgca acgagcgcaa aactatcaag catctccaac ctctttaacg gtaactgtct
     7321 gtgcctccag ttctgctatg cgcttttctg aggcttcaag taacgcctgc ttatcgcgta
     7381 gcgcttcttc cagttcagca acatggcatt cactatcaat aaggttgttc tctgctgctt
     7441 ccagctcaac acgcaacttc cctaccgtta gcgcaatttc ctcgttctcc tgatcgcggc
     7501 ttttgatgta ttgcaggttt ctttcccgtt catccagtag tgccagcgca acatttggat
     7561 taaaagcagc aataaattca gcgtttgca
//
LOCUS       DAJTRQ010000161         7508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000161 DAJTRQ010000000
VERSION     DAJTRQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="OO332_003680"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="OO332_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR5943994.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1158..2117
                     /gene="rbsC"
                     /locus_tag="OO332_003681"
     CDS             1158..2117
                     /gene="rbsC"
                     /locus_tag="OO332_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5943995.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2142..3019
                     /gene="rbsB"
                     /locus_tag="OO332_003682"
                     /pseudo
     CDS             2142..3019
                     /gene="rbsB"
                     /locus_tag="OO332_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3145..4074
                     /gene="rbsK"
                     /locus_tag="OO332_003683"
     CDS             3145..4074
                     /gene="rbsK"
                     /locus_tag="OO332_003683"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5943996.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4078..5070
                     /gene="rbsR"
                     /locus_tag="OO332_003684"
     CDS             4078..5070
                     /gene="rbsR"
                     /locus_tag="OO332_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5943997.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5036..6463)
                     /gene="mdtD"
                     /locus_tag="OO332_003685"
     CDS             complement(5036..6463)
                     /gene="mdtD"
                     /locus_tag="OO332_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5943998.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6486..7178)
                     /locus_tag="OO332_003686"
     CDS             complement(6486..7178)
                     /locus_tag="OO332_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5943999.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1909 a         1816 c         1979 g         1804 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
     1861 ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg
     1921 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
     1981 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
     2041 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
     2101 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
     2161 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
     2221 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
     2281 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
     2341 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
     2401 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
     2461 acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
     2521 catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
     2581 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
     2641 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga
     2701 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
     2761 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa
     2821 atcggatgtg atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga
     2881 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga
     2941 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
     3001 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
     3061 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
     3121 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
     3181 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
     3241 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg
     3301 cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc
     3361 gttcgccagc tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc
     3421 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
     3481 catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt
     3541 gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
     3601 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
     3661 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
     3721 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
     3781 catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
     3841 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct
     3901 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
     3961 gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa
     4021 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
     4081 gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt
     4141 atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa
     4201 gaacttaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
     4261 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
     4321 gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa
     4381 cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta
     4441 ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
     4501 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
     4561 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc
     4621 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg
     4681 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
     4741 tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc
     4801 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
     4861 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
     4921 atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt
     4981 gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact
     5041 ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac
     5101 catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
     5161 tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc
     5221 gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca
     5281 gaacattgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
     5341 tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
     5401 atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg
     5461 tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
     5521 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat
     5581 aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
     5641 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
     5701 aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
     5761 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
     5821 ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc
     5881 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac
     5941 ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
     6001 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg
     6061 gaagaagttc attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca
     6121 ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa
     6181 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg
     6241 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
     6301 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
     6361 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga
     6421 tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg
     6481 ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
     6541 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
     6601 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
     6661 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc
     6721 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
     6781 tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga
     6841 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
     6901 caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca
     6961 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
     7021 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg
     7081 ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg
     7141 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt
     7201 tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat
     7261 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaattatttt
     7321 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
     7381 acggcaaaca cgccgccggg tcagcgggat tctcctgaga attccgtcag agaaagcaaa
     7441 aataaatgct tgactctgta gcgggaaagc gtattatgca cacctcgcgc cgctgagaaa
     7501 aagcgaag
//
LOCUS       DAJTRQ010000162         7461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000162 DAJTRQ010000000
VERSION     DAJTRQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            15..1073
                     /locus_tag="OO332_003687"
     CDS             15..1073
                     /locus_tag="OO332_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5944000.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1116..1619
                     /gene="yeaK"
                     /locus_tag="OO332_003688"
     CDS             1116..1619
                     /gene="yeaK"
                     /locus_tag="OO332_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5944001.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1620..1724)
                     /locus_tag="OO332_003689"
     CDS             complement(1620..1724)
                     /locus_tag="OO332_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944002.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1894..2340
                     /locus_tag="OO332_003690"
     CDS             1894..2340
                     /locus_tag="OO332_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5944003.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2297..3118)
                     /gene="nimR"
                     /locus_tag="OO332_003691"
     CDS             complement(2297..3118)
                     /gene="nimR"
                     /locus_tag="OO332_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5944004.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3215..4396
                     /gene="nimT"
                     /locus_tag="OO332_003692"
                     /pseudo
     CDS             3215..4396
                     /gene="nimT"
                     /locus_tag="OO332_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="OO332_003693"
     CDS             4451..4798
                     /locus_tag="OO332_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5944005.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="OO332_003694"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="OO332_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5944006.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="OO332_003695"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="OO332_003695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5944007.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /locus_tag="OO332_003696"
     CDS             6315..6413
                     /locus_tag="OO332_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5944008.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="OO332_003697"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="OO332_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5944009.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6542..6973)
                     /locus_tag="OO332_003698"
     CDS             complement(6542..6973)
                     /locus_tag="OO332_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944010.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6967..7194)
                     /locus_tag="OO332_003699"
     CDS             complement(6967..7194)
                     /locus_tag="OO332_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5944011.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1832 a         1791 c         1801 g         2037 t
ORIGIN      
        1 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
       61 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      121 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      181 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatag
      241 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      301 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      361 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      421 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      481 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      541 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      601 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      661 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      721 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      781 tccggatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
      841 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
      901 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
      961 atgacaacct cggtcatcag gagggggatt tagcgattac gttacatgat gcgtataaag
     1021 cttccgatga gcgtttatat gtcaataagc agaacaaaaa cagccgttca tgataacctt
     1081 ctgtggttgt ttgcttgtaa tctcaggagc gtgaaatgac tgaaatggct aaaggaagcg
     1141 tgacgcatca gcagttaatc gctttattat cacaagaagg tgctaacttc cgcgtcgtta
     1201 tccatgaagc ggtaggtaaa tgtgaagcgg tgtcagaaat tcgtggcacc gctctaggcc
     1261 agggtgctaa ggcactggta tgtaaagtca aaggcaatgg cgtaaatcaa catgttctgg
     1321 cgatcctcgc agccgatcaa caggccgatc tgagccaact cgccagccat ataggcggat
     1381 tacgcgcttc gttggccagc ccggcggaag ttgatgaact cacaggctgt gtcttcggcg
     1441 cgatcccccc tttcagcttc catccaaaac tcaaactggt tgccgaccct ctactcttcg
     1501 aacgatttga tgaaatcgcc ttcaatgcag ggatgctgga taaatccgtt attttgaaaa
     1561 ccgcggatta tttgcgcatt gcgcaaccag aactggtcaa tttccgccgc actgcgtagc
     1621 tagcctatcc gttcaataag cagaacggac aaaaccagca caaccgcaat agcaaaaaac
     1681 gatgatgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg cccccgggag
     1741 atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac agttcgcctg
     1801 attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaag cgtaaagtaa
     1861 gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt gctgatcctg
     1921 ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt ttcaattctg
     1981 gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat tgaaaaacag
     2041 gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat cgccagcggg
     2101 acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct ggtggcaatt
     2161 gcagtaggcg ttattgtctc ctggctgggt gggcgcggcg tgacgttaat gggcagccag
     2221 ccgcaactgg ttgctgggct gctggtgggc actgttttag gcgtagcgct gtttcgcggc
     2281 gtaccggttg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg gaaacagtag
     2341 ttaatcccgc aatatatcgg cctggcgttt gccctaggcc tttcttaaac atagtaataa
     2401 aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta tcgcctttca
     2461 ctaatccctg aagcgccata atcagctgta actgctgacg ccactggcga aaactcaacc
     2521 ccgtctcttt cactatcagt cgggcaagat tgcgttcact catcgcaaaa aagccagccc
     2581 attgccccaa tgacccccac tcgacgggcc ctttcgccat catctccacc atcgtgcgga
     2641 ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga gggagttcat
     2701 caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc gtcgtcctgt
     2761 ttgccagggt taaaattaat tcccggcata agggcgatat tttcaatgtg caacatgttg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctagcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgctttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc gggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcaac atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg
     6601 gctgaagcat ttcgtctgca gtctgaaccg caggcaacca tttttcagca agtctgcgct
     6661 gggaatcgct ggctttatcc catttgctcc acagtgcctc acgatcgtaa tcgtgcgctt
     6721 ccagcgtggg gcgggcgatt tcaaaatacc cctggcttgt ttctatttgc tcagatttta
     6781 ataacaactc aacacgtgct gccgctggta gaactgatat atgaaattcc aaagcctttg
     6841 aacccgtacg cttgcgacgt agttctggat gattggctgc aagcttttca agattgcggc
     6901 gaatactcac ctcatgttta ggaaagcatg acattcctac gcattcttgc gctgttaccc
     6961 aatgggttaa catgatgttt tctccatata ccgagaaggc caaatctggg ctggttccat
     7021 atccaaatat ctagcaatga ttgcttcgta tcgtggtata tggcagcgaa atacgttacg
     7081 catagtgtca ggctttaagc ctgcctctct ggataattga gatagtgtct tccccttcat
     7141 acgaacagca gcctttatct gctctgcgtg ccagtcttgg ttttttgctg acattctgcg
     7201 atccttactc tcttaacgtt ttcggtaact gataccggct accgctatcg ggaaaaacga
     7261 tatcgcagaa aaatgcgcat ttcaagagct atgcacattt ttattttaac caatgcaaat
     7321 caaagaattc tgtaaaattc tttatataac agagagattg agcatgacag gtgaaaaaga
     7381 aagcaaaaat gcagagcgca tttttatgcc ccggaaataa tgcgtttcaa ggaacgcctt
     7441 gaagaggcaa tgaatggaga a
//
LOCUS       DAJTRQ010000163         7432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000163 DAJTRQ010000000
VERSION     DAJTRQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.165"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            214..290
                     /locus_tag="OO332_003700"
     tRNA            214..290
                     /locus_tag="OO332_003700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Arg,seq:tct)
     gene            complement(336..1097)
                     /gene="envY"
                     /locus_tag="OO332_003701"
     CDS             complement(336..1097)
                     /gene="envY"
                     /locus_tag="OO332_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5944012.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1280..2170)
                     /locus_tag="OO332_003702"
     CDS             complement(1280..2170)
                     /locus_tag="OO332_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5944013.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="OO332_003703"
     CDS             complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="OO332_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR5944014.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="OO332_003704"
     CDS             complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="OO332_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5944015.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1729 a         1934 c         1947 g         1822 t
ORIGIN      
        1 ttccgttagg gcggtaaatt gtactttgat acatgaaaat acgggttttc ttgattcaga
       61 cgcgcagcgg tgtgcgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg
      121 tcgttcactt gttcagcaac cagaccaaaa gccattgact cagcaagggt tgaccgtata
      181 attcacgcga ttacaccgca ttgcggtatc aacgcgccct tagctcagtt ggatagagca
      241 acgaccttct aagtcgtggg ccgcaggttc gaatcctgca gggcgcgcca tttcttcttc
      301 atttatgccc gtcttatccg tttccgcttt gcccttcacc acatcacttt ttgtcgctgt
      361 ttggcgagat aattcaacgg tgtcaggctg taaaacgcct taaaaacaga gataaagtac
      421 gacgtgctgc tatagccaca taattgcgcc acctgagtga tatttttgtt atccatcaat
      481 aacatctgta cggcgtaacg catacgacac tctgtgacaa tctggctata gctggtattt
      541 tcgtttttta atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt
      601 cgcagattcc agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg
      661 ctgctgcgta agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac
      721 aatacggtaa aaagtaatgc gcgcgtcaac tccgcttcgc agggtttgct gggttgctgc
      781 ttgctgtggt tggcggctag ccggaacact tccggtgtac ggctatgaca agcgataacc
      841 ggggttgcca gccgcgacca gggctttaca caggttaatt tcttatttaa aaacaacaga
      901 taatagttga tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt
      961 tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta
     1021 gcatggttat tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca
     1081 ctgctgctca attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat
     1141 gcttcctgca tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcatgctt
     1201 tttagcaatt aatcttgatg gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc
     1261 cgacttacct ggaggggatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca
     1321 gagagaaata gagcatgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt
     1381 ctgcgtccgg tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aacagttcat
     1441 aaacaatgcc gcttgctggg gcggagcttt ggcaatcggc gctggcctgt agataacgtt
     1501 cacgctgttc agcggtaagt ttatccacgc cgctgccatc ctgtacccag acattaactc
     1561 cggttgcttt aacttgttcc agcagttggc gatagccatc gggcgacatg tttccggcga
     1621 aaaaactact gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt
     1681 ttaaccatgt tagcaaaggt tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt
     1741 ccgcgctgat gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt
     1801 gctggagatc ggcagccagc aggcgattaa gatagctttc cagcgctgca gacgactgtt
     1861 tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac aatcagcttc aggccagtct
     1921 gttgcgcagc tgcggcacgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat
     1981 cgccgtaacg ggtccattgc aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca
     2041 tcagcccctg ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa
     2101 tacctttcat cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga
     2161 acttacgcat ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac
     2221 gttgattaat cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc
     2281 aggcgtcgta gtgcgtctcg ccggtccaaa tattccagcg gaccccgaca ccgccaagct
     2341 gcgcgccctg agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa
     2401 tagtctgtcc gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct
     2461 gaatatcctg gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg
     2521 aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc
     2581 gcagcatggt atcggatgcg ccattttgcc cgttcagcgg caactgctgt tcgacggcga
     2641 ggaaaaagat ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt
     2701 tcaccggcat catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga
     2761 gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt
     2821 agctacgata gcttttccca ggcgctgcac cgccgacatt attgttagcg gtactcattg
     2881 cgccggaacg caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct
     2941 catgcaaacg gcggaaattg aagcgttgct gattttgctc tggggtcagt ggggttatca
     3001 gcgcctgatt atcaatgtca tcaatcacca gccgggcgta gtgctgcgtc gcaggcatgt
     3061 catccagacg ctggttcacg taggccagtt gtcggatcag tgccggatcg tccggaagcc
     3121 ctttatgcgc ctgttcgagc atttcccgcg actgtgcgat atcaccgcta tcccacaagg
     3181 cgtaaccaag cgctgcctgg gtgttgctat tattcggttc cagttccagt tccagcgcgg
     3241 cgcgcaaatc actcaccgcg gccggaacat tatgacgttg gcgataaatt gtcgcccgcg
     3301 caacgtaagc gttggcagaa ggggcaatat tgattgagcg cgtgagatcg ttcagtgcga
     3361 gttctggctg agctgggata taacgttgcg catgcagcca ccagtattga gcattgtttc
     3421 ccagctcacg ttgttctgcc tgttgaagcc agcgatcgcg agccgcgcca tttcccgctg
     3481 cctgagcggt gttggcggcg gcgagcagat cctcattgct catgtcgtga agactgattt
     3541 tctgccaggc cgccagtgcg gtggcgtagt cctcaacctg atacgcctga taggctaccg
     3601 cacgatgttg ccaggcgctc ggttgtcgtt gttcggcctg aagccatgca tacaacgcca
     3661 caccgggtag cgtgtcccga taacactttg ccagacggtt ccaggcggca gcatcgtagg
     3721 aaggcgacat atcgcccagc aagcgaacta ttgccgggca attatctgca atacccggca
     3781 actgactttg ccactgacgt tgctccgcca gcggtaaggg tttcgataaa atcgccacct
     3841 tcgccggcgt tgctaggtaa ggatgacttt ccagcagaga cgccagtcgc gccattaaag
     3901 tctggctgac acgcgcatcg ccctggaaag gatagcgttg cagcaataaa tcggcagctt
     3961 cgcgtgactg ctcgttctgc atcagttgcc aggtcagttg atccaggcgg ggaagatttg
     4021 tcggttcttg ctgatacagc aatcgtgcca gacgcagaga ttcagccttg ttacgggtcg
     4081 ccacgctgac agcataacgc tcctcaagca tttcattggc ggggagggtg gcgagcagtt
     4141 tttgcgctgc gtcgtactga ccctctttta acagcaccgg tagcgtcgcg ccaacaacat
     4201 actggcggtt atcggcaaac tgcaccgtat aattcgccaa tgcctgaacg gggttggcgc
     4261 tgtatttaga taacagatag agccaacttt tctcttgtgc gcccgtggta aacagcggct
     4321 tattttcaat gagataatgc tggaggcgtg ctttttcgcc acgataagcc agcgcggtcg
     4381 cgtaagtaat atatgactga ggatcggtga agatcccctg tgattgcagt gccaggatcc
     4441 gatcgtccag ctgcccggca agaagcacgt caaaccactg acggcgttct gccgcgctta
     4501 atgtgttctg ctggcgtgct tcattgtata gcgtatctgc ctgggaccat tgtttcaggt
     4561 agattgcccg ttgcagcaga tcggttcgca gcgtttttcc ttccggcgat gcagcaaacg
     4621 tcgcatcgtt cagttgcgct ctggcgacag gtaattgtgc cagccgcagg gcattctgcc
     4681 cgacttcact gcgacaacgc agggtcggcg cagcgtcgcc cgctttttgc tgggcaagca
     4741 gttcttcaac agtcgtaacg cttttcactt caactggaat agccgccaga ctgcgctcaa
     4801 gtcgggcatc tcctgggtga cgtttcagtt gatcctcaag caacagccgc gcccggtcat
     4861 catgaccaaa atgacgatag gcttccgcaa ggtataaagt cagcggaata ttatccggca
     4921 cctgttggtg tatatattca aattcgcgga tggcggttgc ttcgtcgtta tttttctgtg
     4981 ccttcagcgc cttatcgaga cggggataaa taacaaaatg gcgataatcg ctcagcccca
     5041 gctcttctgc gctggtgccg atattgtctg cgagtgcgct ggtactcaat aaagacgtca
     5101 gcagtaaacc agaccatccg atgacgcgat taaggttatt ctccttcatt ttcggactcc
     5161 agttgcgcaa cctgttctgt gtttaaacct gctttgagta atagtgattg catcgaaact
     5221 tgtaattcgc gttgaattgt caggacgcga tccaacgttt cctggctgat aacgccttcg
     5281 gtgaccaaaa acttgccgag cggcagagaa ctgcgttcat ggcgcaataa caacacgtta
     5341 attgctgaac gattaatatg accgagcgtg gtcagtattt cggcgaacag gaactgatgc
     5401 ggcacatatt gccgccagat ttcaccggtc tgctgttccg tgagccactg atgctgaacc
     5461 gcattgtaca acattgcccg cggatcgtga ccgcgtcggc gtgcatacca gtggcgtaac
     5521 cctgtgacaa tttgtccccg cagaacaatg acgtaacgca ctttgcgtcc gactttacgc
     5581 gtcagggccg ccagcgaaac cgggtcaata ccatcttcac tgccgacaat taactcatca
     5641 ttttccagac gcagcggcag tactgcataa tgcagcgcta cggaggctgg catttcggca
     5701 atcagcgagg acgggatctg ccaggcatcg atggattccc acgccacgcc gttttgctct
     5761 gcctgcgcct gtgccagctg ctcggcgcta atcagcccct gcatcagcat tgaaccgccc
     5821 aggcgtagac cttcgacgcg attacgcagt gctgtatcga gttgttcttc agtgatgacc
     5881 tgattttcca gcagaatttg acctaacggg cgcaacgagc gggtatcgcc agtgacgctg
     5941 gggaagtcat gcgttgtttt atcccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgcca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc tgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agccgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt at
//
LOCUS       DAJTRQ010000164         7294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000164 DAJTRQ010000000
VERSION     DAJTRQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.166"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            94..1205
                     /gene="yneK"
                     /locus_tag="OO332_003705"
                     /pseudo
     CDS             94..1205
                     /gene="yneK"
                     /locus_tag="OO332_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1355..2545
                     /gene="ydeA"
                     /locus_tag="OO332_003706"
     CDS             1355..2545
                     /gene="ydeA"
                     /locus_tag="OO332_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5944016.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OO332_003707"
     CDS             complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OO332_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5944017.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3447..3881
                     /gene="marR"
                     /locus_tag="OO332_003708"
     CDS             3447..3881
                     /gene="marR"
                     /locus_tag="OO332_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5944018.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3901..4306
                     /gene="marA"
                     /locus_tag="OO332_003709"
                     /pseudo
     CDS             3901..4306
                     /gene="marA"
                     /locus_tag="OO332_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4338..4556
                     /gene="marB"
                     /locus_tag="OO332_003710"
     CDS             4338..4556
                     /gene="marB"
                     /locus_tag="OO332_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5944019.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OO332_003711"
     CDS             complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OO332_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5944020.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5750..6868
                     /gene="ydeE"
                     /locus_tag="OO332_003712"
     CDS             5750..6868
                     /gene="ydeE"
                     /locus_tag="OO332_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5944021.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6995..7090
                     /gene="mgtS"
                     /locus_tag="OO332_003713"
     CDS             6995..7090
                     /gene="mgtS"
                     /locus_tag="OO332_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5944022.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1905 a         1775 c         1659 g         1955 t
ORIGIN      
        1 acagcgccac gcatcacctg cctttgatag ttcttgcagc tcaaatgccc tatatagtga
       61 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
      121 tacatatctc ttcctgatga ttttcccgct cgtaatattg cacctcaagt aaaagaagtt
      181 ttaaaggatt ttattgatgc actcagtacc ataatatgtg atgaagagtg gcgcacgtct
      241 ttaaacacca actcagccac aaaaaagata tttaacaacc ttgataactt atcatatatt
      301 cagaggactt cttttcgggg taacgacaca ctatacaatg aaaaggttca gtttaaactc
      361 acttatcccg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat
      421 ttaagcccta tttagataat tttcgccatg atggagaaat taatattttt tgcgctccca
      481 acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc
      541 tgtttatgaa tgattttatt gaacaatttc caatgatcaa ccttggcgcc cccatcaaaa
      601 gagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct gcagattact
      661 tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc
      721 cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga
      781 tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga
      841 tggattcatt aggttctaac aaacatgtac aacccgaagt ctgcatctgc ttcttatccc
      901 ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt
      961 tattgcacta tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc
     1021 cagcatcatt aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactat
     1081 ttgtaaaaaa tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc
     1141 gctcccctgt cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc
     1201 attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca gagaaacgga
     1261 aaacgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt
     1321 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
     1381 agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga
     1441 atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca
     1501 ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat
     1561 gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat
     1621 tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat
     1681 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
     1741 ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc
     1801 gatggtctta ggtttaccgc tcgggcgcat tgtggggcag tatttcggct ggcgaatgac
     1861 cttcttcgcg attggtattg gggcgcttgt tacccttttg tgcctgatta agttacttcc
     1921 cttactgcct agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
     1981 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
     2041 cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc
     2101 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
     2161 taatcaatat gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg tgtgtctggc
     2221 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
     2281 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
     2341 tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg
     2401 tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg
     2461 cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc gctggccagt
     2521 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg
     2581 ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga
     2641 accccatcag gcgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc
     2701 cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
     2761 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa
     2821 taatcatcgc tatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaggcaa
     2881 tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa
     2941 tcgctttctg ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt aagccgcctg
     3001 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccagcgtaat
     3061 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
     3121 cggcactgtt catgttgcct gccaggccaa gaaatagcgc aacagttgtt aatgggttag
     3181 ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg
     3241 gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag
     3301 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
     3361 gtggcatcgg tcaattcatt catttgactt atacttgcct aggcaatatt atcccctgca
     3421 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
     3481 ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt
     3541 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
     3601 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga
     3661 cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg
     3721 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc
     3781 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
     3841 ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg
     3901 atgtccagac gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac
     3961 aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatga
     4021 gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa ccggtcattc
     4081 attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc tgaaggaaag
     4141 taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac aaactctgac
     4201 ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga ccaatatgca
     4261 gggcgaatcg cgctttttac atccattaaa tcattacaac agctagttga taacgtgaca
     4321 acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg cttattctct
     4381 tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct tgtgccaatg
     4441 tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac atgggtactg
     4501 gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct atgtagtttg
     4561 ttctggcccc gacatctcgg ggcttattaa cttcccacct ttaccgcttt acgccaccgc
     4621 aagccaaata cattgatata cagcccggtc ataatgagca ccgcacctaa aaattgcaga
     4681 cccgttaagc gttcatccaa caatagtgcc gcacttgcca gtccgactac gggcaccagt
     4741 aacgataacg gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc ccagatccca
     4801 taaccaacaa ttgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca
     4861 atagtaacca gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag
     4921 aagggaatga ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt
     4981 gagtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc
     5041 agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac
     5101 accagtacgc caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc
     5161 ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa
     5221 gccagtccag caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa
     5281 ctgatggtta atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa
     5341 aagatggccg gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg
     5401 ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc
     5461 gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa
     5521 tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg
     5581 cattaatgcg caaataagtc gctatacttc ggatttttgc catgcgtccg atggttaata
     5641 acaacgacga ggcaagaagg gcgctggtag agcgtcgtag ggataagttc atttgtctgt
     5701 ccggcagtgc gtttcgttat gttttatttt agagatgtaa agaaatagca tggctacgct
     5761 gccatttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta
     5821 tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc
     5881 ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt
     5941 tattgccatt cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa
     6001 ctgcgcctat tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc
     6061 caccagcaaa acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat
     6121 cggtccgccg ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc
     6181 agctatctgt tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa
     6241 aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc
     6301 actgttgtgg tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc
     6361 atgcatttca caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc
     6421 ggttgttctt ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg
     6481 acttaaccca gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg
     6541 actggtcggt tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt
     6601 atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc
     6661 gccgccagga atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc
     6721 gattaaccca ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt
     6781 catcttagcg ttggtgatca ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag
     6841 accgtggggg cagcccgcgc tttgttgatt taagtcgaac acaataaaaa tttaattcag
     6901 ccttcgttta ggttatctct gctaatatct ttctcattga gatgaaaatt aaggtaagcg
     6961 aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat
     7021 ggccgtactg ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg
     7081 ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac agttgtgtac
     7141 aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt
     7201 gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg
     7261 atcgttgaca cagtagcatc agttttctca atga
//
LOCUS       DAJTRQ010000165         7086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000165 DAJTRQ010000000
VERSION     DAJTRQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..607
                     /gene="hchA"
                     /locus_tag="OO332_003714"
     CDS             <1..607
                     /gene="hchA"
                     /locus_tag="OO332_003714"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5944023.1"
                     /translation="YHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSL
                     FRNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISL
                     CHGPAAFLALRHSDNPLNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNI
                     INDDITGRVHKDRKRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(715..2073)
                     /gene="hprS"
                     /locus_tag="OO332_003715"
     CDS             complement(715..2073)
                     /gene="hprS"
                     /locus_tag="OO332_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5944024.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2073..2744)
                     /gene="hprR"
                     /locus_tag="OO332_003716"
     CDS             complement(2073..2744)
                     /gene="hprR"
                     /locus_tag="OO332_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5944025.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2877..3290
                     /gene="hiuH"
                     /locus_tag="OO332_003717"
     CDS             2877..3290
                     /gene="hiuH"
                     /locus_tag="OO332_003717"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5944026.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3399..4403
                     /gene="msrP"
                     /locus_tag="OO332_003718"
                     /pseudo
     CDS             3399..4403
                     /gene="msrP"
                     /locus_tag="OO332_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4404..5038
                     /gene="msrQ"
                     /locus_tag="OO332_003719"
                     /pseudo
     CDS             4404..5038
                     /gene="msrQ"
                     /locus_tag="OO332_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5296..5946
                     /gene="zinT"
                     /locus_tag="OO332_003720"
     CDS             5296..5946
                     /gene="zinT"
                     /locus_tag="OO332_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5944027.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7024..>7086)
                     /locus_tag="OO332_003721"
     CDS             complement(7024..>7086)
                     /locus_tag="OO332_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944028.1"
                     /translation="NRVDTSTAPDIEWPEEPDTM"
BASE COUNT         1990 a         1606 c         1486 g         2004 t
ORIGIN      
        1 gtatcatctc catgctgcag gtttcgaatt cgaagtggcg accatttccg gtctgatgac
       61 caagtttgaa tactgggcta tgccgcacaa agatgaaaaa gtaatgccat tctttgaaca
      121 gcataaatcg ttattccgta atccgaagaa actcgcggat gttgtcgcca gcctcaacgc
      181 tgatagcgaa tatgcagcaa tctttgttcc tggtgggcat ggcgcactta ttggtttacc
      241 agaaagccag gacgtggctg ccgctttaca gtgggcaatc aaaaatgacc gttttgttat
      301 ctccctttgc cacggtccgg cggcttttct ggcacttcgc cacagcgata acccactgaa
      361 tggttattct atttgcgcat tcccagacgc cgcagacaaa caaacgccag atattggcta
      421 tatgccgggt catctcacct ggtacttcgg cgaagagttg aagaaaatgg gcatgaacat
      481 cattaatgac gacatcaccg ggcgagtaca taaggaccgt aaacgtctca ccggcgacag
      541 tccttttgca gcgaatgcgt tgggtaaact ggcggcgcag gaaatgctgg cagcttacgc
      601 gagttaatcc ctgttacatt gtgatgtaag cgcataatca atcgcagcca cgttaatact
      661 cacgacgtgg ctgcgttttt tcacggttaa cttatggcgt actgaagccc gatgttaatt
      721 tctttgcggt aacgtaattc ggaacacgtt atgcttactg agatagtgat acgtagcact
      781 tcctccatgt aattcggcaa tcgctttgac taaagaaagg ccaagtccct gacctacgga
      841 atgacgcgaa ttatctcccc gccaaaatct acggaagagt ttttcaggct cattaatttt
      901 cgttccagga ctggcgatat caatattaag agagccgttg gcatcaagaa aactggttat
      961 atgaatacgc gatttttctg gtgaatatcg aatggcgtta acaataagat tcgataacat
     1021 tcgttgcagt aaaattttat ccgcaaagat ttgttgattg cacttgacct taaagcaaat
     1081 ctctttctcg tctgaaagag attcaagata gtccaaaaga ttttcaactt ccttattgag
     1141 agaaagcgaa tccagtttca ccaaaacatt gtttttgtct gccctggcaa gaaacagtat
     1201 attctctgtt aaccgcgaaa tattttccag ctcttcaatg tttccggcaa ttgttttttg
     1261 atattcagcg atacttctga tttgactgag cgtaacctga ttctgaccca gtaatgcatt
     1321 aattggcgtt ctaagttcat gagcgagatc gtcagcaaat tgactaaggc gctcaaaatc
     1381 tttgactaaa gcatgatgca ttttattcaa cgcctgccca agaggtttta gttctctcgg
     1441 taatgcgtta acctcaaccg gcactcggct atcgttataa ttcagcgctt ccgttacgcc
     1501 actcaacttt ttgatctctc gcaaccccgt cctgattaac aacggactta atactgagca
     1561 cagtataatg gcgacaatgc aaattataat gctattaatt ttatactgtt caagcatatt
     1621 atgtctggct gaagccaatt tagccacagt aaccgttaat gatggcgaaa cttcatcaat
     1681 gttaattcgt aaagcatcta tctctgtatc attaataatg cttctgtaaa tgcctgctgc
     1741 gctgattgtc tcactagcag gtatgttatt taacatgtca tcactgacat ttgtccggtt
     1801 aacaatttta ttgatgccat cactatgaat gatcaaaata tcctgactaa catccatcat
     1861 ccggttaaag tacacaggta acgtatctgg atttacccca tcaattaaca actgcttgat
     1921 ctgcgctgtc cggttaatga gtgttgtatc atcgcgccat ttcaactcac ttgccaggcc
     1981 attatagaga gtccatacaa ttccagcgcc agcaacagac tgcagaaata taaaaagcaa
     2041 ggttaaacgg acggttatag ataatctttt cattattttt ttaccgctac gaatgaatag
     2101 cccatccccc ggattgtggc gattagcttt tcaggaaaag gatcatcaac ttttgcgcgg
     2161 agcctgcgaa tggcgacatc caccgtattg gtatcactat caaagttgat tccccaaatt
     2221 tcactcgcaa taaccgttct gggtataatc tcgccagctc tggaggccag tagccaaagt
     2281 aactgaaact ccttgcgcgt cagtgtaata ctgatattgt ccctgctgac actttgactg
     2341 acagagtcca tttttaagcc actgatttct aatgttgaat tcagagcgtg atgttgcctt
     2401 aattgtgccc gaacccttgc taacaactca gaaaatgaaa aaggttttac cagataatca
     2461 tttgccccac tgtccaaccc tctgactctg tcatcgacag aatcccttgc agtaaggcaa
     2521 ataacagggg tttgctttgc cgttcttaac gtttgtaaga tctgccagcc atccataccc
     2581 ggaagcataa tatccagaat gatcaatgca taatcatcct tcagcgcaag ataaagccca
     2641 tctctgccat cagaaacggc atcaatgaca taacccgctt cggaaagccc ctgcgttacc
     2701 cattcctggg tcctttgatt atcttcaata agtagaatct tcatgaaaat aatatgccat
     2761 aaatcatatg ttatagaaac aacctggttc attacaaaat tgtaatgctg ctgtaaggtt
     2821 accctggacg ttttttcgct atcctcaaaa ctcattcaca tgacaaggat ataaacatgt
     2881 taaagcgtta tttagtactc tcggtagcaa cggcagcatt ttcattacct tctttggttt
     2941 atgccgcaca acaaaacatt cttagcgtgc acattttgaa ccagcaaaca ggaaaacccg
     3001 ctgccgacgt gacagtcact cttgaaaaga aagcggataa cggctggtta caacttaata
     3061 ccgccaaaac agataaggat ggacgaatta aggcactgtg gcccgagcaa actgcgacta
     3121 ctggcgatta ccgtgtcgta tttaaaaccg gggactattt caagaaacaa aatcttgaaa
     3181 gtttcttccc tgagattccc gttgaatttc atattaataa agtgaacgag cattatcatg
     3241 tgcctttgtt actcagccaa tatgggtatt caacctatcg tggcagttaa tgcgtaaata
     3301 tgtaaaagct gatctctgct actgttaggg aggtgactcc ctgacagatt aacagtaaac
     3361 agctcttgtt ggttaacgac aaaaaagtgt gatggcttat gaaaaagaat caatttttaa
     3421 aagaatcaga tgttacggcc gagtcggtat tctttatgaa gcgtcggcag gtgttaaaag
     3481 cactgggcat cagcgcagct gcattttctt tgcctcacgc tgcgcatgcc gatctgctta
     3541 gctggtttaa agggaacgat cgtccgcccg cccccgccgg aaaaccgctg gagttcagca
     3601 agcctgccgc ctagcaaaat aacctgccac tgacgccagc agataaagtc tccggttata
     3661 acaacttcta tgaattcggg ctggataaag acgatcccgc ggctaatgct ggtagcctga
     3721 aaaccgatcc atggacactg aaaatcagcg gcgaagtggc aaaaccattg accctcgatc
     3781 atgatgattt aacccgtcgc ttcccgctgg aagagcgtat ttatcgtatg cgctgcgtgg
     3841 aagcgtggtc gatggtggtg ccgtggattg gttttccgct acacaaattg ctggcgcttg
     3901 ccgaacccac cagcaatgcg aagtatgtcg ctttcgaaac gatttatgcg ccggaacaga
     3961 tgcctggcca gcaggaccgc tttatcggcg gcgggctgaa atatccttat gtcgaaggat
     4021 tgcgtctcga cgaagcaatg catccgctca cactgatgac cgtaggtgtt tatggcaagg
     4081 cgttaccacc gcaaaatggc gcgccggtgc gactgattgt gccgtggaaa tatggcttta
     4141 aagggattaa atcgatcgtc agtattaagc tgacccgcga gcgtccgcca accacctgga
     4201 atctggcagc gcctgacgaa tacggttttt acgccaacgt taatccgcat gttgatcacc
     4261 cgcgctggtc acaggctacc gaacgattta ttggttcagg cggcattctc gatgttcagc
     4321 gccagccaac actactgttt aatggttacg ccgaccaggt ggcatcgctg tatcgtggcc
     4381 tggatttgcg ggagaatttc taaatgcgtc tgacagcaaa acaggtgata tggctgaaag
     4441 tttgcctgca tcttgccgga ttgttgccgt ttctctggct ggtctgggcg atcaatcacg
     4501 gtggactggg tgccgatccg gtgaaagata ttcagcattt taccggtcgc actgcactga
     4561 aatttttgct tgcggccttg ttaatcacac ctctggcacg ctacgcaaaa cagccgttat
     4621 tgatacgcac tcgccgcctg ttaggcttat ggtgctttgc ctgggcgaca ctgcatttaa
     4681 ccagttacgc attgctggag ttgggcgtga ataatctggc gttattaggt aaggagttaa
     4741 ttacccggcc ttatttaacg ttaggaatta tcagctgggt tattttgctt gctttagcgt
     4801 tcacttcaac ccagtcgatg cagcgaaaac tcggcaaaca ctggcaacag ttgcataact
     4861 tcgtctatct tgtcgcgatc ctggccccaa tacattatct gtggtcagtg aagattattt
     4921 caccgcagcc actcatctac gctgggctgg ctgtactgct ttagccttac ggtataagaa
     4981 gttgctttcc cttttcaacc ggttacgcaa gcaggcccac aacaaattat ctctataact
     5041 tcatattgcc gacaaacttc gcgttatccc ggccggatgc ggcgcgagcg ccctatccgg
     5101 cccacacaga tcgcatcaag ttatgtttcg tttgtcatca acctttcatc accagaataa
     5161 tttattttat ttcaataaga tagttcaata cacctgagaa taccatgctc tcatttccta
     5221 atgagttgtt gcattttttc atatgttaca atataacatt acacataatg tacattaact
     5281 ctggaggaaa ttgttttggc gattcgtctt cataaactgg ctgttgcttt aggtgtcttt
     5341 attgttagcg ctcctgcctt ttcgcatggt catcattctc atggcaaacc cttaacagag
     5401 gtcgaacaaa aagctgctaa tggtgttttt gatgatacca atgtacaaaa ccgaacgctc
     5461 agtgactggg atggagtctg gcaatccgtt taccctttac tgcaaagtgg caaacttgat
     5521 cccgtctttc agaagaaagc ggatgcagat aaaaccaaaa catttgctga aattaaagat
     5581 tattatcgca aaggttatcc gacagatatc gaaatgatta gcattgagga cggcattgtc
     5641 gaattccata gaaataatga aacaacatcc tgtaaatatg attacgatgg atacaaaata
     5701 ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa agatcctgaa
     5761 agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg aaaatcatct
     5821 cattttcaca tttttatggg taatgattcc cagcaatctt tattgaatga aatggaaaac
     5881 tggccaacgt attatcccta tcagttgagt agcgaagaag tggtcgagga gatgatgtct
     5941 cattgatttt tttgcgctga cttcagttat ttaacagaac agtgcctggt agtaacaaca
     6001 ataatttacc aagacaaata agtaagacaa actcagacac gctcatactt gagtcgtgtc
     6061 ttttttatta tgtctgtaga aaaaaactga gaaaaaggcg aacaataaat acacaactca
     6121 gccatacaga ctggcagcgc aaatgtttcc aggagcaagt catctgtcat ggtaaagcta
     6181 accagaaata taggttaatt ttgttgttta aacctttttt acttgttagt ataatacaaa
     6241 ggctttcaaa aaagctggga aattcaacaa ctcttggaag ttatggactt tgtcccgcgg
     6301 ctttaatctg acggccgcgt tccttttttg gctcaattat tgaccccttc tctacgcaac
     6361 ttcagttccc gccagcaact ttgcggcagt ttcataggat caatgtctaa aagaataatg
     6421 gtgaccgata agaaaacgac tgaataactg tagattttcg ctcgaaacct tcctgtcagc
     6481 tccatagcga atcaagcgct gaatgtcaca gtatcgaaca gaaaacagtg acgatctaac
     6541 ccttcaagaa tattctacga ttgttctgtt taggaaaagc aaggcgggaa gtcgggagat
     6601 aagtcattga taaagtggcg gagagagggg gatttgaccc cccggtagag ttgcccctac
     6661 tccggttttc gagaccgatg atacagagtc gaaaaatcaa agagttaaaa atttttcttg
     6721 gaaaaaatgt cttaaaatcg tacacgaaaa atcaatgaat tacataacat taaagaagaa
     6781 tattccaagc cttcttgagt ccaccacctg ttttcaggtg tcattcagac gtgatttgcc
     6841 ccagattggg aaagcacgct tttttctcag cttattccgt tttgcctgtg tgttgaataa
     6901 ttccactgca gttatataaa atcggtaacg gctggaaatc attcaatact cgcactatcg
     6961 aaagtttacc agccaaccgc agcacgtcct gcatacgtcg tgtctgcggt ttttcttttt
     7021 cgcttacatt gtgtctggtt cttccggcca ctcaatatca ggtgcagttg atgtatcaac
     7081 acggtt
//
LOCUS       DAJTRQ010000166         6917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000166 DAJTRQ010000000
VERSION     DAJTRQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.168"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..223)
                     /locus_tag="OO332_003722"
     CDS             complement(<1..223)
                     /locus_tag="OO332_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR5944029.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAP"
     gene            743..1438
                     /gene="minC"
                     /locus_tag="OO332_003723"
     CDS             743..1438
                     /gene="minC"
                     /locus_tag="OO332_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5944030.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1462..2274
                     /gene="minD"
                     /locus_tag="OO332_003724"
     CDS             1462..2274
                     /gene="minD"
                     /locus_tag="OO332_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5944031.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2278..2544
                     /gene="minE"
                     /locus_tag="OO332_003725"
     CDS             2278..2544
                     /gene="minE"
                     /locus_tag="OO332_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5944032.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2916..3260)
                     /locus_tag="OO332_003726"
                     /pseudo
     CDS             complement(2916..3260)
                     /locus_tag="OO332_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3295..3414)
                     /locus_tag="OO332_003727"
     CDS             complement(3295..3414)
                     /locus_tag="OO332_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5944033.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3375..3560)
                     /locus_tag="OO332_003728"
     CDS             complement(3375..3560)
                     /locus_tag="OO332_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944034.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3661..3834
                     /locus_tag="OO332_003729"
     CDS             3661..3834
                     /locus_tag="OO332_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944035.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3836..4180
                     /locus_tag="OO332_003730"
     CDS             3836..4180
                     /locus_tag="OO332_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5944036.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4184..4519
                     /locus_tag="OO332_003731"
     CDS             4184..4519
                     /locus_tag="OO332_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5944037.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4576..6897)
                     /locus_tag="OO332_003732"
     CDS             complement(4576..6897)
                     /locus_tag="OO332_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5944038.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1871 a         1638 c         1571 g         1837 t
ORIGIN      
        1 taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg
       61 ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc
      121 acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag aacatcatcc
      181 ccacacctga caatccgata taaataatac gcatcatatt catagcacat cctgataatc
      241 caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc gctgacctgg
      301 acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta tgatagagca
      361 attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg caaaaaatgg
      421 aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg gatatttaaa
      481 caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa taagaagaat
      541 caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat gaatcagcgc
      601 catttatcac agaatagact tttactctga ataaatggga gggtgacttg cctcaatata
      661 atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa
      721 tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag gcagtagctt
      781 cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc aggcgctgaa
      841 agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac tcaacgtcag
      901 tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg caaccggttt
      961 gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaaaat
     1021 ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc ccgcaccgca
     1081 ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata ccccggtgcg
     1141 ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc acgttagcgc
     1201 tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc
     1261 gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc tgatggcgga
     1321 attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag aattttatgg
     1381 caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc
     1441 cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg taaagggggt
     1501 gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa gggaaagaaa
     1561 actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat gggttgcgaa
     1621 cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg
     1681 ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca aacacgcgat
     1741 aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa agcgatggat
     1801 tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt aatggcactc
     1861 tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt acgcgactct
     1921 gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg cgaagagcct
     1981 attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag aggtgacatg
     2041 ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag
     2101 gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga cattaacgcc
     2161 gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga acgtcctttc
     2221 cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg ataagttatg
     2281 gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc aaaagaacgg
     2341 ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta tctgccgcag
     2401 ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc tgagatggta
     2461 accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa cgtgacctta
     2521 ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt caaggcagag
     2581 tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc ggcgagtatt
     2641 cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag gctgttccca
     2701 cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta gtgtggatat
     2761 gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg atgaggtttc
     2821 ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat cctgtaaacg
     2881 aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt cacaccagcc
     2941 accgcgttcc acggggactc cacaccggca ccatggctat accccacccc gaaatgcccg
     3001 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
     3061 aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc gacaaattct
     3121 ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag acgcatgttg
     3181 taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga tttcgattcc
     3241 atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc aacatcagcc
     3301 cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat tgtgttcacc
     3361 gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag gcatttttta
     3421 cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata atactgtcgt
     3481 acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt aatcgttgct
     3541 gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc agattatctt
     3601 acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata ttgtcaatca
     3661 atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat tcctatcatc
     3721 acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt taagcagatc
     3781 ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat ctaagttgaa
     3841 gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc cagcgatggc
     3901 ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt tacttactgg
     3961 caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg gtgcagtaac
     4021 agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg ccgccgttgg
     4081 tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag gtgccgtgat
     4141 tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga tgaaaaaatt
     4201 tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg actacaatat
     4261 taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg aaatggcaaa
     4321 aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca gcgttgccag
     4381 ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag aaaaactgaa
     4441 tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg acaccatgcg
     4501 tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga cgcggttttt
     4561 tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg tgtcattgta
     4621 gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg gattcaggtt
     4681 gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt cgactttcac
     4741 accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt caacaaaagg
     4801 ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa aggtgcggaa
     4861 gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc cattggcttc
     4921 cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct ggaagatcca
     4981 cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt aacccgcttc
     5041 cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat cccctttcac
     5101 ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt ttttactgat
     5161 gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac tccctttcga
     5221 acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac cagccatcac
     5281 tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc cccctaactg
     5341 cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg
     5401 cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta caggatcgat
     5461 gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt tagcggctgc
     5521 caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg caacagtagg
     5581 cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt tagtcagata
     5641 ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt aggcaccggc
     5701 gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc tgataatctc
     5761 tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa ctcgggttgt
     5821 tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat catccaattc
     5881 agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg ttaatcttgt
     5941 tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat caaccgtgag
     6001 gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa gcgacagata
     6061 ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg aattagttgc
     6121 atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg atgatacctc
     6181 actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt catttgctga
     6241 ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta tggaagactc
     6301 cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc
     6361 gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt ctccctggtt
     6421 aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat gcgtggtgat
     6481 atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac cagccttgac
     6541 gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga tctccccttt
     6601 tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg cagtactttc
     6661 ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg caagaacgcc
     6721 gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa aagagggggt
     6781 aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc cactcgtttg
     6841 acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac cctgcaaaat
     6901 aacagcaccg ccaaatt
//
LOCUS       DAJTRQ010000167         6900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000167 DAJTRQ010000000
VERSION     DAJTRQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.169"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(703..2412)
                     /locus_tag="OO332_003733"
     CDS             complement(703..2412)
                     /locus_tag="OO332_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5944039.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2844..3563)
                     /locus_tag="OO332_003734"
     CDS             complement(2844..3563)
                     /locus_tag="OO332_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5944040.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3688..4244)
                     /locus_tag="OO332_003735"
                     /pseudo
     CDS             complement(3688..4244)
                     /locus_tag="OO332_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4228..4486
                     /locus_tag="OO332_003736"
                     /pseudo
     CDS             4228..4486
                     /locus_tag="OO332_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4595..5173)
                     /locus_tag="OO332_003737"
     CDS             complement(4595..5173)
                     /locus_tag="OO332_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944041.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5594..5831)
                     /locus_tag="OO332_003738"
                     /pseudo
     CDS             complement(5594..5831)
                     /locus_tag="OO332_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6018..6447)
                     /locus_tag="OO332_003739"
                     /pseudo
     CDS             complement(6018..6447)
                     /locus_tag="OO332_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6495..6680)
                     /locus_tag="OO332_003740"
     CDS             complement(6495..6680)
                     /locus_tag="OO332_003740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944042.1"
                     /translation="MDARNKKLVFWFVRVDDEGYPEIARCMEREFVTIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
BASE COUNT         1889 a         1590 c         1260 g         2161 t
ORIGIN      
        1 tttttctgct cattattggc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcagat aagcttgata caactggtct ggtatataca gtttctttga
      121 gtgcggttac gtccacagag gtaacggtaa aagtaacccc ttgtcgggac cactccgacc
      181 gcgcacagag gcattttcat cgctgacagg ccgcattgcc tcgcagggct ggaggaggat
      241 cggtgatatc cggacgaccg taccggctta cgccgggctt cggcggtttt actcctctgc
      301 catgtgcaac aacggagtgc cgccttacat gccgctggcg cggcatatcc tggtaacatt
      361 tttgttgata ccaccttccg gccttatcct gaccagtggg ctttcctggc atcgatgata
      421 ccgatgaacg cgcatgatat tgagccgacg atattacggg caactggaaa tactcactct
      481 ctggatgtga cctttatcca tgaagaagat ttggccacac catggaaacc tgaacaatca
      541 tcagtttacg cccatgtaaa cccctacggg atatttgaac tcgatatgga aacaaggttg
      601 ccaatcgagg tggttgcctg aaagttggca gttacccggt cactacacct tgttggagtg
      661 aacatatcaa aaacaggtaa ttacacaatt taaattacag tctcataaaa aatgattaaa
      721 tctatttatt atttcaataa acgttttttt catcttcttt gaaaattgtt ctgctatagt
      781 atccggttcc agattgttct cttttattat tctacaatac tctaatattg tttctttgtc
      841 tctatttact acagatacaa atacaccagg aataccatct ttattagttg cggacagcat
      901 ttcgaccaat cgtcttgtgg taaaactata gttttttaga atattacaaa aggcacgtat
      961 tgtttcggta tggccattat aaaatgccat aaaaagtcct gatgtaccgt tgggattttt
     1021 tatccataat aattcatcta tatattcctg gtgagggggg agtattttta gtatatctcc
     1081 atacgccata atggcctttg tgtgcccatt ttgaagtgcc ataaacaacc catgctttac
     1141 tttcaataat tctttaattt gttcactact aagcattttc ttattaagca gacttccgaa
     1201 catacgaata ctatctgcat gtccattctg caatgcaaaa aataacccag gtaaatcatg
     1261 gctgtctttc gcagctaata gatataatct cttatcggaa gttaacgggg ttttaaagac
     1321 tatttcccca taggctttta ttgtgtctac atgtccgttc ataaaagaca tgcataaacc
     1381 cggcacattg tttgccccta ccgcagcaag caatgtaagt attgtttctt gactaagatt
     1441 gcacatattt aatatcttac catatacttg aataatgtct gaatatccgt tttgaagtgc
     1501 tatataaaat gcaggagtac catcagagct tctcgtggcc agaaggttca ccagtttaga
     1561 ttgagaaatt tcaccatttt tgtatttttc catcagtata aataatactt ctgtcagatt
     1621 ctttgttatc gcatgatata taactctgga attgatcaat tcatctggta aaagtttcac
     1681 tgtgtttgtt agaggggaat tatgcctatc ttcaaatatt acctgcccgg gttgctccag
     1741 accataaatt ttccaataaa aatcatcaaa aaaatcctta gcggataatt gactcagttc
     1801 gaaatggcta tcacaagtaa atacagtacg tttatgagta acagttcgat taggatcgaa
     1861 aaactgaaca acccattttt cactttcttg tacgcgcttt attttcaacc ctaacgccat
     1921 ttggtggttt aatgtgcatg ctgataatgt tctgacaaag tctccatttt ttttcatgtt
     1981 ataaaaaaaa ttagtaataa ctgttcccca ctttttattt tccacaaaat aaatataagc
     2041 tggtgatttt gaaaaatctt ccgttttttc caaacataca ttttttgcaa tagcatcagg
     2101 ggtgctaaga agctgatagt ttaccttccc atttgtttcc ataaaattca tttcccaata
     2161 ggaagctagg tgacggcaaa ctatttgtct tccatatata tcttttgcca ctccatttat
     2221 atttaccttc acatttctct tttctgaaaa ataaggtgct gttttattct taaactgaat
     2281 tagttcactg gagtttgcga ccctcataat agaacttgtt attgttctat tatctctgtt
     2341 aacagacata tctgttgata acgaattttt tgttgaaaat atactagatg aaaatgtttt
     2401 atttaacggc atagaagaca accatagaga tgttataata ctattagatt gcagttacta
     2461 atagtaaaag cctccttaca aggaggtagc ttattcagaa tgctcctgta agatagcgag
     2521 ctcgtcaacg atacagatga tctgattata ttgtgcaaaa ggagaacagg aaaataggca
     2581 ctggaaaaga tgaaggcctg atgggagcat taactaatgc aaaatccgac aggttaatat
     2641 cccgaaaagc aatcaaacga ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt
     2701 cggatttcag atgagtagct cagcagttta tgagccgaat gctctgtcac gggcaggaat
     2761 caggctgcat acgcagtgcc aaatctaatc gtagttattc taaccagttt tcaagctaca
     2821 aggtggtgta gctggcatct tctctactct atcatcaaac gataaaatgg ttcctctcgt
     2881 aacatttgct cttgcctttc acttccatct cgatcgcttc gtagttcatt acgatagctg
     2941 acatatttcc agtcttcagg tctaacatct gacgccggat actccccggg agcgattttg
     3001 cttcttaata gattagactc gagacacatt ataaattgtc gtattttatg aagtaataaa
     3061 gcactatatt gcgagcattc ctgatctgat tttacatata gcgtaaactg agctcctatc
     3121 cccacacgag attgctgaga aactcgattc atatcagtta tcttccattt atctatagga
     3181 ctgtcctctg aaaataataa accagacaaa atttggaatg ccagaggaac ttgctcccta
     3241 gctatactaa tatgaaactt gtccccaaca aaatccccct tactctgagg attttcacga
     3301 catgcatgaa taaacacatc tccactatgg ttcacaatct ggaatccttg gtacatgaca
     3361 gaaaaaaatt gacatatatc agagtaagtt ggcacgttcg atgctacttt gaaatagtac
     3421 gctgggtatt gccgtatttg gttgtacaat atattgaagt tatttttcaa tctttcattc
     3481 gcagaaagca tctgcggtat ttcagacctc acaacattca aagaatctaa atttagtttt
     3541 agacagggct tttttatggg catagaaaac gtcctctata aaatagatat attatcttat
     3601 attgattgtt tatcatcttt tataaaacga tacattatat gtaatgtatg taagcgtaag
     3661 ggtcttctcc gatcatgatg ggaaggctta gaacgccata ttcagtttcc catagtggaa
     3721 catcactccc agcttaaagc gctcccggtt gcggtagccc ttggctttta ttctcagcag
     3781 cctgatcttg ctgttcagtg cttccgcatt tccgtttgat acgccatggc gcatggcgtt
     3841 caggataccg tacaaccttt tccctatcgt tttagccaca ttcttcatca tggggacgtc
     3901 actgttggcc gcagccatct ctgccagtcg ttgcgtcttt cctcgctcca tggtctgctc
     3961 cagatatcct ttgccagttc tttcagcgcc cagcacaggc tcgtcagctt catctgcgca
     4021 cgcagccata tcagcttttc ctgcctggat tcggtcatcc atttatcgct gtgctgccac
     4081 aggaagcgag ttccctttgt ctgacgacga ctttcaatag gaagatgcgg atgttcattc
     4141 tggcgggttt tatccaccac ctcacccggt tgtttcgcca catgaaagcg atcgaaggct
     4201 attttctcaa ccgcattggg taagtggatg cgcgctgctc ttagtttgct gagccagttt
     4261 agcattgcct tctacatgac cgatgcctga gccggtatac cgagctttat tgtcctcaac
     4321 cagtcatgtt ataagcaaga aatgcactca gcggatagag cgacataatc tgaacctgcg
     4381 gacacatctt aagcggctta cccgcaaaac catatgcttc tcaaagtctg atgatatgca
     4441 ctacaagatc atcggctggt atctgacaat caaccaccac cactaaatct gcgtcacgac
     4501 ctgcaactga gtctggttga acaggctgag gccctgctga agcaacgcga acctctcgac
     4561 tcttgttgga ttttaccaaa tagtttttct aaaattaaac acatttatat aagttctttg
     4621 ctgaacaaaa cgcagcccgc catccttttt cattcataaa ttcagcaatg gcagataaaa
     4681 ctttttcatc aagcatataa aaaataatgc cagtagactc aatatgcctc atacaaataa
     4741 ggccgcacat atccgtaata tatttgtcaa tgctgctacc atttgcatgg taatatgcca
     4801 tgctattatc tgatgatttt acatatgcag tatgacatat ttttccttca cgattaacaa
     4861 aactgacaaa accactttca ccaggagtaa tatataaaga tgaatttaac gtgcgctcac
     4921 taatattcat taattgttga aattcaatgg aatagttttc agttaatgta tctattatct
     4981 tggctttgtc tttctttgtt atctcattgt ctttttcggc aagaaaaatt gccactttat
     5041 gacatgcatt cagctcgtta attttacctg ataaaactgc ttttcttacc acatcgttga
     5101 actcatcgat agtgtgtttt ttattatata tatcatgaaa aatatagcct aaagacttaa
     5161 acgagacttt cattttatat tctccttaaa tattaacagg atatatatat cacattttcc
     5221 tctatatttt cagaaaccag ctatcatttt ccccattact ggtacgtcgc ttcagatatt
     5281 tccgtagcaa gcgataaccc tgcgcactat gaggatgcca ttcattataa tgtttgaaca
     5341 ccttcgcgag actactttac tgctgttaac acgtcaggtt cgaacataac actgatttaa
     5401 tcatatttca tcgttttgac gaagttttct gccattccat tttgcaccgc cgtgtgtttg
     5461 ggggcaaggc cgaccatctg tgtttcattc gccagatata agtgatgaga tggcattctc
     5521 cctccttcac ggcagaagca ttttgaccgt ttaggggatg cggacgattt tcatgcctgc
     5581 taagatactt tcgttactga tgctgtccgt tttccaccat ttcatcatca ctgattggcg
     5641 ttgtgttttt ggtcagcact ccagctttac gtttctcttt taaccggtaa ctctctcctt
     5701 tgatattcag cgtgtagcag ccgatctagg atcgctgttg ccagcacgtg atctccgaac
     5761 atttctcccc agtctgcgac ccctttgttt gacgtcagta tgatgctcgc tttttcatat
     5821 cgacagttca tcgataggaa tttaaatccc caaaagatta aaaaagcacc gaagaatgcg
     5881 cgtttattca acacaacctg atcatcaagt aatcggtata gacacttaat attgcttaca
     5941 ttgattgatt caatgtatct ctgatggttt tttgctccgt tcccctcaac atggacgcaa
     6001 cttcccctat gcgactctca gccgcaccac cgtattcgag ccagcggctc atgcatcata
     6061 acctgctctg ccagacggta acccctcttc agccccgtaa accgcatctg acctccgcgc
     6121 agctcgcact tcagcgggtc aaccttcagt cactgctgat acatccctct ccttgtgatt
     6181 tgcctcgccg tttttctcac tgtctccgtt atggcgtaca ccacatcttc cagtaatcgc
     6241 cgcttcaccg gactcaggac cccgtatcac catccggaac cccttatccg ccacatgtca
     6301 ggagaacctt tccataaact cgtcgccact catcacaagg ttttcttctc gttttgtccg
     6361 gtggctgttg taacgcaggc caatttcatc ctgaccggca taatgttcca ggcggctcat
     6421 ctgcaccggc tgcttctcca gccagttaag ccatcggttt ccctgctgaa aaatatcggc
     6481 aaaacgggga agcatcagaa aggcggaata acgccatccg gccagtgaac cgtaccgcat
     6541 tcagggcaat acatgccgtc ggcgttgata ccggcaggaa ttgtcacaaa ttcccgctcc
     6601 atgcagcggg caatttccgg atacccttcg tcatcaacac gtacaaacca gaagaccagt
     6661 tttttgtttc tggcatccac aaagaacgga atattcaggt ctgcgcaaca ctcaacggca
     6721 tcgtcaaaat tatcaaagcg cagaacctca gcttcttcgt aaccggctca tttaaaccgt
     6781 ctggtctgtt tcctccggct ccacaaaaat aatgtccatc attttgaagt ggacactatc
     6841 gtatggaata ccggacctgg atcactgaag ctttacgcct tcatttcgaa gaatatttac
//
LOCUS       DAJTRQ010000168         6898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000168 DAJTRQ010000000
VERSION     DAJTRQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..508)
                     /locus_tag="OO332_003741"
                     /pseudo
     CDS             complement(1..508)
                     /locus_tag="OO332_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            651..773
                     /locus_tag="OO332_003742"
                     /pseudo
     CDS             651..773
                     /locus_tag="OO332_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1035..1601)
                     /gene="ipgB2"
                     /locus_tag="OO332_003743"
     CDS             complement(1035..1601)
                     /gene="ipgB2"
                     /locus_tag="OO332_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5944043.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1883..2197)
                     /locus_tag="OO332_003744"
     CDS             complement(1883..2197)
                     /locus_tag="OO332_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944044.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2275..2385)
                     /locus_tag="OO332_003745"
                     /pseudo
     CDS             complement(2275..2385)
                     /locus_tag="OO332_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2709..3386)
                     /gene="ospD1"
                     /locus_tag="OO332_003746"
     CDS             complement(2709..3386)
                     /gene="ospD1"
                     /locus_tag="OO332_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5944045.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3757..3894)
                     /locus_tag="OO332_003747"
                     /pseudo
     CDS             complement(3757..3894)
                     /locus_tag="OO332_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3976..4274)
                     /locus_tag="OO332_003748"
                     /pseudo
     CDS             complement(3976..4274)
                     /locus_tag="OO332_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4863..5022)
                     /locus_tag="OO332_003749"
                     /pseudo
     CDS             complement(4863..5022)
                     /locus_tag="OO332_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5026..6255)
                     /locus_tag="OO332_003750"
                     /pseudo
     CDS             complement(5026..6255)
                     /locus_tag="OO332_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6275..6622)
                     /gene="tnpB"
                     /locus_tag="OO332_003751"
     CDS             complement(6275..6622)
                     /gene="tnpB"
                     /locus_tag="OO332_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5944046.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6622..>6898)
                     /locus_tag="OO332_003752"
     CDS             complement(6622..>6898)
                     /locus_tag="OO332_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944047.1"
                     /translation="GACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLPVT
                     LTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIREIKGSSH"
BASE COUNT         1755 a         1615 c         1497 g         2031 t
ORIGIN      
        1 accaactgtg ccgtcgccag cgattgtaat cacattcgat ataattaaac actgttgccc
       61 gcattatttc ccggctgata aagtgttctc cgtggagaca ttccactttc agcgaatgaa
      121 agaagctttc cacgcaggca ttatcgtagc agcagccctt tgcgctcata ctgccacgca
      181 gattatgcca cttcagcagc gcctgataat ctgctgaaca gtactgactt cctcggtccg
      241 agtgaacgat aacgttccgg ggtctcttac gccgccacag cgccatctgc agggcatcgc
      301 aggccagttg cgccgtcagg cgtggtgaca tggaccagcc aataacggca cgtgaccaca
      361 ggtcaatgac cacagccaga tacagccagc cttcatctgt acgtaagtac gtgatgtctc
      421 ccgcccactt ctggttcgga ccactggcgt aaaaatcctg ctccagcaga ttttctgaca
      481 caggcaggcc atgtgcgcgg taactgaccg tattgctggg taagatcaat ttgctcactg
      541 gtatttacta cgtgcatcga atgagtgatc aagagccgta tgacgcgaga gtgtcacgta
      601 cggttccgta agcagccgga gagtgaaact ctctccggct gactcgactc gcaggttccg
      661 acagcggtga cgaacatgca gcggtgttgt tctcgctgat cggcacatgc cgtctgaaca
      721 atgtggagcc agaaaaatgg ctgcgttacg ttattgaaca tatccaggac tgaccggcaa
      781 accgggtacg cgatctgttg ccttggaaag ttgatctgac ttctcagtaa atatcaatat
      841 gattctgacg agccgcttac ctttgagcag agaaagggga gagggatccc acatccatga
      901 gctaggaaaa tacaaaaact ggctatacaa caattcaata tattaggact tacttgcgag
      961 gagaaatata gcaccgctca ggcaattcct gggtatttac ttttcttagt ttctttactc
     1021 gggatgttct gtcatcagaa aggcgattct aaatttgtaa tatagtcact catattccca
     1081 cgtaatttct ttagatcaat atattgcaca tttttctcaa aaaactgatc ggaaataaga
     1141 tttgcaacat tgcttgttgt atcattttta acgcttctat tcatcccttc acaaagttcg
     1201 tccattttct ttttgaaata actgcttcct gatacaagat gatttatgga actttgtgca
     1261 gcattgatgt ctagagaaat gcgataatct ttctcaacct gtttaaatat ctgtttacgg
     1321 agattatcgc cgatttttcg gctgtgttct gcacaaaact tatcaataca ttggttcacc
     1381 acccgggata cgtagacttt tttttcatca tttttccaat ctgtaatatt ttcaccgatt
     1441 tgttctgaaa cagagctcaa aatactagta tttattttgg ttgatgatat ttttactatc
     1501 cttttcccca ttgtacagcg gataatctca tccacctttc ctgaaaaata tcttttgtga
     1561 gataaactga ttccaaaatt attaaaagat gttccaagca ttataatcac tttgtcatgt
     1621 aaaatagtaa cattgtcatt attttagctg attcaaacaa ttctgtaaat gctttgagtt
     1681 ctttactccc ttggctcaat gatggaccgt ctggaatccc agagactaaa ctctctgaga
     1741 aagagagtat gaaaaaactt ggggcagtcc ctaaatgaag ggaccaacag cggtaaaata
     1801 accggcatca ttcataatga tgccctgtta gttgtagtta tgaagattta cgtcattaaa
     1861 attgctgttc gtagtgtcag tcctatggtc tggcatcagc tgaggatcgt agccgataca
     1921 tcactggctt cacgacattc gtgttgaggc tattcatgaa aactccacgc tgaaaacgcc
     1981 gttttgtata aacggtcacg gtatgcccgg agccactgtt gctgatgaat tcgataaaac
     2041 cttggcgttt cttgaagcta tcgttaatgc ggataatgaa acaacgattg gtgagattcg
     2101 ctcttttgcc gacgccctgg acgctgtccg gttcaatcgc aacaaaatca accggcagct
     2161 gagcaaaccc aaccttgcct ctctggcgct ggagcatgaa gtgatttggc tgggacgtag
     2221 ccgctgacag cttctataaa agtatgatcc tacccacgta atatgaacac aggcttaagt
     2281 gaggttctgg ttttcaaatt gttcaggact gagacctcca caccaactgt gccgtcgtca
     2341 gcgattgtaa tcgcacttga tataattaaa cactgtatat ttcatttgtg gagtctccag
     2401 tctgaggggg ctttatgcat tccgtattgg gcactatgat gccagaaatc tgcaaggctc
     2461 cacaccttcc tgtatttccc ttcctctatc acagtgcact ttacttaata gggtgggatg
     2521 tgttcattac atttattgtg ttttttatga ttgtttttgc gtccagtgtc gttcgtcatg
     2581 ggaaactggt tctatgggta gtatcgctca gtctagttaa ctgtttgttt ctttttagaa
     2641 attaagcaga tcacacaatt cggggagaga tccttctaag tgcaattctt tgcagctatt
     2701 tagcacactt aaaacaactc ttgtattttg tcagaaataa tattaaacat gtcttgttca
     2761 ttattgttaa gtctgtctgc tatttctgta agttttatcc cactatgatg gcatatattt
     2821 atatatgcca tcatagcctc gatattccct ttccctgcgg ccagaaataa tcctggtaac
     2881 ccctcattat ctcttacatc caatagttta atcgtttctt ttggtgataa atcaaaagtt
     2941 ttaataaaat ctccataagc ttgaatcgca gcggaatgac cttcttgaag agctatgaac
     3001 aaagcagatt ccccagcatt atttcgtgct gcaagaactt ctagtttttc ctctttgggt
     3061 atttcggctg ccaacagcga ttcaccgtat ttacgcgctg catcggcatt tccatgccaa
     3121 atcaatgaaa acaaattatg gcggttatac tccttattat tctttgtcgg aatggatatg
     3181 ctctgtattt tggccaattt tctgaaagaa acaccactat atcccccccc tgatttttct
     3241 tcattgatgg cgtgggatat agcggtattt gtcacgttaa tgatattatt tttcattcct
     3301 ttattttcat tagcagtatt gctgcctatt attaggtgca tatttttgtt gtttgctgga
     3361 tgtaatccat agttatttat tgacattttt gttcatcctt tttgctaaaa aaaacaataa
     3421 cctatttttg taatgtaata gatattagtc tattgctaat ctaaaaaaac cattgccaca
     3481 ttgaatggct atattcacca ataaattctt tcgatttcgt atattgacat atcgtagata
     3541 tatcttatcc ctaagattca ataatcgagt ccgtttacca ttctgtgcaa gcgccttcac
     3601 tcagaagtga ccgtcaaggc gatcaccaaa cttgataata aagcagctca ttgccatacg
     3661 ccagtcctta aggggcgttg tccatttctg tgatgctgcc tagatttaac tgccagctcc
     3721 ggcttatcta catctaaata tgtgagccta ataccgctac actttgtcca gactatgttt
     3781 ttctccggga agtgggctaa ccgtctccat aaagtgacga acttttttca acagtggcca
     3841 tattgaacgg cactgatgat tacgggttat tgtctcatga agagccggcc acatcactga
     3901 cagcagtgac ttcaggcctt cagcaccaga cagcctgaac cagttaatcc atcgcccgac
     3961 agatttagta atctagggct ggtttttctt cagctgttgc ggttcgaaag tgccattacg
     4021 atcacgcaga gtacggattt ccagaggttc atctccagta gtgataatct tcgttaagca
     4081 accattgcgg gaattgctgc cagatctaag ctgatttttc tcgcacccaa gatgatgagt
     4141 catattagca ttgagtacgg tttcattttc agcatccaat caaactgact gagatcttca
     4201 ggatttttga gacttttggc tagttcatta gctaaagccc gcaacttatt agatgtcaac
     4261 tgtttttcat tcataaattc accttcattt gatatcaaat taaatagttc aacatcagag
     4321 aattatacaa acttatgtac aggctcctat gatcttcgaa tcctgaagat tatagtgaaa
     4381 ataagttttc acgttgaaat cccacatagt tagtatatga taaataatca tggatgacct
     4441 gattaacctc ttggggtagt gcttttatag aagaataaat attctactaa tttaatacat
     4501 cacctaaaat agtattgttt tttatggttg gttgaaggtc gtggtgatga tgttagtcct
     4561 tttgagaata ttccagtgcc agatcttact tataataaag gggcttggca gtttggataa
     4621 actattgttc ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt
     4681 tcatactgaa atccccggaa gtattcaatc aaacaattac accataaaag gaagaggggg
     4741 ccctaattaa gttgtcaagc atgtttatga tacctgcagg gtataaattt tctggctgca
     4801 agcagacaca tataaaacgt ctttattgat cctacccaca taatatggac acagtcgtaa
     4861 gtgaggctct ggttttcaaa ttgttccgga ctgagaccac cacaccaacg gtgccgtcgc
     4921 cagcgattgt aatcgcactc gatataacta aacactgttg cccgcactat ttcccgatgg
     4981 ataatagtgt tctccgtgga gacattccat tttcagcgaa tgaattttca tctgttgctg
     5041 tatccagtca tacagtgact gcatcagtgg cgcggctctg gcttttcttg ccgccagacg
     5101 ctgttctgct gaacatcccc ggacttctgc ctcgatggca tacagttcac cgatacgctg
     5161 cagggcttcc gtggtgatgt cggttggcac tcttgcatgc acatcgtgga tttttctccg
     5221 gacatgagcc atacatgcgg cttccgttat tctgccggat tcgtacaacg cccggtaacc
     5281 accgtaagca tcggcctgaa gcactccgct gtaaccggcc agatgattct gtggatggat
     5341 acctttccgg tccggtgagt acgcgaacca gaccgccgga ggcatttccg aaccggcgtt
     5401 acggtcatca cgaacgtaga cccacagccg ggcagtcctt gttttaccgc tgcccggctc
     5461 ctggaccgga acggggatat catcggcatg gactttaccg ggcatcagca catactggcg
     5521 caggatgtca tacagcggtt ccagcagttc agcaacagca cctgtccagc gccccagtgt
     5581 ggcacggctc agctccacgc cctgacgacg gtatatttct gactggcggt ataacggcag
     5641 atagtctgca tattttccag cgacaacatg ggccagaagc cccgctccgg cataactgcg
     5701 tgcaatgggt tttgaaggca ccggtgcctg cacgatatgg tcacaccggc aacaggccag
     5761 tttcggacgt tgtgtttcga taaccttaaa ggcgctgctg ataagctcca gctgctctga
     5821 cacatcacat cccagagaac tgagttcgcc accacaggcc gggcagcatt cctcttccgg
     5881 acggatgacc cgggtttcac ggggaagtga ggcaggtaac ggtttacggg ctgaagactg
     5941 gcgcagggga gatggcagta tcgggtcata ttgctcaccc agcgtttccg ccatttcttc
     6001 ctgaagtgcg ctgattcgct cctgtgcttc cagtatctgc cgttcggttt ttgcgcgaag
     6061 tttttctgag cttttaccga actgcatgcg ctgcagtttc gcaaccagcg ccttcagccg
     6121 gttgatttcg gaagcataag ccgccacccg ctgtgagagc aggcggttgt atttcgccat
     6181 ctggcggatg gtgtcctgtt gcgtctgcaa cagtgcccgc aggcgggcat tctcatgaga
     6241 aagtgaggtg tccatatcct cactttacaa cgggttatat gcggatgcca gcgcgttccg
     6301 ttcgtttcgg gtgtttccag tcgatgccct caagaagcat ggataactgg gccggagtaa
     6361 ggtgcacctt gccgtcacgg gtgaccggcc agacgaagcg gccccgctcc aggcgtctgg
     6421 taaagaggca cagtccgtca ctgtcagccc acaacacttt tatctggtca ccccggcgtc
     6481 cgcggaagat gaacagatgc ccggagaacg ggtcatcctt caggacgttc tgaacttttg
     6541 aggccaggcc gttaaagcca tttcgcatat cggtgatacc tgcaaccagc cagatacgcg
     6601 aacctgcagg gaaagatatc atcagtggct gctccctttt atttcgcgga taagtatctg
     6661 taataacgcc ggcgtcagtt tacctttaag cctgagagtt ccggccggca gaaccagctc
     6721 acagcacaga ctgtcggacg gtgtatttat ctgctccggc gttaacgtca cgggaagcag
     6781 tgcaggcata ttttttccgg aaggcagcag gccacctttc cggtattgat ggcgccagtt
     6841 gaagagcagg ttatcgttga ttccgttttc acgggcgatc tgcgccacac aggctccg
//
LOCUS       DAJTRQ010000169         6783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000169 DAJTRQ010000000
VERSION     DAJTRQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            229..2661
                     /locus_tag="OO332_003753"
     CDS             229..2661
                     /locus_tag="OO332_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5944048.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2728..4188
                     /locus_tag="OO332_003754"
     CDS             2728..4188
                     /locus_tag="OO332_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5944049.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            4197..5858
                     /locus_tag="OO332_003755"
     CDS             4197..5858
                     /locus_tag="OO332_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5944050.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6079..6420
                     /gene="symE"
                     /locus_tag="OO332_003756"
     CDS             6079..6420
                     /gene="symE"
                     /locus_tag="OO332_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5944051.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6467..>6783)
                     /locus_tag="OO332_003757"
     CDS             complement(6467..>6783)
                     /locus_tag="OO332_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944052.1"
                     /translation="FDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
BASE COUNT         2004 a         1611 c         1547 g         1621 t
ORIGIN      
        1 aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgt ttcctgtaac
       61 ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa cagagatatc
      121 aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata gccaggctat
      181 tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat ggcggaactg
      241 aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc agccattctc
      301 aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg ggacggtaaa
      361 gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga catcgtgctg
      421 taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa acatgaaatt
      481 ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc ttttgttttt
      541 gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg tgaatgcctt
      601 gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca aaaattctgc
      661 ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta ttacgacgat
      721 ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac cattgaagcc
      781 gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg gaaaacctat
      841 accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg cattttgttc
      901 cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca gccatttggt
      961 acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat tcacctcgcg
     1021 ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca agtcgcacca
     1081 gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc cgaagacagc
     1141 gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt aaccgccacg
     1201 ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt ttacgtctac
     1261 tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt ccgtgttgat
     1321 attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt aaacggcgaa
     1381 gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt gatcgacgaa
     1441 cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa tccgatggat
     1501 aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg cgccctggtt
     1561 aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat caccggcgat
     1621 gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc gtacccggtt
     1681 atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg caaactggta
     1741 gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg tcgtggtaca
     1801 cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa aaaagccacc
     1861 gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga taccacacca
     1921 gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat cagcgaacat
     1981 gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca ggttaccgat
     2041 accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa gtttcacgtc
     2101 aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc cgacggtaaa
     2161 ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga atatgcctcg
     2221 ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct cattcacgag
     2281 ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa agatctcgac
     2341 ccgttcgaca ttctttgcca cgtagtgtat ggtcagccgc cgttaacccg caaagagcgc
     2401 gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc gcaagccgtg
     2461 ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga aagtattcag
     2521 gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat taaaaccgga
     2581 tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga aatctaccaa
     2641 ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat catgttgcag
     2701 aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa atcattacaa
     2761 gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg tcagctctcc
     2821 tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact ggagcaggat
     2881 aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc caacgcgcag
     2941 ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc ggcgttgaaa
     3001 aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca ggcgttcagc
     3061 gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa caagctgaac
     3121 gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta cgaacagatc
     3181 cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg cgccgtcact
     3241 cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga cccggcctgc
     3301 ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata cgtgaagagc
     3361 gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa acagcttccg
     3421 cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt acaaattcgt
     3481 cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga tgtcattgtt
     3541 accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt tccggcagag
     3601 atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt actggcgaaa
     3661 aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg tgttaaaacc
     3721 aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg tttaccgaat
     3781 ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac caaaggtcag
     3841 ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa aaactacagc
     3901 aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg gggtaacgag
     3961 gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag cattgatgac
     4021 gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga aaccgtcagc
     4081 catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat ccagacgctg
     4141 cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaagtaggt taactaatga
     4201 gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg caaacccgtt
     4261 ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag aaattgcgtg
     4321 agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg aatgatgaac
     4381 cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg aagcaaggca
     4441 aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg tttgaattgc
     4501 cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct gagtcatcta
     4561 gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc gagcaaagcc
     4621 aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta aataatccag
     4681 ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat tttgttgtcg
     4741 gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc tttttttggc
     4801 agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa gtattaaata
     4861 gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa aaagttagct
     4921 ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg gacgcacatc
     4981 agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc gcggaactgg
     5041 ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc gaagccagcg
     5101 tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt gtgccgcagg
     5161 atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa aaagctcaat
     5221 tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc gatgaggaaa
     5281 aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata gttaatatta
     5341 aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca cctgatatta
     5401 ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca ggagtcaaag
     5461 ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc ccgtcattat
     5521 ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat ccattagact
     5581 gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt cttatgcaag
     5641 tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt ttttacaata
     5701 ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag ataaattcta
     5761 ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa acccaactgc
     5821 atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct cacaaaacca
     5881 ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc acgctaattt
     5941 tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat gatatatcca
     6001 ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca caatcactat
     6061 ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt agaagcagaa
     6121 gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta cccggattac
     6181 agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg ttttgccacc
     6241 ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc ccaacccgcc
     6301 gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct gtcggcgcgt
     6361 aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa agtcgcctga
     6421 tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat accaacgcgc
     6481 tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga agttgtgcga
     6541 ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt gtcacatctt
     6601 tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac aatcgcagtc
     6661 ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca atatatagct
     6721 tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga agatgatcga
     6781 ata
//
LOCUS       DAJTRQ010000170         6778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000170 DAJTRQ010000000
VERSION     DAJTRQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..91)
                     /locus_tag="OO332_003758"
     CDS             complement(<1..91)
                     /locus_tag="OO332_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HCR5944053.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            147..299
                     /locus_tag="OO332_003759"
                     /pseudo
     CDS             147..299
                     /locus_tag="OO332_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1680..2627
                     /gene="icsP"
                     /locus_tag="OO332_003760"
     CDS             1680..2627
                     /gene="icsP"
                     /locus_tag="OO332_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5944054.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3189..3561)
                     /locus_tag="OO332_003761"
                     /pseudo
     CDS             complement(3189..3561)
                     /locus_tag="OO332_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3805..3924
                     /locus_tag="OO332_003762"
                     /pseudo
     CDS             3805..3924
                     /locus_tag="OO332_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4174..5040
                     /gene="ospB"
                     /locus_tag="OO332_003763"
     CDS             4174..5040
                     /gene="ospB"
                     /locus_tag="OO332_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5944055.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5369..6109
                     /gene="phoN2"
                     /locus_tag="OO332_003764"
     CDS             5369..6109
                     /gene="phoN2"
                     /locus_tag="OO332_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5944056.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6462..>6778)
                     /locus_tag="OO332_003765"
     CDS             complement(6462..>6778)
                     /locus_tag="OO332_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5944057.1"
                     /translation="KKADIALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         2131 a         1266 c         1315 g         2066 t
ORIGIN      
        1 caactctagg ataaatattt ctcactatat attcagtatt tgcagtattt attggaaata
       61 cgttcttaat attcttgatg ggactaatca ttttaatctc tatactcaaa tataaacatt
      121 accatgaaca atataattgt tcctaagtgg aatgtctcca cggagaacac tttatctatc
      181 gggaaatagt gcgggcaaca gtgtttaatt atatcgagtg cgattacaat cgctggcgac
      241 ggcaccgttg gtgtggcggc ctcagtccgg aacaatttga aaaccagaac ctcgcttagg
      301 cctgtgtcca tattacgtgg gtaggctcac agagcctcac ttacgactgc gtccatatta
      361 tgtgggtagg atcaataaag acgttttata tgtgtctgct tgcagccaga aaatttatac
      421 cctgcaggta tcataaacat gcttgataac ttaattgggg ctccctcttc cttttatggt
      481 gtaattgttt gattgaatac ttccggggat ttcagtatga aatgaagtat atttaatata
      541 ctttacaaaa agtttgtatt agtggaataa ggacaatagc ttatccaaac tgccaagcct
      601 ctttattata agtaagatct ggcactggaa tattctcaaa aggactaaca tcatcaccac
      661 gaccttcaac caaccatttt attgcattta aatcccagaa tagttcagta gtggtcaatt
      721 tgggaaacgc acaatattgt acggttacgt tatcgcaata gagtgaactc ccgccctata
      781 cggcgttttc aaggattagt ccttaatcgg acaaccacat taaagcataa taaataatta
      841 agctttaata atatactata gatacagttc tattataaaa aatactattt taggtgatgt
      901 attaaattag tagaatattt attcttttat aaaagcacca atgaattgtc caagtaaaca
      961 tgaggtaaaa tgtgtagagg ataactgagt ttggtcactt taactgtatt agtcgcagtc
     1021 tgactctcga ctttaaaagg atgggaatta cctgtactga tagtgagaaa gctttccatt
     1081 gaaataagtc aaataaaagt tgatggcttc agtgctaaag tgaggtgccc gctagccgta
     1141 ttcactcgaa tcggaattca atgtattgct tctgccattt ccataatatt tcacgggaaa
     1201 aacaaaatct gaaaaactat acttaagtgt tgtattaagt cagttgagat acaaatacat
     1261 atttatcagc acacgttacg tcaaatacag gaggttgtca tgttggtact gaaaggcacg
     1321 ttggctattg cagccgtgat gacaatttat tgtcttatcg ttgttcttat ggaacgcttt
     1381 tctgattgat ttcatattgg cgagagaaca agaaggtgag tagaattgtc gcggaatcct
     1441 gaaactatca gcctcaggta tgaacaccaa aaggtaggta gagaaatcta tggcccccgt
     1501 tactttattc gcacaatgag gtattttttt atttttacaa aagttcaata aatattataa
     1561 taaaagatat gttcttggta acataaacaa atatttaatt aaaataatgc ttggcattga
     1621 cgtttatctt ataaagtaag aagatcatgg acatttcaac caaaaaagta gagttctcga
     1681 tgaaatcaaa attccttgta cttgcacttt gtgtacctgc gatctttact acacatgcta
     1741 ccactaacta tccacttttc ataccggaca acatcagtac ggatattagt ctaggatctc
     1801 tgagtggcaa aacaaaagaa cgcgtttatc atcccaagga aggggggaga aaaattagtc
     1861 aactggactg gaaatacagt aatgcgacta ttgttagagg tggcatcgat tggaagctaa
     1921 ttccaaaagt gtctttcgga gtttccggtt ggactacttt aggtaaccag aaagcaagca
     1981 tggttgataa agactggaac aattccaata cccctcaggt atggaccgac cagagctggc
     2041 atcccaatac gcatctccgt gatgctaacg aattcgagct gaatcttaaa ggttggttat
     2101 taaataattt ggattatcga cttggactaa tagcaggtta ccaagagagt cgttacagtt
     2161 ttaatgcaat gggagggagt tatatttata gtgaaaacgg ggggaacagg aataaaaaag
     2221 gggcacatcc tagtggtgaa agaacaatag gttacaaaca gctcttcaaa ataccttata
     2281 ttggattaac tgctaattac cgccatgaga attttgagtt tggagcagaa ctgaaatata
     2341 gtggttgggt tctttcatct gatacagata aacactatca gactgagaca atttttaaag
     2401 atgaaataaa aaaccaaaat tactgctctg ttgctgcgaa tattggatac tatgtcaccc
     2461 ccagtgcaaa attttatata gaaggctcca gaaattacat ttctaataaa aaaggtgata
     2521 catctcttta tgagcaaagt accaatatat ctggcaccat taaaaatagt gcaagtattg
     2581 aatatattgg ttttctcact tccgcaggta taaagtatat tttttgaatt ggttcaccga
     2641 gttgattacg ttttcatggc taaaaattgc cgcccattgt gagtcatatt cgccctgact
     2701 ttccagaacc atacgaactg cccgttgacg gacttcgggg gaaaaacgtg tatttttagt
     2761 cattctgttt acctctttct cagggcgttt agtctccagg atttccgggg cggttcaaaa
     2821 aactcttcgt tgattactgt ggacttactg ttggcattac cgaccctgag accggagaaa
     2881 taagaaccgc ttagatcatc gtggctgttc tcggggtatc aagttacaca tgggcagagg
     2941 ctacccaagg tctactatta tcgcctgaaa gcgctgatgg aacagtgcta tcagggacat
     3001 gctgatgaaa atacctaaat aatcagccag cactctatct ttccaaatcc acagcatagc
     3061 aaagagagca aaagagcctg taaattcaga aattaaggaa acgcctcttg gtgcacataa
     3121 tctatcttat gttaaatgaa atctaatttc ttgattatat gctggatcgt tcgtgggtgg
     3181 ctttgcccct atatttccag acacctgtta tcattggccc gctgccgcag atattcccgt
     3241 ggcgagcgat aatccagcgc actatgcgga tgccattcgt tataatgctc gaatgcctct
     3301 gcaaggttct ttgctgccgt taacccgtca ggtttgggca tgatactgat gtagtcacac
     3361 tttattgttt tcacgaagct ctttgctatt ctgttactct ccggactccg caccgccgtg
     3421 ttcttcggtt caacaaagtc ccaacatccg ggtgaactgg cgtgtttcat tagcccggta
     3481 gcatgaacca ttatccgtca gtcactccac tggagacgac ggaagatcgt tgccgaagcg
     3541 gtgttccacc gctcccagca tgtcgccaat gggagagcat ctacggtctg attgaactgt
     3601 ttattcaaat tgtccaccgt ctgtcagtac gctctgaacg tcggctggtt aaaacactgc
     3661 tcgctgattt tcagagggtt cacggtaaaa cagcactttt attccggatt gcggaagcgg
     3721 ctctgaataa tccggatggg ctggttaaag aggttgtgta ccatctgcat attgttgaac
     3781 cagatcgcag cggtaagaga agttcatcat acttggcgca gttacgcgat gtttctgcta
     3841 gaggtgatgc cgtaaagaac ggcagaacat taccggaaca agatagcggt ctacctgcac
     3901 tggtatcaga cccggggctt ccccggatga tctccacggt actttaggta ctgtgtctat
     3961 attatgtagg taggatcaat taactttgta gttgaaattc tttcattgaa ataatgaata
     4021 aaaaagacaa accgcattaa acaatgccag aatataattg catttttgtt ccgttttttt
     4081 aaatcatagt atacaaagaa gcttgcatac tatatgaaaa tataacaccc tttactatga
     4141 tgtttataaa acaatatatg gagtcatgta ggtatgaatt tagatggtgt tagaccatac
     4201 tgtagaatag tcaataaaaa gaatgaaagc atatcagata ttgcatttgc acatataata
     4261 aaaagggtaa aaaattcatc atgtactcac ccaaaagcag cattggtttt tttaggagag
     4321 aaaggttttt gtgatagcaa tgatgttcta tctattatgg gacaacaaat accaagagta
     4381 tttaagaaca agatgttata tgattatgtt tttaaaaatg aaaaaagtaa aaatgatttt
     4441 ctaaaaatgg ctgaatcatg gctaccacag agtgaaccaa tagtaataaa taatgatgat
     4501 gacgcattga atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa aacagtaaac
     4561 gatactgatt ttaaagagta taataaggtt tatattcttg ggcacggtag tcctggttct
     4621 catcaattag gccttggttc ggaacttatt gatgtacaaa caatcatttc aagaatgaaa
     4681 gactgtggta ttctaaatgt gaaagatatc cgttttactt catgcggctc cgctgataaa
     4741 gtggctccta aaaattttaa caatgcccct gctgaaagtc tttcttgtat ccttaactct
     4801 ctgccttttt ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca ccttgaaaac
     4861 gatgagtcat tgagtgatgg tctaaaaata tccggctatc atggatatgg agttcactat
     4921 ggtcaagagc tttttcccta ctcacattat cgttcaactt caattcctgc tgatccggag
     4981 catacagtaa aaagaagctc tcagaaaaag acttttatta ttaataaaga actggattag
     5041 tataaaattt ttaacctata gttaattgtt tgcatttaag atgaagttat tttatcttta
     5101 gtagaaaagt gcttccctat gagggaaaat cacaaatcat cataatacag agacaaaacg
     5161 atacgaacaa atagataaaa aacatcattc ttttaccaca ttatttttcg tgaatatgaa
     5221 aaataatgtt attactttaa tataagacta ttttttgttt ttccatcact ctgttcaaat
     5281 ttttccgcat gacttgtgtt ttttgtaaca cagctcgttt tttacagctg accaaaatca
     5341 tcaattaatt atgctaagga aataaattat gaaaaccaaa aactttcttc ttttttgtat
     5401 tgctacaaat atgattttta tcccctcagc aaatgctctg aaggcagaag gttttctcac
     5461 tcaacaaact tcaccagaca gtttgtcaat acttccgccg cctccggcag aggattcagt
     5521 agtatttcag gctgacaaag ctcattatga attcggccgc tcgctccggg atgctaatcg
     5581 tgtacgtctc gctagcgaag atgcatacta cgagaatttt ggtcttgcat tttcagatgc
     5641 ttatggcatg gatatttcaa gggaaaatac cccaatctta tatcagttgt taacacaagt
     5701 actacaggat agccatgatt acgccgtgcg taacgccaaa gaatattata aaagagttcg
     5761 tccattcgtt atttataaag acgcaacctg tacacctgat aaagatgaga aaatggctat
     5821 cactggctct tatccctctg gtcatgcatc ctttggttgg gcagtagcac tgatacttgc
     5881 ggagattaat cctcaacgta aagcggaaat acttcgacgt ggatatgagt ttggagaaag
     5941 tcgggtcatc tgcggtgcgc attggcaaag cgatgtagag gctgggcgtt taatgggagc
     6001 atcggttgtt gcagtacttc ataatacacc tgaatttacc aaaagcctta gcgaagccaa
     6061 aaaagagttt gaagaattaa atactcctac caatgaactg accccataaa gctggacagc
     6121 ctgtatcagg ctatggaggg cccatagaca aatctaccct atatgagcat aggaggagtc
     6181 tatgggcaca ccacgtttta ctcctgaatt taagggatta ctggaaaggc tgggacatat
     6241 ccctccggca gaagcagaaa aagcttatta tgctgccatc ggaaacgatg atctggcaac
     6301 ctgagttcac agataaaaca ttctctagga aactcggggc ggttccgttc accacatgca
     6361 atgtggtgtt gcaggggaac ggtctgcacc atcccctata tcgatcaata taacaaaaat
     6421 gacaacttca gattcagggc acaacctaaa tatattttag gtcacctctc aaatcgtttg
     6481 cctgataccg ctccattttt taataaaaaa tcaatcattt ttgaatcttt gcttttcatt
     6541 gcattgtcaa gcatagtgtc cccactattt gctttttgaa attttttgtt tacatcaacc
     6601 aatccattat tgataaaata ttctagcact ttatagttgg caccatcttt actaagtaag
     6661 tattcaacat catacagatt acaatatata ttgttgttca ttttctccat ctctgcgata
     6721 tcttgttttg taaaattaaa tttagatagt aaataaagtg ctatatcagc ttttttat
//
LOCUS       DAJTRQ010000171         6770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000171 DAJTRQ010000000
VERSION     DAJTRQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..3513
                     /locus_tag="OO332_003766"
                     /pseudo
     CDS             1..3513
                     /locus_tag="OO332_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3523..3766
                     /locus_tag="OO332_003767"
                     /pseudo
     CDS             3523..3766
                     /locus_tag="OO332_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            5305..6770
                     /locus_tag="OO332_003768"
                     /pseudo
     CDS             5305..6770
                     /locus_tag="OO332_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1794 a         1779 c         1629 g         1568 t
ORIGIN      
        1 cgaaagataa ttctttgcaa ccttcccaaa cggtaacgga ctctctcaag gtagcggact
       61 tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg gctgacaaaa
      121 atacagatgc tgttttacct tggctggcga aagatattgc tgtcgttttt ccgcccgaag
      181 ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag caagcagaca
      241 aattgaccca attacgtcgc ttagcctgta gcgtgtcgca acgggataat aaaacagcca
      301 catttgactt cagcacctgt tctttagaat ggcaaaacac cgtcgcccaa gcgatcagtc
      361 agatcgacgg cctgaaaaca acacagctac catcaccagt aatggctgta ctcacggcac
      421 ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag atcgtccagg
      481 aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt gatattaaat
      541 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
      601 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      661 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      721 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      781 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      841 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      901 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      961 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
     1021 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
     1081 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agaacaggtc attccctggc
     1141 tttcgacacc cgcagttgca gtgctgaaat catgccagca ccaactgaca cagccctcaa
     1201 accatgccag cgccgatcta ctgccagcca tagtagtctc cccaccatgg atttcgaaaa
     1261 agaaaaaatc gccgattccg gtgctggatt tagcgccatt aggcatcgag ccaatctgtt
     1321 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
     1381 tcacggttag ccatgaagaa agtactacca acctgttggc aaggatgggt tttcaacgac
     1441 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
     1501 attattcaac cttactaagt gaatttaagg tgtttcattc acctaccggg cattattggc
     1561 agttggggat tttgacaaca ttgccgctgg aggctggaga aagcagtaaa agcatggaat
     1621 gcccttactc tatctccaca taccgatacc gaatacgcca tgttacattt tggactcaaa
     1681 ggtttacctg gtttagttaa ctcaattaca cgctatccgc aggaagcctt gcccatcacg
     1741 aattacttcg cagcgagtga gctggctcct gccgtcgccc gtgccttcaa caaactcaaa
     1801 acgctacgcg aaaacgcccg cacctggctg ttgaaatacc cggaacatgc ccttaccggc
     1861 ctgctgcctg cggcgctcgg caaagccggt gaagcacaag ataacgcccg cgctgccttg
     1921 cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag aaatcgcccg gcgttataac
     1981 cagtcggaag taacagacgc ggtgaacgct ctgcttgcgc tcgatccctt agataatcac
     2041 ccgacaaaaa tccccactct tccgaccttt tatcagccat cgatctggac gcgcccggta
     2101 ttaaaagcaa acgcccaatc actgccagat aacgccctcc tccacctcgg cgaaatgctc
     2161 cgcttccctc aggaagaggc tctgtatccg ggattattgc aggtgaaaga cgcctgtacc
     2221 gctgactctc tggctgaatt tgcctgggat ctgtttaccg cctggcagac cgctggcgcg
     2281 ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc
     2341 cgcaaactga cgccattaat acgcgcctgg cctggtgaat cccagcataa acgcgccacc
     2401 gttgggttgg atattctcgc cgctatcggt agtgatatcg ccctgatgca gcttaacggc
     2461 attgctcaga aactgaaatt caaagcatta caggagcggg caaaagaaaa gattgccgac
     2521 attgccgaga gccgcgaact cacggtagcg gagcttgaag atcggttagc accggatctc
     2581 ggtctggatg ataacggttc gttgctgctg gattttggcc cacgccagtt caccgtcagc
     2641 tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg
     2701 ccaaaaccaa acaaaagcga tgatgagtca caggcgaacg atgctgttaa ccgctacaaa
     2761 ttgctgaaaa aagatgcgcg taccgtcgcc gcccagcagg tagcaaggct tgaatccgcc
     2821 atgtgcctgc gtcgccgctg gtcgccggaa aacttccaac tcttcctggt tgtgcatccg
     2881 ctggttcgcc acttaacccg ccgtctgatt tggggcgttt atagcgccga caaccagcta
     2941 ctggcctgtt tccgggtagc ggaagataac agctacagca ccgctgacga tgatcttttc
     3001 accctgccgg aaggcgatat ctctatcggc attcctcacg ttctggaaat atcaccgacg
     3061 gatgctgtcg cctttggtca gctttttgcc gactacgaac tgctaccacc gttccgtcag
     3121 cttgaccgta acagttacgc cctgacagaa gccgagcgca acgccagcga actgacccgc
     3181 tgggcaggca gaaaatgccc gagcggccgg gtcatggggc tggcaaataa aggctggata
     3241 aagggcgaac cacaggatgg aggctggatc ggctggatga tcaaacctct cggtcgctgg
     3301 tcgttaatca tggaaatcga cgaaggtttt gcggtgggta tgtcaccagc cgaacttagc
     3361 gctgagcagc tcttaagcaa gctgtggcta tgggaaggca aagcggaaag ctatggctgg
     3421 gggagtaatt caacacagga agcgcagttc tccgtactcg atgccatcac cgccagcgag
     3481 ctaattaacg atattgaagc cctgtttgaa taaggaaaga gcatggacaa ggaattaccg
     3541 tggctggcgg ataacgccca actggaactg aaatataaaa aaggcaaaac accactcagt
     3601 catcgcaatt ggccgggcga accagtgcct gttatcactg aaagcatcat tcagacactg
     3661 ggtgatgaat tgctacaaaa agctgagaag aaaaaaaaca ttgtctggcg ttatgaaaat
     3721 ttttcaccgg agtggcagtc cgccattacg caggccatca acttgatcgg cgaacacaaa
     3781 ccctcagtcc cggcccgcac aatggcggca ctagccttta tcgcgcaaaa tgacaaccaa
     3841 tagttgctcg acgaaatcgt ccaacaagag gggctggaat atgcgactga ggtggtgatt
     3901 gcacgccagt ttattgcacg gtgttatgag agtgatcctc tggtagtcac attgcagtat
     3961 cagaacgagg actatggcta tggttatcgc tcagaaacct ataacgaatt cgatctccga
     4021 ctgcgtaaac acctctctct ggcagaggaa agctgctggc agcgttgcgc cgacaaactc
     4081 attgccgcac tgccaggaat aaccaaagtt cgccgccctt ttattgcgct gatcctcccc
     4141 gaaaaaccag aaatcgccaa tgagttggta agccttgaat gcccgcggac tcattttcat
     4201 tctaaggagt ggttaaaagt tgttgctact gaccccaaag cggtgagaaa actcgaacgc
     4261 tactggagcc aggatatatt tagcgatcga gaagccagct acatgtcgca tgaaaaccac
     4321 ttcggctacg cggcctgcgc tgcccttttg cgcgaacaag gactggcagt cgttccgcgc
     4381 ctcgcgatat atgcccataa agaagattgc ggcagtctgc tggtacaaat taaccatccg
     4441 caagtcatcc gcaccttgct gttagttgct gataaaaaca aacccagcct gcaacgtgtg
     4501 gctaaataca gtaaaaactt cccccatgcg acactcgccg cactggcaga actgctggcg
     4561 ttaaaagaac caccagcccg cccaagttat ccaatcatcg aagacaaaaa gctgcctgca
     4621 cagcaaaaag ctcgagatga atactggcgt acgctgttac aaacgctgat ggcatcgcag
     4681 ccacaactgg cagcagaagt gatgccgtgg ttaagtactc aagcccgggc agtgctgaat
     4741 agttatttat cggcaccgcc caaaccagtt attgatagca ccgataacag ccaaatgcct
     4801 gaaatcctcg tttcaccacc atggcgtggg agaaagaaaa agacttccat acgtcacttt
     4861 tcgctgcctg atcttacgct tgcaccgcaa acgtacaggt cgccgaataa acactggacg
     4921 ccggaacagc aaactgcaat tgaccatttt agcgccatgt cttttgacga acgtcttgct
     4981 acgagaggag cggatatctt cttgcgggaa ttaggttttg acgttcatag cctggaattt
     5041 aataagcgtc ttcttaatgg acagttggat gcctttgatg ctcagcaact gcaagaatac
     5101 acatcaatag gactattgcg tacttatcac gcgcatcagg ccaaatctaa acagtaccgg
     5161 gaagacgcca ccaatgcatt actggcacaa gatagcattg cgttgatggg agggtggcga
     5221 acttttcagc acggatttta ctatggtaat gaacagcgtt gggggatttg gaatctgtac
     5281 cttatcgcgc agatgccgcg cgaaatggcg gtatcgtgct ggcaacaaat tgtcgaggct
     5341 gattttcatt acaccggggt ggaatatttg ttaagtgtgc tgggtaccga tgctttgcct
     5401 ggattaaaca ccgcctttgc ccgacatcca aaagagatat ttccgctgct gattcatttc
     5461 ggcgcaacag aactggccct gcccattgcc cgcgtctggc acctttttgc gggccagcgt
     5521 aatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc acttatccct
     5581 cttgtcttca ccaaatctag cgataaaagc gaagctgcat tacttgccct gcgtttactt
     5641 tacgaacatg ggcatggcga attgctacaa actgtagcca atcgttggca gcgtacagat
     5701 ctatggcctg ccctggaaca attacttaaa cagagtccaa tagagattta cccggcacgc
     5761 attccaaaag cccctgattt ctggcatccg caaatgtggt ccaggccgcg ccttatcact
     5821 aataatcaac ctgttaccga tgacgctctg gaaattatcg gcgaaatgct gcgctttacc
     5881 caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca gccacaaacg
     5941 ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc ccccgcaaaa
     6001 gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc acgggatcta
     6061 acgacacata tcctcgcctg gccacaagaa ggcaaatctg cccgtgcggt cagtggcctg
     6121 aacatcctta ccctgatgaa taatgatatg gcgctgatgc agctgcatca tatatcgcaa
     6181 cgggcgaaat cccgcccctt acgtgataac gcggcggaat ttcttcaggt agtcgcagaa
     6241 aatcgcgggc taagccagga agagttagcg gacagattag tcccaacact gggccttaat
     6301 gatccgcagg cgttgagttt tgattttggt ccccggcagt ttaccgttcg cttcgatgaa
     6361 aacctcaacc cggttatctt tgatcagcaa aacgttcgcc agaaaagtgt tccccggttg
     6421 cgcgccgatg acgatcaact gaaagcgccc gaggcactgg cccgactaaa agggttaaaa
     6481 aaagatgcta ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc cctacgtacc
     6541 acccgacgct ggtcgctggc agattttcat tctctgtttg ttaatcatcc ctttacccgt
     6601 ctggttaccc agcgattaat atggggggtt atccggcaaa tgaaccgcgt cgtttactca
     6661 acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
     6721 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagca
//
LOCUS       DAJTRQ010000172         6748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000172 DAJTRQ010000000
VERSION     DAJTRQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            526..1704
                     /gene="ltrA"
                     /locus_tag="OO332_003769"
     CDS             526..1704
                     /gene="ltrA"
                     /locus_tag="OO332_003769"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5944058.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1787..1966
                     /locus_tag="OO332_003770"
                     /pseudo
     CDS             1787..1966
                     /locus_tag="OO332_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1996..2478)
                     /locus_tag="OO332_003771"
                     /pseudo
     CDS             complement(1996..2478)
                     /locus_tag="OO332_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2734..3714)
                     /locus_tag="OO332_003772"
     CDS             complement(2734..3714)
                     /locus_tag="OO332_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5944059.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3714..4913)
                     /locus_tag="OO332_003773"
     CDS             complement(3714..4913)
                     /locus_tag="OO332_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5944060.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5235..5573)
                     /locus_tag="OO332_003774"
                     /pseudo
     CDS             complement(5235..5573)
                     /locus_tag="OO332_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5640..6421)
                     /gene="istB"
                     /locus_tag="OO332_003775"
                     /pseudo
     CDS             complement(5640..6421)
                     /gene="istB"
                     /locus_tag="OO332_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            complement(6418..6748)
                     /locus_tag="OO332_003776"
                     /pseudo
     CDS             complement(6418..6748)
                     /locus_tag="OO332_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1783 a         1570 c         1623 g         1772 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtggaggtgg gccctccggt cagggtttac
       61 aggagcactg accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt ggggttcggg aagctgaacg aaagtaaact
      181 gtccgatgaa gcatcgaaat cttagatgat gccaaaatgg ggccgtacag ccgtcccctg
      241 ataagcgcag aagacacctg cttactgact gcgcggcatc cggtgtgaag gcagcaggat
      301 cccgggccag accatctcag ggaacgtgag aacctgtttc cggcacgtat cgggaaacag
      361 gggcggaccc attcgtagta gtgatgaaag atctgtaatg ggtctggagc gaagggatgg
      421 ggatcacatt cattgcagaa gacacaaccc aaaaggggat gacgtaactg atgcaacgaa
      481 aatcatttga aataccaaaa gcactggtct gggcatcata tctggatgtg cgtcgcaaca
      541 agggagcccc ccggatgcga cgggcagact ttgaaaatgt tcgaccagca acgtgatggc
      601 aatctataca agatctggaa ccggctatgt tcgggaacat ggtttccgcc accagtgctg
      661 gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc tggggatccc aacagtatcc
      721 gatcgaattg ctcagggagc gataaaactt ttcatggaag aaaagcttga cccgattttc
      781 catgctgatt catatggtta ccgaccaggc aaatcagctc atgacgctct gaaacaatgt
      841 gccatccggt gctggcgtta tagctggata ctggaagtgg acatcagtgc gtttttcgac
      901 catgtgagac atgatctggt gctcaaagct ctggaacacc acgggatgcc taaatgggtc
      961 atcctgtact gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa tggagaatta
     1021 ataacccgaa cacgaggcac tccgcagggc ggggtcatta gtccgctact tgctaacttg
     1081 ttccttcact atgcatttga tttgtggatg gaaagagaat atcggggggt accgtttgag
     1141 aggtacgctg acgatattgt agtacattgt tcacgaatga gtgatgtaac aaggctaaag
     1201 aacaggttat cggagcgctt ctcggaagtc gggctggtcc tgaatgcagg gaaaacgaat
     1261 atcgcctaca ttgacacgtt taaaaggaga aacgtcgcaa cgagtttcac ctttctcgga
     1321 tatgacttca aagtgcgtac gctgaagaat ttcaaaggcg aacggtaccg aaaatgcatg
     1381 ccgggtgcgt caaatgcagc aatgcgcaaa ataacagaaa caatcaagaa gtggcgtata
     1441 catcgctcaa cagctgagag tttgctggat tttgcgaggc gctacaatgc gatagtgaga
     1501 ggctggatcg agtactacgg aaagttctgg tccagaaatt tcaactatcg actgtggagt
     1561 gcaatgcagt cacgtctgct caagtggatg cagtctaaat acagactttc gaaccggaag
     1621 gctcagcgaa agctgacgct ggtaaggaag gagtatccga agctatttgt tcactggtat
     1681 ttactacgtg catcaaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1741 tccgtgagca gccggggagt gaaattctct ccggctgact cgactcgcgg gttccgacag
     1801 cgatgacgaa catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt
     1861 ggaaccagaa aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg
     1921 ggtacgtgat ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt
     1981 ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct ttactgaaca caatgccgta
     2041 ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc ctttccattg aaaccaaccg
     2101 acgcatattc aaaaaagtct ggacgtcctg ttgctccaga gtttttattg gtacgggtcg
     2161 tatagttcca tgttggcagg cctcagctat tgcctgtgcg tcgttataat catttttctg
     2221 gcaccgctga taagctttaa catgttgtgc cggaagcaat attacatcat ggtcgagttt
     2281 cctgatttcc cgggcccagt aatgagatgc cccgccagcc tccatcgcaa cagtcatttt
     2341 aggctgttgc acctgccagt taagcagttg attgcgagaa agtttggtct ttcctgcggg
     2401 tttgccatga gcattgatac tgaaaagata aaagttcgtt ttagccagat caatgccggt
     2461 gattttctgt tcagacatgg tatcccgctt acttttgtgg ttaaagttta tccatcatgg
     2521 cgcaattgaa ggctgccgtg aaagaggggg aagactatct catcggttac tgttaaacgg
     2581 ctaagagaaa ctatcttttt caagcaaaga agatacggtg aaacaggcga tttcaccgtg
     2641 aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc ttttaaaaca gccaattaaa
     2701 aactggtgag ttgctggcga aaaacttgaa ttttcacagc gaattcagat tatcttttag
     2761 tacaagagca atcatacgat ctaacttatc ctgaacttcc aacggtaatc tgccgaattc
     2821 atatgagaac gtacgtccct ttactcgttt tcgagcaaat ttatctttac tatcaaattt
     2881 ccataacaaa gttacaaccg ctttatcctt tactcctgaa tcaagcaatg aagacatttc
     2941 cttggttata agccgcataa tactgttttt aacctcgtct tcagaaagat tgtcatcagc
     3001 agtaataagc tcaatactcg gcgagatatt cttcacaact tcatcaatag atatatcacc
     3061 cagcctcagt cgttcactta attcagccag ttgcctgtaa tcggcgtatg tcagctcaga
     3121 ctgaacagga aaaagagaca caaaatcttt aggtacggac gctgcttgga gggcacgagt
     3181 caccttagct gccgacaatc cctctctttc cgcaatttgc ttctggttca taccggcctc
     3241 tttgagacgt actaaacgca acccaatttc acgaatatta tgttcaagtg acgtctgcaa
     3301 atctttcgcc agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg
     3361 taatccaacc ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat
     3421 aagatcgcca gtccgaatcc caatacatgg atagaattgt tgtaaacgta tagttctggt
     3481 gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac
     3541 cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt
     3601 tcggccagta ttgagggtaa atacatgaac tggagccgat atttcttcag tattattcag
     3661 gatatttgta ttcagcgtac ggccaatagt cggtcggtgc tttctgtttt ccatcagtag
     3721 ttggccctta taaattcaat acgatcaaaa actgctttag cgaaatcttc tgctgccata
     3781 cgtgcatttt tcaaagcttc accactgcca acataagtaa caggattcgc agatatcacg
     3841 gtatcaaacg actcgccgct tctctcaaaa ccatcaagac gtggcattga aacatcaagc
     3901 atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt atttatgatc tgacttattc
     3961 gccagtttcg acatgaagcc aatatttgcc tgcaaacggc atgaacatcc atcctgttca
     4021 ataatctgta caagttcagg taaacgagcc aaatatttaa gcgtggaatg gaaatcaact
     4081 tgtgctggcg gcaccggtgt aaacatgata tctgcggcag caatggcatt tttcaaaaat
     4141 gcgtccagat gcgggcctgt atcaataagg atgaagtcga aatcatgctt cagcttatca
     4201 ataatattct cacgcaatat cgcgtgtttg ttttgtccta atagatgctc ctcgcacaga
     4261 gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag ccggcataac ataaacgcca
     4321 gggataactg acggaacgat aaaatcttcc agtaattcct cacgactcac gttctgcaac
     4381 atagcctgcg gtgcggttgt atcaaccagc cccaccgcat gcaagtagtt cagaaacata
     4441 gtggctgatg actggggatc taaatcaagg agcagaatac gtaaatcttc tgacagcagg
     4501 tgaggatgag cacgcagggc gtgagctacg gaaacactac tcacggtttt agacacaccg
     4561 ccttttagac taccgatgaa tatggaatat gcctcactat aacgatcgcg atatttagga
     4621 atgccacgat gtgcatagat atcgataatg ttctggatgg ttaatgcata ttttgtcgcg
     4681 gttcctgctg ggcgtttttc aaactgatag ccctgagcct ccatctcacc gactgcacca
     4741 tccacgcttg cgcgcgttaa ttttggaagc ttagctacag cagactttga atagacttga
     4801 taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa tctgttttgt tagggcgagc
     4861 aacattttat ctgcacgctg cgcaacctta cttaactgct caaaactagt cattccccct
     4921 ccgtgctatg taggatttta acttattaca aaaaaaactg cattagcagg gtaatgcatg
     4981 caagtacata ttgtacatgc ttatattaaa aatgcgcgca ttgctgattt attaatcatc
     5041 ggaatgaata ttactgtaag atagtatgcc tttccgggag aaagacgctg atttcccccg
     5101 aataatccag ttcaccacca cactccggac aacagctttc ttcaggctcc agatggtgta
     5161 tttcgcggga aacatgttcc ggtaacggac ggcgatgacg tgactgtcgc aacaggcggg
     5221 ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga aggagccgat gactttttcg
     5281 tggatttcca cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcgggtg
     5341 cagagggtcc ggttgttacg ttcaatacgt tgcgtgaaga ttttgccggt caggtgtttc
     5401 tccttcggca cttctctggc gtagctgccc cagtcatcgc tggtcaccat gccgatacag
     5461 aacggagtca acagggctaa cagttcccga caggtctcat cattccgtgg cccgaaagtg
     5521 taagccaaca caccgccagt tttagtgttg tacgcatacc agagccagtg ttgtgtcaac
     5581 gacggatgaa aagtgctcca cttatatctc caccaacggc ccaatattga tccaccgttt
     5641 tactcaggat tagcttctgc tataaccccg gcctttcgtt tctgtctgag tcgatagctt
     5701 tctcctttga tttgaacgac atgtgagtgg tgtaagatac ggcccagcat cgctgaggtc
     5761 agtgctgcat caccggcgaa cgtttgatcc cactgcccga acggtagatt ggatgtcagg
     5821 atcattgcgc tcttttcgta acgtttagcg atgacctgga agaacagttt tgcttcttcc
     5881 tgactgaacg gcagatagcc tatttcatca atgatgagca ggcgggggcc attactccac
     5941 gctgaagcgt cgttttataa cggccctgac gttgtgccgt agataactga agtaacagat
     6001 ctgctgctgt tgtgaagcga actttgatac ctgcacggac tgcttcatag cccatcgcta
     6061 ttgccagatg ggttttcccc acacctgatg gccccagtaa tacgatattt tcattacgtt
     6121 ctatgacgct gagtgagcgt aacgactgga gttgcttctg cggtgctccg gtggcgaatg
     6181 tgaagtcata ctcttcgaac gttttcaccg ccgggaaggc tgccattcgg gtatacatcg
     6241 cctgtttacg ttgatgacgt gccagttttt cttcatgaag cagatgctcc aggaagtcca
     6301 tataactcca ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg cttataaggc
     6361 tttccagttg caactgcccg gcgagcgcca tcagtcgttg atgttgcagt tccatcatca
     6421 cgccactcct ctgcagaatg agtcgtagat ggagagtgga tgatgctggg ggggttgtcg
     6481 aagctcacca gattttcacc aggatgcacg tcatactctt ttttctccgg aggcagtgcc
     6541 agcatggact gctgctcttc gagccagcga tcacagggac gggcctggat tgtttcatgc
     6601 tttcgttggt tagcgacatc gtgcagccag cgcagaccgt ggcggctggc tgtttcaaca
     6661 tcgacagtgc tccccatcgg gcgcaggcga gtcattagtg ggatgtaaaa actgttacgg
     6721 gtgtactgca ccatccgttc caccttac
//
LOCUS       DAJTRQ010000173         6709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000173 DAJTRQ010000000
VERSION     DAJTRQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            289..438
                     /locus_tag="OO332_003777"
     CDS             289..438
                     /locus_tag="OO332_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944061.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            639..2222
                     /locus_tag="OO332_003778"
     CDS             639..2222
                     /locus_tag="OO332_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5944062.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2674..4527)
                     /gene="asmA"
                     /locus_tag="OO332_003779"
     CDS             complement(2674..4527)
                     /gene="asmA"
                     /locus_tag="OO332_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5944063.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4549..5130)
                     /gene="dcd"
                     /locus_tag="OO332_003780"
     CDS             complement(4549..5130)
                     /gene="dcd"
                     /locus_tag="OO332_003780"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5944064.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5222..5863)
                     /gene="udk"
                     /locus_tag="OO332_003781"
     CDS             complement(5222..5863)
                     /gene="udk"
                     /locus_tag="OO332_003781"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5944065.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6181..6603
                     /locus_tag="OO332_003782"
                     /pseudo
     CDS             6181..6603
                     /locus_tag="OO332_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
BASE COUNT         1574 a         1746 c         1689 g         1700 t
ORIGIN      
        1 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
       61 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
      121 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
      181 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
      241 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
      301 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
      361 acaattcggc aattggctat ttttaagaat tatattaagt gtcattcaat atggttttta
      421 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
      481 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
      541 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
      601 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
      661 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
      721 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
      781 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
      841 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
      901 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
      961 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     1021 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     1081 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     1141 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     1201 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     1261 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     1321 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     1381 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     1441 ccgaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     1501 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
     1561 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     1621 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     1681 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     1741 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     1801 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     1861 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     1921 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     1981 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     2041 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     2101 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     2161 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     2221 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     2281 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     2341 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     2401 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     2461 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     2521 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     2581 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     2641 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     2701 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     2761 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
     2821 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
     2881 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
     2941 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
     3001 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
     3061 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
     3121 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgccc
     3181 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
     3241 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
     3301 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
     3361 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
     3421 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
     3481 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
     3541 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
     3601 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
     3661 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgccc
     3721 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
     3781 ctcctgccac tgggactgga aactcccctg tccctgaata ccttgttttg gtaaatcggc
     3841 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
     3901 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
     3961 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
     4021 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
     4081 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
     4141 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
     4201 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
     4261 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
     4321 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
     4381 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
     4441 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat
     4501 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
     4561 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
     4621 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
     4681 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
     4741 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
     4801 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc
     4861 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
     4921 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
     4981 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
     5041 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
     5101 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct
     5161 taacgccaat tacgtcaagg gcataccagc ttatcactga acggtacaca atttatcaag
     5221 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
     5281 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
     5341 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
     5401 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
     5461 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
     5521 tgaccttctt cggctcaacc ttcaccgttt ctttcatacg cgtatgttca acatagctgt
     5581 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
     5641 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
     5701 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
     5761 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
     5821 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
     5881 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
     5941 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
     6001 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
     6061 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
     6121 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
     6181 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
     6241 agtttaggtt tagtctcgtt tatctttact cttgtctcgc ttgagctttc gcagtttggc
     6301 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
     6361 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
     6421 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
     6481 gccgtggtcg gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat
     6541 ctggctgact ggctgcgtct ggcattcggc agcgccattg ttccgcctct gttggggggt
     6601 gcccctatta agtagtcaag catgttatga cccctgcggg gtataaaaag tcccgtcgcg
     6661 catcatggcg aacagaacgt cgcagcgtcg tctcgccagg gcgataagc
//
LOCUS       DAJTRQ010000174         6670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000174 DAJTRQ010000000
VERSION     DAJTRQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1396
                     /gene="sbcB"
                     /locus_tag="OO332_003783"
     CDS             <1..1396
                     /gene="sbcB"
                     /locus_tag="OO332_003783"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5944066.1"
                     /translation="LFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPAD
                     DYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTR
                     NVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTK
                     ANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPL
                     AHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDIDTLRERLYTAK
                     ADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVRE
                     KVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRI
                     EKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLVQQYADDKEKV
                     ALLKALWQYAEEIV"
     gene            complement(1439..1666)
                     /locus_tag="OO332_003784"
     CDS             complement(1439..1666)
                     /locus_tag="OO332_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5944067.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1680..2738)
                     /gene="yeeE"
                     /locus_tag="OO332_003785"
     CDS             complement(1680..2738)
                     /gene="yeeE"
                     /locus_tag="OO332_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5944068.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2917..4275)
                     /gene="plaP"
                     /locus_tag="OO332_003786"
     CDS             complement(2917..4275)
                     /gene="plaP"
                     /locus_tag="OO332_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5944069.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4265..4345)
                     /gene="yoeI"
                     /locus_tag="OO332_003787"
     CDS             complement(4265..4345)
                     /gene="yoeI"
                     /locus_tag="OO332_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5944070.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4543..5472)
                     /locus_tag="OO332_003788"
     CDS             complement(4543..5472)
                     /locus_tag="OO332_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5944071.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5518..6342)
                     /locus_tag="OO332_003789"
     CDS             complement(5518..6342)
                     /locus_tag="OO332_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5944072.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6425..6670)
                     /gene="yoeB"
                     /locus_tag="OO332_003790"
     CDS             complement(6425..6670)
                     /gene="yoeB"
                     /locus_tag="OO332_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5944073.1"
                     /translation="MWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEP
                     LKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1792 a         1758 c         1571 g         1549 t
ORIGIN      
        1 cctctttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt
       61 cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg
      121 caagcccgcg gatgactatt taccccggcc tggagcagta ttaattaccg gtattacccc
      181 gcaggaggca cgggcgaaag gagaaaacga agccgcgttt gccgcccgta ttcactcgct
      241 ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt
      301 cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga
      361 taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc gcccggaagg
      421 aataaactgg cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa
      481 agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat
      541 tgcgatggcg aaactggtaa aaacgcgtca gccacgcctg tttgattatc tctttaccca
      601 tcgtaataaa cacaaactga tggcgttgat tgatgttccg cagatgaaac ccctggctca
      661 cgtttccgga atgtttgggg catggcgtgg caataccagc tgggtggcac cgctggcgtg
      721 gcatcctgaa aatcgcaatg ccgtaattat ggcggatttg gcaggagaca tttcgccatt
      781 actggagctg gatatcgaca cattgcgcga gcgtttatat accgcaaaag ccgatcttgg
      841 cgataacgcc gccgttccgg ttaagctggt gcatatcaat aaatgtccgg tactggccca
      901 ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct
      961 cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tagcgatatt
     1021 cgcggaagcc gaaccgttta caccttcaga taatgtggat gcacagcttt ataatggctt
     1081 tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt
     1141 accggcactg gatatcactt ttgttgataa acgaattgaa aagttgttgt tcaattatcg
     1201 ggcacgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc aggagcaccg
     1261 ccaccaggtc ttcacgccag agtttttgca gggttatgct gatgaattgc agatgctggt
     1321 acaacaatat gccgatgaca aagagaaagt ggcgctgtta aaagcacttt ggcagtacgc
     1381 ggaagagatc gtctaatttg aagccgccgc cgctggtacg gcggaggaca atgttgagtc
     1441 aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg
     1501 catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggta caatcaaact
     1561 caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg
     1621 gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact
     1681 taatttgccg cagcagttgc cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag
     1741 gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg ccaggtcatc
     1801 attgcagtca taaccagtcc attaccgata gagcaaccac ctgcaatact ggcaccgaag
     1861 cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc tgcgcgaacg
     1921 cggaactcac ggctcgcttt ggctgcaata aatgccccca cgaagatccc taacaccagg
     1981 aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc
     2041 gttggggaag tgactcctaa cccaaacatg cgtccggttg cttcactcag gggccaggct
     2101 aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg tttttcaaac
     2161 agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc
     2221 ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggtaac caacggccac
     2281 acagacaaat taaaagtctc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt
     2341 tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa
     2401 agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat
     2461 ccacccgcca gaacaattcc cagcccgaaa agatacccac ctataacagt accaagccac
     2521 gggaacgccc cggcttcgta agtcagtagg cccgccagaa ttaacgcaaa aacccccacg
     2581 ctttgtgccg aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac
     2641 atatcgcgaa aaccaccagt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga
     2701 gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg
     2761 ttaatgggga aatttttcat gactggtgga atgcagacca ataaccaaat tctctaaatt
     2821 agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc ggagccaaat
     2881 gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt cgtactgcgg
     2941 aaccggatta cggaagctct tggtgacgca agccaggtaa atcagaccga tagcagccca
     3001 gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag
     3061 cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct tgttacgctt
     3121 ctcacggatc cagaactgcg agatgaccga caggttaaca aaggtgaacg ccaccagcgc
     3181 accaaagtta atcagcgccg ttgccattac caggtcgaag ttgattgcca gcagggcaat
     3241 cgcgccaacc aggatgatgt tcatcgccgg agtacgccat ttcgggtgta cataaccgaa
     3301 gaagcttttc gggaacacgc cgtcacgacc cattacgtac atcagacgcg ctacgcctgc
     3361 gtgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg cgccaacctg
     3421 gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa
     3481 gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc
     3541 gccaatcagc gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc
     3601 ttccgacaga ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc
     3661 ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgcgtgc tcgccagcgt
     3721 accagcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag
     3781 cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag
     3841 gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg gaatggaagg
     3901 caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata
     3961 gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc
     4021 gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc
     4081 cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag
     4141 gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg ctagacccat
     4201 cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt
     4261 aacgttatgc gacatggtta ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt
     4321 tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta aatatctatc
     4381 cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa taaccgacgc
     4441 taattaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct aaatgggggg
     4501 gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg ctgattttcc
     4561 gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt catctcgcca
     4621 ccagccgctg gcaagccctg cggtctgttc attccagcca atccagacag gttcaaagag
     4681 agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac gttgcgccag
     4741 ataccgtggt aaatagccgc agccaaggcc gctaatttgc agctccagtt tggttttaaa
     4801 atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag aagcgcctgc
     4861 ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac ggtttaacgg
     4921 ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt caagatcgcc
     4981 caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc caacgataat
     5041 atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat tgataaactt
     5101 caggcgcgtc acgctatgat gttgataaaa agcttcaatg agaggcgcaa gcagagaaaa
     5161 aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat gcagtttgat
     5221 cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccctttct ctaataacat
     5281 ttttccggtg cgggtgaatt tagcgcggtg tccgctacga tcaagcaatt gaatatttag
     5341 gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt agagtttggc
     5401 tgacgccgcc gcaaaactgc cctctttttc tatggcatcg aggatcatca acacatccag
     5461 cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg tgatcgttta
     5521 ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt cattacaaat
     5581 ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga actgcggagg
     5641 ttgcagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg
     5701 cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag tgatagcgcc
     5761 aataacatct tctaaatgga ctaaattaac accatgttca ccatcagggg cggtttttcc
     5821 ggcaaagaag cgtcccggat gacgtcccgg tccgaccagg cccgcaagac gcagaatatc
     5881 gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata ctcgtccact
     5941 gttggttacc ggattacgcg gggtagtttc tttaaccgtg ccttgcgcgt cgccatagac
     6001 agatgttgag ctggtaaaaa taattcgggg aatacgatgg gccagcgcgc tatccactaa
     6061 ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg ccggaagcgt
     6121 aatgaccagc gcatcggcat ccataagggc atccagatcg tcagaatcgc aaactaactc
     6181 aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt cgacaccatc
     6241 ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg
     6301 catacctaac caccctaacc cgacaattgc gacctttttc atccgttatc tcctgataca
     6361 ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct atgaatgaat
     6421 ctgttcaata atgataacga catgctgcaa tgagcagtga atcatcagta accgcgtata
     6481 ccagacggtg ctcctctgta atgcgtcggg accagaaacc tgacaaatta tgtttcaggg
     6541 gttctggttt ccccttacct tcaaatggtg ttctgcgggt atctttgata agttcattga
     6601 tctttttaac aattcgctta tctgtttcct gccagtacag ataatcatcc catgattcct
     6661 cagaccagat
//
LOCUS       DAJTRQ010000175         6304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000175 DAJTRQ010000000
VERSION     DAJTRQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            70..375
                     /locus_tag="OO332_003791"
     CDS             70..375
                     /locus_tag="OO332_003791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944074.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(451..915)
                     /locus_tag="OO332_003792"
     CDS             complement(451..915)
                     /locus_tag="OO332_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5944075.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(912..1787)
                     /gene="nanK"
                     /locus_tag="OO332_003793"
     CDS             complement(912..1787)
                     /gene="nanK"
                     /locus_tag="OO332_003793"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5944076.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1784..2473)
                     /locus_tag="OO332_003794"
     CDS             complement(1784..2473)
                     /locus_tag="OO332_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5944077.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2521..4011)
                     /gene="nanT"
                     /locus_tag="OO332_003795"
     CDS             complement(2521..4011)
                     /gene="nanT"
                     /locus_tag="OO332_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5944078.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4121..5014)
                     /gene="nanA"
                     /locus_tag="OO332_003796"
     CDS             complement(4121..5014)
                     /gene="nanA"
                     /locus_tag="OO332_003796"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5944079.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5136..5927)
                     /gene="nanR"
                     /locus_tag="OO332_003797"
     CDS             complement(5136..5927)
                     /gene="nanR"
                     /locus_tag="OO332_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5944080.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1605 a         1705 c         1560 g         1434 t
ORIGIN      
        1 atcagatggc tgcttaaaaa aagaacaaat ggtagtgtcc gctattgcca gtacacctca
       61 aaagttgcca tgatttacat tgaacagctt gaattaatcc ataaaagtgg tgatgtgttg
      121 tatccagtaa aaataaccag aaaatcatca ggaaaaactg ccttccacct tgttccattc
      181 ggcttaaata agactcacga ccttctagag gtggaagatg cttcagaagc aattcggctg
      241 gtgattgatg aacgtcattc cattcgctgc agtacactta cagcaactat tacgaataaa
      301 aagggcaagc gaattaagcg gacaggtata tacagtatca agggtgttaa cataaaagaa
      361 tacaacgtca ggtaatggca cagagcggtc cgtcagttca ggtttggctc tgtgccaaag
      421 atgggtcagg tcacacctga tctaataaat ttaagccatt aaatcagcct taaccttcac
      481 caccaccttt ttaatctcac ccggctcgcc aacaacgcat cccggcttat gcggctctcc
      541 tggcataaac acggcgaaca ttcccggttt caggatgatg gtttgctcgt tttcaatggc
      601 gctacaaagc tggtaatcat cttcatggtg gaactcttca cattggcgcg cagtgcctgc
      661 catgccaaac agaatccgtt cctcaccgtt taataacagc tggatatcaa tgtattgctc
      721 gtgcaattcc gcttttttct cgacgggcga ttgagtgtta aacgtcatga cattcataaa
      781 aatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa
      841 ggccagcgtt aacgcgtcct gtaacgcagg atgtaaccca gcagacggta atgactgtac
      901 ttcacccatc atcataattt ttctccctgg gccaacagcg cagccccaag taaacctgca
      961 tcatggcggt aatgcgccgc cagtaaatca acatgaaatg ccgctggctc ctgcgccaga
     1021 tacatttcca ccaacgccag atacccttct gccagaccaa cgctgccacc aaccaccacg
     1081 cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag
     1141 cgatgaatca gttgctgcgc ctgctcatcg ccctgcccgg cgcgtgtgaa aatagttctc
     1201 gcatccgcgc cagccagctc gccctgcgct gccgccgcaa tgccgcgacc agaagcaatt
     1261 gcttccacgc aacctgtgcg tccacagccg cagactgggc cgtgtggatc ggcaagcgta
     1321 tgcccgatgt gccccgccag accgccaggg ccggtaagca gtttgccgcc gctcactaca
     1381 ccgccgccga cgccagtgga aacggtgata aagaccatat cggttatatc gccttccagc
     1441 gcctgatact ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt cggcaaattg
     1501 gtaagttgtt ccagcgtttt gactaacgga aagtgtagca atccaccgag attatgcgga
     1561 ttcagcgcca gcaaactgcc gtcgcgaata attccggttg aagcgatggc aacccgctgc
     1621 gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcaaggc ttctggtgtc
     1681 tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc gccaatcagc
     1741 gcggcggcaa gtttagtacc gccgatatca atcgccagta tggtcatagc accgcctttt
     1801 tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg
     1861 tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc
     1921 cttcggcaat cacccgacat ccggcttcgc tcaacgtttt caccagcgcc agatccggct
     1981 cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt tcggctccca
     2041 gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt
     2101 gatgatggat acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt
     2161 caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc gacatcttca atataggccg
     2221 tgatgcgtac cggagaatcc tccagatcgc gtttcacaat tccaataatc ggcacgctca
     2281 ccaccgcacg cgtggcttgc agatttgcca caccttcgat gcgaatggca accgcgcccg
     2341 cctgctctgc cgctaatgcc atggctgcga cgatttcggg tttatcgagc gggctgtcag
     2401 gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga tccagttgtg
     2461 caagtaacga catacatctt cccttagcga aaggcccggt acatagaccg ggcaacagga
     2521 ttaacttttg gttttgacta aatcgttttt ggcgctgcca aatggcacgg caccgctaaa
     2581 tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc aacgctgaac
     2641 gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcaggc tgaacgagag
     2701 cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga tgattggggc
     2761 cagtgcgcca cccaatgcgc caacgttgta ggtaaagccc aggcccgctg cacgctggtc
     2821 ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt gtccaagcat
     2881 ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac
     2941 cggaataatc agcagctgcg aggccagcag gctacaaacg tacgctttgt gggttcccag
     3001 ccagtcaccg aggaagccac ctacgcagca tcccacggcc gcgccaaagc cactaaagaa
     3061 cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat acgttggcag
     3121 cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca tcagcattac
     3181 gcccgttggc cagcgtttac ccgtactttg caccataaag ctgataaaga ttgcggcgca
     3241 taacagcccg agaacagcga cgattgcagc attttgcagg ttaccggcga agcagaacca
     3301 cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt caccacggta
     3361 gagaatatcc accattgtgc gtactggtgc tttacctgcg tgtttctctt tccagtcttc
     3421 cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa tgccgataaa
     3481 gaacagcgca cgccaacccc agaccggaac caccaggcta tagacctgag cggcaacgac
     3541 ggcccccaca gagaagcctg aaatcaaaaa accactggct ttgttacgca gatgttttgg
     3601 ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc ccatgccgat
     3661 gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg ccagcgtccc
     3721 ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc ggtcacccat
     3781 agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga tcagacttgc
     3841 cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca gggcgattaa
     3901 aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg cgcgccattg
     3961 tgcacggttg agatggcgat accacgggat attctgggtt gtagtactca ttgtgagtct
     4021 cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca gatgcgttta
     4081 tttcccctca cccggtaggg gcgagcgagg ggaaaacaac tcacccgcgc tcttgcatca
     4141 actgctgggc cagcgccttc agttctggca gatatttttc atctaccggt ccaaacggtt
     4201 tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc aggccgcgga
     4261 atacgcccgt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc agtttctgcg
     4321 cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgatatcgc cagcccatga
     4381 tgttgtaagt actgccgata ccaccatcag cgcctgccag caagccggaa gcgaagattt
     4441 cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgt tccatctgat
     4501 agagatcgcc agaagtctgt ttcagcgcac cgacgccagg caacgtaacc agggtgttga
     4561 tctgatccag ggtcagtttt accccactca gggctggaat gttgtacacc accatcggca
     4621 aaccatccgc tgaatcaata attgcccgat agtgatcgca gtgttcttca aagctgaaag
     4681 gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta gccgatgccg
     4741 caagttgttg gctttcagcg gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac
     4801 ctttcgcctc ttcggcgacg atttccagta cctgttcacg ctcggaaagg ctttgtacaa
     4861 aggcttcgcc ggtcgaacca cccacgtata aaccgtcgat gccctgctga atattgaact
     4921 gaaccaggcg gcgcagactc gctttatcca gtgcttgttg ttgatcaaaa ggagtcagga
     4981 gtgcagccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg atgcttgtct
     5041 gataaacgat atacctttat acctgttata ccagatcaat taagcaacac cccatacaga
     5101 aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt ggtggtctga
     5161 ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc acgatcggct
     5221 tcgtcaggat catgacggcg gatcgcatca acgatcgcaa tatgctgttg ataactaacg
     5281 ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc gtgcggcaat aagccagtcg
     5341 agcagggcaa cgtggatcgc catgaagatt gggttaccgg ggatctccgc cagcacgcgg
     5401 tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat ccagcgactg actgttgatt
     5461 tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc cgcatagcgc
     5521 accaggctgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc accaggatga
     5581 gaaaggaaat ctttcgccat gccggaaagc tcgccgatga tggtgtccgc agaaggtcga
     5641 gagacacgag cgcgttcgcc gttgtttatt tgcaccagac ctttgcgttt taacgctgcc
     5701 agcgcttcac gcacagaagg acgcccgacg ttaaagaacg ccatcagttc gcgttcagac
     5761 ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag ctcttcttcc
     5821 accatttcgg agagtttttt acgcgccagc gggcggctac gcaagttgcg accaattgca
     5881 ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa ggcccattct gtaaggtcag
     5941 tgtgattaac atcatcagtg acatcctatc acaggattga aagtagggga aaatggcagg
     6001 tttttctctt tgtacctcat cattaccata attaacggaa taattaacta ttgcgaaaaa
     6061 ttaatgtaac gcagataaac acatcccgat tgaattattc ataagactat tcacaagcat
     6121 tatgaatatt atgaatgtgt tcttacaaaa taatcataat cgcatatttt ttaatgaaaa
     6181 atcacctcac ctacaattaa aaacacgaca tccgcaccat aaatagactt gcaaaaaata
     6241 taacatcatt gttttcaatc tgccatttat gggattgacc gttttctttt gacacggagt
     6301 tcaa
//
LOCUS       DAJTRQ010000176         6231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000176 DAJTRQ010000000
VERSION     DAJTRQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            22..447
                     /locus_tag="OO332_003798"
     CDS             22..447
                     /locus_tag="OO332_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5944081.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKKTVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELSGRGR"
     gene            444..734
                     /gene="tnpB"
                     /locus_tag="OO332_003799"
     CDS             444..734
                     /gene="tnpB"
                     /locus_tag="OO332_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5944082.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
     gene            complement(986..1729)
                     /locus_tag="OO332_003800"
     CDS             complement(986..1729)
                     /locus_tag="OO332_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR5944083.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(1779..3146)
                     /locus_tag="OO332_003801"
     CDS             complement(1779..3146)
                     /locus_tag="OO332_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944084.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            complement(3218..4330)
                     /locus_tag="OO332_003802"
     CDS             complement(3218..4330)
                     /locus_tag="OO332_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HCR5944085.1"
                     /translation="MKITKLTTFIMHVPVTKNIIGDSTHSITHWGMPGVMIETDTGLV
                     GYGYTGTHADITTDRLITTIIKDAFGPMLIGEDASEVRYLHRKMSRSSTNIWVGRGGL
                     MQMALSAIDIALWDLKAKMVEQPLWKVFGGSQHSRVLAYNTDCGWLVRNTAELVDDCK
                     KMIFEEGFSAIKMKIGKPDPREDLKRIEAVRKAIGDDVNLMVDANAKWDISTAKQYGA
                     KLADFDITWFEEPMWHDDIASHRTLSGHISTPIALGELLYQFDSFKEFVLAGAVDYLQ
                     PDATRCGGLTAVWEIADLGQAFNLPVTPHHGDMMQAQLHLVMAHPSCSLLEFIPWTLG
                     CFVDPVEVKEGYYSLPTAPGAGTTLRPEALEHFSVR"
     gene            4496..4977
                     /locus_tag="OO332_003803"
                     /pseudo
     CDS             4496..4977
                     /locus_tag="OO332_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            5038..5471
                     /locus_tag="OO332_003804"
                     /pseudo
     CDS             5038..5471
                     /locus_tag="OO332_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5527..6231
                     /locus_tag="OO332_003805"
                     /pseudo
     CDS             5527..6231
                     /locus_tag="OO332_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    5807..5923
                     /locus_tag="OO332_003805"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1728 a         1496 c         1550 g         1457 t
ORIGIN      
        1 gatatagacg gacatctaag tatggaatta caggactggc gaaaagaacc tcgtaaaaac
       61 tattcgaatg aattcaaact tcgtatggtg gaactggcat cacaaccagg agcctctgtt
      121 gcccgtattg cccgggaaca cgatatcaat gataacctcc tgttcaaatg gctcaggctc
      181 tggcagaacg aagggcgtat atcacggcgt cttcaggtaa cgacctcttc tgacactggc
      241 gttgaattat tacctgtaga aataacgccg gatgagcaga aaaaaactgt ggcggccatt
      301 gcgccgtctt tatccacttc cactcagacc agagtcagtg ccagttcctg caaggtggag
      361 ttccgtcacg gtaacatgac gctggaaaat ccatcgccag agctgctcac ggtgttgatc
      421 cgcgaactga gcgggagggg aagatgattt cactcccatc aggtacccgt atctggctcg
      481 ttgccggcgt taccgatatg cgtaaatcct tcaacggact gggagaacag gtacaacatg
      541 tgctgaatga taatcccttc tccggccacc tgtttatctt ccgtggccga cggggtgaca
      601 ccgttaaaat tctttgggct gatgctgatg gtctgtgcct gttcaccaaa cgccttgagg
      661 aaggtcagtt tatctggcct gcggtgcgtg acggtaagat atccattacc cgctcgcaac
      721 tggcaatgct gtaaacgtta acggagcgcc gtattgacgc ttacgaactg tgtgatccga
      781 catgccatta ggaaacgtaa ggcgttcccg atgggcgagt cagtaaaagt gaaatggctg
      841 gcatcctggt cgcgtcgcag aaatggacaa tgccgcagag ggactggcga atagcaattt
      901 attatcgaat ttgatgaccg cctggacagt cacttttaag aaaaagcatg tatacagaat
      961 ggtaaacagg ctcatccaca gccaattact catcttcatc agcatataac tgcctgagtg
     1021 catttatatt gatgatcacg attttgccat atctggcgtc tatccatccc aatttttccc
     1081 attcaaccag ccgtttgttg atcgtttgtc ggctcgtatt gattatcgtt gccagatcgt
     1141 cctgcgtaag ataaagattg attaatgtcc ctcgctcgtg tggttcgccg tacccttccg
     1201 acagactcac taaacgcttc gccaagcgtg cagaaacaga taatagcgta cagtcatgta
     1261 acatcgtaaa ggcatttcga acgactttgc aaagcagcaa cgccagaaaa cgatggagaa
     1321 ttggataatt tttcagaaga aattcaacat catgaggctt gatcgttaac agtgtagttt
     1381 tacccgtcgc tctggcatcg tgtgtgcgcg gacatttatc aatcatacct atttcaccaa
     1441 accagttccc cggcgaaaga tagcggaaga tgcactcttt accgtcagca gaaattgagc
     1501 tcactttaac ggagccagta atgactgcat ataatccgag cggctcagca ccacgatagt
     1561 gaataatctc tccatcgttg tattgataca ggctggtcct ggacaacatc tgttcaataa
     1621 gataatcagg tagcgcccga aaccaactac tactaagaag gatactattg atctcgtcca
     1681 tagaaactat cgattcatgt gattgtgcta cggccaatat cttaaccatt gttgttatcc
     1741 tgatcttaat catgaccgta gcaatgtagc atttcctgtc aatcagagtg atgcagatta
     1801 tccccgctgg cagggagatt cggagatgtg gatgatgtac ggggcgtttt ttcaatcccg
     1861 aagtgacgta atgtcagaat catcaggcta ccagtagctg ttaccattgc cagtaagtaa
     1921 agtcctgcct gtgcggaatt aaaagtatgt tccgcccaga cgcgaagatt cggcgctaca
     1981 aaccccccca gattgcctat cgagttaatt aacgcaatgc cggaagcagc tgctgcaccg
     2041 ccgagataag acgatggaaa tgtccagtac agtggctgca tcgccagatg tcctgcagcg
     2101 gcaacgcaca gcgcgacaat agcgaccagt ggaatactat cggcaaggcc agatacggca
     2161 atgcctattg caccggacat aaatgtgatt gatgcaacgt aacgacgttc ctgcagtttt
     2221 tccgatatac gtggtacgta atacactgcg atggcagcgc acaaaggtgg aatagcggtg
     2281 acgaagccaa ctaataaacc aacttttgtc cctattaagg tggcgatttg tgttggcaga
     2341 aagaagtaaa ttccataacc acatacctgc accgtaaacc agatcaggca taaatagagg
     2401 acgcgtacat ccagcagtgc ttgccatgcg ccttttggac catgtgcaag tttcacctgc
     2461 tcttccttat ttagagtctg gctaagcgcc gctttctctt gtggagttaa ccatttggct
     2521 ttctctgggt gatcgtcaag atagaagaac acccagatcc caacggcaga ggccagtaac
     2581 ccctcaacga ggaacatgag ttgccagcca tggagaccaa acgacagtac cgatccttca
     2641 agcgagagta gcgagccgga aagcggaccg ccgatgataa aagctagtgg ggcaccaaac
     2701 aggaaatagc cggtgacgcg tgcgcggatg tgctggggga accagaatgt aagataaaga
     2761 ataactccag gaaagaaacc tgcttcactg atgccaagca agaagcgcag gaaaataaat
     2821 acggtctcag tgtgggcgaa catcattgca gcggaaacca gcccccaggt caccatgata
     2881 cgggccagcc agacacgggc gccgatgcga taaagcagaa tattactggg cacctcgaac
     2941 agggcataac caataaagaa gatcccggcg ccgaaagcat aggcggtgtc actcagactg
     3001 gtatccagtt gaaattcttg tttagcaaag ccgatattgg agcgatccag ataggccaga
     3061 ataaacatta gtatgaccat cggaatgatg cgacgtatgg atttttgaat tgcaagtgat
     3121 aaatactgat cggtatggct gggcataaat cacctcttag aattttgtca ttattatttt
     3181 gccccggtca ttgcataacc gggcgataca agtgttctca tctaacgcta aagtgctcaa
     3241 gagcttccgg gcgtaaggta gtgccggcac ccggcgcggt gggaagagag taatatccct
     3301 ctttgacctc aaccggatcg acaaagcagc ccagcgtcca gggaataaac tccaacagag
     3361 agcaagaggg atgtgccatt accagatgta attgtgcctg catcatatca ccatgatgtg
     3421 gtgtgaccgg cagattgaac gcctgaccga gatcggctat ttcccatacg gcggtcagtc
     3481 caccgcagcg agtggcatcg ggctggagat aatccaccgc accggccagt acaaactctt
     3541 taaaggaatc aaactgatag agtaactcac ccaacgcaat cggcgtactg atatgcccgg
     3601 acagcgtgcg gtgacttgcg atatcatcat gccacatcgg ctcttcaaac caggtaatat
     3661 cgaaatcagc cagcttggca ccgtactgct tggcagtgct gatgtcccat tttgcattcg
     3721 cgtcgaccat cagattgacg tcatcgccta tcgcttttct gacggcttcg attcgtttga
     3781 gatcttctcg cggatcgggc ttgcctatct tcatcttgat ggcggaaaat ccctcctcaa
     3841 aaatcatctt cttgcagtca tccactaact ccgccgtgtt acgaaccagc cagccgcagt
     3901 cggtgttgta ggcaagaaca cgggaatgtt gagaaccgcc aaagactttc cacaaaggtt
     3961 gctccaccat tttggctttc aggtcccaca gggcgatatc gattgctgaa agcgccattt
     4021 gcattaaccc accgcggccc acccagatat tggtcgagct gcgtgacatc ttacggtgga
     4081 gatagcgaac ttcactggcg tcttctccaa tcaacatcgg gccgaaggca tctttaataa
     4141 tggtggtgat aagacggtcg gtagtaatgt ctgcatgggt tccggtataa ccgtaaccta
     4201 caaggccggt atctgtctcg atcattacgc ccggcattcc ccaatgagta atgctgtgtg
     4261 tggagtcacc gataatattt ttagttaccg gtacatgcat aataaaggta gtgagtttag
     4321 tgattttcat tgcagtttcc tgtgtctgta tatcttgatt gatgggcgca atgtagcaac
     4381 cggccttcag cagggctgtc aaatggttga cacatcatca gaagagagaa ggaataattg
     4441 tatttttttg aagtagatct cgaatttgga ccacacccag tcgacaagtt ttcatatagg
     4501 gaactgggcc atgaccggag cttttcagga tgagatctct gtacgcatca tgaagaccat
     4561 gcggctaaag tgatcctgga gtacagcaaa ctggaaaaaa tcgaaaagta ttacgaaaaa
     4621 tacgcaaacg caggcaccaa tgagtggcac atcgatgaaa gtaaaaaagc taccgcaacc
     4681 atgaatgtag ttctgggtga tgaagatagg ttaaaatccc tcgcggaaga ttttgccaga
     4741 cattatgaaa aacgcgtagc cgaaggtttc accgtaaaag gcaagaccat gtttgtgcca
     4801 gccgtgaaat tgcctagggt tctaccgtca ccttgaagct attcgcactg cctggtttta
     4861 agtgaaacaa gtacccgatg gcatcgtcct gactgaacgg gagcagaaaa attaccgcct
     4921 tctgaaatgg tgaaaatggt catgacgcgt ggtaaagatg atgacgaggc gctttataga
     4981 tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg ttaatttatg
     5041 gacgaaaaat agttacagac tctggctaac gaactggcca aaaacctcaa aacccctgaa
     5101 gacctcagcc agtttgaccg tctactgaaa aagatcagcg ttgaagccgc tctcaacgca
     5161 gagatgacac accatcctgg gtatggaaaa atcagtccag accaggagct aactcccgca
     5221 acggttattc cacaaagacc gttatcacag gcgacggtcc actggaactg cgtactccgc
     5281 gcgatcgtga cggtagcttc gaaccacaac aggtgaagaa aaatcagccc cgtattacca
     5341 ggatggataa ccagatcctc tcgttgtatg ccaaagggat aaccacccgt gagatagccg
     5401 ccgcgttcaa agacctgtat gacgctgatg tttcaccggc actgatatca aaggtcactg
     5461 atgccgtgat ggtgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5521 aacaggatga ctaaaaatac acgttttccc ccgaaatccg tcaacgggca gttcgtatgg
     5581 ttctggaaag tcagggcgaa tatgactcac aatgggcagc aatttgctcc attgctccaa
     5641 agactggctg tacgccggag actctgcgtg tctgggttcg ccagtatgag cgggataccg
     5701 ggggcggaga tggagggctc accagtgctg aacgtcagcg tctgaaagag ctggaacgtg
     5761 aaaatcgtga actgcgccgc agcaacaata tccttcgcca ggcttccgct tattttgtga
     5821 aggaggagtt cgaccgcctc tggaaaaaat gatgccactg ctggataagc tgcgtgagca
     5881 gtacggagtc agaccggtat gcagcgaact gcatattgcc ccgtcaacgt attaccattg
     5941 tcagcaacag cgacatcatc cggataaacg cagtgcccgt gcgcagcacg atgactggct
     6001 gaagaaagag atacagcgcg tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt
     6061 ctggcgtcag ttgttacggg aaggaatcag ggtggccaga tgtacagtgg cgcgcctcat
     6121 ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg
     6181 gaaaaccgtt gccacaggtg accgcgtaaa ccgtcagttc gtggcagaac g
//
LOCUS       DAJTRQ010000177         6105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000177 DAJTRQ010000000
VERSION     DAJTRQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO332_003806"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO332_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5944086.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="OO332_003807"
     CDS             962..1303
                     /locus_tag="OO332_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5944087.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="OO332_003808"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="OO332_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="OO332_003809"
     CDS             complement(1901..2611)
                     /locus_tag="OO332_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5944088.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="OO332_003810"
     CDS             2719..3024
                     /locus_tag="OO332_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5944089.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="OO332_003811"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="OO332_003811"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="OO332_003812"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="OO332_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5293..>6105)
                     /locus_tag="OO332_003813"
     CDS             complement(5293..>6105)
                     /locus_tag="OO332_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944090.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVTV
                     PSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMVD
                     VLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKAL
                     SRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITVV
                     LSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
BASE COUNT         1580 a         1553 c         1498 g         1474 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggctattg cgggtaggca ggacttctac agggaacgga atgtt
//
LOCUS       DAJTRQ010000178         6069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000178 DAJTRQ010000000
VERSION     DAJTRQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(127..276)
                     /locus_tag="OO332_003814"
     CDS             complement(127..276)
                     /locus_tag="OO332_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944091.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO332_003815"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO332_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5944092.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO332_003816"
     CDS             2367..2669
                     /locus_tag="OO332_003816"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944093.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OO332_003817"
     CDS             complement(2845..4338)
                     /locus_tag="OO332_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR5944094.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OO332_003818"
     CDS             complement(4382..4771)
                     /locus_tag="OO332_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944095.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OO332_003819"
     CDS             4989..5429
                     /locus_tag="OO332_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5944096.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1447 a         1664 c         1592 g         1366 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg cattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctagaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 taagtgatgt aaaagtgatg tataagtgat gtaaaagtga tgtatgaact gaaggagcga
     5881 tgtaattctg tagtcaggaa gaataacccg ttcatgcagg catgaacgat acagtgagga
     5941 acgctttttt tgtcacgatc tccccttaac ctgcgtaaat cacccgccat ggttgattat
     6001 ttttcaacct caaaatggtc atacacctaa cgcagccgcg attttcatcg caactgcatg
     6061 aattaaaag
//
LOCUS       DAJTRQ010000179         6033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000179 DAJTRQ010000000
VERSION     DAJTRQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            171..818
                     /gene="fucA"
                     /locus_tag="OO332_003820"
     CDS             171..818
                     /gene="fucA"
                     /locus_tag="OO332_003820"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5944097.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            846..1994
                     /gene="fucO"
                     /locus_tag="OO332_003821"
     CDS             846..1994
                     /gene="fucO"
                     /locus_tag="OO332_003821"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5944098.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2047..2802)
                     /gene="xni"
                     /locus_tag="OO332_003822"
     CDS             complement(2047..2802)
                     /gene="xni"
                     /locus_tag="OO332_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5944099.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="OO332_003823"
     CDS             complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="OO332_003823"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5944100.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4338..5627)
                     /gene="sdaC"
                     /locus_tag="OO332_003824"
     CDS             complement(4338..5627)
                     /gene="sdaC"
                     /locus_tag="OO332_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5944101.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1517 a         1486 c         1613 g         1417 t
ORIGIN      
        1 agagaacttg cagttattta ctgtgattag ttgagctagg tcacaaaata taagcttgtg
       61 aatttctttc ttgttaatca ggaagaagat ggtgggtaat taaacggcta attcaatagt
      121 gtgaaaggaa caacattatt gccctgtttt gaatcagaga gaggtagcta atggaacgaa
      181 ataaacttgc tcgtcagatt attgacactt gcctggaaat gacccgcctg ggactgaacc
      241 aggggacagc ggggaacgtc agtgtacgtt atcaggatgg gatgctgatt acgcctacag
      301 gcattccata tgaaaaactg acggagtcgc atattgtctt tattgatggc aacggtaaac
      361 atgaggaagg aaagctcccc tcaagcgaat ggcgtttcca tatggcagcc tatcaaagca
      421 gaccggatgc caacgcggtt gttcacaatc atgccgttca ttgcacggca gtttccattc
      481 ttaaccgatc gatccccgct attcactaca tgattgcggc ggctggcggt aattctattc
      541 cttgcgcgcc ttatgcgacc tttggaacac gcgaactttc tgaacatgtt gcgctggctc
      601 tcaaaaatcg taaggcaact ttgttacaac atcatgggct tatcgcttgt gaggtgaatc
      661 tggaaaaagc gttatggctg gcgcatgaag ttgaagtgct ggcgcaactt tacctgacga
      721 ccctggcgat tacggacccg gtgccagtgc tgagcgatga agagattgcc gtagtgctgg
      781 agaaattcaa aacctatggg ttacggattg aagagtaatt tcgtaaagca acaaggagaa
      841 ggatgatggc taacagaatg attctgaacg aaacggcatg gtttggtcgg ggtgctgttg
      901 gggctttaac cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc gtcaccgata
      961 aaacgctggt gcaatgcggc gtggtggcga aagtgaccga taagatggat gctgcagggc
     1021 tggcatgggc gatttacgac ggcgtagtgc ccaacccaac aattactgtc gtcaaagaag
     1081 ggctcggtgt attccagaat agcggcgcgg attacctgat cgctattggt ggtggttctc
     1141 cacaggatac ttgtaaagca attggcatta tcagcaacaa cccggagttt gccgatgtgc
     1201 gtagcctgga agggctttcc ccgaccaata aacccagtgt accgattctg gcaatcccca
     1261 ccacagcagg tactgcggca gaagtgacca ttaactacgt gatcactgac gaagaaaaac
     1321 ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt attgatgctg
     1381 acatgatgga tggtatgcct ccggcgctga aagccgcgac gggtgtcgat gcgctcactc
     1441 atgctattga ggggtatatt acccgtggcg cgtgggcact aaccgatgca ctgcacatta
     1501 aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat aaggatgccg
     1561 gagaagaaat ggcgctcggg cagtatgttg cgggtatggg cttctcgaat gttgggttag
     1621 ggttggtgca tggtatggcg catccactgg gcgcgtttta taacactcca cacggtgttg
     1681 cgaacgccat cctgttaccg catgtcatgc gctataacgc tgactttacc ggtgagaagt
     1741 accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg gaaggggcgc
     1801 gtaatgccgc tgttgaagcg gtgtttgccc tcaaccgtga tgtcggtatt ccgccacatt
     1861 tgcgtgatgt tggtgtacgc aaggaagaca tttcggcact ggctcaggcg gcactggatg
     1921 atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta gagctttacc
     1981 ataccgcctg gtaaatgcgc tgatgtgata atgccggata cgatttgcgg cgtatccggc
     2041 tcgccgttac cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg
     2101 taagcgggca atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg
     2161 ccacttttcg gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac
     2221 cagcagctgc gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct
     2281 gatccccgca agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc
     2341 gataaatggc gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga
     2401 aagtaactga cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggccagtgt
     2461 cactttgacc gccagtgtgg cggctaagtc atcggcttcg ttgccgctgg ctgaccagca
     2521 cgggacgccg cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc
     2581 cggcattggc ggtcgatccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact
     2641 gcggttttca tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg
     2701 atcgagcgca tgctggcagg tttcgacaca gggcgaaccc tgaacggcat gaatgcgacg
     2761 gataagattc agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat
     2821 caggcgataa gggtaacatt cgatgccgga acaggctatg gctggttgag aaaacaggaa
     2881 gaggcttcgc aaaacgaggc ctctgggaga gattaatcgc aggcaacgat cttcatcgcc
     2941 aggccgccgc gagaggtttc gcggtacttg gcgttcatgt ctttacctgt ttcgtacatg
     3001 gtttcgataa ctttatcgag gcagacgcgc ggttcgctgg tacggcgcag tgccatacgc
     3061 gcggcgttca ccgctttcac tgctgcaatg gcgttacgct caatgcacgg tacctgaacc
     3121 tgccccgcga ccgggtcaca agtcagaccg aggttgtgct ccatcgcgat ttccgctgcg
     3181 atgcaaacct gcgccgggct tgcgcctaac agttctgcca gaccagctgc cgccatagag
     3241 cacgccacgc caacttcacc ctggcaaccc acttcggcac cagaaatcga cgcgttcatc
     3301 ttataaagag aaccaatggc gctggctacc agcaggtaac gagccagtga gttagcgttc
     3361 acttcgcgga taaacttatc gtagtacgcc agcacagccg ggataatccc gcacgcaccg
     3421 ttagtcggcg cagtcaccac gcgaccgcca gcagcgttct cttcgttcac tgccagtgca
     3481 aacatgttga tccagtcaac aaccgccatc gggtcagtgg tggttttatc ctggctgacc
     3541 agcatccggc gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg caacacgcct
     3601 tcggtggaaa taccgcgctc aataccgcca cgcatgactt cccagacgtt cgccaggtgc
     3661 tgttccagct cttctttact gtgcagcgcc agctcgtttt tcatcatcag gccagagagt
     3721 gacagcccgg tttcctgaca atgtttttgc agatcggctg ctgaactgta cggataagga
     3781 acttcaaccg gtgcgctatc ctgctggcca aaatgctctt catcaacgat aaagccaccg
     3841 ccaatagagt agtaagtctg gctgtaaacg actttatcgc ccgccagcgc ggtaatgcgc
     3901 ataccgtttt catgcagaga aaggttgtct gcatggaagt tcatgcactg atcaaccggg
     3961 aactccactt catgctgacc gtttgccagc atcaggcgac catgagtatt cacatcctga
     4021 ataaaaccgg ggatggaatc gatatccacg gtatccggca ggttacccgc caggcccata
     4081 ataatggaga tatctgtgtg gtggccttta ccagtcagag agagcgagcc gtacacgtca
     4141 accaccacgc gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc ggtaaattgt
     4201 ttacccgctt tcattggtcc aacggtatga gaactggaag ggccaatgcc gattttgaaa
     4261 atatcgaata cgctaatcat aggaaataca tcgcgttaaa acggaggaag cgccgcccga
     4321 aagcggcgcg aaaggactta gctgaacaga gagtagaaga ttgcggagat tgcaatcagc
     4381 cccatcacga caacgaatac gttgctgatg tgaccgctgt acttacgcat tgccggtact
     4441 ttctgaattg cgtacatcgg catcaggaac aggatcatcg cgatgattgg gccgcccagg
     4501 gtttcaatca tacccaggat gctcgggttc agggtggcaa caatccaggt cgttaccagc
     4561 atgaacagcg cagtgatacg gttcagcttg ttgatttcga tagacttacc tttaccacgc
     4621 agagatttaa tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg gccgaggaag
     4681 gatttggtga tagcgataat cgcgataatc ggagccatcc acgcgataac cggtgcgtta
     4741 aagtggttag ccaggtaaga cagaatcgag atgttctgct ctttagccgc agccaggtct
     4801 gccggagtca ggctcagtac gcagctgaag acgaagaaca ttacggtcag caccatcatg
     4861 atgtgtgcga atgccaggat cttcgagcat ttctgttctg ccatatcgcc gtactcttca
     4921 cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt tgaacgagaa caccattacc
     4981 ggaattgcca gccacagggt catccacaga ccgtttccgg ttgcagatgc agtgtccaga
     5041 gacagcgttt ccagtgctgc gccgttccac tgcgggatca ggtacagagc cagcagcatc
     5101 agtacgccaa caaacgggaa taccagaata ctcatcgctt taacgatcat ctgctcacca
     5161 aagcgaacga tggtcatcat acccacgatc aggatcagcg acagaatcgc acgcggcggt
     5221 ggcgtcatac ccagctggtg agacatgaag ctttcaacgg tattggtgat tgccacgcta
     5281 taaaccagca ggatcgggta gatagcgaag aagtagagca gggtaatcag tttacctgcg
     5341 ccaataccaa agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt tttaccagac
     5401 agtacgaagc gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc
     5461 atgatgatca gcgggatcat accgccaaca ccggcgttga ttggcaggaa cagcacgccc
     5521 gcgccgattg ccgtgccgta aaggcccagc atccacatgg tgtctgtctt gcgccaggca
     5581 ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta tttctcctgg
     5641 aggaatgcaa ataattcagc ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa
     5701 atgaaatctg tctatcggcg atggcttaat catttaccgc tgtaataatt tgagggcgga
     5761 aagatacaca caagttatgt atctatcagt gatcttgatc ccaaatacaa taatctgctt
     5821 ggaagataga ttatcccaac gctttactct gtaaatcaaa agtaaaagag aatttaaggg
     5881 caatatttgc ccattaaacg acatctgact tcttccgggg cgcgcaatat atgtaggttg
     5941 aactgaaaaa atcaatactt ttagttaatc atggcgataa tttctttttc atagcgataa
     6001 aatacggttt atgaaaatag ggaaacgttt gcg
//
LOCUS       DAJTRQ010000180         5961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000180 DAJTRQ010000000
VERSION     DAJTRQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.182"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..233)
                     /locus_tag="OO332_003825"
     CDS             complement(<1..233)
                     /locus_tag="OO332_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5944102.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGM"
     gene            770..2671
                     /gene="mtlA"
                     /locus_tag="OO332_003826"
     CDS             770..2671
                     /gene="mtlA"
                     /locus_tag="OO332_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5944103.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2766..3914
                     /gene="mtlD"
                     /locus_tag="OO332_003827"
     CDS             2766..3914
                     /gene="mtlD"
                     /locus_tag="OO332_003827"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5944104.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3914..4501
                     /gene="mtlR"
                     /locus_tag="OO332_003828"
     CDS             3914..4501
                     /gene="mtlR"
                     /locus_tag="OO332_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5944105.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4513..4722)
                     /locus_tag="OO332_003829"
     CDS             complement(4513..4722)
                     /locus_tag="OO332_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944106.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5006..5368
                     /locus_tag="OO332_003830"
     CDS             5006..5368
                     /locus_tag="OO332_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5944107.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1561 a         1442 c         1480 g         1478 t
ORIGIN      
        1 tgcatacccc agccacgctc cggttgatag agcgtcgccc agatccacag aaacggccag
       61 ataacatgca gagtaaacag gctcacccag cccgccgtat gaatagcgtc ggcatggggg
      121 tgattgcgct ttttggcaat cagataaggg atgtcatgta tcgcgatgat tccataaaaa
      181 atcaccagaa agacgaagat cagcactccc aacgcgaaat agtttagaaa catatctgcc
      241 tcggattcac gtttatcagt gttgtttttt ggctggcagc cagaagggag tcaggctgat
      301 attttgacaa taatccgggt tcgcgattct cgccataaca ccaaagaata atttttagag
      361 gtgatgagtt gcttagttac ataacgattg tatgacgaag gcataacatg ctgtagatca
      421 catcaggtga acgccgtaag aaaatatctt gtgattcaga tcacaaagat tcaacaaacc
      481 atcaaaacaa aaatgtgaca ttactcacat ttaaatgcca tttttagcga aaatcaccgc
      541 cttgtttctt ttttacacag gcgttttgtg atgagcgtca cgtcaattac ctctctaccc
      601 cctatattta tgtgactgat atcacacaaa agcccgtcga ctggacagtt aaccgattca
      661 gtgccagatt tcgcagtatc tacaaggtcc ggctacctct gccgccacat taacaaaaaa
      721 cctcgggctt ccagcctgcg cgacagcaaa cataagaagg ggtgttttta tgtcatccga
      781 tattaagatc aaagtgcaaa gctttggtcg tttcctcagc aacatggtga tgccaaatat
      841 cggcgcgttt atcgcgtggg gtatcatcac cgcgttattt attccaacag ggtggttacc
      901 gaacgagacg ctggcgaagc tggtcgggcc gatgatcact tatctcctac cgctgctgat
      961 cggttatacc ggtggtaagc tggtaggcgg caaacgtggc ggcgtagtcg gtgccattac
     1021 caccatgggc gttatcgtcg gcgcagacat gccgatgttc ctcggttcta tgattgcagg
     1081 tccgctgggc ggctggtgca ttaagcactt cgaccgctgg gtagacggta agatcaaatc
     1141 cggttttgag atgctggtga ataacttctc cgcaggcatc atcgggatga tcctcgctat
     1201 tctggcattc ctcggcattg gcccgattgt tgaagccctg tccaaaatgc tggctgcggg
     1261 cgttaacttc atggttgtcc atgacatgct gccgctggcg tctatctttg ttgaaccggc
     1321 gaaaatcctg ttcctcaaca acgccattaa ccacggtatc ttctcgccgc tgggtattca
     1381 gcagtcccat gaactgggta aatcaatctt cttcctgatt gaagctaacc caggtccagg
     1441 tatgggcgtg ctgctggcgt acatgttctt tggtcgtggt agcgctaaac agtctgcggg
     1501 cggtgcggca atcatccact tcctgggtgg tatccacgaa atctacttcc cgtatgtgct
     1561 gatgaatccg cgtctgatcc tcgcagtcat cctcggcggt atgactggcg tgttcacgct
     1621 gactatcctg ggcggtggtc tggtttctcc ggcatctccg ggttctatcc ttgctgtact
     1681 ggcgatgaca ccaaaaggtg cttatttcgc taacatcgcg ggtgtgtgtg cggcgatggc
     1741 tgtctccttc gttgtctctg ctattttgct gaaaaccagc aaagtgaaag aagaagatga
     1801 tattgaagca gcaactcgtc gtatgcagga catgaaagct gagtctaaag gcacatctcc
     1861 gctatctgcg ggcaatgtga ctaacgacct gagccacgta cgtaaaatca tcgttgcctg
     1921 tgacgccggt atgggttcaa gtgcgatggg cgcaggcgtg ctgcgtaaga aaattcagga
     1981 tgcaggtctg tcgcagattt cggtcactaa cagcgcgatc aacaacctgc cgccagatgt
     2041 ggacctcgtc atcactcacc gtgacctgac cgaacgcgct atgcgccagg ttccgcaggc
     2101 acagcatatt tcgctgacca acttcctcga cagcggcctg tacaccagcc taaccgaacg
     2161 tctcgttgct gcccagcgcc atactgaaaa cgaagtgaaa gtgaaagaca gctttgacga
     2221 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2281 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2341 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2401 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2461 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2521 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2581 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2641 agtgctggaa ctgctggcag gtcgtaagta atccaatcct accctctcct cacggagagg
     2701 gtttgggtga gggaaaagcc tcaccccagc cctctcgggt aaaaacattg atgaaggtta
     2761 atactatgaa agcattacat tttggcgcag gtaatatcgg tcgtggcttt atcggtaaac
     2821 tgctggcaga cgcgggtatc caactgacgt ttgccgatgt caatcaggtg gtacttgatg
     2881 ccctgaatgc ccgtcatagc tatcaggttc atgtggtcgg tgaaaccgag caggtagata
     2941 ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga tgtcgttgat ctgattgctc
     3001 aggtcgattt agtcactacc gccgttggcc cggttgtgct ggaacgtatt gctccggcta
     3061 tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga atccccgctg aacatcatcg
     3121 cctgtgaaaa catggtacgc ggcaccacgc agctgaaagg tcatgtgatg aacgccctgc
     3181 cggaagacgc caaagcgtgg gtagaagagc acgttggctt tgtcgattcc gccgttgacc
     3241 gcatcgtacc gccttcggct tcggcaacta acgatccgct ggaagtgacg gtagaaactt
     3301 tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc actgccgaac atcccaggca
     3361 tggagttaac cgacaacctg atggcatttg tcgaacgtaa actcttcacc ctgaacacgg
     3421 gtcatgctat aaccgcgtac ctcggaaaac tggccggtca tcagaccatt cgtgacgcta
     3481 ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat ggaagaaagt ggtgcggtac
     3541 tgatcaagcg ctacggcttt gacgcagaca aacatgcggc gtacatccag aaaatcctcg
     3601 gtcgttttga gaacccgtat ctgaaagatg atgtagagcg cgtaggccgt cagccgctgc
     3661 gtaaactgag tgctggcgac cgtctgatca agccactgct cggtacgctg gaatacagcc
     3721 ttccgcacaa gaatctgatt caggggattg ctggtgcaat gcacttccgc agtgaagatg
     3781 atccgcaggc tcaggaactg gcagcactga tcgctgacaa aggtccgcag gcggcgctgg
     3841 cacagatttc cggtcttgat gccaacagcg aggttgtatc cgaggcggta accgcttata
     3901 aagcaatgca ataatggtgg accaggcgca ggacaccctg cgcccgaata acagattgtc
     3961 agatatgcag gcaacaatgg aacaaaccca ggcctttgaa aaccgtgtgc ttgagcgtct
     4021 gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc gtcgagcttt tgaccgaggc
     4081 ggtaaatctt ctggtgcttc aggtattccg caaagacgat tacgcggtga agtatgctgt
     4141 agaaccgtta ctcgacggcg acggtccgct gggcgatctt tctgtgcgtt taaaactcat
     4201 ttacgggttg ggcgtcatta accgccagga atacgaagat gcggaactgc tgatggcatt
     4261 gcgtgaagag ctaaatcacg acggcaacga gtacgccttt accgacgacg aaatccttgg
     4321 accctttggt gaactgcact gcgtggcggc gttaccaccg ccgccacagt ttgaaccagc
     4381 agactccagt ttgtatgcaa tgcaaattca gcgctatcaa caggctgtgc gatcaacaat
     4441 ggtcctttca ctgactgagc tgatttccaa aatcagctta aaaaaagcct ttcaaaagta
     4501 agcaacgtct gcttactgcc cctctacctg cttcggccga taaagccgac gataatactc
     4561 cagacgttgt agatataacg gcacgctttc atcgggtatt ccggacggaa tcgcgttacg
     4621 gggaggaagt tttttcagat actcccggaa cgcctggctt gatgccatga aatctacggc
     4681 tttatcgata agaagcggaa cgttttcacc taatttgccc atgatgatat ctccgtatta
     4741 ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg ttcccggtaa agatgacatt
     4801 aaggaaaacg tgctgaatcc tcaataatca tccgctgctg tttattatct ttttctatag
     4861 atttcttaat taatcaacga attgcgtagt ttttgagcat gttaatgatt acgttgcaga
     4921 actattacaa tgtcatcagt tgtgaacaaa gcacctggtc gcgcatacta ggggctataa
     4981 atttatcttt atcagaagcc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct
     5041 cagcgatcgc ctggacgcca tccgccacca gcaggccgat ctgtcgctgg ttgaagccgc
     5101 agacaaatat gccgagctgg aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct
     5161 gcgtgaggtt catagccaaa aactgagtaa agaagcacag aagctgatga agatgccgtt
     5221 ccagcgagca attaccaaaa aagagcaggc tgatatgggc aagctgaaga aaagtgttcg
     5281 cggactggtg gttgtgcacc caatgaccgc gctggggcgc gaaatgggcc tggaagagat
     5341 gactggattt tctaagacta cgttttaagc ccctcagacg actggttcga tcgtatcaac
     5401 ccgcatctgg cttgccttag ctggtcaatg cggggactct cctgtctact cgcctaatta
     5461 ccaaacaatt cactcccttt acttcgattt accgctggtt gatattctga aaatgcattt
     5521 ttaccgaaat agccatctat aaacaataaa aatatataga agatatcatt tgttaattaa
     5581 caagcattta cgtctctttt tttcggattt aattgtgaaa ttttaatcat catattcaca
     5641 aaatcaatta tctgtaaaat ttttcccaga catggtttct ttcatatttg acatttatta
     5701 ataatcatca ttagaatcca cgaaaaataa tatattcata caaacacaga catcttttga
     5761 aacgaatgat acatttttat gatgaatgct taaaacaata tttaatcgac acacatcact
     5821 gtcattaatg taaagcaaca tagctcacca ataatacaat ggacaactct accggcattg
     5881 atcgctggtc ggaggaaata aggattttta catgaacttg aagaagattt ttttcagtgc
     5941 tgtcactgtt tcagttctct g
//
LOCUS       DAJTRQ010000181         5957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000181 DAJTRQ010000000
VERSION     DAJTRQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1113
                     /locus_tag="OO332_003831"
     CDS             <1..1113
                     /locus_tag="OO332_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR5944108.1"
                     /translation="VWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAM
                     AVAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTD
                     QALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQR
                     DPFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARV
                     FIDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFI
                     DARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMH
                     DQPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1125..1937)
                     /locus_tag="OO332_003832"
     CDS             complement(1125..1937)
                     /locus_tag="OO332_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944109.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1941..2726)
                     /locus_tag="OO332_003833"
                     /pseudo
     CDS             complement(1941..2726)
                     /locus_tag="OO332_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2723..3391)
                     /locus_tag="OO332_003834"
     CDS             complement(2723..3391)
                     /locus_tag="OO332_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5944110.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3455..4093)
                     /locus_tag="OO332_003835"
                     /pseudo
     CDS             complement(3455..4093)
                     /locus_tag="OO332_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4106..5957)
                     /locus_tag="OO332_003836"
                     /pseudo
     CDS             complement(4106..5957)
                     /locus_tag="OO332_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
BASE COUNT         1511 a         1598 c         1370 g         1478 t
ORIGIN      
        1 gtctggcctg atgaagaaga agcaaccaga acgtattttc ccagcccgtg gtcaggcctg
       61 atggaagcaa aggtcgatgc gtgtaacgtg ggtattatgg gaacatcctt gagcggactg
      121 gatgcggcaa tggcagtggc tattcagcat ggttcgttca ttgaagatga taaacaacac
      181 gtcgtttttc accgcgataa cgcaagtgaa aagctaaata ttacgttgat gtcgcgcacg
      241 ggtattttac ccgaagccga tttctattgc cctattccct gcgagccctt acacatcgtc
      301 accgatcagg cattaaatgc tgagattcaa aaaggtgaag agggcctttt ggatcgggta
      361 tttagattga tagtagagga aatcaagttt gctgatccag actggagcca acgcatagcc
      421 ttagagagcc tgaatgtcga ttcctttgct caagcctggt ttgccgagcg caaacaacgc
      481 gacccatttg actgggcaga aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa
      541 catactgttc cctggcgtta tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt
      601 cctcatctga atgaacacga ccataaacgg ttcagtaaag gccttgccag ggttttcatc
      661 gataattatg cggcaatccc ttcagagtct attcgtcgac tactggcctt acgtgaagcg
      721 gggatcattc atattctcgc cctcggtgaa gactacgaaa tggaaattaa tgagtcgcgc
      781 accgtcctga aaacggaaga caacagctac tcgtttgacg tttttattga tgcccgcgga
      841 cagcgtccgc ttaaagtgaa agatattcct ttccctgggc tacgcgagca attacagaaa
      901 acaggggatg aaatccctga tgttggcgaa gattatacgt tacagcaacc cgaagatatt
      961 cgtggacgcg tagcgttcgg cgcgttgccc tggctgatgc acgaccagcc tttcgttcag
     1021 ggacttacgg catgtgcaga aattggtgag gcgatggctc gggcggtcgt aaagcctgca
     1081 tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa gctatcagca ctcaacaaac
     1141 tgatgctgtt cattaagacg atagcggact ccggttcgaa tattgttttc accttcaatg
     1201 gcgacggcga agcgaactct ttcgccgtca tgatatgcca gtgcggcgct tccccctagc
     1261 ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg ttgaattttc accgctggca
     1321 atgatgtgta cattgtcgcc gctgttggca attctggcgt ttgcgccaaa gctggcgatt
     1381 tgcaccaggt caccattact ggcgacatga caccgttcac ccaatgtaca aacgcgtact
     1441 cgcattccgg tattcgcaat ccgactacta tctccagcgc tgcttatccg cgaagaatta
     1501 ccggttacag caacccgtgc gcgttcaccg gagctgccaa tgtgactgtt aaagccgaca
     1561 cttccaatcc gcacgctata acccatactg gcaatttgcg cggcataccc agcggttgcg
     1621 attcgggcgt tatcctcaca acagccgatg cgagcatgat cgtcgctgcg ggcaaactga
     1681 ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt gcatagagga aacttcctga
     1741 tgcgcgaagt tttcatccgc cagccatttt ttccacgcat actcaaccag actctccgcc
     1801 cagtccgtat atccttcctc aatcagtgca tgacgaatat cggcataact accgccgcgt
     1861 gggaaatggc gcaggaacca gcgatacatc accgctccga tacgccattc tcgcaggtct
     1921 gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat cgaccatgct tttaaaggtt
     1981 tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac cgaagataaa aaagaggctt
     2041 ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc ttacgccagg aaacacatct
     2101 cccacggctt gcggatagag acacaacagc cccgtcaata gtaacaacgg cagcaatcca
     2161 tacatgacac caacataggc gacctgctgt aagggattaa atttagactg tgttgttgcc
     2221 gggaaaggat gttcttcccc ctgcataatg ccaaacaaat aaaatcgcgt ttgttttgcc
     2281 gctcgttcca gccacccctg acgacgaatg cgatagtggt gaccattatc cccaacggca
     2341 ttgatcagca caaagccgag ccagcatgcc agtaacaaaa atcagcaaac ttcatgcacc
     2401 gcaaccagac ttttaaccgc agttgcgccc accatcgcaa agtgatttat cagcccactt
     2461 gccagcaaca atacaaagag taacgcattc gaccagtgcc ataatctgac cgctttgctg
     2521 taaagataaa ccttctcacc atgatcggtg gctgatttct tcacgccacg agcacgaagc
     2581 aaggcgtgca gccccagcac caaccacatc ccaaccagca atactccggc aatgatcaac
     2641 cacaccggcc aaaattcggg agtgaactgt ggcacatagt tcgctaactg gctctgaaac
     2701 tgttcagcat gttgcgacgg gttcatacat tttccttttt aatcaaatgt tgaccgaacc
     2761 ggcgatacag atgcggtttt ccggcaccag gtagctgata ttgatagtat ttattatcct
     2821 gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa gatcaatgcg tgttccggac
     2881 aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac
     2941 atttatctgc cactttagtg acagggttaa ggtagcgaac ctggtacgga caagcgccaa
     3001 tgcaataact gcagccgatg cactgcgatt tttctacccg cacgatcccc tgctcatcac
     3061 gccacgatgc ccccgtcggg catacgtcga tgcacggagc atcttcgcag tgctgacatg
     3121 actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta
     3181 tcgataaacg gcttccctga gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa
     3241 tattacagcc attgcaccgt gactcgtcat gaatcatggc ataccgaact tccttttctt
     3301 gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa aaagattact gttcccatcc
     3361 ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc ccctaatgca gtggctgcaa
     3421 ctgtccaccc tatccatgac atctccctga aatttcaggg gttctgaaac tgctctacct
     3481 cgaaccattc gcataagcca taagccttgc cataggtcat aaatcgctcg ctaaacaagt
     3541 acaacaccgc cgtcgatgac tccacctgcc gtatatcggc atattcaggt gcaacatcca
     3601 tcgcggcggt cgcggcttca atctgcgctt cctagaagta atatggcgct tgcatcagca
     3661 tggcgacttt gtaaggacga ggatacgtct ggcactcaaa acgaacggcg tgtgcaatgg
     3721 cgcgacaaat gtcctgctcc acaacttgca aagacatcgc cgcgtagttt tcactcatgg
     3781 cctgcgttga gtaatagtaa tcatcctgac ttcccgtcat acaagcgata tccgcgtagt
     3841 ctccgttctg cttaatgctt tccagcaaaa ctgacagttc ctcttctgca accgaatagg
     3901 gtggcgagag aaacacggtg cgcgccacca gctgcccagc cgcagaatgc tggcgaatga
     3961 actcagccag gatttcagca ggttctggtt cggttaccgt atcaacgggg tgacgcatcc
     4021 cctgcaacca ttgccgcttc gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat
     4081 cctgatggtt catcttaccc attccttacg caggcagtag attgtgtgct gccagatcgg
     4141 cggcaatatc ttccagcccc agacgctgca atgtttcgcg ggttggacaa ccaagctgtg
     4201 ggtcccagcc catttctttg tagaacatcg tgagcgaagc atgcatatcg tcacgatcca
     4261 ttttgtcagt accttcagta aagaccggga tctgcggatc cttgtcgaaa acccaggaac
     4321 agataagatc gtgttcatta cgcatatctt tggtttgcat cggttttacc gtgtaggcac
     4381 gatgcaacgt aaagatacgc tctgcagcta aatctaattt ttcctgagtc atctcttcgc
     4441 cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc gccccggtaa ttacggcttt
     4501 tcagcggcga aacggtcatt ggccagaccc agttgcacag ggtgacggcg ttatgcaaac
     4561 agacccgcaa cagcgaccac tttgcatatt taatttttgc gtcattgatt ggcgtgtagt
     4621 ttttggtttc atcgtaagca tcttcagaac caaaaagttc tttcgccact tcacgttgca
     4681 gtttcaatgg caagccggaa ccaataaagt tgatatgggt atgcgtcatg cagtcacggt
     4741 tgaacataca gttaacaatg aaaccgacct gcgctgacgc ttcattggca tggtgaaccg
     4801 gatagccaaa cggcgaccag agtttatttt tcgcgtagcc ccagtactct ttacccaaat
     4861 tccagcgttc tgcgatggca tatgaaccat aagccaggtg actcagctca cccacacgat
     4921 gcgccagacg gtagtaaaaa tctttaatga agttaacgtc acccgcttcc agttgatccc
     4981 agcgaatttc tgcatactct tcagctggca gaacacgctt gaacacacct ttgctgtaac
     5041 aataagtaaa gtcgcgatgc aactgcccgt agttacacca taggccgtag tcatcgaaca
     5101 aattcagacc aaccaggtta ccaatcacac gaccatcgtc tttatcttca aaatctttcg
     5161 gcccgttcgg gaagatggtg gtatggacaa agtttgctac acaagtgtta ccacctgtgc
     5221 tgggcacgcc aaactctttc acccgaggaa tattcatttg ggtcatacaa cggatcgggc
     5281 aagagtggca gccgctcatt tttactgtgt atttctctgc cgccggtcct aagtcaaaaa
     5341 cggatttata ggtacgaaag ccgaccgtat tttgattgcc tggcggaatt tcacccgttt
     5401 caatcggacc accttcagcc gcgccccaaa acagcccttt acgtgctgtc cagcgtgact
     5461 tgggatctga atactctgcc cacgattgtg gcgtacttgg cacgacatgg ttgttattcg
     5521 caccaataag ttcagtcatc atgtaatcat tgagacgctt catctcctga cgatcggcaa
     5581 tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag gtttttcgaa cccattattg
     5641 cgccagttcc cgcaccgccg ctgtggttac ggctattcaa catgccagag agaggaataa
     5701 ggttttcccc agcctgacca atagccgcca cacaggtttc ccgactggtc aatcgacaaa
     5761 tttcttccgt cgttgcgcgc gtcccttttc cccataagaa atcggctttt tccaggctaa
     5821 ctttgtcatc tttaatcttc agccataccg gtgatttcgc cttcccttcg ataataatga
     5881 cgtcgtatcc agcgaatttc atttgcgctg caaaaaagcc acccatatgg gcatcgcgac
     5941 taaatttcct ttggtaa
//
LOCUS       DAJTRQ010000182         5939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000182 DAJTRQ010000000
VERSION     DAJTRQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.184"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(160..930)
                     /locus_tag="OO332_003837"
     CDS             complement(160..930)
                     /locus_tag="OO332_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944111.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(975..1409)
                     /locus_tag="OO332_003838"
                     /pseudo
     CDS             complement(975..1409)
                     /locus_tag="OO332_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1421..1642)
                     /locus_tag="OO332_003839"
     CDS             complement(1421..1642)
                     /locus_tag="OO332_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944112.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1643..2326)
                     /locus_tag="OO332_003840"
     CDS             complement(1643..2326)
                     /locus_tag="OO332_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5944113.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2390..2701)
                     /locus_tag="OO332_003841"
     CDS             complement(2390..2701)
                     /locus_tag="OO332_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944114.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2698..3600)
                     /locus_tag="OO332_003842"
     CDS             complement(2698..3600)
                     /locus_tag="OO332_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5944115.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4040..4999
                     /locus_tag="OO332_003843"
     CDS             4040..4999
                     /locus_tag="OO332_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5944116.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            4999..5394
                     /locus_tag="OO332_003844"
     CDS             4999..5394
                     /locus_tag="OO332_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5944117.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5577..>5939
                     /locus_tag="OO332_003845"
     CDS             5577..>5939
                     /locus_tag="OO332_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5944118.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAE"
BASE COUNT         1607 a         1563 c         1447 g         1322 t
ORIGIN      
        1 aagccgaggg gatttttagg attatcaggt gcggaactca tcgaggcgcg ggcatccctt
       61 gcccacagag acgccggata gatttaagca agcccaccat taatcaaaaa tcagtgttgc
      121 aaaaacgggg gtgaccatag attttccccg ccatcgtcat catttccgga tttttgccag
      181 ggcatccgcc atgctgcgga tttcggcccg ggcttcccgg acaacttccc gcagaacacc
      241 acggtcaaac caccggcggg gcggttcatc cggtatacgc agcacatcgc aggccagccc
      301 actggacagg atatgctcaa tacaatcgct gaccagatac atttccaccg ccgaaaaacg
      361 tgcgccccgg ttcaggatgg catcgagcac cacgggcagg ttttcatcgt cttcatcgag
      421 ccagcagggg tgatcgcttt ccttcacgac caggcgaaag cggcgcgtct ggcgggtcag
      481 caacgtctga ccgcttgtgg tcagcgccgc ccggatacgc cgcgcatctt ctgccccccg
      541 gcaactgaca ctgcatactg catcgcgcac cgcatcaaga actgccctga tttcgtcttc
      601 ggtcagccat tcacaggcat acgggaaaga cacctgcgtt ccgtccgccg gaagtagcgg
      661 cagcgactgc cactgactgc cggacagaac aagttccgct ccggttccgg tcagtttctg
      721 aatggcggag gccaggtgcg gcggcagcac gccatcgtgg cggttaacga gtaacgtcgc
      781 cagccggggc gcatccagcc gcacgtaaat gctgatccag cccggatagt tcgggttaca
      841 ttggtcaaca tccgtctctt ccggccagcc ctgcgccagc agcaggttac gggcgtgacg
      901 acaaatcaga gcattcagcg tttcattcat aattcatgaa ctccctgtat cgccgcccgc
      961 aggcggcgat aacatcagtc gttttcgtgt gtgttgtttt tcagcaggct gcgggcctga
     1021 tcaaccatcg cctgtcggcg ggtaacgtat tccgggaggg ggaaaccgtg atcccgggca
     1081 gcccattcac gtttccacag tgcctgttca gcgatctgag tcagggtctc cagcagttca
     1141 gcagggacgg tcacctggcg gttttcgcgc acgttgctga taatctccat cgccgcggcg
     1201 gaagaaatac cggactcaat ccgctagtct tccgggatgc cctccaggtg cgccaggacg
     1261 gtcctgattt catgatcgga cagggaaata tccattccgt cagctagggc ctgaatctct
     1321 tccggggacc agataaccag catcagcggc gtgtctcccg aatactttgc tgccagcgcc
     1381 cggcatagcg tttcgcatgt tccgtacata ctctctcccc tcaatctttg aagcaggaat
     1441 ccagttccag aatttcggta tatgcctcca tcaagaggcg gtttgcgcga aacaacgtct
     1501 gacgcaccag acgttccggc agggtgtcag cattaaagct gtaccgccgc tccatctcat
     1561 tcatggttgt acgcaggtcg cacatggcat tggccacctc cgccacttcg gcgcgaagtt
     1621 tttcgtgtcc ggcatagttc atttacgcag cctccggcat aaaccagtca tcattcgcgg
     1681 ccccctgctg catggcccgc tgtacggcag ccagtcgttg ctgtccggca cggtgatact
     1741 gctcaagcag ctcgataccg atataccggc gtccggactg gagggcggcg acgcaggttg
     1801 aaccgctgcc tgcaaacggg tccagcacta tcgcattcgg gtgtgtgaag ctctcaatca
     1861 gcggttgcag gctggtaacg ggcttttccg tcgggtgatg gcggttgccc gaatatttcc
     1921 agcccaacac gtccggcagc gggttttgtg gcagacgtgg acggcctttt gccaggatgt
     1981 aggcgcattc gtggcgatag cccacatatg cggccttcga tgtgtagttt ttggtgaaga
     2041 ccaggtgacc aacaacgctg aatcccgcat ttttccaggc agacataaag cgatcgacgc
     2101 ggttccagcc atagaagctc accatcagcg cgtctttttt gagtacgcgg tacatttcat
     2161 tacaggccgg ttgcagccat tcatcagttt tatcaccagc aatggtacgc ccctgacggt
     2221 cacgaaaacc gacgagatat ggcggatcgg tgaggatgaa atcaatggcg ttatcaggaa
     2281 tacgggccat aacatcgatg cagttaccga ggacaaaacg ggacataaca gatccttctc
     2341 ttacagaggg cacccctgtg gtgcccgttc cccgctgcct gtgggctgtt catcgcgtcc
     2401 ggtaacgggt caggccgacc gtggaatacc gcagccgtcg tctttttgac agcaaggtta
     2461 tgccgcgaaa gcggcttgaa gggcgttcat cagaaaaggg cgacgggctg gcgtacactg
     2521 tcaccgacgc cagccgggag cggtaaaagc gcccgtcccg tgcccgccgg acgcgggaca
     2581 ctgaccacag gcagcgttta tgccgcgccg cgtcagccgg cgcagtaaaa agaggaacac
     2641 ggcgacatcc tttgtcggaa aagaaacggg cggttgcccg cccgtctccg tcatacttca
     2701 tccgccataa tgcgccacat tgtcgactat ccacgcaggt ccggtgactt ccggctgctc
     2761 atcgtcatct gtcggggaag accattcaag ttccccccac agcacatcga cgagctctcc
     2821 acgctcatac aactgccagc cacagaaatc acagccctgt tcggcatacc agtgctccag
     2881 cgtgcagctg aaacggcggc ttaattctgc aataacgtcg ctttccggct gacaccacgg
     2941 cgtgtcgaaa tccacctgaa tgaagttttc tccctgactg ctgatattca ggtcatatcc
     3001 acaaggccac ttcacgccat accgttccgt ataccagtga tatgcagaag gaacaccgtt
     3061 cagaacacca ccattaaagc cgttcacttc aacatccagg cgggtcggca gaaccgtcag
     3121 caggtcaaac ggcatggttt tttccggcac tacgttgtca cacagacggt tccaccacac
     3181 agatacatcc tcgttacccc atatgtcaca ccagtcacct ctttttgcgg tgaaatgcac
     3241 agtgatggtt tccctggcat catccggtaa tccttcccag cgacgctggc cagtaccgct
     3301 ctgtaaccag agttcatgca gcatcaggca gttttgttca tccagcaaca caccgttctg
     3361 caggtgtgtc agccagcggg tgaacgcgat attttccgtc gatacagcac cacgtccggc
     3421 agccgtcact ccggggcact gttcagagcg gatattttca gtgatctgca gaagtccggc
     3481 acttccggcc agaaacagct gaataccttc atttgtggcg cggcgataaa gcggtgtgac
     3541 tgcaccgctg gccagtcgtt taatctcagc gatctgtgcc ggttcaccag gaaaatacat
     3601 acgattcgag caccagtttg gcatgggaat tctcctctca catgcggcct ccatcaggcc
     3661 gtgccgcacc tgtaaccggg cgaccgcata cgaatgcagg tgcggccccg aagccgcagc
     3721 gcgtggagga ggaatgacga aacgtgaccg ggtgaggagg ccggcgacag cgacacactt
     3781 gcatcggatg cagcccggtt aacgtgccgg cacggcctgg ttaagtagta tccgtctcag
     3841 ttcgtggcgc aaaatgagaa accaataaga actcaaaatg aaccacaaca agaacataaa
     3901 gagactctaa aagagcaaca ccgcttgaca tatactcaaa atgagtcaca ataagaaaca
     3961 ataaaaaact caaaaagatg caccgatata aaggatgctc aatttgagct aaacacaaac
     4021 tcaaaacgag gcaaaaagaa tgctaaaagt atcatgcgat gatggttcaa ctaacgtaaa
     4081 actggcctgg cttgaagatg gcgaggtaag aaccagtctg tcaggaaaca gctttaaaga
     4141 aggctggaac ccgggactgt ttaatgccgg caaagtttac aattacgtcg tggatgaaaa
     4201 aaaatacacc tatgatttgg gaagcactgc tgtcataggt acaacacatg tcagttatca
     4261 atacagcacc accaatctgc ttgccataca tcatgcactg ctgacatccg gccttcagcc
     4321 acaggatgta gaactgacag taacattacc tgtgaccgaa ttctttgata atgacaatca
     4381 accaaatgaa gaaagaatag aaagaaaaaa ggcaaatgtt ctccgtgaga tatctctgaa
     4441 taaaggggaa acatttaaaa ttaaaaaagt taatgtaatg cctgaatctt tgccagctgc
     4501 atttgaatca ctaaaaaaag ataaagtaaa caaacttgaa cgcagcctga ttatcgatct
     4561 tggaggaacc actctggatt gtggtttaat tctcggtgca tttgaaggca tttctgaaat
     4621 ccgtggctat tcagaaatcg ggacatcccg catcactcat accgtcatga atgcgctgac
     4681 gaaagcctcc acaccatgta attattttat tgctgatgag ttaataaaaa acagacacga
     4741 taatgaatat ctccagacat taataaatga tgtagctgaa ataaaaaaca tttctcatgt
     4801 aatagacaga gaagtgaaat ctctggcaga aagcatcagg caggaaattt caacattctc
     4861 aggaatgaac cgaatctatc tcactggcgg aggagccgag cttatttacc ctcacataaa
     4921 gcaatatttc ccgaatctga aagtgaacaa ggtggatgaa cctcagtttg cattggtgaa
     4981 agcaatggtg catgcgtaat ggaaagcagt gatccaaaaa aaagaaagaa agttgtggca
     5041 tacctgcacc cggcacttta tccgcaggac aacctgacac aacaaacaat agactctctc
     5101 ccagtacaaa tgcggggaga tttctacaga caatccctta tttgcggagc agctctttat
     5161 tctgttgacc cccgactgtt aacgctaatc agcggttttt tcagtgagaa aatcactgca
     5221 gaaaatctgg tgaaacttat agaacaaaca acaggataca catccacatc aattgatata
     5281 agtgtgttaa aaaacatcat agaggcatct tcagaaaaca aatcagagag catcacttca
     5341 aaagatgatt tcgaagaaca aaccagacgc aatctttcta tgttaaaaaa ataataaaag
     5401 aagagcaggt tcagtcaatt acctgctctt cttttataca tactaaaaaa tcagattttt
     5461 acgaatattc agattatcat aaaaaatgca acagacagga atgcaataat tttgcattat
     5521 ctcgttgctt gtaatgcaaa tatgcattat tgtcagtgtg tgacaataac agagagatga
     5581 cgatggcgac cattaacgca cgtattgatg atgatataaa taaccaagca gatgaagtgc
     5641 tgaagctgat gaatatcagt caaactcagg ctattgcagc tttttatcag tatattactg
     5701 aacaaaagaa actgccattt gtgatcacct caattgtaaa aacgccgcat gatttattac
     5761 gtgaaagtac agacatgtta gctgaagctc ttgcagtgat ttccaatcta caggtgtgga
     5821 ctgaacagca ggatgggatt ggaaaagcaa agctaatgga gtattaccgc cgcctcgacg
     5881 cactgtactg ttgtgcaaaa gagaaaatag gtcttctttc agataaccgg gatgctgaa
//
LOCUS       DAJTRQ010000183         5936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000183 DAJTRQ010000000
VERSION     DAJTRQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.185"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            159..368
                     /locus_tag="OO332_003846"
     CDS             159..368
                     /locus_tag="OO332_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR5944119.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            361..1083
                     /locus_tag="OO332_003847"
     CDS             361..1083
                     /locus_tag="OO332_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCR5944120.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            1083..1373
                     /locus_tag="OO332_003848"
     CDS             1083..1373
                     /locus_tag="OO332_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5944121.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1370..1732
                     /locus_tag="OO332_003849"
     CDS             1370..1732
                     /locus_tag="OO332_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5944122.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            1729..1917
                     /locus_tag="OO332_003850"
     CDS             1729..1917
                     /locus_tag="OO332_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5944123.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            1914..2537
                     /locus_tag="OO332_003851"
     CDS             1914..2537
                     /locus_tag="OO332_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5944124.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(2806..3024)
                     /locus_tag="OO332_003852"
     CDS             complement(2806..3024)
                     /locus_tag="OO332_003852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944125.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            3249..3572
                     /locus_tag="OO332_003853"
     CDS             3249..3572
                     /locus_tag="OO332_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5944126.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            3556..4032
                     /locus_tag="OO332_003854"
     CDS             3556..4032
                     /locus_tag="OO332_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5944127.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            4029..4496
                     /locus_tag="OO332_003855"
     CDS             4029..4496
                     /locus_tag="OO332_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5944128.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            4484..4636
                     /locus_tag="OO332_003856"
     CDS             4484..4636
                     /locus_tag="OO332_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944129.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            4842..5042
                     /locus_tag="OO332_003857"
     CDS             4842..5042
                     /locus_tag="OO332_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5944130.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            5066..5347
                     /locus_tag="OO332_003858"
     CDS             5066..5347
                     /locus_tag="OO332_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5944131.1"
                     /translation="MWGVELLAIRYAAWIKPEFEIEVYEVFKTVVRLGVGAMSRLNRI
                     DHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEVQMYLPGMV"
     gene            5604..5723
                     /locus_tag="OO332_003859"
                     /pseudo
     CDS             5604..5723
                     /locus_tag="OO332_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
     gene            5789..>5936
                     /locus_tag="OO332_003860"
     CDS             5789..>5936
                     /locus_tag="OO332_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944132.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDE"
BASE COUNT         1720 a         1352 c         1526 g         1338 t
ORIGIN      
        1 agttcgtcag gcaaagagaa aggccaatac gacatacatg aaggcgtatc gaaaagcatg
       61 gaaagaacac cgcgatcgat accaacaaga catggaaaag cttgaatcag aaaacatgga
      121 attaagacga aagctcggtg aagcaaaacg agacatcgat gcttacaagc gactttttaa
      181 tggtgaaagc catgcttagc ccatcccaat cccttcaata ccagaaagaa agcgtcgagc
      241 gagctttaac gtgcgctaat tgcggtcaga agctgcatgt gctggaagtt cacgtgtgtg
      301 agcactgctg cgcagaactg atgagcgatc cgaatagctc aatgtacgag gaagaagacg
      361 atgaatgagt taataaatgg caatgccatc aaaatgacaa gcattgaaat cgctgagttg
      421 gttggtaagc gtcatgacaa tgtgaaacgt accatcgaaa cgctggctaa aaatggtgtt
      481 atccggcttc ctcaaattga ggattgtgga agaatcaatg ggttaggctt aaatcaaagt
      541 ttttgtgtgt atgtgttcga aggcgaacaa ggaaagcgag acagtattgt cgttgtagcc
      601 cagttgtcgc cggaattcac cgctcgcctt gttgaccgct ggcgagagct tgaagaagct
      661 gcggttaata tccccaaaac gctaccggaa gcgttgcgcc ttgctgctga tcttgctgag
      721 cagaaaatgc aactggaaaa ccagctcgca attgccgcac ctaaagttga gtttgccgat
      781 cgcgttggcg aggccagcgg aattttgatt ggaaactttg caaaggttgt tggaattggt
      841 ccaaacaaac tgtttgcgtg gatgcgcgat cacaaaatcc ttattgcttc aggttcccgg
      901 cgcaatgtgc caatgcagga atatatggat cgcggctatt tcacagtgaa agaaacagcg
      961 gtcaacacaa atcacggaat acagatatcg ttcaccacaa aaatcaccgg gcgtggtcaa
     1021 cagtggctga ccagaaagct gctcgataat ggaatgctga aagtaacagg ggaggctgct
     1081 taatggctaa tctacgcaaa gaagcacgcg gcagagaatg ccaggtacgt atttacggcg
     1141 tatgcaatgg taatcctgaa actacagttc tggcacatta ccggatggct ggaatttgcg
     1201 gaacgggaat gaagcctgac gacctgatcg gcgcatgggc ttgtagcgcg tgtcacgatg
     1261 aaatcgaccg acgcacccat aatctcgaca acaaagacgc cagactttac cacctcgaag
     1321 gcgtgatcag gacgcaggcg atactgctga aggaggggaa gattaagtca tgagcgaata
     1381 tcagtttgtg cttccatacc cgccgtcggt gaacacctac tggcgaagac ggggaaacca
     1441 atactacatc agcgataaag gccagaaata ccgaaaagaa gttcagcaaa taatcctcca
     1501 actcaagtta gacattttca ccaaatcacg actccgcatc aaagtcatcg cagacgttcc
     1561 agactcccgc cgccgcgacc tcgataacat cctgaaaggt ttactcgact cccttatcca
     1621 cgccggattt gcggaagacg acgagcaatt cgatgacatt cgcgtaattc gtggtgtgaa
     1681 agtaccaggc ggaaggcttg gaataaaaat caccgaactg gagaacgtat gaacgccaca
     1741 attcaaacga taccagagct tcttatccag acacgaggca atcagaccga agtggcgagg
     1801 atgctttcct gcgcaagagg aacagtgctc aagtacaacc gagacagcaa aggcgagcgt
     1861 cacgtaatag ttaacggcgt cctgatggtc aaacagggca agaggggaag acgatgagac
     1921 tcgaaagcgt agctaaattt cattcgccaa aaagcccgat gatgagcgac tcaccacggg
     1981 ccacggcttc tgactctctt tccggtactg atgtgatggc tgctatgggg atggcgcaat
     2041 cacaagccgg attcggaatg gctgcattct gtggtaagca cgaactcagc cagaacgaca
     2101 aacaaaaggc tatcaactat ctgatgcaat ttgcacacaa ggtatcgggg aaataccgtg
     2161 gcgttgcaaa gcttgaagga aatactaagg caaaggtact gcaagtgctc gcaacattcg
     2221 cttatgcgga ttattgccgt agtgccgcga cgccgggcgc aagatgcaga gattgccacg
     2281 gtacaggccg tgcggttgat attgccaaaa cagagctgtg ggggagagtt gttgagaaag
     2341 agtgtggaag atgcaaaggc gtcggctatt caaggatgcc agcaagcgcc gcatatcgcg
     2401 ctgtaacgat gctaatcccc aaccttaccc aacccacatg gtcacgcact gttaagccgc
     2461 tgtatgacgc tctggtggtg caatgccaca aggaagagtc aatcgcagac aatattttga
     2521 atgcggtcac acgttagcag catgattgcc acggatggca acatattaac ggcatgatat
     2581 tgacttattg aataaagttg ggtaaatttg acccaacgat gggttaattc gctcgttgtg
     2641 gtagtgagat gaaaagaggc ggcgcttact accgattccg cctagttggt cacttcgacg
     2701 tatcgtctgg aactccaacc atcgcaggct gagaggtctg caaaatgcaa tcccgaaaca
     2761 gttcgcaggt aatagttaga gcctgcataa cggtttcggg attttttata tctgcacaac
     2821 aggtaagagc attgagccga taatcgtgaa gagtcggcga gcctggttag ccagtgctct
     2881 ttccgttgtg gtgaatgcac aggctgatgt gtaagggcaa gaatctttcg ctggattcgg
     2941 tgtggccacg tagccagctg taggcagttg cagcaaaccg gagatcagca ccggtcgcca
     3001 caatccaaac tgagccgtag ccactgacta tcctgaattc atcagtgata gttacgctgc
     3061 ggccttctac acatgatctt cgtgaaagtg ggcggcatga ggttgcgcta acaacctaat
     3121 gccgttttgc ccgtgcatat cggtcacgaa caaatctgat tactaaacac agtagcctgg
     3181 atttgttcta tcagtaatcg accttattcc taattaaata gagcaaatcc ccttattggg
     3241 ggtaagacat gaagatgcca gaaaaacatg acctgttagc cgccattctc gcggcaaagg
     3301 aacaaggcat cggggcaatc cttgcgtttg caatggcgta ccttcgcggc agatataatg
     3361 gcggtgcgtt tacaaaaaca gtaatcgacg caacgatgtg cgccatcatc gcctggttca
     3421 ttcgtgacct tctcgacttc gccggactaa gtagcaatct cgcttatata acgagcgtgt
     3481 tcatcggcta catcggtact gactcgattg gttcgcttat caaacgcttc gctgctaaaa
     3541 aagccggagt agaagatggt ggaaatcaat aatcaacgta aggcgttcct cgatatgctg
     3601 gcgtggtcag agggaactga taacggacgt cagaaaacca gaaatcatgg ttatgacgtc
     3661 attgtaggcg gagagctatt tactgattac tccgatcatc ctcgcaaact tgtcacacta
     3721 aacccaaaac taaaatcaac agccgccgga cgttatcagc ttctttcccg ttggtgggat
     3781 gcttaccgta agcagcttgg cctgaaagac ttctctccga aaagccagga cgctgtggca
     3841 ctgcaacaga ttaaagaacg tggcgcttta ccgatgattg atcgcggtga tattcgtcag
     3901 gcaattgacc gttgcagcaa tatctgggct tcacttccgg gcgctggtta tggtcagttc
     3961 gagcataagg ctgacagcct gattgcaaaa ttcaaagaag caggcggaac ggtcagagag
     4021 attgatgtat gagcagagtc accgcgatta tctccgctct ggttatctgc atcatcgtct
     4081 gcctgtcatg ggcggttaat cattaccgtg ataacgccat gacctacaaa gagcagcgag
     4141 ataaaaaagt cagtgagctg aagcaggcga ccgccaccat tactgacatg cagcaacgcc
     4201 agcgtgctgc tgatgcactc gatgctaaat acacgaagga gttagctgat gcgaaagctg
     4261 aaaatgatgc tcttcggcgc aagcttgata atggtggcag ggtgctcgtc aaaggaaaat
     4321 gccctgtgcc atcctcagcc gaaacctcca gcgcctccgg catgggcaat gatgccaccg
     4381 tcgaactctc tccagttgct ggacgaaacg ttctcggtat ccgggacgga attatccgcg
     4441 accaaacagc actgagaacg cttcaggaat acatcaggac gcaatgcctt cgatgatagc
     4501 gataatttta ctcatcatcc ttcacatctg gctctgtaga caggggggtg atcacttctg
     4561 gagtaaatcc agattaaaca tctcattgct gatgcttgat attgagcatc tggcgcgcgg
     4621 taaggggctg cgttgagata agagccagtc atcacaaaca ccaggattta gcctcgcatt
     4681 cgcggggctt ttttatatct gcaacaaacg cgcttcacac gcgcgactta tgaacacaga
     4741 gcctttcagg atgacccttg aggatgccgg tttggtaatc ggtgcctttc tgtgggccgg
     4801 aatcctgtgt gacaaggttc atcactaaaa ggtaatcact gatgaagtac ccaacagtta
     4861 ttgtcaatgg tgtgtccgtt cgtgttgatg aggatggacg ctacaactta aacgatctcc
     4921 atgcagcagc agttgcaaat ggagaggcta cagagcaaca gcgcccaagc cagtttttgc
     4981 gtagcgcgca gataaaacgc ttcataaaag cactttggaa caaattcaac cacttaaaat
     5041 aatcaaaggt ggtgcagaac caggtgtgtg gggtgttgaa cttctggcaa tcagatatgc
     5101 agcatggatt aagccggaat ttgaaatcga agtttatgaa gttttcaaaa cggttgtccg
     5161 tctcggcgtt ggcgcaatgt cccgtctgaa tagaatcgat cacatcatca atactgaaac
     5221 caaagcgata agccagtgcg caagccaaat ggctaagtgg ggcgttggtg ggcgaaaaag
     5281 attgcttcat gttgcacgtg agagagcggc aaatgaagtg caaatgtatt tgcccggaat
     5341 ggtgtgattt cgcaggttaa tccagttttt gcattacggc agtaccgcga aacaacccaa
     5401 gccagtaagt ggggaaataa cactggcagc cactgaaaga tgaacctcca gccttatggc
     5461 aaaaaagatt ctttgtggtg gcggactgat ggaaagacat cggttattgc agaggccatt
     5521 caatgagtgg tctcgataat ggcttatacc ctacacggga taacttaact gatatccctt
     5581 ttaacggata aacggagcca acaatggcag agattattcc catgactgaa gaacagaaat
     5641 tccagttaga gatttacaaa ctggttatga accagaacgc agccgcagaa gaagcatttc
     5701 agttcattgg cactgatgaa gtggcacact gaatttggcc acctgaacag aggtgatatg
     5761 ctcacctcag aacaacacag gtgctccaat gaaaaaaaga aattttagcg cagagtttaa
     5821 acgcgaatcc gctcaactgg ttgttgacca gaaatacacg gtggcagatg ccgccaaagc
     5881 tatggatgtt ggcctttcca caatgacaag atgggtcaaa caactgcgtg atgagc
//
LOCUS       DAJTRQ010000184         5829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000184 DAJTRQ010000000
VERSION     DAJTRQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            48..1516
                     /locus_tag="OO332_003861"
                     /pseudo
     CDS             48..1516
                     /locus_tag="OO332_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1552..1755)
                     /gene="ydfZ"
                     /locus_tag="OO332_003862"
     CDS             complement(1552..1755)
                     /gene="ydfZ"
                     /locus_tag="OO332_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5944133.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1933..2619)
                     /gene="ydfH"
                     /locus_tag="OO332_003863"
     CDS             complement(1933..2619)
                     /gene="ydfH"
                     /locus_tag="OO332_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5944134.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2708..3454)
                     /gene="ydfG"
                     /locus_tag="OO332_003864"
     CDS             complement(2708..3454)
                     /gene="ydfG"
                     /locus_tag="OO332_003864"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5944135.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3591..5636
                     /gene="dcp"
                     /locus_tag="OO332_003865"
     CDS             3591..5636
                     /gene="dcp"
                     /locus_tag="OO332_003865"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5944136.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            5701..5829
                     /locus_tag="OO332_003866"
                     /pseudo
     CDS             5701..5829
                     /locus_tag="OO332_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1468 a         1381 c         1505 g         1475 t
ORIGIN      
        1 atataacaaa acatatagct tgccatattt aaatttaagg aattgttatg ggaaataatt
       61 tgttatcagc aaaagcgaca ctccctgttt atgatcgtaa taacctggcc ccaagaattg
      121 ttcatttagg ctttggtgca tttcaccgtg cgcatcaggg cgtgtatgcc gatattcttg
      181 ctacagaaca tttcagtgac tggggatatt atgaggtcaa cttaatcggc ggcgaacagc
      241 aaattgccga tttacaacag caagataatc tttataccgt tgcggaaatg tcggccgatg
      301 cgtggacggc tcgcgtcgtt ggcgtcgtta aaaaagcctt gcacgtacag atggatggct
      361 tagaaaccgt gttggctgcg atgtgtgaac cgcaaatcgc gattgtctct ctgacaatca
      421 ccgaaaaagg gtatttccac tctccggcga ccggacagtt aatgctcgat cacccgatgg
      481 tcgctgccga cgtgcaaaat ccccaccagc cgaaaacagc aacatgggtg attgttgagg
      541 cgctggctcg ccgtaaagcg gcaggacttc ccgcattccc gcatttaccg tcatgtcatg
      601 tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcct acgcgcaagc
      661 cgttgatgta aaactggcac aatggatcga agataacgtg actttcccat caacaatggt
      721 ggaccgtatt gtgcccgcag tggcagagga tacgctggcg aaaatcgaac aacttaccgg
      781 tgtgcgcgat cctgctggcg ttgcctgtga acctttccgc cagtgggtta tcgaagataa
      841 ctttgtagcc ggacgtccgg aatgggaaaa agcgggagcc gaactggtta gtgatgtgct
      901 gccttatgaa gagatgaagt tgcgcatgct caacggcagt cattcattcc tggcatatct
      961 gggttatctt gctggatatc agcacattaa tgactgtatg gaagatgaac attatcgtca
     1021 tgcggcgtat ggcatgatgt tgcaggaaca agcgccgacg ctgaaagtgc agggcgttga
     1081 tttgcaagat tacgctaacc gattaattgc acgctatagc aacccggcgt tacgtcatcg
     1141 aacctggcag attgcgatgg atggcagcca gaaattgcca cagcggatgt tggattctgt
     1201 tcgctggcat ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg tcgcgggttg
     1261 gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg atagaaatca gtgacccact
     1321 gttacctgtt attcagaagg ctgtacaaag tagtgccgaa gggaaagcgc gcgtccagtc
     1381 attgctggcg attaaggcaa tctttggtga tgatttgcca gacaatagct tgtttactgc
     1441 aaaagtgacg gaagcgtact tgtctttatt agcgcatggc gcgaaagcga ccgtggcgaa
     1501 atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg cattcacaca cttagttcat
     1561 gccgagacga atcaggtcca gaggtgccag tttctcttca caaccttcaa caactacggt
     1621 ttttccgcgg cggatttgct cagcggtcag accttcagta tcaatcgaca ggatcttgcc
     1681 agtgtgaccg gtgccgctaa ccataacacg gctgccagtg gtgattgcgt tacggttacg
     1741 atcgtaggtc gtcataggtg ttttctcctt tctgatttac agtcgaagcc cacgtaacgg
     1801 gcgcaatata aatactcctg tcgagtgaaa tattttttgt ttttgatcaa gctcacatct
     1861 tttttgatgc atgcatgagc cgtaaggcgg ggggaaaagc ctctcatccc ggatgggatg
     1921 agagtaaatg aattactctt cgctaaacca gttgctgttt tcctggcgga tctggcgcac
     1981 ggattcgctg atttcctgca aatgttgcgt cattgcacgt tctaccgcat cgccatcacg
     2041 tttttgcagg gcagagaaaa tatcaagatg ctggcgtaac agcatttctg gtggagaaac
     2101 atggtcgaaa ctcatatagc gcacgcgatc aacggtcgct ttcaggttct caatggtatc
     2161 ccacgccagt tgacagtcgg caatctgcgt caggagttga tggaagttgt catcaagttc
     2221 aaaaaaatca tccagttgct tgcgctcaat ggcaatgcgt tgctggtgaa gattttgttc
     2281 cagttggtag cactggcttt cggtaatcat gctcgccgcc cgacgcgcca ccgcgcactc
     2341 aatggcctga cggataaaac tgccgttgcg cacctgggcc atggaaattt tgttgacgta
     2401 gctgccacgc tgcggacgaa tttgaatcag gccgttttcc gccagtttaa taaaggcttc
     2461 acgaaccggc tggcgtgaca cattgaaacg aacagaaact tctttttccg acaacggtgt
     2521 gcctggtgca atcaggcaat ggacaatgtc gcgacgaaga atacgataaa tctgctgatt
     2581 gacaggctgt gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag
     2641 ttaactgcat gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt
     2701 ataaaaatta ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt
     2761 ccagggtatt gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga
     2821 catcttctgg cgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc
     2881 ctttaaagcg gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga
     2941 cgcgcaccgc cgtaccatgc agatccgtac gcagattcag gctaaactga cgaacaaacg
     3001 ctttcgtcgc accgtaaacg ttaccaccgg catacggcca gctgcctgcc gttgagccaa
     3061 tgttaataat atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat
     3121 atactaggcc tttgttgttg gtatcaatca tcgtttccca gtcttcaacg ctggctttat
     3181 gcgcaggctc catgcccaac gccaggccgg cattatttac caggatatca atattgctcc
     3241 actcggcagg gagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt
     3301 gggcgatata cagattatct cccagttcgt cttttaactc ctgcaaccgc tcctggcgac
     3361 ggccagtggc gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa
     3421 aacctgccgt tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt
     3481 gtcaataaca tagcacgtga tgcttaagag cgggtaatac tggtttgcaa gattgcactc
     3541 cgttatcagt aagcctacgc tatggaacac ctcgcgttca ggagaagaaa atgacaacaa
     3601 tgaatccttt ccttgtgcaa agcacactgc cgtatctggc tcctcatttt gatcaaattg
     3661 ccaatcatca ctatcgcccg gcattcgatg agggaatgca acaaaagcgg gcagaaattg
     3721 ctgccatcgc gcttaacccg caaacgcctg atttcaacaa tactattctg gcactggaac
     3781 aaagcggaga attaattacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata
     3841 ccaatgatga attacagcgt cttgatgaac agttttccgc tgaactggcg gaactggcta
     3901 atgatatcta tctgaacggt gaattattcg cgcgggtaga tgctgtctgg cagcgccgtg
     3961 aatccctggg gcttgatagt gaatccatcc gcctggtgga ggttattcat caacgttttg
     4021 tccttgccgg agccaaactt gcacaagctg ataaagcaaa attaaaagta ctgaatacag
     4081 aagctgcgac cctgaccagc cagtttaacc agcggttact tgcagcaaat aaatccggcg
     4141 gtctggttgt gaacgatatc gcgcagctgg caggaatgag tgagcaagag attgcgctgg
     4201 cggcagaggc ggctcgcgag aaaggtctgg ataacaaatg gctgattccg ctgctgaata
     4261 ccacccaaca accggcgctt gccgaaatgc gcgatcgtgc gacgcgtgaa aaactgttta
     4321 ttgcgggctg gacgcgagcg gaaaaaaatg atgccaatga tacccgcgct atcattcaac
     4381 gtctggtaga gattcgcgca cagcaggcga aactgcttgg ttttcctcat tatgccgcat
     4441 ggaaaatcgc cgatcagatg gcaaaaacgc cagaagcagc actcaacttt atgcgggaaa
     4501 ttgttccagc ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttatcg
     4561 ataagcagca gggcgggttt agcgcgcagc cgtgggactg ggcattttat gcggaacagg
     4621 tacggcggga gaaatttgat cttgatgagg cgcagctcaa gccatatttt gaattaaaca
     4681 cggtgttaaa tgaaggtgta ttctggaccg cgaatcagct cttcggtatt aagtttgtcg
     4741 aacgttttga tattccggtc taccatcctg acgttcgtgt gtgggaaatt tttgatcata
     4801 atggcgtggg actggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg
     4861 gtgcatggat gggcaatttt gttgagcaat caacgcttaa tgaaacgcat ccggtaattt
     4921 ataacgtctg caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg
     4981 atgtaataac cttattccat gaatttggtc atacgctgca cggccttttt gcccgccagc
     5041 gttatgccac gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa
     5101 tcaacgaaca ctgggcaacg catccgcagg tattcgctcg ctacgcccgg cattatcaga
     5161 gcggggcagc aatgcctgac gaactgcaac agaaaatgcg taatgccagc ctgttcaaca
     5221 aagggtatga gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgcc
     5281 tggaagaaaa cgaagcaatg caggatgtcg atgattttga attgcgggcg ctggtggcgg
     5341 aaaatatgga tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt
     5401 ttggtggcgg atatgctgca ggttattacg cttatctgtg gacgcaaatg ttggccgatg
     5461 atggttacca gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg cagcgttttc
     5521 gcgaggcgat cctttccaga ggtaacagcg aagatctgga acgcctgcat cgacaatggc
     5581 gcggtaaggc tcctcagatt atgccgatgc tgcaacatcg tggcttgaac atataaacac
     5641 caccaatggc tggcctggag atatcgccag gccagcctgg ttatagcagt aattgcagca
     5701 tgttacgaca atgctccgat ggaaagcttc tggggaacgc tgaaaaatga gagcctgagc
     5761 cactatcgtt ttaataaccg ggatgaagcc atctcagtaa tacgggaata cattgagatt
     5821 ttctacaat
//
LOCUS       DAJTRQ010000185         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000185 DAJTRQ010000000
VERSION     DAJTRQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..436)
                     /locus_tag="OO332_003867"
     CDS             complement(<1..436)
                     /locus_tag="OO332_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5944137.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKD"
     gene            complement(528..1418)
                     /gene="pstA"
                     /locus_tag="OO332_003868"
     CDS             complement(528..1418)
                     /gene="pstA"
                     /locus_tag="OO332_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5944138.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(1418..2377)
                     /gene="pstC"
                     /locus_tag="OO332_003869"
     CDS             complement(1418..2377)
                     /gene="pstC"
                     /locus_tag="OO332_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5944139.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(2464..3504)
                     /gene="pstS"
                     /locus_tag="OO332_003870"
     CDS             complement(2464..3504)
                     /gene="pstS"
                     /locus_tag="OO332_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5944140.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(3752..4825)
                     /locus_tag="OO332_003871"
     CDS             complement(3752..4825)
                     /locus_tag="OO332_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5944141.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(4836..>5601)
                     /locus_tag="OO332_003872"
     CDS             complement(4836..>5601)
                     /locus_tag="OO332_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5944142.1"
                     /translation="NDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
BASE COUNT         1451 a         1439 c         1299 g         1412 t
ORIGIN      
        1 tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca
       61 tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa ggcgatgttg tcgtagatgg
      121 acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgt agcagggcga
      181 tatcctgaga gttggtcagg atgttgtcgc catcaagcag aatttcacct tccgcacgca
      241 gctccgggta cagttcaaac attttgttga aggtacgcag cagcgtcgat ttaccgcagc
      301 cggacggccc gataaacgcc gtaacctggt ttttggcgat atccaggttg atgtttttca
      361 gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag
      421 tttcaaccat actcattgca atcttttcct catcgtacct gatgcttttc gctaatcagg
      481 cttaccgtag ggccgggtaa ggcgttcacg ccgcatccgg caaagtgtta accgtgttta
      541 ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca ggacgcacag ggtaatgatc
      601 aataccccgg cccaggccag ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag
      661 atcgtcaccg gcaggttggc gatcggctgc atcatgtccg tgctccagaa ctggttggag
      721 agcgcggtaa acagcagcgg cgcggtttca ccggcaatac gggcaatcgc cagcaggata
      781 ccggtcataa tcccggacac cgacgctttc agcgtaatcg cggagatcat cttccacttc
      841 ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg gcaccagttt cagcatgttc
      901 tcggtggtgc ggataacgat cggcacctgc aacagcgcca gcgcaattac gcccgcccag
      961 ccggagaagt gctccatctg tgccaccacg atggtgtaga caaacagacc aaccacaatc
     1021 gacggcgcgg agagcagaat gtcgttaatg aagcgaatca cttctgccag ccaggatttt
     1081 cgaccatatt ccgccagata aatccccgcc ataatgccca gcggcgtacc gaataccgtg
     1141 gcccacaaaa ttaacagccc gctacccgcc agggcgttcg ccagaccacc accttccgta
     1201 ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca taccgtcgat accgcgagtg
     1261 atggtggaca ttaaaatcca gatcagccag aacaggccga aggccatcgt cgccatcgag
     1321 agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct gcattttgcg gcgagattca
     1381 gccagcgccg cagtggtttg catttcaacc atagccatta gcgtgccccc tcattcttag
     1441 ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga
     1501 tcaggcccag ttccatcagt gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt
     1561 tcgccagcgc agaggtgata ctattgcccg gcatgtacag cgaggcgctg tcgagctggt
     1621 aggtgttacc gatgataaag gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca
     1681 gcatgatgcc gccgataaca ccatttttgg tgaacggaag aacgatacgc cagataactt
     1741 cccaggtggt gcagccaata ccgtaggccg actctttcat catcaccggg gtttgttcga
     1801 acacatcacg cattaccgcc gcaatgtacg gaataatcat gatggcgagg atcacgcctg
     1861 ccgcgaggat accgatacca aatgcggggc cagagaacag cgcgccaaca atcgggatat
     1921 tcgacatgat attgccgacc ggctcctgaa agtaaacggc gaacagcggc gcaaagataa
     1981 acaggcccca catgccgtaa acgatacttg gaatggctgc cagcagctca atggcgatac
     2041 ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac
     2101 tcaccgggac ggcgatcagc agcgcgataa acgaagtcac caacgtaccg tagatcggca
     2161 ccagcgcccc gtagatatcg ttcggtgcat cccactcttt ggtccatagg aaagccagac
     2221 caaatttctg aatgctcggc caggaggaga tgatcagaga gacaataatg ccacccaaca
     2281 tcaatagcac aatcagcgcc gccagtttta ccagcacgct gaaaattatg tcgccctttt
     2341 tacccggtgg gttaaaagcg ggcttggttg cagccataag ttactcttca gttaaacgcg
     2401 tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac accgtacccg gcctggagtt
     2461 ttattagtac agcggcttac cgctactgtc tttaatattg gtcttccacg cagcgcgaac
     2521 ctgttcaact acactatccg gcaggctagc gtaatccagg tcgttcgcct gtttagcccc
     2581 ggttttgtac gcccagtcga agaatttcag cacctctgtg ccttgttctg gtttcttctg
     2641 atctttgtgg atcagaatga acgtggtaga ggtaataggc catgcatctt cgcctttctg
     2701 gttggtcaga tcctgagcga aggttttgct ccagtctgca ccttttgctg cattagcgaa
     2761 gttttcttcg gtcggactaa ccggtttacc atcagcggag atcagtttgg tgtacgccag
     2821 gttgttctgc ttcgcgtaag catattcaac ataaccaatt gcacccggca gacgctgaac
     2881 gaacgcggcg ataccgtcgt tacctttacc gcccagaccg atcggccatt ttacggtaga
     2941 gccagtacca acgttgtttt tccactcttc gttcactttc tccaggtagc tggtgaagac
     3001 gaaggaagtc ccggagccat ctgcgcggcg tactacagca atgttttgtg aaggcagttt
     3061 cagacccgga ttcagtttgg cgatggcttc atcatcccac ttcttgattt tgcccaggta
     3121 gatgtcgccg agggttttac catccagcac cagttcgcca gacttcagcc ctggaatgtt
     3181 aaccgccagc accacgccgc caatcacggt cgggaactgg aacagacctt cctgagccag
     3241 tttttcgtca gacagcggcg cgtcagaggc accaaaatca acggtattag cgataatctg
     3301 ttttacgcca ccggaagaac cgataccctg gtagttaact ttattaccgg tttctttctg
     3361 gtaagtgtca gcccatttgg catacaccgg cgcagggaag gttgcacctg cacctgtcag
     3421 gcttgcttct gcaaacacag agaaagcact catcgataag gtcgcggcga caacagttgc
     3481 gacggtggta cgcataactt tcataatgtc tcctgggagg attcataaag cattgtttgt
     3541 tggctacgag aagcaaaata ggacaaacag gtgacagtta tatataagga atatgacatt
     3601 tttatgacag aaaggaatta ccaaataact tactgatttt aaatgtgaag atgaaaatcc
     3661 cacatgccat ctttttgtca tgatgcattt aaaagtgata aagaaatggg tgagatgaca
     3721 acgatgtgat gaacaatcgt tgtcagacct attagcgcac aatctcaaca gttatcgtcg
     3781 ctgttgcagt aaattgcccc ggggctgggc gagctccagt aaggctcgca ggagcagctg
     3841 aaaaagttac actacctgat ttttcaccat acactgttga tttatccatg tctgttggca
     3901 actcaccacc gttcactttg atttccttat tatttttgtc gtaaattttt atccccacgt
     3961 tagagtttga agttacgacc attgaattgt ccgtattggg ttcggtagca taaaaagacg
     4021 tactgatatc atcggtattc agtatcccta ggttttcaca ctcaatagtg actgttttag
     4081 taattttatg tgttgttacc gcacttccaa caacagatga aaaatctgca gctggtattt
     4141 ctttaaaatc aaaacaaact ggctttaaat tatttatttc gcaactttgc ggagctgtaa
     4201 tatcgccccg aatataaaca tcagccatcg gctgtgagta atccaccggg gtgtcacggc
     4261 taattgttcc atacaatgtc actatctttg tttcaggaat aatcaatttt cctgcaaatg
     4321 tcttcttgat ataaaaatat actatcgctt tagccccact ggatatactt aaaggttcaa
     4381 tgcggttgca attgtacttt gtattaccag acgggttgtt agcatgatat tcaaaaggtg
     4441 cattcacata cccaacccca agaatatcta tagcaatccc aacatcaagg ttcggattaa
     4501 gatcataata attaatatta gagtgaaccc cagcataagt taatgcctgg tttatttttg
     4561 acgtataata gatatagggg aattctccta cgttaccagt atcacaatga cagcgtccaa
     4621 gatatgagga tccattagtt acagtggtgt taaatgtcgt tccaacctta ttttcgttcg
     4681 cggtaataag atatcccgag aaatcaagcg aactgtgaaa tacgccatta tcaggggtac
     4741 agataccttt aggagctgca agtgctacac tacttaacga gagcataaac acagaaaagc
     4801 accactgttt gatatacttg ttcataaaaa tccttttaat gacatgtcac accctgttca
     4861 attaatccgc tctcactttc tggtgtcgaa agtgaataat tgaagatgca ttttgttgag
     4921 gcgttttctc cccaggataa cgtaacctgt cctttttctg gtagaccaga aagataaagt
     4981 tcgccacggt caccgacaat tcctgaatct gccaaacttg catcttgtat cgcagcaatg
     5041 gctccaaaag gtaccggttt accatttgca tttaacactc tgaagagaac gcgataaccc
     5101 acatgagtca caaactccgc tttaaccact gcaccacgcg tgggaaccat cgtaacgacg
     5161 gtattatcaa gttctatatt cgacttcgag aaatagttat cgctgattgc tacccgattt
     5221 tcatcataag ggtttagctg tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg
     5281 cgcatattat ctatttctaa cccggcggcc ccaggtgctt taacaagagc aatagtttca
     5341 cctaactcct gacttaacgt taccccttcg ctgtgtgcca gaattccccc tctgatacca
     5401 taattcagcc tctgggaatt ttggctgtag ttatagctgc cgttcaccgt gccataagag
     5461 ccatcatagg tcagggacat attaccgcca acctcatcat catgactgtt ataggactgc
     5521 attaaattcc atgaaaggtt atttctttcc agcgccgtac cgctgagtcc gagttgatgt
     5581 gagctaccgg aattatcgtt t
//
LOCUS       DAJTRQ010000186         5554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000186 DAJTRQ010000000
VERSION     DAJTRQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.188"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..638
                     /locus_tag="OO332_003873"
     CDS             <1..638
                     /locus_tag="OO332_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5944143.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="OO332_003874"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="OO332_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5944144.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="OO332_003875"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="OO332_003875"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5944145.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1356 c         1540 g         1281 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac attggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cagcatttcc ttaa
//
LOCUS       DAJTRQ010000187         5544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000187 DAJTRQ010000000
VERSION     DAJTRQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(296..580)
                     /locus_tag="OO332_003876"
     CDS             complement(296..580)
                     /locus_tag="OO332_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5944146.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="OO332_003877"
                     /pseudo
     CDS             614..1420
                     /locus_tag="OO332_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="OO332_003878"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="OO332_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="OO332_003879"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="OO332_003879"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5944147.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="OO332_003880"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="OO332_003880"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5944148.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="OO332_003881"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="OO332_003881"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5944149.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="OO332_003882"
     CDS             complement(4472..5386)
                     /locus_tag="OO332_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5944150.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1397 a         1332 c         1476 g         1339 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacctagaa ccacggcagc ctgacggctg caaattgaaa accgcccctg ttcgtcctgt
     5521 tgttgccgca cgctccgcag aata
//
LOCUS       DAJTRQ010000188         5481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000188 DAJTRQ010000000
VERSION     DAJTRQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO332_003883"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO332_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5944151.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO332_003884"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO332_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5944152.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5481)
                     /gene="mdtF"
                     /locus_tag="OO332_003885"
     CDS             complement(2659..>5481)
                     /gene="mdtF"
                     /locus_tag="OO332_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR5944153.1"
                     /translation="TSPDIAQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAA
                     FISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLV
                     PSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTRLQTPEEFGKILLKVQQDG
                     SQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSA
                     YFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVP
                     VVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEAT
                     HKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFSITLISSMRLSVFVAMSLT
                     PALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLIC
                     AGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQ
                     SVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFP
                     FNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLED
                     TPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRM
                     LPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGD
                     AMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSI
                     PFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQK
                     EGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFA
                     ATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1448 a         1273 c         1287 g         1473 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg t
//
LOCUS       DAJTRQ010000189         5449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000189 DAJTRQ010000000
VERSION     DAJTRQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(127..1476)
                     /gene="lysC"
                     /locus_tag="OO332_003886"
     CDS             complement(127..1476)
                     /gene="lysC"
                     /locus_tag="OO332_003886"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5944154.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2001..3650
                     /gene="pgi"
                     /locus_tag="OO332_003887"
     CDS             2001..3650
                     /gene="pgi"
                     /locus_tag="OO332_003887"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5944155.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4112..4354
                     /locus_tag="OO332_003888"
     CDS             4112..4354
                     /locus_tag="OO332_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944156.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4468..5106
                     /locus_tag="OO332_003889"
     CDS             4468..5106
                     /locus_tag="OO332_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5944157.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5103..>5449
                     /locus_tag="OO332_003890"
     CDS             5103..>5449
                     /locus_tag="OO332_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5944158.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKI"
BASE COUNT         1388 a         1413 c         1393 g         1255 t
ORIGIN      
        1 aaaaaattaa aggcaattat tctccgataa agacgttggt taacagagta caggctcgtt
       61 tattgatgag catagtgaca agaaaatcaa tacggcccga aatatagctt ccaggccata
      121 cagtatttac tcaaacaaat tactatgcag tttttgcacc acctgctcgg catcttcgcc
      181 gggcaccagg aagcacaggt tatggctgga tgcgccgtaa caaatcatgc gaatgttgaa
      241 cggttccagt acgccgaata cctctttgcc aacgccgcag gcttttgaca ggtcattgcc
      301 aatcaacgcg accagcgcca gaccttcttc cacctccacc cggcacagtg cggaaagctc
      361 catcagcaga gattgcgtca gcaacgtatc gccagtggag gttgaaccgg tggtatcaag
      421 ggttaatgcc acgctcactt ctgacgtggt gattaagtct accgaaatat tatgccgcgc
      481 gaggatgccg aaaacttccg cgaggaagcc gcgagaatgc agcatattca ggctgtgcaa
      541 agtgagcaga gtctgattgc gacgaagcgc cagcgcgcgg aacagcggcg gattttcagt
      601 tttattgcac accagcgtac cacctgcgcg tgggtctttg ctggagccga caaagaccgg
      661 gatatcgctg cgtactgcgg gtagcaacgt tgccggatgc agtacttttg caccaaaagt
      721 tgccatctct gccgcttctg caaacgcgat ttcatcaatg cgttttgctg cggaaactac
      781 gcgtggatcg gtggtgtaga tgcccgggac gtcggtccag atatcaacac gagatgcgtg
      841 taaagcctcc gccagcaagg ctgccgtata atcgctgcct ccacggccaa gcgtcgttgt
      901 acgaccttta ttttcgctac cgataaatcc ctgggtgatc actaagcctt cattgaggcg
      961 tgggagcagc tgcagcgcgg ccagttccgc cagcgcggct acatctggct ctgcacgacc
     1021 aaatcggtcg ttggtacgca tcactttacg tacatcaaac cactgtgcct gaacatcgcg
     1081 ttcgcgcagg atttcgacaa acagcagggt cgacatcagc tcgccgtggc tgaccagctc
     1141 atctgtcagc gccggagacg ttgccagcgc cgccgcttct gccagaacag taatgttctc
     1201 cagcagacgt tcaatctctt cacggataac gttcgggtaa cgcagacgtt ccagaatggc
     1261 aaactggatg ttgcgaatag cgtcgagttt ttcgaatcgc tcgccaggtt ccagcccttc
     1321 cgctaaagcg accagcagat tagtgatacc agcagaagcc gagaggacta ctaaacgcac
     1381 gttggcatca gaaagcacaa tatcagcgct gcggttcatg gcgtcaaaat cagctacgct
     1441 ggtaccgcca aatttggaga caacaatttc agacataact acctcatgtc aggggatcca
     1501 ttttcagcct tggcacaagg gaagagcgga agacgggtgg gcgcagagcg atacttcgct
     1561 actattttca cccagaagtg ctccaccact tgcgaaacgc ccgactgcga acgcttctgg
     1621 tgacaaccca ggggattcag cccctgtagc cgatgatgaa cgtggccagc cgttcaatca
     1681 cctcggcgat gcaccccctc aggtgttatc acaggactgg ctcctccaac accgttactt
     1741 gggcaacgcg cctcttctgg cctgcgctag cgcaggtagt acatttataa ataaagggtg
     1801 agcggggcgg ttgtcaacga cggggtcatg cggatttttc atccactcct ggcggtcagt
     1861 agttcagcta ataaatgctt cactgcgcta agggtttaca ctcaacatta cactaacggc
     1921 actaaaacca tcacattttt ctgtgactgg cgctacaatc ttccaaagtc acaattctca
     1981 aaatcagaag agtattgcta atgaaaaaca tcaatccaac gcagaccgct gcctggcagg
     2041 cactacagaa acacttcgat gaaatgaaag acgttacgat cgccgatctt tttgctaaag
     2101 acggcgatcg tttttctaag ttctccgcaa ccttcgacga tcagatgctg gtggattact
     2161 ccaaaaaccg catcactgaa gagacgctgg cgaaattaca ggatctggcg aaagagtgcg
     2221 atctggcggg cgcgattaag tcgatgttct ctggcgagaa gatcaaccgc actgaaaacc
     2281 gcgccgtgct gcacgtagcg ctgcgtaatc gtagcaatac cccgattctg gttgatggca
     2341 aagatgttat gccagaagtc aacgcggtgc tggagaagat gaaaaccttc tcggaagcga
     2401 ttatctccgg tgagtggaag ggttataccg gcaaagcaat cactgacgta gtgaacatcg
     2461 ggatcggcgg ttctgacctc ggcccgtaca tggtgaccga agcactgcgt ccgtacaaaa
     2521 accacctgaa catgcacttt gtttctaacg tcgatgggac tcacatcgcg gaagtgctga
     2581 aaaaagtaaa cccggaaacc acgctgttct tggtagcatc taaaaccttc accactcagg
     2641 aaaccatgac caacgcccat agcgcgcgtg actggttcct gaaagcggca ggtgatgaaa
     2701 aacacgttgc gaaacatttt gcggcgctct caaccaatgc caaagccgtt ggcgagtttg
     2761 gtattgatac tgccaacatg ttcgagttct gggactgggt cggcggccgt tactctttgt
     2821 ggtcagcgat tggcctgtcg attgttctct ccatcggctt tgataacttc gttgaactgc
     2881 tttctggcgc acacgcaatg gacaagcatt tctccaccac gcctgccgag aaaaacctgc
     2941 ctgtcctgct ggcgctgatt ggcatctggt acaacaattt ctttggtgcg gaaactgaag
     3001 cgattctgcc gtatgaccag tatatgcacc gtttcgcggc gtacttccag cagggcaata
     3061 tggagtccaa cggtaagtat gttgaccgta acggtaacgt tgtggattac cagactggcc
     3121 cgattatctg gggtgaacca ggcactaacg gtcagcacgc gttctaccag ctgatccacc
     3181 agggaaccaa aatggtaccg tgcgatttca tcgccccggc tatcacccat aacccgctct
     3241 ctgatcacca ccagaaactg ctgtctaact tcttcgccca gaccgaagcg ctggcgtttg
     3301 gtaaatcccg cgaagtggtt gagcaggaat atcgcgatca gggtaaagat ccggcaacgc
     3361 ttgactacgt ggtgccgttc aaagtgttcg aaggtaaccg cccgaccaac tccatcctgc
     3421 tgcgcgaaat cactccgttc agcctgggtg cgttgattgc gctgtatgag cacaaaatct
     3481 ttactcaggg cgtgatcctg aacatcttca ccttcgacca gtggggcgtg gagctgggta
     3541 aacaactggc aaaccgtatt ctgccagagc tgaaagatga taaagaaatc agcagccacg
     3601 atagctcgac caatggtctg attaaccgct ataaagcgtg gcgcggttaa tcatcatgcg
     3661 tgcctgatgc cgatatgtag gccggataag gcgtttacgc cgcatccggc aaccggagcc
     3721 tgatgccaat atcgacatta atgcatagta tgaggagtta aataacctta aaaacaatgc
     3781 cgacaataag tcggcattgt tttatcagat aaatcccctt gtctgtgatt taacggaaat
     3841 cataccgtga ggttaatcct aaaatagatt tttaatcgtt gtttatttcg gagaatacgc
     3901 agattaatta cttttgtttt tattttaagt ttatgatttt tattgttatt taaatataag
     3961 ttgaaactta tatttgatat tcattccaat tatcctaaaa ctccatcgct aattccccgc
     4021 gccgtaattc gcatgcttta gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa
     4081 atctccattc attcaatgaa gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc
     4141 catatctgcg cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc
     4201 agagtcggca gcaacgtcgg tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt
     4261 aagctcggcg gtgggtgtcg cgctcgcggt aaccggtggc ggtgatggtt ctaataccgg
     4321 gaccaccaca accacgacca ccagtaccca gtaataaagt atgtatcccc aaaataattt
     4381 gagtcattgc atctgtggct tgaagtatga agggatttac cataaccaca ctccggtgtg
     4441 gttattctgc ccctctggag aagagttgtg aagcgacctg cactcattct tatctgcctg
     4501 ctattacagg cctgttcagc cacgactaaa gagctgggca attcactgtg ggacagtctg
     4561 ttcggcacgc caggcgtaca gctgacggac gatgatattc aaaatatgcc ctacgccagc
     4621 cagtacatgc agcttaatgg cgggccgcaa ttgtttgtgg tgctggcctt cgctgaagac
     4681 ggacaacaaa aatgggtcac tcaggatcaa gccactcttg tcacacaaca tggtcgtctg
     4741 gtgaagactt tgcttggcgg cgacaacctg attgaagtga ataaccttgc cgccgacccg
     4801 ctgattaaac ccgcacaaat tgttgatggt gcaacatgga cgcgcacgat gggctggacc
     4861 gagtaccagc aggtacgcta cgccaccgca cgctcagtct tcaaatggga tggcaccgat
     4921 accgtcaagg tcggcagcga tgaaaccctg gttcgcgtgc tggacgaaga agtctccacc
     4981 gaccaggcgc gctggcataa ccgctactgg atcgacagcg aagggcaaat tcgccagtcg
     5041 gaacagtatc tcggcgcgga ttatttcccg gtgaaaacca ctctcatcaa ggcggcaaaa
     5101 caatgattaa acaaactatt gtcgcgttgc ttttgagtgt gggagcgtca tcggtcttcg
     5161 cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga ggccaaaacg ctaacgggcg
     5221 cagagcattt aatcgatctg gtagggcaac ctcgactggc aaacagctgg tggcctggtg
     5281 cggtgattag cgaagagctg gcaacggcgg cagcattgcg tcagcagcag gcgttgctga
     5341 cacggctggc agagcagggc gcagattcca gcaccgacga tgccgccgcc attaacgcct
     5401 tacgccagca aatccaggca ttgaaagtga caggcaggca aaaaatcaa
//
LOCUS       DAJTRQ010000190         5448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000190 DAJTRQ010000000
VERSION     DAJTRQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..312
                     /gene="mddA"
                     /locus_tag="OO332_003891"
     CDS             <1..312
                     /gene="mddA"
                     /locus_tag="OO332_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5944159.1"
                     /translation="DWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGK
                     HVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQLQLDERTEPDAI
                     G"
     gene            309..758
                     /locus_tag="OO332_003892"
     CDS             309..758
                     /locus_tag="OO332_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5944160.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(759..991)
                     /locus_tag="OO332_003893"
                     /pseudo
     CDS             complement(759..991)
                     /locus_tag="OO332_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1077..1250)
                     /gene="ortT"
                     /locus_tag="OO332_003894"
     CDS             complement(1077..1250)
                     /gene="ortT"
                     /locus_tag="OO332_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5944161.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1445..1540
                     /gene="yncL"
                     /locus_tag="OO332_003895"
     CDS             1445..1540
                     /gene="yncL"
                     /locus_tag="OO332_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5944162.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1942..2751)
                     /locus_tag="OO332_003896"
     CDS             complement(1942..2751)
                     /locus_tag="OO332_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5944163.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2961..4386)
                     /gene="patD"
                     /locus_tag="OO332_003897"
                     /pseudo
     CDS             complement(2961..4386)
                     /gene="patD"
                     /locus_tag="OO332_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4408..5114)
                     /locus_tag="OO332_003898"
                     /pseudo
     CDS             complement(4408..5114)
                     /locus_tag="OO332_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5104..>5448)
                     /locus_tag="OO332_003899"
     CDS             complement(5104..>5448)
                     /locus_tag="OO332_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5944164.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1435 a         1386 c         1197 g         1430 t
ORIGIN      
        1 gactggcgta gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc
       61 gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg
      121 gattgcggga agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ttcgctgcat
      181 ctgcaccagt cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtagg cactaaattt
      241 ggtcgttggc tggatctgac atttatgcag ttgcaactcg acgagcgcac tgaaccggac
      301 gcgattggat gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg
      361 tagtccagaa cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca
      421 tgttgctgaa ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag
      481 gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacctggct
      541 tgctgggttc gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga
      601 caactattgc ggtactggtc gccagccagc tgattggcgg tctggtgctg gatatcttcc
      661 gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat atgtggcgca attttacttg
      721 tggttggcgc ctggttggtg gccagacgct cattttaatc agaggatggt gccgcctttg
      781 gttaactgtt cgtggccgct tccatatctt ctttatgctt gcgaccgtga tgagcaatag
      841 cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg acttaactcc gcgtcatcgc
      901 tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc gcggcatcca
      961 cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttagccactt gtgaaattta
     1021 tcgattaatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag taagatttaa
     1081 aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgtggcccc caccagaaag
     1141 gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat aagaaatgcg
     1201 attgctgcca taaccgcata aaaaacaagc atatgttgat agagagacat gtacgggcgt
     1261 ccagccatat gaataaaacg cgcgcaaata tgcgccattt tttgacgtgc atcaagtttt
     1321 ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc cttatccatc ttcagaatga
     1381 gtattattgc ggattttctt aacccgtact atacacatag gggcaatgat aaaaggtggc
     1441 aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg ctgttggttt
     1501 gtttactctt atctctatga gatttggctg gtttatttga tgtagtgatt ttgcacgcag
     1561 cacgtactgt cagagaaatc aactctctga cagtacgcaa gatcttctca tactctccaa
     1621 gtttgcaaca ttatccttaa tatttacttc tttttgctgg catctacatt gaaatgagta
     1681 gaaaacatcc ccctactgtc gtctatacaa acagcttact gattcaaacg gaatcttgtt
     1741 ttcacatttt tctatcacga atactttata cgcgcgggag tctataaatg ctttatactt
     1801 tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat atgcatggca ggatgtttgc
     1861 aacctccttg atgacgctgc cccattgaaa ttgcaaaccc cgccctggtt atcttgccca
     1921 tttatcatgc aaaacagata attataggcc tctgaatacc tcagaaagat tgaatttttt
     1981 atagtccctc tcttgaatat ttccatttga tatcaatcga ttcatctctt caacaaaata
     2041 atttatatca tcacacttgt tattatccgg aagatctgag tagttataat tccaccattt
     2101 tattctcaac agttcatcaa taacatttga atcaaaccta aatctgatta tttttgcagg
     2161 aactcctgca actatggcat atgggggcac atctttggta acaacggaat tggcagcaat
     2221 aacagcacca tcacctattg cgatgccacc tttcaataca acatcatttc ctatccagac
     2281 atcatttcct ataatcggat tttcaggttt ggtaatgaat ggtttcaatg tccagctacg
     2341 ttcaaattca ctcatagcaa acttatcaaa ttcaccgtta taagtcatca tgtgagtagt
     2401 aaaacgattc aaaggatgat ttccacccat tatttttaca tttgatgcaa tactacaaaa
     2461 tcgacctata cgtatattcc ctgaaaaatg gcaggtcgga aatgaaaaag cgccgacact
     2521 ggagaaattt ttcacaggca ttgttgaata ttcttcagca ataacattat cttcaattgt
     2581 tatcttgtca ccttttgaga aaagaaataa attcctttct ttatacgact tcttcccctt
     2641 atgatttaaa aagattttat tatccataca aaactcaaag tgttttttcg tccatctgaa
     2701 ggtaagggtt ttatttttat cttccgtgaa tttgtacaat tgaactttca ttgttaatac
     2761 ttctacttat aaattattag tttaataacg atactctaaa gccttatgct aatcaactgt
     2821 gacaggatga aaaatcgcca tcaaacattt agcctaattt ttacgccagt tattaatgta
     2881 atcattctaa tacttaaccg ccattaaact cagaacctaa aacgatagtg gccgtcctat
     2941 aacagccact aacatctaac ttaatgttta accatgacgt ggcggacgac ggtgtaatcc
     3001 tccagcccat aaagtgacat atccttgccg taaccagaaa gtttctgccc accgtgcggc
     3061 atttcactta ccagcatgaa atgggtattg acccaggtac aaccatattg cagccgtgcg
     3121 ctgacgcgat gcgccctgcc cacatctttc gtccataccg aagatgcaag tccgtactgg
     3181 ctgtcattcg cccagttcac cacctgttct tcgttgtcga agggcgtaac actcactact
     3241 ggaccaaata cctctttttg caggatggca tcgtcctgta atgcgccagc cagcagcgtc
     3301 ggcgcatagt aatagccatt acccttgcgc ttttcaccgc cagtgatcac tttgatgtgc
     3361 cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt cgagatgcgc cagcgagctt
     3421 aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag attttaacgt tgccaccgca
     3481 ggcacccagt ttttccacca gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc
     3541 cgcagtacaa tcctgtccag cattgtaata gccaaatgta cgtacacctt cgaccactgc
     3601 ttcaatatcc gcatcatcaa aaacaatcac tggcgctttg ccaccaagtt ccatatgagt
     3661 acgcttaatg gacgacgcgg tatggctgat gatgtgctcg ccggtggcga tagagcccgt
     3721 cagcgacacc atccgcactt tgggatgacc ggtcagcgga tcacccaccg ttttgcctct
     3781 gccaaacagt atgttaatca cgcctgccgg gaagatatct ttcgccagct ctgccaactt
     3841 caacgcggtc agcggggtaa tttctgatgg tttaagcact acgcagttcc ctgccgccag
     3901 cgccggagca agtttccacg cggccatcat cagcggataa ttccacggtg cgatagaagc
     3961 cacgcccccc aacggatcgc gacggatcat cgaagtatga ccttcaaaat attcacctgc
     4021 cgccagacca ttcagacagc gcgccgcacc cgcgaaaaag cgaaaaacat cgacaatcgc
     4081 cgggatttca tcattgaacg cactatgcag cggtttgcca caattacggg attccagttc
     4141 ggcaaaaacc tgaccatttt cttcgataac atccgccagt ttcagcaggc attccgcacg
     4201 cgctttcggt gtggtttgcc cccattcagc aaatgctgca tctgccgcac acacagcagc
     4261 atcgacctgc tctgcggatg cctcggcaat ctccagtaaa acgtcccccg ttgccggatt
     4321 atagacaggc tgtttttccc cttcgccgct aaccagttct ccgttaatca gtaacttatg
     4381 ttgcatagat tattcctgta tcagtggtta ttgaccattg tcgccttcgc gggttagcca
     4441 ccaggccccc aggatcggca aggttgttac caacataacc agcagtgcca ccacgttggt
     4501 taccggtaca tcacgcggtc gcccaagctg attgagcaac cacaacggta acgttcgttc
     4561 atgacctgcc gtaaaggtcg taacgatgat ttcatcgaac gacaaggcaa acgccagcat
     4621 tcctcctgcc agtaacgccg aactgagatt cggcaacact acgtagcgga aggtttgcca
     4681 gccattggcc ccaagatcca ttgacgcctc aaccagactc cacgaggtgc ggcgaaaacg
     4741 ggcgatgaca ttgttaaaca ccaccactac gcaaaaagtc gcatgaccga ccacgatggt
     4801 gaaaaatccc ggctccagat tgatggtttt aaaggcggtt aatagcgcca gaccagtgac
     4861 aatgcccggc agcgcaatgg gcagcagcaa taacagcgaa atggcgtttt tgccgaaaaa
     4921 gtctcgtctc cacagcgcgg cagctgccag cgtccctaac accagcgcag cgtcaggccc
     4981 tgcggtggaa aactaaacgc cgcatcttca gtgttaaacg cataggcggc gattatcagg
     5041 atgggaaaat gtaggaaaac aacgccgccc caggccgcca gtttgaggaa aaacggtgcg
     5101 cgttcagagt gcatcgaacg ctcccagacg tttcacgaac gccaggtaca gtgcgatgag
     5161 aataatcggc accagggtga atgccgccgc catcggcata ttgccaatcg ccccctgctg
     5221 ggaataaacc atattgccga taaaatatcc tggaggacca accagctgcg ggacgataaa
     5281 atcgcccagt gtgagtgaga aggtaaagat agagccagcg gcaatacccg ggattgccag
     5341 cggcagcacc acatagcgaa aggtttgtcg tggacgtgcg ccgagatcag ccgacgcctg
     5401 caacaatgac ggcggcaaac gctcaagcgc cgcctgaacg ggcaggat
//
LOCUS       DAJTRQ010000191         5426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000191 DAJTRQ010000000
VERSION     DAJTRQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..88)
                     /locus_tag="OO332_003900"
     CDS             complement(<1..88)
                     /locus_tag="OO332_003900"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5944165.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(250..3558)
                     /gene="icsA"
                     /locus_tag="OO332_003901"
                     /gene_synonym="virG"
     CDS             complement(250..3558)
                     /gene="icsA"
                     /locus_tag="OO332_003901"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5944166.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4087..5289
                     /gene="virA"
                     /locus_tag="OO332_003902"
     CDS             4087..5289
                     /gene="virA"
                     /locus_tag="OO332_003902"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5944167.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKQLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1682 a         1224 c          900 g         1620 t
ORIGIN      
        1 gtgcattagt ttctgcaata ccagtaatct gaggcgcata accacgaata tttggcggta
       61 gcattcgttc atcgctttct aatgcaattc ctgtgtaacg ccaagcaaca gcgcattctg
      121 gcgcatgatc tggtaaacac gtttggcatt gatcgcaggc ataccatcat gtgcacaaaa
      181 acccaccgag cttttacacg gtgggtccca gagaaatgca ggacatcaac acgccctgca
      241 tttttattat cagaaggtat atttcacacc caaaatacct tgggtatctc tgtaactgtt
      301 attacccaat tgctgagata cgtttcccca gatgcttagg ttatctgcaa gacgcccccc
      361 cacaccagtt ttgagttcaa tcacattacg gttgcctatc tggtgatttg acatgccatt
      421 cataattaca ccatattgct ttgagctcca tttccaattc acttccaaaa atggttcgaa
      481 cttacgaaga gtctccttat cgatacaact ctgactaatt aaggatgtac gcaatcccaa
      541 ttttgcttga atattattga ccccagagga tgtgaccata gtgccattgt gttcaatata
      601 gtcgtttgct tttaccccat gcctagttat ctggacttgt ggattgagat acaatgcgtt
      661 gtcaatatta ttatgagcag tccagcgtat ggttgggtag atatagccag cttccaaagc
      721 tcctgttatt cctttgctgg aatatttctc accagttaac ccatctcctt tgactgtatt
      781 attaaaccaa ctgtactgca accaagcatc catatataga cctgttcgtt ctttttcatc
      841 ctgaaaccaa gaactgtaca aaccggcagt ataaccgctc acagttcctc gtgaactata
      901 actagtatac ttattatgag ttttgctatc ctgatatcca taagctccca taatacccac
      961 atgcaagcca tcatgatcag tgaagtttgt cttcaaaata tctccaccaa tctgataaac
     1021 catcctgtta gctgtggttt ttaattgtct atcagctaaa ttatgctcat tatgtccacc
     1081 aacagtacga atccacatgg tggtttcgct gagctgacct gtagtaggat caacaatagt
     1141 gctaccgtgt cggtcataga gagaaggact aaacatcgtg ttcattgcta tcaggttagc
     1201 aatataactg ccagcttcag ggcgccatgt gtgaatacta ccttcaccca aattaagtga
     1261 agatacaagc tgtgaactag ctcggcgact actcatttga gtagactctt gattgctcat
     1321 ctgttttgat tcttgattat ccatctgact agttagatac cacttattgg tattcagtcc
     1381 agatacagtg ccctgtttca ggcgatagtc ataactacca gcaacaatac ggcctttctg
     1441 aataaaagca ttcttatcag aagaatctgt cgaaataatt tgaacacctt caagagtttt
     1501 atctccagag cctccctcat ttgtcactgc aacataggta ataccagacg catggcctgc
     1561 aatttccagc cggtcagttt tactgttgtc attgccgaga accgcgccca aagataatat
     1621 tccgcccttt ccatgccaat tacctttctg aacataggtc tgaccgacat ttgacgaact
     1681 attattgaga ataacatgac cgtttttctc cagagtcatg ttacctgtga caatgacggg
     1741 gtcaggcaaa aaaggggcat taatattatt gattagcaca gtcccactat ttaataaagt
     1801 gttaacagtt tggttcatgc ctgaaaaatt caaggtagca cctttattaa caataacacc
     1861 agcggtacgt gtcatagctt caactgtccc catttttaga atgcctgcat tgatgttggt
     1921 gaaaccagag taggtatttt tttccgccaa aatgagagtt ccctcccctg attttgttag
     1981 ggattttccg tcccagctat ttatattatg atggctgaga ttatctgcca gaatagtact
     2041 aacagtaagt tcagcgtttt ctttcagatt gaactctcca tgactgtcgc catctgtatc
     2101 attccagctc aaaccgtaga tcaatttatt ctggtcactg atatagcttt ttgtatagtc
     2161 aatagcacca gtagtgagta acggattgat gatagatata ttagaaacac tggaacctgt
     2221 tatcccttta tctgcactta gcagtttata gtccttctgc gcccagttat ttataatagc
     2281 ttccactttt acattgttgc caaatgacat atttccagaa gcttccagac tactgattcc
     2341 agctaaattt aaagaactgt tctcaccaaa agaaacgtca cctgaaacag tgaatttgtt
     2401 acctgatagt gaaacagttg tacaatcacc agcacataaa tcaccttcaa tagttggtgt
     2461 ggtcccagtg taatcattgt tgataatttt cagaatggat gaattgttaa cctgtacatc
     2521 accagtaatg acagaacctt catggagtat taatgaattg tttccaccag tgatgtggat
     2581 agcattacct gctacatttt gtgttccatc atcttcttta cctgaaatta tatatccgct
     2641 attagcaatg gtcatattgc taccatttat agcatccccc caatgtccac cattaccgcc
     2701 taaaatagct ccattgttga ttacagacag gttatctcct gtgatagcat taccaccgta
     2761 gccatcataa ccataaccgt aagcagcacc tccgttgcct ccagcgaacg tgcccttatt
     2821 gattacagag aggctgcttc ctgtgatggc atcacctcca ttaccgccat caccctcacc
     2881 ataattgtta cctccattac caccggaaat agtccctttg ttgattatgg gtagattgac
     2941 tcctgtgacg gcatcacctc cattcccccc atcactatca ctataactat cgccaccatg
     3001 tccacctgaa ataatttctc catttattat aaacagattg tctcctgtaa cagcctcacc
     3061 accatcaccg ttatggtcag caccgctacc gccgctacca ccaagaatca tgccttgatt
     3121 gattatagac aagtcagatc cggtaataga gtcaccacca ttaccgccac aactattacc
     3181 atcattatta tcaccagcac caccatgacc tgcgctaatc ataatatcag agttaatccg
     3241 tagcttagtt ctagatgcat gagaggggtt ggaagaagtt atataccacg catccattcc
     3301 atcttcacca gctcccaagg gagaaagtcc atcaacaggt gttaataatt tttcataatt
     3361 gtcctctgaa aaatgaagtt cttgagtacc cgaaagagga gtagcaaaag ctattggccc
     3421 cccgagcaac agggatgcac caacaacaaa aggagaaaaa gacaatttgc atgttttttc
     3481 ctttaccgta ggtaattctc cggccccccc ctgtgaacat tgggtcatat tacaaaaaaa
     3541 tttgtgaatt tgattcatgc actatattat cagtaagtgg ttgataaacc cctgaaaaga
     3601 ttccgtttta tccggaataa agggacgata tatgcaaaaa catattaaac aaagcctcaa
     3661 ccaaaacatg agagctgtgt tatatatcaa tgcaacggga gaaatttacc tgtgtcttgc
     3721 tttgaaaaaa agaggggaaa agctatatgt tcgataataa attaataatt ttactatgat
     3781 agtctgcttc agaccgcaac ttttcagtcc tttctgtacg cttgcccaaa gtttaaccac
     3841 tttgtttttc tatacaccca atggttctat ctgccccttg atgttaatcc ccaaagccaa
     3901 taacatgaat gtattcatat cacaactttc ctaccagact ctccaatgca ataactgcat
     3961 aattgttttt agtggaaatg tatcgttttc ttaaagagaa gaataacatt ccattttatt
     4021 atgttcccaa tttaattata ttaatatata ttggcattaa taggaaaata catcaggaga
     4081 aatcaaatgc agacatcaaa cataactaac catgaaagaa atgactcatc atggatgtca
     4141 actgtcaaat caactacaga agtaagttgg aataaattga gtttttgtga cgttttactg
     4201 aaaataataa ctttcggcat atattctcca cacgaaactc tagccgaaaa atatagcgaa
     4261 aaacaactaa tggatagttt ctcaccatca ctatcacaag ataaaatgga cggagagttt
     4321 gcccacgcaa acatagatgg catttcaata cggctatgcc ttaacaaagg catatgttcg
     4381 gttttttatc ttgatggaga taaaatacaa tccactcaac tttcatcaaa agaatataat
     4441 aacttgttaa gttcactccc ccccaaacaa ttcaacttag gaaaagtaca tactattaca
     4501 gctccggtat cagggaattt taaaactcac aagcctgcac cagaagttat tgaaacagcc
     4561 attaattgct gtacatcgat aataccaaac gatgattact tccacgttaa agacactgat
     4621 tttaatagtg tttggcatga tatttacaga gatattcgag catctgacag caatagtaca
     4681 aaaatatatt tcaacaatat tgaaatacca ctaaaattaa ttgcagatct aattaatgag
     4741 ttaggaatta atgaattcat tgatagcaaa aaagagcttc aaatgctaag ttacaaccaa
     4801 gtaaataaaa taatcaattc aaacttcccc caacaagacc tatgttttca aacagaaaaa
     4861 cttttgttta cttcactctt tcaagaccct gcatttatat ctgcactaac atctgcattc
     4921 tggcaatctc ttcacatcac gtcttcctct gtggaacaca tatatgccca aattatgtcc
     4981 gaaaacatag aaaacaggct taatttcatg cctgaacaac gagttattaa caattgtgga
     5041 catattataa aaatcaacgc agttgtccct aaaaacgaca ctgcaatctc tgcctctggg
     5101 gggagggctt acgaagttag ctcatcaata ttaccatcgc acataacatg taatggtgtt
     5161 ggtattaata aaatagaaac aagctatctc gttcatgctg gaacacttcc aagcagtgaa
     5221 gggcttcgca atgctatccc tccagaaagt cgtcaagtat catttgccat catatctcct
     5281 gatgtttaag ccaatattat attggctgcc agactgtaaa aaaacgttct tcagaacagc
     5341 tctataaact attaaaccaa agctaaaagg atggggacca atagaatctc agcccatcct
     5401 ttttatatca ttatgaaccg ccccgg
//
LOCUS       DAJTRQ010000192         5412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000192 DAJTRQ010000000
VERSION     DAJTRQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..132)
                     /locus_tag="OO332_003903"
     CDS             complement(<1..132)
                     /locus_tag="OO332_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5944168.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     "
     gene            complement(143..376)
                     /locus_tag="OO332_003904"
     CDS             complement(143..376)
                     /locus_tag="OO332_003904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944169.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(373..1644)
                     /locus_tag="OO332_003905"
     CDS             complement(373..1644)
                     /locus_tag="OO332_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5944170.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            2038..3408
                     /locus_tag="OO332_003906"
     CDS             2038..3408
                     /locus_tag="OO332_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944171.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3590..5412
                     /locus_tag="OO332_003907"
                     /pseudo
     CDS             3590..5412
                     /locus_tag="OO332_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
BASE COUNT         1671 a         1128 c         1045 g         1568 t
ORIGIN      
        1 cagtgtattt ataacatcgg gagcagattt accaccgtat atgctgtcat acaattcatg
       61 cgctttctta cttgccacca cgcctgatga aactatcagc attaaagaaa ataatattct
      121 attaggcaac ataaaatatc tcttatttat cttgtacctg aatatgctct tcttttaata
      181 aatcacgttg ttttccattc agtatcgctg tcgcagattt aatagaataa gtcaaatcat
      241 gaaaatcgca taaattacga ttatacattg ggatgtctat atagcttttt cttatataac
      301 tggaattatt atacatatcc acaccgagcg tattcaaact aaaaggaggg acagaaagag
      361 tatattcttt actcactccc tccccccccc ttttagctca agcgtcaact gcaaattagt
      421 tatactacta attttatctg cattaatttc aatactgaca tcgcatattt tgggtttttc
      481 atttgcgtta aagccatagg tcccgtaatg ttttaacgtg atgtaaggta aagtatcacc
      541 ctgtgtttct cgggttttct gatcttgatt acaacgggtt aaatattcat ccttattgaa
      601 tttcattgaa tatttccccg cacctttttc gtaataatcc cataccttat cacaatcaag
      661 cgtttcacct aatccgttgt aatacatcca acttaaccac aatgcggctt tttttgcccc
      721 ttgctgttca gccttgtcaa aaagcatgct cgctttagaa taatcaacct caccggtcaa
      781 accagaatag tatatattcc ccagggccac taatgctttt tcgtcattct tatttgcagc
      841 taattcgtac cattttttgg cttcgacagc atcaatcttt aaaccagcac tgccattttc
      901 gtaggtatcg cccatggtat actgcgccat cggataacct ttttctgccg cagtacgaaa
      961 ccattgcagt gctttcttta tggtgtctaa agaacgtcta tcatcagaag gtgaaaaatc
     1021 atcgagataa agatttccga caatataatc catttcgggg tcgccttcct gcgcccatag
     1081 agtcgccaga cgcagaattt caggtttact gaaaccaatt cgcccccaaa aaagcaaatc
     1141 attctcgggt gcctgaccga cataacgact gacaaattca attaaactcg cccgtttcgt
     1201 tttgtctgtc tcagccccgg atttcaattc ataggcctga ttgattattt tattcatcaa
     1261 atcaggcgga atattaagat cgtcagtttt cgacttactg gcatacttct gtgctcgtgt
     1321 cacatctagg gtgacgcctt taccatattg aaagaaataa cgtgcattat aacaaccgta
     1381 ttcgcccccc tggttacagg ctttctcata ccactgaatc gcctttggga tgtcaacaga
     1441 tactgtcact ccgtgttcat aaataaatcc cagtagcact agcgcctgcg tgttattggt
     1501 atcagctaat tttttcagtt cttttaacgc cgtctgatct ttattgacaa tacgttcata
     1561 cagttgatca atatctgttg aagcatgaca aaaagaagcc agagataaac ccaatgataa
     1621 taaaataact ttaccagacc acatacattc ctccttgata tattcaacca ccaaacatcc
     1681 acctcatgaa taaatacgct gccataacgg cccaggtaat aagtaaacat ttaagctgaa
     1741 acagcataac cagtgcctcc aacgcttcta tttattgcga taaaataaca ccatcctttg
     1801 ataaaaagtc acacgcccat caataaatcc aaaaataatg tcattagttg caggataaaa
     1861 tcatttgagc ttttcatttg ttatatttaa cgcatgatgt attaatggca agaatatgag
     1921 agaaactcat acctgacgta aaaatcactc tcttatgtaa tatttactta ttaacactct
     1981 gtttacctaa catgcgctcc gcactacact atgtgggcaa ttttttaaga ggtggtaatg
     2041 aacgattata aaatgacgcc aggtgagcgg cgcgcgacct ggggtttagg gaccgtattt
     2101 tcattgcgca tgctgggcat gtttattgta ctgccggttc tgactacgta cggtatggca
     2161 ctgcaaggcg ccagcgaagc attaatcggt attgccattg gtatttatgg tctgacccag
     2221 gccgtttttc agattccgtt tggcctgctt tccgaccgca ttggtcgaaa accattaatt
     2281 gtcggtgggc tggcagtgtt tgccgccggt agcgttatcg ctgcgctttc tgactccatc
     2341 tggggaatta ttctgggccg ggcgctacaa ggctccggtg cgattgccgc tgccgttatg
     2401 gcgctgcttt ccgatctcac gcgcgaacaa aaccgtacca aagcaatggc gtttatcggc
     2461 gtgagctttg gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa
     2521 cttgggctgc acgcgctgtt ctggatgatc gctattctgg caacgaccgg cattgcgttg
     2581 accatttggg ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg
     2641 aaaggcagtt tcagtaaagt gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt
     2701 atgtgtctgc atattttgct gatgtcgacg tttgttgccc tgcccggaca actggctgat
     2761 gcggggttcc cggcggctga acactggaag gtctatctgg cgacaatgct aatcgccttt
     2821 ggctcggtcg tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt
     2881 gtcttctgcg tcgggttgat cgtggttgcg gaaattgtgt tgtggaacgc gcaaacgcag
     2941 ttctggcaac tggtggtcgg cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc
     3001 ctgctgccct cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt
     3061 gtttactcca ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt
     3121 gacggcatgt ttgacggtca gcgggtattt ctcgctggcg caatgctggc cgcagtgtgg
     3181 ctggcagtcg ccagtaccat gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc
     3241 ccagaaaacg taatggcaga tgaaaaactg gaaaaatgct tactgacaac gcctggtgtc
     3301 aaagatgtac tgctggcgaa gttagaacat tgcgtctatg tgaaagtaga cagtaaaatc
     3361 accaatcgtt ttgaaataga gcgagtaatc aacgaagtat cttattaaat aataagcacg
     3421 gtgctattca tgaaaaataa aatagcaccg tacttcgtaa caccataatc ctttaaaccc
     3481 tccattatta atacttcgct taattccata taactcgacg attacattag aagtaaacta
     3541 tactttttat gaaatatcag ggataaatta ttcacatgga aaatacatca tgtacaaatc
     3601 attatttact attatcattc tactgtatag cgctagttca tatgcaaaaa gcgaatcccc
     3661 tcccgcaagc actataactt tcattgcaca aatcgatcat gttgagctaa ataaaacagc
     3721 attatctcat gatatgaaac tcgttcttgc cgatcgtttt tatttcaaaa ccagtaatcc
     3781 ttgcgaaaca aattcaattt ctgctcgacc agaatcaatg gatattactg acaaagccta
     3841 tgccaacaaa ttaaaaaaat tagaaactcc ttttcttgat gatcgctatt tctttatgac
     3901 catcaatcag tgtgacggta atggcactcc catgctgaca gacataaaag tatgtacaac
     3961 agaattatgt ggtgctgaat atacaaagaa aatgtctact gtatggctcg atgaaacttt
     4021 aaaaccaact gctaaacgtc tggcaattta ttttattcaa tctcctttac cctatgacga
     4081 ggagaaaaaa caatggaaag tcaccggatg gtatatctca tcaactgata atcagtcttc
     4141 aactaaacag attgcatttg aaggatatac agacacagat aacttctccg attctaaatt
     4201 tatttcttca tttaaatcct ggtttgaaag tggaaagata aaaaacattg ctaactataa
     4261 tccaaaagga gaaagagatg gaaaatacga aagttattac aatagcaatg gtaacgtcta
     4321 tgaatctcta agtttttcta atggtcttat caacggtgag tttattacat attatgaaaa
     4381 tggggccatt gaaagtaaac gtcattttaa taacgacaaa attaaagatg gtgaatgccc
     4441 tcatttttat gaagatggga aaatcaaaca gcaacatagc tatctgaata gtaaactcga
     4501 tggtcctgca tatgaatatt atcctgatgg caaactcatg caggaaaatt tctatcagca
     4561 aagtgtgttg attggaaacg ataccagtta ctaccaatcc ggaaaaataa tctctataca
     4621 caatagaaat tcccgtggtc agtatgatgg agctaacgaa cgttatagtg aagagggtaa
     4681 gttacagtct aaatccgtct ataaagacgg aaaacaaatt tctgttcaaa cctggtatga
     4741 aaatgggcag aaagaagagg agaaacattt tgatgagcaa ggccaattaa atggtctggt
     4801 taagcaatgg tataaaaatg gtaatcttgc aaaatcacaa aactataaac atgacatact
     4861 cgatggtaac agtgaagagt ggtacgaaaa tggcattcct gaaagtttgt acccttataa
     4921 gaatggaaaa accgacggca ttgccaaatc atggaataag tatggtaaat taacctattc
     4981 aatcgaatac aaaaatagcg tagagaatgg ggcatatcgt aactggagca aaaacacagg
     5041 taaactaacc aaagaaaccc tgtacgtcaa tggtatcaga cagggagtgg aaaaagagtt
     5101 taacgatcgc acaggtaaac tattaacctc cacgcaatat gttaataata aaagacatgg
     5161 tacggaggaa acttatgatc aaaacggaat aaaatatatt acatgctatc aaaatgatca
     5221 gaaattatcg tctttagaca atccaacaca aataaaagat aatgcaacaa caggcgattc
     5281 cagtgctcaa tttgcattag ggaaatacga attcatctgt gcaaatatag atgaagggat
     5341 taaatggtta accaaatctg cagagcaaaa aaaacactga tgcgatatac tttttagcta
     5401 cagcctacaa ag
//
LOCUS       DAJTRQ010000193         5396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000193 DAJTRQ010000000
VERSION     DAJTRQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..794)
                     /locus_tag="OO332_003908"
     CDS             complement(<1..794)
                     /locus_tag="OO332_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5944172.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGA"
     gene            1040..1666
                     /gene="gstB"
                     /locus_tag="OO332_003909"
                     /pseudo
     CDS             1040..1666
                     /gene="gstB"
                     /locus_tag="OO332_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1663..2777)
                     /gene="yliI"
                     /locus_tag="OO332_003910"
                     /pseudo
     CDS             complement(1663..2777)
                     /gene="yliI"
                     /locus_tag="OO332_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2888..3271)
                     /gene="bssR"
                     /locus_tag="OO332_003911"
     CDS             complement(2888..3271)
                     /gene="bssR"
                     /locus_tag="OO332_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5944173.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3484..4809
                     /gene="rimO"
                     /locus_tag="OO332_003912"
     CDS             3484..4809
                     /gene="rimO"
                     /locus_tag="OO332_003912"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5944174.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4856..>5396)
                     /locus_tag="OO332_003913"
     CDS             complement(4856..>5396)
                     /locus_tag="OO332_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5944175.1"
                     /translation="AIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRK
                     SFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKV
                     YRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNKCTTVERLKML
                     ADERLYKSKKNGRAQISWQ"
BASE COUNT         1350 a         1397 c         1297 g         1352 t
ORIGIN      
        1 ttcgccccca gcactacgga gattaaacgc atatcaccct gggtagccga agcaaccaga
       61 ttatatcccg cacctgccgt ggttcctgtc ttcatgccat caacattcag attgctgctc
      121 cacagcagac ggttacggtt aggctgacga attttgttga aggtgaactc tttctcttta
      181 tgaatggcgt actcttccgg cacatcgtgg atcaatgctt tacccagcaa tgccatatcg
      241 cgcgcggtac tgaactgccc cggcgcatcc aggccgtgca ccgtctggaa ggtagtgttg
      301 gtcagcccca gttttttggc ataaccattc atcagaccaa taaaggactc ctggctcccg
      361 gcaacgtaat cggccagcgc aatacaggcg tcattaccgg actggataat cacacctttg
      421 ttcaagtctg ccaccgaaac ctgatcgccc ggtttgagga acattaccga tgaaccacgc
      481 agtgccggat ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc ggtgagttta
      541 atcttatcgg ccttaagcac ctgcccaacc acatagctgg tcatgatttt agtcaggctc
      601 gcgggatcca gtttctcatc cgcattgcct tctgccagca ctttaccgct ggcgtaatcc
      661 attaaaatcc atgcacgcgc atccacgctc ggcgcttcaa cggtttgttc ggccgcgaat
      721 gccgttgggg caaaaaggaa taaaaatgca gaacccgctg caagagcacg aaggagagag
      781 gagtattgcg tcataaatgc cacccgagta tccattcaaa aaaatacgtt atatcgccgt
      841 atgataagtt cggtgattaa taacgcacac acaaacttaa agaaaccgta agttggtaaa
      901 gtttttaaag tttatgcaat atcctgccgc ttcgctgctt ctggtgtcat ttttttgcca
      961 gctgttgctt aaaagttaac tttacttcac catcggagtc ctgttcagtc tgtggctgcc
     1021 tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg aataattcaa ctaacgtaaa
     1081 aaaagtattg ctgacgctcg aataactgga actaccttat gagcaaattc tcgcgggccg
     1141 tgagtttggg ataaatcacg atgctgattt tctggcgatg aaccctaacg ggctggtgcc
     1201 gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa tcaaacgcca ttgtccgcta
     1261 tctggcggca cagtacgggc aaaaacgcct gtggatcgac tcaccggcac gtcgtgcgga
     1321 agcggaaaaa tggatggact gggcaaacca gacgctcagt aatgctcatc gcgggatcct
     1381 gatgggatta gtcagaacac caccggaaga gcgcgatcag gccgccattg atgccagttg
     1441 caaagagtgc gacgccctgt ttgccctgct cgatgcggaa ctggcaaaag taaaatggtt
     1501 ctctggcgac gagtttggtg tgggcgatat cgctatcgca ccgttcatct acaatttgtt
     1561 taacgttggc ctgacctgga caccgcgtcc aaatctgcaa cgctggtatc agcaactcac
     1621 tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt agctaattgc gtgggctaac
     1681 tttaagtaat tccccactgg actcgtcggt gagaacgtat aaataaccgt cgggtccagt
     1741 gcgaacatca cgaattcgct gccctctgtc cgttaaaata cggccatctt ctgtcacttt
     1801 gtcgccgttg acgctcatca caatgacatc tttatctttc agcgcccaat aaataatttt
     1861 tgctgccact gggggaattt atcgctgtta tagaaggcca tgccgctcac agcgggcgaa
     1921 tctttccagt aaaaaacagg ttgctcggtc ccggcgacga tctccccttt cgcttccggt
     1981 atcttaaagc ctgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct
     2041 ttttgcggga tattaatttc atcaccaccg cgcgggccat gttcattcag ccacagtgca
     2101 ttactccacg gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg
     2161 ccgcgcgcac cggattcctt tataaaagga ttatcatccg ggatttcgcc ctggtcggtc
     2221 agacgcacca gtttgccctg taatttatcc agatcctgcg ccgtcgggcg ctgattgttt
     2281 tcgcccagag caataaaaag ataaccttta ccgtcgaata ccagccgccc gccaaaatgg
     2341 ttgccggtag acagttttgg catctggcgg aagacggtgc ggaagtcggt cacttttgag
     2401 agatcatcac ttaagcggcc ataacccaca gcagttccgg ctttgccatc atcgccaact
     2461 tcggaataac ttaaccagat gcggcgagac tgagcaaaat caggcgctaa aaccacgtcc
     2521 agcaggccgc cctgcccgtg cgcccaaacg tccggaactc cggaaagcgg cgcagataat
     2581 ccttttcctg cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga
     2641 ttatcgggta aaaaggccag tgcccaggga tggtcgagtt tgtcttgcag tacttcgaca
     2701 tttaccgttg caggagccgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa
     2761 aaggattgtc gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag
     2821 gagataacaa attcattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc
     2881 ttagttttca ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta
     2941 agctcatcag acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat
     3001 ggtgcccact catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag
     3061 cactccactc ttcctgatcc agatgaagat tcaaccgcag cgatttatcg tgcagttcgc
     3121 gattcagttt aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt
     3181 ttgccttcct cagttgcacg tatgcagcga ggtcaacgcg tgcgtcgatc aaccggttca
     3241 gcagatcgat tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt
     3301 gcaattaaat agtatggcta ttttttgagc aaaatgctgt ctaggatcaa ttcttcacgc
     3361 cgaattaaca aaatttacat ttccttcgcc tccgccagcg aatgagattg cgatacaaat
     3421 tttatccaga actctgtaga atccctgccc tgacctttcc tgtaactgaa tattttttaa
     3481 gctatgagca aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa
     3541 aaccttgtcg attcagagcg tattctcacc gaactccgca ctgaaggtta tgacgtggta
     3601 ccgagctatg acgatgcgga catggtgatc gtcaacacct gcggctttat tgacagcgcg
     3661 gtacaagaat cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg
     3721 accggttgtc tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa
     3781 atcaccgggc ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa
     3841 ccgaaacaca acccattcct gagcctggtg ccagaacaag gtgtgaaact gacgccgcgt
     3901 cattatgcct atctgaaaat ttcggaaggc tgtaatcacc gctgcacctt ctgcattatt
     3961 ccgtctatgc gcggcgacct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa
     4021 cgtctggtag atgcgggcgt taaagagatt ctggtgatct cgcaggatac ttccgcctat
     4081 ggcgttgatg ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg
     4141 gtcagcctgt gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctacgtttac
     4201 ccttatccgc atgtggacga cgtcatccca ctgatggcag aaggcaaaat cctgccgtat
     4261 ctggacattc cgttgcagca cgccagcccg cgcattctca aactgatgaa gcgtccgggt
     4321 tctgtagatc gccaactggc gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc
     4381 ctacgctcaa cctttattgt cggcttccct ggcgagacgg aagaagattt ccagatgcta
     4441 ctcgacttcc tgaaagaagc acgtctggat cgcgttggct gctttaaata cagcccggtt
     4501 gaaggtgcag acgccaatgc cctgcctgac caggttccgg aagaagtgaa agaagaacgc
     4561 tggaaccgtt tcatgcagct gcagcagcag atttccgccg agcgcctgca agagaaagtg
     4621 ggccgtgaaa ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc
     4681 atggcagatg caccggaaat cgacggcgcg gtttatctca atggtgaaac caacattaag
     4741 ccgggtgata tcctgcgtgt gaaagtcgag cacgccgatg agtacgattt gtggggtagc
     4801 cgggtttaat taattcaggc cgggcaaaat catttgtccg gcctgcgaat aatgattact
     4861 gccagctaat ctgtgccctg ccgtttttct tactcttata cagccgctca tccgccagca
     4921 ttttcaagcg ttcgacggta gtacatttat ttgaatgcgc tacaccgata ctggtgctta
     4981 aattaatgct tcccacctgg aaatgaacga tttcttttag tatcgatagc aactcttcca
     5041 gcgtgccgcc agtataaatc accgcaaatt cgtcacctcc gaagcggtag actttacctt
     5101 tatctaccgg gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca
     5161 gcaaatgacc gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac agacacaaat
     5221 aaccgccttt ttccggcagc gcttttaaat cttcatcaaa ggattttctg cccattgctt
     5281 tggtcaggtc gtccaggaac gccgagttat acatactgta gagttttttg acatcggcag
     5341 ccgaaaagaa aagcccgcca tccagtacgc gttcaaaatg gttattaatg atggct
//
LOCUS       DAJTRQ010000194         5347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000194 DAJTRQ010000000
VERSION     DAJTRQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..158
                     /locus_tag="OO332_003914"
     CDS             <1..158
                     /locus_tag="OO332_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5944176.1"
                     /translation="RGALLYGLIGSCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPW
                     NVVLTDK"
     gene            190..804
                     /gene="istB"
                     /locus_tag="OO332_003915"
                     /pseudo
     CDS             190..804
                     /gene="istB"
                     /locus_tag="OO332_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(973..5067)
                     /gene="sepA"
                     /locus_tag="OO332_003916"
     CDS             complement(973..5067)
                     /gene="sepA"
                     /locus_tag="OO332_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5944177.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1310 a         1350 c         1251 g         1436 t
ORIGIN      
        1 atcgcggagc tctgctgtac ggactaatcg ggagctgtcg gttgaacggt atcgatccag
       61 aagcgtatct gcgccatatc ctgagcgtac tgccggaatg gccttccaac cgagttgacg
      121 aactgctgcc atggaacgta gttctcaccg ataaataagc gtcaatacgg ccttccgttg
      181 acgcttacag aactcctgtg gcgcgagagt gaaaaactgc gtcgttataa aaaggaggcc
      241 cggttgccag ttgccaaaac gctaagcgaa tacgacttca ttcagttgcc ggaactgaat
      301 ggagctcagt tccagcaact ctgtgaaacg acagactggg ttgatgctgg agaaaacgtt
      361 ctgctgttcg gggacagcgg gttggggaaa agccatctgg cggcagcgat cgtggatggc
      421 gtagtaggcc agggctaccg ggcccggttc aacagcgcag gagagctgtt gcaggaacta
      481 cataaagcca gagctcagtt gaaactgaat gagctgctac tgaaactgga tcgctaccgg
      541 gtgatagtgg tggatgatct tggctatgtc aaacgcgaca gcgccgaaac gggtgtgctg
      601 ttcgagttaa tagcgcatcg ctatgaacgt gggagcctgg tgataaccag taaccatccg
      661 ttcagcatgt ggggcagcat cttcgtggat gagactatgg cggtggcggc agcagaccga
      721 ctgatccatc acggatatat gttcgaactg aaaggggaaa gctacaggaa aaagacagcg
      781 aaggcagtaa caagcgtgac ttgatgtcgc actgaagggg cggccagtat agttggcgcc
      841 agtcggcaaa actagttgac gtctaatacc tggacggtca cttctgagaa aaggcattta
      901 cacagaatct taaacaggct cacggagttc tttcagaaaa aggcctgccg aatggcaggc
      961 ctatcccatt cgtcagaaaa cataacggaa gttggcgttt atcgcattat ccacattata
     1021 tttcccgaac gctgatttct ccagctccag tcccaaacgc atattatcct taatttccgc
     1081 attcatccct accgtcatca gcatcctgct atctttttca ccttcgaaac gtttctctcc
     1141 ggaagcatcc tgcagaaccg tttctccgtt cgccagcagg tcgaactgat aacccagccc
     1201 ggctcgagct gtgattttcc agtcgtctcc ggagaaggct cttcccacgt caacaccagt
     1261 acggccaatc agtgggttat aatccttgtc tttcatgctc agagccattc cccggtcttc
     1321 ccagctaaaa gcttttcctg atacagaacc gtaaaccagc tctatctgtg gctccaccca
     1381 ggactctttc gtcaggtggt aacgataacc aacttcagca ccggtatacc aggaatgaga
     1441 gctgtagtct tttgttccca gtgaggcaaa attcgccgtg tgctgattat catgatggag
     1501 atatttacca atcaggtcaa aataagctcc ggaattaaag agtgcagagg catacagacc
     1561 gccacccacg gatttgtttt ttccactgaa tgcgtggctg cttgcattgc tgtccgtata
     1621 ggtcagcaat gcccccgtaa ataaatccac accgtccagc tcatgctttc tgtcgacacc
     1681 aatctgaacg tgagtgtagt tatcgctgta gtcaccatct gccgaaccgg taccattcat
     1741 tatgcgtgcc cagacaccgg catccccctg agtatcccgc aggtcaccca tacgtttgtt
     1801 caggttattg acctctgtaa ggaaggtctt ataacctgat gccataaagt cttttgcggc
     1861 tttcgaggct ccggcatcag cggtagtctg atatcctgtc agcacccatt ttgtggcatc
     1921 atccgttttt tccgtgctga ttactggcgt aacgttactg aaaccaatct gctgcgtacc
     1981 ggcagtgaac gttttctcat cactcccttt cggtgcggta attaacgtca cgctcatttc
     2041 actggctggt ttgtttaaaa agtcaaccag gacgctatta ttactgcctg acagtttgtc
     2101 tgtgacattc aactggtcag cccggctgtt gttggtatgc atcaggaacg tggtattatc
     2161 tgcagaaagc tcttttactt gtaaagtggc aaagtgtttc ccgtccccca gttcaattcg
     2221 cgagccctta gccttcagtg caccggcctc tgattttccg tcagagaacc acagagcatt
     2281 gatcatattt accgtactct gtgcccccgt aatactgcct ctcagaacag cgttatatcc
     2341 cttcattaag gagctgaata acggagagtc cgtcttccct gcatcagcat cactgtctcc
     2401 cagattaatg gttgccgcag tggttcctgc atgaatatcc gaactcagat agcccatatt
     2461 tttaacagaa aagctggcgt tatcttcgag gttaatccct tctgttgtag aaattacggg
     2521 tgagtaatac ccctgtttct gcgcagaagg catccccgtc agggtcagag aagcattttc
     2581 ttttacatcc acagccccgg tgctgaaaat accgctctga gccgtaagtt ttgctccttt
     2641 tccgagggtc aggctgctgt tgacaaagct gccaacacgg tcaaaaacag cattctgaga
     2701 ggtcacactc acggtgctat cataagcctc aataccaccg gtgaatctct ccatgatttg
     2761 cagggatgat ttattttcca gcgtaacatt tccgctgtaa tcactctggt caccaactgt
     2821 ggttgcagtg gcactccctt caacagagga gaccgcatta ccggttccgt cactcctgtc
     2881 agtaaagaca cggctacttc ccagaatgac tgtacttcca ttggtggcgt taatatctcc
     2941 ctgcatcttg ccgtttctgg acagatggaa tgttgccgca tcgagtttca gtttttccag
     3001 cgtaaactga cgggtttccc agtcgctctg gctggaagaa gtcgtcgttc cagcatgaat
     3061 aactggatgc ccctggaata tcagcgtgcc ggacttcttg gttatcgaac cttctggcag
     3121 gttaactgaa ccatcaagag ccagtacatc cttacctgag agctgcggaa tattcacatc
     3181 catattgccg tttagttggc cgtgatacat ataaggctgg ctgctggcct caattttttg
     3241 ttgcttcaca gtatcaatgg ctttattacg atcttttccg acattctgcc agacactgct
     3301 gttgttcaga tcggagaaga acggggaata tgaactcgct ttcaggatga aatattgctt
     3361 ggtagaactg tcataatata ggtctcccgg cgctcctctc cccccaaaaa tactgacagt
     3421 attaaccggt acattgatgt cactggcttt taatgtctga aggtcgagtg ttaccgttga
     3481 ttttttctca ctgttattgg taatgatggc cccgtagtca gccgcctgaa gacgggtaaa
     3541 ggtcagatta ttaccattca gttccagacg tcctccacgg tatccccacg aaatattatc
     3601 cggattaact tgctgtgagt cagaaagcac aactgtcgga cgaccgctgg caataccaac
     3661 agagctgaaa gcctgtacct ttccgtcagc atccgcctgc tggttgagaa taacggtacc
     3721 gtcccccacc ttgagtccgc ctgcattcac accggtcccg tttactgtca gggtaccttc
     3781 gccagtttta tgcaggttat caccggcaac tccgttcacc tgccacagaa cgtgggttcc
     3841 tttatcggtg ataatcccgc cccccatcca tgtctggccg ttcagggcgg acaccctgta
     3901 atcgccggca aactgcagat acccagctcc ctgattaacg gaattctgca gaacgacctc
     3961 gccattattg ccggtgaaca ggaggttttt tcctgcgttc agatcatttc ctttctttcc
     4021 atgcatatcc caagtcgtgt ttccctgagt cagtgtgccg gtgccattag ccgcatcata
     4081 tttccactgc agtactcctt caccagaggt gtaggctata gttttgtcaa aatcattttg
     4141 cggttgctga ccaaggaaat cctgagtggt gacgacccag ttatttccat agttagttcc
     4201 tgaggaaagc acgccaatca gtacccattt tttttgcagg gaatcataag caaacagggg
     4261 ggagccgctg tcccccatat tcagataatt agccagaggt ccattcgcgg gattaaaaat
     4321 gtcaccggtc tgagctgtaa tcatctgtcc gttgttatag gagttcagcg cgctgacggt
     4381 tcccccaaca agaaatccgc cattggtata cacttgggtg cgattaccat ttttgtcttt
     4441 gacgtactgc atcccccccc caaggcgata gaattcagta aagcgtccgc cggcctgata
     4501 ggcaccgctc acagccccga tgtcagacac ttctgccggc gccacctctg tcaccagttt
     4561 actcaggcgg cgggttttaa tatcaagacc gctattatta tttgtaccaa ccgcagtata
     4621 ggtgtttttg gcgtatccga agctcatcgt gtcagaaccg ctgacatgtt tggcggtaac
     4681 cacatactga ggggagtaaa gtgtatggtc tcccggaggc aggctaccgg tagtaatagt
     4741 ggcactactg aaatcagcca tcggggcttt atcaagttta ccgaccagat tgccctgctt
     4801 gtcgtaaatg gaaatattcg tggtccccga cgtaaactga cctttattct cggcaaagtc
     4861 gcggaatatc tgataaggga tctctgcact gaccgttgct gacagagctg gccatgccga
     4921 agaaagtgat aaagctgcaa cagacgtaag aataacccga cgtgaaagtc tgcgatggct
     4981 tttgcaggta accctgcggg ccagttcgga gacagcaatc aggcttttgg ttatatggca
     5041 atacttaaga taatatattt tgttcatata gaaacatcaa cctaattttg acaaagatta
     5101 attacatagg atgtagatgg agcagtccga gattctacta atatcaatta ttaagcatct
     5161 gttttttagt tggtgtaata taatttttat taaggtaagc ggaaaaacaa ccaaaaaaaa
     5221 cgcaagatgt tattttttta tcattttgag ccagtttggc tggggggatg ctcttctctt
     5281 aagcttcttg ctcaacttta aaaatagtta tgccagttcg gtatcgataa gggccagttt
     5341 ggatatc
//
LOCUS       DAJTRQ010000195         5338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000195 DAJTRQ010000000
VERSION     DAJTRQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO332_003917"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO332_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5944178.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO332_003918"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO332_003918"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5944179.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO332_003919"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO332_003919"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5944180.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO332_003920"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO332_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5944181.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1457 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgactttc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgggg
//
LOCUS       DAJTRQ010000196         5292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000196 DAJTRQ010000000
VERSION     DAJTRQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..591
                     /locus_tag="OO332_003921"
     CDS             <1..591
                     /locus_tag="OO332_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5944182.1"
                     /translation="LTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARI
                     HMYKIATLGSGNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVY
                     CSSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTV
                     NKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            925..3204
                     /gene="ydeP"
                     /locus_tag="OO332_003922"
     CDS             925..3204
                     /gene="ydeP"
                     /locus_tag="OO332_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5944183.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3452..3649
                     /gene="safA"
                     /locus_tag="OO332_003923"
     CDS             3452..3649
                     /gene="safA"
                     /locus_tag="OO332_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5944184.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3723..4484
                     /gene="ydeO"
                     /locus_tag="OO332_003924"
     CDS             3723..4484
                     /gene="ydeO"
                     /locus_tag="OO332_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5944185.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4886..>5292
                     /locus_tag="OO332_003925"
     CDS             4886..>5292
                     /locus_tag="OO332_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR5944186.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1530 a         1126 c         1170 g         1466 t
ORIGIN      
        1 ttgacgacaa ataccaacgt attggatatt ggtgataaga caccgatgcc attaccgtta
       61 aaactctata ttaccccagt tggggcggcc ggtggagttg tcataaaagc gggggaagtc
      121 attgcccgta ttcatatgta taaaattgca acattaggaa gcggaaatcc gcgtaatttt
      181 acgtgtaata ttatttcaaa taacagtgtt gttatgccaa caggcggctg caccgttgat
      241 agtcgtaatg tcaccgtgga tctcccggac tttcctggaa gcgcagaaat accccttggc
      301 gtatattgct caagcgaaca aaagctctca ttttatcttt cgggtgccac cactgacagt
      361 agccgacaag tgtttgctaa cacagcacct gatgcaacaa aagcatcggg agtgggtgtc
      421 actctaatgc gtaatggaaa aatactggct acgggagaaa atgtctccct ggggacagta
      481 aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg aaacaaggtt
      541 tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata attgtgattt
      601 tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt tgtgaaggct
      661 attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt attttttata
      721 ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc gtatttaatc
      781 tattatttaa cgataataga ttaaaaatag aaagagagta gtcccctact cttatatatc
      841 catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa atcctaataa
      901 ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca gggtgctgca
      961 ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat ggatatacgc
     1021 catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg tccgggttgt
     1081 gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg cgcaaaagca
     1141 atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga gaatacggtt
     1201 caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct cactcagcct
     1261 ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca agctttcgac
     1321 gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt ctatacttcg
     1381 ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga atacgggagc
     1441 aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg tttggcagcg
     1501 agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg cgatttagtc
     1561 atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc gttgcgcgct
     1621 ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg tggcctggag
     1681 cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac tcagttggcc
     1741 agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg gatgatgcgc
     1801 ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt gcttgatgac
     1861 gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt tctcaattcc
     1921 gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc cgaactggct
     1981 gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat cactcagcac
     2041 gaacatggta cccagaacgt gcagcaactg gtcaatttgc tgttgatgaa aggtaacatt
     2101 ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca gggcgaccga
     2161 accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg tgagcgctat
     2221 ggcttccccc cacctcatgc acctggacat gctgcaattg ccagcatgca agcaatatgt
     2281 acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc aatgccagat
     2341 cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt agccactaag
     2401 cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt tctggggcgt
     2461 agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga ttcaatgtcg
     2521 atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa atcagagtgt
     2581 gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc ctgggagtat
     2641 ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc agagttcgcc
     2701 gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc agctgctgaa
     2761 aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg gctgttagaa
     2821 gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag ccacgatcag
     2881 tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg tcaacgagat
     2941 gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg cgaaagagtt
     3001 aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga tagattaaaa
     3061 gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga atcgaatcac
     3121 atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa aagtattccg
     3181 gttgaattag aaccatcaaa ttaatgcctc ttctcatttc ttctgctgtc atccgcacag
     3241 cagaagaatt cctcattgac tattattgcg caatttactc acatggatta aattaaacta
     3301 catactataa gatataaact tctgcctaca gctgtaagaa actccgctca gtactgaagc
     3361 accagtccta tttcttcttt tctccagcct gttatattaa gcatactgat taacgacttt
     3421 taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga aaaacaaaat
     3481 catgcaaaga gataactata aagaaatcat gtctgcaatt gtggttgtct tattactgac
     3541 acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag aaatgggtcg
     3601 cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa aataaacagc
     3661 aaattataaa tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt taaggagata
     3721 aaatgtcgct cgtttgttct gttatattta ttcatcatgc tttcaacgct aacattttag
     3781 ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct gttcgtacac
     3841 attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat accattaaaa
     3901 aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg aagtttttac
     3961 gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc aactcggaag
     4021 aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct atttttgccg
     4081 catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc gggaaagtga
     4141 gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt tgtgactgcc
     4201 tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg acattctcac
     4261 agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta gaaggttcag
     4321 tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt tatgcgttcc
     4381 gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt caaaggcaca
     4441 cgggtatgaa tacgggcaac acgatgagtg ctttagctat ttgattattt gctaacgagt
     4501 agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc cccgcgttgt
     4561 ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt tatgcacttg
     4621 cataacctgt tgcatgatta tttataatct caattcggca ttttgtcagt aaaatgcaat
     4681 aatttattaa atatcaataa attagttatt tatcggcgag aaattactta atagaacaga
     4741 aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat ctgctatctc
     4801 tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta cttcggaatt
     4861 aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg tcgtaagtac
     4921 ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg cggcagatga
     4981 tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc cgactgaata
     5041 cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg gttatggaca
     5101 acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg aagttgtcga
     5161 tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa cgccgacgct
     5221 cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac acggtgtttc
     5281 cggcccctcc cg
//
LOCUS       DAJTRQ010000197         5126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000197 DAJTRQ010000000
VERSION     DAJTRQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..236
                     /locus_tag="OO332_003926"
     CDS             <1..236
                     /locus_tag="OO332_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944187.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="OO332_003927"
                     /pseudo
     CDS             276..816
                     /locus_tag="OO332_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="OO332_003928"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="OO332_003928"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5944188.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="OO332_003929"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="OO332_003929"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5944189.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO332_003930"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO332_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5944190.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="OO332_003931"
     CDS             3834..4751
                     /locus_tag="OO332_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5944191.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="OO332_003932"
     CDS             4830..5012
                     /locus_tag="OO332_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5944192.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1228 c         1462 g         1184 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctagcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gctagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg gacgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DAJTRQ010000198         5017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000198 DAJTRQ010000000
VERSION     DAJTRQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(56..1561)
                     /gene="glpD"
                     /locus_tag="OO332_003933"
     CDS             complement(56..1561)
                     /gene="glpD"
                     /locus_tag="OO332_003933"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5944193.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            1751..2077
                     /gene="glpE"
                     /locus_tag="OO332_003934"
     CDS             1751..2077
                     /gene="glpE"
                     /locus_tag="OO332_003934"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5944194.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2122..2952
                     /gene="glpG"
                     /locus_tag="OO332_003935"
     CDS             2122..2952
                     /gene="glpG"
                     /locus_tag="OO332_003935"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5944195.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            2969..3727
                     /locus_tag="OO332_003936"
     CDS             2969..3727
                     /locus_tag="OO332_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5944196.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3709..>5017)
                     /gene="rtcR"
                     /locus_tag="OO332_003937"
     CDS             complement(3709..>5017)
                     /gene="rtcR"
                     /locus_tag="OO332_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5944197.1"
                     /translation="GYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSST
                     PRKKEQPCGPGEVTIIDLDLSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQ
                     IEKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMS
                     TLFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFG
                     SDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLD
                     YEVERHATLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRI
                     TLDVVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSA
                     AGRQLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1103 a         1351 c         1371 g         1192 t
ORIGIN      
        1 gtttgtcatc agtctcaggc cagattgaaa tctgacctga tcaccttacg ttaatttacg
       61 acgccagcga caacttctgc tgcgtatatt ccaccagcca ctgactcaca cgagattgtt
      121 gatccgcatt cagccacatg ccttgttttg tgcgacgcca cagggcatcg tcggtgcggc
      181 ggacccattc gtgatccacc aggtatttca gttccgcttc gtagaactca tgaccgaaat
      241 cttccccgag atcgcttacc gttcccgcat tgccgagcag cagctcgctg ttgctgccgt
      301 aagtgcgcgc gtagtgacgt gccagcgatt cagtcaggaa cgggtaacgg cggcgcaggc
      361 gagcggcgta atcgtcgcgg tcgccttcaa tggcaccacc cggcagcacg ctctctttcg
      421 tccacgccgg gccaataccc tgataatacg gcgtcagttt ttccagcgca tgttctgcca
      481 gttttcggta ggtggtcagc ttaccgccga ataccgacag cagcggtgct ttgccgtttt
      541 catcatggat atcaagggtg taatcacggg taatggcttg cggcgagtcg gactcatcat
      601 cacacagcgg acgcacaccg gagtaggtcc agacgatatc gtcacggctt agctgctttt
      661 taaagtgcgc gttatacact ttcagcaggt aattgatttc actctcttca atcttcactg
      721 ctttcggatc acctttgtat tccacatcgg tggtgccgat gatggaaaac tcgtccatcc
      781 acgggatcac gaacacaatg cgtttatctt cgttttgcag aatgtaggcc tgcttttggg
      841 tatgcacgcg cggcaccaca atatggctgc ctttgatcag gcgaatgcca taaggcgaag
      901 gcagatgcat cccctcgtcg aagaactgtt tcacccacgg gccggtggcg ttaaccaggc
      961 cgcgcgcttg ccagctatat tttttgccgg tatcgatatc ttccgcttcc acaatccaca
     1021 ggccgttttc gcggcgagca gaggtggcgc gagtccgagt aagcacttcg ccgcctttac
     1081 gcaccaccat ctgggcgttg gcgagtacca gacgggcgtc gtctacccaa cagtcagaat
     1141 attcgaatcc gcgcttaatt tccggtttta acactgaatt tgcgccaaaa cgcaaaccaa
     1201 ttgatcccgg caggctggtg cgtttaccca gatgatcgta cataaacaga ccaatgcgaa
     1261 tcatccacgc cgggcgcaga tgcggacgat gtggcaggcg aaaacgcatc gggaaggcga
     1321 tatgcggagc cattttcagc agcacttcac gttcagccag cgcctcgctg accaggcgga
     1381 attcatagtg ctcaaggtag cgcaggccac cgtgaatgag ttttgaactg gcggaagagg
     1441 tcgcgcaagc gagatcctgc gcctccagca tcagcacgga taaaccgcgt ccagcggcgt
     1501 ctgccgcgat accagcacca ttgatgccgc cacctatcac aatcagatct ttggtttcca
     1561 tgctgccctc attcactttc gttaaagctc ataaatgttc gttatcgaac atattagcaa
     1621 agaatcgcgc tttgggtaac attgaaaaaa cattttagag tgatatgtat aacattatgg
     1681 cgtttatctg ccgcttcgac gtaaactgtg cggtaaattt gcccacttgt ttgtaaagaa
     1741 agagagacgc atggatcagt tcgaatgtat taacgttgcc gacgcgcacc agaagttgca
     1801 ggaaaaagag gcggtgctgg tcgatattcg cgatccacag agtttcgcta tggggcatgc
     1861 ggtgcaggct ttccatttaa ccaacgacac gctgggcgcg tttatgcgtg ataacgactt
     1921 tgacactccg gtgatggtga tgtgttatca cggcaatagc agcaaagggg cggcgcagta
     1981 tctgctgcaa cagggctacg atgtggtcta tagcattgac ggcggctttg aagcctggca
     2041 gcgacagttt cccgcagagg tggcgtacgg cgcgtaacgc tttatactgt ccccttttgt
     2101 gtggaataag cgacagcaac gatgttgatg attacctctt ttgctaaccc ccgcgtggcg
     2161 caggcgtttg ttgattacat ggcgacgcag ggtgttatcc tcacgattca acaacataac
     2221 caaagcgatg tctggctggc ggatgagtcc caggccgagc gcgtgcgggc ggagctggcg
     2281 cgttttctcg aaaacccggc agatccgcgt tatctggcgg ccagctggct ggcaggccat
     2341 accggcagtg gcctgcatta tcgccgttat cctttctttg ctgccttgcg tgaacgcgca
     2401 ggtccggtga cctgggtgat gatgatcgcc tgcgtggtgg tgtttattgc catgcaaatt
     2461 ctcggcgatc aggaagtgat gttatggctg gcctggccat tcgatccaac actgaaattt
     2521 gagttctggc gttacttcac ccacgcgtta atgcacttct cgctgatgca tatcctcttt
     2581 aacctgcttt ggtggtggta tctcggcggc gcggtggaaa aacgcctcgg tagcggtaag
     2641 ctaattgtca ttacgcttat cagcgccctg ttaagcggct atgtgcagca aaaattcagc
     2701 gggccgtggt ttggcgggct ttctggcgtg gtgtatgcgc tgatgggcta cgtctggcta
     2761 cgtggcgaac gcgatccgca aagtggcatt tacctgcaac gtgggttaat tatctttgcg
     2821 ctgatctgga ttgtcgccgg atggtttgat ttgtttggga tgtcgatggc gaacggagca
     2881 cacatcgccg gattagccgt gggtttagcg atggcttttg ttgattcgct caatgcgcga
     2941 aaacgaaaat aattccaggg atttataaat gaaacaaaca caacgtcaca acggtattat
     3001 cgaactggtt aaacagcagg gttatgtcag taccgaggaa ctggtggagc acttctccgt
     3061 cagcccgcag actattcgcc gcgacctcaa cgagctggca gagcaaaacc tgatcctgcg
     3121 ccatcatggc ggcgcggcgc tgccttccag ttcggttaac acgccgtggc acgatcgtaa
     3181 agccacccag accgaagaaa aagagcgcat cgcccgcaaa gtggcggagc aaatccccaa
     3241 tggctcgacg ctgtttatcg atatcggcac cacgccggaa gcggtggcgc acgcgctgct
     3301 caatcacagc aatttgcgca ttgtcaccaa caatctcaac gttgctaaca cgttgatggt
     3361 aaaagaagat ttccgcatta ttctcgccgg tggcgaatta cgtagccgcg atggcgggat
     3421 cattggcgaa gcgacgctcg attttatctc ccagttccgc cttgatttcg gcattctggg
     3481 gatcagcggc atcgacagcg acggctcgct gctggagttc gattaccacg aagttcgtac
     3541 caagcgcgcc attattgaga actcgcgcca cgttatgctg gtggttgacc attcaaaatt
     3601 tggccgtaat gcgatggtca atatggggag catcagcatg gttgatgccg tctacaccga
     3661 cgccccgccg ccagtaagcg tgatgcaggt gctgacggac caccatattc aactggagct
     3721 gtgctgatcc tgcacggcct cccacgtcag accaaaacgc gccaggtatt tgcgtagccg
     3781 atccgcgtca ttgacgctgg ctttgccctg gcgcgaaaca tcaaaaagct gtcgtccggc
     3841 agcagaaagc gactttgcct ggcggcagat agcgataacg tgttccagtt gcatacggtc
     3901 gaagagatcg atgttttctg cctctgcgcc cagcaacgcc gtaagcgcgg aggggcggct
     3961 ctcctgccag ttatagcgca gacggtttat ctcatcttcc accacgtcca gagtgatgcg
     4021 tccgctagtg gcaaaggtgg ccatccgcgt gacgctggca gaaagttcgc gaaagttacc
     4081 gcgccatgta gcctggggag atgttgcaaa ggccagccag gcacgccgcg cttcggtgtt
     4141 aaaacgcacg ctgtcaccag tgagtgtggc gtggcgctcc acttcataat ccaggttcgg
     4201 ttcaatatct tcctggcgct ggcgtagacc cggcagggtg aaggtccaga gattgatccg
     4261 cgcgtacaga tcttcgcgga atttgccttc ggcaaccagc tggcgcaaat cgcgcaccgt
     4321 tccggcgata agctgaaaat cactgctcac ctggcgatcg ctgccaaacg ggtaaaaggt
     4381 tttctcttca atagctttca gcagcattgc ctgttcgtct gcgcccagtt cgccaatctc
     4441 atcaagaaac aacattccgc cgttggcgct gcgtaataat ccttcacgag attcccgcgc
     4501 cccggtaaac gcgcctttca catgaccaaa cagcgtcgac atcgcggtat cgccgcgcag
     4561 ggtggcgcag ttcacttcca caaacgcgcc gctaaactga tgccgtgcct gttttaactc
     4621 gaagatgcgt cgcgccagaa atgacttacc tgcgccggtc gggccgttaa gcagaatcgg
     4681 cgcgcgggat ttaatcgcca ctttttcgat ctgctcaatc atgcggttga agtgggggtt
     4741 acgcgtggca ataccggatt tgaggaaatc gagcgtttgc tgccgttcct cggcaaagcg
     4801 gctggcgatg gcgttataac ggcttaaatc gagatcgata atcgtcactt cacctgggcc
     4861 gcacggctgt tcttttttgc gcggagttga agattgtatc aaccgggcgg gtagataacg
     4921 cgcttccgcc agcagaaacc agcaaatctg cgcgacgtgg gtgccggtgg tgatgtgaat
     4981 taaatagtct tctttttctg gctgaaactc gtaacca
//
LOCUS       DAJTRQ010000199         4984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000199 DAJTRQ010000000
VERSION     DAJTRQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.202"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..181)
                     /locus_tag="OO332_003938"
     CDS             complement(<1..181)
                     /locus_tag="OO332_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR5944198.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
     gene            complement(245..2074)
                     /gene="recQ"
                     /locus_tag="OO332_003939"
     CDS             complement(245..2074)
                     /gene="recQ"
                     /locus_tag="OO332_003939"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5944199.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="OO332_003940"
     CDS             complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="OO332_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5944200.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3241..3708
                     /locus_tag="OO332_003941"
     CDS             3241..3708
                     /locus_tag="OO332_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5944201.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3760..4668
                     /gene="rarD"
                     /locus_tag="OO332_003942"
     CDS             3760..4668
                     /gene="rarD"
                     /locus_tag="OO332_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5944202.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
     gene            4709..>4984
                     /locus_tag="OO332_003943"
     CDS             4709..>4984
                     /locus_tag="OO332_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944203.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTL"
BASE COUNT         1185 a         1286 c         1241 g         1272 t
ORIGIN      
        1 gcaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc
       61 ccatcatcgc ttctttacgg gaacgactga cagcggtctg agagacaaaa aagaaatcgg
      121 gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgagaaat aacatcaaca
      181 tacatttgac tcgcggggga aacgtttacg gagtcttcat actggcactt ttttatgctg
      241 ctgactactc ttcgtcgtcg ccatcgacat gcgcacgaat cagcgccata aacggtttac
      301 caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga
      361 tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg tacggcggga
      421 cattactttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat
      481 catagttgcc gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca
      541 cggcaagttg caaagaggat tcgccgcgca gcaccgggcg cgcggcctct gtcagttgta
      601 gggcagaatg ctgggcaata ttttgcgtca ccaggccgag atgaatcagc tggcggatca
      661 cgctcaccca atgttcatgg cttttatcac ggcccatacc atagactttc agtttgtcat
      721 gaccatagtc gcggatacgc tggttattag caccacgaat cacttccacc acataaccca
      781 tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgagca tcggttgaac
      841 cgtcgtactg tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc
      901 cttcgccaaa atagttcagc aataccagac gacggcaagt ttgcgcttcg gcaaacgcgc
      961 ccatcgcatt gagtttgtgg cgctcgatat cctgcaactg cccctgcggc ttctcttcca
     1021 gacaacggcg cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcaggca
     1081 ggccatcacg cccggcgcga ccggtttcct gataatagga ttcgatattg cgcggaatat
     1141 caaagtggac cacgaagcga acgtttggtt tattgatgcc catgccgaac gccaccgtcg
     1201 ccaccacaat ttgcagatca tcgcgctgga atttctcctg cacgtcggcg cggacattat
     1261 tttccagccc ggcatgatag gccgccgcgc taataccctt gctttgcagg cgcgcagcgg
     1321 tgtcttctac tttcgcccgg ctgttgcagt agataatgcc agacttaccg cgctgttcct
     1381 gcacgtagcg catcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat
     1441 tcggacggtc aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga
     1501 tatcctggcg cgtggtgtcg tcggctgtgg cggtcagcgc cataaacggc agcgtcggga
     1561 accgctggcg caactgaccg agcgcggcat attccgggcg gaaatcgtgg ccccattggg
     1621 agatacagtg cgcttcatcg acggctaata acaccggatt ccagtgcgcc agatgctcaa
     1681 gaaagttatc cagcatcagg cgttccgggg cgatatacag taagcgaatt tgcccggtgc
     1741 ggcagcctgt catcacttca agttgctgtt cgcgggtttg cgtcgagtta aggcacgccg
     1801 ccgccacgcc gttggcctgt agttgatcca cctgatcttt catcaacgaa atcagcggtg
     1861 aaacaaccac ggtaaggccg tttagcaata aggcaggaat ttgatagcaa agggattttc
     1921 cgccaccagt gggcataaca accaggcaat cgcgaccgga aagcacagtg tcgataattt
     1981 cttcctggcc ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc tgtttagctc
     2041 cggactccag attcaacact tccgcctgcg ccacattcac cccgattgcc tgaaataaaa
     2101 acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct ctgagagggt
     2161 aatcctggat gctggattcc agatttgcca accactcaac cgttcatcaa aacaagtcgt
     2221 ttagcataac ccctacaccg acacgggtct ggttgaagtt atagtcgatg agcgattctc
     2281 catagccgct gtaaacctga gtataaaggc gcacatgttt ggtgatcggg taacttaagc
     2341 ctaactccgc gccgccgtag ccggtgttcc agttgtactg tcctttcgca ctgagcaccg
     2401 catcaccgag gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatccg
     2461 ggttatcgtc agtattaccc accacatacc acggcttcac ttctaccagc cagttaccgt
     2521 tttctgccat caggcgagta taaaggcggt tccagctgcg ggaggtcggg tctgaacgcc
     2581 cgttagagtc gtggttatac cccatctcca catcgcgcag cgtccaacct gcaaaacggt
     2641 aatcggtggc aaaaccgagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag
     2701 actcttcgct attggacagt tgccaccagg atttttgcgt ataagacgca cccaacaccg
     2761 agtttgggcc taaaatccca cgccacagcg gaaatgccag gctcaactga aactttactt
     2821 catccttacg cgcattttcc gcccagtcgt aactggcaat cgcttcttta ttcagatcgc
     2881 tggtttgggt gtagatgagg tagttggtgt cataaggata gagcgtgaac ggattgtcat
     2941 gctcctgcag catattggcg ataatactgc cacgcaccgc tggcgcgtca tgcacctctt
     3001 tcaccgttgc ctcttgtgca tatactgcca taggcaacat aaacaccggc aacaaccagc
     3061 cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt attgataagg tgaatgtttt
     3121 tctgcgggct attttacata cttaactcca ttgccgttag ttatcccttc ctaaagtaga
     3181 aaccaacaac aaagaagcat aaaatcaaca tttcattaac caatgaagtg taaggatgat
     3241 atgtctgccg tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat
     3301 cacatgccat ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca
     3361 cagctggcct ttaaaaatca gccgatgatg gtgggtaact gggcgcaaag cattttgcac
     3421 ggcggagtca ttgcgtcggc gctggatgtc gccgccggtc tggtgtgcgt gggaagtacc
     3481 ttaacccgcc acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg
     3541 accattgatc ttcgcgttga ttatctgcgc ccaggcagag gcgagcgttt tactgctacc
     3601 agtagcctgt tgcgtgcagg caataaagtc gccgtcgccc gcgttgaatt acacaatgaa
     3661 gaacagcttt atattgccag tgccaccgcc acctatatgg taggttgaag acataaaagc
     3721 aggtaaactg ctgttaccaa ttaaaaatga gtattcccga tggatgcaaa acaaacgcgg
     3781 cagggcgtat tactcgctct tgccgcttat tttatttggg gtattgcgcc agcatacttc
     3841 aagctgattt actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt
     3901 ttctttatgg tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt
     3961 cagacgccac agaaaatttt tatgctggca gtctctgccg tgctgattgg tggcaactgg
     4021 ctactgttta tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt
     4081 attaacccgc tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg
     4141 atgcaatggc tggcggtgat tctggcgata tgtggcgtgt tggtccagct gtggactttt
     4201 ggttcgctac ctattatcgc gctggggctg gcatttagtt ttgccttcta cggcctggtg
     4261 cgcaagaaga ttgcggtaga agcacaaacc ggcatgttaa ttgaaaccat gtggctgctg
     4321 cccgtggcgg cgatttacct gtttgctatt gccgacagtt caaccagcca tatggggcaa
     4381 aacccgatgt cgctgaattt actgctgatc gccgccggta ttgtcactac cgtaccgctg
     4441 ttgtgtttta ctgccgctgc cacgcgcctg cgtctctcaa cgttaggctt ttttcagtac
     4501 attggcccga cgctgatgtt cctgctggct gtgacgtttt atggtgaaaa accgggtgcc
     4561 gataagatgg tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggacgcg
     4621 atttatacgc agcgtagaac gtccaaatca aaaataaata tattctgaaa aaatatgccg
     4681 actcttgtat gagagtgggc atattgccat gctgacagga gactcacaca aggatgtaaa
     4741 atttatgtta cgtatgttta tcccaacatc taacggtaag atttccagac gccgatatat
     4801 attttcattt attttaataa atttcatttt tgcctttctt attatctttt ttaacgatgg
     4861 ggaagcaggt tttcttgtta tagtctctac catcgcactc cattatctcg tcatcaacat
     4921 gaattgccaa cgactccgtg atagtggttt catatatata aaaatttatg tttttggtac
     4981 atta
//
LOCUS       DAJTRQ010000200         4912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000200 DAJTRQ010000000
VERSION     DAJTRQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..609
                     /locus_tag="OO332_003944"
                     /pseudo
     CDS             1..609
                     /locus_tag="OO332_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            673..1599
                     /gene="glsB"
                     /locus_tag="OO332_003945"
     CDS             673..1599
                     /gene="glsB"
                     /locus_tag="OO332_003945"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5944204.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1566..1958
                     /locus_tag="OO332_003946"
     CDS             1566..1958
                     /locus_tag="OO332_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5944205.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2097..3515
                     /locus_tag="OO332_003947"
     CDS             2097..3515
                     /locus_tag="OO332_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5944206.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3742..>4912
                     /gene="uxaB"
                     /locus_tag="OO332_003948"
     CDS             3742..>4912
                     /gene="uxaB"
                     /locus_tag="OO332_003948"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5944207.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLT"
BASE COUNT         1128 a         1147 c         1325 g         1312 t
ORIGIN      
        1 ttcccatcat tcttgcaggt aacggctact tacttaaaca tgcgccgaat gtgatgggct
       61 gtgctcagct cattgcccag gtgtttaaag atgcgggaat cccgcaaggc gtatatggct
      121 ggctgaatgc cgacaacgac ggtgtcagtc agatgattaa agactcgcgc attgctgctg
      181 tcacggtgac cggaagtgtt cgtgctggag cggctattgg cgcacaggct ggagcggcac
      241 tgaaaaaatg cgtactacta tccgccaacg gttctggcga atgttacccc agaaatgacc
      301 gcgtttcggg aagaaatgtt tggccccgtt gctgcaatca ccattgcgaa agatgcagaa
      361 catgcgctgg aactggctaa tgatagtgag ttcggccttt cagcgaccat ttttaccacc
      421 gacgaaacac aggccagaca gatggcggca cgtctggaat gcggtggggt gtttatcaat
      481 ggttattgtg ccagcgacgc gtgagtggcc tttggtggcg tgaaaaagag tggctttggt
      541 cgtgagcttt cccatttcgg cttacacgaa ttctgtaata tccagacagt gtggaaagac
      601 cggatctgac gcgacatatt cagctctgat atactcgcag gtcttttcag acctgcggtc
      661 caggagtaga aagtggcagt cgccatggat aatgcaattt tagaaaacat cttgcggcaa
      721 gtgcggccgc tcattggtca gggtaaagtc gcggattata ttccggcgct ggctacagta
      781 gacggttccc gattggggat tgctatctgt accgttgacg gacagctttt tcaggccgga
      841 gacgcgcaag aacgtttttc cattcagtct atttccaaag tgctgagtct cgttgtcgcc
      901 atgcgtcatt actccgaaga ggaaatctgg caacgcgtcg gcaaagatcc gtctggatca
      961 ccgttcaatt ctttagtgca actggaaatg gaacagggta ttccgcgtaa tccgttcatt
     1021 aatgccggtg cgctggtggt ctgcgatatg ttgcaagggc gactaagcgc accacggcaa
     1081 cgtatgctgg aagtcgtgcg cggcttaagc ggtgtgtctg atatttcata cgatacggtg
     1141 gtagcgcgtt ccgaatttga acattccgcg cgaaatgcgg ctatcgcttg gctgatgaag
     1201 tcgtttggca atttccatca tgacgtgaca accgttctgc aaaactactt tcattactgc
     1261 gctctgaaaa tgagctgtgt agagctggcc cggacgtttg tctttctggc taatcagggg
     1321 aaagctattc atattgatga acctgtggtg acgccaatgc aggcgcggca aattaacgcg
     1381 ctgatggcga ccagtggtat gtaccagaac gcgggggagt ttgcctggcg ggtgggacta
     1441 ccggcgaaat ctggcgttgg tggcggtatt gtggcgattg ttccgcatga aatggccatc
     1501 gcagtctgga gtccggaact ggatgatgca ggtaactcgc ttgcgggtat cgccgtcctt
     1561 gaacaattga cgaaacagtt agggcgttcg gtttattaag gcagtctctc gatccactct
     1621 tcgcgcgttt atcccgttca aaatttcgct ctcgctttcg tctgggcatg aaagagcgtc
     1681 agtattgcct ggagaaaggc gcgccagtca tcgaacaaca tgcggcggat tttgtcgcta
     1741 aacgtcttgc tccggcatta ccggctaatg acggtaagca accccccatg cgcgggcatc
     1801 cggtgtttat cgctcagcac gccaccgcga cctgctgtcg cggttgtctg gctaagtggc
     1861 ataacattcc gcagggtgtt tcgttaagcg aagaacaaca acgttatatc gtggcggtaa
     1921 tctatcactg gctggttgta cagatgaatc agccatgaga attgcggaaa acgataaaat
     1981 agcattccca gggtaccttt atttgccgcc gctgttagcg aacatgctat cctttatgag
     2041 aatttactta tcacgttgag ataattgcca cccgcaattg tatggacaga taattaatgc
     2101 atgtacaccc catttcaact ttccgtttgt tccaggaagg tcatctgcta cgtaatagca
     2161 tcgctatttt tgcgctaacc acgctgttct attttattgg tgcagagtta cggctggttc
     2221 acgaactttc tcttttctgg ccgctgaatg gcgtaatggc gggggtgttt gcccgctatg
     2281 tctggcttaa tcgactgcat tactatgcga tcagttatgt ggcgatgctg gtttatgatg
     2341 ccataaccac cgaatgggga ctggtttcac tggttatcaa tttctccaat atgatgttta
     2401 ttgttaccgt cgccttactg gtcgtgcggg ataagcgtct ggggaaaaat aagtatgagc
     2461 cagtaagtgc cttacggcta tttaattact gtctgattgc cgcattatta tgcgctattg
     2521 tcggggcgat tggttcggtc agtattgata gtctggattt ctggcctttg cttgcggact
     2581 ggttcagtga gcaattctca acgggcgtgt tgatcgtgcc ttgtatgctg acgttggcta
     2641 ttcctggagt gctgccgcgc tttaaagcag aacagatgat gcccgccatc gcgcttattg
     2701 tgtcggttat tgcctcggta gtcattggcg gagcggggag tctggcgttc ccgctgcctg
     2761 cattaatctg gtgtgcagtg cgctatacgc cgcaggtaac atgtctgttg acctttgtca
     2821 ccggtgcggt ggaaatcgta ctggtggcaa attcggtgat tgatatctcg gtcggttcgc
     2881 cgttctccat tccggaaatg ttctccgcac gtctcggtat tgccacgatg gcgatatgcc
     2941 caattatggt ttcttttagc gtggcagcga tcaatttgct aatgaagcaa gttgcgctgc
     3001 gagccgactt tgattttctg actcaggttt actcacggtc cggtctttat gaggcgctga
     3061 aaagtccatc gctgaaacag acacaacatc tgacggtcat gctgcttgat atcgactatt
     3121 tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta agcgtgtttg
     3181 cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg ggcggcgaag
     3241 aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg gcggaaaaaa
     3301 tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt tatctcacgg
     3361 taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat gattttaata
     3421 aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg cgcaaccgta
     3481 ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc cgcgcgattt
     3541 gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct cgattaaacc
     3601 atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag ttgatcaaaa
     3661 caggggttcg gcgtattaaa gtttgctaac ctgtcgcacc aattcaaaca ggttgtatga
     3721 ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc cggtgcacag
     3781 tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt tgttgactgg
     3841 caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt tgttcgtccg
     3901 attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtacac caccattatc
     3961 cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg ctcggttaat
     4021 cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca caacccggaa
     4081 atgcgctttg ttttctctaa caccaccgaa gcgggtatca gctaccatgc gggcgataaa
     4141 ttcgatgacg cgccagcggt aagctatccg gcaaaactga cccgtctgct gttcgaacgc
     4201 tttagccatt tcaacggtgc gctggataaa ggttggatca tcattccgtg tgagttgatt
     4261 gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga gtgggcactg
     4321 ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac gctggtggac
     4381 cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga actgggttat
     4441 cacgatggtt tcctcgacac cgctgaacat ttttacctgt ttgtgattca ggggccgaaa
     4501 tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat tgttgacgat
     4561 attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac cgcgctggtg
     4621 ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga tgctgaaatt
     4681 tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga tttgcctcgt
     4741 gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc gtacattaag
     4801 catcagctgc tgtctatcgc gctcaacggt atgaccaagt tccgcacccg catcctgccg
     4861 cagctgctgg cagggcagaa ggcaaacggc acacttccgg cgcgccttac tt
//
LOCUS       DAJTRQ010000201         4773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000201 DAJTRQ010000000
VERSION     DAJTRQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            61..1176
                     /gene="adrA"
                     /locus_tag="OO332_003949"
                     /gene_synonym="dgcC"
     CDS             61..1176
                     /gene="adrA"
                     /locus_tag="OO332_003949"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5944208.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1193..2002)
                     /gene="proC"
                     /locus_tag="OO332_003950"
     CDS             complement(1193..2002)
                     /gene="proC"
                     /locus_tag="OO332_003950"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5944209.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2122..2580
                     /locus_tag="OO332_003951"
     CDS             2122..2580
                     /locus_tag="OO332_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5944210.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2763..3287
                     /gene="aroL"
                     /locus_tag="OO332_003952"
     CDS             2763..3287
                     /gene="aroL"
                     /locus_tag="OO332_003952"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5944211.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3337..3528
                     /gene="yaiA"
                     /locus_tag="OO332_003953"
     CDS             3337..3528
                     /gene="yaiA"
                     /locus_tag="OO332_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5944212.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3786..4463
                     /locus_tag="OO332_003954"
     CDS             3786..4463
                     /locus_tag="OO332_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5944213.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4535..>4773
                     /gene="ppnP"
                     /locus_tag="OO332_003955"
     CDS             4535..>4773
                     /gene="ppnP"
                     /locus_tag="OO332_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5944214.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEF"
BASE COUNT         1157 a         1134 c         1281 g         1201 t
ORIGIN      
        1 ccgaaatttc atacttctgc ctttagcccc gtctctataa tttgggaaaa ttgtttctga
       61 gtgttcccaa aaataatgaa tgatgaaaac tttttcaaaa aagcggcggc gcatggggag
      121 gaacctcctt taactcctca aaacgaacat cagcggtccg ggctgcgctt cgcccgtcgc
      181 gtcagactac cccgtgcggt tggcctggct ggcatgttct taccgattgc ttcaacgctg
      241 gtttcgcacc cgccgccggg ctggtggtgg ctggtgttgg tcggctgggc gttcgtctgg
      301 ccgcatttag cctggcagat agcgagcagg gccgtcgatc cgcttagccg ggaaatttac
      361 aacttaaaaa ccgatgcagt attagcggga atgtgggtag gcgtaatggg cgtaaacgtg
      421 ctgccttcca ccgctatgtt gatgattctg tgtctgaatt tgatgggggc aggcggcccc
      481 cgtctgtttg tcgcgggtct ggtgttgatg gtggtttcct gccttgtcac cctcgagctt
      541 acgggcatta ccgtgtcgtt caatagtgcg ccgctggaat ggtggctctc ccttcccatt
      601 attgtcgttt atcctctgct gtttggctgg gtcagctacc agacggcaac caaactggcg
      661 gaacataaac gcaggttgca ggtcatgagt actcgcgacg gcatgacggg cgtgtataac
      721 cgacgtcatt gggaaactat gttacgcaat gaatttgata actgtcggcg gcataatcgc
      781 gatgcaacgt tactgattat cgatatcgac catttcaaga gcatcaacga tacctggggt
      841 catgatgtgg gcgatgaagc gattgtggcg cttacccgac agttacaaat taccctgcgc
      901 ggtagcgatg tgattggtcg gtttggcggc gatgagtttg cagtaatcat gtccggtacg
      961 ccagctgaga gcgccattac cgccatgtta cgggtgcgtg aagggctaaa cacgttacgc
     1021 ttgccgaata cgccacaggt aactttacgg attagtgtgg gggttgcgcc gctgaaccca
     1081 caaatgagtc actatcgtga gtggttgaaa tcggcagatt tggcgcttta caaagcaaag
     1141 aaagccggac gtaaccgcac cgaagtggcg gcctgacgtc cggcgaaagt catcaggatt
     1201 tgctgagttt ttctgatttt tccatacact tcgtcatcgc ttcgatcact gcggcacgga
     1261 agcctttctc ttccagtacg cgtaccgctt caatggtggt acctcccggt gagcagacca
     1321 tatctttcag tgcccccgga tgttctcccg tttccagcac catttttgcg gaacccatta
     1381 ccgcctgagc ggcaaattta tacgcctgga cgcgtggcat cccgcccagc acggcggcgt
     1441 cggccatcgc ttcgataaac ataaatacgt aggctggcga agaaccgctc acaccgacca
     1501 ccgggtggat catcggctca gcaattactt ccgcttcgcc aaagcagcgg aaaatattca
     1561 gcacatcagc ggtatcttct ggggttacca gcgcgtttgg cgttacggag gtcatcccgg
     1621 cattaaccag cgcgggagtg ttcggcatgg cgcggataat tttccggtca tggcccagcg
     1681 cgcgggcaag ctggtcgagc gtgacacctg cagcaataga aacgaccaga gagtctttat
     1741 tcaggctgga ggtgatttcg ctaagcactt taatcatgat gccaggttta acggcagcaa
     1801 aaatgatgtc ggcgatttgc gccacttctt gcgccgattc tgcggcgttg atgccgaact
     1861 ggtcatgcag ggcggcgact ttatccgggg agggggtgta tacccagatt tgccctggaa
     1921 gcacctgacc gctggcaatt agaccgccga gaatggcttt tcccatattg ccgcagccaa
     1981 taaaaccgat tttcttttcc attgcctcac tcctgccgtg aaattcattg ttttgataat
     2041 cgctggcaga agcataaaca gaactatgcc ggaaggcaaa agcgcgacac aatagaggat
     2101 tacccaacaa aggatgactt tatgacaatt tgggtggatg ccgacgcgtg tcccaatgta
     2161 attaaagaga ttttgtatcg cgcggcggaa cgtatgcaga tgccgctggt actggtagca
     2221 aaccagagtt tacgcgtgcc gccatcgcga tttattcgta cgctgcgcgt cgcggcaggt
     2281 ttcgacgttg ccgataacga aattgtccgg cagtgtgaag cgggcgattt ggtgatcacc
     2341 gcagatatac ctttggctgc tgaagccatc gagaaaggcg cagcggcgct taatccgcgc
     2401 ggcgaacgtt acacgccagc gaccattcgt gagcgcctga cgatgcgcga ttttatggat
     2461 accttacgtg ccagtgggat ccagaccggc ggaccagata gcctttcaca acgtgaccgc
     2521 caggcctttg ccgcggagct ggagaagtgg tggctggaag tgcaacgtag tcgtggctaa
     2581 atgtaattta ttatttacac ttcattcttg aatatttatt ggtatagtaa gggatgtatt
     2641 gagattttca ctttaagtgg aattttttct ttacaatcga aattgtacta gtttgatggt
     2701 atgatcgcta ttctcatgac accggctttc gccgcattgc gacctattgg ggaaaaccca
     2761 cgatgacaca acctcttttt ctgatcgggc ctcggggctg tggtaaaaca acggtcggaa
     2821 tggcccttgc cgattcgctt aaccgtcggt ttgtcgatac cgatcagtgg ttgcaatcac
     2881 agctcaatat gacggtcgcg gagatcgtcg aaagggaaga gtgggcggga tttcgcgcca
     2941 gagaaacggc ggcgctggaa gcggtaactg cgccatccac cgttatcgct acaggcggcg
     3001 gcattattct gacggaattt aatcgtcact tcatgcaaaa taacgggatc gtggtttatt
     3061 tgtgtgcgcc agtatcagtc ctggttaacc gactgcaagc tgcaccggaa gaagatttac
     3121 ggccaacctt aacgggaaaa ccgctgagcg aagaagttca ggaagtgctg gaagaacgcg
     3181 atgcgctata tcgcgaagtt gcgcatatta tcatcgacgc aacaaacgaa cccaaccagg
     3241 tgatttctga aattcgtagc gccctggcac agacgatcaa ttgttgattt tagagcgcct
     3301 atacttaacg ttcatcccgt gaaataagga agaacgatgc caacgaaacc gccttatcct
     3361 cgtgaagcat atatagtgac gattgaaaaa ggaaagccag gacagacggt aacctggtac
     3421 caactcagag ccgatcatcc taaaccagac tcgttgatca gtgaacatcc gacggctcag
     3481 gaagcgatgg atgcgaaaaa acgctatgag gaccctgaca aagagtgacc gcatcagact
     3541 gctcggaagg gattctgagt gccactacaa gggatctgcg tcacattttt cataattcat
     3601 gtttttctaa taattagaat attaaacaat aacattccat tactggaatc atttggaatc
     3661 tttacattat gccgtgcacg tctgctgcta cgctttttgt catttgtagc acaagtaagt
     3721 gtcagcagtg gtgcttcaca cttgcccggt aattaacgac gaaagaaaag taaggtggat
     3781 gaacaatgag tgcgtcgttg gcgatcctga ccatcggcat tgtacctatg caggaggttt
     3841 tgccgctcct gacggaatac attgacgaag ataatatttc ccatcatagc ctgctgggga
     3901 agttaagtcg tgaagaagtg atggcggagt acgcgccaga agcaggcgaa gacaccattc
     3961 tcacattatt aaatgacaac cagctggccc atgtttcgcg tcgtaaagtg gagcgagatc
     4021 tgcaaggtgt ggttgaagtg ctcgataatc agggttatga cgtcatttta ttaatgagta
     4081 cagcaaacat tagtagtatg actgcgcgta atacgatctt tcttgagccg tcgcgaatat
     4141 tgcctccact ggtttcctct attgttgaag atcatcaggt gggggttatc gttccggttg
     4201 aggagatgct gcccgttcag gcgcaaaaat ggcaaatttt gcagaaatcg ccggtatttt
     4261 cattgggtaa ccccattcat gattcagaac aaaaaatcat tgatgctggg aaagaattac
     4321 tggcaaaagg ggctgatgtc atcatgctgg attgtttagg attccaccaa cgtcatcgcg
     4381 atttactgca aaaacagctc gatgttcctg tcttgctctc taacgtattg attgcacggc
     4441 tggctgcgga attactggtg taattttgcg tgacagccag cgcctctggc ccctatagtg
     4501 aagtagatgt tcaactacca gacagggcca gtttatgctt caaagtaatg agtacttttc
     4561 cggcaaagtg aaatcgatcg gcttttccag cagcagcact ggtcgcgcca gcgtgggtgt
     4621 tatggttgaa ggcgaataca ccttcagcac cgctgagccg gaagagatga cggtaatcag
     4681 tggcgcgctg aatgtgttac tgcctgacgc gaccgactgg caagtgtatg aagccggttc
     4741 ggtgtttaat gttcccggtc acagtgagtt tca
//
LOCUS       DAJTRQ010000202         4754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000202 DAJTRQ010000000
VERSION     DAJTRQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            278..808
                     /locus_tag="OO332_003956"
     CDS             278..808
                     /locus_tag="OO332_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5944215.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            815..1048
                     /locus_tag="OO332_003957"
     CDS             815..1048
                     /locus_tag="OO332_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5944216.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1628..2746)
                     /locus_tag="OO332_003958"
     CDS             complement(1628..2746)
                     /locus_tag="OO332_003958"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5944217.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2864..2953)
                     /locus_tag="OO332_003959"
     CDS             complement(2864..2953)
                     /locus_tag="OO332_003959"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944218.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3389..3550)
                     /locus_tag="OO332_003960"
     CDS             complement(3389..3550)
                     /locus_tag="OO332_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR5944219.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4202..4393)
                     /locus_tag="OO332_003961"
     CDS             complement(4202..4393)
                     /locus_tag="OO332_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5944220.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4457..4687
                     /locus_tag="OO332_003962"
     CDS             4457..4687
                     /locus_tag="OO332_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR5944221.1"
                     /translation="MLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
BASE COUNT         1328 a          984 c         1199 g         1243 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
      181 acagaggcag ggattgacgt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
      241 aacggcggga agaatgtcag actgaaagga gcgttttatg agcaatcttt tacagacggg
      301 tgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
      361 cgagttcagg aagctcgaag aatctgtaaa cagggaactg atctcaaacg aatctttaat
      421 caggaacgct atcaacgacc atacatcagc cctgaaagaa cttctggaga ggtatcagaa
      481 aacgaccgtc gatacgatgg acgcacactg gaaaaccgta ctgaaaatga gcgtgaaacg
      541 ctggctgtgg ctgattatcg tctcagtact gatgtttgcc acgaccggga gcctgctgtg
      601 gtgccagggc atgaagataa acgcgaacat gaatattctc cgcgagcaga aagagagtct
      661 ggagaagctg aacgcgaaga catggggcgt gcgttatcac gaggacagca acggacgttt
      721 tctggtgttg cccgagggga tgaaagccga aacgaactgg acgatggaaa acgggaaaca
      781 gaatgccgta aggctggtac aggagtaaca gagcatgacg gagctggaaa gacatttgct
      841 gaacgcatta gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc
      901 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
      961 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     1021 ccagttatac agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat
     1081 gcgttatccc tggagcgtga gcgacagccg gaaatacagg aacggacact ggatggccct
     1141 tcgctgggat ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt
     1201 ttgggataat ggttggtttg gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     1261 acataggaac gcgttaaaac gcatacgggc gcgattctgg tgtgtctttt gttgctgtgt
     1321 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctcaggtcc
     1381 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagctgtctc tggctggcaa
     1441 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     1501 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     1561 cgaacgcgtt tcagcggtgc gtacaatgag atgttatggt aaattcaagt cttagatttt
     1621 atgatgttta attagctttt tttgcgttta taatgttcat cttttttatt aaaattgatt
     1681 caatctttga tgtgggtatt aatattttat ttgacaaata gaaagttaaa aaaaacacca
     1741 ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt attgatgtta
     1801 atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta gcatatgagt
     1861 tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa atgataatgc
     1921 ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt ttgaactcat
     1981 taggccctat taataatact gtcaatatac atataacaac cgatagaata actgtggtgt
     2041 ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac tcaggaagtc
     2101 ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt gatatcggga
     2161 atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta aaggtattca
     2221 taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca ataaaccact
     2281 cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca tcaagggcaa
     2341 aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat acgagaggga
     2401 ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa gagataaaaa
     2461 agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa taatcaatgg
     2521 tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa aacaagctca
     2581 cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc ctggtttggc
     2641 taaatatgta gccataatga gatataataa caaaaatgca ggctgttact cgcaatgtat
     2701 caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta tcgcctttag
     2761 ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc gaaaggatga
     2821 ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg gtgctgatag
     2881 ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct tttcttgtta
     2941 gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac cgaacggttt
     3001 cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga aaaacgccct
     3061 caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt ccattagtcg
     3121 attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc atcagacccc
     3181 ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac aacagatcta
     3241 cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa ccgccctgca
     3301 aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta actggcttat
     3361 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
     3421 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3481 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc acatgttctt
     3841 tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg agtagatacc
     3901 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3961 ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt atggcgcact
     4021 tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg ctatagctac
     4081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatat
     4201 gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg
     4261 cgtggtcgtg aagcgattca cagatatcag cctgttcatc cgcgtccagt tcgttgagct
     4321 tctccagcag cgttaatgtc tgccttctga taaagcgggc catgttaagg gcggtttttt
     4381 cctgtttagt cactgatacc tccgtgtaag ggggatttct gttcatgagg taatgatacc
     4441 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg tccggttact
     4501 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
     4561 ggttgcccgt tccgggaaac tgccgacact ggcaccgccg ttactgcgtc agctggctgc
     4621 catcggggac gagctgcgcc ggctgcgtct ggccgtcagg gaacagggga cgcgggatga
     4681 tagttaagtt tcatgcccgg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg
     4741 gaagggagcg taac
//
LOCUS       DAJTRQ010000203         4684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000203 DAJTRQ010000000
VERSION     DAJTRQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            103..1011
                     /locus_tag="OO332_003963"
     CDS             103..1011
                     /locus_tag="OO332_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5944222.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFY
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1057..3048)
                     /gene="tkt"
                     /locus_tag="OO332_003964"
     CDS             complement(1057..3048)
                     /gene="tkt"
                     /locus_tag="OO332_003964"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5944223.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3326..4084
                     /gene="loiP"
                     /locus_tag="OO332_003965"
                     /gene_synonym="yggG"
     CDS             3326..4084
                     /gene="loiP"
                     /locus_tag="OO332_003965"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5944224.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4290..>4684)
                     /locus_tag="OO332_003966"
     CDS             complement(4290..>4684)
                     /locus_tag="OO332_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HCR5944225.1"
                     /translation="DPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIV
                     GDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPA
                     YDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1115 a         1251 c         1245 g         1073 t
ORIGIN      
        1 tttttcgcta taccccttta tagattctga attctcacct atcggctgtt ttctcctcat
       61 ctcatgtgac atattcccat actttactca aggaagattg tcatgaaagc acctgtttca
      121 ttaacctcat ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct tgccgtggtt
      181 gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc
      241 tacggaaaaa tcgtaaaagt tgccgagccg gggctgggtt ttaagatccc ttttatggaa
      301 agcgtggaga aaatttccac gcgtaatcag gctgtggtct atcagggatt acaggcctac
      361 agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttctacat taaaccttca
      421 gaagcaggcg cggtttatac cacctacaac actattgaag cattaaaaga tcgtctaatt
      481 gtccgtcagt tgccaactca gctggagaac attttcggcc aatataccgc catcagcgcc
      541 gtacaggatc gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa agccgttgtt
      601 gggccagtgg taattgacgg ggtccagatt gaaaatattg atttttctga cgcttatgaa
      661 aagtcgattg aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa gcaaaatctg
      721 gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga agcagacagt
      781 aaactggcgg cagacaaagc agaagcggaa actattcgcg taagaggcgc ggcagaggct
      841 gaaactattc gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg cggtgaagct
      901 ctgcgggata atcccggact tgtagccctc accactgccg aacgttggga cggtaaactg
      961 cccgaaacca tgatccccgg cagcacagtt ccgtttatct ctactaagta aaaaaagggc
     1021 cgctttcgcg gcccttctca caaaacgtct tactgattac agcagcgctt ttgctttcgc
     1081 aacaacgtta tcaacagtga agccgaactc ttcaaacagc agctctgccg gagcagattc
     1141 accgaaggtg gtcatgccga cgatagcgcc gttcaggcca acatacttgt accagtagtc
     1201 agcaataccc gcttctaccg caacgcgtgc agtaaccgct ttcggcagta cggattcacg
     1261 gtaagccgca tcctgcttgt caaatgcgtc ggtagacggc atggacacca cgcgcgcttt
     1321 cacgccttcg gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt cagaaccggt
     1381 ggcgatgaag atcagttccg gctgaccagc acagtctttc agcacataac caccgcgcgc
     1441 gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc aggttctgac gggagaggat
     1501 aagcgcagtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg cggattcaac
     1561 ctggtcacac ggacgccatg tagacatgtt cggggtcacg cgcagagaag cgacctgctc
     1621 taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg tgtaaaccat
     1681 cacctggcgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt attccacgaa
     1741 catcaggaag gtggaggtgt acggcaggaa gccaccgtgc agggagatac cgttagcaat
     1801 cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacctgcag catcttcgtt
     1861 gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag cagagccgcc
     1921 gaggaattca ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg ctttacggct
     1981 ggcgattttc gccggattag cctgcagttt agcgataaac tctttcgctt tggcgtcgaa
     2041 gtcagacggc atttcgcctt tcatacggcg ggtaaattca gccgcttcct gcggataagc
     2101 tttcgcgtaa gccgcaaact tctcattcca tgcagattct ttcgcctggc ctgcttcttt
     2161 cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt tccagcccag
     2221 ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg agtcgtgagt
     2281 accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca gggacggttt
     2341 gtcagtcact gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt catgaccgtc
     2401 gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt cgtcggtgaa
     2461 ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg caatcagttt
     2521 acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt ccatcatgca
     2581 gccgtcgccc atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg gacggttaaa
     2641 ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa taccctgacc
     2701 cagcggaccg gtggtggttt ccacaccagc ggtataacct acttccgggt ggcccggagt
     2761 tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt aaccggtgag
     2821 gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga agcggtcacg
     2881 gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca ggacttcggc
     2941 aatgtcagcc atacccatag gggcacccgg gtgaccggat ttggctttct gtactgcgtc
     3001 catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt tgactccaga
     3061 tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg gctacgccgg
     3121 aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat ccttttgccg
     3181 cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg tccgctgcgc
     3241 ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt ttagaataat
     3301 cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag caatgagcgt
     3361 ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct catcaggagc
     3421 ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga gcgatcaggc
     3481 atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat acgctaaacg
     3541 tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg tgaattacaa
     3601 agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta tccgcgtcta
     3661 tagcgggctg atggatatga tgacagataa cgaagtcgaa gcggtgatcg gtcacgagat
     3721 ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg gtacaaatgc
     3781 cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc aatcacaact
     3841 tggtgatctg ggcgagaaat tagtcaattc gcaattctcc cagcgccagg aagcagaagc
     3901 cgatgattat tcttacgatc ttctgcacca acgcggcatc agcccggcag gtcttgccac
     3961 cagctttgaa aaactggcga agctggaaga aggccgtcaa agctcaatgt ttgacgacca
     4021 tcctgcatcc gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg atgggattaa
     4081 gtaaagcctg gtggtgtcga acgtaggtca gataaggcgt tcacgccgca tctgacatta
     4141 atggcacatt tgttaccttg tgcgcattgc cggatgcgat gctggcgcat cttatccggc
     4201 ctacgggtgc cgaacgtagg tcggataagg cgttcacgcc gcatccgaca ttaatggcac
     4261 gttttacccg tgcgcatcgc atctggtgct tactcgccct ttttcgccgc ctgaatatac
     4321 agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc
     4381 ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc gcgtaccagt
     4441 ttaatggcgc gatcggaggt caggccgcca atcactggcg taccggttcc cggcgcgaaa
     4501 gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc cacaatctgt
     4561 ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg
     4621 gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga atgattcggg
     4681 tcga
//
LOCUS       DAJTRQ010000204         4655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000204 DAJTRQ010000000
VERSION     DAJTRQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..213
                     /locus_tag="OO332_003967"
     CDS             <1..213
                     /locus_tag="OO332_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5944226.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="OO332_003968"
                     /pseudo
     CDS             356..577
                     /locus_tag="OO332_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..660)
                     /locus_tag="OO332_003969"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="OO332_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="OO332_003970"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="OO332_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5944227.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="OO332_003971"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="OO332_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="OO332_003972"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="OO332_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5944228.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1624 a          833 c          866 g         1332 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa gtgga
//
LOCUS       DAJTRQ010000205         4608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000205 DAJTRQ010000000
VERSION     DAJTRQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.208"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..76
                     /locus_tag="OO332_003973"
     tRNA            1..76
                     /locus_tag="OO332_003973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Thr,seq:ggt)
     gene            116..231
                     /gene="rrf"
                     /locus_tag="OO332_003974"
     rRNA            116..231
                     /gene="rrf"
                     /locus_tag="OO332_003974"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(294..1052)
                     /locus_tag="OO332_003975"
     CDS             complement(294..1052)
                     /locus_tag="OO332_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5944229.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1060..2163)
                     /locus_tag="OO332_003976"
     CDS             complement(1060..2163)
                     /locus_tag="OO332_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5944230.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2173..3343)
                     /locus_tag="OO332_003977"
                     /pseudo
     CDS             complement(2173..3343)
                     /locus_tag="OO332_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3411..4436)
                     /locus_tag="OO332_003978"
     CDS             complement(3411..4436)
                     /locus_tag="OO332_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5944231.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1266 a         1202 c         1095 g         1045 t
ORIGIN      
        1 gctgatatgg ctcagttggt agagcgcacc cttggtaagg gtgaggtccc cagttcgact
       61 ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga atttgtctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccaga catcaaatca
      241 agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt caattaatgg
      301 attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa aaattcatca
      361 ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc agcgacggta
      421 cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg cgccagccca
      481 atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt tggttcatca
      541 aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg ttgctgctga
      601 ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac ccgctcaagg
      661 taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca aatcggtgcc
      721 agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg aaataccatt
      781 cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc gatgccatct
      841 acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat ggttgtcgat
      901 ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac ggttaaattt
      961 atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag cgtaatcatc
     1021 gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg tacgcccggt
     1081 gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa agatccagta
     1141 gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg gatcaacggt
     1201 tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta ggctggtatc
     1261 tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca acgcctgtgg
     1321 cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg ccgcttcata
     1381 ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat atgctgactg
     1441 aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc cttctgccat
     1501 aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta cgccgcgcca
     1561 gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc ggcgacctaa
     1621 tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa gaattaaagt
     1681 tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa aaccgccata
     1741 catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat agcgaccgcg
     1801 atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca gtaatgcgag
     1861 gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac caaatcgttc
     1921 gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg agccaaccca
     1981 attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca tcaaccatat
     2041 gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc gcacccacac
     2101 catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga atacttttgt
     2161 catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat tcatcagcag
     2221 cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg tctcgatggc
     2281 ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg gatagccaat
     2341 agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta acggcggaat
     2401 aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt tgggtaaccc
     2461 cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag cgcggataat
     2521 ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg ccagttcagg
     2581 aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat cccagtgtag
     2641 tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca ctgcggcgat
     2701 cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa agcggaatag
     2761 cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa atccgtctcc
     2821 cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat caaacgcgct
     2881 cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga agaagatttg
     2941 caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc cagttatccg
     3001 aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata cacaatgcag
     3061 aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg tcgccctgct
     3121 ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga aaggcaaaac
     3181 ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac caaccaacaa
     3241 tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc gtagggttgg
     3301 caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc tcggaaagca
     3361 taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac ttaacgcacg
     3421 ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg tttaattttc
     3481 agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc cacctgtttg
     3541 atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc gccttcttta
     3601 cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc gacgttctgg
     3661 gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg tacaatcgag
     3721 aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga gataacctcc
     3781 ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata cagttgcgac
     3841 tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga ttcgtcagag
     3901 cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc ggcgacgttg
     3961 agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc tttcgcgctt
     4021 tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt gacgccggta
     4081 aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg ggagagcaaa
     4141 tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg ggtatatttc
     4201 actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc aacatcaata
     4261 cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc actgatcccg
     4321 cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg cgcctgattt
     4381 gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt tttcataatg
     4441 cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt tacctgtctg
     4501 tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc gcgtcgcaat
     4561 aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DAJTRQ010000206         4375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000206 DAJTRQ010000000
VERSION     DAJTRQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..368)
                     /locus_tag="OO332_003979"
     CDS             complement(<1..368)
                     /locus_tag="OO332_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5944232.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(483..558)
                     /locus_tag="OO332_003980"
     tRNA            complement(483..558)
                     /locus_tag="OO332_003980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Thr,seq:ggt)
     gene            complement(565..639)
                     /locus_tag="OO332_003981"
     tRNA            complement(565..639)
                     /locus_tag="OO332_003981"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Gly,seq:tcc)
     gene            complement(756..840)
                     /locus_tag="OO332_003982"
     tRNA            complement(756..840)
                     /locus_tag="OO332_003982"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Tyr,seq:gta)
     gene            complement(849..924)
                     /locus_tag="OO332_003983"
     tRNA            complement(849..924)
                     /locus_tag="OO332_003983"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Thr,seq:tgt)
     gene            1286..2236
                     /gene="coaA"
                     /locus_tag="OO332_003984"
                     /gene_synonym="panK"
     CDS             1286..2236
                     /gene="coaA"
                     /locus_tag="OO332_003984"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5944233.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2265..3230)
                     /gene="birA"
                     /locus_tag="OO332_003985"
     CDS             complement(2265..3230)
                     /gene="birA"
                     /locus_tag="OO332_003985"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5944234.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3227..4255)
                     /gene="murB"
                     /locus_tag="OO332_003986"
     CDS             complement(3227..4255)
                     /gene="murB"
                     /locus_tag="OO332_003986"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5944235.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1121 a         1093 c          989 g         1172 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca
      241 gcaccgccgt aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca
      301 tggtcaacgt ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct
      361 ttagacatcg attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag
      421 gcaacatgcc cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga
      481 agtggtgctg ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tacgctctac
      541 caactgagct atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag
      601 cttggaaggc tgaggtaata gccattatac gatgcccgca tcctagaact cggctacctg
      661 attttcattc tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagtgaact
      721 tatcgtctcg ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc
      781 ttcgaagtct gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
      841 ggacttgatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      901 ctctacctac tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt
      961 catgcaacta aaaaattgca taatttgttt tattggtcac attttatgct acacgatgaa
     1021 gaaacagccg aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg
     1081 cgctcctgct aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa
     1141 cgatagcttc ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct
     1201 gcccattaac ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag
     1261 agctatgacc gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta
     1321 cctacagttt gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc
     1381 ggaagatgag atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc
     1441 cgagatctat ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg
     1501 tcaggcagtt ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag
     1561 tattgctggc agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt
     1621 aagccgttgg ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc
     1681 taatcaggtt ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga
     1741 tatgcatcgc ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc
     1801 ccctgtttac tcgcatctta tttatgatgt gatcccggat ggagataaaa ctgttgttca
     1861 gcctgatatt ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca
     1921 cgatccacat catgtatttg tttccgattt tgtcgatttt tcgatatatg ttgatgcacc
     1981 ggaagactta cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt
     2041 tacagacccg gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa
     2101 gactgccatg acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc
     2161 tactcgtgag cgcgccagtt taatcctgac gaaaagtgct aatcatgtgg tagaagaggt
     2221 cagactacgc aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta
     2281 cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa
     2341 gccccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt
     2401 ttcactgggc gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat
     2461 ccttcttgtt cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac
     2521 gtattacgat caagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca
     2581 ctctcttcaa cacggcgcat tgccatgttg atcccggatc caatgactat ttgcgccgca
     2641 tcgccagttt tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag
     2701 aggtcattag gccatttgac acgaacttta tctgctccca gctttcgtaa tacttccgcc
     2761 atcacgatac cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc
     2821 cagaacatcg acaaatataa gtttgcgcca aaaggcgaaa accatttccg cccccggcga
     2881 ccacggccag cctgctggta ttctgcaaca caggcatcgc ccgatttaag ctctccgata
     2941 cgatcaagaa ggaactgatt cgtggagtca ataactggca gcacggctac actaccgcca
     3001 tccagctgac ccaatatctg ttcagcatta agtaactgga tgggctcagg caggctgtat
     3061 cctttacccg gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta
     3121 ttaatagccg cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca
     3181 ccgttcgcta acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct
     3241 ccactgcgct cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat
     3301 taaatttttc acccactttt tgtcttacat ggtgtgccag ttgcaccaca tcttcgcttt
     3361 ttgcattgtc ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccca
     3421 tttgcatccc ttttagctgg cactgatcaa taagccaacc tgctgccagt tttactgaac
     3481 cacccgcctg ggggtaattt ggtgctgttg gaaattgcgc cagtaatgct ttagccgttt
     3541 cggcagatac aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag
     3601 ggagtttggt ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg
     3661 taggatccag acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac
     3721 gcagacctac ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc
     3781 gatagccaaa acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa
     3841 cacaatcaac ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga
     3901 taggtgatga gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt
     3961 cctgcaaagt gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc
     4021 acgcatcagg ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc
     4081 gatagtcttc cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt
     4141 ctgcggttgc atgctgccag gcattgagta attgttgttc gtcttcggca catacaatgt
     4201 gctgagcatt atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg
     4261 ctttcctgat gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata
     4321 tgacgacagg aagagtttgt agaaacgcaa aaaggccatc ctgacggata gcctt
//
LOCUS       DAJTRQ010000207         4358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000207 DAJTRQ010000000
VERSION     DAJTRQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..155)
                     /locus_tag="OO332_003987"
     CDS             complement(<1..155)
                     /locus_tag="OO332_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5944236.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLT"
     gene            complement(305..466)
                     /locus_tag="OO332_003988"
     CDS             complement(305..466)
                     /locus_tag="OO332_003988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944237.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(463..651)
                     /locus_tag="OO332_003989"
     CDS             complement(463..651)
                     /locus_tag="OO332_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5944238.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            911..1246
                     /gene="iraM"
                     /locus_tag="OO332_003990"
     CDS             911..1246
                     /gene="iraM"
                     /locus_tag="OO332_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5944239.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1527..1658)
                     /locus_tag="OO332_003991"
     CDS             complement(1527..1658)
                     /locus_tag="OO332_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR5944240.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2158..2234)
                     /locus_tag="OO332_003992"
     tRNA            complement(2158..2234)
                     /locus_tag="OO332_003992"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2198..2200),aa:Arg,seq:tct)
     gene            complement(2336..2411)
                     /locus_tag="OO332_003993"
     tRNA            complement(2336..2411)
                     /locus_tag="OO332_003993"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2376..2378),aa:Ile,seq:cat)
     gene            complement(2555..3376)
                     /locus_tag="OO332_003994"
     CDS             complement(2555..3376)
                     /locus_tag="OO332_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5944241.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3391..3747)
                     /locus_tag="OO332_003995"
     CDS             complement(3391..3747)
                     /locus_tag="OO332_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5944242.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3760..>4358)
                     /locus_tag="OO332_003996"
     CDS             complement(3760..>4358)
                     /locus_tag="OO332_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5944243.1"
                     /translation="AISGTQERELSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKI
                     RSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESF
                     MKRPKRRSWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCR
                     EHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1192 a         1187 c          862 g         1117 t
ORIGIN      
        1 tatgtcagca gcccaaacag cagactcccc agcacgccta ttgccgccca ctgagacggg
       61 gaaaacctgt ccagcaactg caggaaccag tagcccgttc ccaccgctga cgtggtgtat
      121 gacacacctg ttgtgatttt ttccatctgg tccatacccc gtctcccgtt atccggaagc
      181 tgacaacaat aaaaaagcca ccagttaact actgatggct ctgataactc atgcaagcgt
      241 ctcagacgac ccactgacac taccggtgag tttaacgata ccttccattt gactggctca
      301 ctttttatga tgatgccggt gcatttatct ccagcaccag actttctatc tcaacgccat
      361 acgttgcatt tttggtaata tccgtcagcg tcagtgcatt tagtcccact gccagactgt
      421 cttttatggc ctggaatgcc gggccagtac gatgacgtag tatcactccg gctcagttgc
      481 accactgacc accacatcac cttctgctgc aatcgcctgc atcagggtat aaggggttat
      541 ggccaccgga ctaccaaacg gctgccagcc ctctttcagt ttatgtgtca gcttttccgc
      601 aagatctgac ggcggcgccg ccctgacaac atcatagtgt ttaaatgcca tggttctttc
      661 cactatctga aaaataattc tttaaaatac ctgacatgta atacagaaaa aacacaaaac
      721 cataccttaa ataaaaacct gattatcaag cagatatgca tggataaact acaagacgag
      781 atataaacca ccctgcattt aaataaacaa taaacaacat cagaaaaata attctgctct
      841 atggtttaat tcaaaaatat catttatact tttcagaaca tcaccagcaa ggcataaaca
      901 aggaaactaa atgaagtgga ttgtgattga tacagttatc cagccatcat gcggaatatc
      961 tttttcagtc atatggagta aaataaaatt aataatctgg tatcaatcgg atgctttctt
     1021 acctcctgaa agtatattta cactgactca cacaggcatc atgctcaata acaaagtgct
     1081 acctgtaacc atttacaacg tagtaccatt caataaaaca ttctggaatt taatcaaaaa
     1141 cagccaggaa tgccctacaa atacagataa cgtattgaat gaatgcttta ataaccgttg
     1201 cactctgcaa atatgtcctt atggactaaa acaacaaagt ccataaggag tttactcaca
     1261 tctgacaaaa tcaatataaa cagcccctcc ggagaggggc tggagagtgg cgctatgtgc
     1321 cattgcatgg tgccgggtgc ctcccggtga attcagtacc agcacctgaa tccgcgatta
     1381 tcccatatac ctactcgctg attgcccctc cgcacagggg gattcaccat gccagtttct
     1441 tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc tgaattgtga agtatttaaa
     1501 aatttctccc gctaactgat acccggctaa cagtctggcg ttttcttttt cagcaacggg
     1561 aaagcaacaa ccaccacacc cgccaccagc acaccgtcag ccggcactga cattatccgg
     1621 ctgctgcaat gccattcaca aaaacagtaa gcaatcactt tttaccgtaa caggtgataa
     1681 tccagatatg tatctacccc agatgagtaa tccgaagttc atccatacca caggtcctgg
     1741 ctattctgtt gtactcctga acaagagcaa ataattctga attagcaacc atgaactcat
     1801 cgcaaaccct ctgtatagca tcactattca gaataataac gtctcttccc gaaagacgat
     1861 caggagtaca gaacaaaact gtcaaacggc tgaaggcctt tgctcgtgct gcattgacta
     1921 tatcaatacg ctgcctaagg atgaaacacc ccgacgcctc atcaatattc actctaccca
     1981 caccatatga atgataaata cttaatgctg aaaaaaccat tagaccgtat aacaaatact
     2041 caatcaatac ttaacagaac ttttattttt gacaaatata taatattttc aacaatatcc
     2101 tgagccaggt atatttcagt ataaggctct gccggaagga atctggaaga atgaatatgg
     2161 cgcgctgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc tctgtccggc
     2221 tgagctaaca acgctgaata ccgataatgg accgccatcg gggacccgcc ccccgcacca
     2281 acaaccctgt tatcgtgtcg tctgctcttc ctgataagct aatggcggtt tgtgatggtg
     2341 gcccttgctg gatttgaacc agcgacctgg cgattatgag tcgctcgctc tcaccactga
     2401 gctaaagggc cgggagcaga ataataacgg tccgtaatta atttcgcaat aaaaaacccg
     2461 ctcagcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca tcattatgac
     2521 gaatttacca cagattccgg aaaaatcaac cttgttacct agttaccttt tttaactgcc
     2581 gctcagccca tgcttcttca atatcaaacc gggtcaccag cgcatcataa aatttcttaa
     2641 ctgttttttc ccatgacgcg cgtgttatct ggtttgtcac ctcgcatata gcattaaatg
     2701 cctccgttga tggtagtctt tcatagccac gaccaccaca acgctggcag tctctgataa
     2761 caggcatacc acgttttacc gactcttcac gatgaatggc gacaccacgc ccacggcaat
     2821 ccttacaggc ggtggaaacc tcaccctttc cgccacactc cggacaggca acttttacca
     2881 cctccctgac ttttttccat tcttcccagt aagacggata cacacctttc gtacactttg
     2941 cccataccgg cggcttacca tccggatact ggatcttgtt tgtaaaaact atgctttcaa
     3001 taaatttttc cccatagcaa caagggcact gctttttact cgctgcgctg cgggcataat
     3061 cctcaaaagc gtacgaagcc ataatgcgca tcactaccgg ttttatttct gccggaagtt
     3121 ttctcaacgc cgccacacga tcgcaccgac tgagtgcata atctgccagt aattctgttg
     3181 cccgcgccct gtcattcata ctgatgccca ttttccccag gaacgcagaa aaacccatct
     3241 cagcccgatt ctgtgtcatg ccctgcgcgg ccatcacatc agtgatactc agcgcatctt
     3301 ttgacgttga ggccgatgca tcggtcaggc caggggattt tggggagtag tatttcggta
     3361 aatcttccag tttcattttt tgacctgctc ttaatgcatt atggggtaaa tcttcacccc
     3421 cagacgtcca ccagatactg gctgaccacg aacgatattg atttcatcaa actgctcatc
     3481 gtccattaac actcccgcat gcgtcagcgc atccagcggt gctttcagga tattgtccag
     3541 gtcgcgacga cgcttatccg gtggctctgc aatcaccttt atcgccagcc ttccggacag
     3601 gcttaatttc agccgctgct ggcgaacaat aagcgccaca gcccggcgat aacgctttcc
     3661 ctcctccgag ataaaatatg tgctgccacg gcgtcgccag taagtgttca ccgtcggcgg
     3721 gtaaggtaaa accaaatcta tgagcatcag tcacctcttt tacccaagca cgccagttgc
     3781 aaaggcgtga tcaagaaaac gaaaaattaa atcaacctga gaaccatgct tttcttcgaa
     3841 cgccagagga tccgcatgaa gctcgttgtg atgctcccga cacagcggta gcgtgaaaat
     3901 atcgtgagat tttgtcccca ttccgccctg accatgacca atcaggtgat ggggatcgtc
     3961 ggctggctta ccacaacacg cacacggctg tgtcttcacc cagcgtttgt atttctcgtt
     4021 aacccagctg cgacgtttag gtcgtttcat gaaagattcc ggagactcag gatcaacggc
     4081 aatgctgacc accgtctttt cctgttgtgg gttctgttgc tggtgggcgt gaggcagcgg
     4141 cgcaagattt tttgtgcgct gtttcagtat gctggtggcg gtctgctctc ccggtacgat
     4201 gtcgctttca cggtacattg agcggatttt ttccgcacgc aaccccagcg aacgacgtaa
     4261 taccgcttcc ggtagcgcgt ccgccacctg attgcggacc gcccaccagg ataattcagc
     4321 cagagataat tcacgctcct gcgtaccgct tattgcgt
//
LOCUS       DAJTRQ010000208         4288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000208 DAJTRQ010000000
VERSION     DAJTRQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            21..659
                     /locus_tag="OO332_003997"
     CDS             21..659
                     /locus_tag="OO332_003997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944244.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLPDWLPDTLWHEWVQFRQALRKPIRTELGANGAIRELEKFR
                     QQGFTPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            666..1412
                     /locus_tag="OO332_003998"
     CDS             666..1412
                     /locus_tag="OO332_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5944245.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1427..1849
                     /locus_tag="OO332_003999"
     CDS             1427..1849
                     /locus_tag="OO332_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5944246.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1907..2263
                     /locus_tag="OO332_004000"
     CDS             1907..2263
                     /locus_tag="OO332_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944247.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2356..2574
                     /locus_tag="OO332_004001"
     CDS             2356..2574
                     /locus_tag="OO332_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5944248.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2576..2938
                     /locus_tag="OO332_004002"
                     /pseudo
     CDS             2576..2938
                     /locus_tag="OO332_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2938..3174
                     /locus_tag="OO332_004003"
                     /pseudo
     CDS             2938..3174
                     /locus_tag="OO332_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3367..3603
                     /locus_tag="OO332_004004"
                     /pseudo
     CDS             3367..3603
                     /locus_tag="OO332_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3603..3968
                     /locus_tag="OO332_004005"
     CDS             3603..3968
                     /locus_tag="OO332_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5944249.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1126 a          901 c         1239 g         1022 t
ORIGIN      
        1 gaagggagtt tcgggatttt gtggctggag tgcttgaggg gagattatct ggcggtactg
       61 cagcggaatt ttgtaattct gcggtggttg cgttgcaggc tgctggcctg gatgtctgtc
      121 gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg gcggattgat atcgtcgtga
      181 ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg aaattctccg cgacagaaat
      241 cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt cttgcggcgc agtgatatcg
      301 caaggcacac cgagcaggga attctggtta tcggtggggc tgttcgccag aaaaaatttg
      361 acccgttgtc agttgatctg cccgactggt tgccagatac actctggcat gagtgggtcc
      421 agttcaggca ggcattgcga aaaccgattc gaacggagct gggcgctaac ggggcgatac
      481 gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt cgacacagca
      541 tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt tcggcctgag acgttgctcc
      601 gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc agggggtaac
      661 agaccatgaa aaatattgcg acaggaggcg ttctggagcg tatccgcaga ctgaccccac
      721 cacatgtaac cgccccattc agaacggttg cggagtggcg cgagtggcaa cttgctgaag
      781 gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca gttgcgggtg gaaaaaattc
      841 tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc gtttgcgaat taccgggcgc
      901 agaacgacgg tcagcgacat gcactgagtc aggcgaaatc catagctgac gaactgatga
      961 caggctgtac gaattttgtg ttcagcggta agcccggcac cggaaaaaat catcttgcag
     1021 ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt gattatcgtc accgtgtccg
     1081 atgtcatgag cgtgttgcat gagagctacg acaacggcaa atccggtgaa aaatttttac
     1141 aggagctttg tggtgttgac ctgctggtcc tggatgaaat tggcatgcag cgggatacga
     1201 aaaacgagca ggtggtactg aaccagattg ttgatcgccg gacggcatcg ttacgcggtg
     1261 tggggatgct gacaaatatt aaccatgcag cgatgaatac acttctcggc gagcgggtga
     1321 tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt taactgggag agctggcgtc
     1381 cgaatgttag ccattcgagg gttgttaagt agtttcagga ggatttatgg cgaaaccttt
     1441 tactcccgaa cagcgggaag aactgaagac gcgaattgtg gaactcgtgc atcaggacgg
     1501 tcgggtcacg attcggcagt tgtcagatga aacaggtatc agtcgtgcgt ctgtcggtcg
     1561 cttatgcatg gaactggtcg caagtggtga tgtatataat tctggctacg gggtatttcc
     1621 ctctgaacag gctcataaag actggcaaaa cgcccgcaaa aagctatcga gggtaaaggt
     1681 gaagaaaccg gttgtggttg atcctgacct tatctggtca ttaccagacg gagaaatacg
     1741 ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg aagctatgca
     1801 gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg gtactgtgag taaaattagc
     1861 tatcaggctt caattaccgc tggtattcgc atcaaaggag aggagcatgg aaataaaacc
     1921 agaagatgag ttaagcaata tcgttttatt tccggtaaaa gaggatgacc cacgtaatca
     1981 ggttaatttt ctttatgaac catcggaaag accatattgc catcacgcct ctgtccgggt
     2041 tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg agccgtttga
     2101 ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa ggctattgcg
     2161 ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg agcgtaacgc
     2221 gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt tagcactgtt
     2281 aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg agaccgcccg
     2341 aaagggcggt aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca
     2401 acagaattcc tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgctaatt
     2461 tttgtctgga taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta
     2521 tgggcgtcat tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc
     2581 actgacgaaa aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg
     2641 cggctgcgaa cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga
     2701 tggaaatatg cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc
     2761 atgcaatctg cacaagcatt gcagtagcct agaagattat cgacgaatta tcagtgatgg
     2821 tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga
     2881 aatgaaatct gacccggttg tgttctggtt tgaaaaatat caagaagggg ctacggcatg
     2941 gccactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt
     3001 gacgatatag acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa
     3061 gcagagcctg tagcggaatg cattgttgaa gatgggggca tgtgtattga cgggttcggt
     3121 gagtatgtgg gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg
     3181 cgacaacctg atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt
     3241 gaccaggaat ctaatacgtt cactgctata tattttgaca aagcggaagt tgagcgttgg
     3301 gtacgactgc ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg
     3361 gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat
     3421 tcatcgcttg gctctggtga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc
     3481 aacgtcacta tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat
     3541 acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa
     3601 taatgattaa tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta
     3661 ttgggcaact ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc
     3721 gtaaggctgg caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc
     3781 ctgctgaata ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct
     3841 gggataaggt tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg
     3901 gcgttgaagt agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga
     3961 atcaatgacc tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt
     4021 ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt
     4081 ttttgaagga aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta
     4141 aacgtatctc aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa
     4201 ggtgctggca ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct
     4261 tgaggctgtc tgcctcgggc atgccact
//
LOCUS       DAJTRQ010000209         4277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000209 DAJTRQ010000000
VERSION     DAJTRQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.213"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            160..829
                     /locus_tag="OO332_004006"
                     /pseudo
     CDS             160..829
                     /locus_tag="OO332_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1072..1767)
                     /locus_tag="OO332_004007"
     CDS             complement(1072..1767)
                     /locus_tag="OO332_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5944250.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1760..3187)
                     /locus_tag="OO332_004008"
     CDS             complement(1760..3187)
                     /locus_tag="OO332_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5944251.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3198..3917)
                     /locus_tag="OO332_004009"
     CDS             complement(3198..3917)
                     /locus_tag="OO332_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5944252.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1147 a         1100 c          880 g         1150 t
ORIGIN      
        1 ttccagatat ggggaccgaa tcgttatata aaacactgag gtaactctca tgcttcatat
       61 ccagtattca tgatgctggt tttttgctga tatttaaccc gaacattcct gatacagact
      121 attaatacaa cattatcttc gacagatttt agatatatta tgagtgaaca aatcaaacag
      181 gatatcgatc tgattgagat tttatttcat ctgaagaaaa agattcgtgt tatccttttt
      241 attatggcta tctgtatggc aatggtgctg ttgtttctgt atatcaataa agacaataca
      301 aaagtgattt acagccttaa aataaaccag acaacgccag gtatacttgt tagctgtgat
      361 agcaataata attttgcctg tcagaccaca atgactgaag atgttattca gcgaattact
      421 acattttttc agaccagccc agatatcaag aacagagaaa taaggctgga atggtcagga
      481 gataagagag atttaccaac tgctgaagag gaaatatctc gcgtgcaggc gtctattatc
      541 aaatggtatg cgtcagaata ccataatggc aggcaagttc tcgatgagat acaaacgcct
      601 tcagcaatta acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg
      661 tatccgaacg gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct
      721 gctgccattt gcctagctct gggatttttt tctaagcatt gtaatttctg taatgttttg
      781 ccttgtcaaa aaaatggtag atgaatacca acaaaactct gggcagtaat aaacagcatt
      841 ctgatagaga gtgcctgtat aaagctgaag tgacaatgac cgggaaggga acccgttttc
      901 aggaatggat gcctgtcttt ttcactgtaa atatcctggc attactaatg cctggatatt
      961 tacagaagag cagcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat
     1021 tttcaacctc gtatgccttc ttcaggttta tgttcagatc tcatatctct ctcagcaatc
     1081 ctcaataatc agatacaccg ctttcaccgg accatgaacc ccgacgactt tgataagctc
     1141 aatatccgcc gttgagctgg gaccactgat gatgttaata catgacggca ttcgttcacc
     1201 ggcctgcgct ttctgatgca atttttctgc gagttgcgct acacgcggca ggattgtgct
     1261 tttacgcagg ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgctcggc
     1321 ggcggaaaaa agaaccacgc ctcccgattc ggttaaacca tattcagcat acacgacacc
     1381 cactttagcc tgctctgcct gcgagatatt ctcggcacct ttcgccggat cccaaacaac
     1441 ggcattgcat tcctgctgca aacgttcact aatccccaat tcctccagcc tcgtgtcacc
     1501 gctaatcacg accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc
     1561 cttcgcctcg ctggtcagct cacagcgcgt caacataaca tcgctggcaa actgaataaa
     1621 cgcgtcacag cgctgctgtt ggttaagttg ggtaagccgc tcgttagcat agttgttaag
     1681 cggcgcatct tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct gagcaacgtt
     1741 attcaaaaat tcgctccgat tatccattct ttttctcctg cgcctgatgt ttcttaaacc
     1801 aactacggaa actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa
     1861 tcgcgccaaa tttgagtggt gttttaccgc cattgataaa ccagcttgcc gcatgagcgc
     1921 cggccatcat cccgactttc cacaatcctg gatgactatt ggcataagcg aacattttta
     1981 tcgcccgttg ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca
     2041 aaatcagttt tgacagcgga atacgcaccg gacacacgct gtcacaagca gtgcataaag
     2101 agcaggcgta gggtaaatct ttaaaatctt tatagccgcc aagtagcgga gaaatcaccg
     2161 caccaattgg ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg
     2221 gacaagtatt catacaagcc ccgcagcgaa tacagcgcag cacatcccga aattcagagg
     2281 ccagcacctc agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa
     2341 cgtgccccgc ttcgcgcggc cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa
     2401 cggcactgcg cgccagcatg gtgatcaata catctacctc ggcaaacgtg ggggcaatac
     2461 gctccattcc catcactgca atatgcgttt taggcagcgt ggtacacatt cgcgcattac
     2521 cttcattggt caccaggcat accgaaccgg tctctgccac cgcgaaatta cagccggtaa
     2581 tacctatttc agcactgagg aaatcttcac ggattttttg ccggatgaat aaggtcatcg
     2641 cttcaggcgt ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat
     2701 ggcgatcttt atgaattgcc gggaccacaa catgagatgg cggatcttga tccagctgga
     2761 gaatatattc acccagatcg gtttcaatca cctgaatgcc agcatcctgc aacacatgat
     2821 tgacaccaat ctcttcggtc accatcgatt tagatttcac caccttccgg gcatttttgc
     2881 gttgggcaac ctgtaaaatg tagcgggtag cgtcttcttt ggtttttgca aaatagacgt
     2941 gaccgccgtt ttgcgtcact ttttctgaga gctggtacag ataagcgtcg agattactca
     3001 aaacatgatc acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat
     3061 cgaccatttt ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttgcgca
     3121 tgatcggatc ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga
     3181 tcgacatctt tatatcctca gcggctcatc aacacttcag caatatgcat cactttgact
     3241 ttctgccctt cccgttgtaa tcgcccactg atgttcagca ggcaactcac gtcagcacca
     3301 attaaatact cagggcggac ttccatcagg tgcacaacct tttctttcac catctcgccg
     3361 gatatttcgg ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca
     3421 gcaaaggtca acagctccag tccacgcaca tttttcagca gcgtaagtgg ctcgtccttc
     3481 actcccagct tacgggccag gctacaagat gggtgataca cagctctccc ttgcaaactg
     3541 gcacctacat cgactacccc taatttatta acaataaaag aggtgagatc ctgcatacgc
     3601 gcggcaacct tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa
     3661 ctttttacgg cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc
     3721 agtgcggcga tcagattttt catccctgga atcgcttctt tgatataacc gctattgatc
     3781 gcaggctgac cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc
     3841 agtagcagca cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca
     3901 aagaaattga cattcacaac taatactcca ttacttcatg cccatttatg cccgatgttt
     3961 cccccccacc gggggatcaa tcatcaataa atatggcgtt ctttttaaat tggctagcaa
     4021 cataccaccc ctcatttgta tgacaaatga taatctttca tccaaacttc aaatgcaata
     4081 actaatttta tttctttcgg catcgctcac attttatggg gaaatcgacg atcatttttg
     4141 gcataagaaa tccatttaaa aatggctaca tcccttatac ctattcagag ctacttattc
     4201 aggggttatt gagatataca ctgatggtgt acaaaaaaat caccgacaat aatcacgtat
     4261 caagtaatag tcggaat
//
LOCUS       DAJTRQ010000210         4235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000210 DAJTRQ010000000
VERSION     DAJTRQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..324
                     /locus_tag="OO332_004010"
     CDS             <1..324
                     /locus_tag="OO332_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5944253.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="OO332_004011"
     CDS             383..586
                     /locus_tag="OO332_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR5944254.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            586..1611
                     /locus_tag="OO332_004012"
     CDS             586..1611
                     /locus_tag="OO332_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5944255.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="OO332_004013"
     tRNA            complement(1664..1753)
                     /locus_tag="OO332_004013"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="OO332_004014"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="OO332_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5944256.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="OO332_004015"
     tRNA            2745..2820
                     /locus_tag="OO332_004015"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..>4235
                     /locus_tag="OO332_004016"
     CDS             2982..>4235
                     /locus_tag="OO332_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5944257.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKF"
BASE COUNT         1176 a          971 c         1023 g         1065 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa
      541 aactttacca ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatatccctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggccgt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agttt
//
LOCUS       DAJTRQ010000211         4120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000211 DAJTRQ010000000
VERSION     DAJTRQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            62..1371
                     /gene="ltrA"
                     /locus_tag="OO332_004017"
                     /pseudo
     CDS             62..1371
                     /gene="ltrA"
                     /locus_tag="OO332_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1548..1982
                     /locus_tag="OO332_004018"
     CDS             1548..1982
                     /locus_tag="OO332_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR5944258.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            2056..2175
                     /locus_tag="OO332_004019"
                     /pseudo
     CDS             2056..2175
                     /locus_tag="OO332_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2308..2478
                     /locus_tag="OO332_004020"
                     /pseudo
     CDS             2308..2478
                     /locus_tag="OO332_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2489..4108)
                     /locus_tag="OO332_004021"
     CDS             complement(2489..4108)
                     /locus_tag="OO332_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5944259.1"
                     /translation="MFTLSLLTGNPAIAYETDKVYKITVLHTNDHHGHFWRNNHGEYG
                     LSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIRGMNLIGYDAMA
                     VGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIRQNLKIAVIGLT
                     TDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHMGHYDNGESGSN
                     APGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCVPDKQNGIWIVQ
                     AHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSFYTPEITEDPQM
                     LSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSALTERIDADFAV
                     VSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATVAQMKPDSGAYP
                     QFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPRIDNRPGYINTG
                     FIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
BASE COUNT         1100 a          940 c         1012 g         1068 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcaac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcaaaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg atcgcaaact cgtgttatgg ctgccccgta aacacaagcg acttaggggc
     1261 caccgacgac gggccagcca ctggctggcc cgtgttgcgc ggagtgaaac gaggttattt
     1321 gcacactggc cgttactatg gggacaggcc tcaatgagaa gagccggatg agctgagagg
     1381 ttcacgtccg gttccgtgag caccagaggg ggaggttccc tcgggtgact cgacttgtag
     1441 tacattgttc acgaatgagt gatgcaacaa ggctgaagaa caggttatcg gtgcgcttct
     1501 cggaagtcgg gttggtcctg aacgcaggga agacgaatat cgcctacatt gacacgttta
     1561 aaaggagaaa cgtcgctacg agtttctcct ttctcggata tgacttcaaa gtgcgtactc
     1621 tgaagaattt caaaggcgaa ttgtacagaa aatgtatgcc gggtgcatca aatgcagcaa
     1681 tgtgcaaaat aacagaaaca atcaagaagt ggcgtataca tcgctcaaca gctgagagtt
     1741 tgctggattt tgcgaggcgc tacaatgcga tagtgagagg ctggattgag tactatggaa
     1801 agttctggtc cagaaacttc agctatcgac tgtggagtgc aatgcagtca cgtttgctta
     1861 agtggatgca gtctaaatac agactttcga accggaaggc tcagcgaaag ctggcgctgg
     1921 taaggaaaca gtatccgaag ctatttgccc actggtattt actacgtgca tcgaatgagt
     1981 gatcaagagc cgtatgacgc gagagtgtca cgtacggttc cgtgagcagc cggagagtga
     2041 aactctctcc ggcggggttc cgatagcggt ggcgaacatg cggcggtgtt gtactcgctg
     2101 atcggcacat gccgtctgaa caatgtggag ccagaaaaat ggctgcgtta cgtcattgag
     2161 catatccagg actgaccggc aaaccgggta cgcgatctgt tgccctggaa agttgatctg
     2221 agctcttagt aaatatcaat atggttctga cgagccgctt acgcagatca tgccataatg
     2281 aataaacttt aatcagcgaa attgacgaag aagctggcag cgctccaaca tcagttaagc
     2341 cgcaaggtta aattcagcaa caactggcag aagcagaaac acaaaataca gcaactgcat
     2401 tcccatatcg caaacatccg cagagactac tttcacaaag tcacagcgac tgtcagcaaa
     2461 aaacacgtga tgatcgtcaa ttacgttatc actgccaact gacttcacct tttggttcat
     2521 atgaagcagc atccagcggt gaatgtttcc ggatatattc gataagtact tcagcatcaa
     2581 taaatccggt atttatgtat ccgggtctat tatcaatacg tggatagcca tccccaccag
     2641 tagcattaaa gctaaatgtt gtcatacgat attttttgtt aaggtcaacc gatttacctt
     2701 ttatttttag catttcgatt tttccttttt tgactacaaa gctggtattc aaaaattgtg
     2761 ggtaggctcc ggaatccggt ttcatctgtg ccactgtagc taggtagtca gctacttctt
     2821 tccccgtcaa atcaatactt actaccgtat taccaaatgg ctgaactttc agtatatctt
     2881 tgtatgtgat gttaccactc tctatggaat ctcgaatttc cccaccactg acaacagcaa
     2941 aatctgcgtc aatacgttct gtcagagcgc tcagaatcag atggcccata ctggtctgaa
     3001 caaaacgaac tttacttctg tccccttcca gacgtccgtt cacaacccca accttcacat
     3061 ccagttgtgc tttgccttta ttctggaatg gtgtcagtaa ggataacatc tgtggatctt
     3121 cagtaatttc gggggtgtaa aaagaaaatt ccgtttttcc atcttctctg gtaattctca
     3181 ttttgagatt aactgggtgt aactgataat taaccagttt cattgtaccg ttacagaatt
     3241 caaaatcagc ctgaccgaca tattttcccc actcatgcgc ttgtacaatc cagatgccat
     3301 tttgtttatc tggtacacag gttgtccccg gaatataatt ccactgtttt ttattatcgc
     3361 tggccatgca gacaggagct tgtgaatgtc ctcccacaat cattgctaat gagccagtcg
     3421 gcaaactgcg agccatttca acatcaccag gagcattcga acctgactcg ccattgtcat
     3481 agtgacccat atgtgttgcc gcaataataa tatctggctt ttcctgctga ttaagttcat
     3541 caatgacact cctcgcctca gcagcaggtt gacggaactc aatatcagta aaatattcag
     3601 aatttcctgt tttcgcagta tcatctgtcg tcaagccaat gactgcaatt ttcaaattct
     3661 gacggatgaa aattttccat ggactgaata aacgcctccc tgtacttttt tgatatatgt
     3721 tagcagaaag gaaaggaaat gtggcccatt tttcctgctg acgtaatata tttagaggat
     3781 tatcaaattc atgattaccg acggccattg catcataacc aattagattc attccacgaa
     3841 tatcgggttc agccttctga aggtctgatt caggtacacc ggtgttaata tcaccaccag
     3901 ataataatag aacacttccg ccattattta ttactttctg ccggatattg tccaccagag
     3961 ttttttggga ggataacccg tactcaccat gattgttacg ccaaaagtgt ccatggtggt
     4021 catttgtatg cagaacggtt attttatata ctttatctgt ttcatacgct atagcaggat
     4081 ttcctgtcag taatgacagg gtgaacatta ttagcgtaat
//
LOCUS       DAJTRQ010000212         4092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000212 DAJTRQ010000000
VERSION     DAJTRQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(321..503)
                     /locus_tag="OO332_004022"
     CDS             complement(321..503)
                     /locus_tag="OO332_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944260.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            834..1706
                     /locus_tag="OO332_004023"
     CDS             834..1706
                     /locus_tag="OO332_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5944261.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1924..>4092
                     /locus_tag="OO332_004024"
     CDS             1924..>4092
                     /locus_tag="OO332_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR5944262.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDIN"
BASE COUNT         1085 a          987 c         1056 g          964 t
ORIGIN      
        1 ctgagcggga ggggaagatg atttcactcc catcaggtac ccgtatctgg ctcgttgccg
       61 gtgttaccga tatgtgtaaa tctttcaacg gatacgacat atgctgaagg aggatctgta
      121 atggattaag tgataacaga tactcagaaa tataggggca aatccactat tttgatgaga
      181 attattatat gttacctcgg attttaatca ggcgccaaga ttaaataaaa tattgagcgc
      241 ctgattatat ttattcattc attcattcat tcattaatta atatctgtta ttgattggct
      301 tactatcagc ttcccccgta ttaatcagag ctttcattta tttttagtgc ataatcagat
      361 gccgcctcat cattatcctc actattatca tagccgaaaa cttcccccgg acacgggcgt
      421 tcacgataat agtcatcgtc atagttttca tcgtcagata atgatatgtc gtattctgaa
      481 tatgattcac cacaattata catgaaatca tctcctttgg ggtggttaca ataattagtg
      541 agtttattcg aaaaaatgat gagcgccaga ccaacccacc caacttttga ttataatcat
      601 gaagatactt gtctcagtac aaatagaatc aaactcagtg cagcatgatt cacctttata
      661 ttctctcagg gaaaccaaat accactactc aactattaag aacaaaattc tggaggtatg
      721 attatctgaa gatacctgta tcctaaataa tttcagacag atattatcgt tgtgagtccc
      781 ccgccacctt tccggtgcgc ggggttttgt cttttttcac cgggaataca tgtatgaatc
      841 cgtctgatgc cattgaggca attgaaaaac cgctctcctc cctgccttac tcactttccc
      901 gtcacatcct ggaacatctg cgcaaactca ccagtcacga acccgtgatt ggcattatgg
      961 gtaaaagcgg ggccggtaaa tcttcactct gtaatgcact gtttcagggg gaggtcaccc
     1021 cggtcagtga tgttcacgcc ggcacccggg aagtacggcg cttccgtctg agtggccatg
     1081 gtcacagcat ggttatcact gacctgcccg gggtgggcga gagccgggac agggatgcag
     1141 agtatgaggc cctgtaccgt gacattctgc ctgaactgga cctggtactg tggctgatta
     1201 aagccgatga ccgtgccctg tctgtggatg agtatttctg gcgacacatc ctgcactggg
     1261 gacatcagcg ggtactgttt gtggtgacgc aggccgacaa aacggagccc tgccatgaat
     1321 gggatatggc cggtattcag ccttctcctg cacaggcaca gaatattcgc gaaaaaacgg
     1381 aggcggtatt ccgtctgttc cggcccgtac atccggttgt ggccgtatcg gcccgcactg
     1441 gctgggaact ggatacgctg gtcagtgcgc tcatgacagc gcttcccgac catgccgcca
     1501 gtcccctgat gacccgcctg caggacgggc tgcgcacgga atctgtccgg ggtcaggccc
     1561 gtgaacagtt taccggtgcg gtggaccgga tatttgacac ggcggagagc gtctgtgttg
     1621 cctctgttgc ccgtaaggtt ctgcgcgctg ttcgtgacac cgtggtctct gttgcccgcg
     1681 cggtatggaa ctggattttc ttctgaaccc ggtggtgatg caccagactc ttgttacaga
     1741 tttttaaccc catgaaaaca caatgcaaac acagccatac ccgggttgtc cccttgcgtt
     1801 gtgtttctca agttaataga atgcatcgat cgttactatc atatcgatcg ctataaacga
     1861 tcgtttgtgg taaatgacct cctccgtggt tgtcatgaaa atgcgtctca ggaaggttaa
     1921 ataatgcacg aaaagaacat cgccctgctt tgtgatgaag ccgaccgact tttgcaactg
     1981 aacattaatc tgctccggca aatggttgat gaaccggatg tgttatctga cagtaagaac
     2041 gaaaacagac tgctttttga taaacagaaa gcactgaaaa gaattgagga gctggagggc
     2101 gaacaaatca aaaccgcccg cagggagatg gtgctggctg ttgtcggcac gatgaaagca
     2161 ggtaaatcaa ctaccatcaa cgccattgtg gggcaggaaa ttctgcctaa ccgtaaccgc
     2221 cccatgacct ctgtaccgac gctcatccgc cacgttcccg gaaaaactga gccggtcctc
     2281 catctggaac atattcagcc cgtccgcaat ttattaagca cactgcaaca aaaactcgac
     2341 accccggcag gacagcaagt cgcacagacc ctgcagcaaa ccggggatac ctgcgaactg
     2401 ctggatattc tggcaaatga tggctggctc aaaaatgaat accacgggga agacgaaatc
     2461 tttaccggac tggcatcgtt aaacgatctg gttcgccttg ctgcggcaat ggggagtgag
     2521 tttccttttg atgaatacgc agaagtgcag aaactgccgg tgatcgacgt ggaattcagc
     2581 catctggtgg ggatggatgc atgccaggga acactcacac tgctggatac ccccggccct
     2641 aatgaggccg gacaaccgca gatggaagtg atgatgcggg atcaactgca gaaagcctct
     2701 gcggtgctgg cggtgatgga ttacacccag atgaactcaa aagcggatga agacgtccgt
     2761 aaagagctta atgccattgc tgacgtatca gccggccgcc tgtttgtgct ggtcaataaa
     2821 tttgatgaga aagaccgcaa tggcgatggg gcagatgccg tacgccagaa agttccggca
     2881 atgctgaaca gcgatgtgct gcccgcctcc cgcgtttatc caggctcatc acgtcaggcg
     2941 tacctggcta accgtgcgct tcatgagtta cggaaaaaca gaacccttcc tgttgatgaa
     3001 gcctgggtcg atgattttgt cagggaagcc ttcggtcgca tgaaaaaaga atacgtctgt
     3061 aaagacagtg aaatggcaac cgaaggggcg acagacctgt gggaaggctc acttatcgat
     3121 caactgataa cggaagtcat acagagctcc cattccagag cggcggcact ggcggttgat
     3181 tctgccgccg caaaactgat gcagaatgcg gaaaatgtca gcgaatatct gtcattacgc
     3241 catcaggggc tacagcaaag cattcagtca ctgcagtcgc atatcaccag cctgcttgcg
     3301 gatatccggg aaatcgcgga ctgtcaggag caggtgaccg ctgatgtcag aatggccatg
     3361 gaggagatcg atgccagaac ccgggaatta ctgacggggg tctgcacctc actggaagag
     3421 gagctgaatg actatttcag aagcggtaaa cgcaaagaac agcaaatgct ggaggaagaa
     3481 aattcagcgc agccccggga gagaaacgca ttcgctttct ttcacgatat tttcggaacc
     3541 ggaaatcagc atgacaggat gcgggacttt gatccggaca gccccgaaat aaaattcagt
     3601 gatcgcaggg aagcccttga actgatgacg caaatcgaat cgaccgtgac cagcctgcac
     3661 cgtgaggctg aagcacagtt ccgtcctgaa ctggaaaaaa tcgtcagggg tattgaaaca
     3721 ggttttcgtg gtacggccct gtacgccaca gaaaatattg ccggacgcat caatgcccgt
     3781 ctggaggatg aaggtttcac tgtaaaaatc agttttccgg cagtcagcca gttacagacc
     3841 cggctcgcag taaaaacaaa tctgagcgcg cttatggagg aaagaacgga gacagtaacc
     3901 cgtcgctgtc gacaggatgg tgtctggggt acgctttgtc gatgggcaaa caccagtgac
     3961 tggggctgga aagagtacag tgtggatgtg agccgcagcg tgatcaatat caacaaggtc
     4021 agagaggaag tgatgtcact gacccgggca tatttcgggg agctgcaggc atccattgag
     4081 caggatatta ac
//
LOCUS       DAJTRQ010000213         4076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000213 DAJTRQ010000000
VERSION     DAJTRQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..485)
                     /locus_tag="OO332_004025"
     CDS             complement(<1..485)
                     /locus_tag="OO332_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5944263.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
     gene            complement(696..2093)
                     /gene="ccp"
                     /locus_tag="OO332_004026"
     CDS             complement(696..2093)
                     /gene="ccp"
                     /locus_tag="OO332_004026"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5944264.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2498..>4076
                     /locus_tag="OO332_004027"
     CDS             2498..>4076
                     /locus_tag="OO332_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5944265.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEY"
BASE COUNT         1052 a         1041 c          950 g         1033 t
ORIGIN      
        1 accggaccgc acactaggtt tggtttatcc gttggtttgc ctgcagcttc catacgcttg
       61 cgccaacgcc atttcatcgc catcccgccg agcatacagg cctcggaaga accaatggtg
      121 ttggtgccaa cggcctgacc atttttcggc gcaggcgcat gccacagatc ggcaaccata
      181 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
      241 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      301 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      361 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      421 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      481 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      541 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      601 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      661 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      721 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      781 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      841 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      901 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      961 tccccaaagt taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata
     1021 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
     1081 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
     1141 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1201 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1261 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1321 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1381 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1441 acgccccccg cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1501 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1561 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1621 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1681 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1741 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1801 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1861 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1921 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1981 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     2041 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     2101 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     2161 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2221 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2281 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2341 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2401 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2461 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2521 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acctgtatga
     2581 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2641 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2701 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2761 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2821 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2881 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2941 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     3001 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     3061 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     3121 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3181 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3241 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3301 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3361 cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3421 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3481 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3541 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3601 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3661 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3721 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3781 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3841 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3901 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3961 aatcgcgcgc agttggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     4021 cgagaaatac catattgccg atggtgtttc ccgcgaaggc ggcggtggcg agtatc
//
LOCUS       DAJTRQ010000214         3972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000214 DAJTRQ010000000
VERSION     DAJTRQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..203)
                     /locus_tag="OO332_004028"
     CDS             complement(<1..203)
                     /locus_tag="OO332_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5944266.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO332_004029"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO332_004029"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5944267.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO332_004030"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO332_004030"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5944268.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO332_004031"
     CDS             2884..3624
                     /locus_tag="OO332_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5944269.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..>3972
                     /gene="ruvC"
                     /locus_tag="OO332_004032"
     CDS             3659..>3972
                     /gene="ruvC"
                     /locus_tag="OO332_004032"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5944270.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AAR"
BASE COUNT          976 a          983 c         1116 g          897 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cg
//
LOCUS       DAJTRQ010000215         3877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000215 DAJTRQ010000000
VERSION     DAJTRQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..203)
                     /locus_tag="OO332_004033"
     CDS             complement(<1..203)
                     /locus_tag="OO332_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCR5944271.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="OO332_004034"
     CDS             complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="OO332_004034"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5944272.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2926..3681
                     /locus_tag="OO332_004035"
     CDS             2926..3681
                     /locus_tag="OO332_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5944273.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3760..3833
                     /locus_tag="OO332_004036"
     tRNA            3760..3833
                     /locus_tag="OO332_004036"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3792..3794,aa:Gly,seq:ccc)
BASE COUNT          971 a          957 c         1014 g          935 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt
     1561 gttagacaga acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg
     1621 cccgtcaata gtaaaagcgt ggcgggtatg ggttgcgagg aaacactctt cacggctaag
     1681 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
     1741 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
     1801 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
     1861 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
     1921 catgtgacaa tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc
     1981 atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc
     2041 gttatggatt ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa
     2101 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
     2161 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
     2221 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat
     2281 atcaggcaca tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
     2341 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
     2401 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga
     2461 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
     2521 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
     2581 cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga
     2641 tatgatacga aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata
     2701 aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca
     2761 ggaacttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
     2821 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
     2881 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
     2941 ctgaataaaa aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc
     3001 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
     3061 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
     3121 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
     3181 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
     3241 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
     3301 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
     3361 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
     3421 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
     3481 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
     3541 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
     3601 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
     3661 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
     3721 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag
     3781 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact
     3841 tcttaaagtt caataactta ctgaatcata agggata
//
LOCUS       DAJTRQ010000216         3773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000216 DAJTRQ010000000
VERSION     DAJTRQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(12..869)
                     /gene="sitD"
                     /locus_tag="OO332_004037"
     CDS             complement(12..869)
                     /gene="sitD"
                     /locus_tag="OO332_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5944274.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(866..1723)
                     /gene="sitC"
                     /locus_tag="OO332_004038"
     CDS             complement(866..1723)
                     /gene="sitC"
                     /locus_tag="OO332_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5944275.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1720..2547)
                     /gene="sitB"
                     /locus_tag="OO332_004039"
     CDS             complement(1720..2547)
                     /gene="sitB"
                     /locus_tag="OO332_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5944276.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2547..3461)
                     /gene="sitA"
                     /locus_tag="OO332_004040"
     CDS             complement(2547..3461)
                     /gene="sitA"
                     /locus_tag="OO332_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5944277.1"
                     /translation="MHSIKKVTMLLGCLALTCSITFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1039 a          936 c          911 g          887 t
ORIGIN      
        1 cggatgttgt gctaattggg tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt
       61 gcgttgatgc tggtaacggt aaacgtcatg atgaacacga ccgcgaataa gacgacaatc
      121 gtcggagctg gtgcgctgtc gataaaaaag gacagccaga caccagtcat tgacactatt
      181 accgatatgc cagtcgccag taggagcgca atatgaaagc gttgtgtcag gagtactgca
      241 atggcgccag gcgcaatgag cagagaaata gagagaatga tacctactgc tttcagcgtc
      301 gccacaatgg tcagggaaac catgcacagc agcccataat gcagccatct cgtatgcaaa
      361 ccgcttacct gcgcctgttg ataatcaaag ctgaacagca aaaaatcccg ccattttacg
      421 ctaataacaa gtgtaaccag cccggcaata atcaccgtct ggattatatc gccgatggtt
      481 atcccgagca tgtcgccgaa aagaatatgg tcaagatgta cgtctggttt aattgcaata
      541 tacaagatca gacctgcggc aaacatgcca gaaaaaacaa tccccataac tgtatcttgc
      601 ttgattcggc tgttgtcttt caggtatccc gtcgccaccg cgcagaatac tccggcgacg
      661 aatgcccctg tagccagagg taaccccaga atccaggtaa gcacgatgcc aggaaaaacc
      721 gcatgactca tcgcgtcacc catcagtgcc caacctttca ggaccagaaa gaccgacagc
      781 agcgcacagg gtatggaaac taccagtgaa atcagcagtg cgtgactcat aaaggtgaac
      841 tgcagtggct ccagtaataa tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc
      901 gacgatttgc caaaagtcca tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg
      961 tctgcgccac cacgatgatt ccacctgtcg cgccgtccag aaagtaactg acccacgcgc
     1021 cgaggaaact ggttacactg ccaatagtta cagcaattat gagcaggcgg ggaaaacgat
     1081 cggttaacaa ccatgctgtt gcaccgggag tcaccaccag acagatcacc agaaaggcac
     1141 caaccgtttg cagcgccgca acggtcgaaa ccgacagcag ggtgaagaag aggattttta
     1201 atctgcccgg atgtaatcca atagcacggg cgtgattctc gtcgaaaaag gtcaccataa
     1261 gatctttcca tttgaaaaaa agcacgatga tcgagagaat gccaatgata gtgagttgca
     1321 ggatatccgc cggatcgatt gccagaatat tgcccaggac gatggtctga atgttcacgg
     1381 atgtcgggtt caatgacacc ataaacagcc ccaggccaaa aaaggacgag aagatcaggc
     1441 cgatgatggc atcttctttt aagcgggtgc gttggttaag aaacagcatg ctgcccgctg
     1501 ccagtccgcc ggaaaagaag gctcccagag aaaacggaag ccccagcata tatgcccccg
     1561 caacacccgg gacaatagag tgcgacagcg catcgccaat cagcgaccag ccttttagca
     1621 tcagatagca tgacaggaaa gcgcagagac cgccgaccat tgccgagacc cacatcgcat
     1681 tgagcatata ttcatagctg aagggttcca gtagcacgtt catcttgctt ccctctgcac
     1741 cgctgacggt cgatgtgcca caaaagggcg ttcatcgtca gtaataatgc tttcttccga
     1801 gccgttaagt gtgacatggc gcagtacgcc gctaaaagcc agctccaggt tctcggctgt
     1861 aaaagtggtg tctgtcggcc cgcttgccag gacggtgcct ttgaccatga ccgtataatc
     1921 gcaaaatgtc gtgactgagc cgagattgtg cgttgagaca agcatcgttt tgccttccgc
     1981 gcgtaactcc ctcaacaggc tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa
     2041 aggttcatcc agcaggatca catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg
     2101 tttcttttgt ccaccggaaa gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac
     2161 acgttccagc gcatcggtga caatctgacg atccctcttt ttggctatac gcaacatacc
     2221 catatgccca tagcggccca tcatcaccac atcttccacc agtacaggaa atgaccagtc
     2281 aacctcttct gactgaggga cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg
     2341 aatacccaga acagatatct ttccgctggt cagacgcaca aatcccataa tcgctttaaa
     2401 caacgtcgat tttccggaac cgtttacccc aaccagagcg gcaatagatc cacttggtac
     2461 tgtgaaggat gcatcgcgca gtgctgtgtg cccgttacgc caggtgacag taacatcatt
     2521 aacgacaatg cctgcagatt gcatcattat ttctcccttt tcccggcttt aattccctgt
     2581 accagcgtac tggtagtaac cttaagaagg tcgatatacg taggtaccgg gccgttttct
     2641 gtgctcaggg aatcgacata gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc
     2701 tgacgcgctg gtttatcgga aatcgtactc tcgctaaaga ctgccgggat atgatttttc
     2761 ttaactatat caacaacctt acgtacctgc tgcggtgttc cttgttgatc ggcattaatc
     2821 ggccacagat aaagctcttt tagccccaaa tcgcgtgcga gataagaaaa agccccttca
     2881 ctggtgacca tccatcgctg attctcaggg agttccgtaa tctgcttacg caggggggca
     2941 agggtttggg taatcttggc tttataagta tcggcattac gttggtaggt ttgtgcattt
     3001 gccggatcgt attttatcag cgcatcacga atattatcga cgtaaatcag agcattatct
     3061 ggcgacatcc atgcatgggg attaggtttg ccctcatagg gtccttcggt gatccctact
     3121 ggcgtcacac ccgaagagac aattacttct ggaaccccat tgagatgctg gtaaaagcgt
     3181 tggaaccaca attccagatt cataccattg gcgagaatca gttgtgcccc ctgcgcacgt
     3241 ttaatatcgc caggggtagg ctgatactca tgaatttctg caccaggctt ggttatggat
     3301 gagacttctg cagcatctcc agccacgttt ttggccatat ccgcgatgat ggtaaatgtt
     3361 gtaatgacct tgaatttttc agttgcactt gcctgaaatg tgatcgagca ggtgagggcg
     3421 aggcacccca agagcatggt tacttttttt attgagtgca taatttagta cctatagtga
     3481 gcaaaattag aacaaacata gcaacggcta tgttttatag cataacctat acaaaatgaa
     3541 aataattatc attaacagct ttgaggccta aaacagggat taggttgttt cctgctggcg
     3601 acatgatatt gaggaaaaaa gtagagcagc tattaggctt tgaaatgact ttatgaggaa
     3661 aattgttgat ggcaaaaatg gggcaaaacg ctgtataagg ggcaaaaaag gggcaaaaaa
     3721 gagtgggtta tcgtagctta ttgttgtcgc tgataatgtt taacatattg aaa
//
LOCUS       DAJTRQ010000217         3738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000217 DAJTRQ010000000
VERSION     DAJTRQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.221"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            108..2105
                     /gene="betT"
                     /locus_tag="OO332_004041"
     CDS             108..2105
                     /gene="betT"
                     /locus_tag="OO332_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5944278.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="OO332_004042"
     CDS             2168..3445
                     /locus_tag="OO332_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5944279.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DAJTRQ010000218         3640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000218 DAJTRQ010000000
VERSION     DAJTRQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.222"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..223
                     /locus_tag="OO332_004043"
     CDS             <1..223
                     /locus_tag="OO332_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5944280.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            269..478
                     /gene="hha"
                     /locus_tag="OO332_004044"
     CDS             269..478
                     /gene="hha"
                     /locus_tag="OO332_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5944281.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            517..1107
                     /locus_tag="OO332_004045"
     CDS             517..1107
                     /locus_tag="OO332_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5944282.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1347..1607
                     /gene="repA"
                     /locus_tag="OO332_004046"
     CDS             1347..1607
                     /gene="repA"
                     /locus_tag="OO332_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5944283.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1831..1905
                     /gene="tap"
                     /locus_tag="OO332_004047"
     CDS             1831..1905
                     /gene="tap"
                     /locus_tag="OO332_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5944284.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1898..2755
                     /gene="repA"
                     /locus_tag="OO332_004048"
     CDS             1898..2755
                     /gene="repA"
                     /locus_tag="OO332_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5944285.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          980 a          888 c          910 g          862 t
ORIGIN      
        1 ttttcagcga gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa
       61 tattaatcag tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga
      121 tcccgtgcta cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga
      181 tgctgatccg gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat
      241 cattcatctg ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca
      301 gttacggcga tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag
      361 tttatcaaac agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt
      421 aatcaccgga aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata
      481 attcagcaat aattaattgc caggagcaga taaataatga agttgattat ttttattctc
      541 attgttctga ttattgcaac gctacttatc cggattatcc tcagaagtgt aaatcagcat
      601 tctcctttgc tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata
      661 ctgtccagcg gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt
      721 tttatgaaag gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta
      781 cgggtcgcgg atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg
      841 cagttattca ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg
      901 ttcagtattg ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga
      961 cgccgggata ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc
     1021 cacgatgcca gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat
     1081 cagcagtcac cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga
     1141 aacgggaaaa agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt
     1201 tcacagttta aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat
     1261 cttcgccaca attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat
     1321 ccctgtttga gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat
     1381 tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg
     1441 ccgctgttgc aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc
     1501 tgaaagatct catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg
     1561 agaaaatcat caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg
     1621 tggcgtagca tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag
     1681 gtggcaggga actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata
     1741 atcttcttta actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca
     1801 acaatcaagc tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg
     1861 tgctcgctcc ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta
     1921 ttaccgccag gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct
     1981 tccgttctgc gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc
     2041 cattcatgtg gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg
     2101 tcgccgggcc attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa
     2161 ccgcgtccag tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc
     2221 tgccggaaaa ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg
     2281 actgattacc taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat
     2341 cacgttcaca tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc
     2401 gcgccgcagt cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct
     2461 gggtatggat gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta
     2521 ccagacagag cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag
     2581 gcagcgtcag gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg
     2641 gcgcttcact gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg
     2701 catgattctg tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg
     2761 acctcctcag aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg
     2821 cacaaaaaat cagcgctaca tacaaaaaac aacctcatca tccaccttct ggtgcatccg
     2881 gtcccccctg tttttgatac aaaacacgcc tcacagacgg ggaattttgc ttatccacat
     2941 taaactgcaa gggacttcac cgtaaggtta caaccgttca cgtcataaag cgccagccgt
     3001 caaccctaca gggtgcaatg tatcttttaa acacctgtgt atatctcctt taaactactt
     3061 aattacattc atttaaaaag aaaacctgtt cactgcctgt cctgtggaca gacagatatg
     3121 cacctcccac cgcaagcggc gggccccaac cgaagccggt ttagttacaa catgcaaaca
     3181 taaaccccta aaaaacccaa gtccaacgca gaactgaaac cacaacgccc ctcctcataa
     3241 ctgaaaagcg ccgccgcccc gcccgaaggg cgggaacaga gtcgctttta attatgaatg
     3301 ttgtaactac atcatcatcg ctgtcagtct tctggctgga cgtatcgagt acacgctcgt
     3361 aagcggccct cacggcccgc taacgcggag atacgccccg acttcgggta aaaccctcgt
     3421 cgggaccact ccgaccgcgc acaaaagcat tttcatcgcc gatagcgggt atggcttggc
     3481 agggccggga tgggtatagg taatttggct atcgtaaaaa aggtggttaa aaattacaac
     3541 ctgcaatgta aattggctag ttaatttttg gctatttaaa ttgacttctt aacggcgttt
     3601 actgttccat tgctccccaa cccccctaat caggtctggg
//
LOCUS       DAJTRQ010000219         3616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000219 DAJTRQ010000000
VERSION     DAJTRQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..258)
                     /locus_tag="OO332_004049"
     CDS             complement(<1..258)
                     /locus_tag="OO332_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5944286.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVY"
     gene            1010..1558
                     /locus_tag="OO332_004050"
     CDS             1010..1558
                     /locus_tag="OO332_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5944287.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1121 a          682 c          810 g         1003 t
ORIGIN      
        1 atagactcct gccgctacgt acgggtcagc atcggcccag gcctgagctg cttccagcga
       61 ttcaaattca gcaataacgg ttgagccagt aaatcccgca gcccctggat cgttactgtc
      121 taccgctggc attggaccag ctgtcaacaa acgaccttca tcatgcagta actgtaaacg
      181 tgctaaatgt gccggacgaa cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc
      241 gtaaataaca tacaacacgg gcacaactcc ttgttcggga aagttgtaaa gtacgttatt
      301 tgaaagggcg aagatctgca acggaaagat gatgtctttg ttaaggtcat gcataaagta
      361 agggtaatta cgccaaaaat gacattttca ctgatcctga tcgtcttgcc ttattgaata
      421 tgattgctat ttgcatttaa aatcgaggcc tggtttttct actgaaatga ttatgacttc
      481 aataaccctt gaatgaagtc tattcggttt tctaggagac ctgttaaaaa caggacaata
      541 tcaggtgaat gactatgcaa tttaatagta gatttccaag tatctaagca gttttacaca
      601 aattctatgc agtatcggta tagtctcttt ttagcataac ttattgaaat atatagagaa
      661 tctgtgaagt ctcttttcag ggaactatgc agggtgttag tcgttcctct ctcgactcct
      721 agaaaagtgc tatatacagg actatataac gatatataga tgtttggtca catggtgtaa
      781 ctatcaagaa acttaacgat ttcctacgtg aaagttagaa agtgatggtt atagttaccc
      841 tcctaaaatc ttcatagctc cttcataacc agtcttaaaa ggacttcctt ttgagacact
      901 ttcaacgtaa cggtagaatg caccctcaag tagtctcaaa aggttgtttt tggattatga
      961 gacagctaaa cttatgagac taacttttga gacttctaag gggaacatta tggcggtgat
     1021 tggttattgc agggtatcaa cacaagacca gactgtagac aaccagaaac tacagataga
     1081 gaaggttcac aaggtcgata aatggtttat tgatgaggct gtctcaggag ctacaaaggc
     1141 caccagcaga gatggtttta gaaacatgat gaactatgta aggtgtgacg atacgttagt
     1201 tgtggtagct attgaccgtt tagggcgtaa taccgttgat gtgctctcta acgtggaggc
     1261 actacagact aaaggtgtga gggttgtttc tctacgtgaa ggttttgact tattaacacc
     1321 aatcggtaga gcaatgctta ctatgatggc tggtttagct gaccttgaaa aagaccttat
     1381 cagtgaacgt agaaaggctg gtattgaacg tgctaaagct gaaggggtac atatggggag
     1441 gcctgtaaaa gcttcttcag aggctgtaca gacgcttatt tcacaaggta agacgaggtt
     1501 gcagattcag gaagaattag gaatctctag agcaacttat tacagacttg ctaaataagt
     1561 tactgaatag ttacatggat gtagctgata tttggctttt ttgcaaaggg gatcacatag
     1621 ctggtgtaac aggtacttag cagctacaga catctgtcag atatttttga agattctcag
     1681 agagggaatt tgaagagggg ctactatgat tttataaaaa ttttcagagt ctgaaaagtt
     1741 actgtacaga ttttatacaa actaaaaagg actcccgtag gtgattcagt aatgaattac
     1801 ttcacgaaga gtccctttac aagtcgattt ttaatcgtgg ctacgacaat ccacccatac
     1861 cagacctaac cgtaccccac cttaccaagc cataccctac cccgccagac tataaggaag
     1921 cgtagagaac cgatgaaggt gtctgcacag ttgcatctta aacaaccatc acagtgatgt
     1981 caaatagttc ttttaagtag gaggggttcc cctatggtca catatcccct ctctcccgct
     2041 tagtgtccct tgatagtatc tagagagtat gcctgtaatg ttagagagta tgcctgtaat
     2101 gtttctgtat caggtcagct ataagaggtc tggatattac cttgtaagat tcttggtagt
     2161 gattatgcag agggcttgat aagagtgttt aacgggagga agagaaccat gaaaccgaag
     2221 gtaggttcta tcttaacagt gactgtaaga gatacctgaa aaaatacttt aacagaacta
     2281 ccaagaagag agggaacgga agagagggaa cgataagacc agaggtagtg acctgttaaa
     2341 caggctgttt caaagtctct taaaagtctg ttaagagggc taggtgaaca atcctgccat
     2401 tttgtcaagt gttttgtctt tagcaaaaag tgctaaactt aacaaaaagc actaaatgct
     2461 aaagaggcga ttaagaaata tactaagagg ggcgcttcgc ttggggtcac tatgttcttt
     2521 ctctcccgtt aagaggattt acagggacat ttcaaagccc tgataaggta agcccgatga
     2581 taacagaaac agagggcaaa accacttatc aacactgtga acgaataacg taaagggctg
     2641 gttaagaaag tcggtttagg gattggtaac aataccaatt agaagggagc ttccctttct
     2701 ggggtgtcgc tctatgggtg aagttaacct ttgaagttct ctgtgaagaa actgaacagc
     2761 tattatattg gctaattaca accctttgaa agataagcat ttttagaagt tactgttaag
     2821 gttgcagagt tgtctgtgaa gagacttgtc agttctctat attgggtggt tacaactcat
     2881 tgaaaaataa gagcttttag aggttactgt cagagaggtg aatacttgat agatacagtc
     2941 ttccggttct gtcaggctat tttttaacag atagcacaaa atgctaaaga tgcccctatg
     3001 ccatctcgta caggggggtt ttgaagggag tttaacgggg gtctgtgtgg tcattatcct
     3061 taaggtcaag cgtccatgtg cgaccgaaca gtttaaagac aacaagcact ctgacgagga
     3121 cgtaacgaag tagcagatct gcaataagtc caatacctcc accagcccat acatcaggtg
     3181 accccagctc aggagggaga tttaccatta gttttccttg taaggcgctc tcaatcgcat
     3241 tctgacacaa aaaagtacct gaccttatga gttgacctga agaggtcatt taactcgtta
     3301 cagagcgatt gaagaggtct gtgtgcaatg caataaccat gtgaccagat acatagccac
     3361 gcctcatatt ttactctccc cataaaccca ttacaccgca gatgttacga aattgttaaa
     3421 aatacttggt attagtcttt gaaaatgcta gcattgtata tataagttaa cagaataata
     3481 acatgttatg aggggcagaa tatgaatgag agacgcagaa tttagagcga tgcttcaggc
     3541 atcaagagaa cgcaacaagc acaactccta cgcttacaca aacaacccca ccagctacga
     3601 agtcccaacc tttagc
//
LOCUS       DAJTRQ010000220         3590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000220 DAJTRQ010000000
VERSION     DAJTRQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            29..244
                     /locus_tag="OO332_004051"
     CDS             29..244
                     /locus_tag="OO332_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5944288.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="OO332_004052"
     CDS             237..623
                     /locus_tag="OO332_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5944289.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="OO332_004053"
     CDS             631..1317
                     /locus_tag="OO332_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5944290.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OO332_004054"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="OO332_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5944291.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="OO332_004055"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="OO332_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5944292.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..>3590)
                     /locus_tag="OO332_004056"
     CDS             complement(3318..>3590)
                     /locus_tag="OO332_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944293.1"
                     /translation="IPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYD
                     DDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          986 a          663 c          783 g         1158 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat
//
LOCUS       DAJTRQ010000221         3586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000221 DAJTRQ010000000
VERSION     DAJTRQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1058..1498
                     /locus_tag="OO332_004057"
     CDS             1058..1498
                     /locus_tag="OO332_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944294.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1577..3274)
                     /gene="treA"
                     /locus_tag="OO332_004058"
     CDS             complement(1577..3274)
                     /gene="treA"
                     /locus_tag="OO332_004058"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5944295.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT          949 a          789 c          844 g         1004 t
ORIGIN      
        1 tcctgaaaag caaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat
       61 caaagtttag ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct
      121 ctatcattaa aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat
      181 catttaaata tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt
      241 acttgtttta caatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg
      301 tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa
      361 cctatgttgt tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt
      421 accgcaacaa gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga
      481 agatatttat tttcttagag agagtaattt actgaatgcg tttattcgaa cgtgcaaaaa
      541 aagagatgat aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta
      601 tgggaacaga attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg
      661 gtgtagcaat gataacattc tccattttat taatgcattt ctcattcggg tgaaatttcc
      721 gctacctgga cgcataaaac cggaactctc gccaaaagcc gggcactccg aaggaattga
      781 cgcgttgcgc tgctctgtaa cggcatttac cgcaagccat ttatttcgcc agcgacaatc
      841 cgcagagaac caaacgcatc aggaaacgac tgaacacaat aaatactgta ttgatcaaca
      901 tattatactg gcacctccag cacgcagtga ggatacactg tatcatcagc gaatcttatc
      961 actgcggagc tggttgaacg actttctgag cgaaataagt cggttcccgg taacaacaac
     1021 agaatccttt tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt
     1081 agacagtagc cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct
     1141 tattgaacat ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc
     1201 aatgcttcag ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct
     1261 ggagtgtctg gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag
     1321 tgaacaccgt aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa
     1381 atcgccgggt gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca
     1441 cgtatggcaa acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa
     1501 aaaaccatag atatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg
     1561 tcggagtaag cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg
     1621 ctgacttaac ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga
     1681 tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat
     1741 attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt
     1801 tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca
     1861 cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc
     1921 catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt
     1981 gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat
     2041 tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca
     2101 ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg atcccttttt
     2161 gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc gttatctcct gcagctttgc
     2221 tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga
     2281 tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc gagctgaaat
     2341 cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg
     2401 gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat
     2461 cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt
     2521 gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg
     2581 gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg
     2641 caaagaaggg cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac
     2701 cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac
     2761 cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac
     2821 gtccgcccgg cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt
     2881 tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc
     2941 cctctggcgg aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat
     3001 ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg
     3061 gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt
     3121 gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt
     3181 cttctgcctg caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca
     3241 ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga
     3301 ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg
     3361 aaatctcatt ctgcgaacgg tcaggcattt tagaaaaggg aattaaaggg ataaataacg
     3421 gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcgacat ttggttcgcg
     3481 attagcgaag aaaaatcatc ataaattggt aaaaaatata acaggtagtt aaaaaacatc
     3541 agtgctgggt aaattgccgg atgacatcag aacgatgcca tccgaa
//
LOCUS       DAJTRQ010000222         3564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000222 DAJTRQ010000000
VERSION     DAJTRQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(149..679)
                     /gene="cybB"
                     /locus_tag="OO332_004059"
     CDS             complement(149..679)
                     /gene="cybB"
                     /locus_tag="OO332_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5944296.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            868..1869
                     /gene="gap"
                     /locus_tag="OO332_004060"
     CDS             868..1869
                     /gene="gap"
                     /locus_tag="OO332_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5944297.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(1911..3350)
                     /gene="aldA"
                     /locus_tag="OO332_004061"
     CDS             complement(1911..3350)
                     /gene="aldA"
                     /locus_tag="OO332_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5944298.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
BASE COUNT          954 a          898 c          810 g          902 t
ORIGIN      
        1 attaaaaata agccagatgg acgtatgggc aaggattatg gttttatttg tcatacaaat
       61 aaatatgata ggcgcttccg atgtagaccc gtattcttcg cctgtaccac gggtcggttt
      121 tagtacaggc gttttcttta aatatccttc aggaacgttt acgcggcatc atgcgtagaa
      181 gtgtgttgtc cttccagaaa tagtggtgtg ccagtgcagc cgcagcgtgc aacccgatga
      241 caaaatatcc cagattcgcc agcgtctcat gccacgactt taagctatca acccgttcga
      301 aattggcctc tgaagcgtaa ggcatcgtca aaccaaacgc aaaccacggg ttgccccggt
      361 tatacatcat caccaaacca atcactggca gcgcaataaa caggagataa atcaccaaat
      421 gtcccaaatg cgccagtccc gtcatcatcg gtttcggctt aggtataatc ggcggggttg
      481 ggtatttcag tcttaacaaa agacgaacca ccatcagcac gagaattgag atgccacagg
      541 aaacatgaat catgttgatg agcggccgat cgctacgtgg gaagaaacca cgaaactcca
      601 ttgcgcaata cgctgcgata accagcaaaa agaccagcca gtgaatgctg atttgtaacc
      661 ttgagtattt attttccata acatttcctg ctttaacata attttccgtt aacataacgg
      721 gcttttctca aaatttcatt aaatattgtt cacccgtttt cagtacgtta cgtttcactg
      781 taagaatgga gattgcccat cccacaatcc tggtctaagc ctggaaagga tcaattttca
      841 tccgaacgtt cctgacagga gaaaaccatg agtaaagttg gtattaacgg ttttggtcgt
      901 atcggtcgac tggtgttgcg tcgtttactt gaagtcaaaa gcaacataga cgttgtcgct
      961 attaatgatc ttacttcccc aaaaattctc gcctatctgc tgaaacatga ttcaaactat
     1021 ggtccgttcc cctggagcgt tgattttacg gaagattcac ttatcgttga tgggaaaagt
     1081 atcgcggttt acgccgaaaa agaggctaaa aatattccgt ggaaagcgaa aggggcagaa
     1141 atcattgtcg aatgtactgg cttttatacc tccgccgata aatcgcaggc gcatcttgat
     1201 gctggtgcga agaaggtgtt gatttccgct cctgccggtg aaatgaaaac catcgtttat
     1261 aacgttaatg acaacactct ggatggcaac gacaccattg tttccgtggc gtcatgcacc
     1321 actaactgcc ttgcgccgat ggccaaagcc ttgcatgaca gtttcgggat agaagtcggc
     1381 acgatgacga ccattcatgc ctatactggc acccagtcac tggtggatgg cccgcgtggt
     1441 aaagatttac gtgcttcacg cgcagcggca gaaaatatca ttccccacac tacgggggcg
     1501 gcaaaagcca ttggtctggt gatcccggaa ctgagcggca aactgaaagg tcatgcgcaa
     1561 cgcgtgccgg tgaaaacagg ttcggtcact gagctggtgt ccattctcgg aaaaaaagtg
     1621 actgccgaag aggtgaataa cgcacttaaa caagcaacca ccaataacga gtcatttggt
     1681 tataccgatg aagaaatagt ctcttccgat atcattggca gccatttcgg ttcagtgttt
     1741 gatgccacgc aaacggaaat taccgccgtg ggcgatttac aactggtgaa aacggtcgcc
     1801 tggtacgata acgaatatgg cttcgtcatg cagcttattc gcaccctcga aaaattcgct
     1861 aaactctgac gcgcacaggc ggaggaaaaa acctccgcct ctttcactca ttaagactgt
     1921 aaataaacca cctgggtctg cagatattca tgcaggccat gtttaccatc tgcgccgcca
     1981 ataccggatt tacgccatcc ggcgtggaaa ccttgcatag cttcgaagtt ttcacggttg
     2041 atgtaagttt caccaaactt cagcccttta atggctttca tcgcgacgtt cagattttgg
     2101 gtatagattg atgaggtcag gccgtaatca ctgtcattag ccattgagat agcatcttcc
     2161 agcgtgtcaa atgcgacaac tggcagcacc gggccaaagg tttcctcatg cataatcgac
     2221 atttcctggc gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc
     2281 gctttgccac ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctca
     2341 accctttcca gcgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg
     2401 gggttaccaa attgaaccgc ctgcaacgct tcacccagcc gattgacgaa ctgatcataa
     2461 atgcctttct gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg
     2521 cgtgaatcaa cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagcc
     2581 ggtgctttac cacccaattc cagacacact ttggtgatgt ttttcgccgc agtcgccatg
     2641 atcttctcac ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc
     2701 gccagttctt gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga
     2761 aggcctattt catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg
     2821 ggtttaatga cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg
     2881 aagaacggaa agttccacgg cagaatgccg gtagtcacac caagcgcacg tttaaacaga
     2941 agaatatttt ctcctggacg atcgctttga ataatctcgc cctcgtaacg ccgtgcccac
     3001 tccgccatgt aatcgatata gtcggcagta aaagccactt cgacttcagc tagctgctgg
     3061 atcttgcccc cttcttcaac aatcagcgca ctgatttcac tggcgcgttc gcggatcccg
     3121 gcggagattt tgcacaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt
     3181 tgtgcacgtt ctgctgcatc gattgcctta cgggcatcct cggcctgacc atcgggtatg
     3241 cgggaaatga cagcctctgt agcagggttt accacatcaa tccatgcgtc tccacgccag
     3301 gtaacaaact gtccatcgat atacatagga tgttgaacgg gtactgacat gggcgactcc
     3361 tgtgatttat atgtttgttt tcggtgaata tattgttaat taacatcaat catcagagag
     3421 acggcatcgg gaaaggacaa ttctgtggag ggcttcataa aaacaagtgt aaaaggaata
     3481 aaaggcagaa ataaaagtca ggagtcatgc ccgtttgcaa acaagtggtt cacaaacggg
     3541 caatttttaa cgtaacgagc gact
//
LOCUS       DAJTRQ010000223         3514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000223 DAJTRQ010000000
VERSION     DAJTRQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            289..807
                     /locus_tag="OO332_004062"
     CDS             289..807
                     /locus_tag="OO332_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5944299.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="OO332_004063"
     CDS             814..1047
                     /locus_tag="OO332_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5944300.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="OO332_004064"
     CDS             complement(1349..1816)
                     /locus_tag="OO332_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944301.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2886..3077)
                     /locus_tag="OO332_004065"
     CDS             complement(2886..3077)
                     /locus_tag="OO332_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5944302.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3447
                     /locus_tag="OO332_004066"
                     /pseudo
     CDS             3142..3447
                     /locus_tag="OO332_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          844 a          787 c         1042 g          841 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgc
//
LOCUS       DAJTRQ010000224         3387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000224 DAJTRQ010000000
VERSION     DAJTRQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..934)
                     /locus_tag="OO332_004067"
     CDS             complement(<1..934)
                     /locus_tag="OO332_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HCR5944303.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIANILM"
     gene            complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="OO332_004068"
     CDS             complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="OO332_004068"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5944304.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2188..2970
                     /locus_tag="OO332_004069"
     CDS             2188..2970
                     /locus_tag="OO332_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5944305.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2972..3198)
                     /locus_tag="OO332_004070"
                     /pseudo
     CDS             complement(2972..3198)
                     /locus_tag="OO332_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         1004 a          773 c          672 g          938 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagactatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt gggaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaataccag acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aaagtgtaca accaccaata tacagcgtca
      661 ggccaattaa aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg
      721 caattaaaaa ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta
      781 gcgaaaatac acttttgaat tgagtgatta atatgtcatt taatgcagct gcgcatcccc
      841 atagcgggaa taaacacgat aacaaaataa actggaacaa gggagtctta ttcagatacc
      901 catcaggcat ctgaatgatg tttttatcgt tcatagtgct acctttaact gtgcaggatg
      961 attattcgtt taaggttaaa aattcattaa attgttcaat actcggataa gatgattgca
     1021 tacctttccc tgtgacgctg aaagcggcaa agagagcggc ttttttcaaa gcggcttcaa
     1081 catcaccgct ttgaacataa taatgggaaa agcaaccaat aaatgcgtca ccagcgccac
     1141 tagtatcaac agcatttact ttgaatgcag gaacatggac ttcctgatcg cgggtcatcc
     1201 ataatgcgcc tttttcgctc atggtaacaa taatattgtt cagcccttta tcaactaacg
     1261 aacgtgcggc caaacgaata tgatcataag tatcaaccga cataccagtt aatatttcca
     1321 gttctgtttc attcgggata aagaaatcac ctttgcaggc ataagacata tctaactcac
     1381 gcaatgccgg agccggattt aataacactt caataccatt tttcttacca aactcaatcg
     1441 cgtggtaaac tgtttccagt tgaacttcca gttgtaaaac gatcaatttg cattttttca
     1501 gatcttctgc agctcgatcg atatcttccg gggaaagaaa tttattcgct cccttaatta
     1561 ttaatatact attgctcgag ttggcattaa caaagatcgg tgcaacacca ctgctggtac
     1621 aggggacctt ctcaacataa gtggtattaa ttccccatga ttcaagatta cgaatagtat
     1681 tatccgcaaa aatatcatca cctactttag tcagcatcag gacttttgaa ttcaatttag
     1741 ccgccgccac cgcttgatta gcacctttcc caccacatcc gattttgaag gcaggtgctt
     1801 ccagagtttc tccttcttta ggcatctgat tagtgtaagt aatgagatcc accatattgg
     1861 aaccaataac tgcaatgtcc atttcactac ctcttataaa ctttcgcata acaatggtat
     1921 ttaaataaca ttagcatgtt acttttgcat catttgtgac tgagatcgcg attagcacat
     1981 caacccgatg tttatttaat agacttccag tctcatcact caggccaaca ctatctaatc
     2041 ataagcaacc taacaagatt agtgcccaaa attcagcagc ctataccctt ttcatttcaa
     2101 aggggcggtc gtatagtatg gtaatgaaaa caatgtttac taacgccaaa atgttatttt
     2161 tataacattc ttacggagag agagttgatg gaaacgaagc aaaaagagcg tatccgacgt
     2221 ttgatggaac tgcttaagaa aaccgacaga atccatttga aagacgcagc gcgaatgctg
     2281 gaagtttctg taatgactat tcgtcgcgat ctccatcagg aagatgaacc tctgccactg
     2341 accctactgg gtggctatat tgtaatggtg aataaacccg cgctatccat gccagtaatc
     2401 catgacgttc caaaaaatca tcgtgatgac ttacctattg caattctggc tgccggaatg
     2461 gttaatgaaa atgatctgat cttctttgat aatggccagg agataccact cgttataagc
     2521 atgatcccgg atgcaatcac cttcaccggc atctgttact cacatcgcgt ctttgttgcg
     2581 ttgaatgaaa agcctaatgt aacagcaata ctttgtggtg gtacgtatcg tgccagaagt
     2641 gatgcttttt acgatgccag taactcttcg ccattagact ctctcaatcc gcgaaaaata
     2701 tttatttccg ccagcggtgt gcataatcac tttggcgtca gctggtttaa ccctgaagat
     2761 cttgccacta agcgtaaagc gatgaaccgt ggactacgga aaattttgct cgcccgccac
     2821 gcgttgttcg atgaagtggc ctctgccagc ctcgcaccga tctctgcatt tgacgttctg
     2881 attagcgatc gtccgttacc ggcagattat gttacgcact gccagaatgg ttctgtaaag
     2941 atcattacac ctgattcaga agacgaatga cttactgaaa aaacaccaca atcttgttaa
     3001 acatcgtcgg gttggactga ttacgttgca ctttcaccac atattccagc ttatctattt
     3061 ggcttatcac ctactccaga cgctggtcat ccttgaccag tagccagata tggcttttgt
     3121 cgcagtcctg aatcggcagg cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag
     3181 cccacaaagg tgagtcatta cacccggatg gtaaacaggc acttccttgt tgtagaagcc
     3241 gttatattgt ccataatgtc tgggttttta atgcatactg ggtaacaacc ggacgaatct
     3301 tcgaatatca ctggcgctgc cagccggttt gaattcatct cacaaccctg catagagcga
     3361 atctcttgcc tgcacgtcac tctgaat
//
LOCUS       DAJTRQ010000225         3352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000225 DAJTRQ010000000
VERSION     DAJTRQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(404..601)
                     /locus_tag="OO332_004071"
     CDS             complement(404..601)
                     /locus_tag="OO332_004071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944306.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            1447..2496
                     /locus_tag="OO332_004072"
     CDS             1447..2496
                     /locus_tag="OO332_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5944307.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            complement(3269..>3352)
                     /locus_tag="OO332_004073"
     CDS             complement(3269..>3352)
                     /locus_tag="OO332_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944308.1"
                     /translation="LLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT         1089 a          599 c          564 g         1100 t
ORIGIN      
        1 tatcatcatt caatgaacag atagctgtag tcaatgtatt attatgcacc acactgttaa
       61 ttttttgcaa aacaaacaat tacaatacaa aaccattatt aaatgcgatg gggatttcac
      121 tattagttat tatttatatt gcaacttgcc caggaaataa agtcaggtat tatgcagaga
      181 tagaacattg gtttaaagag tatggtaatt ttaatataat ccaaagatct atgcttggac
      241 ttaacttata tgcagatatg ttattttctg taaaatcaat tatcccagca ttactagctt
      301 tttccagtac tattatatgc aagaaaaaaa caaaaatact ccctctaatt tcttgctgta
      361 tcttgatcac actatatatt atacatacac cgccagttat ttttcaagcc atacattttt
      421 ctgaaagcaa tcttttctcg acactctccg tgtttagggt gtcttttgcc atgatactta
      481 cagcactcat tatatttcca accgtaatat cacttaattt taatgtcact tcaattttca
      541 tatccacaat gattattgga accattgcca caacatctat gttaggatta tcaccaagca
      601 tatatgcttc tggtaataga atttatttta ttccatattt gcttcttata acagcaattg
      661 ttgtttcaac cccaatcgca ataaataaca tagtagcaaa ttttgccaag agccattata
      721 aaatttgaat taaatttaaa agccacctcc tttttttggg gggggcttag aaataaaaac
      781 aaatttaatt ggacttttat tggttataac acattcctct atactgctca tcacccgact
      841 atactaggat tttcgtcatt ttttgctgaa ccgctctcat gaaatcctga caacaaagtc
      901 atgtttcacg tcaggatact tcttctggca tggaaatata ccgtgtgcag ctgatgcata
      961 tttatatttc aatataatac gatattgaat ggcctacacc actggtagtt caggccaaat
     1021 gacatccgac ggtttcacga tgaactacgg caaaagaata tcagagccct cattcctccc
     1081 cgaaagggag cgggctctgg tctggtgagt atgcagatcg taaaggctat ggccatggtg
     1141 cgagagtggc agctatgcca gaaagagtgt gtattgcctg agactctaac ctgatgcaga
     1201 gacgattgtc ttgaacctga tttattcaac aaagccgctg gcaggatgtt acgaaagtta
     1261 tcgcggccgc aggctgattt gatatcgata ctatcggtgg tactgcggaa gtaaatgcct
     1321 atatagtgcg actttgaaaa ataataaatt aatattgaac tcgttgatta ataatcataa
     1381 actttcaggc attcatattt gcaatgagtg tttgaaaatc acacaaaact ggagagccta
     1441 ctttaaatgt ataataacaa aatcgacaac aacagaaaaa caaggcatga ttgggttgat
     1501 gctttaaaat ttctcggtat ttttgctata tatttaggtc accttggttt gggggctggc
     1561 aaattgtatc cttttgtttt tagttatcac gtcccactat tctttttcgc tgctggtttt
     1621 tttactatca aaaaaaatga cttatctatt ttcgattata tcaagtcaaa gttttacaga
     1681 ttaatgattc catatttcac ttttgcattt actatattaa taataaacac aattaactca
     1741 ggtgagacga ttgattatat atatagccac atttatgata taatatatgg tgttagaaac
     1801 aaccaatttg taggaacaat atggtttata aactgccttt ttgttataat agcaattgat
     1861 gcaatcttta aagaaatagt caagaacaat atagtgattt taatcatatc actattatcg
     1921 ttcatgttgt cacaaacagt gctcaatcat aacccattac tagatcctca atggttttgg
     1981 aacatagact ccgctatggc atactggtgg ttattgcctc tcggtagatg tatgttttta
     2041 gaattaacac gtgacaggtt ttttggaaaa tctaaaattg gtttcattgt cttctcaatt
     2101 accgcgataa tgagtgcgta tcaactcctt aaccagaaac ctttgctttt taagattatc
     2161 tcaatattca atgcagacat aatatcaagt agttatattc aagctattaa tactattatc
     2221 accacggtag gcttgataat atttaatatt tttatagcaa aaataatttg tgggaatgac
     2281 tttattgtaa gagctggaag aaatactctt aacatatgtg gaatggagta tatcaccaaa
     2341 ttatttatcc ctatggcttt agcaatcata ggcttcagcg tgaccattcc aaatcctatc
     2401 tgcgcaatca tatatacctg tatttgtgta tatgtatcag ataaaattgg ccattggcta
     2461 tcgaggacag tcggtgggcc atttttgata aaatgataaa aaggcgcaat taattgcgcc
     2521 tttttataga tatatagcgc atatttatgc tcagattata ctgttaaaat taatgcgcaa
     2581 atgttacggt tttctttcct agcaactgaa gggaatgttc gaagtgcaaa cataaaatta
     2641 ccccttcact gaacagatat tcagtcggcc aacacatgag aaagaaatac accagatgaa
     2701 ttaaaatatc atatacttat taatcaaaga aataaaagca taaacagtag gtggtgacgc
     2761 tgataaactg gctataaaca acctggaagg tgatgagcta tcgcaatata atttattggt
     2821 gaattatttg aagccgattg ataacaccag tgctcaaaag actccccccg gtaaccataa
     2881 gtagctaata tctagtacag tttcgatatc tattatcgtt actgcgtcga tatagtctaa
     2941 atagctgcac ctaattctgt cacacttgtc gtttacagat gggtaatgtg ttgcgtatgg
     3001 gcaacaaaac tcaagccaga tataacacaa cctagctgag aacgccttcc tgatgcttcg
     3061 cccagcaagg gctgagcgaa agtggagcac ttttctgcgc agctatttag gacagtattg
     3121 agtcatattg tttatgtatg agttaagttt cgtaatatca attaaataaa ataagttgaa
     3181 atcattgtag aagtgagcat tatacccagg ctacctgaac ctactgaaaa atgagtatta
     3241 ttccaacaat tatcctagat taacaagtct atttatttgg atgcataggc cactcgggtt
     3301 ttactacatt aacacgcata agcagcaccc gatatttttt ccatgccagt aa
//
LOCUS       DAJTRQ010000226         3199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000226 DAJTRQ010000000
VERSION     DAJTRQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.230"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="OO332_004074"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="OO332_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5944309.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPD"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="OO332_004075"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="OO332_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5944310.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3199
                     /gene="cusC"
                     /locus_tag="OO332_004076"
     CDS             2335..>3199
                     /gene="cusC"
                     /locus_tag="OO332_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5944311.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEH"
BASE COUNT          778 a          833 c          841 g          747 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaatcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacg
//
LOCUS       DAJTRQ010000227         3176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000227 DAJTRQ010000000
VERSION     DAJTRQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            846..1040
                     /locus_tag="OO332_004077"
     CDS             846..1040
                     /locus_tag="OO332_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5944312.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            complement(2065..3174)
                     /gene="fepE"
                     /locus_tag="OO332_004078"
     CDS             complement(2065..3174)
                     /gene="fepE"
                     /locus_tag="OO332_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5944313.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          941 a          781 c          657 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagata tttaacataa tatcttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccctaatata agatgatgtt tttttatgaa
      421 aatagtcagc ccccccccta aaaagcggtg ttggcgcagc tacaacaccg cgccgacacc
      481 gcttttttaa atatcacaaa gagagcaaga agaactaatt tgtcataact ctctatttat
      541 aaagaaaaat cagcaaaaac ttgtttttgc gtggggtgtg ggcatttcag gcatgagaac
      601 agactcgcgt ttttccggag tatcagcgga attattgatt aaggaacact cgacgcagag
      661 cgaaatttta tgcaacagcg gctgcactgc acagggatta tttatcagtt gatatgctgt
      721 gccatgatac gtcctaaaga aacaggacag gatgtgcaat tgtaataccg tcgcccgcaa
      781 cgtcttacaa ttaccacctg ctcagccccc ccccgacacc cggaactgac ggagaacaac
      841 ggaaggtgaa caaacaacaa caaactgcgc tgaatatggc gcgatgtatc aaaagccaga
      901 gcctgacact gcttgaaaaa ctggatgctc tggatgccga cgaacaggcg gccatgtgca
      961 aacgactgca cgaactcgca gaagaactcc agaacagcat ccagattcgc tttgaggccg
     1021 aaagtgaaat aggaacataa cgaagctgac ggaggcggtc acggctttcc cgtgggcgtt
     1081 ttcgtcagca gacaagcccg tcagggcgcg tcagggggtt ttagcaggtg tcggggcgca
     1141 gccatgaccc agtcacgtag cgatagcgga gtatatactg gcttaatcat gcggcggcag
     1201 agcaaatggt gagagaacag cgacatatga ggtgtgaaat gctgcacaga tacgtaagga
     1261 gaaaatgcag cttccggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1321 ttcggctgcg gcgagcggtg tctgctcacg caaagacgga tatacggtta tccacagaat
     1381 caggggataa caccagaaaa aacatgtgag caaaaaagag gccccgaaaa aaggccgcgc
     1441 cggtggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaac cgacgctcaa
     1501 gtcagaggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtggctc
     1561 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     1621 gcgttcgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     1681 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1741 ttgagtccaa cccggtaaga cacgacttaa cgccattggc agcggccact ggtaaacagg
     1801 atgtgcgaga gatgtattga gagttcttga agtggcagcc ttactgaggc tacactagaa
     1861 ggacagattt ggtgacatgt ctcgcacaag actgttacca cggttcagaa gttccccaac
     1921 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcta gagtagaaga
     1981 ctacggctgt aagggaatga tctcaagaag atcctttgaa ctttttataa aagttgctta
     2041 taaaatgatg catcagcaca acttttactc aatcacactc atctttctgg cctccatggc
     2101 atgacgtaaa agtatatagc cacatgcaag catccctcca atgacagaag aaagaattac
     2161 aattatggca ttacttggcc catcctttct gaccggtaac gttgggctca attgatactg
     2221 aaatggcata aaatcaacat cccccacttt cgccacaacc aattgattca caacatattg
     2281 acgatttcgc aactccccac tcaattccga aacatccttg attgattttt tgatgttcaa
     2341 tttagttgct ataccatcag aaccgagagc cacaggaaaa tctgggtcat ccttcataat
     2401 ctgaccattg ctgtatacag gtttttttat tccagccgca ttagcaacct ccagtgaata
     2461 attgaggcgt cgaatatctg cattcagctg gtttctaatt tttatctcat ccagtgcaag
     2521 aatttctttt tcaacattag ttttaacttc tagtttattt cttatatctt ccatcaaatc
     2581 ccttacaaca agggaagaaa cataattaat atacccagac aacacatcgt gagcttcttt
     2641 acttgttgga gcagtaaaac tcagagtcca tgatacatac aatgatgact tgtcgttttt
     2701 cttactggca ttatcatcaa tcgacttcat atttttactc aactcaacaa ttgcacgatg
     2761 cagttcgaga gcgctaatgt tattcttttt aataatctcc ataacataag gagatgatct
     2821 catatactca ttcaacgaat taacggactg aaatttctta ataaacagag caaatgcgct
     2881 atcacgattg atatcaaaat ccactcccag aacatgaagc ttggaaagct ccttatttaa
     2941 ttcactccat tgaacagact cagcaggagt aaccacagca gaactagtcc atttttgtgg
     3001 caaaacaaaa gaaacaagaa agccaataca agcaaaaaat agaatcaccc caacaatttt
     3061 tatttttgct gcccataaaa cgctaatcaa gccaaaaagg tcaatttctt cattaccatt
     3121 cggcaacatg ttgtaattag aaaaatgttc gtcagagacc tttgagattt tcattg
//
LOCUS       DAJTRQ010000228         3142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000228 DAJTRQ010000000
VERSION     DAJTRQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO332_004079"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OO332_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5944314.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="OO332_004080"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="OO332_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO332_004081"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OO332_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5944315.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO332_004082"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OO332_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5944316.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="OO332_004083"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="OO332_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          762 g          837 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DAJTRQ010000229         3109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000229 DAJTRQ010000000
VERSION     DAJTRQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..72
                     /gene="ldrD"
                     /locus_tag="OO332_004084"
     CDS             <1..72
                     /gene="ldrD"
                     /locus_tag="OO332_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5944317.1"
                     /translation="DLAAPVIAGILASMIVNWLNKRK"
     gene            complement(159..1838)
                     /gene="bcsG"
                     /locus_tag="OO332_004085"
     CDS             complement(159..1838)
                     /gene="bcsG"
                     /locus_tag="OO332_004085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5944318.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1835..2026)
                     /gene="bcsF"
                     /locus_tag="OO332_004086"
     CDS             complement(1835..2026)
                     /gene="bcsF"
                     /locus_tag="OO332_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5944319.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2023..3109)
                     /gene="bcsE"
                     /locus_tag="OO332_004087"
                     /pseudo
     CDS             complement(2023..3109)
                     /gene="bcsE"
                     /locus_tag="OO332_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          775 a          798 c          822 g          714 t
ORIGIN      
        1 gatttagcgg ctccggtcat tgctggcatt cttgccagta tgatcgtgaa ctggctgaat
       61 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
      121 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      181 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      241 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      301 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      361 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      421 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      481 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      541 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      601 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      661 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      721 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      781 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      841 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      901 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      961 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
     1021 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1081 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1141 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1201 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1261 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1321 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1381 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1441 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1501 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1561 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1621 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1681 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1741 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1801 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1861 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1921 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1981 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     2041 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2101 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattccac attgttctgg
     2161 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2221 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2281 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2341 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2401 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2461 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2521 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2581 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2641 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2701 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2761 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2821 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2881 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2941 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     3001 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3061 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DAJTRQ010000230         3082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000230 DAJTRQ010000000
VERSION     DAJTRQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(143..871)
                     /locus_tag="OO332_004088"
     CDS             complement(143..871)
                     /locus_tag="OO332_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5944320.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(1834..2487)
                     /locus_tag="OO332_004089"
     CDS             complement(1834..2487)
                     /locus_tag="OO332_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR5944321.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(2480..>3082)
                     /locus_tag="OO332_004090"
     CDS             complement(2480..>3082)
                     /locus_tag="OO332_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5944322.1"
                     /translation="AKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAM
                     VFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT          746 a          786 c          755 g          795 t
ORIGIN      
        1 ggctattagc tcgtctcggg attggtgctg gcgggtctca tcttccgggt ctagaccaag
       61 ctctctgagg aggacttcag tccggtagcc acggtaacgg agagtcatgc gcccctcagg
      121 tgtgcgcggg tcaatcatca tcttagattc cctccacctt accggattgt gccagcttca
      181 ggaggtgctc ttgcttctgc tggtcggtgc cagtgaatgc aataaggatg cctcgcatct
      241 tgtcaaccat ctcaggctta ccgaagacct ccagcccctt agcgttgaac cggaagttat
      301 acccacctgc tgagaacttc tggtctgacg gcttaatgat acgaaagcag tcggaatact
      361 tcggatttac agaccactcg ttactgattg ggttcttacg aaagaggcca cgatgctgga
      421 gaagtgcggt cattagcttc tgagcgggca gaccataggt tttgcagtag tcgccccaag
      481 tcacgcctcg gcggttagcg gactgaatgg ctgctcgctt cttctggtct tccagttgaa
      541 ggactcgacg ctctaacgtc tgaatatggt cgtaacactt ggcgcggagg tgaacggaat
      601 agccagcgac cagaatgaaa gtatgcttac ggtcgagacg atacagcggc tgctgtttcc
      661 cctgcgcgtt tagatagaca tcttcagtca ccccgatatg actgctcagt tttgagcact
      721 gctcagtttt gagcgcgtta agctcctcag ccatattgcg aatatcacgc aggacatttc
      781 ggtgctcctt gcctgtcagc tcagcaattt caactgaggt catggagaca agctggtcga
      841 ggttaatgac gctgatttct tgttgggaca tttacgtgta ctccttttgt ttgcaccctg
      901 aagtcgcacc ctactgacct actcaagccc ccacgtaaag ggagaccagc aggcaacagg
      961 acgcaaacag aatgacttga ggtaattgtg atgtggtctg cgatgaccag tagtgataaa
     1021 ggtgcaagac agcatgtccg acgtccagat ggtgagcggc gatagaccca agtcaaaatg
     1081 aagacacgag aacgctaacg ggctgggagt ctgacagagg ctgtcttgct agccttggta
     1141 tggagaaaga cttaatgtcg gggggcttcc gcataacttg acgcatcaag accagaggcg
     1201 gaatgtatgg cccctatagt gatagctaag ggtccattcc ctatagtgag ggtataagat
     1261 ttgctacctg ataaggccat aatctgacct ccagttagac ccgaagtatg gagaccacat
     1321 catagcgacc atagtttact tcgggatgac aggaggctgg acttaaagtt cttcttattg
     1381 gaattgtgat aaacacaatc cataacaaga taaactcatg gtctcctcca gcctgctggt
     1441 caggactcaa ggttcaacct cagacaccac tggtctaacc tccttgagtt tccgcctgtc
     1501 cacaatggat ggcagacctc agcaatgatg tcttggttct tcacaatctc gcacaccgat
     1561 ggtctcatag acggaagttc cgctgaggtc tgatgttctc cgcctgtcaa aggagagaga
     1621 ttgctacgtt aaagggaatg gtctcaggcc aaggtgattt aacactaggc gtctgcggat
     1681 ggtttccaat cggtccgcta ccggaagcct gagacttaat gaatgtgtgt gggtcgtgaa
     1741 tgttattaag gtggggactt agggtctctc ccctatagtg atagttaagc ccccttccct
     1801 atagtgaggg tatattcaat ctgacctaaa gctttactcg ctagctgcct gctgctgttc
     1861 cctcgccttc ctgtcccggt cctcaatgcc cttgagcgcc ctttccggca tgctgttaaa
     1921 catggcttcg acttctttcg gtctggtaat ctgctcagtg acaataatgt tcagcagctt
     1981 aaaaagacca tgcgctaact gagggtcgtc atcaatatta atttcacccg gatggaccgc
     2041 ctgattgcca acgatacgac aaatatcagc agctcgctgg accaatggag acaatccttt
     2101 ggctacaagc gacttaatgt ccgtgtcgat atgcttcccc ggctcaccga gatggaccat
     2161 cagcttctga agacttaagc gcaggagtgc ggcagcgcct ttaggagaaa ggttaacaat
     2221 gcttctggcc tccatgtagt cggacttaca gtcgtctggc atgtctgggt taggcatctc
     2281 aacgtttgct gacgctggta taatcatggt ttcgtcatac caataagctt tctcattgca
     2341 tcgttcacat ttagaaaagg cgaaaggtgt ctcataaatc ccgttagcgc catattttaa
     2401 gcggacccag cgctgttgag aataagcgcc acaattcggg cagttaaaag cagtgccacc
     2461 aaactctgcc ggataatact cagccattta ctccagtctc cttataaaca cccgccatca
     2521 cattaaccaa tctatctacc tcgacctctt tcgcgtaacg gtcgtaagtt atagacccag
     2581 agctatgacc cataatcgcc tgagcatacg ccaattcgac cccatgttgt tttagctggg
     2641 tcgcaactgt atgtctaaac gagtgaaaag ctaagccgct acggtcagca aggactttag
     2701 gtagcaacgt tcggttaaac catttgctca cctgctcacc gtagccgttc ttcatcagct
     2761 tgaggccatc gaataccata gcattatcac cctcaatctt tctacggtct tctacgagtg
     2821 atataaagtc agccaataca aaaccataag caccatcgac cagcgggaca cagcggtcac
     2881 tatgggcgtt cttgatactc ttacccggca ggctgctatc gtcttcgttg atatggataa
     2941 acacggtccc ggcctcagtg gtcctgacat ctttgacctg aagttgggcc acctcgttaa
     3001 gtcttgctcc agtgattgca gccagagcgg tgacgtaata atggtatggc ttacctgcgt
     3061 tcttctgaga gtacgcttta gc
//
LOCUS       DAJTRQ010000231         3069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000231 DAJTRQ010000000
VERSION     DAJTRQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.235"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(80..550)
                     /locus_tag="OO332_004091"
     CDS             complement(80..550)
                     /locus_tag="OO332_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5944323.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(597..1316)
                     /gene="yehT"
                     /locus_tag="OO332_004092"
     CDS             complement(597..1316)
                     /gene="yehT"
                     /locus_tag="OO332_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5944324.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1313..2998)
                     /gene="btsS"
                     /locus_tag="OO332_004093"
     CDS             complement(1313..2998)
                     /gene="btsS"
                     /locus_tag="OO332_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5944325.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          777 a          818 c          712 g          762 t
ORIGIN      
        1 ctggtgatga aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg
       61 catggccgcc atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca
      121 gaaaataacg taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca
      181 tcatcgctgt aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atagccagaa
      241 tgtcatcggt tttcagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa
      301 tgcgacgttc cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca
      361 ggccattgag gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt
      421 ctttacgtag ccagacggcg atctgttccg cggtggcttc gacattatcc agcgccagaa
      481 tacgcaccag gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt
      541 tacttatcat gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac
      601 aggccaatcg cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca
      661 ttacgcaaaa tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg
      721 aggttaacca gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt
      781 aatgtcaatt cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc
      841 cgactgctga caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta
      901 caagggataa atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg
      961 cgctcctgac gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc
     1021 agataatcaa aggcatgttc ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga
     1081 aaaacaatat acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg
     1141 cgcggcatct ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc
     1201 ccttccacgg cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat
     1261 atacgcaggt tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct
     1321 cgtccctcca tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc
     1381 ttattccata gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca
     1441 gcccactggc attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca
     1501 aatgttgccc ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg
     1561 tcccatgttt aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct
     1621 gggataattc ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt
     1681 caatttgcag ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag
     1741 gccgctttaa gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt
     1801 cgctgtcgcg gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgaggat
     1861 tcacctgggc gtgaagcagt ttgatttctg actgggtgag catcgctttt tgccgctcat
     1921 attgcccggc aagaatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt
     1981 ttattgaact gaataaacgg tttttggctt catacaattt gatggtgccc atcacgcgct
     2041 gattttcgcc acgcaacgga attaccagag tcgaccccag tttgcattgc ggatgcaaag
     2101 agcaacgata aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt
     2161 ttaaggtgta agtcgaagaa atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg
     2221 taaaggccag caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat
     2281 acagcacctg agccactttc atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt
     2341 ccgtcgaggc tgccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca
     2401 tcgcgcgttt atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca
     2461 tcattggcgc agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg
     2521 caaggatgat cagcatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg
     2581 ggttaaagac tttatcagtg cgcccgcgac ggatcaggat gctgtgtacc aggccgccga
     2641 gcaatccttc aacgatggtt gagatcatgc aacttagcgc ggtcatgccc cccatcgaat
     2701 atcgatgtaa gccgccggtc agaccaacca gcccaccgac gaccggaccg ccgagtaagc
     2761 cgcccattac cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa
     2821 accaggtgcc catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca
     2881 gacgaaccgt gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa
     2941 tgactaaaaa aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac
     3001 ccgcaaacca cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt
     3061 gaactcttg
//
LOCUS       DAJTRQ010000232         3006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000232 DAJTRQ010000000
VERSION     DAJTRQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.236"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(153..1994)
                     /locus_tag="OO332_004094"
     CDS             complement(153..1994)
                     /locus_tag="OO332_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5944326.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(1994..>3006)
                     /locus_tag="OO332_004095"
     CDS             complement(1994..>3006)
                     /locus_tag="OO332_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5944327.1"
                     /translation="SSPEAMQSWLQNNANELARVGVDPNSVAQMYQQNPSGFGEFVDH
                     LGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQARGQNLSYQSAMTGHN
                     IAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGIETAVNNATASSNSANS
                     MSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQNALVNSQVLKFLPPGP
                     ATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNEFKAEWMSANGNPGQSR
                     NGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEPRNQVGSYTSKSGIQFT
                     VE"
BASE COUNT          676 a          755 c          657 g          918 t
ORIGIN      
        1 gctgtaatgt ctgtcattta atttgctcca gatacaatga atcgccgcag cgtggctacg
       61 gtgaattttg ggcataaaaa aaaccagccg aagctggata gttgcgttgg ttatctgtca
      121 gtagttatgt gcttatttaa ttatttatct atctattgtt ctgctgtaat ttggcttgaa
      181 agtatagggc gtatcgcatt tgcagcatta tttaacgctc tttcataagc tgccgttcct
      241 gctttggtgt ttgccaaacg aagaagcata ttccttgcgg ctttggactc atacaaacgc
      301 atcattgcac caaaaccagc ctcaagcccc attgatacac caagagtcgc agttgcgcca
      361 atcgtcctta tcctgttggc ttgcgattgc cccgtctgag ttactacatt tgcggtgtct
      421 gaccttgctg tttgctgtag aacttcatga agagcatcaa gctctttcat gtgctttcca
      481 gaaaaaatag tgttgtaaat ttcaccgcct gactgagatt tcagcttatt aacttcagtg
      541 atgaacttgg ctggagagtc accggccttt tccgctattt tgctgacgta agctgcacgc
      601 atagcatctt tccctttatc atccagtgcg ctccagattc gtttcacgtc agatggtttt
      661 ctgcttaata caacggtatt tataagttca ggactggctt cactgcttgc cttgttgagc
      721 ttgttagcaa tgtttttatt aagcacctta ttataaacgt ttgcataatc ggaatttgct
      781 ttaaggtatt ttgctgcgtc tgatgcaccg aggtttttag caactgcgtt acgaaggtct
      841 tttgacattg cattctctac catattggta gctgcttttg cctggttggg gaagaccata
      901 gcatctccct gaacattaga tctaaatgct gttctgtgct gacgcaagag atcaaacgta
      961 acatccaaat cagttgcagg gtttgctaat tcttcacgta ggttacgcaa ggatgtaagc
     1021 aggctttgat tggcagacgt cccaagccgt tcctgtcttg cgatcgctgt attcagagca
     1081 ttcatggtat ttgtggtatc aactgcggca ttacccattt tattggtgac gtcattgata
     1141 acagcgccag cggcatcctt ccgccccctt aacgtggtgg taagagattt caccacatca
     1201 tcagggttgt actcaccaaa acggtcaaaa taattactta ccagcttact acgcgttgca
     1261 tattgctccg ctcgctttga gcctgtcccg agcaaagccc cctcggcatc ctgagttagg
     1321 ccgcgagtga aagcattttt cggcgggata acatcagatg tcattggtgt cacgcccatc
     1381 gattctgatg tggcaatttt cttcgccact tctggcgcaa tatcaccttt tatagccgtt
     1441 attccacgcc ctattccctt tgctgctgcg gaaagaacac cctgagcggc aaggttaact
     1501 ccggcatttt tagctgcatt ttgtgcgaaa tcgcctttct gatttgcggc ctctgccagt
     1561 gatccaatag ccatgcttcc tgccgttcca actcctggaa ctaaataccc accaattgtt
     1621 tcaccggctt gcgcgtaggg gtctgtcggt ctgtctactg gacgataaac atcatccaaa
     1681 accttgggtc caccaagccc ctgactgatt gcattaatca gacttgcgcc gccctgcaat
     1741 acgtcaaatg gtatgtttac cagaccacga ccagcctgtt ctgcaatttg ccctgcactt
     1801 tgaccaccag tgagccaatc gccagcttgt tgcatcaatg atggttcttc ccgtgttggt
     1861 gcattattgg cctgattaac tgtttgttgc tgaacagcct gaccagcaaa atactcatca
     1921 atggcggtgc caatatcttc ggtgctcgta ccatcaggaa aggtaaatgt cttaccgttt
     1981 gcagttactt tcatcattcc accgtaaatt gaatgcctga ttttgaggta tatgatccaa
     2041 cctgattccg tggttctcct gaaggtgtcg aatcttgtgc tggcgctgcg tcagtattca
     2101 ttgacatata ccgcttaacg gcactcccca atgattcacc ttttttaaca tccaacccca
     2161 atatctgacc gccattacgc gattgtccag ggttgccatt cgcgctcatc cactcggctt
     2221 taaactcatt aaactgcgcg tttcgtcgct caaggtttgc cattgcatca agccatcttg
     2281 cgaccgtctc agggttatcc atgtcagttg gcgcaccctg tcgaacgatc tcaacgtctt
     2341 tatccgttgc tgggccggga ggtaggaatt taagaacctg actgttaaca agggcatttt
     2401 ggcgaatgcg caaatcacgc aatgttgtat cgcttccggt aagttttgcg aacatgttct
     2461 gtgcgttacc gaacaaacct gtcgttggtt tttctgctct gaactgttga gcaagcgcac
     2521 tcatagaatt ggctgagttt gatgatgctg tagcattgtt tacagccgtc tcgatgcctt
     2581 tttccatgtt tactgacagc ttaggtgctt cgctaatcaa ctgctgagcc ttttcctgcg
     2641 cttgctgcat cttaaacccg aactcttgct gatccagagc caagcgttgt gctgcgatat
     2701 tgtgcccagt cattgctgac tgataggaaa ggttttgccc tctcgcctga agtgcttctc
     2761 cagcctgatt gctgcggatt gtctctgcca gcctgcctcg gtcaatttca cgaccagcca
     2821 tcttgtcctg aacattgaag taatcaatcg gaccaagagc agccattcca aggtgatcaa
     2881 caaactcacc aaatcctgaa ggattctgct gatacatctg agcaacgctg ttagggtcaa
     2941 caccgacgcg cgccagctca ttggcgttgt tttgcagcca tgattgcatt gcttctggag
     3001 acgagg
//
LOCUS       DAJTRQ010000233         2872 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000233 DAJTRQ010000000
VERSION     DAJTRQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..460)
                     /gene="intS"
                     /locus_tag="OO332_004096"
     CDS             complement(<1..460)
                     /gene="intS"
                     /locus_tag="OO332_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5944328.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(622..696)
                     /locus_tag="OO332_004097"
     tRNA            complement(622..696)
                     /locus_tag="OO332_004097"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Arg,seq:cct)
     gene            complement(772..1704)
                     /locus_tag="OO332_004098"
     CDS             complement(772..1704)
                     /locus_tag="OO332_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5944329.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1998..2753
                     /gene="mlaA"
                     /locus_tag="OO332_004099"
     CDS             1998..2753
                     /gene="mlaA"
                     /locus_tag="OO332_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5944330.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          695 a          697 c          689 g          791 t
ORIGIN      
        1 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
       61 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      121 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      181 taaatgaatt gttgttagac gaagcctttt tcgcctttac cggatcgatg ccagccgaga
      241 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      301 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      361 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      421 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      481 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      541 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      601 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      661 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta
      721 atggactgcg gggtaagtac gctgccgctc gattgcttaa accctcgcca tttatgctgg
      781 gtttttatca tttttcttaa tgttttccgc acgttctgct ttttggcgtg cttctgcttt
      841 acgcttgttg ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt
      901 gccgccgcag atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca
      961 gtgcagcgta ccgttaaaca ccagatagag gatttcaaca gaaccgacca cgatatgggt
     1021 ggtgtcaccc agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc
     1081 tgctgcagga aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc
     1141 aaacatctcg ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg
     1201 agtttcttca ttgaagatag gcatatattc aaatgcccac gcagcaatac cagtcccgag
     1261 aatattaccc agcagcacga ctccccataa ccgcataagt aagccaacgt tgctcattgt
     1321 cggtttttgc atgacgggta gtaccgcagt cacggtgttt tcggtaaata attgctggcg
     1381 ggccattatg acgataataa aaccaaaggt ataaccgaga ttctccagca agaagctgcc
     1441 cggcacacct tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga
     1501 cagacccgcc gcaatggctg accacagtag cgccattgcg tcgcgttcta gctctttttc
     1561 accatcctgg cggatatgct catgaattac catcgctcgg gaggggagtc ggtcttcatc
     1621 tatttctatt tttttgccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg
     1681 ttgatcaatt ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc
     1741 gtagcggctt ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggttgcg
     1801 tttgcctttt tctgtttcta tagaatcaag tagcctacag ggcggcgatt accaggctat
     1861 gatcaaatca gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac
     1921 acctaaacag gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa
     1981 aaaacaggga gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc
     2041 tggtggggtg tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt
     2101 tcaaccgcac catgtacaac ttcaacttca acgtattaga cccgtatatt gttcgaccgg
     2161 tcgctgtcgc ctggcgtgat tatgttccgc aaccggtgcg taatggtttg agcaacttta
     2221 ctggcaacct tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc
     2281 aggggatggt ccactttacc cgctttttcc tgaacaccat tttggggatg ggcggcttta
     2341 ttgatgttgc agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta
     2401 gtacgcttgg tcattatggc gtgggttatg ggccttacgt tcagttaccg ttctacggta
     2461 gcttcacgct gcgtgatgac ggtggtgata tggcggatgg tctttacccg gttctttcct
     2521 ggctgacctg gccgatgtct gtgggtaaat ggacgcttga agggatcgaa acccgcgctc
     2581 agctgctgga ttccgatggt ctgctgcgtc agtcgtccga tccttatatt atggtgcgcg
     2641 aagcgtactt ccagcgtcat gatttcatcg ctaatggcgg cgaactcaaa ccgcaggaaa
     2701 atccgaacgc gcaagcgatt caggatgatt taaaagatat cgattctgaa taagaaacaa
     2761 ataaaaaagg tgagtcgcaa aactcacctt ttttgttgtt atccctcaat taccacaagt
     2821 aaaaggagat aaaccgacaa tgacccggac gtaatgatgt aaaaatactg at
//
LOCUS       DAJTRQ010000234         2665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000234 DAJTRQ010000000
VERSION     DAJTRQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.238"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..401)
                     /locus_tag="OO332_004100"
     CDS             complement(<1..401)
                     /locus_tag="OO332_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944331.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDE"
     gene            complement(953..1669)
                     /locus_tag="OO332_004101"
     CDS             complement(953..1669)
                     /locus_tag="OO332_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944332.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            complement(2253..2665)
                     /locus_tag="OO332_004102"
                     /pseudo
     CDS             complement(2253..2665)
                     /locus_tag="OO332_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          810 a          590 c          504 g          761 t
ORIGIN      
        1 atctcatcgc ttacagccac tacaggacgt ttccgtatcc tgtgaattat catcccacgt
       61 ggactgcata tatttttaat acacacaacc cctcttccag ccacatgcag tgtcgaggtg
      121 gtcccgcaga ttccgtatac ccgacaccct gcacgggttc cgagtatctg tactcccggt
      181 ggtttatcag agtaatagta gtcactgtta cattcgaccc agctctttcc cccgggggga
      241 tgcgggacca ctcggaaata caccccatat ggtccgtgca cattaatctc cgcatagcaa
      301 tgttctgttg aattacgtgg catagccagt aaagtctctt ttgcagcagc gggtaccgca
      361 taccacatac acattagtgc aactatcagc gcagtattca ttttcattgt cactttccgt
      421 tactggtgct gtccgtcaca gcacctcatg tcctgcggac agggcaagct gaccggccat
      481 aacaactttc agttccatca gggaagtgga agctagacgc cggacaatca aatcccgccg
      541 gcggtacacc ttcttcgcag cagagttgtt cagtcaacat gaaagtgaca gacagtgtca
      601 ccacgatgca gcatcagtat gacggtgaat cgccggtcgt agtttttgtt ctttgtttta
      661 tagggagctt tctgcatcag gcatcgttca tcacggggta ttggtgcaat gctcggcatc
      721 actcagtccg gctggtgatt tgtgatgttt ggcaatttat ctgattacat aatccggact
      781 gagtcccccc tgagtgtttt actgttccga gcacttattt agtaactttc ttgcatagtt
      841 gtttctggct ttaattgtgc tgccctaagg tttgtttctc agtatgacag ctcaatacat
      901 atacttcgaa cgcattatat attcatttaa ttcactttgt gggagaggat tattactttc
      961 tgacataaac atcattgatt tcataaaatc ccgactgttc tgtattaaat attaagaatc
     1021 tgttactgtc atctttatat gaactagaca aaacgagccg tgaatcataa cgagcaagaa
     1081 cataatacca actattatta taacaaagca tctgatattc tttttttaat tgaggtttat
     1141 accagccaac aagaaaggaa aaaagccaaa aataaaccaa aaaaccagcc ataattaact
     1201 taatgcggtg ttttctaaca aaagggatat ctgaaaaaca tgatacagaa aagatccgtc
     1261 catatatacg aattattatt gaaataacca atgaactaat aatgcatgaa agtgataatg
     1321 gaagtatgaa atattgtttg agaattaaaa gttcgaatat tacagggaaa aacagtaggg
     1381 taatagcaaa aaatagtcta aaaaaactca agtcttgtat tttacttctt ttctttataa
     1441 gaagaaaaaa taaaacacca atcccccaac cggtaaaaag tatacccatt actatgatag
     1501 catagagtaa acttctggca acatcatcca aacctgcacc aacaacccac catggaaatc
     1561 catagtaaaa agaggttccc cagccataag aataagcact gacccaccct acacatccca
     1621 tataggcgat gaataatgaa gaatttctaa gcaatgaatt gtcattcata agaaaccaac
     1681 tgataatgta aaaaatattc gagataaaag ttgatctttt aatttttatg ctaggaaatt
     1741 aacaataata tcattgttaa tgcatattta tcatatcaaa atactctgca gaatgctact
     1801 atcaaaacca gacaagtgaa caaaacttgg tcaacattta gtaacgctag ttggtaatgt
     1861 cttcaatcga tagaagtttc tggtaatcta ctggtattca aacagttagg gctcatacag
     1921 cttgctggac cttccccata accgcccgaa cgtgaaggtc tgatgtgttg caagagagcc
     1981 ggatgcggga aatctgcaag tccggttcga tgagagggac gttgttttta cttgttgttg
     2041 atgaagactt tcattattga acagaatgat aatattgtct aaacaaaaca ttaaatctca
     2101 taattctttg tagaaaacgc ttttcttgaa attaaaaata agtagaatca gcagtctcat
     2161 tataataaca gacaatgtca tgctctcgcc cgttccggta ataaacggag cgggctatct
     2221 ttttggaata tttatggccc acaatccagt tttcataaat acgattcatt ccattcttcg
     2281 ttttttctga gcattgcgtt cataatggtc agcaacttac gcatacaggc aaccagtgcc
     2341 acttttttgg cgtttccggc tgcaagtagg cgcatgtaaa acgcctttat caccagatta
     2401 aaccgggggc ctcaagcgcc gccatgtaca gagctgttct tccccagcac aatcaccgaa
     2461 aatggttcgt cgaccgcgca gagttcccga gtcccggttt acctgagcaa caccgacaag
     2521 tgcgctgatt tcacgtctcg agaggctgct cgattcaggc atttccgcca gcaatgctgc
     2581 ggcagtcatg gctctaacac ctcagacgtt cagtaagctc tttaaagtaa ctctgaatgt
     2641 gcctgttcat atctgggtct tcccc
//
LOCUS       DAJTRQ010000235         2655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000235 DAJTRQ010000000
VERSION     DAJTRQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..191
                     /locus_tag="OO332_004103"
     CDS             <1..191
                     /locus_tag="OO332_004103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944333.1"
                     /translation="RDLTAVPVAHPAKFWQKNTAVFLIELNALRIAECIILSPLFESG
                     ERRPLLEKVFAGLLRVFQ"
     gene            complement(129..434)
                     /locus_tag="OO332_004104"
                     /pseudo
     CDS             complement(129..434)
                     /locus_tag="OO332_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            502..1117
                     /gene="tnpA"
                     /locus_tag="OO332_004105"
                     /pseudo
     CDS             502..1117
                     /gene="tnpA"
                     /locus_tag="OO332_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1445..2527
                     /gene="ompC"
                     /locus_tag="OO332_004106"
     CDS             1445..2527
                     /gene="ompC"
                     /locus_tag="OO332_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5944334.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          766 a          585 c          561 g          743 t
ORIGIN      
        1 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc
      121 tttttgaatc aggggaacgc cgaccgcttc tggaaaaagt ttttgcaggc ctgctcaggg
      181 tctttcagtg actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg
      241 gcatttttcc actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg
      301 gccttatgat tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc
      421 tgtagtcgtt tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc
      481 ggaaggcact gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc
      541 cagatttttg accatgacgc gacagaaaaa ctacgtactt acttttcaaa tgtatgtgct
      601 gattttgaag ctgaactggt tgaaatggat ggcgaaccag agcacgtcca tctgttaatt
      661 aactatcccc ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc
      721 gggtaggtta ctgcgacggg atcggccaga tattgctgcc aggtattacc gcaaagatgt
      781 tttgtggcgt cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
      841 ccaatacatt gaacaacagc aaacacctgg tcaggtgaaa aaacgagcct tatatccccg
      901 ccctgaagga cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat
      961 ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag
     1021 agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga
     1081 actaatatca agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa
     1141 atctaaataa atatcataca aataatttcc atggttattt ttaggctatt taaattaaaa
     1201 aaataaatta tgagtcaatc ataaataaca acctttaata taatttgact accaaattta
     1261 tattcgttca caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg
     1321 gatctacttc acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat
     1381 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
     1441 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat
     1501 gtctgctcag gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa
     1561 agttaatgct aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt
     1621 tcgtctgggc ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg
     1681 ggaatatgaa ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg
     1741 tcttgccttc gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta
     1801 cggtgtagca tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac
     1861 ctggacccaa acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa
     1921 caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa
     1981 tgaccgcact gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga
     2041 gtatgaagga ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt
     2101 tatctacggt aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg
     2161 tctgaaatat gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat
     2221 gactgttttt ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca
     2281 atatcagttc gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga
     2341 cttgggtgcg tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta
     2401 cttcaacaaa aatatgtcca cttttgttga ttacaaagtc aacctgattg ataagagcga
     2461 tttcacgaaa gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca
     2521 gttctaatct gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt
     2581 ggcgccctca agccactttc cttagaagca ctatcttgct tctgactata taaattaccc
     2641 tttattttct gggac
//
LOCUS       DAJTRQ010000236         2611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000236 DAJTRQ010000000
VERSION     DAJTRQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.240"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..564
                     /locus_tag="OO332_004107"
     CDS             <1..564
                     /locus_tag="OO332_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5944335.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            522..1199
                     /gene="bglJ"
                     /locus_tag="OO332_004108"
     CDS             522..1199
                     /gene="bglJ"
                     /locus_tag="OO332_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5944336.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="OO332_004109"
     CDS             complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="OO332_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5944337.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2166..2402
                     /locus_tag="OO332_004110"
     CDS             2166..2402
                     /locus_tag="OO332_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5944338.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2441..2527)
                     /locus_tag="OO332_004111"
     tRNA            complement(2441..2527)
                     /locus_tag="OO332_004111"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2491..2493),aa:Leu,seq:cag)
BASE COUNT          665 a          583 c          671 g          692 t
ORIGIN      
        1 gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga acattattat
       61 tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg ctcctggtat
      121 tcccaggctg tagaactgct catgtgccct acggcgacgt tattgtctga tgttgaaccc
      181 attgagaatc tggtcaagac cgtacgttcc ggcaatacgc atgcagagcg tatcagcgcc
      241 atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc cgtcattctc
      301 actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat caaccagata
      361 gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag tgcgatgcgt
      421 cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc gcagggtgca
      481 agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac agccgaatta
      541 agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag tagtatcggt attgagagtt
      601 tattcagaaa gtttgcgggc aacccttata agctccatac ctataccagt caggagtcat
      661 ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt tctgccatga
      721 gaagtgagcg gagagaggga ttatcttgcc tgactgagct ggcgattaag tttccgcgta
      781 cccggcgttt agttattgtg gatgatgata ttgaagctcg gctgattggt tcattgtcgc
      841 catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt catcaggaat
      901 tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat cagtggtaca
      961 ttaaccaaag ccggacgtta agcccgacgg agagagaaat attgcgcttt atgtcgcgtg
     1021 gctactcaat gacacaaatt gccgagcagc ttaaacgtaa tatcaaaacg atccgagcgc
     1081 ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg ttggaggccg
     1141 cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat ccctattaat
     1201 ccgcatgatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac aatagccaca
     1261 ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca gcaggccgtc
     1321 gcgcagtacc acggtacgcc agagtggatt atcttcaccg ttcgtgagcg ttttctcaaa
     1381 aaagagggca tggcgcagcg attcaacagt agcctcgccg agcagttgct tcatctcagt
     1441 gagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt tgatctctcc
     1501 ggtcgcttct agtgcttgca caaccggaac cagcgcctgg ctgattaacg tttccattcg
     1561 ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc agaaacaggc
     1621 gacgcgtccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat ctaatgcttt
     1681 ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat accattgtgc
     1741 ccatagtgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc gatagatatg
     1801 atcggaatag accgccagta gagaacttag cgcattcggt gatgaccatt gcgccagcgt
     1861 catggcgtta agcggagcgg gttcatccag gcggataaac tccagcaaat gttcacgatg
     1921 tttggcgatc atcgcccgta cggcttgtgc aagcccagca tcctgcggct ggagatgcgt
     1981 tcgccagatg acatcttcat agagcggtgc ggaacgatag gccataatcg tgatagtaat
     2041 ctaaatgata atgattgcta atcctatcga taggtttact cgatagcaag ggatttctct
     2101 ggcttgcaaa tgataaaaat tatcatatga tattggtttt cattatcaat gaaagagatg
     2161 aaaccatgtt gcaacgtacg ctgggcagtg gctggggagt gttgctgccg ggattgctga
     2221 ttgcagggct gatgtatgcg gatttatcgc cggatcagtg gcggattgtc attctgatgg
     2281 gattagtatt gacgccggtg atgctgtatc acaaacagtt gcggcattac attttgctac
     2341 catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg aatcagggat
     2401 gacaaaacag ggaagaaaca agaaaggaag taaagataat tggtgcgagg ggggggactt
     2461 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
     2521 ccttcgcaca gtcatcttac tttttttgat atcgcctcgt ttggtgcgag gggggggact
     2581 tgaaccccca cgtccgtaag aacactaaca c
//
LOCUS       DAJTRQ010000237         2600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000237 DAJTRQ010000000
VERSION     DAJTRQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.241"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            190..447
                     /locus_tag="OO332_004112"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO332_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO332_004113"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO332_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5944339.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO332_004114"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO332_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5944340.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          668 a          597 c          619 g          716 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg
//
LOCUS       DAJTRQ010000238         2575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000238 DAJTRQ010000000
VERSION     DAJTRQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1623
                     /gene="kup"
                     /locus_tag="OO332_004115"
     CDS             <1..1623
                     /gene="kup"
                     /locus_tag="OO332_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5944341.1"
                     /translation="ILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGEVVITPAISVM
                     SAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLILAGLG
                     LRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGVEALYADMGHFGKFP
                     IRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALATVIA
                     SQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFE
                     HSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFTANL
                     DKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPVRVP
                     GTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPT
                     FWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKRPWYLRLRGK
                     LYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            1790..2209
                     /gene="rbsD"
                     /locus_tag="OO332_004116"
     CDS             1790..2209
                     /gene="rbsD"
                     /locus_tag="OO332_004116"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5944342.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2217..>2575
                     /locus_tag="OO332_004117"
     CDS             2217..>2575
                     /locus_tag="OO332_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5944343.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          582 a          623 c          675 g          695 t
ORIGIN      
        1 atcctgacgt tgatgtcgct tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg
       61 gtgattatgg ggctaatcgg cggcagcttt ttctatggtg aagtcgtcat aacacccgct
      121 atttcggtga tgtcagccat tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg
      181 atagttcccc tctcaattat cgttctcact ttattattta tgattcaaaa gcatggcacg
      241 gctatggtcg gtaagctgtt tgcgccgatc atgctgacct ggtttttgat tctggcagga
      301 ctggggttac gtagcattat tgctaacccg gaagtgctgc atgcgctgaa tccgatgtgg
      361 gcggtgcatt tcttccttga atacaaaacg gtttctttta ttgcattagg ggcagtggtg
      421 ctgtcgatta cgggggtcga ggcgctgtat gctgatatgg ggcactttgg taagttccct
      481 attcgcctgg cgtggttcac cgtcgtatta ccttccttga cccttaatta cttcggtcag
      541 ggagcgttgt tgttaaagaa cccggaagcg attaagaacc cgttcttcct gttggcaccg
      601 gactgggcgc tgatcccact gctgatcatc gccgcactgg cgacggtaat tgcctcgcag
      661 gcggttatct ctggcgtctt ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg
      721 ccgatgcgca ttattcacac ctccgaaatg gagtcagggc aaatctatat tccgtttgtg
      781 aactggatgc tctatgtcgc ggtcgtgatt gtgattgtca gctttgagca ctccagcaac
      841 ctggcggcgg cgtacgggat tgcggtgacc ggaaccatgg tactgacgtc tattctctcg
      901 actaccgtgg cacgtcagaa ctggcactgg aataagtatt ttgttgcgct gatcctgatt
      961 gctttccttt gtgtcgatat tccattgttc accgctaacc tcgataaact gctctccggc
     1021 ggctggttgc cattgagcct cggtactgtg atgtttatcg tgatgaccac ctggaagagc
     1081 gagcgtttcc gcttgctgcg gcggatgcat gaacatggta actctctgga agcgatgatt
     1141 gcttcgctgg agaaatcacc gcctgttcgc gtgcccggga ccgcggtgta tatgtcgcgt
     1201 gcaatcaacg tcattccctt tgcgctgatg cataacctta aacataacaa ggtattgcat
     1261 gagcgggtga ttttgttaac tctgcgcacc gaagacgcgc catatgtcca taacgtccgt
     1321 cgggtacaga ttgaacaact gtcgcccact ttctggcgcg tggtggcaag ttatggttgg
     1381 cgagaaacgc caaacgtgga agaagttttc caccgctgcg gtctggaagg attaagttgc
     1441 cggatgatgg aaacctcctt ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg
     1501 tggtatttgc gtctgcgcgg caagctgtac ttgctgctgc aacgtaatgc gctgcgtgca
     1561 ccagatcaat ttgaaatccc gccaaacagg gttatcgaac tgggtactca ggtcgaaatc
     1621 taacgccaga cgcctccttt cttcataagg gggcgttttt gttttcatgg ttaatcacca
     1681 tgtaaaacgt ttcgaggttg atcacatttc cgtaacgtca cgatggtttt cccaactcag
     1741 tcaggattaa gctgcggatc agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg
     1801 caccgttctt aattctgata tttcatcggt gatctcccgt ctgggacata ccgatacgct
     1861 ggtggtgtgt gatgctggtt tacccatccc caaaagtaca acgcgtatcg atatggcatt
     1921 aacccaaggt gtaccttctt ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt
     1981 cgaggctgcc attatcgcgg aagagatcaa acaacataat ccgcaactcc acgaaacgtt
     2041 gctcactcac cttgagcagc tgcaaaaaca ccagggaaat accattgaaa ttcgttacac
     2101 cacgcatgaa caattcaaac aacaaaccgc agaaagtcag gcggtaattc gcagcggaga
     2161 atgttctccg tatgcgaata tcattctctg tgctggcgtg acgttctgag gccgtcatgg
     2221 aagcattact tcagcttaaa ggcatcgata aagccttccc gggtgtaaaa gccctctcgg
     2281 gcgcagcgtt aaatgtctat ccgggccgtg tgatggcgct ggtgggcgaa aacggcgcgg
     2341 gtaaatccac catgatgaaa gtgcttactg gtatctatac tcgcgatgcc ggcacgcttt
     2401 tatggctggg gaaagaaacg acatttaccg ggccgaaatc ttcccaggaa gccgggattg
     2461 ggattatcca tcaggaactg aacctgatcc cgcagttgac cattgccgaa aacattttcc
     2521 tcggtcgtga gtttgttaat cgctttggca aaattgactg gaaaaccatg tatgt
//
LOCUS       DAJTRQ010000239         2489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000239 DAJTRQ010000000
VERSION     DAJTRQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.244"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..163
                     /locus_tag="OO332_004118"
     CDS             <1..163
                     /locus_tag="OO332_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5944344.1"
                     /translation="ILGTEFQLEPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTET
                     SRWIQTWPN"
     gene            151..597
                     /gene="etp"
                     /locus_tag="OO332_004119"
                     /gene_synonym="yccY"
     CDS             151..597
                     /gene="etp"
                     /locus_tag="OO332_004119"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5944345.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            617..>2489
                     /gene="etk"
                     /locus_tag="OO332_004120"
     CDS             617..>2489
                     /gene="etk"
                     /locus_tag="OO332_004120"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5944346.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAGISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     "
BASE COUNT          653 a          599 c          653 g          584 t
ORIGIN      
        1 gatactcgga acggaattcc agcttgaacc ttacgacatt gtttatgtca ctaccgcgcc
       61 gctggctcgt tggaaccgtg tgatttctct gctggtaccg acgatttccg gggtgcatga
      121 cctgacagag acttcacggt ggattcaaac atggcccaac taaaatttaa ctcaatccta
      181 gtggtttgta ccggcaacat ttgccgttcg cccattggcg agcgcttgct gcgtaaacgt
      241 ctgccgggcg taaaagtcaa atcggcaggt gttcatggtc tggtaaaaca ccctgcagat
      301 gcgacagcgg cagatgttgc cgctaatcat ggcgtatctc ttgaaggaca tgctggacgt
      361 aagctcactg ctgagatggc acgaaattac gatctgattc tggcaatgga gtcggaacat
      421 attgctcagg ttaccgcaat tgctcccgag gtccgcggga agacaatgct gtttgggcaa
      481 tggctggaac agaaagagat cccggatccc tatcgtaaaa gtcaggacgc atttgaacat
      541 gtctacggta tgttggagcg cgccagtcag gaatgggcga agcgcctcag ccggtaattt
      601 gagtttataa aatacgatga caactaaaaa tatgaatacg ccactaggca gcactcagga
      661 aaatgagatc gatctgcttc gtctggtcgg cgagttatgg gatcaccgta agtttattat
      721 cagcgtgacc gcgttattca cgctgatcgc tgtcgcttac tcgctgttaa gcacaccaat
      781 ttatcaggca gatactctgg tccaggttga gcaaaaacag ggcaacgcca ttctcagcgg
      841 cctgagcgat atgctcccta actcatcgcc cgagtctgca ccggagatcc aactgctgca
      901 atcgcgcatg attctcggta aaaccattgc tgaactgaat ctgcgcgaca tagttgagca
      961 gaagtatttt ccgattgtgg gtcgcggctg ggcgagatta accaaagaaa aaccaggtga
     1021 gctggcgatc agctggatgc atattccaca actgaatggt caggatcagc aactgacact
     1081 cacggttggg gaaaacggcc actatacact ggaaggtgaa gagttcaccg tcaatggtat
     1141 ggtcggccag cgtctggaaa aagatggcgt tgcgctgact atcgcggaca ttaaggccaa
     1201 accaggaaca cagtttgtcc tgagccagcg taccgaactg gaagcgatta acacattgca
     1261 ggaaaccttt accgttagcg aacgcagtaa agaaagcggg atgctggaac ttaccatgac
     1321 tggtgatgat ccccagttga ttactcgtat tctgaacagc atcgctaaca actatttgca
     1381 acagaatatc gctcgccagg cggcgcagga ttcacaaagc cttgaattct tacagcacca
     1441 gttacctgaa gtgcgcagcg agctggacca ggcggaagaa aaactcaacg tttatcgcca
     1501 gcagcgcgat tcggttgacc ttaacctgga agccaaagcc gttcttgagc agattgtgaa
     1561 cgttgataat caactcaatg agctgacctt ccgcgaggca gggatctccc agctgtataa
     1621 gaaagatcac ccaacttatc gtgcgctgct ggaaaaacgc cagacgctgg agcaagaacg
     1681 caaacgcctg aataagcggg tatcggcaat gccttccacc caacaggaag tgttgcgttt
     1741 aagtcgtgac gtagaagcgg gccgtgcggt atatctgcaa ttacttaacc gccagcagga
     1801 gttgagtatt tcgaaatcca gtgccattgg taacgtgcgg attatcgacc cggcagtcac
     1861 tcagccgcag ccagtgaaac cgaaaaaagc gttgaatgtg gtgcttggtt ttattcttgg
     1921 cctgtttatt tcggtgagtg ccgtgctggc gcgtgcgatg ttgcgtcgtg gtgtagaagc
     1981 cccggaacaa ctggaagagc acggcatcag cgtttattcc actatcccaa tgtccgagtg
     2041 gctggataaa cgcacccgtc tgcgtaagaa aaatttattt tctaatcagc agcgccatcg
     2101 tactaaaaat atccccttcc tggcggtgga taacccggcg gattctgctg tggaagccgt
     2161 acgtgcgcta cgaaccagtc tgcatttcgc tatgatggag actgagaata acattctgat
     2221 gatcaccggt gcgacgccag acagtggtaa aacgtttgtc agttcaactc tggcagcggt
     2281 gatcgcccag tccgatcaaa aagtgttatt tattgatgcc gacttacgcc gtggttattc
     2341 gcataacctg tttaccgtga gtaatgaaca tggcttgtcg gaatatctgg caggtaaaga
     2401 tgagctcaac aaagtgatcc agcattttgg caaaggaggc tttgatgtga ttactcgcgg
     2461 tcaggtgcca cctaacccgt ctgaactgc
//
LOCUS       DAJTRQ010000240         2421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000240 DAJTRQ010000000
VERSION     DAJTRQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.245"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..636)
                     /gene="yidZ"
                     /locus_tag="OO332_004121"
     CDS             complement(<1..636)
                     /gene="yidZ"
                     /locus_tag="OO332_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5944347.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQ"
     gene            complement(611..1786)
                     /gene="mdtL"
                     /locus_tag="OO332_004122"
     CDS             complement(611..1786)
                     /gene="mdtL"
                     /locus_tag="OO332_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5944348.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1918..>2421)
                     /locus_tag="OO332_004123"
     CDS             complement(1918..>2421)
                     /locus_tag="OO332_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5944349.1"
                     /translation="MGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSN
                     LAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIG
                     GAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVF
                     NVLPKFG"
BASE COUNT          688 a          644 c          619 g          470 t
ORIGIN      
        1 ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat tccacgtttc
       61 atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa acagcacttc
      121 ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat ggctttcgcg
      181 accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat cgtaatccca
      241 gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg acagcgcatt
      301 aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc cgcgcggtgt
      361 ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg ccagattttg
      421 ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca caaagagcgg
      481 gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag gggtcacgtt
      541 catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca gacagagcag
      601 cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg cgtccaggcg
      661 cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc ccgatcagca
      721 tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac gaaccgcaaa
      781 cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat ggtcctaacg
      841 cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc gtgataccaa
      901 acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca aggaataaca
      961 cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat ggcgtcgaga
     1021 atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat tctccgcgct
     1081 caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg aggatcaccg
     1141 aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc agcgactcgc
     1201 tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct tttaaaataa
     1261 acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac agactctgcc
     1321 acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga atgatgcagg
     1381 taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc gtgtcgcgca
     1441 aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga aatcggcctg
     1501 caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata ataaatagcg
     1561 ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta ccggcaaata
     1621 gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac tgcgcttcgc
     1681 tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca atcccggcgg
     1741 gataaagtaa aaccagggca aaactacaaa tcaaaaagcg ggacatgggc taaagcgtcc
     1801 tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt tgccatttgg
     1861 acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt gaaggattta
     1921 gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca cggttatacc
     1981 aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca tctgattggg
     2041 aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc agatggtggc
     2101 gcacagcccg gcaccgccga tcccgtaaat aaagccgttc gggaagatca gatacaacaa
     2161 cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg agttatcaat
     2221 cttaaacagg tccgccagat aatcgaacaa ccccagcgtg acaccaaaga acgaactggc
     2281 aacagctaag ttagagaaca ccagcaggca aaactcgata atgccgtgct gtttggtgcc
     2341 gaggaacgat ttcaccagcg aatcaacgtt gccgcctgag gagattatcg ccttaaagct
     2401 ttcgcgcgga atattcccca t
//
LOCUS       DAJTRQ010000241         2412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000241 DAJTRQ010000000
VERSION     DAJTRQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(346..612)
                     /gene="ospE2"
                     /locus_tag="OO332_004124"
     CDS             complement(346..612)
                     /gene="ospE2"
                     /locus_tag="OO332_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR5944350.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(794..1195)
                     /locus_tag="OO332_004125"
                     /pseudo
     CDS             complement(794..1195)
                     /locus_tag="OO332_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1624..2412
                     /gene="virF"
                     /locus_tag="OO332_004126"
     CDS             1624..2412
                     /gene="virF"
                     /locus_tag="OO332_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5944351.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          741 a          433 c          446 g          792 t
ORIGIN      
        1 cccccctaag ccgtctatgg gaaggagtct gctgttattc cagagaatgg taattcagtc
       61 atatatttct ccatctatga cttgtcaata ccatcctgat atttgtgggt cagcaatata
      121 ttctactaaa aggtatttaa ggtcattgcg tccctgcact cgaataatgg tgaagaacgc
      181 atgataaata tatcacgata acgttcagta tgtatcgagg atagatggag ttactcctct
      241 ctgaaataga attactgctc tactttttct gtttccagaa attattagta acaaaccctg
      301 atatattatt ttgaaaatgg agcttaaacc agtctgaaat taacttcaga aatcagaata
      361 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
      421 tgtagcagtg aatttcaacg ttttcgtccg gttggcagca ggtatttgta attcgtatat
      481 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
      541 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
      601 ttgtgtaagc atattgcttc cttatgttat attttagtaa agaatttatt ttttacatta
      661 tagtcggcta aatgaagttt actgtaaaaa aacgatactt ttttcgtaag cgatgagatg
      721 tggagccaga aaaatggctg cgttacgtca ttgaactgtc cttgacccac gatattcaga
      781 cagtcgttgt atcttaagtt gacattaccc gctgacgtcg atattccctt ggtgagagat
      841 aacccaacgc actatgcgga tggttttcat tgtaatgctc gatcgccact gcaaggttat
      901 gcaatgccgt tcttggtttc ggcatgaacg cgatgcagtc ttccttcatc gttttcacga
      961 accgttctgc tattccatta ctctgagggc tactgactgc cgtcgtgcag ggctccagat
     1021 tcaactctct ggcgaactgc cgtgtttcat gagccgtata agccgaaccg ttatcggcca
     1081 ctggattggc cgttccggtc ctttatcgcc atatcactcc tgacatgaca tcctgcaccg
     1141 ttgctttgtc ataatctccc gtgcctgctg cccagtctat ggcttcacga tcgcaatatg
     1201 gaacgtaacg ctgtcatcgg gagaatcgtc aatgcggcat tcggttaacg cttaccaata
     1261 tcaacaccaa ccggatttgt ggagcctcca gtctgaaggg gctttatgcg ttccgtatag
     1321 gatattatga tgctggagtt ttgcgaagct ccactccttt ccgaacattt acacagatat
     1381 tgcctaagaa aagtgaaacc ataatatggt tatagtccct ttcagtgcaa atacttagct
     1441 tgttgcacag agaaatagaa gctgcataag ctctttcttc aaaaaatgta aataaagtta
     1501 aatataggaa aaattactta atctatctta ataacggaaa gattttgtat acaatcactg
     1561 ttacagaaat ttcttagtta ctctgtaaac actaaatata gtttggtata ttctgttgaa
     1621 tttatgatgg atatgggaca taaaaacaaa atagatataa aggttcgctt gcataactat
     1681 attattttat atgcaaaaag gtgttcaatg acggttagct caggcaatga aactttgact
     1741 atcgatgaag ggcaaattgc ttttatagag cgaaatatac aaataaacgt ctccataaaa
     1801 aaatctgata gcattaatcc atttgagatt ataagccttg acagaaattt attattaagc
     1861 attattagaa taatggaacc aatttattca tttcaacact cctattctga ggagaaaagg
     1921 gggttaaaca aaaaaatatt cctcctctct gaggaggagg tttctatcga tttgttcaaa
     1981 tctataaaag agatgccttt cggcaaaaga aagatctata gtttagcttg ccttttatca
     2041 gctgtttctg atgaggaagc tttatatact tcgatatcga tagcttcttc tcttagtttt
     2101 tctgatcaga taaggaagat tgttgaaaaa aacatcgaga agagatggcg tctttctgat
     2161 atttcaaata acttgaattt atcagaaata gctgttagaa aacgattgga gagtgaaaaa
     2221 ttaacatttc aacaaatcct tcttgatatt cgcatgcatc atgcagcaaa gcttttattg
     2281 aatagtcaaa gctatattaa tgatgtatca agacttatcg gaatatcaag cccatcttat
     2341 tttataagga aatttaatga atattatggt ataactccaa agaaatttta cttatatcat
     2401 aaaaaatttt aa
//
LOCUS       DAJTRQ010000242         2405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000242 DAJTRQ010000000
VERSION     DAJTRQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            318..1163
                     /locus_tag="OO332_004127"
     CDS             318..1163
                     /locus_tag="OO332_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944352.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
     gene            1410..1631
                     /locus_tag="OO332_004128"
     CDS             1410..1631
                     /locus_tag="OO332_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944353.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            1733..2242
                     /locus_tag="OO332_004129"
     CDS             1733..2242
                     /locus_tag="OO332_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944354.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
BASE COUNT          640 a          635 c          580 g          550 t
ORIGIN      
        1 taagaccgca cgaatataac ggtgggttac ccccaaacga atcggaaaat cgatactgga
       61 aaaactctaa ctcggtggcc agtttttgtt gaccacttca tctatcaccg ctcaccatct
      121 ggggtcctta cagctcgtcc tgacatgcgg tctcgcctta tatgcacatg gagaacatgg
      181 tcactccaga ccaacactaa tcaacattaa ccctattcac tttcgttaag cactttttgg
      241 tgggtccgtt tggatgcatt gaataggtgc ggactccaca tggagacaga aagtgcttta
      301 ccaaaggagc atcctttatg aataccaacg aagttagcgt catcaacctt gaccagcttg
      361 tgtctatgag tagctacgat ttcttgacta aggtcatcaa cccggctcgt gtggaggctg
      421 gggagaagcc tgtccgtaac aacgactttg tgtcccgtat tgaagatgag ctggacggtc
      481 aaaatgtagc ctacgaaatt ttcgtaagct tcgggcagga cgttaaaagc tataccctca
      541 acactgacca gcttctgctg gtcgggatgc gtgagtctaa agctgtgcgt cgtaaggtcc
      601 tagcctatat tcgccagaag gagcaagagg tcctcgaact tcaggcccag caacttcagg
      661 tcgcacagac caaagctatc tgctatgcgc ccggtgctat cgtcctcgac tccaagctgg
      721 actccaagtc aggcgccacg cctaccgagg ttattaaagc tctggacctt aagttgacca
      781 gctctaagat gctgacagct agcgtcctga ctggatatct gaagaccctg tgtcgtccta
      841 acccattcta caccaagcgt gttactgagt ttggcactac ggatatagac ctatcgaccg
      901 gacgtccacg agccatcttc aataagaaag gtgagaccaa cggctactat cgtctacagc
      961 cacagcctaa cgacaaagag cctaccatca agattctacg caagggttat gactggctga
     1021 aggataactg gaaggatatc gctcatgaca tgcgcacatc caatatcaag gttgtccgtc
     1081 gtctggtgga ttctcacctg aatggtaagg ctacaaagtc ccagaaagca gcagcgggca
     1141 aggggtctga ctatgggttc taagcgactc actgatgaac tgattatgga gtcaatagcg
     1201 acaaccatat gtttcattga gagacacggc cctgagcatc ctgcggaccc tatcttactg
     1261 acttctctgc ttcggcatat acacgaagag atggcactga gagtcgacga gcgactgcct
     1321 ctagagatac cagagggtct acctaacttc caagctccta tcaccagtga atttgacgtg
     1381 gatgattggt ctaacgagga cttctaacaa tgacaactaa acaacatatc gacccacgga
     1441 cacccatcgg aaaggctact ctccgttacc gtggtctgcc tacccgtcgc ctcctgtcaa
     1501 tgctggggat gggcgtagag gaccctgagc gtcctttcta tagtcgagac gaactgatag
     1561 acctgctggt cgaccgtgac ctgaacaatc agttacgccg agcgttcgct aagctggacg
     1621 ccactcacta acccctaatt taaacctttt gagatgaccg atgcggggag acccgtaggc
     1681 ataactgtgt ctgcggtacc gatagtctca actctcaaga ggagtaatta ctatgtctag
     1741 caaagctagt gcgttattca acaacaatga ctggaaactc catgtcaccg acatcaatct
     1801 ctacgaaaac actgtgagtc tggacggtca gtcctaccca ctgtccttcg ctattaagac
     1861 cctgattccg ggctacctgt caggactgcc tgccaccagc cgcgagtcca tggaattact
     1921 ggaagctctg gctgaagctg gcgtgaccat tggaaacttc ttcagcaatg acctgatgac
     1981 cgcctaccaa cgtcgccagc agaacaagcg agccgaggct gagcgtatcg ctaaagagca
     2041 gcgcattcag gctgagcgta tgcgtgaaga gaacatgacc gatgcagagt ggcagaaaga
     2101 gctacagcgc cgcgaacagg tcaaagctga gcgccggacc tatggtgaga acctccgcag
     2161 tgctacccat tccgctggtc gctcccgtgc cgctattgtc gctgacattg agtcaggcgg
     2221 taactggatg gacagcctct aagacagaca cacaagtggt cctgacctga tgtcgggacc
     2281 cacagtatct aaaactaatt taggagattg attattatga ccatgattaa agatagctat
     2341 cagttcggca ttgagccagt acgtatcacc gacaccgaca acattcaggt taacgaaggt
     2401 ctgcc
//
LOCUS       DAJTRQ010000243         2221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000243 DAJTRQ010000000
VERSION     DAJTRQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(145..657)
                     /locus_tag="OO332_004130"
     CDS             complement(145..657)
                     /locus_tag="OO332_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5944355.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            852..1616
                     /locus_tag="OO332_004131"
                     /pseudo
     CDS             852..1616
                     /locus_tag="OO332_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1567..>2221
                     /locus_tag="OO332_004132"
     CDS             1567..>2221
                     /locus_tag="OO332_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5944356.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     "
BASE COUNT          636 a          482 c          560 g          543 t
ORIGIN      
        1 agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg gggtagcggt
       61 aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg gaagctggat
      121 tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc taaaactacc
      181 aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca ctttcactgg
      241 atgaagttgc aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg
      301 cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc gttctattgt
      361 attggaacgg ctcgatatta tacatgcaag ttgagggata tgcccaatta ttataccaag
      421 atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc cagcttccca
      481 tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg atgctggtgg
      541 caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa gaagccccca
      601 taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact ccatcaatgt
      661 ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat ttaggcttaa
      721 acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt cgcaggagtg
      781 agtgatgagg aagaattaaa gcaacctatt gtgacaggca acgataacag taaatcacag
      841 gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac cgggccggag
      901 tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct atcaggaaga
      961 gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc
     1021 aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca caaaaaagta
     1081 cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg cgtaccgatc
     1141 ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt ggttatgccc
     1201 gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg ctcgaggacg
     1261 aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt cggtggttgg
     1321 tgtcgctgga agatgctaat ggggactagg aaaggaagat gctgattgaa aatatcagcg
     1381 ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg
     1441 cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact gataaggtgt
     1501 ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat ggagagcgtg
     1561 acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt acgtagtttc
     1621 tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg
     1681 gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt ttgttgtgcc
     1741 cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat tcagatagac
     1801 aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa ccgggttact
     1861 ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa gtcactggag
     1921 gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca gtggaaaatt
     1981 ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa ccccggactt
     2041 gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac tctcatccgc
     2101 aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt tatcgacgcg
     2161 gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc ggagtctgat
     2221 g
//
LOCUS       DAJTRQ010000244         2195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000244 DAJTRQ010000000
VERSION     DAJTRQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            586..2094
                     /gene="ltrA"
                     /locus_tag="OO332_004133"
     CDS             586..2094
                     /gene="ltrA"
                     /locus_tag="OO332_004133"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5944357.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          556 a          547 c          644 g          448 t
ORIGIN      
        1 acccaacaat cccaccgggt gtgacggtgg agaacctgag cggcagtgac ctgcggcatg
       61 cccgcagggt gatgtaaccc gctgacaacg gggattgagg cgagatcact aagccgagat
      121 gatcctcaag gttaagtact gaaaggctga agaacatgaa cccgttaatc cgcctctgtg
      181 ggttgaaaac gtcaccacgg cctacgtgat ctgacaggcc gtgcaggagg aactggcagt
      241 gatacgtaag cactgccggt cgaaggtgtt ttgacatgta tgcgaaacac cggggcagca
      301 gcgtctatca cgcttgcgtt gctgacttct gccaacttgc ggcaagcaag gataaagagt
      361 gcgacgggca gcctcctcag tatgcctgag tccaggcagg taaaccgggg aaggtcagcg
      421 acggatgtta agggggcatg gctccgatga cgcgctggct ggcggagctt ccgtagtagt
      481 ccgcgatggg gaaagcccat tacatggcga agggaagcag tttgaatgtg tttgcgacgt
      541 gaattaactg acctaacgag gtgaagacct ttgataatca gcgaaatgca acgcaagctt
      601 gccacatggg cagtcaccga tccgtcccta cggattcaac ggctgctgcg tctgataaca
      661 caaccagaat ggctggctga agcggcgcgg atcacgcttt catcaaaggg ggcccatacc
      721 cccggcgttg atggcgtgaa caaaacaatg ctacaggcca gactggctgt tgagctgcaa
      781 atcctcaggg atgaattact ctcaggccac taccagccct tgcccgccag acgggtttac
      841 atccctaaaa gcaacggcaa actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt
      901 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
      961 tcatatggct tccggcctga gcgcagtgtc caccacgcta tccgcacggt gaaattacag
     1021 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
     1081 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
     1141 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
     1201 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
     1261 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
     1321 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
     1381 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
     1441 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
     1501 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
     1561 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
     1621 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
     1681 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
     1741 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
     1801 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
     1861 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1921 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt accggttggt ggggcaaggc
     1981 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     2041 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     2101 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     2161 actacgccct gcctcttact ctactcagca catgg
//
LOCUS       DAJTRQ010000245         2188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000245 DAJTRQ010000000
VERSION     DAJTRQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..>2188
                     /locus_tag="OO332_004134"
     rRNA            1..>2188
                     /locus_tag="OO332_004134"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          601 a          478 c          673 g          436 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg ggagcgttct
     1201 gtaagcctgt gaaggtgtac tgtgaggtat gctggaggta tcagaagtgc gaatgctgac
     1261 ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg ttcctgtcca
     1321 acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg tagtcgatgg
     1381 gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga gaaggctatg
     1441 ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag gcaaatccgg
     1501 aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca aatgccctgc
     1561 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
     1621 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
     1681 gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg ctcgtggagc
     1741 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
     1801 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
     1861 ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
     1921 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
     1981 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
     2041 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
     2101 gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc
     2161 gaccttgaaa taccaccctt taatgttt
//
LOCUS       DAJTRQ010000246         2069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000246 DAJTRQ010000000
VERSION     DAJTRQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.251"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1338
                     /gene="mppA"
                     /locus_tag="OO332_004135"
     CDS             <1..1338
                     /gene="mppA"
                     /locus_tag="OO332_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5944358.1"
                     /translation="IWTFTLRDNAKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAA
                     LAGINNAQAIIDGKATPDQLGVTAVDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANV
                     ESGKEWTKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESA
                     ATKRYLAGDIDITESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVR
                     LALSMTIDRRLMTEKVLGTGEKPAWHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLL
                     SAAGYGPQKPLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNT
                     GNFDVIRASWVGDYNEPSTFLTLLTSMHSGNISRFNNPAYDKVLAQASTENTVKARNA
                     DYNAAEKILMEQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1389..>2069)
                     /gene="ynaI"
                     /locus_tag="OO332_004136"
     CDS             complement(1389..>2069)
                     /gene="ynaI"
                     /locus_tag="OO332_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5944359.1"
                     /translation="MSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGK
                     DILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNS
                     LFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVY
                     FNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDN
                     ITPPEQGR"
BASE COUNT          601 a          478 c          493 g          497 t
ORIGIN      
        1 atctggactt ttaccctgcg cgataacgca aaatgggcgg atggcacacc ggtaacggcg
       61 caagattttg tctacagctg gcaacgtctg gtggacccaa aaacattgtc gccatttgca
      121 tggtttgccg cgctggcggg aatcaacaac gcacaggcga ttattgatgg taaagctacg
      181 cctgatcagc ttggcgtcac cgcagttgat gcccatactt tgaaaattca gcttgataaa
      241 ccgttgccgt ggtttgtgaa tttaaccgct aactttgcct tcttcccggt gcaaaaagcc
      301 aacgtagaaa gcggtaaaga gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat
      361 gttcttaaag agcgcgtagt caatgaaaaa ctggtcgtgg taccgaatac ccattattgg
      421 gataacgcca aaacggtgct gcaaaaagtg accttcctgc caattaatca ggaatccgca
      481 gccactaagc gttacctcgc gggggatatt gatatcaccg aatccttccc gaaaaatatg
      541 tatcagaagc tgttgaagga tattccgggg caggtttata cgccaccgca gctcgggacc
      601 tattattatg cctttaacac gcaaaaaggg ccgacggcag atcagcgcgt tcgcctggca
      661 ttaagtatga cgatagatcg ccgcctgatg accgaaaaag tgttggggac gggcgaaaag
      721 ccagcgtggc attttatacc agatgttacc gccggattta cgccggaacc ttcgccgttt
      781 gaacaaatga gtcaggaaga actgaatgcg caggcaaaaa ctttgttgag cgcagctggt
      841 tatggtccgc aaaaaccgct gaagctgacg cttttgtata acacttcaga aaaccatcaa
      901 aaaattgcga ttgctgtagc atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg
      961 caaaatcagg aatggaaaac ctatatcgat agccgtaaca ccggcaattt tgatgttatc
     1021 cgcgcctcat gggtggggga ttataatgaa ccctccactt tcctgacatt attaacgtca
     1081 atgcattcag gaaatatttc acgctttaac aatccggcat acgacaaagt tctggcccag
     1141 gcatcgacgg aaaataccgt taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc
     1201 ctcatggagc aagcaccgat tgcaccaatt tatcaatata ccaatggacg attaatcaaa
     1261 ccgtggctga aaggttatcc cattaataac cctgaagatg tagcgtacag tcggactatg
     1321 tatattgtga agcattgatg tgatgggaac tggcgttacc cttgtggata acgccagtga
     1381 tgtctgattt agcgaccctg ttccggcggt gtaatgttat ccatatacag cgtctggctg
     1441 gggaaggcaa agtccgcgcc gtgtgactgt acaatatcga taatcttcaa ataaacgtct
     1501 tgctgtgcag caagccactc agcccatacc gtggttttgg taaagcaata aaccataata
     1561 ttcaatgacg agtcagcaaa ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg
     1621 gccgggtgat ttttcagcat ctcacgtacg gcttcgacaa taacgcccac ttttgccgca
     1681 tcctcataac gtaaaccaat ggtcgtggta atgcggcggt tggtcattcg tcctgggttt
     1741 tctacgctga tcgacgaaaa cagcgagttc ggtacgtaca atggacgatt atcaaaggtc
     1801 gtaattttgg taattcgcca gccaatttcc gctactgtac cttcgatatt tctgtccggt
     1861 gaacggatcc agtcgccaat actgaaagga cggtcgaaat agagcatgat cccggaaaag
     1921 aaattactca gaatatcttt accggccata ccgacagcca gaccaccaat accaccaaag
     1981 gtcagcaagc cagaaaggct catgccgaaa tgttcgccat aaagcagaac aagcactaca
     2041 ataatggtga ttttgataat gcgcgacat
//
LOCUS       DAJTRQ010000247         2050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000247 DAJTRQ010000000
VERSION     DAJTRQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..169
                     /locus_tag="OO332_004137"
     CDS             <1..169
                     /locus_tag="OO332_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5944360.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            154..792
                     /locus_tag="OO332_004138"
     CDS             154..792
                     /locus_tag="OO332_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5944361.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..1421
                     /locus_tag="OO332_004139"
     CDS             1020..1421
                     /locus_tag="OO332_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944362.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1432..1906
                     /locus_tag="OO332_004140"
                     /pseudo
     CDS             1432..1906
                     /locus_tag="OO332_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          620 a          366 c          481 g          583 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttt tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg attctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tacaggaaaa cgcctgaggg gctggttgcc tgtagcctgt
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata aaatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagtgggg gggggagatg aaccctatgc aatgtcctgg
     1441 ttaatatctc agtgtgctca tcagtgcact gataataaaa agacggagac agatgccatt
     1501 tacgataaag ttcggagttc ttatttgctt tcatgtattc tgaaaaaaaa caaaaatgtt
     1561 gggcttattc ttcatgcacc atcatttgtt tctgtgtctg aaaaaaatcg ccagaattgt
     1621 gatggcaaat tactcccgta actggagtaa ttccgagttg gccagtgcgg ttttaatgag
     1681 tgaatcttct ttaaaaagaa gaatgtacaa agaggtcggt tctataagta catttgtaca
     1741 taaaataaag cttacagaag ctatcaggaa attacgaagg acaaatactc ccatttctgt
     1801 catatcatct gagttaggtt acagttcacc atcgtatttt tctaaagttt tctttaaata
     1861 tttaaagaca taccctcaaa acataaggaa gaaaaatggt cggtaatgtc accaattagg
     1921 aggacttgca gagccgcttt tttacgatac tgagatcaaa cagacataat ctatgcggag
     1981 atttacagca gggcatggtt cacaaatctg acagcgatga attatcggca ctgcgggcag
     2041 aaaatgctcg
//
LOCUS       DAJTRQ010000248         2025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000248 DAJTRQ010000000
VERSION     DAJTRQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..823)
                     /locus_tag="OO332_004141"
     CDS             complement(<1..823)
                     /locus_tag="OO332_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5944363.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="OO332_004142"
     CDS             complement(1424..1672)
                     /locus_tag="OO332_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5944364.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1900..1965)
                     /locus_tag="OO332_004143"
                     /pseudo
     CDS             complement(1900..1965)
                     /locus_tag="OO332_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          528 a          365 c          478 g          654 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tgattccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cgtttggcat tttagtgatt ttttctttag cgatacagat ctcaacacgc ataacaccct
     1681 cctgtaactg tagttatgta caggtgttat ttttatctgt atggataacc agtgtcaatt
     1741 cctgatattg tttatggggc atcaatgggg catgtatggg acacttttta tcggcgaaat
     1801 tcgtcgaagt tcgtcgacat ggtaaacgaa tcatctatcc aacccttgaa aaacggcgct
     1861 cctggacgat cttcgtcgat ttttaaaaat gttgcgtcac gcgcgtaacg tgacaggatt
     1921 aatatcacaa agcaacgcca cttcaccaat tgtgtaaagc gccatcgtct cacccttgct
     1981 cgcgaggtcc cagtttaact ttagacgcag ttttgcgaac caggt
//
LOCUS       DAJTRQ010000249         2021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000249 DAJTRQ010000000
VERSION     DAJTRQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(209..373)
                     /locus_tag="OO332_004144"
     CDS             complement(209..373)
                     /locus_tag="OO332_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944365.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1275..1559
                     /locus_tag="OO332_004145"
     CDS             1275..1559
                     /locus_tag="OO332_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5944366.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1547..>2021
                     /locus_tag="OO332_004146"
     CDS             1547..>2021
                     /locus_tag="OO332_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5944367.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLK"
BASE COUNT          519 a          422 c          582 g          498 t
ORIGIN      
        1 gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag ccggttactt
       61 taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc tgcaataccc
      121 cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag ctgatggagt
      181 tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg tttccggcgt
      241 aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta tgagagatga
      301 ggggcggcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta agagcggtgg
      361 tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg cggagatgaa
      421 cgaaagtgaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg aaaatgtgct
      481 atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg gcatccggca
      541 tataggcagc atgaacgcaa cacaggctct cgtacggaac gtgggaacct gtcgtctcga
      601 tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga tgcgagacac
      661 aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga gcgaaggggg
      721 tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc atgagtcagc
      781 caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg gtcaaagcta
      841 atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt accaatcgaa
      901 gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg cccgcaaaca
      961 caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa ttcgtcacga
     1021 ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg ttcaacgaca
     1081 agagccgtat gacgcgagag tgtcacgtac ggttccgtgg gagcgtaggg gggcggtgcc
     1141 cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa atgccgctga
     1201 cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt caatatgcta
     1261 ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag ctcaatctca
     1321 gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt cataagtcgc
     1381 gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt ctcgaccggc
     1441 gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt gatgctccgg
     1501 ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg gatgtgtaac
     1561 tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg gtgaagctgt
     1621 acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag ccacccggac
     1681 ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac tggccactgg
     1741 tagtatcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag accccattcc
     1801 tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag ggcttggcgc
     1861 ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata tcggtgtgcg
     1921 tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc atcatggttt
     1981 taagccatca aaaactcagg tgcaaacatt gtttcttaaa c
//
LOCUS       DAJTRQ010000250         1902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000250 DAJTRQ010000000
VERSION     DAJTRQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OO332_004147"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OO332_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5944368.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..>1902
                     /gene="mcbR"
                     /locus_tag="OO332_004148"
     CDS             1351..>1902
                     /gene="mcbR"
                     /locus_tag="OO332_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5944369.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIEN"
BASE COUNT          505 a          403 c          532 g          462 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gggctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga caggaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtgatgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatta
      721 cctcgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg cgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa ac
//
LOCUS       DAJTRQ010000251         1901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000251 DAJTRQ010000000
VERSION     DAJTRQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1072..1584)
                     /gene="clbS"
                     /locus_tag="OO332_004149"
     CDS             complement(1072..1584)
                     /gene="clbS"
                     /locus_tag="OO332_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HCR5944370.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(1619..>1901)
                     /gene="clbQ"
                     /locus_tag="OO332_004150"
     CDS             complement(1619..>1901)
                     /gene="clbQ"
                     /locus_tag="OO332_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HCR5944371.1"
                     /translation="MTIFTPIIKNDYRLYEQYVFQAKARTLTCPIVLFHGDADNLVMQ
                     DELLAWEKFTTRKTRTIIFPAADHFFVDKHFEQVVGYVNQTIESLEIVG"
BASE COUNT          546 a          439 c          377 g          539 t
ORIGIN      
        1 tcgtctgatg gctacatacg gcggtcagtt tacgcttact acaatgatga taatgcgatt
       61 atataaaggt caaaccatat ggttgttagc ggatttcaac aacggtgctg tactcgctga
      121 gcggcacatg aagtttgaat aatgtggaaa caacaaatgg ctacattaag tcattggaca
      181 tatacagaga tggttggcaa atcgagtaca cgatatgttg cctgaaaagt cgaccgaaca
      241 gaactataaa tatcaagtcg gcataacaca tctaaatttc ataagggaag atgtgccacc
      301 ggggacgttt ttttcaccac ggggacttcg ggtataacga tgcaatccga agaaagaaat
      361 ccaatgttgg cgatattcat catcgcttta ttcgtccact catgcgaatg gcaataaagc
      421 gactcaccaa aacagtttct ttagcttccc tctaccaaaa ttccctatga tcgcatgtta
      481 gaactctgcg tgctgaactt cgggccatat ggacggtatc attcatgcac ttgctccttt
      541 gaaattgctg gatatcattc gtctttatct gcaccggccg gcacaaatag ctgtagggga
      601 acccggcgca ggcaaaaaga gcgactttaa cgcgttttgc aggactcccc gttgccttgc
      661 agggagacac tgtatcatca aacaccatct taatcccagt acattaccct ccatcctggc
      721 tcgtaccaaa tcagaggaaa ggcctcgcgg caatagattt tcccggcgaa gctcggagtg
      781 aaatacagtg cagcaatatc cgacatcaga atttgatcct cctgcgtcag gatacgaaaa
      841 aacatcccga tttgagcacc cttgtcgaaa aaaaatcacc ttattatgaa gattggttct
      901 taatacacat gcgttcgcat gtgcttatgt taagtagact tattgtctta cacattttat
      961 ccgtatcatt aaccagaata tgttccggag cagcgggtga tagtgccgcc agtccacact
     1021 catcgctgcc cggaaaaatc atcagtgggg aggcaaacgg taagcacccc gctattctgc
     1081 aagacatttc tgcagtttat ttaacctccc cgaagcattt ttataaggcg aggcggtatt
     1141 aaattgaatc atacgacctc gggtccattt attataccaa ggtttaccgt aaagcttgtc
     1201 gttactgaat cgttcaatca aagccacgag ttgctgctta ttttccttta accgtgccaa
     1261 taaaacttcg tactctgtaa tatcctcata gtcacggtag aatttctgtg ctaaacgccc
     1321 caattcattc catttaaatc cttcctcagg aaaaataata gtttttcctt ttgcctcttg
     1381 gtcatgccag tgtaacacca gctctcccca gccaatcaga taggaaacca gattcgctac
     1441 gctaatcgta gtccccttcg cgtgcccttc caatagaggt tcaaaggcga gttggggcgt
     1501 aatagattct agcttcttat ttaataaaga aaaattacta ttaatagctt taattaactc
     1561 ttcttttgat gatggaacag ccatatctat tgctccttgt atagttacac aactatttct
     1621 accctactat ttcgagtgat tcaatcgtct ggttcacata acctaccacc tgttcaaaat
     1681 gcttatcgac aaaaaaatga tcggcagcag gaaatataat agtccgcgtc tttcgtgtgg
     1741 tgaatttttc ccatgcaagt aattcatcct gcattaccag attgtcagca tcgccatgaa
     1801 atagcacgat cggacaggtt aatgtgcgcg ccttggcctg aaatacatac tgctcataga
     1861 gccgataatc gtttttaatg atgggggtga aaattgtcat t
//
LOCUS       DAJTRQ010000252         1889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000252 DAJTRQ010000000
VERSION     DAJTRQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..214
                     /gene="yfdR"
                     /locus_tag="OO332_004151"
     CDS             <1..214
                     /gene="yfdR"
                     /locus_tag="OO332_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5944372.1"
                     /translation="PVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNV
                     IPLAPGHAYGMFMERFNELSELRKCA"
     gene            205..567
                     /locus_tag="OO332_004152"
     CDS             205..567
                     /locus_tag="OO332_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944373.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            567..872
                     /locus_tag="OO332_004153"
     CDS             567..872
                     /locus_tag="OO332_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944374.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1099..>1889
                     /locus_tag="OO332_004154"
     CDS             1099..>1889
                     /locus_tag="OO332_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5944375.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIG"
BASE COUNT          599 a          409 c          473 g          408 t
ORIGIN      
        1 cccagttatg agtacgcccg tgaaatatgc cgatctcatc atgctggcaa ccgaacgccg
       61 cgatctcggg cttgatgatg gctctttctg gcctgtactg gaaggtatcc cggcaacaga
      121 gatgttcaac gtgattccac tggcaccggg ccatgcctac gggatgttta tggaacgctt
      181 taacgagtta tcggagttac gcaaatgcgc atgaatgttt tcgaaatgga agggtttctt
      241 cgtgggagat gtgtaccgcg agatctgaaa gtaaatgaaa cagatgctga atacctggtg
      301 cgtaaattcg atgcgcttga agctaaatgt gcagcacagg aaaacaaagt aataccagtg
      361 tcaactgaac tgccaccagc aaatgaaagt gttttgttat tcgatgctaa cggagaaggc
      421 tggctaattg gttggcgttc tctctggtac acctggggac aaaaagaaac cggagaatgg
      481 cagtggacat ttcaggtcgg ggaccttgaa aacgtcaata tcactcactg ggcagtaatg
      541 ccaaaagcac cggaggctgg agcataatga ccacttttac cgacaaagaa ctgattaaag
      601 aaattaaaga gcgtatcagc agccttgacg tgcgagacga tattgagcgc cgtgcttatg
      661 aaatcgcact cctatctctg gaagtagaac cagatgaacg cgaagcttat gaattattca
      721 tggaaaagcg tttcggtgac ttagtagatc gtcggagagc aaaaaacggc aataacgaat
      781 acatggcatg ggatatgact ctcggttgga tcgtctggca gcaacgagct ggtatccatt
      841 tttcaacaat gtcacagcaa gaggtgaaat aatggagcca tacagcctca cactcgatga
      901 ggcctgtcat tttctcaaga tatccagacc gactgccatt aactggatac gcacagggcg
      961 tcttcaggca acacacaaag atcccactaa gaataaatct ccttacctca caacacgaca
     1021 agcctgcatt gcggctcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg
     1081 cataacagag gaaagaaaat gtcactcttc cgcagaggtg aaatatggta cgccagtttc
     1141 acattgccga acggtaaaag atttaaacag tctcttggaa caaaggacaa aaggcaggcg
     1201 acagaactcc atgacaagct aaaggctgaa gcatggcggg tcagcaaact tggtgaaata
     1261 cctgatataa cgttcgagga agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa
     1321 tcactggacg atgacaaaag ccggatcgga ttctggcttc aacatttcgc aggaatgcaa
     1381 ctaagagaca ttactgaatc aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt
     1441 catgaggaaa actggaaact cagggcagaa gcatgcagaa aaaaagggaa acctgttcca
     1501 gaatacacgc caaaaccagc gtccgttgta acgaaggcta cgcatctttc atttataaag
     1561 gccctactaa gagccgcaga gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa
     1621 gtgcctcaac caaagaataa acggatccgc tggctggagc cccatgaagc acaaaggctg
     1681 attgatgaat gtccggagcc attaaagtct gttgttgaat ttgcactggc aacaggctta
     1741 agacgctcga acatcatcaa ccttgaatgg caacaaatag atatgcagcg ccgggtggca
     1801 tggataaacc cggaagagag taaatcaaac cgcgcaatcg gcgttgcgct gaatgatact
     1861 gcatgtcgcg ttttgaaaaa acaaatcgg
//
LOCUS       DAJTRQ010000253         1860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000253 DAJTRQ010000000
VERSION     DAJTRQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..560
                     /locus_tag="OO332_004155"
     CDS             <1..560
                     /locus_tag="OO332_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5944376.1"
                     /translation="LGLARSSYFYHRAALKAGDKYATIRKMLTDITEFQLPAGKVWLS
                     PVVDCFDGKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWPGWLE
                     RVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVDAYIRW
                     YNERRIKLSLGAVSPKMYRQQCGLE"
     gene            675..1016
                     /locus_tag="OO332_004156"
                     /pseudo
     CDS             675..1016
                     /locus_tag="OO332_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            999..1836
                     /locus_tag="OO332_004157"
                     /pseudo
     CDS             999..1836
                     /locus_tag="OO332_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          455 a          454 c          571 g          380 t
ORIGIN      
        1 ttctgggcct tgcccgtagc agttattttt atcaccgggc tgcactgaaa gccggtgata
       61 aatacgccac gatacgtaag atgctgacag atatcacgga gttccagctc cctgcaggta
      121 aagtctggct gtcaccagtg gtggactgct tcgatggaaa agttgtgagc tggtctctca
      181 gtacacgccc tgatgctgaa ctggtcaaca ctatgctgga tagcgctgtc gaaacgttaa
      241 atgctggcga acgaccggtg atacacagtg acagaggtgg gcattatcgc tggccaggct
      301 ggctggaaag agtgaatgca gcaggtctta ttcgctcaat gtcccgtaaa ggatgttcac
      361 ctgataatgc tgcatgcgaa ggctttttcg gcagactgaa aacggaaatg tattatgggc
      421 gtaaatggtc gggcatcacg ccagaaaagt tcatgcagca agtggatgct tacatcagat
      481 ggtataacga gcggcgtata aaattatcgc tgggtgccgt cagccccaaa atgtaccgcc
      541 aacaatgcgg gctggaatga taaagcagtc caggaaatcg tccgcatccc cccttcacca
      601 tttggattgt ccgctccacc caacatgttg tttccttaag gttcctacac cacaaaggaa
      661 actctcatgc tgcgcagtgg atggctgacg ttgccgacag gcgggaactt cgccagttca
      721 ggcagacacc ggaacagcgc ttcacgcagg aacaggagca tctgcagccg ctgctgggta
      781 cggacttcga tatccgtcat gtctcctggg atggctatat cgaggttggc gggaaccgtt
      841 acagtgtgcc ggaaagcctg tgtggtcagc tggtatcaat acggatctcg ctggatgatg
      901 aactgcggat ctacagtaat gagcaacagg tagcatcgca ccgtctctgt tcggctgcat
      961 acggctggca gaccgtgccg gagcatcacg ctccactctg gcagcaggct tcggagcgca
     1021 tggccgaacg gctgggggag atacagaaac gggtaatcac tgtctgtgac cgggaagcgg
     1081 atatctggca ttacctgtat tataaagtca gccacggaca gcgtggtgcg tgctgcacag
     1141 aatcgccggc tggaagaggc acccggcaag ctcttcgaac tgccggaagt cctggcaacc
     1201 gcaggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg tcaggcccgg
     1261 atgttcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg ccaggcgctc
     1321 ccgctcacgt atgtctgttg ccgggagcag gcagaggacg gtgcctgctg gcatctgctg
     1381 acgtcaggaa aagcggcgag tgccgccgat gcacgacgta ttgtcagcca ttacgagcga
     1441 cgctggctga cagaggaata ccacaaggcg tggaaaagtg gtggtacatg gaatcgctgc
     1501 gaatgcagac ccgggataac cttgagcgca tggtggttat ccaggcgttt atagcggtac
     1561 gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac acaaaatgac agctgtgaga
     1621 aaatactgac gccgacagag tggaaactac tgtgggttaa gctggaagga aagccgcttc
     1681 cggtgcaggc accgaccctg aaatgggcct ggggcgatgg catgacagca aacgcacagg
     1741 tcgtcccggt tggggcgtca tgtgggatgg ctggtcagac ttcaggatat ggttgagggt
     1801 tacctggtga tgaagtctct tgatcaggag ctctgatcaa gagacagcag cttagggggg
//
LOCUS       DAJTRQ010000254         1787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000254 DAJTRQ010000000
VERSION     DAJTRQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.259"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..606
                     /locus_tag="OO332_004158"
     CDS             <1..606
                     /locus_tag="OO332_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5944377.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            638..1246
                     /locus_tag="OO332_004159"
     CDS             638..1246
                     /locus_tag="OO332_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944378.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
BASE COUNT          510 a          372 c          448 g          457 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     1741 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttac
//
LOCUS       DAJTRQ010000255         1712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000255 DAJTRQ010000000
VERSION     DAJTRQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.260"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..241)
                     /locus_tag="OO332_004160"
     CDS             complement(<1..241)
                     /locus_tag="OO332_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944379.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYT"
     gene            complement(903..>1712)
                     /locus_tag="OO332_004161"
     CDS             complement(903..>1712)
                     /locus_tag="OO332_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944380.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVTA
                     PSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMVD
                     VLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKAL
                     SRLAAKQGFGGEDAIVDMLGTDVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITVV
                     LSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          493 a          384 c          417 g          418 t
ORIGIN      
        1 tggtatagtg actctttatt tcatttttta atttggctga gtgaatcaga aaacgataat
       61 taggatactt atctggattt aacgataaat aacttttatc tgttactgcc cccctggaat
      121 taagaatgtt aaatacagat tcgcttgtca cttctgcctc tgtcgcctgg catgtgccgc
      181 acgaaaatat accagcgagc agaagagtta atgcagattt ccctttcata aaataatcca
      241 tcaatcgttt gttaaaagcg tagataacca cttattcaac aacttgaaaa taaggtattt
      301 tacctgggtt gttacaaaag gattgcattg cgtaaacgct ttttatttac aaaaaaatgg
      361 ggaagtgtta cagcgattgc gaaaagcctg aagtgattga attaagataa tatagaaaat
      421 cagatacagc aaaaatggca gattgatcag gtgaagataa attggagcgg gcgaagggag
      481 ccgaggacaa ctagctaact tcttgtttct taaagactga tgaatccagt ggttacagtg
      541 atggttacac atggagaggc taaaggtcaa gacttgaagt acgtaacatt cccacaaaag
      601 gaatagaaca cagtcacgga ctacccggcc ctttggtctg actgagggtc gggctacggg
      661 gtaaatcgtc aggcttattg tattagatgg tctctcacat ttttcagtca aaaactcatc
      721 gtactttagg ccagaccatc agcatgacta tcagagccag ctttagcagg aagaccacga
      781 gatagacttt gtgtaggtcc attaggtcct cctctttccg tacccatgaa ctagattact
      841 agtacaaaat aaaggccaga catccagtta cggaagtctg accaataagt cgttattgtt
      901 agttagcttg tcttagtaaa gctcaggcgt ttgactacag gcaggacagc agcagcgctg
      961 ccagtcccca tcttaacagt cgttgcagcc tcagcgacca cgaagacctt accgagaatc
     1021 tcaaggtcgt gagactgggt gttgccatct cgggataaga ccacagtaat attctcgcca
     1081 tcgtgacgga agcaaccgaa gctcagagcc gtaaagcgct tagccatcat gaagacgcca
     1141 cggtctgcac cgttgtagta ggcgacgtca gtccccagca tatcgacgat agcgtcttca
     1201 ccaccgaagc cctgcttagc agccagacgg gagagagcct tgaggtgtga ctcatgggcg
     1261 ataatgccgt agtcagaaac ctcagaaccg aaagaagact ccgtgttctc gtacagggca
     1321 tccatgatag cgtcagcctt agcggtcagg ctaccagtca gggccggaag ctctacggtc
     1381 ttcagcagct tagaggactc cagaacgtca accatcttag agttaatggt acggaccacg
     1441 ttatcgcaga tgttcagagc atcctgctgg agcgtccccc gagtgaaacc agtagaggac
     1501 ttctggtccc agagctttgt ctgatgagac acgaggatag cctgttcaat cggctcaacg
     1561 gtgatttcag taccagacgg agcggtcacg acttcaggtc gcttaccacc acggacagga
     1621 acgaccacag agcggtcagc cagagtgtga gcaatgattg gcgtcggtaa gctgttacgg
     1681 gtcggcagga cttctacagg gaacggaatg tt
//
LOCUS       DAJTRQ010000256         1683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000256 DAJTRQ010000000
VERSION     DAJTRQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..444
                     /locus_tag="OO332_004162"
     CDS             <1..444
                     /locus_tag="OO332_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5944381.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OO332_004163"
     CDS             445..810
                     /locus_tag="OO332_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5944382.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..1523
                     /locus_tag="OO332_004164"
                     /pseudo
     CDS             807..1523
                     /locus_tag="OO332_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
BASE COUNT          480 a          350 c          461 g          392 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gaacatcata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggttg
     1321 gtggggaagg ttatgaatac caggagctgg catttactgg cgggagtgac aactaaaaac
     1381 tggtccaaaa catttactcg tcactggatt gcaatgaaac acatttttca ccgactggat
     1441 agtgaggctt tattgtttgt aatgagaaca cgttcaaaaa aaagacggca ttttcaaagc
     1501 aaagtgttgc aaaagtagat tgaaaggcat atattcatgc aaatctgata ttttgccgat
     1561 tttttacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt tgtgccactt
     1621 atctcggata gacatggtga atgcgctggt ggaggagata agggtgattt ttgaatgctt
     1681 gca
//
LOCUS       DAJTRQ010000257         1625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000257 DAJTRQ010000000
VERSION     DAJTRQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..715)
                     /locus_tag="OO332_004165"
     CDS             complement(<1..715)
                     /locus_tag="OO332_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5944383.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGP"
     gene            complement(1031..>1625)
                     /locus_tag="OO332_004166"
     CDS             complement(1031..>1625)
                     /locus_tag="OO332_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5944384.1"
                     /translation="WWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLT
                     HVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYL
                     LHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFS
                     NTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          469 a          301 c          348 g          507 t
ORIGIN      
        1 gtgggccgtg gtagtccggt tgggcggttt gttgtaacaa agtttgcatt attctgggag
       61 atagttgatt ttctctaaga ttgacaacgc tatcagaact gaggccaagt atgctttccg
      121 gaattgccgt gatccggttc tgattaagcc agaatattct tataatatga tcatctcttt
      181 ctggaaactc tggtattact tccaaagcat ttctggctgc atcaagtaat tccaatgaca
      241 ttggtaacct cggaagtaca gacaggtgat tgtcacttac attaatatat tctagtgacg
      301 caggtaattc aggaagtgcg aacaaatggt tatcactcac atttatatat tccagcgaca
      361 ctggtaattc aggaagtgca gataattgat tactgcttgc attcagctct ttcaatgccc
      421 ttggtagctc ggggagcatt gatagttggt tattgcttac attaatttca tcaagattgt
      481 caggcaatcg tggtagagat ctgagaccta aacaagataa gtccaacgat gtttcactgt
      541 tctccagaca taattggagc cgggtaaaag cagtttccct gttttctccg gaaatgctgt
      601 ttttagtcca ttcaacccat tcggagagat aattattgtg aacattgtcg attgatgtag
      661 ttctgtaaaa agagacgttt ccagtggata gggggggatt atttacagga agcataataa
      721 cctcgcagaa ggatatcctg ataaaatgtg atactgggga ataataagct gaaataaaat
      781 gtttcaggat tgaaatttat tgttttcatt ttaaggaagt gatgttgatt taactgtaaa
      841 aaaacgatac tattttctta gatggtaaat gtctcgcctg tgctatattt tgtttttcgg
      901 ttagttaaat atcgtacgtt catatttgaa cgttctgccg gaatgcatta tcaatagagg
      961 taaagtcgca accccaaatc gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg
     1021 cggttggatg ctattttttc cctgaagcgg aaaacattac tacagtacct tgaaccttgg
     1081 ttttaacatt ctcgaaatgc tctgagagta tatgtgttaa gccttcttcg gaatcttttg
     1141 tgtttgaaaa gatgcctttc tgattgtaaa tgcgcatcag tttttgaccg aagctattgt
     1201 gcacaactcc atcgccaaga attgtggctc cgtatagagt tccatcatca gttaaggcct
     1261 gcgccgcatt gcgtattaca cagctttttg tagatatatt tccaggcagg cagtgaagaa
     1321 ggtaaaacat ggaaatggaa tcaaattgac catgtaacgc cgcaggataa ggttcaaaaa
     1381 catcatggct aattttatgt ttaatttttg attccccagc ccttgtagat gccgcgttca
     1441 ggctagcttc gttcaaatcc attaaagata tcagactact ctcaggtacg tgagtaaggt
     1501 aaaacccagt tccaacacca atatccagat ggttgttacc taaatgttcc agaaagtgtg
     1561 gaagaaggtg ttcctttgta ggacatcccc atgcaagccg atttgatact cccaaaaccc
     1621 accag
//
LOCUS       DAJTRQ010000258         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000258 DAJTRQ010000000
VERSION     DAJTRQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..700)
                     /gene="ospC4"
                     /locus_tag="OO332_004167"
                     /pseudo
     CDS             complement(1..700)
                     /gene="ospC4"
                     /locus_tag="OO332_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
     gene            complement(1067..1600)
                     /locus_tag="OO332_004168"
                     /pseudo
     CDS             complement(1067..1600)
                     /locus_tag="OO332_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          455 a          290 c          338 g          517 t
ORIGIN      
        1 atcaaagtgc tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc
       61 attggcgcca aaatctacag tatgatgtgt tttatttagt gggagttttt cttgatgatt
      121 ggaaatttca accccaaaaa acaaaatcgt ggttagagat ttctttaata tcctcagagt
      181 atgtattctt atgtgtttgt atattttcag actgaatttt atcaaaggat attaatatta
      241 agaatattat ttgacagatt agagttactt tgatgtctga agttaaaatt agaattaata
      301 ataatagaaa gtaagtgacg ttcgtctgaa gaaagaggat gaactttttg tattatctca
      361 gagatatagt gtctggacac cttatcgaga tgctcaagtg accgaaatga tgaccgtgct
      421 ccagatggcg cttcaataga aagatttagt gatttgaaac tggtcccttg agaaaacatt
      481 ttgctataac tttgcgctgc tatagacttc cttaaaaagt ttttcacagt attagcacag
      541 tgatctaaat gtttttcact tatgccagaa gaagatgagt ttgttacgcg taatatattt
      601 tttgtaatgc ttttgttgct ttattcggat tcgtttttcc atcaacaaga tcaatattat
      661 tctgaacatt aatatgattt actgcttcag gtattttcat aaaatattcc ccattatgtg
      721 cgtttttatc tatggttatt tagaatatat tatctaactt aatattttct tgtgtgtatt
      781 ataacgaaat gaacatattt taatatgttg tacaattttt actctgaaat cccctgaaat
      841 taatcacacc tcctttactg aaaggaatta ttgcaacggt attatttgtt tttggatata
      901 tatcttgcgc cttgttatgt agttaatcct cagaatatga tagataaaat agagacacag
      961 agcagagcgt gttctaacat catgataaca taaaataatt agtaacaata aatgaaagaa
     1021 tcatgggagc tcaacaaaaa gagagctaaa tagctgtgtg gaatagtaga tcactttggc
     1081 ggtataatgg cagatggtct gcatactttc cggtaacaac atgggccaga agccccgctc
     1141 cggcataact gcgtgcaatg ggttttgaag gcaccggtgc ctgcacgata tggtcgcacc
     1201 ggcgacaggc cagtttaggg cgttgttttt cgataacctt aaaggcgctg ctgataagct
     1261 ccagctgctc tgacacatca catcccagtg gactgagttc accaccacag gccggacagc
     1321 attcctcttc cggccggatg acccgggggg cacggggaag tgaggctggt aacggtttac
     1381 gggctgaaga ctggcgcagg gaagatggca gtaccgggtc atattgctca cccagtgttt
     1441 ccgccatttc ctcctgaagt gcgctgattc gctcctgtgc ttcctgtatc cgccgttcgg
     1501 tttttgcgcg aagtttttct gagcttttac cgaactgcat acgctgcagt ttcgcgacca
     1561 gcgccttcag tcggttgatt tcggaaggcc tggacaactg
//
LOCUS       DAJTRQ010000259         1535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000259 DAJTRQ010000000
VERSION     DAJTRQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..278
                     /locus_tag="OO332_004169"
     CDS             <1..278
                     /locus_tag="OO332_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944385.1"
                     /translation="FSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVV
                     GGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            380..>1535
                     /gene="amn"
                     /locus_tag="OO332_004170"
     CDS             380..>1535
                     /gene="amn"
                     /locus_tag="OO332_004170"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5944386.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWEL"
BASE COUNT          366 a          390 c          362 g          417 t
ORIGIN      
        1 tcttctccat aatgctggca aacattgcgc atgacatggt ggtgtgtgtg caacaaccga
       61 tgtttaccga aatgtttggt gccagttatc gctacagtgg tgctggagtc ggttatcagg
      121 ttgccagtgt ggttggcggt ggatttacac cttttattgc cgctgcactc atcacttact
      181 ttgccgggaa ctggcatagc atcgccattt atttgctggc tggatgcctg atttctgcaa
      241 tgaccgcttt attgatgaaa gacaatcaac gctcgtgata gcctgccgaa gatcatccga
      301 tcttcgcctt atacttttgt ttcacattcc tgtgacatac tatcggatgt gcggtaattg
      361 tatggaacag gagacacaca tgaataataa gggctccggt ctgaccccag ctcaggcact
      421 ggataaactc gacgcgctgt atgagcaatc tgtagttgct ttacgcaacg ccattggcaa
      481 atatattaca attggcgaat tacctgatga aaacgcccgt aaacaaggtc tttttgtcta
      541 tccatcactg accgtaacct gggacggtag cacaacaaat ccccccaaaa cgcgcgcatt
      601 tggtcgtttt actcacgcag gcagctacac caccacgatt actcgcccta ctctctttcg
      661 ttcgtatctg aatgagcaac taacgttgct ttatcaggat tatggtgcgc atatctctgt
      721 gcaaccctcg caacatgaaa tcccttatcc ttatgtcatc gatggctctg aattgacact
      781 tgatcgctca atgagcgctg atttaactcg ctacttcccg acaacagaac tggcgcaaat
      841 cggcgatgaa actgcagacg gcatttatca tccaacggaa ttctccccgc tatcgcattt
      901 tgatgcgcgc cgcgtcgatt tttccctcgc acggttgcgc cattataccg gtacgccagt
      961 agaacatttt caaccgttcg tcttgtttac caactacaca cgttatgtgg atgaattcgt
     1021 tcgttgggga tgcagccaga tcctcgatcc tgatagtccc tacattgccc tttcttgtgc
     1081 tggcgggaac tggatcactg ccgaaaccga agcgccagaa gaagccattt ccgaccttgc
     1141 atggaaaaaa caccagatgc cagcatggca tttaattacc gctgatggtc agggtattac
     1201 tctggtgaat attggcgtgg gaccgtccaa tgctaaaacc atctgcgatc atctggcagt
     1261 gctacgcccg gatgtctggt tgatgattgg tcactgtggc ggattacgtg aaagccaggc
     1321 cattggagat tatgtccttg cacacgctta tttacgcgat gaccacgttc ttgatgcggt
     1381 tctgccgccc gatataccta ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc
     1441 taccaagtta gtcagcggca ggcctggtga ggaagtcaaa cagcggctgc gtacaggtac
     1501 tgtggtaacc acagatgaca ggaactggga gttac
//
LOCUS       DAJTRQ010000260         1525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000260 DAJTRQ010000000
VERSION     DAJTRQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..849
                     /locus_tag="OO332_004171"
     CDS             <1..849
                     /locus_tag="OO332_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5944387.1"
                     /translation="PRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAIENGLIAA
                     TSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTFLNAAGTK
                     TGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADKALLARIE
                     SIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAITGAIAIS
                     SSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIRTTRKIFS
                     GEVYLP"
     gene            925..>1525
                     /locus_tag="OO332_004172"
     CDS             925..>1525
                     /locus_tag="OO332_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCR5944388.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAG"
BASE COUNT          363 a          350 c          391 g          421 t
ORIGIN      
        1 ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca acgtgtcgat
       61 accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat tgaaaatggt
      121 ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa tacgggtacg
      181 ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg tagcgccaga
      241 attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa tgccgctgga
      301 acgaaaaccg gaaaagtttt cccgactgat aatcagattg attattttga cgatgtcccg
      361 gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata tctgggaaaa
      421 acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc ccgcattgaa
      481 tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa tatggttatc
      541 cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt gcgttatttt
      601 atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat ttccagtagt
      661 tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata tggcaatatc
      721 aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg tcaggatgcc
      781 acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg tgaagtttat
      841 cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt ctgtatgagt
      901 catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct aattctgata
      961 ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag cgaactggcg
     1021 tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa gcctttcccg
     1081 gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg taacttatcc
     1141 gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac tacctggctg
     1201 gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg taaacgtatt
     1261 gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta cgttacggta
     1321 ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc gggcggcatt
     1381 gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga aaaaagtccg
     1441 cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac caccagctat
     1501 atgttcttta ccgcaatggc gggga
//
LOCUS       DAJTRQ010000261         1515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000261 DAJTRQ010000000
VERSION     DAJTRQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(71..538)
                     /locus_tag="OO332_004173"
     CDS             complement(71..538)
                     /locus_tag="OO332_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5944389.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(604..843)
                     /locus_tag="OO332_004174"
     CDS             complement(604..843)
                     /locus_tag="OO332_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5944390.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(978..>1515)
                     /locus_tag="OO332_004175"
     CDS             complement(978..>1515)
                     /locus_tag="OO332_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5944391.1"
                     /translation="TTSTGNVINLSENGYRFLLLLLQGESDKQHIINEVWREQKGSVS
                     DSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRYLGKVEKHVCENERDSDEDSSSYT
                     NDSEAREVNTDANIPENDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAV
                     CWLTTLVFLVLLFLNQDK"
BASE COUNT          484 a          334 c          269 g          428 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaagcat ccgatgattc atttatgctg
       61 tcctaaaaaa ttaacaaaac aatgggaaaa caggatatgt aagcagaaaa gccaatgaaa
      121 ttggtacagc ataaggcaca ccgttttcac gaattgattt tcgaaaaaaa agaagacctg
      181 ccagaacaac cagagcgccg caaaaggtca ttgcgacgag aaaattagcc agttcatgtt
      241 catcaatagc taacgaaaga acagttaaca gcttgacatc accgccgcca attatccctg
      301 cggaaaaaag aaagaagccc acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt
      361 gatgctgtaa aatgagtgcc aaaaaaataa gcacggttaa caccagtaaa taacgatggc
      421 taataattct gagtaatata tctccccatg aaataaaaat aagaaagagc gcaacaaata
      481 aaagatcgac tcttagtaac catattgctg ccattgaaag actccatgta ccatacaagg
      541 ttgctgccag acatattcct ttacgtctga cagcctctag agagtttttt tattttttat
      601 aagttacgga ttactcgttc cggaaatcgt tacctgttga attgctgcag cgatagcgtc
      661 gaatgcatct ttaattgctc ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag
      721 cagagttgcc atagcaacac caatcaatgc atattcgatt gcagtaacac cacgttcatc
      781 cttcgcgaaa cgcgcaaagc actgtttagt tttaccatag tacttagtaa taaaagtatt
      841 catgttttcc tcgtatatta ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc
      901 ggtatcccat atttttggga tgtggctata attatccatt cccgctattt agtcttcaaa
      961 aaaaattaca tttaatatca tttatcttga ttaagaaata gtaagactaa aaaaacaagc
     1021 gtcgtcagcc agcacactgc taacacggta agaacgctaa taaaaacact ccactgcttc
     1081 gttctatacc attcaatatt tttgtcagta tgttttattg ggagcagctc ttcagtagag
     1141 aattcctggc tgttatcatt ctcaggaatg tttgcatcag tgtttacttc cctggcctcg
     1201 ctatcgttgg tatagctact actgtcttca tcactgtcac gctcattctc acagacatgt
     1261 ttctcgacct ttcccagata tctgacgcct tttcgcggaa tggttttaat tagagaactc
     1321 gacaaacctg cgacgtctaa tgcctttcta agcatgtaaa tctgcccata ataactgctg
     1381 tcgcttaccg aacctttctg ctcccgccaa acctcattga taatatgttg cttatcggat
     1441 tctccctgta gtaaaaggag taaaaatcga tagccgtttt cagacagatt gattacattg
     1501 cctgtactgg tggtg
//
LOCUS       DAJTRQ010000262         1512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000262 DAJTRQ010000000
VERSION     DAJTRQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..1071)
                     /locus_tag="OO332_004176"
     CDS             complement(<1..1071)
                     /locus_tag="OO332_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5944392.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          454 a          242 c          326 g          490 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatggaagg
     1501 agatcaccca at
//
LOCUS       DAJTRQ010000263         1499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000263 DAJTRQ010000000
VERSION     DAJTRQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            425..1174
                     /locus_tag="OO332_004177"
     CDS             425..1174
                     /locus_tag="OO332_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5944393.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1344..>1499)
                     /locus_tag="OO332_004178"
     CDS             complement(1344..>1499)
                     /locus_tag="OO332_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944394.1"
                     /translation="DAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRL
                     FYQRLRN"
BASE COUNT          413 a          318 c          311 g          457 t
ORIGIN      
        1 gcaggtgggc gctggctcct gagcaccata aacagggctt tacccgtaaa tattggctcc
       61 ttctgatgcc attctatttc aggaaggagt gtccgttaaa ctcaggctac ctcaaaaagt
      121 tatcggtgcg tttatcgaaa aacatatgtt ctactaattg gagtcattgt ctgtaaaagc
      181 aactcaaaaa tacaagttgc ttattcccct tgttggtaag aacttaattc taactgtttg
      241 ttctggatgt tttaaacgtc ctccttttta tgcttgttca taacggatat ttaataggta
      301 acctttgttt tagcatcttc tgatttattt aaattatgtg atagcgcaca caattttatt
      361 atccagtact ttatactctt aataaaataa ttataaatat ttatcctcta taaggagatg
      421 ttatatgaaa agacagcttt ttactcttag tattgtcggg gtattttctt taaatacctt
      481 tgcatcattc cctccgggaa atgatgtgac aacaaaacct gacctttact acctgacaaa
      541 cgataatgct attgacagtc tggcattatt accgcctcca ccacaaatcg gaagtattgc
      601 cttcctgaat gaccaggcca tgtatgaaaa ggggcgttta ttgcgaaata ctgaaagagg
      661 gaagctggca gctgaggatg ccaatctcag tagtggtggc gtggcaaatg tattttctgc
      721 tgcttttggt tctccgataa cggctaaaga ttcaccagaa ttgcataaat tactgacaaa
      781 tatgattgag gatgccggag acctggcaac gcgttcagca aaagaatatt atatgcgtat
      841 tcgacctttt gctttttatg gtgtttctac gtgtaataca aaagaacagg atacattatc
      901 cagaaatggc tcttatccat caggtcatac atcgattggt tgggcaacag cacttgtatt
      961 gtccgaaata aatccggcac gccaggatac cattctgaaa cggggttatg aactggggga
     1021 cagcagggtt atttgcggtt atcactggca aagtgatgtt gacgcagcac gtattgttgg
     1081 ctctgctatt gtagcaacac tgcactcaaa tcctgtgttc caggcacaat tacaaaaagc
     1141 gaaagatgaa ttcgctaaca atcagaaaaa ataatgcttt tagccagtac gccttataac
     1201 cccgaccttc agggctaatg ccagtcagtt aagcaactga ctggctcttt ttcagggctg
     1261 tggggtattt ccagggcctc tcctttacca ctctcgggaa ggccctttcc cttcttgtcg
     1321 gtaatttcac taggctgtgt cccttaatta cgtaagcgtt gataaaagag tcggatgcag
     1381 cccagtttca ccatcgccag gtaatttctc gccgttttgt cgtaacgcgt ggcgatgcgg
     1441 cgatattctt tcagccgccc aaagcaccgt tcaacgacat tacgattgca gtacgcatc
//
LOCUS       DAJTRQ010000264         1480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000264 DAJTRQ010000000
VERSION     DAJTRQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..800)
                     /gene="ipaH7.8"
                     /locus_tag="OO332_004179"
     CDS             complement(<1..800)
                     /gene="ipaH7.8"
                     /locus_tag="OO332_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5944395.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQ"
     gene            1129..1308
                     /locus_tag="OO332_004180"
                     /pseudo
     CDS             1129..1308
                     /locus_tag="OO332_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1325..1480)
                     /locus_tag="OO332_004181"
                     /pseudo
     CDS             complement(1325..1480)
                     /locus_tag="OO332_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          459 a          226 c          322 g          473 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc tgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggattgcag aacgcgagtc gacaatggat tccctgaaat attaatcctg
      121 taacttgaat ctaattgtga aaaactctcc ggaagagtgg ttatctgatt aaaatgaaaa
      181 taatattcct tttgtctgat attgtttcta tcagaaaatt gaggaaaagt aaccagatta
      241 ttgcgctgag cacagagaat ttccagttta tccggtaagt cgggtaatgt tgtcagcctg
      301 ttataataaa catgtaaaaa tttcaatttt tcgggaagag ccggtaagtt atatagcctg
      361 ttattttcaa caaataatga tgttaaatgg tttggcaaca caggaaggtt ttccagttga
      421 ttagaacgaa cacttaaagt tgttaatgac tcaggaagtt ctggtatcac agaaagcctg
      481 ttattatcag cattaagttc tttgagaaat gcaggcagct ttggcaatga tgttaattta
      541 ttattttcta caatcaaagc agaaatatgc tttggtaatt cgggaagaga cgtcaaattt
      601 aggtctgata aatttaatac tgcttcttga ttctgaaagc actgggagag tctgttaaaa
      661 gcaatgcctc gctcttcccc gggagaagag ttcttttccc attcagtcag gattctcaga
      721 taatgttcat tactggttga gtttgagtta atagaggggg agcaagaaac tgatgagtgt
      781 gtattattta cagagaacat aatgctaggt caaccttata tttgtgagaa taaatattta
      841 aaattgtata tttatacaaa tttcacttac catttccgat attaagtttt aatagtttcc
      901 attttacaca taacagaagt tatgttgtaa tttatttatg gaggttttct atggaattct
      961 tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg agcaaggaaa
     1021 tgtaatccga gtaagtatat ttgttttaca tcatatttta gtcaagctca tttattccta
     1081 ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat acgagggagg
     1141 ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat gaagtccttg
     1201 tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat ggtaaaggcg
     1261 ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttaccgggt agtacttcca
     1321 atcattagaa tatgtgcttt tcaatgaagg aaccgatgac tttttcgtgg atttccacgg
     1381 aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat gcgggggcgg agggtcaggt
     1441 tgttacgttc atacgttgcg tgaagatttt gccggtcagg
//
LOCUS       DAJTRQ010000265         1469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000265 DAJTRQ010000000
VERSION     DAJTRQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(755..1231)
                     /locus_tag="OO332_004182"
     CDS             complement(755..1231)
                     /locus_tag="OO332_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5944396.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          336 a          389 c          362 g          382 t
ORIGIN      
        1 gattttgccc gatttcgcgc tctccacgtt caacagctcg atcatgtttt tgccgtccgt
       61 tcaccgttaa aacgcctcag aaatcgggac aggatgtggg aatgtcggac cgcgcacgcg
      121 cactcagaca ttccccacct ggtcagggct tcaccccgac accctgcatc cgtgcgtaag
      181 tgctcaatgt aacttccggt tctcttccgc ttcctcgtcg attcgctcgc tacgctcggt
      241 catcgacttg cggcgagcgg gatcacctct cgcatgttgt cggtgatccc gttgttgttc
      301 tttcggggat gagtcgtcag atagcccttc aaacacgctc agaagctctc agacgcacga
      361 aacgggttta aatgcccgcc agggcatgga aggcgcgaaa aacgcttaca gaggctctca
      421 ggaggtcagg cgcgccgata cccctaacca ggggctttca ggtcgctagg aaccgcgtca
      481 gctccctgca aagcctattt gcacggtaca ggcagctatt cggctggttg gtggtcgtag
      541 accgtcggtg ttacagcgat gggggtctgg ggttctcccc agacggtttc cggttgcgct
      601 caaggcgctg ccggaaccgt ggcgacgcgg tcgttcttgg gatcggggag caaaaacacg
      661 caagggattt tgaggcggca aaagagcaat gcaggataga caatcatggt caaaacgtta
      721 acacgtagcc cctgattgtc cgcctgccgc tggctcaatt tgaaaacatg gccatgattc
      781 ggcgctgaat atcaccgtct ttgttgacca gtgttttagt gacaaacgct gcatggtttt
      841 tcgtagtgtt gtatcccttc cagacttcat gtggatcgag ggcatacgca caaaccttac
      901 cagcaacggg gattctaact aatgcatttt ttgattctaa gtaattgagc caccgaataa
      961 ttgtgcttct gctaactcca agtttttcag ctaggaattg ctgatccgca acgacagcgc
     1021 cacacgtagg atcgatatgc tccgctaaaa atgagtacag cttcagtgct gatatgcctt
     1081 ggctatcctt cagcagctct cgaacgcgtt cccaaccttt tggggatacc tgtgtaaacc
     1141 ttgaattctt ctttgcttct tcgagatcct cggtgttttt ctttgcctca tctgaaagtt
     1201 gtttcagtcg tttttctgaa tcaataaaca tgattttttc cctctgaaaa tcccatgcac
     1261 taacgttggt tcaaatctaa gccatcatga cacatctgtg caacaccttg ttgcgtgttg
     1321 tcgcatccag gatgcgacaa cgttgtgtca ccctggatgc gacaggtaaa caaaaaacac
     1381 actaaaaaaa caacaactta caaatcggcg taaaagatct attctaggca agggtgttgc
     1441 gcgcaacgca acgccctttt ggggtgtcg
//
LOCUS       DAJTRQ010000266         1431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000266 DAJTRQ010000000
VERSION     DAJTRQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            7..159
                     /locus_tag="OO332_004183"
     CDS             7..159
                     /locus_tag="OO332_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944397.1"
                     /translation="MGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEI
                     FQKGAA"
     gene            156..1226
                     /locus_tag="OO332_004184"
                     /pseudo
     CDS             156..1226
                     /locus_tag="OO332_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
BASE COUNT          337 a          329 c          416 g          349 t
ORIGIN      
        1 aactggctgg gggactggtg cagtgaagag cagcatccgg tgatctggtc aaccacgcag
       61 gctgcagaac acttaccggt tcgcatgccc cgtctctgct ctgcagaacg cctctctgaa
      121 agtatggtcg atgaaatatt tcagaagggg gctgcatgag tatagaaata aaaacagggc
      181 tggtgctgtc cggcggtggg gcggtgggcg cttatcaggc gggagtggtt aaggcactgg
      241 cagagtgtgg tacacagatc agcatggttt caggggccag cattggcgca ttcaatggtg
      301 ccattatcgc ggcctctccc gatctgtcag aagctgccgt acgcctggag gcgctctggg
      361 atcatcttgg gaataatcag gtgctgtcgg taaaccgatc ggtttacttt tcattgctga
      421 aaaaattgtt ccaggcaatg aacctctgcc agatccccgg acgtgcagga gcactgctta
      481 cgacacttct tcgccatata tcgataatca acggcttcga caatccgatg actcagccgt
      541 tgttgtcaga tgggcccctg acagcgttga tggatcatta tcttgataca gatgctctgg
      601 caggcgggct accgctgtat gtgtcgctgt accccacaga agggggcatg caggatatta
      661 ttgactgcat tcgtgctgaa ctgggttccg gaaccacgaa aaacgccgtt tttcagcata
      721 tccagagtct gccccgcgga cagcagaaag aggctctgct tgcgtcagcc gcactgcccc
      781 tgttgttccg tccccgtgag gttcagggga caatgttcgg tgatggtggt atgggaggat
      841 ggcgaaatat gcagggaaat acccctgtaa cgcctctggt cgatgccgga tgcaatatgg
      901 tgattgtgtc gcatctgagt gacggttctt tatgggatcg ccaggctttt ccggacacca
      961 caatccttga gatccgtccc cggaaaaggc tgaaatatgc aggtgatggt ggcaacagtg
     1021 gcggtctgct cagtttcacg tcggcacata ccgacgcctg gcgtcagtag ggctacgagg
     1081 acacgatgct gacgatggag catatccgga aaccgctggc agcacgtcag gcactgtccc
     1141 ggtcagaaac ggtgttgcag aaaagtctgg agataacgga agaggcagat ttggcactga
     1201 gaagcgcgat ggcccggatt aaataaagac gccccaaaga aacgccaccg cctgtggtgg
     1261 tgtttttcct gtctggtgtt gttgaaaata ttttcagata tatattacct gggtgagatt
     1321 taagattaac gcatttatta atgtcataag gagagttatc atgaaattca tcgtaaaagc
     1381 acttaggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg a
//
LOCUS       DAJTRQ010000267         1374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000267 DAJTRQ010000000
VERSION     DAJTRQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.273"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..676)
                     /gene="corA"
                     /locus_tag="OO332_004185"
     CDS             complement(<1..676)
                     /gene="corA"
                     /locus_tag="OO332_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5944398.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIES"
     gene            1046..>1374
                     /locus_tag="OO332_004186"
     CDS             1046..>1374
                     /locus_tag="OO332_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944399.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQR"
BASE COUNT          351 a          356 c          347 g          320 t
ORIGIN      
        1 gggattcgat atcgcgcagg atttcacgcg cctgctccag ttgcccaccc ggtaaacgcg
       61 ctttacgcac caggaagttg agagcgcgct gggtatccat cagacacagg cgaactttcc
      121 agccgatatc ttccagttcc gccagcgtgg agagcgcctc gtcgtactca tcgccctgat
      181 gcccttccat aatcacccgg ctcaactgct ccaggtcgct atagatattt tcaatttcat
      241 ctgccaactg ttcgattttg gtttcgaaca gatccagcag caactcgtag gcgttaccgt
      301 ctaccatcga ctggctacgg gcacgcatac gatacagacg aaaagcgggc agttcacgct
      361 cacgcagagt aaacagacga ccatcacgga tggtaaatgc cacagtggag ttaccggcgt
      421 gatcttccgc atcttcaaag aagaagaagg agtgaatatg caggccgtcg tcgtcttcaa
      481 agaaacgtgc cgatgcttcg atgtcttcca gttccgggcg ggttgccagg ctctggccaa
      541 gttcagattg tacgcgcagt cgctcgtcgt cgtccggttc gacaagatca atccatactg
      601 catttacaag gggttgtgac tcttcgactt ccagccgggt cagtcggtta ttttccagtt
      661 gaaatgcgct cagcatgacc gggactccca atgcttaaaa tatcggacag ttcggtgggc
      721 acacagaaac aaattgggtt tcagaccatt aaacagcctg actcagcgcg acgggaaaaa
      781 ttgaggtcgc tgacaaccgc taaggctatc agcaaaaagg gatagcctta ggagttgatc
      841 ctggatgaca ggatagtgag ccagtatcta ctgggtgtgt ccaaggcgaa tgtcctctta
      901 gagtgatcgt gcgcgcatgt tacgccagca aaattttgcc gtcaacacgc aacagaacac
      961 cacagaacaa tagttgaact tcgcgctttt tggtactcgt caaggtcgct aacaggataa
     1021 aaaaatccca catccaggac ctataatggc gtatcggcta ctcattggta aaggaatgat
     1081 caccttgaat ctcaaacgga tttttctcac cctcacctta cttcccttgt ttgctgttgc
     1141 cgctgatgat tgcgcactct cagatccgac gctgaccgta caggcgtata ccgttaatcc
     1201 tcaaacagag cgggtgaaaa tgtactggca aaaagccaat ggcgaagcgt ggggaacgtt
     1261 acatgctctg ctggcggata ttaatagtca gggtcaggtg cagatggcga tgaacggcga
     1321 catctatgat gaaagctatg cgccgctcgg tttgtacatc gaaaacggtc agcg
//
LOCUS       DAJTRQ010000268         1347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000268 DAJTRQ010000000
VERSION     DAJTRQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..264)
                     /locus_tag="OO332_004187"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO332_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO332_004188"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO332_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5944400.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1176..1347)
                     /locus_tag="OO332_004189"
                     /pseudo
     CDS             complement(1176..1347)
                     /locus_tag="OO332_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          393 a          337 c          218 g          399 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg catctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag ggctaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg cataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaac
//
LOCUS       DAJTRQ010000269         1341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000269 DAJTRQ010000000
VERSION     DAJTRQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.275"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            127..996
                     /locus_tag="OO332_004190"
     CDS             127..996
                     /locus_tag="OO332_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5944401.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..>1341)
                     /locus_tag="OO332_004191"
     CDS             complement(1199..>1341)
                     /locus_tag="OO332_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5944402.1"
                     /translation="ANDLRNQAAAMGGNVIYGISSPSQGMLSSFVPTDSQIIGQVYKC
                     PN"
BASE COUNT          391 a          309 c          301 g          340 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tgccgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgctcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct g
//
LOCUS       DAJTRQ010000270         1312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000270 DAJTRQ010000000
VERSION     DAJTRQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..274
                     /locus_tag="OO332_004192"
     CDS             <1..274
                     /locus_tag="OO332_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944403.1"
                     /translation="YLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYK
                     TDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            276..880
                     /locus_tag="OO332_004193"
                     /pseudo
     CDS             276..880
                     /locus_tag="OO332_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            867..>1312
                     /locus_tag="OO332_004194"
     CDS             867..>1312
                     /locus_tag="OO332_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5944404.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLS"
BASE COUNT          304 a          316 c          364 g          328 t
ORIGIN      
        1 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
       61 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      121 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      181 cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      241 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      301 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      361 tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg ataccgcaga
      421 aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc tgacggtaaa
      481 ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg taatggatga
      541 tccctgtccg tttaatcagg caccgtttta caacggcaaa tcggataccc ggactgttga
      601 cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga acattactga
      661 ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa aacgccgggc
      721 ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg cgctctcgtt
      781 ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg gtgtttatgt
      841 ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc cagctggcgg
      901 tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca cagcagacca
      961 gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg acagcggtct
     1021 cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac gatattaccg
     1081 cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt tttgcggggg
     1141 ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca gcggtctggc
     1201 tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg ggctgggtaa
     1261 atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg tc
//
LOCUS       DAJTRQ010000271         1307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000271 DAJTRQ010000000
VERSION     DAJTRQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>1307
                     /locus_tag="OO332_004195"
     CDS             <1..>1307
                     /locus_tag="OO332_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5944405.1"
                     /translation="AVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLM
                     TAFFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTS
                     LARDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSR
                     AIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVT
                     EEGKQDDEEKPQPSGAMTDEQATAETVEPNATEHHQNTQPLDAQSQVNSVDEKYQELR
                     AELHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENE
                     VRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPK
                     KTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYG"
BASE COUNT          389 a          322 c          334 g          262 t
ORIGIN      
        1 gctgttactg ccctagctgt ttcccacaaa accagcctgc tcgatccgct ggaaattcgc
       61 gatctccaca aactggtgcg tgatactgac agagttttcc ctaatccagg caattcaagc
      121 ctggggctga tgactgcttt tttcgaagca tacatggacg cagactacac cgatcgcggt
      181 ctgctgacaa aagagtggat gaaaggaaat cgtgtttcac gcatcacgcg cacggcttcc
      241 ggcgctaatg ctggcggcgg gaacctcacc gatcgcggcg aaggtttcgt tcacgatctg
      301 acgtcactgg cgcgcgacgt agccactggc gtactggctc gttcaatgga cgtggacatt
      361 tataaccttc atccggcaca cgctaaacgt gtcgaggaaa ttatcgctga aaataaaccg
      421 cccttttctg ttttccgcga caaattcatc accatgcctg gcgggatgga ttattcccgc
      481 gccatcgtgg ttgcgtcagt gaaagaagca ccgattggta tcgaggtcat ccccgctcac
      541 gtcactgaat atctgaacaa ggtactgact gaaaccgatc atgccaaccc tgatccggaa
      601 atcgtggata ttgcctgcgg tcgctcctct gccccgatgc cgcagcgagt aacagaagaa
      661 ggaaaacagg acgatgaaga aaaaccacaa ccatctggcg caatgacaga tgaacaggca
      721 acggctgaaa cagtggaacc gaatgcaact gaacatcatc agaacacgca gccgctggat
      781 gctcagtcac aggtaaattc tgttgatgag aaatatcagg aactgcgggc agaactccat
      841 gaagcccgga aaaacattcc gcccaaaaat cctgtcgatg cagacaaatt actggctgcc
      901 tctcgcggag aatttgttga agggattagc gacccgaatg attcgaaatg gattcccggt
      961 catcatatct cctcaaatga agtcaaaaaa acggaaaatg aagtgcgaca gactgaagat
     1021 aaacagcacc agaacagcga accggaggag ggaacaccct gcaatcagac tggcgaggat
     1081 aactgccctg actgtggcgc ggtgatggga gacgcaactt atcaggaaac ttttgacgaa
     1141 aaaaaaccag atgaagcaca ggaagaagag ccgaagaaaa cggaaaaagc tgatgatttg
     1201 ttaccggaga acgctggcag cgatcagcat aatgatagca ataatgaaac tggcgaagat
     1261 gaactcaact ggaaaaaaca ggtgttgatt gcggcggttt acggttt
//
LOCUS       DAJTRQ010000272         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000272 DAJTRQ010000000
VERSION     DAJTRQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1126
                     /locus_tag="OO332_004196"
     CDS             <1..1126
                     /locus_tag="OO332_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5944406.1"
                     /translation="AVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCREN
                     VQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMF
                     SEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCRWV
                     SAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSP
                     WQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISI
                     DDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGV
                     ESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1134..>1294
                     /locus_tag="OO332_004197"
     CDS             1134..>1294
                     /locus_tag="OO332_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944407.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIA"
BASE COUNT          362 a          256 c          314 g          362 t
ORIGIN      
        1 agcggtgttt ggtgttgatg aacgcaataa aatcgtcagg aaattgtgtc agcgaattgc
       61 cgagaaatat gcgcaatgct gcgatatcgt gacattcaat gccgatctct atttacttct
      121 gtgtcgggaa aatgtacaga catttacccg taaaatagcg atggtaaacg attttgacag
      181 cagctttggc taccgcaatc tgcgcatcca taagtctgcc atttgtgaac ctttgcaggg
      241 ggaaaacgcc tggagttacg cagaaaaact gaaactggcg atttccagta tccgtgacca
      301 tatgttctcc gagtttattt tctgtgatga cgcgaaactc aacgaaatag aagagaatat
      361 ctggattgcg cgtaatattc gccatgcaat ggaaattggc gaactattcc tcgtctatca
      421 accgatcgtt gatattaaca cccgcgccat tctgagcgcg gaggcgttgt gccgttgggt
      481 ttctgcggag cgggggatca tttcaccgct gaagttcatt accattgctg aagatatcgg
      541 gtttatcaat gagctgggtt atcagattat taaaacggca atgggtgaat tcagacattt
      601 tagtcaacgt gcgtcgctga aggatgattt cttactgcat attaatgttt cgccctggca
      661 gttaaacgaa ccgcactttc atgagcgttt taccaccatc atgaaagaaa atggcctgaa
      721 ggcgaacagc ctctgtgttg agatcactga aaccgtgatc gagcgaatta atgaacattt
      781 ttatctcaat attgaacaac tgcgtaaaca aggggtacgg atatcgattg atgactttgg
      841 caccggtttg tcaaacctga aacgttttta tgaaattaat ccagacagca taaaggtgga
      901 ctcgcaattt accggcgata ttttcggtac tgcgggaaaa attgtgcgca ttattttcga
      961 tctggcacgc tataaccgga tcccggtgat tgcggaaggc gtagagagcg aagacgttgc
     1021 gcgcgaatta attaaattag gatgcgttca ggctcagggg tatctgtacc agaaacccat
     1081 gccattctcc gcctgggata aaagtggaaa attagtaaaa gagtagttta cgtatgtcca
     1141 gaatcaataa gttcgtactt acagtcagtc tgctgatttt tatcatgatt tcagcagttg
     1201 cctgcgggat ctacactcaa atggtaaagg aacgggtgta tagcctgaaa cagtccgtta
     1261 ttgatactgc ttttgcggtg gcaaatattg ctga
//
LOCUS       DAJTRQ010000273         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000273 DAJTRQ010000000
VERSION     DAJTRQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..809)
                     /gene="ipaH4.5"
                     /locus_tag="OO332_004198"
     CDS             complement(<1..809)
                     /gene="ipaH4.5"
                     /locus_tag="OO332_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5944408.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLT"
BASE COUNT          363 a          229 c          278 g          407 t
ORIGIN      
        1 gttaatcttt gcagggattg cagaacgtga gtcgacaatg gattccctga aatgttaaca
       61 ttcgtaaaaa tgggcaatgt gattatattt tcgggtaatg aagttagtag gttattggaa
      121 acatccagtg ccaatagttc ttgcggtaat ctggataatc tgcgtagttg attttcacct
      181 accttcaaat attttaagct ctgaggaaat tctggtagtt cctgtagtct gttgccggaa
      241 acaaagagtt cttgtagtct atgaggcaat tcaggcaaga cagtaaggcg attaccttca
      301 atacgtaata ttgttagcgt tgatggaagc tctggtaacg tctccagact attaaaacgt
      361 gccgagagtg attgtaaata tggtggtaat gatggcagac atgacaaatt tcgattgaat
      421 gaaatattaa gcttaatcag ttgagatgga agtgtcggga gtatattcaa gttgttacca
      481 tttacatgaa gttctgtcaa aagcggagaa ttttcaggta gggagattaa ctcattgttg
      541 gaaatattaa gttcacgtat atgcaaaggg agtggtggta aagaacgtag tttcagtaag
      601 cttaaattga gaacaggctc ccgggtatcc agacatattt tcaataatcg aaccgcctgg
      661 atgcgatttt ctgttgttgt tccttcccta gcccagtcat cccagatgcg gtggtaatct
      721 ctggtacact gttcttctac cgataaacga gatatacatg ataagccaca aagggaacga
      781 aaaaaagaat gattgttgat cggtttcatt tattaacccc tcactattca tatcttagtc
      841 tattaatgca aataaaacga gaaagtcttt aaaaaaacaa tcctagagct ttatcggcgt
      901 tttcattccg gctggaggaa tatgttcctg tctttccagt aaccgtcgat tgttatatca
      961 gtctacccat gttaatgtga ccagttctac tttcatccag tttggattga tctttatccc
     1021 ctatattttc aaacaatttt tatcatttaa ctcattactg tctcgatatt cccatagcaa
     1081 acgatctggt tggttatccc ctcaaaaaag tcagttgcac gccgtaaatc cctgccggta
     1141 ttgagcgagg atttacgggt tgttgttaag gtccgtaatc gggtcacagt ttttccggag
     1201 tcaatccggt ctgcggttta tgcttatgcg acgtgattat gaatggtgca gtcgtgagcc
     1261 gttttcagac aatcgca
//
LOCUS       DAJTRQ010000274         1270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000274 DAJTRQ010000000
VERSION     DAJTRQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            66..1259
                     /locus_tag="OO332_004199"
     CDS             66..1259
                     /locus_tag="OO332_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5944409.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          379 a          265 c          290 g          336 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agtaattttg
//
LOCUS       DAJTRQ010000275         1247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000275 DAJTRQ010000000
VERSION     DAJTRQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            210..1034
                     /locus_tag="OO332_004200"
     CDS             210..1034
                     /locus_tag="OO332_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5944410.1"
                     /translation="MVFITIFTSCYVSSKAYKQLSKSTRLYSDNHMWTKWSIYSIHGS
                     NGRDKYTYTKTLIYKKITTHEYVFIAICLIQFISIIMTSSETRVDMVLGANFATLCKI
                     LTTKVFLGLWANGDFLSPLWNHDSICIAITMICLSITIFTFIVSNYPMRSAIVFAILT
                     LTFSLAKPMLSSTSEQWPLLIHGGGRYSVIPTIIWYSVLVYFLNYISHLHFKFLPWML
                     YTTSLISCLYFFNLTPLPDYGWKEQVKKFESLAPGESYSFKFNPAGWTMTLIKKQP"
     gene            complement(1158..>1247)
                     /locus_tag="OO332_004201"
     CDS             complement(1158..>1247)
                     /locus_tag="OO332_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944411.1"
                     /translation="LLAWKKYRVLLMRVDIKNTEWPKKPEENK"
BASE COUNT          374 a          245 c          182 g          446 t
ORIGIN      
        1 cttattgcct ccatatcatt aaattttaat ttaatgtatg cccctttaat ttttaacatc
       61 tccgcaatta ttgttagggc attgcttgta tctttcatac tctctggaag atttagttac
      121 gtaaacataa caccaagaat atttactgct ttgtttatta taataatgcc ggaagtatca
      181 gaagtgcacg ccaatgttac taatgattat tggtatttat cactatattt acttcttgtt
      241 atgttagctc caaagcctac aaacaattat caaaaagcac acgattatat agcgataatc
      301 atatgtggac taagtggtcc atttatagta ttcatggctc caatggtagg gataaatata
      361 cttatacaaa aacgcttatc tacaaaaaaa taaccactca cgaatacgtt ttcattgcca
      421 tttgtttgat tcaatttatt tctattatta tgacatcctc tgagaccaga gttgatatgg
      481 ttcttggagc taatttcgca acactttgca aaatattaac aacaaaggtt tttctgggtt
      541 tatgggctaa tggtgacttt ttatcacctt tatggaatca tgattcaatt tgcattgcaa
      601 ttacaatgat ttgtctttcc atcactattt ttacatttat tgtttccaat taccccatgc
      661 gctcggcaat agttttcgca atactaacat taacgttttc actagcaaaa ccaatgcttt
      721 catctacaag tgagcaatgg ccattattaa ttcatggcgg aggaagatat tctgtcatac
      781 caacaatcat atggtactct gtattggttt acttcttaaa ctatatttcc cacttgcact
      841 ttaaattcct accatggatg ttatatacaa catcgttaat ctcatgttta tattttttca
      901 atcttacccc actcccagat tacgggtgga aagagcaagt taaaaaattt gaaagcttag
      961 caccaggaga atcgtattct ttcaagttca accctgcagg atggacgatg acattaataa
     1021 aaaaacagcc ttaatgttga atggtagtta cttaatgtta ttgatctaag ttttgcatca
     1081 ataacccccc ccgaaactta ggggggggca tctgcagtta taattaacgg cctctttctt
     1141 catccgataa cgccccctta tttattttcc tctggttttt tcggccactc tgtatttttt
     1201 atatcaacac gcatcaatag cacccgatat tttttccatg ccagtaa
//
LOCUS       DAJTRQ010000276         1245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000276 DAJTRQ010000000
VERSION     DAJTRQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(115..324)
                     /locus_tag="OO332_004202"
                     /pseudo
     CDS             complement(115..324)
                     /locus_tag="OO332_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(374..>1245)
                     /locus_tag="OO332_004203"
     CDS             complement(374..>1245)
                     /locus_tag="OO332_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944412.1"
                     /translation="ALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQ
                     VDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSF
                     VYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLF
                     RNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSM
                     ELVPKEAAGAASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFT
                     LMSRERSLEFEKE"
BASE COUNT          395 a          303 c          253 g          294 t
ORIGIN      
        1 gaaaacatag tcttactggg gacatctgac atcacaaatc cccgggtggg gatttgtgta
       61 taagagacag cccatggagg aaggagatat atcgcagctc tgacgactca ctccttattt
      121 acccgggaaa tacttctcca gcaatgcata caggatggca gcggtttcag catccattat
      181 gccgtcataa tttttctgcc ggaaatgaag ctgaaaagcg cggacaagcg ccttatatcc
      241 gtcatccgtc tccacaccag aaatactgta tccataggtc ttcagccgtg acacgatatc
      301 aaccttatcc ggaatgccct gcgattcaaa aaataaatat ttagtcagta attttatgtt
      361 tttcctgaga aaattactct ttttcgaact ccagtgagcg ttcacgactc atcagtgtga
      421 aaatgatggc aaaaacagca gacagtatca gtacgataaa tgtggccccc catcccagat
      481 aatcgacaac aagtcccatt cccagtgtcg caacaattga gccgaagata taggaaaatg
      541 tgcctgacag ccctgatgcg gctccggcag cttctttagg aacaagctcc attgaataaa
      601 gaccaataaa catcaccgga ccatacactg ccagccccaa cagaataagg attgtatagt
      661 ttagccagag atattcattg cctgatggca cgtaccagta cgcgattaat aaaataatgg
      721 taagtgccag aaataaagta tttgttaatg gttttgagtt tctgaacagt ctgacagaca
      781 ggtatccggc aagaagcata ccagccacac caccgatttc aaaaacagaa aaccccccca
      841 ttgatgccag ttttcccata tccttaccac cgacatccag actgtcactt aaaaacttag
      901 gagcccagga aacaataccg tatctcagaa tataaacaaa cgaaccatag atacagcagt
      961 aaatgagttt attatttcgg atgatatgtt ttctgatgat atcaggaatg gagagtctcg
     1021 gaccttgttc acggccttta gccgggtgtg aaacgacgtt acctttccat tcttcaatgg
     1081 gaggaagccc cactgataca ggtctgtcca cctgaataaa gtaaatcagc acactcattg
     1141 ccatacacag tactcctggg aagataaatg cggcccgggc atgctccaga gcactgtcgc
     1201 cgaaaaaacc aaaactgaaa ccaatcagaa cgggggccag agctc
//
LOCUS       DAJTRQ010000277         1238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000277 DAJTRQ010000000
VERSION     DAJTRQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..365
                     /locus_tag="OO332_004204"
                     /pseudo
     CDS             1..365
                     /locus_tag="OO332_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..766
                     /locus_tag="OO332_004205"
                     /pseudo
     CDS             293..766
                     /locus_tag="OO332_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          306 a          307 c          375 g          250 t
ORIGIN      
        1 caaggcaatg tgccgcgtac tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg
       61 gcggacaagg ataaacacac gtcagcagtt ccgtcagcac tgcgacagcg ttgtccgcaa
      121 ggcttttacc cagtccagcg ttacggtgcc ccacgcctga cggatgaact gtgtgctctg
      181 gttaccactt taacgtaaaa accgtggcag ccagtctgca ccgtcaggaa ctgagcgcaa
      241 aagcctccca gaagttcagc ccgatcagtt accgtgcaca cggcctgcct gtatcagaaa
      301 atctgctgac gcaggatttt tacgccagtg gcccgaacca gaagtgggcg ggagacatca
      361 cgtactatta cagctcaccg actgcgggga aacacggggc gccgggttat tgaaggtgac
      421 ctgtccagct acttcgacac agtacatcac cggctgctga tgaaagccgt ctgccgcagg
      481 atcagtgatg cacgttttat gagactgctg tggaaaacgc cgtgttgatg ttggtctctt
      541 ccgggcggtc agtgaaagta tgccacaggg cggtgtgatc tcgccgttgc tgtcgaacat
      601 catactgaat gagttcgacc agtatctgaa taaacgctac ctgagtggaa aagccagaaa
      661 ggatcgctgg tactggaatc acagtatcca gcgaggccga agtactgctg tcaaagaaaa
      721 ctggcaatgg aaacctgccg ttgcttactg ttgctatgcc gatgactgtg tgccacgaag
      781 gcgcgtcctg gggacgtgag tccctgtgca gccatgcagc acaagagatg agggaaggcc
      841 cctcggtgtc ggcgtgcagg cgcaggcacc aaaccatcca ctgctgctgc atttaagaga
      901 tgtggtggtg agcgagatgg gaaaaggtcg gacgtgagcc gatcaggtat ggttcgaaga
      961 gacgaacgaa agtgaaccgc tgatgatgtg tcgaaagcat atagatgatg tcaaaacaat
     1021 ggtggagtcg ttcctgtggg atatgtttac tgcctgcgcg gcatccggca taaaagcggc
     1081 gtgaagtcga ttcaggctct tgtggggaac gtggacctgt caccccgatg tgaagggaga
     1141 agcgcaagtg gaagccccat gagcgccaga gtaccaatgc ggggtacagg gacggagcac
     1201 caagcgattg cgcagtaaac atgcttgaca acttaatt
//
LOCUS       DAJTRQ010000278         1229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000278 DAJTRQ010000000
VERSION     DAJTRQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.284"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..845)
                     /locus_tag="OO332_004206"
     CDS             complement(<1..845)
                     /locus_tag="OO332_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5944413.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIG"
BASE COUNT          382 a          289 c          241 g          317 t
ORIGIN      
        1 ccaatcagct gctcttcgat taactgcttg cagtcccaga tactgttaaa cacttcaccg
       61 accgcaatcg gcgtcacggt atgctggcga atcagacgaa aacattcctg gttctcggca
      121 ggcgtcggat cttccatcca gaacaggcgg taatcctcaa tgctcttccc aaagcgcgcc
      181 gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca gatgttcgtt aaaaccgaac
      241 ttattacgca ccgcttcgaa taacttcggc gtaaaatcga gatacttttc tgaagaccac
      301 aactgctctt ctggccacag gcctttggtt gccggttcat acgccagtcc tttgcccttc
      361 gccaggccat aagtggtttg catacccggt acaccgcact gcacacgaat cgccttaaag
      421 cccatctgct ggtgctttgc atagtcttca aggacttcat caatcgtacg cccggtggtg
      481 tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa gcagttgata caatggcata
      541 tttgccgctt tagctttgat atcccacaac gccatatcaa tggcagaaat ggctgacatc
      601 gtgacggggc cacgtcgcca gtaagcacct ttataaaaga attgccagat atcttcgata
      661 cggctggcat cgcggccaat tagttgtgga caaagatgat ctttcagata agaagcgacc
      721 gacaattcgc gaccatttaa cgtggcatct cctaatccac aaagtccgct ttcagtggtt
      781 attttaagcg taacgaaatt acgccctggg caagtaacaa aaacatctgc agcaataatt
      841 ttcattattg caatctcctg tgttgtaaat agacgtttca aacctaattt tacgcctact
      901 accatacaag agagaaaaag cgattattca accgtgatca cattaaaaac aaaaatttcc
      961 accgaggaga aaaaataccc tcaaaaacca cctgaataat taaccattat tgttaaaaca
     1021 acacgttaaa taatcacata acaaactcca ttaatcattg aaaatgagca cagaaataga
     1081 aattaattat attcgattaa ttaaaatgtg tattgcttga tgtatttaaa aatattattt
     1141 tcatggtgag agtggaggta aagaagacac aataataaaa aggaatgtga caaataatgg
     1201 ttctggttgg gcgttaaatt taaggtcac
//
LOCUS       DAJTRQ010000279         1197 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000279 DAJTRQ010000000
VERSION     DAJTRQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            4..915
                     /locus_tag="OO332_004207"
     CDS             4..915
                     /locus_tag="OO332_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5944414.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            931..1158
                     /locus_tag="OO332_004208"
     CDS             931..1158
                     /locus_tag="OO332_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944415.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          336 a          253 c          336 g          272 t
ORIGIN      
        1 gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc cggaatgtct
       61 ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga aggtatgcat
      121 gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc aggtacccct
      181 ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac agtgcgctca
      241 ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt tgctgcccgg
      301 gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc acattcgagc
      361 gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc gggaagaaat
      421 aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga aaccagtggc
      481 cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga tggtctggag
      541 caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg caacccgaaa
      601 cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt cagccgtttt
      661 gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt agccaaagca
      721 tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga aaagcccggg
      781 ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa aggtgcaggc
      841 acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag caaccccagg
      901 ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa aatggcaacg
      961 gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata tctcggcgtg
     1021 gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa cgctgtcagg
     1081 attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct taatggctgc
     1141 cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa atggcaa
//
LOCUS       DAJTRQ010000280         1193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000280 DAJTRQ010000000
VERSION     DAJTRQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..848
                     /locus_tag="OO332_004209"
     CDS             <1..848
                     /locus_tag="OO332_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5944416.1"
                     /translation="PKFDETRQYPAIVVSHPGGGVKEQTAGTYAEKLAEKGFVTIAYD
                     ASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMGICAGAGYTANAA
                     IQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNARTSDISSEEYAIMP
                     LAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITYDAYHMAEVYLTQ
                     PMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKAYVAEAISVLAPF
                     FEETL"
     gene            848..>1193
                     /locus_tag="OO332_004210"
     CDS             848..>1193
                     /locus_tag="OO332_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5944417.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVC"
BASE COUNT          318 a          269 c          314 g          292 t
ORIGIN      
        1 ctccaaaatt tgatgaaacc cgtcagtatc cggcaattgt ggtatctcac ccaggtgggg
       61 gcgttaaaga gcaaacggcc ggaacctatg ccgaaaagct ggcggaaaag ggatttgtca
      121 ctattgctta tgacgcatct tatcaaggtg aaagtggcgg cgagccgcgt cagttagaaa
      181 acccatacat tcgtactgaa gacgtcagtg ccgtgattga ttacctgaca acgctttctt
      241 atgtcgacaa tacccgaatt ggtgcgatgg gaatttgtgc cggtgcagga tatacagcca
      301 atgctgcaat tcaggatcgc cgcatcaagg ccatcggtac cgttagtgcg gtcaatattg
      361 gttcgatgtt ccgcaatggt tgggaaaaca atgtgaaatc tattgatgct ctgccacaca
      421 ttgaagctgg ctcaaacgcc cgaaccagcg atattagcag cgaagaatac gccattatgc
      481 cactggctcc aatgaaggag gccgatgcgc cgaacgaaga actgcgtcag gcctgggagt
      541 attaccacac acctcgtgcg cagtatccaa cagcaccggg ttacgctact ttgcgcagcc
      601 ttaaccagat tattacctat gatgcttacc atatggcgga agtgtacctg actcagccga
      661 tgcagattgt ggcgggcagc caggcaggga gcaaatggat gagtgacgac ctgtacgatc
      721 gggcatcaag ccaggataaa cgctatcaca ttgttgaagg cgcaaaccat atggatcttt
      781 atgacggcaa agcctatgtg gctgaagcaa tttcagtatt agctccgttc tttgaggaaa
      841 ctctgtaatg aaacatattc aaatccgcaa tagtgatatg gcctggcata ttgctgccaa
      901 tattcagttt ccaccaaatt ttgatgagtc aaaacagtat ccggcgatta tcagcgtcca
      961 tccttttggc agttgtaagg agcagacgtc tggcaatatc tatggcaagg cattggctga
     1021 aaaaggctat gtcgttctcg cttatgacgc cagtttccag ggagaatctg gtggggagcc
     1081 tcgttggata gaagatccga cacagcgcgt agaggatatt agccgtgtta tcgattatgc
     1141 agtaactctg ccatatgttg atgctgaacg cattggcgtg ctgggtgtgt gtg
//
LOCUS       DAJTRQ010000281         1180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000281 DAJTRQ010000000
VERSION     DAJTRQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..516)
                     /locus_tag="OO332_004211"
     CDS             complement(<1..516)
                     /locus_tag="OO332_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944418.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTC"
     gene            complement(652..>1180)
                     /locus_tag="OO332_004212"
     CDS             complement(652..>1180)
                     /locus_tag="OO332_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HCR5944419.1"
                     /translation="KNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGG
                     MGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAG
                     DGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVLQKSLEIT
                     EEADLALRNAMARIK"
BASE COUNT          275 a          319 c          270 g          316 t
ORIGIN      
        1 gcaggtattg gctttgcaac cgtcgccctg gagccagact gtaccgttaa tggttactgg
       61 cgttacgttt ccgccagggc catccatata cccgccttta ccgaacatgc cgctcagtcc
      121 gaccggtttt aatgctttgt caagcgcagc tttgtaggca gcgtcacctt cgtacaactc
      181 gtacgttgtt ggacacgctc caggttgttc agcccgggcg tttgggtcgc agctgagcgc
      241 ttgtttcacg ctcgctgctt ccacgttaaa gccagtaaac agaaccgcca gaaataacat
      301 cagggtatgt aaacgtttca ttgtaaatat tcttccgttg taaatggcgc tggaaaattt
      361 ctcatcagtg aaaccttccg gaaatttccc ccccttgtat gatgtatatc gccagtattt
      421 tatttcatcc cggatataat ttttatattt cccggcacgt gcaataacgc tgaccagaga
      481 tataaggctc attaataact ttacaggtaa ttgcataatt aaatttccag tgagtgaatt
      541 gacaggtgag gggtaaaact ttctctcagg taatatatat ctgaaaatat tttcaataac
      601 accagctagg aaaaacacca ccacaggcgg tggcgtttct ttggagcgtc tttatttaat
      661 ccgggccatc gcgtttctca gtgccaaatc tgcctcttcc gttatctcca gacttttctg
      721 caacaccgtt tctgaccggg acagtacctg acgtgctgcc agcggtttcc ggatatgctc
      781 catcgtcagc atcgtgtctt catagccctg ctgacgccag gcgtcggtat gtgccaacgt
      841 gaaactgagc agaccgccgc tgttgtcacc atcacctgca tatttcagcc ttttccgggg
      901 acggatctca aggattgtgg tgtccggaaa agcctggcga tcccataaag aaccgtcact
      961 cagatgcgtc acaatcacca tattgcatcc ggcatcgacc agaggcgtca caggggtatt
     1021 tccctgcata tttcgccatc ctcccatacc accatcaccg aacattgtcc cctgaacctc
     1081 acggggacgg aacagcaggg gcagcgcggc tgacgcaagc agagcctctt tctgctgtcc
     1141 gcggggcagg ctctggatat gctgaaaaac ggcgtttttc
//
LOCUS       DAJTRQ010000282         1174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000282 DAJTRQ010000000
VERSION     DAJTRQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..372)
                     /locus_tag="OO332_004213"
     CDS             complement(<1..372)
                     /locus_tag="OO332_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944420.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQ"
     gene            complement(374..652)
                     /locus_tag="OO332_004214"
     CDS             complement(374..652)
                     /locus_tag="OO332_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944421.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(719..969)
                     /locus_tag="OO332_004215"
                     /pseudo
     CDS             complement(719..969)
                     /locus_tag="OO332_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          295 a          304 c          293 g          282 t
ORIGIN      
        1 ctggttgtcg cagtgccagc acaacaccat tgcgccggta ccataacggt gaatgacggt
       61 ttcgctgtga tgataatcgc cgtgtggcca ctggcaggat ttaatatggc gcaacagcca
      121 gtcagacaat gcaccagcac caccagcagc acgaatcacc cgtgcgttac tgaaaaacgg
      181 cagcaatgtt ttgtcttcca ccagcggctg gcgagcggca ggaacgaccc cggacggcag
      241 attacgcatg cttttcggtt ccggctccac cagtaaccgg gtattgtgga ataccggcat
      301 ggattcacga ccgggcttaa ggaccaccag cccgagttcc ggaaccagaa caggtcgaag
      361 taatacccgc acgttacctc cagatccgtt gctggaatgt gcgggacgga cgcggtggct
      421 gttcggagta agggagcctg acggagatta tccagtgacg gtagtcgagg ctaagggctt
      481 ttttaacctc gcatccgcgc ctgcggtaac actgaatgag ccattcggcc tgttcttcag
      541 tgcatgaggg atgctggtac cagtctgact taaatgcgtg agaataccgc tcgtgcgtgt
      601 gggcaagaac ggtcgaatta tcatgattgt aatattttgc gttgcgtgcc atcggttttc
      661 tccggtggca cggtgttact cagcgggagt tcagccccgc gcaagattgt agatgagttt
      721 attcttctga aaaagcagaa aagccagctt ttattccgat ctctttcaat gcctgtaatg
      781 aagtgacaaa ctcaccgtcg cgcaagataa atccgtccgt cactcgggca tccacaaaat
      841 taattaacgc agccccattt tttgcaaaca cacaatgcgg taatgactaa caatatttcc
      901 attttcaacg cacacagcat agaggccatc ttcacaaaaa attttacgca gttcttcgat
      961 gttcatcatc agaatccttc cagataagta gcactcccct gttcggggtc catccctttt
     1021 atccctgcgc gctacttaag tatttttgat tctattccgg caccatccag aacttcaaat
     1081 gcgttgaaaa taaaaacaaa aacccgccga agcgggttaa gtgcgggtgc gttgaggatg
     1141 cctgccacat cagaggtggc gagggatttc tcac
//
LOCUS       DAJTRQ010000283         1143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000283 DAJTRQ010000000
VERSION     DAJTRQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>1143
                     /locus_tag="OO332_004216"
     rRNA            <1..>1143
                     /locus_tag="OO332_004216"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          286 a          262 c          359 g          236 t
ORIGIN      
        1 agccatgccg cgtgtatgaa gaaggccttc gggttgtaaa gtactttcag cggggaggaa
       61 gggagtaaag ttaatacctt tactcattga cgttacccgc agaagaagca ccggctaact
      121 ccgtgccagc agccgcggta atacggaggg tgcaagcgtt aatcggaatt actgggcgta
      181 aagcgcacgc aggcggtttg ttaagtcaga tgtgaaatcc ccgggctcaa cctgggaact
      241 gcatctgata ctggcaagct tgagtctcgt agaggggggt agaattccag gtgtagcggt
      301 gaaatgcgta gagatctgga ggaataccgg tggcgaaggc ggccccctgg acgaagactg
      361 acgctcaggt gcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg
      421 taaacgatgt cgacttggag gttgtgccct tgaggcgtgg cttccggagc taacgcgtta
      481 agtcgaccgc ctggggagta cggccgcaag gttaaaactc aaatgaattg acgggggccc
      541 gcacaagcgg tggagcatgt ggtttaattc gatgcaacgc gaagaacctt acctggtctt
      601 gacatccacg gaagttttca gagatgagaa tgtgccttcg ggaaccgtga gacaggtgct
      661 gcatggctgt cgtcagctcg tgttgtgaaa tgttgggtta agtcccgcaa cgagcgcaac
      721 ccttatcctt tgttgccagc ggtccggccg ggaactcaaa ggagactgcc agtgataaac
      781 tggaggaagg tggggatgac gtcaagtcat catggccctt acgaccaggg ctacacacgt
      841 gctacaatgg cgcatacaaa gagaagcgac ctcgcgagag caagcggacc tcataaagtg
      901 cgtcgtagtc cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc
      961 gtggatcaga atgccacggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc
     1021 atgggagtgg gttgcaaaag aagtaggtag cttaaccttc gggagggcgc ttaccacttt
     1081 gtgattcatg actggggtga agtcgtaaca aggtaaccgt aggggaacct gcggttggat
     1141 cac
//
LOCUS       DAJTRQ010000284         1117 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000284 DAJTRQ010000000
VERSION     DAJTRQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..764
                     /locus_tag="OO332_004217"
                     /pseudo
     CDS             1..764
                     /locus_tag="OO332_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(773..>1117)
                     /locus_tag="OO332_004218"
     CDS             complement(773..>1117)
                     /locus_tag="OO332_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944422.1"
                     /translation="LPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPESNG
                     IAESFVKTIKRDYISAMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRA
                     SNGLSDNRCLEI"
BASE COUNT          280 a          266 c          264 g          307 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cagtgcacta tgcggatgcc attcgttata atgctcgaac gcctctgcaa
      901 ggttctttgc tgccgttaac ccgtctggtt tgggcatggc actgatgtag tcacgcttta
      961 tcgttttcac gaagctctct gctatgccgt tactctccgg actccgcacc gccgtgctct
     1021 tcggttcaag ccccaacatc cgggcaaact gccgtgtttc attagcccgg tagcatgaac
     1081 cattatccgt cagccactct actggagacg ccggaag
//
LOCUS       DAJTRQ010000285         1109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000285 DAJTRQ010000000
VERSION     DAJTRQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            318..839
                     /locus_tag="OO332_004219"
     CDS             318..839
                     /locus_tag="OO332_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5944423.1"
                     /translation="MDVYANGQHVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQ
                     KLINGTWYNVASI"
     gene            842..>1109
                     /locus_tag="OO332_004220"
     CDS             842..>1109
                     /locus_tag="OO332_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944424.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRIT"
BASE COUNT          321 a          227 c          285 g          276 t
ORIGIN      
        1 aaaacgactc aatccttgcc tggattcgga atactgactg ggcgaagatt ggatttaaaa
       61 atgattcgga tgcagacacc gattcctaca tgtggtttga aacaggtgac aacggcaatg
      121 agtatttcaa atggagacac cgcctcgctg gcggccagct caaagagctg atgaatctta
      181 aatgggattc actaaatatt ctggttaatg ccgtcattaa tggttgcctt ggcattggta
      241 cgacgaatgc gttaggtgga aattcaattg ctttcgggga taacgatacc ggcctgaaac
      301 agaacggcga tggtctgctg gatgtttatg cgaatggaca gcatgtattt cgttttcaga
      361 atggcgtggc tattgctttt aagaatattc aggccggaac tgccagaaaa ttcacgttat
      421 ccagcgccaa caactccacg aaaaatgcag cgttttattt gtggggtaat ccatccaggc
      481 ctgttgttgc agagcttggc gatgattcag gctggcattt tttcagccag agaaacccgg
      541 ataacagcat agtgttcact gttaacggac aggtaattcc gttaaattac ggaaacttcg
      601 atgcccgcta taaatatcga acagaggggg tacaggatgt acggtatggc catgaaatgt
      661 attacagccc cggcagtaac accgtttcgt ggagattttg cgcaccttcg ggacacgggc
      721 tgtcagggat ggcgatatcg gataccggcc gtaactcagc ggataacgtt gacggtgtgt
      781 attaccgacc actgcaaaaa ctgattaatg gcacctggta taacgtagcg agtatttaac
      841 aatgttgcat ttaaaaaata ttactgcggg taatccgaaa acagcagaac aatatcagat
      901 gacaaaacga tattcggtca cctggctttt ttcagaagac ggaaaaaact ggtatgaaga
      961 gctgaagaat ttcgccagcg acacaataaa aatagcttac accggagatg gtcgcgtggt
     1021 gtgggtcggt aaggatgtga caggcatcga gccacgcaat gccagcgtta tcgaagttcc
     1081 ggatattacc gctaaccgac ggattaccg
//
LOCUS       DAJTRQ010000286         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000286 DAJTRQ010000000
VERSION     DAJTRQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..123)
                     /locus_tag="OO332_004221"
     CDS             complement(<1..123)
                     /locus_tag="OO332_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944425.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHL"
     gene            complement(195..1079)
                     /locus_tag="OO332_004222"
     CDS             complement(195..1079)
                     /locus_tag="OO332_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR5944426.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          293 a          317 c          229 g          261 t
ORIGIN      
        1 tagatgtcct ttcagccaaa tagcgtataa cgaccaccac agcgaccagg aaataaaaaa
       61 gagaaatgag taactggatg caaaacgata gtacgcattt ctgaatggaa tattcagtgc
      121 cataattacc tgcctgtcgt taaaaaattc acgtcctatt tagagataag agcgacttcg
      181 ccgtttactt ctcactattc cagttcttgt cgacatggca gcgctgtcat tgcccctttc
      241 gccgttactg caagcgctcc gcaacgttga gcgagatcga taattcgtcg catttctctc
      301 tcatctgtag ataatcccgt agaggacaga cctgtgagta acccggcaac gaacgcatct
      361 cccgcccccg tgctatcgac acaattcaca gacattccag caaaatggtg aacttgtcct
      421 cgataacaga ccaccacccc ttctgcacct ttagtcacca acagcatggc gatctcatac
      481 tcttttgcca gggcgcatat atcctgatcg ttctgtgttt ttccactgat aagtcgccat
      541 tcttcttccg agatcttgac gacatccgcc agttgtagcg cctgccgcaa acacaagcgg
      601 agcaaatgct cgtcttgcca tagatcttca cgaatattag gatcgaagct gacaaaacct
      661 ccggcatgcc ggatcgccgt catcgcagta aatgcgctgg tacgcgaagg ctcggcagac
      721 aacgcaattg aacagagatg taaccattcg ccatgtcgcc agcagggcaa gtctgtcgtc
      781 tctaaaaaaa gatcggcact ggggcggacc ataaacgtaa atgaacgttc cccttgatcg
      841 ttcagatcga caagcaccgt ggatgtccgg tgccattcat cttgcttcag atacgtgata
      901 tcgactccct cagttagcag cgttctttgc attaacgccc caaaaggatc atcaccgacc
      961 cgacctataa acccacttat tccgcctaat ctggcgattc ccaccgcaac gttagctggc
     1021 gcgccgccag gacaaggcag taggcgcccg tctgattctg gcaagagatc tacgaccaca
     1081 tcccctaaaa cccatacttt
//
LOCUS       DAJTRQ010000287         1098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000287 DAJTRQ010000000
VERSION     DAJTRQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>1098
                     /locus_tag="OO332_004223"
     CDS             <1..>1098
                     /locus_tag="OO332_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5944427.1"
                     /translation="EMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQA
                     RQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQT
                     HAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKG
                     YYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLG
                     NEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSNRWEIELGYREIKQTMQLS
                     RLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRML
                     MTLQGASPGRIPELMRDLASMGQLVKLPTRR"
BASE COUNT          291 a          271 c          324 g          212 t
ORIGIN      
        1 tcgaaatgat ggtctggtgt attgttggca tggcgcttga gcgtaaagaa cctcttcacc
       61 agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt gcccccagtg
      121 ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg ttcacgaaaa
      181 cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg accctgctgg
      241 ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca gccttccccc
      301 gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc tgccagatgg
      361 aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac agcgaaaatg
      421 agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta atggataaag
      481 gttattactc actgggactg ttaaatggct ggagcctggc gggagaacac cgccactgga
      541 tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt aaaggcgatc
      601 atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga ctgggaaatg
      661 aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat ctgctgacgt
      721 cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac agtaatcgct
      781 gggaaatcga actgggatac agggagataa aacagacgat gcaactgagc aggctgacgc
      841 tgagaagtaa aaagccggag cttgtggagc aagagctgtg gggtgtctta ctggcttata
      901 atctggtgag atatcagatg attaaaatgg cggaacatct gaaaggttac tggccgaatc
      961 aactgagttt ctcagaatca tgcggaatgg tgatgagaat gctgatgaca ttgcagggcg
     1021 cttcaccggg acgtataccg gagctgatgc gcgatcttgc aagtatggga caacttgtga
     1081 aattaccgac gagaaggg
//
LOCUS       DAJTRQ010000288         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000288 DAJTRQ010000000
VERSION     DAJTRQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..336)
                     /locus_tag="OO332_004224"
                     /pseudo
     CDS             complement(1..336)
                     /locus_tag="OO332_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          365 a          207 c          186 g          338 t
ORIGIN      
        1 tatacggtat cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc
       61 acaatactga atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct
      121 gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc
      181 ctttttacca gtgctgattt ccacattccg tccaacagag cttatagcct ttccctggat
      241 tatagcattg tccggctgaa gttctttttg aataataata gaagcactgc tggcagatcc
      301 agtccgtttt tcataaccca ctgtactgat aaccataatc taatcagtag aaattgagtc
      361 gaaaataagc actactccat acaggataat tagaggtcag tttgattatt cacaattcat
      421 catcagcatt ttctatttct gacgaaatca acatgaaaat aaccatatat gataattatt
      481 ataataacgg ctttaattgg aatacatata ttacaacgta ttatatataa ttggtattct
      541 gggaactata ttctcaaaat acagtagaaa cgaggtatgt ttctggtgga aaggacagtg
      601 ggattaaaaa gtaagaattg ataaaaaaaa cgccagcaac acatagtgcc ggcggaggga
      661 ataccccatg gagaaaatgt gatgcctaga gcatcatgaa tatataatta aaaatagtta
      721 gcgttgtcaa tactttcaca aaaataattt tcgtagtata gaaagatatt tttatgcatg
      781 acctacctga atttgctccg ggtagaggtt ataaataaaa attgaatcac gacaaacaca
      841 atattcatag tatggcgatg cctacgccag caagaatagc gccaataata ttgggaatat
      901 aatagacacc agacgcacag gcatctcact ccttaacaaa caataatcag gatatttact
      961 tttacccagc taactgttta cacccaaagt gcacacaatt aaccattcaa taacaaatgg
     1021 caatatccat agccatacga ctttaccttg taatgttcgg tatttcttta taattattct
     1081 gggaaatcta tcattt
//
LOCUS       DAJTRQ010000289         1068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000289 DAJTRQ010000000
VERSION     DAJTRQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..732)
                     /gene="ospC2"
                     /locus_tag="OO332_004225"
     CDS             complement(<1..732)
                     /gene="ospC2"
                     /locus_tag="OO332_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5944428.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHE"
BASE COUNT          350 a          156 c          186 g          376 t
ORIGIN      
        1 ctcatggtaa aaaacaggtg gaatagcatt atcaaagtgg tcggttaatg tcatatatcc
       61 atatggagag tcatgatcaa tgatatacgc attagcacca aaatccacag tatgatgtgt
      121 tttatttagt gggagtgttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc
      181 gtggttagct atttcttcaa tatcctcaga gaatgtgttt ttatatgttt gtatattctc
      241 agacttaatt ttatcaaagg acttaatatt aagagtatta tttgacaggt tagcgttact
      301 ttgatgtctg aagttaaaat cagaattaat aataatagag agcaagtgac gttcatccga
      361 agaaagagga tgagtttttt gtattatttc agaaagatag tgtctggaca ccttatcgag
      421 gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttaa
      481 tgatttgaaa ctggtccctt gagagaacat tttactataa ctttgcgctg ctatagactt
      541 ccttaaaaag cttttcacag tatcagcaca gtgatctaaa tgtttttcac ttatgccaga
      601 agaggatgag tttgttacgc atgatatgtt tttttgtaat gcttttgtat ctttgttcgg
      661 atttattttt ccatcaacaa gatcaatatt gttctgaaca ttaatatgat ttactgcttc
      721 aggtattttc ataaaacatc ccccattatg tgcgttttta tctgcagtta tttagaataa
      781 tattatctaa cttaatattt tcttgtatgt attataacga acatatttta atatgttgta
      841 cgattttcac tctgaaatcc cctgagttta atcacacttc ctttactgaa aggaattatt
      901 gcaacagtat tatttgtttt tggatatata tcttgcgcct tgttatgtag ttaatgctca
      961 gaatatgata gataaaatag agacgcaaaa cagggcatgc tcataacatg atgataacat
     1021 aaaaaattag taacaataaa tgaaagaatc atgggcgctc aacaaaaa
//
LOCUS       DAJTRQ010000290         1066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000290 DAJTRQ010000000
VERSION     DAJTRQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.296"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..1029
                     /locus_tag="OO332_004226"
     CDS             <1..1029
                     /locus_tag="OO332_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5944429.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT          327 a          235 c          286 g          218 t
ORIGIN      
        1 gcgtggagag taaaaaatct cggggaatca ccgaaaaagc tattcaagga agcctgcata
       61 cggtggctgc gtgagaaatc ggataagaag tccattgatg atgacaagag cattatatcg
      121 ttctggatgt tgcacttcag agaagccatt ctctctgaca taacaacaga aaaaataatg
      181 gaggcggtag acgggatgga aaaccgccgc catcgcctga actgggagat gagccgggac
      241 aggtgtttgc gacttggcaa tccggtgccg gagtataaac caaagcttgc aagcaaagga
      301 acgaagacga ggcatctggc aatacttcgc gccattctca atatggctgt tgaatgggga
      361 tggcttgaca gggcacccaa aatatcaaca ccacgcgtta agaatggacg catcagatgg
      421 cttacagagg aggaatcgaa gcgcctgttt gcagaaattg ctcctcactt cttccctgtg
      481 gtcatgtttg caatcacgac aggccttcgc cgttccaacg ttacagacct tgagtggtca
      541 caggtcgatc tggataagaa aatggcatgg atgcaccctg atgaaacaaa agctggcaat
      601 gcgatcggag ttcctcttaa cgaaaccgca tgccagatat taagaaaaca gcaggggctc
      661 cataagagat gggtatttgt ccacaccaaa cctgcctacc gaagcgacgg aacaaaaaca
      721 gcagcggtaa ggaagatgag aaccgacagc aacaaggcat ggaagggagc gttaaagcgg
      781 gcaggcatta gcaacttccg cttccatgat ctgaggcata cctgggcaag ctggctggtt
      841 cagtccggtg tctctcttct tgcacttaaa gagatgggag gatgggaaac tctcgaaatg
      901 gttcaaagat acgcccacct ttcagccggg catctcaccg agcacgcaag caaaatcgat
      961 gcgattataa gtcgcaatgg cacaaatacg gcacaagagg agaacgtggt ttacttaaat
     1021 gcgaggtaac ttattgattt aaatggtgcc gataatagga gtcgaa
//
LOCUS       DAJTRQ010000291         1054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000291 DAJTRQ010000000
VERSION     DAJTRQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..213
                     /locus_tag="OO332_004227"
     CDS             <1..213
                     /locus_tag="OO332_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5944430.1"
                     /translation="IQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVL
                     WNWILFRKFSSPEEVENVAAQLLEFA"
     gene            213..503
                     /locus_tag="OO332_004228"
     CDS             213..503
                     /locus_tag="OO332_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5944431.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            500..1036
                     /locus_tag="OO332_004229"
     CDS             500..1036
                     /locus_tag="OO332_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5944432.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASQKMTKTAVNEALRRLKKAGIDKPELEAFLREMIEGKQKSWLTHCTDAEAL
                     KIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWALITCRRRVDQWL
                     RVAEFILYSPMREAFDYA"
BASE COUNT          303 a          211 c          297 g          243 t
ORIGIN      
        1 attcaggccg gattttacac cgagcatatt tatccggacg gtagccgtgg gcgcagggca
       61 aaatctatcg catttgcgaa catggacgaa accgagtttc agcaggttta taaatctgta
      121 ctgaatgtgc tgtggaactg gatcctgttc cgtaaatttt cctctccgga ggaagtcgaa
      181 aatgtggccg cgcagttact ggagtttgcg taatggtgga tttacgtaaa gcggcgcggg
      241 ggcagatgtg caccgtcaga attcctggct actgcaatca caatcccgaa acttctgtgc
      301 tggcgcatta caggctggcg gggacgtgcg gaacagcgac aaaaccacac gatatgcagg
      361 cagcgattgc ctgcagctca tgccacgatt taatcgacgg gcgggtaaaa accagcgatt
      421 acaccaaaga agaattacgc ctgatgcatg cagaaggtgt ttttcgcaca caagaaatct
      481 ggagaaagga gggatatttg tgatttaccc aacaaataca ggcaaaagcg gggaacacct
      541 tcgtctcacc acgctggaaa gtgtctggat tcagggaaaa ctgcgcatgt gggggcgctg
      601 gtcgtatatt ggcggcggca ggtcagggaa tatgtttaac cagttgctgg catcccaaaa
      661 aatgacaaaa acagccgtca atgaagccct gcgcagattg aaaaaagcgg gaattgataa
      721 accagagttg gaagcatttt tgcgtgagat gatcgaagga aagcaaaaaa gctggttaac
      781 tcactgcact gacgcagagg cactaaaaat tgatagtgtt ataggtgaag ttctggcgga
      841 tcatccagga ctactaaatg tcctgagtca gcgttatgtg gggcgaggga tgagtaagag
      901 aaggatggcc gagttattaa acgaacagta cccagagtgg gcgttgatta catgccgacg
      961 tcgtgttgat cagtggttgc gtgttgctga gttcattttg tattcaccga tgagagaagc
     1021 gttcgattat gcttaaaaaa tcattgcaaa atga
//
LOCUS       DAJTRQ010000292         1045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000292 DAJTRQ010000000
VERSION     DAJTRQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>1045
                     /locus_tag="OO332_004230"
     CDS             <1..>1045
                     /locus_tag="OO332_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5944433.1"
                     /translation="TPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILG
                     VKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLP
                     DTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVS
                     VTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRR
                     SLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDK
                     PEPVAKVCYAQMVK"
BASE COUNT          240 a          270 c          334 g          201 t
ORIGIN      
        1 tactccccgc ccgctgaaac gcctgttcac agccaaccag tgctggacgt ccttcctgga
       61 tgcgggcggt ctgcgcgata tcgaggttga ggctgtcacc aaaatgctgg cctgcggcac
      121 gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc acgtcagata
      181 cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct
      241 gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc tggtcttcac
      301 cctgccggac acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg
      361 ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat
      421 cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc
      481 tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga
      541 cgcgatgcgt tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag cgtggtcaga
      601 ggggctggcg atgccggagt cgttgtcaca tatcacgacg gaatcacaga ggagaagcct
      661 ggtgctgaaa gccggaggaa aatactggca tgtgtacatg tcgaaaaaaa cggccggcgg
      721 gaggaatacg gcgcgctacc tgggtcgtta tctgaagaag ccgccgatag cggcctcccg
      781 gctggcacat tacaacggag gggcgagcct gagcttccgt tacctggacc acaaaacggg
      841 agaaacggcg acggaaacgc tgacacagcg tgagctggtc gcgaggctga aacagcacat
      901 cccggagaag ttttttaaga tggtgaggta cttcgggttc cttgccagcc gggtgtgtgg
      961 agagaagctg ccgcaggtgt accgtgctct ggggatggat aaaccggaac cagtggcgaa
     1021 agtgtgctat gcacaaatgg tgaaa
//
LOCUS       DAJTRQ010000293          974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000293 DAJTRQ010000000
VERSION     DAJTRQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>974
                     /locus_tag="OO332_004231"
     CDS             <1..>974
                     /locus_tag="OO332_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5944434.1"
                     /translation="SRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSLRTK
                     DRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPESYQL
                     YVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLEPET
                     GSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTLIEI
                     FDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDVVTI
                     NNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPE"
BASE COUNT          253 a          262 c          229 g          230 t
ORIGIN      
        1 tcaagaacct acctttatca gcgtaatggt gtcttttaca tccgtcttag gatgaagacc
       61 actggtcgcc ttaccgcttc gcttccttct cacaaccgtt ataaactagc gtcagtatct
      121 ttacggacca aggatagacg taccgctatg gctcactccc ggcacatcaa gtcagcactt
      181 aaagcaattc atgcagacaa ccctaacgcc tcttatgagg agctacggga gcacctgaag
      241 accattgtcg agtgggaact cagtgtcagc cgcgatgacc tgaacgaccc tgagtcctat
      301 cagctttacg ttgaccagta cgacgatatt aagtccaacc ttcgggaagc tgtagcgact
      361 gagcacctga ctgtagacca gcatcgctat atcaatgacg ttatcggtgt gcttaaagcc
      421 tgtcaggata gactcaaagg tgatagctct ggcctattgt cttaccttga gcctgagact
      481 ggcagtctaa gaccgtccgt ctccctatct gtattggcgg aacctgaagt ccctgaacct
      541 aaagcactga ccctcgcgtc tctcattgag cagtacgagc aggagaacgc ccagaactgg
      601 aagccagcga ccctaagcga aaaccgagcg tcacactcta cgctaatcga gatattcgac
      661 tatctggata ttcaggatgt cggcaaggcg acccgtgcag atatgctcag agttcgtgaa
      721 gtcctccagc agctaccgaa gaaccgcaag caacgcttta agtctatgcc gctgtctgac
      781 ttgctgaacc gggagtctaa gactgactgt ctggacgtcg ttaccattaa caataaatat
      841 cttatcaaga tggctgctgt gtttaagtgg gcggtacgca acgacttgat agctaagaac
      901 ctgaccgaag gtcttgagct gaaagtccct cagcgtaaag cctccgacgc tcgtgatgcg
      961 ttcagtcctg agca
//
LOCUS       DAJTRQ010000294          971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000294 DAJTRQ010000000
VERSION     DAJTRQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(684..>971)
                     /locus_tag="OO332_004232"
     CDS             complement(684..>971)
                     /locus_tag="OO332_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944435.1"
                     /translation="EAEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYE
                     IQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          264 a          245 c          171 g          291 t
ORIGIN      
        1 agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg
       61 taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct
      121 tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg
      181 agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg
      241 tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca
      301 gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa
      361 cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca
      421 tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc
      481 tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct
      541 aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa
      601 aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa
      661 cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca
      721 ttaatctggt tgccggatgc tggtggattt tcgcgtttta gttgttcata aaagtgcaca
      781 gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt
      841 tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca gtcaccaacc
      901 cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg ccacgctatc
      961 ggctctgctt c
//
LOCUS       DAJTRQ010000295          964 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000295 DAJTRQ010000000
VERSION     DAJTRQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(37..256)
                     /locus_tag="OO332_004233"
                     /pseudo
     CDS             complement(37..256)
                     /locus_tag="OO332_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(388..>964)
                     /locus_tag="OO332_004234"
     CDS             complement(388..>964)
                     /locus_tag="OO332_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5944436.1"
                     /translation="MNQVARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNFRD
                     SFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLH
                     IDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNIT
                     FQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          313 a          209 c          187 g          255 t
ORIGIN      
        1 cattcagccg catactgaca gtgagaaact gaaagattat tgctgatccc tgtggctgac
       61 gagtctttct cttaaatcga ttaaccagat tgatcgccag ccaaacaagg gcattaagca
      121 aatttatcgt cacaagcact tctcccagcc cggtactggg ttttgcaata aggatgacca
      181 ggagggtgat cagcataaat gcaatggaaa agatgatata gagagtgaaa taatccagat
      241 tattactatt gttcatgcta ctaatggcaa tcctgtcaaa actttcaaac aattatagca
      301 aacaaaataa tgaacaatag taacaagagg caacgagcta attagctgtc tataattaca
      361 ccgcactatg gccttactga tcaattttca ctcaaccaca accttcacct tcggtttaga
      421 aaggataatt ttgaccaggt cgatgtatgt aacaggttta tagaaataat aaccctgctg
      481 aaatgtgata ttattttggt tcagatagcc aagttgttct ttcgtttcga caccttcagc
      541 gacgatactg atcgaaagtt tacgcgccag atccaataca caatccagaa ttcgggttga
      601 ttctgggtct gcgtttacgc ggccaacgaa actgtgatca attttgatat aatcaatatg
      661 caggtcatga agataagaaa gccctgagta gccagtaccg aaatcatcca gcgcgatgac
      721 aaaaccattt tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg
      781 ctcacgctcg gtgacttcca gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa
      841 atttaaacac tcgtcgacaa acgtcggcga aataatatgc gaagcgctaa aattaattcc
      901 aatatgaaaa ccttccggca gtttactcgc gatagcgttc atctgtctgg caacctgatt
      961 catc
//
LOCUS       DAJTRQ010000296          953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000296 DAJTRQ010000000
VERSION     DAJTRQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            105..>953
                     /locus_tag="OO332_004235"
     CDS             105..>953
                     /locus_tag="OO332_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5944437.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          322 a          193 c          164 g          274 t
ORIGIN      
        1 gaagcaatac ctggagaaag agtacaaagc aaactgtatt aaacttaata agttatattg
       61 tatttcacta gagttcttat cgttgataag aaattctggt tataatgatt aaatcaacaa
      121 atatacaggt aatcggttct ggtattatgc atcaaataaa caacatacac tcgttaactc
      181 tattttcttt gcctgtgagt ttgagtccat cttgtaatga atattattta aaagtctgga
      241 gtgaatggga aaagaacggt accccaggcg agcaacgcaa tatcgccttc aataggctaa
      301 aaatatgttt gcaaaatcaa ggggcggaat taaatctatc tgagttagat ttaaaaacat
      361 tacctgattt accgcctcag ataacaacac tggaaataag aaaaaaccta ttaacatatc
      421 tccctgattt cccaccaatg cttaaggtaa tacatgctca atttaatcaa ctggaaagct
      481 tacctgcctt gcccgagacg ttagaagagc ttaatgtggg tgataacaag ataaaagaat
      541 taccatatct tcctgaaact ctaactcatt tacggatcca taataaccga ttgcatattc
      601 tgccactatt gccaccggag ctaaaattac tgatggtttc tggaaacaga ttagacagca
      661 tccccccctt tccagataag cttgaagggc tggctctggc taataatttt atagaacaac
      721 taccggaatt accttttagt atgaacaggg ctgtgctaat gaataataat ctgacaacac
      781 ttccggaaag tgtcctgaga ttagctcaga atgccttcgt aaatgttgca ggtaatccat
      841 tgtctggcca taccatgcgt acactacaac aaataaccac cggaccagat tattctggtc
      901 ctcaaatatt tttctctatg ggaaattctg ccacaatttc cgctccagaa cac
//
LOCUS       DAJTRQ010000297          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000297 DAJTRQ010000000
VERSION     DAJTRQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(105..428)
                     /locus_tag="OO332_004236"
     CDS             complement(105..428)
                     /locus_tag="OO332_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5944438.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            712..906
                     /locus_tag="OO332_004237"
     CDS             712..906
                     /locus_tag="OO332_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5944439.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          275 a          244 c          228 g          205 t
ORIGIN      
        1 cgttttcagc tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta
       61 ccgtgttcat accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc
      121 ctggataaca gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg
      181 tccccgatac gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact
      241 tctttcagta cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt
      301 aaaaaaccgg aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct
      361 tctgccgtca gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt
      421 tttgtcataa aacatggctc cgtatctgac atgggtgtcg gggcaaagcc ctgaccaggg
      481 caattgtaat agcgtgcatg tatgcgcggt ataacaattg cacatcctgt cctgtcagca
      541 agctcgaatt accgacaaag gcacggcagt tttttggcag ttacagtgtt gtctgacaga
      601 aatgcccgtc aaaaagccag gcggcagaat agcggcagca aaacagtcga aaagtggtac
      661 gcgatataat cacataacgc agatgacaga cagaacggag aaaacgaaaa gatgaacaag
      721 cagcagcaag ccgtactcaa catggcggga ttcataaaaa gccagagcct gacgctgctc
      781 gaaaaactgg acgcactcga tgctgacgag caggccacca tgtgtgagaa actacacgaa
      841 ctcgcagaag aactccagaa cagcatacag acacgctttg aagtagaaaa tagcacagag
      901 agataacgct caggacagga tgtcgaacgg cacaacagag cgtaaggaga aa
//
LOCUS       DAJTRQ010000298          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000298 DAJTRQ010000000
VERSION     DAJTRQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..483
                     /locus_tag="OO332_004238"
     CDS             <1..483
                     /locus_tag="OO332_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944440.1"
                     /translation="EQYELTKNFDVIWLWTEDGKNWYGEVKNFQPDTIKIVYDENNII
                     VAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVVKRIYTADEQQQQAES
                     QKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLVSRVDTAKPEWPQKPE
                     "
     gene            complement(512..>952)
                     /locus_tag="OO332_004239"
     CDS             complement(512..>952)
                     /locus_tag="OO332_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944441.1"
                     /translation="EDGKNWYEEQKNFQPDTLKMVYDHNGVIICIEKDVSAINPEGAS
                     VVEVPDITANRRADISGKWMFKDGIVIKRTYTGEEQRQQVENEKQSLLQLVRDKTQLW
                     DSQLRLGIISAGNKQKLTEWMLYAQKVESTDTSSLPVTFPEQPE"
BASE COUNT          254 a          219 c          223 g          256 t
ORIGIN      
        1 gaacagtacg aattaacaaa gaattttgac gtaatctggt tgtggacaga agacggaaaa
       61 aactggtatg gggaagtgaa gaactttcag ccagatacaa taaagattgt ttacgatgaa
      121 aataatatta ttgtcgccat cactaaagat gcctccacgc ttaatcctga aggctttagc
      181 gtcgttgagg tccccgatat aacagcaaat cgtcgtgccg atgattccgg gaagtggatg
      241 tttaaggacg gagctgtggt taagcggatt tatacggcag acgaacagca gcaacaggcc
      301 gaatcacaaa aagccgcatt gctttccgaa gctgaatcag tcatccagcc gctggagcgc
      361 tctgtcaggc tgaatatggc aacagatgag gaatgcgcac gactggagtc atgggaacgc
      421 tacagtgttc tggtcagccg tgtggatacg gcaaagccag aatggccaca aaaaccagag
      481 taaaaattaa ggcccgatag cgggccttct ctcattctgg ttgttcggga aacgttactg
      541 gcagactgga ggtgtctgtg gattcgacct tctgcgcgta gagcatccac tcggttaatt
      601 tctgcttatt cccggcggaa atgatgccca gtcgtagctg tgagtcccat agctgggttt
      661 tatccctgac aagttgcaac aggctttgct tttcattttc cacttgttgc ctctgctctt
      721 ccccggtata agttcgcttt atcactatgc catctttgaa catccattta cctgaaatat
      781 cagcccggcg atttgctgta atatcaggaa cctcaacgac gcttgcgcct tccggattaa
      841 ttgctgaaac atccttttca atacaaataa taacgccgtt gtggtcatag accattttca
      901 acgtatcagg ctgaaagttc ttttgttcct cataccagtt tttcccatcc tc
//
LOCUS       DAJTRQ010000299          929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000299 DAJTRQ010000000
VERSION     DAJTRQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            373..>929
                     /locus_tag="OO332_004240"
     CDS             373..>929
                     /locus_tag="OO332_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5944442.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          329 a          180 c          154 g          266 t
ORIGIN      
        1 gtttaagctc cattttcaaa aaaatatatc agggtttgtt actaataact tctgaaaaca
       61 gaaaaaaata gagcggtaat tctatttcgg agaggagtaa ctccatctat cctcgatacg
      121 tactgaacgt tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga
      181 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      241 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      301 aactgtttta aaattaataa gccatattct atttaaccag aattcttatc gttgataaga
      361 attctggtta taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat
      421 caaataaaca atatatactc gttaactcca ttatctttac ctatggaact gactccatct
      481 tgtaatgaat tttatttaaa agcctggagc gaatgggaaa agaacggtac cccaggcgag
      541 caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta
      601 aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat aacaacgctg
      661 gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata
      721 catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt
      781 aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta
      841 cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg
      901 gtagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTRQ010000300          891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000300 DAJTRQ010000000
VERSION     DAJTRQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            359..>891
                     /locus_tag="OO332_004241"
     CDS             359..>891
                     /locus_tag="OO332_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5944443.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALY"
BASE COUNT          355 a          191 c          149 g          196 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tatctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagta gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaatactta caccgacacg
      301 cccttaaaca aaagaaaaaa aaacaataaa aaacaccgca aaaatggatt gttaacaaat
      361 gaaaatactc aattcgaatc tcataacaaa catatcatca acaataagaa atcaccttct
      421 aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa caagtcatcg
      481 tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa cacaacttgt
      541 cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat tcacgcgtca
      601 aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt atacaaaaga
      661 caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc atactgaagc
      721 tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag ttctagcgaa
      781 cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaag ccatatatag
      841 aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt a
//
LOCUS       DAJTRQ010000301          881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000301 DAJTRQ010000000
VERSION     DAJTRQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..303
                     /locus_tag="OO332_004242"
                     /pseudo
     CDS             1..303
                     /locus_tag="OO332_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            472..>881
                     /locus_tag="OO332_004243"
     CDS             472..>881
                     /locus_tag="OO332_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944444.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPG"
BASE COUNT          262 a          155 c          173 g          291 t
ORIGIN      
        1 gagagacgta cagtggtttt gtccgctcac accagtacat acaggtacgg aaataaaacc
       61 agtcgaaatg ccggtaacga cgattactcc tgtctctgat gccggtggac tacaagattt
      121 tatttactgg cgtcctgatg cggacaggac tggtatggaa cctgtttatg tgatacttaa
      181 tgatcctctg gattccgggc gatttacgcg taaacaacta aataaaaata tcttaaacat
      241 gcaagtaatt ttggtattct cgatacaaga ataaatagta gtaactacta tagttgcaaa
      301 taatgttaaa aacatttcta tgatcatctt tacatttttt atgattgaca attgtttttg
      361 gttaaaaaaa gacaactaat gagtgtgata atgcaatact ttgagtaatt atataatgat
      421 aagtaaacag gtttgcgaca attggttatc agtattacag aaggattaaa tatgtcatta
      481 ttcacagaac ctaaggaaat tgaaagatta ccctctgaag agattgaacg attatatccc
      541 gtcctgcgat acagggtctt tatcagcatt ttcctcggat atatggggta ttattttgtc
      601 cgaaacacca ccagtgtgtt atccggtgtg ctgcacatgt ctgcaaccga gatcggcatt
      661 atctcctgtg ccggtttttt atcctatggt atatcaaagt ttgtttccgg acttatttct
      721 gacagaagta attcaaaagt atttttgtct cttggtttat tcctgtccgg actggtaaat
      781 tttctgatag gatatattcc cgggatcatc acatccgtta cgttattttc aacaatgtat
      841 ttacttaacg gctggatcca aggtatggga tatcctcctg g
//
LOCUS       DAJTRQ010000302          877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000302 DAJTRQ010000000
VERSION     DAJTRQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..566)
                     /gene="virK"
                     /locus_tag="OO332_004244"
     CDS             complement(<1..566)
                     /gene="virK"
                     /locus_tag="OO332_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5944445.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIR"
     gene            complement(571..>877)
                     /gene="capU"
                     /locus_tag="OO332_004245"
     CDS             complement(571..>877)
                     /gene="capU"
                     /locus_tag="OO332_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5944446.1"
                     /translation="ASLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQNNQT
                     GTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          273 a          192 c          168 g          244 t
ORIGIN      
        1 cgaataacat caggtccggt atttttaggg gctccctgta atccgccaat aaaagcaatg
       61 tgtccatttt gagtcagaat gactgaaaat gttaaccggg caataactgt tttattgtaa
      121 aacaatgaca acatcaactc tccttcccgg tcaaaaccac tgggagaaca ctggatatca
      181 aaattctctc catctttccc gatgaattgg actaatgata ccgatttttg tggaaacaaa
      241 aatttagaat atttttctgg caaattttgc acaaagcgat aatgctcaag aatatattgt
      301 ccacgggtcc aggcgcgccc ccctttatgc aaataagggc gatgaattct tgctggcaac
      361 gtggggtgta ttgccagaat atcctcaatg cacggcaagg aacgcagttc ataataatat
      421 cgaatactga caaccggatt tatagaacat cgcaaaatgt acatgaaccg aaattttctg
      481 tgttcccact tccccggtag tgaatctgag gaaaaaataa tccttttaag gaaaccgata
      541 aatgataagt tacttataga aaacatattg ttacttttgc ttcgctaatg tgagtatttt
      601 taacgcagtt ttattaatat caaaccgctc ttctatatcc tgttttgcca ggcgagccat
      661 ctgataaaaa cgcccagggt cattaaaaaa atcattcagg gcgcacatcc atgcgtgctt
      721 attacttgct ggtaacaatg tcccggtctg gttgttctga ataacctcag ggattccacc
      781 aatctgagtg gccactacag gcacagaaaa tgccgatgct tctgccagca ccataccaaa
      841 agattcgttt tctgaaggca gaaccaccag actggca
//
LOCUS       DAJTRQ010000303          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000303 DAJTRQ010000000
VERSION     DAJTRQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(652..>863)
                     /locus_tag="OO332_004246"
     CDS             complement(652..>863)
                     /locus_tag="OO332_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944447.1"
                     /translation="IALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVGPGT
                     TLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT          251 a          160 c          185 g          267 t
ORIGIN      
        1 acttgcgtaa ttattcaggt cagtttggaa cgcactgtat aaacataaat aacctgatac
       61 tgttgtgtgt tttcaaagaa aacacaggta tttttcacat aaaccttccc cttttcatat
      121 tcgaccactt aatcaaaaaa cggttttata cttgttgccc ccactggggt taaatgtgag
      181 acatgtcgca ctttgtgcca tgtgtccttg ctgattttag gcgcggttca ttcattcttt
      241 ctgttggcat aaagtatggt atcggttggg cgaatgaaag ggactcgggt catggtcaga
      301 caatacctga ggcgttaatt cgttgaaaga gattgtgaat gggggaataa ctgagtctta
      361 actgctgtgc gatagcccgg acagataatt gctcgtcgaa tctgagacgt aatgattctg
      421 gcaaataagt gcgaaagccg gtgatatcta gtgttgtttg agagttcatc gttcgcgtcc
      481 attaatacag atggacgcga ttgttgccag acaggataat tttcacaaga catcgcagat
      541 agatgtactt acgataattt tttattttat acaacagatg cccacgaaaa gaattatttt
      601 caatagacac ctgcattaca caaagtagat acatacgcag tcagagtaca cttacttttt
      661 attatttcct gcaatggcga ttacggctat tgacagtaat gaagttatta gtgaaccaag
      721 acctaaagta gtcccgggac caacaccgtt gtcgcttacg gttactgaca ggaaactgat
      781 tgtaccagcg cttattgttt gagtaaaacc tgtaatagat gcggcaaatc ctgtttttga
      841 accatttatt tgtaatgcga taa
//
LOCUS       DAJTRQ010000304          862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000304 DAJTRQ010000000
VERSION     DAJTRQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..545)
                     /locus_tag="OO332_004247"
     CDS             complement(<1..545)
                     /locus_tag="OO332_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5944448.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYA"
     gene            complement(545..>862)
                     /locus_tag="OO332_004248"
     CDS             complement(545..>862)
                     /locus_tag="OO332_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944449.1"
                     /translation="SKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFF
                     VDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPDGVA
                     PPF"
BASE COUNT          207 a          251 c          219 g          185 t
ORIGIN      
        1 gcgtaaaaat gaagattttt ccaggtgtgg cccgacgtca caccaccggc agttgtcgat
       61 aaatggatat gcggatgcca ctgctggtca cgcccccatg tgtggatcac cgtgaatatc
      121 cccggctcca catctgcctg atggcagatt tccagtatca catccgctgc aatgcggctc
      181 atctctgcca gtaaccaccg gttgtggaac accagggacc agtactggca gggaagtgtg
      241 aacacaatat gctgccacgg gcagtcgggg accaggctca gcagatactg tatccactgt
      301 gcgccagcct tcaccccgca gtgcgggcag gagcggcttt tacaccggaa gcagaccttt
      361 tttgtatggc aacagtccgg tgatgaacaa caccactgcg tgtagcccat cagtgtggtc
      421 ccgcaggcca tgatttttgt cactgattca attaccaccg gacggactgc cccttccggt
      481 tgtttctcca gccagttaag ccagcggttt ccctgctgaa aaatatcggc aaaacgggga
      541 agcatcagaa gggcggggcg actccgtccg gccagtgaac cgtgccacac tccgggcagt
      601 acataccgcc ggcgctgata ccggcaagaa tggtcgcaaa ttcccgctcc gtgcagcggg
      661 cgatttccgg atacccttcg tcatcaacac gtacaaacca gaagaccagc tttttgtttc
      721 ccgcatccac aaagaacgga atattcaggt ctgcgcagca ttcaacggca tcgtcaaaac
      781 tatcaaagcg cagaacttct gcgtcttctt cgtcaaaaaa atcatcttcg tgaagcttca
      841 cgacatagcg gggaagtttg ct
//
LOCUS       DAJTRQ010000305          818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000305 DAJTRQ010000000
VERSION     DAJTRQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            157..648
                     /locus_tag="OO332_004249"
     CDS             157..648
                     /locus_tag="OO332_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944450.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
BASE COUNT          211 a          221 c          203 g          183 t
ORIGIN      
        1 cgctagcttg ctagctgtcg tcagcacacc gttagtagcg cgtctactca atacctaatt
       61 ttaaaccttt tgagatgacc gatgcgggga gacgccgcag gactgaacac gcctgtggtc
      121 gaccgatagt ctcaactctc aagaggagta attactatgt tattcaataa ccaagactgg
      181 aaactgtcag tcaccgacat caatctctac gagaatactg tgagtctgga cggtcagccc
      241 tatccgctgt cgctcgctat caagaccctc attccgggct acctgtcagg cctgccgtct
      301 accagccgcg aggcaatgga gttactggaa gctctggctg aagctggcgt gaccattggt
      361 aacttcttca gtaatgacct gatgaccgcc taccaacgcc gccagatgaa taagcgtgtc
      421 gaggctgagc gtatcgctaa agagctggcg tctcagaaag agcgtacccg cgaaatgttc
      481 atgaccgaag acgaatggca gaaagagcta cagcgccgcg aacaggtcaa agctgaacgc
      541 cggacctatg gtgaaaacct ccgcagtgct acccattccg ctggccgctc ccgtgccgct
      601 atcgtcgctg accttgagtc aggcggtaac tggatggaca gcctgtaaaa cacacagtcc
      661 ctgacccgat gctagggact cctcagtttt gaggactcac agtatttaaa ctaatttagg
      721 agattgatta ttatgacgat taaagacgct ttccagttcg gtattgagcc agtacgtatc
      781 accgacaccg acaacattca ggtcaatgaa ggtctgcc
//
LOCUS       DAJTRQ010000306          812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000306 DAJTRQ010000000
VERSION     DAJTRQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.312"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..812
                     /locus_tag="OO332_004250"
                     /pseudo
     CDS             1..812
                     /locus_tag="OO332_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          219 a          200 c          226 g          167 t
ORIGIN      
        1 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
       61 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      121 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      181 ataaccggtt gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tgcggggatt
      241 gtgtggcgaa gggctgcgcc aactctgcgt atccgtgacc cgcataaaga tgaaaagatg
      301 gcagcaatcc ataaagcact ggacgaatgc agcgcagagc atccggtctt ttatgaagat
      361 gaagtggata tccatcttca tcccaaaatc ggtgcggact ggcaactgcg cggacagcaa
      421 aaacgggtgg tcacgccggg acagaatgaa aaatattatc tggccggagc gctgcacagc
      481 gggacaggta aagtcagcta tgtgggcggc aacagcaaaa gttcggcgct gttacatcag
      541 cctgctgaag cggcttaaag cgacataccg tcgggcgaaa accatcacgc tgatcgtgga
      601 caactacatt atccacaaaa gccgggaaac acagagctgg ctgaaggaga acccgaagtt
      661 caggggcatt tatcagccgg tttactcgcc atgggtgaat catgttgaac ggctatggca
      721 ggcacttcac gacacaataa cgcgtaatca tcagtgccgc tcaatgtggc aactgttgaa
      781 aaaagttcgc cattttatgg aaaccgtcag cc
//
LOCUS       DAJTRQ010000307          806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000307 DAJTRQ010000000
VERSION     DAJTRQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(12..374)
                     /locus_tag="OO332_004251"
     CDS             complement(12..374)
                     /locus_tag="OO332_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5944451.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAIRYMLYVGFMGILSAIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(636..>806)
                     /locus_tag="OO332_004252"
     CDS             complement(636..>806)
                     /locus_tag="OO332_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5944452.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNL
                     SQSRNKEGTNDV"
BASE COUNT          240 a          163 c          175 g          228 t
ORIGIN      
        1 aagagatatt ttcatttcgc atccctaaaa acaatgaact ttgaatagac gaaaccgcac
       61 accagactga tggcggagaa ggtgataaga gtaactattg gaggaagtga gcatttatca
      121 gcagcccaac caacaatcgc actcaatatt cccatgaatc ccacgtataa catgtagcgt
      181 atcgctgtag tcgatgcttt gaatgtgaat cttgcattcg cgaagaagct aaagctcaca
      241 gctactacga aacctgtgaa gtttgccagg gcctgactgg tatgcgcggc atagatacat
      301 acaccaaaaa ctacccagtg tataagtgta ttcagcacac ctatagatgt gtactttaca
      361 aataacttta acatttattt aatcaatgag ttctgaaagt catgaagtct atcatccaag
      421 tctcaattga tcgatatttg ctgtgtctga tgagacaaaa ctgagacaca taaggcctcg
      481 caatggcttg caaggcttta catgttttga tttagtggga cgtgtgagcg cagtgttgat
      541 ggggtaatgc tttgaattag aagcggattc ttataattcg taatgcgaag gtcgtaggtt
      601 cgactcctat tatcggcacc atttaaatca ataagttaca catcattagt accttcctta
      661 tttcttgact gggacaaatt tgggaccgat gggttcagaa tcgagtctat ttgccgtgcg
      721 tgttcggtaa ggtgattagg tgcgaggtga gcatatcgac gaaccatttc gatagactcc
      781 cagcctccca tttcctgtaa cactga
//
LOCUS       DAJTRQ010000308          805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000308 DAJTRQ010000000
VERSION     DAJTRQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..455
                     /gene="ospC3"
                     /locus_tag="OO332_004253"
     CDS             <1..455
                     /gene="ospC3"
                     /locus_tag="OO332_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC3"
                     /protein_id="HCR5944453.1"
                     /translation="TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFIN
                     NGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          284 a          134 c          163 g          224 t
ORIGIN      
        1 gtacagataa cttcaaaaaa ataaaactca gagacatatc tttggaggat gcgattaaag
       61 catctaatta tgaagagatt aacaataaag tgactgataa aaaaatggct catcaggccc
      121 ttgcttattc tttaggcaat aaaaaagctg atatagcact ttatttacta tctaaattta
      181 attttacaaa acaagatgtc gcagagatgg agaaaatgaa caacaataga tattgtaatc
      241 tgtatgatgt tgaatactta cttagtaaag atggtgccaa ctataaagtg ctagaatatt
      301 ttatcaataa tggattggtt gatgtaaaca aaaaatttca aaaagcaaat agtggggaca
      361 ctatgcttga taatgcaatg aaaagcaaag attcaaaaat gattgatttt ttattaaaaa
      421 atggagcaat attaggcaaa cgatttgaga tatgacctaa aagctcggcc aaataataat
      481 gtcgtcgatt atcgaagtca tcatgagtga ttctgtcccc acagtgcagc atcagcatga
      541 cagtgaattg tctggcgtgg tccatttaat gagatggaga tgctccctct ttcctaaata
      601 gctgtgccta ataccgctac acttttgcca gcccatgttt gcctccgggg aatgggctga
      661 cggttccttc aaaatccatt gcacgcagtt atgtcggagc gggaacttga ctcctgtctt
      721 tacgggagtg aggtagcctg agtttaacgg acactccttc ctgaaataga atggcatcag
      781 aaggagccaa taatgagcag aaaaa
//
LOCUS       DAJTRQ010000309          803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000309 DAJTRQ010000000
VERSION     DAJTRQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.315"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..803
                     /locus_tag="OO332_004254"
                     /pseudo
     CDS             1..803
                     /locus_tag="OO332_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          200 a          208 c          218 g          177 t
ORIGIN      
        1 cgtctcgata tcaccaccca cagcctttat gcctggataa agaagtacgg tccggattct
       61 tccactaata aagaacagtc agatgctcag gccgagatcc gccgtctcca gaaagagctg
      121 aaacgggtta ccgacgaacg ggacatatta aaaaaagccg cggcgtactt cgcaaagctg
      181 tccgactgag gtacgccttt atccgtgaca actcctgttg ctggcctgtt cgcctgctct
      241 gtcgggtgct ggatgttcat cccagtggtt tttacgcctg gcttcagcag ccgcattcac
      301 aacgccatca ggcagacctg agactgacag gacagattaa acagttctgg ctggaatcgg
      361 gatgcgtcta tggttatcgc aaaatccatc tggatctgcg tgacagcggg caacagtgcg
      421 gagtaaacag agtctggaga ctgatgaaac gtgtcggaat aaaggctcag gtcggatacc
      481 gaagcccgcg ggcacgtaaa ggcgaggcca gtatcgtgtc acccaacagg ctccagcgac
      541 agttcaatcc ggatgctcct gatgagcgtt gggtaacgga cataacctac atcaggaccc
      601 acgaaggctg gctgtatctt gccgttgttg ttgatctgtt ctcacgcaaa attatcggct
      661 ggtccatgca atcccggatg acaaaggaca ttgtcctgaa cgcactgctg atggctgtat
      721 ggcggcgtaa tcccgaaaaa caggtgctgg ttcattcgga tcagggcagt cagtacacaa
      781 gccatgagtg gcagtcgttc ctg
//
LOCUS       DAJTRQ010000310          801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000310 DAJTRQ010000000
VERSION     DAJTRQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..391
                     /locus_tag="OO332_004255"
     CDS             <1..391
                     /locus_tag="OO332_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5944454.1"
                     /translation="MTIKGQKLVLPSWKFALAQMLISSVNWMVMGAIIWLLLGQSVNY
                     FFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGSIIAALLAYRVLYYFI
                     PLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(427..>801)
                     /locus_tag="OO332_004256"
     CDS             complement(427..>801)
                     /locus_tag="OO332_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR5944455.1"
                     /translation="GAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMS
                     ERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIG
                     FFGILAAIAITLVWQRHTRFFH"
BASE COUNT          218 a          185 c          189 g          209 t
ORIGIN      
        1 tatgaccatc aaagggcaaa agctggtgct gccttcatgg aaattcgccc tcgctcagat
       61 gctgatttcc agcgttaact ggatggtaat gggggcgatt atctggctgt tacttggtca
      121 aagcgtgaac tatttctttg tactgggcgt gttactggtt agtagtattg ctggcgtcat
      181 cgtgcatatt ccggcgggga tcggtgtgct ggaagcggtg tttatcgcac tactggctgg
      241 ggagcatacc tcgaaaggga gcattatcgc cgccctgctc gcttatcgcg tcctgtatta
      301 ctttatcccg ctgctgctgg cgctgatctg ttacctgcta ctggaaagcc aagcgaagaa
      361 gctacgggcg aaaaatgaag cggcgatgtg aagtttagtt aagttcttta gtatctgcat
      421 ttacgactaa tgaaaaaaac gcgtatgcct ttgccagaca agcgttatag ctatcgcggc
      481 aagtatccca aagaagccga taaaatagta atagagtgtt aatgcacctg taaaagcgca
      541 acgaatcgga ctccacaact cttcactatg gcattttcct gccagttcaa ggagcttatt
      601 tcgtccatgc gagataatgc ctggccataa cacaatcatt aagcaactta ttacccaaat
      661 gggccgttca ctcattaatg ccgcattcac gatgattacc agggccaacc ctgtcaacgt
      721 catcataatg ataaaacggt gagaaccagg atcaacatta aataataaac cggccagcat
      781 actgattaaa aacatcgcgc c
//
LOCUS       DAJTRQ010000311          795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000311 DAJTRQ010000000
VERSION     DAJTRQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..720
                     /locus_tag="OO332_004257"
     CDS             <1..720
                     /locus_tag="OO332_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5944456.1"
                     /translation="AICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKTTNQ
                     IHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELES
                     ELSQSINADDTAQRIMTIPSVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYST
                     GGKSTLLGISKRGDKNLRRLLVQCARSFMIQLERQHGKLAEWVREQLNKKHSNVVACA
                     LANKLARIAWAITTQQKEYQA"
BASE COUNT          227 a          185 c          192 g          191 t
ORIGIN      
        1 gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag aactgagacc
       61 cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga taaggtgaaa
      121 acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc gacaggggat
      181 gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga atatctgagc
      241 cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat agcagagctg
      301 gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat tatgacaatt
      361 cctagtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga cgggaaacag
      421 ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca gtatagcact
      481 ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt acgccggtta
      541 ctggttcagt gtgcccgctc attcatgata cagcttgaac gccagcatgg aaaacttgct
      601 gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg cgctctggca
      661 aataaactgg cccgaattgc ctgggcaatc actactcagc aaaaagaata tcaggcctga
      721 tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac aagttaccgt
      781 ctggttttgc gaaga
//
LOCUS       DAJTRQ010000312          787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000312 DAJTRQ010000000
VERSION     DAJTRQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..787
                     /locus_tag="OO332_004258"
                     /pseudo
     CDS             1..787
                     /locus_tag="OO332_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          209 a          203 c          179 g          196 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccat
//
LOCUS       DAJTRQ010000313          774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000313 DAJTRQ010000000
VERSION     DAJTRQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..450)
                     /gene="speB"
                     /locus_tag="OO332_004259"
     CDS             complement(<1..450)
                     /gene="speB"
                     /locus_tag="OO332_004259"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5944457.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDA"
     gene            complement(586..>774)
                     /locus_tag="OO332_004260"
     CDS             complement(586..>774)
                     /locus_tag="OO332_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944458.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT          193 a          213 c          213 g          155 t
ORIGIN      
        1 ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg
       61 cagcgtaaca aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt
      121 ctcggcgtgc gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag
      181 atcgccgcag tccacgacgt tcagacgctc acgcatgtcg aaattccacg ggaagcggtt
      241 gtgttcccag gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc
      301 cgcacgacca gaagtggcca tatcgaacgg cacgccagta atcacccagt ctgcatcgct
      361 gtcatacggc tggaagttca tcggcaggcg taaaaaacca aaggcattgg aaaccagtga
      421 gttatcgtat tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa
      481 aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt
      541 tgtgctaatt atcgccttaa atgggcgagg catcaagcga ggttgctatt tccgacacgg
      601 tggcggcgca taaccattgt tatctgccgg aaaggcttgt ccagagcacg gcggcagcgc
      661 gttttgcgcc gggccttgta attcaaaggc ggcaaagccg attaccccgg tattacgcac
      721 atcgccttgc gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaa
//
LOCUS       DAJTRQ010000314          769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000314 DAJTRQ010000000
VERSION     DAJTRQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            440..>769
                     /locus_tag="OO332_004261"
     CDS             440..>769
                     /locus_tag="OO332_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5944459.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADN"
BASE COUNT          207 a          135 c          189 g          238 t
ORIGIN      
        1 cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa tcagccggag
       61 atgattttac ataattgcta cggaattatt caatacagga attgcttgcg tctgcatgga
      121 ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg taactggttg
      181 aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa atttattcaa
      241 cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcat gttcggtctc
      301 tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac
      361 acactctact gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta
      421 gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca
      481 tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg
      541 caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag tgggagacgt
      601 tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggttcg
      661 tgcggtaaga ggcaatcatg aacagatggc gatggatgcg ctggcattcc agcagatgtc
      721 tttgtggttg atgaatggcg gcgactggtt tattgcgctg gcagataat
//
LOCUS       DAJTRQ010000315          750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000315 DAJTRQ010000000
VERSION     DAJTRQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..363)
                     /locus_tag="OO332_004262"
     CDS             complement(<1..363)
                     /locus_tag="OO332_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944460.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(430..>750)
                     /locus_tag="OO332_004263"
     CDS             complement(430..>750)
                     /locus_tag="OO332_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HCR5944461.1"
                     /translation="RIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEY
                     MGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLPVARLEDGT
                     LLNW"
BASE COUNT          238 a          190 c          157 g          165 t
ORIGIN      
        1 cagaccgata cgcattaaaa taaagtggct aaaagaatta accattcctg ttaatgcctg
       61 cattaatgac cagaatatta agcctgcgcc taaaactatt cgtgggccaa agcggtctag
      121 caaaataccg gaaggtagtt gtgaaaaacc ataagacaga gaaaatgcag aaagcaaaac
      181 accaaactct gtcgccgata atcctaactc gccacgaatc gcttcgcctg ccacgctcaa
      241 tgacgaacgg tcgagaaaat taacgatccc cgccataaat aataaaacca gggtcactgt
      301 ctgaatacga cgaatccgtt gcggtttatt acccgcaaca ctggttgaca catcaacgtc
      361 catttttagc tccttgagcg gaattaatcg atttgatgac tattcagaaa ataattaagc
      421 gaaccgttat taccagttca acaatgtgcc atcttccagt cgtgcgacgg ggagatactc
      481 cgggtcatac ggatatttcg ccgccagttt ctcatcgaaa gaaataccca gccccggttc
      541 atcgcctggg tgcatataac cttcctcgaa gcgccagctg tgcgggaaaa cttcaagcat
      601 ttgttcggaa tagcccatgt actcctgaac gccaaaattt ggtacccata ggtcaaaatg
      661 cagcgccgcc gcatgacaaa caggcgataa atctgaaggg ccatgcgaac cggtacgtac
      721 ctgataaagc gatgcaaaat cggcaatccg
//
LOCUS       DAJTRQ010000316          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000316 DAJTRQ010000000
VERSION     DAJTRQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>747)
                     /locus_tag="OO332_004264"
     CDS             complement(<1..>747)
                     /locus_tag="OO332_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR5944462.1"
                     /translation="QATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVW
                     CTSLPGNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIR
                     GALNVPLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFS
                     FYRLIDKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVN
                     GREGTLRGVEVLARWKQPHGGYISPAAFIP"
BASE COUNT          202 a          175 c          178 g          192 t
ORIGIN      
        1 gtggaataaa tgccgcgggt gaaatatatc caccgtgagg ttgtttccag cgggctaaca
       61 cctcaactcc ccgcaatgtc ccttcccgac catttaccac aggttggtaa aaagggacga
      121 tctccccatt atttattgcc cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat
      181 atctatcaag caggaaggca gcggcgcagg cgattaacag aataaatatt aaaatcccaa
      241 agcctttatc gataagtctg tagaagctaa agagtggcgg cgggttaaag ataatagtga
      301 aatgatattt tgtggagtga accttctcgg tatagggata atgtccatta agcgtcatca
      361 catcacccgt tggtccaata atgtcatccg ccacgcgtaa tacatacctt acccctttca
      421 agggtacatt aagcgccccg cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat
      481 ctgcaaattg gctctgatag agcaggatag gtaatctatt aacggtgtcg attgccggag
      541 ccaacagtaa attactgtcc gggacaacag gaatacgaga cagcaggacc cgattcccag
      601 gcagggatgt acaccagact attccctgtt tgagaatgat gattgtgcgc aggtgaggtt
      661 tcagagctgc ttcagtgcca agctgatatt gctcctcgag gtcgcattcc ttcccggcaa
      721 tgttaaccgc tgtttgagtc gcctgtg
//
LOCUS       DAJTRQ010000317          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000317 DAJTRQ010000000
VERSION     DAJTRQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>743
                     /locus_tag="OO332_004265"
     CDS             <1..>743
                     /locus_tag="OO332_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCR5944463.1"
                     /translation="NVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIG
                     FIVTFSVGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAF
                     GFKLNETWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGA
                     VLIALGILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHE
                     RDAFWEMKEKGEAYKKPDHYEEIHMPKNS"
BASE COUNT          149 a          198 c          203 g          193 t
ORIGIN      
        1 gaacgtaaac gccttctttg gtatcaccac aatgattatc gccatcccga ccggggtgaa
       61 gatcttcaac tggctgttca ccatgtatca gggccgcatc gtgttccatt ctgcgatgct
      121 gtggactatc ggttttatcg tcaccttctc ggtgggcggt atgactggcg tgctgctggc
      181 agtaccgggc gcggacttcg ttctgcataa cagcctgttc ctgattgcgc acttccataa
      241 cgtgatcatc ggcggcgtgg tcttcggctg cttcgcaggg atgacctact ggtggcctaa
      301 agcgttcggt ttcaaactga acgaaacctg gggtaaacgc gcgttctggt tttggatcat
      361 cggcttcttc gttgccttta tgccgctgta tgcgctgggc ttcatgggca tgacccgtcg
      421 tttgagccag cagattgacc cgcagttcca caccatgctg atgattgcag ccagcggtgc
      481 agtactgatt gcgctgggta ttctctgcct cgttattcag atgtacgttt ctattcgcga
      541 ccgcgaccag aaccgtgacc tgactggcga cccgtggggt ggccgtacgc tggagtgggc
      601 aacctcttcc ccgcctccgt tctataactt tgccgtagtg ccgcacgttc acgaacgtga
      661 tgcattctgg gaaatgaaag agaaaggcga agcgtataaa aagcctgacc actatgaaga
      721 aattcatatg ccgaaaaaca gcg
//
LOCUS       DAJTRQ010000318          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000318 DAJTRQ010000000
VERSION     DAJTRQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..286
                     /locus_tag="OO332_004266"
     CDS             <1..286
                     /locus_tag="OO332_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR5944464.1"
                     /translation="IARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVI
                     VTHNMQQAARCSDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            301..>733
                     /gene="phoU"
                     /locus_tag="OO332_004267"
     CDS             301..>733
                     /gene="phoU"
                     /locus_tag="OO332_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5944465.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLD"
BASE COUNT          181 a          193 c          209 g          150 t
ORIGIN      
        1 tattgcgcgt ggtatcgcca ttcgcccgga agtgctgctg ctcgacgaac cgtgttcggc
       61 gctcgacccc atctctaccg ggcgtattga agagctgatc accgaactga agcaggatta
      121 caccgtggtg atcgttaccc acaacatgca gcaggctgcg cgttgttccg accacacggc
      181 atttatgtac ctgggcgaat tgattgagtt cagcaacacg gacgatctgt tcaccaagcc
      241 agcgaagaaa caaacagaag actacatcac cggtcgttac ggttgattca ggagtgcgtt
      301 atggacagtc tcaatcttaa taaacatatt tccggccagt tcaacgccga actggaaagt
      361 atccgcacgc aggtgatgac catgggcggc atggtggagc agcagctttc tgatgcaatc
      421 accgcgatgc ataaccagga cagcgatctg gcgaagcgcg tgatcgaagg cgacaagaac
      481 gtcaacatga tggaagtggc gatcgatgaa gcctgcgtgc gcattatcgc caaacgtcag
      541 ccgacggcga gcgacctgcg actggttatg gtgatcagta aaaccattgc cgagctggag
      601 cgtattggcg acgtggcgga caaaatctgc cgtactgcgc tggagaaatt ctcccagcag
      661 catcagccgt tgctggtaag tctggagtcg ctgggccgtc ataccatcca gatgctgcac
      721 gacgtgctgg acg
//
LOCUS       DAJTRQ010000319          725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000319 DAJTRQ010000000
VERSION     DAJTRQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..452
                     /locus_tag="OO332_004268"
     CDS             <1..452
                     /locus_tag="OO332_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944466.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            449..>725
                     /gene="tnpB"
                     /locus_tag="OO332_004269"
     CDS             449..>725
                     /gene="tnpB"
                     /locus_tag="OO332_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5944467.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT          187 a          185 c          209 g          144 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctctcac tccggaactg ttacgggcgc
      421 taatccgcga aatgaaaggg ggtatccgat gataacgctg ccgaccggta ccaaaatctg
      481 gatcatcgct ggcatcacag atatgcgttg tggcttcaat ggcctggctt cgaaggtgca
      541 gaacacgctg aaagatgacc cgttctccgg gcatatcttc gtcttccggg gccgcagtgg
      601 caaaatggtg aaaatactgt gggccgatcg tgacgggtta tgcctgttcg ccaaacgcct
      661 ggaacggggc cgcttcgtct ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc
      721 tcagt
//
LOCUS       DAJTRQ010000320          719 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000320 DAJTRQ010000000
VERSION     DAJTRQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            202..>719
                     /locus_tag="OO332_004270"
     CDS             202..>719
                     /locus_tag="OO332_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944468.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTV"
BASE COUNT          175 a          200 c          173 g          171 t
ORIGIN      
        1 agctgaacgc cggacctatg gtgaaaacct ccgcagcgct actcattccg ctggtcgctc
       61 acgtgccgct atcgtcgctg accttgagtc aggcggtaac tggatggaca gcttgtaagc
      121 aagcgtccct catctgagaa cacacggtcc ctgactgcct gttagggact tcaagtattt
      181 gactcaagga gattaattat aatgaatatg actaaaaacg ctttccagtt tggcattgag
      241 cctgttcata tcaccgacaa cgacaacctt caggttaatg agggccttcc gactaacgcc
      301 gaccctcagg tctatgccct ccagttagct aagactgtta aggctatgct gaacggtgta
      361 cttaaagacg ctcaagataa catcccgttc cctgtagaag tcctgccgac ccgtaacagc
      421 ttaccgactc caatcattgc tcacactctg gctgaccgct ctgtgctggt ccctgtccgt
      481 ggtggtaagc gacctgaagt tgtgaccgct ccgtctggcg ctgaaatcac cgttgagccg
      541 attgaacagg cgatcctcgt gtctcaccaa acaaagcttt gggaccagaa gtcgaccact
      601 ggtttcactc aggggacgct ccagcaggac gcactgaaca tctgcgataa cgtgattcgt
      661 accattaact ctaagatggt tgacgtgctg gagtcctcaa agctgctgaa gaccgtaga
//
LOCUS       DAJTRQ010000321          715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000321 DAJTRQ010000000
VERSION     DAJTRQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..454
                     /locus_tag="OO332_004271"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO332_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="OO332_004272"
     CDS             514..621
                     /locus_tag="OO332_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5944469.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..>715)
                     /locus_tag="OO332_004273"
     CDS             complement(602..>715)
                     /locus_tag="OO332_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5944470.1"
                     /translation="GFNTAYVIQGALLVALAAIIADRLFERLVQALSQHAK"
BASE COUNT          166 a          200 c          176 g          173 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatcc
//
LOCUS       DAJTRQ010000322          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000322 DAJTRQ010000000
VERSION     DAJTRQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..182
                     /locus_tag="OO332_004274"
     CDS             <1..182
                     /locus_tag="OO332_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5944471.1"
                     /translation="PQHLFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYM
                     AETIAHHQVDIDLED"
     gene            188..>710
                     /locus_tag="OO332_004275"
     CDS             188..>710
                     /locus_tag="OO332_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5944472.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQ"
BASE COUNT          195 a          161 c          184 g          170 t
ORIGIN      
        1 tgcctcagca cctgtttgaa tcgctctgca tcgaccattt gcaacgccac ggtgccaata
       61 aacaggcaat cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac
      121 acatccggta catggctgaa accattgccc accaccaggt tgatattgat ttggaggact
      181 aaaatctatg gcgctttttc aacgagcaat aaacacacag gcctatctaa aagcaggaat
      241 tatggggttt gctggcgacg gcaaaaccta cacagcaagc gaactggcta ttggtctggt
      301 aatgcttatg cggcaacggg gaattgaagc tggtaaccgc ccggtaatgt ttctggacac
      361 agaaacaggc tctgactggg ttaaaccccg ctttgatgcg gaaaatatcg aattatttac
      421 ggcaaaaacc cgctcgtttg ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg
      481 ttccgtaatg atcatcgact ccatcagtca cttctggacc ggattatgcg acgagtatgc
      541 cagacgccga aaccgtaaac gaggactgga attttcagac tgggcgtggc tcaaacagga
      601 gtggcgacgc tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg
      661 tgcaggatat gagtatgact ttttcgaagg tgatgatggc aaacgccagt
//
LOCUS       DAJTRQ010000323          708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000323 DAJTRQ010000000
VERSION     DAJTRQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>708
                     /locus_tag="OO332_004276"
     CDS             <1..>708
                     /locus_tag="OO332_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944473.1"
                     /translation="LVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEAARTLPHALRT
                     LKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLD
                     LLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVV
                     LPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAF
                     ASAAHLAAYAGLAPVTR"
BASE COUNT          139 a          244 c          185 g          140 t
ORIGIN      
        1 tccttgtcgg atacctccct ggactggcca tgcgccgcat agccgactta cacgccggtg
       61 aagctaaaac tgatgctcgt gacgctgcca tcattgccga agctgcccgt accctgcctc
      121 acgcgctacg cacgctgaaa ctggctgacg agcaaatcgc cgaactctcc atgctctgcg
      181 gcttcgatga tgatcttgcc gcacagacaa cgcaggccag caaccgtatc cgcggccttc
      241 tgacccagat acatccggca ctggagcgcg ttctcggtcc gagacttgag cacccggcgg
      301 tactcgatct tctccagcga tatccctcac cagaaaaact cgcttcgctg ggtgagaaga
      361 agctggcagc ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt gcagcagaca
      421 tagctcaggc actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc gctgccgtag
      481 tactgccacg tctggcactc cagctcatca cgctgcgtaa gcaaagagac gaggtggcgc
      541 ttgaggtaga acagcgagtt cttgctcacc ctctttaccc ggtcctgacc agtatgcccg
      601 gagtcggtgt caggaccgca gccagactcc tcaccgaggt cgcctgccgc gccttcgcct
      661 ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt aactcggc
//
LOCUS       DAJTRQ010000324          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000324 DAJTRQ010000000
VERSION     DAJTRQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.330"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            15..>702
                     /gene="rluF"
                     /locus_tag="OO332_004277"
     CDS             15..>702
                     /gene="rluF"
                     /locus_tag="OO332_004277"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5944474.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDEL"
BASE COUNT          186 a          163 c          198 g          155 t
ORIGIN      
        1 ttacaggaga atttatgctg cccgactcat cagtccgttt aaataaatac atcagcgaaa
       61 gcggaatttg ctcacgccgc gaagcggatc gctatatcga gcaaggcaat gtgttcctta
      121 atggcaagcg agccaccatt ggcgatcagg tgaaacccgg cgacattgtg aaagtaaacg
      181 gtcagttgat tgagcctcgg gaagccgaag atttggtact tatcgccctg aacaagcccg
      241 tcggtattgt cagcaccacc gaagatggcg agcgcgataa cattgtcgat ttcgttaacc
      301 acagcaaacg cgtgttcccg attggccgcc tggataaaga ctcccagggg ctgattttcc
      361 tcaccaatca cggcgatctg gtgaataaga tcctccgtgc gggtaacgat catgagaaag
      421 agtatctggt gacggtcgat aaaccgatta ccgatgaatt tattcgtggt atgggggcgg
      481 gggtgccgat cctcgggacg gtgacgaaaa agtgcaaagt caaaaaagaa gcgccgtttg
      541 tcttccgcat taccctagtg caggggctga accgtcagat ccggcgcatg tgcgagcatt
      601 tcggctatga agtgaaaaag ctggaacgta cgcgcattat gaacgtcagc ttaagcggca
      661 ttccgctggg ggagtggcgc gatttaaccg acgatgagtt aa
//
LOCUS       DAJTRQ010000325          698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000325 DAJTRQ010000000
VERSION     DAJTRQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..269)
                     /locus_tag="OO332_004278"
     CDS             complement(<1..269)
                     /locus_tag="OO332_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944475.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRH"
     gene            complement(271..549)
                     /locus_tag="OO332_004279"
     CDS             complement(271..549)
                     /locus_tag="OO332_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944476.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT          167 a          181 c          204 g          146 t
ORIGIN      
        1 atatggcgca acagccagtc agacaatgcg ccagcaccac cagcagcacg aatcacccgt
       61 gcgttactga aaaacggcag caatgttttg tcttccacta gcggctggcg aacggcagga
      121 acgaccccgg acggcagatt acgcatgctt ttcggttccg gctccaccag tacccgggta
      181 ttgtggaata ccggcatgga ttcacggcct ggcttaagga ccaccagccc gagttccgga
      241 accagaacag gtcgaagtaa tacccgcaca ttacctccag atccgttgct ggaatgtgca
      301 ggacggacgc ggtggccgtt cggaataagg gagcctgacg aagattatcc agtgacgata
      361 atcgagactg agggctttct taatctcgta tccgtgtctg cggtagcgct gaatcagcca
      421 ttcagcctgt tcttcggtgc aggagtcgtg ctgataccag tcagatttga atgcatgaga
      481 acgccgcccg tgcctgctgg caggggcggc agagttatcc gaattgtaaa atttggtatc
      541 gtgcgccatc tgttttctct gctggcgcag caggtgccag ttgttcaggc tgacggatgg
      601 attgtaaacc agaacgacca gaaaaaacaa aacccgccga agcgggttaa gtgcgggtgc
      661 gttgaggatg cctgacacat cagcggtggc gagggatt
//
LOCUS       DAJTRQ010000326          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000326 DAJTRQ010000000
VERSION     DAJTRQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            36..>692
                     /gene="istA"
                     /locus_tag="OO332_004280"
     CDS             36..>692
                     /gene="istA"
                     /locus_tag="OO332_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR5944477.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 ataatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DAJTRQ010000327          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000327 DAJTRQ010000000
VERSION     DAJTRQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..216
                     /locus_tag="OO332_004281"
     CDS             <1..216
                     /locus_tag="OO332_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR5944478.1"
                     /translation="VYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSEYRKRY
                     ALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            281..>691
                     /locus_tag="OO332_004282"
     CDS             281..>691
                     /locus_tag="OO332_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5944479.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPH"
BASE COUNT          215 a          126 c          150 g          200 t
ORIGIN      
        1 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
       61 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgagtac
      121 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
      181 tatcctgtac actcagaaca tcagaattta aattaaggtt tgatgtattc tctgaataaa
      241 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      301 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      361 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      421 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      481 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      541 gtttgtcaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
      601 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgacacaa
      661 taatagaaat gtgatttttc tggttcccca t
//
LOCUS       DAJTRQ010000328          690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000328 DAJTRQ010000000
VERSION     DAJTRQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.334"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..672
                     /locus_tag="OO332_004283"
     rRNA            <1..672
                     /locus_tag="OO332_004283"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          153 c          226 g          149 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 aggcttaacc ttacaacgcc gaagatgttt
//
LOCUS       DAJTRQ010000329          673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000329 DAJTRQ010000000
VERSION     DAJTRQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            307..673
                     /locus_tag="OO332_004284"
                     /pseudo
     CDS             307..673
                     /locus_tag="OO332_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          183 a          154 c          145 g          191 t
ORIGIN      
        1 acgccctgtg gttgaaacgt gagcatattg ttttcagatg ctgttgtctt gcttcagagt
       61 gatctgtcct ccggatttac gcaaagttat ccacaggcac tgtgcattta aaaaacttat
      121 cttttcttct tgattttaaa aactcagatc gtcatatcat tatgataaac ataataaaaa
      181 actctaaaca tgagaaggtt tttaacgtaa ggtgagagga tttttaagtg tatccataga
      241 ttaaaaatga caatgcctcc aattagttga attaggggtt ataatacccg cttttaaggt
      301 tctttcatgg tcacagttaa ctttcattgc ccccgttgcc agtccgttca ggtttaccgt
      361 cacgggcaga acccaaaagg acatgatcga ttccgttgcc gtgactgtca ccgggtgttt
      421 cagctcactt actgttacga ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg
      481 gctttcaacg gtgccggagt tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc
      541 gtcatacgga cattaaaaag ctcccgcccc ggcggataac atctgcgcca gtcgctcacg
      601 ctgatgttgc ccttatctgt gaacttgatg ggcagtggag cttcgttggc agcaaggcgc
      661 aacaacactg tca
//
LOCUS       DAJTRQ010000330          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000330 DAJTRQ010000000
VERSION     DAJTRQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..161
                     /locus_tag="OO332_004285"
     CDS             <1..161
                     /locus_tag="OO332_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5944480.1"
                     /translation="FGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNRP
                     KYIIKDKK"
BASE COUNT          216 a          106 c           99 g          251 t
ORIGIN      
        1 tctttggtaa cccagtacgc gggtatccct ccctgcttgt atcaatactt ttcttgggtg
       61 gcgtgcaact gatcgggatt ggtgttctcg gagaatatat aggcagaata tatattgaag
      121 taaaaaatag acctaaatat attattaagg ataagaaatg attaaatcaa attacatatg
      181 ctttcttttg ctttttttat tggtttaact attcttcatt taaatcaata tacactagta
      241 tcagatgaca tttggttttc cgagaaatca atatcagaac cggatcacat caagtggctc
      301 atagatagat atttcacatg gtcatcacga acatagattg aatatgcatt aataaatctt
      361 attaatcatt tttatgcttg gtctttaatt aatgcagcaa tgttttcatc attaattatt
      421 ggcatttgct ctatctttca gagaaaaaac aacctttact ataaaataaa cacttcaatc
      481 atgtcattat tactgattgt tatgatgcca agcgatgttt ttttcaatgc cgcgatttgg
      541 ataacaggat cattcaacta tctgtggcca agtgctattg catttattgg ttattctcta
      601 cttataaaaa aaatcaatat ggaaaaccac gagaaaactc aatctctaat tttttatttt
      661 ttatttttta tt
//
LOCUS       DAJTRQ010000331          661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000331 DAJTRQ010000000
VERSION     DAJTRQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(105..235)
                     /locus_tag="OO332_004286"
                     /pseudo
     CDS             complement(105..235)
                     /locus_tag="OO332_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(233..328)
                     /locus_tag="OO332_004287"
                     /pseudo
     CDS             complement(233..328)
                     /locus_tag="OO332_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          189 a          123 c          159 g          190 t
ORIGIN      
        1 cttccttatg ttatattttg gtaaaaaatt tattttttta cattatagtc ggctaaatga
       61 agtttactgt aaaaaaacga tactttttcg ggtggcgctt ccaatcagta gaatatgtgt
      121 ttttcaatga aggagccgat gacttttcgt ggatttccac ggaacgcgag aagcagattg
      181 ttttacgtgc cagccgcttg atgcgggtgc ggagggtcag gttgttacgt tcaagatata
      241 gcatgtgctg gatgaagatc ccttttctgg tcacctgttt atctttcgtg gccgacgggg
      301 tgacactgtt aaaatgtaat gaacgcatcc cacacaatta aacacggagt agtatgatgg
      361 ttaaaagggt gaaactggca ggtgtggagc ctcgcagctt tccggcatca tactgcccaa
      421 tacgggatgc ataaagcgtc ctcagactgg agactccaca aatgaaatat acaccggttg
      481 gcgttgatat ggcaaaacat gtcattcaga ttcacttcat caatgagcac atgtcagcgc
      541 cagtgatata agacggtaat ggggatgcgg acgatttcct ggactctcag aagatgaaac
      601 atgtttactg aagaacaaaa aattcgcgct attgagcttt atttcaaata tggtaagaag
      661 c
//
LOCUS       DAJTRQ010000332          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000332 DAJTRQ010000000
VERSION     DAJTRQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            72..>657
                     /gene="ospG"
                     /locus_tag="OO332_004288"
     CDS             72..>657
                     /gene="ospG"
                     /locus_tag="OO332_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5944481.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKY"
BASE COUNT          214 a          103 c          130 g          210 t
ORIGIN      
        1 caaggtagcg tgtaacgtta ttttgtgtga aaatctcttg tgatttttaa atatcgtaac
       61 aaaggtatga catgaaaata acatctacca ttattcaaac accttttcca tttgagaata
      121 ataattctca tgctggcata gtaacggagc ccattctcgg taagttaata ggtcaggggt
      181 cgacagcaga aatctttgaa gatgtgaatg attcatctgc tttgtataaa aagtatgatc
      241 ttattggcaa ccagtacaat gagattctgg aaatggcttg gcaagaatct gagcttttta
      301 atgcttttta tggcgatgaa gcatccgttg ttatacagta tggcggagat gtgtacctcc
      361 gaatgctgcg cgtgcctggg actcccctta gtgacattga tacagctgat atccctgata
      421 atatagagag cctttatcta cagttgatat gtaaattgaa tgagttgagt ataatccatt
      481 acgatcttaa tacaggtaat atgctgtatg ataaagaaag tgaaagttta ttcccaatag
      541 attttcgcaa tatttatgct gaatattacg ctgcaaccaa aaaagataaa gagattatcg
      601 accgacgatt acaaatgcgt acaaatgatt tttattcgtt attaaacagg aaatatt
//
LOCUS       DAJTRQ010000333          650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000333 DAJTRQ010000000
VERSION     DAJTRQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            27..554
                     /gene="hydN"
                     /locus_tag="OO332_004289"
     CDS             27..554
                     /gene="hydN"
                     /locus_tag="OO332_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5944482.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT          156 a          148 c          186 g          160 t
ORIGIN      
        1 attaactgtc attgctggag aatttgatga accgtttcat cattgctgac gcgagtaaat
       61 gtattggttg ccgtacctgt gaagtggcgt gcgtggtttc tcatcaggaa aatcaggact
      121 gtgcatcgct gaccccggaa acatttttac cgcgtatcca tgtcattaaa ggtgtgaaca
      181 tttccacggc gacagtttgc cgtcagtgtg aagatgcacc gtgcgctaac gtctgcccga
      241 atggtgctat cagctgtgat aaagggtttg ttcatgtgat gcaggaacgt tgcattggtt
      301 gcaaaacctg cgtggtggct tgcccgtatg gcgcgatgga agtggtggta cgtcctgtga
      361 ttcgcaacag cggcgcgggt ctgaatgtac gggctgacaa agccgaagcc aataaatgcg
      421 acctgtgcaa ccatcgtgaa gacggcccgg cgtgtatggc ggcttgcccg acccatgcgc
      481 tgatttgtgt cgatcgtaat aaacttgaac aactgagcgc agaaaaacgc cgccgtacgg
      541 cgctgatgtt ctaaaaatct tacagaactg ttctggcatc aaaggaattc ccggaaatat
      601 tcgtagaata cggagcgggc ggtatcgccg acctgagact gccggatacg
//
LOCUS       DAJTRQ010000334          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000334 DAJTRQ010000000
VERSION     DAJTRQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(142..648)
                     /locus_tag="OO332_004290"
     CDS             complement(142..648)
                     /locus_tag="OO332_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5944483.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          171 a          158 c          144 g          176 t
ORIGIN      
        1 cctgtgccat ttttcaggca caaaaaaacc cgcataaagc gggttctttc aagtgtccat
       61 gtctgctatt cgcctcgcga tacagctttg cgaagcttac cggaattgaa gcagtttatg
      121 tgcaaaaatg caagaacttt tttaaagctg catcaacctt tccaccagtt tatctctgcg
      181 aacaacaaac caaccattgg ctctcgccag ttccagccat gactcaaggg aaataacaat
      241 atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat aacagagaac
      301 tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcccttt gactgaaata
      361 gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca ttttggcgtg
      421 gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg aaactggatt
      481 ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa ctttgaaaaa
      541 gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa tacgcaccgg
      601 ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaatc
//
LOCUS       DAJTRQ010000335          640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000335 DAJTRQ010000000
VERSION     DAJTRQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..480)
                     /locus_tag="OO332_004291"
     CDS             complement(<1..480)
                     /locus_tag="OO332_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5944484.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     "
BASE COUNT          173 a          135 c          144 g          188 t
ORIGIN      
        1 ttgcagataa ggagagagtt caatctggtt ggtagcgatg ttttcagcgc caacagcagc
       61 aatcgctttt tccatcaatg ggatcgtgaa gttggaaata ccgatctcac gcgtcagccc
      121 ttgttttttg gcttccagca gcgccttcat aaactcttca acagagactt catcgtttgg
      181 tgacggccag tggattagcg tcagatcaac ataatcggta tgcaattttt gcaggctctc
      241 tttcagactc gggatcaatt tgtctttgct gagattttca atccagattt tagtggtgat
      301 gtagagttca tgacgtggca cgccactttc tgcaatcgcc tgacctactg cggcttcgtt
      361 atcatagatt tgtgcggtat caattgcgcg ataaccaagt tcaagcgccg ttttcacaga
      421 tgaaataaca acgtcgtctt tcagacggaa agtacctaaa ccaaatgcag ggatagccat
      481 aatatgcctc ttttaatgct attttcgtta actgaggtga ttatgggctt cagactgttg
      541 cggaaaaaga gcgtaaaacg cagaggattt ttgcgattct agcaataata gatatccaga
      601 cgcaaaaaaa catcgagcgt taactcgagg cttcttaata
//
LOCUS       DAJTRQ010000336          637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000336 DAJTRQ010000000
VERSION     DAJTRQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.342"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..254
                     /locus_tag="OO332_004292"
     CDS             <1..254
                     /locus_tag="OO332_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5944485.1"
                     /translation="DEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKE
                     KLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            278..>637
                     /locus_tag="OO332_004293"
     CDS             278..>637
                     /locus_tag="OO332_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5944486.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAA"
BASE COUNT          171 a          141 c          180 g          145 t
ORIGIN      
        1 atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat gcccttctgg
       61 ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg ttaatcagcg
      121 acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg caggcgatgg
      181 atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc gcctctccgg
      241 aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga tatcaggtgc
      301 gctgactgat ggcgcaggta tccccatgtc cggataccat attattctga aatcccgggt
      361 aaacaccccg gaagtggtga tgaacactgt tgctgatgtg atgacaggaa acgatggtga
      421 atactgtttc catgcgcgga ctggaaaata tggtgtgtat ctgaaacagg actggcgcaa
      481 cgagtacaac gttggcgaca ttgctgtata tgaggactca aagcccggca cgctgaatga
      541 ctttctgatt gctcctgatg agggcgacct gaaaccggat gtcgtcaaac gctttgagga
      601 aatggtggcg caggcgcagc agagcgccgg ggccgca
//
LOCUS       DAJTRQ010000337          631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000337 DAJTRQ010000000
VERSION     DAJTRQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.343"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            84..>631
                     /locus_tag="OO332_004294"
     CDS             84..>631
                     /locus_tag="OO332_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR5944487.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     VLMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCP"
BASE COUNT          133 a          183 c          180 g          135 t
ORIGIN      
        1 ggggaataca gccatcagtt gcttgccccg gctgacaata tctccattgc aaaaaatgaa
       61 ctggcggttc tggggacgat ttcatggacg tgacaaacga tgattacatc cgtctgttgt
      121 cggcactgtt gccccccggt ccggcgtggt cagccagaga tccggcgatt gccggtgcgg
      181 caccgtcatt aacccgcgtt catcagcgtg cggatgtcct gatgcgggag ctggatccgc
      241 gcaccaccac tgaactgata aaccgctggg agcgtctgtg cggtctgccg gatgaatgta
      301 ttcccgcagg gacacagacc cttcgccagc gtcagcaacg actggatgcg aaggttaacc
      361 tggcgggcgg catcaatgag gatttttacc ttgcacagct tgctgccctg ggcagaccag
      421 acgccaccat cacgcgatac gacaaaagca cgttcacctg ctcatctgcc tgtactgacg
      481 cggtgaatgc gccggaatgg cggtattact ggcaggtcaa catgccagtc gccaccaaca
      541 ccacctggat gacatgtggc gatccctgtg attccgcgct gcgtatctgg ggtgacaccg
      601 ttgtcgagtg tgtgcttaac aaactctgtc c
//
LOCUS       DAJTRQ010000338          614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000338 DAJTRQ010000000
VERSION     DAJTRQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.344"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            67..447
                     /locus_tag="OO332_004295"
     CDS             67..447
                     /locus_tag="OO332_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5944488.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(488..>614)
                     /locus_tag="OO332_004296"
     CDS             complement(488..>614)
                     /locus_tag="OO332_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR5944489.1"
                     /translation="YGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT          188 a          114 c          121 g          191 t
ORIGIN      
        1 tttccacttt cagtatgcta atgcttgcac ctactgattc atcaaaacaa tagttatgga
       61 gtatttatga gtaaggaata tatgaacgat gattcattaa gtgaaaaatg gaaataccga
      121 tttaattttt atgatcaaca tggctttcct ggattctggg gagcaacacc agaatataaa
      181 gcagcattta aagcattaaa agtaagacaa cgattaacta ttcaaatgaa tttcattgct
      241 ttcttctgtt cctggatcta tttatttgtt ctgggcttat ggaaaaaagc aattatagtt
      301 ctattacttg gcattctatc tctatttgtt ggtgcattaa ttggcgttaa cattctggga
      361 attgccgtag cagcctatgt tgctgttaac acgaacaaat ggttttatga gaaagaggta
      421 aaagggttaa atacctggag cctttgaata acaggtggct tagccaggct aagccaccgc
      481 cttcttttta caaccagttc ttccgcttaa agtacagata cggtgccagg cccgcgagga
      541 tcataaagat aatcgcgcca gggtagccga aactccactt cagttctggc ataaactcaa
      601 agttcatccc gtag
//
LOCUS       DAJTRQ010000339          609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000339 DAJTRQ010000000
VERSION     DAJTRQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.345"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>609)
                     /gene="ipaH3"
                     /locus_tag="OO332_004297"
     CDS             complement(<1..>609)
                     /gene="ipaH3"
                     /locus_tag="OO332_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5944490.1"
                     /translation="AVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNAL
                     ISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNN
                     QLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEET
                     EIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          162 a          102 c          167 g          178 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgct
//
LOCUS       DAJTRQ010000340          608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000340 DAJTRQ010000000
VERSION     DAJTRQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.346"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>608
                     /locus_tag="OO332_004298"
     CDS             <1..>608
                     /locus_tag="OO332_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR5944491.1"
                     /translation="AGTGRRVETHEYPNRDKPYTEDLGKVTFRPSITAYVVGDDCFDQ
                     RDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVSTSKGEGRIVRFDLKFVEAGELSY
                     PTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVADFVQNDVVGNASTMLGYVSDAMK
                     VVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQKMWRTGK"
BASE COUNT          132 a          148 c          186 g          142 t
ORIGIN      
        1 tgcgggaacc ggtcgtcgtg tggaaacgca cgaatacccg aaccgcgaca aaccctatac
       61 cgaagatctg ggaaaagtca ctttccgccc gtccatcacg gcttatgtgg tgggagatga
      121 ctgctttgac cagcgcgatc gcctgattga cgcgctgaat aaacccggtc ccggcacgct
      181 tgtccatccg acatacggtg agctgaaagt ctgtgttgac ggggaggttc gggtcagcac
      241 atcgaagggt gaagggcgta ttgtccgctt tgacctgaag tttgtcgaag cgggagaact
      301 ctcttacccc acatcaggtg cggcgacggc gcagacgctg atgtcatcct gttctgcact
      361 ggatgactgc atcagtgaca gtttcagtgg tttcagtatc gatggcgtgg cagattttgt
      421 gcagaacgac gtcgtcggta atgccagcac aatgcttggg tatgtttctg atgcgatgaa
      481 agtggtggat tctgccgtat cggatgtcgc caggctgttg cagggggata tctcggtact
      541 tctgccgccg ccatcgtcag gcaaaaattt cgttgagcag gtgcagaaaa tgtggcgtac
      601 cgggaaac
//
LOCUS       DAJTRQ010000341          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000341 DAJTRQ010000000
VERSION     DAJTRQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.347"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..261)
                     /locus_tag="OO332_004299"
     CDS             complement(<1..261)
                     /locus_tag="OO332_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5944492.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQEL"
     gene            353..439
                     /locus_tag="OO332_004300"
                     /pseudo
     CDS             353..439
                     /locus_tag="OO332_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          176 a          116 c          146 g          167 t
ORIGIN      
        1 aagttcttgt atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt
       61 gagggctggc tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc
      121 ttctgttgca gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt
      181 ttgctcttct acggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga
      241 aggattgtgg acaggtttca tttattattg atcctcattg tttatatatt aatatattcg
      301 tgcaaatgga gtgaggattc tttaaaaaaa cgcgcctgta aatagttaag aacaggaaga
      361 actgttgggg atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac
      421 tgaactgaaa aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc
      481 acagcatcat ttctctgcgc ttatcgagat actgagcatg gttgtaaatt cagctaaccc
      541 agtgcgttga caaaaaatta gcgcaagagg acaaaaaatc accttgcgct aatgctctgt
      601 atcag
//
LOCUS       DAJTRQ010000342          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000342 DAJTRQ010000000
VERSION     DAJTRQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.348"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..594
                     /locus_tag="OO332_004301"
                     /pseudo
     CDS             1..594
                     /locus_tag="OO332_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          175 a          123 c          150 g          146 t
ORIGIN      
        1 ttaattccca aacctaaagg cgttagtgct gatccagagt tgcgtattaa ggtcgtgaaa
       61 gctgtgatcg accagcacat gtcccttaat caggctgctg ctcactttat gcttgctggt
      121 agtggttctg tagccaggtg gctgaaggtc tatgaagagc gcggagaagc tggtttacgc
      181 gcgctcaaga ttggcaccaa aagaaacatt gcaatatcag ttgatccaga aaaagcggca
      241 tcagcattgg agctgtcaaa agaccgacgc attgaggatc ttgaaaggca agttcgattt
      301 cttgaaacgc ggcttatgta tctaaaaaag ctgaaagcct tagctcatcc cacgaaaaag
      361 tgaaagtact caacgagcta aggcagtttt atcctcttga tgagcttctc agggctgcgg
      421 agataccgcg cagtacgttt tattaccatc taaaggctct cagcaagcct gacaagtatg
      481 cggacgttaa aaagcgtatt ggtgagattt atcatgagaa tagaggccga tacggatacc
      541 gtagggtaac gctgtctctt catcgagaag ggaaacagat taaccataaa gctg
//
LOCUS       DAJTRQ010000343          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000343 DAJTRQ010000000
VERSION     DAJTRQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.349"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            34..274
                     /locus_tag="OO332_004302"
                     /pseudo
     CDS             34..274
                     /locus_tag="OO332_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     misc_feature    229..345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            271..>592
                     /locus_tag="OO332_004303"
     CDS             271..>592
                     /locus_tag="OO332_004303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5944493.1"
                     /translation="MMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSAR
                     AQRDDWLKKEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRG
                     KKVRT"
BASE COUNT          142 a          141 c          184 g          125 t
ORIGIN      
        1 ggttctggaa agtcagggcg aatatgactc acaatgggcg acaatttgtt ccattgctcc
       61 aaagattggc tgtacgccgg agactctgcg tgtctgggtt cgccagcatg agcgggatac
      121 cgggggcggt gatggagggc tcaccaccgc tgaacgtcag cgtctgaaag agctggagcg
      181 tgaaaatcgt gaactgcgcc gcagtaacga tatccttcgc caggcttccg cttattttgc
      241 gaaggcggag ttcgaccgcc tctggaaaaa atgatgccac tgctggataa gctgcgtgag
      301 cagtacgggg tcggaccgct atgcagcgaa ctgcatattg ccccgtcaac gtattaccac
      361 tgtcagcaac agcgacatca tccggataaa cgcagtgccc gtgcgcagcg cgatgactgg
      421 ctgaagaaag agatacagcg cgtatacgat gaaaatcaca aggtatacgg tgtgcgtaaa
      481 gtctggcgtc agttgttacg ggaaggtatc agagtggcca gatgcactgt ggcacgtctc
      541 atggcggtta tgggacttgc cggtgttctc cggggtaaaa aggtccgtac ga
//
LOCUS       DAJTRQ010000344          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000344 DAJTRQ010000000
VERSION     DAJTRQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.350"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..365)
                     /locus_tag="OO332_004304"
     CDS             complement(<1..365)
                     /locus_tag="OO332_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5944494.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSF"
BASE COUNT          149 a          160 c          135 g          148 t
ORIGIN      
        1 tcaaacgact ttagcaatgc cgtatcccgg gttctgcgtg tcgcaacggt attcagatat
       61 tgctctgcgg cttcgctcag agctggcgta tgttcccgac ctactgattc gcacaggtac
      121 tcaacgaagc ctgataccag ttctcgttct cgaggcgtga tcgccccacc gaccggagtc
      181 cggtaatcga atcccagttg caggagtttg aaaaaacgct tgtggaatgc gtagttacga
      241 acgcgcttaa aatcagcgtg tatccactcg cctgttttga tttgatgcag aaattcgcaa
      301 ctctccgacg tcgccgggag aagtaatccg gaagaggttt gtttgaccag ttgtatatgt
      361 gccatcggct ttctccggtg gcacggtgtt actcagcagg gggtcaaccc tgcactgaat
      421 tgtagatgaa ctcactcaca ttcaaaagca gaaaagccag ccttaagtcc agtctctttc
      481 agcgcccgta atgacgtgac aaattcatct ccacacaaaa gaaaaccatc cgtcacaagc
      541 ccatccacca aatgaattaa cgcagctcca ctcttccttt gttgagactg ta
//
LOCUS       DAJTRQ010000345          589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000345 DAJTRQ010000000
VERSION     DAJTRQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.351"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            193..462
                     /locus_tag="OO332_004305"
     CDS             193..462
                     /locus_tag="OO332_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944495.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..589
                     /locus_tag="OO332_004306"
                     /pseudo
     CDS             459..589
                     /locus_tag="OO332_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          145 a          138 c          170 g          136 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggca
//
LOCUS       DAJTRQ010000346          588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000346 DAJTRQ010000000
VERSION     DAJTRQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.352"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            158..>588
                     /locus_tag="OO332_004307"
     CDS             158..>588
                     /locus_tag="OO332_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5944496.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLA"
BASE COUNT          189 a          127 c          132 g          140 t
ORIGIN      
        1 aaaaaaagcc gaatttctgg caactctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
       61 aaaagaaagc ctgcacactg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      121 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg aacaccgata aacaagttta
      181 cccactgtat tacgaagcaa aaaatgacaa agtaagaaaa cgtctcggta ttaaaggcgg
      241 tttttactgg gctgaagcga aaaaattatc cattgccatc tcccgtggtg ctgttgcgat
      301 tgacgatgca ggctacgatg aagatgactt taaaaaacct gttcgcgtca atttgcccgt
      361 tgttgatgac ctcccgccag aaggcgtatt tgatacggaa ttctgcaacc gttacgaaaa
      421 aggcggggaa gatggcatca caatggtatt tatcgcatct tctccctctg ttcaggacaa
      481 accggccagc actgacaata ccaacgtcaa cggcgaagac atgacagaaa ttgaggagaa
      541 catgctcctg ccagtttccg gtcaggaact gcccattcgc tggcttgc
//
LOCUS       DAJTRQ010000347          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000347 DAJTRQ010000000
VERSION     DAJTRQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.353"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..528)
                     /locus_tag="OO332_004308"
     CDS             complement(<1..528)
                     /locus_tag="OO332_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="catalyzes the transfer of
                     2-keto-3-deoxy-D-manno-octulosonic acid to lipid A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HCR5944497.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRR"
BASE COUNT          145 a          143 c          172 g          127 t
ORIGIN      
        1 acgacgcagc aagcggcgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc
       61 agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag
      121 gttaggccat agttcggttt ccataatcaa caccagtttg gggtcgactt tattcaggaa
      181 acggttgagc gcatcgggca ggtcatacgg cagataaacg tgctgaacat ccttcccgaa
      241 agccgattgt acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa tcggtaaatc
      301 aggataacga tgacgcagcg cgcgcaccaa cgggatcgcc gccagagttt caccgacgga
      361 gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc
      421 accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat
      481 cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg
      541 acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttacc
//
LOCUS       DAJTRQ010000348          582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000348 DAJTRQ010000000
VERSION     DAJTRQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.354"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..385)
                     /locus_tag="OO332_004309"
     CDS             complement(<1..385)
                     /locus_tag="OO332_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5944498.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENL"
BASE COUNT          126 a          162 c          128 g          166 t
ORIGIN      
        1 gcaagttttc aaccagtgcg ctcaccgcgc gaccccagga aaaaccgaca atatcgccag
       61 gctccagcaa gcgatccagc agttgcgcgc cgtgtaaccc catcatcgcc agttgagtct
      121 cttcatcctc atcatttccc gacaccacca caacgtcttt caggccaaac ttttgcttca
      181 gttgctgctc cagccagaga ttttcgttgt agtcatagtt gatggcgatg gtgaccattc
      241 cctgatcgcg gcctcgttta agcaagcggc tgatggtggt gcggtaaata cccagttcgc
      301 gcgcgatttg cgcctgcgtc atatcctgtt cgtaatagag ttgggcaatc ttcacaatca
      361 aacggatatc gtcactgttt tccattgccg cctcctggtt ctgcacattt gtgcaaatac
      421 gctttctcac cgcttgatct gatataaccc catcatgagg gcctttcaaa gcgatttttc
      481 actctttgca tggggagaaa tagcgctttc tcttgcacat ttgtttctct gcgtagtagt
      541 catcagcaca ggtgtgcagc gattcgtaat atgttgatta tc
//
LOCUS       DAJTRQ010000349          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000349 DAJTRQ010000000
VERSION     DAJTRQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.355"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..178
                     /locus_tag="OO332_004310"
     CDS             <1..178
                     /locus_tag="OO332_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5944499.1"
                     /translation="TNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPR
                     IARVRSVEEILHAL"
BASE COUNT          178 a          139 c          116 g          148 t
ORIGIN      
        1 gaccaatatc ggtgttgaat ccaccgcccg caatgcctgg gaacttggct ttaatctggt
       61 gattgccgaa gacgcctgta gtgccgccag cgccgagcaa cacaataaca gcattaatca
      121 tatctacccg cgcatcgccc gtgtgcgtag cgtggaagag atccttcacg cgttatgatt
      181 tacatcggtc taccgcaatg gtcgcatcct aaatgggtgc ggttggggat caccagcctt
      241 gaagagtatg cccgccactt taactgcgtg atacgggatt tttaaaaata actaaagaac
      301 gcccaagagc atgtgttttc tttagaataa tcaaagcgtt gtattgccat gtcatatgaa
      361 aatcggcaaa gttcatatgt aacaaagtgt cccaatcatg ccccaaaatg ccccacaaca
      421 aatatttttg ccccatgcat gccccaaaaa atcactttcc ctcactcttg acactgttta
      481 tccatacagt taaaaataat actgtataaa aacacagtgt agagggattt ttatgcgtat
      541 tgaaatctgc atagccaaag agaaaatgac taaaatgcca a
//
LOCUS       DAJTRQ010000350          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000350 DAJTRQ010000000
VERSION     DAJTRQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.356"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>577
                     /locus_tag="OO332_004311"
     CDS             <1..>577
                     /locus_tag="OO332_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCR5944500.1"
                     /translation="MLLVTPLVIYTMYPPEIKKVDNKTISKAGLAELGPMKIREKMLL
                     GVFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGI
                     IGLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAM
                     LPVFAMLANVSGAPLMLTALALLFSNSYGGMV"
BASE COUNT          130 a          127 c          141 g          179 t
ORIGIN      
        1 tatgctgctg gtcaccccgc tggtaattta caccatgtat ccaccagaaa ttaaaaaggt
       61 ggataacaaa accatctcta aagcgggcct tgccgaactg ggaccgatga aaatccgcga
      121 aaaaatgctg ctcggtgtct ttgtgctggc gctactgggc tggattttca gtaagtctct
      181 gggggttgat gaatccactg tggcaatcgt tgttatggcg accatgctgc tgctgggtat
      241 cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa tctggtacgg
      301 cggtattatc ggcttaagct ccttattatc gaaagttaaa tttttcgaat ggttagctga
      361 agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct tcgttattat
      421 tttcctcagc atcatcgtgc gttatttctt cgcttccggt agtgcctata tcgttgctat
      481 gttacctgta tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct
      541 ggcactgttg ttctccaact cctatggcgg catggtt
//
LOCUS       DAJTRQ010000351          567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000351 DAJTRQ010000000
VERSION     DAJTRQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.357"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            1..147
                     /locus_tag="OO332_004312"
                     /pseudo
     CDS             1..147
                     /locus_tag="OO332_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            144..>567
                     /locus_tag="OO332_004313"
     CDS             144..>567
                     /locus_tag="OO332_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR5944501.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDH"
BASE COUNT          127 a          138 c          166 g          136 t
ORIGIN      
        1 gactagtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtgata
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcga
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaagc gctctgggat catctgggga
      361 ataatcaggt gctgtcggta aaccgattgg tttacttttc attgctgaaa aaattgttcc
      421 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acgcttcttc
      481 gccatatatc gatactcaac gggtttgaca atctgatggc tcagccgttg ttgtcagatg
      541 agcccctgac agcgctgttg gatcatt
//
LOCUS       DAJTRQ010000352          561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000352 DAJTRQ010000000
VERSION     DAJTRQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.358"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..417
                     /locus_tag="OO332_004314"
     CDS             <1..417
                     /locus_tag="OO332_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5944502.1"
                     /translation="AWTYRHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAP
                     VAGSDLYVFRPVAHTVDFHIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISE
                     AISGADGEYSHQLLAPVDNISIAKNELAVLGTISWT"
     gene            408..>561
                     /locus_tag="OO332_004315"
     CDS             408..>561
                     /locus_tag="OO332_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944503.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELD"
BASE COUNT          119 a          143 c          175 g          124 t
ORIGIN      
        1 gcatggacat accgtcactg gatgggaacg ggaactgttg gtgtgatgat tgccagcagt
       61 gacctgatta accccattcc ggaagaatca acggaaacgg cggcaagaca acatatcggg
      121 ccactggccc cggtggcagg ctctgatttg tatgtgttca ggccggtggc acatacggtg
      181 gattttcata tccgcgtgac gccggacaca ccggaaatac gggctgccat taccgcggag
      241 ttgcgttcgt tcctgctgcg tgatggttat ccgcagggag aactgaaggt atcgcgtatc
      301 agtgaggcga tttccggtgc ggacggggaa tacagccatc agttgcttgc accggtggac
      361 aatatctcca ttgcgaaaaa cgaactggcg gtactgggga cgatttcatg gacgtgacaa
      421 acgatgatta catccgcctg ttatcggcac tgttgccgcc cggtccggcg tggtcatcta
      481 gcgatccggc gattgccggt gcggcaccgt cattaacccg tgttcatcag cgtgcggatg
      541 ccctgatgcg ggagctggat c
//
LOCUS       DAJTRQ010000353          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000353 DAJTRQ010000000
VERSION     DAJTRQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.359"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..122
                     /gene="kil"
                     /locus_tag="OO332_004316"
     CDS             <1..122
                     /gene="kil"
                     /locus_tag="OO332_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5944504.1"
                     /translation="NIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            122..292
                     /locus_tag="OO332_004317"
     CDS             122..292
                     /locus_tag="OO332_004317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944505.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            367..>557
                     /locus_tag="OO332_004318"
     CDS             367..>557
                     /locus_tag="OO332_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944506.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLV"
BASE COUNT          205 a           97 c          110 g          145 t
ORIGIN      
        1 acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca tgcatttctg
       61 actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt
      121 aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg aagaaagcaa
      181 aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg aaatgaaaaa
      241 atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt aattaactga
      301 ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt aattaaggag
      361 aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat tgttcacgag
      421 gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa agaaacccca
      481 ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa cgatggtgta
      541 aaaaaaatgt gtctggt
//
LOCUS       DAJTRQ010000354          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000354 DAJTRQ010000000
VERSION     DAJTRQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.360"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          174 a          120 c          116 g          145 t
ORIGIN      
        1 gtgccagttg agcaaaggtt cggagctgat tattcacttc tgattttgcc caaaggaagg
       61 caatccatag cgaaagaatg atgggaaaga gaactaaaaa gataatgccc ggcaacgcca
      121 gtaattggtg tcgtgcacga tgactcattg ctatcaattt cctgcctgtt tatacccggc
      181 cattgcaagg tgaaaccctg gctaccgacg tctttagata aatcttacgg tataaatagc
      241 acttttgcct gatttgaaat tgataccttt ttaatcgtca cctggatgaa aataattcat
      301 tgatattcaa aattaatttt ctacaggaat acagaggcgg cgggaagata tgcccttaat
      361 gccactgaaa aaccacataa tctgtacagc aataaaagtc acggcctaat atggctaaca
      421 aacaatcaat tgtgatatgg ttcacaaaat taatgaaaca aacagagtgt ttcatttttg
      481 tgttctgcaa gccccgcgat agccagagtg taccacgcct cccgtgaaca acgccgcgct
      541 gtccagggca tcggc
//
LOCUS       DAJTRQ010000355          540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000355 DAJTRQ010000000
VERSION     DAJTRQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.361"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            12..>540
                     /gene="fimC"
                     /locus_tag="OO332_004319"
     CDS             12..>540
                     /gene="fimC"
                     /locus_tag="OO332_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5944507.1"
                     /translation="MTFCLLAGILMFMAMMVAGRAEAGVALGATRVIYPAGQKQVQLA
                     VTNNDENSTYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDR
                     ESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSA
                     NSLTLINPTPYYLTVT"
BASE COUNT          165 a          115 c          127 g          133 t
ORIGIN      
        1 aatcgcagga aataacattc tgcttgctgg caggtatcct gatgttcatg gcaatgatgg
       61 ttgccggacg cgctgaagca ggagtggcct taggtgcgac tcgcgtaatt tatccggcag
      121 ggcaaaaaca agtgcaactt gccgtgacaa ataatgatga aaatagtacc tatttaattc
      181 aatcatggga ggaaaatgcc gatggtgtaa aggatggtcg ttttatcgtg acgcctcctc
      241 tgtttgcgat gaagggaaaa aaagagaata ccttacgtat tcttgatgca acaaataacc
      301 aattgccaca ggaccgggaa agtttattct ggatgaacgt taaagcgatt ccgtcaatgg
      361 ataaatcaaa attgactgag aatacgctac agctcgcaat tatcagccgc attaaactgt
      421 actatcgccc ggctaaatta gcgttgccac ccgatcaggc cgcagaaaaa ttaagatttc
      481 gtcgtagcgc gaattctctg acgctgatta acccgacacc ctattacctg acggtaacag
//
LOCUS       DAJTRQ010000356          537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000356 DAJTRQ010000000
VERSION     DAJTRQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.362"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(100..279)
                     /locus_tag="OO332_004320"
     CDS             complement(100..279)
                     /locus_tag="OO332_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944508.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
BASE COUNT          109 a          114 c          135 g          179 t
ORIGIN      
        1 gcgtgcctgt aacagttgct ccagcctttt tattttgttc tcattcatcc ggttttcctc
       61 gtatgctgca tcacaccact gtgctaatga aaaatgtaat cagtgtggtg gacgtgtttt
      121 atccgcattg gaattgtcgt caactaaagg agatgaggcc cgtttgtatc gcaatgggtc
      181 tatggcgctt atgcccgttt gctgttcgat aatggcttct ctgagcatac ggtgttgcag
      241 ttctcgtaaa aactttgcgt cttctggtga taaatccatt gttattactc gtaaagcaat
      301 gcgctgtagt gccttacgct accgcgctga tggttcttat ccgttttatt gaatcggaga
      361 acaaactgtg agctatcagc ctggtggtct ggttttgcct gatttaagcc gccgtgggta
      421 tttgtccaag aatgcttcag gctccaatcg ttttggcctg gtgaatggag atgggttaag
      481 caaagactct cgttgctggt gtactttttc gcgctggcgc tcaagcatgg ataatgc
//
LOCUS       DAJTRQ010000357          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000357 DAJTRQ010000000
VERSION     DAJTRQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.363"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>533
                     /gene="pyk"
                     /locus_tag="OO332_004321"
     CDS             <1..>533
                     /gene="pyk"
                     /locus_tag="OO332_004321"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5944509.1"
                     /translation="PALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHG
                     GENIHIISKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIR
                     ARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSI
                     MATICERTDRVMNSRLE"
BASE COUNT          147 a          141 c          134 g          111 t
ORIGIN      
        1 gccagcactg gctgaaaaag acaaacagga cctgatcttt ggttgcgaaa aaggcgtaga
       61 ctttgttgct gcttccttta ttcgtaagcg ttctgacgtt atcgaaatcc gtgagcacct
      121 gaaagcgcac ggcggcgaaa acatccacat catctccaaa atcgaaaacc aggaaggcct
      181 caacaacttc gacgaaatcc tcgaagcctc tgacggcatc atggttgcgc gtggcgacct
      241 gggtgtagaa atcccggtag aagaagttat cttcgcccag aagatgatga tcgaaaaatg
      301 tatccgtgca cgtaaagtcg ttatcactgc gacccagatg ctggattcca tgatcaaaaa
      361 cccacgcccg actcgcgcag aagccggtga cgttgcaaac gccatcctcg acggtactga
      421 cgcagtgatg ctgtctggtg aatccgcaaa aggtaaatac ccgctggaag cggtttctat
      481 catggcgacc atctgcgaac gtaccgaccg cgtgatgaac agccgtctcg agt
//
LOCUS       DAJTRQ010000358          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000358 DAJTRQ010000000
VERSION     DAJTRQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.364"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..464
                     /locus_tag="OO332_004322"
     CDS             <1..464
                     /locus_tag="OO332_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5944510.1"
                     /translation="IPLVSQVAFSTSSWRRAVLSLEEHHKAWLLWCYSGSICWEYQIT
                     ITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAELFGGEGYEYQELALLAGV
                     TTKNWSKTFTAHWVVMKHIFHRLDSEALLFVMRTRSEQKAAFSKQSIAKVD"
BASE COUNT          159 a           97 c          137 g          139 t
ORIGIN      
        1 caattcctct ggtcagtcag gttgcgtttt cgacatcatc gtggcggcgg gctgttctgt
       61 ctcttgaaga gcaccataaa gcctggttgt tgtggtgtta cagcggaagt atttgctggg
      121 agtatcagat caccataacc cactgggcgt gggaagagtt taaggctcat tctggcacta
      181 ggaaaattgc agagaaaaca caggaacgcc tgaaaaaatt aatctggctg gcggcgcagg
      241 cggtaaaagc agaacttttt ggtggggaag gttatgaata tcaggagctg gcattactgg
      301 cgggagtaac aaccaaaaac tggtccaaaa catttactgc tcactgggtt gtaatgaaac
      361 acatttttca ccgactggat agtgaggctt tattgtttgt gatgagaaca cgttcagaac
      421 aaaaagcggc attttcaaag caaagtattg caaaagtaga ttaaaaggca tatatttcat
      481 gcaaatctga tactttgccg attttgtacg cgatggtaaa gtaagcaaaa cc
//
LOCUS       DAJTRQ010000359          529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000359 DAJTRQ010000000
VERSION     DAJTRQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.365"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            259..>529
                     /locus_tag="OO332_004323"
     CDS             259..>529
                     /locus_tag="OO332_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5944511.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALE"
BASE COUNT          193 a           91 c           79 g          166 t
ORIGIN      
        1 ggtataaccc gctttattcg ccacatcatc aagcagtaaa gactgatcta aattatgttc
       61 aatccataat agaacatctt tgataaccgt tgtatgaaac tgcttatcat catatcttaa
      121 ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca taaaataacc
      181 taaatgttct taagattgtc acgaccacat catcatgata ccataaacat actgacggta
      241 tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc
      301 tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt
      361 tcaatagatg agatctcggg aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt
      421 aaaaataaaa aacaattact cagtgcctgt tataagcagc aattaattat gattgatgcc
      481 tacatcacaa caaaaactga tttaacaaat ggttggtctg ccttagaaa
//
LOCUS       DAJTRQ010000360          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000360 DAJTRQ010000000
VERSION     DAJTRQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.366"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..356)
                     /locus_tag="OO332_004324"
                     /pseudo
     CDS             complement(1..356)
                     /locus_tag="OO332_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          146 a          119 c          132 g          128 t
ORIGIN      
        1 ttgcgccttg ctgccaacga agcgccactg ctcatcaagt tcacagataa gggcaacatc
       61 agcgtgagcg actggcgcag atgttatccg ccggggcgag agctttttaa tgtccgtatg
      121 acggtattga tgccgatttt cagtgttctg gcggtataac agccccctgc gccgttatgt
      181 gccatttcaa cgatctgctc cttaacgccg agtattctgg cttcataaga gtaagtgagc
      241 tggaaaacac gtttacatga gcgacaacgg aaacgctcat gccgggaaca actacggcca
      301 tgtcgataaa cttcatcgga attacagcgc ggacaatgaa cagtaacggt agtcatggaa
      361 aacctcaaat accagtagat taccagaaac ttctatcgat tgaagacatt accttttgcc
      421 gcaataggtg gggtaacatt ggtgaaaacg cattacgccg ggtggcagtt ggaaaaaaat
      481 tatctttttt cggctctgat cgtggtggag aaaatgcggc aataa
//
LOCUS       DAJTRQ010000361          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000361 DAJTRQ010000000
VERSION     DAJTRQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.367"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..522)
                     /locus_tag="OO332_004325"
                     /pseudo
     CDS             complement(1..522)
                     /locus_tag="OO332_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
BASE COUNT          151 a          133 c          137 g          101 t
ORIGIN      
        1 gacagcacgg tgatgcagac ggttgcccat accagcgagg tataaccaaa cagacgttta
       61 cgcgagaagg ttgccgcaat ttcggagaac acaccgaaaa caggcaggat caggatgtaa
      121 acttccgggt ggccccaggc ccaaatcagg ttgatgtaca tcatcatgtt gccacccata
      181 tcgttggtaa agaaatgggt gcccagatag cgatccaggg tcaacaacgc gacggtaacc
      241 gtcagaattg ggaaggaagc aataatcagt acgttcgtgc acagtgatgc ctaggtaaat
      301 actggcatct tgaacatggt catgcccggt gcgcgcatct tcagaatggt aacgaagaag
      361 ttgataccgg taagcgtcgt acctataccg gatagctgga gactccatat ccagtaatca
      421 acaccgactc ccggactgta ctctattccc gatagcggtg gataggccag ccagccggtc
      481 tgcgcaaatt cgcccacgcc gagagaaacg ttaaccagaa tc
//
LOCUS       DAJTRQ010000362          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000362 DAJTRQ010000000
VERSION     DAJTRQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.368"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..502
                     /locus_tag="OO332_004326"
     CDS             <1..502
                     /locus_tag="OO332_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944512.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DAJTRQ010000363          512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000363 DAJTRQ010000000
VERSION     DAJTRQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.369"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          118 a          126 c          142 g          126 t
ORIGIN      
        1 tacccgatta gttgttaatc atatttaatc atagtgatta ctttcagacg gaacaaggca
       61 aataatgcga gtcagcgcat ataaggcggc atacggtggt tacatgtagc acaggtgtaa
      121 aaccgccgaa gcccggcgca agccggtacg gtcgtccgaa tatcaccagt ccccctccgg
      181 ctctgcgagg ccatgcggcc tgtcagcgat gaaaatgcct ctgtgcgcgg tcggagtggt
      241 cccgacaagg gtttacccgc agtcggggcg tatctccgcg ttagcgggcc gtgagggccg
      301 cttacgagcg tgtatgagag acgtacagta gttttgtccg ctcacaccag tacaaaccag
      361 tcgaaatacc ggtaacgacg attactcttg tctctgatgc cggtggacta cgagatttta
      421 tttactggcg tcctgatgcg gtcaggactg gtatggaacc tgtttatgtg atacttaatg
      481 ctcttctgga ttccgggcga tttacgcgta aa
//
LOCUS       DAJTRQ010000364          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000364 DAJTRQ010000000
VERSION     DAJTRQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.370"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(9..>510)
                     /gene="ospZ"
                     /locus_tag="OO332_004327"
     CDS             complement(9..>510)
                     /gene="ospZ"
                     /locus_tag="OO332_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5944513.1"
                     /translation="EKSRRLNYMKTMYSPCPEDFQPISRDEASTPEGSWLTVISGKRP
                     MGQFSVDSLYHPDLHALCELPEISCKIFSKENSDFLYIIVVFRNDSPQGELRANRFIE
                     LYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMNDRLSQIK
                     ARMPVT"
BASE COUNT          156 a          106 c           78 g          170 t
ORIGIN      
        1 aatatacatt aagtaacagg cattcgagcc ttgatttgag aaagcctgtc attcatttgt
       61 tttatgtagc tatctatttc ctctgatgca taactaaaaa gttctcccat ttcacgagca
      121 ataataatct cagatttaat agactttaac tctggcgact catctcttaa cacttgcata
      181 atctctcttt ttatatcata tagttctata aaccgatttg cccgtagttc tccttgggga
      241 gaatcatttc tgaatacaac aattatatac aaaaaatcac tattttcttt agagaagatc
      301 ttacaactaa tctctgggag ctcacataat gcatgtaagt caggatggta caaactatcc
      361 acagaaaatt gccccatagg acgttttcca gatataactg tcaaccagct tccttccggt
      421 gtactggctt cgtctctgga aataggctga aaatcttccg gacatggaga atacattgtc
      481 ttcatataat taagtcgtct ggatttctca
//
LOCUS       DAJTRQ010000365          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000365 DAJTRQ010000000
VERSION     DAJTRQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.371"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>508
                     /locus_tag="OO332_004328"
     CDS             <1..>508
                     /locus_tag="OO332_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR5944514.1"
                     /translation="HQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYN
                     CFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGH
                     EIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLN
                     ASDKLFYLP"
BASE COUNT          158 a           91 c          103 g          156 t
ORIGIN      
        1 atcatcagga acaagtaaat ggaccgcttg tcgacgagtc agggcgggtt atttttgccg
       61 attttaaccc atcgaaacga ccgtggtcgg tggcttcaga tgactctaac aacagctgga
      121 atccggcata caattgcttt gatcgtccgg gtaaaaaatg tatctctttt acgctacgta
      181 tcaacggcaa agatcacgat ttgttagcgg tagataaaat tcatgtcgat ttaaactggc
      241 ggtatctgaa cgagtatctt gatcaaatca gcgctaatga tgaagttcta tttttgaaac
      301 aaggtcatga gatcattgcc aagaatcaac tcgctcgtga aaaactgatt atttataata
      361 gagaaggtaa ttataatatt attgattctg tcgatacgga gtatattgca aagacatcag
      421 cagtgccaaa taatgcattg ttcgaaatct atttttatta tcctggcggt aatttattga
      481 acgcatcaga taaacttttt tatctacc
//
LOCUS       DAJTRQ010000366          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000366 DAJTRQ010000000
VERSION     DAJTRQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.372"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          170 a           96 c          112 g          128 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catatcaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggg
//
LOCUS       DAJTRQ010000367          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000367 DAJTRQ010000000
VERSION     DAJTRQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.373"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..269
                     /locus_tag="OO332_004329"
     CDS             <1..269
                     /locus_tag="OO332_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5944515.1"
                     /translation="YCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAAIIY
                     SLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          122 a          112 c          136 g          134 t
ORIGIN      
        1 tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac gcgctgcgat
       61 cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt ggagaaagcg
      121 cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag ccggaggact
      181 ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg catgaactgc
      241 tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc tgggcgctta
      301 ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc atgtgtttta
      361 tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat gatcggcatt
      421 gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca caatccgggc
      481 tgagttccct ctaagtgatc tagg
//
LOCUS       DAJTRQ010000368          504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000368 DAJTRQ010000000
VERSION     DAJTRQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.374"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            3..494
                     /gene="mug"
                     /locus_tag="OO332_004330"
     CDS             3..494
                     /gene="mug"
                     /locus_tag="OO332_004330"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR5944516.1"
                     /translation="MAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQAGFTDR
                     QLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQALAIL
                     GKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELDQALVV
                     RGR"
BASE COUNT          118 a          119 c          153 g          114 t
ORIGIN      
        1 ttttggctcc agggttacgg gtcgtgtttt gcggtatcaa ccctgggctt tcatccgccg
       61 ggactggttt tccctttgct catccggcaa atcgcttctg gaaggtgata tatcaggccg
      121 ggtttaccga ccgtcagttg aagccgcagg aggcacagca tctgctggat tatcgttgtg
      181 gcgtcaccaa actggtagac cgtccaacgg tgcaagccaa tgaagtttca aagcaggagc
      241 tgcacgcagg tgggcgtaag ctgattgaaa aaattgaaga ttatcagccg caggcgttgg
      301 cgattctggg caaacaagca tatgaacagg gattcagcca gcgcggtgca cagtggggga
      361 aacaaacgct caccattggt tcgacgcaga tttgggtgct gccaaatccc agcggtttaa
      421 gtcgcgtttc actggagaaa ctggttgaag cgtatcgcga gctggaccag gcgctggtag
      481 tgcgtgggcg ataaaaaacg ccac
//
LOCUS       DAJTRQ010000369          502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000369 DAJTRQ010000000
VERSION     DAJTRQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.375"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>502)
                     /locus_tag="OO332_004331"
     CDS             complement(<1..>502)
                     /locus_tag="OO332_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5944517.1"
                     /translation="SGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHT
                     GTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTLQRL
                     NDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQP
                     GELVNV"
BASE COUNT          118 a          126 c          129 g          129 t
ORIGIN      
        1 tcgacattga ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca
       61 agctgaggaa cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta
      121 tacagcaccg aaaaagtgat gttaggcgca aaggaattgc tcactggaac ccgggcttca
      181 tacagggtat cgtttaaacg ttgtagcgtt agccagtttg ccggatgcga aagcagcgac
      241 tgctgttcca cgcgatcgcg ttccagcgtc aataacgctt catcaattgg ctccgggaaa
      301 gtaatcagca tcttcgcggt ttcgcctggc tggtacagcg ttttatccgc cacgatatct
      361 accgtaccag tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga
      421 attaagccgt ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg
      481 gtaaaggatt tgccgcctga cg
//
LOCUS       DAJTRQ010000370          496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000370 DAJTRQ010000000
VERSION     DAJTRQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.376"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(301..>496)
                     /locus_tag="OO332_004332"
     CDS             complement(301..>496)
                     /locus_tag="OO332_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5944518.1"
                     /translation="GGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDKSDY
                     VLAHGLNTDDQLAVGIVYQF"
BASE COUNT          133 a           84 c          130 g          149 t
ORIGIN      
        1 tcccggattg gtagggaaaa gcttttttca tgcactattt ttgcaaatat ttctatatgg
       61 gaagcggttt tgttacagat taaatttata aagaaatgca tttggggtgt tgggttgaag
      121 agtagctggt agatgttatt gtgccagcag gggcagggga atgtgccgta tcgatatgct
      181 ttggcaattg cgtgtgccgc agatggagat gaaatgccaa taaaaaagcc tgagttcgat
      241 gagggacgaa ctcaggctgg tgttatgttt tacagtaaca cgcctcggcg cgttggctga
      301 ttagaactgg tagacgatac caactgccag ttggtcgtcg gtgttcagtc catgagcgag
      361 aacataatca ctcttatcaa gcaggttgat ctgataagcg gtatatatgt tcatattctt
      421 attgaagtaa taccaggtac cgatttcaat atagttcaga cgatcagaat ctttgtaacc
      481 tgtagactgg ccccct
//
LOCUS       DAJTRQ010000371          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000371 DAJTRQ010000000
VERSION     DAJTRQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.377"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            71..495
                     /locus_tag="OO332_004333"
                     /pseudo
     CDS             71..495
                     /locus_tag="OO332_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          132 a          116 c          136 g          111 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacga
//
LOCUS       DAJTRQ010000372          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000372 DAJTRQ010000000
VERSION     DAJTRQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.378"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..494)
                     /locus_tag="OO332_004334"
                     /pseudo
     CDS             complement(1..494)
                     /locus_tag="OO332_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          132 a          127 c          118 g          117 t
ORIGIN      
        1 ctggcgttcg acgatattgg tccacaaatt gtctttatcg tcagtccaca aattaatcag
       61 caaggcaaaa aagcgttcag ctgccggaga aagcacggca tctttgcggc gaataatccc
      121 caatgtccga cgtataacgg gttccaccag tgggatacca ataatcgaag aatagggcgc
      181 atggggcatt gccagaccag gaagcgccga aatccccagt cctgcctcca ctagtcctaa
      241 tgacgtcgaa agatgacgca cttcgtaaaa ccagtccagc ttccagggct tgtcggctag
      301 ctgttgttct atcaacaatc ggttgccgct ggaggaacgt acgccaatca ttttgtaacc
      361 cactaactcc tgccattcta caagctactt tttggccaat ggatggtcac gccgacaggc
      421 cagcacgaat ggctcgttga ccagtggggt aaaatcaatg gatgaatttg tgacgttgtt
      481 catgtttatg ccaa
//
LOCUS       DAJTRQ010000373          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000373 DAJTRQ010000000
VERSION     DAJTRQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.379"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(16..>493)
                     /locus_tag="OO332_004335"
     CDS             complement(16..>493)
                     /locus_tag="OO332_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5944519.1"
                     /translation="VIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLS
                     TGLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQ
                     GLIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
BASE COUNT          136 a          147 c          112 g           98 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata acc
//
LOCUS       DAJTRQ010000374          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000374 DAJTRQ010000000
VERSION     DAJTRQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.380"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..76
                     /locus_tag="OO332_004336"
     CDS             <1..76
                     /locus_tag="OO332_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5944520.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            110..>490
                     /locus_tag="OO332_004337"
     CDS             110..>490
                     /locus_tag="OO332_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944521.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEADRAK
                     QVALIAAAQDAETKAVELTVRAKAE"
BASE COUNT          154 a          115 c          133 g           88 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gccgatcgtg ctaaacaagt
      421 tgccctaatc gctgccgcgc aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc
      481 gaaagcagaa
//
LOCUS       DAJTRQ010000375          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000375 DAJTRQ010000000
VERSION     DAJTRQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.381"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            96..486
                     /locus_tag="OO332_004338"
                     /pseudo
     CDS             96..486
                     /locus_tag="OO332_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT          114 a          124 c          135 g          113 t
ORIGIN      
        1 gcgcatgaag acggaactag tccagatgtg atatttgtgg tgatcacata catcatcaac
       61 gatgctcgtt atggtgagtt tgatgactac ccgctgaagt gaaaatagtg ttgtgtacca
      121 aattgcgtac taaactaaaa tcacaaatca tgaaaccctt gttcatggcg gttctcaggg
      181 gtattgcgcg taatcgcgaa ttcagggtat ggacccggtg tgggtcactg ctgcgatccc
      241 ggagtccatc gcctggctgg tgaaagatgc ctcgcagttt accctcaccg ttccggcgcg
      301 accggataaa acactcacca tccgcaaatg gacgctgcta cctggcgtgg atgccgcgac
      361 ccgcacgctg cagctgcgtc tggaagtcga caacgccgac gaggcgctga ttgataccgg
      421 caacgaacag cgggtgatta ccgttgatgc cgacgggcgc tttgtaccga aacgcgttgc
      481 tgtttt
//
LOCUS       DAJTRQ010000376          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000376 DAJTRQ010000000
VERSION     DAJTRQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.382"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..347)
                     /gene="ycaL"
                     /locus_tag="OO332_004339"
     CDS             complement(<1..347)
                     /gene="ycaL"
                     /locus_tag="OO332_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5944522.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRV"
BASE COUNT          130 a          114 c           98 g          144 t
ORIGIN      
        1 tagacacgaa cacagccgtt cgccatcgcc catgcgttga cgtcgctggt catataaacc
       61 ttatagttga ccggcgtgcc attaatattg ttacccaatg ctttggcgat ttttgccaga
      121 cgtttaccgt acttgctgga tttacttgcg acttgattgc cgctgtccat ttcggcacag
      181 ccctgattgg caatcgcttt tgcatcggca tcgcttaatg ttgctgcttt ataggcattt
      241 aaaccggagc tgatagccat attggtatca ataccgtggg tattttgaca ccctgtcagt
      301 aatgctgcag aggtcgcaat cgccagcagt aatttagtat tcttcatgtg aatcatccgt
      361 tgattaatca tatataaata ttatgtatca acaacttaca ttgacaagcg acgctaccat
      421 taatcaaaat gactcacaag gtccgaaaaa aaacgccgac aatgtgccgt aatgccagtc
      481 agttaa
//
LOCUS       DAJTRQ010000377          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000377 DAJTRQ010000000
VERSION     DAJTRQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.383"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(172..402)
                     /locus_tag="OO332_004340"
                     /pseudo
     CDS             complement(172..402)
                     /locus_tag="OO332_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT          153 a          120 c           93 g          120 t
ORIGIN      
        1 ttatgcaata tattgaattt gcatggtctt gtaggccaga taagacgttc acgtcgcatc
       61 cggcatgaac aaagcgcact ttgtcaaaaa tctaacctac tttttaattc agggaattac
      121 cgcaaagccc acgccatcat acccaacgta acaaaactca ggcacgttcc ccactcgccc
      181 cgagaaaaat agcattaatg cgcccagcgt cagcataaaa atattgagca gaaggtaaat
      241 tgttggcgta aagagtaaat tgcaaacctc aggaatagcg gcaagcccag cccccagcat
      301 ggcaataacc gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag tcattaccag
      361 cgaggtcaaa atccattttc tattttgcat tattctttcc attctttctg aatggcgaaa
      421 ttatactccc cttgcccctt ctggacactt ttccgaaatg atggcgaaaa aaacgggacc
      481 ctttgg
//
LOCUS       DAJTRQ010000378          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000378 DAJTRQ010000000
VERSION     DAJTRQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.384"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(109..>484)
                     /locus_tag="OO332_004341"
     CDS             complement(109..>484)
                     /locus_tag="OO332_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944523.1"
                     /translation="HILEHLRKLPRHEPVPGIMGKSGAGKSSLCNALFQGEVTPVSAL
                     MTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTGAVDRIFDTAESVCVASVVRTALR
                     AVRDTVVSVARAVWNWIFFLTR"
BASE COUNT          122 a          129 c          138 g           95 t
ORIGIN      
        1 attctattaa tatgaaaaac acaacgcaag ggggacaacc cgggtatggc tgcgtttgca
       61 ttgtgttttc atagagttaa aaatctgtaa cgacaacccg gcacatcact accgggttaa
      121 gaagaaaatc cagttccata ccgcgcgggc cacagagacc acggtgtcac gaacggcgcg
      181 cagggccgta cggacaacag aggcaacaca gacactctcc gccgtgtcaa atatccggtc
      241 caccgcaccg gtaaactgtt cacgggtctg agtgcggaca gactccgtgc gcagttcgtc
      301 ctgcaggcgt gtcatcagag ggatggcggc atgtccggga agcactgtca tgagtgcact
      361 gaccggggtg acctccccct gaaacagtgc attacagagt gaggatttac cggccccgct
      421 tttacccata atgccaggca cgggttcgtg acgggggagt ttgcgcagat gttccaggat
      481 gtga
//
LOCUS       DAJTRQ010000379          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000379 DAJTRQ010000000
VERSION     DAJTRQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.385"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..170
                     /locus_tag="OO332_004342"
     CDS             <1..170
                     /locus_tag="OO332_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944524.1"
                     /translation="KVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDS
                     KALREELETML"
     gene            167..>481
                     /locus_tag="OO332_004343"
     CDS             167..>481
                     /locus_tag="OO332_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR5944525.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSS"
BASE COUNT          127 a          113 c          136 g          105 t
ORIGIN      
        1 agaaagttga acagctgcgt aacacgctga ttcagagctc tgaagatcat aaacgcgatc
       61 agcaggcgca ggaacggctg accgggcgtc ttcaggcgtt aaatgaaagg gttcctgagc
      121 tcattactga cagtaaggcg ttaagggaag aactggagac aatgctgtga cttcaccatt
      181 tattcaacaa attgctgata accgggtatg tcaggtactc acctgtcttc ctgaaaaatt
      241 tgtggttgat ttcgcaaacg gtattgatgt tgcacaggag catatccgta cggccggaga
      301 gcgcacgttc ttccggcggt taaaagaggg gctgagcggc gaaggtactg cacggcagaa
      361 tgccatcaat gcttcgcttg cacagggcgt tgaggcgtcc ctgcgctggc tgacggagct
      421 gacaacgtca cttgccacca caaactacgc gattacccgg gtaaacgaca gggtcagttc
      481 a
//
LOCUS       DAJTRQ010000380          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000380 DAJTRQ010000000
VERSION     DAJTRQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.386"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          136 a           98 c           89 g          157 t
ORIGIN      
        1 taacaggatg ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt
       61 accctcatcg gtaaaaaaat tgcctttcga ccattacgat acattattga ttttggggtt
      121 tcagggaagt aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat
      181 ttgctactaa atagtttctt tattttctcc acgatgcctc caggtttata agtacaaacg
      241 gttatatcca catagagaca aaaatattaa tctgaaaaat atttatttca cgccgtatat
      301 ttgattgttt aatgtgcaag tacaatgact tttatttttt gttgtgtata taatcaaata
      361 tatggttatt tttcaccctg cgtattcagc acgcaacaaa aaacccgccg aagcgggttt
      421 agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg cgagggattt ctcccccgcc
//
LOCUS       DAJTRQ010000381          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000381 DAJTRQ010000000
VERSION     DAJTRQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.387"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>479
                     /locus_tag="OO332_004344"
     CDS             <1..>479
                     /locus_tag="OO332_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR5944526.1"
                     /translation="HLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGY
                     RAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLS
                     RVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELF"
BASE COUNT          118 a          100 c          160 g          101 t
ORIGIN      
        1 gcatttgcct gtcagcgtga aagtgcgtct gggctgggac agcggtgaga agaaatttga
       61 aatcgccgat gcggttcaac aggctggcgc tacggagctg gtggtgcatg ggcggacgaa
      121 agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga ttcgccagcg
      181 gctgaatatt ccggtgattg ccaacggtga aatctgggac tggcagagcg cgcaacaatg
      241 catggcgatc agcggctgcg acgcagtgat gattggtcgc ggggcgctca atattcccaa
      301 cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg tggccggagg tggttgcttt
      361 gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc gggttatatc acgttgcgcg
      421 gattaaacag tggttgagtt atttgcgtaa agaatacgat gaagcaacgg aattatttc
//
LOCUS       DAJTRQ010000382          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000382 DAJTRQ010000000
VERSION     DAJTRQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.388"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>473
                     /locus_tag="OO332_004345"
     CDS             <1..>473
                     /locus_tag="OO332_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5944527.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVML"
BASE COUNT           97 a          121 c          120 g          135 t
ORIGIN      
        1 ccggggctga aagccgttgg atcttatcgc tcggttctgg aattgctcaa tattccccat
       61 gcgaaattaa tcatggactg tgtgatatta ctttccgtaa ccagttgcct gaactcggcc
      121 ctgtataccg cctcaaggat gctctactcc ttaagccgtc gcggtgatgc tcccgcggta
      181 atgggtaaaa tcaaccgcag taaaaccccg tacgtggcgg tgttactctc caccggcgca
      241 gcattcttaa cggtggtggt gaactattac gcgcctgcga aggtatttaa atttctgatc
      301 gacagctccg gcgctatcgc cctgctggtt tatttagtca tcgccgtttc acagttgcgg
      361 atgcgcaaaa ttctgcgagc agaaggaagc gaaattcgct tgcgaatgtg gctttatccc
      421 tggctcacct ggctggtcat cggctttatt acctttgtgt tggtagtgat gct
//
LOCUS       DAJTRQ010000383          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000383 DAJTRQ010000000
VERSION     DAJTRQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.389"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..341)
                     /locus_tag="OO332_004346"
     CDS             complement(<1..341)
                     /locus_tag="OO332_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5944528.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVP"
BASE COUNT          121 a          137 c           95 g          116 t
ORIGIN      
        1 ggcacggaaa aatgctgctc ctcgccatta ctctctctca ctgtaacatc aagctccccg
       61 ccaacggcca gcgttggtaa attatccagc gcaaaaggcc ctggcggcac agaacgggta
      121 aaaataattt gcccattttg tttaataata acctgcgcct cactttgtgc tattccatgc
      181 accacaggtg tgtagtagct gtcctccggc ggagtcatcg catcgtcggt cgctaacatc
      241 ccgccgcgaa aaggtatgct gtcaaacacc tcagacgacg tataagattc tccaagcgtt
      301 aatcggcttt taatcgcatt tatgcctcgc tcaaaccaca tgtaacccct ttgccatctt
      361 tctgactggc caagttcctt gctccatgtc actgaactac gaaacctcca ggcaccaaga
      421 ttaaaccctg gagtgaacct gccataacga gaattacgcg tggtagatt
//
LOCUS       DAJTRQ010000384          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000384 DAJTRQ010000000
VERSION     DAJTRQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.390"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            50..>468
                     /locus_tag="OO332_004347"
     CDS             50..>468
                     /locus_tag="OO332_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5944529.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEG"
BASE COUNT          133 a          102 c          115 g          118 t
ORIGIN      
        1 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
       61 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
      121 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      181 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      241 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      301 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
      361 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      421 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggaggg
//
LOCUS       DAJTRQ010000385          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000385 DAJTRQ010000000
VERSION     DAJTRQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.391"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            31..447
                     /gene="torD"
                     /locus_tag="OO332_004348"
                     /pseudo
     CDS             31..447
                     /gene="torD"
                     /locus_tag="OO332_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT          115 a          108 c          130 g          113 t
ORIGIN      
        1 cactaaatca gtaagttggc agcattacct gccacgctga cagtacgtga cgatgcccgt
       61 ctggaactgg ccgctgactt ttgcggcctg tttctgatga ccgacaaaca agcggcgctg
      121 ccgtatgcat cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca
      181 gggatggaaa ccagtggcaa tttcaacgaa ccggcagatc atctggcgat ctatctcgag
      241 ttgttgagtt atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac
      301 agtttgcggc aaaaaatact gacggcgcta cggcaatggt taccagagtt tgctgcgcgt
      361 tgtcgacagt atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta
      421 gtggagtgcg accaccaaaa cagataacgt cgtttatgcg cctgaa
//
LOCUS       DAJTRQ010000386          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000386 DAJTRQ010000000
VERSION     DAJTRQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.392"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>464
                     /locus_tag="OO332_004349"
     CDS             <1..>464
                     /locus_tag="OO332_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5944530.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTRQ010000387          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000387 DAJTRQ010000000
VERSION     DAJTRQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.393"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>463
                     /locus_tag="OO332_004350"
     CDS             <1..>463
                     /locus_tag="OO332_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5944531.1"
                     /translation="RTTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIH"
BASE COUNT          118 a          121 c          115 g          109 t
ORIGIN      
        1 cgcaccactg gcagagcaga agcgaccatc caggtggata tcgctcatca gttctggcat
       61 attccgcatg aaaaaatctg gattgaagac cagtcaacaa actgcggtga aaacgcacgc
      121 tttagcatca cgctattgaa tcaggccgta gaacgagttc atacggctat cgttgtacag
      181 gaccccacca tgcagcggcg cacgatggca acgttccgcc gtatgaccgg ggacaatccc
      241 gatgcaccac gctggttaag ttatcccgga ttcgttcctc agttaggaaa taacgcagac
      301 agtgtaatct ttattaatca gttacaagga ttatggccgg ttgagcgtta tctctcacta
      361 ctcactggcg agctgccgcg tttacacgat gatagcgatg gctacggtcc ccgcgggcga
      421 gattttatcg ttcacgttga ttttccggca gaagtcatcc atg
//
LOCUS       DAJTRQ010000388          462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000388 DAJTRQ010000000
VERSION     DAJTRQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.394"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            97..288
                     /locus_tag="OO332_004351"
     CDS             97..288
                     /locus_tag="OO332_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5944532.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          117 a          110 c           87 g          148 t
ORIGIN      
        1 acgctttttt acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac
       61 cgtccacagc gtgggacata cttcaaggaa ccttttgtga gtcaggcaac tagtatgcga
      121 aaacgacacc gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc
      181 ttctttggcc gttttatcta ctcgtccgtc ggtgcctggc tgcaccatca gagcaaaaaa
      241 gaagctcagc aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct
      301 ccactttcac agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc
      361 aaagcggcag atattctttc atcttaaatt ttacgtcttt gtcctgactg atgtttatcc
      421 tgtttggctg cgaaataaat ataaaattaa tatatatgtt gt
//
LOCUS       DAJTRQ010000389          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000389 DAJTRQ010000000
VERSION     DAJTRQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.395"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>461
                     /locus_tag="OO332_004352"
     CDS             <1..>461
                     /locus_tag="OO332_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5944533.1"
                     /translation="AAKRAEDIADVVSLEDASTTKKGIVQLSSATNSASESLAATAKA
                     VKVVMDETNKKAPLNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDAL
                     NTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFT"
BASE COUNT          120 a          125 c          135 g           81 t
ORIGIN      
        1 cagcagcaaa acgggcagag gatattgctg atgtcgtatc tctggaagat gcgagcacga
       61 cgaaaaaggg gatagtgcag ctcagcagtg cgaccaacag tgcatccgag tcactggccg
      121 cgacagcgaa agcggttaag gtggtaatgg atgaaacaaa caagaaagcg cccctgaaca
      181 gtccggctct gaccggaacg ccaaccacac ccactgcgcc aaaggggact aataatactc
      241 agatcgccag tacggcttat gttatggccg cgattgctgc cctcgtggac tcgtcgcctg
      301 atgcactgaa cacgctgaat gaactggcgg cggcgctggg caacgacccg aattttgcga
      361 ccaccatgac taacgcgctt gcgggtaagc aaccgaaaga tgccactctg acggcgctgg
      421 cggggcttgc tactgcggca gacaggtttc cgtattttac g
//
LOCUS       DAJTRQ010000390          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000390 DAJTRQ010000000
VERSION     DAJTRQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.396"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(1..194)
                     /locus_tag="OO332_004353"
                     /pseudo
     CDS             complement(1..194)
                     /locus_tag="OO332_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(145..>461)
                     /locus_tag="OO332_004354"
     CDS             complement(145..>461)
                     /locus_tag="OO332_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5944534.1"
                     /translation="IVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSYRGS
                     RLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSGAMPGLFIR
                     TS"
BASE COUNT          106 a          130 c          117 g          108 t
ORIGIN      
        1 gctgacccgt cggtgcgaca tcatcagcgg tggctgtgta ggggactgcc tggtccctgt
       61 cgtcaaaaaa aaacgtcaca gtctactgcg ccactttcgg tataacgggg attaatgatt
      121 tttttaattt ccacggtgca ttcctcacga tgtgcgaata aaaagcccgg gcattgcgcc
      181 agagacatgt gcatccggca ccccggacag ggcgcaatat gaccccggta atgaatgctc
      241 tgaacatccc gtaatgaaaa attgtgggga tgctatatac gttccggtgg gagtactggg
      301 cactgaaatc cccaccggtc ccagtcgtga gcctctgtat gactgccccc tgacaagtct
      361 gatgacttta tcaccgtcag cttctccctg gtacgcagca ataatcagcc cgccaatgtc
      421 agggtctccc catctgttgc ggacagagct cgccacgatt c
//
LOCUS       DAJTRQ010000391          460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000391 DAJTRQ010000000
VERSION     DAJTRQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.397"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..70
                     /gene="relB"
                     /locus_tag="OO332_004355"
     CDS             <1..70
                     /gene="relB"
                     /locus_tag="OO332_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5944535.1"
                     /translation="LVEIVKERLRNPKPVRVTLDEL"
     gene            70..357
                     /gene="relE"
                     /locus_tag="OO332_004356"
     CDS             70..357
                     /gene="relE"
                     /locus_tag="OO332_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5944536.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          126 a           91 c          128 g          115 t
ORIGIN      
        1 acttgtggag atagtgaaag aacggcttcg taatcctaag ccagtacgtg tgacgctgga
       61 tgaactctga tggcgtattt tctggatttt gacgagcggg cactaaagga atggcgaaag
      121 ctgggctcga cggtacgtga acagttgaaa aagaagctgg ttgaagtact tgagtcaccc
      181 cggattgaag caaacaagct ccgtggtatg cctgattgtt acaagattaa gctccggtct
      241 tcaggctatc gccttgtata ccaggttata gacgagaaag ttgtcgtttt cgtgatttct
      301 gttgggaaaa gagaacgctc ggaagtatat agcgaggcgg tcaaacgcat tctctgaacc
      361 aaagcatgac atctctgttt cgcaccgaag gtgacacttc tgctttgcgt tgacaggaga
      421 agcaggctat gaagcagcaa aaggcgatgt taatcgccct
//
LOCUS       DAJTRQ010000392          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000392 DAJTRQ010000000
VERSION     DAJTRQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.398"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>450)
                     /locus_tag="OO332_004357"
     CDS             complement(<1..>450)
                     /locus_tag="OO332_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944537.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFS"
BASE COUNT          120 a          137 c          106 g           87 t
ORIGIN      
        1 gactgaaacc ttgttcggct tgcaaataac cgggtaacca cgcaatgtag agccaaccgg
       61 tataattgac gccagagaaa cctaaaatca tcccccaggt tgtccgatgc ttaaacagcg
      121 ccagccactc gctaaattgc agctgtggac gcggtttaac cggcgcggag agatacgtcc
      181 gttcttcgtc agtcaggaca aactgcgccc ggttgcgata ccacgcgtac caacagatcc
      241 caaccagaat ccctgccacg ccgatgataa cgaacatcgt ccgccagccc catgccagct
      301 gcatcaatac aagagcagga ggcgcgatag cctgaccgat aacggtagat gagttaaaaa
      361 tccccagcgc cgtgccgcgt tctttttgtg cataccagtc ggtgatcgac tttactcccg
      421 caggcataaa tggcgcttcg ccaataccca
//
LOCUS       DAJTRQ010000393          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000393 DAJTRQ010000000
VERSION     DAJTRQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.399"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(147..>450)
                     /locus_tag="OO332_004358"
     CDS             complement(147..>450)
                     /locus_tag="OO332_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5944538.1"
                     /translation="SLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGVGIRIFN
                     QSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          132 a           99 c           91 g          128 t
ORIGIN      
        1 tgctggttgg cctaaaaaaa cgctgagtat aatcgcggta aataacttac attaaactgt
       61 tgttttaaag agcctgatta aacaactttt tctggacata acgactccct ggccaaagcc
      121 aattgaataa aaaggccctt gataaattat ttcttatatt cgacagtaaa ttgtgaatca
      181 gcgttcgccg taccagccgt tacggtcgca ttggttgcca catagcgtgc aataaacttc
      241 atttcagcct tgccttcagc atcgatagcg gtataatcag agtcgttata aagtttaacc
      301 tgcgaattat cggactggtt aaaaatacga ataccaacgc cggttgccgc aatcgcttgt
      361 cctgtaccat caaaacgaac ggctgcttga gtgtacgttg ccgggcaatt ttcaagttta
      421 atactgaact tctgtggcga cgccagacta
//
LOCUS       DAJTRQ010000394          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000394 DAJTRQ010000000
VERSION     DAJTRQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.400"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>447)
                     /locus_tag="OO332_004359"
     CDS             complement(<1..>447)
                     /locus_tag="OO332_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HCR5944539.1"
                     /translation="VRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVF
                     VASYNAGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFT
                     VSDDGHLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVD"
BASE COUNT          110 a          135 c          115 g           87 t
ORIGIN      
        1 catccactga gctgtttaac tcattgacgc aatacgcata ttgttcgttt ggatggaata
       61 ccatatgacg cgggccggcc ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca
      121 gatgaccatc atcgctgacc gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc
      181 acagcgtacg gttgtccggt gagatattgg cggaatggca accgtccagc ccctcgacta
      241 catcgacgac gcccactggc aggccatctt ccagacgcgt tacgctcacg ttacccgcat
      301 tgtaagaagc tacaaagaca aactgcccct ggtgatcggt ggaaatatgc gtcggactac
      361 ccggcagcac agactctgcg gcaaaggtca gtgcgccatc gtccggggca atacgatacg
      421 ccaggacgcg aaactcaggg cgaacac
//
LOCUS       DAJTRQ010000395          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000395 DAJTRQ010000000
VERSION     DAJTRQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.401"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>442)
                     /locus_tag="OO332_004360"
     CDS             complement(<1..>442)
                     /locus_tag="OO332_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5944540.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYE"
BASE COUNT          120 a           91 c           85 g          146 t
ORIGIN      
        1 ttcataaata ccatactttg acaaaatctt tttacgagta tctgttggta atgattcaag
       61 cattttggct gtttgtaagg tttgctgcga aataccttgc aaatcagcct ggatggaatc
      121 atcggaagaa aaaatctcag gcataagagc ttcaaatcct ttctttctga acaactcaat
      181 cgcagaatgc agttccttgt cctctaacaa ttcgacaatt gcgatcagtt tagttttcaa
      241 tgtgctgcgg tcaggttgag aatttttatt ctcttgatta tttttatgct caaccattgg
      301 catgctctga ctttggataa accactctat tggacgccct gttgcgtagg ctatttgtgc
      361 taatcgatct aaagacgggt atgtcttgcc agacaggtag tttctgataa ccccatcaga
      421 aagtccgcac ttagctgcaa ac
//
LOCUS       DAJTRQ010000396          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000396 DAJTRQ010000000
VERSION     DAJTRQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.402"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..365)
                     /locus_tag="OO332_004361"
     CDS             complement(<1..365)
                     /locus_tag="OO332_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5944541.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSF"
BASE COUNT          107 a          107 c          108 g          115 t
ORIGIN      
        1 tcaaacgact ttagcaatgc cgtatcccgg gttctgcatg tcgcaactgt attaaggtat
       61 tgctctgcgg catcactcag ggctggagtg tgttcccgac ctactgattc gcacaggtaa
      121 tcaacgaagc ctgataccag ttttcgttcg cgaggcgtga tcgccccacc gaccggagtc
      181 cagtaatcaa atcccagttg caggagtttg aaaaaacgct tgtggaatgc gtagttacgc
      241 acacgcttaa agtctgcgtg tatccactca cctattttga tttgatgcaa aaaatcgcaa
      301 ctctccggcg tcgccgggag aagtaatccg gaagaggttt gtttgaccag ttgtatatgc
      361 gccatcgtag ttctccgctg gcgcagtaga atgggtgttc agcccgttat gtagtatacc
      421 agaattaatg ccaatac
//
LOCUS       DAJTRQ010000397          432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000397 DAJTRQ010000000
VERSION     DAJTRQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.403"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>432
                     /locus_tag="OO332_004362"
     CDS             <1..>432
                     /locus_tag="OO332_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5944542.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERR"
BASE COUNT          100 a          109 c          133 g           90 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacgtca tgctgggagc
      421 ggtggaacgc cg
//
LOCUS       DAJTRQ010000398          428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000398 DAJTRQ010000000
VERSION     DAJTRQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.404"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            228..>428
                     /gene="asr"
                     /locus_tag="OO332_004363"
     CDS             228..>428
                     /gene="asr"
                     /locus_tag="OO332_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HCR5944543.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNT"
BASE COUNT          137 a          102 c           85 g          104 t
ORIGIN      
        1 ctttgtaagt agtataatag ttaacccgat caagactact attattggta gctaaatttt
       61 ccttaagtca caatacgtta ttatcaacgc tgtaatttat tcagcgtttg tacatatcgt
      121 tacacgctga aaccaaccac tcacggaagt ctgccattcc cagggatata gttatttcaa
      181 cggccccgca gtggggttaa atgaaaaaac aaattgaggg tatgacaatg aaaaaagtat
      241 tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct gcagagactg
      301 cgaccacacc tgctccgact gcgacgacca ccaaagcagc gccagcgaaa actacacatc
      361 ataaaaaaca gcataaagca gcacctgccc agaaagcgca ggcggctaaa aagcatcata
      421 aaaatacg
//
LOCUS       DAJTRQ010000399          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000399 DAJTRQ010000000
VERSION     DAJTRQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.405"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>426)
                     /locus_tag="OO332_004364"
     CDS             complement(<1..>426)
                     /locus_tag="OO332_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5944544.1"
                     /translation="IVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYG
                     KPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGI
                     GYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQ"
BASE COUNT          115 a          108 c           95 g          108 t
ORIGIN      
        1 actggcggtt tgggtaatag acttggaagc ctggacgctc tgtgatccag tcttccagta
       61 cacattctaa acgaccagta tcaaggtact ccttgatctg ttcatagagc agatacccga
      121 taccagcacc catcaaaacg gcctccagtt cagcatcaac atcatccaga atgatatttc
      181 ctgcgacagc aatttccagc tcctttgcaa attgccagtg gaaaggtttg ccgctgggat
      241 aacgaaaaac aacacattga tgattaagca gatcatgagg atggcggggt ttgccatatc
      301 ttgcaaagta ctctggcgtt gctgctacac ataatttcac tggtgggccg atcgccacgc
      361 tgatcatatc tttttcgacg atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga
      421 cgatat
//
LOCUS       DAJTRQ010000400          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000400 DAJTRQ010000000
VERSION     DAJTRQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.406"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(42..>418)
                     /locus_tag="OO332_004365"
     CDS             complement(42..>418)
                     /locus_tag="OO332_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944545.1"
                     /translation="AILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFIAVV
                     RLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLALNVA
                     TPFVLVGLGLIFPLLARKANSK"
BASE COUNT          129 a          109 c           89 g           91 t
ORIGIN      
        1 cgggtcagat ctgccagagt cagcgtcacc gaccacaata attatttgct attcgcttta
       61 cgtgccagca gcgggaatat cagtcctaat cctaccagca caaacggcgt tgcaacattc
      121 agcgccaact ggaaggtcca ctctgcagtg aaggcttcca ttttcgggaa gatccccgtc
      181 aaacaggcac aggcggtaaa ggcaaaacac caaatcccga cggtcattgc cagaggctta
      241 ttacgaataa agacatattc tggtttatat ttctgcgcca ggcgaacgac tgcaataaat
      301 gcaacaaata cccataaata acgcagcggc atcactaccg agttaagatt caacagccat
      361 ttatagagat tgttcatatc accgatgccg agagtcggca gcataatcag aattgcca
//
LOCUS       DAJTRQ010000401          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000401 DAJTRQ010000000
VERSION     DAJTRQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.407"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            92..235
                     /locus_tag="OO332_004366"
                     /pseudo
     CDS             92..235
                     /locus_tag="OO332_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            246..>417
                     /locus_tag="OO332_004367"
     CDS             246..>417
                     /locus_tag="OO332_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--protein-cysteine
                     methyltransferase"
                     /protein_id="HCR5944546.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISAT"
BASE COUNT          100 a           95 c          106 g          116 t
ORIGIN      
        1 gaattatccc accctcgatc catgtgatgg cttgccctca tttcttcagg cagaaaaatt
       61 aaaaaatcgc tttatgctat cagactgcga caaaccagac gcgaaactgg gtacttacta
      121 ttcgttagtc ttgccctatc cacttatctt tttggtggta tggctgctga tgttgctggc
      181 gtggtatctt gtcggtctgc cgataggtcc gggtatttac ccacgtttgt cttaagagag
      241 aacggatgct gagattactt gaagaaaaaa ttgccacgcc actgggtcca ctgtgggtga
      301 tttgcgatga gcaatttcgc ctgcgggcgg ttgaatggga agagtacagc gaacgcatgg
      361 tgcagctgct ggacatccat tatcgcaaag aaggctatga gcgcatttct gccacca
//
LOCUS       DAJTRQ010000402          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000402 DAJTRQ010000000
VERSION     DAJTRQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.408"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(37..252)
                     /locus_tag="OO332_004368"
     CDS             complement(37..252)
                     /locus_tag="OO332_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5944547.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          130 a          102 c           94 g           91 t
ORIGIN      
        1 gtcgctgatg gggcgcttac cgtaatgatg gcgacattat tcacctctcg cagccttacg
       61 cttgtcttct ctgattttga agtacagatt tgtcagataa gtcaggaagc ccagaaccag
      121 actccccagt acaccaatcg cagcccactg tgacggactg acctgatcaa gccactgtaa
      181 aaaccagtag ccagcactgc ctgcggaggt gccgtaggca atgcccgttg aaattttgtc
      241 catggatttc atagcctcac ctccgcaaat aacggatggc gtagttttac actgagaaat
      301 gaaagggatt tgaaaagaaa aaaacgcaaa agcgggcgaa acgatatata cagtaaggaa
      361 agcactctat ccaacaaacc acccacagtt aatcggaata aaagcagagt gcttatg
//
LOCUS       DAJTRQ010000403          414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000403 DAJTRQ010000000
VERSION     DAJTRQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.409"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..316
                     /locus_tag="OO332_004369"
     CDS             <1..316
                     /locus_tag="OO332_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HCR5944548.1"
                     /translation="DRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYE
                     SRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKH
                     YQ"
     gene            333..414
                     /locus_tag="OO332_004370"
                     /pseudo
     CDS             333..414
                     /locus_tag="OO332_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
BASE COUNT          102 a           88 c          119 g          105 t
ORIGIN      
        1 tgacaggctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
       61 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
      121 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
      181 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
      241 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
      301 aaaacactat caataagttg gagtcattac ccgaatttat gttgaattta ctgttccatt
      361 taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttca cctg
//
LOCUS       DAJTRQ010000404          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000404 DAJTRQ010000000
VERSION     DAJTRQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.410"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(94..>413)
                     /gene="yqhC"
                     /locus_tag="OO332_004371"
     CDS             complement(94..>413)
                     /gene="yqhC"
                     /locus_tag="OO332_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5944549.1"
                     /translation="RVLKRIENKYTENLSVEQLAAEANMSVSAFHHNFKSVTSTSPLQ
                     YLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFGVTPGEDAARMRAM
                     QGN"
BASE COUNT           84 a          115 c           99 g          115 t
ORIGIN      
        1 ggcttgttag gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
       61 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
      121 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
      181 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
      241 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
      301 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
      361 ctcgacgctc aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggc
//
LOCUS       DAJTRQ010000405          412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000405 DAJTRQ010000000
VERSION     DAJTRQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.411"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            113..>412
                     /locus_tag="OO332_004372"
     CDS             113..>412
                     /locus_tag="OO332_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5944550.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQ"
BASE COUNT          140 a           62 c           81 g          129 t
ORIGIN      
        1 gattcctgtg gattctaatt ccagcggagc gttagttggt tatatgctta atgccaataa
       61 aattgcaacc gctgagataa caccagttta tgagataaag tagggtaaga gtatgctggc
      121 aaaagataag tctaacctaa agattgaaga aatacggatg cataaacatc atgagattca
      181 cagggttaag cctcttatgc cagctttatg tcgtatccgt cagggaaaaa aagttatcaa
      241 ttgggagacg catagtctaa ctgttgataa taatcagata atattatttc cctgtggata
      301 tgaattttat attgtgaatt atccagaagc aggtctttat cttgcagaaa tgctttatta
      361 tcccattgat ctaattgaaa agtttcaaaa attttatgcg ataactgatc aa
//
LOCUS       DAJTRQ010000406          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000406 DAJTRQ010000000
VERSION     DAJTRQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.413"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..319
                     /locus_tag="OO332_004373"
     CDS             <1..319
                     /locus_tag="OO332_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5944551.1"
                     /translation="GEAFSYVLNQWDALCYYSDDGLAEADNNTAERALRAVCLGKKNY
                     VFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDDLLPWKVVL
                     PSG"
BASE COUNT           95 a           95 c          116 g           91 t
ORIGIN      
        1 gggcgaagca ttcagctatg tactgaatca gtgggacgcc ctctgttatt acagtgatga
       61 cggtctggcg gaggcggaca ataacacagc ggaaagagcg cttcgtgcag tctgtctcgg
      121 aaagaaaaat tacgtgttct tcggtagcga tcacggcggc gagcgtggtg cactgctgta
      181 cgggctgatc ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat
      241 tctgagcgta ctgccggaat ggccttccaa ccgagttgac gacctcctgc catggaaggt
      301 ggtgctacca tcaggataat cgtcaatacg gtgttcggtg aacgtttact tggagcatca
      361 tgcttcatga agaaaaactg gcacgtcatc aacgtaa
//
LOCUS       DAJTRQ010000407          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000407 DAJTRQ010000000
VERSION     DAJTRQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.414"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            49..>397
                     /locus_tag="OO332_004374"
     CDS             49..>397
                     /locus_tag="OO332_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5944552.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDA"
BASE COUNT          115 a           80 c           95 g          107 t
ORIGIN      
        1 gtcatttaaa ggcattatca tatttacgaa gagatcagga tgatgacgat gaaaaaaagt
       61 gtattgacgg cgtttataac tgtggtatgt gcaacgtcca gcgttatggc tgctgatgat
      121 aatgctatca cggatggctc agtaacattt aatggtaaag ttattgctcc agcttgtacc
      181 ctggtagctg cgacgaaaga ttcagtagtc acattgccaa atgtcagcgc caccaagttg
      241 caaactaatg gagcagtctc tggcgttaaa actgatgtac caattgcctt agaaggctgt
      301 gatgttactg taactaaaaa tgcgacgttt acctttagtg gtacggctga cggcgtgcaa
      361 cctacagcat ttgccaacca ggctaccacc gatgcag
//
LOCUS       DAJTRQ010000408          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000408 DAJTRQ010000000
VERSION     DAJTRQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.415"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          129 a           77 c           79 g          109 t
ORIGIN      
        1 ccattcattc gctcgcgctg gcatctacag agatgcatgg tagagagcat aagaaatatc
       61 cacgccgaag ataaaatttt ctccatcttg atctaaccca ttaattattg agcataatgt
      121 aggcagtaca aaataagtta ggcgggatat caggcgtcaa gaatggagag cgagaactct
      181 ccattcttga cacctgatat tgcggacata ataagaaagc ataacgcctg aaatgctcac
      241 tttgcatcag catggtgata cagctgatgt ttattctaaa accttactca agttctaaga
      301 gagcacggat tccctgtcat taataatgat atccgttagt gtagtcttat gcatttcaat
      361 gtactgcaat atttgcagat agagatcaga aaga
//
LOCUS       DAJTRQ010000409          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000409 DAJTRQ010000000
VERSION     DAJTRQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.416"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>391
                     /locus_tag="OO332_004375"
     CDS             <1..>391
                     /locus_tag="OO332_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5944553.1"
                     /translation="VWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTM
                     KALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMER
                     FFRSLKNEWMPVVGYVSFSEAAHAITDY"
BASE COUNT           90 a           93 c          129 g           79 t
ORIGIN      
        1 ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt acctcgccgt
       61 tgttctcgac ctgttcgcaa gaaaaccagt gggctgggcc atgtcgttct cgccggacag
      121 caggctcacc atgaaagcgc tggaaatggc atgggaaacc cgtggtaagc ccggcggggt
      181 gatgttccac agcgatcagg gcagtcatta tacgagcagg cagttccggc agttattgtg
      241 gcgataccag atcagacaga gtatgagccg gcgcggaaac tgctgggata acagcccaat
      301 ggaacgcttc ttcaggagtc tgaagaacga atggatgccg gtggtgggtt acgtaagctt
      361 cagcgaggca gctcacgcca taacggacta t
//
LOCUS       DAJTRQ010000410          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000410 DAJTRQ010000000
VERSION     DAJTRQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.417"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(72..>387)
                     /locus_tag="OO332_004376"
     CDS             complement(72..>387)
                     /locus_tag="OO332_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5944554.1"
                     /translation="FSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLK
                     IFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKIT
                     YN"
BASE COUNT          113 a           80 c           86 g          108 t
ORIGIN      
        1 aggcaagaga gctggagtta gagcactgat ataacgggcc tgatggcccg ttttagtgtt
       61 tgtattagat gctagttata ggtgattttg aggattacgt ctttactaaa cggtccctca
      121 acaacagtct ctgccgggtt cgcactaagt tctgccctga atggtgcggt gaccgaaggt
      181 gcactagacg atgaaaaaga agcaaattgt gagaagcggt taaattccgt tgctgttcca
      241 tctttatgaa atatcttcaa tagcagtcca ttacccatat ccagatggct ataatcactc
      301 aaaatatccg tagtgaaaaa gctccctaaa atattgaatt gctcagtaca ggccgcacca
      361 ggatcttttg tcatgactac actaaac
//
LOCUS       DAJTRQ010000411          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000411 DAJTRQ010000000
VERSION     DAJTRQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.418"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(277..>387)
                     /gene="malG"
                     /locus_tag="OO332_004377"
     CDS             complement(277..>387)
                     /gene="malG"
                     /locus_tag="OO332_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="with MalKFE is involved in the transport of maltose
                     into the cell; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5944555.1"
                     /translation="GDFAAAAVMSALPITIVFLLAQRWLVNGLTAGGVKG"
BASE COUNT          133 a           76 c           93 g           85 t
ORIGIN      
        1 tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga tgaaaacaat
       61 actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa caaataacgt
      121 aaaaataata agttctatga tgggaatatt aaccggcgaa cgattcagat tgcagacgaa
      181 agaaaaaagg cgctccgtgg agcgccgaat aatagtcaca agttgggata acgtaagttg
      241 agggtgcagc ggcataacat tggcagaaca acatctttaa cctttcacac cacctgccgt
      301 caggccgttg accagccagc gttgagccag caagaagacg atggtgatcg gtaatgcaga
      361 catcacggca gcggcggcaa agtcacc
//
LOCUS       DAJTRQ010000412          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000412 DAJTRQ010000000
VERSION     DAJTRQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.419"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OO332_004378"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OO332_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR5944556.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           96 a           88 c          111 g           80 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc
      361 cgtaatggca atttg
//
LOCUS       DAJTRQ010000413          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000413 DAJTRQ010000000
VERSION     DAJTRQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.421"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            72..353
                     /locus_tag="OO332_004379"
     CDS             72..353
                     /locus_tag="OO332_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5944557.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT          128 a           79 c           82 g           82 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa g
//
LOCUS       DAJTRQ010000414          369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000414 DAJTRQ010000000
VERSION     DAJTRQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.422"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            44..>369
                     /gene="yfdR"
                     /locus_tag="OO332_004380"
     CDS             44..>369
                     /gene="yfdR"
                     /locus_tag="OO332_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5944558.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPP"
BASE COUNT          108 a           88 c           75 g           98 t
ORIGIN      
        1 accagaattc agcgcggtgc agcgcatata cgtggagaac aaaatgtcat ttattaaaac
       61 tttttccggg aagcattttt attatgacag gataaataaa gacgacatcg atattaacga
      121 tatcgcggtt tccctttcaa atatctgtcg ctttgccggt catctttcgc acttctacag
      181 cgtcgcccaa catgcggttc tttgcagcca gctggtgccg caggaatttg cttttgaagc
      241 gttaatgcat gatgcaacag aagcgtattg ccaggacatt cccgcaccac tgaaacgcct
      301 tcttcctgac tataaacaaa tggaagaaaa aatagactcc gtaatccgtg agaaatacgg
      361 attaccccc
//
LOCUS       DAJTRQ010000415          367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000415 DAJTRQ010000000
VERSION     DAJTRQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.423"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(235..>367)
                     /locus_tag="OO332_004381"
     CDS             complement(235..>367)
                     /locus_tag="OO332_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5944559.1"
                     /translation="AHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT          106 a           89 c           77 g           95 t
ORIGIN      
        1 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
       61 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
      121 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
      181 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
      241 catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt taccgggcga
      301 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
      361 atgggca
//
LOCUS       DAJTRQ010000416          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000416 DAJTRQ010000000
VERSION     DAJTRQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.424"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..167
                     /locus_tag="OO332_004382"
     CDS             <1..167
                     /locus_tag="OO332_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5944560.1"
                     /translation="FGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIESKHRP
                     KYIIKNEKQK"
BASE COUNT          113 a           67 c           69 g          114 t
ORIGIN      
        1 tctttggcaa cccagttcgc ggatatccct ccctgcttgt atcaatactt ttcttgggtg
       61 gagtgcagct gatcgggatt ggtgttctcg gtgaatatat aggcagaatc tatattgaaa
      121 gcaaacatcg tcccaaatac atcatcaaaa atgagaaaca aaaatgaaaa gccttaaaac
      181 atcatatgta aaaaaactat ttttcttaat tgctgtattt ttcattgcgg ttattttgat
      241 gtatttgcgc cgccctgata taatcagaac accgcagttt tgggcagaag atggtcatgt
      301 atggtatgct atggcataca ataatggcat attcacatca atgatattcc ctcaaaatgg
      361 ata
//
LOCUS       DAJTRQ010000417          362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000417 DAJTRQ010000000
VERSION     DAJTRQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.425"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..336
                     /locus_tag="OO332_004383"
     CDS             <1..336
                     /locus_tag="OO332_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR5944561.1"
                     /translation="HLGLSAPGDRTAAKIEKLLAWLETLKAELGIPKSIREAGVQEAD
                     FLANVDKLSEDAFDDQCTGANPRYPLISELKQILLDTYYGRDYVEGETAAKKEAAPAK
                     AEKKAKKSA"
BASE COUNT          104 a           87 c           98 g           73 t
ORIGIN      
        1 cacctgggtc tgagcgcacc gggcgaccgt actgctgcta agatcgagaa actgctggca
       61 tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga agctggcgtt
      121 caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcatt cgatgaccag
      181 tgcaccggcg ctaacccgcg ttacccgctg atctccgagc tgaaacagat tctgctggat
      241 acctactacg gtcgtgatta tgtagaaggt gaaactgcag cgaaaaaaga agccgctccg
      301 gctaaagctg agaaaaaagc gaaaaaatcc gcttaatcag tagcgctgtc tggcaatata
      361 aa
//
LOCUS       DAJTRQ010000418          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000418 DAJTRQ010000000
VERSION     DAJTRQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.426"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..221)
                     /locus_tag="OO332_004384"
     CDS             complement(<1..221)
                     /locus_tag="OO332_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5944562.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVG"
BASE COUNT          104 a           84 c           74 g           99 t
ORIGIN      
        1 cccaccactt ctgtttctgg cgaaacgctg gcgatatcgc gtttaagcgt ttcaaatagc
       61 gagcgcgagc gggcatcgtg caataattcc agtcgatcga tgaccaacga ttcttgctgg
      121 catatacaga gtgacggacg ccattttgac cagcgctgac tgccgcgtcc ggcataatcc
      181 agcacggtac cgacaaagcc aaaagccact gttttacgca tcttagatat ccttataaaa
      241 ggcgatattt cattatcggt aaatcggctt tgcgggaaat ctccgtttac ggacataaag
      301 atataatctt tttagaaaca ataaattaaa aaattaatct gcttatttaa ttttctggca
      361 c
//
LOCUS       DAJTRQ010000419          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000419 DAJTRQ010000000
VERSION     DAJTRQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.427"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..230
                     /locus_tag="OO332_004385"
     CDS             <1..230
                     /locus_tag="OO332_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944563.1"
                     /translation="YPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWEQD
                     LTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT           88 a           82 c           92 g           95 t
ORIGIN      
        1 catatccggt acaggacgat gcacactggc tggaacgtta tcagcaactc tggagccagc
       61 atcgtgaccg tgttatcggt acccaggaac tggtagcgat tgtgttggca gaaaaagacc
      121 actgggagca ggacctgacg caagtgcctg gtctggtcga gcaggttgct aatgacctgg
      181 atgcgattct tgaaaaaggg atgcgcgaag ctgtacgtcc gttgtgctaa taacaccgtt
      241 acttctgaac ccggtttcga ccgggtttct tatttcacta tattagttac aacatcctat
      301 atgcctttat cattaacagg tgtcacatgc agcgcctgtt aatgttgtaa gcttgac
//
LOCUS       DAJTRQ010000420          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000420 DAJTRQ010000000
VERSION     DAJTRQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.428"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..336
                     /locus_tag="OO332_004386"
     CDS             <1..336
                     /locus_tag="OO332_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5944564.1"
                     /translation="FSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERAN
                     EIKHPVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSM
                     QTHKYLNIA"
BASE COUNT          105 a           82 c           88 g           81 t
ORIGIN      
        1 tttagctgcc agatcgaaac cagtggaact catgaagtac gctgcacacc gaatacctgg
       61 gttaccgtat cgccaaagct gaacatgcgc ggcggctatg aagtgttgtc acaggcactg
      121 gagcgagcca acgaaatcaa gcatccagtg gggcgcgtac gcgatattga agcactggat
      181 gaactactgg caacgctgac cgatgataaa ccgcgagtca ttgcactgca gccgattagc
      241 caaaaggatg atgccacacg attgtgcatt gaaacctgta ttgcgcgtaa ttggcgtttg
      301 tcgatgcaaa cacataaata tttaaatatt gcctgattac acatttataa gcgtta
//
LOCUS       DAJTRQ010000421          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000421 DAJTRQ010000000
VERSION     DAJTRQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.429"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          124 a           60 c           60 g          110 t
ORIGIN      
        1 tacagacata ttctggtgcg aggaaaagaa accgaaacca tttcagaaag gttgatacgt
       61 gaggctgata cacgagaata tgaactatgg cgtccggtcc gtcgcttttt ctccattgtt
      121 ttcctcattg ttctattagg ttgcattcat tcactgataa caactataaa ataaaagtta
      181 tggctgttct gatacaggaa aataaacgtt cattctatgg ttacctcttc ctgttatata
      241 gaagaatttt aaacgacaag taagggaata taaatgaaaa taacaaaaaa taaaaatgca
      301 actataaaaa acagtcatca gtcggctgct tttcaatgtt taccttatca tttt
//
LOCUS       DAJTRQ010000422          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000422 DAJTRQ010000000
VERSION     DAJTRQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.431"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>351
                     /locus_tag="OO332_004387"
     CDS             <1..>351
                     /locus_tag="OO332_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5944565.1"
                     /translation="PSSLQSLSALLNSLETLPDLPPALQKLSVGNNQLTALPELPCEL
                     QELSAFDNRLQELPPLPQNLRLLNVGENQLHRLPELPQRLQSLYIPNNQLNTLPDSIM
                     NLHIYADVNIYNNP"
BASE COUNT          106 a          100 c           51 g           94 t
ORIGIN      
        1 tccatcttcc ttgcagtcac tctcagccct tctcaatagc ctggagacgc tacctgatct
       61 tcccccggct ctacaaaaac tttctgttgg caacaaccag cttactgcct taccagaatt
      121 accatgtgaa ctacaggaac taagtgcttt tgataacaga ttacaagagc taccgcccct
      181 tcctcaaaat ctgaggcttt taaacgttgg ggaaaaccaa ctacacagac tgcccgaact
      241 tccacaacgt ctgcaatcac tatatatccc taacaatcag ctgaacacat tgccagacag
      301 tatcatgaat ctgcacattt atgcagatgt taatatttat aacaatccat t
//
LOCUS       DAJTRQ010000423          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000423 DAJTRQ010000000
VERSION     DAJTRQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.432"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..348
                     /locus_tag="OO332_004388"
     CDS             <1..348
                     /locus_tag="OO332_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="catalyzes the formation of AMP from
                     adenosine-3',5'-bisphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5944566.1"
                     /translation="WKEECGVRKLIQVRDARPPLVVISRSHADAELKEYLQQLGEHQT
                     TSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGAHVHDWQGKPLDYTPR
                     ESFLNPGFRVSIY"
BASE COUNT           73 a           97 c          108 g           72 t
ORIGIN      
        1 tggaaagaag agtgcggtgt gcgcaagctg attcaggtcc gcgatgcgcg cccgccgctg
       61 gtggttatca gccgttccca tgcagatgcg gagctgaaag agtatctgca acagcttggc
      121 gaacatcaga ccacgtccat cggctcttcg ctgaaattct gcctggtggc ggaaggacag
      181 gcgcagctgt acccgcgctt cggaccaacg aatatttggg acaccgccgc tggacatgct
      241 gtggctgcag ctgccggagc gcacgttcac gactggcagg gtaaaccgct ggattacact
      301 ccgcgcgagt cgttcctgaa tccggggttc agagtgtcca tttactaaat
//
LOCUS       DAJTRQ010000424          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000424 DAJTRQ010000000
VERSION     DAJTRQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.434"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>349
                     /locus_tag="OO332_004389"
     CDS             <1..>349
                     /locus_tag="OO332_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR5944567.1"
                     /translation="EHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLV
                     NHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEALYHAGALKTRQDVTEAL
                     TAAGFEVVRTTRSS"
BASE COUNT           92 a           92 c          109 g           56 t
ORIGIN      
        1 ggagcaccag gacaaggggc gtcttgagct gaattttgtc atcccgaata tggagctggc
       61 gagcggaaaa cggctccagc cctattacga ccgagcggac cgtccgcgaa tcaatgcctg
      121 gcagacactg gtgaatcacc attacgggct gcacgacccg aacgcaccgg agaacaggcg
      181 gacacttgtc accccgaata atctgccgaa agcgaaacag gaggccgcag aagcgattac
      241 gaggggactg gaggctcttt accatgccgg agcgctaaaa acgcgtcagg atgtcactga
      301 ggcgcttaca gcggcaggtt ttgaggttgt gaggacaacc aggagcagt
//
LOCUS       DAJTRQ010000425          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000425 DAJTRQ010000000
VERSION     DAJTRQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.435"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            24..100
                     /locus_tag="OO332_004390"
     tRNA            24..100
                     /locus_tag="OO332_004390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OO332_004391"
     tRNA            143..218
                     /locus_tag="OO332_004391"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           72 c           87 g           89 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagt
//
LOCUS       DAJTRQ010000426          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000426 DAJTRQ010000000
VERSION     DAJTRQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.436"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            45..>345
                     /locus_tag="OO332_004392"
     CDS             45..>345
                     /locus_tag="OO332_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5944568.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT           94 a           96 c           79 g           76 t
ORIGIN      
        1 gtggcagtga aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca
       61 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggtatta
      121 cgcctgtggc acaggcactc gccgccgaag ggcaagctaa cgcggatgac acgctggttg
      181 tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc
      241 gtccggtagc ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc
      301 agggcgcaac caaccttacc gacgcgctca aaaacgtccc cggcg
//
LOCUS       DAJTRQ010000427          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000427 DAJTRQ010000000
VERSION     DAJTRQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.437"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>345
                     /locus_tag="OO332_004393"
     CDS             <1..>345
                     /locus_tag="OO332_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5944569.1"
                     /translation="VVSQLALACTLAITSQANAATNDISGQTYNTFHHYNDATYADDV
                     YYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNANSLIIKNST
                     IHGMIYSECMTT"
BASE COUNT          100 a           86 c           62 g           97 t
ORIGIN      
        1 tgtagtatca caattagcat tggcttgcac tctggctatc acctctcagg ctaatgcagc
       61 gaccaacgat atttctggtc aaacttacaa tactttccat cactacaacg acgccaccta
      121 tgctgatgac gtttactatg atggttatgt aggctggaac aactatgccg ctgatagcta
      181 ttacaacggc gatatctacc cggtcattaa taacgctacc gttaacggcg tgatttctac
      241 ctactatctg gacgacggta tttctaccaa taccaacgcc aatagtctga taatcaaaaa
      301 cagcactatt cacggtatga tttattccga gtgcatgaca actga
//
LOCUS       DAJTRQ010000428          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000428 DAJTRQ010000000
VERSION     DAJTRQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.438"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT           85 a           70 c           89 g          100 t
ORIGIN      
        1 attgttagtc tttgcatgag caagcggact gacacatttc tctttgttct cattcagaaa
       61 atctcatcag tcggcgttca gaccggtgtt gtgctttatg gatacgttac gctcctgatg
      121 acgtcatttg acgttcaaca gcatcacggg gccgcacgac atttcacgtc agttagtgct
      181 atagctcagg aacaaatttt cccaaattgg gatatgctcg caaattgctg gtgatgtggg
      241 gagaatctgg ttgagttcgg tagaattgat ttggaggcag aacgcttaaa tcgtggcgtc
      301 ctgaaacgaa aaacggacct ccgtggaggt ccgttgatat gaat
//
LOCUS       DAJTRQ010000429          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000429 DAJTRQ010000000
VERSION     DAJTRQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.439"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>344
                     /locus_tag="OO332_004394"
     CDS             <1..>344
                     /locus_tag="OO332_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5944570.1"
                     /translation="ATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLK"
BASE COUNT          105 a           65 c           80 g           94 t
ORIGIN      
        1 ggctacgcgg ccaaacgaga agtgggttac cgatgttact gaatttgcag tcaatgggcg
       61 caagctgtat ttgtctccag taatagatct cttcaacaac gaagttattt cttacagcct
      121 ttcggaaaga ccagtgatga acatggttga gaatatgctc gatcaggcat tcaaaaagct
      181 taatcctcac gagcatcctg ttctgcactc tgaccaggga tggcagtatc gtatgagaag
      241 atatcaaaat atccttaaag aacatggtat taaacaaagc atgtccagaa aaggcaattg
      301 tctggataat gctgtggtgg agtgtttctt tggaacctta aagt
//
LOCUS       DAJTRQ010000430          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000430 DAJTRQ010000000
VERSION     DAJTRQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.440"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..311
                     /locus_tag="OO332_004395"
     CDS             <1..311
                     /locus_tag="OO332_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5944571.1"
                     /translation="KQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWS
                     LAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG
                     "
BASE COUNT          118 a           77 c           73 g           75 t
ORIGIN      
        1 taaaacagcg gcagttttcc ggaccgcact ataaggttga ctccatcagg gagtcatgga
       61 caagtatctt aaaacgcgca ggtctgagac acagaaaatc gtatcaatcc aggcatacct
      121 atgcatgctg gtcacttgcc gcaggagcta atcctagttt tatcgcaagc cagatgggcc
      181 acacaaacgc acaaatggta ttcaatgttt acggagcatg gatgaaagac aacaatcacg
      241 aacagataga acttcttaac agaagactat ctgaaagtgt cccatgcatg ccccataaga
      301 aagcggggta aaataaaaac ttgcaaaatc aattggttta cct
//
LOCUS       DAJTRQ010000431          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000431 DAJTRQ010000000
VERSION     DAJTRQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.441"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>343
                     /locus_tag="OO332_004396"
     CDS             <1..>343
                     /locus_tag="OO332_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR5944572.1"
                     /translation="SRQWPGDGGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRY
                     DARSVSTGIAGRSLTADLIDCAAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVI
                     PDVQPDHGETVI"
BASE COUNT           67 a           88 c          115 g           73 t
ORIGIN      
        1 tcccgccagt ggccgggaga tgggggtatc accacgcttc agccgcgcat taaaaacggt
       61 tcaaaagtgg aagtgctgat tggtgatgag ctggtgatca ccggctgggt ggaggcgacg
      121 cccgttcgtt acgatgcccg ttcggtcagc accggtattg ccggacgcag tctgacggct
      181 gacctgattg actgtgcagc cgaaccgaca cagtttaacg gacgagagct ggtacagatt
      241 gcgcaggcgc ttgctgcgcc tttcggcatt gaggtggtga acaacggtgc gccgtcgggt
      301 gttattcctg acgtccagcc cgatcacggt gaaacggtga ttg
//
LOCUS       DAJTRQ010000432          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000432 DAJTRQ010000000
VERSION     DAJTRQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.442"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>337
                     /locus_tag="OO332_004397"
     CDS             <1..>337
                     /locus_tag="OO332_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5944573.1"
                     /translation="LASVSLRTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHL
                     KTIVEWELSVSRDDLNDPESYQLYVDQYDDIKSKLREAVATERLTVDQHRYINDVIGV
                     LKACQDRLNG"
BASE COUNT           93 a           86 c           83 g           75 t
ORIGIN      
        1 aactagcgtc agtatcttta cggaccaagg atagacgtac cgctatggct cactcacggc
       61 acatcaagtc agcacttaaa gcaatccatg cagacaaccc taacgcctct tatgaggagc
      121 tacgggagca cctgaagacc attgtcgagt gggaacttag tgtcagccgt gatgacctga
      181 acgacccgga gtcctaccag ctttacgttg accagtacga tgacattaag tccaaacttc
      241 gggaagctgt agcgaccgag cgtctgactg tagaccagca tcgctatatt aatgacgtta
      301 tcggtgtgct taaggcctgt caggatagac tcaatgg
//
LOCUS       DAJTRQ010000433          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000433 DAJTRQ010000000
VERSION     DAJTRQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.443"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(64..337)
                     /locus_tag="OO332_004398"
                     /pseudo
     CDS             complement(64..337)
                     /locus_tag="OO332_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           87 a           82 c           73 g           95 t
ORIGIN      
        1 actatcatac cctttatcct tgctgaatcg aagcagcagc aagatgattc tgaagttcag
       61 gaattatttg cgcaaggccc ggcaaatcag attcgatagc aactcaagct gatgtgccgt
      121 ttccatgttc agccacacat aaccatagat tggtgtttcc acgactttca ggtcatggtg
      181 gttatcgagc aactgacgca gctcttccac agcatcgttc aatttttccg tattggcaaa
      241 aaccggctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat
      301 catgtgctgg gtatcacgaa gtttttacgc gttcaat
//
LOCUS       DAJTRQ010000434          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000434 DAJTRQ010000000
VERSION     DAJTRQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.444"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>335
                     /locus_tag="OO332_004399"
     CDS             <1..>335
                     /locus_tag="OO332_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944574.1"
                     /translation="LMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAKTGYIWTY
                     VRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLFSAEREGG
                     ALTEAGCWA"
BASE COUNT           83 a           83 c          102 g           67 t
ORIGIN      
        1 agttaatgac gccgctgaat gatgctctgt accgttatgt aatgaacagc cgcaaagttc
       61 acactgatga cacaccagta aaagtgctgg caccgggcag gaagaaggcg aaaacaggat
      121 atatctggac gtatgtccgg gatgacagga atgccggttc gccagagcct ccggcggtct
      181 ggttcgccta ctcaccggac catcagggta aacatccgga gcagcacctt agtcccttcc
      241 ggggtatcct gcaggcagat gcgtttaatg gttacgatcg gctgttcagt gccgaacgag
      301 aaggcggcgc gttgacggaa gcaggatgct gggct
//
LOCUS       DAJTRQ010000435          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000435 DAJTRQ010000000
VERSION     DAJTRQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.445"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..293)
                     /gene="ynaI"
                     /locus_tag="OO332_004400"
     CDS             complement(<1..293)
                     /gene="ynaI"
                     /locus_tag="OO332_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5944575.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLL"
BASE COUNT          115 a           65 c           61 g           93 t
ORIGIN      
        1 atcagcagaa atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg
       61 ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt aatgatgcta
      121 ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa taaaaatcct
      181 tttcttttac gatttccacg gcgaaaccaa aagctcatca gaatcaatgc tgcgcagcta
      241 ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat cattgtttta
      301 tcaggctcct ccagataatt gtcgtcatgc cgga
//
LOCUS       DAJTRQ010000436          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000436 DAJTRQ010000000
VERSION     DAJTRQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.446"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..215)
                     /locus_tag="OO332_004401"
     CDS             complement(<1..215)
                     /locus_tag="OO332_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944576.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKW"
BASE COUNT           75 a           80 c           86 g           92 t
ORIGIN      
        1 caccacttcg ggtcatccgc cttcatctcc gcttctgttt cgtataaatc aaaacgacgg
       61 caggtatggc agaacgtgac gtattcgtac ggtgaatccg ggtacatttt gtggaaggtt
      121 tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa tacggactta
      181 taagggtgat tattggctct ggcgacggct ttcagaaccc tctttcacca ttctggtgtg
      241 gactttctgg tgctccaggt tgtgtgacat gggaactcat tctggatggt tactctgaaa
      301 gctcatattc tgccacaccc cgatttgcag ccg
//
LOCUS       DAJTRQ010000437          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000437 DAJTRQ010000000
VERSION     DAJTRQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.447"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(57..>333)
                     /locus_tag="OO332_004402"
     CDS             complement(57..>333)
                     /locus_tag="OO332_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5944577.1"
                     /translation="RLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEIISTLFANKV
                     DVDHPENKYKDFKDLEDKVEKRLQNCQAKYQNEINQLSALGVNFDDI"
BASE COUNT          103 a           56 c           45 g          129 t
ORIGIN      
        1 cactgactat ttccgatatt caggcgttga aaaatcaaaa aactggaaaa ataattttaa
       61 atatcatcaa aattaacacc tagtgcagat agttgattaa tttcattttg atatttcgcc
      121 tggcaatttt gaagtctttt ttcaacttta tcttcgagat ccttaaagtc tttgtattta
      181 ttttcaggat ggtcaacgtc aactttgtta gcgaataaag tagaaattat ttcttttaca
      241 ttatcgcgtc taaattttgc atcgtccact tgaaaattat atcttattgc tcgacctaaa
      301 cgtttaaaaa cttcgttagg ttctctcagt ctt
//
LOCUS       DAJTRQ010000438          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000438 DAJTRQ010000000
VERSION     DAJTRQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.448"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..272
                     /locus_tag="OO332_004403"
     CDS             <1..272
                     /locus_tag="OO332_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5944578.1"
                     /translation="KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSR
                     YSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
BASE COUNT          106 a           80 c           63 g           82 t
ORIGIN      
        1 taaagggata cgattacgat acatatacct tctatgccaa aaaaggtcag aaagtgcatg
       61 tgagtatctc taatgaaggc gctgatactt atctcttcgg gccaggaatc gatgattctg
      121 ttgatttatc cagatattcc ccagaactcg atagtcacgg acaatactca ctccctgctt
      181 ctggcaaata tgagctgcga gtgctgcaaa ctcgaaacga cgcccgtaaa aacaaaacta
      241 aaaaatataa cgtcgatatt caaattaagt aaatgtcaac cgacggggac tatccccgtc
      301 atagacgtta acgccccact cctctctgtc g
//
LOCUS       DAJTRQ010000439          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000439 DAJTRQ010000000
VERSION     DAJTRQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.450"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..244
                     /locus_tag="OO332_004404"
     CDS             <1..244
                     /locus_tag="OO332_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5944579.1"
                     /translation="DDPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRES
                     FCVDRITPDDGGCSYIRLRREGLPGNVRAGRYYEGA"
     gene            231..>330
                     /locus_tag="OO332_004405"
     CDS             231..>330
                     /locus_tag="OO332_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5944580.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQK"
BASE COUNT           76 a           68 c           99 g           87 t
ORIGIN      
        1 tgatgatcct gaaaatattt ctctggtggc cggcggtgtg cgtattgaag actctttacc
       61 atccctgttt gtgaaaacag cagatatttt acggctgtgt cgcaatgatt cgctgatgat
      121 tagtcgtgag tctttctgtg tggatcgtat cacccctgat gatggcggat gtagttatat
      181 ccggttgcgg cgtgaggggc tgccgggaaa cgtaagggca ggacgatatt atgaaggggc
      241 ttgagaatgc catccgcaat ctgaacagcc ttgatacccg tatggtgcca caggccagcg
      301 catgggcgat aaaccgtgtg gcacagaaag
//
LOCUS       DAJTRQ010000440          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000440 DAJTRQ010000000
VERSION     DAJTRQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.451"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>329
                     /locus_tag="OO332_004406"
     CDS             <1..>329
                     /locus_tag="OO332_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HCR5944581.1"
                     /translation="RHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLEL
                     VSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLY
                     RQSEIFAR"
BASE COUNT           82 a           85 c           98 g           64 t
ORIGIN      
        1 cgtcatcgtc gcccgttacc ggagcatctg ccccgcgaaa taaatcgcct ggagccagaa
       61 gaaagctgtt gcccggagtg tggcggtgag ctggattatc tgggggaagt cagcgcagaa
      121 caactggaac tggtgagcag cgctctgaaa gtgatccgca cagaacgggt aaaaaaagcc
      181 tgtacaaaat gtgactgcat cgttgaagca ccggcaccat cccgtccgat agagcgtggt
      241 atcgcgggcc cggggttact tgcccgcgtg ttaacgggaa aatactgcga acacctgcca
      301 ctgtatcgtc agagtgaaat ttttgcccg
//
LOCUS       DAJTRQ010000441          328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000441 DAJTRQ010000000
VERSION     DAJTRQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.452"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..>328
                     /locus_tag="OO332_004407"
     CDS             <1..>328
                     /locus_tag="OO332_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5944582.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSR"
BASE COUNT          109 a           62 c           71 g           86 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccg
//
LOCUS       DAJTRQ010000442          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000442 DAJTRQ010000000
VERSION     DAJTRQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.453"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>327)
                     /gene="yqhC"
                     /locus_tag="OO332_004408"
     CDS             complement(<1..>327)
                     /gene="yqhC"
                     /locus_tag="OO332_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5944583.1"
                     /translation="YDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQELLM
                     DIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREILYYV
                     LTGPCG"
BASE COUNT           80 a           97 c           79 g           71 t
ORIGIN      
        1 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
       61 tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg aataaaatct
      121 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
      181 cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
      241 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
      301 gcagcaggta ttcattggca tcataac
//
LOCUS       DAJTRQ010000443          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000443 DAJTRQ010000000
VERSION     DAJTRQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.454"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            43..327
                     /locus_tag="OO332_004409"
                     /pseudo
     CDS             43..327
                     /locus_tag="OO332_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768536.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          106 a           51 c           79 g           91 t
ORIGIN      
        1 gaccaggaat gtttgttaat ccaatgaatg gatggttaat ctatgaaact gaaaaaactc
       61 cccgggttta gtttgggact tattgctctg gcggtgggta atgcatatgc tgaacagttt
      121 attcagcacg atgaatataa acatgctgtg gcaataaaag atggaaaagt aaaagatatt
      181 gataatgcgc tagttataac aacgggtaat ggaagttacg ggtatccgtt gcaggagagg
      241 gtagtaagtt aaccattaac aatggcgtca ttgtaacaac gggcggttta tatccttctg
      301 acaatagtcc tctaagccca gcattaa
//
LOCUS       DAJTRQ010000444          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000444 DAJTRQ010000000
VERSION     DAJTRQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.455"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>325)
                     /locus_tag="OO332_004410"
     CDS             complement(<1..>325)
                     /locus_tag="OO332_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5944584.1"
                     /translation="NWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGGIFY
                     RSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTWNGP
                     GWSDRS"
BASE COUNT           90 a           88 c           66 g           81 t
ORIGIN      
        1 tgacctgtca gaccagccgg gaccattcca tgtccgaaca gatgacagac caccaaggcg
       61 aatacctgta ataaaccttg agttacattc tgcctgcgta tatgcgccaa catctctcgc
      121 tgatggcttg cgtgttgtgg tgtaaaactc tgaccagcca gcctcaaatc cataaccatc
      181 acgcgctgaa cgataaaaaa taccgccgtt cttataattc acacggaact gtacagcagg
      241 gcagctcccc gtattcttat tgaagtggag gattaatgtt gatgcaccgc tgatatttgc
      301 atcataaaca ccgctattcc agttc
//
LOCUS       DAJTRQ010000445          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000445 DAJTRQ010000000
VERSION     DAJTRQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.456"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT          101 a           67 c           83 g           70 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt c
//
LOCUS       DAJTRQ010000446          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000446 DAJTRQ010000000
VERSION     DAJTRQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.457"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(218..>321)
                     /locus_tag="OO332_004411"
     CDS             complement(218..>321)
                     /locus_tag="OO332_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5944585.1"
                     /translation="CCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT           89 a           87 c           84 g           61 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tgcgcgtgag gaatatagcc ccggataaca gattgagtca acaaacggtt tttagatccc
      241 cggaagagaa tgcatcacgc atcggtagat agagcatata ttcagcaaga tttagccata
      301 catctacccg actgcagcac g
//
LOCUS       DAJTRQ010000447          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000447 DAJTRQ010000000
VERSION     DAJTRQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.458"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..>318)
                     /locus_tag="OO332_004412"
     CDS             complement(<1..>318)
                     /locus_tag="OO332_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5944586.1"
                     /translation="WLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISS
                     ILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVI
                     HNC"
BASE COUNT          105 a           73 c           68 g           72 t
ORIGIN      
        1 cacagttatg aatcacaatc agtgcaacca taatggtgct cggggcatag cttttatcta
       61 cgatgataga aagcattggc cacgccagca caatcgcaga ggtattcata ataatatacg
      121 ggatccggcg accaatttta tcggataacc aaccaaggaa cggaatggtc ataaagccga
      181 gaatcgaact gatcatcaat gcatctgttg gaattgcttt gttaaacaat aacgtctgta
      241 ctaaatagcc tgcaaggaaa gtctgaatta aaccggagtt acccgcctga ccaaaacgca
      301 gccctgttgc cagccaga
//
LOCUS       DAJTRQ010000448          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000448 DAJTRQ010000000
VERSION     DAJTRQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.459"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT           92 a           67 c           84 g           67 t
ORIGIN      
        1 aacgaaaaat gggagcggat gttacagagt tctcggtaca ggggaaaagt ggtacctgtc
       61 gccagttctc cgatctgttt aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg
      121 tgatggagca tcgttaatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct
      181 gcatatggat cagggttggc aatatcgaat cgcaggctat aggcaaagtt aaagccccat
      241 ggagtagcac aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac
      301 ttcttcagca
//
LOCUS       DAJTRQ010000449          307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000449 DAJTRQ010000000
VERSION     DAJTRQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.460"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            4..307
                     /locus_tag="OO332_004413"
                     /pseudo
     CDS             4..307
                     /locus_tag="OO332_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           84 a           73 c           84 g           66 t
ORIGIN      
        1 cagatgattg atgtcttagg accggagaaa cgcagacggc gtactacaca ggaaaagatc
       61 gctattgttc agcagagttt tgaaccggga atgacggtct cccttgttgc ccggcaacac
      121 ggtgtggcag ccagccagtt atttctctgg cgcaagcaat actaggaggg aagtcttact
      181 gctgtggccg caggagaaca ggtcgttcct gcctctgaac ttgctgctgc catgaagcag
      241 attaaagaac tccagcgcct gctcggcaaa aaaacgatgg aaaatgaact ccttaaagaa
      301 gccgttg
//
LOCUS       DAJTRQ010000450          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000450 DAJTRQ010000000
VERSION     DAJTRQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.denovo.461"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
BASE COUNT           84 a           81 c           53 g           85 t
ORIGIN      
        1 gtaggcaatg cctgttgata ttttttccat ctgatacata tcccgccccc cccccgacag
       61 acctgtgcta tcggaaagaa aaaaggccat cagcaaaact ctgatggcct gaatcacctt
      121 taccaatatt gtatgaaaaa acacgcacga ctaattgaca ataattttca tttccattaa
      181 aaaagatcgc gtagcattct taattcatga ggaactttac ccgccagcaa tctgagtagc
      241 gaaagctgtt catccccaat gtttttgctg gcgggtcctt ttttctacgg tcctcctccc
      301 cgg
//
LOCUS       DAJTRQ010000451         8256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.guided.151, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000451 DAJTRQ010000000
VERSION     DAJTRQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.guided.151"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            264..923
                     /locus_tag="OO332_004414"
     CDS             264..923
                     /locus_tag="OO332_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5944587.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OO332_004415"
     CDS             complement(1124..1501)
                     /locus_tag="OO332_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5944588.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="OO332_004416"
     CDS             complement(1568..4534)
                     /locus_tag="OO332_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5944589.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="OO332_004417"
     CDS             complement(4537..5097)
                     /locus_tag="OO332_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5944590.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="OO332_004418"
     CDS             complement(5223..5573)
                     /locus_tag="OO332_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5944591.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="OO332_004419"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="OO332_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5944592.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6949..7422
                     /locus_tag="OO332_004420"
     CDS             6949..7422
                     /locus_tag="OO332_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5944593.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            7515..>8256
                     /locus_tag="OO332_004421"
     CDS             7515..>8256
                     /locus_tag="OO332_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR5944594.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLE"
BASE COUNT         1776 a         2252 c         2409 g         1819 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt aacctctgag gaagaattgt gaaactatca
     6961 ctaatggtag ctatatcgaa gaatggagtt atcgggaatg gccctgatat tccatggagt
     7021 gccaaaggtg aacagctcct gtttaaagct attacctata accaatggct gttggttgga
     7081 cgcaagactt ttgaatcaat gggagcatta cccaaccgaa agtatgcggt cgtaacacgt
     7141 tcaagtttta catctgacaa tgagaacgta gtgatctttc catcaattaa agatgcttta
     7201 accaacctaa agaaaataac ggatcatgtc attgtttcag gtggtgggga gatatacaaa
     7261 agcctgatcg atcaagtaga tacactacat atatctacaa tagacatcga gccggaaggt
     7321 gatgtttact ttcctgaaat ccccagcaat tttaggccag tttttaccca agacttcgcc
     7381 tctaacataa attatagtta ccaaatctgg caaaagggtt aacaagtggc agcaacggat
     7441 tcgcaaacct gtcacgcctt ttgtaccaaa agccgcgcca ggtttgcgat ccgctgtgcc
     7501 aggcgttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     7561 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     7621 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     7681 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     7741 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     7801 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     7861 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     7921 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     7981 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     8041 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     8101 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     8161 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     8221 aagatcgctt ggcctcgcgc gcagatcagt tggaag
//
LOCUS       DAJTRQ010000452         7862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.guided.159, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000452 DAJTRQ010000000
VERSION     DAJTRQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.guided.159"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..359
                     /locus_tag="OO332_004422"
     CDS             <1..359
                     /locus_tag="OO332_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HCR5944595.1"
                     /translation="FSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFV
                     SANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGR
                     MLNELQQYCDEWQGGA"
     gene            complement(560..937)
                     /locus_tag="OO332_004423"
     CDS             complement(560..937)
                     /locus_tag="OO332_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5944596.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1004..3970)
                     /locus_tag="OO332_004424"
     CDS             complement(1004..3970)
                     /locus_tag="OO332_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5944597.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(3973..4533)
                     /locus_tag="OO332_004425"
     CDS             complement(3973..4533)
                     /locus_tag="OO332_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5944598.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(4659..5009)
                     /locus_tag="OO332_004426"
     CDS             complement(4659..5009)
                     /locus_tag="OO332_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5944599.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5212..6225)
                     /gene="intI1"
                     /locus_tag="OO332_004427"
     CDS             complement(5212..6225)
                     /gene="intI1"
                     /locus_tag="OO332_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5944600.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6385..6858
                     /locus_tag="OO332_004428"
     CDS             6385..6858
                     /locus_tag="OO332_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5944601.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            6951..7742
                     /gene="aadA1"
                     /locus_tag="OO332_004429"
     CDS             6951..7742
                     /gene="aadA1"
                     /locus_tag="OO332_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5944602.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1648 a         2166 c         2332 g         1716 t
ORIGIN      
        1 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
       61 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      121 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      181 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      241 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
      301 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
      361 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
      421 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      481 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      541 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
      601 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag
      661 cgccggggtt ggatttttca gcgttccagc taaggctaag gcattcctgt tcaagcgcaa
      721 gccgggctat aaagcgcatt atcgctttac ccgtgccctt atttcgatcg cactgagaaa
      781 catacagctc tttaatatgc agctgaccgc tgtatcgggg cgagggataa agaatattgc
      841 aacatgccag gccagtaatg ttattgccgc agcgcgctct gatcaccagg gtaccggaaa
      901 gccggttgaa taacttttta cagagataat ccttcatcaa cgcttcctga atgatacctt
      961 cgccataata ggggtcgtct cagaaaacgg aaaataaagc acgctaagcc ggttgcagag
     1021 gccgtagcgg cctgaacttc cccgcgccga tcttggcgct gctgcgccat aggtaatcac
     1081 cggtcaggtt gatgtgctcc cagccgagtg gcgacaggta ctgcaatagc gagtcatcga
     1141 cggcatgacc attgccgcgc aacgcatgcg ccgcacgctc caggtagacc gtgttccaca
     1201 gcacgatggc cgccgtcacc aggttgaggc cgctggcccg gtagcgctgc tgctcgaaac
     1261 tgcggtcacg gatttcacca aggcggttga agaacacggc acgggccagc gcattgcgcg
     1321 cctcgccctt gttcagcccg gcatgcacgc ggcggcgtag ctcgacgctt tgcagccagt
     1381 cgaggatgaa cagcgtgcgc tcgatgcggc ccaactcgcg cagcgcgacg gccaagccgt
     1441 tctggcgcgg gtagctgccg agtttcctga gcatcagcga ggccgtcacc gtgccctgct
     1501 tgatcgaggt ggccagccgc aggatttcgt cccaatgggc gcggacgtgc ttgatgttga
     1561 gcgtgccgcc gatcatcggc ttgagcgcgt cataggcggc atcgcccttc gggatgtaga
     1621 gcttggtgtc gcccaggtcg cggatgcgcg gcgcgaagcg gaagcccaag aggtgcatca
     1681 gggcgaagac gtgatcggtg aagcccgccg tgtcggtgta gtgctcctcg atccgcaggt
     1741 cggattcgtg gtacagcagg ccgtcgagca cgtaggttga gtcgcgcagg ccgacattga
     1801 ccaccttggt gtggaatggc gcgtattggt cggagatgtg ggtgtagaaa gtccgtcctg
     1861 ggctgctgcc atattttggg ttgatgtgcc ccgtgctctt tgccttgcta gcggttcgga
     1921 aattctgtcc gtccgatgat gatgtggtgc catcgcccca gtgcccggca aagggatgcc
     1981 gaaactgagc gttgaccagt tcagccaacg ctgtcgagta cgtttcgtcg cgggtatgcc
     2041 aggcttgcag ccaagcgagc ttcgcgtagg tcgtgccggg gcaggactcg gccatcttgg
     2101 tcaggcccag gttgatcgcg tcggccagga tcgtggtcaa caacaggttc ttgtccttgg
     2161 ccagatcgcc cgatttcaag tgcgtgaagt gccgggtgaa gcccgtccac tcatcgactt
     2221 cgagcagcag ttcggtgatc ttgacgtgcg gcaggaccat ggctgtctgg tctatcagcg
     2281 cctgcgcggt gtcgggcacc gccgcatcca gcggcgtgat cttcaagccc gactcggtga
     2341 tgatggcatc cggcaggtcg ttggctgccg ccatgcggtt gacggtggca agttgtgctt
     2401 ccagcagcgt cagccgctca tgcagatatt gttcgcagtc ggtggccacg gccagcggca
     2461 attcgctgga ctgcttgagg ctggtgaact tctcgggcgg taccaggtag tcctcgaagt
     2521 ccttgaactg gcgtgaaccc tgcacccaga tgtcgcccga gcgcagggag ttcttcaact
     2581 cggacagcgc gcacagttcg tagtagcgcc ggtcgatgcc ggcgtcggtc atcaccagtt
     2641 tctgccagcg cggcttgatg aagccggtcg gtgcatcggc tggcagcttg cgggcgttgt
     2701 cggtgttcat gccgcgcagc acctcaatgg catcaagcac gtttttggcg gcgggcgcgg
     2761 cccgcagctt gagcacggca aggaattccg gtgcatagcg gcgcagggtg gcgtagctct
     2821 cgccgatgcg atgcaggaaa tcgaagtcat cgggttgcgc gagcttctgc gcctcggtga
     2881 cgctctcggc aaaggaatcc caggacatga cggcctcgat ggcggcaaac gcatcgcggc
     2941 ctgattgctt ggcgtcgatc agcgcctgac cgatgcgccc gtacagacgt accttggcgt
     3001 tgatggcctt gcctgacgcc tggaactgct gctgatgctt attcttggca gcgttaaaca
     3061 gcttacccag gatgcggtcg tgcaggtcga tgatttcgtc ggtgacggtg gccatgccct
     3121 cggtggccag cgccacgaga gtggcgtagc gccgttgcgg ctcgaatttg gccaggtcgg
     3181 cgggtgtcat ctggccgccc tcgcgggcaa tcttgagcag gcggttctgg tgaaccagcc
     3241 gctcgatgcc ggtaggcaga tcgagtgcct gccatgcctt gaggcgttcg atgtgttcca
     3301 gcatatgccg cgaatttggc ttggccggag actggcgcaa ccaagccaac caggtcgtct
     3361 tgccgttgtc ccggcgcttg agcagatcgt cgaggcggcg gcgatgcgcg tccgccagtg
     3421 gttcggccaa ggcgtcgtag atgcgccggt tagcacgggt gatcgcctcg gcactcgccc
     3481 gctcgacggc gttgagggcg ggcagaatga ccgactgccg ccgcaggtgc ccgatcaagg
     3541 cgctggccag cacgatgcct ttgtcggttt gcatcgccag ctcggtcagc atctggacgg
     3601 cctgccggta atggctcatg gtgaagggcc ggaaaccgaa cacggtttgc agctcgctca
     3661 ggtgctcgcg ccgggtctgc tcccgctggc cgtactcgtt ccagctttcg acgccgacct
     3721 tgagctggtc ggcgaccagc ttcaacaagg gcgggaacgg tagttcatcg acgcccagga
     3781 tgacgccggg aaagcgcagg taacagagct gcaccgcgaa gcccagccga ttggctggcc
     3841 cgcgccgctg tcggatgatc gagaggtcgg tatcgttgaa tgtgtaatgt cggatcaggt
     3901 cgtccttgga gtccggcaac gccagcaggc tttcccgctc ggcggcggac aggatggaac
     3961 gacgtggcat atttactgat ccgttctcaa gtattgatac agggtttcgc gactgattcc
     4021 gaattcacga gcaagcttgg tcttttgctc gccagcctcg acacgttggc gcagttcggc
     4081 aatacgctca gacgacaggg atttcttcct gccacggtaa gccccgcgtt gcttggcgag
     4141 cgcaataccc tcgcgctgac gctcgcggat cagggcgcgc tcgaactcgg cgaacgcgcc
     4201 catcaccgag agcatcaggt tcgccatcgg agagtcttcg ccagtaaaac tgaggtgttc
     4261 cttgacgaat tcgatatgca cgccgcgttg tgtcagcgtt tgcacgatcc ggcgcaaatc
     4321 atcgagattg cgcgccaggc gatccatgct atgcaccacc acggtgtcgc cggtgcgggc
     4381 gaagcttatc agcgcttcca gttgcggacg cttgacatcc ttgccggatg ccttgtcgct
     4441 aaaagcgcga tcaaccttga cgccttccag ttgccgttcc gggttctggt cgaaggtgct
     4501 gaccctgata tacccaatgc gctgtccagt catggaattc cctgcaaaat gtcagggaag
     4561 actctatgac cttcaacgag atatgtcaat aaattcaaaa ttcaatccta tcctgacgca
     4621 atttacacat ggcatctgac atcaggttag ggtatgcctc aacctgacgg cggcgaatca
     4681 caagcgtccg gtttgacgct ggtttcggtc gcagtgctgg cccgcgcctg gaagcgctga
     4741 tagcactcca gcccgcagaa atgctccacg tactcggccc cttccggcgt gaaggcggca
     4801 tcgagcggga tttccttgca gcacacgcag caactggtgc tcggttcatt ggcgttcatg
     4861 gtggtgttcc tccattggtt gacgaagccg acgaaggccg ccgccggcat cggcctggcg
     4921 aacaggaaac cctgcaccgt gtcgcaaccc gcctgccgca accacgcaag gcagtcgggt
     4981 gtttccacac cctcggctac cacctccatt gtcgttttca gaagacggct gcactgaacg
     5041 tcagaagccg actgcactat agcagcggag gggttggatc catcaggcaa cgacgggctg
     5101 ctgccggcca tcagcggacg cagggaggac tttccgcaac cggccgttcg atgcggcacc
     5161 gatggccttc gcgcaggggt agtgaatccg ccaggattga cttgcgctgc cctacctctc
     5221 actagtgagg ggcggcagcg catcaagcgg tgagcgcact ccggcaccgc caactttcag
     5281 cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg ccgagcagat cctgcacggt
     5341 tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac gagtggcgga gggtgtgcgg
     5401 tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt ttgaaggcgc gctgaaaggt
     5461 ctggtcatac atgtgatggc gacgcacgac accgctccgt ggatcggtcg aatgcgtgtg
     5521 ctgcgcaaaa acccagaacc acggccagga atgcccggcg cgcggatact tccgctcaag
     5581 ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg tccttcagcc accatgcccg
     5641 tgcacgcgac agctgctcgc gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg
     5701 atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt
     5761 gacccgcagt tgcaaaccct cactgatccg catgcccgtt ccatacagaa gctgggcgaa
     5821 caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg gggtcagcac
     5881 caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg gcagatccgt
     5941 gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat gcgtggagac
     6001 cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa
     6061 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     6121 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     6181 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     6241 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     6301 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     6361 aaagttaacc tctgaggaag aattgtgaaa ctatcactaa tggtagctat atcgaagaat
     6421 ggagttatcg ggaatggccc tgatattcca tggagtgcca aaggtgaaca gctcctgttt
     6481 aaagctatta cctataacca atggctgttg gttggacgca agacttttga atcaatggga
     6541 gcattaccca accgaaagta tgcggtcgta acacgttcaa gttttacatc tgacaatgag
     6601 aacgtagtga tctttccatc aattaaagat gctttaacca acctaaagaa aataacggat
     6661 catgtcattg tttcaggtgg tggggagata tacaaaagcc tgatcgatca agtagataca
     6721 ctacatatat ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc
     6781 agcaatttta ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa
     6841 atctggcaaa agggttaaca agtggcagca acggattcgc aaacctgtca cgccttttgt
     6901 accaaaagcc gcgccaggtt tgcgatccgc tgtgccaggc gttaaacatc atgagggaag
     6961 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     7021 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     7081 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     7141 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     7201 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     7261 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     7321 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     7381 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     7441 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     7501 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     7561 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     7621 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     7681 atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat
     7741 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     7801 gatgcactaa gcacataatt gcggtgatgc tgccaactta ctgatttagt gtatgatggt
     7861 gt
//
LOCUS       DAJTRQ010000453         5230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.guided.199, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000453 DAJTRQ010000000
VERSION     DAJTRQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.guided.199"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            complement(<1..176)
                     /locus_tag="OO332_004430"
     CDS             complement(<1..176)
                     /locus_tag="OO332_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5944603.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIR"
     gene            438..896
                     /gene="gpt"
                     /locus_tag="OO332_004431"
     CDS             438..896
                     /gene="gpt"
                     /locus_tag="OO332_004431"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5944604.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            988..2232
                     /gene="frsA"
                     /locus_tag="OO332_004432"
     CDS             988..2232
                     /gene="frsA"
                     /locus_tag="OO332_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5944605.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2290..2691
                     /gene="crl"
                     /locus_tag="OO332_004433"
     CDS             2290..2691
                     /gene="crl"
                     /locus_tag="OO332_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5944606.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2730..3785)
                     /gene="phoE"
                     /locus_tag="OO332_004434"
     CDS             complement(2730..3785)
                     /gene="phoE"
                     /locus_tag="OO332_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5944607.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            4073..5176
                     /gene="proB"
                     /locus_tag="OO332_004435"
     CDS             4073..5176
                     /gene="proB"
                     /locus_tag="OO332_004435"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5944608.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         1284 a         1282 c         1333 g         1331 t
ORIGIN      
        1 ttacgaatca ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc
       61 caaccaacaa tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag
      121 attttggcaa aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta
      181 agtctccttg tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt
      241 tgtcaacaca agacaggctt gcgagatatg tattgagaat accactttat cccgcgtcag
      301 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
      361 atatctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
      421 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
      481 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
      541 taagccgtgg cggtctggta ccgggggcgt tactggcgcg tgaactgggt attcgtcatg
      601 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
      661 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
      721 gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
      781 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
      841 tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
      901 ttcaacgcct ggcattgccg ggcgttgttc tttttaactt caggcaggtt acaatagttt
      961 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     1021 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     1081 caaccgcctg tgcagtcggc tcttgatggt aaaaccatcc ctcactggta tcgcatgatt
     1141 aaccgtctga tgtggatctg gcgcggtatt gacccacgtg aaatcctcga cgtccaggca
     1201 cgtattgtga tgagcgatgc tgaacgtacc gacgatgatt tatacgatac cgtgattggc
     1261 taccgtggtg gcaactggat ttatgagtgg gccacccagg cgatggtgtg gcaacaaaaa
     1321 gcctgtacgg aagaagatcc gcaactcagt ggtcgacact ggctgcatgc ggctacgttg
     1381 tacaacattg ccgcctatcc tcatctgaaa ggagatgacc tggccgagca agcgcaggct
     1441 ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc cgggcacgat gcggcagatg
     1501 gagtttaccg tacccggcgg tgcgcccatc accggctttt tgcatatgcc gcaaggcgat
     1561 ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg cgatgcagac ggactattac
     1621 agcctgtatg aacgttattt tgcgccgcgc ggcattgcga tgctgactat tgatatgccg
     1681 tcggtgggct tttcttcaaa atggaagctc acccaggact ccagcctgtt gcatcagcac
     1741 gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca ctcgcgtcgc ggcctttggt
     1801 ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatacc ttgaatcgcc gcgtctgaaa
     1861 gcggttgcct gtcttggtcc ggtagttcat accctgttga gtgattttaa gtgccagcaa
     1921 caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt tggggatgca tgatgcttcc
     1981 gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa aagtgcaagg attgctgggg
     2041 cgtcgctgcc caacgccaat gttatcaggc tactggaaga acgatccgtt cagcccggaa
     2101 gaggactcac gcttaatcac ctcatcatct gctgacggta aattattaga gatcccattt
     2161 aacccggtgt atcggaattt tgacaaaggt cttcaggaaa tcaccggctg gatcgaaaaa
     2221 cgcttgtgtt aaaaatttgc taaattttgc caatttggta aaacagttgc atcacaacag
     2281 gagatagcaa tgacgttacc gagtggacac ccgaagagca gattgatcaa aaaatttacc
     2341 gcactaggcc cgtatattcg tgaaggtaag tgcgaagata atcgattctt tttcgattgt
     2401 ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc gtgaattctg gggctggtgg
     2461 atggagctgg aagcgcagga atcccgtttt acctacagct accagtttgg tctgttcgat
     2521 aaagcaggcg actggaagag tgttccggta aaagatactg aagtggttga acgactggag
     2581 cacaccctgc gtgagtttca cgagaagctg cgtgaactgc tgacgacgct gaatctgaag
     2641 ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt taacggcgtg agtgaaatgt
     2701 gccggatgca tcacatccgg caatattcat taaaactgat acgtcatccc aaccgcgaca
     2761 gtatcatcat tattaatatt cagcttatta tcgctatcca gtttgttgat tttataatca
     2821 acaaacgctg acatattttt gttgaaataa tacgtagcac cgacgtcgat ataattgacc
     2881 agatcttcat caccgatgcc ctcaatatct ttccctttcg ataagacata acccagcgat
     2941 ggacgcagac caaagtcaaa ctggtattga gcgaccgctt caaagttctg tgtcttattg
     3001 gcaaagccgc cagttattgg cgtcattttg cgtgtttcag aatagaaagt tgccagataa
     3061 atattattgg catcgtattt cagacctgtt gcccatgctt ctgcacgctt gcctgtgcca
     3121 cggctttgca ggttctgctc gttggtgcga tctgagttgg tataggcccc actaatggcg
     3181 aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga agccatcgcc gttttgcttt
     3241 ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg ttaagttcag gccatcgata
     3301 acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc tggcgcgttt ggtcataaag
     3361 ttgtcggtct gcgccgagga atcgccacca aattccggga acatatcggt ccaggcttcc
     3421 acgtcataca acgcgcccag gttacgacca taatcgaaag aacccaaatc tttatatttc
     3481 aacccggcaa aagcgagacg cgttttttgc tgtgcagtat cactctccgc tttatttccg
     3541 gcaaactccg cttcccaccg accataacca gtcagttgat cgttaatttg agtttcacct
     3601 ttaaaaccaa aacggatata actctggtcg ccatctttac tggcgttatc actcatataa
     3661 tgcatggctt taactttgcc atagacatcc agtttattac cgtctttatt atatatttct
     3721 gcagcctgta cagatgcaga tgccacaatg cccatcacca ctaatgccag agtgctcttt
     3781 ttcattttca ttcctgattt taattaacgc gcgaatattc agcgggagag ccccgttgaa
     3841 aacaggaaag tttttaacct gagattgtta aagatatatt acagattaat gatattctaa
     3901 aaatgtggta atttattaaa tctgtaataa aagcgtaaac aactgccgct acgcttgctg
     3961 atcccgcgca acaaaacgcc atgctttgct cgcagatggt tggcaaccga cgacagtcct
     4021 gctaaaacgt tcgtttgata tcatttttcc taaaattgaa tggcagagaa tcatgagtga
     4081 cagccagacg ctggtggtaa aactcggcac cagtgtgcta acaggcggat cgcgccgtct
     4141 gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg cagttacatg ccgccgggca
     4201 tcggattgtt attgtgacgt cgggcgcgat cgccgccgga cgtgagcacc tgggttaccc
     4261 ggaactgcca gcgactatcg cctcgaaaca actgctggcg gcggtagggc agagtcgact
     4321 gattcaactg tgggaacagc tgttttcgat ttatggcatt cacgtcgggc aaatgctgct
     4381 gacccgtgct gatatggaag accgtgaacg cttcctgaac gcccgcgaca ccctgcgtgc
     4441 gttgctcgat cacaatatcg ttccggtaat caatgagaac gatgctgtcg ctacggcaga
     4501 gattaaggtc ggcgataacg ataacctttc tgcgctggcg gcgattctgg cgggtgccga
     4561 taaactgttg ctgctgaccg atcaaaaagg tttgtatacc gctgatccgc gcagcaatcc
     4621 gcaggcagaa ctgattaaag atgtttacgg cattgatgac gcactgcgcg cgattgccgg
     4681 tgacagcgtt tcaggcctcg gaactggcgg catgagtact aaattgcagg ccgctgacgt
     4741 ggcttgccgt gcgggtatcg acaccattat tgccgcgggc agcaagccgg gcgttattgg
     4801 tgatgtgatg gaaggcattt ccgtcggtac gctgttccat gcccaggcga ctccgcttga
     4861 aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt gaaatcacgg tagatgaagg
     4921 ggcaactgcc gccattctgg aacgcggcag ctccctgttg ccgaaaggca ttaaaagcgt
     4981 gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc aacctcgaag gtcgcgatat
     5041 cgcccacggt gtcagtcgtt acaacagcga tgcattacgc cgtattgccg gacaccactc
     5101 gcaagaaatt gatgcaatac tgggatatga atacggcccg gttgccgttc accgtgatga
     5161 catgattacc cgttaaggag ctggccgatg ctggaacaaa tgggcattgc cgcgaagcaa
     5221 gcctcgtata
//
LOCUS       DAJTRQ010000454         4549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.guided.209, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000454 DAJTRQ010000000
VERSION     DAJTRQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.guided.209"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            16..1149
                     /locus_tag="OO332_004436"
     CDS             16..1149
                     /locus_tag="OO332_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5944609.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            1238..1771
                     /gene="blc"
                     /locus_tag="OO332_004437"
     CDS             1238..1771
                     /gene="blc"
                     /locus_tag="OO332_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5944610.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1768..1997)
                     /locus_tag="OO332_004438"
                     /pseudo
     CDS             complement(1768..1997)
                     /locus_tag="OO332_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2173..2319)
                     /gene="ecnB"
                     /locus_tag="OO332_004439"
     CDS             complement(2173..2319)
                     /gene="ecnB"
                     /locus_tag="OO332_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5944611.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2430..2555)
                     /gene="ecnA"
                     /locus_tag="OO332_004440"
     CDS             complement(2430..2555)
                     /gene="ecnA"
                     /locus_tag="OO332_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5944612.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2607..3173)
                     /gene="efp"
                     /locus_tag="OO332_004441"
     CDS             complement(2607..3173)
                     /gene="efp"
                     /locus_tag="OO332_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5944613.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3215..4243
                     /gene="epmB"
                     /locus_tag="OO332_004442"
     CDS             3215..4243
                     /gene="epmB"
                     /locus_tag="OO332_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5944614.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1170 a         1145 c         1129 g         1105 t
ORIGIN      
        1 tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttgc
       61 tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt
      121 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
      181 tactttactt ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
      241 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct
      301 cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact taccgctaaa
      361 cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg cctgccattg
      421 caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
      481 cctgcatggg ccccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
      541 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac tcgtgtcttc
      601 cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga aaagaattac
      661 gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt agatgctgaa
      721 gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag caatttaccc
      781 ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca atctcgctac
      841 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtcaat
      901 cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg cccggtaaaa
      961 gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac gggggcgacc
     1021 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat cgtgatgctg
     1081 gctaacaaaa actatcccaa tccagagaga gtcgccgccg cctgtcagat tctcaacgct
     1141 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
     1201 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
     1261 cgcagcgaca gctgcatttc tggtcgttgc ctgtagttct cctacgccgc cgcgtggcgt
     1321 gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg
     1381 ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga
     1441 tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca
     1501 gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt
     1561 ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc
     1621 gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc
     1681 tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa
     1741 gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc
     1801 caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac cgagcagcac
     1861 aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat caaacggcta
     1921 aagccgtggg tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact
     1981 aagataatcc aggacatatc aggctccgga aatttggggc cgtccccggt gaaaagaggc
     2041 gtttgcaggt cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga
     2101 atttcaacta ttacctttat gacgatcgca gaaaaaacaa acggcacgac actgctgtcg
     2161 taccgttatt gcttattgct gcgctttcgt tgctgcgcca gaaatcgcgt taccgccatc
     2221 agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg ttgaaagcac
     2281 cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct tttaggtaat
     2341 gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa atgatggaaa
     2401 tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgagagat ggagttgccg
     2461 agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac agcccgtgag cagcgtgctg
     2521 gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa gtgatggtgc
     2581 agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc accagagcgg
     2641 gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac cacagcgcca
     2701 gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag gcccggatcg
     2761 gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg ctgaccattc
     2821 cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc gccaattgct
     2881 tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca gaactcaccg
     2941 tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc ggaatcagta
     3001 gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt aacgcgagca
     3061 aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta aggttcgccg
     3121 tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc cataaggccc
     3181 tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa ccctaaatac
     3241 cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc ctgatgaact
     3301 tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca gcgccaaaaa
     3361 gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag gcaatccgga
     3421 cgatcctctt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg cgtccggatt
     3481 ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc ataaatacca
     3541 caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct attgcttccg
     3601 tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga ctgcacttga
     3661 gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg atccgctgat
     3721 ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc cgcatatcaa
     3781 acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca ccgaggcgct
     3841 ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca tcaatcatgc
     3901 caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg tcggtgtcac
     3961 cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa cgttggcaaa
     4021 cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg tgctagataa
     4081 agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga ttatgcgtga
     4141 gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa ttggcggcga
     4201 acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta aacagggaag
     4261 ttaaacatta aaattgcctg atgcgctacg cttatcggcc tacatcgtgc attgaaattg
     4321 aatggatttg caatgaactg taggccggat aaggcgttta cgccgcatcc ggcaagagca
     4381 acaaacgcaa gtacagcaac ctgtcattaa actgagaaat ttaatctttg cacgttgcat
     4441 aacctttaat tattcatatg cattgctgtt gttacttttt atatagctaa caccaataat
     4501 taacctttct tttcggtaat gactccaact tactgatagt gttttatgt
//
LOCUS       DAJTRQ010000455         2510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.guided.243, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000455 DAJTRQ010000000
VERSION     DAJTRQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.guided.243"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            <1..206
                     /locus_tag="OO332_004443"
     CDS             <1..206
                     /locus_tag="OO332_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5944615.1"
                     /translation="DVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTR
                     HLGYREVTITEKGYAAFKTHFHI"
     gene            complement(184..807)
                     /gene="tetR(B)"
                     /locus_tag="OO332_004444"
     CDS             complement(184..807)
                     /gene="tetR(B)"
                     /locus_tag="OO332_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5944616.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            889..2094
                     /gene="tet(B)"
                     /locus_tag="OO332_004445"
     CDS             889..2094
                     /gene="tet(B)"
                     /locus_tag="OO332_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5944617.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2207..>2510)
                     /locus_tag="OO332_004446"
     CDS             complement(2207..>2510)
                     /locus_tag="OO332_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5944618.1"
                     /translation="DYIIDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKL
                     IQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          641 a          483 c          531 g          855 t
ORIGIN      
        1 ttgacgttcc ttccaaacat tcacgtaaaa tctgttgtgc gtgtttagat tggagtgaac
       61 gccgtttcca tttaggtggg tacgttggag ccgcattatt ttcgctttat gaatctaaag
      121 ggtggttaac tcgacatctt ggttaccgtg aagttaccat cacggaaaaa ggttatgctg
      181 cttttaagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      241 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      301 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      361 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      421 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      481 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      541 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      601 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      661 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
      721 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
      781 cactttactt ttatctaatc tagacatcat taattcctaa tttttgttga cactctatca
      841 ttgatagagt tattttacca ctccctatca gtgatagaga aaagtgaaat gaatagttcg
      901 acaaagatcg cattggtaat tacgttactc gatgccatgg ggattggcct tatcatgcca
      961 gtcttgccaa cgttattacg tgaatttatt gcttcggaag atatcgctaa ccactttggc
     1021 gtattgcttg cactttatgc gttaatgcag gttatctttg ctccttggct tggaaaaatg
     1081 tctgaccgat ttggtcggcg cccagtgctg ttgttgtcat taataggcgc atcgctggat
     1141 tacttattgc tggctttttc aagtgcgctt tggatgctgt atttaggccg tttgctttca
     1201 gggatcacag gagctactgg ggctgtcgcg gcatcggtca ttgccgatac cacctcagct
     1261 tctcaacgcg tgaagtggtt cggttggtta ggggcaagtt ttgggcttgg tttaatagcg
     1321 gggcctatta ttggtggttt tgcaggagag atttcaccgc atagtccctt ttttatcgct
     1381 gcgttgctaa atattgtcgc tttccttgtg gttatgtttt ggttccgtga aaccaaaaat
     1441 acacgtgata atacagatac cgaagtaggg gttgagacgc aatcgaattc ggtatacatc
     1501 actttattta aaacgatgcc cattttgttg attatttatt tttcagcgca attgataggc
     1561 caaattcccg caacggtgtg ggtgctattt accgaaaatc gttttggatg gaatagcatg
     1621 atggttggct tttcattagc gggtcttggt cttttacact cagtattcca agcctttgtg
     1681 gcaggaagaa tagccactaa atggggcgaa aaaacggcag tactgctcgg atttattgca
     1741 gatagtagtg catttgcctt tttagcgttt atatctgaag gttggttagt tttccctgtt
     1801 ttaattttat tggctggtgg tgggatcgct ttacctgcat tacagggagt gatgtctatc
     1861 caaacaaaga gtcatcagca aggtgcttta cagggattat tggtgagcct taccaatgca
     1921 accggtgtta ttggcccatt actgtttgct gttatttata atcattcact accaatttgg
     1981 gatggctgga tttggattat tggtttagcg ttttactgta ttattatcct gctatcgatg
     2041 accttcatgt taacccctca agctcagggg agtaaacagg agacaagtgc ttagttattt
     2101 cgtcaccaaa tgatgttatt ccgcgaaata taatgaccct cttgataacc caagagggca
     2161 ttttttacga taaagaagat ttagcttcaa ataaaaccta tctattttat ttatctttca
     2221 agctcaataa aaagccgcgg taaatagcaa taaattggcc ttttttatcg gcaagctctt
     2281 ttaggttttt cgcatgtatt gcgatatgca taaaccagcc attgagtaag tttttaagca
     2341 catcatcatc ataagcttta agttggttct cttggatcaa tttgctgaca atggcgttta
     2401 ccttaccagt aatgtattca aggctaattt tttcaagttc attccaacca atgataggca
     2461 tcacttcttg gatagggata aggtttttat tattatcaat aatataatca
//
LOCUS       DAJTRQ010000456         1641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600558
            SAMEA8900372-rid15818653.guided.262, whole genome shotgun sequence.
ACCESSION   DAJTRQ010000456 DAJTRQ010000000
VERSION     DAJTRQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900372
            Sequence Read Archive: ERR6004776
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477690.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477690.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 22:01:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,448
            CDSs (total)                      :: 4,377
            Genes (coding)                    :: 3,987
            CDSs (with protein)               :: 3,987
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 202 of 390
            Pseudo Genes (incomplete)         :: 98 of 390
            Pseudo Genes (internal stop)      :: 135 of 390
            Pseudo Genes (multiple problems)  :: 41 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900372-rid15818653.guided.262"
                     /serovar="S. flexneri 3a"
                     /isolate="600558"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-05-20"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OO332_004447"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OO332_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5944619.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1477..>1641
                     /gene="glmM"
                     /locus_tag="OO332_004448"
     CDS             1477..>1641
                     /gene="glmM"
                     /locus_tag="OO332_004448"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5944620.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSG"
BASE COUNT          369 a          454 c          441 g          377 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc
     1441 ctttgagggc gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca
     1501 gacggtattc gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc
     1561 ggcatggcgg ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc
     1621 aaggatacgc gcctgtcggg c
//
